LOCUS       DAVMEF010000001        40958 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMEF010000001 DAVMEF010000000
VERSION     DAVMEF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.001"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            378..995
                     /locus_tag="VYQ30_000001"
     CDS             378..995
                     /locus_tag="VYQ30_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1965183.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(1315..2202)
                     /locus_tag="VYQ30_000002"
     CDS             complement(1315..2202)
                     /locus_tag="VYQ30_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1965184.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(2351..4234)
                     /locus_tag="VYQ30_000003"
     CDS             complement(2351..4234)
                     /locus_tag="VYQ30_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1965185.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLLNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTSMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKRAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            4611..6065
                     /locus_tag="VYQ30_000004"
     CDS             4611..6065
                     /locus_tag="VYQ30_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1965186.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            6046..7011
                     /locus_tag="VYQ30_000005"
     CDS             6046..7011
                     /locus_tag="VYQ30_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1965187.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(7046..8320)
                     /locus_tag="VYQ30_000006"
     CDS             complement(7046..8320)
                     /locus_tag="VYQ30_000006"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1965188.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVVLYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(8320..9492)
                     /locus_tag="VYQ30_000007"
     CDS             complement(8320..9492)
                     /locus_tag="VYQ30_000007"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1965189.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(9623..9871)
                     /locus_tag="VYQ30_000008"
                     /pseudo
     CDS             complement(9623..9871)
                     /locus_tag="VYQ30_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9984..10565)
                     /locus_tag="VYQ30_000009"
                     /pseudo
     CDS             complement(9984..10565)
                     /locus_tag="VYQ30_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10627..11076)
                     /locus_tag="VYQ30_000010"
     CDS             complement(10627..11076)
                     /locus_tag="VYQ30_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1965190.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(11078..11836)
                     /locus_tag="VYQ30_000011"
     CDS             complement(11078..11836)
                     /locus_tag="VYQ30_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1965191.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(11951..13930)
                     /gene="pknB"
                     /locus_tag="VYQ30_000012"
     CDS             complement(11951..13930)
                     /gene="pknB"
                     /locus_tag="VYQ30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1965192.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(13927..14667)
                     /locus_tag="VYQ30_000013"
     CDS             complement(13927..14667)
                     /locus_tag="VYQ30_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1965193.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLINELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(14682..15995)
                     /gene="rsmB"
                     /locus_tag="VYQ30_000014"
     CDS             complement(14682..15995)
                     /gene="rsmB"
                     /locus_tag="VYQ30_000014"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1965194.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(15988..16923)
                     /locus_tag="VYQ30_000015"
     CDS             complement(15988..16923)
                     /locus_tag="VYQ30_000015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1965195.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEVLPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(16936..19332)
                     /locus_tag="VYQ30_000016"
     CDS             complement(16936..19332)
                     /locus_tag="VYQ30_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1965196.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(19398..19712)
                     /locus_tag="VYQ30_000017"
     CDS             complement(19398..19712)
                     /locus_tag="VYQ30_000017"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1965197.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(19737..20363)
                     /gene="gmk"
                     /locus_tag="VYQ30_000018"
     CDS             complement(19737..20363)
                     /gene="gmk"
                     /locus_tag="VYQ30_000018"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1965198.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(20493..22106)
                     /locus_tag="VYQ30_000019"
     CDS             complement(20493..22106)
                     /locus_tag="VYQ30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1965199.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(22297..22551)
                     /locus_tag="VYQ30_000020"
     CDS             complement(22297..22551)
                     /locus_tag="VYQ30_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV1965200.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(22555..22809)
                     /locus_tag="VYQ30_000021"
     CDS             complement(22555..22809)
                     /locus_tag="VYQ30_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1965201.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(23047..24144)
                     /locus_tag="VYQ30_000022"
     CDS             complement(23047..24144)
                     /locus_tag="VYQ30_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1965202.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILTWLGIDSLAFGTEEVLD
                     YQKIADLYTEQGAEMENFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGYNIKLHPIQRQGAGYHSVDKDVNFASATALRQHQRDKDFLERFMPSVALFEQA
                     SKVSWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQVRESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(24154..24900)
                     /locus_tag="VYQ30_000023"
     CDS             complement(24154..24900)
                     /locus_tag="VYQ30_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1965203.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDETPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(25003..25356)
                     /gene="rsfS"
                     /locus_tag="VYQ30_000024"
     CDS             complement(25003..25356)
                     /gene="rsfS"
                     /locus_tag="VYQ30_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1965204.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(25369..25869)
                     /locus_tag="VYQ30_000025"
     CDS             complement(25369..25869)
                     /locus_tag="VYQ30_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1965205.1"
                     /translation="MKTSFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(25870..26463)
                     /gene="yqeK"
                     /locus_tag="VYQ30_000026"
     CDS             complement(25870..26463)
                     /gene="yqeK"
                     /locus_tag="VYQ30_000026"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1965206.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(26463..27092)
                     /locus_tag="VYQ30_000027"
     CDS             complement(26463..27092)
                     /locus_tag="VYQ30_000027"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1965207.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(27144..27455)
                     /gene="yhbY"
                     /locus_tag="VYQ30_000028"
     CDS             complement(27144..27455)
                     /gene="yhbY"
                     /locus_tag="VYQ30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1965208.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(27669..28775)
                     /gene="yqeH"
                     /locus_tag="VYQ30_000029"
     CDS             complement(27669..28775)
                     /gene="yqeH"
                     /locus_tag="VYQ30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1965209.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTDDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAREEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(28778..29368)
                     /locus_tag="VYQ30_000030"
     CDS             complement(28778..29368)
                     /locus_tag="VYQ30_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1965210.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(29320..30228)
                     /locus_tag="VYQ30_000031"
     CDS             complement(29320..30228)
                     /locus_tag="VYQ30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1965211.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(30285..30845)
                     /locus_tag="VYQ30_000032"
     CDS             complement(30285..30845)
                     /locus_tag="VYQ30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1965212.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            30984..32202
                     /gene="sstT"
                     /locus_tag="VYQ30_000033"
                     /pseudo
     CDS             30984..32202
                     /gene="sstT"
                     /locus_tag="VYQ30_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            32270..33223
                     /locus_tag="VYQ30_000034"
     CDS             32270..33223
                     /locus_tag="VYQ30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1965213.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(33459..33539)
                     /locus_tag="VYQ30_000035"
                     /pseudo
     CDS             complement(33459..33539)
                     /locus_tag="VYQ30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(33594..33818)
                     /locus_tag="VYQ30_000036"
     CDS             complement(33594..33818)
                     /locus_tag="VYQ30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEV1965214.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(33823..34002)
                     /gene="asp4"
                     /locus_tag="VYQ30_000037"
     CDS             complement(33823..34002)
                     /gene="asp4"
                     /locus_tag="VYQ30_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEV1965215.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(34004..35341)
                     /gene="gtfB"
                     /locus_tag="VYQ30_000038"
     CDS             complement(34004..35341)
                     /gene="gtfB"
                     /locus_tag="VYQ30_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEV1965216.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(35334..36845)
                     /gene="gtfA"
                     /locus_tag="VYQ30_000039"
     CDS             complement(35334..36845)
                     /gene="gtfA"
                     /locus_tag="VYQ30_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEV1965217.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(36857..39229)
                     /gene="secA2"
                     /locus_tag="VYQ30_000040"
     CDS             complement(36857..39229)
                     /gene="secA2"
                     /locus_tag="VYQ30_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEV1965218.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLL
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWIEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(39222..39662)
                     /gene="asp3"
                     /locus_tag="VYQ30_000041"
     CDS             complement(39222..39662)
                     /gene="asp3"
                     /locus_tag="VYQ30_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEV1965219.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGSTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(39659..>40958)
                     /gene="asp2"
                     /locus_tag="VYQ30_000042"
     CDS             complement(39659..>40958)
                     /gene="asp2"
                     /locus_tag="VYQ30_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV1965220.1"
                     /translation="QNLQINDREVVDFLKKRCAQAIDFLSPQQLINDLSKSLFGGGYG
                     DKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFAQLAYWAYNIMVQKTLPIELWL
                     EYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLEQALFMDSRDGDYFLSISVEAR
                     GRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEINYFFHPGDFKPPLTVYFAGYR
                     PAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDELEGKVKDTITHYLDYLGFDHK
                     DLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGTIASRGRLDAPGVSNLAFDCLI
                     HHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYMKDEEMDPQAYEQLVSYLCNTG
                     AKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGREKR"
BASE COUNT        11512 a         9441 c         7862 g        12143 t
ORIGIN      
        1 tgattaatat taaaattaac taacaattca aaggattcat actagccata aattacgtcc
       61 atcagagaga gactcttact acttttagat tttagtcttt ctagcttcag aatacatcta
      121 aactttagga aaaatgacta ttcgaaagcg cgaatgcctc aaaattatct cagataagct
      181 attcgaaact tagaatgctt ttaaatttat ggaattgcga ttattcgaaa cctagaatgc
      241 atataacctt tagttgacag acctattcta agtctcgaag ggctatttac tttctattcc
      301 ttatcaaaaa agactcattc cccctttctc ctccaaaata tggtatagta gaaatatact
      361 atctatgagg agtttacatg tcacaggata aacaaatgaa agctgtttct ccccttctgc
      421 agcgagttat caatatctca tcgattgtcg gtggggttgg gagtttgatt ttctgtattt
      481 gggcttatca ggctgggatt ttacaatcca aggaaaccct ctctgccttt atccagcagg
      541 caggcatctg gggtccacct ctctttatct ttttacagat tttacagact gtcgtcccta
      601 tcattccagg ggccttgacc tcggtggctg gggtctttat ctacgggcac atcatcggga
      661 ctatctacaa ctatatcggc atcgtgattg gctgtgccat tatcttttat ctagtgcgcc
      721 tatacggagc tgcctttgtc cagtctgtcg tcagcaagcg cacctacgac aagtacatcg
      781 gctggctaga taagggcaat cgttttgacc gcttctttat ttttatgatg atttggccca
      841 ttagcccagc tgactttctc tgtatgctgg ctgccctgac caagatgagt ttcaagcgct
      901 acatgaccat catcattctg accaaaccct ttaccctcgt ggtttatacc tacggtctga
      961 cctatattat tgactttttc tggcaaatgc tttgacacgt aaaaaatccg tttggtttcc
     1021 caagtggatt tttaaagcgt agattaacta tagcttgata ctaaatatac tttggtatgg
     1081 aaatcatgca tatttttcga tagtgaggcg aggacttacc tagcctttcc gccgtgatag
     1141 aaacacctga aatctaatgg tttcaggtat tcggaaactt tgagcctagt gtctcaaagt
     1201 ttaggtatgg aatttcgaag aaagtcgcta ccgtccgtaa tcacttaagg aaaggctcaa
     1261 aaatattgtt ttcaaccaca aaatccgttt ggtttcccaa gcggattttg tgctttattt
     1321 tgaaacttct tttgcaagaa caaagttccc aagtgtggca gaaccatttc ctgcgactgc
     1381 tggcgtcacg atatagtcac gcacatctgg tactggtagg taaccattaa gaagagatgt
     1441 aaatttctca cggacacggt ccagcatatg ttgttgagcc atgacccctc caccaaagac
     1501 aatcacgtct gggcggaaag tcactgtcgc attaaccgca gcttgagcga tatagtaggc
     1561 ttgaacatcc caaacagggt tgttgagttc aatagtttcc ccacgtacac ctgtacgagc
     1621 ttccaaactt ggaccagctg cataaccttc tagacatccc ttatggaaag gacaaacacc
     1681 cttaaactct ttttcaatat ccattgggtg tctagcaaca taataatgac ccatttcagg
     1741 gtgacccaca ccaccgataa actcaccacg ttggatgacg cctgcaccga tacctgtacc
     1801 gattgtgtag taaaccaagt tttcgatacg accaccagca ttgttacggg caaccatttc
     1861 accgtaagca gagctgttta cgtctgttgt gaagtacatt ggcacgttta gggcgcgacg
     1921 aagggcacca agcaagtcta catttgccca gtttggtttt ggagtcgtcg tgataaagcc
     1981 ataagttttt gagtttttgt caatatcaat cggcccaaat gaaccaactg caagaccagc
     2041 aaggttatcg aattttgaga agaactcaat ggttttatcg attgtttcga ttggagttgt
     2101 tgttggaaat tgtgtttttt ctacaacgtt aaagttttca tcaccgacag cacagacaaa
     2161 ctttgtaccg cccgcttcca agcttccata taattttgtc atgataaacc tcttgttttt
     2221 attttcttta ttatagcata cttcgaaagt ctaaatgtct ctatttttta gattttcctc
     2281 tgtaaatctt actatctaat aaaaacgaac aaacatgtca tttgttcgtt ttcacattag
     2341 agaggattga ttagattttc acttcgatca cagcatcccc cttagcaact gaacctgttg
     2401 cgactggagc tactgaagcg tagtcacctg tatttgtaac gataaccatt gttgtatcat
     2461 caagtccagc tgcagcgatt ttgtttgagt caaatgttcc aagaacatcg ccagctttca
     2521 ccttattacc ttgagcaact tttgtttcaa aaccgtcacc gttcatagat acagtatcaa
     2581 taccaacatg aatcaaaact tcagcaccat ttcttgtttt caaaccaaaa gcgtgccctg
     2641 ttggaaaggc aattgaaact tcagcatcag ctggtgcata gaccacgcct tggcttggtt
     2701 tcacagcgat accttgtccc atagctccac ttgagaagac tgggtcattg acatcagcaa
     2761 gagcgacaac atcaccgacg ataggagtta caagtgtttc attttgaaga gctgctggcg
     2821 caacttcttc tttttcttca gccacttcag ctcgttttgc agctgcagtt gcgtctactt
     2881 catcttcgta accaaacatg taagtaagag caaaaccaag ggcaaatgat acagctacca
     2941 taagaaggta ttgtggaagt tgtccgttac caacataaag cattgtacca gggatgatgg
     3001 tgataccatt accagtacca gcaagtccaa ggatagaagc caatccacca ccgattgcac
     3061 cagcaatcaa tgaaaggaag aatggtttac ggaagcgcaa gttcaccccg aagatagcag
     3121 gctctgtaat acctaggaag gcagaaagag cagccgggaa agcaagtgtt ttcagttttg
     3181 gattttttgt tttaacacca accgcaacag tagcagcacc ttgagctgtc atagcagctg
     3241 tgatgatagc gttgaatggg ttagcatggt cagcagcaag taattgcact tcaagcaagt
     3301 tgaagatgtg gtgcacacct gacacgacga tcaattggtg aaccccacca atcaagaaac
     3361 caccaagacc aaatggcatg ctaagaatcg cttttgtagc aataaggatg tagttttcaa
     3421 caacgtggaa aactggtcca atgacaaaga gtccaaggat agacatgacc aaaagtgtca
     3481 cgaatggtgt taccaagagg tcaatgacat ctggaacaac cttgcggaca gctttttcaa
     3541 atttagctcc gacaaccccg atgatgaagg ctggaagaac ggaaccttgc aaaccaacaa
     3601 cagggatgaa accaaagaag ttcatcgatg ttacttcacc accttgagca actgcccaag
     3661 cgtttggaag tgagccagag acaagcatca taccaagaac gataccaacg gcaggatttc
     3721 caccaaatac acggaaggtt gaccacacaa ccaaacctgg caagatgatg aaggctgtat
     3781 ctgtcaagat ttgtgtgtaa gttgcaaagt cacctggaag tggcatttca agagcgttga
     3841 gaagaccacg cacacccatg aagagacctg tcgctacgat aactgggatg attggaacga
     3901 aaacatcacc gaaagtacgg atagcacgtt ggaaccagtt cccttgttta gcaacttctg
     3961 ctttcatgtc atccttagat gatgttggta agccaagtac aacaacttca tcgtacattt
     4021 tgttaactgt acctgtacca aagataattt ggtattgccc tgagttaaag aaagcacctt
     4081 gaactttttc caagttctca atcacttctt tattgatttt ctcttcatct ttgaccatga
     4141 cacgtagacg agtcgcacag tgggcaacac tattgacatt ttcacgtccg cccaaggcat
     4201 cgatgacttt ttttgcaatt tcctgattgt tcatttgcaa aaatctcctt atataacatt
     4261 ttgttcttgt ttgaaagcga ttttattcgc ccttacgact attattttat catgttttaa
     4321 aaatatgtca agcgttttgc agaaaaaata ttctatccac ttagtgggca agctttagat
     4381 tgattgtttt tgactgtttc atcaggaatt tcttgaaaaa taagtttgaa accttggtta
     4441 aaaagtcttt tatttcatta tctttcacat tttcgtaaaa atttgattga tttcctgtat
     4501 ttattttatg gtaatcgttt gacagttttt tctctttttt aatataaaag gcttgcattt
     4561 tttatcgaaa acagttacta ttaaaagtga taagattttt ggaggaatga atggaatgga
     4621 caactgagcg tcgttacaga ctttatcaag attggacgca agaagaaatt caacatataa
     4681 aggaaaatat ggcacaatct ccatggcata ctcattacca tgttgagcca aaaacaggac
     4741 ttctcaacga cccaaatggc ttttcttact ttgatggcaa gtggatcctc ttttaccaga
     4801 attttccttt tggtgcagcc cacggtttga aatcttgggc acagctagaa agtgatgatt
     4861 tggttcactt taaagaaact ggaatcaaag ttttaccaga tactccatta gatagccacg
     4921 gtgcctactc tggttctgcc atgcaatttg gcgataactt attcctattt tatacaggaa
     4981 atgttcgcga tgaaaactgg atccgtcacc cataccagat cggtgctttg atggacaagg
     5041 agggtaagat tacaaagatt gacaagatct tgattgacca gccagcagac tctactgacc
     5101 acttccgcga tccacaaatt tttaacttta aaggtcaata ctatgctgtc gtcggtggac
     5161 aggacttgga gaaaaaaggt ttcgttcgtc tctacaaggc tgtcaataac gactacacaa
     5221 actggcaagc agttggcgac cttgactttg ctaacgaccg tactgcctac atgatggaat
     5281 gtcctaatct ggtctttgta gaggaacaac ctgtccttct ctactgtcca caaggattgg
     5341 ataagaaagt tctagactac gataatatct ttccaaatat gtataagatt ggggcttcct
     5401 ttgaccctaa aaatgccaaa atggtagatg tgtctcaact tcaaaacatg gattacggtt
     5461 tcgaagccta tgcaactcaa gccttcaacg ctcctgatgg gcgtgctcta gcagttagct
     5521 ggcttggttt gccagatgtt tcttacccat ctgaccgttt tgaccaccaa ggaaccttct
     5581 ctttggtcaa ggaactcact atcaaagacg acaagctcta ccagtatcca gtcgctgcta
     5641 ttaaggacct tcgtgcttct gaagaagcct tctcaaaccg ttcccaaacc aagaacactt
     5701 acgaacttga actcaacttg gaagctaata gccagagcga gattgtctta cttgctgata
     5761 aagaaggtaa gggactttca atcaactttg accttgtaaa cggtcaagtg acagtggatc
     5821 gtagccaggc tggagaacag tatgcccaag aatttgggac aactcgttct tgccctatcg
     5881 agaatcaggc tactactgct acaatcttca tcgataactc tgtctttgaa attttcatca
     5941 ataaaggaga aaaagtattt tctggtcgtg tcttcccaca tgcggaccaa aatggtatcc
     6001 tgatcaaatc tggaaaccca actggaactt actatgaatt agattatggt cgcaaaacta
     6061 actgatgtcg ccaaacttgc aggcgtcagt cctactaccg tttctcgggt tatcaataaa
     6121 aaagggtatc tatctgagaa aaccatccaa aaagtcaatg aagccatgcg agaattgggc
     6181 tataaaccca acaacctggc tcgtagtctg caaggaaaat cagctaagtt aatcggcttg
     6241 attttcccta atatttccaa tgttttctat gcagaattga ttgataaatt ggaacaccaa
     6301 ctcttcaaaa atggttacaa gaccatcatc tgcaacagtg aacatgattc tgagaaggaa
     6361 cgcgaataca tcgaaatgtt ggaagccaat caggtggacg gcatcatttc tggtagtcac
     6421 aacctaggaa tcgaagacta caatcgtgtg acagcgccga ttatttcctt tgaccgaaac
     6481 ctatcgccag acatccctgt cgtctcctct gacaactatg ctggtggggt tcttgctgcc
     6541 caaaccttgg tcaagacagg tgcccagtct atcatcatga ttacagggaa tgacaattcc
     6601 aattcgccaa ccggactacg ccacgctggt tttgcatccg tactcccaaa agctcctatt
     6661 atcaatgttt ccagtgactt ttctccagtc agaaaagaaa tggaaatcaa gaatatcttg
     6721 acccgggaaa aaccagatgc catttttgct tcggatgatt tgacagctat tctggtcatt
     6781 aaaatcgctc aagaattggg catttctgtc ccaaaagagc tcaaggtcat cggctatgat
     6841 gggacctact ttatcgaaaa ttactaccct caattggcta ctatcaagca acctttggaa
     6901 gagattgctt gtctcactat tgatcttctc ttgcaaaaga ttgaaggcaa ggaagtcgcc
     6961 acaactggtt acttcttacc agttacgcta ttaccaggaa aaagtattta aacacaagaa
     7021 aactcagacc gattcgtctg agtttttatg atcttaaatt ttcgagatag cgctgggctg
     7081 tctctaggtt aaaggtttta tctgagatga ggcgctctac taggggagca acttcagatt
     7141 cactagcccc agctaggaga gctagggatt tggcctgtag tttcatgtgg ccttgctgga
     7201 tgcccgtact taccaaggct ttgagggctg caaaattttg agcaagaccg atggacacga
     7261 taatctgggc taattctctg gcagaaggat ttcctagtag atcatgactg agagctacac
     7321 gtgggttgag gccgatagag ccacccttag tcgctacagg catgggcagg gtcatctcac
     7381 cgaccaattc ttctctttca aggtccagcg tccagcagct aagaccttga tagcgtccat
     7441 ctcgactggc aaaggcatgg gccccagctt cgatggcacg ccagtcatta ccagtggcaa
     7501 tcaaaatcgc atcaatacca ttaaaaattc ctttattatg agtagcagct cggtaaggat
     7561 cagcctgcgc aaactgacta gccaacgcaa ttttctccgc aatctctcgt ccttgatcct
     7621 tttggcggct caagtagcga aaggcgatgc gacagcttgc agtcaccaga gaatcagtcg
     7681 cgtagttgga caggattccc atgagactct gtccctgact gagttcttct aagactggtt
     7741 tcaaggcttc cagcatggtg ttgagcatat tggcacccat ggcttcctgg gtatcgacat
     7801 gaatataaac aacgagaaag tctggttcgc cttttatctg ctcgacatgc agatcacgcg
     7861 ccccaccccc acgtttaacg atagaaggat aggcttgatt ggcaagctcc aagagctccg
     7921 ctttcttgct ggcaatcttc tcttgcgcta gtttaggatt agcaacttga taaaggacta
     7981 cctgcccaat catctggcgc tgatggactt gtgcagtaaa accacctgca cgcttgatga
     8041 ttttgctggc atagctggcc gccgcaacca cagagggttc ttctgtcaca tagggaacgg
     8101 tgtattcctg accgttgaca agtacctccg gaaccagcga ataaggcaga gaaaaagttc
     8161 ccaccacatt ctcactcagc tggtctgcca cagtcacact catctgttca tccttctcca
     8221 gactagcttg tctctcagga ctaaggagcg cctgagcttt tagcagctcg aggcgctctt
     8281 ggtatgattt tttagaaaat ccattccaac ttatcttcat tatttttcaa ccttgctata
     8341 acggcgttgg tggtcaagaa tttcaaccaa ggcaaaatct tgattttcat agccagcaaa
     8401 ctgggcagag tttgtttcat ccaagtttac ttcctcaaaa aagacctttt catagtctgc
     8461 aacggatagg gcagttcgtt ggttgagctt gttcaagcgg tctttatcca aataagcttc
     8521 atatccttca accaattcac cactgaagaa ctcagccaca gctccacttc cgtaactata
     8581 aagggcgatt ttatccccag ctttcaagct atctgtattt tccaagagag acaaaagtcc
     8641 aaggaaaagt gaacctgtgt agatattccc caccttttga ctgtagagaa tagactggtc
     8701 aaaatgcttt tgtaagaggt cttttttctc ttgaggcagg ctcttatcca tgattttttt
     8761 caagcctttt agcgctaatt taggataagg caagtggaaa caaacagccg caaaatcatc
     8821 caaagtaagc tggtagcgtt tttgatattc aagccaagtc gttttcaaac tatccaagta
     8881 ttgttgggta gaatagacac catttacata aggagttgtc gagtaatttg gtcgccagaa
     8941 atccatgatg tcacgggtct gagctacatt gtcattatta aaggccatca tgcgtggatt
     9001 ttgtgtaatc aacatagcta cacttccagc accttgagtt ggttctcctg gagtttcaat
     9061 accgtatttg gcaatatcac tggcaatgac caagaccttg gactccggag aattttccac
     9121 atgcaatttg gcataatgaa gggcagcagt cgctccataa caggcttctt taatctcgaa
     9181 actacgagca aagggctgga tgcccagcaa gccatgcaca aagacggccg cagccttact
     9241 ctggtcaatt cctgactcgg tagccacaat gaccatgtca acttcttgtc tttcttgctc
     9301 agttaaaata gagtcactag cactggccgc caaggtcaca atgtcctcag ttaggggcgc
     9361 aatactcaat tccttgagta agagtccttt acttaatttt tcagggtcaa ttcccctcgc
     9421 ttctgctaag tcttgtaatt tcaagacata ttgactggtc gcaaaaccaa tcttatcaat
     9481 accgattgtc atatttacct ctgttttatc attcatgtaa aaaatcgttc tatactattt
     9541 tatcacaaat ggcagtaaaa gagagaaaaa agacttgatt caccaaatca agcctcttat
     9601 tggtcatcat tttaaagaat gattagttgc tagaaagttc accgatataa gtagctttat
     9661 aagcttcatt cacagttatc agctcctgga ggatcaaatt tcctgagtaa gtccttccca
     9721 tctcatctac aaattcttga taaaactgac tggtcggaat ttctctgaca tccttatcaa
     9781 atgtcttgtc aagtgtttta ctaaccttct cagcaatcaa ttgatgctct taccatccac
     9841 tttgaaactc tgagcccgaa ctagaaacca tgactgggat aaacaacaag gtcagtagat
     9901 ttacagacaa taaggaaagt agtagacttc ctgcaaaact agaatcctag ttcatgattg
     9961 ataatgccag caatcaaatt cattcataat ccgaagcgtt tacgatgatt tcaataggtt
    10021 gttgaaaaca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt gcttctctcc
    10081 ttggatagcg catggttata ggcttgatct tcaactgtta gcggtttgag tttgctggat
    10141 ttacgtggag tttgtgcttg aggatatatc ttcatgagcc cttgataatt actgtcagcc
    10201 aagattttac cagcttgtcc aatatttccg cgactcattt tgaacaactt catatcatga
    10261 caatagttca cagcgatatc caaagaaaca attctccctt gacttgtgac aatcgcttga
    10321 ggttccatag aatgacattt ctattttacc agaataattg gctagttgat ttttttagga
    10381 cgatttattt ttacctctgt tgcatccaca atcaccgtat tctcagcact aagatgagtt
    10441 tttgaaatcg taacaccact ttgaacaaga gttacttcaa cccattggct ccgacggatt
    10501 aagttgcttt cgtggatacc aaaatcagcc gcaatttctt cataagtgcg gtattctcgc
    10561 acatatagaa agcgttgtta atttatttat ctcatttttc agaaaattct tttatttctg
    10621 taaagtctac gatactcgat gtgtttttat ataatgatac agtctgagaa tcactgttcc
    10681 actagccatt ccaatagaga ttaccaaagc caacatgaca accaaggtcg cacttgccag
    10741 tgctttatta tagtcccctg tcacaaaaaa ggcagttgtt cggtaggaga gataacctgg
    10801 aaccagcggt gccaaaatgg ccaagataaa gaccacagca ggtgtcttat aaagaatact
    10861 taaaatctgg ctgacacaag aaccaataat ggctgcaatg aaggtagcta caatgacatt
    10921 ggtcggttcc ttgagcaaga gatagattag ccagacagtc atgcccaaaa tccctccagg
    10981 taagagcata gaccgttgca cattgagtac gattaaaaaa gtgataatgg caagaaaact
    11041 tgctactgct tgtaataaaa aggttgttag tgtcatatta gttcatcaat accaaggcga
    11101 cagaagttcc tgcccctaaa gcgagggtaa tgagcaggga ttcaaacatc ttactcatac
    11161 cagagtttat gtggttggtc ataatatcac ggaccgcatt ggtcaaggca atacctggta
    11221 caaacggcat gaccgcacca gctataatca aatctgccgt tgaaggaaaa cctgtgtagc
    11281 gagcccaaaa ctgggcaatt atcccaaaga caaaggctcc agcaaaggct gtcacaaagg
    11341 gaattcggat aaatttttcc acatagaggg aaaaggcaaa accaaataag gtcgccactc
    11401 ctgccccaag tgcgtcgtag atatttccgc taaacataac tgaaaagaaa ggagcactaa
    11461 aggtcgcagc cagagttacc tgcaacttag tatagggaag gggtttggct tgcaaggccg
    11521 tcaattgctt aaaggctgtt tctaagtcaa tctgcccccc aactagctga cgagaaatct
    11581 ggttcacatc gcagactttt tcgatgttat aagaagagga ggtcacgcgc ttcatgcgcg
    11641 aaatattggt attttcaata gagaaaaaga tagcggcagg catggcaagg acattgcaat
    11701 ccacaatccc ctgcgaatgc gcgattcgaa tcatggtatc ttctacacga tggatttctg
    11761 agccactttt aaggagaata gtccccgcta gcataatcac atcaatgacg gcatttaatt
    11821 cttttgattc ttccatgctt tcctcctttt atcaactccc tctattctat cacaaatccg
    11881 gactcaaaaa aaatctttgc catgaaatca tgacaaagat tgattactca ttttgattat
    11941 ccatctgctt ttaaggagta gctgaagttg ttttaggttt gtagattgaa atcttgactc
    12001 tagtcttatt taggtctacc ttttcacctg ctctaggact ttgttcaaca accatgcctt
    12061 ctacactacc tgcaggcgct gtcgtcactt ctacaacttc tatattagct tccttaatcc
    12121 caacaatttg aatcaaattg ttcttagtaa actccaagct ggaaccaatg taactcggca
    12181 tggcaacact tgtaactttt ttagctactg tcaagacaat ttgagtaggt ttactcacat
    12241 cataagtcgt tccggcacct ggactttgtt tcataatcgt tcctggttcg ctttcgctgg
    12301 actcttcttc ctctatctta atcaaattct caggaacctt cttctgcttg agttctgaga
    12361 ttacttctgt agaattccgt ccaatatagt tccctaattg aatcgtcgta gcttttttag
    12421 ctactgtcaa aacaatttga gttgccttgc tcaagtcata ggtcgtacct tctggtagac
    12481 tttgcttcag gaccgttcca gcctcactct cattcgactc ttcttcctca attttaatca
    12541 aattatctgg aacttttttc tcttttaatt ccgcgataac atcagaagat ttccggccga
    12601 cataattact aatttggaag gattgtttgc ctgatgagac aaccagattg attttcgttc
    12661 cttcttttcg accagttcca gcgccaggat ctgtacggat aatccgccct tcttccacct
    12721 tttcactagc ctctgtcttc tcctcaccaa tctcaaaatt ggcttttttg agcgttgcct
    12781 tggcctctgc aactgtctga cctgccacat ctggaatggc aatggttgca ggagttctgg
    12841 atagtatcca aataagagaa gctgccacca atacaaggct ggccaacaaa atcaggtaac
    12901 gcatcttaaa tctatgtttt ttcggtgctt gtggttggta agtttcctct gtcacagcct
    12961 ggcttgggtt tttgattgat ttgtgttctg tctgcgcttg aaccttagga atagatgtca
    13021 aggtactctg agaaaccttc ggcaaggtct tggtatctgc cttgctcgtt tcatcaaaga
    13081 ttaacttact ttcatttcta cgattgtagg acaagctact agacaagtcc acatacatct
    13141 ctgaaaccga gcggtagcga ttggtcaact ttttagcagt tgccttgata ataacatttt
    13201 ctaaagcctg aggtacagat ggattttctg caataacgga cggcaggggt ttctggaaat
    13261 gctggagggc aatggtcacc gcgctatccc cgtcataagg gatatggcct gtcaacatct
    13321 catagaaaat aatccccatg gcatagatat cactctgcac agtcgccttc gaaccacgcg
    13381 cctgctctgg tgacaagtaa tgaactgagc ccaacatcga gttagtctgg gtcagacttg
    13441 tctctgcaaa ggctacagca atcccaaagt ctgtgacctt ggccgtccca tctggtgtca
    13501 aaaggatatt ttgaggtttc aagtccctgt gaacaattcc tcgagtatgg gccaagcgca
    13561 tagccaagag aatttgtccc atgatacgga ctgcttcttc attagaaaga ggataatgtt
    13621 ccttgatata gcgtttgagg tccagtccag ccacatactc catagctagg tactgttgac
    13681 cgtcttcctc gccaatatct gttatccgaa cgatatgagg atggtctaga tctgccatag
    13741 ctctcgcttc acgctgaaaa cgagctacag ctatcgggtc cgtctggtag ttggtcctca
    13801 gaaccttcac tgccacttct tccccatcta agattaagtc tttggctagg tagacatccg
    13861 ccatgcctcc tcgaccaatc tgtttgacaa tccgatagcg tccggcaaaa atcttgccga
    13921 tttggatcat tctgcatcct cctcgttcat agaaacaagg gcaaccgtaa tgttgtctaa
    13981 acctcctgca ttgttagcaa aacgaacaag tgtctccgtt ttatctgcta aaggaatatc
    14041 actggttaca atatcacgaa tctcactgcc tgaaatcatg ttggtcaagc cgtcactatt
    14101 gagcaagaga tagtcacctg actcaaggat aactgtccca aaatcaggct gaatttcatc
    14161 tttttgccca atagactggg tgataatatt tttttgcgga tgagcttctg cctcttctgg
    14221 tgtcaattga ccagccttga gcaattcatt aatcaaggaa tgatcgctcg tcaactgatg
    14281 gtattcttct ccacgaatca agccgatacg cgaatcacca atatgagcat agatagcctg
    14341 attatcaata atagcaagga cttccaaagt agttcccatg cctctgtaag cttcatcctg
    14401 accaagctgg tgaatctttt gattttcaat ttctaggtaa tgggcgaacc attcacgcac
    14461 ttcattgact gtatcaatct gggtatcaac ccaagctaca cccaggtctg tgaccgccat
    14521 ttcactagcg atattccctg cgcgatgacc tcccatccca tcagctaaaa taatcatggt
    14581 acgtccagct ctattgacat agtggttgac atagtcttgg ttatttgttc gtttctgacc
    14641 aacatctgtt aataatgaaa tttccatgtg tcagttcctt cctaatccga tatcttgcga
    14701 aattgactga tgaagaatcc atcacttcca tacaattcag gtgtaatgag gatacagccg
    14761 tctttcatga tatccttaca ttcatgttct agttttacct gctcgaactc gggatgactc
    14821 tctaaaaagg ccttaacgac ttgaaaattc tcctctgaga cgatagtgca ggtgctataa
    14881 gttattatac cacctttgcc tagtatttga caaacactac ctaatatttc taactgaatt
    14941 tcctgcaagg acgcgaaatc tgccgtttct ttattgtatt tgatatctgg ttttcggcgc
    15001 aaaagaccga ttcctgaaca aggagcatcc accaaaatct tatcaaagga atcctggtca
    15061 aaaaactcat gcacctttct ggcatccaat ttttgagttt gaacccgatc tgcaactccc
    15121 agacgttggg cattttcttg aattaaatcc aacttgtggt cgtacaagtc cagagcagta
    15181 acctgacctg tcgtaagata agaggctata tgggctgttt tcccacctgg agccgcacag
    15241 gcatcaagca ctcgctcatc accttgtaaa tcaagcgtcg gagcaaccag ctgactggac
    15301 tcgtcttgga tggtaatggc tccatccgca aacaaattat gccctgcaaa atgcccctgc
    15361 tccttaacca gaccagtggt tgctaaaagg gaatcattcg cctccaacaa ggcttggatt
    15421 tcctcttttc gacttaggtc tgttacacga atactggctt tgtttcgcac taacaggctt
    15481 tcaaagatgg cttttgctct ctcctctccg tattcttcct tgagtttggc aactagccaa
    15541 actgggagag aataggcaat ggagtcacgc ttgttttttc gcttgatgct agcaatatct
    15601 ggccagcctt cacgcaagat acggcgaagg acagcgttga ccaatttttc actgcctttt
    15661 ttacggagtt tggctaattc cactgcttca ttgaccacag catgatctgg aatcttgtcc
    15721 aaatagcgga gttggtaggc actcatgaga agaaggacat agagccagct gtctaactgg
    15781 tctctgtctt cgataaagtg ggataggtac cattccagag tcagtttacg ggctaccgtt
    15841 ccatagacta gctcggtcac taagcccttg tctgctgcag agagctgact ccccttgagg
    15901 tgtttattta aggcaatatt tgaatatgct tgattcacaa aaacatcctc tagcactgct
    15961 agagctaaac ttctagccgt ttctacttta gtcaccaaat cgttctccta cagtcaatgt
    16021 acgtccaact ccattgagga aggaagcaat gtccatctta ggcttaccag ctggctgcac
    16081 ttgtttgagg gatagagccc cttcagccgt tgcgacaatc aattctttct tgccgataga
    16141 gagaatctca cctggatttc cctgaccttc tactggtagg acttcataaa tcttaaagcg
    16201 gtcgccctta aggaaagtat gggcaacagg ccaggggttc attccacgaa tttggttaaa
    16261 gagttgacga ttggttttgt tccagtccag tttttcttcc tttggcttta tatttggaga
    16321 gaaggtaacc tgactcgtat cctgcggttc aggtttgata tcaccagcaa tataggcagg
    16381 cagagtgtcc aaaagcaaat cacaaccaac tagcgccaat ttttcaaaca aggtgccaac
    16441 attgtcctca tctgtaatcg gaatgctgcg acgagaaatc atatctcctg catccatttc
    16501 cttaaccatt tccatgatgg tcacaccagc ttcctcatcc ccttgaatca aggcataatg
    16561 gataggcgca ccaccacggt gtctaggaag gagggaggca tgaacgttga cagcaaagtc
    16621 catgctatca aggagtttgc ttgggagaaa ctgcccaaaa gcagcagtca caattccatc
    16681 tgctcctagc ttcataagat cttccatctc tggacttcca gataattttt caggttggta
    16741 gatagatagt cctgcttcct tggcagcctg cttgactggg gtttcttgga taactttttt
    16801 acgaccaaca gcacggtctg gctgggtcac aacggctaga atttcgtaac ggtcatctgt
    16861 caaaagtcct tttaagactg ttgctgaaaa gtcgggggtc cccataaaga ttagttttgt
    16921 catatcttct ccttcttata aaaattgctg cggctcatgg tcaatgctga gacggagctc
    16981 actattttcc cgttcttgag tcaaggccag gacctggttg agggtcggcc ccagctcatc
    17041 ttctaaacgg tatttaatta aaatctggta atgatagagg ttgtgggtac gggcaatcgg
    17101 ttttggcgtt ggccccagaa tgggactggt ctctgacaag cctgaccgca aaatgttcat
    17161 gacttcatag gcacgtttga aaacctcttc ttctttcttg tgagaaaggg taataccaat
    17221 cgtgaaatag taaggtggat agccgagttg tcgtctgatt cccatttcat aggcataaaa
    17281 gccttcgtaa tcttgatcct tggcaaatcg aatagcatag tgctgcggat tgtaggactg
    17341 tatcaagact tgacctgctt tttcagcccg acctgctcga cctgccacct gagtcaagag
    17401 ctggaaggtt ctctcagaag aacggaaatc aggcagattc aaggccgtat ccgcatttag
    17461 aactccgact agggtaacat tgggaaaatc caaacccttt gcaatcatct gagtaccaag
    17521 taaaatatcc gcttcccctc gcccaaactg gtcaagcaag gcttggtgac tgcctttctt
    17581 tcgagtcgta tccacatcca tcctcagaat gcgagcttgg ggaaagagtt ctgctagctc
    17641 atcataagcc ttctgagttc ccgtcccata gtaacgaata ctgcggctct tacagttagg
    17701 acagacctga ggaatatcct tcgagaaacc acaataatgg cagttcatag tcttggtatc
    17761 catatgcaag gtcagagaaa tatcgcagtt gggacaagta tccaccgtcc cacactcccg
    17821 acacatgaca aagctagaat aaccacgacg attgagcatg agaaccacct gctctttttt
    17881 aaccagacgg tcttggatag cctctagcaa aggaggcgta aagtttgacg tctcattttg
    17941 tccgatatag tctcgaaagt caatcacttg aacctcaggg attgtagcca aaggattggc
    18001 acgttgggtt agacgtaagt gttgatagac gcctttgcca gcacgcgccc ggctctctaa
    18061 gctcggcgtt gcagatccaa gtaccagagt tgcttgatta tactgagccc gtaaaatagc
    18121 tacctctctg gcatggtaac ggggattgct gtcctgctta taagccactt catgctcttc
    18181 atcaataatc atgacaccca gatttttcag aggagcaaag atagcagatc tggcaccaac
    18241 aacaacttgg gcatcgccac gctccacctt gcgccattca tcatactttt caccattgga
    18301 taatcctgag tgaagaatgg ctaccttgtc cccaaaacgt gctataaaac gctcggtcat
    18361 ctgaggagtc aaggaaatct caggtaccag caaaatagct gtcttgccct tatccagggc
    18421 accttggata atctgcaagt aaacctcggt cttcccactt cctgtaatcc cttgaagtag
    18481 aaagggaggt tgagaactgc caatagaact cacaaccgca tcacgcgcct gtctttgttc
    18541 tggatttaac tccaaaggtc tacttgcttc aattccttca aaataagcag ccgagcgttg
    18601 aacttccttt tggactatgg taacagcacc ttgatccaca aagaagttga cttgctctcg
    18661 cgagtaggac tctaacaagc tagccaagga agcgctctct ggatgagaca gcagataatc
    18721 tctcagttcc aactttttct tggcacgtgt agaaatctca acaccttcta attgagcatg
    18781 gtcaacctca taccaagact gggtcttgac cttcttttga tcgactgcct gatattccag
    18841 accaagcagg ccttttctag tcaaacgcat catttcagct tgcttggcaa ggtctagtga
    18901 agaaaaggct agcgaatctt ctgaaccaaa caggcgcact cgttcttcct gactcaagcc
    18961 ttccagagga tagagaatct tgtcatagct agaattcaga aatcctggaa gcatggcctt
    19021 gaggatagaa attttgtagg agaagacaga tttgcgtaac tcctcagcca gccagagttg
    19081 ttctggcgtg agaacaggag aaaaatccag cacctctgca atatctttta aatcttgctc
    19141 catctcttct ccatctgatt gggacttcaa accaagaaca atcccttgaa tcaggcgatt
    19201 acccttacca aaaggcacat gaacccgcat cccaacttcc agcattccct caaattcctc
    19261 cggaatcctg taactatagg gctggtccgt ctgcatcaag ggcacatcta cgataatctt
    19321 agctagggcc atcttctcac ctcctccttg tcagtacatt cttgcaatag aaaaaataag
    19381 attgagtccc cccaacctta aattttttca ccatcttctt tttctttagc aatttgctct
    19441 ttgattttct tttcttcttc ttctttgcgg cgtttttctt cttcgatacg gcgacgcact
    19501 gcttcacgtt ttccttctgg atctgggtga attgtaacgt ttcctgattc gatttcttct
    19561 aaagcgcgaa gagttgattt ttcagacttg aaaccttgag ttgctggggc acctgcttcc
    19621 aattcgtggg cacgttttgc ttccaagatt acgagtgaat attttgaagg aaccttgtcg
    19681 agcaaggtat caatagaggg ttttaacatc atttgcttgt acctattttc taaattttat
    19741 cgggtagttg gagattttgg taacatctcc tgatagtgac caatgacacg atccacacag
    19801 aagtgttctg cttcaatcac acatttgaca cgttcagcag ctagggacac ctgatcgttg
    19861 acaatggcat aatcatactc acgcatgagg gcaatttctt ccttggcctt ttcgattcgt
    19921 tgggcaatca cttctgcact atctgttcca cgacctacca agcgatcttg caattcatcc
    19981 aaatctggtg gtgtcaggaa gataaagaca gcatctggaa cctttttctt gacctgaaga
    20041 gcaccctgaa cttcaatttc aaggaaaaca tcgattccct tgtccaaggt ttcattgaca
    20101 taggtcagag gagttccata gtagttgccg acatattctg cgtattccaa catctgtcct
    20161 tgacgaatca gctcttcaaa ttcttcacga gtacggaaga aatagtcaac accgtccact
    20221 tctccaggac gttgtgcgcg tgtcgtcatc gatacagaat attgaaattg gttttcagaa
    20281 ctctcaaaaa tctctcttct aaccgttcct tttccaaccc ctgaaggacc agaaaaaacg
    20341 attagtaagc ctcggtctgc cattgtgtct ccttttagtc aatctgtgaa ataacatttc
    20401 tctagaataa tggcaaaaag ccagattatc ctttacagtc tttctatcta gtgtaacaaa
    20461 aaagcagtaa tttttcaact gctctttctt atttatttag cataatctac tgcacgaagc
    20521 tcgcgaatca cggttacctt gatatttcct ggataatcga gattgttttc aattttctta
    20581 cgaactttgt gagccaagat tgtgactttg tcgtccttga tttttcctgg attgaccatg
    20641 atacgaattt cacgtcctgc ttgaagggca aagctagttt gcactccttc aaagccgtta
    20701 gcaatttctt ccaaatcatg gagacgcttg atgtagcttt caagagactc actacgagca
    20761 cctggacggg ctgcgctcaa ggcatctgct gcagcgacga taactgctat cacgctctca
    20821 gcttcaacat ctccgtggtg actagcaatc gtattcacca caactggggg ttccttgtac
    20881 ttacgggcca attccatacc gatttcaacg tggctacctt caacctcatg gtcaatggct
    20941 ttcccgatat cgtgaaggaa tccagcacga cgggcaagag ccgcattttc accaagttcg
    21001 ctcgccatga taccagccaa cttagcaacc tcaatcgaat ggcgcaaaac attttgtcca
    21061 tatgaagtac ggaactgcaa acgtcccata atcttcatca agtctggatg aaggtttggc
    21121 gcaccaattt cataggcagc agcctcaccg tattcacgaa tcttattgtc aatctcttga
    21181 cggtttttct caaccaactc ttcgatacga gctggatgta tacgaccatc tttgagcaac
    21241 atttccatag tcatacgggc aatctcacga cgaatcggat caaatcctga caaggtcacc
    21301 acttctggtg tatcgtcgat aatcacatcg acccctgtca aactttcaaa ggtacgaatg
    21361 ttacgacctt cacgaccaat aatgcgtccc ttcatagtat cgtctggcag atgaactgtt
    21421 gagtttgttg actccgctac atattcacca gcgatacgtt gcatagcttg aaccaagatg
    21481 tccttggcca ttttgtcaga acgttccttg acctcttgct cagcttcgcg aatgcgactg
    21541 gcaatctccc tggtcaagtt ttcctctgtc tgagccaaga taatatctcg tgcttctgcc
    21601 tgagacagag caccaatacg ctctagttct gcttcttttt gtctttcgac ttcctctaat
    21661 tgctcttcac gcgcatcaag gtttttcgct ctatcagaaa tactttgttc tttttgttca
    21721 agtgtttgtt ctttactcgt caaattgtcg tccttacggt caaggctagt agctctctct
    21781 gtcaaacgac tttcgatttg tttgagttct tgacgttctg atttgaattc agcgtccact
    21841 tcttcacggt attttctggc ttcttctttg gcctccaata gtgcttcttt tttaagagac
    21901 ttgctttcac gtttggcttc attaacaagt aaatccgctt cacgctcagc ttgtccacgt
    21961 aaattagttg cttcttgttc agcatttaaa agcatcaact ctgcagcttc ctgagatgat
    22021 ttcatcttag ctgagatgct gacatatcca atgactaaac caatgatgac ggcaaaaaca
    22081 gcaatcgcaa gcgacatgat ttccatgttt ttacctcatt ttattgttat tccgaatgac
    22141 atacattctt ttacattcta ccataaaaaa gtgattttca caaacctaaa atagaatatg
    22201 ttttggggaa tttggaacac atttgcaaaa ataaacttgt tgtttagaaa tagtagttta
    22261 gtagagactt gagaaaaagc ctactttgca gtagacttag taatgatctt taaaggacaa
    22321 gaaagccacg ctatctccat ccatcatata aatcaagcga ttttctgcat caatacgccg
    22381 tgaccaggct ccttggtaat catatttgag tggctctggt tttcctattc ctgtaaatgg
    22441 atcgcgttga atatccttga ttaatttatt gattcttttt agtgtttttt tatcttggtt
    22501 ttgccagtag caataatctg cccaggcatc ttctgtaaac ttgagtagca ttcatcactc
    22561 ctcaatcaca tggacctgag tgcttccagc acggacttga gccattcctc gcaaaacctt
    22621 gtctgataac tccttatttt gggcgattct cagggtttcc tgaatactgt cccattcgct
    22681 ctttgaaaga actacaatgt cctcatctgg atttttattg accaccgtca aaggctcaaa
    22741 ttcatcattt accttcttca tgtagtcctt taaatgattt cggaatgttg agtaaaggac
    22801 tgcttccata accatacctc gttttagctc ttttccacta ttatacacga aaagaaagaa
    22861 attgtcagga acttgtacaa gattttcttt tctatctatt tatactcaat gaaaatcaaa
    22921 gagcaaacta ggaaactagc cgcaggctgt acttgagtac ggcaaggcga cgttgacgcg
    22981 atttgaattt gattttcgaa gagtattatt cgtaaaaaat ctcaaaaagc ctacctttcg
    23041 gtagacttag tttgtttcta ttctaatcgg cactcttcca aaattttgct ctgctatact
    23101 tggctttcct agttggtaaa tctggtctgc tttttgggtc atagcatccc aaggttcttt
    23161 gccaattcgg ctgactagac tgacctgacc tttcaaagac ttgagatgtt gcctgccttt
    23221 ttcagtaaat ccaaggacat gaatcgcttc tggcaagtta ctttctctaa cctgcaccaa
    23281 aatataggtc aataggcgtc tgacacgcgc cttggtgtaa cgtttggtag tcactaactc
    23341 gaccaattct tccacagact gggctgtttt aatagcttct ttaatgcgca cagccatttc
    23401 ttgattgacc tgatagatgg tggttaggtc gggatttgac aagatttgat agcggagcaa
    23461 gggaaaatag tcttcccagc tcaccttact ggcctgctca aagagggcaa cagaaggcat
    23521 aaatcgttct aagaaatctt tgtccctctg gtgctgacga agggctgtcg ccgaagcaaa
    23581 gttcacatcc ttgtccacag aatggtagcc tgccccctga cgctgaatcg gatgaagttt
    23641 gatgttgtac ccagcaaccg ccttggcgta agcgagggca agaacatgat tgggcgtatt
    23701 gcctgaaaaa tcaagacctg caaattcctt ccacatggct tgggtcttct ggggataaga
    23761 aagagagtca ggcaaatttt ccacaaaatt ctccatctca gccccctgct ctgtgtataa
    23821 gtcagcgatt ttttgataat ctagaacttc ttctgtccca aaagcgaggc tatcaatgcc
    23881 caaccaagtc aagatatcca cagctccttg accaaagaaa tcagctgcct gaacactgac
    23941 taaaaagggc aattccacta ccaagtctgc gccattttcc agcgccatct gggcccttgt
    24001 ccacttatcc acaatagcag gctctccacg ctgcatgaaa ttccctgaca tggcaacaat
    24061 tttcagtccc tctgcctgat ccagcaggta tttatggcca ttatgaaaag gattgaactc
    24121 cgcgataata cctgtgatgg tcatgctaac ctcctacttc tgcgccacaa aaaaccaacg
    24181 ggtgctagtt tctgttggct ccttatcctc aaagtctgca tagagtttga aggacttgaa
    24241 tccagcctgt tccagtaaga tatcataggt caagatctca taggtcctct cctcatgtac
    24301 ttcatcgtgg cgactaaagg aaccgtcagc ctccttgata aagaaagtca gctcatgcac
    24361 gatggagtga ggagtttcgt cctcatatgt atcccaaagc atggcaaaat cttccgcatt
    24421 ttcatggtag gaatagccag ggaatacttc atctgtctgg taggtcgagt gcacgtcaaa
    24481 gatgaaaact ccctcttcat tcagcgcatt gtacacgtcc ttaaagacgt cccctacttc
    24541 cacctcatcc tgcatatagc agatagagtc cgaataacac gtgacaaaat cgtatttccc
    24601 tgccttggac aaattcagca tattaccttc tataaaagca atcttttgct tggctgaagt
    24661 tgctctcttc tccgcaatct tcaacatatc cgcactcaag tcaagtccag tcacatcaaa
    24721 accagcctga gagaagcgca ctgattgaat tcctgtccca caagccaatt ccaagagttt
    24781 ctttctctcc ttggtcttag gcaaatgacg cagagaaaaa ttcgtccatt tgtcgtataa
    24841 actatcgtcc atgaccgcat catagaccgc cgcaaaggtt tcataagtcg ccataatcat
    24901 gatactaaga gctccatggt aaacaccact agccctttac ctttctatca atttttctaa
    24961 aataagcaaa gaaacccagt tgactacaac tgagttcatc ttctaagcaa gagcttctga
    25021 aatatctact gaattcgcct catgccatag cttctctaga ttataatggg cacgcatttc
    25081 ttctgaaaag atatgcacga caacagcacc gaggtccagt aagacccagc ctccagctgc
    25141 atcgccttcg acatggctac ctttaaagcc tgcttgagct actttttcac ggatattagc
    25201 tgcgatagcg tccaactgac ggctattcat tgagctagtg atgacaaagt agtccgtcac
    25261 actagtcaaa tcttgtacat caagtgcgag gatatcctcc gcacgtttct catcagccgc
    25321 tttcacgact agttctagta attctttttc gttcatttaa tcctcttttt agaaaatatg
    25381 tttgtaatct agaacatctg caaagcgctc ctcccaaatg tcctcataaa attcattaat
    25441 cgtttctgct ggaatgtcat ccccatcaaa ggttgtgaca gcaccttctg gaacaataag
    25501 ctgatagcca tattcaaagg caaccttgac agaggtatcc acacaatatt ctgtctgcat
    25561 accacataaa actaattttt caatcccctg cttatccaag tattctttta agccagtttc
    25621 tttgaaaata ctgttatact tcttctgaaa gaccttttca gcaggttttc gatttaaaag
    25681 cgcagataac tgccaatctt ctgatgtttg agcttcagag ttctcaatat gttgaacata
    25741 gataatttca atattcttgc ttctagcctg gttttgtaaa taagaaattt tttccaatag
    25801 actttttgtc tggaacccag tttctactaa aacattctga acatcaatga ttataaaaga
    25861 cgtcttcatt tagtcctctt tcaaatagtg cacaaaggcg ttataggttt caagggtttg
    25921 gggatagatg gggaatccct gatgagctag atactccacg gtacgagctg tttcgtaggc
    25981 caccgcctta ttgagcgata gacttgcaat ctcacgcgcc acatccactc caggaaatgc
    26041 ccgattgtgc tcgatatagt ctgcgacgta gatgacctta tctaggtctg tcatttgacc
    26101 agctccgact gtatgaattt caatggctcg cagaatttca gaatcatgca aatccaaatc
    26161 ttcctgaatt ttgtaaattc cgaccatacc atgccagaca ttattacccc agtttttgag
    26221 gtcgaaatct agctgataac ggtcaatcaa gtctagaaat tcctgatctg acaacttttt
    26281 agcatagtca tgaagaagac ctgctagact tgctttctca acatctacac caaagcgctg
    26341 agctaattcc atagcggcac gctccacacc tagacaatgg gttagacgtt tctcaggtag
    26401 aagctctgcc attttttcca acaaggcctc acgggagcag ttgatatagt cttgatagga
    26461 catcagtaga gcccctcctt ctcgatgtag tctagcactg gctgaggtag gagaaagttg
    26521 ggtttccgac cttgggcaat gaagtcccgc accatgctgg acgagatatc catgagcggt
    26581 acgtccaccc agataactgg ataggaagtc cctgccttgt agcgtggacg ctgaaccccc
    26641 acaaactgaa ccatgtcaac cagttcatca attcggtacc acttaggcag atagtcaacc
    26701 atgtcggcac cgatgataaa gtaataatcc gtatctggat tcttctctgt caaaatcttc
    26761 atggtgtcgt aggtgtagga aatacccttg cgctccaact caatggtttc aatgactagg
    26821 ccgtcaattc cctcaattgc caactcaagc atcttgagac gatggtgttc agggatggtt
    26881 tcctttttat caacgtgagg aggttggtat tcaggcatga gaagaacttg atccagtccc
    26941 aactgttgcc gtacttgatc cgcaacaatg agatgggcat tgtgaacagg gttaaaattc
    27001 ccccctaaaa tcccaacttg tttgcgtttt ttctccttga tttctggctc caactctacc
    27061 ttggtaaagg gagtcaatag ttcgattgtc ataggctagt cccctttatt tctctataaa
    27121 aacagttatt tggagtttcg gtcttagatt tctttgactt tcttagaaat cttgcgattt
    27181 tctttcttgc tagattgttt aaacaaaatc aagatgcgtc ctattttttg gactgtatcc
    27241 acaccgattt cttcttccaa aatttcagct acttcgtgga tgttttcatc tgtgttttgt
    27301 aagagagtaa ccttgattaa ttcacgcgca tcaagagctt gacggacgct ggttttgatt
    27361 tggtcgttga gtccattttt cccgatttgg atgataggtt tgagggtgtg tgcctggctg
    27421 ttgaggaagg cacgttgttt tgatgttaat gacataattt tcttccttgt tttttgatag
    27481 ttattttagg tggtgaggga cggtcggaac taattttcca aagatttcat ctttgaaaaa
    27541 tggatttcct gacctcacaa ttgagccttg gtctcaattg tgccccttgc caatctacag
    27601 attggcaatc acaccacggc aataactatc gctatatgag ctcactttgt tcgctcagcg
    27661 attttgtttt aaataattgc ttttcgtgtg acgacagcga cgccttctgg tgcccagacg
    27721 gcgacttttg ctatgcctgt tacacgaatc catcctaggc ctgagataac taggtctgtc
    27781 ttatctttaa tggtaaagac atgctggact agccttggga aatcttcttt ttccttgcta
    27841 tttggtggtg tcagaagagt tcccaggtgc ttatcgtaga aagcactagc tccttcaagc
    27901 ttgctacgat ggagtttgag ttcattatca aagaaagcag taaatccttg cttttctcct
    27961 gctataaagt caaagcgtcc caaaccacct aaaaataggg tttgctcagg attaagctga
    28021 taggtcttag gcttgatttc ctttttagga ctgacatact tgaggttttt ggccgtcaag
    28081 tagtgagcca tctggtggcg gtggataatt cccggcgtat cgtaaatata agatccgtcg
    28141 tcaagcggaa tctctatttt gtccaaggtt gtccctggga agcgtgaagt agtgatgaca
    28201 ttctgatcac ccgtgatttc ttggataata gcattgatta gagttgattt tccaacgttg
    28261 gtcacaccga ccacatagac atcgcggccc ttacggtagt gttcaatctt atcaatgact
    28321 tccttaatgg catgtttatt ttgtgctgaa gttaggacca catcgactgg acgaagacct
    28381 tcttcacggg cacgtttcat gagccactgg ctaatcttac cagacttaac tgacttagga
    28441 aggatatctt ttttatttcc taccaagagg acatcattgc ccgagacgaa acgtggtaaa
    28501 cctgggatga cagatccatt aaaatcaaag atatcaatga cattgaccac taaggcatca
    28561 ctgtctccca cctcgtgcaa gagcttgagg aaatcatcgt ccgtcaactg gacatctgtg
    28621 atttcattgt agtggcggag acggaaacag cgttggcaat agacttcgcc agtctccaaa
    28681 cctttttcaa gtgccgactg gggggtaaaa ccaagaccag ccttatctgt cgtctgaatg
    28741 gttgctccac aaccaataca gagaatttct tccatagtta aattcctttt ttatatgtaa
    28801 tcggtccgta cttttcagtg atttttctca taacacgacg ctcacgagtt cggttaatct
    28861 gcgttttgat tgagtcatgt tggaccaagg gtttgactaa aattgaccga atccctgcac
    28921 ggtgggctgc tcgtatatct gtcatgagct ggtcgccaac catgaccact tcctttttgt
    28981 catagtggaa ttccttcata gcacggtcaa taccaaatgt gaagggcttc aaggcccagt
    29041 aaacgtaatc aatcccaaat ttctcaactg ctcgttgaac gcgttttttg gtgttatttg
    29101 acactacgat aatgccaata cccgcgtccc gaaggtcatg tagccattgc ttcatctctg
    29161 gcgttccatc agggttgttc caagcaatga gggtattatc caaatcgacc aaaacagcct
    29221 tgattccctg cgcctgcagg cttgggactg tcagatcata gactgcttcc acagcaaaat
    29281 ctggtatata attttcaatc gccattctgg ctccttttct taactttttt tcgcaatttt
    29341 ccttagtaac aaggataaaa caatccacaa acctgcacta gccaaactga tatagagcca
    29401 agcatggggc tcatctgtta aaggtaaggg aacattcatt ccgaaaaagc ctgtcacgac
    29461 tgccaaaaca gctagcaaga ctgaaatgat agtcaaggtt gtcaaattgt cattcagatt
    29521 attgtttaga atattgttgt aagaggctga aagctgctgt aaaatctgag agattaggtc
    29581 tgtcatggat accagctgat gagcctcaat catggcatca tcaaactgtt ctctctcaat
    29641 ctcatcaaaa ctacgataca aggcatgacc ttgaatatgc tctaacaaaa tccgattttg
    29701 tttggcagct gccgtcagat aaaccatacc agtctccaaa tcagacagga caaagaggtt
    29761 tttcttggta gttcgctggc gcaagaggtc attgacctca tccctactct tgtccatctg
    29821 ctcaatgaca ggatagtagg cattgctgat aatttccaga ctggcaaaga gaaacttata
    29881 aatcgaaagc gtgtcatggt tctccagata acgagtcatc tgttcaatga cataggcgtt
    29941 cttggtatta ctaatggtaa tcaggcgacg atgctcgaca ataaaggtca tgggaaaagt
    30001 ctcatagtag gccttgtcct tttttacgtc taagacatta tagataaagg taaccgtccc
    30061 actttcacgg tggtagtcca tgtgggcgcg ctcgtttcta tccagtgcgt attcaatggt
    30121 ttccttgtcc aaaccgtaaa tttcagaaag gtcttctagt ttattcaact ttcctaggtc
    30181 taggtctatc caggtacaac cattgcccaa ctgcttttct aaaaccatct tttctcctta
    30241 ttaaactccc tctattgtac cacaaattac ttaaaataac aggtctagtc tgtgcgtgag
    30301 aaattgctaa caactgtgat atttcggtta gggacaaagt gaagggcttg ctcctcgcta
    30361 cgaagctgtg tcgtacgaat tcccacactg acaaccttac ccgatacagt aatcggtcca
    30421 tttgtcagaa cgacctcatc tcccacatcc agttgacgtt caaagaggat gaaaaagcca
    30481 ttgatgacat cagatagaaa gccttgggct cccataccaa tcgctacccc agcaatacca
    30541 gctccagcca gcaaactaga aactggcaaa cctaaaatcg acaaaatgca gtagagtaaa
    30601 aagaaatata gcgtataatt aaacacattt tctagtaaac gtgagatggt tttttggcgt
    30661 ccaacatcat gacgagacat ttttagagaa ggtttgacaa ttctctgcac catggtatga
    30721 agcatttttt tagcaatata aaatacaatc aaaagcagta aaagagaaat gaccttggtt
    30781 agaatattct cgataatggt tgtcacatct agcttttcaa tataagcctg aataaatttt
    30841 tgcatataaa actcctttat ttcattatac catattttca tcagtctcat tctccataaa
    30901 tattcaaaaa atagacagaa aattcttgat tttgtctatc atttatacta taatcataat
    30961 cactacacaa aaaggagatt attatgaaaa gaatcattca tgcttggaat aaggcaagcc
    31021 ttgtcaagcg tatcttgatt ggtatgattt atggggaatc ctaggactga cccttcctaa
    31081 tctctcaggg attgggctac tcggggattt attcgttgga ggtctaaaag ctgttgctcc
    31141 tatcctagtc tttgccctcg ttgccaatgt cctttcccaa catcaaaagg gacaagatag
    31201 caatatgaaa actgttgttt tcttgtatat cctatgatag ggactttcgc cgctgctctt
    31261 gtagctgtac tagcaagttt catcgtccct attgaaatta ccctaaatag tgccaatact
    31321 gaaattgcac caccagatgg gattgggcag gtcctcagca acctcttgct caaactggtt
    31381 gacagcccag tcaacgccct gcttactgct aactatattg gaatcttatc ttgggcagtc
    31441 atttttggaa tcgctatgag agaagccagt aaaaccagta aaaatagtaa agaattgcta
    31501 aaaactatcg ctgacgtgac ttctaaaatt gtcgaatgga tcatcaatct ggctccattt
    31561 ggaatccttg gtcttgtttt taaaaccatt tctgacaagg gagtcggaag ccttgccaac
    31621 tacggtattt tattggttcc attagtaacg actatgcttt ttgttgcccc tgtggtcaac
    31681 cctttgattg ccttcttctt tatgagacgc aatccttact ctctagtttg gaactgcctc
    31741 cgtgtcagcg gtgtgacagc ctttttcact cgtagttctg cgactaacat tcctgtcaac
    31801 atgaaactct gccatgacct tggactcaac ccagatacct attctgtttc tatcccactc
    31861 ggttctacta tcaatatggc tggagtagcg attaccatta acgttttgac ccttgttaca
    31921 gttaacactc ttggaattcc tgttgacttt gccacagcct ttgtcctcag tgtggtagca
    31981 gctatctcag cctgtggtgc ttcaggtatt gccggaggtt ccctccttct tatcccagtt
    32041 gcttgtagcc ttttcggtat ttctaacgat attgccatac aagttgttgg ggttggtttt
    32101 gtgattggtg tcatctaaga atcatgtgaa acagccctta actcttctac agatgtcctc
    32161 tttaccgccg ttgccgaata cgcagcaacc cgtaaaaaat aactcatcaa ggcaagcctg
    32221 ctcatgtctt gtcttttacg cttttattct aacttattag gaaattctta tgtctattag
    32281 ccaacgtacg accaagctca tcttagctac ctgtcttgcc tgcctgcttg cttattttct
    32341 caatctttcg tcagcagttt cggctggaat tatcgctctc ttgagcctat ctgatacgcg
    32401 tagaagtact ttaaaactgg ctcgcaatcg tcttttttct atgcttctag ctctggctat
    32461 cggtgttcta gcttttcact tgagcggatt tcatatctgg agtctcggcc tctatctggc
    32521 cttctacgtt cctttagcct acaagatggg ctgggaaatt ggcatcacac caagcactgt
    32581 tttggttagc catctcttgg ttcaagagtc aacctctcca gaccttctag tcaatgaatt
    32641 ccttctcttt gctattggta caggatttgc cttgcttgtt aatctctata tgccttcacg
    32701 agaagaggaa atccagcact accacacgct ggtggaagaa aagttaaaag atatcctcca
    32761 gcgcttcaaa tactatttat ccagaggaga cggacgcaac cgagcacagc tggtagcaga
    32821 attagacacg cttttgaaag aagccctcag actggtctat ttggatcact ctgaccacct
    32881 ctttcaccag accgactacc atatccacta ctttgagatg agacaacgac aaagtcgtat
    32941 cctgagaaac atggcccaac agattaacac ttgtcacctt gccgccagtg aaagcctgat
    33001 cttagcgcaa ctcttttcaa aaattgcagg tcaactgagc cagaccaatc ctgcttctga
    33061 tttgctagat gaaattgaac gttatctgga agtcttccgg aaccgcagtc tgcccaagac
    33121 aagagaagaa tttgaaaccc gcgccaccct tcttcaactc ctacgtgaag ccaaaacctt
    33181 catccaagta aaagttgatt tttaccaaaa atatagacag taatactctt caaactacat
    33241 cagcttcacc ttgctgtata tatgttactg acttcgtcag ttctatccac aacctcaaaa
    33301 cggtgttttg agctgacttc gtcagttcta tctacaactt caaaacggtg ttttgagctg
    33361 acttcgtcag ttccatccac aacctcaaaa cggtgttttg agctgacttc gtcagttcta
    33421 tctacaacct caaaacggtg ttttgagctg acttcgtcag ttccatccac aaccttaaaa
    33481 cagtgttttg agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    33541 aaaaaagaaa acttcagacc aatccacaaa aggtgaatat ctgaagtttt cttttattta
    33601 taagtaatca ccataaaggt taatagtaga ataaataaag ccaaactcac caataaagtg
    33661 agcaccttga ccaaggtgtt gctctgccag tagcttggtt taccaatatt actagtggca
    33721 tccatggaaa atagttgatt ttgacggggt tgtatagtta ccaatacaat caaagcaaga
    33781 gataggataa tagctaaaac aattagtatt tcaatcatag atctacctca aaattcccag
    33841 caaagtaaag agcaaaccaa tcagaatcat taagcccagt aaaagtgaaa aagttttact
    33901 aatcttctcc cctcgggttt ctttttcctt cttgatgggt ttttgtttca actcttccat
    33961 gcggccttca acgtctttgt aaaataaatc ttcctctgac atgttagcct cctaaaacag
    34021 tttgcatggt ttcctgatat ctcaccaagt caacataatt tgcatgttgg ccttgtttgc
    34081 caagtgcctg acgcatttga tcaacatctg aaagggccaa tttaatggtc tcaaccaaag
    34141 cagcaacttc actactttca aatagatggt ctggagcgat ataaagtcta ttgtgcaccg
    34201 tctgattaaa gccaagaatc aagagattgt gctcaaaggc ctgacgcact gcctgcaata
    34261 actcattact gtggtttata tccaagtaaa tatccgacag ttgatacagc tcctgaatct
    34321 tctgtggact agcattctgg taaagggcca cattaggata gcaaagcatg tctaagagct
    34381 tagaagacat ctctgtcact gctgcgatac ggaaggtaac atcaggcaag gcttctacga
    34441 ttgcttctac ttgctcaatc tgatctgaat tggtcaagat taaggcgtct cgtcttagga
    34501 aattatcacg tttgaactgg taatgataac ccaagtgcac aaactgatca tggtatttct
    34561 cgtcagttaa ctctaaagcg cgctcatatg tcgtcttatt tggaatgatg atcttcttag
    34621 tacgcacatt atcactttcc aaaatcaact gcatattacc tggaatggca tcataaagag
    34681 gttcctgcca taccaagaca tccgagccag atttatctgg ataatgaaag gaaaccaaga
    34741 aaggagtcgc tagagtatta aagataagct gactggtgtc tatttccaaa tcttgcaaaa
    34801 agaaggtgat aaattcaact ttatttgcaa agtaacgcat ggactgacca ggcaaagtca
    34861 ataagatatc acccgtcaca tggttttcca gtaaaacttg ttgaccattg acatcttggt
    34921 aaactgtcat aatcggctcg ctatctgcgc tataagtggt tttagcaaaa caagttccga
    34981 aacgattata gtggtcaacc tgacgtactc gaccttctag gtctttccag tctacctgtt
    35041 taaccaagcg agcctgcatt ccatcagcat aatgaatgac agccctctcc tgcgtcacat
    35101 cttcaataca agcagactga ttatctccta aaatttccca aaaatctgaa acgggaactt
    35161 gattaaaata gagaggtttt ccatcctcgt aacctagata ataggtaaaa ggagaaagca
    35221 gaccatcagg cagaaaacca tctgcatcga tgaccactcc aagttgagaa agaccagtag
    35281 cgactagact ttcatgtaaa tctcgacttt catgactata acgatcataa agttcaatca
    35341 tggagcacct cctctactgt tttcttccac ttttctaaaa tttctttggt caagaagcct
    35401 tctgcaattt ggtaagaata ggcacgcata gcttccaaac gattttcttg atacaattga
    35461 caaatcttag cggcataagc ttgcttgatt tggtcttcta catggtcaga tgaacttgga
    35521 atcaaataac cattttgccc atcctctata aaggtctgat taccataagg cacatcaaaa
    35581 ccaattagag gtagacctga accaatagct tccatcaagg tcagaccaaa tccttcgctg
    35641 gtagaagccg ttaagtagac ctcatactgg ctataaatct gcgaaagttc cgcatgcccc
    35701 ttgagttgga tatagtcctc tgcctgatga tttgcaataa tgtctctaag cagagaatct
    35761 tctccaccac taccatagat atcaaaggtt agttccggta actccttatg agcttcaatc
    35821 acagctttca caagccaatc aatgtgcttt tctttggcaa gacgtgaagc cgtaatcaat
    35881 gaaaatggtt tgcgcccttg acttgaatct gtcaaggaat caatactgcc tacaggaatg
    35941 gtaacaatct ttggctgatg ctgagtatat ttggcaaatt gctcttgtag aacttcattt
    36001 tgtctatcag tagacacgat aaagaagtca accttatctg cattggtaaa ctgatagtca
    36061 taatagttat tccaaaggat gtagtcctca tttgtagcat tttcactata atgctccgca
    36121 tgaacaacta ccgctaaatg tgctgtctgt gcttcctcaa acacaacctg accaatgcct
    36181 gtctccctat cgagaatgac caaatcagac ttattcaaat tcaaagattt cataaaggca
    36241 cgcacaaaag cttgctttcc atagaaaatc ttatccttga aatgataaac ttcttccttc
    36301 ccttgattca tcaaaatatc atagactgga gtcccgtctt cattgtaaaa agttcgttgg
    36361 tataagactg caacattgtc cttgggagca aaatactcgc tacaataacg cgtataagaa
    36421 aagtaatcct tccgaatcag gtttccctta aaaacatact cggcatgttg aaccaagtcc
    36481 ttgttctcat caactaaata acaggttaca aacttatctt ggtcaaaaaa gaagacacgt
    36541 aaaaccttgc catttttttc tctgtgactt tcttcaccac caaagtaagc caagacatca
    36601 tccactgtca cgctagtagg cgcaattttg atatctgtga aatgattata aagccagata
    36661 acctgattat catcaaaacc aatattggct gttaagtgct gaatattatc ggctaaaatc
    36721 atatctgtaa agataaactt agaggacaga tttaatttcc gaaaaacacc agcacgatag
    36781 gcttgagcgt attcaacacc gctactagcc caaccaattc ctaaatttat attgtaaatt
    36841 gtcatatttt ctcctcttat ggaaaatgag tcacgatttc accttttgga gtgacctcaa
    36901 ccagccccat caggagatta cgcaccatat ccgcacggat ttgttctttc atagcttcaa
    36961 agcccgcgta ggcttcttga tagtactcta cgattggatt tttctgacta gcagattgac
    37021 caccgatagc catggatagc tgttgtagat agtctacctg ctctatccag ttgtcatcaa
    37081 tggctttaag cagtgaaagt cgtaaaaact gttcatataa gtcatgttga ttaagtaatt
    37141 ctttcttttc agaaagttct ttatcaatca cctgcttcat aaagctacga actgcagttt
    37201 tgtcagttac atctatataa tctggaacct ctttaacatg aaaactaata ttggtcacaa
    37261 taaagtgaaa cagtaattca cgactagcat agtgatcagc cgctacctct tctgtatatc
    37321 tctcaatgat atccacaaca acatcctcta agtcacgaga accatctatt agacgattac
    37381 gctccttgta aatcatatcc cgttgaatat tcatactttc agcatattct agagtctgac
    37441 gacgtgccga acggccagca ctatcactag catgttgagc cttttcgact agtttccggt
    37501 atttacgacc tttcaatact tccggttgag tcatatcttg aacctgatag tctttgtact
    37561 ttttatgcac ccaagatgga ccaaatttct tgataacatc atcctctaag gatacaaaaa
    37621 atttactcat accaggatct ccctgacgac cagaacggcc acgaatttgt aggtcgatcc
    37681 gctgactttc catccgctca gtcccaataa caatcaagcc cccaagctct gcgactcctt
    37741 taccaagctt gatatccgta ccacgtcctg ccatagaggt agccactgtc acagccccca
    37801 tctgacctga ctcggagata atctgagcct cacgcgccgc attattagca tttaaaacat
    37861 tatgggcaat tccctctctg agcaagagtg acgaataaag ttgagacatt tcaactgagc
    37921 ctacaaaaac gagtaaagga tttcccttag catggtattc cttgatgtac tccaaggatg
    37981 catacacttt ttcaggtaaa gtgatatata gattatctgg atagtcaatc cgttgtctcg
    38041 gacgattggt tggaatgcgt actacagaca tattgtaagt ttcaataaac tctttttccg
    38101 cgaccttacc tgtccctgtc ataccagata ttttattaaa catcttaaaa agactctgat
    38161 aggtgatcga ggccatagcc cgcgtctcag gagataattt gacatgttcc ttggcttcaa
    38221 tagcctgatg gagacctcct tgaagtttag tcatttccat tagacgccct gttcccttat
    38281 caaccagtac catctcattt ccacgaatga tatagtcctt atctttagta aagagcttat
    38341 gagctcgaat cgcataaacc aaatgacgag caaaagacgc atgctcttcc ttgtataaat
    38401 tatcaatccc taggaaattc tcagcagact tggccccctt agtagtgagc caaacctcct
    38461 ctttctcctc tttaaagata taatcctctc cttcgaccaa ggttgttaca agtgtatcaa
    38521 tgatcgcata gtaattagac tgaacacgag gagaacccgc aataatcaga ggagtttgtg
    38581 cactatcaag caagatatca tcaatttcat caataatcac atagttaaac ggtcgtaaaa
    38641 acttaccttc ttcattcgag gctaggttat catttagata atcaaaacct aaattactat
    38701 tggttgtgta gatgatatcc gaagcataga taagcttctt ttcttcagct ttcatctcct
    38761 tctttggatc ttccgtaaat ggtacaccaa tggtcaatcc tagaaaacga taaacttgac
    38821 ccatttcctc ggcatcacgc tttgataaat actcattagg agtcacaacc atcactcctt
    38881 ctcctgaaaa agcgttcaaa tagacaggca tggtagctgt caaggtctta ccttcccccg
    38941 tattcatctc agcaacattt ccatagtgca tgacaatagc tcccatgact tgaacatcat
    39001 aaggaaacat ccctaaaatc cgcttatctg cttcacgcac cacagcaaaa gcttcaacca
    39061 aaatatcgtc tagactttct ccctcggaaa gacgctgacg aaactctact gttttagcta
    39121 ctaattcttg atccgaaaga gaggacatct tgcctttcag ggcattaatc tgctttaaaa
    39181 tctttttcac tttcctaagc tggaaatctt gacctaaacg tctaaacact tctgatctcc
    39241 ttgatagaaa aatgataaaa agttaaggac tcaaatccag cacttaaaag agaaacttga
    39301 taagtataac tatcttctgg ataagtaaat atgaaatctt tcgctttttc tacctttttt
    39361 tctaaaatat caccatagcg gtcaaaaaaa gttatcttgg tataaactcc attttcaggc
    39421 tgacaatcaa aaaccagtga tagttgatac tcctgctttc tttttaataa gggaagctga
    39481 ggttgggttc gtgcagcctg gtaattgaca ctggaaaacc aagtctttag aatctcacca
    39541 gatggcaata gaggattata cagacgaaca cttttgtcag ggtaataaga tacagttgat
    39601 ccatacatat aagtcccacc aacttctccc caatgaatag actgtctctg tgtaataatc
    39661 atcttttctc ccttccaaaa ccagtctcta agaccattct ataaaagtgc aaaaaccaag
    39721 aaatattggt atctgtatca tcattgtgtc gtccagcagt tcctttagat aaaatcttcg
    39781 cacctgtatt acacagataa gacactaatt gttcataggc ttgtggatcc atttcttcat
    39841 ctttcatata ggataaacca aaggttgtct ttgaaaaatt tgcttgttta aaaattttcc
    39901 aaaaacgctg atccaactcc gtcatatctt gagaacttgt cccacctgta tgatgaatta
    39961 aacaatcaaa agctaagtta gagacacccg gtgcgtccaa acgtccacga ctagctatag
    40021 ttcctaaatt agccaagggc ttaccaacta tgatggcatg gggttcaaaa gaagcaccat
    40081 aatagagagc cggaaacgtt cccatagaaa gacctgataa aatcaaatcc ttatgatcaa
    40141 agcctaaata atcaagatag tgagtgatcg tatcctttac ttttccctct agctcatccg
    40201 ttccgagata aaaagctccc ccctctaaac gtggatcaga aaataaaatg aagggacatc
    40261 caagagtttt catcataaag taaccctcga atccttctgc aggtcgataa cctgcaaaat
    40321 agacagtcaa aggcggttta aaatcacctg gatggaagaa atagtttatt tcatcacgct
    40381 tggaatcatg taggatattt ccaccaagta caaacttacc aaattgtttt cgactccatc
    40441 gttggtgaag attacctagt ttgatagttc cacgacctct tgcttcaaca gatatcgaga
    40501 gaaagtagtc tccgtctcga ctatccataa aaagtgcttg ctccaagtct ttttcagata
    40561 ctattacttc ttcaaagaaa tcatcaacag acccactcca cattttacgg attactaaac
    40621 gaaagtcaca attgccttcc ttctcatatt caagccacaa ctcaataggg agtgtttttt
    40681 gcaccataat attataagcc caataggcaa gctgggcaaa atcttgccca aactcacctt
    40741 ccaaactgac ataatccaat ccttgataag aaatagctcc tgtaaaattt ggattgactt
    40801 gtatcgtcgg aggaaagagt ttatcaccat acccaccgcc aaaaagagat ttacttaaat
    40861 cattgattag ttgttgggga cttaaaaaat caattgcttg tgcacatcgt ttttttagaa
    40921 aatccacaac ctctctatca tttatttgta gattctgg
//
LOCUS       DAVMEF010000002        34339 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMEF010000002 DAVMEF010000000
VERSION     DAVMEF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.002"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            138..950
                     /locus_tag="VYQ30_000043"
     CDS             138..950
                     /locus_tag="VYQ30_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1965221.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            1289..2308
                     /gene="adhP"
                     /locus_tag="VYQ30_000044"
     CDS             1289..2308
                     /gene="adhP"
                     /locus_tag="VYQ30_000044"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1965222.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            2541..3530
                     /gene="manX"
                     /locus_tag="VYQ30_000045"
     CDS             2541..3530
                     /gene="manX"
                     /locus_tag="VYQ30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1965223.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            3558..4361
                     /locus_tag="VYQ30_000046"
     CDS             3558..4361
                     /locus_tag="VYQ30_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1965224.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            4385..5296
                     /locus_tag="VYQ30_000047"
     CDS             4385..5296
                     /locus_tag="VYQ30_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1965225.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(5352..6686)
                     /gene="pepC"
                     /locus_tag="VYQ30_000048"
     CDS             complement(5352..6686)
                     /gene="pepC"
                     /locus_tag="VYQ30_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1965226.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(6903..7625)
                     /locus_tag="VYQ30_000049"
     CDS             complement(6903..7625)
                     /locus_tag="VYQ30_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1965227.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(7893..8123)
                     /locus_tag="VYQ30_000050"
     CDS             complement(7893..8123)
                     /locus_tag="VYQ30_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1965228.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(8133..9374)
                     /locus_tag="VYQ30_000051"
     CDS             complement(8133..9374)
                     /locus_tag="VYQ30_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1965229.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYVLGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NATSDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVIFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(9396..9548)
                     /locus_tag="VYQ30_000052"
     CDS             complement(9396..9548)
                     /locus_tag="VYQ30_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965230.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLLGAIALAGIF
                     SALKEK"
     gene            complement(9569..9779)
                     /locus_tag="VYQ30_000053"
                     /pseudo
     CDS             complement(9569..9779)
                     /locus_tag="VYQ30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            complement(9772..10035)
                     /locus_tag="VYQ30_000054"
     CDS             complement(9772..10035)
                     /locus_tag="VYQ30_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1965231.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(10137..14528)
                     /locus_tag="VYQ30_000055"
     CDS             complement(10137..14528)
                     /locus_tag="VYQ30_000055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1965232.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDVQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(14632..16713)
                     /gene="fusA"
                     /locus_tag="VYQ30_000056"
     CDS             complement(14632..16713)
                     /gene="fusA"
                     /locus_tag="VYQ30_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1965233.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPVEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(17138..17608)
                     /gene="rpsG"
                     /locus_tag="VYQ30_000057"
     CDS             complement(17138..17608)
                     /gene="rpsG"
                     /locus_tag="VYQ30_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1965234.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(17628..18041)
                     /gene="rpsL"
                     /locus_tag="VYQ30_000058"
     CDS             complement(17628..18041)
                     /gene="rpsL"
                     /locus_tag="VYQ30_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1965235.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            18458..22255
                     /gene="spuA"
                     /locus_tag="VYQ30_000059"
     CDS             18458..22255
                     /gene="spuA"
                     /locus_tag="VYQ30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1965236.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHIGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHRRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKAIDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASLPAHQDPAPEARPDSTKPDAKVADVENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(22355..23404)
                     /locus_tag="VYQ30_000060"
     CDS             complement(22355..23404)
                     /locus_tag="VYQ30_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1965237.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVDLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            23461..23538
                     /locus_tag="VYQ30_000061"
                     /pseudo
     CDS             23461..23538
                     /locus_tag="VYQ30_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(23675..25483)
                     /gene="glmS"
                     /locus_tag="VYQ30_000062"
     CDS             complement(23675..25483)
                     /gene="glmS"
                     /locus_tag="VYQ30_000062"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1965238.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(25675..27054)
                     /gene="bglA"
                     /locus_tag="VYQ30_000063"
     CDS             complement(25675..27054)
                     /gene="bglA"
                     /locus_tag="VYQ30_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1965239.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGYWF
                     KELSDNNGF"
     gene            complement(27153..29006)
                     /locus_tag="VYQ30_000064"
     CDS             complement(27153..29006)
                     /locus_tag="VYQ30_000064"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1965240.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(29019..30278)
                     /gene="rseP"
                     /locus_tag="VYQ30_000065"
     CDS             complement(29019..30278)
                     /gene="rseP"
                     /locus_tag="VYQ30_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1965241.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETSQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(30300..31103)
                     /locus_tag="VYQ30_000066"
     CDS             complement(30300..31103)
                     /locus_tag="VYQ30_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1965242.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(31112..31870)
                     /locus_tag="VYQ30_000067"
     CDS             complement(31112..31870)
                     /locus_tag="VYQ30_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1965243.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(32101..32673)
                     /locus_tag="VYQ30_000068"
     CDS             complement(32101..32673)
                     /locus_tag="VYQ30_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1965244.1"
                     /translation="MNTVKNKQEILEAFRESPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(32654..33625)
                     /gene="ruvB"
                     /locus_tag="VYQ30_000069"
     CDS             complement(32654..33625)
                     /gene="ruvB"
                     /locus_tag="VYQ30_000069"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1965245.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            33786..33878
                     /locus_tag="VYQ30_000070"
     CDS             33786..33878
                     /locus_tag="VYQ30_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1965246.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         9958 a         7956 c         6644 g         9781 t
ORIGIN      
        1 attcactttt tcaatgtctt tgtttgattt gtttaagaaa ttaatccaat ttcatttttt
       61 gtttggaatc tgcttttctg cctgctttct gttataatag aagacatctt atctgaaaac
      121 acactagaaa ggtccatatg acgaaaaaaa ttattgcagt tgacctagat ggaaccctgc
      181 tcaactcaga cagtcaaatt tctgacttta ccaaaaaaac catcaaaaaa gttgctgaaa
      241 aaggccatca ggttattatt acgacaggtc gcccttaccg tatgtcaaaa gatttttacc
      301 gtgaactggg cttagacact cctatgatta acttcaacgg atcccttact catttaccag
      361 accaagtttg ggattttgaa aagtgtttga ctgtagacaa aaaatatctg ctagatatgg
      421 ttcaacgttc agaggacatt caagccgatt ttatcgctgg agaatatcgt aaaaaattct
      481 acattacaaa tcccaatgaa gaaattgcca atcccaaact atttggtgta gaagctttcc
      541 agcctgaaga tcaattccag cctgaattgg tgaccaagga ccctaactgt atcctcttgc
      601 agaccagagc cagtgacaaa tatgccttgg caaaagaaat gaacgccttc taccagcatc
      661 aactttctat caatacctgg ggaggtccgc tcaatatcct tgaatgtacc ccaaaaggtg
      721 tcaacaaggc ctttgctttg gactacttgc tcaagataat gaatcgtgac aaaaaagatt
      781 tgattgcctt tggagatgaa cacaatgata ccgaaatgct cgcttttgct gggaagggtt
      841 atgccatgaa aaatgccaat ccagagctac tcccttatgc agatgagcaa atttccctta
      901 ccaacgacca agatggggtt gccaaaaccc tacaagactt attcttataa cctatactga
      961 tactcaatga gaatcaaaga gcaaactagg aagctagccg taggttgctc aaagcactgc
     1021 tttgaggttg tagatgaagc tgacgtggtt tgaagagatt ttcgaagagt atgaccagct
     1081 tgcaagctgg tctttgtgct cttttcacag tctcatcaac cagttaatcg attgttctac
     1141 tttttgtctc caaaaccttg gaaaaggctt tcttttgtga tatacttcac atataaatac
     1201 aagagagctc gtcacactcg actctgttga caaaaaatca atccagtccc gttgtccctg
     1261 ttctcggtca atatcaagga ggatagctat gaaagctgtt gttgtaaatc cagaaagcac
     1321 tggtgttgct attgaagaaa aagtactccg tccacttgaa actggggaag cacttgtaga
     1381 agttgaatac tgtggcgttt gccacaccga cctccacgtt gctcatggtg actttggtca
     1441 agtcccagga cgtgttcttg ggcacgaagg tatcggtatc gttaaagaga ttgctccaga
     1501 tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct tggttctttg aaggatgtgg
     1561 cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc cgtacagtga aaaatgctgg
     1621 ctactcagta gacggtggta tggctgaaca atgtatcgta actgctgact atgctgtcaa
     1681 agttcctgac ggacttgatc cagcccaagc ttcttctatc acatgtgctg gagtaacaac
     1741 ctataaagct atcaaagaag caaaagttga accaggccaa tgggctgttc tttacggtgc
     1801 tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa gtattcaatg ctcatgttat
     1861 cgcagtcgat atcaacaatg acaaacttgc ccttgcaaaa gaagtaggcg ctgacattgt
     1921 gattaacggc ctcgaagttg aagatgtagc tggactcatt aaagaaaaaa ctgatggagg
     1981 agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc ttcaaccagg ctgttgactc
     2041 cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct tctgaaatga tggaactcag
     2101 catcgttaaa acagtcctcg atggaatcca agtcatcggt tctcttgtcg gaactcgtaa
     2161 agacttagaa gaagccttcc aatttggtgc agaaggtctg gtagtcccag ttgttcaaaa
     2221 acgtccagta gaagatgctg ttgccatttt cgacgaaatg gaaaaaggcc aaatccaagg
     2281 acgtatggta ctcgacttca cccactaatc taatagaaac ctattttaaa agttggaagc
     2341 ttcttccaac ttttttatat taaattttaa ataagaattc aagattactc ctcaaaaact
     2401 caacatatca acatacaaaa ataaaaattt tttaattttt tatgataaat agttgctttt
     2461 tttatgaaaa cggtttatac ttaaacagtc ttaaagtttt cttaaatgaa aactaaaaca
     2521 aaaaaggagg aatgacaata atgagtatcg gaatcattat tgcgagccac ggcgaatttg
     2581 ctgcgggtat tcatcagtca ggatctatga tctttggtga acaagaaaag gttcaagttg
     2641 tgacctttat gccaaatgaa ggtcctgatg atctatacgc taagtttaat aacgctgttg
     2701 ctgcatttga cgcagaagat gaggttctag ttttggctga cctttggagt ggttctccat
     2761 ttaaccaagc tagtcgcgtg atgggagaaa atcctgagcg taagtttgcc atcatcacag
     2821 gacttaactt accgatgttg attcaagcct acacagagcg cctcatggac gctgctgcag
     2881 gtgtagaaaa agtcgctgct aatatcatta aagaagccaa agatggcatc aaagctcttc
     2941 cagaagagct aaatccagtt gaagaagttg caagcgctgc agctgctcca gttgcccaaa
     3001 ctgctatccc agaaggaact gttatcggag acggtaaatt gaaaatcaat cttgcccgtc
     3061 ttgacacacg tctacttcac ggtcaggttg caactgcttg gactccagat tcaaaagcaa
     3121 atcgtatcat cgttgcttca gataatgtgg ctaaagacga ccttcgtaaa gaattgatta
     3181 aacaagcagc tccaggtaat gtcaaggcta acgtggttcc aattcaaaaa ctgattgaga
     3241 tttcaaaaga cccacgtttt ggagaaacac atgcccttat cttgtttgaa acacctcaag
     3301 atgcccttcg tgccatcgaa ggcggcgtgc caatcaagac tcttaacgtt ggttctatgg
     3361 ctcactcaac aggtaaaaca ttggtcaata ccgttttgtc tatggacaaa gaagatgttg
     3421 ctacatttga aaaaatgcgt gacttgggtg ttgaatttga tgtccgtaaa gtaccaaatg
     3481 attctaaaaa agatttgttt gacttgatta acaaagccaa tgtcaaataa gccattattt
     3541 atgaaaggat tttaaacatg tctattattt ctatggtttt agtagtcgtt gtagccttct
     3601 ttgcaggtct tgaaggcatc ctcgaccagt tccaatttca ccaaccactt gtagcctgta
     3661 cccttattgg gcttgtaaca ggtcacttgg aagcagggat tatcctcggt ggatcgcttc
     3721 aaatgattgc ccttggttgg tcaaatatcg gtgctgctat cgctcctgat gctgcacttg
     3781 cttctgtcgc tgctgccatt atcatggttc ttggtggtga ctttaccaag actggtatcg
     3841 gtgttgccca agcggttgct atccctcttg ccgtagctgg tctattctta actatgattg
     3901 ttcgtacaat ttcagttgga ttggttcaca ctgctgatgc tgccgctaaa aaaggtgact
     3961 tcggcgctgt ggagcgtgct catttcattg cgctactttt ccaaggactt cgtatcgcgc
     4021 ttcctgcagc tcttctcctt atggtaccaa ctgaaactgt acaaagtatc cttagtgcca
     4081 tgccagactg gctcaaagat ggtatggcta tcggtggtgg tatggtcgtt gccgttggtt
     4141 acgccatggt tatcaacatg atggcaactc gtgaagtatg gccattcttc gctcttggtt
     4201 tcgttctcgc tgctgtgtca gatattactc taatcggatt cggtgctatc ggtgttgcta
     4261 tcgctcttat ctaccttcac ctttctaaaa ctggtggaaa tggtggcgga ggagccgcaa
     4321 cttctaacga cccaatcggc gatatcctag aagactacta agataagaaa ggactgaaaa
     4381 catcatgact gaaaaacttc aattaactaa atcagatcgt aaaaaagttt ggtggcgttc
     4441 aaccttctta caagggtctt ggaactttga acggatgcaa aacttgggct gggcttatac
     4501 actcattcca gctatcaaaa aactctatac taaaaaagaa gatcaaatcg ctgctcttga
     4561 gcgtcacctt gagttcttca acactcatcc atacgtagct gctccagtca tgggggttac
     4621 tcttgcgctt gaagaagaac gtgctaacgg tgtggaaatc gatgacgctg ctatccaagg
     4681 ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt gacccagtat tctggtttac
     4741 agtacgccca atccttggat ctctcggtgc ttcacttgcc cttactggca atatcttggg
     4801 gccactcctc ttctttgttg catggaactt gattcgtatg tcattcttgt ggtatgttca
     4861 agagattgga tacaaggctg gatcagaaat cactaaagat atgtctggtg gtatccttca
     4921 agatatcact aaaggagctt ctatccttgg gatgttcatt cttgctgtcc ttgttcaacg
     4981 ttgggtaaat attaaatttg ctttcgatgt ttctaaagtt caactagatg aaaaggctta
     5041 tatccattgg gataaattgc cagaagggtc taaaggtatc caagaagcat tcgcacaagt
     5101 aggacaagga ttgtctcaaa ctcctgaaaa agttactact ttccaacaaa acttggatat
     5161 gttgattcct ggattatcag gactactcct tactttactt tgcatgtact tacttaagaa
     5221 aaaagtatct ccaatcacta ttatccttgc cctcttcgca gtgggtattg tggcacatgt
     5281 tcttcacatc atgtaatcaa gcaactaaaa aggaaccagg ttctaaaatc tgattccttt
     5341 tttctatgct tttattcagc caaggctccc attggatccc atggtgcaag tacgattggt
     5401 tctgctccat aggcagcttg ttcttctgct gtcagcaatt ccttacgaac aacgatttgg
     5461 tatgtgtatt cgtccatcca agcgtctgag gcaacaaagt aaccatctgt accgaccttg
     5521 tctccccatg agttttcaac cttccacttg gttgatttac cattttcgtc caagtcaaca
     5581 cctgtcaaga ccatggcgtg ggtcatcaag ctttcgctat agtccaaacg tccagccttg
     5641 tcttgagtaa gtttaatgtc catgcttgat tcaaagtcat aaacatctgt cgcaaggatt
     5701 ccagccttac ggttgctgag ctggccgaca tcagaaccaa accaaacagt ctcacctgct
     5761 tgcatttggg caatcgccaa ttctttcaag cgctccattg gaacgttgat gtagcgaact
     5821 gcacggctac caacaacatt ccccaacatc tcaactgtgt aagattttcc gtaaggttta
     5881 tcagcagttg gagcattgat aacagaaacg tagtcttcta aaggaagatt gacatatttc
     5941 ttgtaaaact cttgtggtgt gattcctttt tcacttttgt agttgttatc tttatcgcga
     6001 taagcaaagt caaacttgcg tggtggaagt cccaatgaca tagcaagaaa gttaaagatt
     6061 tcttgcaaga ggtcttcttt cttagcttga acagtcgctt gatctgcacc agaaacaagc
     6121 aaatcacgca agatttgagc atcttgacga agcaatttat taaggatcgc atttagctca
     6181 cgactgctgc tagatgaaac agactcagga taaactgact taggcacgac accgtatttt
     6241 tcaaagaggg aaacgaccat atcccattga ccgccatctt gttgaggtgt ttggagtaag
     6301 aagctaacct tgcggctagt caattcttgg tctgaagtcg caatgacttg ctccaagaac
     6361 cagtttgatt tctcatactt atcccagaag aaagtgtggg cttgtgacaa ctcaaagttc
     6421 tccaatttgt attgcgagat gagtttgtgg cggaaggtgt tgagagccgc aaacatccag
     6481 caacgaccag acgctttctg gttagtgacc ttgtccttgg ttaaatccaa tgagaaaaca
     6541 ggtgtgttgt ctacatggct ttggcgacgt tccagagctg caaaaattcc gttgtggctg
     6601 gcagcatttt caatcgcttg gtattttaca tttgcttcat agttggcaaa tagtttatca
     6661 gtaaatgatt cttgaatcgc gttcatagat tcctcctttt attctacagt ctattataac
     6721 tcttttcaca aaggaagcaa ctaaaaaaca taaaaggcga ggacattgtc cctcaccttt
     6781 ttcgaattta tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtactc
     6841 aagtacagcc tgcggctagt ttcctagttt gctctttgat tttcattgag tattaaacta
     6901 aattaaataa tattagcgcg gagaatttct aattcttcct tggtcaagcg acgccattcc
     6961 cctcgttcta ggttctcatc taatactaaa gttcccatag tcaatcgttg caagtccacc
     7021 acttccttgc cacagtagcc caccatacgc ttgatctgat gaaacttccc ttctgcaatg
     7081 gtcacacgga tttggctttg attcttttct gtatctatgg atacaagctc cagtatagcg
     7141 ggttgacagg taaagtcttt gagaggaata ccctcagcaa atgtctccac atcttcttgg
     7201 gtcatgattc ccttgacttg tgccagataa gtcttgtcca catgacgctt gggcgaaaga
     7261 agaacatgag ccagctgacc atcattggtc aagagcaaaa gaccatgcgt gtcaatatcc
     7321 aagcgtccta ctgggaaaac ttccttactc cgcgccaagt catccaacaa gtccagaacg
     7381 gttctgtgct tgggatcctc agtcgctgag ataactcctt tgggcttgtt catcatgtag
     7441 tagacaaact cttcatactc caacacttgc ccatcaaagc gaatctcatc tattttttca
     7501 tcaatctgca atttagctga tttttctttt ttaccattta cagtcacgcg cccagccttg
     7561 agcaagtttt tgacctcagt ccgacttccc accgcacagg caactaaaaa tttatctaat
     7621 ctcatagaac tattatatca tatcaaaagg aggctagtac aatgaccaac ctccttttcg
     7681 tttcatactc ttcaaaaatc tcttcaaacc gcgtcaacgt cgccttgccg tatatatgtt
     7741 actgacttcg tcagttctat ctgcaacctc aaaacagtgt ttttagctga cttcgtcagt
     7801 tctatctgca acctcaaagc agtgctttga gcaacctgcg gctagtttcc tagtttgctc
     7861 tttgattttc attgagtatc agatttagga aattaacttc ctcgtctcca aaaaatagct
     7921 aaaacaatca tggctcctaa aatcgctggg ataatagctg ttcctgataa aactggcccc
     7981 caagttccaa agagcaagtg acctagaaag gctccgatcc aaccgagaaa catttttcca
     8041 aaacatccca ttcgctctcc acgattggtc atagcacctg ctaaaaatcc cactaggaga
     8101 ccaacgaaca tacttcctaa catattatct ccttaatttg cccaattccc attacggaaa
     8161 agaggtaccc gcgttccatc ctcacgaata ccatcgatat ccatttggtt agaaccaatc
     8221 ataaagtcta cgtgaacatc tgaacggtta agccctgcag cttcaagctc ctcttcgctc
     8281 atctccgctc catcaacaac gctagtcgca taggctgcac cgatagccaa gtggtttgac
     8341 gcattttcat cgaaaagggt gttaaagaag gtaatgcctg actgagaaat tggacttgga
     8401 tctggtacca aggcacattc acccaaggca cgcgcacccg cattttcaaa gacaaggtct
     8461 ttcataacct gatcaccctt ctcagcagtg atatctacga tttgtccatc cttaaagatc
     8521 accttaatgc cttcaatgat atttccgttg tagctaagcg gttttgtaga agtgacataa
     8581 ccatctgcac gacggaagtc aggcgctgtg aagacctctt ctgtcggcat atttggcaag
     8641 aatccttcgc cctgtgcatt gacagcacca gctgattccc aaacgtggtt ctttggcaaa
     8701 ccaagtgtta aatctgttcc tggcgctgtg tagtgaaggg ctgaaaattg ctccttatta
     8761 agcatatcgg ccttgctctt gagaatggct gcatgctctt cccaggcctt aacaggatct
     8821 gcttcgtaga cacggcaagt tttgaagatt tggtcccaaa ggaaatcgac tgcttcttcg
     8881 tcgctcgtag catttggaaa gaccttctta gcccattcaa gtccagcagc ggctgctaca
     8941 gtccagctaa ccttgttaga ttgagttgcg attcgcattg gcttcatggc aagtcccata
     9001 gctttagcag aagctgaaag cttgtcagcg tccactccgt tcaaggcacc tggatcagat
     9061 gaacgaactc caagacggct agccttgttc tccaagagat agttcatctc agcaatcttg
     9121 tattctggca cattgtccaa acgctccatc ggggcatgga ggaatttctc acggttaatc
     9181 acatcatctg tccactgaac gatgacctca tgcgcaccca agacataagc ttctttcacg
     9241 attagatgtg ccaattcacg ttgctccaca tcaatagaga gagccaaagt gtgaccaggt
     9301 tgcacgttaa ttccgttcgc aaccaacaat ttcgcatatt tttctagatt ttctttaaaa
     9361 tttggtaaaa ccatcctgtt ttctcttttc tatttttatt tttctttcaa agcagaaaag
     9421 attcctgcta aagcaatagc acctaacaag agtgctgtcg atagaagaat tgtacgatca
     9481 gcaagttcta attgatattc aaataccttc ttagccggct tatcttccgc aaatattctt
     9541 agtttcatga gaaccccctt aaaatagatt aaaataaatc actgtgactg cctgtcctca
     9601 gcaaatttaa aatcaattcc gacttgtcta ctttataaac caaaagccaa tctggcagga
     9661 tgttcttttt cttgaaccag aaaattcaaa acttcttcta ataattctgc cttcaaacca
     9721 cgcttcatag ccaacttaaa atctttttta aactgtttat gataacgaat cttaagcacg
     9781 taagtcctcc atcaaatctg agactgattc aaaagactgg ctcatattac gattttggtc
     9841 taaatccgtt aacgcttgga gcaacttccg attttcgtct agtctaacat caaaaggtag
     9901 tccctgatat tgaattgcct gacgaaggaa aatattaata gctgttgtca tatccattcc
     9961 cagattacta aacacctgtt gggcctgctc cttaacctca ctatccagac ggatgcttat
    10021 actcatcttt gacatactct caccctcttt ccatagacta ttttaacaaa aaagaaagct
    10081 aatgtaaatc tattggatat acgttagcct cttttaatag attattaagc aattttttaa
    10141 aacaactcat caaacaaact caactggtta tcctctggca tatttccaag aatacccatc
    10201 tcatccatct tttcaaccaa ggttgatgag agtccaccac gcttgcgtag ttctgtttta
    10261 gagaggaatt ctccctcttc acgcgcccgc accagttgct tggcaacgtt ctctcccaga
    10321 ccatccattg ctacaaatgg tgggataagg gtatccccgt cgatgaggaa ctctgtcgcc
    10381 tgactacagt agagatctaa tttaccaaac ttgaaacctc gttcccacat ctcattgaca
    10441 atctcaagag ttgtatagag atcgatttcc acattagagg cttcattgtt cttccgtttt
    10501 tcagagattt cttccattct gcgcttgatg acctccaaac ccgcacccat ggtcttgata
    10561 tcaaaagcct tagcacgaat ggagaagtaa gcacagtagt aataaatagg atggtgaacc
    10621 ttgaagtaag ctacacgcaa ggccatcata acgtaggctg ccgcatgggc cttagggaac
    10681 atgtacttaa ttttcccaca ggattcgata taccactctg gcaccttatt agccttcatg
    10741 gcttcgatat agtcatttct ctcctcttct gaaatcttta gccacaaacc cttacgtacc
    10801 cgttccataa tagtaaaggc catcttaggt tccagacccg catgcatgag gtaaaccatg
    10861 atgtcgtccc gacaaccgat aacagtcgat aggtccgcta ttccttgctt aatcagatcc
    10921 tgagcattcc ccaaccaaac gtcagtaccg tgggacagac cagacagctg aagcaattcc
    10981 gcaaaggttg tcggatgggt ttcgtctacc attccacgta cgaaatttgt tccaaactct
    11041 ggaatcccca acatacccgt aggcgttcca atttgttcag gtgttacccc tagcacatca
    11101 gtcccagaaa agagtgccat cacgccttcg tcatccatag gaatttcatt agggtcaata
    11161 ccagacaaat cctgaagttt tcgaatcatg gtcggatcat catgtcccag tacatcgagt
    11221 ttgaggacgt tctcatcgat atcgtggaag ttaaagtgag tggtctgcca ttcagccgtg
    11281 acatcatctg ctggatactg gacaggcgta aaatcgtaga catccatata gttcggaata
    11341 acaacgattc cccccgggtg ttggcctgtt gtccgcttga cacccgccgc tccttgagcg
    11401 aggcgttcta cttctgcatc acgataaaac ttgccataat ctcgctcgta acccttgaca
    11461 aatccatagg cagtcttggc agctaccgta ccaaccgttc ccgcacggaa ggcatattct
    11521 tcaccaaaga tatcacgcac atccaagtgg gcgctaggct gatcttctcc cgagaagttc
    11581 aagtcaatat caggaacctt atccccatca aaaccaagga aggtctcaaa cggaatatcc
    11641 tgtccgtttt tactgagttt gtgaccacag tttggacagt ccttatgggg catatcaaat
    11701 cctgaaccgt acgaaccatc tgtgataaac tcactgtact gacactgacc acagacatag
    11761 tgaggagaga gaggattgac ctccgtaatc ccaatcatgg tcgcaacgaa actagatccg
    11821 acagacccac gagaaccaac caaataaccc cgttcattag aacgttgcac cagcatctgc
    11881 gatgccagat aaatcacagc aaatccattc cccagtatgg atgttaattc tttttcaatc
    11941 cgcaaatcaa caatatctgg cagcggattt ccataaatct caaaagcttt cttataggtc
    12001 aactcagcaa ctgtttcttc agccttgtcg atgaaaggcg tatacaagtc acccttaacg
    12061 acttcaacgg gttcaaatat ttctgccaag gcattggtgt tttcaataac cagtttacga
    12121 gccagttcct ctcccaaaaa ggcaaattca tccaacatct cattagtcgt tcgaaaatga
    12181 gcctttggaa gtggtgctgg ttgggcatgt tcaccatgac cgatagttcg gttaatcatc
    12241 gcaccctgtc ccaaactacg gacgataatt tcacgataaa tctcttcttc cggttcgata
    12301 tagtgaacat ttcccgtagc caaaacaggc ttaccaaggc ggtctccaac ctctatcaaa
    12361 ctcttgataa tggtctggag ttcctccata tccttgacct gctctttagc aatcaagggc
    12421 gcatagatag ccggtggcat gacctcgata aagtcataat acttggccac ctcaaccgcc
    12481 gcatccacac cttgagaaac gaccacgtca aaaacttcac cctctgaaca ggctgaacct
    12541 aaaatcaagc cctctcgatg ggcatctaga accgttctcg gaatccgtgg cactccttca
    12601 aaatacttgg tattagacaa ggaaaccagc ttaaagatat tttttagacc tacctgattc
    12661 ttgacataga tggtcgcatg cttgatccga gcttttttgt aagaatctgg actgattaga
    12721 tcaatgttga gtctagctaa atcggtcaca ccatgttttt ctgctacctc tttgataaag
    12781 atgaaaagca gacgaccagt cgcttccgca tcgtagttgg ccatgtggtg atgttccaag
    12841 gccacaccaa aacgcttggt caaaggcccc aaaccatggc gtttatactc aggatagagg
    12901 tttctagcaa actccagcgt atcaataact ggctgactaa tctttggaag atcatgacgc
    12961 tcataattag cattcataaa gccaacgtca aaggtagcat tgtgggcaac taggaccgta
    13021 tccttgcaaa attcttggaa ttcttgcaaa acttgttcta gtggtttggc atttttgaca
    13081 tgatcatctg taattccagt taactctgta gtaaaagctg acaagggatg cccaggattg
    13141 ataaattcat caaattcagc aataacattc cccttgtaca tcttagaggc cgcaacctga
    13201 atcaagtcat tatagatagc tgaaagtccc gtcgtttcca cgtcaaagac cacgtaggtt
    13261 gcttctgata agtccatctc cacttcgtta tagacgatag ggacacggtc ctccacgata
    13321 ttggcttcca tcccatagat cagctggatt cccgctttct tagccgcctt atagccatgt
    13381 ggaaaggact ggacattccc atggtctgtg atagcaaccg ccttgtgtcc ccacttagca
    13441 gctgttgcaa caatctcttc gacctctggc aaagcatcca tagtcgacat gttagtatga
    13501 gcatgaaact caacccgacg ctcaccttct ggcatcaaat ccttccgctc atagtgaaca
    13561 acttcctgca catcctgtac gttcatagtc aaatcgcgtg tgaagttatt catctccaca
    13621 ttccctcgaa ctcggagcca agaattcttc ttgatgaggt caaacttctg ggcctcttcc
    13681 tcgtttttaa cccacttttg catagaaaaa cttgaagtgt agtccgtcat tttaaagttg
    13741 attaaaacac gacctgttct agtcactttt tgctccacat caaaaacaac cccttcaaat
    13801 accagacgat tttcctctgt cgtcacttcg atcataggag taatctccgc cttatccagt
    13861 ttgggtttag ctgcagcttt tttcgcttga aaatcaaaga ctggtttctc ttccactgga
    13921 ggaggtgcca tctgctccag ttgttccata gcacggagcg cttcctcatt ggcagcttga
    13981 acaatctgct cattttcagc atgaaaggcc tcttcctgct cttgggtcag gacatcattc
    14041 ttctcgactt gacagttaaa agtttgaaaa ccaaactttt caagttgttt ggctaaatta
    14101 ggaagatgat tcttcttaaa atgttcctta tcaatcgctt cagatccttc aataaatagc
    14161 tgattaccct cagcacgaac ttgcaaattt tgataaaggg acttaaaacc ttgactagca
    14221 catggacctt cagagaaagc ctccctatag taggactgca agagctgatt tgaaaattct
    14281 tgagaccgag ccttaatttc aaaaacagct ttattgcctg tcttagaaaa ttcttcgctc
    14341 aaacctttct ttaattctaa aaagatttca atcggtaaaa tattagaaaa tacgaaatga
    14401 aactcccata ccttactaat tttatgaacc acaactcgct caatattggc ctgtgctaaa
    14461 gcaggagcct gtctcatttc agcaggcatc cccaattgat tcatcaaaat ttcaaaacta
    14521 tttgacattc attttcctca cattattctt ctactatttt accatattta gaggtatttt
    14581 ctaaagacaa aaggaagcca ctaagtgact tccttctaga gtgaggacgg attagtcttc
    14641 acctttattt ttcttaataa tttcttcttg tactgactta ggtacatctt cgtagtggtc
    14701 aaataccatc atgaatgtac cacgtccttg agatgcagaa cgaagaactg ttgcgtaacc
    14761 gaacatttca gcaagtggaa cgtaagcacg aacgatttgg ctgttaccgt gtgcttccat
    14821 accatctaca cgtccacgac gagcagttac gtgacccata acatcaccaa ggttttcttc
    14881 tggaacagtg attgttacaa gcatcattgg ttcaaggata gctggttgtg ctgatttagc
    14941 agcttcttta agggaaagtg aagccgcaat cttgaaggca gtttcagatg agtcgacatc
    15001 gtgatatgaa ccatcataaa gcttagcttt aacgtcaacc attgggtaac ctgcaagaac
    15061 accgttagcc atagattcta ccaaaccttt ttcaaccgct gggataaatt cacgaggaac
    15121 cacaccaccg acgattgcgt tttcgaattc gaatccttta ccttcttcgt ttggagtaaa
    15181 ttcaatccac acatcaccga attgaccttt accaccagac tgacgtttga agaatccacg
    15241 tgcttgagta gaagcgcgga atgtttcacg gtaagatact tgaggcgcac ctacgttcgc
    15301 ttcaactttg aactcacgac gcatacgatc aacaaggacg tcaaggtgaa gttcacccat
    15361 acctgagata actgtttcac cagtttcaac gtttgtttca acgcggaatg ttggatcttc
    15421 ttcagccaat ttttgaaggg cgatacccat cttgtcttgg tcagctttag attttggctc
    15481 aaccatcaat tggataactg gttctggaac gttgattgac tcaaggatga ttttagattt
    15541 ttcatctgtc aatgagtcac cagttgtagt atctttcaaa ccaacggcag cagcgatatc
    15601 acctgagtaa acagtgtcga tttcttgacg gctgttagcg tgcatttgaa ggatacgtcc
    15661 gatacgttca cgtttacctt tagaagtatt caatacgtat gaacctgatt gaagaacacc
    15721 tgagtaaaca cggaagaatg tcaaacgacc tacgaatggg tcagtcatga tcttgaaggc
    15781 aagagctgca aatggctctt cgtcagatgc tggacgagtt tcttcagcgt ctgtatctgg
    15841 gttaatacct ttgattgctg ggatgtcaag tggacttgga aggtagtcga taaccgcatc
    15901 aagcatcaat tgaacacctt tgtttttgaa ggctgaacca cacaatactg ggaagaattc
    15961 aacgttgata gtcgctttac ggataccagc tttcaattct tcgttagtga tttcttcacc
    16021 ttcgaggtat ttcatcatca attcttcgtc agtttcagca actgcttcaa tcaatttttc
    16081 acggtattct tgagcttggt caaggtattc aactgggatg tcttcttcaa ggatatccgt
    16141 accaaggtcg ttagtataga tttcagcttt catcttgatc aagtcaatga taccacggaa
    16201 gtcatcttca gaaccgattg gcaattggat tgggtgtgca tttgcttgaa gacgatcgtg
    16261 aagtgtgctt acagagtaaa ggaagtcagc accgattttg tccattttgt tggcaaatac
    16321 gatacgtgga actccgtact cagttgcttg acgccaaact gtttcagttt gaggttcaac
    16381 acctgattgt gagtcaagaa cggtaaccgc accatccaat acacgaagag aacgttgtac
    16441 ttcgattgtg aagtccacgt gtcctggtgt gtcgatgatg tttacgcggt ggttgttcca
    16501 ttgagctgtt gtcgcagcag atgtgatcgt gataccacgt tcttgctctt gctccatcca
    16561 gtccatttgt gacgcacctt cgtgagtttc accgattttg tggattttac cagtgtagta
    16621 aagaatacgc tcagtagttg ttgttttacc ggcatcgacg tgagccatga taccgatatt
    16681 acgagttttt tcaagtgaaa attcgcgtgc catgaggttt gtttctccta tttatttttg
    16741 atttctattc tattataaca cgattttaat aaaaacggat aggcaggacc tacccgttct
    16801 caatgttttc atgctattgt tggtttcaac ttacgagatg gtaagctgag ttatagctaa
    16861 tactaatcga tttagctaat ttgaacccgg gctaaagtta gttagccgat gtgagctgga
    16921 acgggatgct gcgtgaaaaa gataaaactc cttgtattcg ttgaatactg cgtcagtttc
    16981 ctattttcac cttgcatcct taccgtgcct agcatcatga tttagttgag ctcaagctaa
    17041 aagtctggag aaaaagatga atctcattgg ttgcaaccgc aacctgcttc gattccctat
    17101 tttctctggg actttcttaa cgctttcgca tcctatctta ccaacggaag tgtgcgaatg
    17161 cacggttagc ttcagccata cggtgagtat cttcacgttt cttaactgct gcaccagtgt
    17221 tgttagcagc atccaagatt tcttttgcaa gacggtcttg cattgtgtgt tcaccacgaa
    17281 gacgagcgat tgttaccaac caacgaagtc caagtgttgt acgacgttct ggacgaactt
    17341 caactgggac ttggtagtta gaaccaccaa cacgacgtgc acgtacttca agtacaggca
    17401 tgatgttttc catagctgtt tcaaatactt caagtgcatc gttgccagta gcttctttga
    17461 tttgctcaaa agcaccgtaa acgattgaag cagcagtacc acgtttacca tcaagcataa
    17521 cgcggttgat aagacgagta actagttgtg aattgtatag cggatctggc aatacgtcac
    17581 gttttggagc tctattttta cgactcattt ctctttatcc cctttcctta tgcttttgga
    17641 cgtttagtac cgtatttaga acggccttgt ttacgatcgt taacacctgc agtatcaagt
    17701 gcaccacgga cgatatggta acgtacccct ggaaggtctt ttacacgtcc accgcgaaga
    17761 agcaccacgc tgtgctcttg caagttgtgt ccgatacctg ggatgtaggc agtaacttcg
    17821 ataaggttgc tcaaacgtac acgagcgaat ttacgaaggg ctgagttagg ttttttaggt
    17881 gtcattgttc caacacgagt tgcaacacca cgtttttgtg gtgaagaaac gtttgtttga
    17941 acttttttat gactattgta accaacgttc aaagctggtg atttagattt ttctactttt
    18001 gatttacgcg gtttgcgaac caattggtta attgtaggca tctacattct cctgtgtttt
    18061 tttatttttg gtgatgatac acttggtgac agatatcatc tgtgtgtact tttgcaacat
    18121 ttgtcagcac gtccctgtac actcttgaga gaccaaaagt aaaaagtacc gtctattatt
    18181 gtaacaaaat tttcccttgc ttgtcaagat atttttcttt tttattggta atgttagaga
    18241 tcaatttctg aataaaaata gctgggaaca catcatcttc ctaataatac ggcttggctt
    18301 atgtgacatt tttatctccg tcatttatac tgatatataa aatagtacag aaaacttctc
    18361 aaaagtaaga atttttttca taaatcaaag aaaacgcttg catattgttt tactacatgc
    18421 tatactgata tagaaattta ttgattggag aatcattatg agaaaaacac catctcacac
    18481 tgagaaaaaa atggtctaca gcatccgttc cctcaaaaat ggaactggtt ctgtccttat
    18541 cggcgcaagc cttgtcttgc ttgccatggc tacaccaact atctcatccg acgaaagtac
    18601 accaaccact aacgaaccca acaacagaaa tacaaccacc cttgcccaac ctcttactga
    18661 tacagcagct ggctctggta agaacgaaag tgatatttct tcacctagaa atgcaaacgc
    18721 ttccctagag aaaacagaag aaaaacctgc aacagagcca actacttcta ctagtccagt
    18781 aacaactgaa actaaggcag aagagcccat cgaagataac tacttccgta tccatgtcaa
    18841 aaaacttcct gaagaaaaca aggatgctca aggactatgg acttgggacg atgttgaaaa
    18901 accatctgaa aactggccaa acggagcttt gtccttcaag gatgccaaga aagatgacta
    18961 cggctattac ctagatgtca aattaaaggg agaacaagcc aagaaaatta gcttcctcat
    19021 caacaataca gctggaaaaa atctaaccgg cgataaatct gtagaaaaac tagttccaaa
    19081 aatgaacgag gcttggttag accaagatta caaggttttc tcttacgagc cacagcctgc
    19141 aggaactgtt cgcgtcaact actaccgcac agatggcaac tatgacaaga aatctctctg
    19201 gtactgggga gatgtgaaaa atccaagtag cgctcaatgg cctgacggaa cagactttac
    19261 ggctacaggc aaatatggcc gctatatcga cattcctctt aatgaagccg caagagaatt
    19321 tggattttta ttactagatg agagcaaaca aggagacgac gtgaaaatcc gtaaagaaaa
    19381 ttataagttc acagatttga aaaatcatag ccaaattttc ctaaaagacg atgatgaatc
    19441 gatttacaca aatccatact atgtccatga tatccgtatg acaggagccc aacacatagg
    19501 cacttctagc attgaaagta gcttttcaac acttgtcggt gctaaaaaag aagatatcct
    19561 caaacactcc aacatcacta atcacctagg aaacaaggta actattaccg atgttgcaat
    19621 cgatgaagct ggtaagaaag tgacctacag cggagatttc tctgacacaa aacatcctta
    19681 tactgttagc tacaattccg accaattcac taccaaaaca agctggcgcc tgaaagatga
    19741 gacatacagc tatgatggca aactgggagc tgacctaaaa gaagaaggaa aacaagttga
    19801 tttgaccctt tggtcaccaa gtgctgataa ggtttctgtt gttgtctacg acaagaatga
    19861 ccctgacaaa gtagttggaa ctgtcgctct tgaaaaaggg gaaagaggaa cttggaaaca
    19921 aactctagac agcacaaaca aactcggaat cacagatttc actggatact attatcaata
    19981 ccaaatcgag cgtcaaggta aaactgttct tgcactcgat ccttacgcta aatctcttgc
    20041 tgcttggaat agcgacgatg ccaagattga cgatgcccat aaagtggcta aagccgcctt
    20101 tgtagatcca gctaaactcg gacctcaaga cttgacttat ggtaagattc acaatttcaa
    20161 gactcgtgaa gacgccgtta tctacgaagc tcatgtgcgt gatttcactt cagatcctgc
    20221 cattgcaaaa gacttgacca aaccatttgg tacttttgaa gcctttatcg aaaaactaga
    20281 ctacctcaaa gacttgggtg taacccacat ccagctcctt ccagtcttgt cttactactt
    20341 tgtcaatgaa ttgaaaaacc atgaacgctt gtctgactat gcttcaagca acagcaacta
    20401 caactgggga tatgaccctc aaaactactt ctcattgact ggtatgtact caagcgatcc
    20461 taagaatcca gaaaaacgaa tcgcagaatt taaaaacctc atcaacgaaa tccacagacg
    20521 tggtatggga gctatcctag atgtcgttta taaccacaca gccaaagtcg atatctttga
    20581 agacctagag ccaaactact accactttat ggatgccgat ggcacacctc gaactagctt
    20641 tggtggtgga cgcttgggga caacccacca tatgaccaaa cggctcctag ttgactctat
    20701 caaatatcta gttgatacct acaaagtgga tggattccgt ttcgatatga tgggagacca
    20761 tgacgccgct tctatcgaag aagcttacaa ggctgcacgc gccctcaatc caaatctaat
    20821 catgttaggc gaaggttgga gaacctatgc cggtgatgaa aacatgccta ctaaagctgc
    20881 tgaccaagat tggatgaaac ataccgatac tgtcgctgtc ttttcagatg acatccgtaa
    20941 caacctcaaa tctggttatc caaacgaagg tcaacctgcc tttatcacag gtggcaagcg
    21001 tgatgtcaac accatcttta aaaatctcat tgctcagcca actaactttg aagctgacag
    21061 ccctggagat gtcatccaat acatcgcagc ccatgataac ttgaccctct ttgacatcat
    21121 tgcccagtct atcaaaaaag acccaagcaa agctgagaac tatgctgaaa tccaccgtcg
    21181 tttacgactt ggaaatctca tggtcttgac agctcaagga actccattta tccactccgg
    21241 tcaggaatat ggacgtacta aacaattccg tgacccagcc tacaagactc cagtagcaga
    21301 ggataaggtt ccaaacaaat ctcacttgtt gcgtgataag gacggcaacc catttgacta
    21361 tccttacttc atccatgatt cttacgattc tagcgacgca gtcaacaagt ttgactggac
    21421 taaggctata gatggtaaag cttatcctga aaatgtcaag agccgtgact atatgaaagg
    21481 tttgattgcc cttcgtcaat ctacagatgc cttccgactt aagagtcttc aagatatcaa
    21541 agaccgtgtc cacctcatca ctgtcccagg ccaaaatggt gtggaaaaag aggatgtagt
    21601 gattggctac caaatcactg ctccaaacgg cgatatctac gcagtctttg tcaatgcgga
    21661 tgaaaaagct cgcgaattta atttgggaac tgcctttgca catctaagaa atgcggaagt
    21721 tttggcagat gaaaaccaag caggatcagt cggaattgcc aacccgaaag gacttgaatg
    21781 gactgaaaaa ggcttgaaat tgaatgccct tacagctact gttcttcgag tctctcaaaa
    21841 tggaactagc catgagtcaa ctgcagaaga gaaaccagac tcaacccctt ccaagcctga
    21901 acatcaaaat gaagcttctc tccctgcaca tcaagaccca gctccagaag ctagacctga
    21961 ttctactaaa ccagatgcca aagtagctga tgtggaaaat aaacctagcc aagctacagc
    22021 tgattcacaa gctgaacaac cagcacaaga agcacaagca tcatctgtaa aagaagcggt
    22081 tcgaaacgaa tcggtagaaa actctagcaa ggaaaatata cctgcaaccc cagatagaca
    22141 agctgaactt ccaaatacag gaatcaaaaa cgaaaacaaa ctcctatttg caggaatcag
    22201 cctccttgcg ctccttggtc tcggtttctt actaaaaaat aaaaaagaga actaaactag
    22261 ccctcctata gaaaaatccc ccaagcatta tagctcgggg gattaatttt cgtacaatat
    22321 ttgttgtcct aataaacttg attaggattt tttattaagc ctctttcata gcaaaataag
    22381 ctcgtacttt gggtgcaact tgtgttccga agagttcaat agctctcaga acctggtcat
    22441 gaggcataga accaagcggt agatgaagca tgaagcggtc caatcctaaa tcctctatca
    22501 tgcgaatcaa tttttcggcc acctgatctg gattgccaac aaacatggcg ccatttgacc
    22561 ctacctgctc caaatattgc tcataacgca attcctgcca gtgcggacgg tctttggaaa
    22621 tagcatccac cacttgctta gtcggatgga aataatcttt caccgcctgc tcaccatctt
    22681 ccgcaatcca cccccaagaa tgggctccca ctttcaagtc tttgtcagca tggcccgctt
    22741 cgcttccaat ctcacgataa gcctgaatca actttttaaa ataacgtgga ttaccaccaa
    22801 taatagcata tacaatcggt agaccagcct gagcaatctt cactgttgat tcgacatgac
    22861 cacctgtagc tatccacaag ggcaatttgt cctgaactgg acgaggataa acttctttac
    22921 cagcaatcgt ttgagtcaat cgaccttgcc agtctaactt ggtcttttca ttgactaact
    22981 gaagcaagtc taatttctca tcaaaaagag agtcgtagtc tttcaagtca taaccaaaca
    23041 gagggaaaga ttccgtgaaa gagccccttc cagccataat ctccgctcgt ccatttgaca
    23101 aagcatcgat agtggcatac tgttggaaca aacgaatcgg gtccatgctt gacagaatgc
    23161 tgactgcact ggtcaaacgg attttcttgg tattgactgc cccagcggcc agaacaatct
    23221 ctggagctga tactgcaaaa tccgcccgat ggtgctcacc aatcccatat acatccaaat
    23281 caaccttgtc agccagctca atctctgcca ccaactggcg aatgcgttca gcatgactgt
    23341 aagtttgtcc agtcccttca agctccgtta tttccccaaa tgttgaaatt cccaattcta
    23401 ccattgtgat tctccttgtc tatctctgtc cttcaatttg aaaaattatt ctaatactca
    23461 atgaaaatca aagagcaaac taggaagcta gctgcaggct gctcaaaaca gtgttttgag
    23521 gttgtggata gaactaacga agtcagtaac catacctacg gcaaggcgac gttgacgtgg
    23581 tttgaagaga ttttcgaaga gtataacacg aatcttgagt acaagcaacc aatttgctca
    23641 caagaaaaag cctagataac tagacttttt tagcttattc taccgttact gacttggcaa
    23701 ggttacgtgg tttgtccaca tcgaggccac gatggagggt tgcaaagtaa gcgactaatt
    23761 gcgttggtac gaccattgaa attggtgaga ggtatggatg tacggtcgta aggacgatat
    23821 cgtcggtatc tttggctaca ttctcttctg cgatagtgag gactttggca ccacgggctg
    23881 cgacctcttg gatatttcca cgagtatggt tggcaagaac tggatctgac aagagagcca
    23941 aaacaggcgt tccttcttca atcaaggcaa tggttccgtg cttgagttct cctgcagcaa
    24001 agccttcaca ctggatataa gaaatctctt tgagtttgag acttgcttcc atggctacgt
    24061 agtaatcttg accacgtccg atgtaaaagg cgttacgagt tgtttcaaga agttcacgaa
    24121 ccttgacttc aatggtttct ttctctgaaa gagttgattc gatagactga gctacgattg
    24181 acaattcatg aaccagatca aagacttgcg ctttagcatt accatttgct tctccgactg
    24241 cttttgcaag gaaggcaagg gctgcgattt gcgctgtata ggctttagtt gatgccacag
    24301 caatttcagg acctgcatga aggagcatgg tatagttggc ttcacgtgag agggttgaac
    24361 ctggaacatt tgtcactgtt aagcttggaa ttcccatttc attagccttg accaaaactt
    24421 gacgactatc cgctgtttca ccagattggc tgataaagat gaagagtggt ttcttgctga
    24481 gaagtggcat accgtagccc cactcagatg agattccaag ttcaactggt gtatctgtca
    24541 attcttccaa cattttctta gaagcaaatc ctgcatggta agatgttcca gctgcaagga
    24601 tgtagatgcg gtctgcgtct tgaacagcct taatgatatc tgggtctacg acaacttgac
    24661 cagcctcatc tgtgtaggct tggatgagtt tccgcataac agttggttgc tcgtcaattt
    24721 ccttgagcat gtagtaaggg taagttccct taccgatatc tgacaagtca agttcagcag
    24781 tgtagctagc acgctcacga cgatttccat catagtcttg aacttccaca ctatcagcct
    24841 tgacgattac caactcttgg tcatggattt ccatgtattg gttagtttca cgaatcatag
    24901 ccatggcgtc tgagcagacc atgttatagc cttctccaag accaatcaaa agtggtgatt
    24961 tatttttagc tacgtagatg acttcaggat cttgtgagtc aaccaaggca aaggcataag
    25021 aaccacggat gatgtgaagg gcttttttga aggcttcaag aactgagagc ccttcttctt
    25081 ccgcaaattt tccaatcaaa tgaacggcta tttcagtatc tgtctgcccc ttgaagtggt
    25141 gacctgcaag gtattcttcc ttgatttcaa ggtagttctc aatcacccca ttatgcacca
    25201 agacaaaacg ttctgtctca gagcggtgtg ggtgagcatt gtcctcagtt ggttttccgt
    25261 gagtagccca acgagtatgt ccgataccag ttgttccctc aacaccagct gtcttggcag
    25321 acaattctgc aatacgacca acagccttca ccaaatggtt atcagcacca tctaggacaa
    25381 aaattcccgc agaatcatag ccacggtatt caagcttttc aagcccttga atcaaaatat
    25441 cagttgcatt tgtgtttcca acaacaccaa caattccaca catagtatat acgacacagg
    25501 caagctgtgt tttctcctta aaattggtat agtctaattc atcttttata gaatcagcaa
    25561 aaacagtata tacttgtttc tttcacttgt caagagtaaa aattggtata gttcaaatta
    25621 agctcctgta agcaaaaaac tctgaccgat tgggataatc agtcagagtc ctttttaaaa
    25681 tccattatta tcgcttaatt ctttgaacca gtagcctgat ttcttcagac gacgttcttg
    25741 cgtttccaag tctaattcga ccaaaccata gcgattttta tagctgttga gccatgacca
    25801 gcagtcaata aaggtccaaa tcaagtagcc cttacagttg gcaccatctt caatggcacg
    25861 gtgaagttca cgaagatgac cttttacaaa gtcaatacgg taatcatctt gaatcattcc
    25921 atcttgacgg aatttttctt ccccttcaac acccatacca ttctcagtca acatccactc
    25981 aatattgcca taattttcct tgatattttg ggcgatgtca taaatccctt gctcataaat
    26041 ctcccaacca cggtgagaat tgattttacg tccaggcatc acataaggct cgtaaaaatg
    26101 ttctggtaag agtggactct ctggatgctt agcaaatcga ggagccataa cacgcaaagg
    26161 ttgatagtag ttcacaccaa ggaagtccac cgtattatca cgaatgagtt ccaactcttc
    26221 ctctgtagca tcaggtaaaa gaccgtgttc atgcaagatt tctaccaact cctgtggata
    26281 agtccccaag acagatggat ctaagaaaga ttgggcctga aaaagggccg caatacgagc
    26341 tgccttgaca tcagcaggat gctggctacg tggataagcc ggtgtcaagt tgaggacaat
    26401 cccaatcttg gaatcaggca aaagttcatg gcaagcctta acagcccggc tgctggccaa
    26461 ttgtgtatga taggctacct taacagctgc ctctgcatcc accttatgtg gataatgggc
    26521 atcataaaaa taaccaaatt ctacaggaac gatgggctcg ttaaaggtaa tccattgatc
    26581 cactaaatct ccataagtct caaaacaaaa acgagcatag tcttcatagg ctgagactgt
    26641 cgccttattt tcccaaccat caccatcctc ttgaagggca aaaggtaaat caaaatgata
    26701 gagattgact aacagacgaa ttcctttagc cttaatagcc tcaaagacct tacgataaaa
    26761 atccacacct tgagggttga cttttccaca gccttgtgga aaaatccgtg accactgaat
    26821 agaagtccga aaggctgtgt gaccagtctc taacaaaagc tcaatatccc gctcccaatt
    26881 ttcataaaaa gtcgatgtct tatctgaacc aatcccatta tagtaacgat ttggctccac
    26941 ttggaaccag taatcccaga gattgtctcc cttaccgtca ccagctacac gtccttctgt
    27001 ttgcggtcca gaagtagagg atccccagac aaaatccttt ggaaatctta gcatacattt
    27061 acctctttat ctactcattt ctcccattat acagaaaaaa caaggtaaaa actagttaca
    27121 ttttttcctt gtttttcttc tgattatagt ttttatttct tgcttaggat ttcaagcgtt
    27181 tcaagcacgt tatctgcatg aacctcaatg gtgtcaccag ttgccttgat cttaacttct
    27241 acaatgccat cggccgcttt tttaccaaca gtgatacgga ttggaagacc aatcaagtca
    27301 ctatcgctaa atttaacacc gacacgttcg ttacggtcat ctgtcaagac ttcataacca
    27361 gctcccatca agcttgcttc aagtttttct gtcaaggctt gcgcttcttc atccttgaca
    27421 ttgacagtaa tcaaatgcac atcaaatggt gccaattctt tagggaaatt gattccccaa
    27481 gcgtaacggt attcaccttt tggcgttttg ttaacaaaga ggcgagcgtg ttgctccatc
    27541 actgctgaaa gaagacggct gacaccgata ccgtaacatc ccatgatgat tggcacagca
    27601 cgaccatttt catccaagac atctgctccc atgcttgctg aatagcgagt tccgagtttg
    27661 aaaatatgac cgatctcaat accacgcgca aagttaagga caccttgtcc atctggggaa
    27721 atttcaccct cacgaacttc acggatatcc acatattctg cagtaaaatc acggcctggg
    27781 ttcacaccag tcaagtggta gccatcttcg ttagcaccga caactgcatt gcgaacatct
    27841 tgtaccttac gatctgcaat aattttaata ttctctggca aaccaactgg tccaagtgaa
    27901 ccaaatcctg cttgaacaac attcgccact tcttcttcgc tagcaacgtc aaagaaatct
    27961 gctcctaagt ggtttttcaa cttgacttcg ttgagttggt catttccaac tagaagggct
    28021 gcaacaagct caccatctgc aatgtagaag agggttttaa tcgtttgttc ttctggaaca
    28081 ttgaggaagg ctgcaacttc atcaattgat ttaacatctg gcgttgcaac acgagtaact
    28141 tcttcttcag cgacaacacg gttgcttggt ttgtactcgt ttgttgccat ttctaagtta
    28201 gctgcatagc tagactcact tgagtaagca atggtatctt caccagagac tatccatttg
    28261 agcaattctg ccttgatttc ttcttgcact tctgcaggaa tttcgtcaaa tgaggcaact
    28321 gacttgtcca agacaaccca gcggtcaagg tctgtacgag cagatgtaat ggccataaat
    28381 tcttggctat ccttaccacc catggctcca ccgtcaccaa taatagcctt gaagtctaaa
    28441 ccactacgag tgaaaatacg ctcataggct gctttgtact catcataaac actatccaaa
    28501 ctatcatagt tagcgtggaa actataagca tccttcatga taaactcacg tgtacgaaga
    28561 agtccattac gtgggcgttt ttcatcacga tacttgggct gaatttgata aaggttgagt
    28621 ggcaattgct tgtaagattt aacagaatca cggacaatag ctgtaaaggt ttcttcgtga
    28681 gttggaccta agataaagtc tgatttttca cggtttttta gtttgtaaag gtcttcacca
    28741 taggtttcgt aacgacctga ttcacgccac aattctgcac taagaagggc aggagccaac
    28801 atctcaacag caccaatctt ttcgaattct tggcgcatga tgtttttagc tttttcaatc
    28861 acacggttgg caagtggtag ataagaataa acacctgctg aaacttggcg aacataacca
    28921 gcacgcaaca taagagcatg gctgataact tgagcatcgc ttggcatttc gcgaagcgtt
    28981 gggataggca ttttactttg tttcataata ttcctcgatt atctaaaaaa gagtcgcata
    29041 atgtcattcc aagtcacagc aatcatcaag acaaccatga tgaccactcc ggccaaggtg
    29101 acataggttt caatttcttg tttcaatggt ttgcggcgga tggcttctag gatattgagc
    29161 acaatcttac caccatccaa ggctggaatc ggaataagat taaaaatccc aatattgatg
    29221 gaaatcattg ccaagaagta caagatattc tcaattccat ttttagcagc atcactactt
    29281 gccttaaaga tagcaacagg tccacccaac ttgttcaaat ccggttggaa aatcagattt
    29341 ttcagagctg agagaattcg gagagctgag tcagcagcag ttgtaaaacc acctacaaac
    29401 atggatagaa aatctgactt aacccccggt tgaacaccta gaaggtaacg accttgacta
    29461 tcttcgggtg taacagtgac ttgtttgtca ctcccctttt cagaaatagt cacatccaaa
    29521 gtcggtgccg tcttatcttt ggtttctgtt tccacagctt ggatcaagct ttcccagttg
    29581 ctaacctcat gtgagccaat cttagtaatt tgtgacgttt ctggtactcc taccttggcc
    29641 aaggcacctt ggggcatgat atggaactga ttggtatcaa catctctgac accaccctgc
    29701 ataaagatta aaacccaaaa aacaacgaca cctaagataa aattgttcat agggcctgca
    29761 aaattggtaa tcagtttgcc ccagatagtc gcattttgat attgaacatc taaaggtgca
    29821 atccgaacct cagtaccatc tgcttccaca accgttgcat cgtgatccac tgcaaatgtt
    29881 ttttcttctt ccagaaccaa tcctttgata aagagcttgt cttcaaaatc aaactgggtc
    29941 acctgcatag ggagggctgt ttgatccaat tttttacctg agagattgat gcgtttaacc
    30001 ttaccatcat cagcaagtgt caaactaaca ggcgttcctg tcttgatttc agttgtatca
    30061 tcaccccaac cggccatgcg gacatagcca cccagaggca agattcgaat ggtataggcc
    30121 gttccatcct tgccaatgtg agcaaagatt ttaggtccca taccgatggc aaattcacgt
    30181 actaaaatcc ctgatttctt ggcaaagtag aagtgcccga actcgtgcac cactacaata
    30241 atcccaaaaa ccagaataaa ggttaaaatt ccaagcatag tgtttcctcc gtcttttgat
    30301 taaaagagtc caaataagtg catgattgga aatacaagca acatactatc gaaacgatcc
    30361 aaaacaccac catgtccagg gataaatttc ccagaatcct taacaccaaa atgacgtttg
    30421 atcgaacttt ctagtaaatc accaaattgt ccagcaatgc taaagaaaat agcaaagact
    30481 gacatcttgt aaattccata tggaagagca actgtactgt caactatcat aaagataatg
    30541 gttactaaaa ttgctcctaa aataccaccc aaggcaccct caagggtttt attaggcgat
    30601 acccttggtg ctaactttcg tttcccatag ttcatcccaa caagataggc accactgtct
    30661 gtcgcccaga cgatacacaa ggctaagaga gccttgtcca aacctgcaac acgagcatct
    30721 agtaaagcat taaatccaaa gcccacgtag aagctcatag caagagggaa aaccgcatcc
    30781 tcaatcgtat aagacttgct aaaaacggtc gttcctaaca tgattgaaat caaaacacta
    30841 taggcaacca cattcccatc aactggcaaa aaagtcaggt aattctccaa gggaatggtc
    30901 aatgcaaagg ttgcaaagag ggtcaagagg ccctccatcg tcatggtctc tagacctctc
    30961 atcttcaaaa gttcatgcat ggctagcatg gctatgattc cgattgctat ctgaagcaag
    31021 aggcccccaa tcattaaaat tggtaggaaa atagccaggg caatccctgc aaacaaggtt
    31081 cttttctgta aatcctgggt catatttcct cctaaactcc tccaaatcgg cgatgacgac
    31141 gattataggc aagaatagct tcctgcaagg ccgcttcgtc aaaatcaggc cataaggtgt
    31201 ccgtaaaata aagctcacta taggctccct gccatggaag gaaattgctc aaacgtaatt
    31261 ctccactagt acggataatc aagtctgggt ctcgtaagtc cttaggcaaa tgctgagtaa
    31321 agagatagtt accaatcaat tcctctgtga tgtcacctgg gttgattttg gcatctaaaa
    31381 catcctggga aatcaactta agcgcctgtg taatctcagc acgtccacca tagttaagag
    31441 caaaattaag aatcaatcct gtgttgttct tagtcaattc ctcagccttg gttaaagctt
    31501 caaaggtttg cttaggcagg cggtctgtct ccccaatcat ttgaatctta acattattcg
    31561 catgtagttc cgggacataa ttatcataaa actctactgg caagttcatg ataaacttga
    31621 cttcctgatc tggacgggtc cagttttccg tagaaaaagc atagaccgta ataaccttga
    31681 cgcccagttt gttggctgcc ttggtcacgg tttgcaatgc ttccatgccc gccttatgtc
    31741 caaaaactcg cggttgcata cgttttttag cccaacggcc attgccatcc atgatgatgc
    31801 cgatatgagc aggaacctgt gtcggaacct ctacttccac agccttatct ttcttaaaaa
    31861 atccaaacat gatcttattc ctattcaaaa atctatcgtt tcattatacc atatttcccc
    31921 attttcttct atcactaagc tatttattct caggcaccaa gcccattttt caaaaaaata
    31981 agccgcctga ttgggcgact ttatttttat agggagatta ttatgaaaaa gttttaggag
    32041 tttaagttaa ggtcttctta acttatgaac ttagtgtaca ctccctagct taaagtttcc
    32101 ttaagtattt ttaaaaatca aatttttcca tttctcctgc caatttttct tggataaacg
    32161 tgtttgatag agttccattc ggtcttcatt ttctaagaaa tgaggagttg gacgaacttg
    32221 aaaattcaaa atatcctcca aaccataagg tacatagagt tcaaaatctg attcttcatt
    32281 caagcgcagt ccaactgccg tacaccgttc tggatactta ctcatagcat cacgagaact
    32341 ggtataggaa gcagtgtgag gactgtgctg atgcatatag acctgatttt tcaattccca
    32401 ctggtactga ggaaaatcct ctctcagctt tttctccagt aataaggttt cctcataaga
    32461 aaaatctgga tcaaagaaaa tcacatctat atctgtttca tgatcaaaag gggatttgtc
    32521 tgacaaaaga ttccagatga aatttctgac agaacctgct gccaaccacg agtctttcaa
    32581 accaaggtct cggatgatcg tcagaatggc catcatatct ggactttctc taaaagcctc
    32641 taagatttct tgcttatttt tcactgtatt cataacctaa gtgctcatat gccttagcag
    32701 tcgccacccg tccagaccgt gtccgcatga taaaaccttt ttgaatcaag taaggctcat
    32761 acatatcttc aactgtctca cgctcttcgg cgatattcac agaaagagtt cctagaccaa
    32821 caggtcctcc actgtacatc tcaatcatgg tacgaaggat tttttgatcc acatagtcca
    32881 aaccttcatg gtcaacatcc agcatagtca aagccttatc ggtaataata tcatcaatta
    32941 ccccattccc cattatctgg gcaaaatcgc gcacgcgctt gaggagacga ttggcaatac
    33001 gaggggtccc acgactacgt agggccaact cagatgctgc ctcatgagtg atttccatct
    33061 caaaaatatc tgccgtccgc tcgacaattt ctgtcaagtc agcatgggca taatactcca
    33121 tatggcctgt aatcccaaaa cgtgcccgta gtggatttga gagcatacca gcccgagtcg
    33181 tcgcaccaat caaggtaaaa ggtggtaact ccaaatgaac actacgacta ccctcaccag
    33241 ccccaatcat aatatctatg tagaagtcct ccatagcact ataaagcacc tcttccactg
    33301 acattggcaa acgatggatc tcgtcaataa agaggacatc tccaggctct aactcattca
    33361 aaatcgctac caaatcaccc gctttttcga taacaggacc agacgtttgc ttgagattga
    33421 ctcccagttc attggcaatg acaaaagcca tggttgtttt cccaagccct ggagggccaa
    33481 ataagagcac atgatccagc gcttcatccc gcattttagc ggcttcgata aagatctgaa
    33541 gctgatcctt aaccttatcc tgaccaatat attcacgtaa atactgagga cggagcgtgc
    33601 gttctactaa ctcctcatca cccatcatct cattatctaa aattctactc atggctctat
    33661 tatatcaaaa aaacaagcca caaacaaaaa agccacctga ttgggtgact cctaagttta
    33721 gcacttatgt ggtataatat tatacgacac tgctacaccg cctacgaaag gaggtgagat
    33781 agcccatgat ggaattagta ctcaaaacta tcatcggacc aattgtggtc ggtgtcgttc
    33841 ttcgtatagt cgataaatgg ctaaacaagg acaaatagtg tcaaaaaaga cctcaagctt
    33901 atttggtcgt gagcttgggg tcttttctag cctatgatat agaactagta ctcaattcct
    33961 ttttattatc ccatagttca cgaattttgt caaaggttta cattttcttc aaccgctgta
    34021 cgacaagacg gttaagatta agagaacgtt agggattcta tcaatttcat agaaattttg
    34081 atttcgtaaa cgaagagaca atcttacatg tcacttctca tttaatacgc cactactaga
    34141 caagcaaaat cattattaca gtagttccag ttcttcaatt aacagtcact tacaatcaaa
    34201 ttgagtttga actagctgaa gcgaccacag acctatttct tagtcatatt cgctaaaaaa
    34261 gtccccgcca attccccgac caaaatccga aaaataccga aaaatatcga aaaattattt
    34321 ttagaatagt ccccaaaag
//
LOCUS       DAVMEF010000003        31744 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMEF010000003 DAVMEF010000000
VERSION     DAVMEF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.003"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            349..1512
                     /locus_tag="VYQ30_000071"
     CDS             349..1512
                     /locus_tag="VYQ30_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1965247.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1509..2186
                     /locus_tag="VYQ30_000072"
     CDS             1509..2186
                     /locus_tag="VYQ30_000072"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1965248.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLNFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2176..3030
                     /locus_tag="VYQ30_000073"
     CDS             2176..3030
                     /locus_tag="VYQ30_000073"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1965249.1"
                     /translation="MKLDSYTRLAAVVANPIKHSISPFIHNRAFEATATNAAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQDQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3049..4116
                     /locus_tag="VYQ30_000074"
     CDS             3049..4116
                     /locus_tag="VYQ30_000074"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1965250.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4126..5292
                     /gene="aroC"
                     /locus_tag="VYQ30_000075"
     CDS             4126..5292
                     /gene="aroC"
                     /locus_tag="VYQ30_000075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1965251.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5302..6405
                     /locus_tag="VYQ30_000076"
     CDS             5302..6405
                     /locus_tag="VYQ30_000076"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1965252.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6416..6754
                     /locus_tag="VYQ30_000077"
     CDS             6416..6754
                     /locus_tag="VYQ30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1965253.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6846..8129
                     /gene="aroA"
                     /locus_tag="VYQ30_000078"
     CDS             6846..8129
                     /gene="aroA"
                     /locus_tag="VYQ30_000078"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1965254.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPISSAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8122..8598
                     /locus_tag="VYQ30_000079"
     CDS             8122..8598
                     /locus_tag="VYQ30_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1965255.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8595..9443
                     /gene="pheA"
                     /locus_tag="VYQ30_000080"
     CDS             8595..9443
                     /gene="pheA"
                     /locus_tag="VYQ30_000080"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1965256.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMAANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9440..10714
                     /gene="psr"
                     /locus_tag="VYQ30_000081"
     CDS             9440..10714
                     /gene="psr"
                     /locus_tag="VYQ30_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1965257.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10993..11838
                     /locus_tag="VYQ30_000082"
     CDS             10993..11838
                     /locus_tag="VYQ30_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1965258.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11828..12931
                     /locus_tag="VYQ30_000083"
     CDS             11828..12931
                     /locus_tag="VYQ30_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1965259.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12933..13919
                     /locus_tag="VYQ30_000084"
     CDS             12933..13919
                     /locus_tag="VYQ30_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1965260.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13935..15368
                     /locus_tag="VYQ30_000085"
     CDS             13935..15368
                     /locus_tag="VYQ30_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1965261.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15397..16431
                     /locus_tag="VYQ30_000086"
     CDS             15397..16431
                     /locus_tag="VYQ30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1965262.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16631..17368
                     /gene="mecA"
                     /locus_tag="VYQ30_000087"
     CDS             16631..17368
                     /gene="mecA"
                     /locus_tag="VYQ30_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1965263.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17519..18805
                     /locus_tag="VYQ30_000088"
     CDS             17519..18805
                     /locus_tag="VYQ30_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1965264.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18807..19676
                     /locus_tag="VYQ30_000089"
     CDS             18807..19676
                     /locus_tag="VYQ30_000089"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1965265.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19752..19829
                     /locus_tag="VYQ30_000090"
                     /pseudo
     CDS             19752..19829
                     /locus_tag="VYQ30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            19971..20909
                     /gene="msrB"
                     /locus_tag="VYQ30_000091"
     CDS             19971..20909
                     /gene="msrB"
                     /locus_tag="VYQ30_000091"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1965266.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20967..22691
                     /locus_tag="VYQ30_000092"
     CDS             20967..22691
                     /locus_tag="VYQ30_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965267.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22693..24441
                     /locus_tag="VYQ30_000093"
     CDS             22693..24441
                     /locus_tag="VYQ30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965268.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24743..26002
                     /locus_tag="VYQ30_000094"
     CDS             24743..26002
                     /locus_tag="VYQ30_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1965269.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26078..26213
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26252..26752
                     /locus_tag="VYQ30_000095"
     CDS             26252..26752
                     /locus_tag="VYQ30_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1965270.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26828..27196
                     /gene="rplL"
                     /locus_tag="VYQ30_000096"
     CDS             26828..27196
                     /gene="rplL"
                     /locus_tag="VYQ30_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1965271.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27398..27571
                     /locus_tag="VYQ30_000097"
     CDS             27398..27571
                     /locus_tag="VYQ30_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965272.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAIGLMAELEKSGLVRLES
                     SGDLILKELGGAL"
     gene            27568..28347
                     /locus_tag="VYQ30_000098"
     CDS             27568..28347
                     /locus_tag="VYQ30_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1965273.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKILKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVASSNWASVKKSLGQDENETAPVSHSAPSDTEVSETKYSE
                     TDSLFIEDEEERNTQPILKRKVEKVTKYDRDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            28447..29805
                     /gene="dcm"
                     /locus_tag="VYQ30_000099"
     CDS             28447..29805
                     /gene="dcm"
                     /locus_tag="VYQ30_000099"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV1965274.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARESYKSIFQT
                     EGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPQNRE
                     RVFIIGHSRKKGTRLLFPFRREGQATNPETLKALGNLNPSRSGMSGKVYYSEGLAPTL
                     VRGKVEGFKVAIPCMTPDRLEKRQNGRRFKDNQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPGSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGILINGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     VK"
     gene            29789..30238
                     /locus_tag="VYQ30_000100"
     CDS             29789..30238
                     /locus_tag="VYQ30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965275.1"
                     /translation="MKALNKESILYCDELETELHDAEMMQLDEQIFLMPNYPCEFEVK
                     FLDYYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFVDDNQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKPINFSNLLDSLIVSEYQMEPNLWEVSHD"
     gene            30231..30611
                     /locus_tag="VYQ30_000101"
     CDS             30231..30611
                     /locus_tag="VYQ30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965276.1"
                     /translation="MIDLYLSKNNHRNQLLLDFFQNYGIEVSCHSVSEMTKEKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            30624..30857
                     /locus_tag="VYQ30_000102"
     CDS             30624..30857
                     /locus_tag="VYQ30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1965277.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKVEQRMPQGPWVERIVQVLDLDYEDLFRR"
     gene            30860..31447
                     /locus_tag="VYQ30_000103"
     CDS             30860..31447
                     /locus_tag="VYQ30_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1965278.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKKVHKVSMKSRMKWLGIGLASLLIISLCFSLIHAQGSTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRIALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            31649..31744
                     /locus_tag="VYQ30_000104"
                     /pseudo
     CDS             31649..31744
                     /locus_tag="VYQ30_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
BASE COUNT         9546 a         5756 c         7104 g         9338 t
ORIGIN      
        1 gcttttctat aaaatcaaat tttccagata tctactgaag ctgatttatt ttttcatctt
       61 tcttctacta cctttgtcac taaatttgac agacacatat tgctgataca acctctctac
      121 ctataatctc acagttaacc tgaggctagt ttcatagttt gctatttgat tttcgttgat
      181 aaaaagaaat gctcctccaa gacggaagag cattttttat tgtgattctt acacatttac
      241 ttcctagttt tgcaggaagt ctagtatacc attttttaaa agaagagtaa aaaagcaagg
      301 gtagtttctt ttttgagaat tgtctaaaag tttgatataa tggtagttat gaatagaata
      361 agagtcagca aaagggttga aaagaagctt gctaaggggc tagttttact agaagccagt
      421 gatcttgaga atgtcaatct taaggatcag gaagtagagg tgcagggtca ggaaggaaac
      481 tttcttggga ctgcctacct ttctcagcaa aacaagggct tgggctggtt tgtcagcaaa
      541 gacaaggtgg ccttcaatca agctttcttt gaaacgttgt ttagaaaagc caaagaaaag
      601 agaagcgcct actatcaaga tgatttaaca actgcctttc gtctctttaa tcaagaggga
      661 gatggctttg ggggtctgac agtggacctt tatggcgact acgccgtctt ttcttggtat
      721 aactcttatg tttatcagat tcgtcagact atatcagaag cctttagaca ggttttccct
      781 gaggttttag gggcttatga gaaaatccgc tttaagggtt ttgactatga atctgcccat
      841 gtttatggtc aagaagcacc tgactttttc actgttttag aaaatggtgt cctgtatcaa
      901 gtctttatga atgatggctt gatgacagga attttcctag accagcatga ggttcgcggt
      961 agtttagttg acggcttggc tatgggtaaa tccttgctca atatgttttc ctacacagcg
     1021 gctttttcag tagctgcggc catgggagga gctagccaga caacttctgt tgatctagcc
     1081 aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa atgggctcag cacagacgag
     1141 catcgtttta tagtcatgga tgtctttgag tatttcaaat atgccaaacg caaagacttg
     1201 acctacgatg tgattgtcct agatccgcct agctttgctc ggaataaaaa acaaactttc
     1261 tctgtggcca aggattatca caagttgatt tcccagagtc ttgagatttt aaatccggga
     1321 gggattatca ttgccagtac caatgctgcc aatgtttccc gccagaaatt tacagaacaa
     1381 attgataaag gctttgcagg aagaagttac cagattttaa acaaatatgg tcttccagca
     1441 gattttgcct ataataaaaa agatgaaagt agtaattacc tcaaggtgat tagtatgaag
     1501 gttagtaaat gaaattaatc gtttcagtaa tgccaagaag tttagaggag gctcaggctc
     1561 tggatgccac gaggtacctg gatgccgaca tcattgaatg gcgtgccgac tatctgccta
     1621 aagaagcgat tttgcaggta gctccagcca tttttgaaaa attcgcaggc cgtgagttgg
     1681 ttttcacgct acgaactcgc tccgaaggtg gagaaatcga cctttctcca gaagagtata
     1741 tccatctaat caaggaagtt gcgcaactct atcaaccaga ctatattgat tttgagtact
     1801 atagctacaa ggatgttttt gaggaaatgc tgaacttccc aaatcttgtt ttgagttacc
     1861 ataatttcca agaaacacct gagaacatga tggaaatctt gtcagagttg acgatcctaa
     1921 atccaaaact tgttaaggtt gcggtgatgg ctcacacgga gcaggatgtc ttagacttga
     1981 tgaactatac acgaggcttt aaaaccctca atcctgaaca ggaatatgtg accatttcta
     2041 tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt gactggttcg agttggtctt
     2101 ttgccagtct ggatgaggtt agtgcccctg ggcagatttc tctagctagc atgaaaaaaa
     2161 tcagggagat tttggatgaa gcttgatagc tatacacgtt tagctgcagt tgttgccaat
     2221 cctattaagc attctatttc tcccttcatc cacaatagag cctttgaggc gacagctacc
     2281 aacgctgctt acgtggcttg ggagattgaa gcgagtgact tggcagaaac agtggccaat
     2341 attcgtcgct accagatgtt tggcatcaat ctgtccatgc cctataagga gcaggtgatt
     2401 ccttatttgg atgagctgag cgatgaagcg cgcttgattg gtgcggttaa tacggttgtc
     2461 aatgagaatg gcaatttaat tggatataat acagatggca agggattttt taagtgcttg
     2521 ccttctttta caatttcagg taaaaagatg accctgctgg gtgcaggtgg tgcggctaaa
     2581 tcaatcttgg cacaggctat tttggatggc gtcagtcaga tttcggtctt tgttcgttcc
     2641 gtttctatgg aaaaaacaag accttaccta gacaagttac aggatcagac aggttttaaa
     2701 gtggatttgt gtgctttaga atatgtttct gaactgcaag caaggattgc cgagtcggat
     2761 ttgctagtta atgccaccag tgtgggcatg gatggccaat cctctcctgt tcctgaaaac
     2821 atagtcttac cagaaactct tttagttgca gatatcatat accaaccctt tgaaacaccg
     2881 tttttgaaat gggctagaag tcagggcaat ccagcagtca atggtctggg aatgttgctc
     2941 tatcaagctg cagaagcttt tcaactgtgg acaggcaagg aaatgccgac agaagagatt
     3001 tggcagtctt taacagaaaa ataccaataa agaaagggag actctcctat gaaaatcaga
     3061 atcgatattc ctcaccatcc ttatgatatt cagattgaaa aaggttgtat ggcccaggct
     3121 ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag tcattgtgac agataaccat
     3181 gtagcctctc tctatgcaga gaaggtcaag ctcagcctag aagatgctgg ttttcaggta
     3241 gctgtttttg atttcttaga aggtgaagaa agaaagaatt taactactgt tcagaaagtc
     3301 tatgaatttc tagtcaagca aggtctgact cgtagcgatg gaatcgttgc tcttggtggt
     3361 ggcgttgttg gggacctggc tggatttgta gcctctacct atatgcgggg tattcacttt
     3421 gttcagattc cgactagttt gacagctcag gttgattctt ctatcggtgg aaagacaggc
     3481 gttaacacac catttgctaa aaatatggtg gggacctttg cccaaccaga tggggttttg
     3541 attgatccac ttgttcttga aaccctcgga aaaagagagt tgattgaagg gatgggtgaa
     3601 gttatcaagt atggcttgat tgaggatcca gaactgtggg ctctcttgac gggactgaat
     3661 ggttctgttg agagtatttt ggaacatgca gagaccttga ttgaacattc ttgtcaggtg
     3721 aagcgcaaga tggtggttga agatgagttg gacaatggta ttcgtcttta cctcaatttt
     3781 ggccacacta ttggccatgc catcgaagcg actgccggtt atggcaaggt catgcatgga
     3841 gaggctgttg ccatgggaat ggtacagatt tccaaggttg ctgaggaaaa aggcctcatg
     3901 ccagctggca ttacccaatc tatcacagag atgtgtcaga aattcggctt gcctgttgac
     3961 tatgaaaatt gggaagttga caagctttat caggctctta ctcatgacaa gaaagcgcgt
     4021 ggtaacacct tgaaattggt cttggtgcca gagcttggtt cagcgatcat tcacccagtt
     4081 tctctggaag agatgaaaga ctacttggta aaataaggag agtgtatgag atatttaact
     4141 gcaggagaat cacacggccc ccgtctaaca gctattattg agggaattcc agctggactt
     4201 ccattgacag ctgaggatat caatgaggac cttagacgcc gtcagggtgg ctacggtcgt
     4261 ggtggtcgta tgaagattga gagtgaccag gttgtcttta cttcgggcgt tcgccacggg
     4321 aagacgacag gggcgcctat tactatggat gtcatcaata aggatcacca gaaatggctg
     4381 gacatcatgt ctgcggagga cattgaagac cgccttaaaa gcaagcggaa aattactcat
     4441 cctcgcccag gtcatgccga tttggttggg gggattaagt accgttttga tgatttgcga
     4501 aattctttgg agcgttcatc agctcgtgaa accaccatgc gggtggcagt tggtgcagta
     4561 gccaaacgcc tcttggctga gctggatatg gagattgcca accatgtcgt ggtctttggt
     4621 ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg aaattaagca aagagctgcc
     4681 cagtctgaag tttctattgt caaccaagaa cgagaacagg aaatcaagga ctatattgac
     4741 caaatcaaac gtgatggtga taccatcggt ggggttgtgg agacagtcgt cggaggcgtt
     4801 ccagttggtc ttggttccta tgtccaatgg gatagaaaat tggatgcaag attggctcaa
     4861 gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg gtcttggctt tgaggctggt
     4921 tatcgtaaag gcagccaagt tatggatgaa attctctggt ctaaagaaga cggttatact
     4981 cgccgtacca ataatctagg tggttttgaa ggtggtatga ccaatgggca acccatcgtt
     5041 gttcgtgggg tcatgaaacc cattcctact ctttataaac ctcttatgag tgtggatatc
     5101 gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg atccgactgc tcttccagct
     5161 gcaggaatgg tcatggaagc agttgtagca acggttctgg cgcaagaaat cctcgaaaaa
     5221 ttctcatcag ataatcttga ggaactaaaa gaagcggtag ccaaacaccg agactataca
     5281 aagaactatt aaggagttcc tatggcaaaa acaatctata tcgcaggtct tgggttgatt
     5341 ggagcctcta tggcacttgg tatcaaacgc gatcatccag attatgaaat tttaggttat
     5401 aatcgtagtc aagcttcgag agatatcgcc ttgaaagaag gcatgattga ccgtgcaacg
     5461 gatgattttg ctagttttgc tcctttggca gatgtcatta tactcagctt gccaatcaaa
     5521 caaactattg ctttcattaa ggagttggcc aatttggatt tgcgagaagg cgttattatt
     5581 tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg cggagcagta tttggctggc
     5641 aagtctgttc gctttgtcgg ggcccatccc atggctggta gtcacaagac aggggctgct
     5701 tcggcagatg tcaatctttt tgaaaatgcc tattatatct ttacaccttc aagcctgaca
     5761 agtcaggaca cgcttaagga aatgaaggat ctgctttcag gtcttcatgc tcgttttatc
     5821 gagattgatg ccaaggagca tgatcgtgtc acttctcaga ttagccattt tcctcatatt
     5881 ttggcttcta gtctcatgga gcagactgcg gtctatgctc aagagcatga gatggcaagg
     5941 cgctttgcgg caggtggttt tcgagatatg acccgaattg cggaaagcga gccaggaatg
     6001 tggacctcca ttctcttgtc caatagcgag accattctgg atagaattga ggatttcaag
     6061 gaacgtttgg aagtgattgg tcaggccatt agtaagggag atgaagagca aatttggaac
     6121 ttttttaacc aagcgcgtga acaacgccag accatggaaa tccataagcg tggtggtgtg
     6181 gatagctctt acgacctcta tgttgacgtt cctgatgaag aagatgtcat cctgcggatt
     6241 ttggaactgc tacgtggaac ttccttggtt aacatccaca tcaacgagga aaatcgtgag
     6301 gatattcacg gaatcctaca aatttcattt aaaaatgctc aagacttgga aagagctgag
     6361 catctcataa cagaaaatac cgactataca gtcgtcatca aataaggaga agaacatgtc
     6421 aaatatttac gatagtgcaa acgaactcag tcgcggtcta cgcggattac cagaatacaa
     6481 ggctgttaaa gcagctaaag atgcgattgc agcagatgct gaggcaagca aaatctttac
     6541 agaatatctt gcctttcaag aggaaattca aaaactagca cagacaggtc aaatgccaga
     6601 tgcgtccttc caagctaaga tggaaggctt tggtaaacag attcaaggaa atagcctctt
     6661 gtcagaattc tttaccaagc aacaacaatt ggccatttac ctttctgaca ttgaaaaaat
     6721 tgtttttgaa ccagtttcag aattgctgaa ataagaaaag aacgatccta aagtcaggaa
     6781 tttttaagga atttctggct ttttatgata aaataggtaa aagtattgca agtatgaggt
     6841 ccagtatgaa actaaaaaca aacattcgcc atttacatgg tagtatccgc gtcccaggtg
     6901 acaagtctat cagccaccgt tccattatct ttggaagttt ggctgagggt gagaccaagg
     6961 tttatgatat tctgcgaggt gaagacgttc tttcgaccat gcaggttttt cgtgaccttg
     7021 gtgttgaaat tgaggataaa gatggggtta ttaccattca aggtgtaggc atggctggct
     7081 taaaagcgcc acaaaatgcc cttaatatgg gaaattctgg cacctcgatt cgcctgattt
     7141 caggtgtcct tgctggtgca gatttcgaag tagagatgtt tggagatgat agtctttcca
     7201 aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat gggcgtcagc atttcagggc
     7261 aaactgaacg agacttgcct ccccttcgct taaaagggac gaaaaaccta agacctattc
     7321 attatgagtt gccaatttcc tctgcccaag tcaagtcagc cttgatgttt gcagccttac
     7381 aagctaaggg ggagtcagtt attatcgaaa aagagtgcac ccgtaatcat actgaagata
     7441 tgttgaaaca atttggtggt catttaagtg tggacggtaa gaaaatcaca gtccaagggc
     7501 cacaaaaatt gacaggacag aaggtggtcg taccaggaga tatttccagt gcagcctttt
     7561 ggttagtcgc aggtttgatt gttccaaatt ctcgtctagt gctgcagaat gtggggatca
     7621 atgaaactcg caccggtatt attgatgtca ttcgtgccat gggtggaaaa ttggaaataa
     7681 ctgaaatcga tccagtcgct aaatctgcaa ccttgattgt tgagtcttct gacttgaaag
     7741 gaacagagat ttgtggcgct ttgattccac gtttgattga tgaattgcct attattgccc
     7801 tacttgcgac ccaagcccaa ggtgtaacag ttatcaagga tgctgaggag ctcaaggtca
     7861 aggaaacaga ccgtattcag gttgtggcag acgccttaaa tagtatggga gcagatatta
     7921 ctcctacggc agatgggatg attatcaaag gaaaatcagc tcttcacggt gctagagtca
     7981 atacgtttgg tgaccaccgt atcggcatga tgacagctat cgcagcccta ttggttgcag
     8041 atggagaggt ggagcttgac cgtgcagaag ccatcaatac cagctatcct agtttctttg
     8101 atgatttgga gagcttgatt catggctaag gtgttattag ggtttatggg ggctggaaaa
     8161 tcgactattg caagaggctt ggaccctaat taccttgata tggatgctct gattgagaag
     8221 cgcctaggta tgtccattgc gaattttttc gctgaaaagg gagaagcagc ctttcgtcag
     8281 gtagaatcag aagtcctagc tgatttacta caaacagacc aagtcgtgtc aactggagga
     8341 ggagtggtta tttctcagag aaatcgtgac ttactcaaga ctaatacaga taacatctac
     8401 ctgaaagcag attttgaaac cctctaccaa cgtatcgcag ctgataagga caatcagcga
     8461 ccgctttttc taaataatag caaggaagaa ctagtagcta tttttcaaga aagacaggct
     8521 tggtatgagg aagtggctag tcgggttttg gatgtgacca agctaagccc agaggaaatt
     8581 atagaggaac taagatgaaa attgcttatc taggtcccaa aggatcattt tcacaccacg
     8641 ttgtgcagac agcttttcct catgaggaat tgcaggcttt tgccaacatt acagatgtca
     8701 tcaaggctta tgagcaagga ttagtggact attctgtggt gccagttgaa aattctattg
     8761 agggtagtgt tcatgaaacc ttggactatc tttttcatca ggctcacatc caagcagtag
     8821 cagaaatcgt tcagcctatt catcagcagt tgatggtggt tccaggccat actaagattg
     8881 aaaagatttt ttcacatcca caggccttgg ctcaaggaaa gaaattcatc gatgaacaat
     8941 atccagaggc tcaaatcgag gtaacagcta gtacagctta tgcggcccgt tttatttccg
     9001 aacatccaga ccagcccttt gcagcagttg cacctagaag ttctgctgaa gaatatggat
     9061 tggaactgat tgccgaggat attcaggaaa tggcagccaa tttcacacgt ttctgggttc
     9121 taggagctga aaagcctagt attcccttgc aagcacaaac tgaaaagatg agtttggcct
     9181 tgacattacc tgacaacctt ccaggtgcac tttataaggc cctgtcgacc tttgcttggc
     9241 gaggaattga cttgacaaaa attgaaagtc gtccactcaa gacagcactg ggtgaatact
     9301 ttttcattat cgatgtggat tataccgata aggacttggt ccactttgcc caaaaagaat
     9361 tagaagcgat tggaatccag tataaaattc tgggtgccta tcctatttat ccaatatcag
     9421 accatggaaa ggagagaaga tgagtaaaga aaatccctta agtcatcatg agcagttgcg
     9481 ttatgattat ttgctaaaaa atattcacta tctcaatgag agagaaaaaa atgagtttgc
     9541 ctatttgcaa gaaaagctaa ctcttgctag gggaaatagt agctctagct tggaacaaga
     9601 aagagaagag caggttgact taccaagcta tgcgaaccgg agtcgctcac aatccaaatc
     9661 acaagcactc tctttccctc caaaaaagaa aagacggaag ctccgtctta aacgaatttt
     9721 tatggtgatt ttttcacttt tggtctgtgt ggctttggcc atggtattca tgtttttgcg
     9781 tggttaccaa gatgctagcg caaagaaaac tgctgatgcc cgggcagctc aagtagaagt
     9841 ctttaatggt caggacacta gagatggagt taatatttta atcatgggta ctgatggtcg
     9901 aatcggccag aacagtgttg agacacgaac tgactctatt atggtattaa atgtcggggg
     9961 ctcagataag aaaatgaagc tagtcagttt catgcgtgat aatttggtct atatagatgg
    10021 ttatagtcaa gtgattaatg gtagaaaaca gacagataac aagttaaacg tagcctacga
    10081 gttaggagaa caagaggggc aaaaaggggc agaaatggtt cgccaagtct tgaaagataa
    10141 ttttgacttg gacattaagt actacgcctt ggttgatttc caggcctttg ccacagcgat
    10201 tgacacgctt ttccctgatg gggtgacgat tgatgctcaa ttttcaacat tgaatgggcg
    10261 tcccctaaca gaagctacag tcggagatga tttatacgct accgagactg agtctccaac
    10321 ccaaaccatt aaagtcggaa aacagcagat gaatggctcg accctgctca attatgctcg
    10381 tttccgtgat gatgatgagg cggattacgg ccgtacaaaa agacagcaac aagttttaac
    10441 agcaatttta gagcaaatta aagatcccac taaacttttc actggttctg aagctcttgg
    10501 aaaggttttt gctatgacct caaccaacgt accctatact ttcctcctaa caaacggttt
    10561 atctgttttg gatggagcaa aaaatggtat tgaaaaattg acgattccag aactgggtga
    10621 ctgggtagat gcctatgatg tttatggggg cttgggcctg ctggttgatc aaaacaaata
    10681 tcaaaccaag ctcgctcaaa tgggcttaag ataagataat actcaataaa aatcaaagtg
    10741 caaactagga agctagccgc aggctgctaa aaacacagtt ttgaggttgt agataagact
    10801 gacgaagtca gttacctata cctacggcaa ggtgaagctg acgtggtttg aagagatttt
    10861 cgaagagtat aagacagaaa aatcaaagtt tgatagctat ttggatagca gtcaggcttt
    10921 gattttttac agtctgcaat agattttcac cccttgtttg tggtataatg aaacgatacg
    10981 atagaaagaa taatgaacaa tatgactgat ttaaaagcaa ttcaggctcg tagtctggag
    11041 atggctgaat attttgtggc cttttgtaaa gaacatgatt tgctctgtta tctctgtggc
    11101 ggaggcgcta ttggtgccct tcgaaacaag ggatttattc cttgggacga cgacctagac
    11161 ttttttatgc ctcgtaaaga ttatgagaaa ttagcagaat tatggcctcg ttatgcagat
    11221 gaacgttatt tcttgtcaaa gagtcacaag gattttgttg atcgtaatct ttttattacc
    11281 attcgtgaca aggaaaccac ctgtatcaag ccttatcagc aggatttgga tttgccacat
    11341 ggtctggcct tggatatttt gcctttggat tattatccga aaaatccagc tgagcggaaa
    11401 aaacaggttc gttgggcctt gatttattca ctcttttgtg cgcaaactat tccagaaaag
    11461 catggtgctc tcatgaaatg gggaagtcgc attttactgg gtttgactcc aaaatctctc
    11521 cgttatcgca tctggaaaaa agctgagaaa gaaatgacta agtatgattt ggctgattgt
    11581 gatggcatta cagaattatg ctcaggtcct ggctacatga gaaacaagta cccaatcaca
    11641 tcttttgaag acaatctttt cttgccattt gaaggaacag agatgcctat tccaatcggc
    11701 tatgatgtct atctcagaac tgcttttggg gattatatga cgcctccacc agcagacaag
    11761 caggtaccgc atcatgatgc tgtcatcgct gatatggata agtcttatac agaatacaag
    11821 ggagaatatg gtggctaaga aaaaaatctt attttttatg tggtcttttt ctcttggagg
    11881 tggtgcagag aagattctat caaccattgt ttcaaatctg gatccagaaa agtatgatat
    11941 tgatattctt gaaatggagc actttgacaa gggatatgaa tctgttccaa agcatgtacg
    12001 cattttaaaa tcccttcaag attatcgcca aaccagatgg ttacgagctt ttttgtggag
    12061 aatgagaatt tattttccaa gactggctcg tcgtttgctt gtaaaggatg attatgatgt
    12121 tgaagtttct tttaccatta tgaatccacc actgttgttc tctaaaagaa gagaagtcaa
    12181 gaagatatct tggattcatg gaagtattga agaacttctt aaggatagct ctaaaagaga
    12241 atcacataga agccagttgg atgctgcgaa tacaattgta gggatttcaa aaaagaccag
    12301 caattctatc aaggaagttt atccagatta tacttctaaa ttacagacaa tctacaatgg
    12361 atatgatttt cagactattc tagaaaaatc tcaagagaag atcgatatcg agattgctcc
    12421 tcaaagtatc tgtactatcg gacggattga ggaaaataag ggttctgacc gtgtagtgga
    12481 agtgatacga ttattacacc aagagggaaa aaactatcat ctctatttta tcggggctgg
    12541 tgatatggaa gaggaactga aaaaacgagt caaagagtat gggattgagg actatgtaca
    12601 tttccttggt tatcaaaaaa atccttatca gtatctatct cagacgaaag ttcttttgtc
    12661 tatgtctaaa caagaaggtt ttcctggagt gtatgtggag gccttgagtc tgggactccc
    12721 ttttatctct acggacgttg gaggggctga ggaattatcc caagaaggac gatttggaca
    12781 aatcattgag agcaatcaag aggcagctca ggcgattact aattacatga cttctgcctc
    12841 aaactttgat gtcgatgagg ctagccaatt cattcaacaa tttacaatta caaaacaaat
    12901 cgaacaagta gaaaaactat tagaggagta gcatggaaac tgcattaatt agtgtgattg
    12961 tgccagtcta taatgtggcg cagtacctag aaaaatcgat agcttccatt cagaagcaga
    13021 cctatcaaaa tctggaaatt attcttgttg atgatggtgc aacagatgaa agtggtcgct
    13081 tgtgtgattc aatcgctgaa caagatgaca gggtgtcagt gcttcataaa aagaacgaag
    13141 gattgtcgca agcacgaaat gatgggatga agcaggctca cggggattat ctgattttta
    13201 ttgactcaga tgattatatc catccagaaa tgattcagag cttatatgag caattagttc
    13261 aagaagatgc ggatgtttcg agctgtggtg tcatgaatgt ctatgctaat gatgaaagcc
    13321 cacagtcagc caatcaggat gactattttg tctgtgattc tcaaacattt ctaaaggaat
    13381 acctcatagg tgaaaaaata cctgggacga tttgcaataa gctaatcaag agacagattg
    13441 caactgccct atcctttcct aaggggttga tttacgaaga tgcctattac cattttgatt
    13501 taatcaagtt ggccaagaag tatgtggtta atactaaacc ctattattac tatttccata
    13561 gaggggatag tattacgacc aaaccctatg cagagaagga tttagcctat attgatatct
    13621 accaaaagtt ttataatgaa gttgtgaaaa actatcctga cttgaaagag gtcgcttttt
    13681 tcagattggc ctatgcccac ttctttattc tggataagat gttgctagat gatcagtata
    13741 aacagtttga agcctattct cagattcatc gttttttaaa aggccatgcc tttgctattt
    13801 ctaggaatcc aattttccgt aaggggagaa gaattagtgc tttggcccta ttcataaata
    13861 tttccttata tcgattctta ttactgaaaa atattgaaaa atctaaaaaa ttacattaga
    13921 acgggggtga ccaagtgatt gatgggaaac gattattatt tagtttgacc atagtcagtt
    13981 atgccttgac gctagtaagt ggaattgtgt atctgtttaa taataataat gttagcttac
    14041 tttctacttt attgttcttg ttggttagta gcttaattgc ttgttggaat gatatcaagt
    14101 attacttaat ccattttatt ttctatttaa ccatttttgt gtttctggta tcaagaccga
    14161 ccattgatta ttttagggat ggtgctttgg atacctatca tccaatagcc tatcgttttg
    14221 cctttatagt tgtcatgatt tcgattctgg gcttgaccac aggaggcatt ctggctcgtt
    14281 acttcatagc taggaagaaa ataaaagtag caaatatagg aaattctcta aaagaggttt
    14341 atatcaagcg gttacgcttt gtatcactag gagtttttct tctaacttat cctttctatt
    14401 tcattcggtt atttgaacgg ctcttgtatc gtttgcagac ttcctactat gcctactatg
    14461 caaattttga aagtaaactg ccttatttta cctacatttt gtctaccttt acggtctatg
    14521 caatgtgtat gtatctggca accaagccaa agaaattgca ggccacagca gtgcttgtct
    14581 cctttattgc agctaacact attcatttgg caattgggac acgaaatccc tttattttaa
    14641 gtattttatt tgcttttgtt tattacttta tgcgggagca agctgaaaaa ggaaaatgga
    14701 ttgggtttaa agaaaagtta gcgatttttg taggttctcc tattctcatg ttagcgatgg
    14761 gagtactcaa ttatgtacgg gataatgtcc aagtttccca tacaggtttc tgggatatct
    14821 tacttgactt tatctataaa caagggacta gttttggtgt tctggctcga ggttttctat
    14881 ttaacagtag cctcccttac cgagatttcc gtaattttac ttttggtcct gttcttgatt
    14941 attttgcaag ggggagtttg ggagccattt tcggaggaaa agcctttgaa catacaacca
    15001 atagtgtgga actagctatt gatagtaata gttatgccca caatctatcc tatcttgtct
    15061 tgaacaagga atacttgaaa gggcatggta tcggaagtag ttatatcatg gagttgtata
    15121 ccgactatgg tatgattgga gtctttctgc ttagtttctt actcggcgta ttatttatag
    15181 ccatgctgca agtagcctat cgctcaagga caatcctatt tgctttatcc ctactcatct
    15241 tgaataatct attctttatg ccaagaagca gcttttcaga aagtttcttc aatttattta
    15301 caatgcaatt ctggggaatt gttcttgtga ttatatttgt agcaaaaatg cttacaaagg
    15361 aaaactagta tctactaaac aaaggagaaa aaaatcatgt ttgaacatta ttcagtagct
    15421 gatttgtttg caaatcttta taaaaagcga aaagcaaata ttttagctct catcgcttta
    15481 tttgctctca ttgctgtacc atttacaatt aaagcagtta ggaataaaaa cactgtcaaa
    15541 gatacaacaa gttattcaac ttatcttatc tataaaatca ctcctccaaa agagtcggcc
    15601 aaaacgattt tgaatcatca aattggtggt tatagtgatt tttatgggaa attgattgat
    15661 ggtaatttga atggagctta tcttttcaat gatgtagaac ccagtgagtt gaaaaaaatt
    15721 gccagtgaat tagacacgac agaaacaacc ttgaaaaatt caacgaatga ctattggtgg
    15781 aaaaaattga ccgtctacta tatgattgac gatgcagggg ttggtgtgaa aattttgaca
    15841 tcaagtaaag atgccaatga cttgttagag aaaaaaattg atgggttgat tgagaaattt
    15901 aaacatgcct atgcaaatgt gaaaattgaa aaactggaaa ccatcaactc taaagaattg
    15961 aacgcaaatg gtgaaacagc gcttggctta aatgtgaaaa atctgattct tcgtttagtt
    16021 gttattggag tggtttgtgt gattttggtt gtgatgggaa atgtgttagt ttatctcttt
    16081 aatccaacaa tcaatagagt aggtgatttt tctcagtatc aaattgattt tgtaacagag
    16141 attacaacaa ttgctaacct agcagatgtt ttgtcataca aaaatactgg acaggaattg
    16201 accatcgtta gctcaaataa agctatccta gataaattga aacagagtca agaagcttta
    16261 aaaggaatgc attttgtaga tttacaggat gtatcatctc ttttggaaag agatacagtc
    16321 cttcttgttg aagagtacgg agtgactcgt tataagaaat ttgagcaaag tcttcaaatt
    16381 ctcagaaact taaatcgttc tatccttggt gtagcaacct ttaaattata agtattctga
    16441 tgtataaggt cttcaaaatc ggctaagagc tgattttgaa ggctttttct atctgtctgc
    16501 agaaattttc ttttggagtc tcctatagaa agaccctagg gaaaatctat ccgtatagag
    16561 gagccttctt ttgataaaat cgtaaaaatc tagtataata gatagaactg aaagtatgag
    16621 gttactagca atgaaaatga aacaaattag tgatacaact ttaaaaatca cgatgtcttt
    16681 agaggatttg atggatcgtg gaatggagat tgctgacttt ctcgttcctc aagaaaaaac
    16741 agaagagttc ttttatgcta tcttggatga gctagagatg cctgatagct ttctggatac
    16801 aggcatgttg agcttccgtg tgactccaaa acctgataag gtagatgtct ttgtgaccaa
    16861 gtcaaagatt gaccaaaatc tagattttga agacttatcg gatttgccag atatggaaga
    16921 attggctcaa atgtctccag atgaatttat caaaaccctg gaaaaaagca tcgcagacaa
    16981 aaccaaggat gatatcgaag ccattcaatc tcttgagcaa gttgaagcca aggaagaaga
    17041 acaagagcag gctgaacaag aagctgagag taagaaagaa ccttacatct actacatcct
    17101 ttcttttgct aagttggctg acttggtagc ttttgccaag acagtgactt ttgaaatgga
    17161 aacttctgaa ctctacaaaa tgaacgagcg ctattatttg accattttag tggatattga
    17221 aaatcatcca agcccatatc cagcttggct gttggcccgt atgcgcgagt ttgcagacga
    17281 tagtgatatc agtcgctcag tcttacaaga gtatggtcaa gtcttgatga gtcacgatgc
    17341 agtgctcaat ctgcaaaaaa tcggctaatc ttttctggaa agctattttc agattttaag
    17401 aagagcgaat cgtctgattc gttttttctt tttatactga aatagtgatt tactataata
    17461 ggaattttca caaaattctg ttataatggc tatattagaa aatttcgagg agataaagat
    17521 gacagttaaa attgctttac taggatttgg taccgttgca agtggtgtgc ctttcctcct
    17581 aaaggaaaat ggaggaaaaa tcaatcaatc agcacattca gatatcaaag ttgctaaggt
    17641 attggtcaag gatgaagatg aaaaaaatcg cttgcttgca gcagggaatg actttaactt
    17701 tgtaaccaat gtggatgata ttttatcaga ccaggatatt actatcgtag tggaattgat
    17761 ggggcgtatt gagcctgcta aaacctttat cactcgtgcc ttggaagctg gaaaacacgt
    17821 tgttactgct aacaaggacc ttttagctgt ccatggcgca gaattgctag aaatcgctca
    17881 agctaacaag gtagcacttt actacgaagc agcagttgct ggtgggattc caattcttcg
    17941 tactttagca aattccttgg cttctgataa aattacgcgc gtgcttggag tagtcaacgg
    18001 aacttccaac ttcatggtga ccaagatggt ggaagaaggc tggtcttacg atgatgctct
    18061 tgcggaagca caacgtctag gatttgcaga aagcgatccg acgaatgacg tagatgggat
    18121 tgatgcagcc tacaagatgg ttattttgag ccaatttgcc tttggcatga agattgcctt
    18181 tgatgatgta gcccacaagg gaatccgcaa tatcacacca gaagacgtag ctgtagctca
    18241 agagtttggt tacgtagtga aattggttgg ttctattgag gaaacttctt caggtattgc
    18301 tgcagaagtg actccaacct tcctacctaa agcgcaccca cttgctagtg tgaatggcgt
    18361 aatgaacgct gtctttgtag aatctatcgg tattggtgag tctatgtact acggaccagg
    18421 tgcgggtcaa aaaccaactg caacaagtgt tgtagctgat attgtccgta tcgttcgtcg
    18481 tttgaatgat ggtaccattg gcaaagactt caacgaatat agccgtgact tggtcttggc
    18541 aaatcctgaa gatgtcaaag caaactacta tttctcaatc ttggctccag actcaaaagg
    18601 tcaggtcttg aagttggctg aaatcttcaa tgctcaagat atttccttta agcaaatcct
    18661 tcaagatggc aaagagggtg acaaggcgcg tgtcgttatc atcacacaca agattaataa
    18721 agcccagctt gaaaatgtct cagctgaatt gaagaaggtt tcagaattcg acctcttgaa
    18781 taccttcaag gtgctaggag aataagatga agattattgt acctgcaacc agtgccaata
    18841 tcgggccagg ttttgactcg gtcggtgtag ctgtaaccaa gtatcttcaa attgaggtct
    18901 gcgaagaacg agatgagtgg ctgattgaac accagattgg caaatggatt ccacatgatg
    18961 agcgtaatct cttgctcaaa atcgctttgc aaattgtacc agacttgcaa ccaagacgct
    19021 tgaaaatgac cagtgatgtc cctttggcgc gcggtttggg ttcttccagc tcggttatcg
    19081 ttgctgggat tgaactagcc aaccaactgg gtcaactcaa cttatcagac catgaaaaat
    19141 tgcagttagc gaccaagatt gaagggcatc ctgacaatgt ggctccagcc atttatggta
    19201 atctcgttat tgcaagttct gttgaagggc aagtctctgc tatcgtagca gactttccag
    19261 agtgtgattt tctagcttac attccaaact atgaattacg tactcgcgac agccgtagtg
    19321 tcttgcctaa aaaattgtct tataaggaag ctgttgctgc aagttctatc gccaatgtag
    19381 cggttgctgc cttgttggca ggagacatgg tgaccgctgg gcaagcaatc gagggagacc
    19441 tcttccatga gcgttatcgt caggacttgg taagagaatt tgcgatgatt aagcaagtga
    19501 ccaaagaaaa tggggcctat gcaacctacc tttctggtgc tgggccgaca gttatggttc
    19561 tggcttctca tgacaagatg ccaacaatta aggcagaatt ggaaaagcaa cctttcaaag
    19621 gaaaactgca tgacttgaga gttgataccc aaggtgtccg tgtagaagca aaataaagaa
    19681 tagaagatag gatggggaaa ctcttaacca gagggtttca tatccttttt gtgaaaagaa
    19741 gtttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc tgctcaaaac
    19801 agtgttttga ggttgtggat agaactgacg aagtcagctc aagacactgt tttgaggttg
    19861 cagatagaac tgacgaagtc agtaacatat atacggtaag gtgacgctaa cgtggtttga
    19921 agagattttc gaagagtatt agttaaaaac gtgataaagg agaaataaag atggcagaaa
    19981 tttatctagc aggtggttgt ttttggggcc tagaggaata tttttcacgc atttctggag
    20041 tgctagaaac cagtgttggc tacgctaatg gtcaagtcga aacgaccaat taccagttga
    20101 tcaaggaaac agaccatgca gaaacggtcc aagtgattta cgatgagaag gaagtgtcac
    20161 tcagagagat tttactttat tatttccgag ttatcgatcc tctatctatc aatcaacaag
    20221 ggaatgaccg tggccgccaa tatcgaactg ggatttatta tcaggatgaa gcagatttgc
    20281 cagctatcta cacagtggtg caggagcagg aacgcatgct gggtcgaaag attgcagtag
    20341 aagtggagca attacgccac tacattctgg ctgaagacta ccaccaagac tatctcagga
    20401 agaatccttc aggttactgt catatcgatg tgaccgatgc tgataagcca ttgattgatg
    20461 cagcaaacta tgaaaagcct agtcaagagg tgttgaaggc cagtctatct gaagagtctt
    20521 atcgtgtcac acaagaagct gctacagagg ctccatttac caatgcctat gaccaaacct
    20581 ttgaagaggg gatttatgta gatattacga caggtgagcc actctttttt gccaaggata
    20641 agtttgcttc aggttgtggt tggccaagtt ttagccgtcc gatttccaaa gagttgattc
    20701 attattacaa ggatctgagc catggaatgg agcgaattga agttcgttct cgttcaggca
    20761 gtgctcactt gggtcatgtt ttcacagatg gaccgcggga gttaggcggc ctccgttact
    20821 gtatcaattc tgcttcttta cgctttgtgg ccaaggatga gatggaaaaa gcaggatatg
    20881 gctatctatt gccttactta aacaaataaa acagagagtg gggcttccca ctttcttcat
    20941 ttctagaata tgaatagaag ggatttatga aacacctatt atcttacttc aaaccctaca
    21001 tcaaggaatc aattttagcc cccttgttca agctgttaga agctgttttt gagctcttgg
    21061 ttcccatggt gattgctggg attgttgacc aatctttacc tcagggagat caaggtcatc
    21121 tctggatgca gattggcctg ctccttatct ttgcagtaat tggcgtttta gtggccttga
    21181 tagctcaatt ttactcagca aaggcagcag taggttttgc taaggaattg acaaacgatc
    21241 tttatcgtca tattctttcc ttgccaaagg acagcagaga ccgtctgaca acttctagtt
    21301 tggttactcg cttgacttcg gatacctacc agattcagac tggtatcaat caattcctgc
    21361 gtctcttttt acgagcgccc attatcgttt ttggtgccat ttttatggct tatcgaatct
    21421 cagctgagtt gactttctgg ttcttagtca tggttgccat tttgaccatt gtcattgtag
    21481 ggttatctcg attggtcaat cctctctaca gtagtctcag aaagaaaacg gaccaactgg
    21541 ttcaggaaac gcgccagcaa ttgcaaggga tgcgggttat tcgtgctttt ggtcaagaaa
    21601 aacgagagtt acagattttt caaaccctta accaagttta tgctagatta caagaaaaga
    21661 caggtttctg gtctagttta ttaacacctc tgacctatct gattgtcaat ggaactcttc
    21721 tcgttattat ctggcaaggc tatatttcaa ttcaaggagg agtgctcagt caaggtgctc
    21781 tcattgctct tatcaattac ctcttacaga ttttggtgga attggtcaag ctagccatgt
    21841 tgataaattc tctcaaccag tcctatatct cagccaagcg aatcgaggag gtctttgcgg
    21901 aatctcccga aaacatccat tcagaattag aacaaaagca agttaccagt ggtcgggttt
    21961 tacaagtcca agaattgact tttacctatc ctgatgcggc ccagccttct ctgagataca
    22021 tttcctttga tatgactcaa ggacaaattc taggtatcat cgggggaacg ggttctggta
    22081 agtcaagctt ggtgcaactc ttacttggac tttatccaat agataagggg agcattgacc
    22141 tttatcgaga tggatctagt cctcgtaatt tagagcagtg gcggtcttgg attgcttatg
    22201 ttccccaaaa ggtcgaactt tttaagggaa ctattcgttc caacttgact ttaggtttaa
    22261 atcaagaagt gactgatcaa gaactctggc aagccttgga aattgctcaa gctaaggatt
    22321 ttgtcagtga taaggaagga ctcttggatg ctctagttga agcaggaggg cgaaatttct
    22381 caggtggaca aaaacaaagg ctgtctatcg cacgagcagt cttgcgccag gctccgtttc
    22441 tcatcctaga tgatgcgacc tcagcactgg acaccattac cgagtccaag ctcttgaaag
    22501 ctattagaga aaattttcca aacatgagct taattttgat ctctcaacga acctcgactt
    22561 tacagatggc tgaccagatt ctcctcttgg aaaaaggtga gttgctagct gttggcaagc
    22621 acgatgactt gatgaaatcc agtcaagtct atcgtgaaat caatgcatcc caacatggaa
    22681 aggaggacta gaatgaaacg acaaactgta aaccagacgc tcaaacgttt agccgtagat
    22741 ttagcaaacc atcccttcct ccttttccta gcctttctag gaactattgc ccaagttggc
    22801 ttatcaattt atctacctat tctgattggg caggtcattg accaagtcct agtggctggt
    22861 tcatcaccag ttttttggca gatttttctc cagatgctct tggtggtaat aggaaatact
    22921 ctagtacaat gggccaatcc tctcctctat aatcgtctaa tcttctctta taccagagat
    22981 ttacgagagc gaatcatcca taaactccat cggttaccga ttgcttttgc ggatcggcaa
    23041 ggcaatggag agatggtcag tcgtgtaacc acggacatcg aacagttggc agctggcttg
    23101 accatgattt ttaaccaatt tttcattggt gttttgatga ttttggttag tattctagcc
    23161 atgctccaaa ttcatctcct catgactctc ttagtcttgc tgttgacgcc actgtccatg
    23221 gtgatttcac gctttattgc caagagatcc tatcatctct tccagaagca aacagagacg
    23281 aggggaattc agactcagtt gattgaagaa tcgcttagcc agcagactat aatccagtcc
    23341 ttcaatgctc aaacagaatt tatccaaaga ttgcgtgagg ctcatgacaa ctactcaggc
    23401 tattctcagt cagccatctt ttattcttca acggtcaatc cttcgactcg ctttgtaaat
    23461 gcactcattt atgccctttt agctggagta ggagcttatc gtatcatgat gggttcaacc
    23521 ttgaccgtcg gtcgtttagt gacttttttg aactatgttc agcaatacac caagcccttt
    23581 aatgatattt cttcagtgct agctgagttg caaagtgctc tggcttgcgt agagcgtatc
    23641 tatggagtct tagatagccc tgaagtggct gaaacaggta aggaagtctt gaccagtgac
    23701 caagttaagg gagctatttc ctttaaacat gtctcttttg gctaccatcc tgaaaaaatt
    23761 ttgattaagg acttgtctat cgatattcca gctggtagta agatagccat cgttggtccg
    23821 acaggtgctg gaaaatcaac tcttatcaat ctccttatgc gtttttatcc cattagctcg
    23881 ggagatatct tgctggatgg gcaatccatt tatgattata cacgagcatc attgagacag
    23941 cagtttggca tggtgcttca agaaacctgg ctcacacaag ggaccattca tgataatatt
    24001 gcctttggca atcctgaagc cagtcgagag caagtaattg ctgctgccaa agcagctaat
    24061 gcagactttt tcatccaaca gttgccacag ggatacgata ccaagttgga aaatgctgga
    24121 gaatctctct ctgtcggcca agctcagctc ttgaccatag cccgagtctt tctggctatt
    24181 ccaaagattc ttatcttaga cgaggcaact tcttccattg atacacggac agaagtgctg
    24241 gtacaggatg cctttgcaaa actcatgaag ggccgcacaa gtttcatcat tgctcaccgt
    24301 ttgtcaacca ttcaggatgc ggatttaatt cttgtcttag tagatggtga tattgttgaa
    24361 tatggtaacc atcaagaact catggataga aagggtaagt attaccaaat gcaaaaagct
    24421 gcggctttta gttctgaata agccattctc ttttgaaagt ttatggacga aaaaagttgc
    24481 cttcgagtga cttttttgtt acaatagcta gaaaaattgt ttactgtaat actcaatgaa
    24541 aatcaaagag caaactagga agctagccgt aggttgctca aaaaactgtt ttgaggttgc
    24601 agatagaact gacgaagtca gctcaaaaaa ctgttttgag gttgcagata gaactgacga
    24661 agtcagtaac cacacctacg gcaaagtgaa tttgaagtgg tttgaagaga gtacaacttg
    24721 tcttttagaa aaggagccta taatgaaagt ctttcagcat gtaaatatcg tgacttgtga
    24781 tcaagatttc catgtttatc ttgatggaat cttagcagtc aaggattctc aaatcgtcta
    24841 tgtcggtcaa gataagccag cgtttttaga gcaagctgag cagattatag actatcaggg
    24901 agcttggatt atgcctggtt tggtcaattg tcacacccat tctgcaatga caggcctgag
    24961 agggatccga gatgacagca atctccatga atggctcaat gactatatct ggccagcaga
    25021 atctgagttt actcccgaca tgactaccaa tgcggtcaaa gaagccctaa cagagatgct
    25081 ccagtcagga acaacaacct ttaacgatat gtataatccc aatggtgtgg atatccagca
    25141 aatttatcag gtggtgaaaa cttccaagat gcgttgttat ttttctccga ctctcttttc
    25201 ttcagagaca gaaacaactg ctgagactat aagcagaact cgatccatca tagacgaaat
    25261 cttaaaatat aaaaatccaa atttcaaggt tatggtagca cctcattctc cgtatagctg
    25321 cagtagagac ttgctggaag cgagtttgga aatggcaaaa gagctaaata ttccgctcca
    25381 tgtccatgtg gcggagacca aggaggagtc aggaattatc ctcaaacgat acggcaaacg
    25441 cccccttgct tttcttgaag aactgggtta tttagatcat ccgtccgtct ttgctcacgg
    25501 ggtcgaatta aacgagagag aaattgaacg cttggcatct tctcaagtgg ctatcgccca
    25561 caatcctatc agtaacctca aactggcatc aggaattgct ccaattatcc agctccaaaa
    25621 agcgggagta gcagtcggaa ttgcgactga ctcggttgct tccaataaca atctagatat
    25681 gtttgaggaa ggaaggactg cagctcttct tcagaagatg aaaagtgggg atgccagcca
    25741 gtttccaatc gaaacagctc tcaaggtact gacaatcgaa ggggctaagg tccttggaat
    25801 ggaaaatcag ataggaagtc tggaagtcgg caagcaagca gattttctgg tcattcaacc
    25861 acaagggaaa attcatctcc aacctcagga aaatatgctg tctcacctgg tttatgcagt
    25921 taaatctagt gatgtagatg atgtttatat cgccggagaa caggttgtta agcaaggtca
    25981 agtcctgaca gtagaacttt aaaagaaaaa ttacgaaaaa ttttaaaaaa agtttgcaaa
    26041 aatcttgcat tctttttttg actatgctat acttatatac ggtttaaaaa aactgcctaa
    26101 gacagtaggg gagctcgact cataaatatc ctaccgagga caaaacgtat catgtaaaaa
    26161 gaagcgtatt gtactttcgt gtctaggttt gggcgcgttt ttctttttga aaaattcccc
    26221 aagcaaaata attacggagg tgaacacact aatgagtgaa gcaattattg ctaaaaaagc
    26281 ggaactagtt gacgtagtag ctgaaaaaat gaaagctgct gcatctatcg tcgttgtaga
    26341 cgctcgtggt ttgacagttg agcaagatac agttcttcgt cgtgagcttc gtggaagcga
    26401 agttgagtat aaagttatta aaaactcaat cttgcgtcgt gcagctgaaa aagctggtct
    26461 tgaagatctt gcatctgtat ttgttggacc atctgcagta gcattttcta atgaagatgt
    26521 tatcgcacca gcgaaaatct tgaacgactt ttctaaaaac gctgaagcac ttgaaattaa
    26581 aggtggtgca atcgaaggcg ctgtcgcatc taaagaagag attcttgcac ttgcaactct
    26641 tccaaaccgc gaaggacttc tttctatgct cctttctgta cttcaagcgc cagtgcgcaa
    26701 cgttgctctt gcagtcaaag cggttgcaga aagcaaagaa gacgcggctt aatcttaagc
    26761 tacacagcgt agcctagcta cgaaaaaaac tattataaaa tttaaaactt atttggagga
    26821 aataacaatg gcattgaaca ttgaaaacat tattgctgaa attaaagaag cttcaatcct
    26881 tgaattgaac gaccttgtaa aagctatcga agaagaattt ggtgtaactg cagctgctcc
    26941 tgtagctgtt gctgcagctg atgcagctga tgctggtgct gctaaagatt cattcgacgt
    27001 tgaattgaca tctgcaggcg acaaaaaagt tggcgttatc aaagttgtac gtgaaatcac
    27061 tggtcttggt cttaaagaag ctaaagaact tgttgacggt gcaccagcac ttgttaaaga
    27121 aggcgttgca actgcagaag ctgaagaaat caaagctaaa ttggaagaag ctggagcttc
    27181 agttactctt aaataagagg catataccaa ttagaattcg gaatactata gtatcagcct
    27241 tttagaatcg tgaacacatt ttagaaactg ataaattaat ttagtttcct accaaaaacc
    27301 ctaccaaaaa ttaatttggc agggtttttc tttttaacaa atttatcgtg gagaacaatt
    27361 gaatattgtt ctcctttttt tattttcagg agggaaaatg acaaaagaat tacaatcatc
    27421 acgctatatt gtcatttcat ttttagtacg tgaaatggga attgacattg ttgaagccat
    27481 cggtcttatg gctgaattag aaaaaagtgg cttggttcga ttggaatcaa gtggagattt
    27541 aatactcaaa gaacttggag gagcgctatg aaacgaatta ccgcaaatca ataccaaact
    27601 tcagaacggt attataaatt acctaaaatt ctttttgagg atgagaaata tatggatatg
    27661 aaactagaag taaaggtggc ttattctatt ttaaaagatc gtttagaatt atctctaagt
    27721 cgtggttgga tagatgaaga aggagcggtc tatttagttt tttctaattc taagctgatg
    27781 aagttattag gttgttcgaa gtcaaaatta ctgtccatca aaaaaattct taaagaatat
    27841 gacttaattg atgaagtcca acagtcttcg agtgagaaag gaagactagc taataagatt
    27901 tatttagggg aattgtcttc taccccagta gctagttcga actgggctag tgttaaaaaa
    27961 agcctggggc aggatgaaaa tgaaacggcc cccgtctcac attcagcccc tagtgatact
    28021 gaagttagtg agactaaata tagtgagact gattctttat ttattgagga tgaggaggag
    28081 aggaatactc aacctatctt gaaaagaaaa gtagaaaagg tcacaaaata tgatcgagat
    28141 tatatttggg gattggtaca agatcaattt agacgagagg gtttttctga aacagctagt
    28201 gaaattgcta tgactgattt tgagagaatc tatcagtatg ctcttgacaa tgttcgcttt
    28261 gttagacgtg cggaagtact tgctgaattt gtgtttaacg gcttgtattc tgtttggaat
    28321 aaccgtgtta gaaaaggagg tggttaaaat gtcgccaatc gagtgggtat tagtcattct
    28381 tattttaatt tcaagtggtg tgacactttg gacattgata gtcgattaga aaggggtaat
    28441 caagaaatga gatttattga tttattttca ggtataggtg gttttcgact tggaatggaa
    28501 agtgttggac acgagtgtat tggattttgt gagattgata aatttgctag agaatcttat
    28561 aagtccattt ttcaaacgga aggagaaata gaatttcatg acatacgaga tgtttcagac
    28621 gacgaattta aaaaacttag agggaaagtc gatgtcatct gtgggggatt cccttgtcaa
    28681 gcattttcaa tcgcaggaag acgattggga tttgaagata ctagaggaac tttgttcttt
    28741 gaaattgctc gggcggccaa acaaatccaa ccacgttttc tttttcttga aaatgttaaa
    28801 ggcctactca atcacgataa gggacggacg ttcaccacaa tccttaccac acttgatgag
    28861 ttggggtttg atgttgagtg gcaggtgctt aacagtaagg attttggcgt tccccaaaac
    28921 agagagaggg tgtttattat cggacattct agaaagaaag gtaccagact cctatttcct
    28981 ttcagacgag aaggtcaagc aactaaccct gagactttga aagcactagg gaatttaaat
    29041 ccatcaagaa gtggaatgag tggtaaagtc tattattcag aaggtcttgc gccaacctta
    29101 gttcgtggaa aagtagaagg atttaaagtt gcgattcctt gtatgacacc agacagatta
    29161 gaaaaaagac aaaatggtag acgtttcaag gataatcaag agccaatgtt tactttaaat
    29221 actcaggatc gccatggtat tgtcgttgtt ggagatttac cgactagctt taaggagaca
    29281 ggtcgtgtct atggaagtga ggggctatct ccaacactta ctacgatgca aggtggagat
    29341 aaaatcccca aaatactgat tccagaacca atccaatttc taaaagtccg agaggcaacg
    29401 aaaaaaggat atgctcaagc agaaatagga gattcaatca atttggaaag accaggttct
    29461 cagtatcgtc gtggtagagt tggaaaaggt atagcgaata cgttaacaac tagtggtcaa
    29521 atgggagtag tagtggctag ctatgaagga gaagataagc aagtttacca tgttgctggt
    29581 attttaataa atggacaatt ttaccgtctg aggataagac gaatcactcc taaagagtgt
    29641 tttcgcttgc agggatttcc tgattgggct tttgaagctg ctagaaaagt ctctagtaat
    29701 agtcagctct ataaacaagc tggtaatagt gtaaccgttc ctgtgattgc cgcaatcgca
    29761 aagaaattaa aagaaataga ggaaaaagat gaaagcgtta aataaagagt caatactata
    29821 ttgtgatgaa ttagaaacag aattacatga tgcagaaatg atgcagttgg atgaacaaat
    29881 atttttgatg cccaattatc catgtgagtt tgaagtaaaa tttttagatt attaccataa
    29941 aaaacataat tacccactat tttatgaatc ctatcttcaa aacattatgg aattccttga
    30001 aagtcaggat ataaagaacg gagctgatgc ttttgtagat gataatcaga atcttgtttt
    30061 tgttttatat gggcaaggct atcgagccga gggaaaagag ggaatactta caacccaagt
    30121 aactgtaaaa gcttatgatg aagacaagaa accgattaac ttctccaatt tattagattc
    30181 cttaatcgtc tcagaatatc aaatggaacc gaatctttgg gaggtctccc atgattgatc
    30241 tctatctaag taaaaataac catagaaatc aacttctttt agacttcttt caaaactatg
    30301 gcatcgaggt atcttgtcat tcagtttctg aaatgacaaa ggagaaatta attgagatga
    30361 tgagctattc ttcagattgt ttcgagtttt tatctccaaa tttattacgt tttaagaatc
    30421 gagataacct aaaattaaca gatttcatcg aaatgatatt aaaaaatcct gaactaacca
    30481 tcagacttcc tcttgcggtt tcaaataagc gagtttatcc aaatttgaat ctggaggagg
    30541 ctagagcttt attgccaaga gatacgaaac aattgattta catggcacaa acacattatt
    30601 tatctaacta aaggagaatc aatatggcag aacgattttg ggagaacttg tccattattt
    30661 tggctgaaag aaatatcagc tggattgagt taaccagaaa aatgtttgcg ggagagtttc
    30721 actatccaag tgaattgaat cgtttgtatc aaaagattag gcactacaag gtggaacaac
    30781 gaatgcctca aggtccatgg gtagaaagga ttgtacaggt attagattta gattatgaag
    30841 atttatttcg gaggtaacta tgaaaagaat aattccagtt tatatattcc aacaagtaaa
    30901 tgtcctattg gtatctctat acttactgaa atttctttgt atcggtgagt taactatact
    30961 acagattctc tatggttcgt cactcatttc ttttttatgg atgtatggcc aacgtaaaaa
    31021 agtgcataag gtcagtatga aatctaggat gaaatggctt ggtattggat tagctagcct
    31081 actgattata agtctatgtt ttagcctaat ccatgctcaa ggaagcacga atcaagcaaa
    31141 cttaattggc ctacaacatc aggttccttg gttttcattt ttattgttct taatcaatgc
    31201 gagtatggtt gaagaatttc tgtatcgaga aatcttatgg aacttggtca gaaaattaga
    31261 catcagaatt gccttgacaa gtgttttatt tgccttagta catcatccag gaaccattct
    31321 agcttggtgt ttgtatgttt cacttgggat gtttttaggg atggtgcgct ataaatctga
    31381 tttatgggga agtatgggtt tacatttggt ttggaatcta ctagtttata gtttgctgct
    31441 tttttaaaat aggtaacctg atttttaggt aacttctaaa actaacttcc agttttggga
    31501 gagagatgga agttactttg aaaagttacg gaaacattat tttctatgtg acaacattgt
    31561 atcattgctt gcattttact gctggagaga gtataatgaa ggtggataaa gcacttaatc
    31621 ccaaaaattt aaataggagg agttagctat ggcagaagaa gtattaaatc tttaattagt
    31681 atctgttcaa gttgacgaaa cagatgaagt tgatggaatg cgatttagta cattcagtac
    31741 aaac
//
LOCUS       DAVMEF010000004        28243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMEF010000004 DAVMEF010000000
VERSION     DAVMEF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.004"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            55..1341
                     /locus_tag="VYQ30_000105"
     CDS             55..1341
                     /locus_tag="VYQ30_000105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1965279.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(1584..2732)
                     /locus_tag="VYQ30_000106"
     CDS             complement(1584..2732)
                     /locus_tag="VYQ30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1965280.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKTKFGSIQLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATELHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(3103..3510)
                     /locus_tag="VYQ30_000107"
     CDS             complement(3103..3510)
                     /locus_tag="VYQ30_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEV1965281.1"
                     /translation="MALFGKKQDESLEVELFTEEPNERVFEFKKSKTVVRIDDYFIRI
                     ARKSNVSNVLLHGLDGEKSILLSEITAYQLKEPGSTVGYLQLVYPGSSDTKGGVFDAV
                     KDENTVTFTKDEKASILELKKAIEKALKDKVKK"
     gene            complement(3555..3938)
                     /locus_tag="VYQ30_000108"
     CDS             complement(3555..3938)
                     /locus_tag="VYQ30_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1965282.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPKEFDYLK
                     FMSYYNLKTVTNEVMVKEEYQTLIG"
     gene            complement(3951..4316)
                     /locus_tag="VYQ30_000109"
     CDS             complement(3951..4316)
                     /locus_tag="VYQ30_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1965283.1"
                     /translation="MFLAFDRIKELADKQKISLNILEEKLGYSTNYLYSLKKGNPKSD
                     RLQEIADYFGVSTDYLLGRTENPNLADDTKEYIWQGKVLNVEEMASNVMMFGGRELTD
                     EKKKIIQSIIEGYLKEAGD"
     gene            4603..4749
                     /locus_tag="VYQ30_000110"
     CDS             4603..4749
                     /locus_tag="VYQ30_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965284.1"
                     /translation="MNEEDLKELLELLATDYGRGYLDGVVGGLSMLLKISKEAEKNRK
                     EEYE"
     gene            4793..5167
                     /locus_tag="VYQ30_000111"
     CDS             4793..5167
                     /locus_tag="VYQ30_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965285.1"
                     /translation="MIETLEIKINKKTYFISARADVPVRYKVIFGRNIFDDFSKIKNN
                     KDDTLSLCLLFCMTDKSSEEFCKFFIENNSYITNKHHYEAIRLFEKHFISKRVTYSLE
                     DIVQILTIYLENTKTQIGGKNE"
     gene            5160..5363
                     /locus_tag="VYQ30_000112"
     CDS             5160..5363
                     /locus_tag="VYQ30_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1965286.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYG
                     KGSDYVKEKVVDILGIENPWRNH"
     gene            5506..6222
                     /locus_tag="VYQ30_000113"
     CDS             5506..6222
                     /locus_tag="VYQ30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV1965287.1"
                     /translation="MNEIFNFQGQEVRTLTIDDKPWFVGKDVADILGYANSRKAIFDH
                     VDDEDKTDGVTIRDTMGRNQNPIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIR
                     KQGGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAKSLLKKRK
                     ARVVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMT
                     WEPSTNTKMKIMELNSFDEG"
     gene            6235..6492
                     /locus_tag="VYQ30_000114"
     CDS             6235..6492
                     /locus_tag="VYQ30_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1965288.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            6578..6898
                     /locus_tag="VYQ30_000115"
     CDS             6578..6898
                     /locus_tag="VYQ30_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965289.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIRGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPIGLRPEPLALPEPKQSA
                     IRFL"
     gene            6914..7210
                     /locus_tag="VYQ30_000116"
     CDS             6914..7210
                     /locus_tag="VYQ30_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965290.1"
                     /translation="MEELIEWLDNLIMIVKELEGRESTSRHFITIWENDYKNLLLVKE
                     YLTDYEKLAKDYRYVTLKNKLLKIEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            7203..8012
                     /locus_tag="VYQ30_000117"
     CDS             7203..8012
                     /locus_tag="VYQ30_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1965291.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNKNRLDK
                     DKNKKRITTTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENNVDLVKEALKTA
                     INSGKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            8152..8922
                     /locus_tag="VYQ30_000118"
     CDS             8152..8922
                     /locus_tag="VYQ30_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1965292.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQARVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            8937..9131
                     /locus_tag="VYQ30_000119"
     CDS             8937..9131
                     /locus_tag="VYQ30_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965293.1"
                     /translation="MFILRHGTREDKPFLRSVVIGVTGLDISCSEEKKAMRFVSRGAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            9485..9649
                     /locus_tag="VYQ30_000120"
     CDS             9485..9649
                     /locus_tag="VYQ30_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965294.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEL"
     gene            9639..9848
                     /locus_tag="VYQ30_000121"
     CDS             9639..9848
                     /locus_tag="VYQ30_000121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965295.1"
                     /translation="MNYDKRTVIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRRKAEELEDDGRVKEKS"
     gene            9820..10137
                     /locus_tag="VYQ30_000122"
     CDS             9820..10137
                     /locus_tag="VYQ30_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965296.1"
                     /translation="MMEELKKKVNAVYNWTVEDGKPQPPQQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            10139..10813
                     /locus_tag="VYQ30_000123"
     CDS             10139..10813
                     /locus_tag="VYQ30_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1965297.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLAESEIGH
                     ADEAPRYVKNILARLRELPLHDREVWLKAIMSEFEQDFSHAKWREGYEQGKIEGMVER
                     EKVIVPRFVAEWIEEARKACKDVVELFEFDFTNDEVRKWFMQERPFDLVARAWLDGYE
                     VEEEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDANFGWVFDCEGI
                     EIEEVE"
     gene            10813..11100
                     /locus_tag="VYQ30_000124"
     CDS             10813..11100
                     /locus_tag="VYQ30_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965298.1"
                     /translation="MTQTLEEGMKNQSKCIKIPMEIRPFDVGYRIVNKHGQALALKNG
                     ASIFALPSLAEKAIKKEFGKNDPDFDIEKHFVEEVAIVNLSKFHSYFEGVE"
     gene            11100..11288
                     /locus_tag="VYQ30_000125"
     CDS             11100..11288
                     /locus_tag="VYQ30_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEV1965299.1"
                     /translation="MSCSENLKKEKELTAAISNFKIEVLQNDDKLSSLSLSNIKRQAR
                     DLYECLVWLQYNAEESGR"
     gene            11285..11623
                     /locus_tag="VYQ30_000126"
     CDS             11285..11623
                     /locus_tag="VYQ30_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965300.1"
                     /translation="MSYDLEILVKIESGDYICIAEPKYSSPTYNLGRMFRVAMNWDFD
                     QDTTYNIADVLDNIQRGISELERYPEKYVQYEPENRWGTVSVALEVLKSLKECILEQD
                     IDTKYLYMRW"
     gene            11625..11885
                     /locus_tag="VYQ30_000127"
     CDS             11625..11885
                     /locus_tag="VYQ30_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1965301.1"
                     /translation="MRRFIAIWILVSAGLNIWHMDRIRDLEEKKPMVVYKADNAGAEI
                     FGRVVEKGRHGKLYTVTIRDYGIFVVTKEQFEKIRVGDEVLL"
     gene            11885..12343
                     /locus_tag="VYQ30_000128"
     CDS             11885..12343
                     /locus_tag="VYQ30_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965302.1"
                     /translation="MDDILQALAKMLNMTVDEVSSLLTTFKGNAPQIYEMFVKEKMFY
                     DLFSLFQIMSIVIFSISAVVLAVLTLIYFTYDGGFVYSYDIRTGKTEEEIKLERIERK
                     RKDLKIPLKISCISSSASLITLVIAIVLKATLAPNYIFIVNEILPKLTKR"
     gene            12457..12735
                     /locus_tag="VYQ30_000129"
     CDS             12457..12735
                     /locus_tag="VYQ30_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965303.1"
                     /translation="MKRLGIIIGAVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQAL
                     GMDALLSFIWPVLSSKKESEEDYSYAVKSSISKIITCAFLIWLASLFI"
     gene            12747..13148
                     /locus_tag="VYQ30_000130"
     CDS             12747..13148
                     /locus_tag="VYQ30_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965304.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMP
                     SIYLKGLLSGYAWTEDEEIIGNVYENLVYIF"
     gene            13166..13567
                     /locus_tag="VYQ30_000131"
     CDS             13166..13567
                     /locus_tag="VYQ30_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1965305.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSSP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            13758..14300
                     /locus_tag="VYQ30_000132"
     CDS             13758..14300
                     /locus_tag="VYQ30_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1965306.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            14677..14997
                     /locus_tag="VYQ30_000133"
     CDS             14677..14997
                     /locus_tag="VYQ30_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1965307.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            15134..15526
                     /locus_tag="VYQ30_000134"
     CDS             15134..15526
                     /locus_tag="VYQ30_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEV1965308.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            15519..17249
                     /locus_tag="VYQ30_000135"
     CDS             15519..17249
                     /locus_tag="VYQ30_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV1965309.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            17257..17475
                     /locus_tag="VYQ30_000136"
     CDS             17257..17475
                     /locus_tag="VYQ30_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965310.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            17493..18695
                     /locus_tag="VYQ30_000137"
     CDS             17493..18695
                     /locus_tag="VYQ30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1965311.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            18679..19254
                     /locus_tag="VYQ30_000138"
     CDS             18679..19254
                     /locus_tag="VYQ30_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV1965312.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            19251..20417
                     /locus_tag="VYQ30_000139"
     CDS             19251..20417
                     /locus_tag="VYQ30_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1965313.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVLAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTPGKMEERGGK
                     TLGQREAFNHYLRTKEARADGFKSAEGEAIIPVELMTPKEAKQDKTDLTSLVNIVNVK
                     NASGKWSVVKLTDQEMNTVEELEENPELAKPTFTKVNYEIQTRRGHLPVSQEFIDDAD
                     YDVMGLVAKQTKNQERITKNKEIAKVLKTATSKSAAGLDGLKDILNVELKTYYNATIV
                     CTQSMFAALDKIKDKDGRYMLQTDITSPTGYKFAGRVIVVYPDDIIGESKGDLKAFIG
                     DVGEFATLFDRAQTTVKWQDDKIYGQYLATANRFDVVKVDGDAGFYVTYTDAVL"
     gene            20429..20698
                     /locus_tag="VYQ30_000140"
     CDS             20429..20698
                     /locus_tag="VYQ30_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965314.1"
                     /translation="MVYKVIRPFKDLRDPKKHCYKVGDNYPRVKHKLDKEFAETLLNG
                     ANSAGSIFLMNVEDKDMSKDDTLTTEVEKEETKVVDGKPEEGDEE"
     gene            20701..20982
                     /locus_tag="VYQ30_000141"
     CDS             20701..20982
                     /locus_tag="VYQ30_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965315.1"
                     /translation="MDNVQLLELLKLKLGIATKLRDKPLEKIIEAVKTELEDNLGVLL
                     DLDSSEDQMFVVDFAAFRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            20969..21268
                     /locus_tag="VYQ30_000142"
     CDS             20969..21268
                     /locus_tag="VYQ30_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEV1965316.1"
                     /translation="MWNHEITLISKEVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEMLK"
     gene            21265..21624
                     /locus_tag="VYQ30_000143"
     CDS             21265..21624
                     /locus_tag="VYQ30_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1965317.1"
                     /translation="MSLTSDLANEIAKAMAEYSAEVEDKIDLIAEDVVNEAVTELKAT
                     SPKRHGKYARNWRFKKNAKGSYVIYNAAPTYRLTHLLENGHVLRNGGRSRAFPHIKPV
                     EEKVKENFEKRIKEIGK"
     gene            21621..21944
                     /locus_tag="VYQ30_000144"
     CDS             21621..21944
                     /locus_tag="VYQ30_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965318.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKTGNAPDLPYLVYYESIPAINA
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWTTHELFFDVQEETFIEIERLYVKSY
                     TVYLY"
     gene            21956..22534
                     /locus_tag="VYQ30_000145"
     CDS             21956..22534
                     /locus_tag="VYQ30_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1965319.1"
                     /translation="MNQENKVTFGLKNVHVAPIKSIGADGVIAYDEIFRFPGAMELTL
                     DPRGESIPIKADDIDYHFMNSNEGYEGKFKISHIIEMFATKILGEIKDAQTGVLTEKA
                     DAEFTSFALMFEFSGDKNKTRHVLYYCSASRPGNGSKTKNGTNVNERELGFKASPRPL
                     DSVVKRSITSADNKEIYDNWFKKVYEPTAVAA"
     gene            22546..22965
                     /locus_tag="VYQ30_000146"
     CDS             22546..22965
                     /locus_tag="VYQ30_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965320.1"
                     /translation="MRKIVWVGVQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATDIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            23242..25992
                     /locus_tag="VYQ30_000147"
     CDS             23242..25992
                     /locus_tag="VYQ30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1965321.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMVKVTSSFK
                     LQESALGSNASEAERNALAQKKIGVQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANELYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFIVILGVA
                     LTIVGALAPIFLTLQAVFMSSFGAMIAAALPIIGIISGVVVAIAAIVAIVKYLWETNE
                     GFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVAWWTENQELIRASAETVWNAIY
                     TVISTILEILGPLIQTGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIINGDW
                     SGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNVLNGISGTVSNIWNGIKDTVSNV
                     LNGISGTVSSVWEGIKSTISGAINGARDAVSSAIEAIKGLFNFSISWPHIPLPHFYVS
                     GSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLNDKT
                     LGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRKTQLRGG
                     FT"
     gene            25989..26711
                     /locus_tag="VYQ30_000148"
     CDS             25989..26711
                     /locus_tag="VYQ30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1965322.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSITLGESKTALLEHGGI
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKYFKGSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSAVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            26712..>28243
                     /locus_tag="VYQ30_000149"
     CDS             26712..>28243
                     /locus_tag="VYQ30_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1965323.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAITVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQAKFSNEGIDKISDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDVFKKAIAYEFDALKEEYISLILDDKAGAGG
                     SRTSGGLSSAADAILGVTESAQEVALEKALQNADLDFDHKAGLLRQEISDGIELAKAK
                     AEEVKQELSDTINQRFNSFDNGPLKEAKRRAEEALRNAGASSLLAQEAKRIGLDSVAR
                     LEEFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELAGA
                     STL"
BASE COUNT         9117 a         4658 c         6456 g         8012 t
ORIGIN      
        1 gagagtcctg ccatgtatgt gagaaaggaa gagcctgagg gctcagacaa aattatgact
       61 tcagttgttg ttgtaggtac ccaatggggt gatgaaggta aagggaagat tacagacttc
      121 ctttcagcga atgcagaagt gattgcacgt taccaaggtg gtgataatgc tggtcacacg
      181 attgtgattg acggtaagaa atttaagttg cacttgattc catctgggat tttcttccct
      241 gaaaaaatat ctgtcattgg gaatggtatg gttgtaaatc ctaaatctct tgtaaaagag
      301 ttgagctatc ttcatgagga aggtgtaaca actgataact tgcgtatttc tgatcgtgcg
      361 catgttattt tgccttatca tatcgagttg gatcgcttgc aagaagaagc taagggcgac
      421 aataagattg gtacgacaat taagggaatt ggtccagctt atatggacaa ggctgctcgt
      481 gttggaattc gtattgcaga tcttttagat aaagatattt tccgtgagcg tttagaacgt
      541 aaccttgctg aaaagaatcg tctttttgaa aaattgtatg acagtaaagc gattgttttc
      601 gatgatattt ttgaagaata ttacgaatat ggtcaacaaa tcaagaaata cgtgatagat
      661 acatctgtta tcttgaatga tgcgcttgat aatggcaaac gtgtgctttt tgaaggtgca
      721 caaggtgtta tgctagatat cgaccaaggt acttatccat ttgttacgtc atcaaaccct
      781 gtagctggtg gtgtgacaat tggttctggt gtcggtccaa gcaagattga caaggttgta
      841 ggtgtatgta aagcttatac gagtcgtgta ggagatggtc ctttcccaac tgagttgttt
      901 gatgaagtgg gagaacgtat ccgtgaagtg ggtcatgagt atggtacaac aactggtcgt
      961 ccacgtcgtg taggttggtt tgactcagtt gtgatgcgtc atagccgtcg tgtttctggt
     1021 attactaacc tttctttgaa ctctattgat gttttgagcg gtttggatac tgtgaaaatc
     1081 tgtgtggcct atgatcttga cggtcaacgt attgactact atccagctag tcttgagcaa
     1141 ttgaaacgtt gcaagcctat ctatgaagag ttgccaggtt ggtcagaaga tattaccgga
     1201 gttcgcaatt tggaagatct tcctgagaat gcgcgtaact atgttcgtcg tgtgagtgaa
     1261 ttggttggcg ttcgtatttc tactttctca gtaggtcctg gtcgtgaaca aacaaatatt
     1321 ttagaaagtg tttggtccta agagattttt aagatttgtt taagataggt cgggtatact
     1381 atagacagtt acaagaagac ctcctaactt gttgtaacaa atatcctaaa cttttctttt
     1441 tcataataat ctcccttaac tccaccgaat caggtggagt tttttagctc tatttcaggc
     1501 ttttggggac tattctaaaa ataatttttc gatatttttc ggtatttttc ggtttttggt
     1561 cggggaattg gcggggactt tttttagcga atatgactaa gaaataggtc tgtggtcgct
     1621 tcagcaagtt cgtcctctac ttgattgtaa cgatccgtca tataaacctt tgtatgcccc
     1681 agcgcctggc ttaattgttc aagcggaacc cctgcaataa tgctttgagt cgtgaagaag
     1741 tggcgcatca tgtgaggtgt tacatgcaat tctgttgctt cattcactag attgaagttt
     1801 ctatttagct gatttggatt gatgagacca cctttctcgt tgatagttat ataatccttg
     1861 tgctgttcct tgataattcc taactttcgc ttaatcttag aagcttcagc tatcagataa
     1921 tagatcaggt ctgttccgat atcatcaagg cagacatatc gctctgaatc cttcgtttta
     1981 agccctcctt tcccttttaa ggtctggttg cttcgactgt ctctaagatg cagtatagcc
     2041 cgtccgctgt cgttctgagt gatgtccatt ggacgcaatc caaagacttc tcctcttctc
     2101 aatccaaaaa ttgtcagata ggtcagagcg tagaattgtt ttggcataat ctcttctgcc
     2161 tttgctatcc aagtcttgaa ctctttgaga gtcactttct tgtttgcagc agggatatca
     2221 ctctggccga tgaaaacacc tttcaagcga tttgagagca gatttccatt tttgacggca
     2281 tcattcagca atgccatgaa gctggaattg agggtttgaa cagtgtatct ggtatggttc
     2341 tgcaactttt cagcgataaa gagttcatac tcatttctat ccaaattttt aagctgaata
     2401 gaaccaaatt tggttttgat atgattctta tagaggttat cattgaggta gtaggaagtg
     2461 tcattccagc gccctgttga caatctcttt tcagaataaa tatcccaata ttgatcaagt
     2521 gtcagattcg tattgatacc taattcttgt tcttggattt gttgctcaag ctctaccaag
     2581 gctgcacgag cttgagggag agttgtgaaa ccacttttac ttttttctct ttttttacct
     2641 cggaagaaaa aagaacgtct gacatagtaa cgcttgcctt tagcagtctc atagtaatag
     2701 atatttgggt attttgtttt attatatttc attgtattct ccttgtttat cggcttctgg
     2761 acaaggtcta aacattgaga atattgacat cacccctttc atggtgtaaa atagggtata
     2821 gaaaagaggc ctttttaatg gctgattttt tataagggtg ggcttcacaa tcaaactttg
     2881 gcgagggaga ttgtggagct tttttgtttt taattcaaca aaaaacggta actaaattta
     2941 tagttaccgt ttctgcgtgg cagcttgtgc caaccaggtt atttgcacta ggatttcccc
     3001 taggttagta actatatatt atcaaatata tttcattttg tcaaaaatgg aaagttgagt
     3061 cttcacactc agaatttaga gatagagagt gtggggattt ttttatttct tgactttatc
     3121 ttttaaagcc ttctctatgg ctttctttaa ttccaaaata gatgcttttt catctttggt
     3181 aaaggttact gtgttttcat cttttacggc atcaaacaca ccaccttttg tatcagaaga
     3241 accagggtaa acaagttgaa gatagccaac agttgagcca ggttctttca attggtatgc
     3301 tgtaatctca gagaggagaa ttgacttttc gccatcaaga ccatgaagaa gaacattaga
     3361 tacatttgac tttcttgcaa tcctgataaa ataatcatcg attcttacaa cagtttttga
     3421 tttcttaaac tcaaaaactc gctcattcgg ttcctctgtg aagagttcaa cctctaaact
     3481 ttcatcttgc tttttaccaa acaatgccat aagtagttcc tttctttttc tgcttcagca
     3541 gtttataaac atatttaacc aattaaagtc tgatattcct cttttaccat gacttcattt
     3601 gtcacggttt taagattgta gtaggacatg aatttgaggt aatcaaactc tttggggtcg
     3661 tctaagcttt ctagtgcgtc ttttacgaga tgatggatca tattcctatc agcttcgttt
     3721 tcacagcgta agcgagcgtt ctggtattct gatcgtgtgt gatccttgtg tcctagttca
     3781 tgaagtagga ccttaactct ctcttttttg ctgagtttat tagacaagaa agctgtattg
     3841 gtttcttttt cgtaaaatcc aagttcatca ggtattagct cgccgtcaaa atcgacaatg
     3901 cgaacttgaa aatgacttat aatttctttt tcggtcacta agcagtacct ctaatcacca
     3961 gcttctttga gataaccttc aatgatagac tggatgattt tcttcttttc atctgttaat
     4021 tctcggccac caaacatcat gacattagat gccatttctt caacattgag aactttgccc
     4081 tgccatatgt attcttttgt atcatcggca agattaggat tttcagtacg gcctagtaag
     4141 tagtctgtac taacaccaaa ataatctgct atttcttgta atctatcaga ttttgggtta
     4201 ccttttttca gactataaag ataatttgtg ctgtatccta atttttcttc taaaatgttt
     4261 aaagaaattt tctgtttatc agccaattct ttgattctat cgaatgctaa gaacattgtt
     4321 ttatcaacct ttctaagcac tacgaaaaaa atattttaaa attaattata aaaactgttg
     4381 acaaattcta aaactagttt taaaatagta ttcgtaagct aaagagttag cgaactagac
     4441 aactaaaaaa taaagcctta caaaactgat tggcgtccgt tttctatagg taaaacttac
     4501 tttagtaggt cttttctcta tgtctttatt ctaaaactag ttttagaatt tgtcaagtag
     4561 ttcgctaact ttttagataa tttttttaaa aggaggtcag ggatgaacga agaagacctg
     4621 aaagaattat tggaactctt agcgacagat tatgggcgag ggtatctaga tggagtagtt
     4681 gggggacttt caatgctttt gaaaatttca aaagaagcag agaagaatag aaaggaagaa
     4741 tatgagtaaa gcaaaattat tagtcacata aagtaggtaa acgaggtagg aaatgataga
     4801 aacactggaa ataaaaataa acaaaaagac atacttcata tctgcaaggg cagatgttcc
     4861 agtcaggtat aaagttatat ttggcagaaa tatcttcgat gacttttcga aaataaaaaa
     4921 caataaagat gacactctat cactttgtct attattttgt atgacagaca aatcgtcaga
     4981 agaattctgt aaatttttta ttgaaaataa ttcatatata acaaataaac atcactatga
     5041 ggcaataaga ttgtttgaaa aacattttat ctctaagcgc gtaacatatt cattagagga
     5101 tatagttcaa atcctaacaa tatatctaga aaatactaaa acacagattg gaggaaaaaa
     5161 tgagtaaaga actaaaaata attaaggcta aaattaaaac tcgtttgatt gagttggata
     5221 tgactcaagc cgaattggca aaacaagtat ctgtagcatc atcagttatt tcagagctac
     5281 tgaaatatgg aaaaggaagt gattatgtga aagaaaaagt cgtagatatt ttgggtattg
     5341 aaaacccttg gagaaatcac tgagaggtcc gtacatgcaa gcgaaaataa tactgaattg
     5401 gcagaagaaa aatcaccaac ttagtcagat gatgatcgat agtcttgagg gactagatgt
     5461 ttgggaaact attttaacac taggaaaagt aagaagagga acattatgaa cgaaattttt
     5521 aattttcaag gacaggaagt ccgtactttg acaattgatg acaagccttg gtttgtcggt
     5581 aaggatgttg cggatatctt aggatatgcg aattcaagaa aagcaatttt tgaccatgta
     5641 gatgatgaag ataagacaga tggggtaacg attcgtgaca ccatgggtag aaatcaaaat
     5701 cctatcatca ttaatgaatc tggtctctac tccctcatct tatccagcaa gttgcctcaa
     5761 gcaaaagagt tcaagcgttg ggtgacatca gaggtcttgc cagctattag aaaacaaggc
     5821 ggatttatcc gagaggactt ggatgaagat gctttcatcg ctctgtttac tggacagaag
     5881 aagttgcgtg agcaacaagc gactatgctg gaagatattg actacctcaa gagtgagcaa
     5941 ccgattcacc caagctatgc taaatcgctc ctgaagaagc gtaaggctcg ggtcgtggct
     6001 tgcttaggtg gcattgatag tccggcttat gcggataaga ctttcgctca gtcagtcttt
     6061 agacaagctg agattgactt taaagaccac ttcaacatta atcgctatga cttgctaccg
     6121 aaaaaattcg cagatgcagc cttggcctat tggatgactt gggagccaag caccaatacc
     6181 aagatgaaaa tcatggaatt gaactcattt gacgaagggt aggaggggaa gaagatggac
     6241 aatgttctac tttcactgtc tgaatggatt aaatccatta tcaaggacac aatcacaagg
     6301 ctagtcgaaa tagaaaaaga tagtgaccac tatccagagc tgatggatgt gaacactacc
     6361 tgtgattttc taggaattaa gtatgccaca ttttcagata attatcgtta cttaaaggga
     6421 tttccaaagg aattacctgg taagaaatgg tcaaaaagag ccatcaaaga atggctctct
     6481 aatcaaatat aataacttta ctaaaaggct tctggacaag gtcttagcaa aattatttga
     6541 ctatattata gcacaaaaag aggataagga gataaaaatg tttgaaccac cgattttaga
     6601 ccagctgatg ggggttggag ccttactgct tggatttgca ggggcttgcc gtcatatcaa
     6661 attgcaggaa caacgcaagg aagaagaaag acgagaagag caagaatttg cgtctatgat
     6721 tattcgaggc tataaccatg cttacgaacg tggtagagag gctgaacgtc aagaaatccg
     6781 caagaatatt cgtcgtccgt tcaagggctt tacctacgac aatgaaccgc ctataggctt
     6841 gcgtcctgag ccgttagctt tgccagaacc taaacagtct gcaatcagat ttttgtaatg
     6901 aggaggtcag gaaatggaag aattgattga atggctggat aacctgatta tgattgttaa
     6961 agaactggaa ggaagggaat caacttcaag acattttatt acgatatggg aaaacgatta
     7021 taaaaatcta ttactagtca aagaatacct aaccgactat gaaaaactag caaaggacta
     7081 tcgttatgtg acccttaaaa ataagctgct aaagattgaa aaaatggagc tggaaggcag
     7141 gtacatctat gaagatatgc ggatgaagta tcgcgctaac cgtaggaagt ggggtgctag
     7201 gtatgtctga aattaagtgg ataaaaatca caaccgatat ttttgacgat gaaaagattt
     7261 gcctgattga tgccttgcct gatcctgatg ccatcttagt gatatggttc aagattttga
     7321 cacttgctgg aaaacataac agtaatggtt tgttgatgat gactgataag gttcactata
     7381 cagatgaaat gttagctact atttttcgta gaccattgaa tacagtaaga atggctattg
     7441 gagtttttga acagtttggg atgattgaga ttatcgatgg tattattagc ttgccaaatt
     7501 gggaaaaaca tcaaaacgtt gacggaatgg agaaaatcaa ggaacagaca cgtaaccgtg
     7561 tagccaaata ccgtaaaaaa cagaaaaatc ttgctcttgg taacgttaca ggtaacgtta
     7621 cagtaacgga cggtaacgca ctagaagaag ataaagataa gaataagaat agattagata
     7681 aagataagaa taagaaaaga ataactacta ctactagtag tggtagtgaa gaaaatatct
     7741 tagaactttt tcaatctgag tttcgtagac tcttatctgg atttgaaatt gaagaaatca
     7801 accatctact aaatgagaat aatgtggatt tggtgaaaga agcattgaag actgctatta
     7861 actcaggaaa gccgaacatc aaatatattg gtgggatttt aagaaattgg cagatgaaca
     7921 atgttaccac tgttgaacag gttcgtcaat cggaaaagaa gaacaaggat aagaaagaag
     7981 aacaggaggc caaggacgaa tgggggtact agaactaatt gaacaattcg agattgacta
     8041 ttatccgtta agctacgaga agaaaactct tttagcagac caaccaattc atcaagtggt
     8101 tgcctgcttg tctgaaatgg ctagctggca tgaatgcgga ggtcggctag tatggtagac
     8161 aatgtgtttg aggaaatcgc cttatcttat cgtaggaata cagaacaaca agaagagttc
     8221 tgcgaaaagc ataacatccc tttgataaag atattgagga ccgagagtgt tgtatgtcgc
     8281 atgtgtgaat ctgagcggat tcatgaggaa aatcaagcaa gagtgaatga attggccgac
     8341 gctgagaatg agcgagagag gaaatactat cttgagaagt tttctcttta tgatgaggtt
     8401 ttgaaaaatg cgactttgga caattttgaa acaccaactg aaaaagaagc agaaaagcta
     8461 gtttttgcaa agaggatttg tcgtgagtgg tctgagggtg ctaggaacaa catcgtgtta
     8521 caaggagaag ctggaacagg taagagccat ttggcctttg cgatggttaa ggctctatct
     8581 gagtacacga aagagattgc tatcttcatc aacgtgacgg acttgttgat gaagattaaa
     8641 gctgatttta gtcaggagga gtttctggtc aataaaattg ccagtgctaa gttcttggtt
     8701 ttggatgatt tgggaatgga aaaggatagc gaatggtcgt ttactattct ctacaatatc
     8761 ctgaataagc gttcaaatac aatcattacc accaatttga cttctgctga tattcagaaa
     8821 agatatggca gaccctttat gtccagactg atgaagggtg tggataaaga ccatttaatg
     8881 gttttcaacg acttgacaaa caagcggaag caatattttt agaatggagg tggctgatgt
     8941 ttattttaag acatgggaca agagaggata agccgtttct gaggtccgta gttattggtg
     9001 tgactggctt ggacatttca tgttcagagg agaagaaagc catgcggttt gtttctcgtg
     9061 gggcagccgt acaggttggt aaggctttga ggggttcctt tgggaatttt tatcccgttg
     9121 aggtggagtg atgttagagc tttacttcgt ctacaacggg cactgcaagt tttaccttgg
     9181 aacgtttgac aatgtcgatg atctcattga acagatggaa gatcatcagt gggttttctc
     9241 ggctatcact catccaagat ttcagaagca cattggtcag cggacgacac ggtttgacta
     9301 cggttcgaag gattgttact atttagcgac tttttcagga ggagaaaaaa atgattgaac
     9361 ttattaaaga atttggaatg gctattctgt ggttatttct cggctattta gtcggggaac
     9421 gtgcagcaag aaaggaaaag aaagatgatc aataacgtta catttttagt gggaggtaag
     9481 aaatatggtt ggagtaacct atcaggaaat tcatctcttt gttgaatttt tgaaagagca
     9541 gtatggacaa gggcgtccag actatattga agccctgaac gacttagacg gtctggtgga
     9601 agtctcctac agagaagcta ttgaaagatt tttagaagat gaattatgat aaacgaacag
     9661 tcattgatgg actgaaacgc acaatcgagc aaaacgaaga gaagataatc gagtattcga
     9721 agccgtgtga tgcacgcaag agacggatta gagcgctgga gcgcgatttg ttgaagaaaa
     9781 agaataaaga attaagacgg aaagcggagg agttggaaga tgatggaaga gttaaagaaa
     9841 aaagttaatg cagtatacaa ctggacggta gaagacggga agccgcaacc tccccagcaa
     9901 gatttaccac aagcagtgaa agaccgggcg gactattttt gggaaatggc agaagatggt
     9961 atgacgttta tgggagcgat ggaatgcatc ttcgctgatg aaaagcctac agactatgat
    10021 ttgggagcta ctaagggttg gttgccaaaa tctaaggagt ttgatgattg ggttggctat
    10081 tcgccaggaa tgtctcaggt agttattgca gtttatttga tttatggagg aaactaagat
    10141 gaataagcag gaattgatta aaaaattaga ggaacgaaga acaataactg ggaattttca
    10201 aggttatgta gtttggtgga aggatgtaaa agaaatcttt gaacagctag ccgaatccga
    10261 aataggtcac gcagatgaag ctccacgcta cgtaaagaac atactagcac gattgcgaga
    10321 attgccattg catgatagag aggtttggtt aaaggctatc atgagcgaat ttgaacagga
    10381 ttttagccat gcaaaatggc gagagggcta cgagcaaggt aaaattgagg gtatggttga
    10441 acgtgaaaaa gtcatagttc cgcggtttgt ggctgaatgg attgaggaag ctagaaaagc
    10501 ttgtaaagac gtggtagaat tattcgaatt tgatttcacg aacgatgaag ttagaaaatg
    10561 gtttatgcaa gaaagaccat ttgatttagt agctagagca tggcttgatg gctacgaggt
    10621 cgaggaagag aagcggtatt tggtgacttt aaaaaatagg cagcctttgg tcaaatcgca
    10681 atcagggagt actctttatt ttagtcaaga tataacagct aggaattata aaggtactca
    10741 aaaagaacta gaagacgcaa acttcggctg ggtgtttgat tgtgaaggaa ttgagattga
    10801 ggaggtggag tgatgacaca aacacttgaa gaaggaatga agaatcaaag taaatgcata
    10861 aaaatcccaa tggaaatcag accgtttgat gtgggttatc gaatagtaaa taaacacggt
    10921 caagcgcttg ccttaaaaaa cggagcaagt atattcgctt taccttcgct ggccgaaaaa
    10981 gcgataaaga aagagtttgg aaaaaatgat ccagactttg acattgaaaa acattttgtt
    11041 gaagaggtcg ctattgtcaa tttaagtaaa tttcatagtt actttgaggg ggtggagtga
    11101 tgtcatgtag tgaaaattta aaaaaagaaa aagaattgac tgctgctatt tcaaatttca
    11161 agatagaagt cttacaaaat gatgataaat tgagcagtct atcattaagc aacatcaaaa
    11221 ggcaagcaag ggatctatat gaatgcttag tatggttgca gtataatgcg gaggaatcag
    11281 gtagatgagt tatgatttgg aaatattagt aaaaatagag agtggagatt atatttgtat
    11341 cgctgaacct aaatatagtt ctccgaccta caatcttgga agaatgttta gagttgctat
    11401 gaactgggat tttgatcaag acactacgta caacatcgct gatgttttag ataacattca
    11461 acgcggtatc tctgaactag aacggtaccc tgaaaagtat gtgcagtatg aacctgaaaa
    11521 tagatgggga acagttagcg ttgcattgga ggttttaaag tcactgaaag agtgtatttt
    11581 agaacaagat attgatacga aatatttata tatgaggtgg taatatgaga cgattcatag
    11641 ctatctggat tcttgtctct gccggattga acatctggca tatggacagg attcgagatt
    11701 tggaagagaa aaagccgatg gttgtctata aagctgataa cgctggcgct gagatattcg
    11761 ggcgtgtcgt tgaaaaagga cggcatggga agttgtatac agtaactatc agagactatg
    11821 ggattttcgt agttacgaaa gagcagtttg agaaaatcag agtaggggat gaggtgttac
    11881 tctaatggat gatattttac aagctttagc aaaaatgcta aatatgactg ttgatgaagt
    11941 aagttctttg cttacaacat ttaaagggaa tgcaccacag atttatgaaa tgttcgttaa
    12001 agaaaagatg ttttatgatc tcttcagtct ttttcaaatc atgtcaattg taatatttag
    12061 tatttctgca gtagttttag cagttttaac tctcatatat tttacatacg atggtggttt
    12121 tgtttattcc tatgatatac gtacaggaaa aaccgaggaa gaaattaaat tagaacgcat
    12181 tgaacggaaa agaaaggact taaaaatacc actaaaaatt agttgcattt catcaagcgc
    12241 aagtttgata acattagtta ttgcaattgt tttaaaagca actcttgcac ctaattatat
    12301 attcatcgtg aatgagattt taccaaaatt aacgaagaga taggagttat catgaacaca
    12361 atagagaaag tcaaacaatg gtttattgac cgtgatttag aaaacggtag ggtcgttgga
    12421 ttgatggtgc tttcgtcaaa gaggaggatt tataaaatga aaagactagg aatcattatt
    12481 ggggcggtat ttgtaatcgt tgtatcgcca tttgtagttc agtatggatg gaatgagatt
    12541 atcacaacaa ttgttccagt tggtaaaatt acagtctggc aagcattagg gatggatgca
    12601 ctactatctt tcatctggcc tgtgttatct agcaaaaaag aatctgaaga ggattattcg
    12661 tatgcggtaa agagcagtat ttcaaaaatc attacatgtg catttttgat atggttagct
    12721 agtttgttta tttaaggagt gttagcatga taccaaaatt tagagggtta tccattgacg
    12781 aaaaaagcaa aggagaatgg caatacggac atttaattga agatagagga agagcattta
    12841 ttatcaacga agtggtagaa gccaatgaac aatacattac tataggttct tggtgtcctg
    12901 taaatccagc aacattagga caatcaacag gactcagaga caagaacggc aaggaggtat
    12961 tcatcggtga catcgttaaa tgtacaagag gatgtctcca tgaagtatat ttagaaaaag
    13021 aatacggtgg cacattcata ggcggaatgc cttccatata tctaaaggga ttgctaagtg
    13081 ggtatgcgtg gactgaagac gaggaaatca tcggcaatgt ctacgaaaat ctagtttaca
    13141 ttttttaaaa acttggaggt ggaaattgaa aaaattgagc gacgaagacc tcaaaacatt
    13201 agacagagaa cttttcaaat ttcaaaacat tcaacgtaca atagatttga gaaggctaga
    13261 attagaaact cgaaacccag atgctcagag tagtcccagc gtaggaataa gcaaacctac
    13321 cgaaactatc gcagtcagaa tcgcagatga tccaacctta aaatttctcg aagggttcaa
    13381 agctattatt aacaaactct tgatcaatct agttgatgaa gataaggaaa tctttaatct
    13441 gcgctggaga tatcctcaac tgagatggga agaaatagca gaacagaaat tcatgagcaa
    13501 agctacaatc tatcgacgta gaaggattat cttagaacag tacgctatac tgaaaggtga
    13561 gttgtaaata agattgagac aaaagacatc ttgaagtctc ataaaaaaag gtctattatg
    13621 atagcatgaa cttctgaaac aaaaacacac atcacacttg aggagtcatc cttaattcta
    13681 gtccaaaagt tgtccaacag aagtatcgtc aagagtcagc aaatgctggc tttttgtttt
    13741 tggaaaggag gtagaatatg gaatttgtat caccgataaa agataatgac gacattcagg
    13801 caatgaaaga ttatcttaga gagtggaatg agatgtatta tatgctattc attacaggcc
    13861 tgaatactgg tttgcgagtc ggagatatac ttaccttgaa agttaaagat gttcaaggct
    13921 ggcacatcaa actgagagaa cggaagactg gcaagcagat aacaagacgg atgacaaaag
    13981 aactcaagaa agaaatgagg agatatgttg agggcaaacc atttcatcat ttcttattca
    14041 agagtaggca aggtcagaat aaagcaatca ctcgtgagcg agcctatcaa atcatacatg
    14101 aagcagctga agaacttggc attgataatg ttggcacaca tacaatgcgc aagacattcg
    14161 gctataaata ttacaacaag acaaaggacg tagggacatt acagaaaatg ttcaatcact
    14221 catcacctgc aattaccctg agatacatag ggatagaaca agcagagctc gatgatgctt
    14281 tacggaactt tgtcatttaa ttttttagat attactttca cataatgagt taagcataaa
    14341 ctgaaaaaat gaaactcttt aaaacccatg cttagtaagg gtttgagatt tagagtgagt
    14401 ttaacaaaat ataagatatg tgaaagtgag ggataaaatt ggtatagtta caagaggtga
    14461 aaaatggtaa aagaataccg tatttgtaaa tggaatgcat ataccgttaa cagccatact
    14521 agtaaaatgg aataattttt ttaaagaatc agaatgagac aaaagacatc ttgaagtctc
    14581 acaaaaaaag gtttattatg gtagcataga tttcttgtat gaggagggga taggtcaaag
    14641 gcctgtccct tttagcattg agaaaggagg tttgagatgt ataacaaacc tatcagacca
    14701 tccttgaaat ctaagaagtg ggagaagttc cgtgatagga taatgcgtaa gcatgattat
    14761 ctttgccaag aaagtttgcg ttacggaatt tctgttcaag cagaaatggt acatcatatc
    14821 tttcctgtgt ctgaatatcc tgagcttgaa tttgttgaat ggaattgctt gccattgacg
    14881 aataagaaac ataatacgtt tcatgataga gtgaacgata gagtaatcaa ccaaggattg
    14941 tattggcaga aaaaaagaaa aaaagaattt ttaaattttt tcaaaaatga aaaatgaaaa
    15001 tttttaatcc cccctctttt tgaaaaatca ttttggccag tagggtaccg gtgaagggaa
    15061 ctttttccaa gtcgggggcc ttcaaacaaa aagggggtaa aaactaagcg attttgacga
    15121 aaggaggtag tttttggcta aaccaattac agcaaagtcg attaagtcaa aagtggtcaa
    15181 gcagatgaaa gacttgggaa cttatcgtaa agagttcgaa atgatcattg acatttttgc
    15241 aggtatgtta taccagtatc agaaacttgc tcaagattat gctgatatgg gttatccagt
    15301 aacagacacc tacgtcaata aggctggtgc tgaaaatgag cgcaaagttc caatcttgac
    15361 agcgatggaa attttgagga aagacatact cagctactct aatcagttga tgatgaatcc
    15421 taagtcgctc ggtgaggtag tagaacaaga gggtgagtca gttcttactg aggtactgaa
    15481 gttcaagaac gaaatcaaga agaagcgagt gactggcaat gggtaatctt gataaagcta
    15541 aaaaatacgc tcaacacgtc ttaactcacc gagaagaaca ttgcgaagaa aacatccttg
    15601 ctgctgaacg ttttttccgt gatttagaaa atccaacctt tgagatggat gaggatatgg
    15661 tggattttgt tattcacttt atcgagaacg tgatagttca tcagcagggc gatgatatgt
    15721 ttgcggtatc tatccgtaac aagccattgc ttttgcaacc gtggcaacat ttcgttgtag
    15781 ttaatctgtt tggtttttac tacaagggta cgaatgagcg caggttcaaa gaagcgctca
    15841 tcatgcttgc tcggaagaat gggaagacct cgtttactgc tgcaatcgca cttgcttatc
    15901 agatattaga tacagatagc ggttccaaat gctatatcgt tgctaactca gtcaagcaag
    15961 cgatggaagc ttttggtttt ttaagattca acgttgaacg ctggaacgat aagaacattc
    16021 gtatcaagga taataaccaa gaacactcta tcaccgccaa ttttggtgat gaaggttctt
    16081 tctttatcca ggctttagcg aatgatgaga gccgtctgga ctctttgaac ggaaatgtta
    16141 tcatcttgga cgaagctcac acaatgagga acagtaagaa acatggtctt atgaaaaaaa
    16201 caatgtcagc atacagaaac agtatgcttt ttgttatctc aacggctggg gatattccta
    16261 ccgggttcct tgctaaccgt ctgaaatact gtcaaaaggt gctcaagcaa ttagtcactg
    16321 atgattcatt tttcatcttc atctgcaagg ctaatcaatc tgctgatggg gacgtggtga
    16381 actatctgga cgagaatatc ctcaagatgg ctaatccgtc atggggtgtc acggtttcgc
    16441 tcaaggctct caaggaagaa gcagagcagg ctatgaatga tcctcagaca agaaatgagt
    16501 ttttcaataa gaccttgaat atcttcacta actctatgaa cgcttatttt aatcctgatg
    16561 agtttattgc gtcggatagt tgctacgatt ggagtttaga agagctggca cgtttgccta
    16621 ttcgttggta tggtggtgcg gacttgtcaa gattgcacga cttaacagct gctgctctct
    16681 atggtgtcta tcatgacggt gagaaagacg ttgatatctg tatcacacat gctttcttcc
    16741 ctcggattaa tgctcagaaa aaagctaatg atgacgggat tccactcttt gggtggcagt
    16801 ctgacggttg gttgacgatg agcaacactc cgaccgtcct ctatgatgat atcgtcaaat
    16861 ggttcatcaa gatgagggag aaagggttca agattgctgc tgtcggaatg gataggaagt
    16921 ttggccgtga gttcttgacg aagatgaaac aagctcggtt caagatgatt gaccaacctc
    16981 agctttttta tctgaaatca gagggattca gacggattga gtttaaagtt aagaataaag
    17041 aattttacta tcttcattct gatgcttacg aatactgtgt gagcaatgtt agagcaattg
    17101 aaaaggtgga cgatgctgtg caatatgaga aattagatgg agacggtggg actgcaagga
    17161 ttgacttgtt tgatgccagc gtctttgctt gtatacaggc tcttgctaat cttggcaagg
    17221 gtggtgatgt gatgagattc tttgattagg tgaattatga atgaaatagt attatcagaa
    17281 catgaaatta acttgctgat caacaaaggg cgagttaaag taattttaaa cggggaagta
    17341 gtaactattc gtcaaagaca tatgaaaaat ttgatggctg aaacagtaaa atgggaaaaa
    17401 caggtaattg atgtcagtca gaatatcgta agaaataaac actttgattc actttttcaa
    17461 aatacttttc gttagaaagg aggtgaggaa acatgggtat ttttgaaaag ttttggaaac
    17521 gaaacaagcc aagtaagtca atcaacatgc tgagtcattc agatttaggg ttgtcaaacc
    17581 tgatggactc gtatgtacct ttggccagaa atccagatgt ggtgacagcg gttaataaga
    17641 ttgctgattt ggtctctaat atgaccatcc acttgatgga gaatacagat aaaggtgata
    17701 tcagaatccg tgatgggctt gctagaaaga ttgatatcaa tccgtgtaaa cacatgacaa
    17761 ggaagtcatg gattttcaag attgtgcgcg atttgctttt atatggcgat gggaactctg
    17821 tcctacatgt ggaatatgaa cctgttacgg attatatttc taatctaaga ccatttccga
    17881 tgagagaggt ttcattccaa acagataagg attcctatgt aatctcattt aggggtgaag
    17941 aatattcccc tgatgaagta gtccacttcg tcatcaatcc agatccagat attccataca
    18001 ttggtactgg ttttagggtg acgttgacag atgtggttca aagtttgaac atggctacca
    18061 agactaaaaa aagctttatg aacgggaaga atattcctag tcttatcgtt aaagtagact
    18121 cgtctagtgc tgaactagat tcggagcaag ggcgtgagcg tatcgctgag aagtatttga
    18181 gtactagcag ggttggcgct ccatggattg ttccagaggc attgctggac attcagcagg
    18241 taaaaccgct tagtctaacg gacatcgctt taaacgagtc tgtggaattg gataaaagaa
    18301 cagttgcagg tctattagga gtacctgctt ttattttggg tgtaggagag ttcaacaaga
    18361 cagagtataa caactttgta aatacgactg tcatgagtat tgctaccact attactcaaa
    18421 cactaaccag ggacttactt ttgtctagca atcgttactt caagctaaat cctcgttcac
    18481 tcttctctta caacattacg gagttgtctg ctgttgctca acaaatggca aacagtgctg
    18541 caatgcgtcg taatgagtgg agggattggc tagggatggc tcctgatcct gagatggaag
    18601 agttgattgt tcttgaaaac tttctccctc aggagaaact aggagatcaa aataaactga
    18661 agggaggtga ggaagagaat gcaaaagcga aatagctatc gtgccactca atttcaaacg
    18721 agagaagaag aatctggaga cttggtgttg agtggctatt ttataaaatt tgatgaagag
    18781 acggagttgt ggcgtggtta tcatgaagta atcaagcgtg ctggtgttga gaaagctgtc
    18841 acagacgctg atatcagagc tttatttaac catgatgata gccttgttct cggtcgaaca
    18901 ggtaacggaa ctctgacact gggtgttgat gatgttggtc tttttggaga tatcatcatt
    18961 aacaaggatg atcctcaagc tgttggggcc tatgcccgtg tcaagcgtgg agatgttata
    19021 gggtgtagct ttggctttat cccggtaaaa atcgaaacgg aagaacgtga agatggttca
    19081 tatttggaca ctgtcttaga gctagaaatc tttgaagtga gtccatgtac tttcccagcc
    19141 tatccacaaa cggaaattgc tgcacgacaa aaagacttcg aaagtcagaa gcgtgctaat
    19201 cgtgaagcgc tagacaagcg caagaaagaa attaaggaga aatttaagct atgaataagg
    19261 cattaatctt tggtgctcgt atgcgagcaa aagcaactaa ggtagttgag ttggaagaaa
    19321 ctatcgaaga attgaacaaa cgttcggttg ttgagttaga aaagttggat cgtgctaaaa
    19381 atgatgaaga agttttagca gttgaaaaga ctgtagacgg tcttcaaagg gaaattgaag
    19441 aaaaagaagc tgaaaaagta cagcttgaaa acgagatcga tgagttagat aaacaaatca
    19501 aagagcaaaa tcgtaaagca ccaactccgg ggaaaatgga agaacgagga ggaaagacat
    19561 tgggacaacg tgaagcattt aatcattact tacgaacaaa agaagcgcgt gctgatggtt
    19621 tcaaatctgc cgaaggggaa gcaatcattc ctgttgaatt gatgacgcct aaagaagcga
    19681 aacaagacaa gacagatttg acttcattgg tcaacatcgt taatgtcaag aacgcaagcg
    19741 gtaaatggtc agttgtaaaa ttgactgatc aagaaatgaa cactgttgaa gagttggaag
    19801 aaaaccctga attggctaaa ccaaccttta caaaggtgaa ctatgaaatc caaacacgtc
    19861 gtggtcattt gccagtatct caagaattta ttgatgacgc tgactacgat gtcatgggat
    19921 tggttgctaa acaaactaag aaccaagaac gtatcactaa gaataaagag atcgctaaag
    19981 ttctcaagac agctacatct aaaagcgcag ctggtttgga cggcttgaaa gatattctca
    20041 acgtggaatt gaaaacatac tacaatgcaa ctatcgtatg tactcaatct atgttcgctg
    20101 ctcttgataa aatcaaggac aaggacggtc gctacatgct ccaaacagat atcacatctc
    20161 caactggcta caagtttgct ggtcgtgtta ttgttgttta tccagatgat atcattggag
    20221 aaagcaaggg agatttgaaa gccttcatcg gtgacgttgg agaatttgcg acattgtttg
    20281 accgtgcgca gactactgtc aaatggcaag atgacaaaat ctacggtcag tatttggcaa
    20341 ccgctaatcg ttttgatgta gtaaaagtag acggagatgc aggattttat gtgacctata
    20401 cagatgctgt tttgtaagga ggtagtaaat ggtatataag gtaatccgtc cttttaagga
    20461 cttacgagac ccaaagaaac attgctataa ggttggggat aattacccta gagttaagca
    20521 caaacttgat aaagagtttg cggagactct gctaaatggg gcaaatagtg caggatctat
    20581 ctttttgatg aatgtagaag ataaggatat gtcgaaagat gacacattaa ctacagaagt
    20641 agaaaaggaa gaaacaaagg tagtagatgg gaaacctgaa gagggagacg aggagtagtt
    20701 atggacaatg ttcaattatt agaattgctt aagctaaaat tgggtatagc aacaaaacta
    20761 cgcgataagc ccttggagaa aatcatcgaa gctgtcaaaa cagaactaga agataactta
    20821 ggagttctac ttgacttaga cagctcagaa gaccaaatgt ttgtagttga ttttgcagcc
    20881 tttcgctatg agggtggggt ggatatgcca cgtcaccttc agtggcgact gcataattta
    20941 cagatagcat caaagaaaga ggtcaagaat gtggaatcat gaaatcacac tgatctctaa
    21001 ggaagtcaca ggtaaggata aactactaca accaatctct gaagatgttg aagttactct
    21061 cctatgtcgc aaaaagaggg tcactcgctc tgaattttat caggcgaatc aggtagggct
    21121 taaaccgagc ttggtcgttg agattcgaaa ttttgagtat gagaatcaag agcttgcgaa
    21181 gttcgaaggc aagcaatatc atatcttgaa aacctatcct attgattctg aaattttaga
    21241 gttgactttg tcagagatgt tgaaatgagc ttaacaagtg atttagcgaa tgaaattgca
    21301 aaggcaatgg cagagtactc tgctgaggta gaagataaga ttgacctgat tgctgaggac
    21361 gttgtaaacg aagccgttac ggaattaaaa gcgactagtc caaaacgtca tggaaagtat
    21421 gctagaaatt ggcgcttcaa gaaaaatgct aaggggtcat acgtcatcta caacgcagct
    21481 ccaacctatc gtttaactca cttactagaa aatgggcatg ttttgagaaa tggcggtcgt
    21541 agtcgggcat ttccacatat taaacctgtt gaggagaaag ttaaagaaaa ctttgagaag
    21601 cgaatcaagg agattggaaa atgaagctat cagactttgc agctattttg gaacaggcaa
    21661 acttgcctgt cacttatcga gcgtttaaaa ctgggaacgc tcctgaccta ccttatctgg
    21721 tctattatga atcaattcca gccatcaatg cagctgacaa cacggttaat catcagatta
    21781 agagcgtgac agtagagcta gcttttgaga gtaaggatga agatttggaa gaacgtctgg
    21841 aagagctgtg gacaacccac gagctctttt tcgatgttca agaagaaaca tttatcgaga
    21901 ttgaaagact ctatgtcaag tcttatacag tctatctata ctaaggagga atgacatgaa
    21961 tcaagaaaat aaagtaactt ttggtttaaa aaatgttcac gttgcgccaa ttaaatcaat
    22021 tggtgcagat ggagtgattg cttacgatga aattttccgc tttcctggag caatggaatt
    22081 gacattggat ccaaggggtg aatcaatacc aatcaaagca gacgatatcg attatcactt
    22141 catgaactca aacgaagggt atgaagggaa attcaaaatc tctcacatta ttgaaatgtt
    22201 tgcgactaag attttgggtg aaatcaaaga tgctcagacg ggtgttttga ctgaaaaagc
    22261 tgatgcagaa ttcacatcat ttgccttgat gtttgaattt tcaggggaca agaataaaac
    22321 acgtcacgtc ctttactact gttcagcgag ccgtccaggc aatggttcaa aaaccaaaaa
    22381 tggtacaaac gtcaacgagc gtgaacttgg ctttaaagca agtcctcgcc ctctggattc
    22441 agttgttaaa cgttctatca catcagctga taacaaggaa atttatgaca actggtttaa
    22501 gaaagtgtat gaacctactg cggtggcagc ttaaggagaa aatctatgcg taaaatcgtt
    22561 tgggttggcg ttcaggagta tgagttgggg accaatggct atactcctat cgcctacaag
    22621 caacaatttg ggaaagatta ttttcaagat ttgttttcaa tgttgaaaaa tcaatcattc
    22681 atgaatgaat tgaacaagct ggaaactgac aaagagttga ctgcaactga tattgacatt
    22741 tcgatgctgt cagattttga tatgacattt ttcaaccgtc ttttttggac ctttgctaaa
    22801 tctgcaaatc ctcacatcaa gccttatgaa caattcttca tggaaatgga agtctttccg
    22861 attcaggaag ttgggcctgt attgatggaa atgctgaatg cgagcatgac gacaaaaaag
    22921 caccagatga atcagaatca gctagcgaag aaatcttcac agtagaatcc tacttatctt
    22981 gctgtaaaga aactggtctt tctattgatg atctaaagca catttcaatc ggaatggctc
    23041 tggattatca gacggattat gtgaatctac ggagcgagga taagggtggc gaacggaaag
    23101 ccacgcaagc tgattttgac agtttttaaa gaaaaatgag tgctgagaga gcgattctga
    23161 gaccaagttc cttggtctgg ctgcattatc agtcgtagaa gttctctcag cgcttttcta
    23221 ttttttgaga aaggaggaaa tatggcagga aatatcaaag gtatcaaaat tgaaatcgat
    23281 ggcgacacgc aacccttgca gaaggcgctg aaaaatgtca ataaggccgc tactgatgca
    23341 agtcaggagt tgagacagat tgacaaagcc ttgaaatttg atacagggaa cgtaacgctc
    23401 ctgactcaga aacaagaggt cttacaaaag caagtttcga cgaccaaaga gaaactagaa
    23461 accttgagac aagctcagtc tcaggtggaa cagcaattca aaaatggcga tatcggtgct
    23521 gaccagtacc gtgctttcca acgtgaagta gaagttactc aaaacgtcct aaaaggatat
    23581 gagggtaagc ttgcaaatgt gaatcaggcg cttaatgaga atgggagtgc tactcagaac
    23641 aacaagaacc aattaaaaga gttgcaaaat gagcagagtc aacttgcttc agagatggta
    23701 aaggtgacaa gctcattcaa actgcaagaa agcgctttgg gttcaaatgc tagtgaagcc
    23761 gagagaaatg ctcttgctca gaaaaagatt ggtgttcagt ctgagattgt aagtaaacag
    23821 atttcaaatc tagaacagca attggaaatc actaaaaaag aatttggtga gaactccaca
    23881 caagccaaca ggatggaagc tgagctaaat caggctaaga cagcattcaa tcatctcaat
    23941 gatgagatga aaggaacaaa gtctgctgct gatagcactc aagaaagttt aagtgaaatc
    24001 tcaagaaatt taagagcaga actacttcaa cagtttagtg agaagttgag tgctatttca
    24061 gaaaagcttg tggaagtagg aaaagaagcg ttagaagcag ctgctcaaat gcaagctagt
    24121 aatgctcaat ttactaccgt tttcggagat atggaaaccc aagcaagaga agcgttgaat
    24181 gctattggtc aggaaatgga tattgttcca gagcgattgc aaggttcgtt tacacagatg
    24241 gcttcatttg ccaaaacttc aggcttggac accgctgaag cattggatct tacttctcgt
    24301 gcaactaggg cagctgcaga tggtgcagcc ttttatgaca agtctattga gagcgtgaca
    24361 gaaagcctac aatctttttt gaagggaaac tttgctaacg atgcggctct tggtatctct
    24421 gcgacagaga caactaggaa tgccgctgca aatgaattgt acggaaagtc attcaaggac
    24481 ttgagcgaag cgcagaagca attgaccttg cttcagatgg tcgaagacgg aaataaactc
    24541 tcaggagctc ttggacaggc tgcaagagaa tcagacggcc tagaaaacgt gatggggaat
    24601 ctgaaacaag ctgggaccaa tgcattatct gctattggtc aacctcttct agaaatgatg
    24661 attcctgttt ttcaaacctt ggcaacgatt gtgaaaggtg tggccgagct gttcaattcc
    24721 ttgcctgatc cagtaaaaga tttcattgtc atcttaggtg tggctttgac aattgtagga
    24781 gccttagccc ccatattctt aaccctgcaa gctgtgttta tgtcctcatt tggcgcaatg
    24841 attgcagcag cattaccaat cattggaatt atttcaggag ttgtagtggc catagcagcg
    24901 attgttgcta ttgtgaaata tctctgggaa actaacgaag gttttcgaga tgcggtcacg
    24961 accgtttgga atgcgattct tgaagttatc aatgcagtcg tatcagagat ttctaatttt
    25021 gtcatgagta tctttggaac ggttgttgct tggtggacgg agaaccagga acttattcga
    25081 gcaagtgctg agactgtctg gaatgctatc tatacggtta taagcacaat tctggaaatt
    25141 ttaggtccac tcattcaaac tggttgggat aatatccaac ttgtcattac aacagcttgg
    25201 gaaatcatta agactgttgt tgagactgca ataaacgttg tccttggtat cattcaagca
    25261 gttatgcaga tcatcaatgg tgattggtca ggcgcttggg aaactattaa gggggtattc
    25321 tctactgtat ggcaagctat ccaaagcatt gtccagacca ttttttcagc tatccagagc
    25381 tacatttcaa atgttctcaa tggtatttca ggaactgtat caaatatctg gaacggcatc
    25441 aaggatactg tctcaaatgt gttaaatggt atatctggca cagtatcaag tgtttgggaa
    25501 ggtattaaga gtaccatctc aggagctatc aatggtgcaa gggatgctgt atcttcagct
    25561 attgaagcta tcaaaggatt gtttaacttt agtatcagct ggccacatat cccactacct
    25621 cacttttacg tgagtggttc ggccaatcca ttagattggt tgagtcaagg tgttccaagt
    25681 attggaatcg aatggtatgc caaaggcggt atcatgacga aaccaactat ctttggaatg
    25741 aatggtaata acataatggt tggtggtgaa gctgggaatg aagcagtatt gccacttaac
    25801 gacaaaacgc ttggagccat cggtcgaggt atcgctcaaa ctatgggtgg aacttcacca
    25861 accattaaca ttaccattac tggcaacact gtcagagaag aagccgacat cagtcgtatt
    25921 gctgatgagg tggctcagcg tattgctgac gagttgcaac gtaagacaca attgagagga
    25981 gggtttacat gataaagcat aatgagcttg tgattgacgg tgtgagaaca tcgtcttttc
    26041 cgtttaaagt cattgtccat gattctcctt caattactct gggagagagc aagacagctc
    26101 ttttggagca tggtggtatc agtggagcaa tcgttcagac gaacaaacat agggaattgg
    26161 tcaagaaaac ctatacgatt tacttggtca aacctactga agaacagatg aaccaattta
    26221 tgagtctgtt tatccgtgaa aagttctggc tagagagtga gcgagtcaaa acaactcgtc
    26281 tttggtgcta taaggtcaat gtgagcgacc ttgaagaagt gcaacctggt ctttacatga
    26341 ccaaagcaac cttcacttgc catcctacaa aatactttaa aggctccgat acacagagat
    26401 tgacaagaag tggaaccttg accgttcaag gttctgctct tgcttttcct aaaatcacaa
    26461 tcgttggtca gagtgctgtt gagacttcat ttacaatcgc tggtcaggtc attaggcttg
    26521 aaaaactctc agaatcgctt gtgatggtta ataatcctga caatcctagc tttaaaacga
    26581 caacagggaa gccagtcaaa tggtcagggg attttatcac agttgatcca gcgaaagtta
    26641 agaatattgg ggttgtttta ggcccaggta ttcaatcgat tgaaatcgaa acggtttggg
    26701 ggtgggcata attgctttat ctacttaata aagatgtaag aaccgttcga tggaacgggg
    26761 agccacttca tgaagcgact tcggcgattg ttaaagagat catgaatggt gatttcacct
    26821 taactgtgaa atatcccatt tccgactctg gtatttatca gctcatccaa gaagatatgt
    26881 tgataaaagc gccgactcct gttcttggtg cgcagctatt tcgcattaag aaacctgttg
    26941 aatacaatga tcatctggaa atcacagcct atcacatttc agacgatgtg atgcaacgtt
    27001 ctatcacacc agtaagtgtg actagtcaga gttgtggcat ggctctttct cgcatggtac
    27061 aaaacaccaa aacggctctt ggagacttct cattcaacag tgatatccag gaccgtagga
    27121 ccttcaatac gactgaaaca gaaaccctat actctatatt gctggacggt aagcacagca
    27181 ttgttggtac atgggaaggc gagctggttc gtgataactt tgcgataact gtcaagaaga
    27241 gtcgtgggga gaatcgtggt gttgttatta caacgcacaa aaatctgaag aactaccaac
    27301 gcacaaaaaa cagtcagaat gttgtcacaa gaattcacgc aaagtcgact tttaaacctg
    27361 aaggtgctga aaaagaaacg actatcagag tgactgttga tagtcctctt attaattctt
    27421 atccttacat aaacgaaaaa gagtatgaga acaacaacgc aaaatccgtt gaagagttgc
    27481 agaagtgggc acaggctaag ttctcaaatg agggcattga caagatctct gacgctatca
    27541 agattgaagc ttatgaactt gatgggcaag ttgttcacat gggtgatacg gtcaatctca
    27601 agagctggaa acataatgtt gatgtattta agaaagctat tgcttatgag ttcgatgcct
    27661 taaaagaaga atatatctct ctgattctcg atgataaggc aggagctggt ggttctagaa
    27721 catctggtgg cttatctagc gcagcggatg ccattcttgg agtaacagaa tctgcacaag
    27781 aagttgccct tgaaaaggct cttcaaaatg ctgacttaga ctttgatcat aaggctggat
    27841 tgcttagaca ggaaatttct gacggcatcg aacttgccaa ggccaaggcg gaagaagtca
    27901 agcaagaact gtctgacact atcaatcagc gctttaatag ctttgacaac gggccattga
    27961 aagaagctaa gcgcagggct gaagaagcct tgagaaacgc tggcgcaagc agcttactcg
    28021 ctcaggaagc gaagcggatt gggctggatt ctgtcgctag acttgaagag tttaagtcgc
    28081 agactacgag cgcacaaacg gctctgtcag gtgacttgga cgctctgaaa cggactatcg
    28141 tgaatgatat tcgaccgaag caagcacagg ttgaagctga gattgccaag caagttgaag
    28201 cacttgttca gaccaaaaaa gaactggctg gcgcaagtac cct
//
LOCUS       DAVMEF010000005        27884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMEF010000005 DAVMEF010000000
VERSION     DAVMEF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.005"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            108..1052
                     /locus_tag="VYQ30_000150"
     CDS             108..1052
                     /locus_tag="VYQ30_000150"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1965324.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRQIEDDASAYGYL"
     gene            1061..1738
                     /locus_tag="VYQ30_000151"
     CDS             1061..1738
                     /locus_tag="VYQ30_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1965325.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            1836..2594
                     /locus_tag="VYQ30_000152"
     CDS             1836..2594
                     /locus_tag="VYQ30_000152"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1965326.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            2632..4137
                     /gene="lytC"
                     /locus_tag="VYQ30_000153"
     CDS             2632..4137
                     /gene="lytC"
                     /locus_tag="VYQ30_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1965327.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            4634..5152
                     /locus_tag="VYQ30_000154"
     CDS             4634..5152
                     /locus_tag="VYQ30_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1965328.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            5282..5788
                     /locus_tag="VYQ30_000155"
     CDS             5282..5788
                     /locus_tag="VYQ30_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1965329.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFGPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            5990..7222
                     /gene="clpX"
                     /locus_tag="VYQ30_000156"
     CDS             5990..7222
                     /gene="clpX"
                     /locus_tag="VYQ30_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1965330.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            7231..7818
                     /locus_tag="VYQ30_000157"
     CDS             7231..7818
                     /locus_tag="VYQ30_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1965331.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            7829..8209
                     /locus_tag="VYQ30_000158"
     CDS             7829..8209
                     /locus_tag="VYQ30_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1965332.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            8261..9151
                     /gene="rapZ"
                     /locus_tag="VYQ30_000159"
     CDS             8261..9151
                     /gene="rapZ"
                     /locus_tag="VYQ30_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1965333.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            9148..10125
                     /locus_tag="VYQ30_000160"
     CDS             9148..10125
                     /locus_tag="VYQ30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1965334.1"
                     /translation="MRKPKITVIGGGTGSPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            10122..11033
                     /gene="whiA"
                     /locus_tag="VYQ30_000161"
     CDS             10122..11033
                     /gene="whiA"
                     /locus_tag="VYQ30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1965335.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(11067..12032)
                     /locus_tag="VYQ30_000162"
     CDS             complement(11067..12032)
                     /locus_tag="VYQ30_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1965336.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            12168..13025
                     /locus_tag="VYQ30_000163"
     CDS             12168..13025
                     /locus_tag="VYQ30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1965337.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            13012..13791
                     /locus_tag="VYQ30_000164"
     CDS             13012..13791
                     /locus_tag="VYQ30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1965338.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            13815..15167
                     /locus_tag="VYQ30_000165"
     CDS             13815..15167
                     /locus_tag="VYQ30_000165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1965339.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            15241..15615
                     /locus_tag="VYQ30_000166"
     CDS             15241..15615
                     /locus_tag="VYQ30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1965340.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            15617..16465
                     /locus_tag="VYQ30_000167"
     CDS             15617..16465
                     /locus_tag="VYQ30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1965341.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            16731..17498
                     /locus_tag="VYQ30_000168"
     CDS             16731..17498
                     /locus_tag="VYQ30_000168"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1965342.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            17510..18694
                     /locus_tag="VYQ30_000169"
     CDS             17510..18694
                     /locus_tag="VYQ30_000169"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1965343.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            18691..20562
                     /locus_tag="VYQ30_000170"
     CDS             18691..20562
                     /locus_tag="VYQ30_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1965344.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHNLKKELSGGVAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKTFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIEALEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(20680..21864)
                     /gene="mntE"
                     /locus_tag="VYQ30_000171"
     CDS             complement(20680..21864)
                     /gene="mntE"
                     /locus_tag="VYQ30_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1965345.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYNIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            22168..24864
                     /locus_tag="VYQ30_000172"
     CDS             22168..24864
                     /locus_tag="VYQ30_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1965346.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFTQRKLGFDKNAI"
     gene            24877..25668
                     /gene="yghU"
                     /locus_tag="VYQ30_000173"
     CDS             24877..25668
                     /gene="yghU"
                     /locus_tag="VYQ30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1965347.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQVGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            25784..26194
                     /locus_tag="VYQ30_000174"
     CDS             25784..26194
                     /locus_tag="VYQ30_000174"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1965348.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            26206..27801
                     /locus_tag="VYQ30_000175"
     CDS             26206..27801
                     /locus_tag="VYQ30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965349.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT         8117 a         5293 c         6498 g         7976 t
ORIGIN      
        1 taattgaaaa ctatatcttc ttgcagtata ataaaaggac taagtgtttg agatttgtct
       61 tcaaacatat gcaattattc ctgaaagagt acagttagga gagggttatg ccgattcgaa
      121 ttgataaaaa attgccagct gttgagattt tacggacaga gaatatcttt gtcatggatg
      181 atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat tttaatttta aatctcatgc
      241 cacagaaaat ggtcacagag acccagttgt tgcgccactt ggctaataca cccctacaac
      301 tggatattga ttttctctat atggagagcc accgttctaa aacaactcgt tcagagcaca
      361 tggagacctt ctataaaact tttcctgaag tcaaggatga gtattttgat gggatgatca
      421 tcacgggtgc tccagttgag catttaccat ttgaggaagt ggactattgg gaggaattta
      481 gacagatgct tgagtggtct aagactcatg tctattcgac ccttcatatc tgttgggggg
      541 ctcaggctgg gctttatctg cgctatggtg tagaaaaata ccagatggac agtaagctat
      601 caggtattta tcctcaggac accctaaaag agggtcacct tctatttaga ggctttgatg
      661 atagctatgt atcccctcat tcacggcaca cggagatttc taaggaagag gtcttaaaca
      721 agaccaatct cgagatttta tcagaaggac ctcaggttgg ggtttctatt ttggccagtc
      781 gtgatttacg agaaatttat agttttggtc atttggagta tgaccgtgat accttggcaa
      841 aagagtattt tcgagatcgt gatgcaggtt tcgacccaca tattccagaa aattacttta
      901 aagatgatga tgttaatcag gtaccttgtc tttgttggtc ttcatctgca gccctctttt
      961 tcagtaattg ggtaaaccat gcggtctatc aggagacgcc ttttgattgg agacagatag
     1021 aagatgatgc atctgcatat gggtatttat aagaggaatt atgacatatt tagacgcttt
     1081 taaatcaggt accttggttt taccgagtgc cctgctcttg cattttaagg aactctttcc
     1141 ttctagcgac gattttctgg tttggcaatt tttctatttg caaaatacga caggcttaga
     1201 agaaatgtcg ccaagccaga ttgctgaaag gattggcaag gaaatttcgg atgtcaacca
     1261 gtccatttct aatctgacgg aaaggggact gctccagtat cgtactatcg aattaaatgg
     1321 cgaaattgaa ttgttctttg atgctagttt ggccttggaa cgtttggatg acctgtttgg
     1381 agcagttcgt tcaagttcag accagctaac acctcaaaac cagctcaagg atttggtgga
     1441 aaccttccag caggagttgg gacgattgtt gacgcctttt gagattgagg atttgaccaa
     1501 gacactaaag gaagatggaa ccagtgctga cttgattaag gaggctcttc gtgaagctgt
     1561 tttgaatgga aaaccaaact ggaagtacat tcaggcaatt ttgagaaact ggcgccatga
     1621 aggaatcaag agtgtggctc aaattgaggc caagagagca gaaagagaag caagcaatcc
     1681 tcagttgaca caggtatctg cagatttcag aaatgccatg gatctctgga aggattaatc
     1741 catgcaagta ggcttgaaat ccgagtaaga tttgcaagct gtgtataatt gtgatagaat
     1801 aaatagaaaa taaattgaaa aaagaggtat gtgaaatgtc acgtaaacca tttatcgctg
     1861 gtaactggaa aatgaacaaa aatccagaag aagctaaagc attcgttgaa gcagttgcat
     1921 caaaacttcc ttcatcagat cttgttgaag caggtatcgc tgctccagct cttgatttga
     1981 caactgttct tgctgttgca aaaggctcga accttaaagt tgctgctcaa aactgctact
     2041 ttgaaaatgc aggtgctttc actggtgaaa ctagcccaca agttttgaaa gaaatcggta
     2101 ctgattacgt tgttatcggt cactcagaac gccgtgacta cttccatgaa actgacgaag
     2161 atatcaacaa aaaagcaaaa gcaatctttg cgaacggtat gcttccaatc atctgttgtg
     2221 gtgaatcact tgaaacttac gaagctggta aagctgctga attcgtaggt gctcaagtat
     2281 ctgctgcatt ggctggattg actgctgaac aagttgctgc ctcagttatc gcttatgagc
     2341 caatctgggc tatcggtact ggtaaatcag cttcacaaga cgatgcacaa aaaatgtgta
     2401 aagttgttcg tgacgttgta gctgctgact ttggtcaaga agtcgcagac aaagttcgtg
     2461 ttcaatacgg tggttctgtt aaacctgaaa atgttgcttc atacatggct tgcccagacg
     2521 ttgacggtgc ccttgtaggt ggtgcgtcac ttgaagctga aagcttcttg gctttgcttg
     2581 actttgtaaa ataatcagta agtagcaaaa gctaggtgga acagcattca gatgtctgtt
     2641 acatttttta taggagagga aagattgaaa ataaaaattg gattagcaag tatctgttta
     2701 ctaggcttgg caactagtca tgtcgctgca aatgaaactg aagtagcaaa aacttcgcag
     2761 gatacaacga cagcttcaag tagttcagag caaaatcagt cttctaataa aacgcaaacg
     2821 agcacagaag tacagactaa tgctgctgcc cactgggatg gggattatta tgtaaaggat
     2881 gatggctcta aagctcaaag tgaatggatt tttgacaact actataaggc ctggttttat
     2941 attaattcag atggtcgtta ctcgcagaat gaatggcatg gaaattacta cctgaaatca
     3001 ggtggatata tggcccaaaa cgagtggatc tatgacagta attacaagag ttggttttat
     3061 ctcaagtcag atggggctta tgctcatcaa gaatggcaat tgattggaaa taagtggtac
     3121 tacttcaaga agtggggtta catggctaaa agccaatggc aaggaagtta tttcttgaat
     3181 ggtcaaggag ctatgatgca aaatgaatgg ctctatgatc cagcctattc tgcttatttt
     3241 tatctaaaat ccgatggaac ttatgctaac caagagtggc aaaaagtggg cggcaaatgg
     3301 tactatttca agaagtgggg ctatatggct cggaatgagt ggcaaggcaa ctactatttg
     3361 actggaagtg gtgccatggc gactgacgaa gtgattatgg atggtactcg ctatatcttt
     3421 gcggcctctg gtgagctcaa agaaaagaaa gatttgaatg tcggctgggt tcacagagat
     3481 ggtaaacgct atttctttaa taatagagaa gaacaagtgg gaaccgaaca tgctaagaaa
     3541 atcattgata ttagtgagca caatggtcgt atcaatgatt ggaaaaaggt tattgatgag
     3601 aacgaggtgg atggtgtcat tgttcgtcta ggttatagcg gtaaagaaga caaggaattg
     3661 gcgcataaca ttaaggagtt aaaccgtctg ggaattcctt atggtgtcta tctctatacc
     3721 tatgctgaaa atgagaccga tgctgagaat gacgctaaac agaccattga acttataaag
     3781 aaatacaata tgaacctgtc ttaccctatc tattatgatg ttgagaactg ggaatatgta
     3841 aataagagca agagagctcc aagtgataca gacacttggg ttaaaatcat caacaagtac
     3901 atggacacga tgaagcaggc gggttatcaa aatgtgtatg tctatagcta tcgtagttta
     3961 ttacagacgc gtttaaaaca cccagatatt ttaaaacatg taaactgggt agcggcctat
     4021 acgaatgctt tagaatggga aaacccttat tattcaggag aaaaaggttg gcaatatacc
     4081 tcttctgaat acatgaaagg aatccaaggg cgcgtagatg tcagcgtttg gtattaagcg
     4141 atgatttgaa agagggatgt gatagtagca ccctcttttt ctttgtttta tgatagttca
     4201 tcctcgagta aattcaagat cttgctcgga aatgaagctt atatagtaga ttgaatatag
     4261 ataaagacct tgtgattggt aaaacatttt agaaattcat ttacctttcc taatcgactt
     4321 ggtttcatct tatttcaatc tattatagta ttggggaatt tcttcaaacc acatcagctt
     4381 ggtcagtcct acctgcgacc tcaaaacttg tgctttggtc aagctgggtt tagtttccta
     4441 gtttgctgat ggatttccat tgactataag catccaaccc tcttttttgt cttctaaaga
     4501 attcttaaat tatcagtcta ttgcaacttt tctcatataa gttctttgtc ttgctattgg
     4561 ttttccttag tagtatacta aggtagtaat cattaagaag tggttacaaa aaataatgaa
     4621 tgaggtaaag aaaatggtag aattgaaaaa agaagcagta aaagacgtaa catcattgac
     4681 aaaagcagcg ccagtagcat tggcaaaaac aaaggaagtc ttgaaccaag ctgttgctga
     4741 tttgtatgta gctcacgttg ctttgcacca agtgcactgg tatatgcatg gtcgtggttt
     4801 ccttgtatgg catccaaaaa tggatgagta catggaagct cttgacggtc aattggatga
     4861 aatcagcgaa cgcttgatta cactcggtgg aagcccattc tctacattga cagagttcct
     4921 tcaaaatagt gaaatcgaag aagaagctgg tgaataccgt aatgttgaag aaagcttgga
     4981 acgtgttctt gttatctacc gttacttgtc agaacttttc caaaaaggtt tggatgtcac
     5041 tgatgaagaa ggtgacgatg tgacaaacgg tatctttgca ggcgctaaaa ctgaaacaga
     5101 taaaacaatt tggatgcttg cagccgaact tggacaagca cctggtttgt aagaaataag
     5161 gaaacataga taaatctgta ggaaatttct tacagatttt ttctattttg taagctattc
     5221 caaaccagtc tttttttgag ttttttgata aaatagtact atcagtgaaa aggatggaag
     5281 catgactaag aaaatcgtag ctatttgggc ccaggatgaa gagggtgtga ttggtaagga
     5341 aaatcgtctg ccttggcatt tgccagcaga attgcagcac tttaaagaaa caactctgaa
     5401 tcatgctatc ttgatggggc gtgtgacctt tgatgggatg ggacgtcgct tgcttccaaa
     5461 acgggaaacc ttgattttga cgcgtaatcc ggaagaaaag atagatgggg ttgctacttt
     5521 tcaggacgtc cagtctgttc ttgactggta tcaggctcaa gaaaagaatc tctacattat
     5581 cggtgggaag caaatttttc aggcttttgg accttacctt gatgaagtga ttgtcactca
     5641 cattcatgct cgggtggaag gagataccta tttccctgaa gagcttgact tgtctctttt
     5701 tgagacagtt tcaagcaaat tttacgccaa agatgagaag aatccttatg attttaccat
     5761 ccaataccgc aagagaaagg aagtctaatg gaacggagtt tatttggtct attcacggct
     5821 tttctgtgtt tcatctgctt tctggcagga gcacaggctt ttcgtaaaaa gcgttacggg
     5881 ctttctatcc tcctttggtt aaatgccttt accaatctgg tcaatagtat tcatgctttt
     5941 tacatgacct tattttagat agaatgacag tatagaatgg aaggaaatca tgtctacaaa
     6001 tagaaaaaat gatatgatgg tttattgctc attttgtggc aaaaaccaag aagaagtaca
     6061 aaaaataatt gctggcaaca atgcttttat ttgtaatgaa tgcgtggagt tagctcagga
     6121 aatcattcga gaagaattgg ttgaggaagt cttggcagac ttgtctgagg tgccaaaacc
     6181 aattgaactc ctccatatct tgaaccacta tgtaattggt caagatcgtg ccaagcgtgc
     6241 cttggcagtg gcggtttata accactacaa acgcatcaat ttccacgata cacgcgaaga
     6301 gtcagaagat gtagatttgc agaagtcaaa cattttgatg attggcccaa ctggttcagg
     6361 gaaaactttc cttgcccaga ccttggctaa gagcttgaat gtaccttttg ctattgcgga
     6421 tgcgacagct ctgacggagg ctggttatgt gggtgaggac gtggaaaata tcctcctcaa
     6481 actcttgcag gttgctgact ttaacatcga acgtgcagag cgtggcatta tctatgtgga
     6541 tgaaattgac aagattgcca agaagagtga gaatgtgtct atcacacgtg atgtttctgg
     6601 tgaaggggtg caacaagccc ttctcaagat tattgaggga actgttgcta gcgtgccgcc
     6661 tcaaggtgga cgcaaacatc cacaacaaga gatgattcaa gtggatacaa aaaatatcct
     6721 cttcatcgtg ggtggtgctt ttgatggtat tgaagaaatt gtcaaacaac gtctgggtga
     6781 aaaagttatc ggatttggtc aaaacaataa ggcgattgac gaaaacagct catacatgca
     6841 agaaatcatc gctgaagaca ttcaaaaatt tggtattatc cctgagttga ttggacgctt
     6901 gcctgttttt gcggctcttg agcaattgac cgttgatgac ttggttcgca tcttgaaaga
     6961 gccaagaaat gccttggtga aacaatacca aaccttgctt tcttatgatg atgttgagtt
     7021 ggaatttgac gacgaagccc ttcaagagat tgctaataaa gcaatcgaac ggaagacagg
     7081 ggcgcgtgga cttcgctcca tcatcgaaga aaccatgcta gatgtcatgt ttgaggtgcc
     7141 gagtcaggaa aatgtgaaat tggttcgcat cactaaagaa actgtagatg gaacggataa
     7201 accgatccta gaaacagcct agaggtgact atggaactta atacacacaa tgctgaaatc
     7261 ttgctcagtg cagctaataa gtcccactat ccgcaggatg aactgccaga gattgcccta
     7321 gcagggcgtt caaatgttgg taaatccagc tttatcaaca ctatgttgaa ccgtaagaat
     7381 ctcgcccgta catcaggaaa acctggtaaa acccagctcc tgaacttttt taacattgat
     7441 gacaagatgc gctttgtgga tgtgcctggt tatggctatg ctcgtgtttc taaaaaggaa
     7501 cgtgaaaagt gggggtgcat gattgaggag tacttaacga ctcgggaaaa tctccgtgcg
     7561 gttgtcagtc tagttgacct tcgtcatgac ccgtcagcag atgatgtgca gatgtacgaa
     7621 tttctcaagt attatgagat tccagtcatc attgtggcga ccaaggcgga caagattcct
     7681 cgtggtaaat ggaacaagca tgaatcagca atcaaaaaga aattaaactt tgacccgagt
     7741 gacgatttca tcctcttttc atctgtcagt aaggcaggga tggatgaggc ttgggatgca
     7801 atcttagaaa aattgtgagg aaaagaaaat ggcaaaaaca attcatacag ataaggcccc
     7861 aaaggctatc ggaccctatg ttcaaggaaa aatcgttggc aaccttttgt ttgctagcgg
     7921 tcaagttccc ctatcccctg aaactgggga aattgtagga gagaatatcc aagaacagac
     7981 agagcaagtc ttgaaaaaca tcggtgctat tttggcagaa gcaggaacag actttgacca
     8041 tgttgtcaaa acaacttgct tcttgagcga tatgaacgac tttgttcctt ttaatgaggt
     8101 ttaccaaacg gccttcaaag aggaattccc agctcgttca gctgtggagg tagctcgtct
     8161 tcctcgtgat gtaaaagtcg aaattgaagt catcgcagag attggataag ctagttgaag
     8221 tttggtgttg ccaaacttct tttgatataa ggagaaaaag atgacaaaga aacaacttca
     8281 cttggtgatt gtgacaggga tgagtggcgc agggaaaact gtagccattc agtccttcga
     8341 ggatctaggt tatttcacca ttgataatat gccgccagct ctcttgccta agtttttgca
     8401 gctggttgaa attaaggaag acaatcctaa gttggccttg gtagtggata tgcgtagccg
     8461 ttctttcttt tcagagattc aagctgtttt ggatgagttg gaaaatcaag atggtttgga
     8521 tttcaaaatc ctctttttgg atgcggctga taaggaattg gtcgctcgtt acaaggagac
     8581 cagacggagt cacccactag cagcagacgg tcgtatttta gatggaatca agttggaacg
     8641 tgaactcttg gcacctttga aaaatatgag ccaaaatgtg gtggatacga ctgaactcac
     8701 tccacgtgag ctgcgcaaaa cccttgcaga gcagttttca gaccaagaac aagcccagtc
     8761 tttccgtatc gaagtcatgt ctttcggatt taagtatgga atcccgattg atgcggactt
     8821 ggtctttgat gtccgtttct tgccaaatcc ctattatcta ccagaactga gaaaccaaac
     8881 gggtgtggat gaacctgttt atgattatgt catgaaccat cctgagtcag aagactttta
     8941 tcaacattta ttggccttga ttgagccgat tctgccaagt taccaaaagg aaggtaagtc
     9001 cgttttgacc attgccatgg gatgtacggg tggacaacac cgtagtgtgg catttgctaa
     9061 acgcttggcg caggacttat ccaagaattg gtctgttaat gaagggcatc gcgacaaaga
     9121 ccgcagaaag gaaacggtaa accgttcatg agaaaaccaa agataacggt gattggtgga
     9181 gggactggaa gtcccgtcat tctaaaaagt ctgcgggaaa aagatgtgga aatcgcagct
     9241 atcgtgacgg tggcagatga tggtggttct tcaggtgaac tccgaaaaaa tatgcaacag
     9301 ttgacaccgc caggtgatct tcgtaatgtc cttgtggcca tgtcggatat gcctaagttt
     9361 tatgagaagg tctttcagta tcggttctct gaggatgccg gagcctttgc tggccatcca
     9421 ttgggaaatc tcatcattgc tggcttgtca gaaatgcagg gttcaaccta taatgccatg
     9481 cagttattga gcaaattttt ccatacaaca gggaaaattt atccttccag tgaccatcct
     9541 ttgacccttc atgcagtctt tcaggatggg acagaagtgg ctggagagag tcatattgta
     9601 gaccatcgag gcataattga caatgtctat gtgaccaatg ccctaaacga tgatacgcct
     9661 ctggccagcc gtcgagtagt gcagaccatc cttgaaagtg acatgattgt cctagggcca
     9721 ggttccctct ttacctctat tttgcccaat atcgtgatta aggaaattgg gcgggctctt
     9781 ttggaaacca aggcagaaat tgcctatgtc tgcaatatca tgacccaacg tggggagacg
     9841 gaacacttta cagatagcga ccacgtggaa gtcttgcatc gtcaccttgg tcgccctttt
     9901 atcgacactg tcttggtgaa tattgaaaaa gtgcctcagg aatacatgaa ttccaaccgt
     9961 tttgatgaat acttagtgca agtggaacac gattttgtag gtctttgtaa gcaagtttcg
    10021 cgcgtgattt catctaactt ccttcgtctg gaaaatggcg gtgccttcca cgatggagat
    10081 ttgattgtgg acgagttgat gcgcattata caggtgaaaa aatgagtttc acagtagcag
    10141 taaaagaaga aatcctgggc caacaccatc tgagctggca tgaattatct gccattatca
    10201 aaatgtctgg tagcatcggt ctctcgactt cgggcttgac tttgtcagtt gtgacagaaa
    10261 atgccaaact ggcccgtcac ctctatgagt cctttctcca tctctatgaa atcaaatctg
    10321 aaattcgtca ccaccaacgg agcaatcttc gcaagaatcg cgtctatacc gtttttacag
    10381 atgaaaaggt gcaggatttg ttaagtgatt tgcacttggc agactctttc tttggcctgg
    10441 aaacaggtat tgatgaggcg attttatcgg atgaggaagc aggtcgtgcc tatctctgtg
    10501 gcgctttctt ggcaaatggg agcattcgtg accctgagtc aggcaagtac cagttggaaa
    10561 tcagttctgt ttatctggac cacgcgcaag gaattgcctc ccttctccaa cagtttttac
    10621 tggatgccaa ggtgcttgag cgcaagaagg gggctgtgac ctatctccag cgagcagaag
    10681 acattatgga cttcttgata gtaataggag ccatgcaggc acgtgatgat tttgagcggg
    10741 ttaagatttt gcgagaaacc cgtaacgacc tcaatcgggc taataatgcc gagacagcta
    10801 atatcgctcg gacagtttct gccagcatga agaccattaa caatatcagt aaaatcaaag
    10861 atatcatggg cttagaaaat ttgccagtag atttgcagga agtggcgcaa ctgaggattc
    10921 agcacccaga ctactctatc cagcagttag cagatagtct cagcacccct ctgaccaaaa
    10981 gtggtgtcaa tcacagactc agaaaaatca ataagatagc ggatgaatta taaaaccacg
    11041 aatcctatgt gactcgtggt tcttttttat aaactagtag agtgttttgg ttgtactttt
    11101 tgttcagggt caatgtagtt gatagcgttg ttgacagcag ttggagcttc tccgaggcct
    11161 gtcgcaatca gatcaatttt tccgtcatag tagcagcagt caccgatagc atagatacct
    11221 gcttggctgg attcctgttt gctgttgacg ataatcttgt gacggttgag gtcgagcccc
    11281 cagtttttaa ggttaccgac agaagatttg aaaccatagt tgacaaagag gtggtctagg
    11341 tcaatggttt cagtttcatc agatttgact tttgtgattt caagtttatc aagtgttttt
    11401 ccatttccaa ggagttggct aggggcgaat ggtgtcttga tggttacaga tgattcttgc
    11461 aaggcttgaa cactgtgttc caaggcacgg aaattatctc tgcggtgaac aagggtagtt
    11521 ggtgcgattt tttcaaaagc caaagcccaa tccacagccg agtctccccc accaagaatc
    11581 gtcactttct taccagcgta ttgctgaatg ttagaaacgt ggtagtggat attttcatag
    11641 ccctcaaccc cttcaagttc cagcggacgt ggtttgaagg caccgccacc catagcgatg
    11701 ataactgttt tagtcaggtg acttccttta gaagttgtga tggcaaattc ttcttgtttg
    11761 tcaatctcaa gaaccgtttc attgagatga ataggggtat caaatccatt tagctgttca
    11821 atcaagcggt tagtcaactc ttctccagtc aggtttggga agcctggtac gtctaggatt
    11881 tccttttcag ggtagagaat agcaggttgt ccacctagct ggggaagaga gtcgatgatt
    11941 tgaaccttgg cttggcgtag gtgggcataa aaggctgcaa aaagtccgac aggaccacca
    12001 cccacaatgg taatatcata gagttgagac atagtttctc ctttgttttt tctagtcagt
    12061 ttattttatc atatttttgt tcaatgtaaa atggactagg atagggaaaa aaatgccaga
    12121 aaatggtata ataggacgaa agtgttttga tagggaggat atcagggatg aattttcaac
    12181 aattatccaa tctgcaatat tggaccagtt tgtttgcaag tccatggacg atagctatca
    12241 atctgattga tattttgatt gttgcttata ttttatacca ttttacaaaa gctattgcag
    12301 gaaccaagat tatgattttg gtacgtggag ttttggtatt tattttagct caaatccttg
    12361 caaatatgat tggtttgact acgatttctt ggttaatcaa tcaaattatt acttatgggg
    12421 ttattgcggc ggttgttatc ttctctccag agattcggac tggtttggaa cgtttgggaa
    12481 gagcgacaga tttcttttcc aatgccccta ttagtgctga ggaacagatg attcgtgcct
    12541 ttgttaagtc tgttgaatac atgagtcctc gtaaaatcgg ggccttggtt gctattcagc
    12601 gtgtacgtac cttgcaggag tatatttcga caggaattcc cttggatgct aagatttctg
    12661 cagaacttct cattaacatt tttattccca acactcccct acatgatggt gcggtgatta
    12721 tcaaagaaga acgtatcgct gtgacgtctg cctatctgcc cttgacaaaa aacacaggga
    12781 tttccaagga atttgggacc agacaccggg cggctatcgg tttatcagaa gtctcagatg
    12841 ccttgacttt tgtcgtatca gaggaaacgg gaggaatttc gataacctat aatggaaggt
    12901 ttaagcacaa cctaacactt gatgaatttg aaacagaatt acgtgaaatc ttacttccaa
    12961 aagaggaagt gggtcttagt tttaaagaac gattgctagg aggatggaaa catgaaaaaa
    13021 aatagtttat atatcatatc ctcactcttt tttgcttgtg tcttatttgt ctatgctacg
    13081 gcgacgaatt ttcaaaacag taccagtgct aggcaggtaa aaacggaaac ctatactaat
    13141 acagtaacaa atgtccctat tgacatacgc tataatagtg ataagtattt tattagcggt
    13201 tttgcttcag aagtatcagt ggtcttgact ggtgcaaatc gcctatcgct agctagtgaa
    13261 atgcaagaaa gtacacgtaa attcaaggtt actgctgacc taacagatgc cggtgttgga
    13321 acgattgaag ttcctttgag cattgaagat ttacccaatg ggctgaccgc tgtggcgact
    13381 ccgcaaaaaa ttacagtcaa gattggtaag aaggctcaga aggataaggt aaagattgta
    13441 ccagagattg accctagtca aattgatagt cgggtacaaa ttgaaaatgt catggtgtca
    13501 gataaagaag tgtctattac gagtgaccaa gagacattgg atagaattga taagattatc
    13561 gctgtcttgc caactagcga acgcataaca ggtaattaca gtggttcagt acctttgcag
    13621 gcaatcgacc gcaatggtgt tgtcttaccg gcagttatca ctccgtttga tacaataatg
    13681 aaggtgacta caaaaccagt agcaccaagt tcaagcacat caaattcaag tacaagcagt
    13741 tcatcggaga catcttcgtc aacgaaagca actagttcaa aaacgaatta aaaatagaaa
    13801 aggattttat aacaatgggt aaatattttg ggactgatgg agtccgtgga gaagctaacc
    13861 tagaactaac accagaatta gcctttaaac taggacgttt tggaggctat gttcttagtc
    13921 aacatgaaac ggaagcgccg aaagtctttg taggacgtga cacacgtatt tcaggggaaa
    13981 tgctggaatc ggccttggtg gcaggtctcc tttcagtagg gattcacgta tacaaacttg
    14041 gtgtccttgc aacaccagca gtagcttact tggttgaaac tgaaggagca agtgccggtg
    14101 tcatgatttc tgctagccac aacccagccc ttgataacgg aatcaagttc tttggcggtg
    14161 atggcttcaa actagatgat gaaaaagaag cagaaattga agccttgcta gatgctgagg
    14221 aagacactct tcctcgtcca agtgcagaag gcttaggaat tttggtagat tatccagaag
    14281 gcttgcgtaa gtatgaagga taccttgttt caactggaac tcctcttgat ggaatgaagg
    14341 ttgccttgga tacagctaat ggagcagctt ctaccagtgc ccgtcaaatc tttgcagacc
    14401 ttggtgccca attgacggtt atcggggaaa caccagacgg tcttaacatc aaccttaatg
    14461 ttggttcaac acatccagaa gcccttcaag aagtggtcaa agaaagtggg tcagctattg
    14521 gtttggcctt tgatggagac agtgaccgct tgattgctgt tgatgagaat ggtgacatcg
    14581 tcgatggtga caagattatg tacatcatcg gaaaatacct ttctgaaaaa ggacaattgg
    14641 ctcaaaatac aattgtgaca actgttatgt ctaaccttgg tttccacaag gccttgaatc
    14701 gcgaaggtat taacaaggca gttactgcag ttggtgaccg ctacgttgtt gaagaaatga
    14761 gaaaatcagg ctacaacctt ggtggtgaac agtctggtca cgttatcttg atggattaca
    14821 ataccacagg tgatggtcaa ttatcagcag ttcaattgac taaaatcatg aaggaaactg
    14881 gtaagagctt atcagagttg gcggcagaag taacgattta tccacaaaaa ttagttaata
    14941 tccgagtgga aaacgtcatg aaggaaaagg ccatggaagt gccagctatc aaggccatca
    15001 tcgagaagat ggaagaagaa atggcgggga acggccgtat ccttgttcgt ccaagtggaa
    15061 cagagcccct cttgcgtgtt atggcagaag cgcctacaac agaagaagtg gactactatg
    15121 ttgataccat cacagatgta gttcgtgctg aaattgggat tgactaaatc ctagaaaact
    15181 agatgaaaat aaaaaattat ggtacaatag cttataatat tgtagccgat ggagaaagac
    15241 atgaatctta aacgtgaaca agaatttgtt agtcagtatc attttgatgc gcgtaatttt
    15301 gaatgggaaa atgaaaatgg agctcctgaa accaaggtgg atgtgaattt ccaattgctc
    15361 caacatgacc aagaaaatca agtgacttcc ttgattgtca tcttgagctt tatgattgta
    15421 tttgataagt ttgtcattag tggaacaatc tcacaagtaa accatatcga tggccgtatt
    15481 gtcaatgaac caagtgaatt gaaccaagaa gaggtggaaa ctttggctcg tccatgtttg
    15541 aacatgctca atcgtttgac ttacgaagtg actgaaattg cattagactt accaggaatt
    15601 aatttggagt tctaatatga aattagctgt tttcacagat tcctctgctt atctcagtgc
    15661 agagaccttg caaagagaag acttgtttgt cttggatatt cctgtcaata ttgatggtga
    15721 ggaatatgtc gaaggcatca atctgtctgc tgaggaattt taccaaaaaa tggctcaggc
    15781 ttctgaattg cctaagacca gtcaaccaag tattgccaag ttagatgaaa tcttaacttc
    15841 gctcaaagaa caaggctata cacatgcctt ggggcttttc ctatcttctg gaatttcagg
    15901 tttttaccaa aatatccagt atatggtcga tgactatgag ggcttaacca ttgctttccc
    15961 agacactttg attacaagtg ctcccctagg catcatggtt gaaagcgtct ttaattggcg
    16021 tgatcagggc gatgattttg ccagcattca ggataagcta gccattcaaa tcagccgtac
    16081 gtcagccttt atcatggtag atgacttgga tcatttggta aaaggtggac gcctttcaaa
    16141 tggggctgcc attttgggca atttgcttag cattaagcca atcctttatt ttaacgatca
    16201 aggtgtgatt gaagtttacg aaaaagttcg tactgaaaag aaggccacca aacgcttaat
    16261 tgaaatcatc aaggaaacaa cggcttcagg tcaataccgg gtcattgtca ttcatgggaa
    16321 tgctcctgaa aaggctgaag aattgcgtca gcatttgctt gattttggct tgggttcgga
    16381 tgtttcactt gctacatttg gtagtgtcat tggaacgcac ctaggagcag gaagtattgc
    16441 tctgggttat attccagtga tttagtaggc acttctaggc tagttaatct tagcaattga
    16501 acacggacta cctgcctctt gaaaaaagat gtctgtcttg cctttcgtgg aaagtcagtg
    16561 ccattcccta tttttcatgg gtggctaaga tcctttgtat cttgataatt gaacacgcct
    16621 ggaaccctgt gtgaaaaaga tagttcttcc aaggagtaga cacttcttga tcagaattcc
    16681 tattttcact ttgtgttctt acaggctttg tatcttagac aggagtagag atgagtattc
    16741 gagtaattat tgccggtttt aagggaaaga tgggtcaagc tgcttgccag atggtcttga
    16801 ctgattcaga tttggacttg gtagcagttt tggatccttt tgagtctgag tcagaatggc
    16861 agggtattcc tgttttcaag gataaggctg atttagctgg ttttgaagcg gatgtctggg
    16921 tagactttac tactccagct gttgcctacg aaaatacacg ttttgctctt gaaaatggct
    16981 ttgctccagt agttggaacg actggtttca cgagtgaaga aattgcagag ctaaaagaat
    17041 tttctcgtgc ccaagacttg ggtggcctga ttgcccctaa ctttgccttg ggtgctgtct
    17101 tactcatgca atttgcgacg caggctgcca aatatttccc aaatgtggag attattgagc
    17161 tccatcatga caagaaaaag gatgctccga gtggaacagc cattaaaaca gctgagttga
    17221 tggcagaggt tcgagagtca attcagcaag gtgcagcaga tgaggaagag ctgattgctg
    17281 gtgctcgtgg tgctgacttt gatggtatgc gcatccactc agttcgtttg ccaggtttgg
    17341 tagcccatca ggaagtcatc tttggcaatc aaggagaagg attgaccctc cgtcatgact
    17401 cctatgatcg catctccttc atgacaggag tcaatttggg aattaaagaa gttgtcaagc
    17461 gtcatgagct tgtctatgga ttagaacact tattatgaga ttaacgcaaa tgccttctga
    17521 atttcagaag gctttaccag tattagaaaa aattaaagaa gcaggctttg aggcttattt
    17581 tgttgggggc tctgttcgag atgcccttct ccatagccct atccacgatg tggatattgc
    17641 gacgtcttct tatccagaag agaccaagca gatttttccg cgaacagccg atatcggaat
    17701 cgagcatgga accgtcttgg tcttagatgg ggatgaggag tatgaggtaa caacctttcg
    17761 gacagaggat gtctatgtgg actatcgcag acccagtgcg gtttcctttg tgcgatcgct
    17821 agaagaagac ctcaaacgcc gtgatttcac agtcaacgcc tttgccttgg atgagacagg
    17881 agaaatcgtt gacttgttcc atggtttaga agatttggaa aagcaagtct tgcgagcagt
    17941 tggagtggct agtgagcgtt tcaacgaaga tgctttacgg attatgcgtg gtttccgttt
    18001 tcaggctagt cttggttttg cacttgagcc agaaacattt aaagctatga agaccttgac
    18061 gccgcttttg gagaaaattt ctgtagagcg taccttcgtc gagtttgata aactcttgct
    18121 ggctccattt tggagaaggg gcttggcttc catgattgag agtcaagctt atgactatct
    18181 ccctgatatg gcatctagcc aggacaagct caacagactg tttgatttgg agactgattt
    18241 tacttttgaa tcctctgaac aagcctgggc ggctttacta tgggctttgg agattgaaaa
    18301 tgcgcagtca tttttgaaat cttggaagac ctcacgccag tttgccaagc aagttcagga
    18361 tttgctgatt attttggctc tgcgtgaaaa tggagaattg agcaagcgag attgttatcg
    18421 ctttgacata gatttgcttt tacaggctga aaatcttcgt caagctcaag gaaaagaagt
    18481 caacccacaa gccatcacag aaaaatacca aagcttgact atccatgaca agaaagagat
    18541 tcagattaat ggcggtattt tgatcaagga atatggctat cagccaggcc cagacttggg
    18601 agagatttta acagagattg agtttgccat tgtcgatgga gaattggaga ataatcgtga
    18661 agccatccat gcttacctga gggagaaaaa atgagtgatt ttatcgttga aaaactaagt
    18721 aaatccgttg gtgacaagac cgtttttagg gatatttcct ttattatcca tgacttagac
    18781 agaattggtt taatcggtgt caatggaact ggcaagacca cccttttgga cgtcctttct
    18841 ggtgtttctg gatttgatgg ggatgtcagt cctttttcag ctaaaaatga ttaccagatt
    18901 ggttacttga ctcaggatcc tgattttgat gatagaaaga cagttttgga tacggttcta
    18961 tctagtgaac tcaaggaaat ccagctcatt cgtgagtatg aattgattat gctcgactat
    19021 agtgaggaca agcaggcgcg tttggaacgt gtcatggcag agatggactc tctccaagct
    19081 tgggaaatcg agagtcaggt caagaccgtt cttagcaaat tgggcattca agacttatct
    19141 actcctgttg gggaattgtc aggtggtctg agaagacggg tacagttggc acaagtctta
    19201 cttggcaacc acgacctctt gcttttggat gagccgacca accatctgga tattgcgatt
    19261 attgagtggc tgaccctctt tttgaaaaat tctaagaaga ccgtcctttt tatcactcac
    19321 gatcgttatt tcttagacgc tttgtcaaca cggattttcg agttggagcg agcaggcttg
    19381 acagaatatc agggcaatta ccaggactat gttcgcctta aggcagaaca ggacgagcgc
    19441 gatgcagctc tacttcataa aaaagaacaa ctctacaaac aagaactggc ctggatgcgc
    19501 agacaaccgc aggcgcgtgc gaccaagcag caggctcgta tcaatcgttt ccacaacttg
    19561 aagaaagaac tttcaggtgg cgttgctgag acagacttaa ctatgaactt tgaaaccagt
    19621 cggattggga agaaagtcat cgagtttcag gatgtttcct ttgcctatga aaacaagccc
    19681 attttgcaag attttaatct cttggtacag gtcaaagatc gtatcggaat tgttggggac
    19741 aacggtgttg gaaaatcaac cttactcaac ttaattgcag gaagtcttga gtcaactaaa
    19801 ggacaagttg tgattgggga aactgttcgc atcgcctatt tctctcagca aattgagggc
    19861 ttagatgaaa gcaagagagt tatcaattac ctgcaggaag tggcagaaga ggtcaagacc
    19921 agtggcggtt ctacgacttc gattgcagag ttgctggagc agttcctttt cccacgttcg
    19981 acgcatggaa ccttgattga gaaattgtca gggggtgaga aaaaacgtct ttatctcctc
    20041 aaactgcttt tggaaaaacc aaatgttctt cttttagacg agccaaccaa tgacctagat
    20101 attgcaactt tgacagtctt agagaatttc ttgcaaggtt ttgcaggtcc cgttttaaca
    20161 gtcagtcacg accgctattt cttggataag gtagcgacca agattctcgc ttttgaggat
    20221 ggcaagattc gtcctttctt tggtcattac accgactatc ttgatgaaaa aacttttgaa
    20281 acagatatgg ccaatcaagt gcaaaaggcc gaaaaggaaa aagtggtcaa ggttcgagaa
    20341 gacaagaaac gcatgaccta ccaagaaaag caggagtggg caagtattga aggtgatatt
    20401 gaagccttgg aaaaacgtat cgctgctatt gaagaggaaa tgcaggctaa cggctctgac
    20461 tttggtaagc tggctactct ccaaaaagaa ttggatgaga aaaatgaagc actccttgaa
    20521 aaatacgaac gctatgagta tctcagtgaa tttgatagtt aatactattc gaaaatcaaa
    20581 gtgcaaacta tgtcggcaat gtgaagccga catagtttgc actttgattt ttattgagta
    20641 tgaaagaata gaaaaatccc gtctcatgaa aggatttctc tattcttttt tggtttcttg
    20701 atgaaagata ttttgccagg tttcgtggcg gcgccagata ctggtatgga tgataccatc
    20761 taactcatag cagatgagtt tggttttttg actgatggaa gtaatttgaa tgtccttgat
    20821 agcagaattt aactcttttt tggtcttata agcctcttta tccatctgct ctccatcttg
    20881 gcgaatatag acaaaatcat cagtcaagag ttcttctagt tggtttcctt ggtcaatcaa
    20941 ttgttcacgc ataagcaatt ttttatagac attgtctaaa atttcatcct cagggatagg
    21001 tgctggttcg atatggacat cggtatcaaa gacgccaaaa cgattctcca gcatagactc
    21061 gacctgatcc gcgatttcat ggctttcaaa aacagacaag tcaggattca tctctagtgt
    21121 aatatccagg tagatgttgc taccgtaggt gcgacctctt tgcgatttga ccttgctgat
    21181 tttgggaatt tccatgatag ccttttggta gtcctcgagc aggcggtcgt caaagccatc
    21241 tgaaagacta aaggaagact cgatgaagat attataggca gtcttcaaga taaagaaagt
    21301 gatgatgata gcaaccagtt tatccacaat cggataattg aaactactag ctaggatggc
    21361 aatggcggtt ccaagtgagg taacagcgtc agaaagattg tccttagcag ctgccttcag
    21421 cgccttggag ttggatttct tactgaggcg agtattgtag agatagacca caaacataat
    21481 cgctgcagaa atgattccta gagttgcacc aagaggatca atgaccgttt cttcccgact
    21541 gagaatcttt tgaatggtat ctcttagaac atcgaaaccg acatagaaca tgatgataga
    21601 agtgatcaag cttgccaaat cttcaatctt ccaatgacca aaacggtggt cacggtctgc
    21661 aggctggcgc gccatccgaa tcccgattaa gagggccaca tttccaatga tgtccgatac
    21721 gttattaaaa ccatcggcca ccaaactgga tgaatgaagg agatgaccag ctgctaattt
    21781 ggctgcagac aagatcaaat aggtcgaaat actgataata gctccacgct cagctaactt
    21841 gagatttgag atagattgct tcattcaact tcctttctag tcacatagca ttctaccatt
    21901 atactggttt tcaaggaaaa attcaaataa ggcagttttt actctttgaa aactccccaa
    21961 aatttggtat aatggtacta ctcaagggag tagctggcag aaacctgtga tagtgtcgtc
    22021 attccgaatt ttatgctgaa aagtatgctt tccggcccta tcttaaacag cgagacttgt
    22081 tatgattaac aggtctcttt ttgtttgtcg tgaaaagata tgacagaaag agagtctgtt
    22141 ttgcacacaa tgtcaaggag gagacacatg tcaaaagaac aaaaacgcca agcgttttat
    22201 actcagagcc ctgaagaggt cttgcaggct gtggatgcga ccgagcaagg tttgtcatca
    22261 agtgaggcgg aaaagcgcct tgccgaattt gggcacaatg aactcgaaga aggcgagaaa
    22321 cgatcaatct tggtcaaatt catcgagcaa tttaaggatt tgatgattat catcctagtt
    22381 gcggcagcaa tcttgtcagt cgtgacttct ggtggggaag atatcgcaga tgccattatc
    22441 atcctagctg tggttatcat caacgctgcc tttggtgttt accaagaagg aaaagctgaa
    22501 gaagctatcg aagccctcaa atccatgtct agtccagttg cccgcgttct tcgtgatgga
    22561 cacatggcag agattgactc taaagaattg gtaccaggcg atatcgttgc ccttgaagca
    22621 ggtgacgtgg taccagcgga cctacgtttg atagaagcca actctcttaa aattgaagaa
    22681 gcagccttga caggtgaatc tgtaccagtc gaaaaagact tgtcagtcga gcttgcgaca
    22741 gatgctggta ttggtgaccg tgtcaacatg gccttccaaa actcaaacgt aacctatggt
    22801 cgtgggatgg gtgttgttgt caatacaggt atgtacactg aagttggtca tattgctggt
    22861 atgcttcaag atgcggatga gactgataca ccactcaaac aaaatttgaa caacctttct
    22921 aaggtcttga cctatgctat cttggtcatt gcccttgtta cttttgtagt gggtgtcttc
    22981 attcaaggga aaaatccact tggtgagttg ttgacttctg ttgcccttgc cgttgcagcc
    23041 attccagaag gacttcctgc tatcgttacc atcgttcttt ctcttggtac tcaagttttg
    23101 gccaaacgac attccatcgt tcgtaagttg ccagcagttg aaacacttgg ttcaactgaa
    23161 atcatcgctt ctgataagac tggtacgctg actatgaaca agatgacagt cgaaaaagtc
    23221 ttctacgatg cggttctaca tgactcagct gatgatattg aactaggtct tgaaatgcca
    23281 ctacttcgtt cagttgtctt ggccaatgat acgaaaatcg atgtggaagg taacttgatt
    23341 ggtgacccaa ccgaaacagc ctttatccaa tatgccttgg acaagggcta tgatgtcaaa
    23401 ggtttcttag agaaatatcc tcgtgtagct gaattgccat ttgactctga ccgtaagctc
    23461 atgtcaacag ttcacccatt gccagatggt cgtttccttg tagcagtcaa gggtgcgcca
    23521 gaccaactct taaaacgttg tcttcttcgt gataaggctg gggatattgc tccgattgat
    23581 gagaaggtta caaatctcat tcatacaaac aattctgaaa tggctcatca agccttgcgt
    23641 gtccttgcag gtgcttataa gattatcgat agtattccag aaaatctcac ttctgaagag
    23701 cttgaaaatg atttaatttt tactggtttg attgggatga ttgaccctga acgtcctgaa
    23761 gccgctgagg ctgttcgtgt ggctaaggaa gcgggaatcc gtccaattat gattacaggt
    23821 gaccaccaag acacagcgga agccattgcc aaacgtttgg gaatcattga cgcaaacgat
    23881 acagaaggtc acgttttaac tggtgctgaa ctcaatgaac tgtcagatga agaatttgaa
    23941 aaagtcgttg gtcaatactc tgtttatgcc cgtgtgtctc cagaacacaa ggttcgtatc
    24001 gtcaaggctt ggcaaaaaca aggtaaagtc gttgccatga caggtgacgg tgtcaatgac
    24061 gcgccagctc tgaaaacagc cgatatcggt atcggtatgg gaatcactgg tacagaggtt
    24121 tctaaggggg cttctgatat gattcttgca gatgataact ttgcgactat tatcgtcgca
    24181 gtggaagaag gacgtaaggt cttctcaaac attcaaaaga ctattcagta cctactttct
    24241 gctaatactg ctgaagtatt aaccatcttc ctatcaacct tgtttggttg ggatgtctta
    24301 cagccggttc atcttttgtg gatcaacttg gtaacggata ccttcccagc tatcgctctt
    24361 ggtgttgaac ctgcggaacc tggtgtcatg aatcataaac cacgtggacg caaggcaagc
    24421 ttcttctcag gtggtgtttt gagttctatc atttatcaag gtgtactcca agcagctctt
    24481 gttatgagtg tttatggcct tgcgattgct tacccagttc atgtgggtga caatcatgct
    24541 attcatgcag atgccctaac gatggccttt gcaacccttg gtttgattca gctcttccat
    24601 gcctacaatg tcaagtctgt ctaccaatcc atcttgacag ttggcccatt caagtctaag
    24661 acctttaact ggtccatctt ggtatccttt atccttctca tggcaacaat cgttgtagaa
    24721 ccgcttgaag gaatcttcca cgtaaccaaa ctagacttgt cacaatgggg aattgttatg
    24781 gctggaagct tctcaatgat tatcatcgtc gaaatcgtta agtttactca acgcaaactc
    24841 ggttttgaca agaatgcgat ttaagggagg tttcggatgt cagcatatca attaccgacc
    24901 gtatggcagg atgaagctag taatcaagga gcttttacgg ggctaaacag accaacagca
    24961 ggtgcccgtt tcgaacaaaa cttgccaaaa ggagaacaag cttttcagct ttattcactg
    25021 ggaacaccaa atggtgtgaa ggttactatc ttattggaag aattactaga agctggtttt
    25081 aaggaagcgg cttacgactt gtataagatt gctatcatgg atggggatca attcggctca
    25141 gactttgtga agctcaatcc aaattccaag attccagcct tattggacca gtcaggtact
    25201 gaaaacgtaa gagtctttga gtctgctcat attcttcttt accttgctga gaaatttgga
    25261 gcctttttac caagtaatcc tgtggaaaag gtagaagttt tgaattggct attctggcaa
    25321 gtaggtgcag caccttttct aggtggggga tttggacatt tcttcaatta tgctcctgaa
    25381 aaattggaat atcctattaa ccgttttacg atggaagtga aacgccagtt ggatttattg
    25441 gataaggaat tggctcagaa accttatatt gcaggcaatg actatacgat tgcagatatt
    25501 gctatctggt cttggtatgg acagttagtt caaggaaatc tttaccaagg ttctgcaaaa
    25561 ttcttggatg cctcaagtta tcaaaatcta gtaaaatggg cagaaaaaat tgccaatcgt
    25621 ccagctgtta agcgtggctt ggaagtaact tatacagaaa ttaaatagca aaaaagtcat
    25681 cttcggatgg ctttttgcta cagttgaggg aaagggtttg cagttatcgg cttttgtgct
    25741 ataattgcta taatatagta aaaagcaaga ggagtgtgag gaagtggaaa agaaaattgt
    25801 gaaggatatc ttatttttat ctcaagtgtc tcagccggca agtcaggagg acctttatct
    25861 tgccagagat ttgcaggata cactcttagc aaatcgtgat acctgtgttg gtctagctgc
    25921 caatatgatt ggggtgcaga agcgcgtgat tatctttaat cttggcttgg ttcccgtggt
    25981 catgtttaac ccagtgcttc tgtcctttga aggatcctat gaggcagaag aaggctgttt
    26041 gtccttggta ggtgtgagat caactaagcg ttatgaaacc ataaggcttg cctatcgtga
    26101 cagcaagtgg caggaacaga ccattacctt gacaggcttc ccagctcaga tttgccagca
    26161 tgagttggat cacttggaag gacgaatcat ttaggaggaa agcaaatgaa acgaatagtc
    26221 tttgaactta tttttatcgc aacgacctgg tatatctttt taccgcccct taacctgacc
    26281 agctgggaat ttcttttctt cctctgtggg catttgttag ttgtggcaat attatttggc
    26341 tttggcaagg ggataaacct tgtcaaaacg gttcatgtgc gccacggtaa ggcggaagct
    26401 gccttaaatc ttgagggttt caaaatcaat cggttaggga aaattctgtt agcttcgatt
    26461 ggaggaattc ttctcttggc agctttggtt tccttggtaa cttccagcat gtttcaggct
    26521 aaaaattatg ccaatgtagt cacggttacg gaaaaagact ttactgaatt tcctaagagt
    26581 gacaccagta aggttcctat cctagataga agtactgctg aaaaaattgg agaccgctac
    26641 ttgggttccc taaccgataa ggtgtcgcaa tacgtagcgg cagataccta tacccaattg
    26701 acaattgatg ggaaacctta tcgggtcaca ccactagaat atgcagaccc tatcaaatgg
    26761 tttaacaatc aagccaaggg aatcggtgag tatatcaagg tggacatggt aactggaaat
    26821 gcggatttgg tggacttgaa gacaccaatc aagtattcag actcggagta ttttaaccgt
    26881 gatgtcaaac gtcacctgcg cttgaagtac ccgaccaaaa tctttaaaac tccatctttt
    26941 gaggtggacg atgagggcaa tcctttctat gtagcaacgg tttaccaaaa gcaatttgga
    27001 cttgctgttc ctcgtcctgc ttcagtcatt atcttggatg ctacaaatgg agaaaccaag
    27061 gaatacagct tatcagatgt tccagaatgg gtggacagga tctatccagc agaggaaacc
    27121 attgagcaaa tcaactacaa cggcaagtac aaggacggtt tcttgaatgc catgatttcc
    27181 aagaaaaacg tgacccagac taccaatggc tataattact tgtctatcgg taatgacatc
    27241 tatctctaca caggtgtgac gtcggctaat gcggatgaga gtaatcttgg tttcatcctt
    27301 gaaaatatgc gaacaggaga aattactaag tatagcttgg cttctgcgac agaagaatca
    27361 gcccgtgaat cagcagaagg tgctgttcag gagaaatcct acaaagcaac cttcccaatc
    27421 ctcatcaacc tcaatgacaa gcctctctac atcatgggct taaaggacaa tgctggcttg
    27481 gtcaaagagt acgccctggt agacgcagtc gagtaccaaa atgttatcgt tgctactaca
    27541 gtggaagaga tgctcagcaa gtatgccaat aaaaacgacc ttgaaattga caatgcaacg
    27601 acagaaagca tcaagggagt agtagcagac ctcaaatcag ctgttatcaa gggagacact
    27661 gtctacttct ttaaagttga tggcaagata tacaaggtaa aggcttcagt atccgatgac
    27721 cttccttacc ttgaaaatgg taaaaccttc gaaggtcaag taggaaaaga caattatctc
    27781 aagaccttta aagtacaata atactcttcg aaaatctcta gctctatctg caacctcaaa
    27841 acagtgtttt gagcagcctg cggctagttt cctagtttgc tctt
//
LOCUS       DAVMEF010000006        26413 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMEF010000006 DAVMEF010000000
VERSION     DAVMEF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.006"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            12..1295
                     /locus_tag="VYQ30_000176"
     CDS             12..1295
                     /locus_tag="VYQ30_000176"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1965350.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(1343..3709)
                     /locus_tag="VYQ30_000177"
     CDS             complement(1343..3709)
                     /locus_tag="VYQ30_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1965351.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGITGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIQQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(3837..4451)
                     /gene="lepB"
                     /locus_tag="VYQ30_000178"
     CDS             complement(3837..4451)
                     /gene="lepB"
                     /locus_tag="VYQ30_000178"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1965352.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            complement(4455..5336)
                     /locus_tag="VYQ30_000179"
     CDS             complement(4455..5336)
                     /locus_tag="VYQ30_000179"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1965353.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5423..5725
                     /gene="zapA"
                     /locus_tag="VYQ30_000180"
     CDS             5423..5725
                     /gene="zapA"
                     /locus_tag="VYQ30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1965354.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5722..6270
                     /locus_tag="VYQ30_000181"
     CDS             5722..6270
                     /locus_tag="VYQ30_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1965355.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            6374..8710
                     /locus_tag="VYQ30_000182"
     CDS             6374..8710
                     /locus_tag="VYQ30_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1965356.1"
                     /translation="MNKKILETLEFDKVKALFEPNLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      8874..8962
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9053..10375
                     /locus_tag="VYQ30_000183"
     CDS             9053..10375
                     /locus_tag="VYQ30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1965357.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            10593..11141
                     /locus_tag="VYQ30_000184"
     CDS             10593..11141
                     /locus_tag="VYQ30_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1965358.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            11251..12153
                     /locus_tag="VYQ30_000185"
     CDS             11251..12153
                     /locus_tag="VYQ30_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1965359.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKRSS"
     gene            complement(12174..13448)
                     /gene="serS"
                     /locus_tag="VYQ30_000186"
     CDS             complement(12174..13448)
                     /gene="serS"
                     /locus_tag="VYQ30_000186"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1965360.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(13662..14033)
                     /locus_tag="VYQ30_000187"
     CDS             complement(13662..14033)
                     /locus_tag="VYQ30_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1965361.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKVSRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(14036..15400)
                     /locus_tag="VYQ30_000188"
     CDS             complement(14036..15400)
                     /locus_tag="VYQ30_000188"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1965362.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            15725..16510
                     /locus_tag="VYQ30_000189"
     CDS             15725..16510
                     /locus_tag="VYQ30_000189"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1965363.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            16604..17038
                     /locus_tag="VYQ30_000190"
     CDS             16604..17038
                     /locus_tag="VYQ30_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1965364.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            17038..18012
                     /locus_tag="VYQ30_000191"
     CDS             17038..18012
                     /locus_tag="VYQ30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1965365.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIHLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            18074..18298
                     /locus_tag="VYQ30_000192"
     CDS             18074..18298
                     /locus_tag="VYQ30_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1965366.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            18417..19391
                     /gene="fabK"
                     /locus_tag="VYQ30_000193"
     CDS             18417..19391
                     /gene="fabK"
                     /locus_tag="VYQ30_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1965367.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            19384..20304
                     /gene="fabD"
                     /locus_tag="VYQ30_000194"
     CDS             19384..20304
                     /gene="fabD"
                     /locus_tag="VYQ30_000194"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1965368.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            20338..21069
                     /gene="fabG"
                     /locus_tag="VYQ30_000195"
     CDS             20338..21069
                     /gene="fabG"
                     /locus_tag="VYQ30_000195"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1965369.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            21091..22326
                     /gene="fabF"
                     /locus_tag="VYQ30_000196"
     CDS             21091..22326
                     /gene="fabF"
                     /locus_tag="VYQ30_000196"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1965370.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGEIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            22329..22814
                     /locus_tag="VYQ30_000197"
     CDS             22329..22814
                     /locus_tag="VYQ30_000197"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1965371.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            22811..23233
                     /gene="fabZ"
                     /locus_tag="VYQ30_000198"
     CDS             22811..23233
                     /gene="fabZ"
                     /locus_tag="VYQ30_000198"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1965372.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            23245..24612
                     /gene="accC"
                     /locus_tag="VYQ30_000199"
     CDS             23245..24612
                     /gene="accC"
                     /locus_tag="VYQ30_000199"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1965373.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            24649..25515
                     /locus_tag="VYQ30_000200"
     CDS             24649..25515
                     /locus_tag="VYQ30_000200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1965374.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            25512..26279
                     /locus_tag="VYQ30_000201"
     CDS             25512..26279
                     /locus_tag="VYQ30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1965375.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
BASE COUNT         7528 a         5167 c         6131 g         7587 t
ORIGIN      
        1 gaaatgacag aatgtctgta tcatttgaaa acaaagaaac aaaccgtggt gtcttgactt
       61 tcactatctc tcaagaccaa atcaaaccag aattggaccg tgtcttcaag tcagtgaaga
      121 aatctcttaa tgttccaggt ttccgtaaag gtcaccttcc acgtcctatc ttcgataaaa
      181 aatttggtga agaatcactt taccaagacg ttatgaacgc tcttttgcca aacgcttatg
      241 aagcagctgt aaaagaagct ggtcttgaag tggttgccca accaaaaatt gacgtaactt
      301 caatggaaaa aggtcaagac tgggttatcg ctgctgaagt cgttacaaaa cctgaagtaa
      361 aattgggtga ctacaaaaac cttgaagtat cagttgatgt agaaaaagaa gtaactgacg
      421 ctgatgttga agagcgtatc gaacgcgaac gcaacaacct ggctgaattg gttatcaagg
      481 aagctgctgc tgaaaacggc gacactgttg tgatcgactt cgttggttct atcgacggtg
      541 ttgaatttga cggtggaaaa ggtgaaaact tctcacttgg acttggttca ggtcaattca
      601 tccctggttt cgaagaccaa ttggtaggtc actcagctgg cgaaaccgtt gatgttatcg
      661 taacattccc agaagactac caagcagaag accttgcagg taaagaagct aaattcgtga
      721 caactatcca cgaagtaaaa gctaaagaag ttccagctct tgacgatgaa cttgcaaaag
      781 acattgatga agaagttgaa acacttgctg acttgaaaga aaaataccgc aaagaattgg
      841 ctgctgctaa agaagaagct tacaaagatg cagttgaagg tgcagcaatt gatacagctg
      901 tagaaaatgc tgaaatcgta gaacttccag aagaaatgat ccacgaagaa gttcaccgtt
      961 cagtaaatga attccttggg aacttgcaac gtcaagggat caaccctgac atgtacttcc
     1021 aaatcactgg aactactcaa gaagaccttc acaaccaata ccaagcagaa gctgagtcac
     1081 gtactaagac taaccttgtt atcgaagcag ttgccaaagc tgaaggattt gatgcttcag
     1141 aagaagaaat ccaaaaagaa gttgagcaat tggcagcaga ctacaacatg gaagttgcac
     1201 aagttcaaaa cttgctttca gctgacatgt tgaaacatga tatcactatc aaaaaagctg
     1261 ttgaattgat cacaagcaca gcaacagtaa aataatctta ataaacagaa aacccacctg
     1321 aattggtggg ttttctgatg cactattttc caaaaatctc tttgaggtct gtgtctgtaa
     1381 tcccaatcat ggctggaatg ctgtcccagt tttcttcggt taggatgtag gattgttcag
     1441 aggcacttga tgtgactgtt tcagagacag cttgttgctt ttcttcaaca ttctccagta
     1501 gatcactgaa gcgttcaatc agataggttt ttcgggcagt tccgatgtgt tgggtagcat
     1561 agtcgaaggc ttgtaattcg cctagtaaga tgagtttgct tttggcacgt gtaatggctg
     1621 tgtagatgag atttcgctcc agcatacgtc ggctagcact agtaatcggt aggatgacaa
     1681 ctgggaactc acttccctga gacttatgaa tactcatggc ataggccaag cgaattttgt
     1741 accattcgtt acgggggtaa gagacttcat taccatcaaa atcaatgaca atctcgtctt
     1801 gtttcgattc ggtgtattta ccaggaatca ggtctgtgat agctcctaaa tccccattaa
     1861 agacattgat ttcagcatcg ttaaccaaat gaatgacctt gtctctctta cgatagtggc
     1921 actgaggagc ttcaaaactg agttgatctt tttgtggggg attgagcagg tcttgcatga
     1981 gctgattgat agcatcaatc cctgccgtcc ctcggtacat aggagccaga acttggatat
     2041 cacgggcagg aataccattt ctgagggcgg cacctaagat tttttcaatg gtggcaggaa
     2101 tatggccact agcaatttca aagtaggaac ggtcagcttt tttttgggtg aaatcagctg
     2161 gcaagatgcc ctgttgaatc tgactagcta gggtgacgat ggttgattct tcgctttgtc
     2221 gataaatttt ttccaagcga gtctgaggaa tcaaaggaat atgaagcaga tccgctagaa
     2281 cctgtccagg actgacagaa ggtagctgat cactgtcacc tacgatgagg atcttactgt
     2341 tagaagagat attggagaag agttgattgg ccagccaagt atccaccata gagaattcat
     2401 ccacgatgat aaagtcagca tctaggtaat cttccagatg actggtatca tcgtcacctg
     2461 tcattcccaa gtggcggtgt atggtcgcgc taggcaaacc tgtcaattca ttcatgcgac
     2521 gagcagctcg accagttgga gcagcaagaa gaatgggcag gttgcttttc ttcctgaggt
     2581 caagtccctc taacagggcg taaacagcga tgatcccatt gataacagtt gtcttaccag
     2641 taccaggtcc acctgtcagg ataaagacct tattctggat agcatcacag atagcctgtt
     2701 tttgaatgtt atcatactca attcccagct cttcctcgat agtagtgata tgtttttgaa
     2761 tggtttctaa atcatgactc ttctgttttc ctttttcaag gatacgaatc aagtgactgc
     2821 ggatgccttc ctcagcgaaa aagaggctgt tgtcaaagat cttggtatca atctgctgaa
     2881 ccttgtcttc ttcgatcagg taggagagct cttgggcaac ttggctgggg tctagttcca
     2941 cgggacggga agactcaagg agagtaaggg tttgttccag caaatcccgt gcttcaacat
     3001 aggtgtcccc tgtttccata caggcctgaa aaagactgtg aactagaccg gcgcggaagc
     3061 gttcaggagc ctgactttcg atgcctagtt cctcagctag ttggtcagca atggtaaagc
     3121 ccaaaccctt gatatcctca accagctggt agggataatt ttcaaccaca tcaagggttt
     3181 cttccttgta aaagtcttga atctgaaagg ctagtttgtt gggaatgccg tagttggcta
     3241 gtttggacaa aaccatctcc gttccgtagt tgagacggag agtggagacg aaagcctcgc
     3301 gatttttggc agagagtcct gtgatgcctt ctaacttttc tgggtgttgc aaaatttcgt
     3361 caatggtatt ttcgccatag gtatccacga ttttctgagc tgtcttgaga ccaatcccct
     3421 tgaaatggct acttgaaaag tacttgacca agcccttact agttggtttt gcgcgatcat
     3481 aacgactgat ttgcagttgt tctccatact tggagtgctg gacaatttgc ccccaaaaag
     3541 tatagtcttc gccctcaatt acatcagcca tggttcctgt gacaatgatt tcaaaatcat
     3601 caaaatcctc tgcgtccgta tcgtcgattt ctaggaggag gatgcgataa aaattgctgg
     3661 gattttcaaa aataatccgt tcaatggttc ctgaaaaata aacttccata aaattccttt
     3721 gcatgaatag gtgagagttg aggtgtttct gttctggtaa gtttagatag ttaccaatca
     3781 ttttctcacg atagaagaag aggctgagat tggtgattct cggcctctta ggtttcttaa
     3841 aatgttccga tacgggtgat tggccagaag cggaatttag cttcccctgt gatatctttt
     3901 gctttgaagg tacctacgtg gcggctgtcg ctcgaaacca agcggtcatc tccgaggaga
     3961 aggtattctc cttctggaac agtaaagcta aagttggtgt tgtagttgac atcaactgtg
     4021 aaggcttggg ctttttgagc gatacttcta aagaaagttc ctttatttcc ttcaaagccc
     4081 ttgcctgagt aagtgctttg gagtttgtca tccttgaagc gtttgatata gtctgctaga
     4141 taaggctcgt ccgtttcttt gtcattgatg tagagtttat cattttcgta acgaatggtg
     4201 tcgccaggca ttccaatcac gcgcttgacg atgtccttat tgccatcttc ctcatgggcc
     4261 accacgatat caaaacggtc aataggaagg tgttttacaa cgaagagaat ttcgccatcc
     4321 gctagggtcg gatccatgga atgtccttct acgcgaacat tgctccaaaa aaagatacga
     4381 cttaaagcta gtaatgacag aattaggagg aacaatcccc actcttttaa gaaattttta
     4441 aatgaattca taacttacct ttctaagcgt tttttcgctt tttcagtgtt tttaaagtgc
     4501 aatttggcgc agaagctgag tccctgcata ccataggctt gcaaaatctg gctagccacc
     4561 ttgtcagaag ccgttccagc tccacttggg agctgataac ccagttctcg tcccaaattt
     4621 tcaagatttt ccagaaagag atcacgcgca atgacagaag aaactgcgac agacaagtat
     4681 ttgccctcag ccttttcttc taagctgata ggattgctga aacgattggc ctcttgtgcc
     4741 aagtacttgt cataattttt agcactggta aaggcatcaa tcacaatttt ctcaggctga
     4801 acaccttttt gaaggaggag atagatagcc tgattatgga gggcaacctt aaccgaaaca
     4861 gcattgtagc ggtctccgat gacctcgttg tacttgctgg gtgagagaag gagtgcctgg
     4921 tgctggattt tttccttgag aataggagca atctgacgga tcttttggtc ggtcagagtc
     4981 ttagaatccc ccacaccgag ttttcgtaaa aagtcgtgct ggtcagatgt gacaaaggca
     5041 gccacaactg caagcccacc aaagtaggaa ccatttccca cctcatctgt cccaattaaa
     5101 ggaagatttt gccccctgtt ttcctctaca agttgatagc caaagaaact agcgtatttc
     5161 tcagcccctt caccctgaag caagattttt ccagaagtat agatagaaac cgttgcttga
     5221 ggtagtttca aaaagtagcg gatataggga ttcttactgg gagccagact ggtttgatag
     5281 tgttcaagaa aagcctgaat atccttttcg cttggtgtga gtgtgatact tgccatagtt
     5341 tctattgtac cacaaaagca gtaaaatttg taaaaactga caaaattagc gaattttggt
     5401 ataatatcgt gaggtgaatt ttatggcaaa tctaaatcga ttcaaattta cattcgggaa
     5461 aaaatcgtta accttgacaa gcgaacatga taaccttttt atggaggaaa tcgctaaggt
     5521 tgcgacagaa aaataccaag caattaaaga acaaatgcct agcgcagatg atgaaacaat
     5581 cgctcttttg ttggcagtca actgtttatc aactcagctc agccgtgaga ttgaatttga
     5641 cgataaggag caagagctag aagaactccg tcacaagctt gtgacttgta agcaagaaca
     5701 gagcaagatt gaggattcct tatgatttca ttccttcttc tattggtctt ggtttgggga
     5761 ttttatatcg gctatcggag aggcctgctc ttacaggttt attacctgat ttcagccatg
     5821 gcatcggctt ttatggctgg ccagttttat aaggggcttg gagagcaatt ccatttattg
     5881 ctcccttatg caaattcgca ggaaggtcag gggactttct ttttcccatc ggatcaactc
     5941 tttcagctgg ataaggtctt ttatgcaggt atcggctact tgcttgtatt tgggattgtc
     6001 tatagcattg gtcgtttgct tggtcttctc ttacacttga ttcctagcaa aaaactgggt
     6061 ggtaagttgt tccaagtttc agcaggtatc ttgtccatgt tggtgacctt atttgtcttg
     6121 caaatggcct tgacaatctt ggcgaccatc cccatggcag ttatacaaaa tcctcttgaa
     6181 aagagtatcg tcgcaaaaca catcatccag agcataccga taacaaccag ttggctcaaa
     6241 caaatctggg tgacaaattt aatcggataa aaagggcagg agttttccta gccctttgtt
     6301 tacagatttg actcgaatct atcagaatgt aaaaagctac cacacctaga cattcaaaga
     6361 caaggaaata aagatgaata agaaaatatt agaaacatta gagttcgata aggtcaaggc
     6421 cttgtttgag cctaatttgt tgactgagca gggcttggag caattgagac agctggctcc
     6481 gactgccaaa gcagataaaa tcaaacaggc ttttgctgag atgaaagaaa tgcaggctct
     6541 tttcgtcgag caaccgcatt ttactattct ctcaactaag gaaattgcag gagtctgcaa
     6601 gaggttggag atgggagcgg atctcaatat cgaggagttc ctactcttga aacgcgtgct
     6661 tcttgccagc cgagaacttc aaagttttta tgccaatctg gaaaatgtca gcttggaaga
     6721 attagccctt tggtttgaga aattacatga ttttccgcaa ttacaaggaa atcttcaggc
     6781 ctttaatgat gcgggtttca ttgaaaattt tgccagtgaa gaattggcgc gaatccgtcg
     6841 aaaaatacat gatagcgaga gtcaggtacg cgatgtttta caagacttgc tcaagcaaaa
     6901 agcgcagatg ttgacggaag gaattgttgc tagcagaaat ggccgtcagg ttttaccagt
     6961 caaaaacacc taccgcaata agattgcagg tgtcgttcat gatatttctg ctagtggaaa
     7021 caccgtctat atcgaaccac gtgaggtagt caaactgagc gaagaaattg ctagtctgcg
     7081 agcagatgag cgctatgaaa tgcttcgcat tctccaagaa atttctgagc gtgtccgccc
     7141 tcatgcggct gagattgcta atgacgcttg gattatcggt catctggact tgattcgtgc
     7201 caaggttcga tttatccaag aaagacaagc agtcgtgcct cagctgtcag aaaatcaaga
     7261 gattcaactg ctccatgtct gccatccttt ggtcaaaaat gccgtcgcaa atgatgtcca
     7321 ttttggtcaa gatttaacag ctattgtcat tacaggtccc aatacaggtg ggaagaccat
     7381 catgctcaaa actctgggct tgacacaggt catggcccag tcaggattgc cgattttagc
     7441 agacaaggga agtcgtgttg gtatttttga agaaatcttt gctgatattg gagatgagca
     7501 gtctattgag cagagcttgt ctaccttctc tagccatatg accaatatcg tggatattct
     7561 tggcaaggtc aaccaacatt cactcttact tttggatgag ttgggggctg gtactgatcc
     7621 ccaagaggga gcagcccttg ccatggctat tctggaggac cttcgcctgc gtcaaatcaa
     7681 gaccatggcg acgacccact atccagaact caaggcctac ggtattgaga cagcctttgt
     7741 gcaaaatgcc agtatggagt ttgatactgc aactcttcgc ccgacctatc gctttatgca
     7801 gggtgttcct ggccgaagta atgcctttga aattgccaaa cgtctaggcc tatctgaagt
     7861 tatcgtagga gatgccagtc agcagatcga tcaggacaat gacgtcaatc gtatcattga
     7921 gcaattagaa gagcagacgc tggaaagccg caaacgtttg gacaatatcc gtgaggtgga
     7981 gcaagaaaat ctcaagatga accgtgcgct aaaaaaactc tacaacgagc ttaatcgtga
     8041 aaaggaaacc gagcttaaca aggcgcgtga acaggctgct gagattgtgg atatggccct
     8101 aagtgaaagt gaccagattc tcaaaaatct ccacagtaaa tcccaactca agccccacga
     8161 aatcattgaa gccaaggcca agttgaaaaa attggctcct gaaaaagtgg acttgtctaa
     8221 aaataaggtc cttcaaaagg ccaagaaaaa acgagctcca aaggtgggag atgatatcgt
     8281 ggttctcagt tatggtcagc gtggtacctt gaccagtcaa ctcaaggacg gtcgctggga
     8341 agcccaagtt ggcttgatta agatgacctt ggaagagaaa gagtttgatc ttgttcaagc
     8401 ccagcaagaa aaaccagtca agaagaaaca ggtcaatgtt gtgaaacgaa cttctgggcg
     8461 aggacctcaa gctagactgg atcttcgagg caagcgctat gaagaagcca tgaatgagct
     8521 agataccttc atcgaccaag ccttgcttaa caatatggct caagttgata tcatccatgg
     8581 tatcggaaca ggagtcatcc gtgaaggagt taccaaatac ttgcaaagaa acaaacatgt
     8641 caagagtttc ggctatgccc cacaaaatgc tggaggcagt ggtgcgacta ttgtcacttt
     8701 taaaggatag cagtattctg gactttataa agtaaaaact gttgaactaa tttttactaa
     8761 taaacacatt gacaaaagcc aacatttttt gtaaaattag aatcaattaa ataccaacac
     8821 cgaatgaagt ttaatagaag tggggaatcg tttgattttc catgactgta aatggacgga
     8881 actctggaga gaccgtaaag gcaccgaagg ggcaaggcag gcaactgctc aaactctcag
     8941 gtaaaaggac agagctagga tagaccgctt tttagcattt atctaagcat tccagagtac
     9001 atgtatcttg catgtgctct ttcttttggg gttgaaacga taggagaagg aaatgttaga
     9061 attgcttaaa tcaatcgatg cttttgcttg gggaccgccc ctcttgattt tattggtcgg
     9121 aacagggatt tacctaacca tgcggctagg actcttgcag gttttgcgtc tgcccaaggc
     9181 ctttcagctt atttttatcc aggataaggg acatggtgat gtatccagtt ttgcagctct
     9241 gtgtacagcc ttggcatcaa ctgttggaac aggaaatatc ataggagttg cgacggctat
     9301 caaggttggt ggaccaggag ctctattttg gatgtggatg gcggctttct ttggaatggc
     9361 taccaagtat gcggaaggac tcttggccat caaataccgc accaaggacg accatggtgc
     9421 agtagcggga ggtcccatgc actatatcct tctagggatg ggagaaaagt ggcgaccact
     9481 tgctgttttg tttgcagtag caggagtatt ggttgctctc ttgggaatcg gaaccttcac
     9541 ccaagtcaac tcgattacag aatctatcca aaatacaacg acgatttcgc cagccatcac
     9601 agctctcgtc ttgtctgtct ttgtagcgat tgcagtcttt ggtggactca agtctatttc
     9661 taaggtttca actactgttg ttccttttat ggccctcatt tatatcttag gaactcttac
     9721 agttattttc tttaatatcg gaaaaatccc tggcacaatc gctttagtct ttacctcagc
     9781 ttttagtccc cttgctgcgg taggtggatt tgctggtgct agcgttcgga tggctattca
     9841 aaatggtgtg gcgcgtggtg tgttctcaaa cgaatctggt ctgggttctg ctcctattgc
     9901 agctgcagct gccaagacaa atgaaccagt agagcaaggt ttgatttcca tgacaggaac
     9961 ctttattgat accctcatca tttgtactct aactggtttg accatcttgg taactggagt
    10021 ttggagtggt gacttgaatg gggttgcctt gactcagtca gctttctcaa cagtcttttc
    10081 acactttggg cctgccctct tgaccatctt ccttgtactt tttgccttta caacgattct
    10141 aggttggaac tattacggag aacgctgttt tgagttcctc tttggggctc gctttatctg
    10201 gctctaccgt gtggtttttg tgctcatggt cttgttagga ggatttatcg agttggatat
    10261 ggtctggatt atcgcagata tcgttaacgc cttgatggct ctgccaaact tgattgccct
    10321 cttggtcttg tcgccagtcg ttattgctga aactaaaaag tattttgaca aataatggaa
    10381 tcacacagct cgtgtgattt ttttgcctat aaataatttt tatcagcctg atatataata
    10441 tatattatgc aagtgattgg aagtgtgata aactaatata acaacgaaaa tcttatgaga
    10501 gaataaagct ttatcttaga taaacttagg aatcaggatt ttataagatt ttccaataat
    10561 attagtgtca ataataagaa aagaggtatc ttatgacaac atttactatc catacagtag
    10621 agtcagcacc agcagaagtg aaagaaattc ttgaaacagt agaaaaagac aacaatggct
    10681 atattcccaa cctaatcggt ctcttggcca atgccccgac tgttttagaa gcctaccaaa
    10741 ttgtctcatc tatccaccgt cgcaacagcc tgacacccgt tgagcgtgaa gtggtgcaaa
    10801 tcacggcagc cgtgaccaat ggttgtgcct tctgtgtcgc aggtcacaca gccttttcca
    10861 taaaacaaat ccagatgaat gatgacttga ttcaagctct tcgcaatcgt actccaattg
    10921 aaacagatcc taaattggat accctagcta agtttacctt ggcagttatc aataccaagg
    10981 gtcgtgtagg agatgaagcc ttgtctgagt ttttagaagc tggctacact caacaaaatg
    11041 ccttggatgt ggtttttggt gtcagcctag caatcctctg taactatgcc aacaacttag
    11101 ctaatacacc aattaatcca gaattgcaac cttatgccta attcatatct gagtaaaatg
    11161 aagtctgaaa taagcggact tcgttttttt aaatcctcat ttaagcgctt ttatgttata
    11221 ctgaaactaa catgattatt ggaggttagg atgaaaaaac tccccttagt attttcaggt
    11281 tgtttgctag gtttggcagg agctggaaat cttattttag atacgttgcc ggttctatcc
    11341 catcttttta gtctgactgg tttggtttta tggatttact ttctaattct gcatctcttt
    11401 aattggaaag aaaccaagca agaattgacc aagccccctc ttttgtcagg aatggcaacc
    11461 tttcctatgg ctgggatgat tttatcgacc tatgtctttc gcgtattctc ttatcttcct
    11521 ttggtagcac aagggatttg gtggttttca tttctcttgg atttgacctt gattgctggt
    11581 tttaccatca agtttgcttg tccagggcgg agggttcatg ccactccaag ttggacggtt
    11641 ctctatgtgg ggatagcagt ggctgccttg acctatcctc tggtaggtat tatcgaaatt
    11701 gcctatgcga ccttgagttt tggttttctc ttgaccttct atctctatcc ccttatttat
    11761 agcgatttaa agaaacatcc actcccacta gccttgcttg gacaagaagg aatctactgt
    11821 gctcctttct ctctactctt ggcttctcta gttcgagtag gaggaaccag cctgccgact
    11881 tgggtcttga ttgtcatgat tttggcttct caatccttct ttttctttgt tttaactcgt
    11941 ctgcccaaca ttttaaaaca aggttttcaa ccagccttct cagccctcac cttcccaacc
    12001 attatcacag cgacctcgct caagatggct cagggaattt tgaaacttcc atttctggat
    12061 tacctggtat tggctgaaac cattatatgc ctaactattt tattctttgt actaggtgct
    12121 tatctgattt ggttacgaaa aaggtctagc tagaaatagc taaaccttat tttttatggt
    12181 ttgatgactt cagctccacc catgtatgga cgaagtgctt ctgggatggt cacagaacca
    12241 tcttcatttt ggtaattttc aagaattgca gccactgtac gtccaactgc aagtccagaa
    12301 ccgttcaagg tatgaaggag tttcaccttg ccatctgctt catcacggta acggatttgg
    12361 gcacgacggg cttggaaatc ttctgtgttt gaacagcttg agatttcacg gtaattgttt
    12421 tgtgctggaa tccacacttc caagtcgtaa gtcttcgtag ctgagaagcc catatctcca
    12481 gtagagagag caacgacacg gtatggaagg ttgagttttt gaagaatgtt ttcagcgttg
    12541 gctgtcattt tttccaattc ttcgtaagat tcttctggtt tggcaaattt gaccatttca
    12601 accttgtgga attggtgcaa acggatcaag ccacgcgtat cacgaccggc agaaccagcc
    12661 tcagaacgga atgacggact catagcagtg aagtagattg gaagatcttt gccgtctaag
    12721 atttcatcac ggtagtagtt tgtcagagga acttcagcag ttggaatcaa gacaaagttg
    12781 gtatcgctga gttcaaaagt atcttcctta aattttggat actgaccagt accaaacata
    12841 gaatcatggt tgactatgta aggtgtgatg acttcagtat agccttcttt tccatgttca
    12901 tccaacataa agttgtagat agcacgttcc aaacgagcac cgaggccttt atagaagagg
    12961 aagcgagcgc ctgttacctt accaccgcgt tcccagtcaa ggataccaag gtcttcaccg
    13021 agatcccagt gagctttagg ttcgaagtca aactcgcgtg gagtacccca acggcgaact
    13081 tccacattgt cgtcttcgtc agtcccaaca ggaacgctgt cagctgggat atttggaaga
    13141 gtcgttgtaa attctgtcaa tttagcatcg atttctgcca attcagcatc caaggcttta
    13201 acctcagcag atagattttg catggcagca atcttgtcat ctgtattttc cttgttgcgc
    13261 ttagcttggg caatctcagc agaaactgtg ttacgttctg ctttgagagt ttcaaccttg
    13321 accaagatgt tacgacgttt agcatcgatt tctttcattt cattcaagac agcagcatct
    13381 acaccacgtg tagctaattt ttctgcgaca gcttcaaaat ctgtacgaat acgtttgata
    13441 tctaacataa gaactccttt atgaaaaaag cacgcctgac aaaacgttgg agtggcaggg
    13501 ccacggttcc atccaacttc acaggtgtgc acttgattgt gtatgtaatt gttactaacg
    13561 gtagaatttc acctatcccc cctatctgct cgcagtaccc gcagactttc tgaaagaaga
    13621 agataaccta cttatccgtt gctatgatta tactaaagtt tctacttttt tgcaaataga
    13681 tttttaaatt tttggctaat tgtctgaatc agggtcggaa gtttgacgac cttgtcattg
    13741 cctagttttt cgcgtgcaat tttgagaatg gcacctgagt cttttgaagc aaagaggaat
    13801 tttcctttgt ctgtaaagac ttcgaagtgg cggctgactt tgcgtccagt gacattggct
    13861 ccaatctgat tgatatggct ccaaggaatc tggataaatt gttcgacatt gacatctggg
    13921 taaaattcca aagcctgatc tccgacaagg aatttcccaa ctttcccagc gatagagagg
    13981 taggaagtgc ctgtcgtact gaggagtact gttttgttaa gtgattgggc catgcttagt
    14041 cttccttact ttctccaaaa aaggcattgt agagggcttt aattgctgct ttctcttggt
    14101 ctttattgac aacaaacata atagaaactt cactagaacc ttgagacatc atctggatgt
    14161 tgattttgtt ttcagataga gcgcgtgtcg cagtagcagt cactccgata tggctcttca
    14221 ttttttcacc aacaatcata atgatagaaa ggtcgtgttc gatttctgca tgatctactt
    14281 tagccttttg aaccaactga cgcaggattt cttcttcctt gatgggagtt agttggcgag
    14341 aacggagaat gatagaaaga tcgtcgatac ctgttggcat atgttcccaa ccgatgttaa
    14401 gttcttccag gatttgcaga accttgcggc caaatccaac ctcacggttc atgaggtatt
    14461 tcgacatgtt aatgctgaca aagcctgagt caccagcaat tcccacaact ggaaattcat
    14521 cattactgtg ttttagaacg atacgagtac ctggatggtc agggttgttg gtattcttga
    14581 taaccagagg aatttttcca cggtaggcag gaagaagagc ctcgtcatga aggactgaga
    14641 agcctgcata ggccaactcg cgcatttcac ggtaggtcaa ctcaggaatc gagtgtggtt
    14701 ggtggataat accagggtgg gctgcaaaga taccatcaac gtccgtaaag ttttcataga
    14761 ggtcagcttt gacaccagca gcaatgatag aacctgtaat atcagaacct ccacgtgaga
    14821 aagtacagat ttgattttcc ttagtgacac caaagaaacc aggaatgaca aggacttcat
    14881 tggtgtttgt caattcttca atcttgtcat aacttgatgg aatgatgcga gcgtgaccag
    14941 gttcacttgt gaccacaatc ccagcttctc tagggtgcat atagcgtgca tcgataccat
    15001 tttggttaaa gtaggcagca atcaatttgg cattgttatt ttcaccggct gctaggaaag
    15061 tatcgtagag aaattcattt tcttcaatag gaagagtggc caaggcgtga atgcttttag
    15121 aaattttttc tagcacagct ggttttagtc ccaattcact aaccatagca gcatagcggt
    15181 cgataatcca gctttggctc ttgctaatat cgttacccgc aacatagtcg cggtagtatt
    15241 taatcagggc atccgtaacc ttagtatctt cagcattacg tttaccaggc gcagaaacga
    15301 ctacaaaacg acgctctgaa tcgcttttga cgatgtttaa aactttttct aattgactag
    15361 cagaggcaag agaactacct ccaaatttaa caactttcat aagaactcct aaagtaagta
    15421 ttttatacga ttatagcaga aagaaaggct tttttcaatg aagaaaataa gatgctttct
    15481 agcctaaagc gagcctttct tatcggctga gacactttca gtcgattttt tcttgctttc
    15541 tgctcctaaa aaagatatac tttgacagtg aaataattta acactcaatg aaaatcaaag
    15601 agcaaactaa gaagctagac gaagtcagct caaaatactg ttttgaggtt gcagatggaa
    15661 gctgacgcgg tttaaagaga ttttcgaaga gtataaactg cttataaaat aaaaaggagc
    15721 cctgatggaa cacattattt atcagcttga agaggacttg gcaatcctta ccttgaaccg
    15781 tcctgaggtc gcaaatggtt ttcatattcc catgtgtgag gagattttag aagctctgac
    15841 tttggcagaa gaaaatccag ctgtgcattt tatcttaatc aatgccaatg gaaaggtctt
    15901 ctcagttggg ggagatttgg tagagatgaa gcgggcagtg gatgaggatg atattccatc
    15961 attgacaaaa atcgcagagt tggtcaatac gatttcttat aaaatcaagc aaatagccaa
    16021 acctgtttta atggaagttg acggtgctgt tgcaggtgcc gcagcgaata tggccgttgc
    16081 ggcagatttc tgtttggcga cggataaggc taagtttatc caagcctttg ttggtgttgg
    16141 tttggctcca gatgcagggg gaattcatct cttgagccgc agtatcggtg tgacgcgtgc
    16201 tgctcaatta gctatgacag gagaggcttt aacggcagaa aaagccctag aatgggggtt
    16261 ggtttaccgc gtttctgaag ctgaaaaact tgaaaagacg agagaacagc ttcttaaaaa
    16321 attaagacgt gcttcaagta attcctatgc tgccattaag aagttggttt gggagagcca
    16381 atttaaagac tggcagggtt acgctacttt agaactgaac ctacagaaat ccttagctca
    16441 aacagaggat ttcaaagaag gagtgcgagc tcattcggag agaagaagac ctaaatttac
    16501 aggaaaataa aaaatacttg catcattctt tgaattttga tataattctt tctgtcaaat
    16561 gttttgattg taaaagtttt ttgaagaagg agggatatga aaaatggact accaacgaat
    16621 caatgaatat ttaacatcta tatttaacaa tgtccttgta attgaggaag tgaacttgag
    16681 aggtagtcgt tttaaggata tctccatcaa agaaatgcat acgattgatg tcatcggtaa
    16741 ggctccagat gtgactccaa gtcaagtgtc aaaagagttg atggtaactc ttggaactgt
    16801 tacgacaagt ttgaacaatt tagagcgtaa gggctacatt gagcgagttc ggtcagaaca
    16861 ggatcgtcgt gtggtgcatc tgcatttgac aaagaagggt cgcttgattc atagactgca
    16921 taaacgcttc cacaaggcca tggtagaaaa aattattgat ggcatgagcg aggaagaaat
    16981 tgctgtcatg ggtaaaggtt tgactaatct ttaccaattt ttggaggatt tgaaataatg
    17041 gcttttgcaa aaataagtca ggttgctcat tatgtgccag agcaagtggt tacaaatcac
    17101 gacttggctc agattatgga taccaatgat gagtggattt caagtcgaac gggaatacga
    17161 caaaggcata tttcaagaac agaatctacc agtgatttgg ctacagaggt tgctaagaaa
    17221 ctgatggcaa aagctggaat aacaggagaa gaactggatt ttatcatcct agctaccatt
    17281 actccagatt cgatgatgcc ctctacagct gctcgtgttc aagctaatat tggtgctaat
    17341 aaagcctttg cttttgactt aaccgcggct tgcagtggat ttgtatttgc tctttcaact
    17401 gctgaaaagt ttatcgcttc tggtcgcttt caaaaaggct tggtgattgg tagtgaaacc
    17461 ctctctaagg cagtcgattg gtcggatcga tcaacagctg tgttgtttgg agatggtgct
    17521 ggtggtgtct tgttagaagc tagcgagcaa gagcatttct tagctgagag tctcaatagc
    17581 gatggaagtc gcagcgagtg tttaacttat gggcattcag gtttgcattc tccattttca
    17641 gatcaagaaa gtgcagattc atttttgaag atggatggac gcacagtctt tgattttgcc
    17701 attcgagatg tagccaagtc tatcaagcag actattgatg aatctcctat agaggtgaca
    17761 gacttggatt atctgctact tcatcaagcc aatgaccgta ttttggataa gatggctaga
    17821 aaaattggtg ttgaccgagc caaacttcca gccaatatga tggaatatgg caataccagt
    17881 gcagccagta tcccgatttt actttcagag tgtgtagaac aaggtctcat ccatttagat
    17941 ggtagccaga ctgttcttct atcaggcttc ggtggaggtt tgacctgggg cacgctcatt
    18001 cttacaattt aggtaatcat gtggtgaaca cattgttata aaaaaaaact atttatttta
    18061 aaggagtcct atcatggcag tatttgaaaa agtacaagaa attatcgttg aagaacttgg
    18121 aaaagacgca tcagaagtaa cacttgaatc aacttttgat gatttggacg cagattcatt
    18181 ggacttgttc caagtaatct cagaaatcga agatgctttt gatatccaaa tcgaagcaga
    18241 aaatgacttg aaaacagttg gtgacttggt tgcttacgtt gaagagcaag caaaataagc
    18301 agaatattag tagaaggagt agggagaaac ccactccctc ttgtttaggt aatagtttga
    18361 ctgtcaaatt atggtgaaat cgaactatta cgtaagcaag gaaggttgga ggcactatga
    18421 aaacgcgtat tacagaatta ttgaagattg actatcctat tttccaagga gggatggcct
    18481 gggttgctga tggtgatttg gcaggggctg tttccaaggc tggaggatta ggaattatcg
    18541 gtgggggaaa tgccccgaaa gaagttgtca aggccaatat tgataaaatc aaatcattga
    18601 ctgataaacc ctttggggtc aacatcatgc tcttatctcc ctttgtggaa gatatcgtgg
    18661 atctcgttat tgaagaaggt gttaaagttg tcacaacagg agcaggaaat ccaagcaagt
    18721 atatggaacg tttccatgaa gctgggataa tcgttattcc tgttgttcct agtgtcgctt
    18781 tagctaaacg catggaaaaa atcggtgcag acgctgttat tgcagaagga atggaagctg
    18841 gggggcatat cggtaaatta acaaccatga ccttggtgcg acaggtagcc acagctatat
    18901 ctattcctgt tattgctgca ggaggaattg cggatggtga aggtgctgcg gctggcttta
    18961 tgctaggtgc agaggctgta caggtgggga cacggtttgt agttgcaaaa gagtcgaatg
    19021 cccatccaaa ctacaaggag aaaattttaa aagcaaggga tattgatact acgatttcag
    19081 ctcagcactt tggtcatgct gttcgtgcta ttaaaaatca gttgactaga gattttgaac
    19141 tggctgaaaa agatgccttt aagcaggaag atcctgattt agaaatcttt gaacaaatgg
    19201 gagcaggtgc cctagccaaa gcagttgttc acggtgatgt ggatggtggc tctgtcatgg
    19261 caggtcaaat cgcagggctt gtttctaaag aagaaacagc tgaagaaatc ctaaaagatt
    19321 tgtattacgg agccgctaag aaaattcaag aagaagcctc tcgctgggca ggagttgtaa
    19381 gaaatgacta aaacagcctt tttatttgct ggtcaaggtg cccagtatct agggatggga
    19441 cgggatttct atgatcagta tccgattgtc aaagaaacga ttgatcgagc gagtcaggtg
    19501 ctcggttatg atttacgtta tctcatcgat acggaagaag acaaactcaa tcagacccgc
    19561 tatacgcaac cagccattct agcgacttcg gttgctatct accgtttatt gcaagaaaag
    19621 ggctatcagc ctgatatggt tgctggtttg tctcttggag aatactctgc cttggtggca
    19681 agcggcgcct tggattttga agatgcggtt gccttggtag ctaagcgtgg agcctatatg
    19741 gaagaagcgg ctcctgctga ctctggcaag atggtagcag ttctcaatac gccagtagag
    19801 gtcattgaag aagcctgtca aaaagcttct gaacttggag tggttactcc agccaactat
    19861 aacacacctg cacaaatcgt cattgctgga gaagtggttg cagttgatcg agcggttgaa
    19921 cttttgcaag aagcaggtgc caaacgcttg attcctctta aggtgtcagg tccctttcac
    19981 accgctctcc ttgagcctgc tagccagaaa ctagctgaaa ctctagctca ggtaagtttt
    20041 tcagatttta cttgtcccct agtcggcaat acagaagctg ctgtgatgca aaaagaggac
    20101 attgctcagc tcttgacgcg tcaggtcaag gaacccgttc gtttctatga aagtattggg
    20161 gtcatgcaag aagcaggcat aagcaacttt atcgagattg gaccggggaa agtcttgtca
    20221 ggttttgtta aaaaaattga tcaaactgct cacttagctc atgtggaaga tcaagcgagt
    20281 ttagtagcac ttttagaaaa atagactaaa ataagtagaa gttttgaaag gaaaaaaatg
    20341 aaactagaac ataaaaatat ctttattaca ggttcgagtc gtggaattgg tcttgccatc
    20401 gcccacaagt ttgctcaagc aggagccaac attgtcttaa acagtcgtgg ggcaatctca
    20461 gaagaattgc tcgctgagtt ttcaaactat ggtatcaagg tggttcccat ttcaggagat
    20521 gtatcagatt ttgcagacgc taagcgtatg attgatcaag ctattgcaga actgggttca
    20581 gtagatgttt tggtcaacaa tgcagggatt acccaagata ctcttatgct caagatgaca
    20641 gaagcagatt ttgaaaaagt gctcaaggtc aatctgactg gtgcctttaa tatgacacaa
    20701 tcagtcttga aaccgatgat gaaagccaga gaaggtgcta tcattaatat gtctagtgtt
    20761 gttggtttga tggggaatat tggtcaagct aactatgctg cttctaaggc tggcttgatt
    20821 ggctttacca agtctgtggc acgcgaggtc gctagtcgga atatacgagt caatgtgatt
    20881 gctccaggaa tgattgagtc tgatatgaca gctatcttat cagataagat taaggaagct
    20941 acactagctc agattccgat gaaagaattt gggcaggcag agcaggttgc agatttgaca
    21001 gtatttttag caggccaaga ttatctaact ggtcaagtgg ttgccattga tggtggctta
    21061 agtatgtagc gaaagctaga ggtgaaaaga atgaaactaa atcgagtagt ggtaacaggt
    21121 tatggagtaa catctccaat cggaaataca ccagaagaat tttggaatag tttagcaact
    21181 ggggaaatcg gcattggtgg cattacaaaa tttgatcata gtgactttga tgtgcataat
    21241 gcggcagaaa tccaagattt tccgttcgat aaatactttg taaaaaaaga taccaaccgt
    21301 tttgataact attctttata tgccttgtat gcagcccaag aggctgtaaa tcatgccaat
    21361 cttgatgtag aggctcttaa tagggatcgt tttggtgtta tcgttgcatc tggtattggt
    21421 ggaatcaagg aaattgaaga tcaggtactt cgccttcatg aaaaaggacc caaacgtgtc
    21481 aaaccaatga ctcttccaaa agctttacca aatatggctt ctgggaatgt agccatgcgt
    21541 tttggtgcaa acggtgtttg taaatctatc aatactgcct gctcttcatc aaatgatgcg
    21601 attggggatg ccttccgctc cattaagttt ggtttccaag atgtgatgtt ggtgggagga
    21661 acagaagctt ctatcacacc ttttgccatc gctggtttcc aagccttaac agctctctct
    21721 actacagagg atccaactcg tgcttcgatc ccatttgata aggatcgcaa tggctttgtt
    21781 atgggtgaag gttcagggat gttggttcta gaaagtcttg aacacgctga aaaacgtgga
    21841 gctactatcc tggctgaagt ggttggttac ggaaatactt gtgatgccta ccacatgact
    21901 tctccacatc cagaaggtca gggagctatc aaggccatca aactagcctt ggaagaagct
    21961 gagatttctc cagagcaagt agcctatgtc aatgctcacg gaacgtcaac tcctgccaat
    22021 gaaaaaggag aaagtggtgc tatcgtagct gttcttggta aggaagtacc tgtatcatca
    22081 accaagtctt ttacaggaca tttgctgggg gctgcgggtg cagtagaagc tatcgtcacc
    22141 atcgaagcta tgcgtcataa ctttgtacca atgacagctg ggacaagtga agtatcagat
    22201 tatatcgaag ctaatgtcgt ttatggacaa ggcttggaga aagaaattcc atacgctatt
    22261 tcaaatactt ttggttttgg aggccacaat gcagttcttg ctttcaaacg ttgggagaat
    22321 agataagtat gaatttaaac gatattaaag acttgatgac tcaatttgac cagtcaagtt
    22381 tgagagaatt ttcttataaa aatgggacgg atgagttgca gtttagcaag aatgaagcga
    22441 gacctgtgcc tgaagttgca actcaagtcg ctccagcacc cgttctagca acaccgagtc
    22501 cagtagctcc tacatctgct ccagcagaga ctgtagcaga agaagttcca gctccagctg
    22561 aagcaagtgt ggctactgag ggaaatcttg tagagagtcc acttgttgga gtggtttact
    22621 tggctgctgg accagataaa cctgccttcg ttacagttgg tgatagtgtc aaaaaaggtc
    22681 aaacattggt aattatcgaa gccatgaaag tcatgaatga aatcccagct cctaaggatg
    22741 gtgtggtaac ggaaattctc gtctctaacg aagaaatggt tgagtttggt aaaggattgg
    22801 tacgtatcaa atgatcgata ttcaaggaat caaagaagct cttccccacc gttatcctat
    22861 gcttctagtg gaccgtgtct tggaagtgag cgaggatacc attgttgcta tcaaaaatgt
    22921 gaccattaac gaacctttct ttaacggcca ctttcctcaa tacccagtta tgccaggtgt
    22981 tctgattatg gaagccttgg cgcaaactgc tggtgtgttg gagttatcaa aacctgaaaa
    23041 taaaggaaaa ctggtctttt acgctggtat ggacaaggtt aagttcaaga agcaagttgt
    23101 accaggcgac caattggtta tgacagcgac ttttgtaaaa cgtcgtggca ccatagctgt
    23161 ggttgaagca aaggctgaag tggatggcaa gcttgcagcc agtggtatcc ttacttttgc
    23221 aattgggaac taaggaggtt ctccatgttt cgaaaaattt taattgccaa tcgtggtgaa
    23281 attgcggttc gtattatccg tgcggcacgt gaattgggga ttgcgacggt agcggtttat
    23341 tcaactgctg ataaggaagc tcttcatacg cttttggcag atgaagcagt ttgtattggt
    23401 cctggcaagg caacagagtc ttatctcaat attaatgcag ttctatcagc tgcagtcttg
    23461 actgaggcag aagctattca ccctggtttt ggatttctca gtgaaaattc caaatttgcg
    23521 accatgtgtg aagaagtagg tatcaagttt atcggtccat ctggtcatgt tatggatatg
    23581 atgggggata agatcaatgc gcgtgctcag atgattaaag caggtgtgcc tgttatacca
    23641 ggttcagatg gagaagtgca taactctgaa gaagctttga ttgttgctga aaaaattggc
    23701 tatcctgtta tgctcaaggc ttcagcaggt ggaggtggta aagggattcg taaggttgaa
    23761 aaaccagatg acctcgtttc tgcctttgaa actgcctcta gtgaggccaa ggccaattat
    23821 ggcaatggtg ccatgtacat agaacgggtt atctatccag ctcggcacat tgaggttcaa
    23881 atcctaggtg atgagcatgg acatgtgatt cacttgggtg aacgggattg ttctcttcaa
    23941 aggaataacc aaaaggtttt ggaagaaagt ccctcgattg caatcggaaa aacgctgcgt
    24001 catgaaatag gtgctgctgc tgttcgagcg gcagagtttg ttggctatga gaatgcagga
    24061 accattgaat ttcttcttga tgaagcaagt agcaatttct atttcatgga gatgaatact
    24121 cgtgttcagg tagaacatcc agtaacagag tttgtttcag gtgttgatat cgttaaggaa
    24181 cagatttgca ttgcggcagg tcagcctttg tctgttaagc aagaagatat tgtcctacgc
    24241 ggtcatgcca tcgagtgtcg tatcaatgca gaaaacccag cctttaactt tgctccaagt
    24301 ccaggtaaga ttactaatct ctatctgcca agtggtggag ttggcttgcg cgtggattca
    24361 gcagtttatc caggttatac cattccgcct tattatgata gtatgattgc caaaatcata
    24421 gtacacggcg aaaatcgttt tgacgccttg atgaaaatgc aacgtgccct ctatgaatta
    24481 gagattgaag gagtgcagac caatgcagat ttccagcttg acctcatttc agatcgcaat
    24541 gtcattgctg gggattatga tacttccttc ttgatggaaa ccttcttacc taaatatcaa
    24601 gaaaaagaat aaaatgactt taagagttta aaccgaaaag gggaatcaat ggctctattt
    24661 agtaaaaaag ataagtatat tcgaatcaat cccaatcgtt cggttaggga aaaacctcaa
    24721 gctaagccag aggttccaga tgaattattt tcccagtgtc caggctgtaa gcataccatc
    24781 tatcagaagg atctgggaag tgaacgtatc tgtccgcact gtagctatac ctttcgtatt
    24841 tctgcccaag aacgcttggc tttgacgatt gatatgggaa ccttcaaaga attgtttaca
    24901 gggattgaaa gcaaggatcc cttgcatttc cctggttacc aaaagaaact ggcatctatg
    24961 cgtgaaaaaa caggtctgca tgaagccgtt gtgacaggaa ctgctcttat taaaggtcag
    25021 actgtagctc ttgggattat ggattctaac tttatcatgg cttctatggg tacggttgta
    25081 ggtgaaaaaa tcactcgttt gtttgagtat gcgactgtcg aaaaattgcc agttgtccta
    25141 ttcacagcct ctggtggagc ccgtatgcag gaaggaatca tgagtctcat gcagatggct
    25201 aagatctctg cggcggttaa acgccattca aatgctggtc tcttttacct gaccattttg
    25261 acagatccaa cgactggtgg tgtgacagct tctttcgcta tggaaggcga tatcattctg
    25321 gctgaaccac agagcttggt tggttttgct gggcgtcgtg tgattgaaaa tacggttcgt
    25381 gaaagcttgc ctgaggattt ccaaaaggca gaattcctat tagaacatgg ctttgtggat
    25441 gctattgtca aaagaagaga cttaccagat acgattgcta gcctagtcag attgcatgga
    25501 gggagtccta gatgaatatt gcaaaaatag tcagagaagc gcgtgagcag agtcgcttga
    25561 caaccttgga ctttgcgaca ggcatttttg atgaatttat ccaattacat ggtgaccgtt
    25621 cttttcgtga tgatggtgca gttgttggtg gtattggttg gcttggagac caagctgtaa
    25681 cagtggttgg tatccaaaaa ggcaagagtt tgcaagacaa cctcaaacgg aattttggcc
    25741 aaccacatcc agaaggctac cgaaaggcac tgcggttgat gaaacaggct gagaaatttg
    25801 gccgtccagt tgtgaccttt attaatacag caggtgctta tcctggtgtc ggagcagaag
    25861 aacgtggtca aggggaagct atcgctcgca atctcatgga aatgagtgac ctgaaagttc
    25921 ctattatcgc cattattatc ggtgaaggtg gttcaggcgg ggctctggct ctagctgtcg
    25981 cggaccgtgt ctggatgctg gaaaattcta tctatgccat tctcagtcca gaaggttttg
    26041 cttccatttt atggaaggac ggtactcgcg ccatggaagc agcagaactg atgaaaatca
    26101 cttcgcatga actgttagaa atggacgtgg tggataaggt gatttctgaa gtaggacttt
    26161 ctagtaaaga actgattaag agtgtcaaaa aagaactcca aacggagctg gctagacttt
    26221 cacaaaaacc gctagaagag ttgctggaag aacgctatca acgatttaga aaatactaag
    26281 aatgactaaa tgagactaga aaggtctcat tttttatgga aaaatgcaat tcaggttgaa
    26341 aaattgatag ttgaggagat gggctataaa atagacaaaa aagatgtata ctgtagatgt
    26401 aagcttacaa aaa
//
LOCUS       DAVMEF010000007        25402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMEF010000007 DAVMEF010000000
VERSION     DAVMEF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.007"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            673..2652
                     /locus_tag="VYQ30_000202"
     CDS             673..2652
                     /locus_tag="VYQ30_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1965376.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2719..4230
                     /locus_tag="VYQ30_000203"
     CDS             2719..4230
                     /locus_tag="VYQ30_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965377.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKEANNVYNR"
     gene            4214..5140
                     /locus_tag="VYQ30_000204"
     CDS             4214..5140
                     /locus_tag="VYQ30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965378.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            5149..6216
                     /locus_tag="VYQ30_000205"
     CDS             5149..6216
                     /locus_tag="VYQ30_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965379.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            6227..7153
                     /locus_tag="VYQ30_000206"
     CDS             6227..7153
                     /locus_tag="VYQ30_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965380.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7772..8581
                     /locus_tag="VYQ30_000207"
                     /pseudo
     CDS             7772..8581
                     /locus_tag="VYQ30_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8593..9486
                     /locus_tag="VYQ30_000208"
     CDS             8593..9486
                     /locus_tag="VYQ30_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965381.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVSNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            9499..10302
                     /locus_tag="VYQ30_000209"
     CDS             9499..10302
                     /locus_tag="VYQ30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1965382.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMNLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(10335..11045)
                     /gene="treR"
                     /locus_tag="VYQ30_000210"
     CDS             complement(10335..11045)
                     /gene="treR"
                     /locus_tag="VYQ30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1965383.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            11230..13197
                     /gene="treP"
                     /locus_tag="VYQ30_000211"
     CDS             11230..13197
                     /gene="treP"
                     /locus_tag="VYQ30_000211"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1965384.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            13364..14989
                     /gene="treC"
                     /locus_tag="VYQ30_000212"
     CDS             13364..14989
                     /gene="treC"
                     /locus_tag="VYQ30_000212"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1965385.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            15245..15493
                     /locus_tag="VYQ30_000213"
     CDS             15245..15493
                     /locus_tag="VYQ30_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1965386.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            15700..16494
                     /gene="racE"
                     /locus_tag="VYQ30_000214"
     CDS             15700..16494
                     /gene="racE"
                     /locus_tag="VYQ30_000214"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1965387.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            16491..17462
                     /locus_tag="VYQ30_000215"
     CDS             16491..17462
                     /locus_tag="VYQ30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1965388.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            17438..17959
                     /locus_tag="VYQ30_000216"
     CDS             17438..17959
                     /locus_tag="VYQ30_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1965389.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17956..18417
                     /locus_tag="VYQ30_000217"
     CDS             17956..18417
                     /locus_tag="VYQ30_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1965390.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            18408..19148
                     /gene="xerD"
                     /locus_tag="VYQ30_000218"
     CDS             18408..19148
                     /gene="xerD"
                     /locus_tag="VYQ30_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1965391.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            19148..19876
                     /locus_tag="VYQ30_000219"
     CDS             19148..19876
                     /locus_tag="VYQ30_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1965392.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19895..20464
                     /locus_tag="VYQ30_000220"
     CDS             19895..20464
                     /locus_tag="VYQ30_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1965393.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            20451..21173
                     /locus_tag="VYQ30_000221"
     CDS             20451..21173
                     /locus_tag="VYQ30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1965394.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            21173..21415
                     /gene="yidD"
                     /locus_tag="VYQ30_000222"
     CDS             21173..21415
                     /gene="yidD"
                     /locus_tag="VYQ30_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1965395.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(21640..22605)
                     /locus_tag="VYQ30_000223"
     CDS             complement(21640..22605)
                     /locus_tag="VYQ30_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1965396.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(22701..23453)
                     /locus_tag="VYQ30_000224"
     CDS             complement(22701..23453)
                     /locus_tag="VYQ30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1965397.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(23450..24406)
                     /locus_tag="VYQ30_000225"
     CDS             complement(23450..24406)
                     /locus_tag="VYQ30_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1965398.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(24396..25355)
                     /locus_tag="VYQ30_000226"
     CDS             complement(24396..25355)
                     /locus_tag="VYQ30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965399.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
BASE COUNT         7318 a         4870 c         5792 g         7422 t
ORIGIN      
        1 cccactacaa atattataga gccgattttt ttatacgatg ggcatgtcgt gcatctgagg
       61 tgtaagtcct ctgtagggat ccactaccgg tgaatacaat agcgctcccc aagcctgact
      121 atcgtgaggt agcggattaa aatggtctgg ggatagatcg ttttaagtct gacgcaggaa
      181 ataagaattg tcagagaaag caatagtgaa atcgtaggct atgaatagaa acgtgatagc
      241 gaggagcata tagtggataa ggtagcgaga aattttaaag tataaaagat agtcttaatc
      301 tatatatgta aatcacaaaa gtaattgaat tcgggataag gttggtgtga aaagacatag
      361 taaactgaat cttgaatact agaatgatac ttctacaata ttgttataat ttaatttgaa
      421 ttccaaaatc acattgttcc gtccgtattt cattttactg tatgtcttcc taaactccaa
      481 agtttctgcg tcaaatttcc tattttcact gtagtctttg acacttcgaa cgacaatttg
      541 aatcatatat tcaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat tctgaaaatt
      601 tgtttgattt ttaatggata atgtgatata atggttgtca atctataatt cacataaaca
      661 gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt tttattagca
      721 gcaggtgtac ttgcagcatg cagttcttca aaatcatctg attcatcagc ccctaaagct
      781 tatggctatg tttatacagc agacccagaa accttggact acctgatttc aagtaaaaat
      841 agtacaacag tagtgacttc aaatgggatt gatggtttat tcactaacga taattacggt
      901 aatcttgctc ctgcagttgc agaggattgg gaagtctcta aggatggttt gacctacact
      961 tataagattc gtaaaggggt taaatggttt acctctgatg gagaagaata tgcagaggtg
     1021 acggctaaag atttcgtgaa cggtttaaaa cacgcagcag ataaaaaatc agaagctatg
     1081 tatttagctg aaaattcggt taaaggcttg gcagattatc tatcaggaac ttcaacagat
     1141 ttttcaacag ttggtgtcaa ggcggttgat gattatacgt tacaatacac tttgaaccag
     1201 cctgaaccgt tctggaactc taagttgacc tattctattt tctggcctct gaatgaagaa
     1261 ttcgaaacat caaaaggaag cgattttgct aaaccaacag atccgacatc cttgctttat
     1321 aatggtccat tcttgttgaa agggttgact gcaaaatctt ctgtagagtt tgtaaaaaat
     1381 gagcaatatt gggataaaga aaatgtccac ctagatacta tcaatctagc ttactatgat
     1441 ggatcagatc aggagtcgct agagcgtaac ttcactagtg gagcttatag ttatgcccgt
     1501 ctttacccta ccagctccaa ctattctaag gttgcagaag aatacaagga caatatctat
     1561 tacacacaat caggctctgg gattgctggt ctgggtgtga atattgatcg ccaaagttac
     1621 aactatactt ctaaaactac agattcagag aaagtagcta ctaagaaggc attgcttaac
     1681 aaagatttcc gtcaagcctt gaattttgct cttgatcgct cagcttactc agctcaaatc
     1741 aatggtaaag atggagcagc tttagcagtt cgtaatttat ttgtaaaacc agactttgtt
     1801 tcagctggtg agaagacctt tggtgattta gtcgctgctc aacttcctgc ttatggtgat
     1861 gagtggaaag gtgtgaattt agctgatggg caggatggtt tattcaatgc tgacaaggcc
     1921 aaggcagagt ttgcgaaagc taagaaagct ttagaagcag acggcgttca gtttcctatt
     1981 catctggacg ttccagtaga ccaagcatca aaaaactaca tatctcgtat tcagtccttt
     2041 aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat tcaacaaatg
     2101 acaagtgatg aattccttaa tattacttac tatgctgcca atgcttcatc tgaggattgg
     2161 gatgtatcgg gaggagtttc atgggggcca gactatcaag acccatctac ttacctggat
     2221 attttaaaaa caactagcag tgaaactaca aaaacatatt taggatttga taatccaaat
     2281 agcccttcag tagttcaagt tggtttgaaa gaatacgata aattagttga tgaagctgcc
     2341 aaagagacaa gcgacttgaa tgtccgttat gaaaaatatg cagcggctca agcatggttg
     2401 acagatagtt cactctttat tcctgctatg gcttcttctg gtgcagcacc agtgctttca
     2461 cgaattgttc catttactgg agcttctgcg caaacaggct ctaaggggtc agatgtttac
     2521 ttcaaatatt tgaaattaca agataaagta gtgactaagg aagagtatga aaaagctcgt
     2581 gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga attggcaagt
     2641 catgtgaagt aaataatttc taatagaact ttctctccat gaggagaagg ttcttttggg
     2701 atttttaaag gaaataatat gaaaaaatat atttttatgc gtgttttgcg gtcattggtt
     2761 tcgattttct tagtaacgac tttgacctac acgattatct ataccttggt tcctcgaaaa
     2821 ttgattttca agcaggatcc taactataat aaaattgcga caacggctga taaacgtgat
     2881 aactatgaaa atactgtgtt tgagcgtatg ggctacattg agtattacga tactaaagag
     2941 ttgcaagaaa aggcaagtag catggattct tctgtaacag tagaagcaaa tgcgaccaat
     3001 aaagctattt atgaaaagta catcaatcaa ttaggtcatg gttggacttt gggagaattt
     3061 actgaaagtg gtcaattcta tgctactcgt gaaattccaa tttttgaacg tgtttttcac
     3121 ttctatgcta acttgattga cattgaccat acaaataaaa tccaagaccc tgaaaatcca
     3181 gacttgaaac gctaccttcg ttttgaaaat gatccagcta tcggatggtc attggtcggt
     3241 tcaggaacta aacataaata tctcttgtac tttaacagtc agttcccatt tgttcatcaa
     3301 aactttgtga acttgaattt aggtgactct tacccaacct atgctaatac accagttctt
     3361 caggttatta ctcaaggtca aggacaaacc aaaactgccc aagttcagtt cccaacaggt
     3421 aagaaaacgt cttctgtaaa tatttactca agaacctaca agtcacctag tcaggctgac
     3481 tctcgtgaag tagctagcta tgggaaagat gatccttata cagcgactga aagtaattac
     3541 caatatccat ctatgattgt cagctctgct attactggtt tgattggttt ggttcttgcc
     3601 tatgctcttg ccgtgccact tggttcagcc atggctcgtt tcaagaacac ttggattgat
     3661 agcctctcaa caggggcttt gaccttcttg cttgctcttc caacgattgc cttggtttac
     3721 atcgttcgat tgattggatc atctattgcc cttccagatt cattccctat cttgggagct
     3781 ggagattggc gttcttacgt tttaccagca gtcatccttg gtttgttggg tgctcctggt
     3841 acagccattt ggattcgtcg ttacatgatt gacttgcaat ctcaagactt tgttcgtttc
     3901 gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt taaaaatgcc
     3961 atggttccgc tggtttcagg aattcctggt gccattattg gggttatcgg tggtgcaacc
     4021 cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa tgttgattga ctctgtaaaa
     4081 gcatctaata actctatggt cgttggtctt gtcttcatct ttacatgtat ttctatcttc
     4141 tcacgtcttt tgggagatat ttggatgact attattgacc cacgtattaa attgactgag
     4201 aaagaggcaa ataatgtcta caatcgataa agaaaaattt cagtttgtaa aacgtgacga
     4261 ttttgcctct gaaactattg atgcgccagc atattcttac tggaaatcag tgtttaaaca
     4321 atttatgaag aaaaaatcaa ctgtagtcat gttgggaatc ttggtagcca tcattttgat
     4381 aagtttcatc tacccaatgt tttctaagtt tgatttcaat gatgtcagca aggtaaacga
     4441 ctttagtgtt cgttatatca agccaaatgc ggagcattgg ttcggtactg acagtaacgg
     4501 taaatcgctc tttgacggtg tctggttcgg agctcgtaac tccatcctca tttctgtgat
     4561 tgcgacagtg attaacttgg ttatcggtgt ttttgtcggt ggtatttggg gtatttcaaa
     4621 atcagttgac cgtgtcatga tggaagttta caacgtcatc tcaaacatcc cacctctttt
     4681 gattgttatt gtcttgactt actcaatcgg agctggattc tggaatctga tttttgccat
     4741 gagcgtaaca acatggattg gtattgcctt catgatccgt gtgcaaatct tgcgctatcg
     4801 tgacttggaa tacaacttgg cgtcacgtac tttgggaaca ccaaccttga agattgttgc
     4861 caaaaatatc atgcctcaat tggtatctgt tattgtgaca accatgactc aaatgcttcc
     4921 aagctttatc tcatacgaag ccttcttgtc tttcttcggt cttggattac cgattacagt
     4981 gccaagtttg ggtcgtttga tttcggatta ttcacaaaac gtaacaacca atgcttactt
     5041 gttctggatt ccattgacaa cccttgtctt ggtatccttg tcccttttcg tagttggtca
     5101 aaacttagcg gatgctagtg atccacgtac acatagatag gagtagaaat gacaaaagaa
     5161 aaaaatgtaa ttttgactgc tcgcgatatt gtcgtggaat ttgacgttcg tgacaaagta
     5221 ttgacagcca ttcgcggcgt ttcccttgaa ctagtcgaag gagaagtatt agccttggta
     5281 ggtgagtcag gatcaggtaa atctgttttg acaaagacct tcacaggtat gctcgaagaa
     5341 aatggtcgta ttgcccaagg tagtattgac taccgtggtc aggacttgac agctttatct
     5401 tctcacaagg attgggaaca aattcgtggt gctaagattg cgactatctt ccaggaccca
     5461 atgactagtt tggaccccat taaaacaatt ggtagtcaga ttacagaagt tattgtaaaa
     5521 caccaaggaa aaacagctaa agaagcgaaa gaattggcca ttgactacat gaataaggtt
     5581 ggcattccag acgcagatag acgttttaat gaatacccat tccaatattc tggaggaatg
     5641 cgtcaacgta tcgttattgc gattgccctt gcctgccgac ctgatgtctt gatctgtgat
     5701 gagccaacaa ctgccttgga tgtaactatt caagctcaga ttattgattt gctaaaatct
     5761 ttacaaaacg agtatcattt cacaacaatc tttattaccc acgaccttgg tgtggtggca
     5821 agtattgcgg ataaggtagc ggttatgtat gcaggagaaa tcgttgagta tggaacggtt
     5881 gaggaagtct tctatgaccc ttgccatcca tatacatgga gtctcttgtc tagcttgcct
     5941 cagcttgctg atgataaagg ggatctttac tcaatcccag gaacacctcc gtcactttat
     6001 actgacctga aaggggatgc ttttgccttg cgttctgact acgcaatgca gattgacttc
     6061 gaacaaaaag ctcctcaatt ctcagtatca gagacacatt gggctaaaac ttggcttctt
     6121 catgaggatg ctccaaaagt agaaaaacca gctgtgattg caaatctcca tgataagatc
     6181 cgtgaaaaaa tgggatttgc ccatctggct gactaggagg aaggaaatgt ctgaaaaatt
     6241 agtagaaatt aaagatttag aaatttcctt cggtgaagga agtaagaagt ttgtcgcggt
     6301 taaaaatgct aacttcttta tcaacaaggg agaaactttc tcgcttgtag gtgagtccgg
     6361 tagtgggaaa acaactattg gtcgtgctat catcggtcta aatgatacaa gtaatggaga
     6421 tatcattttt gatggtcaaa agattaatgg taagaaatcg cgtgaacaag ctgcggaatt
     6481 gattcgtcga atccagatga ttttccaaga ccctgccgca agtttgaatg aacgtgcgac
     6541 tgttgattat attatttctg aaggtcttta caatcaccgt ttatttaagg atgaagaaga
     6601 acgtaaagag aaagttcaaa atattatccg tgaagtaggt cttcttgctg agcacttgac
     6661 tcgttaccct catgaattct caggcggtca acgtcaacgt atcggtattg cccgtgcctt
     6721 ggtcatgcaa ccagactttg ttattgcaga tgagccaatt tcagccttgg acgtttctgt
     6781 acgtgcccaa gtcttgaact tgctcaaaaa attccaaaaa gagctcggcc tgacctatct
     6841 cttcatcgcc catgacttgt cggttgttcg ctttatttca gatcgtatcg cagttattta
     6901 caagggtgtt attgtagagg ttgcagaaac agaagaattg tttaacaatc caattcaccc
     6961 atatactcaa gccttgcttt cagcggtacc aatcccagat ccaatcttgg aacgtaagaa
     7021 ggtcttgaag gtttacgatc caagtcaaca cgactatgag actgataagc catctatggt
     7081 agaaatccgt ccaggtcact atgtttgggc gaaccaagcc gaattggcac gttatcaaaa
     7141 aggactaaac tagtaatggt tttataattt ccatgtcaat ttttatggaa attataaaac
     7201 tttttttgtt ggactgattt gaaaaacttt gaaagcttcc tgaaagaatt ttcgaaaaat
     7261 tttaaaaaat tctgaaatat tattgacagg tgatgaaaaa ggtggtagga taggaagcat
     7321 caaaaaagaa agcagaaaaa gaaatgaata agagacaagc cgtaactatg aatcaaaact
     7381 tttactttag ctactttaga tagtgtttgt agacatgtca ccatggatgc aaacagttca
     7441 agcaatttgt ttgtatctat gtggctaaga tttttgattg tggtccatat agatgaatat
     7501 ctaaatggac tagctaagtt gtaacttgtt aggttatttc aagtatccgt ttagatagta
     7561 tctaggcggt tttttgtttt atcttttctg agaaggagtt tcattatgaa agttgttcga
     7621 aaatcactag ccccctcttg gtagtggggc tcctcttgac ttctctgatc agtttgcatc
     7681 agttgaaggc agataagaaa aagatgtctt tcgtatcggt atttcgcaat ttattaccca
     7741 ccagtcctta tacgctacta gagaaggatt tgtggatgag ctggccaaac aaggctatgt
     7801 tgagggggaa aatattgaga ttgatttgca aaatgcacag ggagaacaaa gaaatctaaa
     7861 aacgatttcc cagcaactag cagaatccag cgatgtcgtt ctagccatcg caaggccttc
     7921 tgctcagagc ttggccaata cgacgcaaac gacaccagtt atcttttcag ctgtaacaga
     7981 cccagtcagt gccaagttgg ttgagtcaag agaacatcct gggggcaatg taactgggac
     8041 aagcgatcag tcatcagatg ccatttcaac ccaaatcaat ttgataaaga aagtgttgct
     8101 aaaggctaaa acgattggaa tcctctatac tcagagcgag cccaattcgg ttgtttaaaa
     8161 ggatgaagct aagcgacttc tgaaagaaaa aggctttacc gttgttgaaa aaacaatctt
     8221 ggacagtaat aacgtcaagg cggcagcaga gagtttgatg gcagaggtgg atatggtttt
     8281 tgtcccaact gataatatca tttcgtcaac catggaaaca gttaagcagg tttctattaa
     8341 acacaaggtt ccagtattcg gtggttcaac agaaatgatt gctgttggtg gcttgtataa
     8401 ctacggaacc aattatgaag aattgggaag acagacagct cgcatgctga ttcgtgtttt
     8461 aaaaggtgag gaaccagaaa atatagcagt tgagttgcct gaaaaactgg aattgcatac
     8521 taatcaagaa atggcagatg cattgggaat tgatattagt aagttagaag gcaaggaata
     8581 aggaggatta agatgaattt tgtgttatct agtttatcag aaggtttgtt gtggtcgatt
     8641 gtagcgattg gggtctactt gactttccgt attttggata ttgcggatat gactgctgag
     8701 ggagcctttc ctctgggggc agcagtcgtt gtatcacaga tacaggcagg ggcaaatcct
     8761 tggcttgcga ctttacttgc tttgctggca ggtatggtag caggtcttgt atcaggaatg
     8821 ctccacacca agatgaaaat cccagctctc ttgacaggga ttgtgacctt gacagggctc
     8881 tattcaatca atattaaaat catgggcagt gtatccaatc tttccttggg agattctgca
     8941 accgtcttta aacagttggc gagcttgggt ttgacaaatg aaggaactgt tttctcactt
     9001 agtttggcct gttttttact tgtttgtttg gtcttgaccc ttttgatgaa aacagagatt
     9061 ggcttggtct tgcgttcgac tggggataat atcccgatga gtgaggccaa tggagtcaat
     9121 gtagacacca tgaagattgt tggttacatg atttcaaacg gtttgattgc tctatgtggt
     9181 tccttgtttg cccaaaatga tggattttca gatgtgactt ctgggacagg aaccattgtt
     9241 gttggtttga gctcggtgat tattgcggaa gtcttgatac acgacttgac cattggaggc
     9301 cgcttattat ccatcggaat cggtgctatt gtttaccgtt tgattatttt aaatatctat
     9361 gaaattccaa atctagatca aaacttggta cgtctcttta atgcaatctt gctcgctttg
     9421 gttttatttg caccagaatt gcaaaagaga ttaaagattc gtggtctgaa attgagaaat
     9481 gaataggagg agaaagttat gacaagtttg ttaacacttg aaaatattca caagacattt
     9541 gaagcaggga cggtcaatga gaaccatgtc ctcaaaggat tagatttaga ggttgaagag
     9601 ggagatttta tctctgtgat tggtggaaat ggagcaggaa aatccacttt gatgaatatc
     9661 ttggctggca atctatcggt ggacgaaggg gaccttctac tggcagggaa atccattaaa
     9721 aatctgagtg tcagaaagag agccaaggat attgcccgtg tttttcaaga tcctaagatg
     9781 ggaacagctt ctcgtttgac gattgaggag aatatggcta ttgccttgag acgcgggcag
     9841 aagagagggc ttggttgggg cgtgaaggag aaggatagaa tccagttcca agaggccttg
     9901 aaggagttga atattggtct tgaaaaccgc ttaaaagtag atactcaata tctctcagga
     9961 ggacaaagac aggctttgac tctagtcatg acagatctga tgaaaccaaa acttttactt
    10021 ttggatgaac atactgcggc acttgacccg aaaaccagtc aaatggtgat gaacttgacg
    10081 caaaagattg tggagcacca tcagtggacg actttgatga ttacccatga tatgaatcat
    10141 gcgattgagt acggcaatcg tctcattatg ctctatcaag gcaagatagt ggtggatgtc
    10201 aagggagaag agaaaaagca tctgacagtt gaagacctta tgcatctctt ccagaaaaat
    10261 agtggccaaa gtctggtcag tgatgaatta gttttgggat aaagaaaaag gctgagatct
    10321 ggtgtctcag tcttttattt cttgcgtttt gcaaaatcta caaatctaaa tttctctaac
    10381 ttatggcgac tttcggtaaa ctgaaattgg cgtccgtctt ggagatagac ttttgattta
    10441 atcgaaacga cataagggtc tttgccaatg tccatgagaa tcttgtctcg gtcgcttgag
    10501 tggtcaatgg tgatttcctt ctgagcataa tcaataagga gtttgaggcc attttctata
    10561 taagaataga tagactgctc agcaatttgg cgagtgagat ttggaattag ttccatatcc
    10621 agatagtccg tatccagaac ggataccaga tcatccacca cacgctggcg gaccaccttc
    10681 caaaccatcc gaaactctgg gaaaccggtt atcagtgagg attttttatc aataataatc
    10741 ttgtccagac tgaccacgtt ggttttagag cgcagtccaa gttctttaac tagttcttgg
    10801 tagctggtta ggttggatac agggaaattg acggtttctt ctttgacgac ttgagaacct
    10861 tgccctctta tctttttgat caatccttcc tcttggagga gagacagggc ctttcggacg
    10921 gtatcacgac tcacttgata ttgctccata aggtcgtgct cgctaggaag gaaatctccg
    10981 acagcgtaag tctcgttttg aatggtttct tggatttgct taaataattg ttggtatttc
    11041 ttcattttgc ctgcttaaat aggtttttag tctagtttaa tgaacacctg tatctatttt
    11101 accatacaag tgctagaaaa tttacaaaaa aacacccttt ttataccaaa caagttgcag
    11161 acaagtttgg tatcgtttac aatatactta tcaaatcaaa cttgctttga caagtataag
    11221 gagaatcaaa tgggaaaatt tgaacaagaa gccaaagatc tgcttcaggc aatcggaggc
    11281 aaagaaaatg tgactgccgt aactcactgt gcgacacgga tgcggtttgt tttaggagat
    11341 gataagaagg ctaatgttaa agctatcgag tcaattccag ctgttaaagg aacctttaca
    11401 aatgcaggtc aatttcaggt aatcattgga aatgacgtgc ccatctttta taatgatttt
    11461 acagccgttt caggtattga gggtgtttcc aaagaagcag ccaagtctgc agctaagagt
    11521 aatcaaaacg tggtccaacg tgttatgacc actctggcgg aaatctttac tccgattatt
    11581 ccagccttga ttgttggtgg attgatcctc ggtttccgta atgtcttgga aggtgtccat
    11641 tggtcgatgt tggatggcaa gaccatcaca gaatcctctc agttttgggc aggggttaat
    11701 cacttcctct ggttgcctgg tgaagctatc ttccagttct taccagtagg gattacttgg
    11761 tctgtttctc gtaagatggg aaccagccaa attttgggaa ttgttctcgg aatctgtttg
    11821 gtatcgcctc agttgctcaa tgcctatgcg gttgcttcaa cgccagcagc tgatatcgcg
    11881 gcaaactggg tttggaattt tggctatttt actgttaatc gtatcggtta ccaagcccaa
    11941 gttatcccag ccttgcttgc aggtttgagt ctgtcttatc ttgaaatctt ctggcgcaag
    12001 catatcccag aagtcatttc tatgattttt gtacctttct tgtcattgat tccagccttg
    12061 attttggctc atactgtttt gggaccaatc ggttggacaa ttggacaagg actttcatca
    12121 gttgtcttgg caggtttaac tggtccagtt aaatggctct tcggtgcaat ttttggtgcc
    12181 ctctacgctc catttgtcat cacaggtctg caccatatga ccaatgccat tgatacacaa
    12241 ttgattgcgg atgctggtgg cactgcccta tggccaatga ttgctctttc taatattgct
    12301 caaggctcag ccgtgtttgc ctattatttc atgcatcgcc atgatgagcg tgaggctcag
    12361 gtttcacttc ctgcaaccat ttcagcctat ctcggtgtta cagaaccagc tctttttggg
    12421 gttaacgtaa aatatattta tccatttgtt gctgggatga ctggttcagc ccttgcaggc
    12481 atgttatccg ttacttttaa tgtaactgcg gcttctattg gtatcggtgg tttgccaggt
    12541 attctctcta ttcaacctca atacatgctg ccatttgcag gaactatgct agttgcgatt
    12601 gttgttccaa tgctcttgac tttcttcttc cgcaaggctg gtctctttac aaaaacagag
    12661 ggcgatacga acttgcaggc agaattcgtt gctcaagaag aagcagaatt tgtgaaccat
    12721 gaaccagtag aacttacttc ggtagaaatt atcagcccac taactggcca agtgaaagaa
    12781 ttgagtcaag cgacggatcc tatttttgca tcaggtgtca tggggcaagg tctggtcatt
    12841 gaaccaagcc aaggtgagtt gacctctcca gttaatggga cagtgacggt tcttttccct
    12901 accaagcatg ccatcggcat tgtctctgac gagggagttg aattgctcat ccacatcggt
    12961 atggatacag taggtcttga tggcaaaggt tttgaaagtc ttgtagtcca aggagatcac
    13021 gttacagttg gtcagcaact gattcgtttt gatatggatg tcattaaggc tgcaggtctg
    13081 gtgacagaaa ctcctgttat catcaccaac caagatgctt atacagcgac tattcccgga
    13141 acttatccga caacgatcca agctggagca tctctcatgg tcgctacacg aatctaatac
    13201 tcttcgaaaa tctcttcaaa ccacgtcagc tctatctgca acctcaaaat agtgttttga
    13261 gcagcttcct agtttgttct ttgattttca ttgagtataa ttgaaatagc acaaataggg
    13321 acggaagact gcttcctccc ttttatcaga aaaggagaaa cagatgacac ttgataaagg
    13381 aaaagtagtc tatcaaatct atccaaaatc ttacaaagac accactgaaa acggtattgg
    13441 ggatttccgt gggattatcg aaaaaattcc ctatttagcc aaactaggtg tggatatggt
    13501 ctggctcaat ccattctatc caagccctca acgggataat ggttacgata tttcagatta
    13561 tatggcagtg gatcctcttt ttggtgatat ggctgatttt gaggaaatgg tgtgtgtcgg
    13621 taaagagcac aagattgact ttatgctgga catggtactt aatcattgtt cgacagagca
    13681 tgaatggttt cagaaagccc tagctggtga taagtattat caagactttt tcttcatcca
    13741 agaccaacca acagattggc agtctaagtt tggtggttct gcatgggcgc ctttcgggga
    13801 tactgggaaa tactatcttc acctatttga tgagacccag gctgacctta actggcgcaa
    13861 ttccaatgtc cgtaaggagc ttttcaaggt tgttaatttc tggcgcgaca agggtgtcaa
    13921 aggtttccga tttgatgtga tcaatttgat cggtaaggac gaggtttcag tggactgtcc
    13981 tgaaaatgaa gggaagccag cttatacaga caaacccatc gttcataact atttgcgtat
    14041 gatgaaccaa gccacttttg gatctgacga tagctttatg acagttgggg aaatgtcttc
    14101 taccaccatg gaaaactgtg tcctctattc gtcgcctgat cgtcaggaat tatccatgac
    14161 ctttaatttt catcacctca aggtggacta taaggatgga caaaagtgga ccttggctcc
    14221 ctttgatttt gaagagttga agagtcttta ccatagttgg ggtaaggaaa tgagtgataa
    14281 agacggttgg agtgccctct tttggaacaa tcacgatcaa ccacgtgcct tgaaccgttt
    14341 tgtcgatatt caaaacttcc gcaaggaagg agccaccatg ctagcagcca gcattcacct
    14401 gtcacgtggg acaccttata tctacatggg tgaggaaatc gggatgattg acccagacta
    14461 tgattctatg gctgattatg tggatgtcga atcgcttaat gcctatcaga tgctcttaga
    14521 agaaggaaag agtcagcaag aagccttcca gattattcaa gctaagtcgc gtgataattc
    14581 acgcattccc atgcagtggg atgcttcgga aaatgcagga ttttcaacag gcactccttg
    14641 gttgaaagct ggtaaatcct ataaagacat caatgtagaa aatgaaatcc agggtccgat
    14701 ttttaccttc tatcaagact tgattcgact gcgtaaggaa atgccaatca tttcagaagg
    14761 aagttacaaa ccagcctttg aagatagcaa acaagtctac gcttttgaac gccagtttga
    14821 ggatcaaaag ttactagtgc tcaataactt ttatgctaaa gaagtggaaa tcgacttgcc
    14881 agcagtctac caaaatggac agattttgat atcaaactat gaagatgcag aagtgtctga
    14941 gaaaattcta ctcaaaccct atcaaacact ggctatttat gtaaattaat actcttcgca
    15001 aatctcttca aaccgtgtca gttttatctg caacctcaaa gcggtgcttt gagcaacctg
    15061 tggctagctt cctagtttgc tctttgattt tcattgagta taaacttagc aaggagacct
    15121 ctttttcaga aaaacagggt tctttttgct gtctattcca tccttgaaag tgtagaacaa
    15181 actggtcagg gtggctagtt tgtggtataa tgaaagggat tcaaaaagaa acaggagaaa
    15241 aatgatggat ttacttttag caattgtatt gattgtgcta gcttttctag gaggagctct
    15301 tggaggaatg tacttggttc gtaagcaaat cgaaaaagaa tttgctgaca acccacgttt
    15361 gaatgctgaa gcagttcgta ctcttttgag tgcaaatggt caaaaaccaa gcgaagctaa
    15421 ggtacaacaa gtttaccacc aaatcatccg ccaacaaaag gcagcccttg ctaacaataa
    15481 aaagaaaaaa taaataagga aaagtctggg atgaaagttc cagacttctc actatgttgg
    15541 ttatggttta gggatgagac tttttccttg cattctattg atatttgatt atgattaaga
    15601 gagaggtagt tgacattcta tcaagctgtc ggtcagttcg ttcagtacaa ggaatcataa
    15661 tgaacgatca atcagaaaaa agactagaaa gaagactgta tggataatcg accaattggt
    15721 tttttggatt cgggtgtcgg gggcttgacc gttgtgcgcg agctcatgcg ccagcttccc
    15781 catgaagaaa tcgtctatat tggagattcg gcgcgggcgc cctatggccc ccgtcctgct
    15841 gagcaaattc gtgaatatac ttggcagctg gtcaactttc tcttgaccaa ggatgtcaaa
    15901 atgattgtca ttgcttgtaa cactgcgact gcggtcgtct gggaagaaat caaggctcaa
    15961 ctagatattc ctgtcttggg tgtaattttg ccaggagctt cggcagccat caagtccagt
    16021 caaggtggga aaatcggagt gattggaacg cccatgacgg tacaatcaga catataccgt
    16081 cagaaaatcc atgatctgga tcccgactta caggtggaga gcttggcctg tcccaagttt
    16141 gctcccttgg ttgagtcagg tgccctgtca accagtgtta ccaagaaggt ggtctatgaa
    16201 accctgcgtc ccttggttgg aaaggtggat agcctgattt tgggctgtac tcattatcca
    16261 ctccttcgcc ctattatcca aaatgtgatg gggccaaagg ttcagctcat cgatagtggg
    16321 gcagagtgcg tacgggatat ctcagtctta ctcaattatt ttgaaatcaa tcgtggtcgc
    16381 gatgctggac cactccatca ccgtttttac acaacagcca gtagccaaag ttttgcacaa
    16441 attggtgaag aatggctgga aaaagagatt catgtggagc atgtagaatt atgacaaata
    16501 aaatttatga atataaggat gaccaggact ggtatgttgg gtcttatagt atttttggtg
    16561 gcgttaacag tttgagcgac tataagacag attttcctct gtttgaattc tccaaaatat
    16621 ttggagatga agagtatggt ttcccgcttt cagttactgt tttacgctat ggttctacct
    16681 accgtttgtt ctcctttgtg gtagacatgc ttaatcaaga aatgggacga aacttggaag
    16741 ttattcaacg tcatggggcc ctgctcttgg ttgaaaatgg gcaactcttg tatgtagaat
    16801 tgcctaaaga aggggtcaat gttcatgatt tctttgagac aagcaaggtc agagaaacct
    16861 tgttgattgc gactcgtaac gaaggtaaaa ccaaggaatt ccgagctatc tttgataagt
    16921 taggctacga tgtggaaaat cttaatgact accctgacct gcctgaagta gcagaaacag
    16981 gtatgacctt tgaggaaaat gcccgcctta aggcagaaac catttctcaa ttaacgggca
    17041 agatggtttt ggcagatgat tctggtctca aagtcgatgt ccttggtggc ttaccaggcg
    17101 tctggtcagc tcgtttcgca ggtgtgggag caactgaccg tgaaaataat gccaaactct
    17161 tgcacgaatt ggccatggtc tttgaactca aggaccgctc ggctcagttc cacacaaccc
    17221 tagtcgtagc cagcccaaat aaggaaagtt tagttgttga agcagactgg tcaggttata
    17281 ttaactttga acctaagggt gaaaatggct ttggctatga tcccctcttc cttgtaggag
    17341 aaacaggtga gtcatcagct gaattaaccc tggaagaaaa aaatagtcaa tctcaccgtg
    17401 ccttggccgt taagaaactt ttggaggtat ttccatcatg gcaaagcaaa ccatcattgt
    17461 aatgagcgat tcccatggcg atagcttgat tgtggaagaa gtccgtgatc gctatgtggg
    17521 caaagtcgat gccgtttttc ataacggcga ttctgaacta cgtccggatt ctccactttg
    17581 ggagggcatc cgcgttgtta aagggaacat ggacttctac gccggctacc cagaacgtct
    17641 ggtgactgag cttggttcga ccaagattat ccaaactcat ggtcacttgt ttgacatcaa
    17701 tttcaacttt caaaagttgg actactgggc tcaggaggaa gaggccgcta tctgcctcta
    17761 tggtcacttg catgtgccaa gtgcttggat ggaaggcaag atcctctttc taaatccagg
    17821 ttctatcagc caaccacgag gtaccatcag agaatgtctc tatgctcgtg tggagattga
    17881 tgatagttac ttcaaagtgg actttttgac acgagatcac gaggtgtatc caggtttgtc
    17941 caaggagttt agccgatgat tgccaaggag tttgagactt tcttgttggg gcaggaggaa
    18001 acttttttga cccctgctaa aaatctagct gtgttgattg atacccacaa tgcggatcat
    18061 gcgaccctct tgctcagtca gatgacctat acccgtgttc ccgttgtgac agatgaaaaa
    18121 cagtttgttg ggacgattgg actcagagat attatggctt atcagatgga gcatgacttg
    18181 agccaagaaa tcatggcgga tacggatatc gttcatatga caaaaacgga cgtagcggtt
    18241 gtttcgcctg atttcaccat tacggaggtc ttgcacaagc tagtagatga gtccttctta
    18301 ccggttgtgg atgcagaggg tattttccaa gggattatta cgcgcaagtc catcctcaag
    18361 gccgttaatg ccctcttgca tgactttagt aaggaatatg agattcgatg ccaatgagag
    18421 acaggatttc agccttttta gaggaaaagc agggcttgtc tgtcaattcc aagcagtcct
    18481 ataagtatga tttggagcaa tttttagaca tggtaggtga gcggatttct gagaccagtc
    18541 tcaagattta ccaagcccag ctagccaatc taaaaatcag cgcccagaag cgaaagattt
    18601 cggcctgtaa ccaatttctc tactttctct atcaaaaagg agaggtggac agcttttacc
    18661 gcttggaatt agccaaacaa gctgaaaaga agacggaaaa gccagagatt ctatacctag
    18721 actctttttg gcaggaaagc gaccatccag agggccgctt gctagcgctc ttaatcctag
    18781 aaatggggct cttgcccagt gagattttag ccatcaaggt tgcggacatc aatctggatt
    18841 ttcaggtgtt gcgaatcagc aaggcttccc aacagaggat tgtcaccatt cccacggcct
    18901 tgctttcaga attggaaccc ttgatggggc agacctatct ttttgaaaga ggagagaaac
    18961 cctattctcg tcagtgggcc tttcgtcagt tagaatcttt tgtcaaggag aaaggttttc
    19021 catccttatc agctcaagtc ttacgtgaac agtttattct aagacaaata gaaaacaagg
    19081 tcgatttgta cgaaattgca aaaaaattag gattaaaaac agtcctgacc ttagaaaaat
    19141 atagataatg gatattaaat taaaagattt tgaaggaccc ctggacttgc tcttgcatct
    19201 ggtttctaag taccagatgg atatctacga tgtgcccatt acggaagtca tcgaacagta
    19261 tctagcctat gtctcaaccc tgcaggccat gcgtctggaa gtgacgggtg agtacatggt
    19321 catggctagt cagctcatgc tgattaagag tcgtaaactc cttccgaagg tagcagaagt
    19381 gacagacttg ggggatgacc tggagcagga cctcctctct caaatcgaag aatatcgcaa
    19441 gttcaagctc ttgggtgagc acttggaagc caagcaccaa gaacgggccc agtattattc
    19501 caaagcgccg acagagttga tttacgaaga tgcggagctt gtgcatgaca agacgaccat
    19561 tgacctcttt ttggcttttt caaatatcct agccaagaaa aaagaggagt ttgcacaaaa
    19621 tcacacgacg atcttgcggg atgagtataa gattgaggac atgatgatta tcgtgaaaga
    19681 gtccttgatt ggacgagatc aattgcgctt gcaggatttg ttcaaggaag cccagaatgt
    19741 ccaagaggtc atcaccctct ttttggcaac cctagagtta atcaaaaccc aggagctgat
    19801 cctcgtgcaa gaggagagtt tcggagatat ctatctcatg gaaaagaagg aagaaagtca
    19861 agtgcctcaa agctagactt gatagagagg aaagatgagt actttagcaa aaatagaagc
    19921 gctcttgttt gtagcgggtg aagatgggat tcgggtccgc cagttagctg aactcctctc
    19981 tctgccaccg acaggcatcc agcagagttt aggaaaatta gcccagaagt atgaaaagga
    20041 cccagattcc agtttggctt tgattgagac aagtggtgct tatagattgg tgaccaagcc
    20101 tcaatttgca gagattttga aggaatactc taaggcgcct atcaaccaga gcttgtctcg
    20161 ggctgccctt gagaccttgt ccattattgc ctacaaacag ccgattacgc ggatagaaat
    20221 tgatgccatc cgtggggtta actcgagtgg agccttggca aagttgcagg cttttgacct
    20281 gataaaggaa gacgggaaaa aggaagtatt ggggcgcccc aacctctatg tgactacgga
    20341 ttatttccta gattacatgg ggataaacca tttagaagaa ttaccagtga ttgatgagct
    20401 tgagattcaa gcccaagaaa gccaattatt tggtgaaagg atagaagaag atgagaatca
    20461 ataagtatat tgcccacgca ggtgtggcca gtaggagaaa agcagaagag ctgatcaagc
    20521 aaggtttggt gacagttaac ggacaagtgg tgcgtgaact agcaaccact atcaagtcag
    20581 gcgacaaggt cgaagttgaa ggtcaaccta tctacaacga agaaaaggtc tattatctgc
    20641 ttaacaaacc acgcggtgtc atttccagtg taacagatga caagggtcgc aagacggttg
    20701 tcgacctctt gcccaatgtc aaagagcgta tttaccctgt gggtcgtttg gactgggata
    20761 catcaggagt cttgattttg accaatgatg gggactttac agacgagatg attcaccctc
    20821 gtaatgagat tgacaaggtt tatgtcgcgc gtgttaaagg tgtggccaat aaggacaatc
    20881 tccgcccctt gacccgtggt cttgagattg atggtaagaa aaccaagcca gctgtttatg
    20941 aaattctcaa agtggaccca gtcaaaaatc gctctgtggt gcagttgacc atccatgaag
    21001 ggcgtaacca tcaggttaaa aagatgtttg aagctgttgg tctccaagta gataagttgt
    21061 ctcggactcg tttcggacac ctagacttga caggactccg tccaggagaa tcccgtcgtc
    21121 ttaataaaaa agaaatcagc caactacaca ccatggctgt aactaaaaaa taatgaaacg
    21181 aattttaata gcgcctgtgc gcttttacca acgttttatc tcaccagtct ttccaccctc
    21241 ttgtcgcttt gagctgactt gttccaacta catgattcag gctattgaaa aacatggttt
    21301 taagggggta ttgatgggct tggctcggat tttacgttgt catccctggt cgaaaacagg
    21361 taaggatccc gttccagacc acttttccct taaacgaaat caagaagggg aatgaggtgg
    21421 ggtaaataga tttcaaaatg ataaaaacgc atcctatcag gtttgagtga acttgatagg
    21481 atgcgtttta gaatgtcaaa attttatact cttagaaaat ctcttcaaac cgcatcagct
    21541 ttcatctgca acctcaaaac agtgttttga gcaacctgcg gctagtttcc tagtttgctc
    21601 tttgattttc attgagtatt aaattgagtt tgaagtggct tatttcaaag ctttttgtat
    21661 gtcttcaatc atgagttttg ttgattcaag tccgcctccg cttagatacc agaggtctgg
    21721 tgttagttgg ataatcttac catttttagc agcaggtgtt tcagcgataa gggcattttc
    21781 taggacaccg tcgttgctag agttgtcccc accgatggca agggtacggt tgatgacaaa
    21841 gaggatgtca gggttgattt ctttgacact ttcaaagctg acttcttgtc cgtggcgtga
    21901 gtcttcaaat gttgtatcag ttggtttgaa tttcaaggtt tggtacaaga aagagaaacg
    21961 agatttggca ccaaaggctg ccatttttcc ttcattaagg aggatcgcaa gggctttttt
    22021 gtcagagctt tcatttttag tagcgacttc ttggatgctc ttgtctagct tggccaattc
    22081 ttccttggct ttctgtgtac cagtttcgcc gaaggcactt gctaaggatt cgatattagc
    22141 cttggtagaa gtccagtagt cgtccttgct tgcttggaag agaacggttg gggcgatttc
    22201 tttgaatttg tctacgaatt tttgtgtacg tggtgaagcg ataatcaaat caggctcaag
    22261 ggcggcgata gcttctaaat caggttcttt catagaacca acatttttga cagttcccac
    22321 taggtctttt agataagtcg gaacagtttt tgtaggcatt ccgacgatat ttttttcaaa
    22381 tcctaaagcg cgaatagtat ctgcagcgcc gaggtcaaag gtcacaatct tttcaggaac
    22441 tttggaaagt ttgacctcgt ccagtgaact tttaatggtt atctctgttg gagcagagct
    22501 actggtctct gtctgactag tgcttgagtt tgtactacat gcaccaagta ggagcaagaa
    22561 gctggccact agggcagtga aataaagttt aagggatgtt ttcataattt ctccttttta
    22621 aaatgtgata acgatttagg gagtctctta atcttattga ctaagagact gaaggttctc
    22681 taacttgagc ttttatgtta ctagctatag atacagatct ttttgttatt gatatcagct
    22741 agcgtgatgg gaatctcata aagttgactg agcaggtcag cctgcatgat ttgatcggtt
    22801 cttcccttgc taaagacctg gccgtccttg aaggcgacaa tttcatctgc atactgactg
    22861 gccatgttga tatcgtggag gacgatgata atggtcttgc cgagttcctc caccagtcgt
    22921 cgaagaatct gcatcatgct gacgctttgc ttgatatcga gattgttgag tggttcgtcc
    22981 agcaagataa agtccgtatc ctgggccagt accatagcga taaagacccg ctggagttgc
    23041 ccccctgaca ggctattgat gtagcggtct tttaagttgg tcagttccaa atagttcaga
    23101 gtttctcgga ttttttccca gtcttctgat ctaagtcgac ctcggctgta gggaaaacgt
    23161 ccaaaactga ccagttcttc aacagtcaat ttggcttggt aattgatttt ctgttttagg
    23221 atggttagtt cttgggccag ttcttgcgaa ttccagctct cgatttcacg tcctttgata
    23281 ctgagaactc cctgatcttt cttggttagt ctgctcatga tggagaggag agtcgatttt
    23341 ccagcaccat ttggaccaat aaaggctgtc agtttttgag gactgacttc aagcgaaatg
    23401 ccttgcaaaa tatcctgttt ttgaatggat ttgtcaatgt tttccagttt cactgacgag
    23461 acctcctata tagtaagata aagaataaga agccacccac actctcaatg atcatactga
    23521 tacgaatttc cagtgcaaag actcgttcaa tcaaggcttg ccccaaggtt aagctaataa
    23581 atccaaccag aatggccact ataaagagta acttgtgctg atagtctttg acaatcaggt
    23641 aggtgaggtt ggccagcata aagccgaaga aggccatagg tcctaccaag gcagtggccg
    23701 ttgaggtcaa aagcacgatt ccccagagga gctctttctg ttctttttca acatcgagtc
    23761 ccaatatctg agccgtttct ctttgcaggt gcaagacatc tagaacgact gcttttcgaa
    23821 agaaaaagat tgtcaaagcg aggatgatca gagaaccgat ggctaggatg gaagtgttga
    23881 gatgttgaaa ggaggcaaaa agactatttt gcagtttatc gtattcgttt ggatccatta
    23941 ggacttgaag gaaggtactg atatttcgaa agagacttct gagcgctaga cagatcagca
    24001 ggatgaagac caggtcttgc ttcatcagtg tcttcaagta accttgtaag gcgagaaaga
    24061 agagggactg gacaagaagt aagactagga attctaagat aggggatttg ccaagttgaa
    24121 gaaacttgct ttcaaaaacc agtagtaggg tttgtagtag gacgtagaag gattcaattc
    24181 ccaaaatact aggcgtcagg aagcgatttt ccgtcagggt ttgaaaacta atggtcgaaa
    24241 tcccagtcgc gatggctacc aagagataaa cgatgatctt ttgggaacgc aacttccaag
    24301 caaaggctga caagtgagtg atgggccaaa agtagagaag acaagctccg atggcaagaa
    24361 taatgagaat ccagaagagc ttggtatgtt tgcttttagt ctgcatcttt tcgtccccct
    24421 ctccagagaa gtaggataaa gacgagacta ccgatgattc ctagcaagag actgacagac
    24481 aactcatagg gcctaatcag aactcgggat aggatatcgc aagccagaac tagattggca
    24541 ccaaccagtg cgaccatgag tttggtttga cttagattat ctccatagcg cttgcgaaca
    24601 agattgggaa cgataactcc gagaaatggt aagccaccca cggtaatcat ggtgacgctt
    24661 gtcgttagcg ccaccagaaa gagggctagt ttttcaagta gggagtagga aatccccaaa
    24721 ctctcgctgg tttctttccc tagattcatg atggtgaagg tttgggataa tttccaaacg
    24781 gttatcagga tgatgaggcc taagaagagc cactcatact gatgggtctg aatcatggag
    24841 aaggagccct gggtccaggc agtcatactc tgaaccagat tgaaacgata ggcgataact
    24901 tctgtgactg agccgataat cccgctatag atgatcccaa tcagaggcaa catccacctt
    24961 tcctttacag taaaaatggt cataaaggct aggaagaaga gggtgaatac gatggatgaa
    25021 acaaaagcga agagcatctt ttgggtcaga ctagccgatg gaaagacaaa gaggctcagc
    25081 accattccca gtttggcggc ttcagtcgtt ccaactgtac tcggtgcagc aaactgattt
    25141 tgggtaatag tctgcatgag aaggcctgcc atactcatac tagaggcagt caggagaata
    25201 ctgatagttc ttgggagacg ggactcttga aagaggagcc aggtctgctg gtcgaaatca
    25261 aatagctttc cccatgaaaa atcactggtc ccaatgctaa tagagagaaa gactaggagt
    25321 agaagtaagc caattaaata atgagaaagt ttcatacccc gtcctttcat gtagatttgg
    25381 tatcgaaaga tacctgcgga ta
//
LOCUS       DAVMEF010000008        25176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMEF010000008 DAVMEF010000000
VERSION     DAVMEF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.008"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            63..710
                     /locus_tag="VYQ30_000227"
     CDS             63..710
                     /locus_tag="VYQ30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1965400.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(699..1503)
                     /locus_tag="VYQ30_000228"
     CDS             complement(join(699..1133,1135..1503))
                     /locus_tag="VYQ30_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1965401.1"
                     /translation="MNDEASKQLTDARFKRFVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKFHAMKTQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTSRKSSKLKPLTVEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            1498..1752
                     /locus_tag="VYQ30_000229"
     CDS             1498..1752
                     /locus_tag="VYQ30_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1965402.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1998..3332
                     /gene="trmFO"
                     /locus_tag="VYQ30_000230"
     CDS             1998..3332
                     /gene="trmFO"
                     /locus_tag="VYQ30_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1965403.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3416..4153
                     /locus_tag="VYQ30_000231"
     CDS             3416..4153
                     /locus_tag="VYQ30_000231"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1965404.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            4162..4719
                     /gene="frr"
                     /locus_tag="VYQ30_000232"
     CDS             4162..4719
                     /gene="frr"
                     /locus_tag="VYQ30_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1965405.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4779..5633
                     /gene="cvfB"
                     /locus_tag="VYQ30_000233"
     CDS             4779..5633
                     /gene="cvfB"
                     /locus_tag="VYQ30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1965406.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5642..5857
                     /locus_tag="VYQ30_000234"
     CDS             5642..5857
                     /locus_tag="VYQ30_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1965407.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5943..6947
                     /locus_tag="VYQ30_000235"
     CDS             5943..6947
                     /locus_tag="VYQ30_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1965408.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEPAPVKKEESEELESQPLSEG
                     "
     gene            7055..7624
                     /locus_tag="VYQ30_000236"
     CDS             7055..7624
                     /locus_tag="VYQ30_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965409.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLVNPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTETNRAVIELAFEKIGMNKLVALYDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            7692..8342
                     /locus_tag="VYQ30_000237"
     CDS             7692..8342
                     /locus_tag="VYQ30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1965410.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKQVSGIGAKTIEKLKDYVTVD"
     gene            8326..10566
                     /locus_tag="VYQ30_000238"
     CDS             8326..10566
                     /locus_tag="VYQ30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1965411.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTVKVNGDSL
                     SFRGKADGRIFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQEIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLWDITGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKQYPYLEVDVLKAAQHGSKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            10745..10933
                     /locus_tag="VYQ30_000239"
     CDS             10745..10933
                     /locus_tag="VYQ30_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965412.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            10965..11552
                     /locus_tag="VYQ30_000240"
     CDS             10965..11552
                     /locus_tag="VYQ30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965413.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            11556..12737
                     /locus_tag="VYQ30_000241"
     CDS             11556..12737
                     /locus_tag="VYQ30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965414.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    12897..13028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13044..13574
                     /gene="infC"
                     /locus_tag="VYQ30_000242"
     CDS             13044..13574
                     /gene="infC"
                     /locus_tag="VYQ30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1965415.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            13607..13807
                     /gene="rpmI"
                     /locus_tag="VYQ30_000243"
     CDS             13607..13807
                     /gene="rpmI"
                     /locus_tag="VYQ30_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1965416.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            13859..14218
                     /gene="rplT"
                     /locus_tag="VYQ30_000244"
     CDS             13859..14218
                     /gene="rplT"
                     /locus_tag="VYQ30_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1965417.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            14276..14656
                     /locus_tag="VYQ30_000245"
     CDS             14276..14656
                     /locus_tag="VYQ30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1965418.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            14906..15706
                     /locus_tag="VYQ30_000246"
     CDS             14906..15706
                     /locus_tag="VYQ30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1965419.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            15717..16655
                     /locus_tag="VYQ30_000247"
     CDS             15717..16655
                     /locus_tag="VYQ30_000247"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1965420.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            16697..18730
                     /locus_tag="VYQ30_000248"
     CDS             16697..18730
                     /locus_tag="VYQ30_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1965421.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWMWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(18791..20446)
                     /gene="pavA"
                     /locus_tag="VYQ30_000249"
     CDS             complement(18791..20446)
                     /gene="pavA"
                     /locus_tag="VYQ30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1965422.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            20551..21048
                     /gene="ybeY"
                     /locus_tag="VYQ30_000250"
     CDS             20551..21048
                     /gene="ybeY"
                     /locus_tag="VYQ30_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1965423.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21029..21424
                     /locus_tag="VYQ30_000251"
     CDS             21029..21424
                     /locus_tag="VYQ30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1965424.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            21441..22340
                     /gene="era"
                     /locus_tag="VYQ30_000252"
     CDS             21441..22340
                     /gene="era"
                     /locus_tag="VYQ30_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1965425.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            22389..23213
                     /gene="mutM"
                     /locus_tag="VYQ30_000253"
     CDS             22389..23213
                     /gene="mutM"
                     /locus_tag="VYQ30_000253"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1965426.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            23213..23818
                     /locus_tag="VYQ30_000254"
     CDS             23213..23818
                     /locus_tag="VYQ30_000254"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1965427.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            23805..25004
                     /locus_tag="VYQ30_000255"
     CDS             23805..25004
                     /locus_tag="VYQ30_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV1965428.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25004..25153
                     /gene="rpmG"
                     /locus_tag="VYQ30_000256"
     CDS             25004..25153
                     /gene="rpmG"
                     /locus_tag="VYQ30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1965429.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         7432 a         4664 c         5695 g         7385 t
ORIGIN      
        1 gcgatagaag aaaaactatc gtctttttct ttgcaaattt ttaagaagag aggtgatctt
       61 gcatggactt tgaatatttt tataacagag aagcggaaag atttaacttc ttaaaagtac
      121 cggagatatt agttgataga gaagaatttc ggggcttatc agcagaagca attatccttt
      181 attccatact tcttaaacag acaggaatgt catttaagaa taactggata gacaaggaag
      241 gcagagtatt tatctatttt actgtcgaag aaattatgaa aagaagaaat atctcaaagc
      301 caactgccat aaaaacatta gatgagcttg atgtaaaaaa aggaatagga ctgatcgaaa
      361 gagtaaggct tggacttggt aagccgaaca tcatttatgt taaagacttt atgagtatat
      421 ttcaggtaaa agaaaatgac ttacagaagt caaaaaactt aacttcagaa gtaaaagatt
      481 ttaacctcag aagtaaagaa aatgaacttc aagaggttaa gaaccttgac tctaactata
      541 tagagaataa taagagtaag tatagtaaga gagaatatag ttttggtgaa aacggacttg
      601 gaacatttca aaatgtgttt ttagctgctg aagatatatc ggatttacaa atcataatga
      661 actcacagct tgagaattac attagacttc ctgcaaaact agaatcctag ttcatgattg
      721 ataataccag caatcaaatt cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt
      781 gttgaaaaca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt gcttctctcc
      841 ttagatagcg catggttaca ggctttatct tcaactgtta gcggtttgag tttgctggat
      901 ttacgtgaag tttgtgcttg aggatatatc ttcatgagcc cttgataacc actgtcagcc
      961 aagattttac cagcttgtcc gatatttctg cgactcattt tgaacaactt catatcatga
     1021 caatagttca cagtgatatc caaagaaaca attctccctt gacttgtgac aatcgcttga
     1081 gtcttcatag cgtgaaattt ctttttacca gaatcattcg ctaattcttt ttttagggcg
     1141 attgattttt acttccgtcg catcaatcat taccgtgtcc tcagagctga gaggagttct
     1201 tgaaatcgta acaccacttt gaacaagagt tacttcaacc cattggctcc gacggagtaa
     1261 gttgctttcg tgaataccaa aatcagccgc aatttcttca taagttcgat attctcgcac
     1321 atattgaaga gtagccataa gaaggtcttc taggcttaat ttaggttttc gtccaccttt
     1381 tgcgtgttta agttgataag ctgtttttaa tacagctaac atctcttcaa aagtcgtgcg
     1441 ctgaacacca acaaaacgct taaatcgtgc atcagttagt tgtttacttg cttcatcatt
     1501 catagaacta ctataccata ttttgtttcg caggaagtct attggaaagt aagaaatatt
     1561 gaagctgagg ctattagaag aaattgtgag cgtggtgcta ttttttcagg taaaataaaa
     1621 tatcacgaag attcacagtt taaaggagat cactatgttg aatgttatgc tgttttagat
     1681 aatacggtta tagcaagaga tagaataaca gtccctatcg atccgttatg tggaaaagat
     1741 tttatagagt agcatataat tgattcttaa ctggaatact cactatctct ttacatcaag
     1801 aaaatgacta aacagggaag tttgccttct tccctttttt tgttatacta gtagaagaaa
     1861 aaaatagaaa gatttgtggg agtgaaaaag tccgggggac tttttcagcc tgagccaaga
     1921 aactcgaaag cttgtaagtc tgattggctt tttcaatgtg aatcttaact tcacactccc
     1981 aaagaggtat tagtgtcgtg tctcaatctt atatcaatgt tatcggtgct ggtttggcag
     2041 gttctgaagc agcttaccaa atcgcagagc gtggtattcc agttaaacta tatgaaatgc
     2101 gtggtgtcaa gtctacaccc cagcataaaa cagacaattt tgctgagttg gtttgttcca
     2161 attctttgcg tggggatgct ttgacaaatg cagttggtct tctcaaggaa gaaatgcgtc
     2221 gcttgggttc tgttatcttg gaatctgctg aggctacacg tgttcctgcc ggtggagccc
     2281 ttgcggtgga tcgtgatggt ttctctcaaa tggtgaccga aaaagtggtc aaccacccct
     2341 tgattgaagt ggtgcgtgat gaaattacag aattgccaac agatgttatt acagttgtgg
     2401 cgactggtcc cttgactagc gatgccctgg ctgaaaagat tcatgccctt aataatggcg
     2461 atggcttcta tttctacgat gcagcagcgc ctattatcga tgtcaacact atcgatatga
     2521 gtaaggtcta tctcaaatct cgttatgata agggagaagc agcctatctc aatgctccaa
     2581 tgaccaagca agagtttatg gatttccatg aagccttggt caatgcggaa gaagcaccgc
     2641 tcaattcttt tgaaaaagaa aagtactttg aaggatgtat gccaatcgaa gttatggcca
     2701 aacgtggcat taaaactatg ctttatggcc ctatgaagcc agtcggtctt gagtatccgg
     2761 acgactacac aggacctcgt gatggagaat ttaaaacacc ttatgcggtt gtgcaacttc
     2821 gtcaggataa tgcagctggt agcctctaca atattgttgg tttccagacc cacctcaaat
     2881 ggggagaaca aaagcgtgtc ttccaaatga ttccgggtct tgaaaatgcg gagtttgtcc
     2941 gttatggtgt catgcatcgc aattcttaca tggattcacc aaatcttctt gagcagactt
     3001 accgttctaa gaaacaacca aatctcttct ttgctggtca gatgacgggt gtggaaggct
     3061 atgttgagtc ggcggcttcg ggcttagttg cgggaattaa cgcagctcgt ctcttcaagg
     3121 aagaaagcga ggttattttc cccgagacga cagcgattgg aagcttagct cattacatta
     3181 cccatgccga cagcaaacat ttccaaccaa tgaatgtcaa ttttgggatc atcaaggagt
     3241 tggaaggcga gcgtatccgt gataagaagg ctcgttatga aaaaattgca gagcgtgccc
     3301 ttgccgactt agaggaattt ttgactgtct aatttttttg aaagaattgt tcatgatact
     3361 ataaaaatct tagaaattgt gataaaatag gtaggatgaa agaaggagag tgaaaatggc
     3421 gaatcccaag tataaacgta ttttaatcaa gttatcaggt gaagcccttg ccggtgaacg
     3481 tggcgtaggg attgatatcc aaacagttca aacaatcgca aaagagattc aagaagttca
     3541 tagcttaggt atcgaaattg cccttgttat tggtggagga aatctctggc gtggagaacc
     3601 tgcagcagaa gcaggtatgg accgtgttca ggcagattac actggaatgc ttgggactgt
     3661 tatgaatgct cttgtgatgg cagattcatt gcaacaagtt ggggttgata cgcgtgtaca
     3721 aacagctatt gctatgcaac aagtggcaga gccttatgtc cgtggacgtg cccttcgtca
     3781 ccttgaaaaa ggccgtatcg ttatctttgg tgctggaatt ggttcaccat acttctcgac
     3841 agatacaaca gcggcccttc gcgcagctga aatcgaagca gatgccatcc tcatggctaa
     3901 aaatggcgtc gatggtgtgt acaatgccga tcctaagaag gacaagacag ccgttaagtt
     3961 tgaagaattg acccaccgtg atgttatcaa caaaggtctt cgtatcatgg actcaacagc
     4021 ctcaaccctc tcaatggaca acgacattga cttggttgtc ttcaacatga accaatcagg
     4081 caacatcaaa cgtgtcgtat ttggtgaaaa tatcggaaca acagtttcaa ataatatcga
     4141 agaaaaggaa taagaaagaa tatggctaac gtaattattg aaaaagctaa agagagaatg
     4201 acccagtctc accaatcact tgctcgtgaa tttggtggta tccgtgctgg tcgtgccaat
     4261 gcaagcttgc ttgaccgtgt acatgtagaa tactatggag tcgaaactcc tcttaaccaa
     4321 atcgcttcaa ttacgattcc agaagcgcgt gttttgttgg taacaccatt tgacaagtct
     4381 tcattgaaag acatcgaacg tgccttgaac gcttctgatc ttggtatcac accggctaat
     4441 gacggttctg tgattcgctt ggttatccca gctcttacag aagaaactcg tcgtgacctt
     4501 gctaaagaag tgaagaaggt cggcgaaaat gctaaagtgg ctgtccgcaa tatccgtcgc
     4561 gatgctatgg acgaagctaa gaaacaagaa aaagcaaaag aaatcactga agacgaattg
     4621 aagactcttg aaaaagatat tcaaaaagta acagacgatg ctgttaaaca catcgacgac
     4681 atgactgcta acaaagagaa agaacttttg gaagtctaaa aataaacaga aaaactcagt
     4741 tggcattgct ggctgagttt tattcgaaag aaggaaatat gaatacaaat cttgcaagtt
     4801 ttatcgttgg actgatcatc gatgaaaatg accgttttta ctttgtgcaa aaggatggtc
     4861 aaacctatgc tcttgctaag gaagagggcc aacatacagt aggggatacg gtcaaaggtt
     4921 ttgcatacac ggatatgaag caaaaactcc gcctgacaac cttagaagtg actgccactc
     4981 aggaccaatt tggttgggga cgtgtcacag aggttcgtaa ggacttgggt gtctttgtgg
     5041 atacaggcct tcctgacaag gaaatcgttg tgtcactcga tattctccct gagctcaagg
     5101 aactctggcc taagaagggc gaccaactct acatccgtct tgaagtggat aagaaagacc
     5161 gtatctgggg cctcttggct tatcaagaag acttccaacg tcttgctcgt cctgcttaca
     5221 acaacatgca gaaccaaaac tggccagcca ttgtttaccg tctcaagctg tcaggaactt
     5281 ttgtttacct accagaaaat aatatgcttg gttttattca tcctagcgag cgttacgcag
     5341 agccacgttt ggggcaagta ttagatgcgc gcgttattgg tttccgtgaa gtggaccgca
     5401 ctctgaacct ctccctcaaa ccacgctcct ttgaaatgtt ggaaaacgat gctcagatga
     5461 ttttgactta tttggaaagc aatggcggtt tcatgacctt aaatgacaag tcatctccag
     5521 acgacatcaa ggcaaccttt ggcatttcta aaggtcagtt caagaaagct ttaggtggtc
     5581 ttatgaaggc tggtaaaatc aagcaggacc agtttggtac agagttgatt tagggaggct
     5641 tatgagaaaa tcattttaca cttggctcat gaccgagcgc aatcctaaaa gtaacagtcc
     5701 caaagcaatt ttggcagacc tcgcttttga agaggcagct tttccaaaac atacagatga
     5761 ttttgatgag gtcagtcgct ttttggagga gcatgccagt ttctctttta acctaggaga
     5821 ttttgacagc atttggcagg aatatctaga acactagcat ttattcattg ggtttgggct
     5881 agtaatttct ccatccctct gctataataa aaagaaataa aaggattaga gaggttcttt
     5941 atttgaagga acattcaata gacattcaac tgagtcatcc agatgacctg tttcatcttt
     6001 ttggttccaa tgagcgccat cttcgtttga tggaagaaga gcttgatgtt gtgattcatg
     6061 ctcgtacgga gattgtccag gttttgggag aagagtctgc ctgtgaggaa gcccgtcaag
     6121 ttattcaggc tttgatggtc ttggtaaatc gtgggatgac cgttggtacg ccagatgtag
     6181 tcactgcgat tagcatggtc aaaaatgatg aaattgacaa gtttgtcgcc ctttacgaag
     6241 aagaaatcat caaagacaat actgggaagc ctatccgagt caaaaccttg ggtcaaaaac
     6301 tttatgtgga cagtgtcaaa cagcatgatg tgacctttgg aatcggacct gcagggacag
     6361 ggaagacctt ccttgcagtg accttggcag tgactgccct taaacgtggg caagtcaaac
     6421 gaattatcct gacacgccca gcggtggaag caggagagag tctaggtttt cttccgggtg
     6481 atcttaagga gaaggtggat ccttaccttc gtcctgttta cgatgccttg tatcaaattc
     6541 ttgggaaaga ccaaacgact cgtctcatgg agcgtgaaat tatcgaaatt gcgccccttg
     6601 cctatatgcg tggccggacc ttggatgatg cctttgtcat tctcgatgag gcgcaaaaca
     6661 cgaccatcat gcagatgaag atgttcttga cgcgtttagg ctttcattct aagatgattg
     6721 tcaatggaga tatcagtcag attgacctgc cacgtaatgt caagtccggt ttgattgatg
     6781 ctcaagagaa actcaagaac atccatcaga ttgactttgt tcatttttca gccaaggatg
     6841 tggttcgcca tccagttgtc gctcagatta tccgagccta tgaaccagct ccagttaaaa
     6901 aagaagagag tgaagaatta gagtctcaac ctctgtctga aggatagttc ttttgtactt
     6961 aaagttatct caatgctaag tataaatttg ctttttaaat gttttattca atatccttat
     7021 attagattag aaacgtttca agcggaggaa acgtatggaa tcaatttttg taaaacttgc
     7081 ccagtatccg tctatagaaa cggagcgttt attgcttaga cctgtaactt tggatgatgc
     7141 ggaagcaatg tttgactatg cctcggacaa gggtaataca cgttacactt ttccaaccaa
     7201 tcaaagcttg gaagaaacca agaataacat tgctcagttc tatttggtta atcccttggg
     7261 acgttgggga atagaactaa aaagcaatgg tcagtttatt ggaaccattg acttgcacaa
     7321 gattgattct gttcttaaga aggcagctat tggctacatt atcaataaaa agtattggaa
     7381 tcaaggatta acgacagaaa ccaatcgtgc tgtgattgag ctagcttttg agaagatagg
     7441 aatgaataag ttagttgctt tatacgataa ggctaatccc gcatcaggaa aggtcatgga
     7501 gaaatcagga atgcgttttt cccatgcaga accatatgct tgtatggacc agcatgaaaa
     7561 aggccgaatc gtgacaagag ttcattatgt cttgaccaag gaagactatt ttgcaaataa
     7621 ataagcagtt gaaaagaaat ttttcgactg ttttttcttc ctcttacgaa taatctaaga
     7681 gaggagaaaa tatggaagca attatcgaga aaatcaaaga gtataaaatc atcgtcatct
     7741 gtactggtct gggcttgctt gtaggaggat ttttcctgct aaaaccagct ccacaaacac
     7801 ctgtcaaaga gacgaatttg caggctgaag tcgcagctgt ttccaaggat ttggtatccg
     7861 aaaaggaagt gaacaaggaa gaaaaggaag aaccccttga acaagatcta atcacagtag
     7921 atgtcaaagg tgctgtcaaa tcgccaggga tttatgactt gcctgtaggt agtcgagtca
     7981 atgatgctgt tcagaaggct ggtggcttga cagagcaagc agacagcaag tcgctcaatc
     8041 tagctcagaa agttagtgat gaggctctgg tttacgttcc tactaaggga gaagaagcag
     8101 ttagccaaca gactggtttg gggacagctt cttcaataag caaggaaaag aaggtcaatc
     8161 tcaacaaggc cagtctggaa gaactcaagc aggtcaaggg actgggagga aaacgagctc
     8221 aggacattat cgaccatcgt gaggcaaatg gcaagttcaa gtcagtagac gagctcaagc
     8281 aggtctctgg cattggtgcc aagactatag aaaagcttaa agactatgtt acagtggatt
     8341 aagaatttct ctattcccct aatttacctg agttttctat tactttggct ttattacgct
     8401 attttctcag catcctatct tgctttgttg ggctttgttt ttctgctagt ctgtctcttt
     8461 atccaatttc cgtggaaatc tgctggtaaa gttctaataa tttgcggaat ctttggattt
     8521 tggtttgttt ttcaaaattg gcaacagagt caagcgagtc aaaatctggc ggattctgtt
     8581 gaaagggtac ggattctgcc tgacactgtt aaggtcaatg gtgatagtct gtcctttcgc
     8641 ggcaaggctg atggacgcat ttttcaagtc tattataaac tccagtccga ggaggagaaa
     8701 gaagcctttc aagctttaac cgacctgcat gagataggac tagaagggaa gctttcggag
     8761 ccagaagggc agagaaattt tggtggcttt aattaccaag cctatctgaa gactcaggaa
     8821 atttaccaga ctctcaatat caaaaaaatc cagtcacttc aaaagattgg cagttgggat
     8881 ataggagaaa acttgtccag tttacgtcga aaggctgtgg tttggattaa gacgcacttt
     8941 ccagacccta tgcgcaatta catgacagga cttttgctgg gacatctgga caccgacttt
     9001 gaggagatga atgagcttta ttccagtcta ggaattatcc acctctttgc cctatctggc
     9061 atgcaggtag gttttttcat gaatggattt aagaaacttc tcttgcgatt gggcttgacc
     9121 caagaaaagt tgaaatggct aacttatccc ttttccctta tctatgcggg actaactgga
     9181 ttttcagcat cggttattcg cagtctcttg caaaagctac tggctcaaca tggggttaag
     9241 ggcttggata attttgcctt gacggtgctt gtcctcttta ttgtcatgcc aaactttttc
     9301 ttgacagcag gaggagtctt gtcctgcgct tatgctttta tcctgaccat gaccagcaaa
     9361 gaaggggagg ggctcaaggc tgttgctagt gaaagtctag tcatctcctt gggcatattg
     9421 cccattctat ccttctattt tgcggaattt caaccttggt ctatcctttt gacctttgtc
     9481 ttttcctttc tatttgactt gaccttctta ccgctcttgt ctattttatt tgtcctttcc
     9541 tttctctatc cagtcattca gctgaacttt atctttgaat ggttagaggg cattattcgc
     9601 ttggtctcgc aggtggcaag gagaccgctt gtctttggtc aacccaacgc atggttttta
     9661 atcctattgt taatttcctt ggctttggtc tatgatttga gaaaaaacat taaaaagcta
     9721 acggtattgt gcttattgat tacagggctc tttctcctga ccaagcatcc actggaaaat
     9781 gaaatcacca tgctggatgt ggggcaagga gaaagtattt tcctatggga tataactggg
     9841 aaaaccattc tcatagatgt cggtggcaag gcagaatctt ataagaaaat cgaaaaatgg
     9901 caagaaaaga tgacgaccag caatgcccag cgaaccttga ttccctatct caaaagtcga
     9961 ggagtagcta agattgacca gctaattttg actaacacgg acaaggagca tgttggagat
    10021 ttgtcagagg tgaccaaggc tttccatgta ggggagattc tagtatcaaa agacagtctg
    10081 aaacagaagg aatttgtggc agaactacag gcgactcaaa caaaggtgcg tagtatgaca
    10141 gtaggggaga acttgcccat ttttggaagt cagttagaag ttctatctcc aaggaaaatg
    10201 ggagatggag gacacgatga taccctagtt ctgtatggga aattcttgga taagcaattt
    10261 ctcttcacgg gaaatttgga ggagaaagga gagaaggact tgctgaagca atatccttac
    10321 ttagaggtgg atgtattgaa agctgcccaa catggctcta aaaagtcatc aagtccagcc
    10381 tttctagaaa aactcaaacc agagcttact cttatctcag ttggaaagag caatcgaatg
    10441 aaactccccc atcaggaaac attgacacga ctggaaggta tcaatagcaa agtttaccga
    10501 actgaccagc aaggagctat acgttttaag gggttggata gttggaaaat cgaaagtgtt
    10561 cgataggaag gataaatgtt gtagattagt gaaataaact aaaaatttgt tgcataataa
    10621 tgataaaaat ggtataatga aaacgtattc aatattgagg atataaaatc attaaaaatc
    10681 agcaaaagtt gttttattag ttagtttata atctattggt cttcttcagt ccagtgtatc
    10741 tgctgtgaca gccactaaaa gttacaagta tgattggaat acggttttgg aatatagtac
    10801 caactatcac gaccatcagt atgcttggat tccggaatgg tctcgttatt acagctattc
    10861 tgagtataaa gttggcggag gctggaacta cgctcgttat gaggtcataa actactatac
    10921 tggaggttat taatccttaa agagtgagaa aaggagggct ggatatgtta aatcttactc
    10981 atgttacctt aaaaacgcga caagtcatct tgcaagatgc ggattttacc tttaaaaagg
    11041 gtaggattta tggccttctt gctatcaatg gctcgggaaa gacgacacta ttccgagcta
    11101 tgagcaagtt gcttcccctt agtagtggac acatcgcagt tcctccttct ttgttttatt
    11161 atgagagcgt tgaatggctg gatggaaact taagtgggat ggactacctt cgtctcataa
    11221 aaaacatctg gaagtcagac ctaaacttga gagatgaaat cgcctactgg gaaatggctg
    11281 actatatcag tcttcccatc cgcaagtatt ccttaggcat gaagcaacgc ttggtgattg
    11341 ctatgtattt tctcagtcag gccaaatgct ggctcatgga tgagattaca aatggcttag
    11401 acgagtatta tcgacagaag ttttttgata ggctagcaca aatcgataga caagaacagc
    11461 tggttctttt aagttcccac tataaggaag agttggttga tatctgcgat agagtagtaa
    11521 ccattcatca ggggcagata gaagaggttt agtttatgaa agatgttagt ctatttttat
    11581 tgaaaaaagt tttcaaaagt cgtttaaact ggattatctt acttttattt gcatctgtac
    11641 tcggtgttac cttttattta aatagtcaga ctgcaaactc acacagcttg gagagcaggt
    11701 tggaaagtcg cattgcagcc aacgagaggg ctatcaatga aaatgaagag aaactctccc
    11761 aaatgtctga taccagctcg gaggaatacc agtttgctaa aaataattta gacgtgcaaa
    11821 aaaatctttt gacgcgaaag acagaaattc taactttact aaaagaaggg cgctggaaag
    11881 aagcctacta tctgcagtgg caagatgaag agaagaatta tgaatttgta tcaaatgacc
    11941 cgactgctag ctctggctta aaaatggggg ttgaccgcga acggaagatt taccaagccc
    12001 tgtatccctt gaacataaaa gcacatactt tggagtttcc gacccacggg attgatcaga
    12061 ttgtctggat tttagaggtt atcatcccaa gcttgtttgt ggttgcgatt atttttatgc
    12121 taacacaact atttgcagaa agatatcaaa atcatctgga cacagctcac ttatatcctg
    12181 tttcaaaagt gacatttgca atatcctctc ttggagttgg agtgggatat gtaactgtgc
    12241 tgtttatcgg aatctgtggc ttttcttttc tagtgggaag tctgataagt ggttttggac
    12301 agttagatta tccctaccca atttatagct tagtgaatca agaagtaact attgggaaaa
    12361 tacaagatgt attatttcct ggcttgctct tagctttctt agcctttatc gtcattgtgg
    12421 aagttgtgta cttgattgct tactttttca agcaaaaaat gcctgtcctc tttctttcac
    12481 tcattgggat tgttggctta ttgtttggca tccaaacaat tcagcctctt caaaggattg
    12541 cacatctgat tccctttact tacttgcgtt cagtggagat tttatctgga agattatcta
    12601 agcagattga taatgtcgat ctaaattgga gcatggggat ggtcttactt ccttgcctga
    12661 ttatcctttt gttagtgggg attctattta tcgaaagatg gggaagttca cgtaaaaagg
    12721 aagtttttaa agtttagttt tcctataggg aagctaaaga aaaactaaca tagggagaga
    12781 atcaacttgg ttctctcttt ttgattcgaa aaccaaacca aaatacaaac acaaactttt
    12841 caaaaaataa ctttttatct tgacaagagc tagaaaactt ggtatcatat aaaagttgag
    12901 aaaagcagaa gtgagagctt ctcgccttgt gacattaagt tgcctggccc tacggatgaa
    12961 aagtttcgaa gaaacgctat cataacgtgc gggcttgtat atttacgagt ccgctattgt
    13021 ttttctctaa taaaacaaaa gaggtgaaaa ccatagcaaa gcaagactta ttcatcaatg
    13081 atgagattcg tgtacgtgaa gttcgcttga ttggtcttga gggagaacag ctaggcatca
    13141 agccactcag tgaagcgcaa gctttggctg ataacgctaa tgttgaccta gtattgattc
    13201 aaccccaagc caaaccgcct gttgcaaaaa ttatggacta cggtaagttc aaatttgagt
    13261 accagaagaa gcaaaaagaa caacgtaaaa aacaaagcgt tgttactgtg aaagaagttc
    13321 gtctaagtcc aacaattgac aagggtgact ttgatacaaa acttcgtaat gcacgtaagt
    13381 tccttgaaaa aggaaataaa gttaaggtat ctattcgctt taagggtcgt atgattaccc
    13441 ataaagagat tggtgcaaaa gttttagccg agtttgctga agcaactcaa gatattgcca
    13501 tcatcgaaca acgtgctaaa atggatggac gtcaaatgtt catgcaattg gcgccagcga
    13561 ctgacaaaaa ataattgtca gaaagttaaa taaaggagaa aaaatcatgc caaaacaaaa
    13621 aacacaccgc gcatcagcta aacgtttcaa acgtacaggt tctggtggac ttaaacgttt
    13681 ccgtgcttac acttctcacc gtttccacgg aaaaactaag aaacaacgtc gtcatcttcg
    13741 taaagcatct atggtgcatt caggagatta caaacgtatc aaagcaatgc ttactcgctt
    13801 gaaataatag cttgactgta agtaaacaat tctagaaaat atttggagga aataaaacat
    13861 ggcacgtgtt aaaggtggcg ttgtatcacg caaacgtcgt aaacgtattc ttaaattagc
    13921 aaaaggttac tatggagcta aacacatctt gttccgtact gcaaaagaac aagtaatgaa
    13981 ctcttactac tatgcatacc gtgaccgtcg tcagaaaaaa cgtgacttcc gcaaattgtg
    14041 gattactcgt atcaatgcgg cggctcgtat gaacggactt tcatactcac aattgatgca
    14101 tggtttgaaa ttggctgaga tcgaagttaa ccgtaaaatg cttgctgact tggctgttaa
    14161 cgatgcagta gctttcacag ctcttgcaga tgcagctaaa gcaaaacttg gtaaataaca
    14221 actgatgaga ctggaacagg attgtccagt ctttttaaaa ataaaggaga aagatatggc
    14281 ttcaaaaatg ctacacactt gcttacgagt agaaaatctt gaaaaatcaa tcgcatttta
    14341 tcaagatgct tttggtttta aagaattgcg tcgcagagat tttccagacc atgccttcac
    14401 gattgtctat ctaggtcttg agggtgatga ttatgagttg gagttgactt ataactacga
    14461 tcacggtccg tatgtggttg gtgatgggtt tgcccatatc gccctcagta cacctgatct
    14521 tgaggcactt catcaagaac acagtgcaaa aggctatgaa gtgactgagc caaatggtct
    14581 accaggaact acaccaaact attactttgt taaagaccct gatggatata aggtcgaagt
    14641 cattcgtgaa aaataatttc gtgcaagtta agtagaatat tcgttttcta cttgactttt
    14701 tttagctgaa agagtatact aataaaaatt caacctttaa ggggagtcca gagagactca
    14761 caaggtgtca gataaaagaa tagtgcaatt ttctagagga gaccttttga gtgtgctctc
    14821 ttgtgttgta cgattttaac tgaggccttg cactagcaag gtcttttctt tatctggtcc
    14881 ccttaaaatt taaggaggaa aagttatgaa tctcacatgt aagaagcgtt tgggtgtcat
    14941 tcggttggaa accatgaagg tggttgcaca agaggaaatc gcgccagcaa tctttgaatt
    15001 agtcctagaa ggagaaatgg ttgaagccat gcgagcaggc caatttcttc atctgcgtgt
    15061 accagacgat gcccatctct tacgtcgtcc tatttcaatt tcgtctattg acaaggcaaa
    15121 caagcagtgt cacctcattt atcggattga gggagctggg actgcaattt tttcaacctt
    15181 aagtcaggga gacactcttg atgtgatggg gcctcaggga aatggttttg acttgtctga
    15241 ccttgatgag cagaatcagg ttctccttgt tggtggtggg attggtgttc cacccttgct
    15301 tgaggtggcc aaggaattgc atgaacgtgg agtgaaagta gtgacagtcc tcggttttgc
    15361 taataaggat gctgttattt tgaaaacgga attggctcag tatggtcagg tctttgtaac
    15421 gacagatgat ggttcttatg gcatcaaggg aaatgtttcc gttgttatca atgatttaga
    15481 tagtcagttt gatgctgttt actcgtgtgg ggctccagga atgatgaagt atatcaatca
    15541 aacctttgat gatcacccaa gagcctattt atctctggaa tctcgtatgg cttgtgggat
    15601 gggagcttgc tatgcctgtg ttctaaaagt accagaaagc gagacggtca gccaacgcgt
    15661 ctgtgaagat ggtcctgttt tccgcacagg aacagttgta ttataaggag aaaattatga
    15721 ctacaaatcg attacaagtg tctctacctg gtttggattt gaaaaatccg attattccag
    15781 catcaggctg ttttggcttt ggacaagagt atgccaagta ctatgattta gaccttttag
    15841 gttctattat gatcaaggcg acaacccttg aaccacgttt tgggaatcca actccaagag
    15901 tggcagagac gcctgctggt atgctcaatg caattggctt gcaaaatcct ggtttagagg
    15961 ttgttttggc tgaaaagcta ccttggctgg aaagagaata tccaaatctt cctattattg
    16021 ccaatgtagc tggtttttca aaacaagagt atgcagctgt ttctcatggg atttccaagg
    16081 caactaatgt aaaagctatc gagctcaata tttcttgtcc caatgttgac cactgtaatc
    16141 atggactttt gattggtcaa gatccagatt tggcttatga tgtggtgaaa gcagctgtgg
    16201 aagcctcaga agtgccagtt tatgtcaaat taaccccgag tgtgaccgat atcgttactg
    16261 tcgcaaaagc tgcagaagat gcgggagcaa gtggcttgac catgatcaat actctggttg
    16321 gaatgcgctt tgacctcaaa accagaaaac caatcttggc caatggaaca ggtggaatgt
    16381 caggtccagc agtctttcca gtagccctca aactcatccg ccaagtagcc caaacaacag
    16441 acctgcctat cattggaatg gggggagtgg attcggctga agctgcccta gaaatgtatc
    16501 tggctggggc atctgctatc ggagttggaa cagctaactt taccaatcct tatgcctgcc
    16561 ctgacatcat cgaaaattta ccaaaagtca tggataaata cggtattagc agtctggaag
    16621 aactccgtca ggaagtaaaa gagtctctga ggtaaactgc aatcaatctg ttcttgattt
    16681 tttattagtt tgtaatatga atttaggaga attttggtac aataaaataa ataagaacag
    16741 aggaagaagg ttaatgaaga aagtaagatt tattttttta gctctgctat ttttcttagc
    16801 tagtccagag ggtgcaatgg ctagtgatgg tacttggcaa ggaaaacagt atctgaaaga
    16861 agatggcagt caagcagcga atgagtgggt ttttgatact cattatcaat cttggttcta
    16921 tataaaagca gatgctaact atgctgaaaa tgaatggcta aagcaaggtg acgactattt
    16981 ttacctcaaa tctggtggct atatggccaa atcagaatgg gtagaagaca agggagcctt
    17041 ttattatctt gaccaagatg gaaagatgaa aagaaatgct tgggtaggaa cttcctatgt
    17101 tggtgcaaca ggtgccaaag taatagaaga ctgggtctat gattctcaat acgatgcttg
    17161 gttttatatc aaagcagatg gacagcacgc agagaaagaa tggctccaaa ttaaagggaa
    17221 ggattattat ttcaaatccg gtggttatct actgacaagt cagtggatta atcaagctta
    17281 tgtgaatgct agtggtgtca aagtacagca aggttggctt tttgacaaac aataccaatc
    17341 ttggttttac atcaaagaaa atggaaacta tgctgataaa gaatggattt tcgagaatgg
    17401 tcactattat tatctaaaat ccggtggcta catgacagcc aatgaatgga tttgggataa
    17461 ggaatcttgg ttttatctta aatctgatgg gaaaatagct gaaaaagaat gggtctacga
    17521 ttctcatagt caagcttggt actacttcaa atctggtggt tacatgacag ccaatgaatg
    17581 gatttgggat aaggaatctt ggttttacct caaatctgat gggaaaatag ctgaaaaaga
    17641 atgggtctac gattctcata gtcaagcttg gtactacttc aaatccggtg gttacatgac
    17701 agccaatgaa tggatgtggg ataaggaatc ttggttttac ctcaagtctg atggcaaaat
    17761 ggctgaaaaa gaatgggtct atgattctca tagtcaagcc tggtactact tcaaatctgg
    17821 tggctacatg gcgaaaaatg agacagtaga tggttatcag cttggaagcg atggtaaatg
    17881 gcttggagga aaagctacaa atgaaaatgc tgcttactat caagtagtgc ctgttacagc
    17941 caatgtttat gattcagatg gtgaaaagct ttcctatata tcgcaaggta gtgtcgtatg
    18001 gctagataag gatagaaaaa gtgatgacaa gcgcttggct attactattt ctggtttgtc
    18061 aggctatatg aaaacagaag atttacaagc gctagatgct agtaaggact ttatccctta
    18121 ttatgagagt gatggccacc gtttttatca ctatgtggct cagaatgcta gtatcccagt
    18181 agcttctcat ctttctgata tggaagtagg caagaaatat tattcggcag atggcctgca
    18241 ttttgatggt tttaagcttg agaatccctt ccttttcaaa gatttaacag aggctacaaa
    18301 ctacagtgct gaagaattgg ataaggtatt tagtttgcta aacattaaca atagcctttt
    18361 ggagaacaag ggcgctactt ttaaggaagc cgaagaacat taccatatca atgctcttta
    18421 tctccttgcc catagtgccc tagaaagtaa ctggggaaga agtaaaattg ccaaagataa
    18481 gaataatttc tttggcatta cagcctatga tacgacccct tacctttctg ctaagacatt
    18541 tgatgatgtg gataagggaa ttttaggtgc aaccaagtgg attaaggaaa attatatcga
    18601 taggggaaga actttccttg gaaacaaggc ttctggtatg aatgtggaat atgcttcaga
    18661 cccttattgg ggcgaaaaaa ttgctagtgt gatgatgaaa atcaatgaga agctaggtgg
    18721 caaagattag tactataagt gaatatgatt tgagtgaata gtaagttaaa aatcctgatt
    18781 tcaagtaaaa tcaggatttt ttcatggatg caattttttt ggagtctggt gtgacgcgga
    18841 gggtcttttg tcctgtgtaa gtgacaaagc cgggttttcc accagttggt ttattgagtt
    18901 ttttgacttc aatcatatct acctgcacca gattcgacag gcgcccttga gagaagtagg
    18961 cagctaactc tgctgcgtct gtcttgactg catcagatgg gtcaagattt cctgagatga
    19021 caacatggct tccaggaatg tccttagcat ggaaccaaag ttcctccttg cgggccattt
    19081 taaaggtcaa ttcctcattt tgaagattgt ttcgtccgac atagatgatg gttttgccat
    19141 cgcttgctag atattgttct agttttttgc gtttctggat tttctcccgt tgtcttctgc
    19201 ggataaaacc tgtttgaatc aattcttcac ggatttcagc gatttcttcc agtccagctt
    19261 ggttgaggac ggtttctaca ctttccagat agagaatagt ggctttggtt tcttcaatca
    19321 aatcagtcaa gtatttgaca gcttctttga gtttctgata ccgtttaaaa tagcgttggg
    19381 cattctggtt gggagtcaga gccttatcaa gcgcaatcat gataggttgg ttggtatagt
    19441 agttgtctag gataacctgg tcttggtcgt taggcacttg gtggaggaag gttgtcagca
    19501 attctccttt ttgacgaaat tcttcagcgt tgtctgtcgc cagtaactct ttttcctgtt
    19561 ttttgagttt gtgtcggttt ttctgaagtt cattttcaac acgacgaatc agttcactgg
    19621 cctgctgttt gacgcggtcg cgctcagcct tatccttata gtaggtgtcc aacaaatcag
    19681 aaagatttgc aaaaggctct cccacctgat ttgcaaaagg aactggactg aaggaagtct
    19741 cagtcaagca tggcttggtt tcttgattga aaaaatttcg gaaagcggaa agtttttcac
    19801 taaccagtat cctttccaat tcatttgccg tatcgcgtcc cagaccttga aagaggcttt
    19861 gaagattttt tgctgttagt tcttgggttt gcaggatttc aaagagcttt tcatccttga
    19921 tagtaaaagg attgagagat tttgtacttg gcggagcgat ataggtcgat cctggaagta
    19981 aggtgcggta gctattttgt gaaaagccga cgtgtttgat aacttcgagg attttatgac
    20041 tgcttttatc gaccagtaga atattactgt gtttccccat aatttcgata atcaaggtag
    20101 cctggatatg gtctccaatc tcgtttttat tggaaactgt aatttccaca atacggtcat
    20161 tttccacttg ctcaatcgac tcaatcaggg ccccctgcaa atactttctc aaaaccatga
    20221 taaaggtaga aggttgggct ggattttcaa aagtcgtttg ggtcagctga atgcgtccaa
    20281 aaactggatg ggcagaaagg agcaggcgat ggctttggcg attgctgcgg atttgcaaga
    20341 ccaactcttg ttcaaaaggc tgattgattt tctggatgcg accattcact aattcgcttc
    20401 gcaattcctc aactatgtgg tgtaaaaaaa atccgtcaaa tgacatcgtt ctctccttgt
    20461 gattgtattc catagtatta tatcaaaaag gtagaataaa atcatggaaa tgtggtataa
    20521 taaagccaag taaagagaaa cgagaagcac atgtatattg aaatggtaga tgaaactggt
    20581 caagtttcaa aagaaatgtt gcaacaaacc caagaaattt tggaatttgc agcccaaaaa
    20641 ttaggaaaag aagacaagga gatggcagtc acttttgtga ccaatgagcg tagtcatgaa
    20701 cttaatctgg agtaccgtga caccgaccgt ccgacagatg tcatcagcct tgagtataaa
    20761 ccagaattgg aaattgcctt tgacgaagag gatttgcttg aaaatccaga attggcagag
    20821 atgatgtctg agtttgatgc ctatattggg gaattgttca tctctatcga taaggctcat
    20881 gagcaggccg aagaatatgg tcacagcttt gagcgtgaga tgggcttctt ggcagtacac
    20941 ggctttttac atattaacgg ctatgatcac tacactccgg aagaagaagc ggagatgttc
    21001 ggtttacaag aagaaatttt gacagcctat ggactcacaa gacaataaac gaaaatggaa
    21061 aaatcgtgac ttgatatcca gtttagaatt tgctttgaca ggtattttta ctgctatcaa
    21121 ggaagaacgc aatatgcgaa aacacgcagt gacggctcta gtggtcatcc ttgcaggttt
    21181 tgtttttcag gtgtcacgaa tcgaatggct ctttctccta ttgagtattt tcttggtagt
    21241 agcctttgag attatcaact ctgctattga aaatgtggtg gatttggcca gtcactatca
    21301 cttttccatg ctggctaaaa atgccaagga tatggcggcc ggcgcggtat tagtggtttc
    21361 tcttttcgca gccttaacag gcgcattgat ttttctccca cgaatctggg atttattatt
    21421 ttaaacagta agaggaaatt atgactttta aatcaggctt tgtagccatt ttaggacgtc
    21481 ccaatgttgg gaagtcaacc tttttaaatc acgttatggg gcaaaagatt gccatcatga
    21541 gtgacaaggc gcagacaacg cgcaataaaa tcatgggaat ttacacgact gataaggagc
    21601 aaattgtctt tatcgacaca ccagggattc acaagcctaa aacagctctc ggagatttca
    21661 tggttgagtc tgcctacagt acccttcgcg aagtggacac tgttcttttc atggtgcctg
    21721 ctgatgaagc gcgtggtaag ggggacgata tgattatcga gcgtctcaag gctgccaagg
    21781 ttcctgtgat tttggtggtg aataaaatcg ataaggttca tccagaccag ctcttgtctc
    21841 agattgatga cttccgtaat caaatggact ttaaggaaat tgttccaatc tcagcccttc
    21901 agggaaataa cgtgtctcgt ctagtggata ttttgagtga aaatctggat gaaggtttcc
    21961 aatatttccc gtctgatcaa atcacagacc atccagaacg tttcttagtt tcagaaatgg
    22021 ttcgcgagaa agtcttgcac ctaactcgtg aagagattcc gcattctgta gcagtagttg
    22081 ttgactctat gaaacgagac gaagagacag acaaggttca catccgtgca accatcatgg
    22141 tcgagcgcga tagccaaaaa gggattatca tcggtaaagg tggcgctatg cttaagaaaa
    22201 tcggtagcat ggcccgtcgt gatatcgaac tcatgctagg agacaaggtc ttcctagaaa
    22261 cctgggtcaa ggtcaagaaa aactggcgcg ataaaaagct agatttggct gactttggct
    22321 ataatgaaag agaatactaa gtagaggtag gctcatgcct gcttcttgtt tttacagaag
    22381 gaggacttat gcctgaatta cctgaggttg aaaccgtttg tcgtggctta gaaaaattga
    22441 ttataggaaa gaagatttcg agtatagaaa ttcgctaccc caagatgatt aagacggatt
    22501 tggaagagtt tcaaagggaa ttgcctagtc agattatcga gtcaatggga cgtcgtggaa
    22561 aatatttgct tttctgcctg acagacaagg tcttgatttc ccatttgcgg atggagggca
    22621 agtattttta ttatccagac caagtgcctg aacgcaagca tgcccatgtt ttcttccggt
    22681 ttgaagatgg gggcacgctt gtttatgagg atgtacgcaa gtttggaacc atggaactct
    22741 tggtgcctga ccttttagac gcctacttta tttctaaaaa attaggtcct gaaccaagcg
    22801 aacaagactt tgatttacag gtctttcaag ctgcccttgc caagtccaaa aagcctatca
    22861 aatcccatct cctagaccag accttggtag ctggacttgg caatatctat gtggatgagg
    22921 ttctctggcg agctcaggtt catccagcta gaccttccca gactttgaca gcagaagaag
    22981 cgactgccat tcatgaccag accattgctg ttttgggcca ggctgttgaa aaaggtggct
    23041 ccaccattcg gacttatacc aatgcctttg gggaagatgg aagcatgcag gattttcatc
    23101 aggtctatga taagactggt caagaatgtg tacgctgtgg taccatcatt gagaaaattc
    23161 aactaggcgg acgtggaacc cacttttgtc caaactgtca aaggagggac tgatgggaaa
    23221 aatcatcgga atcactgggg gaattgcctc aggtaagtca actgtgacaa attttctaag
    23281 acagcaaggc tttcaagcag tggatgccga cgcagtcgtc caccaactac agaaacctgg
    23341 tggtcgtctg tttgaggctc tagtacagca ctttgggcaa gaaatcattc ttgaaaacgg
    23401 agaactcaat cgccctctcc tagctagtct catcttttca aatcctgaag agcaaaaatg
    23461 gtctaatcaa attcaagggg agattatccg tgaggaactg gctactttga gagaacagtt
    23521 ggctcagaca gaagagattt tcttcatgga tattccccta ctttttgagc aggactacag
    23581 cgattggttt gctgagactt ggttggtcta tgtggaccga gatgcccaag tagaacgctt
    23641 aatgaaaagg gaccagttgt ccaaagatga agctgagtct cgtctggcag cccagtggcc
    23701 tttagaaaaa aagaaagatt tggccagcca ggttcttgat aataatggca atcagaacca
    23761 gcttcttaat caagtgcata tccttcttga gggaggtagg caagatgaca gagattaact
    23821 ggaaggataa tctgcgtatt gcctggtttg gtaattttct gacaggagcc agtatttctt
    23881 tggttgtacc ttttatgccc atcttcgtgg aaaatctagg tgtagggagt cagcaagtcg
    23941 ctttttatgc aggcttagca atttctgtct ctgctatttc cgcggcgctc ttttctccta
    24001 tttggggtat tcttgctgac aaatacggcc gaaaacccat gatgattcgg gcaggtcttg
    24061 ctatgactat cactatggga ggcttggcct ttgtcccaaa tatctattgg ttaatctttc
    24121 ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa tgcaacggca ctgatagcca
    24181 gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac tttgtctaca ggcgtagttg
    24241 caggtactct aactggtccc tttattggtg gctttattgc agaattattt ggcattcgta
    24301 cagttttctt actggttggt agttttctat ttttagctgc tattttgact atttgcttta
    24361 tcaaggaaga ttttcaacca gtagccaagg aaaaggctat tccaacaaag gaattattta
    24421 cctcggttaa atatccctat cttttgctca atctcttttt aaccagtttt gtcatccaat
    24481 tttcagctca atcgatcggc cctattttgg ctctttatgt acgcgactta gggcagacag
    24541 agaatcttct ttttgtctct ggtttgattg tgtccagtat gggcttttcc agtatgatga
    24601 gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa tcatcgtctc ttggttgtcg
    24661 cccagtttta ttcagtcatc atctatctcc tctgtgccaa tgcctctagc ccccttcaac
    24721 taggactcta tcgtttcctc tttggcttgg gaaccggtgc cttgattccc ggggttaatg
    24781 ccctactcag caaaatgact cccaaagccg gcatttcgag ggtctttgcc ttcaatcagg
    24841 tattctttta tctgggaggt gttgttggtc ccatggcagg ttctgcagta gcaggtcaat
    24901 ttggctacca tgctgtcttt tatgcgacaa gcctttgtgt tgcctttagt tgtctcttta
    24961 acctgattca atttcgaaca ttattaaaag taaaggaaat ctagtgcgag taaaaattaa
    25021 tctcaaatgc tcctcttgtg acagtatcaa ttacctaacc agtaaaaatt caaaaaccca
    25081 tccagacaag attgaggttt taaagtattg tcccaaggaa agaaaagtaa ccctacatct
    25141 tgaatctaag tagattttat ggtaaaataa taggga
//
LOCUS       DAVMEF010000009        24714 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMEF010000009 DAVMEF010000000
VERSION     DAVMEF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.009"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            231..968
                     /locus_tag="VYQ30_000257"
     CDS             231..968
                     /locus_tag="VYQ30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1965430.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            965..2656
                     /locus_tag="VYQ30_000258"
     CDS             965..2656
                     /locus_tag="VYQ30_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1965431.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            2772..3335
                     /locus_tag="VYQ30_000259"
     CDS             2772..3335
                     /locus_tag="VYQ30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1965432.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            3357..9299
                     /locus_tag="VYQ30_000260"
     CDS             3357..9299
                     /locus_tag="VYQ30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1965433.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEDTQPEVVETKDEAANQP
                     VEEPKVETPAVEKQTEPTEEPKVEQVGEPVAPREDEKAPVSPEKQPEAPEEEKTAEET
                     PKQEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASE
                     PVKQPEVNSETKAEKVAANTDAKQSEVNSETASLKTAISGLNTDKVELENQLKIAQGK
                     TETDFSMESWTVLSTAKNKAQEVKDNGTATQEQINEAEKSLKTALADLSVDKTALGSA
                     IDTATKKNKENYTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELS
                     EKPRLTLSIEKRDIDRKVTVTYTLENPANTQIKSITATLKKGEEVVKDFVLTEENLKT
                     NHLTALFEKLDYYKEYTLSTDMVYNRGNDDETESISEELIQLNLKKLELKDIQTVSLM
                     KFENGQESQVTHLSDKPTDLSKLYLKVTSSTSKDAVLAVSSIEEEIVENKKIFKIHAD
                     TPELVVRKKDGSLSKGFDYYMERVIPHDGDIYYDFKDLISAMTSNPTGTFILGRDISS
                     RNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIFDLEYPLFDTIKSGVVKDIDFKHVNM
                     VFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEGRDHVAGLVNNLEGNSEIENISFT
                     GKIKSKGGNSITAGIAGRNILSRVKRAYVNANIEVLGSTNSSMLVAVNGTTLNASGGW
                     GAWGRLTESVAKGTLEIKRSGQAGGVTATVWPYGAIDKVVSYAKVTKGKELFGSDGDL
                     NNNWFMQKINNIFGVQGISSGDSGNDSKFKRISEEEAKQKVASYNITAPNLMSDSSLL
                     VDRLNESWKNTDQFESIQDYQSQNQLIYQNLTKFTPYYNKEFIVHEGNALTPEQEILK
                     TKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQKRYKVTSTGKFSITNLPEYQVEDL
                     NVVYTSEHIVHPLDSSLINNLVEELKKVELYTESTYQVLGIDKDNANKLNRTKRLFLD
                     ESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAALRQKIMDNKTAILLALTYINRYY
                     DVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGSSGENRLKGSENAETFKQLFASET
                     KQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQFEERPSLVEEIKDAKYRVYDNLT
                     APYYQGYILPLLTLKNTHLAILSNYSTMTFVSREKRPNWKNEDFDKWVKYVATAHRNH
                     VDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEWVDQNAVDRKGRDYAPAREFFNLV
                     GGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYGISVFTHELTHVNDTWIYLGGYGR
                     RENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFERKNDGDLIYNASPTQFENRKELDS
                     YMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKVEPKVVSQTAQYDTVRQLTAEEKE
                     KLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSYIAIDYMTGIYGGGKNSVGSPGAL
                     MFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEGHAGLSDNFIISKISKGEFLTMEA
                     FKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQEGFKQAVERDLKKNQLDERETRN
                     FKFQVFRQLLQQTDSFKTSIFR"
     gene            9360..11081
                     /gene="pabB"
                     /locus_tag="VYQ30_000261"
     CDS             9360..11081
                     /gene="pabB"
                     /locus_tag="VYQ30_000261"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1965434.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLTVHKVPLLGEYLLYFTVHDRVETSPVPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLEASIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            11082..11699
                     /locus_tag="VYQ30_000262"
     CDS             11082..11699
                     /locus_tag="VYQ30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1965435.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSRYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            11749..12748
                     /locus_tag="VYQ30_000263"
                     /pseudo
     CDS             11749..12748
                     /locus_tag="VYQ30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            12875..13834
                     /locus_tag="VYQ30_000264"
     CDS             12875..13834
                     /locus_tag="VYQ30_000264"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1965436.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            13931..14770
                     /locus_tag="VYQ30_000265"
     CDS             13931..14770
                     /locus_tag="VYQ30_000265"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1965437.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(14818..14988)
                     /locus_tag="VYQ30_000266"
     CDS             complement(14818..14988)
                     /locus_tag="VYQ30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1965438.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            15109..15993
                     /gene="miaA"
                     /locus_tag="VYQ30_000267"
     CDS             15109..15993
                     /gene="miaA"
                     /locus_tag="VYQ30_000267"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1965439.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIYSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            15986..17224
                     /gene="hflX"
                     /locus_tag="VYQ30_000268"
     CDS             15986..17224
                     /gene="hflX"
                     /locus_tag="VYQ30_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1965440.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            17217..17840
                     /locus_tag="VYQ30_000269"
     CDS             17217..17840
                     /locus_tag="VYQ30_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1965441.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            17855..18784
                     /locus_tag="VYQ30_000270"
     CDS             17855..18784
                     /locus_tag="VYQ30_000270"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1965442.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            18805..19560
                     /locus_tag="VYQ30_000271"
     CDS             18805..19560
                     /locus_tag="VYQ30_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1965443.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLVQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(19614..20522)
                     /locus_tag="VYQ30_000272"
     CDS             complement(19614..20522)
                     /locus_tag="VYQ30_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1965444.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            20586..20855
                     /locus_tag="VYQ30_000273"
     CDS             20586..20855
                     /locus_tag="VYQ30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1965445.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            20855..21235
                     /locus_tag="VYQ30_000274"
     CDS             20855..21235
                     /locus_tag="VYQ30_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1965446.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            21353..21550
                     /locus_tag="VYQ30_000275"
     CDS             21353..21550
                     /locus_tag="VYQ30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965447.1"
                     /translation="MGILSIILGLLFPIVGLILGIIGLVLAISYQKESQLDYKIEKIL
                     NILGIVISVVNWIVAIALIFR"
     gene            complement(21589..22314)
                     /locus_tag="VYQ30_000276"
     CDS             complement(21589..22314)
                     /locus_tag="VYQ30_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1965448.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSK"
     gene            22476..24317
                     /gene="typA"
                     /locus_tag="VYQ30_000277"
     CDS             22476..24317
                     /gene="typA"
                     /locus_tag="VYQ30_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1965449.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            24337..24591
                     /locus_tag="VYQ30_000278"
     CDS             24337..24591
                     /locus_tag="VYQ30_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1965450.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         7627 a         4484 c         5608 g         6995 t
ORIGIN      
        1 cctcaaagct atactttgag ctgacttcgt cagttttatc tacaacctca aagctatact
       61 ttgagctgac ttcgtcagtt ttatctacaa cctcaaagcg gtactttgag aaacctgtgg
      121 ctatcttcct agtttacttt ttgattttta ttgagtataa gaggtctttc ctaagcagtt
      181 agaggagaat tttgctatac tgatactagt aagtggtaaa ggagcagatc atgacctaca
      241 caatcttaat cgtagaagat gaatatctgg taagacaagg tttgactaaa ctggtcaatg
      301 tagcagccta cgatatggaa atcatcggtc aggctgaaaa tggaaggcag gcttgggaat
      361 tgatccaaaa gcaggtgcca gatatcattt taaccgatat caacatgcct catctaaatg
      421 gcatccagtt ggccagtctg gtacgagaaa cctatcctca ggttcatttg gtctttttaa
      481 caggttacga tgattttgat tatgccttgt ctgctgtcaa actaggtgtg gacgactacc
      541 tgctcaaacc cttttctcgt caggatattg aggaaatgtt ggggaaaatc aaacaaaaac
      601 tagacaagga agagaaagaa gagcagttac aagatttatt gactaatagg tttgaaggaa
      661 acatggccca gaaaatccag tctcatctgg ctgatagcca atttagttta aagtctttag
      721 ccagtgactt aggttttagt ccgacctatc tgagttcctt gattaagaaa gagttgggct
      781 tgccttttca ggattatctg gtgagagaac gtgttaaaca agccaagctc ttgcttttaa
      841 ctacagatct gaagatttat gagatcgcag agaaggttgg ttttgaagat atgaactatt
      901 ttacccaacg ttttaagcag attgcaggtg tgacacctcg tcagtttaag aagggagaag
      961 accgatgaag cgttcttctc ttttagttag aatggttatt tccatctttc tggtctttct
     1021 cattctccta gctctggttg gaactttcta ctatcaatca agttcttcag ccattgaggc
     1081 caccattgag ggcaacagcc aaacgaccat cagccagact agccacttta ttcagtctta
     1141 tatcaaaaaa ctagaaacca cctcgactgg tttgacccag cagacggatg ttctggccta
     1201 tgctgagaat cccagtcaag acaaggtcga gggaatccga gatttgtttt tgaccatctt
     1261 gaagtcagat aaggacttga aaactgttgt gctggtgacc aaatctggtc aggtcatttc
     1321 tacagatgac agtgtgcaga tgaaaacttc ctctgatatg atggctgagg attggtacca
     1381 aaaggctatt catcagggag ctatgcctgt tttgactcca gctcgtaaat cagatagtca
     1441 gtgggtcatt tctgtcactc aagaacttgt tgatgcaaag ggagccaatc ttggtgtgct
     1501 tcgtttggat atttcttatg aaactctgga agcctatctc aatcaactcc agttggggca
     1561 gcagggcttt gccttcatta tcaatgaaaa ccatgaattt gtctaccatc ctcaacacac
     1621 agtttatagt tcgtctagca aaatggaggc tatgaaaccc tacatcgaga caggtcaggg
     1681 ttatactcct ggtcacaaat cctacgtcag tcaagagaag attgcaggaa ctgattggac
     1741 ggtacttggc gtgtcatcat tggaaaagtt agaccaggtt cggagtcagc tcttgtggac
     1801 cttgcttggg gccagtgtca catctcttct tgtctgtctc tgcttagtgt ggttcagtct
     1861 taaacgctgg attgctcctt tgaaggattt gagagaaacc atgttggaaa ttgcttctgg
     1921 tgctcaaaat cttcgtgcca aggaagttgg tgcctatgaa ctgagagaag taactcgcca
     1981 atttaatgcc atgttggatc agattgatca gttgatggta gctattcgta gccaggaaga
     2041 aacgacccgt cagtaccaac ttcaagccct ttcgagccag attaatccac atttcctcta
     2101 taacactttg gacaccatca tctggatggc tgaatttcat gatagtcagc gagtggtgca
     2161 ggtgaccaag tccttggcaa cctatttccg cttggcgctc aatcaaggca aggacttgat
     2221 ttgtctctct gacgaaatca atcatgtccg ccagtatctc tttatccaga aacaacgcta
     2281 tggagataag ctggaatacg aaattaatga aaatgttgcc tttgataatt tagtcttacc
     2341 caagctggtc ctacaacccc ttgtagaaaa tgctctttac catggcatta aggaaaagga
     2401 aggtcagggc catattaaac tttctgtcca gaaacaggat tcgggattgg tcatccgtat
     2461 tgaggatgat ggcgttggct tccaagatgc tggtgatagt agtcaaagtc aactcaaacg
     2521 tgggggagtt ggtcttcaaa atgtcgatca acggctcaaa cttcattttg gagccaatta
     2581 ccagatgaag attgattcta gaccccaaaa agggacgaaa gttgaaatat atataaatag
     2641 aatagaaact agctaactcc cagtcaattc tgggagtttt atgcgtaatt gggcaaactt
     2701 tataggttgt ctatcttgct aaaaacaaga aaaaacggta tgatagaaga gtgacaacag
     2761 aggtaacaga gatgaacgag acacagattc aaagagaaac acgtcaggta gtggaagatg
     2821 ttttagaaaa gaccaatttg aagcaggggg tcctttttgt tttaggtcta tcttctagtg
     2881 aggtgctagg tggtcagatt gggaaagaat ccagtcaaga gattggtgag ctgatagtgg
     2941 agactatctt gggtattcta ggtagtagag gtatccattt agcagttcaa ggttgtgagc
     3001 atgtcaatcg ggctctcgtt gttgaacgtc aggtggcaga gcagtttgat ctggaaattg
     3061 tcagtgtcca tcctactctt catgcaggag gttcggggca gttggcagcc tttaagttta
     3121 tgcaggatcc agttgaggtt gaatttatca aggctcatgc tggattggat atcggagaca
     3181 ctgcaattgg catgcatgtc aagcatgttc aggttccgat tcgccctatt ttgagagaga
     3241 ttggtcatgc ccatgtaacg gcactggcta gtcgtccaaa attaatcgga ggtgcgcgtg
     3301 cgcactatcc gcaagacgct attagaaagt cttgagaatg aataaaggag ataaaagtgt
     3361 ttaaaaaaga ccgtttttca attcgtaaga ttaagggagt tgtaggctct gtatttcttg
     3421 gaagcctttt gatggctcct tctgtagtgg acgcagccac ctatcactat gtaaataaag
     3481 agattatttc acaagaagct aaagatttaa ttcagacagg aaagcctgac aggaatgaag
     3541 ttgtatatgg tttggtgtat caaaaagatc agttgcctca aacagggaca gaagcatctg
     3601 ttttgacagc ttttggtttg ctgactgttg ggagcttgct tttaatctac aagagaaaga
     3661 aaattgctag cgtctttcta gttggagcta tgggattggt agttcttcct agtgcagagg
     3721 ctgtagaccc agttgcgacc ctagcgctgg ctagtcgaga gggtgttgtt gaaatggatg
     3781 gctatcgcta tgttggttat ctatcaggtg acatcctcaa aacgcttggc ttggacactg
     3841 ttttagaaga aacctcagca aaacctggag aggtgactgt agtcgaagtt gagactcctc
     3901 aatcaacaac aaatcaggag caagctagga cagaaaacca agtagtagag acagaggaag
     3961 ctccaaaaga agaagcacct aaaacagaag aaagtccaaa ggaagaacca aaatcggagg
     4021 taaaacctac tgacgacacc cttcctaaag tagaagaggg gaaagaagat tcagcagaac
     4081 cagctccagt tgaagaagta ggtggagaag ttgagtcaaa atcagaggaa aaagtagcag
     4141 ttaagccaga aagtcaacca tcagacaaac cagctgagga atcaaaagtt gaacaagcag
     4201 gtgaaccagt cgcgccaaga gaagacgaaa aggcaccagt cgagccagaa aagcaaccag
     4261 aagctcctga agaagagaag gctgtagagg aaacaccgaa acaagaagat acacagccag
     4321 aagtagtaga aacaaaagat gaggctgcta atcaacctgt tgaagaacca aaagttgaaa
     4381 cgcctgctgt agaaaaacaa acggaaccaa cagaggaacc aaaagttgaa caagtaggtg
     4441 aaccagtcgc gccaagagaa gacgaaaagg caccagtctc tccagaaaag caaccagaag
     4501 ctcctgaaga agagaagact gcggaagaaa caccgaaaca agaagataaa ataaagggta
     4561 ttggtactaa agaaccagtt gataaaagtg agttaaataa tcaaattgat aaagctagtt
     4621 cagtttctcc tactgattat tctacagcaa gttacaatgc tcttggacct gttttagaaa
     4681 ctgcaaaagg tgtctatgct tcagagcctg taaaacagcc tgaggtaaat agcgagacaa
     4741 aagctgaaaa agttgcagct aatacagatg ctaaacaaag tgaagtaaat tctgagactg
     4801 caagtcttaa aacagcaatt tctggattaa atacagataa agttgaactc gaaaatcaac
     4861 ttaagattgc acagggaaaa actgaaactg atttcagcat ggaatcttgg acggttttga
     4921 gtacagctaa aaataaagct caggaagtaa aagataacgg aacagcgacc caagaacaaa
     4981 taaacgaagc agagaaaagt ttgaaaacag cccttgctga cttatctgtt gacaaaacag
     5041 ctttgggaag cgctatcgat acagctacca agaaaaataa agagaactat acaaatcaaa
     5101 catgggcaga gttagaaaca gttcttacag cagcaaaatc tgtaaacact aacgagtcta
     5161 aacaaagtga agttaatgaa gctgttgaaa aattaactgc aactattgaa aaattggttg
     5221 aattgtctga aaagccacgt ttgactctat ctattgaaaa gagagacata gatcgaaaag
     5281 ttacggtcac gtatactttg gaaaatccag caaatactca aataaaatca ataactgcaa
     5341 ctctgaaaaa aggagaggaa gttgttaagg attttgtttt aacagaagaa aatcttaaaa
     5401 ctaatcattt aactgctttg tttgaaaagc tagattacta caaagaatac actctatcta
     5461 cggatatggt atataacaga ggaaatgatg atgagacgga gagtatttca gaagaactaa
     5521 ttcagctgaa tcttaagaaa ctagaattga aagatatcca gacagtctct ctgatgaaat
     5581 ttgaaaatgg tcaagaaagt caggttacac acttgtctga taaaccgacc gatttgagta
     5641 aattatactt aaaagttaca tcatcaacgt ctaaggatgc tgtgttagcc gttagctcta
     5701 tagaagaaga gattgttgaa aataaaaaaa tctttaaaat tcatgcagat acaccagaat
     5761 tagtagttcg taagaaagat ggcagtcttt ctaagggatt tgactattac atggagagag
     5821 ttatccctca tgatggtgat atttattatg attttaaaga cttgatttca gctatgacat
     5881 ctaatcctac aggtacgttt attttaggga gagatatttc ctcgcgtaat gtaaaaccgg
     5941 atggaaatgg aaaatcttat atcaaaggtg aatttaaagg aaaactactt ggtacaaatg
     6001 ataatgttag acactcgatc tttgacttag aatatccatt gtttgatact ataaaatcag
     6061 gagtcgtgaa agatattgat ttcaagcatg tcaatatggt attcccagat agcaatcaag
     6121 gagataacgt agcaacaatt gcaagagtaa tcaaagataa gacaaaaatt gagaatgtta
     6181 atgttgaagg ttatctcgag ggtcgagatc atgtagctgg tttagttaat aatcttgaag
     6241 gtaattccga aattgaaaat atatccttta caggaaagat taagtctaaa ggtggtaatt
     6301 ctattacagc aggtattgca ggtagaaata tcttatctcg tgttaaacgt gcctatgtaa
     6361 atgcaaatat tgaagtcctt ggttcaacga attcgagtat gcttgttgca gtaaatggta
     6421 caacattgaa tgctagtgga ggttggggag cctggggaag acttactgag tccgttgcta
     6481 aagggacgtt agaaattaaa cgcagtggac aagctggtgg agttactgca acagtctggc
     6541 cttatggagc aattgataag gttgtcagct atgcaaaagt taccaaagga aaagaattat
     6601 ttgggtcaga tggtgattta aataataatt ggtttatgca aaaaattaat aatatttttg
     6661 gtgttcaagg tatcagctca ggtgactcag ggaatgattc taagtttaag cgtatttccg
     6721 aggaagaagc aaagcaaaag gttgcctctt ataatattac agctcccaac ttgatgagcg
     6781 actcaagtct tttggtagac agactcaatg aaagttggaa aaatactgat caatttgaaa
     6841 gtatacagga ttatcaatcc caaaaccaat taatttacca gaacttgact aaatttactc
     6901 cttattacaa taaagaattt attgttcatg aaggaaatgc tctcactcct gaacaggaaa
     6961 tattgaagac taagaaaatc aaatcgattg tagggttgaa aggaacagag ttcgttgtag
     7021 atggcagtga tattgatact atcatgcttc attttgagga tggtagtcag aagcgatata
     7081 aggtaacctc tactggtaag ttttcgataa ctaatttacc ggaatatcag gttgaagatt
     7141 taaatgtggt gtatacatca gaacatatag tacatccact agattcgtca ctgataaata
     7201 accttgttga agaattgaaa aaagtagaat tgtatacaga gtctacttac caagttctag
     7261 gtattgataa agataatgct aacaaattga atagaactaa gagattattc ttagatgagt
     7321 cattagatgc tgttaaaaca caattgccta cctttgtaaa aacaatgttt gaaaatgagt
     7381 ggttgcacat caatggagaa tctagtggag ctgtcgctgc tttacgacaa aaaatcatgg
     7441 ataataagac ggctatattg cttgctttga cttatatcaa tcgttattat gatgttaaat
     7501 tctcagacta taatattaaa aaactgatgc tctttaaacc aactttccat ggtgaaaaga
     7561 ttgatctatt agatcgatta atccgactag gatcatctgg agaaaataga ttaaaaggtt
     7621 cagaaaatgc agaaacgttc aagcagttat ttgctagcga aacgaagcag aaggatttgg
     7681 tgacttattt agactacaat cgctctttgt tgactaatta tcaaacaaca ggggaatggt
     7741 ttaaagaaac gactaaggat tatattcaat ttgaggaacg tccatcctta gtagaggaga
     7801 ttaaagatgc caaatatcga gtttacgata atttaaccgc accgtattat caaggatata
     7861 tcctacctct cttaacgttg aagaatactc atttagcaat tttatcgaac tatagtacta
     7921 tgacatttgt cagccgtgaa aaacgcccta attggaaaaa tgaagatttt gataagtggg
     7981 tgaaatatgt agcaactgct catagaaacc atgttgatac ttggtataag attttacctg
     8041 ataatatcaa aggtaaaatg gttaaagaga atgttactgc tgtttgggaa ggattgagca
     8101 tcccaggaag tgaatgggta gaccagaatg ctgtagatag aaaaggaaga gattacgccc
     8161 cagctcgaga attctttaat cttgtaggtg gtcctatggg aggttggtat gcttaccatg
     8221 gttatggtgc gcatgctgga ggaagaaatc gggttaatta tgaggtgttt gatgtattaa
     8281 gtgagtatgg tatatctgtc ttcactcatg aattgactca cgttaatgat acttggattt
     8341 acttaggagg gtatggtaga cgtgaaaata tgggacctga agcttatgct cagggattat
     8401 tccagtctcc tgttccaggt cagccgggtt ggggggcact gggtctcaat atggcatttg
     8461 aaagaaaaaa tgatggtgat ctaatttata atgcctcccc aactcaattt gaaaatagga
     8521 aagagttaga tagttatatg aaaaattata acgatactct catgatggtg gactacttgg
     8581 aaggagatgc agttatctct aaaggtaaag aagctattac taaatggttt aaaaaagttg
     8641 aacctaaggt tgttagtcaa acagctcagt atgatacggt tcgtcagttg actgctgaag
     8701 agaaagaaaa actttctgtt tctagtgttg atgatttagt tgatcaagga ttaatgtcag
     8761 acagagctgt aggaaataat acctataatc cagctgattt tgaaacaagt tatattgcga
     8821 tagattatat gactggtatt tatggtggag gaaagaatag tgtaggttca ccaggtgcct
     8881 tgatgtttaa acataatact ttccgtatgt ggggctacta tggttttgaa gaaggtgttc
     8941 taggatatgc ttctaataaa tttaagcaag catctagaga tgaaggtcat gctggactta
     9001 gtgataactt tattatttct aagatttcca aaggggaatt tttaactatg gaagcattta
     9061 aaaagggata ttttaagaaa gtagtagaag agttaaaaac taaaggtatt cgtcctgtta
     9121 ctatcaatca aaaaacatat agcacctttg aagaattgca agaaggattt aagcaggctg
     9181 ttgaaagaga tttgaagaaa aatcagttag atgagaggga aacaagaaac ttcaaattcc
     9241 aagttttcag acaacttctt caacaaacag atagctttaa aacttcaatc tttagataat
     9301 cgaaatctct catctgcttt gcggatgagg gttttcttat tttatgctat acttaaggta
     9361 tgcatagaaa aacagtgatt gattttagga ctttagggga gagatatacc tttacccagc
     9421 ctattaaaga gttgaaaacg agaaatgtag aagaagtggc agatttgctg gcacaagtgg
     9481 aaagctacca agagcaaggt tattatgtgg tggggtatgt cagctacgag gctgcacctg
     9541 cttttgagga gaaattaaca gttcacaagg ttcctctact gggcgagtac ttgctttact
     9601 ttactgttca cgatagggta gagacctccc ctgttcctct gacttatgag gatattgatt
     9661 tgccctcaaa ttggcaggaa gtgacgtctg cagcagacta tgaaaaggcc attgcccaga
     9721 tacaccatca cttgaggcag ggagacacct atcaggtcaa ctacaccgtc caactcaagc
     9781 aaaagttaag tgccaatcct tttgccatct acaatcgtat ggtggtagag caggaggcgg
     9841 gctacaatgc ctacgtggaa catgacgaga tggcagtgat ttccatgagc ccagagctct
     9901 tttttgagca aaatgatcgc gagttgacaa cacgaccaat gaaggggacg actcagcgtg
     9961 gggtaactga ccaagaagat cttgaacagg ccagttggtt ggaacaggat cccaaaaatc
    10021 gctctgaaaa tatgatgatt gtggacctct tgcgcaatga tatgaaccgt atttctgaag
    10081 ttgggagcga gcacgtggag cgtctgtgtc aggtagagca gtattcaact gtttggcaga
    10141 tgacttcgac catcaagagt cagttgcgag aggatgtgga ccttgttgaa atcttccgct
    10201 cactctttcc ttgtggttcc ataacgggtg caccgaaaat tgcgacaatg gagattatca
    10261 aggacttgga gcctcaaccg cgtggagtct actgtggaac gattggtctc ttgcttccaa
    10321 atggacgacg gatttttaat gtggccattc gtaccattca acttcacaaa ggtcaagcca
    10381 tttatggagt tggcggaggg attacttggg atagcacatg ggaatctgaa taccgagagg
    10441 ttcatcaaaa ggcagcagtt ctttatcgta aacaagctcg cttccaactg attacgacag
    10501 gggaaatcag ccagaagaac ctgctgtttg aagatcaaca tctggaaaga ctgagaaaag
    10561 ctagtcgtta ttttgctttt ccttttgatg cagaagactt gggacacaag attgaggaag
    10621 agtgtcagga ttgtgaagct aatcaagatt accgcttgcg aatcagcctt agcaaatctg
    10681 gagagataga agtcaatcgt caagtattaa cccctctcag tacaagcttt tgtcaggccc
    10741 aagtctgtct tcaggaagct gctttgaatc aatcctttac ctactttaaa accactcacc
    10801 gaccgcattt gagcctagga gaacaagaga agatttacca caataagtca ggagaactgc
    10861 ttgaagcctc tataggaaat ttggttctga aaatcgctgg aaaactctac acaccgccta
    10921 tccgacttgg aatcttgcca ggaatttacc gtcagtattt gctagaaaca ggacaggtag
    10981 aagagaaagt cttgaccttg gcagatttag ctcaagcaga agctatttac ggctgtaatg
    11041 cagtgagagg cttgtatgaa ctaagccttg aggagaacta gatgaaattg atatttttgc
    11101 atgggttagg gcagtcagca gagtcttgga aagaagttcg gaatctactg acagattatc
    11161 cttctgaggc tatcgagtta tttccttctg gagttagtaa ctatcaacaa gctaaagagc
    11221 gagtttatca gcacctagct caagagacag aaccttttgt tttgattgga ttgtccttag
    11281 gtgctgccct gacactagaa ctttccagat acgatttacc aaatcttcga gctttgattc
    11341 tgtcaggatg cccgcttaaa ttagcaggga atatcctatt ttatcttcag ttgctgattt
    11401 ttaaactact tcccaaaaga gtatttgaaa aacagggagc agacaaggct cttatggtcg
    11461 gagtttctga ggaattgaag acacttgatt taacggatat agcaggaact tgcccttatc
    11521 caaccttact aatttgtggc agcaaagata aaccgaatct tagttctatg aaagctctcc
    11581 atagactgtt aacagattcg cagtttcaga tcattcctga tggccctcat gtcttgaata
    11641 aggcaaaacc aaaggagttt gtagaaaaga ccagaagttt ccttgaattg ctgaaataag
    11701 tttgataaaa taatattgaa atcgatatca taattatggg agaaagaaat gaataaacgt
    11761 ctatttttaa aaatgagtct ggtgacgttg ccaattttag ccttgttttc acaaccagtt
    11821 ttggcggaag aaaacatcca tttttcgagc tgtaaggaag cttgggcgaa tggctattcg
    11881 gatattcacg agggggaacc tggttattct gccaagttag accgtgatca tgatggtgtg
    11941 gcttgcgaat tgaaaaatgc tcctaagggt gcttttaaag caaaacagtc aacggctatt
    12001 caaatcaaca caagttcagc aacaacaagt ggttgggtta agcaggacgg cgcttggtac
    12061 tactttgatg gaaatggaaa tctagtgaaa aatgcatggc agggaagcta ttacctgaaa
    12121 gctgatggta aaatggcaca gagtgaatgg atttatgact cttcttatca agcttggtat
    12181 tatttgaaat cagatggttc ttatgcaaaa aatgcatggc aaggagctta ttaccttaaa
    12241 tcaaacggta aaatggcaca aggtgagtgg gtttatgatt cttcttacca agcatggtat
    12301 tacttgaaat cagatggttc atatgctcgc aatgcatggc aaggaaacta ctatttgaaa
    12361 tcagatggta aaatggctaa aggtgaatgg gtttatgatg ccacctatca agcttggtat
    12421 tatttgacat cagatggttc ttatgcttac agtacatggc aaggaaatta ctatctaaaa
    12481 tcggatggta aaatggctgt caatgaatgg gttgatggtg gacgttatta tgttggcgct
    12541 gacggagttt ggaaggaagg tcaagcaagt acagcttctt ctagtaatga tagcaatagt
    12601 gaatattctg ctgctttagg aaaggcaaaa agttataatt cgttattcca catgtcaaaa
    12661 aaaacgtatg tatagacaat taacttctga ttttgataaa ttttcaaatg atgcagctca
    12721 atatgccatt gatcatttag atgattaaca gatactggta ggcgaaaaaa ttccttataa
    12781 ttcacttacg caaacctttg cataagtgaa tgaattttga tatactagta ctgtaagcat
    12841 tttcaattac tacacaatga aaaaggagaa tatgatgagt caaaagatta ttgggattga
    12901 ccttggtgga acttctatca aatttgcaat cttaacaaca gcaggagaaa tccaaggaaa
    12961 atggtcaatc aagacaaaca ttttggatga gggaagtcat atcgttgatg atatgattga
    13021 gtctattcaa catcgtttgg acttgcttgg attggcagca gcggacttcc aaggcattgg
    13081 aatgggatca ccaggtgtgg ttgaccgtga aaaagggact gttatcggtg cctacaactt
    13141 gaactggaaa acccttcaac caatcaaaca aaagattgaa aaagctttgg gcattccatt
    13201 tttcatcgat aatgatgcca acgtagcagc tcttggtgag cgctggatgg gtgctggaga
    13261 taaccaacca gacgttgtct ttatgacact cggtactggt gttggtggcg gtatcgtcgc
    13321 agaaggcaaa ttgcttcacg gtgttgctgg tgcagcaggt gagcttggtc acatcactgt
    13381 tgactttgac cagccaatct catgtacttg tggtaaaaaa ggctgccttg agacagtcgc
    13441 ttctgcaaca gggattgtca acttgactcg tcgctatgcc gatgaatacg aaggcgatgc
    13501 agccttgaaa cgcttgattg ataacggaga agaagtaact gctaagactg tctttgatct
    13561 cgcaaaagaa ggagacgacc ttgctttgat tgtttaccgt aacttctcac gttacttggg
    13621 aatcgcttgt gctaacattg gttcaatcct aaacccatca acaatcgtca tcggtggtgg
    13681 tgtgtcagct gcgggagaat tccttctaca aggtgttcaa aaagtttacg atgaaaatag
    13741 tttcccacaa gtacgcacat ctactaaatt agctcttgca actctaggaa atgacgctgg
    13801 agttatcgga gcagcatcac ttgtattgca ataagataat gagagaggaa aatgctaacc
    13861 tagagttagt aatgttcctc ttttattttt tatgctatac tagttaggac aatgaatgag
    13921 gtgagagtaa atgacaaaag cagatacgat ttttaaagag aatattgaac gcattctaaa
    13981 ggaaggtgtt ttctcagagc aggctcgtcc taagtacaag gatgggactg ttgctaattc
    14041 caagtatgta actggtgcct tttccgagta cgacttatct aaaggagaat ttcctatcac
    14101 aaccttgcgt cccattgcaa tcaaatccgc catcaaagaa gttctctgga tttaccaaga
    14161 ccagtcaaat agcttagaag tgcttaatga caagtacaat gttcactact ggaatgactg
    14221 ggaagttgga gacacgggaa ccattggtga gcgctatggt gccgttgtta agaaacacga
    14281 cattatcaat aagcttctca aacagttgga aaccaatcct tggaaccgcc gcaatattat
    14341 ttcgctctgg gattaccaag ctttcgaaga aacagatggg ctgctcccgt gcgcctttca
    14401 gaccatgttt gatgttcggc gtgttgatgg ggaaatctat ctggatgcga ccttgaccca
    14461 gcgctccaat gatatgctgg tggcccacca catcaacgct atgcagtatg tggctttgca
    14521 gatgatgatt gctaaacatt ttggctggaa ggttgggaag ttcttctact tcatcaacaa
    14581 cctccatatc tatgataatc aatttgaaca agctcaggaa ttgctccgtc gggagccgtc
    14641 aaactgccaa ccacgcttgg ttttaaatgt tcctgatggg actaatttct ttgatatcaa
    14701 agcagaagat tttgagttgg tggattatga ccctgttaag ccacagttga agtttgacct
    14761 agctatttaa aagaatagaa aaaagaagtt gagaataatc ccaacttctt ttgtttctta
    14821 acgtgatacg cggcgacgag ctgctttttt acggttttct tcgatgaaag ctgctttttg
    14881 ctcttctggt tcgattactt tctttttaaa tgcgtatact gcacctgcaa cggcagcgac
    14941 agttcctgcg acacctgtta caagaccttt agcgaatcct ttagccatga gtcttcctcc
    15001 tttatattct caatcagcca gcctcctcaa gaggtcacat ttttctgact gacctttttg
    15061 tgttataata atagtaacga aaaaatggga atttttcaag gaaaaaagat gaaaacaaaa
    15121 ataattgtga ttgttggacc gactgctgtt ggaaagacgg ctctagctat tgaagttgca
    15181 aagcgcttta atggcgaagt ggttagtgga gatagccagc aagtctatcg aggacttgat
    15241 attgggacgg ccaaggctag tccagaagag caggcagctg ttcctcatca tttaatcgat
    15301 gttagagaga taaccgagtc ttactcggct tttgattttg tttcagaagc taagatgact
    15361 attgaggata tttacagccg tggcaagcta gccattatcg ccggtgggac tggactttat
    15421 atccagagct tgctagaagg ttaccaccta ggtggggaga ctcctcatga ggagatttta
    15481 gcttatcgag ctagtttgga gccatattca gatgaggaat tagcccattt ggtggagcaa
    15541 gcaggccttg agattcccca gtttaatcgt cgtcgtgcta tgcgtgcctt agaaattgcc
    15601 cattttggtc aggatttgga aaatcaagag attttgtatg aaccgctgat tatctgcttg
    15661 gatgatgaac gtagtcaact ttatgagcgt atcaaccacc gagtggattt gatgtttgag
    15721 gctgggcttt tggatgaggc caagtggctg tttgaccatt cccctaatgt acaggctgct
    15781 aaaggaattg gctataagga actctttcct tatttccgtg gagagcagac ctttgaggaa
    15841 gctcgtgaga gtcttaaaca ggcgacccgt cgttttgcca agcgtcagct gacctggttc
    15901 cgtaatcgca tgcaggtcac cttttatcag attggagaat ctggtgtaca agaccgcatt
    15961 ttaagccaga tagaggagtt tttagatgat tgaaacggag aaaaaagagg agcgagtcct
    16021 gctgattggt gtggaattgc agggtatgga cagttttgac ctctccatgg aagaattggc
    16081 tagtttagcg aaaacggcag gggcagtcgt tgtagatagc tacagacaaa aacgtgaaaa
    16141 atatgattcc aagaccttcg tcggctctgg taagttggaa gagattgcgc ttatggtgga
    16201 tgcagaagaa atcactactg tcatcgtcaa caaccgtctg accccaaggc agaatgtcaa
    16261 tctagaggaa gttctgggtg ttaaggtcat tgaccgtatg cagttgattt tggatatctt
    16321 tgccatgcgg gctcgaagcc atgaagggaa gctccaagtc cacctagccc aactcaaata
    16381 cctcttgcct cgcttggttg gtcaggggat tatgctcagc cgtcaggcag ggggaattgg
    16441 ttcccgtggt cctggtgaaa gccaactgga gctgaaccgt cgtagcgttc gcaatcaaat
    16501 cacggatatc gagcgccagc tcaaggtggt tgagaaaaat cgggcgactg tcagagaaaa
    16561 acgtttggag tctagcactt ttaagattgg tttgattggt tataccaatg ctgggaaatc
    16621 aactatcatg aacatcttga ccagtaagac ccagtatgaa gcagatgagc tctttgcgac
    16681 tctggatgcg acaaccaaga gtattcatct gggaggcaat ctccaagtaa ctttgacaga
    16741 taccgttggc tttatccaag atttgccgac agagttggtg tccagtttca agtcaacctt
    16801 ggaagaaagc aagcatgtgg accttctggt tcatgttatc gatgctagca atccttacca
    16861 cgaggagcat gaaaaaacgg ttctctccat catgaaagac ctggacatgg aagatattcc
    16921 tcacttgacg ctttataata aagcggattt ggtggaggat ttcacgccta cccaaacgcc
    16981 atataccctc atttctgcca agtctgagga cagtcgtgaa aacttgcaag cattattgct
    17041 agataagatt aaggaaattt ttgaagcatt taccctgcga gtgccttttt caaagtccta
    17101 caagattcat gatttagaga gtgttgcaat tctggaagaa cgtgattatc aggaagacgg
    17161 cgaagtgatt acaggctaca tttccgagaa aaataaatgg aggttagaag aattttatga
    17221 ctgatattaa aactttggct ctaaagtatg gaggttatac aagtctggac aaggtctatc
    17281 tggatcagct tctagctggt aaaacagagc aggagcagtt aacacttatt acacctccgc
    17341 caagtgttgt taatgcctac tttgcagaac tctatcagaa aaaaagtcct caggctgcga
    17401 cggattattt tgcagaactc agtcaggaac tgaatcttta caatactgag ccaagtttca
    17461 ccctagaaaa taagcctttt attcggctta atctgtctgg taaatccttt ggtttttgtt
    17521 atgagagtga cggctttggt cggattttct ctgaaaatga ggagaaaatc tcggatgact
    17581 tgctctttga aattgcgcaa attttctccc atcaattagt ctttgaagag tctggtaaga
    17641 tttacatgaa gcctgtcaag gacgaggaag ttgtcagcct agaaagtctc acagctttga
    17701 ctgatttgga aagattggct gatggtcgaa aacgtctcaa aggctacagc caagaggatt
    17761 tattgcaaga agctgctgct ttttctggca aacgctattt ccgatcggaa aaccgcacag
    17821 ccatgttata tattgattaa ttagaaagta tcgaatggat attcaatttt taggaacggg
    17881 ggctggtcag ccctctaaag cccgcaacgt ttcaagtctc gccctgaaac ttttggacga
    17941 gattaacgaa gtttggctct ttgactgtgg agaaggtacg caaaatcgca ttctggaaac
    18001 cacaattcga ccacgtaagg tcagcaaaat ctttattact catctgcatg gagaccacat
    18061 ttttggtttg ccaggtttcc tttctagccg tgcctttcag gccaatgaag agcagacaga
    18121 tttggaaatc tacggacctc aaggaatcaa gtcatttgtc ttaaccagcc ttcgtgtgtc
    18181 aggttctcgt ctgccctacc gcattcattt ccatgagttt gaccaagatt ctctgggtaa
    18241 aattcttgaa accgataaat tcactgtgta tgcagaggag ctggaccaca ctattttctg
    18301 tgttggctat cgtgtcatgc aaaaggatct agaagggacg ctggatgctg aaaaactcaa
    18361 ggctgctggt gttccgttcg gcccgctttt tggtaaaatc aaaaacggcc aggatcttgt
    18421 tttggaagac ggaactgaaa tcaaggcagc agactatatc tcagcgccac gtccaggtaa
    18481 gattatcact attttaggag atactcgaaa aacggatgcc agtgtgcgtc tggctgtcaa
    18541 tgcagatgtc ctagttcatg agtccactta tggcaagggt gatgaaaaaa ttgctcgtaa
    18601 ccatggtcac tcaactaata tgcaagctgc acaagtagcg gtagaagcag gtgccaaacg
    18661 cctcctactc aaccatatca gtgcccgttt cctctcaaaa gatattagca aactcaagaa
    18721 agacgctgcc acaatttttg aaaatgtcca tgtggtcaaa gacttggaag aagtggaaat
    18781 ctagcagtca cagaaaggat aagtatgcct actattctca ttaccggagc tagcggtggt
    18841 ctagctcaag aaatggtcaa actcctgccc aatgaccaac tcatcttgct tggtagaaat
    18901 aaagaaaaat tagtccaact ctacggaaat tattcccatg cagaattgat tgaaattgat
    18961 attaccgacg attcagccct agaagctctg gtaactgatc tttatctccg ctatggcaag
    19021 attgatgtct tgattaacaa cgctggttac gggatttttg agggatttga ccaaattgct
    19081 gataaagaca ttcaccagat gtttgaggtc aatacctttg ccctgatgaa tctgtctcgt
    19141 caccttgcgg ctcgtatgaa ggaaagcagc aaagggcata tcatcaacat cgtcagcatg
    19201 gcaggtctaa tagctactgg caagtctagt ctttactcag cgaccaagtt tgcggctatt
    19261 ggtttttcaa atgctctgcg actcgaactt atgccctatg gagtctatgt gacaacagtc
    19321 aatccaggtc caatccgaac aggatttttt gaccaagctg acccagatgg aacttatctt
    19381 aaatcggttg accgcttcct cttagaggca gatgcagtgg ctaaaaagat tgtcaagatt
    19441 ataggcaaaa ataaacgaga actcaatctc ccgattttgt tgaacctagc ccataagttt
    19501 tatactctct ttcccaagct agctgataag ttggcagggg aaacttttaa ttataagtaa
    19561 aaagaaccaa tgtgcaggtt gttgctagcc tacatattgg ttcttatcat ttctcaacta
    19621 tcaaactgaa cttcttctag tagatagtcg ataaagcgtt cgcccatctt agataggctg
    19681 gttttctcat gctggatata gaccagctca atcgggtcat caatatccag tgggatagaa
    19741 acgatattgt ctccgtttag gttgctgttc aaaatacctg tcgcaatggt ataaccatcc
    19801 aaaccaatca agagattaaa gagggtggca cggtcactga ccacaatgga tttcttgtgg
    19861 tgttcttgag aaagaatctc ttctgaaaag tagaaggagt tgtgcgtccc ttggtcatag
    19921 ctgaggtaag ggaaattctc caaatcagac agtttcacct tgtctttctt tgccagaggg
    19981 ttggtcttgc tgacaaagat atgtggttgc gctgtgaaga gatggtgggc tagcaggtga
    20041 ttgtcatcca gcatcttggt taaaacatca cggttgtaac tgtttaagaa gaggaccccg
    20101 acctcactgc ggaagttctt gacgtcgtcg ataatctccc aagtccgagt ttcacgaagg
    20161 aagagttcgt atttctccat atcgcttttc ttgagcaaag agacaaaggc attgaccaca
    20221 aaggcatagt gttgagacga aacgctaaat agttcgcggt gggcgacagg atttttatag
    20281 cgttcctcca gaagctgggt ctgctcgaca acctgacggg cataggagag aaactccatc
    20341 ccatcacggg tcaaggtgat tcccttggga ttgcggataa agatctcaat gcccatttca
    20401 ttttccaaat ctcgcactgc attggagaga cttggctgag tgataaagag ttgcttggct
    20461 gcctcattca tggagccagt ttcgacgatt ttgataatat aatgtaattg ttgaattctc
    20521 atgtttttat tgtaccatac ttgccttaga atcgctaaag aagatgggaa aataaggagc
    20581 attgtgtgag tctcttcttg aaaaaaacac aaaattttgc tagaatgagt cttatgaaaa
    20641 cattctatga tgtgcagcaa ttcctcaaac gatttggtat tattgtttac atgggaaaac
    20701 gcttatatga tattgaactg atgaagttgg aactatctcg gatttacgat gcagggttga
    20761 tggataaatt agactatcta gaggcagaag cagttcttcg cagagagcac aaggtagaat
    20821 tggattatat tgagaaaaat ggagaaaaga actaatggtt acttggattt tgtgggcact
    20881 tatactagca atgttggcgt ggatgggctt taactatctt cgtattcgcc gtgcggctaa
    20941 aattgtggac aatgaggagt ttgaagcctt gattcgtacg ggtcaattga ttgatttgcg
    21001 cgacccagca gaattccaca gaaaacatat ccttggtgca cgcaatattc cttcaagtca
    21061 gttgaaaact agtcttgcag cccttcgtaa agataaacct gtccttctct acgaaaacca
    21121 acgtgcgcaa cgagttacaa atgcagctct ttacttgaaa aaacaaggtt tttctgagat
    21181 ttatatcctt tcttatggct tggattcttg gaaagggaaa gtgaagacta gctaacctta
    21241 tttccaaatt agttgaatga taggatgaag ctattgtttt atagtataat taatttgtat
    21301 aaaatttaag gagttcttag aacatgaaag aaaagaagaa aatccacttt ttgtgggtat
    21361 cttgtcgatt attcttggtt tgttatttcc aattgtaggc ctgattttgg gtatcatagg
    21421 attggtcttg gctatttcat accaaaaaga gtcacaatta gactataaaa tagaaaagat
    21481 tcttaatatc ttaggaattg tgatttctgt agttaactgg attgtagcaa tcgccttaat
    21541 ttttcggtaa ataaaagaaa aaagctttag ttttaaagct tttttctttt atttactcat
    21601 ctctaaatag tttttaatga tttgtaactc tttttggttc aaagggcggt agttactttc
    21661 tgctaaatct gggttcaatg tgaagtcccc aaattggatt cttttgaggc tagtcacctt
    21721 aacaccaacc gagaggaaca ttttcttgat ttgatgaaat tttccttctg aaatggtgat
    21781 agaggcttga ctgagggagg gacttgcaga tagaatctct agttttgcgg gtttacagac
    21841 agtaccatct aaaaagacaa ttcccttttg aaaggcttgg atatggtcag gtgttagaag
    21901 tccattaacc tggacttggt aagtcttatc gacatgatat tggggatgga ggagctgaaa
    21961 gcccaaggga ccgttatcgg tcaagaggag gagtcccgtt gtatctcggt ccagtcggcc
    22021 aacggcatag agcttgtcag actggatgtc agatggaagc aggtccatga cggtcggaag
    22081 ttccttgtct ttgttggctg taacgacacc agcaggctta tgaagcataa gataggtgtg
    22141 ttcatagcct tgaatgattc gatcctgaaa aaggagttct tgtagtcctg tatcgatatt
    22201 ttgagctagg gatcgggctg ggcaaccatc gactagaatt tcccctctga ggagtgcttg
    22261 cttcatggcc tttcggctaa ctttttcttg ggctaataaa ttatctaaac gcatactgtg
    22321 cctatcttat cataagtcgc ttgctttgaa aaggcttgac gtgaaaagaa aagcagagtg
    22381 aaaacattta cacttgcttg agttatgtta tttacttgaa attatggtat aatcgttcag
    22441 ttagaaaata aattttgaat attatagagg aaatcatgac aaaattaaga gaagatatcc
    22501 gtaacattgc gattatcgcc cacgttgacc acggtaaaac aaccctggtt gacgaattat
    22561 tgaaacaatc agaaacgctt gatgcacgta ctgaattggc agagcgtgct atggactcaa
    22621 acgatatcga aaaagagcgt gggattacca tccttgctaa aaatactgcc gttgcttaca
    22681 acggaactcg tatcaacatt atggacacac caggacacgc ggactttggt ggagaagttg
    22741 agcgtatcat gaaaatggtt gacggtgttg tcttggtcgt agatgcctat gaaggaacca
    22801 tgccacaaac tcgtttcgta ttgaaaaaag ccttggaaca agaccttgtc ccaatcgtgg
    22861 ttgttaacaa aatcgataag ccatcagctc gtccagcaga agtagtggat gaagtcttgg
    22921 aacttttcat cgagcttggt gcagatgacg accagcttga tttcccagtg gtttatgctt
    22981 cagcgatcaa cggaacttct tcattgtcag atgatccagc tgaccaagaa gcgactatgg
    23041 caccaatctt tgacacgatt atcgaccata tcccagctcc agtagataac tcagatgagc
    23101 ctttgcagtt ccaagtgtca cttttggact acaatgactt cgttggacgt atcggtatcg
    23161 gtcgtgtctt ccgtggtaca gttaaggttg gggaccaagt taccctttct aaacttgacg
    23221 gtacaactaa aaacttccgt gttacaaaac tcttcggttt ctttggtttg gaacgtcgtg
    23281 aaatccaaga agccaaagcg ggtgacttga ttgccgtttc aggtatggaa gacatctttg
    23341 tcggtgaaac catcactccg acagatgcag tagaagctct tccaatccta cacatcgatg
    23401 agccaactct tcaaatgact ttcttggtca acaactcacc atttgctggt aaagaaggta
    23461 aatgggtaac ttctcgtaag gtggaagaac gcttgcaggc agaattgcaa acagacgttt
    23521 cccttcgtgt tgacccaact gattcaccag ataaatggac tgtttcagga cgtggagaat
    23581 tgcacttgtc aatccttatc gaaacaatgc gtcgtgaggg ctatgaactt caagtatctc
    23641 gtccagaagt tatcgtaaaa gaaatcgacg gtgttaaatg tgaaccattt gaacgtgtac
    23701 aaatcgacac tccagaagaa taccaagggt ctgttatcca aagcctttct gaacgtaaag
    23761 gtgaaatgtt ggatatgatt tcaactggta atggtcaaac tcgtttggtc ttccttgttc
    23821 cagcgcgtgg tttgattgga tactcaactg agttcttatc aatgactcgt ggttacggta
    23881 tcatgaacca taccttcgac caatacttgc cattgattcc aggggaaatt ggtggacgtc
    23941 accgtggtgc ccttgtttct atcgatgctg gtaaggctac aacttactca atcatgtcta
    24001 tcgaagaacg tggtacgatc tttgtcaacc caggtactga ggtttatgaa ggaatgatca
    24061 tcggtgaaaa ctctcgtgaa aatgacttga cagttaacat cacgaaggca aaacaaatga
    24121 ccaacgttcg ttcagctact aaggaccaaa cagctgttat caagacgcct cgtatcttga
    24181 cacttgaaga gtctcttgag ttcttgaacg acgatgagta catggaagta acgcctgagt
    24241 ctatccgttt gcgtaaacaa atccttaaca aagcagagcg tgagaaagct aacaagaaga
    24301 aaaaatcagc tgaataagag ctagaaagag agaaagatgg tctatttagt cctaggaatt
    24361 ttactgctcc tactctatgt atttgcgaca ccagaaagca ttaaagggac tgtcaatatc
    24421 gtcgctatgg tatgtatttt agtggcactc ttgattttat tggttctatc ttttctgaaa
    24481 atttttcaat taccaacaga aatattccta gcaatagcca tgttgatcct agcttacttt
    24541 agtgttagag acatcacact catgccagtc aaaaaaagta aaagaagata aaaaagagag
    24601 tttcggctct ctttttgcta ggaattttac aatagtttga attgcttttt agttaacacc
    24661 atctctttta cttgtatagt atgaatggga aaaacttgaa taaaattcgt taat
//
LOCUS       DAVMEF010000010        24600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMEF010000010 DAVMEF010000000
VERSION     DAVMEF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.010"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(20..1357)
                     /locus_tag="VYQ30_000279"
     CDS             complement(20..1357)
                     /locus_tag="VYQ30_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1965451.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1367..1633)
                     /locus_tag="VYQ30_000280"
     CDS             complement(1367..1633)
                     /locus_tag="VYQ30_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1965452.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1898..2284)
                     /gene="rplQ"
                     /locus_tag="VYQ30_000281"
     CDS             complement(1898..2284)
                     /gene="rplQ"
                     /locus_tag="VYQ30_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1965453.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2296..3231)
                     /locus_tag="VYQ30_000282"
     CDS             complement(2296..3231)
                     /locus_tag="VYQ30_000282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1965454.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3274..3657)
                     /gene="rpsK"
                     /locus_tag="VYQ30_000283"
     CDS             complement(3274..3657)
                     /gene="rpsK"
                     /locus_tag="VYQ30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1965455.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3675..4040)
                     /gene="rpsM"
                     /locus_tag="VYQ30_000284"
     CDS             complement(3675..4040)
                     /gene="rpsM"
                     /locus_tag="VYQ30_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1965456.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4058..4174)
                     /gene="rpmJ"
                     /locus_tag="VYQ30_000285"
     CDS             complement(4058..4174)
                     /gene="rpmJ"
                     /locus_tag="VYQ30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1965457.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4199..4417)
                     /gene="infA"
                     /locus_tag="VYQ30_000286"
     CDS             complement(4199..4417)
                     /gene="infA"
                     /locus_tag="VYQ30_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1965458.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4534..5172)
                     /locus_tag="VYQ30_000287"
     CDS             complement(4534..5172)
                     /locus_tag="VYQ30_000287"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1965459.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5323..6633)
                     /gene="secY"
                     /locus_tag="VYQ30_000288"
     CDS             complement(5323..6633)
                     /gene="secY"
                     /locus_tag="VYQ30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1965460.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6646..7086)
                     /gene="rplO"
                     /locus_tag="VYQ30_000289"
     CDS             complement(6646..7086)
                     /gene="rplO"
                     /locus_tag="VYQ30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1965461.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7231..7455)
                     /gene="rpmD"
                     /locus_tag="VYQ30_000290"
     CDS             complement(7231..7455)
                     /gene="rpmD"
                     /locus_tag="VYQ30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1965462.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7427..7921)
                     /gene="rpsE"
                     /locus_tag="VYQ30_000291"
     CDS             complement(7427..7921)
                     /gene="rpsE"
                     /locus_tag="VYQ30_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1965463.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7939..8295)
                     /gene="rplR"
                     /locus_tag="VYQ30_000292"
     CDS             complement(7939..8295)
                     /gene="rplR"
                     /locus_tag="VYQ30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1965464.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8379..8915)
                     /gene="rplF"
                     /locus_tag="VYQ30_000293"
     CDS             complement(8379..8915)
                     /gene="rplF"
                     /locus_tag="VYQ30_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1965465.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9107..9505)
                     /gene="rpsH"
                     /locus_tag="VYQ30_000294"
     CDS             complement(9107..9505)
                     /gene="rpsH"
                     /locus_tag="VYQ30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1965466.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9719..9988)
                     /gene="rpsN"
                     /locus_tag="VYQ30_000295"
     CDS             complement(9719..9988)
                     /gene="rpsN"
                     /locus_tag="VYQ30_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1965467.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10006..10548)
                     /gene="rplE"
                     /locus_tag="VYQ30_000296"
     CDS             complement(10006..10548)
                     /gene="rplE"
                     /locus_tag="VYQ30_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1965468.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10572..10877)
                     /gene="rplX"
                     /locus_tag="VYQ30_000297"
     CDS             complement(10572..10877)
                     /gene="rplX"
                     /locus_tag="VYQ30_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1965469.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(10955..11323)
                     /gene="rplN"
                     /locus_tag="VYQ30_000298"
     CDS             complement(10955..11323)
                     /gene="rplN"
                     /locus_tag="VYQ30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1965470.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11349..11609)
                     /gene="rpsQ"
                     /locus_tag="VYQ30_000299"
     CDS             complement(11349..11609)
                     /gene="rpsQ"
                     /locus_tag="VYQ30_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1965471.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11634..11840)
                     /locus_tag="VYQ30_000300"
     CDS             complement(11634..11840)
                     /locus_tag="VYQ30_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1965472.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11850..12263)
                     /gene="rplP"
                     /locus_tag="VYQ30_000301"
     CDS             complement(11850..12263)
                     /gene="rplP"
                     /locus_tag="VYQ30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1965473.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12267..12920)
                     /gene="rpsC"
                     /locus_tag="VYQ30_000302"
     CDS             complement(12267..12920)
                     /gene="rpsC"
                     /locus_tag="VYQ30_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1965474.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(12933..13277)
                     /gene="rplV"
                     /locus_tag="VYQ30_000303"
     CDS             complement(12933..13277)
                     /gene="rplV"
                     /locus_tag="VYQ30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1965475.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13289..13570)
                     /gene="rpsS"
                     /locus_tag="VYQ30_000304"
     CDS             complement(13289..13570)
                     /gene="rpsS"
                     /locus_tag="VYQ30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1965476.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13674..14507)
                     /gene="rplB"
                     /locus_tag="VYQ30_000305"
     CDS             complement(13674..14507)
                     /gene="rplB"
                     /locus_tag="VYQ30_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1965477.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14525..14821)
                     /locus_tag="VYQ30_000306"
     CDS             complement(14525..14821)
                     /locus_tag="VYQ30_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1965478.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14821..15444)
                     /gene="rplD"
                     /locus_tag="VYQ30_000307"
     CDS             complement(14821..15444)
                     /gene="rplD"
                     /locus_tag="VYQ30_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1965479.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15469..16095)
                     /gene="rplC"
                     /locus_tag="VYQ30_000308"
     CDS             complement(15469..16095)
                     /gene="rplC"
                     /locus_tag="VYQ30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1965480.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16311..16619)
                     /gene="rpsJ"
                     /locus_tag="VYQ30_000309"
     CDS             complement(16311..16619)
                     /gene="rpsJ"
                     /locus_tag="VYQ30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1965481.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16886..17503)
                     /locus_tag="VYQ30_000310"
     CDS             complement(16886..17503)
                     /locus_tag="VYQ30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1965482.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELENLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17500..18090)
                     /gene="nrdG"
                     /locus_tag="VYQ30_000311"
     CDS             complement(17500..18090)
                     /gene="nrdG"
                     /locus_tag="VYQ30_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1965483.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGILYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18083..18589)
                     /locus_tag="VYQ30_000312"
     CDS             complement(18083..18589)
                     /locus_tag="VYQ30_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965484.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(18633..18773)
                     /locus_tag="VYQ30_000313"
     CDS             complement(18633..18773)
                     /locus_tag="VYQ30_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965485.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18785..20992)
                     /gene="nrdD"
                     /locus_tag="VYQ30_000314"
     CDS             complement(18785..20992)
                     /gene="nrdD"
                     /locus_tag="VYQ30_000314"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1965486.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(21112..22650)
                     /locus_tag="VYQ30_000315"
     CDS             complement(21112..22650)
                     /locus_tag="VYQ30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1965487.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLDLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(22687..22830)
                     /locus_tag="VYQ30_000316"
     CDS             complement(22687..22830)
                     /locus_tag="VYQ30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965488.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(22904..24436)
                     /gene="cls"
                     /locus_tag="VYQ30_000317"
     CDS             complement(22904..24436)
                     /gene="cls"
                     /locus_tag="VYQ30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1965489.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
BASE COUNT         6920 a         5454 c         4600 g         7626 t
ORIGIN      
        1 taaaatttct cctattttct taatttttaa aactatgaat tggtgctggg atttgtccac
       61 cgcgagagat gaagtcgaca gacgaagccc cattaaccct cataacgggt gcagttccta
      121 atagaccacc aaactcaatc atatcgcctt cttttccttt gggaatgata cgaacagctg
      181 ttgttttcat gttgataaca ccgattgctg cttcatccgc aatcatagcc gcaatagttt
      241 cagcaggcgt atcttctggg atggcaatca tatccaatcc aacagaacag atagccgtca
      301 tagcttctag tttttctaaa ttaagagagc cattttgcac tgcagcaatc attccttcat
      361 cctcagaaac agggataaag gcaccagata gaccaccgac ctggttacag gccatcactc
      421 cacccttttt aacttggtcg ttcaagaggg ccaaggcagc cgtcgttcca tgcgtgccaa
      481 ctgtttctag ccccatttcc tcaaggacac gtgccacaga gtctccaacc gcaggggttg
      541 gtgccaaact caagtccaca ataccaaact ccacacccag tctctcactg gccatttgac
      601 caaccaattg accgatacga gtgattttaa aggcagtttt cttaactgtt tcggctacta
      661 catcaaagct ctgtccacga actttttcca aagcacgttt cacaacacca ggaccagaaa
      721 ctccgacatt gatgataaca tctgcttccc caacaccatg aaaggcaccc gccataaatg
      781 gattgtcctc aacagcatta gcgaatacaa ccaacttggc cactcccata tctgaaagat
      841 ttgctgtttc cttgataatt cgtcccatat ctgccacagc cgtcatatta ataccagact
      901 tggttgagcc gatattgact gacgagcaga ccttatccgt ctcagccaaa gcgcgaggaa
      961 tggaattgat gagaatctca tctccctttt gataaccttt ttgtactaag gcagaaaaac
     1021 caccaataaa gtccacacca atctctttcg cagccttatc aagcgctttt gccagaacca
     1081 cgtagtccgt cgcatctgtc gctgccccaa tcagagaaat aggtgtcacc gatacacgct
     1141 tattaacgat aggaattccc aactcagccg caatttcatc accaacagct actaaattag
     1201 ccgcctttgt cgtaattttt tgatagattt tctccgcagc acgattgata tctggatcga
     1261 tacagtccaa aagagaaatc cccatggtaa tggttctaat atcgaagttt tgctcctcaa
     1321 tcatggcgat ggtttcagta acttgtctaa tatccatgat gacctcctag atattataca
     1381 tagcttcgaa aatcgctgca ctctgaatat tgatttttac attcaaagtt tgcccaaaag
     1441 cttcaaattc attacgaaga taggtaaaat cttgcttttc atcactagat acaacagcca
     1501 tcatcgtaaa atattcatcc aatacagttt gagagatatc gtcaatattc aatcctaatt
     1561 ctgcaatttt accagaaaca cctgcaacaa ttccagattt atctttacca acaacagtta
     1621 taatggcttt cataagtaaa ctccaattct tcttaaaata agaaacctta acattaaaca
     1681 ggattctttt caaatagtat agcataattc taacgaaaat actcaaaaac gcctgcttac
     1741 gaagttgttc ttaagaaaaa aacaatttcg taaacaagcg tctactatcc caacttatga
     1801 tgagtgttcc cgctaggacc gaaatcctag cggtagacca gagctagact aagagcacaa
     1861 gactccatca tcataacact caacaaaatt gatgatttta tactaattcg atgatcgcca
     1921 ttggcgctgc atcaccacga cgtggttcag ttttaaggat acgagtgtat ccaccgttac
     1981 gttcagcata acgaggtgcg atttctgaga acaatttttg aagtgctgta gtagaagtgt
     2041 acttatcagt tgcttcatca tagttttcag atgcgatttc attacgtacg aaagcagctg
     2101 cttgacgacg tgcatgcaaa tcaccacgtt tacctagagt aatcattttt tcaacagttt
     2161 tacggatttc tttagcacga gcttcagttg tcacgattga ttcgttgatc aaaaggtcag
     2221 ttgtcaaatc gcgaagcatt gctttacgtt gtgagctagt gcgtcctagt ttacggtaag
     2281 ccatgtattc ctcctttatt tatcttttaa tccaagaccc aaatcaatga gtttgagttt
     2341 cacttcttcc aaactcttgc gtccaagatt tcgtactttc atcatctctg cttcagattt
     2401 ttctgtcaaa tcatgcacag tattgatacc ggcacgtttt aaacagttgt atgaacgcac
     2461 agacaagtcc agttcctcaa tcgtacgatc taaaatacgg tcgtcagatt cagtatcagc
     2521 ttctttcatc acttcagttg acttagcaat ctcagtaaga tttgtaaaca aatcaagatg
     2581 ttctgtcaaa atacgtgctg aaagccctaa agcatcttct ggaataattg ttccatttgt
     2641 caagatttca agggttaatt tgtcgaaacc atcattgcta cctacacgag caggttccac
     2701 ttgatagttg acttttgtaa ctggtgtata aatagaatct acagcaagtg ttccaactgg
     2761 tgcattatcc tttttatttt cgtcagcagg tacatatcca cgaccactgt taacagtcat
     2821 agtcgctttt agagaagaac cttcaccgat tgtaaagaga taatgatctg gatttacaat
     2881 ttcaatatcg ctatctgtca aaatgtcacc agctgttact tcagcaggac cttcaacatc
     2941 cagttcgatg attttttcgt cttcaacgta cgatttcact gcaattcctt taatgttcag
     3001 aatgatttgc atcacgtctt cacgaacacc tggaactgtg tcaaactcat gtaacacacc
     3061 atcaatgttg atagatgtca cagctgctcc tggtagagaa gctagaagta cacgacgaag
     3121 agagttacca agagttgtac cgtagccacg ttcaagtggt tcgattacaa acttgccata
     3181 atctttattt tcatcaattt ttgttatatt tggtttttca aactcgatca tttagttact
     3241 ccctcttaaa cgaaaagcag tgtaatgcga tgattataca cggcgacgtt ttggaggacg
     3301 agcaccattg tgtggcactg gagtcacatc acgaattgct gttacttcaa gaccagcggc
     3361 agcaagcgca cgaatagctg actcacgacc agaacctgga ccttttacag taacttcaac
     3421 tgatttaaga ccgtgttctt gtgcagattt agcagcagct tcagaagcca tttgagcagc
     3481 gaatggtgta gatttacgag aacctttgaa accaagagca ccagctgatg accaagcaat
     3541 tgcattacca tgcacatcag taatcataac aatagtgtta ttaaatgtag cgtgaatatg
     3601 agcaatacca gattcgatat tctttttcac acgacgttta cgtgttggtt tagccaagac
     3661 ttttacctcc tatattattt tttcttacca gcaatcgcaa cagctttacc tttacgagtg
     3721 cgagcgttgt ttttagtatt ttgtccacgg acaggaagtc cacgacggtg acggatacca
     3781 cggtatgaac cgatttccat caaacgtttg atgttcaagt ttacttcacg acgaaggtca
     3841 ccttcaactt tgattgcatc cacttcacga cggatagcat cttcttgatc tgatgtaaga
     3901 tcacgtacac gaacatcttc tgagattcca gcagcagcca aaattttctt agatgttgca
     3961 agtccgatac cataaacata agtcaatgag attactacgc gtttgtcatt tggaatatca
     4021 actccagcaa tacgagccat gttttctcct ttctatctta tccttgacgt tgtttgtgtt
     4081 ttggatttgc tgggcaaatt accataacac gaccattacg acgaataact ttacagtatt
     4141 cgcaaattgg tttgaccgat ggtcttactt tcatttctta tccctccaag tttttcgatt
     4201 atttaaagcg gtaagtgata cgtccacgtg tcaagtcata tggactcatt tcgacagtaa
     4261 cacgatctcc cgctaaaata cgaatatagt ttttacgaat tttaccagaa actgttgcta
     4321 aaatctgatg tccattttca agttcaaccg taaacattgc attcggcatt gtatcaacta
     4381 ctttgccttc aacttcaatc acatcgtctt ttgccacgca aaagcacctc cataaatttc
     4441 gattcgatgc ctctagacac agagacaaca attataagtc agactatttc agtataacat
     4501 ttgtagcgga tttttgcaag tgtgaaaaac gctttatttc aaatttgtca atactttttc
     4561 aatatctgag aagacatcat tgatatcttg attaccttcg atgtcatgaa ccaaaccttt
     4621 ggcacggtag tgagcaatga ttggttctcc ttgagcaata ttaacatcca aacgacgttt
     4681 tactgtctca ggcttatcat cttcacgttg gtagtaatct tcttctttat agtcaactgg
     4741 tgggttaaag accttgtgga aagtttctcc agttacgcgg tggatgatac ggccactcaa
     4801 acgttccaag aggctgtcag ggttcacttc aatattgata ataccttcta gttcaatgcc
     4861 aagttcagcc aatgttttgt ccaaggcatg agcttgttca attgtacgtg ggtaaccatc
     4921 caataagaat cctgtttctt taatatcatc ttgtgaaagg cgttctttta cgattccatt
     4981 tgtaacttcg tcaggaacca attcaccctt gtcaatatat gacttagcaa gaacacccat
     5041 ttcagtttga tttgccattg cagcgcggaa catatcacct gttgagatat gtgcaacatg
     5101 gaattgttct acgatttttg ctgcttgagt tcccttacct gcaccaggta agcccataat
     5161 caaaagattc atgatttgat ctccttattt tatttttaaa tagaagcaaa aagttttttc
     5221 accccgattt aaaaacaaaa tagaagaggg gagatccaac tcttacaaat gtcagagttt
     5281 aaacctccac tccctcagta tttactgatt cagtaaatac ttttattctg ttctgtccat
     5341 gaaaccaaca tacttacgtt tcaataggta accttccaat tgcttgattc cttcgatacc
     5401 tgtagagata atgatcaaga gacttgttcc accaaaggca acaacatcag aaagaccaaa
     5461 tacatcttta gctgcaatcg gtaaaatgga aatcacacca aggaagaggg aaccaacagt
     5521 tgcaagacga cgaagaagtt tagacatata ttcttctgta cctttaccag gacgaactcc
     5581 atggatatag gcaccactct tttgtaggct ctctgctgct ttttcaggat taatctgtac
     5641 aaacgtatag aagaatgtaa agagaataat caacaaagca tacatggcaa taccagttgg
     5701 agaagtagtt gccaacatct cttgtgctac ccttacccaa gcccaatcat gacctgtggc
     5761 actcaaaaac tgaagaatag ccgcaggcgc tgcagtaatc gaactggcaa agataacagg
     5821 gataactcca gcaggattta cctttaacgg aaggtaagag ctagatggag caccttgtgc
     5881 aaccttagta tattggattg gaattttgta ttctgcttgt tgaacataag ttgtaaagta
     5941 aataatcaac aatacagtaa taatcaaaat gattacgaaa atgatagatg aagtgatacg
     6001 gctacttggg acgttcacaa agtagtccac atagatgccc tgaatcatct ctggaattga
     6061 ggaaacaatc ccggcaaaga taatcatgga aacaccgttt ccgtatccct tatctgtaat
     6121 ttgctcaccc aaccaagtga caatcatact accagctgtt aagatgatac caatcgtcag
     6181 aaaaacttgt ggagttaaag cagttttaat caattgagct ccagccaagg tattaaaacc
     6241 agctgtaatc ccgatagatt gcacaaaagc gagaactaga gcaatataac gagtagcttg
     6301 attcaatttt cttcgaccta cttccccttg tttaccccac tctacaaact tgggtaaaat
     6361 atccatttgc aagagttgga caacaataga agcggtgata taaggactaa ctcctagggc
     6421 aaaaatcgaa aagtttttta gggcattccc cgacaccaag ctcaacatgt ttaagaagga
     6481 taatccactt aaagcattca agctattggc attcacacca ggaactgtaa tgctagttcc
     6541 gatacgaaag accaaaacga taaaaattgt aaataaaatt tttgatcgaa cctgcttgac
     6601 tttaagagct tctcttaata atttaaaaaa cataggtcac ctctcttaga tgacttctac
     6661 tgaaccacct ttagcagtga tagcttcttc agctgattta gagaatttag ctgctttcac
     6721 agtcaatttc ttagtcaact caccgttacc aagaatttta atacctgact tttcagcttt
     6781 aacaattcct gcttcgataa gaacaactgg agttacttca gcaccatctt caaagacgtt
     6841 caattggtca aggttcacaa ttgcgtattc tttagcgttg atgttagtga atccacgttt
     6901 tggaagacga cggaacaatg gagtttgtcc accttcaaaa ccaaggcgaa ctccgccacc
     6961 gctacgagct ttttgacctt tttgaccacg accagatgtt ttaccgttac ctgatgaagt
     7021 accacgacca acgcggttac gtactttacg agaaccttct gcaggtttca attcatgaag
     7081 tttcattttt attttctcct cttttgtaaa atgctagcgc cgataaggga gaaaaggttg
     7141 tctcccccat caactcgcct atacgatgtc atcatagatg actatatcta gttttagggg
     7201 atggtacagt gcacatcccc taaaacttca ttagtttact tcttcaactg ttactaagtg
     7261 agatactgct gtaatcatac cacggatagc agcgttatct tctttaataa cagagctgtt
     7321 caatttgcca agtccaagtg ctacaacagt tttacgttgt gatggaatgc gtccgattgg
     7381 agacttagtc aaagtaattt taatttgagc cattttatcc cctttcttat gccaaatcag
     7441 aaactgaaat accacgaagg gcagcaattt cttcagcgcg tttcaattgt ttcaaacctt
     7501 caacagttgc acgaacaatg ttgattggag tgttagaacc aagtgattta gatgtaatat
     7561 ctgccacacc tgccaattcc acaacggcac gaactgcacc accagcggca actccagaac
     7621 cttctacagc aggtttcaac aatactttag ctccaccgaa ttctgaaaga acttcgtgtg
     7681 ggattgttgt tccaaccata ggaacttcga tcaagttttt cttagcatca tctactgctt
     7741 tacggattgc ttctggaact tcttgagctt taccagtacc aaatcctacg cgaccattgt
     7801 ggtcaccaac aacaacaaga gctgcgaaac gaagacgacg tccaccttta acaacttttg
     7861 taacacggtt gacagcaact acgcgttctt ctaattcaac tgcattgtct ttaaatgcca
     7921 ttttctagtg tcctcctatt agaatttcaa tccgttttca cgagctgcat cagccaaagc
     7981 tttcacacgt ccgtgatata gatatccacc gcggtcgaac accacttctg aaataccttt
     8041 agcgtttgca cgttctgcaa cgagtttacc gacagcaacg gcttgttcag ttttagttcc
     8101 ttttgaaact tctttatcaa gagttgaagc acttgcgagc gttacacccg ctacgtcatc
     8161 aatcacttga gcgtagatgc ctgtattaga acggaatacg ttcaaacgtg ggcgatcagc
     8221 agttccagag agttttccgc gaacgcgacg gtggcgtttt tggcggagtt tgtttttatc
     8281 tggttttgaa atcacagttt tcacctcttt agttttaaat cgtgtgctat gcacaaagtt
     8341 ggaaaatagg ttggtggttg atgatcaacc actcaacatt atttacctgt tttaccttct
     8401 ttacggcgaa cgaattcacc aacgtaacgg atacctttac ctttatatgg ttctggtgaa
     8461 cgaaggctac gtacgtaagc agctgtttga ccaactactt cttttgaaat tccgctaaca
     8521 acgattgttg ttgggtttgg aagttcaaaa gtaattcctt ctggagcttc aacttcgtct
     8581 ggatgagatt taccaacagc caaaacaagt ttagatcctt gaagctgtgc acggtaacca
     8641 accccacgca tttcaagttc tttcttgaat ccttctgata caccaacaac catgttgttc
     8701 aaaagggcac gagtagttcc gtggatagtt ttcatttctt ttgaatcgtt tggacggtga
     8761 agagttattt cagtaccttc cacacggatt tcaatatctt ttgagaactc acgagtaagt
     8821 tctcctttag gtccttttac agttacaacg ttgtcattgt tagcgagttc aacaccagca
     8881 ggcaacacga taactttatt accaatacgt gacatgttta ttttctcctg ttaaattgtc
     8941 aggccagaac ggccagtttt cacggggttc agatacttat ttagttcaag agctgaactg
     9001 aacacgctct aagaactgtg caaaatagat aagttggctt gtttgtaaac aaacttctcc
     9061 aacttcctaa ttttgctttg ttctttacga gctttgtatc ttgattttac caaacgtaag
     9121 cgataacctc accaccaaca ttcttttggc gtgcttcttt atcagtaagc aaaccttcag
     9181 aagttgaaag gatggcaatt ccaagtccgt taagaacttt tggaaggtct tcacgttttt
     9241 tgtagacacg aagtcctggt ttagaaacac gtttcaagtt agtgataact ttctcaccat
     9301 ttggtccgta tttaagaaat acacggatga cgccttgttt gtcatcttca atgatttcaa
     9361 cgttttttac aaaaccttcg cgtttaagga tttcagcaat cccttttttg atgtttgatg
     9421 caggtacttc aagtacttcg tgtttagctt ggttagcatt acgaatacga gttaggaagt
     9481 ctgcgattgg gtcagtcata accattttgt ttttctcctc ttactagtag tttgcaagtt
     9541 gcacttgcta gttaatgatt gagctgggct cagatagtat tgacacattc aaacgagaga
     9601 actcttattt gaacacgagc tacaacctgt gcaaaaaaga taaatttgtt ttggagcatc
     9661 gctccctcac caaatttcct attttcgctg tggttgttac gctctttgta tcatgatatt
     9721 accaagatgc ttttgttaca ccaggaattt gacctttatg cgcaagttcg cgaaaagcga
     9781 tacgactcag accaaatttg cggtaaactg aatgtgggcg ccccgtaact ctacaacgat
     9841 tatgtaaacg agtcggtgag gcgttgcgag gtaatttaga taaaccttcg tagtcccctg
     9901 ccgcctttaa tgcagcacgt ttttcagcat aacggtcaac aattttttgg cgtttagcct
     9961 ctctagctac cattgatttt ttagccatta gatttacctc ctatattatt ttgcaaaagg
    10021 cattccaagg cctgtaagca atgcacgtga ctcttcgtca gtgttagcag ttgttacgat
    10081 aacgatgtca agaccacgag ttttgtcaac gtcatcgaag ttgatttctg ggaagattaa
    10141 ttgttctttc acaccaagtg tgtagttccc gcgtccatca aatgattttg ttggaacacc
    10201 gtggaagtca cgtacacgcg gaagtgaaac tgataccaat ttatccaaga attcgtacat
    10261 acgttcacca cgaagggtaa cttttgcacc gatcgcaaca ccttcacgaa gacggaagcc
    10321 ggcgattgat tttttagctt tagtgataag tggtttttga cctgagataa gtgccaattc
    10381 ttcagcagct ttttcaaggc ttttagcgtt tgatacagct tcaccaacac ccatgttcaa
    10441 aacaatctta tctactttag gcacagccat cactgatgag tagttgaatt gttctgtcaa
    10501 agcaggaact acttcattaa gatatttttc ttttaaacga tttgccatta tacttctcct
    10561 ttccttcgtg attaatcaag cacttcgcct gattttttgt tgtagcgaac ttttttaccg
    10621 tctacaaatt tgtatccaac acgaccagct acaccatttt tgtccaaaac ttgaacgttt
    10681 gatacgtgga tagctgcttc tttctcgatg ataccacctt gaggaagctc gttagttgga
    10741 cgttggtgtt tcttaacaat gttaacacct tcaacgataa ctttgtttac ttttggaagg
    10801 gcagtaagga caacagcttc tgttccctta tctttaccag cgattacgcg aactttgtcg
    10861 ccttttttta caaacattag gtttctcctt gatttttctt acgcccataa gggcacccta
    10921 gcttgaagct aggggactag tttgttccta aaaattaaag tacttctgga gcaagtgaca
    10981 cgatcttcat gaagccacct tcacgcaatt cacgtgcaac tgggccaaag atacgtgttc
    11041 cgcgaggagt tttgtcttca cggatgataa ctgctgcgtt ttcgtcaaat ttgatgtatg
    11101 aaccatcagc acgacgagca cctgatttag tacgaacgat aactgcttta acaacgtcac
    11161 cttttttaac cgcaccacca ggagtagctt gttttacaga tgccacgata acatcaccga
    11221 tgtttgcaaa tttacgtcct gaaccaccaa gaactttgat agtcaagatt tcgcgagcac
    11281 cgctgttgtc tgcgactttc aaacgagttt ctgtttgaat catttcagtt ttctcctttc
    11341 aggtttgatt agatgatgac cgcttcttca acaacttcta caagacggaa acgttttgta
    11401 gctgaaagcg ggcgagtttc catgatacgt acgatatcgc cttctttggc aacattgttt
    11461 tcatcatgag ctttgtattt tttagagtag ttaatacgtt taccatagac tgggtggtta
    11521 cgttttgttt caactacaac tgtgattgtc ttgtccattt tgtcagatac aacacgtcca
    11581 acaagaactt tacgattatt gcgttccatt gaaatttctc cttccctagt ctattatttc
    11641 gcttcagatt gaactgtttt gatacgagcg atttgttttt taacttcttt caagcgagct
    11701 gtttgttcca attgaccagt agcagcttgg aaacgaagtt caaacaattc ttttttcaat
    11761 tcgttttcgc gcttcgcgag ttcttcttga gaaagaccac gaagttcttt aacaaattct
    11821 tttacttcat taagtttcat gccttctcct tattctgctt cacgttttac gaatttacat
    11881 ttaactggca atttgtggct agcaagtcga agcgcttcac gtgcaatctc ttcagataca
    11941 ccagcgattt cgaacatcac tttaccacgt ttaactggtg ctacccaacc ttcaggtgcc
    12001 cctttaccag atcccatacg cacaccgata gctttagcag tgtatgattt gtgtgggaag
    12061 attttaatcc aaactttacc accacgtttc atgtaacgag tcatggcgat acgagcagct
    12121 tcgatttggc ggttagtgat ccagtggcta gttgtagctt gaagaccgta ttcaccgaat
    12181 gctacttctt ttccaccttt tgcttcaccg cgcatttttc cacggaattc acgacggtgt
    12241 ttaacacgtt taggtactaa cattggttat ttacctcctt tagtgttttt acgagctgga
    12301 agaacttcac cacggtagat ccatacttta acaccaagtt taccgtatgt agtatctgct
    12361 tcttcccaag cgtaatcgat atctgcacga agtgtgtgaa gcggaacagt tccttcagag
    12421 tatccttcag cacgggcgat atctgcaccg ttcaaacgac ctgatacttg agttttgatt
    12481 cctttagctc cagcacgcat tgcacgttgg attgcttgtt tttgtgcacg acggaaagca
    12541 acacgttgct ccaattgacg agcaattcct tcacctacaa ggtgagcatc caaatcaggt
    12601 tgtttgattt cgatgatgtt gatgtgtact tgttttccag tcaatttgtt aagttttgca
    12661 cggagtgcat caacgttagc accaccttta ccgataacca tacctggttt agcagtgtga
    12721 agtgaaacgt taactttgtt tactgcgcgt tcgatttcaa tagttgaaac tgctgcgtca
    12781 gcaagttctt tttgaacgaa tttacggatt gcaagatctt catgaaggta atccgcgtat
    12841 tctttttcag cataccattt ggcatcccaa tcacggatga tgccgacacg cataccaatt
    12901 ggatgtactt tttgacccac gattttacct ccttattttt ctgcaacagc tacagtgatg
    12961 tgagctgtac gtttgttgat tggtgaagct gaacctttcg cacgtggacg gaaacgtttc
    13021 atagttggtc cttcgtttgc gaatgcttca gatactacca agttagcttt atccaaacca
    13081 aagttgtttt cagcgttagc tacagctgag ttcaaaactt tcaagatgat ttcagcagct
    13141 ttgtttggag tgaatgtcaa gattgcgatt gcatcggcta cgcttttacc acggatgtta
    13201 tcaagaacaa gacgtgattt acgaggtgaa acacgtactg tacgagccat tgctttagct
    13261 gaagtaattt ctgccattta tgttctcctt attttctacg tgttttcttg tcgtctgcag
    13321 cgtgaccttt gtaagtacga gttggtgcaa attcaccaag tttgtggcct accatgtctt
    13381 cttggatgta aacaggtacg tgtttacgtc cgtcataaac tgcaatagtg taaccaatga
    13441 aacttgggaa gatcgttgat cgacgtgacc aagttttaat aacttttttc ttttcgtcgt
    13501 tagcttgagc ttcaactttt ttcatcaaat gctcatcgac gaaaggtcct tttttaagac
    13561 tgcgtcccat ttttatattt tctcctttaa atgttgtacc acagcggctt gcactcctat
    13621 ggagcgctac cgagctggcg gatttactag ttgcttaagc gactagttta atattatttc
    13681 tcgttgcgac gacgaacgat aagtttgtca gatttcgctt tcttgttacg agttttaaga
    13741 ccaagagcag gtttgcccca tggagtagat ggtgctttac gaccaactgg tgccttacct
    13801 tcaccaccac cgtgtgggtg atcgttaggg ttcattacag aaccacgaac tgttgggcgg
    13861 atacctttcc aacggctacg tcctgcttta ccaaggttta caagtccatg ttgttcgttt
    13921 ccgacaacac caactgtagc acggcaagtt ccaagaatca tacgaacttc acctgattga
    13981 agacgaacaa gaacgtattt accttcagaa cccaatactt gagcagatgc accagcagca
    14041 cgtaccaatt caccaccacg acctggtttc aactcgatgt tgtgaatcaa agtaccaact
    14101 gggatgttag caagtggaag agcgtttccg actttgatat ctgcttctgg acctgaaacg
    14161 atacgttgac ctacttcaag accttttgga gcgatgatgt atgctttcac accatcagtg
    14221 tagtgtacaa gagcgatgtt tgcagaacgg tttggatcgt actcgattgt tttaacaact
    14281 gcttcaacgt tgtctttatt acgtttgaag tcaaccaaac ggtagaaacg tttgtgtcca
    14341 ccaccttggt gacgaactgt gatacgaccg ttgttgttac gaccagcctt gctcttcaat
    14401 gcaacaagca atgatttttc aggagtgctt gttgtgattt cagcgaaatc caaagaagtc
    14461 atattacggc gaccgtttgt tgttggttta taaacacgaa ttcccacgat atttcctcct
    14521 tagattattc agcttcagca gcaaacaact cgattgcttt agaatcagct gtaagtgtga
    14581 tgatagcttt tttagttttg ttagtaaaac cagtgtaacg tccaacacgt ttagcttttg
    14641 gttttacgtt gattgtgtta acattggcaa ctttaacacc ttcgaaagca gcttcaacag
    14701 cttgcttgat caaaagtttg tgtgcacgag tgtcaacttc aaatacatat tttcctgctt
    14761 caagttgagc cattgagctt tcagtgatga caggtttttt gataacatca tacaaattca
    14821 ttatgcaaga acctcctcga ttttagagat agctgcttgt gtgacaagaa gtttgtcgct
    14881 atttgcgatg tcaagaacac ttgcagttgt agcagttgca actttcacgt ttggaaggtt
    14941 acgagctgaa agagctgcga attcatttcc ttcttcaagg ataacaagaa ctttagaatc
    15001 gatgctcaat gctgcaagaa cttttgcaaa ttcagcagtt tttggagctg taaatgaaag
    15061 agcgtctaca gctacgaatt tgttttcagc aactttttca gagtaaactg atttaagagc
    15121 taggcgacga actttttgtg gaagtttgta gccgtatgaa cgtggagttg gtccgaagac
    15181 aacaccacca ccacgccatt gtggtgagcg gatagaacct tgacgagcac gtccagttcc
    15241 tttttgacgc catggtttgc gtccaccacc tgatactgca gagcggtttt taacagcgtg
    15301 tgttccttga cgaaggcttg cgcgttggct gatgattaca tcaaacacaa ctgattcatt
    15361 tggttcgata ccaaatactg catcgctaag aacaacttgg ccagcttctt taccagtttg
    15421 gtcaaataat gttacgtttg ccattgtgac tgatttcccc tttctttatt atttaccagc
    15481 tttaactgct gatttgatag tgataagaga tttcttagca cctggtacgt tacctttgat
    15541 aaggataacg ttcttttctg gaacaacttg tacaacttca aggttttgaa ttgttacgcg
    15601 gtcgccaccc atacgtcctg caaggttttt acctttgaat acgcggttag gtgcaacagg
    15661 ccccatagaa cctggacgac ggtggtaacg agaaccgtga gccattggtc cacgtgattg
    15721 tccgtggcgt ttgataacac cttggaaacc tttaccttta gaagtacccg ttacgtcaac
    15781 aacgtctcca gctgcgaatg tttcaactgt aatttcagca ccaacttcca agccttcaac
    15841 gtttttgaat tcacgaatga agcgcttagg agccgtgtta gctttcgcta catgtccttt
    15901 agcaggtttg ttgctcaata cttcgcgttt gtcatcgaaa ccaacttgga tagcgttgta
    15961 tccgtctgtt tcaacagttt taacttgaag aacaacgttt ggagttgctt caataactgt
    16021 tacagggatc aattcgccag cttcagtgaa gatttgagtc attcccactt ttttccctaa
    16081 gattcctttt gtcatgagaa aatagttcct tttctatatt tttattcaaa aagtttttaa
    16141 cgagcgtttt ttatgctcaa gtctaagata caaagggcgt caaactcaac gtgaaaatag
    16201 gaaactgaca cagtgtctag cagacgcgag gaagtttatc tttttcacac cgagtttagc
    16261 ccgtgttcaa ttagattgaa acaccagcct ctgctctttt atattttaga ttaaagtttg
    16321 atttctacgt ttacaccact tggaagatcc aatttcatca aggcatcaac tgttttttga
    16381 gttgggttaa cgatatcgat caaacgtttg tgtgtacgca tttcaaattg ttcgcgagag
    16441 tctttgtatt tgtgagtcgc acgaatgatt gtgtagaggc tacgttcagt tggaagtggg
    16501 attggacccg caacttgtgc acctgtacga gtagctgatt ctacgatttt tgcagccgct
    16561 gtgtcaagcg tacggtgttc gtaagctttc aaacggatac ggattttttt gtttgccatc
    16621 tttttctcct tttcgtctat ttaagataat aggctagctc cacaagaaaa ccgacgcggt
    16681 gttgcgtggc aatgcaaccg agcgtgtcgc aacctcttgc atcaaaacta aggctgtaat
    16741 ttacagcacc ataatagaat aacacaaagc ccctgcgatt gcaagggatt tgttgctgtt
    16801 ttttcgtttt tttgggatga aactgttttc ctactttttc tttctaaaat tagggatttt
    16861 cttctatatt tcttctatac ataagttaag tcctcttgac atcaaattta tcttctgatt
    16921 ggtccactaa gatatccgcc ttagactcag tttctttata gtatcgcaga tactgctccc
    16981 gtctcatctg atggctagcc aatataaagg atgcatcgcg atttctcaca gtcgtatctc
    17041 tagcaaggcg tcgctttaat tcggtctcct catccgtgta gaaacagatg gttttttcaa
    17101 agagttcctt gggtagaaag ccaacagaca tcccttcgac aatcaaaatt ggtttggctc
    17161 cagacaagac ctcactagcc ttccaaggtt cttcaattgt caagacatcc atacccgcct
    17221 gcaaggcaag gatatctctc tgcaaattct ccagttcatg cgccactggc agactggctg
    17281 tcaccttttg attcggcgcg tccttgggta ctaccaaata gcgtccggag gtgatatagg
    17341 gatctgtttc tagcaaattt actgtagtag aatctaaagc ttggtacaat tcctgtgcaa
    17401 aggttgattt acctgaagct ccatgaccgt atattcccag tgtcctgact ttccccgtct
    17461 cgatctctga gactagttgg tctactaagt cttttttctt cattctctct tcacctgttc
    17521 atagctttct tttccgtcat tgagcttgtc ccaaatcact acttgcccac ttttgagcga
    17581 tttttgcaca tcgataattc gttggttgga cgaacctcga aactggagca taagatttct
    17641 cttagttcga tcatatcttc catcgacaag aatgtcaatc agtgacaaga gttccagttt
    17701 atctggagtt tccaacatca tttcttccca agtgtagccc gtccaggacc aaatgtcctt
    17761 gtctggcaat tcctttcgga tgcgtttaac tagaggcaag agaatgccag tattgagaaa
    17821 aggctcccct cccagcaaag tcaagccttg aacatagggt tgggcaaggt ctgccatgat
    17881 ctgctcttct aattctgctg tatagagaat gccagcatta aaagaccaag tcgcaacatt
    17941 ataacatccc tcgcagtgaa acatacagcc tgatacatag agagagttac gcacgccttc
    18001 gccatcaaca aagttaaagg ccttgtagtc aatgatacga ccttgactaa gttcctcgct
    18061 tttccattct tgtggttttg gattattcat tcgctacctc tatccaataa cgctcgactc
    18121 cattgcgagc atcctcaaat attccaccat ttgctagaat gactgctctg ctagcaggat
    18181 tattcacact acaggtcacc agagctttct tgatgttctt ttccttagca acttgcaagc
    18241 cctgacggag agtctctttt gcataaccct tgcctctttc agatggacga atggagtagc
    18301 caatgtggcc accttcttct agtagaaagt tactgaggcg caaccggaga ttaagaaatc
    18361 caactgcttg acctttctca gaaaaagcca ctaactgaat tgcaggaacc catccttcag
    18421 gcagattaat ccccatttcc tgttcctgat tgctttctaa ccagtcttca tacacaaagt
    18481 tctctgtatc ccagaaaccg ccgtcgtgag gcgactgaaa tttttcaaac tctgtcatca
    18541 tatctaaaac agctttctta tccgctaatc ttggtctgcg taattccatc cttacctccc
    18601 actatgagaa aagcgagagc tggctctcac ttttattttt tcttgttctt gtttaaaaat
    18661 tgttctctga agctaagcaa gcgttctttc ttaccagctc cacctttaga gtgtttctcg
    18721 tgataacggg tcacttgtgc gcgcccctta tcgtctagtt gatattttcc catttcattt
    18781 ctttctaatt tgttacttga tgcccagcta ttttaatcgt tgaaccattc atatgtttga
    18841 cacgcgcagc gatttccttg tgacggccgt tgaccatcgg tctcgcttga ggattgccaa
    18901 gatagccaca agtccgttta acaacgtcta ctgttttagg atcgctattg ccacagtttg
    18961 gacaagcaaa ccctctctca gttggttcaa aatccccttc aaagtcacac ttgtagcaac
    19021 ggtcaatcgg agtattggtg cctagatagc ctacacggtc ataagcataa tcccagacag
    19081 cttccaaggc ctttggattt tgctgaagga ctggatactc acaataatgg atgaaaccac
    19141 ctgacgcacc tgcttctgga tagactttct caaagtccaa tttttcaaac ggtgttggat
    19201 ttttacgaac atcgtagtgg aaagagttgg tgtagtattc cttgtctgtg atatcaggaa
    19261 tagagccaaa cttgtctata tctagtcggc agaaacggtc tgtcagactt tcggatggtg
    19321 ttgagtagat agagaaatgg tagccatatt ggtctgacca ctcttctaca cggcgtttca
    19381 tatcgtgaat gatgtctagc gtgaattcct tagcatctgg attactttcc cagctgttac
    19441 caaagaaaac tgtcgctact tcatacaagc cgatatagcc cagcgaaacg gtcgcacgac
    19501 gattcttaaa gagctggtca acactttctt ctttacctag acgatggcca aaagcaccgt
    19561 actgataaag gataggagca ttcgctggtg tcgcctcttt agtgcgttcg acacggtaaa
    19621 caagagcatc ttctgcgata ttcattcgct cgttgaagat ttcccagaac ttattcatat
    19681 caccttcaga ctcaagagca atacgaggca gattaaccgt cacaacaccc agattcatgc
    19741 gacctgaatt gacttctaca ccattttcat ccttccatcc ttggaggaaa gaacggcaac
    19801 ccataggcac cttgaaggat cctgtcaagt caacaatctt atcgtaagat aaaacatctg
    19861 gatacatccg tttggttgca cactcaagag ccaactgctt gatgtcgtag ttgggagttc
    19921 cttcctctaa attaaggcct cttttcagag tgaaaataag tttaggaaag atagccgtac
    19981 ggtgttctga accaagaccc ttgatgcgaa tgtttaaaat agctttttga atttctcgtt
    20041 caaaacgact ggttcccaga ccaaaaccta gcgaagtaaa aggtgtttgt ccatttgaag
    20101 taaagagagt attgatttca tactcaagag attgcatggc atcgtagatg tccttttgcg
    20161 ttttcttcca agcgtaatct tcccgttttt caggcaatac ccactcttct gcatctttga
    20221 gatgtttttg atagttcttc tctgcataag gcgccaaaat ttcatcgata cggtcagctg
    20281 aacagccacc gtactggcta gaagcaacgt tggcaatgat ttgagaaatc tgtgctgtcg
    20341 cagtctggat agacttggga ctctctacct ctgcatttcc aatcttaaaa ccattttcca
    20401 acataccctt aaaatcaatc aaacagcagt tggtcatagg ggtataggga ctgtagtcca
    20461 aatcgtgata gtggatatcc cccttttggt gggcattggc tacgtgctta ggaagcattt
    20521 gcagtccgat tgatttccca acaatccctg ctgtcaaatc acgctgagtg ttaaagacat
    20581 cactgtcttt attagcattt tcattgacaa ctgtctggtc tttgttgaga agtttatgaa
    20641 tactaaagtt gatatccgtc gcttttgagc gctcaaaatc cctctgtgtc cgataagtaa
    20701 tatactcctc agccagcgca tattctttgg cttcaaggag ttcatgttct acgatatttt
    20761 gaatttcgta aatcttaatt ccctgtggaa agcgactatg aatttctgta ataattcgct
    20821 cagtcagatc attgaggcgt ttttcaacca ggggtgtcac atccataacc ttgtcagccg
    20881 ccttgtggag agccttgtca atcttgtcca catcaaatac aacacgtctc ccatctcgtt
    20941 tctcgacgaa gagagttggc aaaattgtaa ttttttcttc tagtgcaatc atacgcatgc
    21001 tcttttctaa aatgttcttt ctaaaaagta ttataccact ctaaaaagaa aaatcaatat
    21061 cttgtgttaa aaatcctaaa atttttaaaa atacacaaga tattgatttg gttatttaat
    21121 tttatagact ttaaactctg agtaatcagt ctttacttgt tggtaatttt cttttaagag
    21181 tgtttccact tcagaccaga ctaccacctt gtcatttacc acaatcacct taggttgttt
    21241 ttctttcaag tcattaagta acttattacg attttcctca gttgctgtat agtgcaacgg
    21301 ggacaaaatc gccgatggcg acaagcgctc actcttacga taaagagtcg cagtatcatc
    21361 ccatgcatag atagcatctt ccgtatttgt ctcctctttt accaaatcag caagacgatt
    21421 acgttcttga taaggtactg gataaaggac aaacttcatc aaaaaaggaa cagacaaaag
    21481 atagacaaac ccaaaaacta gcagatatag atttcctttg aagaaacgac tccataggct
    21541 aactggcgtt tctcttcggc ttcttctagc actacgcccc ctctgttgac tcttaatatt
    21601 ggttaccaat aaaagtagga aaacagggaa gacgaccatc aaataagaag cgtgcagagg
    21661 ctcttgtgaa aagaaaagta aaatcaaacc taaaattacc acaagacttg caggaaccga
    21721 aatgacgtat agtttaaagg gtttggattg aaagagacct aaaaagatac aaaccacaaa
    21781 tcccaaacca agggacaaca agccataaaa aaacatattc tctaaaattt tagaagtaaa
    21841 atcaaagcga atactgtcaa taggataacg aataccacta atcgcatccc caaaacttcc
    21901 tgttgcagca ctatagtagg cagttggata aaagacaagt gaaaaaccta aagccactgc
    21961 aagaaactga taaaacccat gcgcaaagcg tctatgccca aggttaaaga ccaacaagtc
    22021 taaactcact acagcaataa acaggagcga tgataaggga gcaaagaaaa atccgcctgc
    22081 caaagctagc ccaatccgta caaatccctt atcatggctt ggattgctta ggtaagccgc
    22141 aactaaacta aaggctgcga ataagaaagg aagcgctaaa agagtcgcat agcctccacc
    22201 aaaagcaaga cctgtaacta gcatgtaaaa aatagtcacc agatgtccag cttggtctcc
    22261 ttgctctgtc aaggtgtccg ctgatctaaa aaggaaaaat cctcctgcta ccaaggctaa
    22321 ccactcaaag atggcaaaga aaaatccgcc ctgactcacg taagtcagca aataataaag
    22381 caaaccttga cttccataat agtcgctgta aatcttccct gtctgatgaa gcgcccaacc
    22441 tgcataaaaa tcctgcactt cttgtgcact cattaagtcg agtaatagcg gtactcctag
    22501 agttatcccc gttacaagcg tactccatag taaaattttc accaaaggaa gacgacttga
    22561 ttcacgatga tgcgattctt gttcgatttg gtattctaga ggttcacgat tctccttatg
    22621 aacttcttct actctaccat acacactcat atcgtttctc ctgttcaatt tatctgtctt
    22681 agtatatcaa aaagtcctag gattgtcagc ttgcgatggc tgtttgagtg attttttgca
    22741 tagtttcaca aaggtttcaa tttcccgaaa gcggtgtaaa tgtgtctgtt tgaactcaat
    22801 catatcattc acttcttcct tagttagcat gaattcccct cctctttcta ttcgtaaaag
    22861 acaagaaaaa taggtctggt ttgtatccta agttctagat ttcttataag ataggggcga
    22921 atagctgggc gatttctttt acaagctttt gataccagct cgttttgata gaatgaggat
    22981 agacttcctg agaatctgca aatatatttc gaaaatctcg ggcgatttcc cttatagaag
    23041 gagttttata gagtaagact gcattttcat aatggtgtac caagctccgg tagtcgagat
    23101 tgattgtccc caccaccgca aaatcttcgt ctaccaacat ctgcttacta tgaataaaac
    23161 ctggactata ctcataaatc cgaacaccag cagaaagaag gtcgggataa gctcctctcg
    23221 tgactaactg aatgaacttc ttatctggta tgtaaggggt gataattcga acatcgaccc
    23281 ctctcatagc tgcatttttg attgtctctg ttaaatcata atctataatc aaataaggtg
    23341 tcgtaatata gaccgattct gttgcttgat tgattaaact ctgataaact tttttcccta
    23401 cctgcgctcg aaaaattggc ttgggtccac ttccgtatgg aatggttaaa ccgtcactcg
    23461 ggatagaatg attttctaaa tgatattgat caaaatcact aatttctcct cgattaatgt
    23521 accaagtggt caaaaataag cgtgtcagag cttttactgc tagtccgtct aagcgaattc
    23581 cactatcctt ccaataacca aatctctcga cgtggttaat gtactcatct gccagattga
    23641 ccccaccagt ataggctatc tgaccatcaa caatcaatat ttttctatga tctctgttat
    23701 tataagccac tgtcaaacga ggaataactt tattgaattt atgggcctcg atgcccagct
    23761 gacgaagtcg atgtgcataa tctcctgtta aagtagccat acagccgata tcatcataga
    23821 gcatcttaac ctctacaccc tgagctacct tttgctctag tatatctagt atgcgattcc
    23881 acatcaaacc ttcttctata atgtaatatt ccaagaaaat aaatttctca gcctttttaa
    23941 gatcttctac catctttttc cacatagctt ctccgttagg aaaaaatgta gaggcagttt
    24001 gatcatagat gtcagcattg gtatccatac tcaataagga cttgatgact ccataagctg
    24061 ccttgtcact ttcttttaat ttctctttta gtagctggct atttgcttct tggaaatgca
    24121 tagagcctag cttcttcagt tgtttgattt cttttttgga caatcgcctt tcaccaaaca
    24181 tcagatagag caagggacca aatactggca caaaggctac taacaaccag gttaccttat
    24241 tctcaggagt cgtattacgg ttgactatcg aaatgattgt gatgatactc agtagaatga
    24301 ggacagtaat ccataaaatg ggagccatgc gtcctaaata aagaaagaga ccaaaaacaa
    24361 gacaaagttc aagtaacata atcgaaagac taaagccata cttggacatc aataattgaa
    24421 atttcctata tttcatatcc cctccttgat agtttgaatg ctaaaaacaa gtaattgata
    24481 ctagtataac gcaggtattc ggtagaaggc aagtatttat gattattttc taggtaaaag
    24541 taaaaaggct gaaaaatcgg ctctataata tttgtagtgg gtaaatccac tatagatatt
//
LOCUS       DAVMEF010000011        24206 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMEF010000011 DAVMEF010000000
VERSION     DAVMEF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.011"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..303
                     /locus_tag="VYQ30_000318"
     CDS             <1..303
                     /locus_tag="VYQ30_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1965490.1"
                     /translation="EKPKPEVKPQLEKPKPEVKPQPEKPKPDNSKPQADDKKPSTTNN
                     LSKDKQPSNQASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(522..1502)
                     /gene="dusB"
                     /locus_tag="VYQ30_000319"
     CDS             complement(522..1502)
                     /gene="dusB"
                     /locus_tag="VYQ30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1965491.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1489..2361)
                     /gene="hslO"
                     /locus_tag="VYQ30_000320"
     CDS             complement(1489..2361)
                     /gene="hslO"
                     /locus_tag="VYQ30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1965492.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2526..3953
                     /locus_tag="VYQ30_000321"
     CDS             2526..3953
                     /locus_tag="VYQ30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1965493.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4112..5620
                     /gene="glpK"
                     /locus_tag="VYQ30_000322"
     CDS             4112..5620
                     /gene="glpK"
                     /locus_tag="VYQ30_000322"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1965494.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5662..7488
                     /gene="glpO"
                     /locus_tag="VYQ30_000323"
     CDS             5662..7488
                     /gene="glpO"
                     /locus_tag="VYQ30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1965495.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKRTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALAQNDLAELKN"
     gene            7558..8262
                     /locus_tag="VYQ30_000324"
     CDS             7558..8262
                     /locus_tag="VYQ30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1965496.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8493..8624
                     /locus_tag="VYQ30_000325"
     CDS             8493..8624
                     /locus_tag="VYQ30_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1965497.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            8645..10195
                     /gene="dltA"
                     /locus_tag="VYQ30_000326"
     CDS             8645..10195
                     /gene="dltA"
                     /locus_tag="VYQ30_000326"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1965498.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            10192..11436
                     /gene="dltB"
                     /locus_tag="VYQ30_000327"
     CDS             10192..11436
                     /gene="dltB"
                     /locus_tag="VYQ30_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1965499.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            11450..11689
                     /gene="dltC"
                     /locus_tag="VYQ30_000328"
     CDS             11450..11689
                     /gene="dltC"
                     /locus_tag="VYQ30_000328"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1965500.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            11682..12950
                     /gene="dltD"
                     /locus_tag="VYQ30_000329"
     CDS             11682..12950
                     /gene="dltD"
                     /locus_tag="VYQ30_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1965501.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            13105..13545
                     /gene="adcR"
                     /locus_tag="VYQ30_000330"
     CDS             13105..13545
                     /gene="adcR"
                     /locus_tag="VYQ30_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1965502.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            13545..14249
                     /locus_tag="VYQ30_000331"
     CDS             13545..14249
                     /locus_tag="VYQ30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1965503.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            14242..15048
                     /locus_tag="VYQ30_000332"
     CDS             14242..15048
                     /locus_tag="VYQ30_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1965504.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            15058..16563
                     /gene="adcA"
                     /locus_tag="VYQ30_000333"
     CDS             15058..16563
                     /gene="adcA"
                     /locus_tag="VYQ30_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1965505.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(17734..18507)
                     /locus_tag="VYQ30_000334"
     CDS             complement(17734..18507)
                     /locus_tag="VYQ30_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1965506.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
     gene            18683..20086
                     /locus_tag="VYQ30_000335"
     CDS             18683..20086
                     /locus_tag="VYQ30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1965507.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            20201..20839
                     /locus_tag="VYQ30_000336"
     CDS             20201..20839
                     /locus_tag="VYQ30_000336"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1965508.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            20851..21291
                     /locus_tag="VYQ30_000337"
     CDS             20851..21291
                     /locus_tag="VYQ30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1965509.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            21284..21715
                     /locus_tag="VYQ30_000338"
     CDS             21284..21715
                     /locus_tag="VYQ30_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1965510.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            21706..22176
                     /locus_tag="VYQ30_000339"
     CDS             21706..22176
                     /locus_tag="VYQ30_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1965511.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            22201..22974
                     /locus_tag="VYQ30_000340"
     CDS             22201..22974
                     /locus_tag="VYQ30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1965512.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            22971..23771
                     /locus_tag="VYQ30_000341"
     CDS             22971..23771
                     /locus_tag="VYQ30_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1965513.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            23830..>24206
                     /locus_tag="VYQ30_000342"
     CDS             23830..>24206
                     /locus_tag="VYQ30_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEV1965514.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAIS"
BASE COUNT         7196 a         4370 c         5368 g         7272 t
ORIGIN      
        1 gaaaaaccaa aaccagaggt taaaccgcag ctggaaaaac caaaaccaga ggttaaaccg
       61 cagccggaaa aaccaaaacc agataatagc aagccacaag cagatgataa gaagccatca
      121 actacaaata atttaagcaa ggacaagcaa ccttctaacc aagcttcaac aaacgaaaaa
      181 gcaacaaata aaccgaagaa gtcattgcca tcaactggat ctatttcaaa tctagcactt
      241 gaaattgcag gtcttcttac cttggcgggg gcaaccattc ttgctaagaa aagaatgaaa
      301 tagtgattga ttcattcaaa aatgtttgta aaaagtattc gacttaaaag aaaagagtct
      361 gggataaaaa gattttgatt ttaggaattc tttattataa atttttaaaa tcgatagatt
      421 agacaaaaaa gcgaacaaga cagaattctg agtgtcagat aactgatttt gttcgctttt
      481 tatatttaat attatacttt tgtcctaggc tcttttttgc attatacttt ttccaattgc
      541 aagagagctt caatctctgc tagggtgcta gcttgcgaaa tggctccacg gagtttggca
      601 gcgccagatg ttccacggag atagtgagga gcgaggccgc ggaattcacg aactgcgacg
      661 ttttctcctt tgaggttaat caatcgtttc aagtgttcgt aggcgatctt catcttgtct
      721 tcaaatgtca aatcaggtag gatttctcct gtttcaaagt aatggttgat ttggttgaag
      781 aggtaaggat ttcccatggc agctcggcca atcatgactg cgtcagcacc aacttcttcg
      841 atgcgttgct tggcttcttg gacagtacgg atatcaccgt tggcgatgaa tggaatcttg
      901 gttagagctt gggcaacctt gtaaagggtc tcaaggtccg cgtggccagt atacatttgt
      961 tcacgggtac ggccatgcat ggcgagggca gaaacacctg cagcctcagc agcgagggca
     1021 ttttctactg caagagatgg gtccgcccag ccggtacgca ttttgacagt aagtgggata
     1081 tcaaggacag actggacctt gttgatgata gagtagatct tgtcaggatc cttgagccac
     1141 atagcaccag cttcgttctt cacgattttg ttgacagggc agcccatgtt gatatcgacg
     1201 atatcggtct tggtgttttc ttggatgaat tctgctgcgc gtgctaggct gtcttcatcg
     1261 ctaccaaaaa gttggataga gacagggttt tcgccctcat cgatatgaag catgtgcagg
     1321 gttttttcgt tgttgtattg gattcccttg tcagagacca tttccattac aacgagtcca
     1381 gctccgagct cttttgctat ggtacgaaag gctgagttgg tcacgccagc cataggcgct
     1441 aaaacggtac gattgggaat ctcaatattg ccaatcataa aaggtgtatt aagatttgtc
     1501 acgaatgagt tcctccaggt ccttttcatc aaagttgtaa gtagtttggc agaattgaca
     1561 agtgatttct gcaccgtggt cttcctcttt catttcctgt aagtctgagc ttggaaggct
     1621 ggcaagagcg ttcataaagc gttcatggct acagtcacat tggaaacgga tttcttcttc
     1681 agaaagacgc ttgtaggctt cgtccccgta gatagccttg aggagggctt cgatatggtc
     1741 gtcgctttcg agaagagtag agatagctgg catttcttgg atgcgttttt caaagcgagc
     1801 aatctcttct ttcttggctc ctggcaagac ttgaactagg aaaccacctg caaccttgac
     1861 cttgtcttcc tcgtccaaaa ggacattgag gccgaccgct gaaggcgttt gttggctttc
     1921 agtaaggtaa aaggcaaggt cttcaccgat ttctccagag atgaggggag ttatagagtt
     1981 gtaaggattt ccagtaccgt agtctgtgat aacgaggaat tgaccatttc caacaaaagg
     2041 tccgactagg acttcaccag tcgcagtctt tttgatgtca acaccaggat tttgaacata
     2101 gcctttgacg ttccccttgg tatcagcgac ggtgataata gcacctagag agctagatcc
     2161 caacacctta actgtaagtt tggtatttcc tttttcattg gctgcgagaa tctggctagc
     2221 gataagagtt cgaccaagcg ctacagttga gctagcttgg gtttgatgtt tttcttgagc
     2281 agtgcggacg gtttcagtgc tatcaaggac aaaagcacga aaggctccgc tttctgatat
     2341 agttttaata attttatcca tagctactat tttagcataa aaatgcccaa agggggagcc
     2401 gtgtgtttac tgattttcag gataatggac caggaaatca gcatgaaaat aaaaagagaa
     2461 acagattatt ttagcatttg tcagatttat gctatgctta aggtagaaaa tgaaaggggt
     2521 aacaaatgta tttaggagat ttgatggaga aagccgagtg tggtcaattt tcaatccttt
     2581 cctttctatt acaagagtct cagacgaccg tcaaggctgt aatggaagaa acaggatttt
     2641 caaaagcaac cctaaccaaa tatgtcaccc tgctcaatga caaggctttg gatagtggct
     2701 tagagctgac tattcactca gaagatgaaa atctgcgtct gtctatcggt gcagctacca
     2761 aggggagaga tattcggagc ttgtttttgg agagtgctgt taaataccag attttggttt
     2821 atcttctcta ccaccaacag tttttagccc atcagctggc tcaagaattg gtgattagcg
     2881 aggctacgct tggtcgtcac ttggctggtt taaatcagat tttgtcagaa tttgatttat
     2941 ccatccaaaa tggacgttgg cgaggtccag agcatcagat tcgctatttc tatttctgtc
     3001 ttttccgaaa ggtctggtcg agtcaggaat gggaaggtca catgcagaaa ccagagagaa
     3061 aacaggagat tgccaattta gaggaaatct gcggtgcaag tttgtctgcg gggcagaaat
     3121 tggacttggt tctctgggct cacatcagtc aacaacgtct tcgggtcaat gcttgtcagt
     3181 ttcaagtcat agaagagaaa atgcgagggt attttgacaa tatcttttat cttcgtttgc
     3241 tgagaaaggt tccgtccttt tttgctgggc aacatattcc actaggagtt gaggatggtg
     3301 agatgatgat attcttctct tttctcctat ctcatcgcat tcttcctctt catactatgg
     3361 agtatattct tggttttgga gggcagttgg cagatttact gacgcaattg attcaagaaa
     3421 tgaagaagga ggaactattg ggggattata cagaggacca tgtcacctat gaactcagtc
     3481 agctttgtgc tcaagtctat ctctataagg gctatatttt acaggatcgc tacaagtacc
     3541 agttagagaa tcgtcatcca tatttactga tggaacatga ttttaaagag acagcagagg
     3601 agatttttca tgctctgcct gcttttcaac aggggacaga tttagataag aagattctct
     3661 gggaatggct ccagttaatc gaatatatgg ctgaaaacgg tggccagcat atgcggattg
     3721 gtctggattt gacatctggt tttcttgtct tttcaaggat ggcagccatt ttgaaacggt
     3781 atttggaata caatcgtttt attaccattg aagcttatga ccctagtcgg cattatgatt
     3841 tgctggttac caataacccg attcataaga aggaacagac accagtctat tatttaaaaa
     3901 acgacttgga tatggaagat ttggcaggga ttcgtcagtt attattcact taaaaggctt
     3961 ggttaatcca ggtctttttt gtgaaattca cacaatctcc tcacattttt ttaaaaatta
     4021 aaaaaagttg ataaacaaga aagcgcttta ttttgtatac tagtaagtgt aaagaggaaa
     4081 cacctcaaga tctttatcag gaggacagta tatgtcacaa gaaaaataca tcatggccat
     4141 tgaccaggga actacaagtt ctcgtgccat cattttcaac aaaaaagggg aaaaggttag
     4201 ctcgagtcaa aaagagttta cccagatttt ccctcaggca ggttgggttg agcacaatgc
     4261 caatgaaatt tggaactctg ttcagtcagt tattgcgggt gctttcatcg aaagtggtgt
     4321 caagccaaat caaatcgagg caatcgggat taccaaccaa cgtgaaacaa cggttgtctg
     4381 ggataagaaa acaggacttc ctatctacaa tgctatcgtt tggcagtcac gccagacagc
     4441 acctttggct gagcaactaa aaagccaagg ttatgtggaa aaattccatg aaaagactgg
     4501 tttgattatt gatgcttact tctctgctac caaggttcgt tggattttgg atcatgtaga
     4561 aggtgctcaa gagcgagcag aaaaagggga attgctcttt ggtactatcg atacttggtt
     4621 ggtttggaaa ttgactgacg gtgcggctca cgtgactgac tactcaaatg cagctcgtac
     4681 catgctttat aacattaaag aactcaaatg ggatgatgag attttggaaa tccttaacat
     4741 tccgaaggct atacttccag aagttcgttc taactccgaa atctacggca agacagctcc
     4801 attccatttc tacggtggag aggtgccaat ctcaggtatg gctggggacc aacaagcagc
     4861 cctctttgga cagttggctt ttgagccagg tatggttaag aatacttatg gaacaggctc
     4921 tttcatcatc atgaatactg gggaagagat gcagttgtct gaaaacaacc tcttgacaac
     4981 cattggttac ggaatcaacg gtaaggttta ttatgccttg gaaggttcta tcttcatcgc
     5041 aggaagtgct attcagtggc ttcgtgacgg tcttcgcatg gttgaaaatt caccagaatc
     5101 tgaaaaatac gctcgtgatt ctcacaacaa cgatgaagtt tatgtcgttc cagcctttac
     5161 aggtctaggc gctccatact ggaaccaaaa tgctcgtggt tccgtctttg gtttgactcg
     5221 tggaacaagc aaagaagact ttatcaaggc gactttgcaa tctattgctt atcaagtgcg
     5281 tgatatcatc gacaccatgc aagtggatac tcagaccgcc attcaagtac tgaaggtgga
     5341 tggtggtgca gccatgaaca acttcctcat gcagttccag gcggatattt taggcattga
     5401 cattgcacgt gctaaaaacc tggaaacaac agctctagga gcggccttcc tagcaggttt
     5461 gtcagtaggg tactggaaag acttggacga gttgaaactc ttgaacgaga caggagaact
     5521 ctttgagcca tctatgaacg aatctcgcaa ggaacaactc tacaagggct ggaagaaggc
     5581 tgtgaaagca actcaagtct ttgcggaagt agacgactaa tactggcaga ataaagcgat
     5641 ttatttagaa agtgtgtaaa tatggaattt tcaaagaaaa cacgtgaatt gtcaattaaa
     5701 aaaatgcagg aacgcaccct ggacctcttg attatcggtg gaggaatcac aggagctggt
     5761 gtagccttgc aggcggcagc tagcggtctt gagactggtt tgattgaaat gcaagacttt
     5821 gcagaaggaa catctagtcg ttcaacaaaa ttggttcacg gaggtcttcg ctacctcaaa
     5881 caattcgacg tagaagtggt ctcagatacg gtttctgaac gtgcagtggt tcaacaaatc
     5941 gctccacaca ttccaaaacc agatccaatg ctcttaccag tttacgatga agatggagca
     6001 acctttagcc tcttccgtct taaagtagcc atggacttgt acgacctctt ggcaggtgtt
     6061 agcaacacac caactgcgaa caaggttttg agcaaggatc aagtcttgga acgccagcca
     6121 aacttgaaga aggaaggctt ggtaggaggt ggagtgtatc ttgacttccg taacaacgat
     6181 gcgcgtctcg tgattgaaaa catcaaacgt gccaaccaag acggtgccct cattgccaac
     6241 cacgtgaagg cagaaggctt cctctttgac gaaagtggca agattacagg tgttgtagct
     6301 cgtgatctct tgacagacca agtatttgaa atcaaggccc gtctggttat taatacaaca
     6361 ggtccttgga gtgataaagt acgtaatttg tctaataaga gaacgcaatt ctcacaaatg
     6421 cgcccaacta agggagttca cttggtagtg gattcaagca aaatcaaggt ttcacagcca
     6481 gtttactttg acacaggttt gggtgacggt cgtatggtct ttgttctccc acgtgaaaac
     6541 aagacttact ttggtacaac tgatacagac tacacaggtg atttggagca tccaaaagta
     6601 actcaagaag atgtagatta tctacttggc attgtcaaca accgcttccc agaatccaac
     6661 atcaccattg atgatatcga aagcagctgg gcaggtcttc gtccattgat tgcagggaac
     6721 agtgcctctg actataatgg tggaaataac ggtaccatca gtgatgaaag ctttgacaac
     6781 ttgattgcga ctgttgaatc ttatctctcc aaagaaaaaa cacgtgaaga tgttgagtct
     6841 gctgtcagca agcttgaaag tagcacatct gagaaacatt tggatccatc tgcagtttct
     6901 cgtgggtcta gcttggaccg tgatgacaat ggtctcttga ctcttgctgg tggtaaaatc
     6961 acagactacc gtaagatggc tgaaggagct atggagcgcg tggttgacat cctcaaagca
     7021 gaatttgacc gtagctttaa attgatcaat tctaaaactt accctgtttc aggtggagaa
     7081 ttgaacccag caaatgtgga ttcagaaatc gaagcctttg cgcaacttgg agtatcacgt
     7141 ggtttggata gcaaggaagc tcactatctg gcaaatcttt acggttcaaa tgcaccgaaa
     7201 gtctttgcac ttgctcacag cttggaacaa gcgccaggac tcagcttggc agatactttg
     7261 tcccttcact atgcaatgcg caacgagttg gctcttagcc cagttgactt ccttcttcgt
     7321 cgtaccaacc atatgctctt tatgcgtgat agcttggata gcatcgttga gccagttttg
     7381 gatgaaatgg gacgattcta tgactggaca gaagaagaaa aagcaactta ccgtgctgat
     7441 gtcgaagcag ctctcgctca aaacgactta gcagaattaa aaaattaaga aaaaataaaa
     7501 gaggtggagg gcagcattcc ttgtcgcccg tcccttcttt ttaatggaga cagaaagatg
     7561 atgaatgaat tatttggaga atttctaggg actttaatcc tgattcttct aggaaatggt
     7621 gttgttgcag gtgtggttct tcctaaaacc aagagcaata gctcaggttg gattgtgatt
     7681 actatgggtt gggggattgc agttgcggtt gcagtctttg tatctggcaa gctcagtcca
     7741 gctcatttaa atccagctgt gacagttggt gtagctttaa aaggtgactt gccttgggct
     7801 tccgttttgc cttatatctt agcccagttc gcaggggcca tgctgggtca gattttggtt
     7861 tggttgcaat tcaaacctca ctatgaggca gaagaaaatg caggcaatat cctggcaacc
     7921 ttcagtactg gaccagccat caaggatact gtatcaaact tgattagcga aatccttgga
     7981 acctttgttt tggtgttgac aatctttgct ttgggtcttt acgattttca ggcaggtatc
     8041 ggaacctttg cagtgggaac tttgattgtc ggtatcggtc tatcactagg tgggacaaca
     8101 ggttatgcct tgaacccagc tcgtgacctt ggacctcgta tcatgcacag catcttgcca
     8161 attccaaaca agggagacgg agactggtct tacgcttgga ttcctgttgt aggccctgtt
     8221 atcggagcag ccttggcagt gcttgtattc tcactttttt agtttagaaa ataactatta
     8281 ttgctagagc ttgagatgaa aatctcaggc ttttttcttt tgaaatagat atagaaaaac
     8341 cgcataatcc ttaaaataag ctgaaatcta ttggaaacat acggtaaaca attgaaaaga
     8401 tactatataa agtggtacaa tggtagaaaa ataagctagt aagaatcact cttattttag
     8461 cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca gagaaaagaa ttgtacctct
     8521 ttcttggtcg gacagccttg tattttctta tctttctagg gctgctttac ttctttagct
     8581 atcttggtca gggtcaagga agctttatct ataatgaatt ttaacttgaa ggagaatccc
     8641 tattgtgtca aataaaccaa tagcagatat gattgaaacc attgagcatt ttgctcagac
     8701 acagcctagc tatcctgtct ataatgtttt ggggcaggaa cacacttatg gcgatttaaa
     8761 ggctgattcg gatagtttgg ctgcagtcat tgaccaacta ggcttgcctg agaagtctcc
     8821 tgtggttgtt tttggtggcc aagaatatga aatgttggca acctttgtag cgctgactaa
     8881 gtcaggtcat gcctacattc caattgatag ccattcggcc ttggagcgag tttcagctat
     8941 tttagaagta gcagagccaa gcttgattat tgccatttca gcctttccct tggagcaggt
     9001 ttctacacca atgataaatc tagctcaggt tcaagaagcc tttgcccaag ggaataacta
     9061 tgaaatcacg catccagtca agggagatga taattactac attatcttta cttctggtac
     9121 gactggtaag cctaagggag tgcagatttc acatgataat ctcctcagct ttacaaactg
     9181 gatgattacg gataaggaat ttgcgacacc gagtcgtccg caaatgctgg cacagccacc
     9241 ttattctttt gacttgtctg tcatgtattg ggcaccgacc ttggcacttg gtggtacgct
     9301 tttcactctt ccttcagtca tcactcagga ctttaagcaa ctctttgcgg ctatcttttc
     9361 attgccaatc gctatctgga catcaacacc atcctttgca gatatggcca tgttgtctga
     9421 atacttcaac agtgagaaaa tgcctggaat cacgcatttc tactttgatg gtgaagaatt
     9481 gacggtcaaa acagctcaaa aactgcgcga gcgtttccca aatgcccgta tcatcaatgc
     9541 ttacggccca acagaagcga cagtagctct gtcagcagtt gccgtgacag acgagatgtt
     9601 agcgactctc aaacgcctac caatcggcta taccaaggct gattctccaa cctttatcat
     9661 tgacgaggaa ggaaataaac tgccaaatgg tgagcaggga gaaatcattg tttctgggcc
     9721 agctgtttca aaaggttata tgaacaatcc tgaaaaaaca gcagaagcct tctttgagtt
     9781 tgaagatctg ccagcctatc acacaggcga tgtgggaacc atgacagatg agggcttgct
     9841 tctctacggc ggacgcatgg acttccagat taagtttaac ggttaccgca ttgagttaga
     9901 agatgtctct caaaacctca acaagtctcg ctttatcgaa tctgctgtcg cagtaccgcg
     9961 ctataacaag gaccacaagg tacaaaatct attggcttat gtcatcttaa aagacggtgt
    10021 tcgtgagcag tttgagcgag atatcgatat taccaaggcc atcaaggaag acctgacaga
    10081 catcatgatg tcctatatga tgccatctaa attcctttac cgagacagtt tgccactgac
    10141 tccaaatgga aagattgaca tcaaaggatt gattaacgag gtgaataaga gatgatggag
    10201 ttttttcaac agcttcctca tttagagcca tatggcaatc ctcagtattt tgtttatgtg
    10261 attgctgcaa ccttgcccat ctttataggc ctctttttca agaaacgctt tgcctggtat
    10321 gaagtgttgg taagtctctt ctttattgtc accatgttgg tgggtggaaa gaccaatcaa
    10381 ctagctgcct tgggtattta cctttgctgg gaaatattgc tcctgctttt ctacaagcat
    10441 tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag ttagttttct gtccctactt
    10501 ccgattatct ttgtcaaggt gcaaccagct atcaatggaa cgcagtcttt gcttgggttc
    10561 ttgggaattt cttacctgac ctttcgttcg gttgggatta tcatcgagct gagagatgga
    10621 gtgattaagg attttaccct ctgggaattt ctccgtttcc ttctcttcat gccaactttc
    10681 tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa attatcaggc tattcctgag
    10741 cgagatgagt tgatggatat gctggatgaa tctgtccgct atatcatgcg gggctttttg
    10801 tataagttta tcctagctca tgttttagga gagaccttac tacctcctct gaagaattta
    10861 gccttgcagt caggtggctt ctttaatctc tatgccttgg cagttatgta tacttttggt
    10921 ctggagctct tctttgactt tgcaggttat tctatgtttg ctttggccat ctcaaacttg
    10981 atgggaatcc gtagccctat caactttaac aagccctttt tatcaaggga tttaaaggag
    11041 ttttggaatc gctggcatat gagtctgtcc ttctggttcc gtgactttgt ctttatgcga
    11101 atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc gtaatgtaac ctcaagcatg
    11161 gcctacattg taaatatgct gattatggga ttttggcatg gtgtgacctg gtactatatc
    11221 gcctatggac tctttcatgg actaggcttg gtcatcaatg atgcctgggt tcgcaagaaa
    11281 aaaacgctca ataaggaacg gaaaaaagca gggaaggctg ccctacctga gaatcgctgg
    11341 attcagttgc ttggcatggt tgtcactttc catgttgtca tgttgtcatt cttaatcttt
    11401 tctggattct tgaataatct atggtttaaa aaataaaagg aaataaaata tggatatcaa
    11461 atcagaagtt attgaaatta ttgatgagtt gtttatggaa gatgtttctg acatgatgga
    11521 tgaagatctt tttgatgcag gtgtcttgga tagtatggga acggttgagt tgattgtgga
    11581 gattgagaac cgttttgaca ttcgtgtccc tgtaacagag tttggtcgtg acgactggaa
    11641 tacagctaat aaaatcatag ctggtattgt ggagctacaa aatgcttaaa cgcttatgga
    11701 tgatcttcgg accggtcttg atcgctggtt tgttggtttt tctgctcatt ttcttttatc
    11761 ctactgagat gcatcataat ctaggagctg aaaagcgttc agcagtggct actactatcg
    11821 atagttttaa ggagcgaagt caaaaagtca gagcactatc tgatccaaat gtgcgttttg
    11881 ttcccttctt tggctctagt gaatggcttc gttttgacgg tgctcatcct gcggtattag
    11941 ctgagaaata caatcgttcc taccgtcctt atcttttagg acagggggga gctgcatcgc
    12001 ttaaccaata ttttggaatg caacagatgt taccacagct ggagaataaa caagttgtgt
    12061 atgttatctc acctcagtgg ttcagtaaaa atggctatga tccagcagcc ttccagcagt
    12121 attttaatgg agaccagttg actagttttc tgaaacatca atctggggat caggctagtc
    12181 aatatgcagc gactcgctta ctgcaacagt tcccaaacgt agctatgaag gacctggttc
    12241 agaagttggc aagtaaagga gaattgtcga cagcagacaa tgaaatgatt gaattattgg
    12301 ctcgttttaa tgaacgccaa gcctcctttt ttggtcagtt ttcggttaga ggctatgtta
    12361 actacgataa gcatgtagct aagtatttaa aaatcttgcc agaccagttt tcttatcaag
    12421 caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac ttccaataat gagatgggaa
    12481 tggaaaatta tttctataat gagcagatca agaaggattt gaagaaatta aaggattctc
    12541 agaaaagctt tacctatctc aagtcgccag agtataatga cttgcagttg gttttaacac
    12601 agttttctaa atctaaggta aacccgattt ttatcattcc acctgttaat aaaaaatgga
    12661 tggactatgc tggtctacga gaggatatgt accaacaaac ggtgcagaag attcgctacc
    12721 agttagaaag tcaaggtttt accaatatag cagatttttc taaggacggc ggggagcctt
    12781 tctttatgaa ggacaccatt caccttggtt ggttgggttg gttggctttt gacaaggcag
    12841 ttgatccttt cctatccaat cccacaccag ctccgactta ccatctgaat gagcgctttt
    12901 tcagcaaaga ttgggcgact tatgatggag atgtcaaaga atttcaatag ataatataat
    12961 atagaagagt tcaagaatga aactagtttt cacgtttttt cttgactctt tttttgcttg
    13021 tgatataatt aactggtaaa caaaatgtca aagaatagta tttttctctt aaaaaagaga
    13081 agtctaaaag gaaaggagtc agatatgaga cagctagcaa aggatatcaa tgcttttttg
    13141 aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc taataggtca ttgcactagc
    13201 gaggtggccc tgaccaatac tcaggagcat atccttatgc tcttgtcaga ggaatcttta
    13261 acaaattcag aattggcccg tcgtctcaat gtcagtcagg cggcagttac caaggccatt
    13321 aagtctttgg tcaaggaagg gatgttggaa acatctaaag attctaaaga tgcgcgtgtg
    13381 attttttatc agttgactga cttggctcgt ccaatcgctg aggagcacca ccatcaccat
    13441 gagcatacac ttttaaccta tgaacaagtg gcgactcagt ttactccaaa tgaacaaaaa
    13501 gtgattcagc ggtttttgac tgctttagta ggagaaatca aataatgaga tatattacgg
    13561 tagaggattt gtccttctat tatgataagg agcctgttct tgaacatatc aattatagtg
    13621 ttgatagtgg ggaatttgtt accttgactg gggaaaatgg agcggctaag acgacgctca
    13681 tcaaggccag tcttggaatt ctgcaaccac gcattggaaa ggtggctatt tcaaagacaa
    13741 atacgcaagg taagaaattg agaatagcct atcttcctca acaaattgcc agttttaatg
    13801 ctggttttcc aagtacggtc tatgaatttg tcaagtcggg tcgctatccg agaaaaggct
    13861 ggttccgtcg tttgaatgct catgatgagg agcatatcaa ggctagtctg gactcagttg
    13921 gcatgtggga acatcgagac aaacgcttgg ggtctctatc tgggggacaa aagcagcgag
    13981 cggtgattgc gcgtatgttt gcttctgacc ctgatgtgtt tatcctagac gagccgacaa
    14041 cggggatgga tgcaggaagt aaaaatgaat tttacgaact catgcaccac agcgcccatc
    14101 atcatggcaa ggctgttttg atgattaccc atgaccctga agaagttaag gattatgcgg
    14161 atcgcaatat tcatctagtc cgtaaccaag actcgccatg gcgttgtttc aatgttcatg
    14221 agaatggcca ggaggtgggc catgcttagt ttattatctt acgactttat acaacgcgcc
    14281 tttttggcgg ttattgctat gagtcttttc tcaccggtat tgggaacctt cctcatcttg
    14341 cgtcgtcaga gtttgatgag tgataccctt agccacgtct cactttcagg tgtagccttt
    14401 ggtctggttt tggggatttc tccaactgtt tctactattg ccattgtctt gattgcggcg
    14461 gtctttctgg agtatctccg tacggtttac aagagcttta tggaaatcgg gacagctatc
    14521 ctcatgtcaa caggtctggc tgtttctctg attgtcatga gcaagggtaa aagctcgagt
    14581 tcaatgagtt tggaccaata tctctttggt tcgatcgtga ctatcagtga agaacaggtc
    14641 atttccctct ttgtcattgc ggcggttgtt ttgattttga cctttctctt tcttcgtcct
    14701 atgtatatct taacttttga cgaagatacg gcctttgtgg atggcttgcc agttcgtacc
    14761 atgtccattc tttttaacat ggtgacaggg gtggctattg cccttatgat tcctgcagca
    14821 ggagctcttc tggtatcgac cattatggtc ttgccagcta gtattgccct gcgtctgggg
    14881 aaaaacttta aatcggttat gctgcttgcc agtgcgattg gctttttggg aatggtagca
    14941 ggactttaca tttcctacta tgcagaaaca cctgcaagtg caagtattac cattattttt
    15001 gtaactgtct ttatactaat cagtttagta agacgtttta tcaaatagga gacgaacgtg
    15061 aaaaaaatta gcttattact agccagtcta tgtgccttgt ttttagtggc ttgttccaat
    15121 caaaaacagg cagatggtaa actcaatatc gtgacaacct tttaccctgt ctatgaattt
    15181 accaagcaag ttgcaggaga tacggctaat gtagaactcc taatcggtgc tgggacagaa
    15241 cctcatgaat acgaaccatc tgccaaggca gttgccaaaa tccaagatgc agataccttc
    15301 gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat tgctagatac cttggataag
    15361 aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc tcttgccagg tggcgaggaa
    15421 gaagagggag accatgacca tggagaagaa ggtcatcacc atgagtttga cccccatgtt
    15481 tggttatcac cagttcgtgc cattaaacta gtagagcaca ttcgtgacag cttgtcagca
    15541 gattatcctg ataaaaaaga gacctttgag aagaatgcag ctgcctatat cgaaaaattg
    15601 caagccttgg ataaggctta cgcagaaggc ttgtctcaag caaaacaaaa gagctttgtg
    15661 actcaacacg cagcctttaa ctatcttgcc ttggactatg gactcaaaca agtcgcaatc
    15721 tcaggccttt ctccagatgc agagccatca gctgctcgct tggcagaatt gacagagtat
    15781 gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa atgcctcaca agcccttgct
    15841 aacacacttt caaaagaagc aggtgtcaaa actgatgtcc tcaatccttt agaaagtctg
    15901 acagaagagg acaccaaggc tggagaaaac tacatttccg tgatggagaa aaacctcaag
    15961 gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg aacctgaaaa ggcagaggat
    16021 accaagacag tccaaaatgg ttacttcgag gatgcagctg tcaaggaccg caccttgagt
    16081 gactatgcag gtaactggca atcagtttat cctttccttg aagacggcac gtttgaccaa
    16141 gtctttgact acaaggctaa gttgactggt aagatgaccc aggctgagta caaggcttac
    16201 tatacaaaag gctatcagac agatgtgact aagattaaca ttactgataa tactatggaa
    16261 tttgttcaag gtggacaaag caagaaatac acttacaagt atgtcggtaa gaaaattttg
    16321 acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg aagccacaga tgctgacgct
    16381 ggacaattca agtatgttca gtttagtgac cacaatatcg ccccagttaa ggcagaacat
    16441 ttccatatct tttttggagg tacaagccaa gaaactctat ttgaagaaat ggataattgg
    16501 ccaacctact acccagataa cctatctggc caagaaatcg cccaagaaat gttggcgcat
    16561 tgatgagaaa tcgactagtt cataagagct agtcggtttt ttgatactag atggctaact
    16621 tcattataga cctttcagaa ctaagggaac ctgaaaaaat aaataaaact cttgaaatat
    16681 ctgggcttat atgctagaat gtagttagtg tataaggttc attagaacac caaagcccct
    16741 aactagtgcc atagttaagg gcttttttga cgtatcttag cgatttaacg ggtgtttata
    16801 ggctagtcag tcggtctact tcttaccatc tagccatttg cagacgaaat acaagataat
    16861 tccagctata acatctgcta taatagtaag agcaagctct ggtataaggc tcattagttt
    16921 cacctcctct cacgaaccca taggaacgta atcggtaacc gatgacaaaa atagtatacc
    16981 acaatacatt tagatcatca aggtcactta attcttgaac tattgtagat tatatgctaa
    17041 aatgtagtta attgataagg ttcattagaa caccaaagcc cctaactatg gccgtagtta
    17101 agggcttttt tgacgtatct tagcgattta acgggtgttt ataggctagt cactcggtct
    17161 acttcttact atctagccat ctgcagacga aatacaagat aattccagct ataacatccg
    17221 ctataatagt aagagcgagc tctatgataa ggctcattag tttcacctcc tctcacgaac
    17281 ccataggaac gtaatcggta accgatgaca aaaatagtat accacaatac atttagatca
    17341 tcaaggtcac ttaattcttg aaatataggg tctaggcgac aaagtcttta aaatcagaaa
    17401 aacgtataat atcaggtgtg caaaaacttg atactatgcg ttttattgtg ggaaggttta
    17461 ctccattttc tcctgaaatt gagtttttgt ccagcctctg tttttagggt tgctgagaaa
    17521 ataatgtcat gtggtgaata tttgtaaatc agtcagcaga cagaacgaat actcttcgaa
    17581 aatctcttca catcatgtca gcttcgtctt tccgtatata tgtgactgac ttcatcagtt
    17641 ctatctacaa cctcaaaaca gtgtttcgag ctgacttcat cagttttcaa atctgtactt
    17701 tgagcaagct gagactagct tcctatttga ttttcattga atatcagaaa cccattctcc
    17761 atcaaataat tcgactgcgt ctaataattt ttgatttggc aaggtgtcag aaataaaggt
    17821 tgtgtatttg gagaggggat taattttaaa aaatccagtc ttgtaaaatt tagaactatc
    17881 aatcagtaag atggtttcat gggctttgtc aataatattc ttttttgaaa tagcttggct
    17941 gagagaagct tcataaacat attggtcatc aatacctctt gctgaacaaa atgctaaatc
    18001 gatattaaaa tgatctaata aagaattttc cttatcatag ttgaccacgg aacaggattg
    18061 atgtttgacc tcgccagatg tgataaagat tttggagcta tctttaacag tttcagatag
    18121 ggtttgtgca gtatgtaaac catttgtaaa aataatcaaa ttatcaagtt cagaaagata
    18181 gggacaaagt tcgtagacag tcgtactgga atccagataa atacacatgc ctgaccgaat
    18241 aaagtcttta gcgagactag cgattagtct tttttgccta gtactttctc cttcccgtat
    18301 ttgatgagaa agttcaattg tgttcataga ggacagggtc acgtatccgt gctttctttt
    18361 gataagacct tgattttcta agaaaattaa atcacgacgt aaggtacttg tgctggagaa
    18421 agtgatttct gccagctctt ttacggcaat tctttttttc tttttgatga tttcaatcaa
    18481 ttcaagtaca cgttcatctt ttatcataag ctcctcctaa tttatcattt caactatatt
    18541 atagcacaaa ttggaggaat ttgaattatt tttatgaata ttgggttaac atttgaacat
    18601 tattcaagta agcgttcaca tattgaaaaa ataaaacgtg gggattataa taaagttaat
    18661 caaggacgaa gagagaagaa aaatggaagc ggttttagca atagatttag gtgcgacttc
    18721 tggaagagca atcgttggtt acctttctga aaataaacta gtaatggaag aaataaatcg
    18781 cttttctaat ctacctatta gagtaaaagg gcatttatct tgggatattg actttctact
    18841 agctaaaatt cttgaaagta tccgcttggc taatactagt tacaagattt tatctatcgg
    18901 tattgacaca tggggagttg attttggact ggttgataat gaaggtaagc tgttattaca
    18961 acctgttcat tatcgtgatg aaagaacaaa gggagtgtta aaggaaatat ctgaaatgac
    19021 tgaattagaa aaactgtatt cagagacagg aaatcagatt atggagataa ataccttgtt
    19081 tcaactcttt aaggcacgtc aagaatctcc tgactctttc tataagacca ataagattct
    19141 tttaatgcca gatttgttta attatctctt gacaggtaag tttgctacag aaaaaagcat
    19201 tgcttcaaca actcaattat ttgatcctag gagtcaaaac tggaatcaga atatcttaaa
    19261 actatttgaa ttggattcat ctttacttcc tgagattgtt tcagagggaa atgttcttgg
    19321 aaggataaaa aaggagtatg gtttaggcga tattcctgtt gtgaatgttt gtagtcatga
    19381 tacagcaagc gcgattgtct cagtacctaa gacagaaggt agtttattta tttcatcagg
    19441 tacttggtct ttggttggag tggaacttac ttcaccgctt cttactaccg aatccttcag
    19501 ttatggattt acaaatgaag tcggtaaaga tggagtgatt acatttctga agaattgtac
    19561 agggttgtgg atcatagagg aactaagacg ttcatttgaa cgaagaggga aagcctattc
    19621 ttttgatgat attaggacaa tggtggagaa agaaaaagaa aatcttcctc tgattgatac
    19681 tgaatcaact gaatttgcaa cagaatctga tatgcacaag actttgacag aatatctagc
    19741 ttatcatcat gaaactagag agtggacaga tggacaacta tttaagattg tttatgaaag
    19801 cctagctgaa acgtatagga aagcgataga gttactagaa gaactaactc ataaggttta
    19861 taagaggata tatgtgattg gaggaggtgc tagagccagt tactttaacc aaatgattgc
    19921 tgatagaact ggtaaagagg ttcttacagg tttgactgag gctacagctg tggggaatat
    19981 tgttgtgcag ctcatagcta tgggacaatt aaaagggatg gaagaagctc accatgttat
    20041 tgaggagttt ctacaattag agagttatta ctcccaaaag aattaaaaag attgagagtt
    20101 tgtaaatttg cctccctccc ccttcttaag ttttgtacaa gaagggggga taattggtga
    20161 attgaaaaat atttagtgtt ttgatatgag gaggacaaga atgtcagatg taaaacaaga
    20221 attaattaag tatggcaaga agctagtaga aacagatttg acgaaaggaa caggtgggaa
    20281 tctcagtgtt ttcgatcgtg aaaaacaatt gatggcaatt accccgtcgg gtattgattt
    20341 ctttgaaatc aaagaatccg atattgtagt gatggatatt aatggaaatg ttgtagaggg
    20401 agaacgcttg ccatctagcg aatggtatat gcatttgatt caatatcaaa ctcgtgatga
    20461 tatcgatgca attatccatg ctcatacaac ttatgcaaca gtattagctt gtctcagaga
    20521 accacttccg gctagtcatt atatgattgc agtggcaggg aaagatgttc gggtagctga
    20581 gtatgcaaca tatggtacga aagaattggc tgtgaatgca gctaaagcaa tggaaggtcg
    20641 tagagcagtt ttactagcga atcatggaat tttagcaggt gcacaaaatt tattgaatgc
    20701 atttaatatt gttgaagaag ttgaatattg tgcaaaaatt tattgtttag ctaagaattt
    20761 tggagagcca gtagttcttc ctgatgagga gatggattta atggcagaaa aatttaaaac
    20821 atacggtcag agaaaatagg gaggatatta atgttaaaac atataccgaa aaatatttct
    20881 ccagatttat tgaagacttt aatggaaatg ggacatggag atgaaatagt attagctgac
    20941 gcgaattatc cttctgcctc atgtgcaaat aagctaattc gttgtgatgg cgtaaatatt
    21001 ccagaattat tagattccat tctgtattta atgccattag atagttacgt cgatagttca
    21061 attcagttta tgaacgttgt ttcgggtgat gatattccta agatatgggg tacctataga
    21121 cagatgattg aaggtcatgg tacagatctt aaaacgatta cttatcttag aagagaagac
    21181 ttttatgaac gtagtaagaa agcttatgct attgttgcta caggagaaac ttcactttat
    21241 gctaatatta tccttaagaa aggagtagtt gttgaaagag aaaatgttta atagaggaat
    21301 tttagttgcc agtcatggta attttgctag cggagctctc atgaccgcag aaatgtttgt
    21361 tggtgagaca acaaatgata gagttaggac attaggtttg atgcctggag agaatattgt
    21421 agagtttgaa cattatttta aaaatcaagt aaatgaactg ttagactcaa atcaagaggt
    21481 tatcgttttg actgacttga ttggaggaag tcctaataat gtggctttgt cacggttttt
    21541 aaatttggat tcagttgata ttgtaacagg gtttaatatc cctctcctag tggaattaat
    21601 atcaagttat gattcaaaaa tcaatttaga agaaattgtt caaaatgctc aaaatagttt
    21661 gtttaatgtt aaacaacaac ttaacgtaga ggaggaagag gatttatgtc tatagagttt
    21721 gttcgtattg atgaccgtct ggtacatggt caagttgtca ctacgtggct aaaaaagtat
    21781 gatattgagc aagttatcat tgttaatgat cgcatctcag aagataaaac acgacaatct
    21841 attttaaaga tttctgcacc ggtaggttta aaaattgttt tctttagtgt aaaacggttt
    21901 gtggaagttt taaactctgt gccaataaaa aagagaacaa tgctgatata tacaaatcca
    21961 aaagatgtgt atgattctat tgaaggaaat ttaaaattgg agtacctcaa tgtaggacag
    22021 atgagtaaaa cggaggaaaa tgaaaaggta acgggaggtg tagctctagg tgaagaagac
    22081 aaatattatt ttaagaaaat agttgataag ggaacgagag ttgaaattca aatggttcct
    22141 aatgataaag ttacaatgtt ggaaaaattt ttataaaaat aatttaagga ggtacagtat
    22201 atgctattca cacaagcatt actggtgaca ttagttggga ttattgccac tattgactat
    22261 aatggaccgt tatttatgat tcaccgtccg ttagttacaa gtgcaatggt tggcttagtc
    22321 ttaggagatt tcacccaagg tgttcttatt ggttcagctc ttgaattaac ttggctcggt
    22381 gtaacaggta ttggaggtta cactccacca gatactattt caggtgcgat tattggtact
    22441 gcatttggta ttttatctgg tcaaggagaa actgctggta tcgctatagc agttccaatt
    22501 gcagttgcta cccaacagtt ggatgttctt gcaaaaactt tagatgttta ttttgtgaaa
    22561 aaagctgata atgatgctaa aaacggagat tattcaaaga tcggttttta tcattattca
    22621 agtttggttt taatcacgtt atttaaaatt gtaccaattt tcctagctat tatgcttgga
    22681 ggggaatatg tggcagactt gtttgctaag gttccaccaa tcgttatgca gggacttaac
    22741 tctgcaggtg ctttactacc ttcaattggt tttggtatgc ttttaaatat gatgctcaag
    22801 aaaaatatgt gggtattctt gttgattgga ttcatttgtt ctgtgtatgg aggaatgtca
    22861 accattggga tctcactagt tggtattgcg gtagcatact tctacgatat gattggaagt
    22921 aaaccacaag aaacaacttc aagtagtgat gttgaggagg atcttgatct atgatgaata
    22981 ataaagtaac taaagttgaa cttaaaaaag ttttcaaacg aagttttatg tatggttctt
    23041 catggaacta tgagagaatg cagaacctag gttttctata tacaattctt ccagtattga
    23101 aaaaactata cccagacaaa gattcagctt ctcctgcaat gaaacgtcac cttgagtttt
    23161 tcaatactca tcaaacagcg gcaccattta ttcttggagt tacttccgct atggaagaac
    23221 aagaaggaaa tgaaggtgca gcttcaatta ctggtattaa agttggcttg atggggccac
    23281 tggctggtct aggagatagt ttgttctggc tgacactagt tcctatctgt tttagtattg
    23341 gtgcgtctta ttctaaagac ggcggtgctt taggtatctt tatcgcctta atattgttta
    23401 atattattaa tattcctgtt aaatatttcg gtttgaaata tgggtatact aagggttcta
    23461 gtcttatcca agaaaataat acaaaaggaa cattgaatcg cgttacgagt atggcgacag
    23521 cattagggct agtactagtg ggtggtttga ttccatcaat ggttggtatt aattttggat
    23581 tagaatttaa gcagggggaa cttgttattt ctgttcaaga aatgattaca aaattaattc
    23641 caggatttat ccctatggct ttgactttat taatgtgtaa attaattaga aaaggaaaga
    23701 atccggttgt actaatcttt agtgttatgg ctattggagt tatcctagtt gttttaggaa
    23761 ttttgaagta gtagaaagtg tggaggtggt atttgggata tcacctccat tttggaagag
    23821 aggtaaagag tgaaattatg gtataagaaa gctgcctcaa attggaatga agccttgccg
    23881 attgggaacg gtcatttagg tggtatgatt tatggttcag ctacaaaaga atgtattcaa
    23941 ctaaacgatg agactatttg gtatagagga aagtcagata gaaataatcc agactcacta
    24001 ttgcatctta aaaaaattcg ggaatatctt ttagatggag aaattcagaa agccgaagaa
    24061 ttgataaagt taacaatgtt tgctacccca agagatcaaa gccactatga attacttggg
    24121 gaactttaca ttgagcatat agatattcag tcttgtgctc tttcattgta tgaaagagag
    24181 ctagatttag atacagctat ttctaa
//
LOCUS       DAVMEF010000012        23667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMEF010000012 DAVMEF010000000
VERSION     DAVMEF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.012"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..1341
                     /locus_tag="VYQ30_000343"
     CDS             70..1341
                     /locus_tag="VYQ30_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1965515.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1452..2744
                     /locus_tag="VYQ30_000344"
     CDS             1452..2744
                     /locus_tag="VYQ30_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1965516.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2746..3588
                     /locus_tag="VYQ30_000345"
     CDS             2746..3588
                     /locus_tag="VYQ30_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1965517.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3841..4641
                     /locus_tag="VYQ30_000346"
     CDS             3841..4641
                     /locus_tag="VYQ30_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1965518.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTVSQASSIVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4651..5637
                     /locus_tag="VYQ30_000347"
     CDS             4651..5637
                     /locus_tag="VYQ30_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1965519.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5773..5853
                     /locus_tag="VYQ30_000348"
                     /pseudo
     CDS             5773..5853
                     /locus_tag="VYQ30_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6063..7004)
                     /locus_tag="VYQ30_000349"
     CDS             complement(6063..7004)
                     /locus_tag="VYQ30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1965520.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLTFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(6982..8745)
                     /gene="aspS"
                     /locus_tag="VYQ30_000350"
     CDS             complement(6982..8745)
                     /gene="aspS"
                     /locus_tag="VYQ30_000350"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1965521.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKEAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETVHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(8864..8947)
                     /locus_tag="VYQ30_000351"
                     /pseudo
     CDS             complement(8864..8947)
                     /locus_tag="VYQ30_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(8981..9061)
                     /locus_tag="VYQ30_000352"
                     /pseudo
     CDS             complement(8981..9061)
                     /locus_tag="VYQ30_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9097..9327)
                     /locus_tag="VYQ30_000353"
     CDS             complement(9097..9327)
                     /locus_tag="VYQ30_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965522.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(9345..9986)
                     /locus_tag="VYQ30_000354"
     CDS             complement(9345..9986)
                     /locus_tag="VYQ30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1965523.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(9998..10720)
                     /locus_tag="VYQ30_000355"
     CDS             complement(9998..10720)
                     /locus_tag="VYQ30_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1965524.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(10723..10920)
                     /locus_tag="VYQ30_000356"
     CDS             complement(10723..10920)
                     /locus_tag="VYQ30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965525.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(10920..11126)
                     /locus_tag="VYQ30_000357"
     CDS             complement(10920..11126)
                     /locus_tag="VYQ30_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1965526.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(11136..11450)
                     /locus_tag="VYQ30_000358"
     CDS             complement(11136..11450)
                     /locus_tag="VYQ30_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965527.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(11720..11800)
                     /locus_tag="VYQ30_000359"
                     /pseudo
     CDS             complement(11720..11800)
                     /locus_tag="VYQ30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(11791..13080)
                     /locus_tag="VYQ30_000360"
     CDS             complement(11791..13080)
                     /locus_tag="VYQ30_000360"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1965528.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(13158..14405)
                     /locus_tag="VYQ30_000361"
     CDS             complement(13158..14405)
                     /locus_tag="VYQ30_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1965529.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(14598..15449)
                     /gene="rgg"
                     /locus_tag="VYQ30_000362"
     CDS             complement(14598..15449)
                     /gene="rgg"
                     /locus_tag="VYQ30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1965530.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            15573..15914
                     /locus_tag="VYQ30_000363"
     CDS             15573..15914
                     /locus_tag="VYQ30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1965531.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            15972..16052
                     /locus_tag="VYQ30_000364"
                     /pseudo
     CDS             15972..16052
                     /locus_tag="VYQ30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(16213..17916)
                     /gene="ilvD"
                     /locus_tag="VYQ30_000365"
     CDS             complement(16213..17916)
                     /gene="ilvD"
                     /locus_tag="VYQ30_000365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1965532.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(18149..19081)
                     /locus_tag="VYQ30_000366"
     CDS             complement(18149..19081)
                     /locus_tag="VYQ30_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1965533.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(19078..19935)
                     /locus_tag="VYQ30_000367"
     CDS             complement(19078..19935)
                     /locus_tag="VYQ30_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1965534.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNSGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQDLEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(19939..21285)
                     /locus_tag="VYQ30_000368"
     CDS             complement(19939..21285)
                     /locus_tag="VYQ30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1965535.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(21298..21582)
                     /locus_tag="VYQ30_000369"
     CDS             complement(21298..21582)
                     /locus_tag="VYQ30_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1965536.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(21586..23616)
                     /locus_tag="VYQ30_000370"
     CDS             complement(21586..23616)
                     /locus_tag="VYQ30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1965537.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVGLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKKYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         7353 a         5180 c         4179 g         6955 t
ORIGIN      
        1 aagctcataa aggaaaacgt ttgcgtttcc ttatcaataa aacttgctat tctttgggag
       61 gaatacacta tgtcatctaa atttatgaag agcactgcgg tgcttggaac tgctacactt
      121 gctagcttgc ttttggtagc ttgcggaagc aaaactgctg ataagcctgc tgattctggt
      181 tcatctgaag tcaaagaact cactgtatat gtagacgagg gatataagag ctatattgaa
      241 gaggttgcta aagcttatga aaaagaagct ggagtaaaag tcactcttaa aactggtgat
      301 gctctaggag gtcttgataa actttctctt gacaaccaat ctggtaatgt ccctgatgtt
      361 atgatggctc catacgaccg tgtaggtagc cttggttctg acggacaact ttcagaagtg
      421 aaattgagcg atggtgctaa aacagacgac acaactaaat ctcttgtaac agctgctaat
      481 ggtaaagttt acggtgctcc tgccgttatc gagtcacttg ttatgtacta caacaaagac
      541 ttggtgaaag atgctccaaa aacatttgct gacttggaaa accttgctaa agatagcaaa
      601 tacgcattcg ctggtgaaga tggtaaaact actgccttcc tagctgactg gacaaacttc
      661 tactatacat atggacttct tgccggtaac ggtgcttacg tctttggcca aaacggtaaa
      721 gacgctaaag acatcggtct tgcaaacgac ggttctatcg caggtatcaa ctacgctaaa
      781 tcttggtacg aaaaatggcc taaaggtatg caagatacag aaggtgctgg aaacttaatc
      841 caaactcaat tccaagaagg taaaacagct gctatcatcg acggaccttg gaaagctcaa
      901 gcctttaaag atgctaaagt aaactacgga gttgcaacta tcccaactct tccaaatgga
      961 aaagaatatg ctgcattcgg tggtggtaaa gcttgggtca ttcctcaagc cgttaagaac
     1021 cttgaagctt ctcaaaaatt tgtagacttc cttgttgcaa ctgaacaaca aaaagtatta
     1081 tatgataaga ctaacgaaat cccagctaat actgaggctc gttcatacgc tgaaggtaaa
     1141 aacgatgagt tgacaacagc tgttatcaaa cagttcaaga acactcaacc actgccaaac
     1201 atctctcaaa tgtctgcagt ttgggatcca gcgaaaaata tgctctttga tgctgtaagt
     1261 ggtcaaaaag atgctaaaac agctgctaac gatgctgtaa cattgatcaa agaaacaatc
     1321 aaacaaaaat ttggtgaata aaaaatttgt tcaagggggg tggaaatcaa atcccccttt
     1381 gaatttatca atagagacac aaataattta gctttcttat aaaaaagtag tatcctatga
     1441 aaggagttaa tatggaaaag caacaaccta gtaaagcagc cctgctgtct atcattcctg
     1501 ggttaggaca gatttacaat aaacaaaaag ccaaaggttt tatcttcctt ggtgtaacca
     1561 tcgtatttgt cctttacttc ctagcacttg caacccctga attgagcaac ctcatcactc
     1621 ttggtgacaa accaggtcgt gataattccc tctttatgct gattcgtggt gccttccatc
     1681 taatctttgt aatcgtttat gtactctttt atttctcaaa tatcaaagat gcacatacga
     1741 ttgcaaaacg cattaacaat ggaattccag ttccacgcac actcaaagac atgatcaaag
     1801 ggatttatga aaatggcttc ccttacctct tgatcattcc atcttatgtt gccatgacct
     1861 tcgcgattat cttcccagtt atcgtaacct tgatgatcgc ctttaccaac tacgacttcc
     1921 aacacttgcc accaaacaag ttgttggact gggttggttt gaccaacttt acaaacattt
     1981 ggagcttgag taccttccgt tctgcctttg gttctgttct ttcttggact atcatttggg
     2041 ctttggcagc ttctacttta caaatcgtaa ttggtatctt cacagctatc attgccaacc
     2101 aaccatttat caaaggaaaa cgtatctttg gtgttatttt ccttcttcct tgggctgtcc
     2161 cagccttcat cactatcttg acattctcaa acatgtttaa cgatagtgtc ggtgctatca
     2221 acactcaagt attgccaatc ttggctaaat tccttccttt ccttgatgga gctcttattc
     2281 cttggaaaac agacccaact tggactaaga ttgccttgat tatgatgcaa ggttggctcg
     2341 gattcccata catctacgtt ttgactttgg gtatcttgca atctattcct aacgaccttt
     2401 acgaagcagc ttatattgac ggtgccaacg cttggcaaaa attccgcaac atcactttcc
     2461 caatgatttt ggctgttgcg gcacctactt tgattagcca atacaccttc aactttaaca
     2521 acttctctat catgtacctc ttcaatggtg gaggacctgg tagtgtcgga ggtggagctg
     2581 gttcaaccga tatcttgatc tcatggatct accgtttgac aacaggtaca tctcctcaat
     2641 actcaatggc ggcagctgtt accttgatta tctctatcat tgtcatctca atctctatga
     2701 tcgcattcaa gaaactacac gcatttgata tggaggacgt ctaagatgaa taactcaatt
     2761 aaactcaaac gtagactgac tcaaagcctt acttaccttt acctgattgg tctatcaatt
     2821 gtaattatct atccactgtt gattaccatt atgtcagcct ttaaagcagg taacgtctca
     2881 gcctttaaac tagatactaa tatcgacctc aattttgata actttaaagg cctcttcact
     2941 gaaaccttgt acggtacttg gtacctcaac actttgatta tcgccttaat taccatggct
     3001 gttcaaacaa gtatcatcgt acttgctggt tatgcctaca gccgttacaa cttcttggct
     3061 cgtaaacaaa gtttggtctt cttcttgatc atccaaatgg tgccaactat ggccgctttg
     3121 acagccttct tcgttatggc gcttatgttg aacgccctta accacaactg gttcctcatc
     3181 ttcctctacg ttggtggtgg tatcccgatg aatgcttggc tcatgaaagg ctacttcgat
     3241 acagtgccaa tgtctttaga cgaatctgca aaactagacg gtgcaggaca cttccgccgc
     3301 ttctggcaaa ttgttctacc acttgttcgc ccaatggttg ccgtacaagc tctctgggcc
     3361 ttcatgggac ctttcgggga ctacatcctc tctagtttct tgcttcgtga gaaagaatac
     3421 tttactgttg ccgtaggtct ccaaaccttc gttaacaatg cgaaaaactt gaagattgcc
     3481 tacttctcag caggtgctat cctcatcgcc cttccaatct gtattctctt cttcttccta
     3541 caaaagaact ttgtttcagg acttacaagt ggtggcgaca agggataatt tatccccgcc
     3601 accctttttc attttatact cttcgaaaat ctcttcaaac cacgtcagct ttatctccaa
     3661 cctcaaagtt gtgctttgag caacctgtgg ctagtttgca ctttgatttt cattgattat
     3721 tagcaattgt cactgtaaat aatatccttg tagcaagcaa tttaccccct atacttgaaa
     3781 taaagcgcat ttctctatat aataatactc atatagaaaa caccttttag aaagatacct
     3841 atgcttccat atccattttc ctatttttca agtatttggg ggtttcgtaa gcccctgtcc
     3901 aaacgtttcg agctcaactg gtttcaactt ctctttacca gtatcttcct tatcagcttg
     3961 tctatggtac ccattgctat ccaaaacagc tcccaggaga cctatccgct agaaactttt
     4021 atcgataatg tctatgaacc tctgacagat aaggttgtcc aggatctctc tgaacatgct
     4081 acaattgtcg atggcacatt aacttatact ggaacagtta gtcaagcctc ttctattgtg
     4141 attggtccaa gtcaaatcaa ggaattacct aaggacttgc aactgcattt cgatacaaat
     4201 gagctagtca tcagcaagga aagcaaggaa ctgacccgca tctcttaccg agccattcag
     4261 actgagagtt tcaaaagcaa agacagcttg acccaagcaa tttctaaaga ctggtaccaa
     4321 caaaatcgtg tctatatcag cctcttccta gttctcggtg cgagcttcct ctttggtttg
     4381 aatttcttta tcgtctctct aggagctagt cttctccttt atatcactaa gaaatcacgc
     4441 ctcttttcat ttagaacctt taaagagtgc tatcacttta tcttgaactg tttaggattg
     4501 ccgactctaa ttacacttat tttgggatta tttggccaaa atatgacaac cctgattact
     4561 gtacaaaata ttctttttgt tctgtatctg gtcactatct tttataaaac acatttccgt
     4621 gatccaaatt accataaata ggagattttt atgcccgtta cgattaaaga cgtggccaag
     4681 gctgctggtg tttcgccttc aaccgtaacc cgtgttattc aaaataaatc aaccattagc
     4741 gacgaaacaa aaaaacgcgt tcgcaaggct atgaaggaac tcaactacca cccaaacctc
     4801 aacgctcgta gcttggtaag cagctatact caggttatcg gattggttct tcctgatgac
     4861 tcagacgcct tctaccagaa tcctttcttt ccatcggttc tacgtggcat ctctcaagtc
     4921 gcatctgaaa accactatgc cattcagata gcaacaggga aagatgagaa ggagcgtctc
     4981 aacgctattt cacaaatggt ctacggcaag cgtgtagatg ggctaatctt tctctatgcc
     5041 caagaagaag accctctcgt aaaactcgtc gcagaagaac agttcccctt ccttatctta
     5101 ggtaaatctc tatctccttt catcccactt gtcgacaacg acaatgttca agctggtttt
     5161 gatgcgactg aatatttcat caaaaaaggc tgcaaacgca ttgcctttat cggaggaagt
     5221 aaaaagctct tcgtgaccaa agaccgttta acaggctatg aacaggcgct taaacattac
     5281 aaacttacca ctgacaacaa tcgcatctac tttgccgacg agtttctgga agaaaagggc
     5341 tataaattta gcaagcgatt attcaagcac gatccacaaa ttgacgctat catcacaacc
     5401 gatagcctcc tagctgaagg tgtttgtaac tatattgcca aacaccagct ggatgtccct
     5461 gttctcagct ttgactcggt taatcccaag ctcaacttgg cagcctatgt cgatatcaat
     5521 agtttagagc ttggtcgtgt ttcccttgaa actattctcc agattattaa tgataataaa
     5581 aacaataaac aaatttgtta ccgtcaattg atcgcccaca aaattatcga aaaataagag
     5641 actgggcaaa gagtcgttaa aagcaaaaac gcatactatc aggtattgaa aaaacttgat
     5701 actatgcgtt ttattgtggg aagatttact tccttttcta ctgaaattga gtcttttccc
     5761 aagatctttt tatactcaat gaaaatcaaa gtgcaaacta ggaagctagc cgcaggttgc
     5821 tcaaaacact gttttgaggt tgtggataga actgacgaag tcagctcaaa acatggtttt
     5881 gaggttgtag atgaaactga cgaagtcagc tcaaaacatg gttttgaggt tgcagatgaa
     5941 actgacgaag tcagctcaaa acatggtttt gaggttgcag atagaactga cgaagtcagt
     6001 aacatatata cggtaaggcg acgctgacgt ggtttgaaga gattttcgaa gactattaat
     6061 cactaattat ctatctcaac aaatcttcct agaatatgaa cattttccga gacagagaca
     6121 aaggagcttg gatccacttg tgtcataatc tgtttaaatt cattaaactc tgcacgtgta
     6181 atgacagtga ttaaaactgc ctttctctcg tgattatagg ttccttctgc atcgtggatc
     6241 atggttgctc cgcggtgcaa ttttttatgg attttttcaa ttaccttctc tggatgattt
     6301 gtcacaatca tggcctgcat acgcttttgc ttagtaaaga ctgcgtctgt cacacggcta
     6361 gagacaaaga tggtaatcat agaataaaga gcgtatttcc aaccaaaggt caaacctgct
     6421 atcagcatga tagttccatt taccaagaaa gaaatactac cgacattctt acccgttttc
     6481 ttgcgaatag tcaggctgac gatatccgtc ccaccactgg agatattgtt tcgaagagca
     6541 aaaccaatcc ccaaacccat aacaacacca ccaaaaaggg cattgataat gggatcctcc
     6601 gtcaaggttg ccacagggac aaactggata aagaaggaac tcatagatac cgtgataaag
     6661 gtaaagacgg tgaacttatg gccaatctga taccaagcta agaccatcaa agggaagtta
     6721 atggcgtaga aggttagcga aatcggaata tgaaaaccaa accagtgatt actcaaggca
     6781 gagataatct gtgccagacc tgttgcacca ctcgaataca catgccctgg ttggaaaaag
     6841 aaattaactg ctactgctga taaaaaacca tagaccagag aggccgaaat cttctcatca
     6901 tacttttctc gagagatact ttgtaagaca cgtaaaattt ttatctgata agcaaagcgg
     6961 cgcagataat agcgccaccg cttaattcgt tttgtttgtt tcatcttctt ctacttgtaa
     7021 gctgagttcc tctagttgtt tgagagcgac tgttgatgga gcttgtgtca ttgggtcagt
     7081 tgccttgttg ttcttaggaa aggcaatgac ttcacggata ttttcttctc cagcaagcaa
     7141 catgacaaaa cggtcaagcc cgatagccaa accaccgtgt ggtgggaaac catagtccat
     7201 ggcttcaaga aggaaaccaa actggtcatt ggcttcttca gttgagaaac caagagcctt
     7261 gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg ctaccaccac caagctcata
     7321 accgttcaag acgatatcgt aagcaatggc acgaacctta gccaaatcac cttctaattc
     7381 gtgaacagtc tcttcctgtg gaagggtgaa aggatggtgg gcgctcatgt agcggccttc
     7441 ttcttcagac cattcaaaca tcggccagtc aaccacccaa aggaagttga acttatcatt
     7501 atcaatcaag ccaagctctt tagcaatacg tccacgaagg gcacccagtg ttgcattagc
     7561 cacttcaagc gtatccgcca caaagagaac caagtcctta tcttcaagag caagcgctgt
     7621 tgtcaattct tcttggatac cagtcaagaa cttggcaact ggtccgttta attctccatc
     7681 aaccaccttg acccaagcaa gacctttggc accatactgt ttggctactt ccgtcatctt
     7741 gtcgatgtct ttacgtgaat agttgtccgc agcttctttg accacaatcg cttttacagc
     7801 aggtgcttct gaaaagactt taaagtctac acctttgacc acttctgtca agtcctgaag
     7861 caacatgtca aaacgagtat ctggcttgtc agaaccgtaa agagccatag catcatcgta
     7921 tttcatacga gggaatggta gcgttacttc gatgcctttt gtttccttca tcacgcgcgc
     7981 gatcaagcct tctgtaatat cttggatttc ttgctcagta aggaaggacg tttccaagtc
     8041 gacctgagta aattcaggct ggcggtctcc acgcaagtcc tcgtcacgga aacatttaac
     8101 gatttggtag taacggtcaa aaccagcatt catcaagagc tgtttcgtga tttgtggact
     8161 ttgaggaaga gcgtaaaaat gccccttatt aacacgagac ggcactaaat aatcacgcgc
     8221 cccttcaggc gttgacttag aaaggaatgg tgtctccacg tcgataaact ccaactcatc
     8281 caagtagttg cggatagagt gggtcacctt ggcacgaagt ttaagatttt ccaacatttc
     8341 tggacgacga aggtcaaggt aacggtaacg caaacgtgta tcgtcatttg cctcaatgcc
     8401 atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc acaataagag ctgtcacgtt
     8461 taactcaacc gcaccagttg gcaacttatc attggcttgc tcacgcgcag cgacctgacc
     8521 agtcacctca ataacaaatt cgctacgaag gctttcagct gttgccataa cctctgcaga
     8581 tactttttca gggttgataa ccaactgcat gattccttca cggtcacgaa gatcgataaa
     8641 gatcaaacca ccaaggtcac gacgacggcc aacccatcct ttcaaggtta tttcttgtcc
     8701 gatgtgttcc tcacgaacac gaccagcata catactacgt ttcattattt ctctcctctt
     8761 ttattctgtt actattttac cataaaagcg caggctcttc atgaaaatca tcagaaaagt
     8821 ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta gcgctaatac tcttcgaaaa
     8881 tctcttcaaa ccacgtcagc gtcgccttgc cgtaggtatg gttactgact tcgtcagttt
     8941 catctacaac ctcaaaacca tgttttgagc tgacttcgtc agtttcatct acaacctcaa
     9001 aaccatgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt
     9061 ataatacaaa aatccgatga acttcaccgg actcttttat tttgaatttt tgcctgcttt
     9121 acgcttttca gcgatttcgg ctgcctttcg aggcaagaca atttccgtta tgtaagccgt
     9181 cccaaaacgc agtacacctg caataggagc aaagacaact gctagatagt tatagaagaa
     9241 atcgcctttg aaggcataag ctagcgctcc aatgatgaaa aatagaacga ctgcctgaat
     9301 cactgctaat aaaattactc gtttcatgtg acctcctgac tctattatag catgagaatc
     9361 atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa tactgtagac cagacctttt
     9421 ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa aatagacaaa aaattgttgc
     9481 acatcacctg gaaaatgaat caaggcaaat agaagactag ataccagaag aaaaatcagg
     9541 gttcgtttac tattgtcctg cttaggaaag agatagcgtg ctaacatccc tctaaaaaca
     9601 atctcttccg tcaaaggagc aaaaataacc acagcaaaga atgagaaaag tggttgagac
     9661 aaggtcaagt ctgtcgctat ttgctgattt actgaaggat catctggcaa gaagaattga
     9721 acgaccagag ataagaacca aaccaagaca ggaagccaaa taaatcgatt aaagccgctc
     9781 ttctcaatat gaacaggagc cttctgatac catttgtaaa tgccgtacac atatactcca
     9841 gccaaggcca catagagtag agtaacagca tagggtgaag cgcctaaagc aagcgacgca
     9901 gtcgcgagcc cctgaataaa gccatagata aataaaaagg atagaagggc tagaagaatc
     9961 cagccaaggt ttttaagtaa tttcatagat aactccttta tttgaaataa cgttttacca
    10021 taggtaactg catcacattg atataaacat ggatggctcc tacaagcaag aaagctagta
    10081 actgaatctc tcctgtcaag aaagaaatga taataagaaa aatatataag gctggtaaga
    10141 catattggtg taattggaat aaaattcgaa aactctgttc caaattagcc tgacgctccc
    10201 cttcatcata agaatttata tagttcaaga catcctttgg tgtagcgaaa aattctaaat
    10261 caaactgacg aacaatcgca atggttttaa aaagagattt ttgagcgact aagaatacca
    10321 caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt atgcaatata atcacctcac
    10381 ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt aatattaaaa gcaatggttc
    10441 caaactcaag attccgatac atttgcacat aataggtttc attcagatcg tcatccattt
    10501 cctcttgata caaggaatga aattttctgc ttttctttaa gaaattgaaa gtcaaaaaca
    10561 tactaatgaa acctatcagt aaacaaatag ctgatatcca tggcatcaag gcttttacat
    10621 ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat ccctacaaac atgcccaaga
    10681 caatagacat caaaaataac aatctacgtt tctttttcat attcattctc ctttttcact
    10741 tgctagattt ttggatttct tttcaatcca ttcaattact gggatgagag caaagtagac
    10801 ccaaacaaat tggtcgcttt gatagggatt aaaccagctt aggtccatcc caatcagtag
    10861 aaatacgctg actaataaag ctatgaccac tacataataa atcactttat acttgttcat
    10921 cactcgtcct cctccaaacg aaataccgat tcgactgttt cgttgaaaat ttgagatatt
    10981 ttcagggcaa tgataatgga tggggtgtac tcatcccgtt ctagtaggct aatggtctgt
    11041 ctggaaaccc ctgccagttt ggctaggtcg gtttgattga gaccatcgcg agctcgaagc
    11101 tcttttagac gattttttag ttgcacgtta cacacctact ctccgtcaaa ttcaacggtt
    11161 tggatatctt caatacgttg caacttgaat ttttcttttc ccgtattatc aacactacgt
    11221 agtttgaccc attcctcatc aacatccaca acttcccagt tatctggccc aatatacact
    11281 cccgttataa ttggttcctt tccaatcatt tcttgtaata atctcgacat ttctgcgttt
    11341 cctttctctt ttcgctcaag ttttttgatt ttattctcta gtttcttgat ttttttagaa
    11401 ttattagaat aaaagaaaat cataaatagt ataaatccta gtacccacat tataactcct
    11461 ttctgcttcc tatttcttaa cttgaattca ttgtaacata tctttttctt tttgacaatt
    11521 atagttgtca aaaagtttat gatttttgtc attttgcaaa agaaaaaggt caggagtaag
    11581 ttcctgacca ctttatctat cattaatact cttctaaaat ctcttcaaac cacgtcagct
    11641 tcaccttgcc gtaggtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaccat
    11701 gttttgagct gacttcgtca gttctatcca taacttcaaa acagtgtttt gagcaatctg
    11761 cggctagctt cctagtttgc tctttgattt ttattgagta taaaatccta gtttttcaaa
    11821 gatttctgaa aagttttggc tgattgtctc aagtgacact tgcacttctt ctcgggtttg
    11881 gttgttcttg accgtcactt gtccgctttc gacttcgctc tctcccaggg tgatgagggt
    11941 cttagtcgca aagacatcgg ctgacttgaa ctgagctttg agcttacggt tgaggtaatc
    12001 acgctctgct ttgaaacctt gttggcgaag ggcttggact agttctaggg ctttgacatt
    12061 tgctccatca cccaagactg cgatatagac atctagggca ttttcgatag ggagggccac
    12121 gccttgtttt tcaaggatga gaagcagacg ctctacacca agcccaaaac caaatccagc
    12181 agtttcaggg cctccaaagt aagcaaccaa accatcgtag cgaccacccg cacagactgt
    12241 caagtcattg ccctcaatct ctgtgataaa ctcgaaaatg gtgtggttgt agtagtccag
    12301 accacgcacc atattggtat cgatgatgta atctactcca agattttcca acatctgacg
    12361 cacagcatca aaatgagctt ggctttcttc atcaagaaag tccaagatag acggcgcatt
    12421 ctctactgcc accttgtctt ctttttcctt agagtccaag acacgaagag gattttcctc
    12481 caagcgacgt tggctatcct tagacaaggt ctccttgagc ggtgtcaaat agtcaatcaa
    12541 ggcttggcgg taggctgcac ggctctcagg atttccaaga gtgttgaggt gcaatttgac
    12601 accttgaata ccgatttcct tcaaaaaatg ggctgccata acgattgttt ccacatcggt
    12661 agctggattg ctagagccaa aacactcaac accaatctgg tggaattggc gcaagcgccc
    12721 tgcctgtgga cgctcataac ggaacatagg tcccatatag tagaacttgc ttggcttttg
    12781 cacttctggg gcgaagagtt tattttccac ataggaacgg acaacgggcg cagttccttc
    12841 tggacggagg gtaatatgac ggtcaccctt gtcataaaaa tcgtacattt ccttggttac
    12901 gatatccgtt gtatctccga cagagcggct gataacctcg taatgctcaa aaataggcgt
    12961 gcgcacttct gcatagttat agcgcttgaa aatctcacgg gcaaagccct caacgtactg
    13021 ccacttagca gactcagcag gtaaaatatc ctgcgttcct tttggttttt gtaatttcat
    13081 agggaatcct ctttaaactt aatagtctta ttttaccata aatagaggga ttaaaacagt
    13141 aagaaaaaaa ttaggattta gatatcattt ttgagattaa gaattgttaa aaaaatagct
    13201 agcaaggaaa gaccaacaaa tagcatccaa gtcaactgca tgttccatac ggctactagt
    13261 gaaaaacaag ctgttcccac aggtatggat aaggtaaaca atagacctaa aaaattacta
    13321 gtacgagcta gaacctctgg agctagattt ttcatgagca tggcactaat ctttggttga
    13381 actttaccag acacatacag agtaaagaag agaaatagca aaccaagcac gacttgattg
    13441 aataaattag ccaaaccaac tagactaagt cctacggtcg cccacatcat caatctaggc
    13501 aaggactgct tcccaaaata agcattgccc gtaaggctac tgatgatgat tgctactaaa
    13561 acacagaatt gattgataaa tagtgcctct gtataagaaa aattcaagag agaatggctc
    13621 aaaaagaaga tattataaat tccacccaaa gcgccaccca aggaattaat aagcaagaca
    13681 gcaaagagca taaaaccaaa gtttttctgt ccacttttaa gaaaaacgag acgtaaattt
    13741 cggtaaattg ttaggaactg gtctttgata gaaagcttct cattttttaa gttttcacca
    13801 tcagcagatg acattgacag gctcaatttg ctttttccta aaaagaggat agtggctgat
    13861 actaggaaaa agcaggcatt gattcccgca acgagagaaa aattgttgac cgatagagct
    13921 aagagccaga ctccgaaagc ttgaccacca atagctgaaa tataggtgat gaactgtgaa
    13981 aaagaataag cctccatcaa atcatcttca gctacttttt ccttaataag aggcatacgc
    14041 aggccacctg caaaatcact gatgatatca ctaatgacat tgatcaaaca caggctagaa
    14101 aaggcaaaga gactagcttg ctgaacaact agggctgcta gaaaaaatag aaccgcctga
    14161 aacaaaccgc tatagaccat ccatttgacc ttgtccctcg tgtaatctgc ccgaatccct
    14221 acaaaaactg taaagagggt cggaagaatc atgacaatat tcgccatagc aacagcaaaa
    14281 gatgcttgtg acaaggtcga tgcatagacg ataaagacca ggttgaaaat cgaagcacca
    14341 aaagcattga agaagcgtga taaaatcaaa attctataag ctggatttct aaacaatagt
    14401 ttcatagata atctcccttc ttgttcgaac ctctatatga agtatgacta tagtatagca
    14461 cttagaaatt tgagagggag aactcttgaa tatatgcaac aaataaaatg agcaaaaaga
    14521 aacgatagaa cttttgtaat gacctgagag agggcaatag ttctatcgct tcaaattttt
    14581 tcttggtttg cagatattca acattcggtc gtatttctat agtattcggc agatttatta
    14641 cagccaagca tctcaaaaat acggacagca tcctccatct ttttctggcc ttccttgact
    14701 ctaccttgct tgctatcaag gagaccttct gcccacagat aaacaattcg gaaataggtc
    14761 tcattttcct tgtagaaatg ctcttcgata acacgtttaa aataataggc attggtaaat
    14821 tcttcacact caatactagc taaaaagcca ttcaatagta tagtatgaaa aaggtttcga
    14881 ttgccagaca tttccattag aaaatcagat ttacgtacca tttctcgtac atatctagta
    14941 aaaagagaaa cagataaaaa tggagaactg actgaaaata aattgagttc atagattccc
    15001 cagatctcgg tagaaaacaa ataatcatga aggacttttc cttcctctgc tgttaactct
    15061 accctttcat ccatgctctt catataagac ttgataataa tggcatttag aatatgtttc
    15121 tgtttgttgt gagaatgggc atgcttttga tactccctgc gatataagtc ctcaagaggt
    15181 gctatattct ttggttccaa gacatctgta atttcttttc tcaactcaga atctgtatca
    15241 tactggaaac ctctcgccag aaagaggatc tcctccacac tggcagatat attttccaga
    15301 gcaaatagaa acttttccac cgaaagctca ctctgacctg tttcaaaacg ggacaacata
    15361 gacggcgaaa attgtcctcc ggttgcttgt ctcagtgaga tatttcttga ctctcgtaat
    15421 tgtctaaaga cttttccaat ctgctccata gacttcccct tgattccgta ttttcttcat
    15481 tttatcctat ttttcagaaa attcatcaaa aacttgccaa attgtcagaa ttatgagaaa
    15541 atagaggata tttatcacgt ggagggactg ctatgagaga cgatatcaaa atcaatgacc
    15601 gtgctttggc cttgcaagac caaattatcg aaaaactaga gaaagttttt gatacagatg
    15661 tggaattgga tgtttacaat ctaggtctga tttatgaaat caatctggat gaaacggggc
    15721 tctgcaagat tgtcatgacc ttcaccgata ctgcctgtga ttgcgccgaa agcctgccta
    15781 ttgaaattgt ggcaggtctg aaacaaatcg agggtatcaa agatatcaag gttgaagtta
    15841 cctggtcgcc tgcttggaaa atcacacgaa tcagtcgcta tggccgtatt gcccttggac
    15901 taccacctcg ttaagcaggc caatcacttt taaagatgaa aaaagcaagc cgaaaatggc
    15961 ttgctttttg atactcaatg aaaatcaaag ggcaaactag aaaactagcc gcaggttgct
    16021 caaaacactg ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa cactgttttg
    16081 aggttgtgga tagaactgac gaagtcagcc caaaacactg ttttgaggtt gtggatagaa
    16141 ctgacgaagt cagtaaccat acctacggca aggcgacgtt gacgtgattt gaagagattt
    16201 tcgagtatga gtttattttt tacctgactt gtccatattc cagaagtctg tcacggctcc
    16261 gcgtgaagca gatgatacga tgtgggcata tttaccgagg acaccacggc tgtaaagtgg
    16321 tggcaaggtt gtttctgcct tgcgtttttc aagttcttct tcggatacgg ccatggaaat
    16381 ttctttggta tcttggtcaa ccgtaacgat atcgccggta cggagatagg caattggtcc
    16441 accatcctga gcttcaggag cgatatgtcc aacaaccaga ccataagtac caccagagaa
    16501 acgtccgtcc gtcaagaggg ccaccttatc tccctgacct ttaccaacaa tcattgaaga
    16561 aagtgacagc atctcaggca taccaggacc acctttaggt ccaacaaaac gaacaacgac
    16621 tacatcgcca tcaacgattt catctgtcag aacggcctga atcgcatctt cttctgagtc
    16681 aaagacctta gctggcccaa cgtgacgacg cactttaaca cctgatacct tggcaactgc
    16741 accgtcagga gcaaggttcc cgttcaagat gataagcgga ccatccgcac gttttggatt
    16801 ttcaagtggc atgataactt tttggcctgg agtcaagtct gcaaagtcag ccaagttttc
    16861 agctacagtc ttaccagtac atgtgatgcg atctccgtga aggaaaccat ttgccaacaa
    16921 gtacttcata accgcaggga caccaccgac ttcgtagagg tcttggaaga catactgacc
    16981 agatggtttc aagtcggcca agtgaggcac acgttcttga atcgtattga agtcctcaag
    17041 tgacaagtca acatttgcgg catgggcaat ggcgagcaag tgaagagtgg cgtttgtaga
    17101 accaccgaga gccatcgtta cagtaatagc atcttcaaag gcttcacgag tcaagatatc
    17161 tgatggtttg agaccaagtt ccaacatctt aacaacagca cgtcctgctg cttcgatatc
    17221 ttctttctta tcagctgatt cagctgggtg agaggatgac cctggcaaac tcatccctag
    17281 aacttcgata gcagttgcca tggtattagc agtatacata ccaccacagc caccagggcc
    17341 agggcaggca ttacattcaa gacgtttcac gtcctcagct gtcatgtcac cgtggttcca
    17401 ttttccgata ccttcaaaga cagaaaccaa gtcgatatct ttaccatcaa gatttcccgg
    17461 tgcaatagtt ccaccatagg cgaaaatagc tgggatatcc atattagcaa tagcaatcat
    17521 agatccaggc atgttcttgt cacagccacc gatagcgaca aaggcatcca cgttgtgacc
    17581 actcatagcc gcctcgatgg agtccgcgat gatgtcacga gatgttagag agaaacgcat
    17641 accaggcgtt cccatagcga tcccgtctgc tacggtaatg gttccaaact gtacaggcca
    17701 agcgcctgca gatttgacac cttctttagc cagtttcccg aaatcatgca agtgaatgtt
    17761 acatggtgta ttttccgccc aagtcgaaat cactcccaca atcggtgttt caaagtcctt
    17821 atctgtcata ccagtcgcac gaagcatagc acggttaggt gatttaacca tgctgtcata
    17881 aatgctactg cggtgacgtt tatctaattc agtcatctta tccctcccat ttcagttttt
    17941 actattatag cacaattttc gcatgaagaa cagaataaaa atcttgaatt ttcagaaaat
    18001 tctatacaca tgtgaaatat ttaaaattaa aaacaacaaa gcggattagt gcactttctg
    18061 atgaccagaa tatgcttttt aatccgcttt ctttaaataa cgtactgtaa tttttacaga
    18121 aattctttca gataagtgta tttaacatct atcttgcatt ataaatttct agaaccttct
    18181 cttttatatt cgattcactc aaaccatact cattaagaag ataatccatt ttccctactt
    18241 gaccgaatct ttcttgaaca cccatccgat gaatttttgt cattccatca tcagagaata
    18301 attcacataa agaactgcca attccaccta tctgattgtg gttttctaca gtaaatatag
    18361 tttttccact taacattgtt tttatctgtt ctggtatcgg tttgattcta aataaatcta
    18421 tcacacttac tgaataacct aatttagaca gttcatctgc aactcgaata cttggagcaa
    18481 ccattatacc agaagcaacg attacaagat cttcaccatg ccttaactca atgtagccct
    18541 tagaaaaatc ttctccacct tgatacacag gaactggagc ttttctaatt gttcgaatat
    18601 attttagtcc ttttaagtct aatgtctggt tcaatatttc acgaaattgg atatcatcag
    18661 ttgcttcaaa aatgatcgat ttaggaatta aacgtaacaa tccaatttct tcaaatggca
    18721 tatgtgtccc accattcatt tctgcagtta ctcctgcatc tgatccaatc acagtggcat
    18781 ccaattgtgc gtatccaaga gaaataaata attgatcaaa tactcttcgt gaagcaaaag
    18841 gaccaaatgt atgaagataa ggtctaaacc cctgaataga caagcctgct gcaaggccga
    18901 ccatttctgc ttccataatc ccaacattca cataacggtc tccaaagtcc ttttcaagat
    18961 tatgagtagc catcgaactt gacaaatcgg cttctaagac tactatatca gaatcacttt
    19021 gattagcctc taaaaggaag tctctatata catgccgtaa ttctttcgta cttctcatca
    19081 ttctgtttcc tccaattcct gacttaatct ttctacaact gaagttaaca tttgtttctc
    19141 ctctacagta gggcgaagat gatgattgga tttcatttct tccagatctt gaaccccttg
    19201 acctttaata gtatctaata caatgcactt aggtgatgaa ttatttgact gttttaattg
    19261 gacaatccct tcataaattt ctctaatatc tgaacccttg accctaatgg attcaaatcc
    19321 aaatgctgaa aatttttcta cgaaatcacc tgaattacaa atatcctttg taaaaccatc
    19381 taattgtttt ttgttatcat caacaaatac aattaagttg gataactgtt gatgagaagc
    19441 aaactgtata gcctcccaac attgtccctc atttaactca ccatctccaa caatagcgta
    19501 agtataaaag ggactctttc ttattctctg accatatgca agtccagttg caacactaat
    19561 tccttgtcct aaagagcccg ttgtcatatc tatgcctggc gttagatttc tatcaggatg
    19621 agacggtaat ttggttccat ttgtatttaa agaatataag aattctttgt caaagaaacc
    19681 attcaaatag agtgtactgt atagagctgg tcctccgtga ccttttgata atatgaaata
    19741 atctctatct cgtgctgcaa atatttctgg agtcattggc attatttcac cataaagcac
    19801 cgctaaaact tctacgatag acagacttcc tccgtaatgt ccgaatccaa gatgattcaa
    19861 tgttctaaga gtatttaatc ggatgttagt cgcaaatttt cttaactcat cttctctatt
    19921 tttacttaaa atcatccctt attcctcctt tgcagatggc tttttaataa aggatactcc
    19981 aaacataact gctagaataa gaacaagacc aatcacaatg cctgcttgtg agccaaattg
    20041 atttaacatt cctaaaataa ttcctgatag accaaaatct gcatctgaga aagttgatcc
    20101 ttggaaacca agtcctccca aaactggcat taaaaagact ggaagaaaac tgattaaaat
    20161 accttgtaaa aatgctccaa tagtggctcc acgaacacca ccagatgcat tcccaatgac
    20221 acctgcagtc gctccacaga agaaatgagg cacaacacct ggtaagataa caaccgttcc
    20281 tgaagcaatc ataattgcca tacttactaa accaccaaca aaactagaga taaatccaat
    20341 tagaactgca ttgggtgcat aagtataaac aatcggacaa tccaaagcag gttttgaatt
    20401 aggtacaaga cgctctgaaa tacctttaaa ggctggaaca atttcaccca aaataaggcg
    20461 aacacctgct aaaataacaa atacccctgc tgcaaattga cctgctaatt gtaaagcata
    20521 aactagacca cttgtaccac tactgatttc tttttctata tattctgacc ctgcaaagat
    20581 agctacaata atgtaaataa ctgccatgga taaagtgata ctaacagtac tatcacgtaa
    20641 aaaagctaaa ctctttggaa atttaatgtc ctctgttgat tttgatttgt caccgataag
    20701 gctaccagta aaaccactca accaatatcc caaagaactg aaatgaccta aagctacctt
    20761 gtcatttcca gttaattgaa ccatatattt ttgcacaaat gctggggaaa tactcataat
    20821 aataccgagt gctaatcctc ctagtaagat gagaggcaag ctagtaaagc cagcaactga
    20881 taaaatgacc gcaatcatac atgccatata tagagtgtgg tgccctgtta aaaaaatata
    20941 tttaaatcga gtaaaacgag cgattaagat attgaacacc atgcctgcaa acataatcat
    21001 tgcagtagct gagccatatg ttgttaaagc tacagctaca attgcttcat tattcggcac
    21061 aacgccagat aaatgaaaag catgctcaaa catggtacca aatggattta aagaattttg
    21121 tacaattcct gcaccaccag atacaactaa gaaaccaaca aaggtcttaa ttccaccttt
    21181 aataatatca ggtaatttct tcttctgaag aactaatcct aagattgcaa ttaaagctac
    21241 taaaatagct ggtgtactaa caatatccaa tatgaacttc atcatgacgc tagcctccta
    21301 tataagtcct ttttcttcac aaagtttagt aattaattct cgtagttcat ccatatcaat
    21361 aatactattt aagatacgaa catctccaag atgactagct gaatcagcta gatcacgacc
    21421 aacaatccaa atatcagctg catttggatc tgctccacct aaatcataat gttcaacttc
    21481 tacatccgaa acattcaaat cactcaatac agattcaata ttcatctgta ccataaaact
    21541 tgaacctaat cctgaaccac aagctgtacc aatttttaac attatctaat cctcctgttt
    21601 aattatcatt ttaatgtcat catagttttt tgatgatatt aaagtttgaa catgattttt
    21661 atctcttaaa attgttgtta aatgtgacaa agcctttaaa tgactctcat tatcaatggc
    21721 tgcaatacaa atcaacaatc ttacctcttg ttctggatta tccaataaat aaatcggttc
    21781 ttccaaaact aacattgaca ttcctatttc attcacacct tcatctggcc gagcgtgagg
    21841 aattgctact cccttcccta aattaataaa aggcccaaac tcttctactt tttgaatcat
    21901 tgcctcaggg tagttctcag ttatcttatc ttgatccaaa agcggtttag ctgctaaacg
    21961 aatcgcctcc ttccatccta atttttgcga actaacctga taggtttctt tggtaataag
    22021 ttgttctagc actggtacaa tttcctttct atcatttttt tgataaagat aattctttaa
    22081 cgccaatctt aattccaatt cttgtgtaat aattccatat tttttgacaa tattcaggat
    22141 ttgttcaatc tcaaaatctc catactctaa attcggaaaa tcttttaaca ctagttctac
    22201 tagttgtatt gcttgctctt cagtcatcat aaccgaaact agataatttg gcttttctgt
    22261 ctccaccttt atggtagaaa aaaccatatc atagtcacta ctagctttca cctgtaaatc
    22321 atcaatcttt gaggttccta taaactcaat ttgaggaaat aatgctaata gattctcttt
    22381 taacatcaat gaagaactaa caccattagg acaaatgatt gctgctttat accatttttg
    22441 aggcaaagta tctgctttct ttaaataacc tccgaaatgg ataacaaaat atgctgtttc
    22501 actatcaggt atgggattgt caatagcgtc catcaagggc atcaaagaat ctttgactaa
    22561 ttcaaataaa tcaggataat gttctttaac atgcaataca tattcatttg aactaggtag
    22621 gccgaacttt aatctatagt aagccggtat aaggtggcgg cgaagatttt ctctcaatcc
    22681 ttccctttgt ttaaaatgta acaaagaaat atcttccatt ctacttataa tagcctctgt
    22741 taattgatta aagtaaaccg gagcaacatc tacttcacct tcaaagcaac ttgataataa
    22801 aacggtgata tagcgataat catcctcaga aaacaccgta tctataattc ccaaatcaac
    22861 cactgtatcc aataaaatag tggttatatc ttgaataaca ggagatacta atgtctctga
    22921 aagacctact ctttcaacat ccctttgata cctacacaga atgaatacta aaccgaaaag
    22981 gcaaactttt aattgattaa caataggtac tagctgtagc ttctcataat aatctttaac
    23041 tacctgattt atcaaatcat aagttaatga atacccccaa ctggataaaa cataatccaa
    23101 accccaaatc cctatggagg attccagcaa ctcactaacc atttgaaaag ctaaacggtg
    23161 cttattccac tctgaaccgt gtaaagtata accttttgct ctactgtacc ctagctccaa
    23221 atcattatct aacataatct ttcttaatga ttgaatatca gataaggttg tattcttact
    23281 tactttcaaa aagtcttggt aatgattatt cgatataaaa tctaatcggc aaaaagtgta
    23341 aagatagatt aaagctaagc gagtcgactt tggtaaaacc aattcatccg acttaataat
    23401 atctgtcaaa gactgcttcg tacgatttga taaactatat cgaccttgtt ttttatccag
    23461 cactatccct ttattagcta gataaggcac taaataatct attccttctt tgacttcctt
    23521 tataggtaag ctcaccttaa cagataattc atataatgat agctcacaat gatccatcaa
    23581 agtcatcaaa ataactagtg ctctataatc aaacatttct cttcctttct aactacaatt
    23641 atagtgtaaa aattataaga ataaaag
//
LOCUS       DAVMEF010000013        21988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMEF010000013 DAVMEF010000000
VERSION     DAVMEF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.013"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..120)
                     /locus_tag="VYQ30_000371"
     CDS             complement(<1..120)
                     /locus_tag="VYQ30_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEV1965538.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEA"
     gene            complement(334..1965)
                     /gene="rlmD"
                     /locus_tag="VYQ30_000372"
     CDS             complement(334..1965)
                     /gene="rlmD"
                     /locus_tag="VYQ30_000372"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1965539.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2039..3013)
                     /locus_tag="VYQ30_000373"
     CDS             complement(2039..3013)
                     /locus_tag="VYQ30_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1965540.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3087..3701)
                     /gene="pmp23"
                     /locus_tag="VYQ30_000374"
     CDS             complement(3087..3701)
                     /gene="pmp23"
                     /locus_tag="VYQ30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1965541.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3701..4675)
                     /locus_tag="VYQ30_000375"
     CDS             complement(3701..4675)
                     /locus_tag="VYQ30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1965542.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4683..5939)
                     /locus_tag="VYQ30_000376"
     CDS             complement(4683..5939)
                     /locus_tag="VYQ30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1965543.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6001..6432)
                     /locus_tag="VYQ30_000377"
     CDS             complement(6001..6432)
                     /locus_tag="VYQ30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965544.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSLEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6434..7036)
                     /locus_tag="VYQ30_000378"
     CDS             complement(6434..7036)
                     /locus_tag="VYQ30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1965545.1"
                     /translation="MIDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7020..7859)
                     /gene="prmC"
                     /locus_tag="VYQ30_000379"
     CDS             complement(7020..7859)
                     /gene="prmC"
                     /locus_tag="VYQ30_000379"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1965546.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     RQD"
     gene            complement(7859..8938)
                     /gene="prfA"
                     /locus_tag="VYQ30_000380"
     CDS             complement(7859..8938)
                     /gene="prfA"
                     /locus_tag="VYQ30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1965547.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8935..9525)
                     /locus_tag="VYQ30_000381"
     CDS             complement(8935..9525)
                     /locus_tag="VYQ30_000381"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1965548.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9649..9831
                     /locus_tag="VYQ30_000382"
     CDS             9649..9831
                     /locus_tag="VYQ30_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1965549.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAIVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            9969..11342
                     /gene="mnmE"
                     /locus_tag="VYQ30_000383"
     CDS             9969..11342
                     /gene="mnmE"
                     /locus_tag="VYQ30_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1965550.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTNSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11389..12324)
                     /gene="dapA"
                     /locus_tag="VYQ30_000384"
     CDS             complement(11389..12324)
                     /gene="dapA"
                     /locus_tag="VYQ30_000384"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1965551.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12380..13456)
                     /locus_tag="VYQ30_000385"
     CDS             complement(12380..13456)
                     /locus_tag="VYQ30_000385"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1965552.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14087..14159)
                     /locus_tag="VYQ30_000386"
     tRNA            complement(14087..14159)
                     /locus_tag="VYQ30_000386"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14124..14126),aa:Thr,seq:ggt)
     gene            complement(14259..14768)
                     /locus_tag="VYQ30_000387"
     CDS             complement(14259..14768)
                     /locus_tag="VYQ30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1965553.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14766..14862)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14891..15322
                     /locus_tag="VYQ30_000388"
     CDS             14891..15322
                     /locus_tag="VYQ30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1965554.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLLFTFLFVTQFP
                     HIIGQFVNENIDKINAIETIIAQILIIIINYILSKIYIFRK"
     gene            15404..15781
                     /gene="mscL"
                     /locus_tag="VYQ30_000389"
     CDS             15404..15781
                     /gene="mscL"
                     /locus_tag="VYQ30_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1965555.1"
                     /translation="MLKDLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            15897..17187
                     /locus_tag="VYQ30_000390"
                     /pseudo
     CDS             15897..17187
                     /locus_tag="VYQ30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(17342..18436)
                     /gene="hemH"
                     /locus_tag="VYQ30_000391"
     CDS             complement(17342..18436)
                     /gene="hemH"
                     /locus_tag="VYQ30_000391"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1965556.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            18591..19814
                     /gene="pepT"
                     /locus_tag="VYQ30_000392"
     CDS             18591..19814
                     /gene="pepT"
                     /locus_tag="VYQ30_000392"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1965557.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(19850..20479)
                     /locus_tag="VYQ30_000393"
     CDS             complement(19850..20479)
                     /locus_tag="VYQ30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965558.1"
                     /translation="MNNANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            20444..20665
                     /locus_tag="VYQ30_000394"
     CDS             20444..20665
                     /locus_tag="VYQ30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965559.1"
                     /translation="MDLFQELICIVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(20774..21343)
                     /locus_tag="VYQ30_000395"
     CDS             complement(20774..21343)
                     /locus_tag="VYQ30_000395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1965560.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGDHYHYIPKSSLNNPPSHSNTEEVGSSSSSVLSNTSLH
                     VHHEEEDGHGFDANRIISEDSEGFVIPHGDHNHYIKVQTKGYEAALKNKIPSLQSNYQ
                     PGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(21387..21947)
                     /locus_tag="VYQ30_000396"
                     /pseudo
     CDS             complement(21387..21947)
                     /locus_tag="VYQ30_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6401 a         4593 c         4130 g         6864 t
ORIGIN      
        1 tgcctctata actattatga cacgaaacca acaagcaagt tcaagcacct ccatatcttt
       61 attcaaatga ggactaagat attcacaaac tttatcataa acctctttat cttttttcat
      121 aaaaggggca tacaacttag caaatttatc ctaaaatttt ttgttgcctt tatcaatcat
      181 ttcaacactc ctttaaatat aaaaatgatc ctatcaatct ttatattgag cctattttct
      241 aattgatacc ttgcaagaaa ggaataatag ctgttcctat accaagtagg aatgtaataa
      301 atgcagaaag tcatatactg tcattctttt ctattaaatc attttgaagt gtctcaaagc
      361 atccatgatg gcttcttctt tttcaggtgt acactgtgga ataatttgtt tatccttttt
      421 agacttgttg taatgttctc gtaattctat tccacatttc ttttttatct gtgcaatata
      481 taatgtcgaa acttttaatt caaatttatt ccaaacatat tctttgattt gagcatatgt
      541 tgctttgctc tccgcacttg tcaaattcat ctcatccagc tcaatttcaa cacttatgtg
      601 cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg acgtgatgcg tttgaggaaa
      661 tagatccacc ggctggactt tcttcaattc atatcccaat tcttggtaga gtttgatatc
      721 acgcgccatg gttgcgacat tgcaggagat ataggcgatg cgatcggctc ctgtttgggc
      781 gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga ggatcaacca agataacggt
      841 tggttgaatc ccatctttaa gccaattctt catagcattt tcagctgtgt cacagacata
      901 gtgggcgttt gaaatattgt tcaactgagc atttttttta ctattctcaa ccgcttctgg
      961 aatcagttca acaccgtaga cttctttgac atgcttggcg actgataaac caatggttcc
     1021 aataccagaa taagcatcaa taaccacgtc atcttttttt aactctgcaa agtcaatggc
     1081 tgtttgatag agtttctccg ccatttcagt attgacttgg taaaaggctg ggccagcgat
     1141 ttggaagtca tttcccaaca tctggtccgt aatatagtct tgaccataaa gagtgcgcca
     1201 ctccttacca aaaatcgcat tggtattctg gtcgttgata ttttgcatga cagacacaat
     1261 ctctgggaac tgcttgataa cttgttcaat caattggtca acacgaaaaa cttttggacg
     1321 agttgccacc aaaacgacca tgatttgtcc tgaatagtga ccacgacgca ccacaagatt
     1381 ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt aaatcaaaac gacggagcag
     1441 gtctcgaaga gctactacga cttggtcaat gacaggatcc tggataaaga aatcttcaag
     1501 gggcatgagg ttgtgcgaat tcttacggaa aaatcctgtt tccaagacac cattcactcg
     1561 acgaacgggc acctgcgcct tattgcgata cttgactgga tgttccatac caagcgtttc
     1621 agcaacttct acatctgcaa ttccagcaat cttgtagaga ctgtccttga cttgcttggt
     1681 tttaaacttg agctgttctg gataagaaag gtgtcctaaa tccgcgattc ctgaacgcag
     1741 gtaagccaaa tctagatctt gattacggtg tggtgactgg acaaggtatt tttcaacttt
     1801 tccaaagcca atctttttat tgaccttgag gacacgcatg agaatttttt cactcggtaa
     1861 agcattctct acaaaaaaga ccaaaccatc taccttggca actcctgccc cttcatgggt
     1921 caaatcaaca atttcaactt ctacaatatc atttttcttt aacattcttt tctctttcta
     1981 ttggccgaca aaaaagacgg caacgttacg gttaccatcc attataccat gaaatttctt
     2041 aagaaccaaa actcttgaag aagttgacaa gggcttgcca gagggagctt aagaagttac
     2101 caccgtcctc tagcgcctta tccgcatcaa agttaaggtt gatattttta aaactgtcgc
     2161 cagcttgtga tacgatgctt tgtttaaggt catttagggt tttagtgaaa tctgcattgc
     2221 tgaggatatc actctttgag agattcaagg caaaattgat gatgatattg atctggtttc
     2281 ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc ttcaacaatc ttgcggatat
     2341 cttcttctgt cagatttccc ttgctttctt tagctttggc gagtcctgac ttgatatcag
     2401 ctagggcaac gtttaattta ttagcatcat agcctgattt gtccttgttt tcagcattga
     2461 tatctgacaa agcctttagc tcttcttgag ccaaatcttt attggcttgc ggcaccttgg
     2521 ctccattagc ttctagcgaa tagtagatcc cagccaaagc actctcacct gtaactggaa
     2581 tgggtgctgc tacggtgatt ttggcgtgtt ccacacccaa agttacggct gcatttcggt
     2641 acatatcctg agttacctta gtgatgtttt caggtgtttc aatcttgacc tcaagtggcg
     2701 atttgtcacc tagcttttga atcttggctg atgaatacaa ctgtaagcta gagtcattgg
     2761 ccacattcat gattttagaa taaacatcag gtgtcatggt cttgagttct ttggtatctg
     2821 ttgaggcatt gtagcccagt tttttaagag tttgattttt ttggtcttca gatagggaag
     2881 aacctaggac atattcaggt tggacatagg tttcatcgat aactttttga acatctgttg
     2941 ctgcatggac gctattcata gctgttactg cccacaagat cgcagcgcta gtcagaaaga
     3001 gtttctttct catagggaat ttcctccttt acttctttag agtaatatat ctatcttaaa
     3061 gaaaacttat aacaaaaaca cctggtctag ccagatgttg aaaagagagt gaaacatttg
     3121 atgatgtaaa ggttaagtcg tacctgtcta gaataataat agtttcctcc atttacatag
     3181 agttcagcac cgtgaaaaat ggaaatgggg tgaatataac tataagtctt tccagtcgta
     3241 ttaccaagca agggggcaac agtctcacga gagtactgtt tggctagagc caaggtattt
     3301 tccttgccat tttgggcgat aaaatcgata taggcaggtc caaaattata ggcttgaaca
     3361 gctgtccaga tatctacacc cttcttctgc gccagataga gattgcctgt cagagtttga
     3421 atgccttgcc gaatgctaga ggcattatca ttgatggtgt tggtggaacc acttgcagac
     3481 tcactagact gcataacatc gccttctttt ccttttgttt cagtataaat catagcaagc
     3541 acaagctctt cgtttgctgg ggtgtctttt tcactcaata tttctcgcac catgggttga
     3601 taggtcatga cttgtttgac atcttgatga acgcggtaag ctttatagcc agcaaaaagg
     3661 aagactgcta gtacaagcac tcttcgaatt cgtttaaaca ttatttactt tggatatcct
     3721 cgatattttt gattaagata gagtaggttc cattttcgtt ttggataaac tcaacagact
     3781 cggcgtcttg atagacgtta ttgggaacga tgagctcaat tccatttgat aaggagagtt
     3841 tttggttttc aaatttcttt aattggcgac tgacatcaat ttcatcaaat tgaacaggtt
     3901 ctggtacggc ttctttgact tggtcaataa agctcaaacg agccgtcaga ttgttgtcaa
     3961 aaaggtcatt agccaatttc tcaggtgaca attcattgct ttcttctagg ttgttgaaaa
     4021 tagctgattt gaccttggat tgaaattgaa aatcatctgt gttaaaagat tcagcaattc
     4081 tctgggctgt tttttccagt tccttgatag attttttagg agaaatctta ggagcgacag
     4141 caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt gtacttgatt cgtttttcaa
     4201 tcaggtgata cttgctactc tgaagattga ccaccaaggc ctcatcagct cctgttccaa
     4261 atccaggcag gttattctga gttagcttga ttggattatc aacttctcct ccgaggtggg
     4321 tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg ttctacacct tctttagaaa
     4381 attgaacaaa aatcaagtca ttggtcttga gattttcaga aatgctaaac tcctctttcc
     4441 agagattagc cagcgttact gatgtctcca acaaatcgtc tgtaatatga ttgaagaagg
     4501 gattttcttc ttcgaaaatc ccagtcttgg cttcatctga atacacatgt tcaatttttt
     4561 tacgcaggta ttcttcgatt tttggagtaa tattgagaaa cttatctgct aagaacagtt
     4621 cggtatcatc cggactgaac tggtgaataa tggctttctt aatataaatg tccataaaag
     4681 ttttagtcct cgtatagtag gaaggcatct gtcaattctt tgactgcact tctcacttct
     4741 tctaatacag cctcattttc tgaattctta agggttttaa tgatgagttc agccactttg
     4801 cgactttctt cttcaccaaa tccacgtgca gtaatggctg ctgctccgat acgaatccca
     4861 cttgtcttga atggtgacaa gctttcgtaa gggattgagt ttttatttaa ggtaatattg
     4921 acttcatcca acaagttttg agcaactttg ccgttttcta caactttagt cacatcaaca
     4981 aggaagagat ggttttcagt tccacctgaa ataatacgga aatcagggtc ttgcaagaag
     5041 acatctgcca tagccttgct gttcttaatt acattggcag catattcctt gaaggctgga
     5101 tccaaaactt ctttgaagga aactgcctta gccgccacaa catgctctaa aggaccgccc
     5161 tgaatacctg ggaaaatagc tgaattgatt tttttagcaa gttcttcgtc attggtcaaa
     5221 atcaaaccac cacgaggacc acgaagggtt ttgtgggtcg ttgttgttgt gatatgagcg
     5281 tatggaactg ggcttggatg aaggccagcc gcaaccaaac cagcgatatg ggccatgtcc
     5341 accatgagct tcgcaccgac agcatctgcg atttcacgga attttgaaaa atcgataatt
     5401 tgagaatatg ctgaagcacc agctacaatc agttttggtt ttacttcttg ggcttgtttc
     5461 aagatagcat caaagtctaa gagttccgtt tcaggatcaa cactataaga aacaaagttg
     5521 taggtttgac cagagaagct aacaggagcc ccatgagtca aatgaccacc tgatgccaaa
     5581 tccattccca taaccgtatc acctggctca atcaaggaca tgtaagccgc acagttagct
     5641 tggcttcctg aatgtggttg gacattggca aatttagcac cgaaaatttc ttttgcgcgt
     5701 tcaatagcaa gagtctctac aacgtctact acatcagttc caccataata acggcgtcct
     5761 gggtaaccct cggcatattt atttgtcaag atagaccctt gagctgccat aacagccttg
     5821 gaaactacgt tttccgaagc aattaactcg atattatttt gttggcgttc ttcttctttg
     5881 gcaatagcat tccagagatc agcatcatat gctttaaaat catctttgtc aaaaatcata
     5941 ggtcttctcc tttattgtgt gactagtcca ttagtttgat tttacaataa gaaaatcaaa
     6001 ctaaaagatg cgaataaacc gtttctgcat tttatcacaa gtatagccaa ctttttcata
     6061 aaatgcatga gcacccagac gatgattggc agaatttaag cggataaacc cataaccaca
     6121 tcttttggct tcttgttcca acccttgtag taaactttta ccgatacctt caccttgcgc
     6181 ttgaggtgaa actgctaaag ctaagatatt aaatcctgct ttggaataga gtgattcgta
     6241 aacttcagcg tggacatatc caagtaagac atgattagct gcatcctcat agccaagtag
     6301 gaaatgatgg gaatcctgag acagtctagc tagttggcta gccgtttcct ctagactaaa
     6361 agtataaccc aaagcctctt ggttgatgtc acatatcgct ttcacatctg tttcttgcaa
     6421 atctcttagc atttcaagcc tcctcaaaag aaatctctgg caaccgagca agaatatctt
     6481 ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc tccagacaaa tccacaatag
     6541 ttgaatcctg tccagttaga aaagcatcgt cttccagacc cagaacctct tggtcaaaat
     6601 ccttcagaat ttgttcaaag gttacaccac ttgcctgacc tgagatattg gcagacggcc
     6661 caatcaaggg acctgtctct cgaattaaat ccagtgtgat agggtgactg ggcatccgaa
     6721 atccaatagt tgcaaggtca gaatttaccc aatagggaac tcggtcattg gcttcgagaa
     6781 taatggtcaa gggacctggc aaaaaggtct ctacaagttt ttgtagataa gttggctgat
     6841 tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt gagattgagt gccttatctc
     6901 taggacgacg tttgagttgg taaacatggt caactgcttt ttcatctaag gccttggcaa
     6961 aaagaccata aacagtctct gtaggtagaa cgacagctcc accattttcc aactcttgtc
     7021 taatcctgtc tatcatcaac cacgaccatc ctattttgac caaattggtc cttgagtgtt
     7081 cgaactcttt tttcaggaag atgtttccta aaaagttcgg gaacactttg accttgcttg
     7141 tatccaattt caaggtaaat cttaccacca tctttgagat agtcttttgc atcttccgca
     7201 attctacagt aaatagctag gccatcctcg tctgcaaaga gagctagatg aggctccgaa
     7261 tgcaaaacat tcaagcctac ctctgactca tcttcacgag agatataggg tggattggat
     7321 acaattatat catatttttc agaaatttct gcaaaacaat cagatttttt aaaaaatata
     7381 ttaagatttt gattgctagc attctctgat gcaagctcta aggcctcttg agaaacatct
     7441 gctgctgtca ctgaccaatc tggtctattt tttgctagag cgagagcaat agctccacta
     7501 cctgttccaa tatccagaac tgagagattc gtctcaagat tttcagttag aataagctcc
     7561 actaactcct ctgtttctgg acgaggaatc aaaacccgtt catccacctt taaatgcatt
     7621 ccataaaaat ctgcctgtcc aatgatgtac tgagctggct tgtgagctgc tagttgctgg
     7681 taaatatctt ctacaaattg tttttcttcc tctgttgtca cctcctgctg gagggcaaaa
     7741 ataaagtctg taaaagatag atttttcaga ctacgataga caaaagagag gctttccgct
     7801 tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa ataattgagc taatttcatt
     7861 atttgtttaa ttcttctagt ttttgtgttt ggtcataaag caccagggca tccacaactt
     7921 cgtccaattt accagacaaa atcgtatcta gtttttggag ggtcaagccg atacggtggt
     7981 ctgtgacacg gttttgtggg aagttataag ttcggatccg ttctgaacgg tcaccagtac
     8041 cgattgtcga cttacgctca gcgtcttgtt catcctgagc aatctgagca aagtggtcag
     8101 cgacgcgtgc acggataatc ttcatagcct tctcgcggtt cttctgctgg gtacgttctt
     8161 cctgcatctc aaccttgata ttggttggca agtgaacgat acgcacggca gtcgcaacct
     8221 tattgacgtt ctgtccacca gcaccagagg cgtgatagat gtcgacacga aggtcttttg
     8281 gatcaatgtc gtattcaacc tcttcaactt ctggcataac aagaactgtc gctgtcgaag
     8341 tatgaacacg gccttggctt tctgtcacag gaacacgttg cacacggtgg gcacctgatt
     8401 catacttaag cttagagtat acagactgac ctgaaaccat agcaaccact tctttgaaac
     8461 caccgacacc attcatagaa gcctccatga cttcaaagcg ccagccttgg gcttcagcat
     8521 acttttggta catagttagc aaatctccag cgaaaagtgc cgcttcgtct ccaccagctg
     8581 ctccacggat ttcaaggatg atattcttgt catcgtttgg atcctttgga aggagcaaaa
     8641 ttttcagttt ttcttcatat tcttcttttt cagccttggc atctttgagt tcttgcttgg
     8701 ccatttcttc caagtccgca tctccacctg attccttaat catctcttcg gcatcgacga
     8761 tattttgaag gacttgttta tactcacggt aggctattac ggtgtcacga ttggaggctt
     8821 cttcttttga aagctccata aaacgcttgg tgtctgaaac gacatcaggg tcactcagca
     8881 attctcctag ttcttcataa cggtcttcta cagcttgtag ttgatcatag atgttcattt
     8941 ttcttcattc tccttattga tttcaggggc aaaataatgt ttacggcaaa ccgagatata
     9001 ggtttcatta ccaccaatct ggatctgttc tccatcgtaa acgggcagtc catcctgtgt
     9061 tcgcaacacc atggtcgcct ttttcttgca atactgacag atggtcttga tttcgtcaat
     9121 cttgtccgct aaaagcaaga gatatttgga accttcgaac aattcattgc gaaagtcatt
     9181 tttcaagcca aaagccatga cgggtatgtc taactcgtct acaacacgag ctaggtcgta
     9241 aacatggtgg cgtttgagaa actgggcttc atcgaccaaa acacagtaag gtttttctgg
     9301 taggtctcgg atatagccaa agatatccgt tgtttcctca atcgcaaggg cagggcgttt
     9361 catgccaatt cgactcgaca catagccaac accgtcacgc gtatccagag ccgaagtcat
     9421 aatgacaaca ccttttcctt gctcctcgta gttataggcc actttgagaa tctcaatcgt
     9481 cttaccagag ttcatggtcc cataacgata atacaactgt gccatgtttc ttgcttcacg
     9541 tccatttcta aatttttgct acattctagt atatcataat tttctgaagc tttaaacggc
     9601 aaaatgtggt aaaatagaag aaatcaaaaa ctagtggagg aagctattat gccatttgta
     9661 cgcatcgatt tatttgaagg acgcacgctc gagcaaaaga aagctcttgc taaggaagta
     9721 acggaagcta ttgtccgtaa tactggagcc ccccaatctg ctgtccatgt catcatcaac
     9781 gacatgccag aaggaactta cttcccacaa ggggaaatgc gtaccaaata agctagctta
     9841 agcagaattg cttaggcttt ttcaatctcc aagtaacatc cattgaagaa atagcctaaa
     9901 tttgttacaa tttgaaagga tacttggaaa aatttccaag aaaagagcta tttattaaag
     9961 gaaacattat gattacacgt gaatttgata ccatcgctgc tatctctact ccactaggtg
    10021 aaggggctat tggtattgtc cgcctgagcg gaacaaacag ttttgctatt gcgcaaaaga
    10081 ttttcaaagg aaaagacttg agtcaggttg ctagccatac acttaactac ggtcacatcg
    10141 ttgaccctct gactggtaaa gtcatggacg aggtcatgat tggggctatg aaatctccaa
    10201 aaaccttcac tcgtgaagat attatcgaga ttaacaccca cggtgggatt gcggtgacta
    10261 atgaaattct ccagctagct atccgtgaag gagctcgatt ggctgaacct ggtgaattta
    10321 ctaaacgtgc cttcctaaat ggacgtgttg acttgactca ggctgaggca gtaatggata
    10381 tcatccgtgc taagacagat aaagccatga acattgcggt caaacaacta gatggttctc
    10441 tttctgacct cattaacaat acccgtcaag aaatcctcaa tacacttgcc caagtcgaag
    10501 ttaatatcga ctatcctgag tatgatgatg ttgaggaagc gactactgct gttgtccgcg
    10561 agaaaactat ggaatttgaa caattgctaa ccaatctcct taggacagcc cgtcgcggta
    10621 aaatccttcg tgaaggaatt tccactgcca tcatcggacg tcccaacgtt gggaaatcta
    10681 gcctcctcaa caatctcttg cgtgaggaca aggctatcgt tacagatatt gctggtacca
    10741 cacgtgatgt catcgaagag tacgtcaaca tcaatggtgt acctctcaaa ttgattgata
    10801 cagccggtat tcgtgaaaca gatgacatag tggaacaaat cggtgttgag cgttcgaaaa
    10861 aagccctcaa ggaagccgac ttggttctac tagtgctaaa tgccagtgaa ccactgactg
    10921 cgcaagacag acaacttctt gaaattagcc aagataccaa tcgcattatt ctacttaata
    10981 aaaccgacct gccagaaacg attgaaactt cgaaactacc tgaagacgtt atccgtattt
    11041 cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg aatcaacaac ctcttctttg
    11101 aaaatgctgg cttggtcgag caagatgcta cttacttgtc aaacgcccgt cacatttccc
    11161 tgattgaaaa agcagttgaa agcctacaag ccgttaatca aggtcttgag ctggggatgc
    11221 cagttgattt gcttcaagtt gacttgactc gtacttggga aatcctcgga gaaatcactg
    11281 gggatgctgc tccagatgaa cttattaccc aactctttag ccaattctgt ttaggaaaat
    11341 aagaaaaatc catgatcctt cattcggtca tggattttat tgtctttatt agtaatctgg
    11401 tcttaagacc cctgttacag ttgccttagt tgcttcgtag tcgccatcta cgacaacctt
    11461 gataatgcgt ttggcatctt cttctggtgc tggaacaaga ggtagacgag tgggtccagc
    11521 ttcaaatccc atatagttaa gaattgcctt aactggagca ggacttggat aagagaagag
    11581 agcattaacc ttaggaatga atttacgctg aattgctgcg gctttcttca tatcgctttc
    11641 tgcaatggca gtaaacatct cgtgcatttc atccccattt gtatgagagg caacagaaat
    11701 aaccccatcc gccccaaggt tcatggcatg gaaagcatct ccatcctcac ctgtataaat
    11761 caagaactct tcaggcttgt gctcaatcaa gtaagccata ttagccaagc tagtacattc
    11821 tttgacaccg ataatatttg gatggtcagc caagcgaagc atggtttctg gagtcaattc
    11881 gacaactaca cgccctggaa tgttatagat aataattggt aggtcagaag catctgcaat
    11941 agccttaaag tgctgataca tcccttcttg agaaggtttg ttgtagtaag gaacaatagc
    12001 aagcccagct gcgaaaccac caaattccgc tacttctttg acaaactcaa tagagtcacg
    12061 cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt ccattgacaa ccttttgtac
    12121 agccgcaaac aactccaact cctcatcgtg ggtcaaagtt ggactctcag cagtcgttcc
    12181 tgcgagaaga attccatccg tatgatgggc caataaatgc tcaatcaagg ctggaatagc
    12241 atcaaagtta atggaaccat cctcatggaa gggggtaata aaggctgtaa tgattttaca
    12301 ttcttttaaa tcttgataag acacgaaaac acctctctat ttcaaagaag gagttcatct
    12361 gaactcctaa acgatatgac tattttaatt caaatttcaa ttcggctgtt ggacgaacca
    12421 atccacgttc atgaagagtt tcagcaatct gaactgagtt ccaagcagca cctttgagaa
    12481 ggttatctga aacaacccac atgtgaattc ctttttctgc atccaagtct ttacggatac
    12541 gaccaacaaa ggtatcacgc gaaccaactg cattgatggc ttgaggatag atttgatgag
    12601 ctacatcatc ttcaagaaca gcacctggga aggctgcgat agctgctttt acttcttcga
    12661 ttggagccac ttcttttgtt tcgatataaa cagactcaga gtgagctgac aagactggaa
    12721 tacgcacaca tgttgcagat actgcaatgc tatcatcttc cataattttc ttagtttcct
    12781 tggtcatctt catctcttcg taagtgtaat cattgtcagt gaagacatcg atttgtggaa
    12841 gagcattgaa ggcgatagga taatgtttct tgtcaccgcc tgaaggtaag atttccgcat
    12901 gcaaatcacg tggtttcact ccatcattca agacctcacg aagttcacgt tgtgtctcaa
    12961 gaattgctcc cataccagca cctgaaacgg cttgataagt tgaaacaatg atacggtcca
    13021 agccccattt ttggcgaacc ggctcaagag ccaccatcat ttggattgtt gaacagttag
    13081 ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc attgacctct ggaacaacca
    13141 aaggaacatc tggattttga cggaaataag atgtattatc cactactacc acgccagctt
    13201 ttactgcgta tggtgcatac ttagctgatg tcgaaccacc tgctgaaaag agagcaatat
    13261 caactccttc aaaagctgtt tcagtcgttt cttcaatcgt aatatcttga tctttaaatt
    13321 tcaatgactt acctgctgaa cgtgcagaag caaggtaacg aattttatcg attggaagtg
    13381 ttgattcttc caacattttt atcatctgag caccgacagc acctgtcgcg ccgactacag
    13441 caactgtata tcccataaat aacctctttc ggaattttct aaaaatttct ataatagaag
    13501 tattatacta ctttttccat gaattgcaaa gctttttcat cattttttgg aaaataaaaa
    13561 tccccaatcg ctagactagg gattaagagg catcttcatg tgatgagcag gttcacacaa
    13621 ctcatcaagg tccgctcctg cgttatgacc tccttatgct caatagtagc ccgaagacta
    13681 cctatcgact catcgcatct actattctac tagatagagt catttttgtc aacagccaaa
    13741 actctttata ctcttcgaaa atctcttcaa acttcgtcag cgtcgccttg ccgtatgtat
    13801 ggttactgac ttcgtcagtt tcatctacaa cctcaaaaac atgttttgag ctgacttcgt
    13861 cagtttcatc tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt ctatctacaa
    13921 cctcaaagca gtgctttgag caacctgcgg ctagtttcct agtttactct ttgattttca
    13981 ttgagtatta gttttattta cacaggtgca taagaaatat attggaaaaa tttggggcag
    14041 acataacaaa aacccttgta aatcaagggc ttttctgtct atttaatgct aattgccggg
    14101 attgaaccgg cgacctcatc cttaccatgg atgcgctctg ccaactgagc taaatcagct
    14161 tacttgaaaa gtatactata atcagaaaag cttgtcaagt ttccttagac attttccata
    14221 agaaaaagcc aggtaagagt cacccagctt gtcctgttct atttacttaa atcaattcga
    14281 cgaccaattt taccaataat aatcgctcca ataatcaatg aagcaaattc accgattcct
    14341 gttgtaaacc aagtaaggaa gaatggtaat tctgctacaa tatttaactc tgcagcgata
    14401 gtaaacatgg aaattgaaaa gaggattgaa aagaagaaat gatcttttcg aatcaatccg
    14461 ttaaagaggt aatccttgct atattttgca aaaagccaaa cacctaaact gaggaatact
    14521 aaggttgaac caccaccaac aaagacatct agcagtccga agctaaagaa attagcaatc
    14581 atacaaccga tggtaactcc gatgatgtac ttaggattgt aaaaagccat aaagttcatc
    14641 atttctgaaa tacgaaactg ataagcacca tagctgatgg catttagggg cggtgtgaca
    14701 gtcaaaacga cgtagatagc agcgacgatt gcaatatctg caacatcacg aatagttaat
    14761 tttttcatct tttctccttt ggcggttgcc cgcgtgtaat atgcttggtg aaagaagcta
    14821 agcaccaagg gttgattcat tcaaccttac tagtatagca caatagcctg ctttatgcta
    14881 tacttaaagc atgaaaaaac ctattcaaaa attttttaac aatgaaatct tagcctatct
    14941 cttttttggt atagtgacta ctcttgttgc tatactatca cgattagtca tttatcagct
    15001 aagtcatcag gaactccttg ctacggcctt ggcaaatatt atcggtattt tatttgcctt
    15061 tatcacaaat gatacgattg tatttaagca aaaaaagaaa aatagactta tccgtttagt
    15121 gaaattttct cttgctcgcc tttctacttt tctgttagat ttgctcttta cttttctctt
    15181 tgtgactcag tttcctcaca tcatagggca attcgtaaat gaaaacattg ataagataaa
    15241 tgctattgaa acaataattg cacaaatact cattattatc atcaattata tcttgagcaa
    15301 gatctatatt tttagaaaat aatgctccat ttatgagcct tttaattgcc ttctataatg
    15361 atttaggata caatgaaaca cgaaacattg aaaggaaaac agtatgttaa aagatcttaa
    15421 atcattcttg cttcgaggaa atgttattga ccttgctgtc ggtgttgtca ttgcctctgc
    15481 ttttggtgct atcgtcactt cgcttgtaaa cgacattatc actcctctta ttttaaatcc
    15541 cgctttgaaa gctgctaaag tcgaacgtat tgctcaactt tcttggcatg gagtcggcta
    15601 tggtaacttt ttaagtgcta ttatcaattt tatctttgtc ggtaccgccc tcttctttat
    15661 tatcaagggt attgaaaaag cacagaaact gactggcata aaggaagaaa aaactgccga
    15721 aaaaaaacca accgaattgg aagtccttca agaaataaaa gctctccttg agaaaaaata
    15781 atcgattaaa aggatttagc gcaaagctca atgtcattaa gttaagaaat tcaaatacaa
    15841 ttctgtattt gggtttcttt tttatgtgat aaactatagt taaggaggta tttcccatga
    15901 taaaacagat aaaagcccac ttgaataaga gtattcagag tatcattggc caaaaagttg
    15961 agttcgtcaa acaagatgaa caggccttta ctcgtaaaag aaggttatca ctagaaacta
    16021 tgattcgtac aattctgggc atgggaggaa aatcactatc aaaagaatta ctagatgcca
    16081 gactgacagt ttcaaattca gcccttgttc aaagacgcta tcagataaaa cctgaagctt
    16141 tctatgccct atttaaagaa tttacagcac ctattccact taatactgat tttccaatat
    16201 tcgctgcaga tgggagtgat atctgtattc ctcgaaatcc catggataca gaaacctcta
    16261 tccaaaccca aacggatgtt aaatcctata atctcataca cataaatgcc ctatacgact
    16321 tgacgactgg agtgtataga gatgtctcta ttcaagacaa acatgcacaa catgagcgct
    16381 tagctctgat tcagatgatg gaggcttctc cctttcgaga aagctcttgt tatcatggat
    16441 agaggctatg aaagttacaa tctcatggct cattttcaag aaaaaggttg gctttatatc
    16501 atccgtattc gagatggaaa acaatccatg ccttcctcct tcaacttgcc aaatacggag
    16561 tgttttgatc aaaaagtttc tctaaaatta tcacgaaagc aaaccaatca gctcaaaaaa
    16621 ctttatcgtg attttcccaa tgactatcat tttattccac acaattctat ttttgatttt
    16681 cttccagaaa caagtcgaaa acaagatcct gtaacactct atgaacttcc ttttcgtatg
    16741 gtgcgtttaa aagtggaaga aggaaaatac gaaactttag tgacgaatac agactattca
    16801 gtccaagaat taaaaaatct ctacgccagt agatggggca tagagactag ttttcgtgac
    16861 ctgaaataca gtattggctt ggtcaatttt cacgccaaaa agaaggaagg gattctccaa
    16921 gaaatctttg ctcgctttac aaattttaac ttttgtcgtt gggtaacctc gcaagttgcc
    16981 atcgacagta gtcacaaaaa acaaagatat aaagtgtgtt tctcagatgc ggcttatgcc
    17041 tgccgtttgt tttttaacgg ttccctttct tcccaccagt tgaaaaacta cctcaagaaa
    17101 cagttatcta ttattcgacc gaatcgaaaa tattcaagaa agataaaagc tcaatcggta
    17161 gttgatttca tctgtagagt aacataaatc tcaaaaaata gggtgaaaaa acctttattt
    17221 gtcatgcaaa aaatagaaga tgtgatagag aatcactttg atgatctcaa tcgcacctcc
    17281 tattcaagta gttagatcac taacttaatg acattgagcg caaagctaaa tccttttttc
    17341 tttactcttt gggtaacttg cctgcttttt caagcatttt tttgataaga taaggcatct
    17401 taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc atttgtaaat
    17461 cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca tcaaaggtct
    17521 cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact gtattgacca
    17581 aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag tttaccccaa
    17641 attcctggca taagtcataa cattctacat cattgtcaaa caagacctca atatgctcac
    17701 tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt tctctgagat
    17761 actccaaaac atctggttta atccatggaa taccaatatc actctcactc tgccaagtgt
    17821 tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta ttttcgaaaa
    17881 tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta tgggctgaaa
    17941 agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca atttcatctg
    18001 cccaatagtt taagagcgct tcttcctgat accagtcctt aatgaccaaa aattgaattt
    18061 gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa tggggctcca
    18121 aaatcaagca aatacactgt tcaattccgt ctgcttccat ttgaccgatc acatctggaa
    18181 taaagggact ggaaaactta ttagcaaagt agacactgta ttcattcccc agcctagctt
    18241 ctaccaaggc aacctcttga cgggtaattt tttgcagagg cgtgcctcca attcgtacat
    18301 aattatcata gagagtctga atctcgtggt cttggggtct caccccacga cgaatatttg
    18361 tgaaaaaatc agctacacct tcaaaggtaa tctcttctgg tgaaccgaag gtcatcatta
    18421 aaattgcttt tttcatgact gcttccttgt gatgttttta tcaagtttat tttatcatta
    18481 attcatcgat aagtaaacgc taacataggg acattctcaa tcttctgtct tttgtttttc
    18541 tcttctttct atgatacaat ggaaaaaata aattcaaaag gagttttttt atgacttatc
    18601 caaatctctt ggaccgcttc ttaacctacg ttaaggtcaa cacgcgctct gatgaacact
    18661 ctactactac tccaagtaca cagagtcagg ttgacttcgc aacaaatgtc ctaattcctg
    18721 aaatgaaacg tgttggactg caaaatgttt actatctacc gaatggtttt gctattggaa
    18781 ctttgccagc caacgatccg tctttaacac gtaagattgg ttttatatcg cacatggata
    18841 ctgctgattt taatgctgaa ggagtcaatc cacaggtaat tgaaaactac gatggtggtg
    18901 tgattgaact agggaattct ggtttcaaac tcgatccagc tgacttcaag agtcttgaaa
    18961 aatatccagg acaaacgctc atcacaacag atggaacaag cttgctaggt gctgatgaca
    19021 agtcaggaat tgctgaaatt atgacagcca ttgaatatct aactgctcat cctgaaatta
    19081 agcactgtga gattcgtgtt ggttttggtc cagatgaaga aatcggtgtt ggtgccaata
    19141 aatttgatgc agaagatttt gatgtggatt ttgcctacac tgttgatggt ggtccactag
    19201 gtgaacttca gtacgagact ttctcagccg ctggtgctga attgcatttc caaggccgta
    19261 atgtccaccc tggtactgcc aaagggcaga tggtcaatgc ccttcagcta gcaattgatt
    19321 ttcataatca acttccagaa aatgaccgac ctgagttaac tgaaggttac caaggttttt
    19381 accatctaat ggatgtgaca ggtagtgttg aggaggcgcg tgcaagctac atcattcgtg
    19441 attttgaaaa agatgccttt gaagcgcgta aagcatccat gcaatctatc gctgataaga
    19501 tgaatgaaga acttgggagc gaccgtgtca ctctcaactt gacagaccag tactacaata
    19561 tgaaagaagt cattgaaaaa gatatgactc caattaccat tgctaaagcc gttatggaag
    19621 atctaggtat cacgcctatt atcgaaccaa tccggggtgg aacagacggc tctaagattt
    19681 cctttatggg aatcccaact ccgaatatct ttgcaggtgg cgaaaatatg cacggacgtt
    19741 ttgaatacgt tagccttcag actatggaac gtgcagttga taccatcatt ggcatcgtag
    19801 cttataaagg ctaaaaagac gaggtagctc agctacttcg cctttctttt tattctactg
    19861 gtttttcttg atttccagta gttgtagaag attctgttgt ttcattttct gaagttgatt
    19921 cagcaggttt agaatctctt gtattgcttg gtttgttttc gtcgctagca gtttcaatgt
    19981 tagattctgc agttgcgttt ggttggttct cagcactggt gttatcacca tttgcttcag
    20041 catttcttgc tggacttgtt tcttcacttg cgctagcttt tgactggatt tgatgattca
    20101 aaactagaat agcttttgtc gattcaagta aagctgtttt gtctttactc ttagcagaaa
    20161 gttgatctaa taatgcatcc accttatcaa agtccgcatc agatccatta ttactttcta
    20221 aataagagtg aagcgacatg agaatatcgt agagtttttg atagagtaca agtgtctgag
    20281 gatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga gtcaagacat
    20341 cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg gcagctttta
    20401 gattttctac ttcttctgcc aaggattgtc tgattccttc ttcatggatt tgttccaaga
    20461 gttgatttgc attgttcaaa agactttcta cttcttcctt gctatctgtc gcagattatt
    20521 ggttgctatc taccatgtac tcctaaaaca ggagagttat aatccaagat tacaaggcct
    20581 tacagaaata agaaatccag ataagacaat gttcgtccaa gacgctattc gcttcgcaca
    20641 gcagcacgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct ctttttagtg
    20701 tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa actttcagta
    20761 aactgaccta aagctaactc aatctgtctt tgtagatgct tctgctatca gctagaagtt
    20821 gatctacttt tgccaagact gccttctcat caaaagttcc aggttgatag ttggattgca
    20881 gggatggaat cttgtttttc aaagccgctt catatccctt agtttgaacc ttgatgtagt
    20941 gattgtggtc gccatgagga attacaaaac cttctgaatc ttcacttata attcgattgg
    21001 catcaaaacc atgaccatct tcttcctcat ggtggacatg tagtgacgta ttacttaata
    21061 cagaactaga agaacttcct acctcttccg tgttagagtg tgatggggga ttgttaagag
    21121 atgacttagg aatatagtga tagtgatccc catgtcttac tatataagca tcacctgtat
    21181 ctctgacaat atcattaggg ttaaagacat atgtggctgc taattcacct gccgacaagt
    21241 cactctcagg aatgaaatga tagtgaccac catgtggtac tatagtaggt tgaaatagaa
    21301 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    21361 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    21421 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    21481 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    21541 acataatact ttccatctac cttaataata tatcctccct taacctaact gacgatatct
    21601 tgatctttcg gctgatagtt gggggctttc attaatagct cttcactaaa gagcgcatca
    21661 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    21721 cctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    21781 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    21841 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    21901 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    21961 ctataattat ttatatgatg tcttcatt
//
LOCUS       DAVMEF010000014        21418 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVMEF010000014 DAVMEF010000000
VERSION     DAVMEF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.014"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            123..305
                     /locus_tag="VYQ30_000397"
     CDS             123..305
                     /locus_tag="VYQ30_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1965561.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            250..1099
                     /locus_tag="VYQ30_000398"
     CDS             join(250..663,665..1099)
                     /locus_tag="VYQ30_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1965562.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDARFKRLVGVQRTTFEEML
                     AVLKTTYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQ
                     WVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1171..1533)
                     /locus_tag="VYQ30_000399"
     CDS             complement(1171..1533)
                     /locus_tag="VYQ30_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965563.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1911..3791)
                     /locus_tag="VYQ30_000400"
     CDS             complement(1911..3791)
                     /locus_tag="VYQ30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1965564.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEFYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3785..4654)
                     /locus_tag="VYQ30_000401"
     CDS             complement(3785..4654)
                     /locus_tag="VYQ30_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1965565.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLTWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4738..7383)
                     /locus_tag="VYQ30_000402"
     CDS             complement(4738..7383)
                     /locus_tag="VYQ30_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1965566.1"
                     /translation="MENIVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7474..8754)
                     /locus_tag="VYQ30_000403"
     CDS             complement(7474..8754)
                     /locus_tag="VYQ30_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1965567.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8925..11009
                     /locus_tag="VYQ30_000404"
     CDS             8925..11009
                     /locus_tag="VYQ30_000404"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1965568.1"
                     /translation="MKPLLETIDTRLGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFQPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(11049..12728)
                     /locus_tag="VYQ30_000405"
     CDS             complement(11049..12728)
                     /locus_tag="VYQ30_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1965569.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13484..14713
                     /gene="arcA"
                     /locus_tag="VYQ30_000406"
     CDS             13484..14713
                     /gene="arcA"
                     /locus_tag="VYQ30_000406"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1965570.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAICELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14771..15787
                     /gene="argF"
                     /locus_tag="VYQ30_000407"
     CDS             14771..15787
                     /gene="argF"
                     /locus_tag="VYQ30_000407"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1965571.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15952..16899
                     /gene="arcC"
                     /locus_tag="VYQ30_000408"
     CDS             15952..16899
                     /gene="arcC"
                     /locus_tag="VYQ30_000408"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1965572.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLERVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17110..18621
                     /locus_tag="VYQ30_000409"
     CDS             17110..18621
                     /locus_tag="VYQ30_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1965573.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     IIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18643..19974
                     /locus_tag="VYQ30_000410"
     CDS             18643..19974
                     /locus_tag="VYQ30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1965574.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            20069..>21418
                     /locus_tag="VYQ30_000411"
     CDS             join(20069..20510,20510..>21418)
                     /locus_tag="VYQ30_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1965575.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT         6219 a         4735 c         4407 g         6057 t
ORIGIN      
        1 agaaattaaa gtgggttaaa aaagaaatta actgagtaaa aactctcgaa attcaattcg
       61 ctatattatc actcaacttg aacaaatacc agaaccacaa aaaacagatg taaaggaaaa
      121 atatggactt aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa aacaatcttc
      181 cagagtcttt ctgggaaacc tactcagcat ttgcaaatac aaatggcggg aaaataattc
      241 tcgggataga tgaaaaaaac attgacacct accaaagagt caatagactc cctgcgaaat
      301 tatgaagcaa gtaaacaact aaccgatgca cgatttaagc gtcttgttgg tgttcagcgc
      361 accacttttg aagagatgtt agctgtgtta aaaacaactt atcaacttaa acacgcaaaa
      421 ggtggacgaa aacctaaatt aagcctagaa gaccttctta tggccactct tcaatatgtg
      481 cgagaatacc gcacttatga agaaattgcg gctgattttg gtattcacga aagcaactta
      541 atccgtcgga gccaatgggt tgaagtaact cttgttcaaa gtggtgttac gatttcaaga
      601 actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa aatcaatcgc
      661 cctaaaaaaa gaattagcga atgattctgg taaaaagaaa tgccacgcta tgaaggctca
      721 agcgattgtc acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtca
      781 tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttggc
      841 tgacagtggt tatcaagggc ccatgaagat atatcctcaa gcacaaactc cacgtaaatc
      901 cagcaaactc aagccgctaa tagctgaaga taaagcctat aaccatgcgc tatctaagga
      961 gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatat tttcaacaac
     1021 ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa
     1081 tcatgaacta ggattctagt tttgcaggaa gtctattgaa atactaagcc tacacacaga
     1141 ctgattccaa ttgtttttta gatttcaatt ttaccattct tcatagtcaa aaccatcata
     1201 atctggatat tgttctggcg acgttccaac cgaacgtacg agataagctt cttcgtcctc
     1261 tgtctcgatt tctctcagca cttggcattc aaaacgccat tcatcaccga agtcgaaaat
     1321 aaacttaaat ttttgtttga cacttaggct atccagatag acattttctg tgtaacgttc
     1381 ttcaacatcg tcactaacaa agctaagaaa gtaagaatct gcatgactcc actcaacatt
     1441 atccatgaaa aatgtgtgag catgatcatt tgcaaaatca aaactccata aaatcacatc
     1501 tgctagttcc gctaacgttt ttgtttttgg aatctatata tgtcggtagc aacctttttt
     1561 aaatttgacg gaaatgatcc aagcttctac agatgctggc ttttcagctt ctatttcttg
     1621 ttttactaat tttaagtgta gcataatacc tccaattgct catcgataga attgctctta
     1681 ggaaacattc actttctgct attttatcat aagtttgtct aactgtagca tttgaaacca
     1741 gataattctt ctgtttcacg cgaaacaccc tgcgccaagc aaggtgttct tgtttttata
     1801 ctcaatgaaa atcaaagagc aaactaggaa gctagctgca ggctgctcaa aacactgttt
     1861 tgaggttgta gatggaagct gacgtggttt gaagagattt tcgaagagta ttaagtcgta
     1921 taaatcgtcg acgctgtcgc aatggtatgc cactggttgg ctgtagttgc tgcgaaatcc
     1981 ttgtctgcgt agaagtcagt aaatggtagg atttcaactt ccagctcgtc gatgcggtca
     2041 agctgaccag ccagataaaa ctcgatacgg ctttctgctc gtttgatgcg ttgcaagagt
     2101 ccgcccatac ggatgtcaac tgtatccaaa ccaaagacct tgttttcttt cagccattgg
     2161 tgactaaaga gggcatggaa gtcttcaatt tggcttctaa gttctggtaa ttcttgtctg
     2221 gcgatttgtt gtaaactttc tttatcatcc gcttggtagg cctgacgaat gcgtcgtccc
     2281 acatctactt tgctacttaa aatagcattc aactgggcct gagtttcaaa gagataggca
     2341 tagtttccag ctttttcttt aatgttagca agcgtctcag cagcctgagc gaagtgcggt
     2401 ttgtcctgtt caggtgtcat gtgttgatca agaatcggac aaagaatatc ctgataaaaa
     2461 acatagcggt tgggattgat accgctgaga ttgcctggta ggtctggtaa gaggttggca
     2521 aggtcaatct gcataaaatc ctcaaccgtt agaccagtat tggtcttgaa gtgcgcagac
     2581 aaaccatcta ggtcattgcg atagctgagt tctgcccaga tttgcaagct tggtaggata
     2641 gagaactggg cagtttcacc accattgtct ccccaacccg ttacgatgac ttctttaatc
     2701 tgattggcac ggcaggcttt attagcctcg atagccacta gacggctaaa atggttgtga
     2761 ggtgtaaagc caatccactt ccaagctccc cctgcaaatg caaggtcatg gctaatcttg
     2821 tgatgattgc ggaaattacg gttgtatttt tcctcgctat cctgataata atcccagtaa
     2881 accagagtca cacggtcttt gagacggtct aggtagacac gagtttcctc tggaatttcc
     2941 acatcacgat cgtactggcc atccgctgac atgagtttga agaacatatc actccacatc
     3001 tggcagtgga aaccatattt gtcagcaata tccagcacgc gctccaagtg ttggcacatg
     3061 aggagactac gatccacaac accgttcaaa atcaggtagc gtcccaaacc aaccaagtgg
     3121 gcttcgtcca tcccgatatt gaccttgcga gtcttcagtt tagacaacgt ggcaaacatg
     3181 ccatcaatca ggtcataaac cttttcttcg ccaatgagaa gaatgtcctc tacatcacgg
     3241 agctcctgca cttccttgac accccatttg acaaaggccg acaagtgggc caaggtctgg
     3301 atgcatggta caaaggtcat gtcaaactgt tgagcatagg cttctatttc ctgcaactcc
     3361 tctgctgaat aagctccacg gaaatagcca aagtaaggct gcccttcaat ctggtaagtg
     3421 tcttccatgt aaagctcaaa ggttgagtag cccatgagag ccaagacctc aatcatctgc
     3481 ttggcagaag ccacattcag caccgcattt cgagaacagt caaccatgta agccaaatct
     3541 tcgtaagctg cttgttcctc aatctctact ttatcagctt ctgctagaac tgttaccaac
     3601 aaggacaagg cacgataaag ttggtgaggt ttgcggtagg tcaattgata gtgaccttcc
     3661 tcaccctgga tagagataga ggcttggtca gactgagtga cagccacttc cacatctggt
     3721 agagaaatgt gaccttttaa aacctcaata gcttgcgttt gtttgggact aagtcctgta
     3781 aatcttacca ttgcttttcc tcctgtagcc agttgacaag ggcaccgtag agattggcat
     3841 ctgcgtgata ggtgcaggcc tggataactg gtgcgaccgt gtattcttcg taggcatcga
     3901 caaagtcttc aacagccttc ttaacacctt ggataaaatc tggattttga ctgatagagc
     3961 cacccagact gatgacatct ggatcgatga gatactggat attgagcaag ccttgcgtca
     4021 gattgcggtt catgcgctca atggcttctt gacaaagagc attaccagct gcggcctctt
     4081 ggtaaatctt gcgaccgtcc caatcagtat gaccagattt ttcaatcacg tatcgtacca
     4141 tattcccagt tgatgctagt tgcgaccagt tattaagttt ttcagcaggg gcaagggttg
     4201 tcatgtagcc aaattctcca cccagaccgt ggcgacctcg atgaagtcta ccattgataa
     4261 tcatggctcc gccaatccct gtcccaatca cgacacaggc tgcattttca agctctggat
     4321 gagctagtag ttcactgagt ccaacgcagt tggcatcatt ttctaaatgg acaggtagct
     4381 gataagagct aagcgcctca taccaagaaa agccatggat atagggcacc gcactgaagc
     4441 catcaatcac acctgtctct tgattgaccg cacctggaac gctcatagca atcccactgt
     4501 aatcctgttc tgacaagcgt tgatctagcc acgttagtaa atcctccaag ttttcaggcg
     4561 ttgaaatact tgtcttatcc agtattttcc catcaggagt cagactggca aacttaatcc
     4621 cagtccctcc gatatcaatc gttgcaatgg tcattgtttt taccataaaa aggggcgaat
     4681 cagtagttag ttcttacctt ttcgcccctc ctttcttttg atgtttactt tttgaaatta
     4741 aatttcttct tttttgatga attctgtacg aatctcttgt ggagccaata gtcctgaatg
     4801 aactggatat ggtcgctcaa gtaagtcaag aatggtttgt tgatgttctg atatgcgcac
     4861 attttcttga ctcatattat agtaacggag gacatagcct tcttcatttt ccgccacttt
     4921 aaaggctgtt ggacaaactt gcggtatgct gagaacagaa tggctcaaga ggctaccagt
     4981 cgcaaccacg cttccttcct gtctagcaag ctgaaggctg gtaaacggtg tctgcaaggc
     5041 tttggcacga cgataggctg agaagcgttc ttgggcttgg tggcattcaa gtgcaaactc
     5101 gacttcaaac tcccgcaagc attgtgcttc tggcgttggg aagtagcccc agtcacctag
     5161 ctcacctgat gcacgcaaaa tggtcacggc aatggtgtta tccccaagga tttcgtattc
     5221 attcaatccc ttgttggata cagtcacccc tttttcatcg tcatacagac tgacaaaagc
     5281 ttgttggtgt tgaggatttt caggattttc ccatgaagca gctggtttgt ttggtcgtgt
     5341 caccacctca tagatacttt cagaatcatt gcttggacgc gtgttatgag tcttgaccaa
     5401 gagacggata cggtgatcct tggcagtgtt agtaaagcga gtcttgaagc ggatttgtgg
     5461 attgtcaacg aagacagtca actcagtttc cagaggaatg tttgtcaatt cttctgaccg
     5521 tccagcctca cgcttcataa actcgatgat accttgttgc tcttcttcta gcttttcatc
     5581 cgcgctgaca ggcacggtca attcatgttt gagcaagatt ttagcatagc aagctgtgtt
     5641 ttccaagacc tcgtggccct taagctctgc aaagattggc tctgttcctt ttggttggaa
     5701 atagatatac tcgtttccga tgtccccacg gtcttcaaag cggataaagt cttcataggc
     5761 ttcgtgagtt gtcttatcat agactgtgat gttgtcatcc atactcaccg ttacgaatgg
     5821 tgtatcaatc actccgtttt ggtaaatacc ctcacggtgt tcttgttctc cttccagcaa
     5881 ttggaaggtt gtccaagaaa gcggtgctag gtgaactgga atggtcacgc gcacttgtcg
     5941 agcaatacga gcttggcgga acttgtcttt tggtaaatcg tattcaaaat tagctccgag
     6001 gtcttcgatt gtagcctcta caggacgacc atccaagtcc tccacacggt aacttggcaa
     6061 gataagagca gccatctttt tgtagccttc tgttgggtgc aattccttga aatcacaagt
     6121 cgccacatca atcactgtgc tgacagtatc gaccttatca tgcaagcctg tgttaatgac
     6181 agtaaagaga tagtcacttt gagccttatc cgtagcaatt ttacccttcc actcgttgag
     6241 caaattactt ttaacaaagt ttcctacttg gttgaccttg gcaaaacgcg tttccatctc
     6301 gcggtgaact tcgtccacgc tacagccaca gatactatca tgtggcgcat tctgcaaaag
     6361 tgttttccaa gcataggtca actggtcctt gtggttgtgt ccaccagtga taatagtcaa
     6421 gggttctacc acttgctcta ggaggttgct attttcttgg aaggcttgtt ttaggtaaat
     6481 gcgggatgaa gaagtgttgg caagtgtgta ccagccatct gtttcctgac tggtcaactc
     6541 acctgtaaca gttgataagt gttcaggaag cgcaccttct acagcttgaa catattcatc
     6601 aaaggaacta tgaacaaaga ttacatccgg gaagagttca tttgccacac gaatggcttc
     6661 gctcagattt ttctgtacag gctggtggtc acagccgttc atcatcaacc attggttggt
     6721 cgaagcgtag gcacgcacat ctgacaattt ttgtttccag aaggtcaagg cctcatcttt
     6781 gtcaactgga atttcattcc cgttactgta ccagttggca aagagaatac ctaaaacacg
     6841 actaccatcc acaccctgcc agtacatttc tgaaaactgg gatgtaaact gctcatcttc
     6901 aaggacttgg ttgtcaaatc caatcggctt cacaccacga ccaaaggtcg ccacgtgaat
     6961 gcctgatttt tgaagaattt gaggcgcttg tcccatattt ccaaaggtat ctggaaagta
     7021 gccaatctgg gttgatttac cccatttggc agcttcttgt tgaccaatca aggtattgcg
     7081 gacattggct tcactggaga tcaaataatc atcctgcaag atgtaaaagg gaccaatttt
     7141 aagcttgccc tcgtcaatgt agcgttggac cttgtcacga ttttcagggc gaatttgtaa
     7201 gtagtcgtca aggacaatag tttgtccatc caagtggaaa ctcttgaact cagggtcatt
     7261 ttcaaagaga tcaaagagat tgtcaaacaa ttccaccaac tgcatacgat ggctttcaaa
     7321 aggcaagtac cactcacggt cccagtgact atgtgagata atatgtacaa caatattttc
     7381 catgaagtaa aacctcattc taaatttaaa ttttaacgtt ttaaatgtaa acttgtgatt
     7441 ctgataagaa tcggttgagc taaagcgagc cccttagcga atatccaagt aatctaagac
     7501 caactcacag aacatcatgt tagcccaaga gaaccattca cgagagtaga gggtcggatc
     7561 atctacgtgg aagctttcgt gcatgacacc tgtaccacca tcgcaggcaa ccagctgatc
     7621 cagcaagaat tttttctctg ccttatctct tgttgtcaat ccttggatag aaagggcgat
     7681 tggccagata tagcgataga aggtatgaga acttcctaga ccgctagcgt attctccttg
     7741 gtagaaatat ggattttcag agctcaaaat ggtacgacga gtagcttgat acacttcatc
     7801 atcgaccgaa cagtagccca gatagggcgc agctagtaga cttggtacat ttggatcatc
     7861 catgatgctg gcatttccta ggccatccac ttcaaaagcg taaatctttt cgcccttgct
     7921 gttggtggtg taagcgtagt ttttgattcc ttcttggatt tcatcctgaa gacgcttggc
     7981 atcagcaata acactctggc tatcagctag gtttaatgct gcgaagattt cttgcacata
     8041 acccaagact actacagcaa acatatttga cggaatcaag taactatact gacagcagtc
     8101 atcgctcgga cgaaaggctg accaagtcat acctgtcact gcaaagtcag gtccaaagcc
     8161 atcatttacc aaggtgtctt ccttacggtc cgtatctcgg acaaaacgat aaggagagtt
     8221 cttgtggtct tgttccaccg tccacagatg gagaatttcc ttagtcgctg cgacaaaagt
     8281 ctcatcaaac tgaccagtct cgccagtctc tttccagagg agataagcca actgcaaagg
     8341 atagcaaagc gaatccacct catacttgcg ctcccagatc cagccgttaa ggtctgtgtg
     8401 gtcagtctcg tggtgccctt tccagttctc ctcaatgttg aaggagttag catagggatc
     8461 cttgagtacc aaggtcatct gacgtttgac caaacctgca atggtctgac gcaggagggc
     8521 atctctttta gctacatgaa ggtagggtct gagttgggct gtcgaatctc gaagccacat
     8581 ggcaggaata tccccagtca agacaaaagt tgaaccatct tctaagattt caaccgtatt
     8641 gtccaaggta tcggtgtagc aacgctcgaa aacatccacc cactctggat ggtccttagc
     8701 ccgctctgct acttcatcta gccattctct aacaatttct ttcgaataaa tcatcgtgca
     8761 attccctctt tcaaacaagt ttcatgttca gtatatagct tttgagacag aaaatacata
     8821 cacaaatgat agtcattttt ctacaaaata gttatctttg aaactccttt tctaaaggcc
     8881 ctctttttct gatataatgt agaaaaacag ctaaaggaaa gactatgaaa ccactacttg
     8941 aaaccatcga tacccgcttg ggaactacca acaagcatgc cttttctcgg ggaaataccc
     9001 tgccatacac aggcgtgcct tttgggatga attactttgt gccccagacc agtgaccagg
     9061 atggatcttg gttcttcgat ccgcatctgc ctatctttca ggggattcga ttaactcacc
     9121 agcccagtcc ttggattggg gactactctt ggctccttct gacacctgtc accagtcaac
     9181 tgggtggaga cagcctcttt caccgccaat cttcctatga tagggataag gcctctttcc
     9241 aacctcatta tctaaagatt ttctccctac gctatcagat tgaaacccag ctcacaccga
     9301 cttgctatgg tgcttctatt cgtttgaagc aaaagcaagg caaagccctc tccctctatc
     9361 ttcacgcagc agatgaacta acagttgagc aggtagataa gcggactctg gctctcagac
     9421 aagaagggaa aacagagacc aataaaaatc cactgatgct cttcactgcc ctgcaaatga
     9481 acacggatat tcttgctatc agccaagaag ctggagactg gcgaattgac ttagcaagta
     9541 gtcaaaccga gatgcaacta gccacttctt tcatctctcc ttctcaagct ctaatcaatc
     9601 tacctcaaga agattttgat agctgtaaaa caagtgccca agcggattgg gaaaatctcc
     9661 tccatcgttt tgacattata gagacaggag aggctgaccg aaccttcttt gaccactgcc
     9721 tctacagact cttcctcttc ccacagactt tttatgaggt tgatgagtca gggcaagcca
     9781 tccacatgga tctggctact ggtactgtca agcctggtgt cctctttagc aacaatggtt
     9841 tctgggatac cttccgcacc accttccccc tctttgccct tatcataccg gagcactatc
     9901 aacgcttttt agaaggtttc ctcaatagct accgcgatac tggtttcctt ccaaaatggc
     9961 tggctccaga tgaacggggt atgatgccag gtacactttt agacggtatt atcgcagata
    10021 gcgcctgcaa ggacatggcc cctgacctag aaggagaact cttccaagcc atgctcgaaa
    10081 cagccagcaa agccgaccct ctcggaatca atggccgcca cggactagcc caataccaag
    10141 aactaggtta cctctctacc gaccaccacg aaagtgttag ccatactcta gactatgcct
    10201 atagtgactt ttgtatcgct agctgtgcca aaaaactaga gaacatagaa atcgctgaaa
    10261 cctacaaggc tgcttcacaa aattaccgcc agctatttga cgctgagaca ggttacatgc
    10321 gagcacgaga caatcaagga aactttcacc ctgacttctc tccttatagt tgggggcgag
    10381 actacgctga atgctctgcc attcaagcta ctttaggcgt tctccacgac atccctggct
    10441 taatccaact tatgggtgga aaagaaacct ttagcaacta tcttttgaaa gcttgtcaag
    10501 atgctcccct ctttgaaaca acaggctatg gttatgaaat tcacgaaatg agcgagatgg
    10561 ctactgctcc ttttggacaa atagccattt ccaaccagcc tagtttccac atcccttatc
    10621 tcttccgcta cagcgattac cctgactaca ctgcccttct tatcaagacg ctccgtcaga
    10681 aagcttttca gccaagttgg gaagcctatc ctggagatga agacaatggt agtctctctg
    10741 cttggtacat ctggtcagct ctcggatttt atccaacctg tccaggaaaa ccaagctatg
    10801 acctcggaat ccctctcttt gaccatctac gagtctactt agctaaagaa gataaatggc
    10861 tggatatcca tactaaacaa aaccacaacc attttaactt tgtcaaagaa tgccgactgg
    10921 acaaaacact cgtatcaact attcaacacc aagacctcct aaaagctgaa caactaactt
    10981 tcaccctcag ctggctacca aatcactaat aaccaaaaga acctttgcat cgcgcaaagg
    11041 ttcttctttt atgaaacttg gacttgaagt tgatccacaa gctgaccatc caccgttagg
    11101 ttcagataga aatccaaggt tttatctcct gcgaaataca aggttggtag cgtcagcctt
    11161 ttttcagtct caccagcttg aaacgcaagg acataaatct catcctgata ggcgacacca
    11221 tggacacctg tccccggttg aatcgaaatc ttagcttcta aaggaccact acattctctg
    11281 cgcttaactg taaaataggc attttctccc tttgccacag ctaggctttt ttctgcaaat
    11341 gctagttcct gaacaacttc tttttttgat aatccaggag ttttataaag ggaaatcttg
    11401 gtcaatacag gaatagattg tgcctcggta atcatcacgc gcaccttctg tgcttcaaca
    11461 actgctcctc gtaagagacg tttgtaacca acagtatgac ccgaaccaaa ctcctgccag
    11521 acaccatcca cctctacttg cacatgaaaa gcagcgattc gttgccctag cttcaaatct
    11581 tctcttaact caattacatc aaaagtttta ggagaaccta agtcgagttc taactggatg
    11641 ggcaagtctg catcgtttgc ccaagagctg gtctcaaggc cgtctgtcaa atggcgacaa
    11701 gcaaagtctg cggaaagagc tggaccagat acctcagctc ccagagccaa atcttcttta
    11761 tagagctcat tgcgataggt cgcaaattca taaagtcgtt caatatcctt tgcatcaaag
    11821 agcccagctt gattcggcgg aatattaagc aagagtggag ttcctcgccc tactgagtga
    11881 aagtagattt cgaccaactc ctcgagagac ttaggatcct gatcctcatg gtagaaccag
    11941 cctggacgga tggaaacatc tgcctctccg attgaaaaaa tcgtgcccga gggatccccg
    12001 tgctgaagat agttcagctc tgcttctgtt cctagtttat caggattcac cttttgccac
    12061 agtggatcac ctgcataccc tcgttcattg ccaatccagc ggatactggt gccttctgtt
    12121 gaaaaaatca agcaagcgcc ctgcaggtca cgaatggttt caaaccattt ttcaaattca
    12181 taattaacct tttgtgcgcc ctctcctctg gcaccatcca tccaaacctc agcgaactta
    12241 ccagcattcc catagttagg atttgataag atttccttca actgagccag ataataggca
    12301 ttgtagtccg cttctcggtc cacatgatag aggggactat gggcatccca cggtgacagg
    12361 tagaccccca tatccatatc aaactctgtg gcagcttggg atacttcaag gagcaagtcg
    12421 ccctttcctc tcctccaagg actgacctta accgaataat ctgtgtgagc tgtcggataa
    12481 aggacaaagc catcgtggtg cttgaccacc aaaatcaact ttttgaagcc cgtttccttg
    12541 agcacacgaa cccactcacg cgcatccaac tgactcgggt taaagcgctc aggatcctcc
    12601 tgtccagtcc cccattcttg gtcataaaag gtattaggac cgaagtggat aaaagctgct
    12661 agttcatctc ccagataagc tagctgagtc tgacttggta acggtccatg cggtttgatt
    12721 ttcttcatcg ttatatatct ccttctttag caacttctct tgttcacaag gttatccaca
    12781 ggttgtggat aataaaccat gatctaacag ccagctcttt ccaactccaa ctggtaggct
    12841 caggattttt ctcctcaata ttccctcttc aaacagctct gtcatatggt tctgctaaca
    12901 cctctcagaa tgctactctc cataattatt ctcaggcttc tttcaggtca ttgtgtgaag
    12961 accattcact ctccagactt ttccacagat tgtgaataag tgattattct aaactatcac
    13021 ctcgtcgact ataacaaaaa ataaaaagag ggtcaataag ataattatct cttcctaaat
    13081 actcagaaaa gattctataa tttgtacaat tgaacaagaa atgcttctcc aaaatctatc
    13141 ttcaaaagaa tgttgcatct ttttatcctt tcacacaaac atttctcttt atacagatcc
    13201 aagtgataac cagatgataa ttggacactt tttgtttatt tgtatatttt ttgaaaaatt
    13261 ttttaaaaaa aattttatat cctttttata gaagtttatg catttgtatc tatgtattct
    13321 atcctatgtt atgtataata acataaaatt gtgctaaaca tcataattta tattattttc
    13381 cgagaaatta tagccattga cattacctag gaattgcgtt ataacataca tgtaagcggt
    13441 acccaaaatg gtgccaagtc aaaattttta aggaggaaaa tacatgtctt cacatccaat
    13501 tcaggtcttc tcagaaattg ggaaactgaa aaaagttatg ttgcaccgtc caggcaagga
    13561 gttagaaaac ttgttgccgg actatcttga aaggcttctt tttgatgata ttcctttctt
    13621 ggaagatgct caaaaagaac atgatgcatt tgcccaagct cttcgcgatg aaggaattga
    13681 ggttctctac ctagaacaac tcgctgctga atcattgacc tctccagaaa tccgcgatca
    13741 atttatcgag gaatacttag acgaagccaa catccgtgat cgtcaaacca aggttgctat
    13801 ttgtgaatta cttcacggca tcaaggacaa ccaagaattg gttgaaaaaa caatggctgg
    13861 gattcaaaaa gttgaattgc cagaaattcc tgacgaagct aaagatctaa ctgacttagt
    13921 tgaatccgat tatccatttg caattgaccc gatgccaaac ctctatttca ctcgcgaccc
    13981 atttgcaaca attggaaacg ccgtatcgct taaccacatg tttgcagaca ctcgtaaccg
    14041 tgaaacactc tacggtaagt atatcttcaa ataccaccca atctatggcg gaaaagtgga
    14101 tttggtctac aaccgtgaag aagatacgcg tatcgaaggt ggagacgagc tagttctttc
    14161 taaagacgtc cttgcagtag gtatctctca acgtacagac gcagcttcta tcgaaaaact
    14221 tttggtcaac atcttcaaga aaaatgttgg cttcaagaaa gttttggcct ttgaatttgc
    14281 taacaaccgt aaattcatgc acttggatac tgtcttcact atggtagact atgacaagtt
    14341 cactattcac ccagaaatcg aaggcgacct tcacgtttac tcagttactt acgaaaacga
    14401 aaaacttaaa atcgttgaag agaaaggtga cttagctgaa cttcttgctc aaaaccttgg
    14461 tgtagaaaaa gttcatttga ttcgttgcgg tggtggcaat atcgtagcag ctgcgcgtga
    14521 acaatggaac gacggttcta acactttgac catcgcacct ggtgtggtag ttgtttatga
    14581 ccgcaatacc gtgaccaata agattttgga agaatacggg cttcgcttga ttaagattcg
    14641 cggaagtgaa ttggttcggg gccgtggtgg acctcgttgt atgtctatgc catttgaacg
    14701 tgaagaagtg taatcgctgt tcggtattcg tcaatagaaa atgtaaaaaa tagaaagagg
    14761 aaataataaa atgacaaatt cagtattcca aggacgcagc ttcttagcag aaaaagactt
    14821 tacccgtgca gagttagaat accttattgg tctttcagct cacttgaaag atttgaaaaa
    14881 acgcaatatt caacaccact accttgctgg caagaatatc gctctcctat ttgaaaaaac
    14941 atctactcgt actcgtgcag cctttacaac tgcggctatc gaccttggtg ctcacccaga
    15001 atacctcgga gcaaatgata ttcagttggg taaaaaagaa tctactgaag atactgctaa
    15061 agtattggga cgtatgtttg acgggattga attccgcgga ttcagccaac gtatggttga
    15121 agaattggca gaattctcag gcgttccagt atggaacggt ctaactgacg aatggcaccc
    15181 aactcaaatg ctcgctgact acttgactgt tcaagaaaac ttcggtcgct tggaaggctt
    15241 gacattggta tactgtggtg atggacgtaa caacgttgcc aacagcttgc tcgtaacagg
    15301 tgctatcctt ggtgtcaatg ttcacatctt ctcaccaaaa gaactcttcc cagaaaaaga
    15361 aatcgttgaa ttggcagaag gatttgctaa agaaagtggc gcacatgttc tcatcactga
    15421 agatgctgat gaagcagtta aagatgcaga cgttctttac acagacgttt gggtatcaat
    15481 gggtgaagaa gacaaattcg cagaacgtgt agctcttctt aaaccttacc aagtcaatat
    15541 ggacttagtt aaaaaagcag gcaatgaaaa cttgatcttc ctacactgct tgccagcatt
    15601 ccacgatact cacactgttt atggtaaaga cgttgctgaa aaatttggtg tagaagaaat
    15661 ggaagtaaca gacgaagtct tccgcagcaa gtacgctcgc cacttcgatc aagcagaaaa
    15721 ccgtatgcac actatcaaag ctgttatggc tgctacactt ggtaaccttt atattcctaa
    15781 agtataattt tagataataa accgtctacc aacagctatg ggggctgcga ctaatagctt
    15841 tagtccggtc ctcttttata taatggtaat ctattatttc ttataaaata tgtgaaaaat
    15901 cattaaattg aaatctaaac gcattctatt gagtgtgata aaggagaatt tatggcaaat
    15961 cgtaaaattg tagtagcttt gggaggaaat gcgattcttt cttctgaccc atcagcaaaa
    16021 gctcaacaag aagctttagt tgaaacagct aagcatcttg taaaattgat taaaaatgga
    16081 gatgatttga ttatcactca cggtaatgga cctcaagttg ggaatctctt gctccaacat
    16141 ttggcatcag actctgaaaa gaaccctgcc ttcccactcg actcacttgt cgctatgaca
    16201 gaaggtagca tcggtttctg gttgaaaaat gctttgcaaa atgctctctt agatgaaggc
    16261 atcgaaaaaa atgttgcctc tgttgtaacg caagttgtcg tagataaaaa tgatccagct
    16321 tttgttaact tgagtaaacc aatcggtcct ttctattcag aagaagaagc aaaagcagaa
    16381 gccgaaaaga gcggagcgac tttcaaggaa gatgctggcc gtggctggcg taaggtcgtt
    16441 gcctcaccaa aacctgttga catcaaagaa attgaaacca tccgtactct tttaaataat
    16501 ggtcaagtcg tcgtagctgc aggtggtggc ggtattcccg tcgtcaaaga aaacaatgga
    16561 catttgactg gtgtcgaagc ggttattgat aaagacttcg cttcccaacg tttggcagaa
    16621 ttggttgatg cagacctctt catcattttg acaggtgtag attatgtatt tgttaactac
    16681 aacaagccaa accaggaaaa attggaacgt gtgaatgttg cccagctgga agaatatatc
    16741 aaacaagatc agtttgcacc aggtagcatg cttccaaaag tagaagcagc tatcgctttt
    16801 gtcaatggtc gtccagaagg aaaagcagtt attacttccc ttgaaaatct aggcgccttg
    16861 attgaatctg aaagcggaac aattattgaa aaaggataag ttgttttact aataagatgt
    16921 attctatttc tagtatcttt atatcaaatt agaaattatt cttgaaaaca tgtacaatat
    16981 ttcaaaagat attagttttt tagactttaa tatggtaaaa caaatataaa tagaaagcgt
    17041 tttcttgaat gtttatttaa gaaagtagtt ggttttttac actttgttag acatcaggag
    17101 gaaaaacaaa tgagtgaaaa agctaaaaaa gggtttaaga tgccttcatc ttacaccgta
    17161 ttattgataa tcattgctat tatggcagtg ctaacttggt ttatccctgc gggggccttt
    17221 atagaaggta tttacgagac tcagcctcaa aatccacaag ggatttggga tgtcctcatg
    17281 gcaccgattc gggctatgct aggtactcat ccagaggaag gttcgctcat taaagaaacg
    17341 agcgcagcga ttgatgtagc cttcttcatc cttatggttg ggggtttcct tggcattgtc
    17401 aacaaaactg gtgctcttga cgtagggatt gcctctatcg tgaagaagta taagggccgc
    17461 gaaaaaatgt taattttggt actgatgcct ttgtttgccc tcggtggtac aacttatggt
    17521 atgggggaag aaacaatggc cttctatcca ctccttgtgc cagttatgat ggccgttggt
    17581 tttgatagcc tgactggtgt tgcaattatt ttgctcggtt ctcaaatcgg ctgtttggca
    17641 tctactctga atccatttgc gacaggtatt gcttcagcga ctgcgggagt tggtacaggg
    17701 gacggtatcg tacttcgtct gatcttctgg gttaccctga ctgctcttag tacttggttt
    17761 gtttaccgtt atgcggataa gattcaaaaa gatccgacta agtcactggt ttatagtact
    17821 cgcaaagaag atttgaaaca ctttaacgta gaagaatctt catctgtaga atctacactt
    17881 agcagcaaac aaaaatcagt tctcttctta tttgtgttga cattcatctt gatggtattg
    17941 agcttcattc catggacaga ccttggcgtt accatttttg atgactttaa tgcttggttg
    18001 actggtcttc cagttattgg taatattgtc ggttcatcta cttctgcact aggtacttgg
    18061 tacttcccag aaggcgcaat gctctttgcc tttatgggta tcctgattgg tattatttat
    18121 ggtcttaaag aagataagat tatctcttcc ttcatgaatg gtgctgctga cttgctcagt
    18181 gttgccttga tcgtagcgat tgctcgtggt attcaagtta tcatgaacga cggtatgatt
    18241 accgatacaa tcctcaactg gggtaaagaa ggcttgagcg gtctatcttc acaagtcttt
    18301 atcgttgtaa cttatatctt ctatctacct atgtcattct tgatcccatc ttcatctggt
    18361 cttgccagcg caactatggg tatcatggct ccacttggag aatttgtaaa tgtccgtcct
    18421 agcttgatta tcactgctta ccaatctgct tcaggtgtct tgaacttgat tgcaccaaca
    18481 tctggtattg tgatgggagc tcttgcactt ggacgtatca acattggtac ttggtggaaa
    18541 ttcatgggca aactcgtagt cgctattatt gtagtgacca tcgcccttct tctccttgga
    18601 accttccttc cattcctata aaatagtgag tgaggtgatt tcatgaaaat agatataaca
    18661 aatcaagtta aagatgaatt tcttatatca ttaaaaacct tgatttccta tccttcagta
    18721 ctcaatgaag gagaaaatgg aacacctttt ggacaagcaa tccaagatgt cctagaaaaa
    18781 actttagaga tttgtcaaga cataggtttc actacctatc ttgaccctaa aggttattac
    18841 ggatatgcag aaatcggtca gggagcagag cttctggcca ttctctgtca tttggatgtt
    18901 gttccatcag gtgatgaagc agattggcag acaccgccat ttgaagcaac tatcaaagac
    18961 ggctgggtat tcggacgtgg tgtccaagat gataaaggcc cttcgctcgc agctctctat
    19021 gcagtaaaaa gcttgctgga ccaaggtatt cagttcaaaa agcgcgtacg ctttatcttt
    19081 ggtaccgatg aggaaaccct ctggcgctgc atggcacgct acaataccat cgaagaacag
    19141 gccagcatgg gctttgcacc tgactcatct tttcctctga cctatgctga aaaagggctt
    19201 ctacaggtca aacttcatgg ccctggatcg gatcaactag agcttgaagt aggaggcgcc
    19261 tttaacgttg taccagacaa ggccaactac caaggtcccc tctatgaaca ggtttgtaac
    19321 ggtctcaaag aagctggtta tgattaccaa tccactgaac aaaccgtaac ggttctcgga
    19381 gtgccaaagc atgctaagga tgctagtcaa ggtatcaatg ctgtcatccg actagctacc
    19441 attcttgctc ctctccaaga acaccctgct ctcagttttc ttgcaacaca agcaggtcaa
    19501 gacggcacag gaagacaaat ctttggtgat atagcagatg aaccttctgg tcacctatcc
    19561 tttaatgtcg caggtctcat gatcaatcat gaacgttctg aaatccgtat tgacattcgg
    19621 actcctgtct tagctgataa ggaagaacta gtagagttgc ttacaagatg tgcacaaaac
    19681 taccaactcc gctacgaaga gtttgactat ctagcgcctc tatacgtcgc aaaagacagt
    19741 aaactcgtta gcacactgat gcaaatctac caagaaaaga ctggcgataa cagtcctgct
    19801 atttcatccg gtggtgccac ttttgctcgc accatgccaa attgtgtagc cttcggcgcc
    19861 ttattcccag gagcgaagca gacagaacat caggcaaatg aatgtgccgt tctagaagat
    19921 ttgtaccgtg ctatggatat ttatgccgaa gccgtctatc gacttgcaac ttaatcaggc
    19981 aactgtttct accaaaaaaa atcgaccgat taatgaactg caccccaaaa gttagacaga
    20041 ataaatctaa cttttggggt gttttattat gaaattgagt tatgaagata aagttcagat
    20101 ctatgaacta agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttggtgtgga
    20161 tgtttctggt ctaaagtaca tggtgaaatt aattgaccgt tatggcatag aaatcgttaa
    20221 aaaagggaag aatcgtcact attcttctaa attgaaacaa gaaatgatgg ataaagcctt
    20281 actggaaggt tgttcacaaa gaagtattag tcttgattat gccctcccaa accaaggaat
    20341 gctttcattt tggccggcac aatacaagaa aaacgggtat actattgttg agaaaacaag
    20401 agggagacca gctaaaatgg gacgtaaacg gaagaagact tgggaagaaa tgacagaact
    20461 agaacgactc caggaggaga atgaacgctt acggactgag gtggcctacc taaaaaagtt
    20521 aaaagagtta gaggaaaggg acgaagccct agagcgagaa aggcagagac agttagagaa
    20581 atggtttcag gaggatttcg actagatttt cttcttgaaa cagcacgttt agctcgctcg
    20641 acttactatt atcagttgaa acaactggat ggggttgata aagataaaga gattaaaacc
    20701 gaaattcagg gcattgataa tgaacataaa ggcaactatg gctatcgtcg aatccattta
    20761 gaactaagga atcgtggttt tgtggtcaat cataagaaag ttcaacggtt gatgagaatt
    20821 cttggtttaa cggctcgaat tcgccgtaaa cggaagtatt cttcctacca aggagagatt
    20881 ggcaagaaag cagagaatct cattcaacgc cagttcgaag catcgaggcc aatggaaaag
    20941 tgctatacag atgtgacaga gtttgccatt ccaaatagca cacagaaact ctatttatca
    21001 ccagttttag atggttttaa cagtgaaatc attgcttatc atctttctac ttcgccaaac
    21061 ttagaacaag tgaagagtat gttagaacag gcctttacag agaaatacta tgagaatacg
    21121 attctccata gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag
    21181 agtaagggaa ttcaagcatc catgtcacgc aagggcaaca gcccagacaa cagcatgatg
    21241 gaatctttct ttggcatttt aaaatccgaa atgttttatg gctatgagaa gaactttaga
    21301 tctttagaaa accttgaaca agctattgtg gactacattg attactacaa caacaaacga
    21361 attaaggtaa aactaaaagg acttagttct gtgcagtaca gaactaaatc ctttggat
//
LOCUS       DAVMEF010000015        21080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMEF010000015 DAVMEF010000000
VERSION     DAVMEF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.015"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            400..633
                     /locus_tag="VYQ30_000412"
     CDS             400..633
                     /locus_tag="VYQ30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1965576.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            916..2865
                     /gene="mutL"
                     /locus_tag="VYQ30_000413"
     CDS             916..2865
                     /gene="mutL"
                     /locus_tag="VYQ30_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1965577.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLASA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3190..3657)
                     /locus_tag="VYQ30_000414"
     CDS             complement(3190..3657)
                     /locus_tag="VYQ30_000414"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1965578.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3658..4863)
                     /locus_tag="VYQ30_000415"
     CDS             complement(3658..4863)
                     /locus_tag="VYQ30_000415"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1965579.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(4883..5518)
                     /gene="ribE"
                     /locus_tag="VYQ30_000416"
     CDS             complement(4883..5518)
                     /gene="ribE"
                     /locus_tag="VYQ30_000416"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1965580.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5503..6603)
                     /gene="ribD"
                     /locus_tag="VYQ30_000417"
     CDS             complement(5503..6603)
                     /gene="ribD"
                     /locus_tag="VYQ30_000417"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1965581.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAIIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(6737..6851)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7008..7601
                     /gene="ruvA"
                     /locus_tag="VYQ30_000418"
     CDS             7008..7601
                     /gene="ruvA"
                     /locus_tag="VYQ30_000418"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1965582.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQAVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7611..8174
                     /locus_tag="VYQ30_000419"
     CDS             7611..8174
                     /locus_tag="VYQ30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1965583.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8171..8848
                     /locus_tag="VYQ30_000420"
     CDS             8171..8848
                     /locus_tag="VYQ30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1965584.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIVNYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9003..10034)
                     /locus_tag="VYQ30_000421"
     CDS             complement(9003..10034)
                     /locus_tag="VYQ30_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1965585.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTERIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10289..10972)
                     /locus_tag="VYQ30_000422"
     CDS             complement(10289..10972)
                     /locus_tag="VYQ30_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1965586.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(10984..11928)
                     /locus_tag="VYQ30_000423"
     CDS             complement(10984..11928)
                     /locus_tag="VYQ30_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1965587.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12060..14891
                     /gene="uvrA"
                     /locus_tag="VYQ30_000424"
     CDS             12060..14891
                     /gene="uvrA"
                     /locus_tag="VYQ30_000424"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1965588.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            14884..15945
                     /locus_tag="VYQ30_000425"
     CDS             14884..15945
                     /locus_tag="VYQ30_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1965589.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16077..16475
                     /gene="spx"
                     /locus_tag="VYQ30_000426"
     CDS             16077..16475
                     /gene="spx"
                     /locus_tag="VYQ30_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1965590.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16541..17110
                     /locus_tag="VYQ30_000427"
     CDS             16541..17110
                     /locus_tag="VYQ30_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1965591.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSNYISDRLANGATEQ"
     gene            17197..17463
                     /locus_tag="VYQ30_000428"
     CDS             17197..17463
                     /locus_tag="VYQ30_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1965592.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17467..17886
                     /gene="ruvX"
                     /locus_tag="VYQ30_000429"
     CDS             17467..17886
                     /gene="ruvX"
                     /locus_tag="VYQ30_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1965593.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            17902..18207
                     /locus_tag="VYQ30_000430"
     CDS             17902..18207
                     /locus_tag="VYQ30_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1965594.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18462..19712
                     /locus_tag="VYQ30_000431"
     CDS             18462..19712
                     /locus_tag="VYQ30_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1965595.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            19709..20254
                     /locus_tag="VYQ30_000432"
     CDS             19709..20254
                     /locus_tag="VYQ30_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1965596.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKSIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            complement(20319..>21080)
                     /locus_tag="VYQ30_000433"
     CDS             complement(20319..>21080)
                     /locus_tag="VYQ30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1965597.1"
                     /translation="DEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIRDHFLKYDRA
                     VRCRVKIITMDMFSPYYGLAKQLRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKR
                     YWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSKDLKHHYQLYQLLLFHFQN
                     KEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLI
                     KRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         6146 a         4083 c         4567 g         6284 t
ORIGIN      
        1 taatgactat aactgaaaaa gaaaagtgat atctttaagg ttgttcttct tggtggtgag
       61 attcgtgaga caacccaagc ttttgtcgga aagattacca atgctttgat ggataggatg
      121 tactttagca agatgttttt agtggtaacg gtatcgtgga tggacgtgta ataacctctt
      181 ctttcgagga gtattttact aaaaaactag ccttggagcg ttccccagaa acggacttac
      241 tcattgactc ttcaaagatt tggggagaag attttgcttc atctgttcct tgaaaaaagt
      301 cacagcagtc atcacagacg atagtactga acaaaactat gaagagttag aaatttatac
      361 gcaggtgatt gtataaagga tctggaaata gataagaagt tgattagtat tgacctaggt
      421 ggtacaaata ttaagattac tgttctttca aatgacggtg agattgaaac tttgtggagt
      481 attacaacag atacaagtga gaaaggttct caaattatat cggacatcat cagttctatt
      541 aaaaataaat tgaccgaacg gaatattcct gatagcgacc ttcttggaat cggtatggga
      601 agttgctcat catactttcc ttgtaaatca taggggctat aaactctccg tctacttgtc
      661 ctacaacaat tgaagtctgc tcaaaacgcc gtccgctaat cttttcatag actttctccc
      721 ttttaggagc ctagctttct agtttgttct ttgattttta ttgagtatac cactatttta
      781 ctccctctgg caagggactt tgtctatgtg gagggattgg gctcctatgt ggtggagctt
      841 ttctgttctt tctgaaatat ggtataatag cactaatcaa tttctaggaa aatagataca
      901 gaaaggggct gaaagatgtc tcatattatt gaattgccag agatgctggc aaaccagatt
      961 gcggctggag aggtcattga acgtcctgca agtgtggtta aagagttggt agaaaatgcc
     1021 attgacgcgg gctctagtca gattatcatt gagattgagg aagctggtct caagaaaatc
     1081 caaatcacag ataacggtta tggaattgcc cacgatgagg tggagttggc cctgcgtcgc
     1141 catgcgacca gtaagataaa aaatcaagca gatctctttc ggattcggac gcttggtttt
     1201 cgtggtgaag ccttgccttc tattgcgtct gttagtgtct tgactctgtt aacggcggtg
     1261 gatggtgcta gtcatggaac caagttagtc gcgcgtgggg gtgaagttga ggaagtcatc
     1321 ccagcgacta gtcctgtggg aaccaaggtt tgtgtggagg atctcttttt caacacgcct
     1381 gcccgtctca agtatatgaa gagccagcaa gcggagttgt ctcatatcat tgatattgtc
     1441 aaccgtctgg gcttggccca tcctgagatt tcttttagct tgattagtga tggcaaggaa
     1501 atgacgcgaa cagcagggac tggtcaattg cgccaagcaa tcgcagggat ttacggtttg
     1561 gcgagtgcca agaagatgat tgaaattgag aactctgacc tagatttcga aatttcaggt
     1621 tttgtgtcct tgcctgagtt gactcgggct aaccgcaatt atatcagcct cttcatcaat
     1681 ggccgttata tcaagaactt cctgctcaat cgtgctattt tggatggttt tggaagcaag
     1741 cttatggttg gacgttttcc actggctgtc attcacatcc atatcgaccc ttatctagcg
     1801 gatgtcaatg tgcatccaac taagcaagag gtgcggattt ccaaggaaaa agaactgatg
     1861 actctggttt cagaagctat tgcaaatagt ctcaaggaac aaaccttgat tccagatgcc
     1921 ttggaaaatc ttgccaaatc gaccgtgcgc aatcgtgaga aggtggagca aactattctc
     1981 ccactcaaag aaaatacgct ctactatgag aaaactgagc cgtcaagacc tagtcaaact
     2041 gaagtagctg attatcaggt agaattgact gatgaagggc aggatttgac cctgtttgcc
     2101 aaggaaacct tggaccgatt gaccaagcca gcaaaactgc attttgcaga gagaaagcct
     2161 gctaactacg accagctaga ccatccagag ttagatcttg ctagcatcga taaggcttat
     2221 gacaaactgg agcgagaaga agcatccagc ttcccagagt tggagttttt cggacaaatg
     2281 cacgggactt atctctttgc ccaagggcga gatggacttt acatcataga tcagcacgct
     2341 gctcaggaac gggtcaagta cgaggagtac cgtgaaagca ttggcaatgt tgaccagagc
     2401 cagcagcaac tcctagtgcc ctatatcttt gaatttcctg cggatgatgc cctgcgtctc
     2461 aaggaaagaa tgcctctctt agaggaagtg ggcgtctttc tagcagagta cggagaaaat
     2521 caatttattc tacgtgaaca tcctatttgg atggcagaag aagagattga atcaggcatc
     2581 tatgagatgt gcgacatgct ccttttgacc aaggaagttt ctatcaagaa ataccgagca
     2641 gagctggcta tcatgatgtc ttgcaagcga tctatcaagg ccaatcatcg tattgatgat
     2701 cattcagcta gacaactcct ctatcagctt tctcaatgtg acaatcccta taactgtcct
     2761 cacggacgtc ctgttttggt gcattttacc aagtcagata tggaaaagat gttccgacgt
     2821 attcaggaaa atcacaccag tctccgtgag ttggggaaat attaaaagta taaaaaagtc
     2881 tgggaaaaat tttcaaaatc agaaaaacgc ataaaatcag gtgttcaaaa accttgattt
     2941 tatgcgtttt atcatagaaa tatttacttc attttttcct aattcttttc gaaactcttt
     3001 ttaaaccacg tcagttttat cagtaatctc aaaacagtgt tttgagctaa ttttgccagt
     3061 tttgtctgta acatcgaagt tgtgtttgac cactctgcga ctggtttcct agtttgctct
     3121 atgattttca cagagtatta aattgcgatt ttgccaagtt tctttattcg tctaaaagta
     3181 gagtctgttc tatgcgtcta atgtacgaat taggttgacc atttcaatag ctccttgtgc
     3241 acactcagaa cccttatttc ctgctttagt gccagctcgt tctatggctt gttcaattgt
     3301 atctgtcgtt agcacaccaa acataacagg aatttcgcta tttaaactga tttgggcgat
     3361 tcctttagat acctcgctac atacataatc ataatgactt gtattccctc taatgacagc
     3421 tcccaagcag ataattgcat catatttttt actttttgcc atttttgatg caatcagtgg
     3481 tatttcaaaa gctcctggaa cccaggctac ctcgatatct ttctcgttta cattctctct
     3541 tttgagatta tctagtgctc cagataataa ttttgaagtt ataaattcat taaatctcgc
     3601 tacaacaata cctattttaa tattgtttgc tactaaatta ccttcataag tgttcattta
     3661 tttttcctcc atatttaaaa tgtgacccat tcgatttttc tttgtttcta aataaaaact
     3721 atcgtaagga ttggcttcta tttcgattga tattctactg gaaatggtaa ttccatattt
     3781 ttctaactgt tcaaccttgt caggattatt tgtcagtaaa tgaagtgact gaagtcccag
     3841 atctttaagc atttgtgctc caatatgata ttctcttaaa tcaccttcaa agcctaatgc
     3901 aagattggca tcaagcgtat ccatgccttg atcttgtaaa tgataggctt ttaatttatt
     3961 gataagtcca attcctcgtc cctcctgtcg caagtaaagt aagacacccg aaccattctc
     4021 aacaatcatt ttcatagctt tatcgaattg ctgtccacaa tcgcaacgta aagagcctaa
     4081 aacatctcct gttaaacatt cggagtggac ccgacataat acattggctt catcctctat
     4141 gtttcccata ataagagcaa gatgatgttc cccatttagt ttatctatat agctaattgc
     4201 tttgaaatta ccgtatctag taggcatatt gacagttgaa actcgttcta ccagctgatc
     4261 atatactttt ctatattctt gtaattcttt gatggtaatt agtggaatgt tgtgtttctt
     4321 cgagaactga attaaatcat ctgttctcat cattttgcca tcatgattca ttatttcaca
     4381 acataggcca cactctttta gtccagctaa ttttaataaa tcaacagttg cttctgtgtg
     4441 tccatttctt tctagaacac cacctttttt tgcaattaaa ggaaacatgt gtcctggcct
     4501 gcgaaaatca gagggtgtta tatcttcagc tacacacata cgtgcggtca gtcctctttc
     4561 ctcggcagaa atacctgtgg tcgtttcttt ataatcaatt gaaactgtaa aagcagtctt
     4621 atgattatct gtattgtttt caaccatagg tgaaagcatt aattgattag ctaaactttc
     4681 gctcataggc atacaaatta atcctttggc ataagtagcc ataaaattaa cattttctgt
     4741 tgtagctgct tgtgcagaac aaattaagtc tccttcattt tctctatcct tgtcgtctat
     4801 aacaagaaca agtcgtccct tctgcaatgc ttctaatgct tcttgtattt ttcgatattc
     4861 cattgactga ttatcctttc tgctaaaatc cattttgata taatagttcc ttcgatattt
     4921 ctgattttgg agagttatcc atcagttttt gcacatattt acctaagata tcattttcaa
     4981 gatttactgt actcccgact tgtttactct taagaatggt ttgttccaag gtatgaggga
     5041 taacagatac tgaaaagttt actttggaga ctttagcgac agtcagacta atgccgtcaa
     5101 ttgtaataga tcctttttca actattaaat ctaaaatttc tttttgtgtg ttgatttgat
     5161 accatacagc attatcatct ttttttattg acgagatttt tcctgtacca tcaatgtgtc
     5221 ctgtaacgac gtgaccccca agtcgaccgt tgacagataa ggctctttct agattcacct
     5281 cacttccatg ttttaataga gtaagagctg ttcgactcca tgtttcattc attacatcaa
     5341 ctgtaaagga ttgatgattg aaatgagtaa ctgtaagaca gataccattt actgctatac
     5401 tatcgcctaa atggatatcc gttaatattt ttgaggcttt aattgatagt ttacaattac
     5461 gagagtcttt ctgtattctt tcaacttttc cgatttcttc aattattcct gtgaacatgg
     5521 ataaatcact tcactttcta tgagatagtc atttcctatt tgagaaaaag cataaggttt
     5581 caatctaata gcgtcatttg gcaaagaaat gccttcacct ccgacaggaa acttggcact
     5641 gcctccaaaa atttttggtg caatatatat tttcagctca tcaacaattt gttgttccaa
     5701 agcactccaa ttcattagac tgcccccttc tagaactagg ctatcaatct gcatgtttcc
     5761 tagatgttgc attaaactcg ataagtctat atgattgcct tttttcttta tggaaagtat
     5821 ttcacagcca tgattttgat atagcttcat tttatttttg tcttcagagg aagtggcaat
     5881 gtaagtttta atatcatttg ctgtttttac gattttagag gtaagaggag ttcgtaaatg
     5941 tgtatcgcat atgatacgga taggattttt cccttcctcc aatctacatg tcagcaaagg
     6001 atcgtcttga ataacagtat tgactcccac cataattgca ctaacatggt gtcgtaactg
     6061 atgcacgtgc tttcttgctt cttcttcagt aatccatttg gattgatttg ttttagtggc
     6121 tatttttcca tccattgaca ttgcatattt cataaaaaca tagggtacat gctgggtaat
     6181 atactttcta aaacttttta ttaagttaag acactcattt tctaaaattc caacagtaac
     6241 ttgaagatta ttttcctcaa gtatctttac tccttttcca gatacaatag gattacagtc
     6301 taggcttcca atgactactc ttgtaatacc actatcgatt atagcatcta tacagggagg
     6361 tgttttcccg aagtgacaac agggttcaag tgttacataa agcgtcgctc cgacagggga
     6421 ttttctacag tttttaagag catttctctc agcatgtggg ccaccaaaaa actcatgata
     6481 accttgtccg ataatgtgat tatcttttac aataattgcg ccgaccatag gattggggtt
     6541 gacgtaacca gccccttttt gtgccagttt tattgctaat ttcatatatt ttgaatcgct
     6601 catctcacta cctccaaaaa aatatacctt gaatagggga ctactcaagg catacaaaag
     6661 aaaacttatg cgattaacaa aaatgctctg aaatgacaag taatcatttc agagcacgca
     6721 aaaagcacaa atatactttt atcttctttc atccagacta tactgtcggc tttggaattt
     6781 caccaaatca tgccttttgg ctcgtgggct ataccaccgg tagggaattg caccctgccc
     6841 tgaagatagt tattcaatta cagatgatta tagtacttaa ttttgaatat gtcaacagat
     6901 aaataccgat tgtttttgat atactgtatt tgtgataatc gattctcgct cctcggataa
     6961 agaaaatatg atatactaga taaacgaaat aagagagaag gaacactatg tacgcatatt
     7021 taaaaggaat cattaccaaa attactgcca aatacattgt tcttgaaacc aatggtattg
     7081 gttatatcct gcatgtggcc aatccttatg cctattcagg tcaggttaat caggaggctc
     7141 agatttatgt gcatcaggct gtgcgtgagg acgcccattt gctttatgga tttcgctcag
     7201 aggatgagaa aaagctcttt cttagtctga tttcggtctc tgggattggt cctgtatcag
     7261 ctcttgctat tatcgctgct gatgacaatg ctggcttggt tcaagccatt gaaaccaaga
     7321 acatcaccta cttgaccaag ttccctaaaa ttggcaagaa aacagcccag cagatggtgc
     7381 tggacttgga aggcaaggta gtagttgcag gagatgacct tcctgccaag gtcgcagtgc
     7441 aagcaagtgc tgaaaaccaa gaattggaag aagctatgga agccatgttg gctctgggct
     7501 acaaggcaac agagctcaag aaaatcaaga aattctttga aggaacgaca gatacagctg
     7561 agaactatat caagtcggcc cttaaaatgt tggtcaaata ggagcagaga atgacaaaac
     7621 gttgttcgtg ggtcaagatg accaacccgc tctacatcgc ctatcatgat gaggagtggg
     7681 gccagcccct ccatgatgac caagcattgt ttgagttgtt gtgtatggaa acctatcagg
     7741 caggcctgtc ttgggaaacg gtactcaaca aacgccaagc tttccgagaa gcctttcata
     7801 gctatcaaat tcactcagtc gcagagatga ctgacactga attggaagcc atgctggaga
     7861 atccagctat cattcgaaat agagccaaga tttttgctac acgcgctaac gcccaagcct
     7921 ttctacagtt acaggcagag tacggctctt ttgatgccta tctttggtct tttgttgagg
     7981 ggaaaactgt cgttaatgat gttcctgatt atcgccaagc tccagctaaa acacccttat
     8041 ctgagaaatt agccaaagat ctcaaaaaac gaggcttcaa gttcacaggc ccagtcgccg
     8101 tattgtcttt tctacaggct gcagggctag ttgatgacca cgagaatgat tgtgagtgga
     8161 aaggtcttaa atgatgtcta acaaaaataa gggaattctg atttttgcga ttctctatac
     8221 agtcctcttt gtgtttgatg gcgttaaatt gctggcttct ttaatgccat ctgccattgt
     8281 aaattatctt gtttatgtag ttttagctct atatggctcc ttcttgttca aggatagatt
     8341 gatccaacaa tggaaggaga ttagaaagac taaaagaaaa ttcttctttg gcgtcttaac
     8401 aggatggctc tttctcattc tgatgactgt tgtctttgaa tttgtatcag agatgttgaa
     8461 gcagtttgtg gggctagtcg gacaaggtct aaatcagtct aatattcaaa gcacttttca
     8521 agagcaacca ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg tagaagaatt
     8581 atttttccgt caggtcttat tgcattactt gcaggaacgg ttgccaggtt tactaagcat
     8641 tattctggta ggacttgttt ttgctctgac tcatatgcac agtttggctc tatcagagtg
     8701 gattggtgca gttggttact taggtggagg ccttgccttt tctattattt atgtgaaaga
     8761 aaaagagaat atctactatc ccctacttgt tcacatgtta agcaacagcc tctccttaat
     8821 cattttagct atcagtatag taaaatgaaa taagaacagg acaaatcgat caggacagtc
     8881 aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt caatttgctg
     8941 taatatgtga tggaaatgcc agtaatgata ccgagaaaaa agctgagaaa cttttcccag
     9001 ctttatttgt tatagtcaaa gcgaatgact tgttcctgtg catctacatg agcatggacc
     9061 ccaaagggta caattgctct tggagttgcg tggccggcat tcagattata gacaatcggg
     9121 atattgctgt caatgatatc caatagtgcc tctttatagt cgtcatggaa ggtttcatcc
     9181 ataggttttc cgaccaagag tccattgatg accgcgaata tgccagtgtc ctttaaagtt
     9241 agcaacatct ttttgaagtc ttctggctta ggcttttctt cgcttgtttc gagcaagagg
     9301 attttccctt cccagtctga caagtcaggg aaaagtttgt atttttggca gagttccgtg
     9361 ctatctgcgt atcgagagtt gtcaaagata tcgtagaggg attcgaggca accaccgagg
     9421 attttcccct cgaactgagc acttccttgc aacaagtcaa aacctgtatt tgtatgactg
     9481 acacgaggtg ttcccagggc cgtgggacta aaatcagttc gttcctcata ccaaacgtca
     9541 ctagggcgga tttctgaaat tctttccgtc ttaatcaatt ctttaaagta gtgaaggcta
     9601 taggctagca tttctttgtc taattcacaa atgtctgcta aaaaggattg accataaaaa
     9661 gtcttgattc ctaatttatg caacatgagg tggttcatgg ttgtatccga gaagccaaga
     9721 aaaatttttt gtttgataac cttttggagt tggtcatttt caaaaagata aggtagcaag
     9781 cgataggtat cgtctccacc gatggcacat aggatcatgt cgatgctatc atcagaaaag
     9841 gcatgaatca aatcctctgc acgagcttca ggatggtcct tgataaagtc taatcctttt
     9901 aacgaatggg gcaaaaagat gggattgagt cccagatcct tgagacgttg gatacccaag
     9961 tccacttcgt gtttgacaaa atcctctccg ataatgccac tagataaact aacaatacca
    10021 atagtagaaa ccatatctca tcctcctaga aatagattga gcctatttta tcacaaaata
    10081 taagagaaag ctatgtggga tttcagaaga atacttttaa tactcttcga aaatctcttc
    10141 aaaccacgtc agttttatct gcaacctcaa agctgtgctt tgagcaacct gcagctagct
    10201 tcctagtttg ctctttgatt ttcattgaat ataactcaaa aaagtaagtg aaaaagcaac
    10261 cgcacctgca gttgctttta tcgttttctt attcttgata gcgtgttggt cctgcacttg
    10321 cactacggat attcaagcgt ttcttgagat agaagcgaag ggctaggagg gctgctccaa
    10381 taatagctag tggcaatgga tccagtactg ggttaaggct agctggtagg aagcttgttg
    10441 caaagaagac aagcaaccaa aggaacatag aagctaggat aactagtaca gatttccaga
    10501 aaggtggacg ttgactgcga tccatatctg gtccatagta ttggtaaaca aagtagtaca
    10561 tcaagtagaa ggcaaatcca ccaaccagtc caactaatag aagagtaatc aatccatagc
    10621 cgaaagcttg gtctgccgca aagaaggttg tgagggcgct gacaagggca aagaggctag
    10681 tgatgaaaag agctgagtcc ataatcatca gttttgggtc atcattttct tttggatgct
    10741 ctttttcgta ctgctctttg acagtgaagc tatgagccca atgagttggt gcgccgtata
    10801 gggaacgggc agttgtacct ttagattgct cctcaaggat ttggggaatg acttcctcaa
    10861 aaatagtctg gatttcagcg tctgttttcc catctttgat gaattgttgg gtagcaatgt
    10921 ggacaaactc ttggtttttc ttagttaatt tttgtagatc aatttgagac ataggaactc
    10981 ctcttagaac cattttttat ggatgagata gagagtgagc gagacactca tagcaaaggc
    11041 gataaagacg attaaccaga aggcatttgg ctctccgttt agggggattt cattatcctt
    11101 aaagttcatc ccgtaggcag aaaagaccat ggttgggatg gacatgacga tggtcacaag
    11161 ggccaaggtt ttcatgatgt tgttctggtt gttagaaatg atagaggcaa aggtctctgt
    11221 catagaatgc aagacgtttc cataaatatc tgccatctcg atggcctgtt gggtttcaat
    11281 cagggtgtct tcaagcaggt cttcgtcctc aaggtatttc ttgatattgc tggttgaact
    11341 ggtcaatttc ttaatcacgc gctcatttgt tttgagggag gccttgaaat agacgatagt
    11401 tttttccaat tccatgagct caatcaattc ttcattacga gttgattgat gcagttgact
    11461 ttcgatttgt tcactcttgc ggtcgattga acgaagggct gttaggtaaa gctctgcatt
    11521 gcgataaaga atttgaaaga taaaacgtga acgcatgaag gtatagaaat tacgcaatcg
    11581 acggttgata aagacatcaa ggacaggtag tggttccaaa cacgtagtga taatggtttc
    11641 ctcagtgatg ataataccaa gcgggatggt tacgtagtag gtgcggttat ttctttcctc
    11701 cgtgaccggc acgtctacga taatcagggt atactcgtct tcaatggtaa tacgagacat
    11761 ttcttccgca tcgagcggtg ctcgaaggtc ggcaatatca atatcgaagg tgttggcgat
    11821 ttcgagtgat tcattttgag tcggattgac gagattgatc caagtacccg gttcaagcgt
    11881 atcgatctct ttaaattcag ttgttgtaga gagaaaaact tgtttcatat cccttatcct
    11941 ttctcatttt tcagattttt tcacactgta ctattatact acaaaatcgg ccttttgggt
    12001 aatagaatct cacttttttt ggtataatgg taagcaataa tggactagaa agaacaaaga
    12061 tgcaagataa aattgtcatt catggggcgc gtgcccataa tttaaaaaat attgatgtgg
    12121 agattccgcg agacaagttg gttgtcgtga ctggcttgtc aggttcaggg aagtccagtc
    12181 tggcttttga tactctctat gcggagggac aacgtcgcta tgtagagagt ttgtccgcct
    12241 acgctcgtca gttcttggga aatatggaaa aaccagatgt agatgccatt gatggtctca
    12301 gcccagctat ttccatcgac cagaaaacga ctagtaaaaa tcctcgctcg acggtgggaa
    12361 cgacgactga aatcaatgac tatctgcgtc tcctctacgc acgtgtgggg acgccttact
    12421 gtatcaacgg acatggagct atcaatgctt cttctgtgga gcaaatcgtt gataaggttt
    12481 tggagttacc tgaacgccag cgcttgcaga tcttggctcc tgtcatccgc aagaaaaaag
    12541 gccaacataa gagtgttatc gagaaggttc agaaagacgg gtatgttcgt gttcgtgtgg
    12601 atggggaagt ctatgatgtg accgaagtgc cagagttgtc taagagcaag caacacaata
    12661 tcgatgtcgt ggttgaccgt attgttatca aggagggcat tcgtagtcgt ctctttgatt
    12721 ccattgaggc tgcccttcgt attgcagaag gttatgtcat tatcgacacc atggacgatt
    12781 cggagttgct cttctctgag cattatgcct gtccagtttg tggttttact gtcccagagt
    12841 tagagcctcg tctcttctct ttcaatgctc cttttggctc ttgtagtgag tgtgacggct
    12901 tgggcatcaa gctggaggtg gatactgatt tggtagtgcc agatgccagc aaaaccttac
    12961 atgagggagc gctggcacct tggaatccta tctcatccaa ctactatcca aacatgttag
    13021 agcaggccat gaaagtcttt ggagtggcta tggataagcc ttttgaggac ctgtcagaag
    13081 aagataagaa cttgattctc tacggctcag atggtaagga attccatttc cattatgaaa
    13141 atgaatttgg tggtgtgcgc gatatcgaca ttccgtttga gggagttatc aataatatca
    13201 aacgtcgtta ccatgaaacc aatagcgatt acactcgcac tcagatgcgt ctctacatga
    13261 atgagctgac ctgcggaacc tgtcaaggct atcgtctcaa tgaccaggcc ttgtctgtcc
    13321 gtgtgggcgg ccagcaaggg ccacatattg gagaaatctc agacctgtct atcgctgacc
    13381 acttggactt ggtgagccag ttaactttgt ctgaaaatga agccatcatt gctcgtccca
    13441 ttctcaagga aatcaaggat cgtttgacct tccttaataa tgtgggtctt aactatctaa
    13501 ccctgtcacg ttcagcagga accctttcag gtggggaaag tcagcgcatt cgtttggcaa
    13561 cccagattgg ttccaaccta tcaggcgtcc tctatatcct agacgagccg tcaatcggtc
    13621 ttcaccagag ggacaatgac cgcctgattg ccagtctgaa aaagatgcgt gacttgggca
    13681 atactcttat cgtggtggaa cacgacgaag ataccatgcg tgaagctgat tatctgattg
    13741 acgttggtcc tggtgccggt gtttttggtg gtgagattgt tgcagcaggt acacctaagc
    13801 aagtggctcg caacagcaag tctatcacag gtcagtactt gtcaggcaaa cgtgtcatcc
    13861 cagtaccaga agagcgccgt gtcggaaatg gtcgttttat tgaagtgata ggagcgcgtg
    13921 agaacaactt gcaaaatgtc acggctcgct ttccactagg aaaatttatc gcagtaacag
    13981 gtgtgtcggg ttcagggaaa tcgactctaa tcaacagcat tctcaaaaaa gccattgccc
    14041 agaagctcaa ccgtaattca gacaaacctg gtaagtttaa gactattaca gggattgagc
    14101 atgtcgaccg tttgattgat attgaccaga gccctatcgg acggacgccg aggtctaacc
    14161 cagctaccta tacaggagtt tttgacgata tacgtgacct ttttgctcag acaaatgaag
    14221 ccaagattcg aggctacaag aaggggcgct tcagtttcaa cgtcaaggga ggtcgctgtg
    14281 aagcttgctc aggtgacggg attatcaaga ttgagatgca cttcttgcca gatgtttatg
    14341 tggcttgtga agtttgccac ggaacccgct ataacagtga aaccctagaa gttcactaca
    14401 aggaaaagaa tatttcgcag gtcttggata tgacggtcaa cgatgcggta gaatttttcc
    14461 aacacattcc gaaaattcaa cgcaaactcc agaccatcaa ggatgtaggt ctaggctatg
    14521 tgaccttggg acagccagct accacccttt ctgggggaga agcccaacgt atgaagttag
    14581 ctagcgaact ccacaaacgc tcgacaggaa aatctttcta cattctggat gagccgacga
    14641 cagggcttca tacagaggac attgctcgcc tgctcaaggt tttagctcgc tttgtagacg
    14701 acggcaatac agtcctcgtc atcgagcaca atctggatgt tatcaagacg gcagaccata
    14761 tcattgactt gggacctgag ggcggtgtcg gtggtggaac catcatcgta acaggaactc
    14821 cagaagaagt agctgccaac gaagccagct atacaggaca ctatttgaaa ggaaagttac
    14881 atcatgaata aacgcgtaca agcatttcta gctaaaatgc aagaaaaaga actagatggt
    14941 atcatcatca acaatcttaa aaacgtctat tatttgactg gtttttgggg ctcaaacgga
    15001 acagtcttta tcagccgtga ccgtcaggtc ttagtgacag actctcgcta tatcatcgca
    15061 gctaagcaag aaaccagtgg ttttgagatt gtggctgatc gtgatgaatt ggctgtcatt
    15121 gcaggaattg ttaaggacat gaacttgact cgtatcggtt ttgaagatga gatttcagtg
    15181 tcttattacc accgtatgca ggcagctttt gcaggtttga acttgcttcc acaaactcag
    15241 tttgtggaag gtcttcgtat gattaaggat gaggcagaga ttgcagcgat tcgcaaggct
    15301 tgttctatct cagaccaagc tttccgcgat gcgcttgact ttatcaaacc aggaaaaact
    15361 gaaattgaga ttgccaactt ccttgatttc cgcatgcgtg agttgggagc atctggctta
    15421 tcttttgata cgatcctagc tagcggtatc aattcttcta aaccccatgc ccatccaatg
    15481 cacaaaccag tggagttggg agaagccatt accatggact tcggctgtct ctatgaccac
    15541 tatgtcagtg atatgacccg gactatctat ctagggcatg ttagcgatga gcaggcagag
    15601 atttacaata cggttctaaa agctaaccaa gccttgattg accaagctaa ggcaggctta
    15661 ggtttccgtg actttgacaa aatccctcgt gatattatca ttgaggcagg ttatggtgac
    15721 tactttactc acggcattgg ccacggtatt ggtctggata tccatgagga accctacttt
    15781 agtcagactt ctacagaaac tattaagaca ggtatggcct tgaccgatga accaggtatc
    15841 tatatcgaag gcaaatatgg cgttcgtatc gaggatgata tcctgattac agagacaggt
    15901 tgtgaattat tgaccctagc tccaaaagag ttgatagtca tttagttgtt tgtcaagaaa
    15961 aaagtacgaa aatgacgaaa aaagtcaaaa aaaattaaaa ataggtcgca agtcggatgt
    16021 tttttatggt ataatagact aaactgatag taacatgtag cgaaaggggt aggtacatga
    16081 ttaaaattta tacagtctca agttgtacta gctgtaaaaa agcaaaaacc tggctcaatg
    16141 cccaccagtt aagttataaa gaacaaaacc ttggtaaaga aggaattacg agagaagaat
    16201 tactggatat tctaaccaaa acagataacg gaatagccag catcgtttcg tctaaaaatc
    16261 gctatgccaa agcccttgga gtggatattg aagatttgag tgtcaatgaa gttctcaatc
    16321 tgattatgga aacaccgaga attttaaaga gcccaatcct tgtagatgaa aaacgcctgc
    16381 aagttggcta caaggaagac gatattcgtg ccttcctacc acgctctgtc cgtaatgtag
    16441 aaaatgcaga agcacgtttg cgtgcagctc tataaacatc aaggctggga gcacttccca
    16501 gtcttattct attttaattt caaaaagaaa gaagaaagaa atgaaaaaaa tagttcttgt
    16561 tagtctagct ttcctttttg tcctggttgg ttgcggacag aaaaaagaaa ctggaccagc
    16621 tacaaaaaca gaaaaagata cgcttcagtc ggcattgcca gttattgaaa atgctgagaa
    16681 gaatacagtt gtaactaaga ctttggtctt gcccaagtca gatgatggta gccagcagac
    16741 acaaacaatt acttacaaag acaagacttt tttgagtcta gctatccaac aaaaacgtcc
    16801 agtctctgat gagttgaaga cttatattga ccaacatgga gtggaggaaa ctcaaaaagc
    16861 tcttcttgaa gcggaggaga aggataagtc tatcattgaa gctcgtaaat tggcaggttt
    16921 caaacttgaa acaaaactat tgagcgcaac ggaacttcaa acaacgacta gttttgattt
    16981 tcaagttctg gatgtcaaga aggcttccca gttggaacat ctgaagaata ttggtttgga
    17041 aaatcttttg aaaaatgaac caagcaacta tatttcagat agattggcaa atggcgcgac
    17101 agaacaatag aaaatccaaa taaatagact gcctgaattg tagaaggtaa ggaattatgc
    17161 tataatggta ctattctaag gaaagagggt gttagaatgg gatttactga agaaacagta
    17221 cgttttaaat tggacgattc caataaaaaa gaaattagcg aaactttgac agatgtttat
    17281 gcttcgttga acgataaggg ttacaaccca attaaccaaa tcgtaggtta cgtattgagt
    17341 ggagaccctg cctacgttcc tcgttataat aatgcacgaa atcaaatccg taagtatgag
    17401 cgtgatgaaa tcgttgagga attggttcgc tactacctca aaggacaagg agtcgatcta
    17461 taacctatga gaattatggg attggacgtc ggttcaaaaa cggtaggggt ggcgattagc
    17521 gatccgcttg gttttacagc tcaagggctt gaaatcatcc agataaatga agaacaaggc
    17581 caatttggtt ttgaccgcgt taaggaattg gttgatactt acaaggtgga acgatttgta
    17641 gtgggcttgc ctaaaaacat gaacaataca agtggaccgc gcgtagaagc tagtcaagcc
    17701 tacggagcaa agctagaaga gttttttggt ttaccagtag actatcagga tgaacgcttg
    17761 acaacagtgg ctgctgagcg catgttgatt gaacaagcag atatcagtcg caataagcgc
    17821 aagaaagtca ttgataagtt agcagctcag ctgattttac aaaattattt agatagaaaa
    17881 ttttaatata aaggagaggc tatgtcacac gatcataacc acgaccacga agaacgtgaa
    17941 ctaatcacac tagtagatga gcaaggaaat gaaaccttgt ttgaaatcct tttgacaatt
    18001 gatggaaaag aagaatttgg taaaaactat gttcttctag taccagttaa cgcagaagaa
    18061 gacgaagacg gacaagttga aatccaagct tactcattca ttgaaaatga agatggaaca
    18121 gaaggcgaat tgcaaccaat cccagaagac tcagaagacg aatggaacat gattgaagaa
    18181 gtcttcaaca gctttatgga ggagtaaaaa cgtccggggg acgtttttag ccctgcgcta
    18241 gaaattagaa acgcaggtag tctgggagac tgtatcagcc caactccaga aatggggaag
    18301 agttggaacg tccagtgaac atttttaccc catattcaca ttcatagtgg ctagttatta
    18361 gctaaccagg taagaggtcg ggacgaaaat cctggccttg tttttattag taaccatact
    18421 ttaatgcgat aattgattgg acttttgtca aggagatgtg tatgtttgaa gtagaagaat
    18481 ggctccatag tcggattggt ttgaattttc gatcaggttt gggccgaatg cagcaagcgg
    18541 tggatttgtt aggaaatcct gagcagtctt accctattat ccacgtaaca gggactaatg
    18601 ggaaaggatc taccattgct tttatgaggg aattatttat ggggcatggc aaaaaagttg
    18661 cgacctttac ctcccctcat atcgtctcta tcaatgaccg aatctgcatt aatgggcaac
    18721 ctatagcaga cgcagacttt atccgtttga ctgatcaggt caaggagatg gagaaaacgc
    18781 ttctgcaaac tcctgaccag ttgtcctttt ttgaattgct gaccttggtt gcttttcttt
    18841 attttaggga gcaggaggtg gatttggttt tattagaagt gggaattggt ggcttacttg
    18901 acacgaccaa tgtggtaact ggagagcttg ctgtcatcac ttccattggg cttgaccatc
    18961 aagaaacctt gggtgatagt ctagaagcaa ttgcagagca gaaagctggt attttcaagg
    19021 ctggtaaaaa ggcagtgatt gcgaaattgc ctccagaagc tagacttgtc tgtcagaaaa
    19081 aagccgaatc tttagctgtt aacctttatc aggcaggtca agatttttta atgctgaatg
    19141 gtgatttttc aagctcttta ctaaatcttt cacagctgaa cataggctta gaaggagtct
    19201 atcagcagga gaatgcagcc ttggcgttgc aaacttttct tctttttatg ggagaaggaa
    19261 aggaagctgt tgatgaacag gctgtaagaa aggccttgga acagacccat tgggctggtc
    19321 gcttggagcg tattcgccca cagatttact tggatggtgc tcataacctc cctgccttga
    19381 ctcgcttggt tgagtttatc aaagaaaaag agcaggaagg ctatcgacct caaatcctct
    19441 ttggatcctt gaaacgtaag gattatcaag ggatgttggg ttatctgact gaaaatttac
    19501 ctcaagtgga actcaaaatg accggctttg actatcaagg gtctttggac gagacagatg
    19561 taacaggtta ccatgtaatt ccctcttacc gagaatttat cagcagtttc gaagaaaggg
    19621 cagacgctca agacttgctg ttcgttacag ggtctctcta ttttatctca gaagtacggg
    19681 gctacctgct ggaccgtgag cagataaatt gacccccttt tttgaacttg ttatactagg
    19741 ggaattgctg gttagagaaa atttctctaa attggtagca gaaaggaaat ccatcatgaa
    19801 attaaaaaga ttcacacttt ctcttgcttc tctagcaagt tttagtctct tagtagcttg
    19861 ttcacaaaga gctcaacagg ttcaacagcc tgttgctcag cagcaggtcc aacagcctgc
    19921 tcaacagaat accaatactg caaatgcagg gggtaaccaa aatcaagcgg ctccagtaca
    19981 aaaccaacct gttgctcagc cgaccgatat tgatgggact tatactggtc aggatgacgg
    20041 agaccgtatc actttagtgg taactggaac gactggtaca tggactgagc tcgaatctga
    20101 cggggatcag aaagtcaaac aggttacatt tgattcagca aatcaacgca tgattattgg
    20161 cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc gtcgtagatg atatggatag
    20221 agacccatcg gaccaaatcg ttttaactaa ataagactat gcaagtagaa agggctggat
    20281 ttttcggctc tttgtcaact gtagtgggtt gaagtcagct aagctcgaga aaggacaaat
    20341 tttgtccttt cttttttgat attcagagcg ataaaaatcc gttttttgaa gttttcaaag
    20401 tttcgaaaac caaaggcatt gcgcttgata agtttgatga gattattggt cgcttccagt
    20461 ttggcattag aatagtgtag ttgaagggcg ttgacaatct tttctttatc tttgaggaag
    20521 gttttaaaga cagtctgaaa aataggatga acctgcttaa gattgtcctc aataagtccg
    20581 aaaaatttct ctggttcctt attctggaag tgaaaaagca agagctgata gagctgatag
    20641 tgatgtttca agtctttcga atagctcaaa agcttgttta aaatctcttt attggttaaa
    20701 tgcatacgaa aagtaggacg ataaaattgc ttatcactca gtttacggct atcctgttga
    20761 atgagtttcc agtagcgctt gatagccttg tattcatggg attttcgatg aaactgattc
    20821 atgatttgga cacgcacacg actcatagca cggctaagat gttgtacaat gtgaaagcga
    20881 agctgtttag ccaagccata gtaagggcta aacatatcca tagtaataat tttgacgcga
    20941 catcggacgg ctctatcata tttaagaaag tgatctcgga tgacagcttg tgttcttccc
    21001 tcaagaacag tgatgatatt gagattgtta aaatcttgcg caatgaagct catctttccc
    21061 tttgtaaaag catactcatc
//
LOCUS       DAVMEF010000016        20993 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMEF010000016 DAVMEF010000000
VERSION     DAVMEF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.016"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..3491)
                     /gene="mfd"
                     /locus_tag="VYQ30_000434"
     CDS             complement(<1..3491)
                     /gene="mfd"
                     /locus_tag="VYQ30_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1965598.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFCDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKES"
     gene            complement(3492..4061)
                     /locus_tag="VYQ30_000435"
     CDS             complement(3492..4061)
                     /locus_tag="VYQ30_000435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1965599.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(4132..5247)
                     /gene="ychF"
                     /locus_tag="VYQ30_000436"
     CDS             complement(4132..5247)
                     /gene="ychF"
                     /locus_tag="VYQ30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1965600.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(5367..6533)
                     /locus_tag="VYQ30_000437"
     CDS             complement(5367..6533)
                     /locus_tag="VYQ30_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1965601.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDIIVPRRKAKEEQKLKYLDDDNLKKFLTYLEQLPNTYKNFFDTVLYKTLLATG
                     LRIRECLALEWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSVRMIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(7315..7983)
                     /locus_tag="VYQ30_000438"
     CDS             complement(7315..7983)
                     /locus_tag="VYQ30_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEV1965602.1"
                     /translation="MTYSQNYLDDILVRMAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLDSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGAEFQTASPAETPYLMTQWADNTAYRLEHAQNEKEVLEILADTHIQIERIHPFSD
                     GNGRTGRLILMYLAMKYLNAPIIIPKDSRAHYMELLANQNVTGLADLFKKSLDYERIR
                     KKQF"
     gene            complement(8120..8836)
                     /locus_tag="VYQ30_000439"
     CDS             complement(8120..8836)
                     /locus_tag="VYQ30_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1965603.1"
                     /translation="MADNIKRLLSAKGLNPRQLAIALDFKYTTVNDWVNAKTYPRIDK
                     IEMLANFFNVSKSDLVENKNAETPTTSPIQSIYDQLTPPRQEKALTYLKKQLLEQKNE
                     NIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNMEKCFYTDEIPDDYDAIAYV
                     VGNSMEPKIKNGDYLFIKNTPQVDYNTIGIFQVDGANYVKKLRQGYLESLNPECADIQ
                     LDESNDIRTIGEVVSIYREN"
     gene            9040..9231
                     /locus_tag="VYQ30_000440"
     CDS             9040..9231
                     /locus_tag="VYQ30_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1965604.1"
                     /translation="MRLTLKALRANSNMKQSEVAQKLGISTTTWSKWENGKSFPDVAQ
                     VKEIEKLFGVAYDDIIFLS"
     gene            9350..9535
                     /locus_tag="VYQ30_000441"
     CDS             9350..9535
                     /locus_tag="VYQ30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965605.1"
                     /translation="MNFKEFKTWLDDAVSMAEAMALPENTGVLDDLIENAANNLAFIA
                     ELVENRQLIYRKPRHED"
     gene            9997..10449
                     /locus_tag="VYQ30_000442"
     CDS             9997..10449
                     /locus_tag="VYQ30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965606.1"
                     /translation="MEETAYENLTRRIDRISAEMREIGEKTGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSYKIEAVSKSTTGMFFHPGIFEMDAMRQAFFKRQAMHFFENEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            10446..10646
                     /locus_tag="VYQ30_000443"
     CDS             10446..10646
                     /locus_tag="VYQ30_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965607.1"
                     /translation="MTLDLNNMTQAEFDEVMADIKARNPNIFQLISDFLDRKVTPEEM
                     DEFLKMERAEQVEYIKNYQARA"
     gene            10648..10863
                     /locus_tag="VYQ30_000444"
     CDS             10648..10863
                     /locus_tag="VYQ30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965608.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            10860..11201
                     /locus_tag="VYQ30_000445"
     CDS             10860..11201
                     /locus_tag="VYQ30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1965609.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            11194..11469
                     /locus_tag="VYQ30_000446"
     CDS             11194..11469
                     /locus_tag="VYQ30_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965610.1"
                     /translation="MFSLSRESEHDLTHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            11610..12470
                     /locus_tag="VYQ30_000447"
     CDS             11610..12470
                     /locus_tag="VYQ30_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV1965611.1"
                     /translation="MTIYEARGFQSNLVYPFDKMEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRSFSS
                     IARAEYRKRG"
     gene            12550..14004
                     /locus_tag="VYQ30_000448"
     CDS             12550..14004
                     /locus_tag="VYQ30_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV1965612.1"
                     /translation="MWELYSRIWELGDQWRKENSYIVNEGKKNERVEIPRPSVAIVAK
                     ALQEICHFTFIGEGVISDISKLYLYHLDLGHYVSSNDIFRKLLLKYDSRLTSNKFFLE
                     LISYIRTETKMKPPLDDYRYIPVANGVYNIKTHKLEEFSPNFVITSKIQTEYNPCARK
                     PILDGWFDFDRWLEALAVNDKEVVALLWQVINEAINPNRTRKKMVLMVGDGNNGKGTF
                     QALLENLIGRSNISNLKPDQFGKEFYLGALEGKVCNIGDDISNKYLDEVSDLMSVISG
                     DPVQVNKKSLQPVEARFRLLCIFSGNDLPRARNKTMGWYRRLCIIPFRADFNGQKERH
                     EIKDRFIKNKELLEWVLFKVLNMPDFDSFIEPEAVQKMLSKYKNDNDYIKVWVENFYI
                     PNGWHEINHVPMFIARNKLKEFAEDIGIDKPKLGQFSNAVIALLEKSTPYKYTSKNGT
                     VSPKFYEALDPQGFHRNRINKGLWGIHLEDEDLG"
     gene            14613..14771
                     /locus_tag="VYQ30_000449"
     CDS             14613..14771
                     /locus_tag="VYQ30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEV1965613.1"
                     /translation="MEIQNGFIYFKSYGIIKLLEVPRFGSITLKIQDGEIVSSVESKT
                     TIFKKNTD"
     gene            14810..15454
                     /locus_tag="VYQ30_000450"
     CDS             14810..15454
                     /locus_tag="VYQ30_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965614.1"
                     /translation="MAMPLLFLERLEEKEMPTLQEVKNQMDKVRTQLEVFDRFDEEIK
                     KAEQEVKAIKAKKADLQTFEDFQAINAKEKYIADMKAQRTKLEKERIDSIVADARKIN
                     ASGYLETALEQDETVKRQRQEIKQKSIELLELIANYNENYKNTAKRLADEVRETGIEE
                     LFDRLNTSPEYSGVSKPYIYSGVAGYMGNQHRYLDPSDDLAYFVNRINLFEGEQ"
     gene            15454..15684
                     /locus_tag="VYQ30_000451"
     CDS             15454..15684
                     /locus_tag="VYQ30_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965615.1"
                     /translation="MKDEYKQLFQEIEEEKRQADLLQGLAKNVESISKSYEEEAVTLE
                     EFKAMGYKRRHELFEKDKALYDKLLGKEKRGL"
     gene            15748..16014
                     /locus_tag="VYQ30_000452"
     CDS             15748..16014
                     /locus_tag="VYQ30_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965616.1"
                     /translation="MVRSSKARLEKLEEYAGKMEYATTDECISLYVDCLRFAFQQDEI
                     KIDMDDFIKTPFKDIKLADYKKSKKRMDELREMTGISEFSELEQ"
     gene            16065..16406
                     /locus_tag="VYQ30_000453"
     CDS             16065..16406
                     /locus_tag="VYQ30_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1965617.1"
                     /translation="MANYLMTGNVKKASELSEITRKTAYNYLNDATFKRIYRERRSEQ
                     LKESTTLLQNASVEAVNVLREIMLDKTVSPYARQQSAQSILNMAYKAVEMVEVVEQLE
                     ILEAKILDEGD"
     gene            16393..16644
                     /locus_tag="VYQ30_000454"
     CDS             16393..16644
                     /locus_tag="VYQ30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965618.1"
                     /translation="MKAIKNRLNKLNKLAVVDEVDTVFLTQIDTGHFEVRYRIGGKNP
                     KDIIEGYPTKEEAEKAISEIKNTKGLRIFWDNLALLEDN"
     gene            17201..17620
                     /locus_tag="VYQ30_000455"
     CDS             17201..17620
                     /locus_tag="VYQ30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1965619.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEVLESGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMAISELIDKLANPLERSVLRLFYL
                     NDLVASEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
     gene            complement(18071..18265)
                     /locus_tag="VYQ30_000456"
     CDS             complement(18071..18265)
                     /locus_tag="VYQ30_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1965620.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(18330..19466)
                     /gene="dnaN"
                     /locus_tag="VYQ30_000457"
     CDS             complement(18330..19466)
                     /gene="dnaN"
                     /locus_tag="VYQ30_000457"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1965621.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(19625..20986)
                     /gene="dnaA"
                     /locus_tag="VYQ30_000458"
     CDS             complement(19625..20986)
                     /gene="dnaA"
                     /locus_tag="VYQ30_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1965622.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         6769 a         3754 c         4042 g         6428 t
ORIGIN      
        1 ttagactctt ttatctctaa taaactttct ccaaaaatca gcagaccttc taaaatttca
       61 taatctttct tattttggac atcaaataca agctccatta atcccttatt ctcagcgatg
      121 cctgctttta agttcgttac ggataaagct ttaaaataat cttgagctaa aaacaatcgt
      181 tgagtgactt tttcaaattg aattgtaatt ttattatctt ttctttccac acgttgaaca
      241 aagaccttgt ccaagtatga tttaaccaaa ccaatctcta acagataggc tactacatct
      301 gggtattctc caaaacggtc tatcaactcc tcttgtaact cttcataatt gacacggttg
      361 tcaatttgac gaattttctt gtaaatttca atcttatgtc gttgatcaga aatataagta
      421 tcaggaagat aggcatcaat ttgcaaaatc aactcagcat tccctttggt tcttgtgtta
      481 gcgttagcat taccgtttcg tttagcaata gcttcctcta ataactgcga atacaattca
      541 aaaccaacag aatcaatgaa accagactgg gattttccta aaagatttcc tgctccacga
      601 atcgaaagat ctcgcattgc aatcttaaag ccagagccca attctgtaaa tcctttaatc
      661 gcttctaatc tcttttcaga gacttcactg attgattttt ctggacgata catgagataa
      721 gcataagcaa tacgattact acgaccgact cttcctctta actgatataa ggttgacaag
      781 cccatatggt ccgcattttc aataaataaa gtattagcat ttggaatgtc cacccctgtc
      841 tcaataatag tagtcgtcac caagatatcg tattgtccct caataaagtc taatagagta
      901 ttttccaact ggacttcact cattcgacca tgaacatatc caatcgaagc ctccggaatc
      961 aactcctgta attctgaaac cttctgaaca attgtatcaa ctttgttgta aagatagtaa
     1021 acttgacctc cacgctccat ttcacgcaag acagcatcac gaatgacact atcattcttt
     1081 tccaaaacat aggtctgaac aggatagcga ttagtcggcg gagtttcaat aacagacaaa
     1141 tctctgattc ccagcataga catatggagg gtacgaggga ttggcgtagc ggtcaaggtt
     1201 aggacatcca cttgtttctt cagttctttc aaagtttcct tatgcttgac accaaatcgc
     1261 tgttcctcat caataatcat caagcccaaa tcagcaaaca caacatcttt tgacaaaaca
     1321 cgatgtgttc caatcaaaat atcgacttga ccgtttttca atttttcaag tgttgcagtc
     1381 tgctcttttt tacttctaaa gcgactcaac acatcaacat taactgcaaa attttggaat
     1441 cgttccttaa aattcgtata gtgctgttgc gctaaaaccg tcgtcggaac tagaatgaca
     1501 acctgtttgt gatcattgac tgctttaaag gctgcacgca tagcaacttc agtctttcca
     1561 aaaccaacat ccccaactaa aagtcgatcc attggctgag aagcctgcat atccctcttg
     1621 atttcctcaa tactacgaag ttgatcatcc gtttcaacat aagggaaagc atcatcaaag
     1681 gcatcttgat catcatcatc agctgagaaa gcaaaaccct tcaactgact acgttcagag
     1741 tagagtttga ttaaatcatc agctatatcc tctacctggt tcttaacctt ttgcttggcc
     1801 tttttaaaat gaccgtcatt taatttattg agttttggag ctttaccatc acttgaaata
     1861 tatttggaca gtagatgaat ctgttccacg gggatagaaa tttgatcacc attttggtat
     1921 tggacactga cataatcgcg atgaattccc ttgatttcaa tggtttcaat tcctagatat
     1981 tgaccaatcc catggatatg atggacaaca tagtcccctt tttcaagttc attgtaatct
     2041 tttaatctct ctgcatttga aacatgttgt cttcgaaaac gacgctttaa tttcttttga
     2101 aaaatctcat gttcagttat caataaaatc ttttcatcta caaaatgaaa accatgtctg
     2161 agattaccct ctattaagtt tacagattct ttacagatac ttgtcttatc tctagaatcc
     2221 aatttaatct gatattcctc taacatatcc tccaatgttt tacttcccat tgaattgcta
     2281 gactgcagaa taatggtgta atccattttt ttatatcgtt caatttcttc ttttagaaaa
     2341 gaaaactgat tgaaaaattc ctgcatagga tattgattga attgataaat tttgtcaaat
     2401 ttgagatttc ctaaaccctt ttgaagatta gagaaaaagg tcactggact ttgtttttta
     2461 tagatttgtt caatatcaga aaaatactgc atatcagaaa atgctttact attctgtaat
     2521 tcttctgtaa agtactgcgc taagtctctt tcaaagactt catactgatt catcaatttt
     2581 tgataatcat caaagaatat tggagtatct ttttcaatat aatcaaagac agtccatgtc
     2641 ttatcatagc acaaagataa aaacttccga gagtctgcat gactttgttt ttggtgaaaa
     2701 cttgaaagaa tttcttctag gtatgatttc aaaataggtg ataaagtttt tgaaatttgt
     2761 ttttctaaag ctgactgtcc tcgttgataa tccttttctc tcaaaagcat atcactagct
     2821 ggaaagatag tgagttctgt cttattttct ttcgataatt gtgtttctac ttcaaatgac
     2881 ctgataccat caatttcatc acaaaaaaac tcaattcgac aaggttctaa ctgggatatt
     2941 tcaaaaatat ctaaaatatc tcctcgaaga ctaaattcgc cctgagtttg tacttgagta
     3001 acttttcgat agccattttc ctttaactga tggataaacg cgtgttgatc atattcttca
     3061 ccaactgaga tttttacaat actatctttg aatgcattgg gagacggtaa aatcaatcga
     3121 cttgctgcga tattacaaac taaaatccct ttcttagatg aatcagtcaa aaaacgcaag
     3181 gcttcaaccc gtgaaataat tttttcctgt gaagacatca aaaactccac cataggagca
     3241 tcatctacca aaaatggata gacgagttcc tcacccaaga tagaaataag atcactaaca
     3301 agtccttctg cttctccata agttgacgtc aataacacaa tcctatcttc tttttctaaa
     3361 ctgcttgcaa ttgcaagagc cttagtagat gttgataaac caagtattag ttgtcttttc
     3421 ttatctgtta aattttgatg ccatttttta atctgatcat tttctgagaa taaatctaat
     3481 aaggtcacca tttatccgtt atacctctgc attgttttct caaaattttt ctcttgtaaa
     3541 tagtatttta cagaatcgtc aactttgtca acagactgta aaataccgat ataatcatcc
     3601 ctgtcaaact tactcaaaac atgatgaaca actgacatac catttttagg tcttccaatt
     3661 ccaatcttaa cacggttaaa gacctgagtt cctatatgtt gaataataga cttgatacca
     3721 ttatgaccac ctgctgagcc ttttgctctt aaacgaattt tcccaacttc catgtcaaga
     3781 tcatcgtaaa tgataagtaa atcgtcaata tccaaaccat agtaagttaa taaagcatga
     3841 actgcttttc cactttcatt cataaaggtc gttggtttaa ccagataaat tttttctcca
     3901 tttaggaaaa aggatgctag gtcagcttga aatatcttat cgtgtgtaaa agtgacattc
     3961 tgtttcttcg ctagttgatc aatcaacata aaaccaacat tgtgttttgt ttcaaaatat
     4021 ttatcccctg gatttcccaa gcctacaagt aatttggtca tttatttttc ctttcaaaag
     4081 ccaaaagggt tggaattttt ttccaaccta attgacacca tttattaatt tttagacatt
     4141 aaagcggaat tccatgatat cgccatcttg aacgatatat tcttttcctt cttcacgcaa
     4201 gcgtccagct tcttttacgg ccttttcaga tccgtatttc actagatctt catatgacat
     4261 ggttactgca cgaataaagc ctttttcaaa gtctgagtgg ataataccag ctgcttgagg
     4321 agccttcata ccacgtttga aagtccaagc gcgaacttct ttttcaccag ctgtgaagta
     4381 agttcccaat ccaagcaagt ggtaagctgc acgcgtcaac ttatctacac ctgattctgt
     4441 caaaccaatg gcttcaagaa actctttttt atcttcatca tccaattcag aaatttcttc
     4501 ctcagcacgc gcagaaataa cgactacttc agcattttct gtcgctgcaa attcacgaat
     4561 ttgtttgaca tagtcgatag agtcaggttc tgaaaccaca tcctcgtcca cattagctac
     4621 ataaagaact ggtttagtcg tcaaaaggaa aagacctttg acaacctttt gttcctcatc
     4681 tgtaaattca atggtacgag ctgatttccc gtcttctagg actggtttaa tcttttgaag
     4741 aacattgaat tctgctactg attctttatc tttttgcgta cgtgccatct tttctacacg
     4801 cgcatatcgt ttgttcactg attctaagtc agcaagaatc aattccagat taattgtatc
     4861 aatatctgca agtggatcta caaaggcgtc ttcacgtcct tgctcgcgca ttacattttc
     4921 atcatcaaaa gcacgaacta cgtgaacaat cgcatctact tcacgaatat tggccaagaa
     4981 tttattccct agcccttctc cttttgaagc tccttttaca atccctgcaa tatctgtaaa
     5041 ttcaaatgtt gtgggaactg tctttttagg agttatcatt tcagttagtt tttgtaggcg
     5101 ttcatctgga acttccacca ttccaacatt tggatcaata gtcgcaaatg ggtagtttgc
     5161 tgcctctgct cctgcttttg taattgcatt aaatagtgtt gatttaccaa cgtttggcaa
     5221 accaacgata cctgctgtca aagccatatt tctcattctc cgttttcatt tcaatcccta
     5281 atattataac acaaaaaggg aaaacttgct aaaattcctt gatatagcta tttcccataa
     5341 ctgaaataat aattttatgg acttttttag gaacttttta atttttcaat ggctttttca
     5401 taaaatgaag tagcattttt ctgattgtct ttggataggt ggctataaat atccatggtc
     5461 atagaaagtt ttgcatgacc gagccgtgtt tgtatctctt tgtaaggtag tccagcattg
     5521 agcaagatac tagcatgagt gtggcggaag gcgtggaacg taaaacgagg gcatccagcc
     5581 cgttccaaat gttttactaa cctgaaacta agcattctag cgtctatatg cttatcaaag
     5641 ctattagaga atactttctc ataggtcaac cccatttctt tcccaatttg ggattgtctt
     5701 gctttataga gccgtagcat gagtaccgtc ttattatcca agtcaatcat tctaacgctt
     5761 gatttagtct tagggccagt gatttctttg ataatattta acgttttatt tacgtctagt
     5821 gtgccgtttt ttaggtcaat atcagaccac tctaaggcta gacactcacg aatgcgcaaa
     5881 ccagtagcta aaagtgtctt atatagcacc gtatcaaaga aatttttgta agtgtttggc
     5941 agctgctcca ggtaagtcaa gaattttttt aagttatcat catccagata tttcagtttt
     6001 tgctcttctt ttgccttacg ccgtgggacg ataatatcac gggcagggtt aaaggggata
     6061 gcttgcaaag atacggcata tttaagtatg cgtttgttta gagagtgaag gagcggatat
     6121 tcttggaatc ctttgcctag ttgattatag tcttttgccc attgattgac ttgcttttga
     6181 atgattgggg gcgtcagttt atccagccta tagtttccga aagctggaag tagatattta
     6241 tctattttga atttggtacc cattaaggtc gattttttaa ccgtatggca gtacgtttct
     6301 agccaattct ccaccagttc ggcgtaagtc tttatctcca ccgctttata tactgtagaa
     6361 ccgtcttttt caaagtctat ttgagcctgt agtgctttgg ctttgagttc tctcttggtt
     6421 cgccctgata tagtcgtctt gactttctta cctgttacgg tatcgatacc aagatagacg
     6481 ctagagcgat agactgtaga accgtctttt ttggtgtatt ctgttatttt catggtttta
     6541 ctcctttttc catcagcagg caagcaatta gaaaaggttt tgagtttaaa ccatgcgagg
     6601 ggctacgaga atgcccctat tttcgatttt aagcagtcag acggtaaatt gtaccagaat
     6661 atgaaacaag gcggatatgg ggtttatatg agcttgtttg tgatgggtgt ttttgatata
     6721 atgttttcgc cccctcaatt tagagaaggg aggtgttata tatgctagag ttgttttccc
     6781 tttttctagc tccgttactt gttaacgtac tgtctgagct tttcaagcta tggataaaga
     6841 gacgtagcaa gtagcattta accctttaag agggtagcaa aaaaccccat cgctgtaacg
     6901 gtggggcttt ttagttacat atgctagaag cactgttttt ccctttatgc tttcattcta
     6961 gcatatgagc cccaatattt caagagtttt atttttttga tattgtttag agcgctttaa
     7021 gcgttcctaa aatgccgaag tgttgaaaat cgtctaaatt ataccgcatt atacttcatt
     7081 agagagttat agactgatga gcgtgagaaa tgccgtcagt cactttttaa atgcaagagt
     7141 ggtataataa tagcgtagac taagaggttt gaaagagtcc caactcaaca gtaaccagac
     7201 ctttctgctt ccaatctctc aagatgctac taatcgcctg aatctttccc gttatttcag
     7261 ttcaggcgat attagacaag tcagcagcag ctagcttgtc ttttttttgt tgttttaaaa
     7321 ctgttttttt cgtattcttt cgtagtccag tgactttttg aagaggtcag ctagtcccgt
     7381 tacattttgg tttgcgagga gttccatata atgcgccctt gagtcttttg ggatgatgat
     7441 aggagcgttt agatacttca tagccaagta cataagtatc aggcgaccag tacgaccgtt
     7501 accatcgcta aatgggtgaa tccgttcaat ctggatatgg gtgtctgcta aaatctctaa
     7561 tacttctttt tcattctgag catgctccaa acgataagca gtattatccg cccattgtgt
     7621 catcaagtag ggcgtttctg ctgggctagc cgtttgaaat tccgccccga taatggcatt
     7681 ttgaacttgc ttaaattgtc cgcgatcatg ttgcagccta tccactaata aggcgtgaaa
     7741 atcttgaacc agccccacag taagagcttg atggtttgcc aatgagtcca agagataaga
     7801 aaaggcctgt ttatggtttt caatctcata aaactcacga atacttttgc cgttgcgtgg
     7861 caaggtgctt tctaagataa tgctgacggt ttcgggtaga gagatagtat tcccttcaat
     7921 accgctagaa tggtaggcca tgcgtactag tatatcgtcc agatagtttt gtgagtaggt
     7981 cataattctc ctttctattt gtcacttttg cccatttttg cgctttagtc tgtaaatcgg
     8041 ctttctattc ttcaaatttc cccgtttttg cacaatagac ccatgggatg gggttgctat
     8101 tggcgaatgt ggggattttt taattctctc tatatatact cacgacttcg ccgatggtgc
     8161 gtatatcgtt gctttcgtct agttgaatat cagcacattc agggttaaga ctttctaaat
     8221 acccttgacg cagtttcttg acgtagttag cgccgtctac ttggaagata ccgatagtgt
     8281 tatagtcaac ttgaggtgta ttcttgataa ataggtagtc gccattcttt atctttggct
     8341 ccatagagtt gccgacaaca taagcgatag cgtcgtagtc gtctggtatt tcatcagtat
     8401 aaaagcattt ttccatgttt aggttatcgt cttgggttat cccgtttccg gcggttacaa
     8461 ctccgataac agggagcgtc ttgctttctc tgtagtcgtc cagactgata atgttttctg
     8521 atactatgtt ttcgtttttc tgctccagca gctgcttttt aagataagtc aaggcttttt
     8581 cttgtcttgg tggagtgagt tggtcataga tggattggat gggggaagtg gtaggtgttt
     8641 ctgcgttttt attttctact aagtcagact ttgaaacgtt gaaaaaatta gccagcattt
     8701 cgattttatc tatacgtggg taagttttag catttaccca atcgttgacg gttgtatact
     8761 taaaatctaa tgcaatagct aattgacgag ggtttaaacc ttttgcactt aaaagtcttt
     8821 tgatattgtc cgccattatt tgcttatttc ctaatgacat ggtgcaaccg cctttctata
     8881 attcataatt taattatacg gttaaaacgt agaaaaagca agggaaacaa aaaaaaagaa
     8941 aataaaatca aaaaaagtgt tgacaagtta tcggttttac cgtatactgt cattgtatcc
     9001 gatttaaacg gatatccaaa agaaaggagt gagggctatt tgcgtcttac attgaaagcg
     9061 ttgcgggcta atagcaacat gaaacaatca gaggtagcac aaaaattagg aatttctaca
     9121 actacttgga gtaagtggga aaatggaaaa agttttcctg atgttgccca agtaaaagag
     9181 attgaaaaac ttttcggtgt tgcttatgac gacattattt ttttaagcta atatccgatt
     9241 taaacggata tagaaaggag cgacccaatc gcaatactat attacatcta caagatactc
     9301 cactggtgct ttaccactgg ggattgataa caaaaaaagg agaaaagaaa tgaactttaa
     9361 agaatttaaa acatggctag atgatgccgt gagtatggct gaagctatgg cattacctga
     9421 aaatacaggg gtacttgacg acttgataga aaatgcggcc aataatctgg cttttatcgc
     9481 tgagttagtg gaaaaccgtc aactgattta tagaaaacct agacatgaag attaaaaaag
     9541 acaataaaaa aagtcacttg ctgatagttt agcgaccgag gcaagcgaca agatttaggg
     9601 tatagatatt tttttctata tctcaattat aacaaaaatc aactaatcaa tcaataacaa
     9661 ctaatagcag gcaagcaatt agaaaaggtt ttgaaaagca tagagcgcca actcttaaaa
     9721 ctggtacttt ctcacgcttt caatttggcg agtctgagcg tgaggctagc aattacaaga
     9781 aaaaaactgt ataagaaaca actcaaaaag acccacgctc agaaatttgg cgaccgagag
     9841 catgaggcat gcgacaggaa aaactgtata agaaacaacc attcaaaatc cccacactcg
     9901 ccatcgctca actttgagtg tgaggatatc cagtatagta aaaggcatta aaaagccctt
     9961 agggcaacta cacaacatag aaacagaggt aaaaatatgg aagaaacagc atacgagaat
    10021 ttaactagac gtatagacag aattagcgca gaaatgcgag aaattggcga aaaaactggg
    10081 cttagtagac tttcgttatt ggcaaatcag acaaaattca tcaaagaaaa tttacaccgt
    10141 ttactttgga ttgaacttcc tgaattgaat gacagctata aaatcgaggc agtttctaaa
    10201 agcactacgg gaatgttttt ccaccctggt atttttgaaa tggacgctat gcgacaagca
    10261 ttctttaaac gccaagctat gcactttttt gagaacgaag cagagcaaca ggcgtatata
    10321 gaacatgctg aaaaggagta tttagaggct actataacct taaaagatat tctttttaac
    10381 tctaaaaatg gaactcagag agtaaataaa agttgtctta tagagaagtt tgaggaggca
    10441 atgcaatgac actagaccta aacaacatga cacaagcaga atttgatgaa gtaatggctg
    10501 acatcaaggc aagaaatcca aacatttttc aactgatttc tgatttttta gatagaaagg
    10561 taacacccga agagatggat gagtttttga agatggagcg agctgaacaa gtggaatata
    10621 ttaagaatta tcaagcgagg gcataacatg aatgaactag atataagtaa cacacaggct
    10681 gctgtcttaa tcgtgatttt gattggctta ctgctttatt taaaccaccg agaccgcaaa
    10741 aaaagcgccc aaattgagcg agaaaaccaa cagacgataa aaacacctag cgaggattta
    10801 aaccctgaat atgggcgata tatccagctt gcaggagtca acgtatgggg agggatggaa
    10861 tgagtctatc agagaatgat aaacgagtat taagattaat caaggtaggg gctgagaact
    10921 ccataacagg gtcagaaatt agcctgacaa ccaagctaac agaaagaaca gtacgtgaca
    10981 ttatcaagcg tctagtagtt aagcacaaca tccctattgt aggggttaga tgtggcgttt
    11041 ttagtgggta ctttatccca gcgaataaag gcgagttact agatggcgct aaggcttttt
    11101 acaaccaagt acaggaggag agcaagcgcc tagcggtgtt gatgaatagc gacctagaaa
    11161 gctacaaaga aaccttgaag gaggtgggcg gatatgttta gcttaagccg agaaagtgag
    11221 catgatttga cgcatggcat tctggaggta gtggaaagat acctggaagc gcgtgagaaa
    11281 gtaccgccaa gattgaccaa gttaataaat agagttgaac tcaaagaaga gttaaaaatt
    11341 agtgacaaca cgctgaataa gtgggaaagt cagggtttaa gacgctatca accaccggac
    11401 gatggtgcta gaataatatt ttatttagtg gatgacattc gtaggtttat gggggtggaa
    11461 aatgggtaga tataacacac acccagcgca aggcggaggt cattatcatg acattaaatt
    11521 gtataagcac cgtggcagat ctttggaaca gttccaagaa caaaagctac tgaagaagtt
    11581 taaaaaaaaa cgcaaaaagg ggcgatagta tgacaattta tgaggctaga ggtttccaga
    11641 gtaatcttgt ctatccattt gataagatgg agccgttcca gtacattgaa cgctttaagc
    11701 ctttggtagt ccctgaaagt gctgatccgg aggaatacaa aagaacccag gcgccatatt
    11761 gtttaagtgg taaggttatg ccggagaaaa acgggagtta caaacgaaac aactccagtc
    11821 taatctatcg ggatttgatt ttccttgatt atgacgatat acaggggacg actgaggact
    11881 tcatagaagc cgtttctagc gctttgtttg gctactctta tatcttatac ccgactatca
    11941 aacatagcat agaaaagccc cgttttcgcc ttgtggtgaa atctaacaac gtgatgaacg
    12001 aggcaaccta taagcaggta gtaaaagaga ttgctgacaa gattgggcta ccctttgaca
    12061 tggccagttt aacatggtca caactccaag ggctaccagt aacaacaggc gacccagcaa
    12121 cttatcaaaa gattgtagag catggacttg attatccagt ccccaaggtt gaaccaagag
    12181 cgaagcaagg aactactgag cgttataaac ctagagtaag tggccagagg tcaatgacta
    12241 tgaggattat tgacacgctt ttcaacggat ttggagacga aggagggcgc aacgtagcgc
    12301 tgacacgctt tgtgggatta ctcttcaata agtgggttga ttgtgaccta gagaccgcct
    12361 atgaactgac aaagatagca aatagcgtga cagttgaacc attgcctatt gaagagctag
    12421 accgcagttt tagtagtata gcacgggctg aatatagaaa gagaggttag aaccatagaa
    12481 ctagaagaat tagcaaacct aaaaagtgaa atcttggagg tcagagagca agaacaacct
    12541 cctagaacga tgtgggaact ttatagtagg atttgggagc ttggggatca atggcgtaag
    12601 gaaaactcct atatcgtcaa tgaaggaaag aaaaacgaaa gggtagaaat cccacgacca
    12661 agtgttgcaa tcgtagccaa agcgttacag gaaatttgcc attttacctt tataggtgag
    12721 ggggttataa gtgatattag taagttatat ctttaccatt tggatttagg ccattatgta
    12781 tcaagtaacg acattttccg aaaattgctt ttaaaatatg actcacgttt gacttctaac
    12841 aagttctttt tagaacttat ctcctatatc cgtacagaaa ctaagatgaa accaccgctg
    12901 gacgattatc gatatattcc agtagcgaat ggagtttata atattaaaac tcataaacta
    12961 gaggaattta gtcctaattt tgtcattacc agtaagatac aaacggaata caatccatgc
    13021 gccagaaagc ctattttaga tggttggttt gattttgata gatggttaga ggctttagca
    13081 gttaacgata aagaagttgt agctttattg tggcaagtca taaatgaggc tatcaatccc
    13141 aaccgaacac gtaagaaaat ggtgctaatg gttggagatg gtaataatgg aaaaggtact
    13201 tttcaagcct tgctggagaa tttaatagga cggtcaaata ttagtaattt gaaacctgac
    13261 cagtttggga aagagttcta tttaggcgct ttggaaggaa aggtttgtaa tattggagat
    13321 gatatttcta acaagtacct ggatgaagtt tctgacctta tgagtgtaat tagtggagat
    13381 ccagtgcagg tcaataaaaa aagcttacaa cccgtggaag cacgtttcag actattgtgt
    13441 atcttttcgg gcaatgattt gcctagagct agaaataaga ccatggggtg gtatagacgg
    13501 ctttgtatca ttcctttccg tgcagatttt aacggtcaga aagaacgtca tgaaatcaaa
    13561 gaccgattta taaagaacaa agagctgcta gagtgggttt tgtttaaagt tttaaatatg
    13621 ccagattttg atagctttat agaacctgaa gcggtacaaa aaatgcttag caagtacaaa
    13681 aacgataacg attatatcaa agtttgggta gagaatttct acataccaaa cgggtggcat
    13741 gaaataaacc atgttccaat gtttattgca agaaataaat taaaagagtt tgctgaagat
    13801 attgggatag ataaacctaa acttggacaa tttagcaatg cagtcatagc attattagag
    13861 aaatcaaccc cttataagta cacttcaaag aatggaacgg ttagccctaa gttttatgaa
    13921 gcacttgatc cacaaggttt tcaccgtaat agaattaaca aaggtttatg gggaatacat
    13981 ttagaagatg aagatttagg ttaggtttta aaatactaaa aaatcaataa caacaaggtt
    14041 tgtagcaatt taggttaggt gtgaatttaa aacctaacct gaaacctaac ctttgtaaac
    14101 gttgatataa aagtctttgc gagtatttag gttaggaggt taggttttat ttctaatatc
    14161 tctatataaa aaaataaata taaacgtgtt atataggcgc gaacttgaaa agtaaaccta
    14221 accctaacct aaacgcttaa aaacgttgat atgaaaggat ttttgtgggt taggtttaaa
    14281 cctaatcaaa acctaaccta agagaaaaaa ggagcaaacc agatggcgat gaacatttgt
    14341 caaagagtga ttagactaga agaaaccgtt gagcggatca gtgaggcgct ggatggcttt
    14401 attgagaatg cgaaccataa cgctatagta agcaataaaa aacatcgaac ggattcagga
    14461 aggttttgaa atagtcttta aagagcttga tgaactaaaa acagtacctg ataaacacta
    14521 aaacatttaa acaaaatggg gtagatagta ccgaccccct tgcaatggtg taactactag
    14581 tgacaccctt aaaacagatg aaaggataac aaatggagat acaaaatggc tttatttatt
    14641 tcaaaagtta tggtataata aaactgttag aggttccaag gtttgggagt ataacgctta
    14701 aaatacaaga cggcgagatt gtctccagcg tggaatctaa aacaacaata tttaaaaaga
    14761 atactgactg aaaaaatcag aggtatagca taaagtagag taatctattt tggctatgcc
    14821 tcttttgttt ttggaaagat tggaggaaaa agaaatgcca acattgcaag aagtcaaaaa
    14881 ccagatggac aaagtacgga ctcaattaga agtttttgat cgctttgatg aggaaatcaa
    14941 gaaagcagag caagaagtta aggctattaa agctaagaaa gcggacttac aaacttttga
    15001 agattttcaa gctataaacg caaaagaaaa gtatattgct gatatgaaag cacaaagaac
    15061 taaactcgaa aaagaacgaa ttgactcaat cgtggcagac gctagaaaaa tcaatgcctc
    15121 aggttattta gaaacagcat tagagcaaga tgaaacagtg aaacgccaac gtcaagaaat
    15181 caaacagaaa tctattgagc ttctggagtt gattgcaaat tataacgaaa actacaaaaa
    15241 tactgctaaa agattggcgg atgaggtaag agaaacaggt atcgaagagt tatttgaccg
    15301 cctgaatacg tcaccagaat atagtggagt aagtaagcct tacatttaca gtggtgtagc
    15361 tggttatatg ggaaaccaac accgctattt agatccgagt gatgatttag cgtattttgt
    15421 gaatcgtatc aatttatttg agggagaaca ataatgaaag atgaatataa acaactattt
    15481 caagaaatcg aagaagagaa gagacaagct gacttactgc aaggacttgc caagaacgta
    15541 gaaagtattt caaaatctta tgaagaggaa gctgtgaccc ttgaagagtt taaagctatg
    15601 ggctacaagc gtagacatga actatttgag aaagataaag ccctgtatga taagctacta
    15661 ggcaaggaaa aacgcggttt gtgatgtgga ggggagcaat cccctttttt tacataaatc
    15721 agacagaatt acacacgagg taggggaatg gttagaagtt caaaagcaag actagaaaag
    15781 ttagaagagt atgctggaaa gatggaatat gcaacaactg atgaatgtat atctttatat
    15841 gtggattgtt taagatttgc ttttcagcaa gatgaaataa agatagatat ggatgatttt
    15901 attaaaacac catttaaaga tattaagcta gcagactata agaaatcaaa aaaacgtatg
    15961 gatgaactta gggaaatgac tgggataagt gagttttcag aattagagca ataaagcgag
    16021 gtgaacagta tttgacagga ttaagcagaa aaaaagaaat atttatggcc aattatctca
    16081 tgactgggaa tgtaaaaaaa gctagtgaat taagtgagat taccagaaag acggcttaca
    16141 attacttgaa cgatgcaacc tttaagagaa tttaccgtga aagacgtagc gaacaactta
    16201 aggagtctac tacgctgtta cagaatgcaa gcgttgaggc agtcaatgta ttaagggaaa
    16261 tcatgcttga taagacagtg agcccgtatg cgagacaaca aagcgcccaa agcattctaa
    16321 acatggctta taaggcggtt gaaatggttg aggtagtgga gcaattagaa atattagagg
    16381 cgaagatact agatgaaggc gattaaaaac cgtttaaata agttgaataa gttagcggtg
    16441 gttgatgaag ttgataccgt gtttttgacg cagattgata ctggacattt tgaggtaaga
    16501 tataggatag gtggaaaaaa tcccaaagat attatagaag gctatccgac aaaagaagaa
    16561 gcagaaaaag ctatttcaga aattaaaaat acaaaaggat tgcggatatt ctgggacaat
    16621 ttggctttat tggaggataa ttagagctta tcatttatca agcagataag agaaataata
    16681 accctcctta actccttgag aaacgacaag cagataagcg aagtttttag ccctcttatt
    16741 tcggatttac tccttgcaga atgacaagca gataaggacg acaataagcc ttcttaattg
    16801 attacaaaac gatagggggt taagacgaat ttaaaagctc cttattttcg tttttctcct
    16861 tgtcaaacgg taaggaaata agaactaaaa taactcccct tattttccta ccaaatgata
    16921 agcagataag caaagaaata acttgcctta attgcttgca gaatgacaag gaaataaggg
    16981 agataaaaac atcccttaat tgcttatcaa accacaagga gatacggggg ctatctccct
    17041 ccccacgcac ttctatgagc ttcacaccgt cattgtacat tttttctcac ggggaagcat
    17101 ttgaagctag agcgaacata gagaaaacga aaaaaagcca aggcactccg cctcagctat
    17161 aatctcaata atattattat accacaaagg agagcaaggc atgacaccag agcaggtaaa
    17221 agaaaaacta gagggcgtta agtggataaa caaggagatc aaaggattat atttggaatt
    17281 ggaagtcctg gagagtggta ttatcaaaaa gccaacacta agccatagca gggtgcagac
    17341 aagcagagag aacaagacag agaacaatct tataagtgtt ctgaagctaa aagaggatac
    17401 actccagaga attgagcgcc ttaccgagga aagaatggca atatctgagc taatcgataa
    17461 actggccaat ccgcttgagc gttctgttct aagacttttt tacttgaatg atcttgtagc
    17521 ttcggaggtg gctgaagaaa tagatagatc aaatactaca gtatacatta taaggcaaaa
    17581 agctatagaa cacttaacag gtattgtaaa cggggattga tttaacctta ccacggaatt
    17641 aggagagtat ggggaactct aagccccata tttgacccta tttcgcttgg ttttgtaccg
    17701 gacgattaaa acaccatgga taaactagaa gcccttagaa acgaatcagg gggacttttg
    17761 tgaggtcata atcttataaa ccacgcgctt catggtataa tatacctatc agcaaccagc
    17821 aataaaaaaa gcacgtttga acgtgctagt ttcttgcctg ctgaactcat cgatttagta
    17881 gcccttttgt ggggcttttt ttgtggactt tttgagaatt tttaagaact tttaatgatt
    17941 tctaatcaaa tgagattttt caaaaagtca gtaatatcaa gggtttaggc gctataaaca
    18001 agtttaaaag cgtgtagatt acaatctaac acaaaaaggg ataaagtgct aaccctctaa
    18061 ctaagggttc tcagtcaata attttattca tttttcgctc aaaatcatat cgccccatca
    18121 tgacaacatg ctcacaatta ctgcatttta ttttgatatc tgctcctaca cgtgtaattt
    18181 cccaacgatt agccttttta ccagtcgact tgattgtaca agcgtgtgat tttttcatct
    18241 caacaaaatt tccaacttga tacatactac tctccttttc tttttcgatt atatcataaa
    18301 agaggcgagc caggctcaac ctctttcact taatttgtac gaactggtgt aatgagctgc
    18361 atgaagtctt cgtcagtatc tgctggcaca agagtaaatg gacgaacagc tgagataaag
    18421 ctaatggtca ccttttcgct atttaaagct ttaagagaat caatcaagta agttgggttg
    18481 aaactaatgg tcaaatcttc accagtaacc tgatcagtat cgatttcttc gtttacttta
    18541 ccaacttctg gagagtgaac atgggcgcta acaaccccat ccttaatttc aagtttcaca
    18601 gtaccatttt gagtcgcact tgataaaaga cgggcacgct ccattgactg gcgtaagttt
    18661 accacattaa aagtaatagt agtgttaaag tctgttggaa tcaagcgatc tgtatcagga
    18721 tagtttcctt ctaggagacg agtatagaag ctaatatttt cgcttctaaa gaggatttgg
    18781 ttattggcaa agaaaatctc tacagtttcg atatcatctg taaataccgc tgaaaattcg
    18841 cgtagagaac ggctaggaat tacgacatca aaatcatcac tatttttttc aagagtcaat
    18901 tttttctggc ttaggcgatg agagtctgtt gcaactgttt ttaactcttt gtgttgactc
    18961 aatacgaagt ggacacctgt taaaatcgga cgactctctt gtgtacttgc agcaaaggct
    19021 gtttcattaa taattttctt gagtaatttt gtttcaagta ttaaaggagt gcttgctgaa
    19081 atttcttgga ttcgtggata ttgttcgcta tcttttcctt ttagggtaat ttctgatttg
    19141 ccactggtta aaacaatttg attttgttca atttctttaa aatcaagagt cacatcaggt
    19201 aaactagata ctacattgat aaagaaagaa gcttcaagaa ggatcgaacc taaagaagta
    19261 attaacaaac cagcatcttc atttttttga gaaataaaat tttcaattga aatttgacca
    19321 tttgaaccaa ttaaagtaat accttcattg gtcacgtcaa tttttactgt tgataaaata
    19381 ggaatggcat ttttagaact aatagctctc ttagtagtat ttaatgcttg tagaaataaa
    19441 tttttattaa ttgaaaaatg aatcatggat tctcctttat ttatttttag tattagaaag
    19501 ataatagtaa taatagagtc tgtgaaatct gtggaaaaca gcctttaaac aagtcatatc
    19561 aagtttttta gcttgtttaa aaaatgtgga taaaaaagat aaaaaactaa aagttatcca
    19621 caaattattt gattttcttt ttgattgatt caatttctaa acgtaaatta tcgtcttgat
    19681 caatcaaaga ttttattttg gcatgggcat gaatgactgt ggtatgatct tttcccccaa
    19741 attccttccc aatttttgga agactattat ctgttagttc tctagataaa tacatggcta
    19801 cttgacgggc caaaacaata ttttgaaggc gtctacttcc cttcatttct ttgatactaa
    19861 caccataaaa gttaccaact tcagtttgga ttttatcaat tgggatgacg agcatttggc
    19921 taacatcttg tttgcgggct ctaatggctt ctgcagcaat atcaatagtg atatccttga
    19981 ttttttttac tctggcaatt aaagtgatgt cgttgatggc tccctcaaga tctcgaacat
    20041 ttgaatcaaa ttgcccagct aggtattcta gagtatcact ttggaaattg tagcctaaat
    20101 gttccgtctt actttgtaaa atggcaatac gtgtttcaaa gtcagggggt gtgatagttt
    20161 gtgtcaatcc ccaactaaaa cgcgtgacaa gcctctcctc gagcccttct agatgttttg
    20221 gactacgatc actcgttagg acaatctgtt tttgcttgtc atgaagggcg ttaaaggtat
    20281 tgaaaaattc ttcctgagtt gcgacttttt ttccgctgag tgactgtata tcatcgatta
    20341 acaaaagatc aagactacga taggtctttt taaacttttc catttcccca agtcttaggt
    20401 gatcaagaaa gtcattaata aagctttcgg cagggatata tttaacacgc gcattaggaa
    20461 tattttttag aatttcattt ccaatagcgt ttaataagtg agtcttacca aggcctggtc
    20521 ctccatagat aaaaagaggg ttataggtca gagccaaatc ttcagagaca gctaaagcgg
    20581 ctgatacagc ccaaacattt ccatcccctt gaataaagtt atcaaaggta tacttttctt
    20641 ttaatcccgt atctgaataa ggaatagata ctaactttgg actatagtca taaagagtta
    20701 aatttgtagc ttcttcaact tgtgagctag tcgtatcttg aggtttggtg aaaatatagt
    20761 ggggagttat ttcagcgtca taaatttcaa aaccagctac tacaataata tcttttagtt
    20821 gtttttccca gaccatttcc atttcagagc gaggtagaaa tatagtggca acattttcct
    20881 ctaccttgat gagttcagct tgaatagcat agaaatcata catggatcga gtcagtcttt
    20941 cttgtgcaaa ttctaatata cgattccaaa attgtttttc tttcaatcct ttc
//
LOCUS       DAVMEF010000017        20846 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMEF010000017 DAVMEF010000000
VERSION     DAVMEF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.017"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..279
                     /locus_tag="VYQ30_000459"
     CDS             1..279
                     /locus_tag="VYQ30_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965623.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            281..2344
                     /locus_tag="VYQ30_000460"
     CDS             281..2344
                     /locus_tag="VYQ30_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1965624.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRLLQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLTRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(2422..3678)
                     /locus_tag="VYQ30_000461"
     CDS             complement(2422..3678)
                     /locus_tag="VYQ30_000461"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1965625.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            3820..6285
                     /locus_tag="VYQ30_000462"
     CDS             3820..6285
                     /locus_tag="VYQ30_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1965626.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGHLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEITEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            6350..7261
                     /locus_tag="VYQ30_000463"
     CDS             6350..7261
                     /locus_tag="VYQ30_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1965627.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            7491..8189
                     /gene="dapD"
                     /locus_tag="VYQ30_000464"
     CDS             7491..8189
                     /gene="dapD"
                     /locus_tag="VYQ30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1965628.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            8257..9387
                     /locus_tag="VYQ30_000465"
     CDS             8257..9387
                     /locus_tag="VYQ30_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1965629.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLLFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVVASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            9399..9938
                     /locus_tag="VYQ30_000466"
     CDS             9399..9938
                     /locus_tag="VYQ30_000466"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1965630.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRTLSTVYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            9922..10599
                     /locus_tag="VYQ30_000467"
     CDS             9922..10599
                     /locus_tag="VYQ30_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1965631.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQIWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(10666..11565)
                     /gene="galU"
                     /locus_tag="VYQ30_000468"
     CDS             complement(10666..11565)
                     /gene="galU"
                     /locus_tag="VYQ30_000468"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1965632.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(11587..12603)
                     /locus_tag="VYQ30_000469"
     CDS             complement(11587..12603)
                     /locus_tag="VYQ30_000469"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1965633.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            12815..13195
                     /locus_tag="VYQ30_000470"
     CDS             12815..13195
                     /locus_tag="VYQ30_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1965634.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(13377..13793)
                     /locus_tag="VYQ30_000471"
     CDS             complement(13377..13793)
                     /locus_tag="VYQ30_000471"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1965635.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(13861..14511)
                     /gene="phoU"
                     /locus_tag="VYQ30_000472"
     CDS             complement(13861..14511)
                     /gene="phoU"
                     /locus_tag="VYQ30_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1965636.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(14526..15278)
                     /gene="pstB"
                     /locus_tag="VYQ30_000473"
     CDS             complement(14526..15278)
                     /gene="pstB"
                     /locus_tag="VYQ30_000473"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1965637.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(15280..16095)
                     /gene="pstA"
                     /locus_tag="VYQ30_000474"
     CDS             complement(15280..16095)
                     /gene="pstA"
                     /locus_tag="VYQ30_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1965638.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(16088..16951)
                     /gene="pstC"
                     /locus_tag="VYQ30_000475"
     CDS             complement(16088..16951)
                     /gene="pstC"
                     /locus_tag="VYQ30_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1965639.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(17069..17944)
                     /locus_tag="VYQ30_000476"
     CDS             complement(17069..17944)
                     /locus_tag="VYQ30_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV1965640.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGKYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDTIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(18111..19442)
                     /locus_tag="VYQ30_000477"
     CDS             complement(18111..19442)
                     /locus_tag="VYQ30_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1965641.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(19443..20150)
                     /locus_tag="VYQ30_000478"
     CDS             complement(19443..20150)
                     /locus_tag="VYQ30_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1965642.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(20234..20719)
                     /locus_tag="VYQ30_000479"
     CDS             complement(20234..20719)
                     /locus_tag="VYQ30_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1965643.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            20705..20846
                     /locus_tag="VYQ30_000480"
                     /pseudo
     CDS             20705..20846
                     /locus_tag="VYQ30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         6030 a         4034 c         4515 g         6267 t
ORIGIN      
        1 atgttaaaag aagtattaac cgttgcaaaa gttgcgaaaa aatcttcatt atttttgggt
       61 ggtgtcgcat ttggtaccct tggtttgaaa atcttagcaa gtaaggaagc taaaaaaggt
      121 tattctaaag ctttggctaa ggcttacaag ttgaaagacg agctagatgc atctgtttct
      181 gttgtgaagc aacatggaga cgatgtcttg caagatgcca aatatttgta cgagcaagag
      241 aaaaaagaag agcaattaga tagccttata ggtgaataat atgtctttta aagtgctaca
      301 tagaggatac caacatatcc gactatcatc ttctttttca cttaccttgg atattcaaga
      361 ctatcttcgt tccttggcga gagatgaaaa ggggattgag tctatccagt tttacatgga
      421 tcaacagcac tttactctac gcataaaaga aggcttttct gtattagata atgcagaagc
      481 ctttttaaaa agaattgata aagggaaagt ttctgagttg atgactcttc ccattcgtag
      541 agaagagagt gcttattcta ttgtttcagg tgcagcggtt aagcgtgtac tttttcgtag
      601 ttttgtgccg tatcctattc gctatatatg gacttgttat caggctttgg gttatattag
      661 agaagcctat caaacactag cgcgtaagga actaacgatg gaagtcttgg actgttcggc
      721 tattttattg tctttgttta tgaaccaatc caagacagct agcaatatca tgtttatgct
      781 tgatttgggg aatcatttag atcagtggtc cttgaaaaaa actgcaacag atttagaaca
      841 gagtcttctt gcaaaagaga gcgatgtatt cctagtacag ggcgatacgg ttgttagtat
      901 caagagttcc gatgttcaaa taggagatgt cttgatctta tctcaaggaa atgaaattct
      961 gtttgatgga caagtagttt caggtttagg tatggtcaac gaaagttcct tgacaggaga
     1021 gagttttcca gttgaaaaaa gagagtctga tttggtttgt gcaaatacag tattagaaac
     1081 tggagagtta cgcatttgtg taacagataa tcagatgaac agccgtcttt tacagctgat
     1141 tgagttgatg aagaaatctg aagaaaacaa gaaaacgaaa caacgctatt tcatcaagat
     1201 ggcggataaa gtcgtcaaat ataatttctt gggggctggg ctgacttacc tattgacagg
     1261 ttctttttct aaggctattt ctttcctatt ggtcgatttc tcctgcgctt tgaaaatctc
     1321 tactcctgta gcttatttga cagttatcaa ggaagggttg aaccgtgaaa tggtgattaa
     1381 ggatggagat gttctggaga aatatctgga agttgatact ttcttgtttg ataagacagg
     1441 aacaatcaca actagttatc ctatagttga aaaggtgtta ccttttggag actatagtga
     1501 ggaagatatt ctcagaatca gtgcctgtct tgaggaacac atttatcatc ctattgctaa
     1561 tgccatcgtc aagcaagctg agatagaggg aattgaacat gaggaaatgc atgggaaact
     1621 ccaatatatc gcaagcaagg ggatcaaatc tcatatagat ggccaaccag ttcttattgg
     1681 gaattatgtc ttgatgcagg atgagcagat tcatatcagt tcagaacaaa atgctttaat
     1741 tgaagagtac aagagtcact acaatctctt attcttggct tatcaaaatg aattgattgg
     1801 aatgttctgc attcatactc ctttgagaaa agaagcaaaa acagccttgg ataaacttaa
     1861 ggcacagggg aaaaaattga ttctggcaac aggagacacc ttgactagga cagaggaatt
     1921 agtcaaagat ttgccctttg atcaggtcta tacagacttg aaacctgatg ggaaatttga
     1981 gttagtagag aaactgcaga aagcaggtca cactattttg atggttggag atggattgaa
     2041 tgactcagcg gctctaactc tatcagatat cggtgtggtg atgaatgaga gtgcagatat
     2101 ttctaagcag atgagtgata tcttattgtt agataatcgt ttggatttct tccaagagtt
     2161 ggattcgtta tcatcatctt tgcaaacact catcaagaaa aatattcaag ataccgttgt
     2221 cgtaaatagt agtttgattg gctttggctt atttaattgg ctcagtcctt caaatctctc
     2281 tatcttacat aatctaacaa ccttgcgcat tgtcctgcgt agcctgtcta ttaaaaatag
     2341 atagatgaga gttattaaca gcaaaaagga gttacctttc tcgaggttaa ctcctttgtt
     2401 tatagataaa tgttgaatag tttagtaagt caatacaaag tattttttct tcccacgacg
     2461 gataacagtc agttcatttt ctaacttatc agcgtcactc aagacatagt caaggtcttg
     2521 gatgcggtcg ccgtttacgt agatggctcc gttttggacg tcttcacggg cttggcgttt
     2581 tgagttaact ataccagatg agacgagcag ttccacgata ttgttgtgtt cgtctgcctg
     2641 tacttggtag tttggcacac cacgaagtcc ttgtttgagc tctttgacag aaaggttttt
     2701 gatgtttcct gcaaagagtt gctcagtgat gttaagtgct tctttgtagg cttcttctcc
     2761 gtgaacaagt gtaacaactt cacgagccaa gactttttga gccaagcgtt cgtgtggcgc
     2821 tgcttcaaat tgtttgcgaa tatcttcaat ctcatcaagt gacaagaaag taaagatttt
     2881 caagaagcga acagcgtcag cgtccatcac gttcatccag aattggtaca tttcgtatgg
     2941 agaagtcttt tcgggattga gccagacggc atttccttct gatttaccaa atttcttacc
     3001 agttgcatct gtgattagtg gaacagtgat aacgtgacca gtcttgtccg ccttacgacg
     3061 aagcaattcg gtaccagctg tcatatttcc ccactggtca gaaccaccga tttgaagagt
     3121 gacattatgg tcttggttaa ggacgaagaa gtcgtaccct tgcatgattt ggtaagcgaa
     3181 ctcagtgtaa gaaattcctg tttcgatccg ttttttaaca gattccttac tcatcatgta
     3241 gttgaccgtg aagtattttc caatatcacg gaggaagtca atgaagctga tgctgccaaa
     3301 ccagtcgtag ttgttgacca tgacagcctt attttcgcca ttttcaaagt caagaaaacg
     3361 agaaagttgt ccttggatag acttgaccca gccatctact gtgtcttttg tttggagact
     3421 acgttcagca tctttgaagg acggatctcc gatgagacct gtagcaccgc caacgagcgc
     3481 ataaggtttg tgacctgcta gttgcaagcg acgacttgtc aagattgcga caaggtggcc
     3541 taggtgaagg ctgtcagcag ttggatcgta gccagtataa taagaaactt gaccttcttc
     3601 tagggcttta cgcaaagctt cttcatcagt cgtttgaaat atcaaaccac gctcttttag
     3661 ctcatcaaaa atgtgcatgt gtcttttctc ctttttaaaa ttattgtttc tacctattgt
     3721 atcacaaact tgggcaataa cctagcagaa tagggaagaa agtggctatt ttattgaaaa
     3781 tttccctttt gtggtataat agatagagtg aggaaatcca tgcaaaatca attaaatgaa
     3841 ttaaaacgaa aaatgctgga atttttccag caaaaacaaa aaaataaaaa atcagctaga
     3901 cctggcaaga aaggttcaag taccaaaaaa tctaaaacct tagataagtc agccattttc
     3961 ccagctattt tactgagtat aaaagcctta tttaacttac tctttgtact cggttttcta
     4021 ggaggaatgt tgggagctgg gattgctttg gggtacggag tggccttatt tgacaaggtt
     4081 cgggtgcctc agacagaaga attggtgaat caggtcaagg acatctcttc tatttcagag
     4141 attacctatt cggacgggac ggtgattgct tccatagaga gtgatttgtt gcgcacttct
     4201 atctcatctg agcaaatttc ggaaaatctg aagaaggcta tcattgcgac agaagatgaa
     4261 cactttaaag aacataaggg tgtagtaccc aaggcggtga ttcgtgcgac cttggggaaa
     4321 tttgtaggtt tgggttcctc tagtgggggt tcaaccttga cccagcaact aattaaacag
     4381 caggtggttg gggatgcgcc gaccttggct cgtaaggcgg cagagattgt ggatgctctt
     4441 gccttggaac gcgccatgaa taaagatgag attttaacga cctatctcaa tgtggctccc
     4501 tttggccgaa ataataaggg acagaatatt gcaggggctc ggcaagcagc tgagggaatt
     4561 ttcggtgtag atgccagtca gttgactgtt cctcaagcag catttttagc aggacttcca
     4621 cagagtccca ttacttactc tccttatgaa aatactgggg agttgaagag tgatgaagac
     4681 ctagaaattg gcttaagacg ggctaaggca gttctttaca gtatgtatcg tacaggtgca
     4741 ttaagcaaag acgagtattc tcagtacaag gattatgacc ttaaacagga ctttttacca
     4801 tcgggcacgg ttacaggaat ttcacgagac tatttatact ttacaacttt ggcagaagct
     4861 caagaacgta tgtatgacta tctagctcag agagacaatg tctccgctaa ggagttgaaa
     4921 aatgaggcaa ctcagaagtt ttatcgagat ttggcagcca aggaaattga aaatggtggt
     4981 tataagatta ctactaccat agatcagaaa attcattctg ccatgcaaag tgcggttgct
     5041 gattatggcc atcttttaga cgatggaaca ggtcgtgtag aagtagggaa tgtcttgatg
     5101 gataaccaaa caggtgctat tctaggcttt gtaggtggtc gtaattatca agaaaatcaa
     5161 aataatcatg cctttgatac caaacgttcg ccagcttcta ctaccaagcc cttgctggcc
     5221 tacggtattg ctattgacca gggcttgatg ggaagtgaaa cgattctatc taactatcca
     5281 acaaactttg ctaatggcaa tccgattatg tatgctaata gcaagggaac aggaatgatg
     5341 accttgggag aagctctgaa ctattcatgg aatatccctg cttactggac ctatcgtatg
     5401 ctccgtgaaa agggtgttga tgtcaagggt tatatggaaa agatgggtta cgagattact
     5461 gagtacggta ttgagagctt gccaatgggt ggtggtattg aagtcacagt tgcccagcat
     5521 accaatggct atcagacctt agctaataat ggagtttatc atcagaagca tgtgatttca
     5581 aagattgaag cagcagatgg tagagtggtg tatgagtatc aggataaacc ggttcaagtc
     5641 tattcaaaag ctactgcgac gattatgcag ggcttgctac gagaagttct atcctctcgt
     5701 gtgacaacaa ccttcaagtc taacctgact tctttaaatc ctactctggc taatgcagat
     5761 tggattggga agactggtac aaccaaccaa gacgaaaata tgtggctcat gctttcgaca
     5821 cctagattaa ccctaggtgg ctggattggg catgatgata atcattcatt gtcacgtaga
     5881 gcaggttatt ctaataactc taattacatg gctcatctgg taaatgcgat tcagcaagct
     5941 tccccaagca tttgggggaa cgagcgcttt gctttagatc ctagtgtagt gaaatcggaa
     6001 gtcttgaaat caacaggtca aaaaccaggg aaggtttctg ttgaaggaaa agaagtagag
     6061 gtcacaggtt cgactgttac cagctattgg gctaataagt caggagcgcc agcgacaagt
     6121 tatcgctttg ctattggcgg aagtgatgcg gattatcaga atgcttggtc tagtattgtg
     6181 gggagtctac caactccatc cagctccagc agttcaagta gtagttctag cgatagcagt
     6241 aactcaagta ctacacgacc ttcttcttca agggcgagac gataattctc taaatgaagt
     6301 ggccaatcaa tggattgcca cttttttcct ttcgtgttac aataagggta tgaatcagta
     6361 tcagaaaaag attgttaacg gaaaaattta ttcgctccta tccggcttaa tatggggaat
     6421 ctgtggaatt ttaggagagt acttctttac tcattatcag gtgtcttcgg gctggattac
     6481 ctctatgcgt ttgacactgg cagggagtct tgtactcatt tggtctgcaa tacaattaaa
     6541 atcgcaagtg ctagatattt ggcgagacaa gaaaaattac ctgccctttt tagcctatgc
     6601 tattttgggg attttttcag tccagtattt tttctatctc tgtgtagaat actcaaatgc
     6661 tacgacagca actattttac agtttattag ccctgtcttt atcctctttt acaatcgctt
     6721 ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat gttttggttg ccatgctggg
     6781 tgtttgcttg atggcgacaa agggagatct ctctcagtta tccatgacgc cgctagctct
     6841 tataacaggt ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc cccaaccttt
     6901 tgctaagcgt tatggttttg ttcctacggt tgggtggggg atgattttgg caggtttgtt
     6961 tagcaatgtc ctctcgccgg tttatcagct ttcctttact cttgatattt ggagtatctt
     7021 gatttgcctc attatcgctt tctttggaac ggcttttgct tttttcattt ccatgaaggc
     7081 tgtgtccttg gtttctcctt tggtggtttc cgttatcagt gccagtgaac ctctctcttc
     7141 tgctctcttg agtgttttgt tcttaggatt agtagtggat tggtccctcc ttctagctat
     7201 agccttgatt attttaccca tgatattttt gtctatagaa gaagcgaaag aaagtagata
     7261 aaaagtcttt tcttaattct aaaagtgtgc tatactagaa tagtcaataa aacacgggga
     7321 gatagttgtg aagagtgttt ttaggttgta tcggtagggg cttgctttta ccgacagcac
     7381 gttttatctc acatccctaa aaaatcatac ctaaaaacag acaaaaaggt ttcaaatttg
     7441 aggccttttt tggtagaata ggtatcatta taacgaacca ggaggcacct atgactgcta
     7501 caaaaatgaa cgctcaagaa attatccaat ttatcgccaa tgctgaaaag aaaaccagtg
     7561 tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt gtcaaactag
     7621 gaaatgtcct attcggagac tggaaggacg tggctccgct tcttgaaggt ttggtagaaa
     7681 atcaagatta tgttgtcgag caagatgctc gtaattctgc agttcctttg ctagataagc
     7741 gtgctatcaa cgctcgtatc gagccaggtg ctattatccg tgaccaggtg gaaattggtg
     7801 acaatgctgt tatcatgatg ggagctgtta tcaatatcgg tgctgaaatc ggtgctggaa
     7861 ccatgattga catgggtgcc atccttggtg gccgtgccat cgttggaaaa aatagccacg
     7921 ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc tagtgctgag ccagtccgtg
     7981 tcggagacaa tgttcttatc ggtgctaatg cagtggttat cgaaggagtc caaatcggta
     8041 gtggttcagt tgtcgcagca ggagctattg ttacccaaga tgtcccagaa aacgtggtag
     8101 tagcaggtgt tccagctcgt attatcaaag aaattgatgc ccaaactcaa caaaaaacag
     8161 cgctagagga tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc ggaacccttt
     8221 ttccagcctc tttctgctat atcggaggac agatagatgt tagatttgat tcagactaga
     8281 cgagatttac accagattcc agagattggc ttggaggagt tcaagactca ggcttatttg
     8341 ctggatgtga ttgagaaatt gactacgggc aaggattttg ttcaaattcg tacttggcgg
     8401 acagggattt tggtctacct gcagggaagt cagccggagc gaaccattgg ttggcggaca
     8461 gatattgatg gcctgcctat cgtcgaacaa acaggtctac tttttgcttc tcaacaccaa
     8521 ggtcgcatgc atgcctgtgg tcatgatttt catatgacca ttgccttagg ctgtcttgag
     8581 cgcgctcttg aggagcaacc caagaataat ctgctctttc tatttcagcc tgctgaagaa
     8641 aatgaagctg gtggtatgct catgtatgag gacggtgctt ttggagattg gttgccagat
     8701 caattctatg gtctccatgt tcgtccagat ttgaaagtcg gccagattgc gactaatact
     8761 catacactct ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggcaa aggtggccac
     8821 gcagcttttc ctcatgaagc caatgacgcc ttggtggtgg ctagttactt tgtgacccaa
     8881 gtgcagtcag ttgttagccg caatgtcaac ccaatcgagg gagcagtggt gaccttcggc
     8941 gtttttcaag ctggaaccac caacaatgtc atcacagaca cagctttttt acatggaact
     9001 attcgtgcct tgacacagga catgagtctc ttggtgcaaa agagggtaaa aacagttgca
     9061 gaaggggttg cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca aggtggatac
     9121 ctacctgttg agaacaatcc agccttggcg cgtgaactga tggacttctt tgacgaaaaa
     9181 gacggaatcg agttgattga tatcgaacct gctatgacag gcgaggactt tggttatctc
     9241 ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg atagtcccta cgctcttcat
     9301 caccctcaaa tgagtcctaa ggaagaagtc ttagctattg gggtggctgc ggtgtctagt
     9361 ttcctgaaaa agaaggcagc agaatagagg gcttgtatat gaaatcggaa ttacgcaagc
     9421 aagtcttgca tgaaatgaag gctttatctc aagaacaaaa acaggctata gaccaagctt
     9481 taaccgagcg aattttacaa cacccctttt atcaagaagc caaggtcatc gcaacctacc
     9541 tctctttttc tcatgagttt caaacgcggg aactgattga gcaggcgctg aaggacggca
     9601 agaaggtttt aatacccaaa acttatccca aggggcgcat ggagtttgtg gtctatgatc
     9661 cgcaacagtt ggtaaaaact tcctttgcct tactggagcc acagggagat ttggaagtgg
     9721 tggatgtatc tcagattgat ttgattcatg ttcctggcct ggcttttacg acggatggat
     9781 atcggattgg atatggtgga ggttattatg accgctatct ggaacatttt tctggtcgta
     9841 ctttgagtac ggtttatcct tgtcaaattc aggactttat acctgaaaac catgatattc
     9901 ctgttcagga ggtattaatt gatgaaggaa atctttgata gacgttaccc tgtgacgagt
     9961 ttcttcctct tagtgacggc cttggtattt ttactaatgt tggtcactgc aggcggaaac
    10021 tttgacaggg cagatacatt atttcgattt ggagccatgt atgggccagc tattcgcctc
    10081 tttcccgagc agatttggcg tctcttgtct gccatttttg ttcatattgg gtgggaacat
    10141 ttcattgtta atatgctttc actttattat cttggaaggc aggtagagga gattttcggt
    10201 tctaagcagt ttttctttct ctatctttta tcaggaatga tgggcaatct ctttgttttt
    10261 gtatttagtc ctaaatcctt agcagcaggc gcctctacct ctctttatgg gctatttgcc
    10321 gcgattatca ttcttcgcta tgcaacgcgt aatccttata ttcagcagtt gggccaatcc
    10381 tatctgacac tttttgtggt taacattatt ggaagtgttc tgattccagg aatcagccta
    10441 gcaggccata tcggtggtgc agttggtggc gcatttctag cagttatctt tccagttaga
    10501 ggagaaaaac ggatgtataa caccagccag agattaggag cggtagtctt gttcgtagga
    10561 ctcgccattt tgcttttcta caagggaatg ggaatgtgac gaagttgaaa gaaaatttgt
    10621 gtaatgaaaa aagataaggc attttttgaa ccttatcttt ttattttatt ccttctcagt
    10681 caattctttt ccaagttgga tgaggtaatt cttcaaatca tctttgactt gtgggtgttt
    10741 gagggcgtag tcgatggatg ttttcatgaa gccaaacttg tctccgacat cgtaacgagc
    10801 ccctttgaac tcacgagcaa atacacgttg tgttttattg agggtgtcga ttgcatctgt
    10861 cagctgaatt tcatttcctg cacctggagc ttgcttttcg agaatctcaa aaatttcagg
    10921 cgtgaggagg tagcgtccga taatagcaag gtcgctagga gcgtcctctg gagctggttt
    10981 ttcaacaaag gtttcaacac tgtaaagacc atcttttcct tcgccttgcg gagcaataac
    11041 cccgtaagca gatacttcgt catgagggac tggcatgaca gcgatagtag acgcgtgggt
    11101 acgctcgtag tcatccatga gttgtttggt aagtggaaca gccttttcgt ctgtgatatc
    11161 catcaagtca tcaccaagca taacgacaaa aggttcattt ccgacgaaag ccttggcttg
    11221 caaaacagca tctccgagac cgcgtggatg agtttggcgg ataaaatgca gacgcatgcc
    11281 agttgtttca tcaactagct tcaaaagatc tgttttccct ttttctttga ggttatattc
    11341 caattcgaag tttgaatcga agtggtcctc aatagaacgt tttgatttac cagtgacaac
    11401 tagaatatct tcaatacctg atttgagagc ttcttccacg ataaactgga tagttggttt
    11461 gtctacgatt ggcaacattt ctttggcaag ggccttggtt gctggtaaaa atcgagttcc
    11521 tagtccagca gcagggatga ctgcctttct aacttttgat gtcataaaaa tcctttctat
    11581 agagggttaa gaccactcat tttctgcttt aaattcattg ttcatgatgt cataaatggc
    11641 atctttgata ttggttccgt gataaataac ttggtaaata gcctgtgtaa tgggcatata
    11701 gactccaagt tcttgggcta gttcataggc tgctcgagtc gttgaaattc cttcgattac
    11761 catgcccata ttagcttcta tatcagctag ggattctcct cgtccgagag catctccagc
    11821 tctccagtta cgagagtgga tggaagttcc tgttacgatt aaatctccca caccagataa
    11881 gccgctatag gtcaatggac tggccccgag tgctacccct aggcgggtga tttctgctaa
    11941 acctcgagcg atgatggctg ccttagcatt atcaccaaat ccaagaccat gtaaagctcc
    12001 agcaccgaca gcaataatat ttttaagagc accagcagtt tcaaccccga taacatccgt
    12061 attggtataa agtcggaagt agtgattact aaatagctcc tgaacgtatt gagctgtttg
    12121 taaatcttta gaagcagcag ttattaaagt taggtcacgc acaatggtct cttctgcatg
    12181 actaggccct gaaacaacga cgatatcact acggagatgt tcaggaattt cttcttcaag
    12241 aatggttgat aatcgtttat ggctatcagg ttctaatccc tttgatgcgt gcatgatgat
    12301 aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca agtcgtgtca cttttgttgg
    12361 gacaacaaac aaaatcgcat ccacattttt caatgtttct gctaagtcgg tgtaggcaat
    12421 gatattttcg tctagaacga catctttaaa gtagtgctta ttagtatggt gtgtattaat
    12481 ttcattgatt tgctcgggaa gatttcccca aatacgtacc tcgtgtccat tgtcatttaa
    12541 gacttgtgaa agggcggttc cccaagaacc aggccccaag acggcgacgg tttgtttttc
    12601 catgatttcc tccttgatag agttctttct cttattctat catattctag gggattttgc
    12661 acttgcatta ctggggtatg gtggaaaaat acacaaaaac cgagatgaag ggataaactc
    12721 ttagtcttta gttaaagtga gaagaaatct gatagatagt ttgattcagt atatgctata
    12781 aatagaggta aacaactcag gaggttcttg taggttgcga gagtttggcg aaaaaattaa
    12841 aagattacgt ttggctaaaa aaatcagtcg ttcagaattt tgtggtgatg agtctgaatt
    12901 aagtatccgt caattaatta gaattgaaaa tggagaatcc agaccaacac taacaaagtt
    12961 aaaatatatc gctgaacgtt tgggggttga agattacaag ttgatgccaa gttatataga
    13021 gttggataag gaatacctag aattgaagta tttcttgatg aggactccta catacgaaga
    13081 tgaaactatc gcccaaaaga aagagagcgt tttgataaga tttttgaaga gtattatgat
    13141 aggctacctg aggaagaaag atttatcatc ccaaattatt catatctggc actaacgaac
    13201 tacacagttc aaaaattacc agaaaagcta gttgaaatac tgtccttctg gtgattttgc
    13261 ttaatttctg atgttcgaga catgattgag gagatttgtc ttgatttttt cctaaaaaag
    13321 ggcagactcc tcccttggtt tgatgctcga tttttaaaat aaggtgactt attctgttag
    13381 gcagttttcc cctgccctct agctctatat ggaacaggga gtatcagatt tatctggcag
    13441 attgtatcaa ataaggcttg aaactgtttt gaataggcgt atagacttga gaatttgaga
    13501 atatgtcgtc tagcagtaga gacatatttt ccggcaatat gaaggaagag acgtcgaaaa
    13561 cgcttgatag tcaaggcctt tacttcatca ccagctagct gctttaaaaa gaggtagaga
    13621 ttataagcca gacagcccat catcatacgt acttcattct taatcatggt cgaactatct
    13681 gtcttatccc caaagaatcc tgctttcctt tctttgataa agttttcagc attccctcgt
    13741 tcacgataga attgaaacag ctcctcacta tctacactgc ttaaattgtt caaatgaact
    13801 tttttacaac cataaaaaag agcacttaat cgtactcttc tcttttaagg attagttgaa
    13861 ttaattcaaa tccactagtt ctcctgtttc taggtagact aggcgttcac aaatgttagc
    13921 aatgtaatca gcgaagcgtt ccagatgccc tatgatataa aggtattgag ttccattggg
    13981 aattgaggtt tcttggtctt tcataagtcc aatgatttcc tttgataagg cataataata
    14041 ttggtcaatc tgttcatctt tttgagcaat actaatagct tttgaggctt ggtgcaaagg
    14101 aaaggcaacc aataaatcgg ctagcatgct gagggataat ttacccattt ggtgtaactg
    14161 ttcttcgtca ggggctagtt gattttcttt tagttgcaaa acagctttgg caatgcctgc
    14221 catatggtct cccatacgtt caaggtctga acaagaagac atgatgctaa tcacaaatcg
    14281 aaggtcagac acttgtggct gctgcaaggc taacaaacgg gcacaggtca attcgatagc
    14341 gctttgacct tggttgatag catgatcctt attgataatt agctctgcca tctccttgtc
    14401 tttggaggct aaggccagta aggcttttga agctgtttca aggacaagtt gccctagtcc
    14461 taaaaaggat tgttctaatt catgcaattc taagtcaaat tgatttctca taggtttttc
    14521 cttccttatc cgaaccgtcc tgaaatatag tcttctgtgc gctgatcttt tggattggta
    14581 aacacgtcaa cggtatctcc aaattcgcaa atttctcctg ttaagaaaaa agcagtttta
    14641 tctgaaatac gtgaagcttg ttgcatgtta tgggtaacaa tgataatcgt ataatccttt
    14701 tttagttgct gaatgaggtc ttcaattttt aaagtggaga tagggtctaa ggctgaagtc
    14761 ggctcatcca ttaacagaat atcaggttct actgctaaag ctcgcgcaat gcaaaggcgt
    14821 tgctgctgac cgccagataa ggacatagca ctctttttaa gatcatcttt gacttcttcc
    14881 caaatggctg ccccttttaa agatttctcc actaaggcat ctaattgttt tttgtctcga
    14941 ataccatgtg tccttgggcc ataagccacg ttatcataga tagacatggc aaagggatta
    15001 ggctgttgaa aaaccatccc tacacgctta cgtagctgat taaggttgaa tttgctacta
    15061 taaatatctt gctcatctaa gaggacttgg ccttcaatat ggcaagaagg aaccaaatcg
    15121 ttcatccgat taagggtttt tagaaaagtt gatttaccac aaccagatgg gcctatcaag
    15181 gcagtaatct gtctttctgg taattgaatc gaaatatttt ttaaggcttg aaaatccccg
    15241 taaaataagt ctaggtgtct gactgaaaat gttcccatac taggaagctc ctttcacaag
    15301 ttttcgagat aataagcttg atagagtatt tatcattaaa acagtaataa tcaaaatcac
    15361 accggtagca taggcttcat tgacatgtag cccctcactt gacagcatat acatatgcag
    15421 ggctagagaa cgacctgaag acatgagact acttggcgta ttggtagagg tacctaatgt
    15481 atacatgagg gcagctgtct caccaacgat acggccaata gctagtatca ctccagctaa
    15541 aatacctggc atggcaactg gtagaacaat tctaaaaaca gtccgtaact tacctgcccc
    15601 aagtccataa cttgcttgac gcatgctatc actaacagat aaaagggctt cttctgttga
    15661 gcgaataatg actggcaaca ccatgatgac tgaggttaag attcctgata acagagagta
    15721 ttgaaaacct aagaagacta caaagaagag catgccaaac agaccaaaaa caatggaagg
    15781 aatcccagac aaggtatctg aggccaatcg catgatttta acacaaaggg aatctttttt
    15841 tgtatattcc acaagataaa aaccagcaaa aatccctatg ggcaaggcta aaagaagagc
    15901 accaaagacc agaataacgg tggaaataat cgctggcata agggaaatgt tctcagaagt
    15961 ataagtccaa gaaaagaggg atagacttag atgaggtaag cctttgatga ggataaaacc
    16021 aatgattaaa aagagagagc caaaggttaa agctgaaaaa caataaacga gaagttttag
    16081 caggtattta ctcataagat gattttcctt tcaagtaggc aaagtaggca ttaatcaaga
    16141 gaataaggaa aaagagaact gctgaggttg caataagggc ttccctatgc tgacctgatg
    16201 cgtaagccat ttccagaaca atattggttg ttaaggttct ggttcctgaa aagagtccac
    16261 ttggaataat cggctggttg cctgccacca aaataactgc catggtttca cctactgcgc
    16321 gaccgattcc taaaataact gctgataaaa taccagatct cgcagctggc aagatgacac
    16381 taaaaatact ccgttcatga ctagctccta gagccaagct accagaataa tacgttttgg
    16441 gaactgttcg gatagcagat tctgacaaac tgataatggt tggcaaaatc attattccta
    16501 atagtaacga agcggttagg acactcatgc catttcctaa aaagcttcta atccaaggca
    16561 ccaataattg taggccgaaa aaaccataaa caatagatgg aatggccgcc atcaagttga
    16621 tagctgattt taagaagcca tagacgggct ttggacaata ataaaccata aacaccgatg
    16681 tcaagatgcc tgttggcacc ccaatcacaa tcgctcctaa ggtaattaat aaggaaccaa
    16741 cgatcattgg taaaatacca tagcttgccg gaatgttcgt tggcgaccaa tcactgccta
    16801 ataaaaaacg ggcaaagccg tagttagcca tgaaaggtaa gccattacta aaaataaaga
    16861 aacagattag caaaatagct acaacagcta ctgttgcact catgaaaaaa attgccctaa
    16921 aaactgcttc tttgaaggct tgttttatca catcttgtcc tttctagtga agaaagtaag
    16981 ggagatacga cacctcccta cttgccttct ttatcttatt gtacgatgaa acgtctgcat
    17041 ctctttagag atttatggag caaacatttt atttaatctt gtcccaggtg gttaatttgc
    17101 cactaaaaac gtctgcaagt tcagccatac tgacttggct tgccttattg tcattattga
    17161 ccacaacagc aataccgtct aaagcaatag tatcatgggt gagactctta ccttcttcag
    17221 gagttaattc cctagaaacc ataccaatat cagcggtttt ctccttaaca gcggtaatac
    17281 ctgctgaaga cccattagag gtaatatcaa tcgtaacttc tggattttct tttttataag
    17341 cttctgctaa tttttccatt aaagaagata ctgaagtgga acctacaaca gacaacttgc
    17401 ctgataagtg ttggctggta tattctgtgg tttcggtttt agcttcaata aatttattat
    17461 ctgtgaccac ttgttgacct tgtttggagt ggataaagct gataaaatct tgacctagct
    17521 tggaaagatt agaagaccaa acaatgttga agggacgttg aagagggtat ttaccatcta
    17581 aaactgtgtc tcgactagcc ttgacaccat caatctctaa agccttgaca gatttcgtta
    17641 aagatcccaa ggagatgtag ccgatagcat tagcattccc ttgaactgct gagagaacac
    17701 cttctgtact attttgaatc acagctgttt tggcagtgtt gtcaattttt ttatcaccgt
    17761 cttttttgag aatccctgtg atttctgtga aggcaccccg tgtcccagag ccattttctc
    17821 gtgaaatcac ctcaatcgtt cctgaagctg actgtttgga agcagctgac tgattgccac
    17881 aggcaacaag cccaaatcct gataagccaa tggctgcaag agtaagcatt tttttgaatt
    17941 tcataataat cacctttatc tctatgtatt tttcttgtgt aggcttacta catttatagt
    18001 ctaacaagtc tttgtaaagg tttatccctg attcatgtaa agattgtgta aagaatcaaa
    18061 aaaagccact tttgaaaaat ggctgctcct aaaaatagct ttaaaaatta ttagtcctgt
    18121 gcgaaagatt ggttaggaag aaaaatcgtg aagcaactgc ctctgccaag ctgactcgtc
    18181 accgtgactt ggccacctaa taattgactg agttctttga caatggcaag gccaagacca
    18241 gtgccaccag tttgtctgct tcgaccttta ttaactcggt aaaaacgttc aaaaatacga
    18301 tcctgctcta attgactaat accaatccct gtatctgata cagaaatctt aatgccttcg
    18361 ttcacctttt gggtcttgac ctcaattttt cccccttgtt cagtgtaacg gatggcattg
    18421 gataaaagat tgagtaagat ttgggaaagt aattgactat ctgatacgag ggtgacatca
    18481 tctggcacct gcacctttag ctgtaaatcc ttcttcttga gctgaggttg caagctttga
    18541 gtcaaatcct gtacaaattc tgccaaagaa agggtcgtcc attgtatagg catttgttga
    18601 gccttagata aggtaagaag atgctcaaca atatgctcaa gacgcaagct ttctttgtaa
    18661 ataatgtcta gaaagtcatc cttgagcgct tcttcttcag ctgacatccc cttaatggtt
    18721 tcagcaaagc ccttaatcga agtaactggt gtcctcaatt catgggaggc atttgagaca
    18781 aaggctaaat ttaacttttc ataagttcta atcgttgtta aatcatatag caagacgagc
    18841 acagcttcca cagattgggt gggactaaaa acgggaactg ctgtcacttc taaaatcaag
    18901 tcaccctcat gaaacccact tacttcttgt tttaaccttg ttttttgatc aaaggcttgg
    18961 tgaactaaat tccgaatatc catccgtttg aggtcatcaa gtgaacttat gtcgccgtcc
    19021 acatcgggaa aataatgagg cagagagcga ctggataata acatctgacc ttgagcggaa
    19081 actaaaaacg tccccatggt taggtgcgac agaagaacct ccattgtttc ggctaggtcc
    19141 ttgtattgct gatcctgttg ggagactttg gtttttaggc cagacacata ctgagccaaa
    19201 gactttaagt cttcttgccc tttttctaaa aagtattcac tactggtcaa gagaggttgg
    19261 tgcaaggtct caaaagcaac ttcccatttc caaaggcaaa agagccagta gccaccttgt
    19321 cccaaagaaa gggctagaag aaagagaccg atgcctttac tgatccaagt taatgccatc
    19381 cctgcaatca gaatgaggct aacacttaga ttgactagcc aaaattgaag gtagcgtttc
    19441 atctataact ccttgaactt ataaccataa ccccgaatgg ttcgaataaa ttgaggggct
    19501 ttaggattgt cttcaatttt ttcccttaac ttaccaatat gaacgtccac caaacgtgtt
    19561 tcctgcccaa agtcataccc ccagatacgt tccaaaagac gctctctagt cagtgtcatg
    19621 ttgggatgtt tcataagata gagcaagagt tcaaattctt ttggggtcaa actcagtaac
    19681 ttattcgcct tgtagacttc atgacgctca gggtatactt tcaaggtccc aaatagccaa
    19741 gaatcgtcag cgatattatc tgaatcatct ccttcttgtt ctcctttagt tcgcctgagg
    19801 acagccttga cacgcgccag caattctcta gggctaaaag gcttggtcag gtagtcatca
    19861 gcccctaatt ccaaggccaa aaccttatca aattcatcac ttttcgcaga aaccatcata
    19921 attggagttt tgacgccttt ggctctcagc cgcttacaaa cttccatgcc atctaattgt
    19981 ggtaacatga tatcaagcaa gataaaatca aagggttctg tttctgccaa agctaaggcc
    20041 ttccgtccat ttgttaccaa ttgagtagaa aagccttcct tacttaaatg gtagtcaagc
    20101 aatttcagaa tgtgttcttc atcatccact aataagactt gttttgtcat ctattatctc
    20161 ctattggtaa cattataaca caattatcag aaatcctaac attgctaaat cagattaaat
    20221 ttgcttatca agactagtat ctggtcaaac gctcaatcat ctccttgtgc tctggatagg
    20281 tcgccagtag atctaccctt tcaaataatt caaaatcctc aaattcaaaa ccaggagcaa
    20341 caagacaaga aaccagagca tcatccttat caactgttga tccccaaata gtgcccttag
    20401 gaacacagta gtgaagttgt tgccctttgg atatgtccag gcctaaagtg actgcttcgt
    20461 agtgaccatc tgctgtaatc atgtgaacag taagtgggga tcctgcatga aaataccaga
    20521 tttcatctgc tgtcaatcgg tgaaaatgtg aaggattcgt ttcttctaat aagaaataaa
    20581 tactggtata aagcgccctt cccttaccag caaggtttat agtgtctgaa gctttttttg
    20641 tttgtctaaa atagccacct tcaacatggg gagctaactc tagagttctt atcaagtctt
    20701 ctttatccgt cggagccaat gggttgaagt aactcttgtt caaagtggtt ttacgatttc
    20761 aagaactcct ctcagttctg aggacacggt aatgattgat gcgacggaag taaaaatcaa
    20821 tcgccctaaa agaagaatta gcgaat
//
LOCUS       DAVMEF010000018        20725 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMEF010000018 DAVMEF010000000
VERSION     DAVMEF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.018"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            23..317
                     /locus_tag="VYQ30_000481"
                     /pseudo
     CDS             23..317
                     /locus_tag="VYQ30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..1687
                     /locus_tag="VYQ30_000482"
     CDS             473..1687
                     /locus_tag="VYQ30_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1965644.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            1832..1966
                     /gene="rpmH"
                     /locus_tag="VYQ30_000483"
     CDS             1832..1966
                     /gene="rpmH"
                     /locus_tag="VYQ30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1965645.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            2250..3143
                     /locus_tag="VYQ30_000484"
     CDS             2250..3143
                     /locus_tag="VYQ30_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1965646.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            3448..4221
                     /locus_tag="VYQ30_000485"
     CDS             3448..4221
                     /locus_tag="VYQ30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1965647.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            4221..4781
                     /gene="rnmV"
                     /locus_tag="VYQ30_000486"
     CDS             4221..4781
                     /gene="rnmV"
                     /locus_tag="VYQ30_000486"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1965648.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            4853..5752
                     /locus_tag="VYQ30_000487"
     CDS             4853..5752
                     /locus_tag="VYQ30_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1965649.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQEQTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            6021..8051
                     /locus_tag="VYQ30_000488"
     CDS             6021..8051
                     /locus_tag="VYQ30_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1965650.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMVVLKGK"
     gene            8053..8694
                     /locus_tag="VYQ30_000489"
     CDS             8053..8694
                     /locus_tag="VYQ30_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965651.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            8687..8845
                     /locus_tag="VYQ30_000490"
     CDS             8687..8845
                     /locus_tag="VYQ30_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965652.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFSSIEG"
     gene            9231..10103
                     /gene="rsmA"
                     /locus_tag="VYQ30_000491"
     CDS             9231..10103
                     /gene="rsmA"
                     /locus_tag="VYQ30_000491"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1965653.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            10105..10983
                     /gene="rsgA"
                     /locus_tag="VYQ30_000492"
     CDS             10105..10983
                     /gene="rsgA"
                     /locus_tag="VYQ30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1965654.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            10994..11650
                     /locus_tag="VYQ30_000493"
     CDS             10994..11650
                     /locus_tag="VYQ30_000493"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1965655.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRSHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            11613..12275
                     /locus_tag="VYQ30_000494"
     CDS             11613..12275
                     /locus_tag="VYQ30_000494"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1965656.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTAEERQVIQKRAQHFVQARPEKDDTDLELALLTIFKQNPQTQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            12277..13533
                     /locus_tag="VYQ30_000495"
     CDS             12277..13533
                     /locus_tag="VYQ30_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1965657.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            13523..14464
                     /locus_tag="VYQ30_000496"
     CDS             13523..14464
                     /locus_tag="VYQ30_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1965658.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            14731..15558
                     /gene="purR"
                     /locus_tag="VYQ30_000497"
     CDS             14731..15558
                     /gene="purR"
                     /locus_tag="VYQ30_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1965659.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            15625..16875
                     /locus_tag="VYQ30_000498"
     CDS             15625..16875
                     /locus_tag="VYQ30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1965660.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            17001..17795
                     /gene="pflA"
                     /locus_tag="VYQ30_000499"
     CDS             17001..17795
                     /gene="pflA"
                     /locus_tag="VYQ30_000499"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1965661.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(17870..18796)
                     /gene="yidC"
                     /locus_tag="VYQ30_000500"
     CDS             complement(17870..18796)
                     /gene="yidC"
                     /locus_tag="VYQ30_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1965662.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18874..19152)
                     /locus_tag="VYQ30_000501"
     CDS             complement(18874..19152)
                     /locus_tag="VYQ30_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1965663.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            19186..19926
                     /locus_tag="VYQ30_000502"
     CDS             19186..19926
                     /locus_tag="VYQ30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1965664.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            19982..20665
                     /locus_tag="VYQ30_000503"
     CDS             19982..20665
                     /locus_tag="VYQ30_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1965665.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
BASE COUNT         6035 a         3930 c         4832 g         5928 t
ORIGIN      
        1 gaaagaagtg aaggaaggga gaatgatgaa aaactctttt caaaaatcaa atttccttta
       61 ttatggaatt atcctagttt cagtcttggt agaagtgatc atgatctgtc aattagagag
      121 tctggtaccg cttctttatc caagttttat tggattttta gtttttcatg tactctacca
      181 tctaatctta tttctagttg caaaacgaag tgggcgttgg gattacttga tgatatgggg
      241 gcttttcctc atgtttaatc ttctttatga ctcctttcta ggcttgcttt tttctaggtt
      301 tatcttttgg tatgtgatgt gtctttgcaa aatacctgct atctctacgt tcttaacctc
      361 gcctttagca aggtttttct tctatacgaa actagtaaca catttcagta aacttgtcgc
      421 attctctttc tagtggtata atgttactat cctgatgaat tgaggaaaca agatgaaaga
      481 atataacaag tctagtaagt tagagcatgt tgcttatgat atccgtggtc ctgtattgga
      541 agaagccatg cggatgcgag cgaatggaga aaagatttta cgtctgaata caggaaatcc
      601 agcagaattt ggctttacag cgccagacga ggtcattcat gacttgatta tgaatgcgcg
      661 tgatagtgaa ggctattctg actccaaagg gattttctca gcccgtaagg ccatcatgca
      721 gtattgccag ctgaagaaat tcccaaacgt agatattgat gatatttacc ttggaaatgg
      781 tgtcagtgag ttgattgtca tgtccatgca ggggcttttg gacaatgggg atgaggtctt
      841 agtgccaatg ccagactatc ctctatggac ggcggctgtc agcctagctg gaggaaatgc
      901 cgttcactat atctgtgatg aagctgtgga atggtaccca gatattgacg atatcaagtc
      961 aaaaatcact tccaatacca aggcaattgt ccttatcaat ccaaataacc caactggagc
     1021 cctttatcct aaggaactct tgctggagat tattgagatt gcccgtcaaa acgatttgat
     1081 tatctttgcg gatgaaatct atgaccgcat ggtaatggac ggacatgtgc atacgcctgt
     1141 ggcgagcttg gcaccagatg tcttctgtgt cagcatgaat ggtctgtcaa aatcccaccg
     1201 catagcaggt ttccgtgtgg gttggatggt cttgtctggc cctaagactc atgttaaggg
     1261 ctatatcgaa gggctcaata tgctgtccaa tatgcgcctt tgctctaacg ttttggccca
     1321 acaagtcgta caaacttcct tggggggtca ccaatcagtc gatgaattgc ttcttcctgg
     1381 tggacgaatc tacgagcaaa gaaatttcat ctataatgcc attcaagata ttccaggttt
     1441 gtctgccgtt aaacccaagg cggggctcta tatcttccca aaaatcgacc gcaatatgta
     1501 ccgtatcgat gatgatgagc agtttgtcct tgatttcttg aagcaggaaa aggttctctt
     1561 ggttcatggt cgaggcttta actggcagga accagaccac ttccgtatcg tttaccttcc
     1621 tcgtgttgat gagttagccc aaatccaaga aaagatgact cgtttcttga aacagtatcg
     1681 tagatagggc ttgcattcga aaaagctgga aacatttgcc tagagctatt gacaaaagtg
     1741 aaagaatcag atagaatagt aaagtatgtt tagtaactga tcactttata gccggctaaa
     1801 cgaatacaaa atctatgagg aggtattcat cgtgaaacgt acttatcaac caagtaaact
     1861 tcgtcgtgcg cgcaaacacg gattccgtaa ccgtatgtca actaaaaatg gtcgtcgcgt
     1921 attggcagct cgtcgtcgta aaggacgcaa agttttggct gcataatcca aacgaattct
     1981 atcaaaaatc agtaggaact cgagtctact gatttttatt tttgtaaaaa agttcagtag
     2041 atgcaaatgg attcggaagc gatgttacag tagattgaaa ctagaatagt acacctctgt
     2101 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct gttattattt
     2161 tattttacta taaagttgaa gtaggtggag atggtacagc aacaatcgtc tttaaagatg
     2221 gttcagctat tacaattcca ggaaatcaat tggtagcaca agatccaaaa gcacaagata
     2281 gcactaaact gactgctgaa aaatcaactg ttaaagcacc tgctcaaaga gtagatgtaa
     2341 aagatataac tcatttaaca gatgaagaaa aagttaaggt tgctatttta caagcaaatg
     2401 gttcagcatt agacggagcg acaatcaatg tagctggaga tggtacagca acaatcacat
     2461 tcccagatgg ttcagtagtg acgattctag gaaaagatac agttcaacaa tctgcgaaag
     2521 gtgaatctgt aactcaagaa gctacaccag agtataagct agaaaataca ccaggtggag
     2581 ataagggagg caatactgga agctcagatg ctaatgcgaa tgaaggcggt ggtagccagg
     2641 cgggtggatc agctcacaca ggttcacaaa actcagctca atcacaagct tctaagcaat
     2701 tagctactga aaaagaatca gctaaaaatg ccattgaaaa agcagccaag gacaagcagg
     2761 atgaaatcaa aggcgcaccg ctttctgata aagaaaaagc agaactttta gcaagagtgg
     2821 aagcagaaaa acaagcagct ctcaaagaga ttgaaaatgc gaaaactatg gaagatgtga
     2881 aggaagcaga aacgattgga gtgcaagcca ttgccatggt tacagttcct aagagaccag
     2941 tggctcctaa tgctgctcct aagacaacaa gtgcaccgca agcaactgca ggaacaatgc
     3001 aagatgttac ctaccagtca cctgctggca aacaattacc taacacaggt tcagcatcaa
     3061 gtgcagcact tgctagtctt ggtctagtgg tggcaacaag tggttttgct ttgctaggaa
     3121 gaaagactag acgtagaaaa tagaacagct agaaaattct attctctact taaagttaga
     3181 ttataagggg gattttgaga agtcatcaat cctagtgatg ggtgagaaaa gtgagaaccc
     3241 aagataatca catactttag ctgaatagga atattctatc aatgtagcca atctcttctg
     3301 tctctaactg tggaatagga gatgggcaat atcggataga aaagatagca gaatagctct
     3361 ctattgaaga gaggagggga aaccgaaaaa ttaggtgccc ctcctctttt ttggtataat
     3421 agaagataga aaacgaggtt agaagagatg atttttgata cacatacaca cttgaatgta
     3481 gaagaatttg caggtcgtga ggcagaagaa attgccttgg ctgctgagat gggtgtgaca
     3541 cagatgaata ttgttggttt tgataaaccg acgattgagc atgccttgga gttggtagat
     3601 gagtatgagc agctctatgc gactattggt tggcatccta cagaagctgg tacttataca
     3661 gaggaagttg aggcttactt gttggataag ttaaaacatt ccaaggttgt ggctttaggt
     3721 gaaattggct tagattacca ttggatgaca gcgcccaaag aggtgcagga gcaggttttt
     3781 cgccgtcaga ttcagctatc taaggacttg gatttgcctt ttgttgtcca tacccgtgat
     3841 gcgctggaag atacctatga gattatcaag agtgagggcg ttggtcctcg tggtggtatc
     3901 atgcattcat tttcagggac gcttgagtgg gcagagaagt ttgtggatct tggtatgacc
     3961 atttccttct caggagtggt gacttttaag aaggcaactg acctccaaga agcagctaaa
     4021 gagttacctt tggacaagat gttggtggaa acagatgcgc cttacttagc acctgtaccc
     4081 aagcgtggtc gtgaaaataa aacagcctat actcgctatg tggtcgactt tatcgctgac
     4141 ttgcgtggta tgacgacaga agagctggcg gtagcaacga ctgcaaatgc agaacgaatt
     4201 tttggactgg acagcaagta atgaaagaga aaatttctca agttatcgtg gttgaaggtc
     4261 gcgatgatac ggtcaatctc aaacgttatt tcgatgtgga gacctatgag actcgaggtt
     4321 ctgccatcaa tgctcaggat atagagcgga ttcagcgcct gcaccaacgt catggagtca
     4381 ttgtctttac agacccagat tttaatgggg aacggattcg gcgcatgatc atgatggtca
     4441 ttccaacagt tcagcatgcc tttctcaagc gagatgaagc tgttcccaag tccaagacca
     4501 aggggcgttc tctgggaatt gagcatgcca gctatgaaga cctgaaaacg gctctagctc
     4561 aggtgacaga acaatttgaa catgagagtc agtttgacat tagtcgtagc gatttgattc
     4621 gccttggttt tctagcaggg gcagacagcc gtaagcgtag agaatatctc ggagagactc
     4681 tccgaatcgg ctattccaac ggcaagcaac tcctcaaacg cctagagttg tttggggtta
     4741 ctttggcaga agtggaagaa gctatgaaat cttatgagta ggaaagatgt agccgttaca
     4801 attttttaag tttcacagta tttttcgaag caggtagaag aggaggcgtc tgatgttaat
     4861 tggtcaaaaa attaaagaga ttcggataga aaaaggaatt agtcgtccag atttttgtgg
     4921 agatgagcaa gaacagacag ttcgtcaact gtcgcgaatt gaaagtggag cttcgcaacc
     4981 gagtttgccc aagttagact atattgctcg ccggctagga gttccagttt atagccttat
     5041 gccggatttt tcagctcttc cttctgctta tttagaattg aaataccaga ttttacgtga
     5101 accaatctat ggtaaagaag aggagtacga taagaaggaa gcgtgtttgg aagagattta
     5161 taaaacatac tttgataatc ttcctaaaga agaacaatta gcatgtgaag tattgcaggc
     5221 gtgtttggat acttctagaa ctagaaggcc tgaatatgca gagttaatac ttgaggaaca
     5281 tatgcctcag attatagaaa aagaagctta ttcaataaat gatatgttgt tgattcgttt
     5341 gtttttttat caaatgctca ttagaaaaga tcttgccaaa tttataaatc aaatcgaaaa
     5401 gctaatgctc tttcttttgg aacagaagaa ggtaactcaa atagagaatt actttataat
     5461 tagagatact cttatttcag gaatgtgttg tcttgaaaag gtaggagtaa ctgattgttt
     5521 taatgattat ctatcgtgtt tacaagaaat tatggataaa actcaagatt atcaaaagaa
     5581 acctcttgta tttatgtttt tgtggaagca agcattaaga gaagaaagag attttagttt
     5641 agctgaatca ttttatcagt cttctaaaac atttgcgcag ctaattggag atgaatttct
     5701 agtaaagaaa ttgacagagg aatggcaaga ggatgttaaa aaatatttat aaacatagtg
     5761 aatcagtgac aaaaatgtcc ttgtcctcgt atcgaaacag ttctaaagtt cgtctttagg
     5821 gatgtttttt tagatataag ctaaaaatga cacgaaatgg ttagatttta aggacattga
     5881 tgtccttgat agttcttgaa aaaacggata taatgtattt gaagatgttg ctagcagaaa
     5941 gaacttaata agcttcttaa catcgcagtg actgtggtca ccttctcacg aagtgaccaa
     6001 gtaacttttt ggaggaaatg atgaaacgtt tatttatttt gatttcaatg gtattagtat
     6061 cgctttatat ggtgataact tccgttgacc atcgagaaga gattttattt ggtaactatc
     6121 cttctgttga tgtgacagga atgatgataa atcaaccagt agctagtcgc gaagaggtga
     6181 cagaggcttt gagtcacttg gcggtagagc acaatagtct cattgctcgt cgaatcgttg
     6241 agccaaatga agctggagaa acacgcttta cctatgccac ttatggtgag ggaaagcttc
     6301 cagaaggtct gaccatttcc tccaaggaga gtgcagaaac gagtgattta ttagggtctt
     6361 acttgattgt gtcaggaagt ttggatggag tgagcttaca gaccaccttg aaagagcttg
     6421 gttatcaagg ctttgtttcg aatggagaag atccattttc gatagtctta ctattgacgg
     6481 ccacccctat ggtgctactg agtttagcta tttttctgct gacctttatg agtctgaccc
     6541 tgatttatcg gatcaaatcc cttcgtcagg cagggattcg cttaatagct ggtgagagct
     6601 tgtttggagt tgctctcaga ccagtgttag aagatgtgag acagcttatc tgctcagtgc
     6661 tggtatccag tcttttggga ttggggattc tctggtatca aggtgccttg tttatggcaa
     6721 cggtgcaact ggtcatcatt gctcttctac tttatggatt gaccctggca gggatttcta
     6781 ccttactaag tgtcgtctat ctacttggtt tacaggaaaa tagtctggtg gatctattga
     6841 aagggaaact ccctctcaaa cgtatgatga cattgatgat ggtggggcaa ctcttagctg
     6901 tattggtggt cggatcgagt gcgacagctc tcctacccca ctaccgtgaa atgcaggaaa
     6961 tggagagagc tagcaataaa tggagccagt cctcagaccg ttaccgtcta tcctttggtt
     7021 ggtctagtgc atttgccgat gaagaaggaa cgcgtaagga taatcgtgag tggcagacat
     7081 ttactgaaga acggttagcc aatacagact ctttttatat tatgagcaat gttgacaatt
     7141 tctcagatgg agcagaagtg gacctagatg gcaatcgtct cagtgactac acaccgtcag
     7201 ggaatgttat ctatgtctca ccacgctatc tgatagaaga aaagattacc gtttcttcag
     7261 agtttatgga caagatgcaa aacttgtctg agggagagtt tgggctgatc ttgcctgaga
     7321 gcttgcgaga gcagtctgtc tactaccaag gattgtttac agattacctg caaaactttt
     7381 catctgaaag tgtagaagtg acgagtcaga aacactacct cccacaggta aggctagctt
     7441 ttacagaaat aggacaggaa cgtttcctct ataatgatgg gtacaagaca acacgccagt
     7501 acctaaaaga tccgattatt gtagttctaa cgccgcaagc gactggaaca agacctgttg
     7561 cagggatgtt gtggggaact acggctaata gtgccttgaa actagatcga tatggagaca
     7621 gcatcacagc tctaaaagag caaggtctgt atcacaaggt ttcttacttg gtaaaaagcc
     7681 agctattttt tgccaaggta ctaaatgaca aacgggtgga gttttactct ctccttattg
     7741 ggacgatttt gaccctgtct acggctatct tgttatttga ttccatgaat cttctctatt
     7801 ttgagcagtt cagacgggag cttatgatta aacgtcttgc tggtatgaca atctatgagc
     7861 ttcatggcaa gtatttactg gcgcaaggag gagttctctt gcttggccta gtcctatcta
     7921 gtattttgac aagagatggt ttgattagcg ctctagttgt agctttgttt acgcttaacg
     7981 ccctcttgat tttagtaagg caggacaaaa aagaagaagc tggtagcatg gtagtattga
     8041 aaggaaaata agatgattga tattcaagga ttggaaaaga aatttaatga ccgcgcgatt
     8101 ttctctggtt tgaatctcaa gctggagaag ggcaaggttt atgccttaat cggaaagagt
     8161 ggaagcggaa agacgacgct gctgaatatc ttgggaaagc tagaaaagat agatggagga
     8221 agggttctct atcaggggaa agatttaaaa accattccca ctcgtgagta ttttcgagat
     8281 cagatgggct atctctttca aaattttggc ctcttagaaa accaatcaat caaagaaaat
     8341 ttggatttgg gttttgttgg tcagaaaatc tcaaaagtag aacgtttgga aaggcaagtg
     8401 ggggctttag aaaaagttaa tctagggtat ttggatttag aacaaaaaat ctatacttta
     8461 tctgggggag aggcccaacg agttgccctt gctaagacta ttttgaaaaa tccacccttg
     8521 attttggcag atgaaccaac agcagctctt gatcctgaaa attcagagga ggttatgaat
     8581 ctcttggtgg atttgaaaga tgaaaatcga attatcatca ttgcgaccca taatccccta
     8641 gtctggaata aggctgatga aatcattgat atgaggaaac ttgctcatgt gtgaaaaaat
     8701 ccgtattcgc agggtatctg attatcctag tgccagaggt ggtttagaag atatcctcat
     8761 catggaaaat atgaccaatc atctcctttt ggttcaaatc cgagtgcatg gctatttgct
     8821 tgatttttct agtattgaag ggtaaaggca aaagcattat cgtttgaaaa atttacctca
     8881 aacggttgaa ctgacagtgg atgatgtgga ggaggatgtg gatttgaccc tacccgaaaa
     8941 tcgaagttat caagaagctg atttttttga tactcaatga aaatcaaaga gcaaactagg
     9001 aagctagccg caggctgtac ttgagtacgg caaggtgaag ctgacgtggt ttgaagagat
     9061 tttcgaagag tatgaacgta tgtttcgaga gaagcgctaa gtccctcttt aaagatttcc
     9121 aagactatct ttcttcatga ggaaagatag ttttttggta tgattttcat tcccaaaata
     9181 caaggggaat gtgttacaat agtagtaaca gataatagaa aagagaatag atgagaattg
     9241 cagattatag cgtgaccaag gcagtgttgg agcgtcacgg ttttaccttt aaaaagtcct
     9301 ttgggcaaaa tttcttgacg gataccaata tccttcaaaa aattgtggat acggctgaaa
     9361 ttgatgatca ggtcaatgtc atcgaaatcg ggccaggtat tggtgccttg acagaatttt
     9421 tggctgagcg tgcagcccaa gtcatggctt ttgagattga ccaccgtttg gtgccaattt
     9481 tggcagatac tctgcgtgat tttgataatg tgaccgtagt taacgaagat attctcaagg
     9541 ttgatttggc gcaacatatc caaaatttta aaaatcctaa cctgccaatc aaggtagtgg
     9601 ctaatttgcc ttactacatc acgacgccta ttctcatgca cttgattgag agtggcattc
     9661 ctttttgtga gtttgtggtc atgatgcaga aagaagtagc ggaccgcatt tcagcccagc
     9721 ctaacaccaa ggcttacggt agcttgtcta tcgccgtgca gtattacatg acagccaagg
     9781 ttgcctttat cgtgcctcgt acggtctttg tgccagcgcc aaatgtggat tcagccatct
     9841 tgaaaatggt gcgtcgtcca gagccagccg tagcagtaga agatgagaac tttttcttta
     9901 aggtttccaa ggctagtttt acccatcgcc gcaagacctt gtggaataac ttgacaggtt
     9961 actttggtaa gactgaagag gtcaaggaca agctgaccaa ggctttggac caggcaggct
    10021 tgtcaccaag tgtgcgtggg gaagctctca gcttggcaga atttgccggt ctagcagacg
    10081 cacttaaagg gcaaggactc taagatgcag ggacaaatca ttaaagcctt ggcaggtttc
    10141 tactatgtgg agagtgatgg ccaggtttat caaacacgcg cgcgtgggaa tttccgtaaa
    10201 aaaggccata ccccttatgt tggggactgg gtagatttct ctgccgagga aaattcagaa
    10261 ggctatatcc tcaaaattca cgaacggaaa aacagtctgg ttcgtccgcc tattgtcaat
    10321 atcgatcaag ctgtagtaat catgtccgtc aaggaacctg attttaacag caatttgctg
    10381 gatcgtttct tggttctttt ggagcacaag ggcatccatc ccattgtcta tatttccaaa
    10441 atggatttgt tggaagatag gggagaactg gatttttacc agcagaccta tggtgacatc
    10501 ggctatgact ttgtgaccag taaagaggaa ctcctgtctt tgttaacagg caaggttacg
    10561 gtctttatgg ggcagacagg tgttgggaag tcaactcttc tcaataaaat cgcaccagac
    10621 ctcaatcttg aaacgggaga aatttcagac agtctaggtc gcggtcgcca taccactcga
    10681 gctgttagtt tttacaatct caacgggggt aaaatcgcag atacaccagg attttcatcc
    10741 ttggactatg aagtatcaag ggctgaagac ctcaatcagg ctttcccaga gattgctact
    10801 gttagccgag attgtaagtt ccgtacttgt acccataccc atgagccgtc ttgtgccgtc
    10861 aaaccagctg ttgaagaggg tgttattgca accttccgtt ttgacaatta cctgcaattc
    10921 cttagtgaaa ttgaaaatcg tagagaaacc tataaaaaag tcagcaaaaa aattccaaaa
    10981 taaggagaaa cctatgtctc aatacaagat tgctccgtca attctggcag cagattatgc
    11041 caactttgaa cgtgaaatca aacgtctaga agcaactggg gcagaatatg cccatatcga
    11101 tatcatggac agtcattttg taccgcaaat cagttttggt gcaggtgtgg tcgagagcct
    11161 tcgttctcat agtaagatgg ttttcgattg ccacttgatg gtgtcaaacc ctgagcatca
    11221 tctggaagat tttgcgcgtg caggtgcaga catcatcagt atccatgtag aagcaacacc
    11281 tcatattcat ggcgccctcc aaaaaattcg ttcactcgga gttaagcctt cagtcgttat
    11341 caatcctggc acatcagttg aagccatcaa gcacgtcctt catctagttg accaagtttt
    11401 agtcatgacg gttaatccag gttttggtgg gcaagccttt ctgccagaaa ccatggataa
    11461 ggttcgtgag ttggttgctc ttcgtgagga aaaaggtttg aactttgaaa tcgaagtgga
    11521 tggtgggatt gatgaccaaa ctattgccca agccaaagaa gctggtgcga ccgtttttgt
    11581 agcaggttcc tatgtcttta agggagatgt caatgagcga gtacaaactc tcagaaaaca
    11641 actggactag ggttgccgtt tttgcaggcg gaaaccgcgg tcattatcgg acagattttg
    11701 atgcttttgt tggggtggat cgaggctcgc tctgggtctt ggaagaagac ttgcctcttg
    11761 ctctagcagt tggagatttt gattctgtca ctgcagaaga gcgacaggtg attcaaaaac
    11821 gtgcccagca ttttgtccaa gcccgaccag aaaaagatga tactgatttg gaattggctc
    11881 tcttgaccat ctttaagcaa aatcctcaga ctcaggttac tattttcggt gccttgggtg
    11941 gccgtattga ccatatgttg gccaatgtct ttctgcctag caatcctaag ttggcaccct
    12001 atatgcatca aatagaaatt gaggatgggc aaaacttgat tacttattgt ccagaaggaa
    12061 tcagtcagct agaacctcgt tcagactacg actatctagc ctttatgcca gttcgggata
    12121 gccagctgac tattcttgga gccaagtatg agttgacaga ggaaaatttt ttctttaaaa
    12181 aagtgtacgc ttctaacgaa tatatagata gggaagtgtc ggtaacttgc ccagatggtt
    12241 atgtggtcgt actgcatagc aaggacagga ggtaggatgg aaagtttact tattctatta
    12301 ttaattgcca acctagctgg tctctttctg atttggcaaa ggcaggatag gcaggagaaa
    12361 cacttaagta agagcttgga ggatcaggca gatcatttgt cagaccagtt ggattaccgc
    12421 tttgaccaag ccagacaagc cagccagtta gaccaaaaag atttggaagt ggttgtcagc
    12481 gaccgtttgc aagaagtgcg gattgaattg caccaaggtc tgacccaagt ccgtcaagaa
    12541 atgacagata atctcctcca aactagagac aagacagacc aacgtctcca agccttgcag
    12601 gaatcaaatg agcaacgttt ggaacaaatg cgccagacgg tcgaggaaaa actagaaaag
    12661 accttgcaga cacgcttaca ggcttccttt gagacagttt ctaaacaact ggagtctgtc
    12721 aatcgtggtc ttggagaaat gcagacagtt gcccgtgatg tcggagctct taacaaggtt
    12781 ctctctggaa ccaagacgcg agggattctg ggagaattgc aactggggca aattattgaa
    12841 gacatcatga cacctgccca gtacgaacga gaatacgcaa cggttgaaaa ctctagtgaa
    12901 cgagtggagt atgccatcaa gttacccgga caaggcgacc aagaatacgt ctatctgcca
    12961 attgactcta agtttccact ggcagattat taccgcttgg aagaagccta tgagacaggt
    13021 gacaaggatg agattgaacg ctgtcgtaag tcactcctag caagcgtcaa gcgctttgcc
    13081 aaggatatca agagcaagta catagcaccg cctcggacaa ccaattttgg aatcttgttt
    13141 gttccgacag aaggtcttta ttcagagatt gtgcgcaatc cggtcttctt tgatgatttg
    13201 agacgggaag aacagattat cgttgcagga ccaagtaccc tatcagccct tcttaactcc
    13261 ctatcagttg gtttcaagac ccttaatatc caaaagagtg ccgaccatat cagcaagact
    13321 cttgcaagtg tcaagaccga gtttggcaag tttggtggta ttctggtcaa ggcacaaaaa
    13381 catctccaac atgcctctgg caatattgat gaattattaa accgtcgtac catagctatc
    13441 gagcggacgc tccgtcacat tgagttgtca gaaggtgagc ctgcgcttga tctactccat
    13501 tttcaagaaa atgaggaaga atatgaagat tagtcacatg aaaaaagatg agttgtttga
    13561 aggcttttac ctaatcaaat cagctgacct gaggcaaact cgagctggga aaaactacct
    13621 agcctttacc ttccaagatg atagtggcga gattgatggg aagctctggg atgcccaacc
    13681 tcataacatt gaggccttta ccgcaggtaa ggttgtccac atgaaaggac gccgagaagt
    13741 ttataacaat acccctcaag tcaatcaaat tactctccgc ctgcctcaag ctggtgaacc
    13801 caatgaccca gctgatttca aggtcaagtc accagttgat gtcaaggaaa ttcgtgacta
    13861 catgtcgcaa atgattttca aaattgaaaa tcctgtctgg caacggattg tccgaaatct
    13921 ctacaccaag tatgataagg aattctactc ctatccagct gccaagacca accaccatgc
    13981 ctttgaaacg ggcttggcct atcatacggc gaccatggtg cgtttggcag acgctattag
    14041 cgaagtttat cctcagctca ataagagcct gctctatgcg gggattatgc tgcatgactt
    14101 agctaaggtt atcgagttga cggggccaga ccagacagag tacacagtgc gaggtaatct
    14161 tcttggacat atcgctctca ttgatagcga aattaccaag acagttatgg aactcggcat
    14221 cgatgatacc aaggaagaag tcgttttgct tcgtcatgtc atcctcagtc accacggctt
    14281 gcttgaatat ggaagtccag tccgtccacg tattatggag gcagagatta tccatatgat
    14341 tgacaatctg gatgcaagca tgatgatgat gtcaacagct ctggctttgg tggataaagg
    14401 agagatgacc aataaaatct tcgctatgga taatcgttcc ttctataaac cagatttaga
    14461 ttgataattt aagaaaaatg agcatttttt aggataagaa tgttcgtttt tttatgtgaa
    14521 tatggtataa taagtaaaag acaaaaatga atactcttcg aaaatctctt caaactaggg
    14581 tagtatcgcc ttgtcgtatg tatatatgca ggtatattac agggtttgtc agttctatcg
    14641 acaatctcaa agcagtgttt tgaaccacca gcgaccagct ttctagtttg ctttttgatt
    14701 ttttgaataa aaatggaata ggaaatagaa atgaaattaa gaagaagtga tcggatggtt
    14761 gtcatttcca actatttgat taataatcct tataaactaa ctagtctcaa tacttttgct
    14821 gaaaagtatg agtctgctaa atcatccatc tcagaagata tcgtcattat caaacgcgcc
    14881 tttgaggaaa ttgaaatcgg tcatatccag acagtgactg gggctggcgg aggtgtcatc
    14941 ttcacaccgt ctatttcgag tcaggatgct aaggaaatgg ttgaagactt gcgtaccaag
    15001 ttgtcagaaa gtgaccgtat cttgccaggt ggttatatct atctgtctga tttgcttagc
    15061 acaccagcca tcttgaaaaa tattggtcgt attattgcca aaagctttat ggaccaaaaa
    15121 attgacgcgg ttatgaccgt agcaactaag ggtgtgccac ttgcaaatgc agttgccaat
    15181 gtcctcaatg tctcttttgt cattgtgcgc cgtgacctga aaattaccga aggttcaact
    15241 gttagcgtca actatgtttc aggttcaagt ggtgaccgta tcgagaaaat gttcctttca
    15301 aaacgtagtc ttaaggcagg tagccgtgtc ttgattgtgg atgacttctt gaaaggtggc
    15361 ggaacggtca acggtatgat tagtctcttg cgcgagttcg actcagaact ggcaggtgta
    15421 gcggtctttg cggacaatgc ccaagaagaa cgtgaaaagc agtttgacta caagtcactc
    15481 ttgaaggtaa ctaatattga tgtcaagaac caagccatcg atgttgaggt tggcaatatc
    15541 tttgacgaag ataaataaga gatagaacta aaggttggaa cgattgtccc agcctttctt
    15601 tgcaaacaga atagaaggaa gcttatgaaa acaccattta tcaatagaga agagttagaa
    15661 gcgattgttg ccgagttccc gactcccttt cacttgtatg atgagaaggg gattcgtgag
    15721 aaggcaagag ccgtcaacca agctttttcg tggaacaagg gctttaagga atattttgca
    15781 gttaaggcta ctccaactcc agctattttg aaaattctcc aagaagaagg ttgtggtgtg
    15841 gactgctcta gttatgtaga gcttttgatg agccataaac tggactttct gggttctgag
    15901 attatgttct cttccaacaa cacgccagac aaggaatacg cctatgcacg tgaattgggt
    15961 gcgaccatta acttggatgc ctttgaagat attgaacatc tggagagagt agcaggcatt
    16021 ccagaaatca tctcttgtcg ttataatcct ggaggcgttt ttgaactggg gacagacatt
    16081 atggacaatc ctggggaggc taagtttggc atgaccaagg accagctctt tgaagccttt
    16141 gctatcttga aggaaaaagg agccaagact tttgggattc actccttcct agcgtccaat
    16201 accgtgaccc atctctatta tccagagttg gctcgtcagc tctttgaact ggctgttgaa
    16261 atcaaggaaa agttgggcat ttggctagac tttatcaatc tttctggcgg tattggtgtt
    16321 aattatcgtc cagaccagga gccgaacgat atcgccttga ttggtgaggg agttcgtaag
    16381 gtgtatgaag aagttcttac gtcagcaggt cttggtcagg tcaagatttt caccgaattg
    16441 ggtcgtttta tgctggcacc tcacggtgct ctagtcacaa gagtgactca taagaaggaa
    16501 acctaccgta cctatctagg tgtggatgcc tcagcagtca acctcatgcg tccagctatg
    16561 tacggagctt accatcatat tagcaacgtg acccatccag atggaccagc tgaagcggta
    16621 gatgtggttg gttcactctg tgaaaacaat gataaatttg cagtcaatcg cgaactgcct
    16681 catacagaaa tcggtgattt gctggtaatt catgatacag gtgcccacgg attttcaatg
    16741 ggctaccagt ataatgccaa attacgttct gcggaaatcc tctataccga agaaggtaaa
    16801 gcccgtcaaa tccgccgtgc agagcgccct gaggactatt ttgcaacctt atatggcttc
    16861 gattttgaag aataatctga taatagattg aaaatgaaat tgaaaaacag attgctttct
    16921 aaaaaatagg caaaaatctt gtttttcctt caagtcgtga tataataaaa ctataaaacg
    16981 ttttcaagga aggtaacgat atgtctgaag aaacaattga ttatggacaa gtgacaggaa
    17041 tggtgcattc gacagaaagc tttgggtcag tagatgggcc cggtattcgc tttattgtct
    17101 ttttgcaggg ctgtcacatg cgttgccagt attgccacaa cccagacact tgggctatgg
    17161 agtccaataa gtcacgtgaa cggacggtag atgatgtctt gacagaggcc ttgcgctacc
    17221 gtggtttctg gggaaataag ggtgggatta cagtcagtgg aggagaagct ctcttgcaga
    17281 ttgatttcct gattgccctc ttcaccaagg ctaaggaaca aggaatccac tgtaccttgg
    17341 acacctgtgc tcttcctttc cgtaataaac cacgttacct tgagaagttt gacaaactca
    17401 tggctgtcac tgacttggtt cttttggata tcaaggaaat caacgaagaa cagcacaaga
    17461 ttgtcactag ccaaaccaat aaaaatatct tggcttgtgc ccagtatcta tcagatattg
    17521 gaaaacctgt ctggattcgc cacgtgctag ttccaggatt gacagacaga gatgatgact
    17581 tgattgaact tggtaagttc gtcaagaccc tcaaaaatgt tgataagttt gaaattctac
    17641 cttatcacac catgggtgag ttcaagtggc gtgaacttgg aattccatat tccctcgaag
    17701 gagtcaaacc accaacagca gatcgcgtca agaacgctaa acaactcatg gataccgaaa
    17761 gttatcaaga ttatatgaaa cgtgtacatg gatagaaaag aagcctgatg gaaacatcgg
    17821 gcttttgatt tgcaaaaaga cttagcaaat cagctaagcc tttttcttct tatctcgaac
    17881 gttgttttcc agcgttgcga tttttgtgtt ttttcttgct tgtgatagca gttggttgtt
    17941 caggggtaac gtcttttcgt ccacttggtg ttgagaaagc acttgctttt gatgggttct
    18001 ttgccagttc ttcacggact tttttgcgaa gttttggacg aacgatatag ttgacgataa
    18061 attgctggag aatcatcatg aaaccaccga caacccagta aagtgtgaca ctagctggtg
    18121 agaagaggga gaagacgacg atcatgagtg ggctcatgta aatcattttc ttgatttgtt
    18181 ctctttgcat ttcatcttct actccgtgaa gtgaaaggag cgattgaaga tagtaaagga
    18241 caccagcaca ggcaaccaaa atcatacttg gagaacctag aggaatgcct aggtagcttg
    18301 cttgagcaac cccttcagta tgttgggcag caaagtagat agcagagaag aaaggcattt
    18361 gaaggaggat agggaaacat cctacaccgc caaacatgct gataccgtgc tctttttgag
    18421 cggcaaagag ggcttgttgg gcttcgagtt tttcttcttg agtagtcgct tctttgagac
    18481 gtgtttggtg tggctcaagg acatgcttga gagcgttcat cttttcagag tgaagcgttg
    18541 ccttccatga ttggtagata ccaagtggta agataatcaa gcgtacgata atggttacga
    18601 taatgatagc gacaccaaag cctagacctt tatcagtagc gaagtacttg atggcttcag
    18661 ccataggcgc tccgatcgta ttccaaataa atcctgttgg ctgacctgtg gttttatcga
    18721 cattgacaca gccagtcaag acaagcaaca tagccactcc catagccgag aatgcaaaac
    18781 gtttaataga tttcactgtt tttattcctt ctttaaaaat tatacctttc tattctactg
    18841 tttttttaca aaatatacaa tagttctgga gacctaattt gcgattttaa agtcagagta
    18901 gggaggaaag ttgcttagtt tgacatctaa gtagcttact tttgaaaaag gtgtcggtcc
    18961 ttttcggatt tcttggataa attttgccat gatagcagat gagtctgctt gggctaagat
    19021 ttccactgtg ccatcgtcgt tattccatac ccgacctgtg atgccaccga tttcaagggc
    19081 caggctataa acaccccaac gaaagccgac tccctgcacc ctaccttggg caatcattct
    19141 aaccttttgc ataccaaacc cctttgattg tgttataata tttctatgac tattataacc
    19201 tcaaaagcca attctgtggt aaaaaatgct aagaaattac accaaaaaaa ataccgcaag
    19261 tctgcctatt tgattgaagg ctggcacttg tttgaagaag ctgtccaagc tggagtgacg
    19321 attgagaaaa tctttgccct agaaaattat cgaaatcagt tagctgcttt tccgcaaact
    19381 gtctggattt cagaggatat tttgctggat ttggcggatt ctcagactcc acagggaatt
    19441 gttgccgtgg ttcaaaaaga agaagtagga caagctgatt tgagtcaggg caagttcttg
    19501 tttttggaag atgtgcaaga tcctggtaat gtaggaacta tcattcgaac tgcggatgca
    19561 gcaggtttta caggagtgat tgtttcagat aagtcggcag acatctacag tctcaaaacc
    19621 ctacgttcca tgcaaggtag tcatttccat ctgcccattt accggatgac tagtcaagcg
    19681 cttcttgacg aaactaaaaa ggtagctatc ccagtgctag caacaaccct atctaaagat
    19741 tctgttgatt acagagaact gccttctata gaaaattttg tactagttat gggaaatgag
    19801 ggtcaaggaa ttagtcccct tatggctgaa agtgcagacc agttggtcca tattagcatg
    19861 aaggggcagg ccgagagttt gaatgttgca gttgcagccg gtattttaat cttccattta
    19921 agctaatttt aacttttttt gttataatca aggaaagatg ttcacagaaa aggagaaaag
    19981 gatgaatcac actattatac atgaccgtgc aggtctcaat caattttacg ctaaggttta
    20041 tgcctttgtt ggtctgggaa tcggactatc tgctttggta tcaggcctta tgttgacggt
    20101 ctttcagtct cagttggttt acttgttgat gcaggggcgt ctctggttga ccattgctac
    20161 ttttgcggag ttggctttgg ttttcgttgc cagtagcatg gcttcaagaa atagtccagc
    20221 tgctcttcca gtatttttac tttactccgt tttaaacggc tttaccctta gttttgtggt
    20281 ggctttctat actccgggta cagttttatc tgcctttgta tcgagcgccc ttctcttctt
    20341 tgtcatggcg gcagttggta tttttaccaa gaaagacttg agtgggattg gtcgagcaat
    20401 gatggcagct ctcattggtt tactgattgc tatggtagtc aatattttct tggctagtgg
    20461 cttctttgat tatatgatta gcgtagccat ggttttggtt ttttcagggt tgattgcttg
    20521 ggataaccaa aagattcgcc tcgcctatga acaatcacaa ggtcgtgtag cgacaggctg
    20581 ggtggtgtca atggctctca gcatctatct tgattttatc aatcttttcc taagtattct
    20641 acgtatcttt ggtcgcaatg actaaaaggt ctatgaaatc agtgttttga gtgacactga
    20701 ttttttttaa attttctctt ttctt
//
LOCUS       DAVMEF010000019        20596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMEF010000019 DAVMEF010000000
VERSION     DAVMEF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.019"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(416..2143)
                     /gene="ezrA"
                     /locus_tag="VYQ30_000504"
     CDS             complement(416..2143)
                     /gene="ezrA"
                     /locus_tag="VYQ30_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1965666.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRILEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2225..4171)
                     /gene="gyrB"
                     /locus_tag="VYQ30_000505"
     CDS             complement(2225..4171)
                     /gene="gyrB"
                     /locus_tag="VYQ30_000505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1965667.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4186..4749)
                     /locus_tag="VYQ30_000506"
     CDS             complement(4186..4749)
                     /locus_tag="VYQ30_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1965668.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4834..5388)
                     /locus_tag="VYQ30_000507"
     CDS             complement(4834..5388)
                     /locus_tag="VYQ30_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1965669.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5399..6622)
                     /locus_tag="VYQ30_000508"
     CDS             complement(5399..6622)
                     /locus_tag="VYQ30_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1965670.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6659..9109)
                     /locus_tag="VYQ30_000509"
     CDS             complement(6659..9109)
                     /locus_tag="VYQ30_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1965671.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVFLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYFVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9309..10238)
                     /locus_tag="VYQ30_000510"
     CDS             complement(9309..10238)
                     /locus_tag="VYQ30_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1965672.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10378..10665
                     /locus_tag="VYQ30_000511"
     CDS             10378..10665
                     /locus_tag="VYQ30_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1965673.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10699..12033)
                     /gene="ciaH"
                     /locus_tag="VYQ30_000512"
     CDS             complement(10699..12033)
                     /gene="ciaH"
                     /locus_tag="VYQ30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1965674.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12023..12697)
                     /gene="ciaR"
                     /locus_tag="VYQ30_000513"
     CDS             complement(12023..12697)
                     /gene="ciaR"
                     /locus_tag="VYQ30_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1965675.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12806..15352)
                     /locus_tag="VYQ30_000514"
     CDS             complement(12806..15352)
                     /locus_tag="VYQ30_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1965676.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15475..15915)
                     /locus_tag="VYQ30_000515"
     CDS             complement(15475..15915)
                     /locus_tag="VYQ30_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1965677.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GDKSLAYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15890..16759)
                     /locus_tag="VYQ30_000516"
     CDS             complement(15890..16759)
                     /locus_tag="VYQ30_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1965678.1"
                     /translation="MKNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLDRILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16737..17237)
                     /locus_tag="VYQ30_000517"
     CDS             complement(16737..17237)
                     /locus_tag="VYQ30_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1965679.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17248..17946)
                     /locus_tag="VYQ30_000518"
     CDS             complement(17248..17946)
                     /locus_tag="VYQ30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1965680.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17939..18205)
                     /locus_tag="VYQ30_000519"
     CDS             complement(17939..18205)
                     /locus_tag="VYQ30_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1965681.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18271..19197)
                     /locus_tag="VYQ30_000520"
     CDS             complement(18271..19197)
                     /locus_tag="VYQ30_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1965682.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19272..20087)
                     /locus_tag="VYQ30_000521"
     CDS             complement(19272..20087)
                     /locus_tag="VYQ30_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965683.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPSAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20091..20411)
                     /locus_tag="VYQ30_000522"
     CDS             complement(20091..20411)
                     /locus_tag="VYQ30_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1965684.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMCPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6197 a         4529 c         3649 g         6221 t
ORIGIN      
        1 ctatttaccg tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg
       61 aactatccgt gagttctcta gtctggagat ttttcaatag acttcgttat tggacggtta
      121 ccaagttact caaagtacag atttgaagct gcagataaga ctgacgaaat cactttaata
      181 ctaaatgaaa atcaaaaagc aaactaagaa gctagcctca ggctgtactt gagtacggta
      241 aggcgacgct gacgtgattt gaagagattt tcgaagagta taaaacactg ttttgaggtg
      301 gtggataaat cggctgagta aaaactcaat ctcaggagaa aatgaagtaa atctccccac
      361 aactatagaa aaagatttga ttctgctcct cacacaataa aatctttttc ttttattaaa
      421 aacgaatcgt ttcacgtgtt ttctcatatg aggtaacaaa gcgattggtt acaccaggct
      481 ctgccacttc caatgcttga gaaatcttgt caaatgaagc gtgataatca aattcttttt
      541 caaaaatatc taaagcttcg ttaaatgctt cttgaatgcg ttcatcaaat gagcgatagc
      601 ggttagaata ttgcaagagt tgctctgtca aagttgcata ttgtacaata ttataagttt
      661 ccgtttctaa agcttccata tcattcgttg caatttcaag aattcgggta acagattcaa
      721 tgttaatcat tttttgttct aactcaacca ttaaatcctc ggtattattg cttgccgtaa
      781 agaataactt caagaaagtt tgtggaatac ctggcagatt gcgtttttcc atgtatcgct
      841 tgatagtatg gagacgattg acataaacat tggccttttg acgtgcatta atatcatctt
      901 tctcaatttg tgtcaggcgc tcactaactg aaatttgctc atcttcaata tcttttagtt
      961 gagtttgtaa atcctcaaga ttttcttcaa gaactgaata agcttgggtt ggttcttctt
     1021 gatttgaagt tacctcaaca atagctgcct caaaactctc taattctgtc tgaatacgac
     1081 gaacatggct tgcagctgtc tcaggaagta aataggtctt gttcaaacgt gcaatatctt
     1141 ctcccaataa agtattattc tctttcatat gttgaaggta agttggaaga gttgcaagta
     1201 gattttccac tactttctga gcagcaattt ctcgagtaaa aatatcatac aaggcattga
     1261 tttcctcttg tgcctgtcca ttctcatatt cggcattatc caattccaac tgacgaatat
     1321 tctcttggtt tttcttgaat gcttcataaa gcaagtggaa acgcgcttca atatccgttt
     1381 caacaaaatg ataattagca tcaattagtt tacgataacc ggcttccaaa tcctgtaatt
     1441 gatctggcaa ttctgtagaa agcgtcgtaa ccaaggctgg aacacgatcc acaatatgac
     1501 ttaaggccaa aatgtgattt tctgtattat ccaaaatcac tgcggcttcc acagggtcac
     1561 ccgatgaatt caaggttaca aattgtgaaa attcagattg gatattttct aattgttttt
     1621 caatttcatc caaggcttga ccatactgtt ctgaattttc agcaactcta tgctgaagtt
     1681 cctcaaataa atccaaagca tgaagaacac gaccactatt tttagattct tgcttctcta
     1741 agtctgccaa agcattgcga attgccgcaa tatcttcttc aatcaaagta atttgactct
     1801 caatttggtc aatttgatga ctggccttga gaaaacgaaa tgaatggtta taaccttctg
     1861 cttcaaagag attattttca atatcggcaa aagagttgag agataaatcg acccattttt
     1921 gattccattc acggaaagcc acttgacttt gtccaatcaa gtgcatattt tttacagctt
     1981 ctacttcatc atttactgga agattgtata gttcttcttt tctttcttct agcgcctcta
     2041 atctcccctc gtttcgctta cgtagaaaga ttgccactac ataagccaga actaaaatga
     2101 ctgcaattgc aaccattaaa taaattagtt gtccattaga catatcaaac tcctttttta
     2161 cttgaaacaa tcgtaacgat tatatcatat tttttagacc aagggaacta cttctcccta
     2221 ttttttagac atcaagtgta ctatagacag cattttcttc gataaactca cgacgaggct
     2281 ctactcgatc ccccatcaac atatcaaaga ttttatctgc ttctgcagca tcatctacag
     2341 aaactctagc catcaagcga tgttcgggat ccatggttgt ttcccacagc tgatgatcgt
     2401 ccatttcacc tagcccctta taacgctgaa tagtcggttt ggtacgacct tcactataac
     2461 gggctaaagc ttcttggagt ttgatttctt gatctgcacc cggctggata tattctttaa
     2521 tctcgcttcc aaccttgaca ccatagattg gtggttgggc aatataaaca taaccagctt
     2581 ctaggattgg tttcatataa cgataaatca aggttaaaag aagggtacga atatgggctc
     2641 catcgacatc ggcatcggtc atcaaaacga gtttttggta acgggctttc gaaacatcaa
     2701 attctgcgcc aaatcctgtt cccatggctg tgaaaagact acgaatttct tcgttagcta
     2761 gaatcttatc catacttgct ttttcaacgt tcaaaatctt accgcgaatt ggaaggatag
     2821 cctgaaactc acggttacga ccagatttgg ctgatccacc agctgagtct ccttcgacga
     2881 tgaagagttc tgtttcagca gggttattag aagaacagtc tgctagtttc cctggaaggt
     2941 tggaaatttc caaaccagat tttttacgtg tgacttcacg cgcacgcttg gcagccacac
     3001 gagccttggc agctaaaatc cctttttcca cgatacgttt ggcaatctgt ggattttcca
     3061 tgaggaaatc ggagaaggct tcactgaaga ggcgattggt aatcttgacc acttcgctat
     3121 ttcccaattt ggtcttggtt tgtccttcaa actgtggatt tgggtgttta actgagataa
     3181 ctgcagttaa tccttcgcga acatcttccc ctgttaaatt atcttcattg tctttcagta
     3241 acttattctt acgagcataa tcattgataa cacgagtcag tgccgtacgg aaaccttgtt
     3301 catgcgttcc accttcatgg gtatgaatat tattggcgaa actcatgaca ttttcatggt
     3361 aacctgttgt gtactgcatg gctacctcaa ctgtaatatc atccatctca ccgtctgtat
     3421 agattggtgt atcaaagatt acatccttgt tctcgttgat atattcaacg taactagcaa
     3481 tcccaccttc ataatgataa tgcttggttt gttccaaacc ttggcgctta tcagtgatag
     3541 aaatttgaag accgcgattt agaaaggcca actcttgaat ccgtttattt aatttatcaa
     3601 aatcaaagat tgttgtttca gtgaaaattt ctgggtccgg tgtgaagtga accgttgttc
     3661 cagttctatc tgtatcccca accacctcaa ggtctgcaac aacatgacca cgacggtatt
     3721 cttggtaatg aatcttaccg tttttatgga catgaacgtc taattgagtg gaaagggcat
     3781 taactactga cgaccccacc ccgtgaagac cacctgaaac cttgtatcca ccaccgccga
     3841 actttcctcc agcatgaagg actgtaaaga cggtctcaac agcaggacgg cctgtttttt
     3901 cctgaatatc gactgggata ccacgcccat catccacaac agtaatcgaa tcatctggct
     3961 caataaaaac ttgaatatgg ctggcaaatc ctgccaaggc ctcgtcaatt gagttatcaa
     4021 caatttccca gactagatgg tgaagacctt cttttgaggt tgatccaatg tacatccctg
     4081 gacgcatacg aacagcctct aagccctcta aaacttgaat ttgactggca tcataatcct
     4141 gtgcctgcag atttttgatt tcttctgtca tcttattcct ttttcttata tgtctaatac
     4201 ttgtcttaaa ttcacgatac tggtaaacaa gtgggtatca agtcctgcag cttgacctgc
     4261 ttcgatatca atcggccgat caccaatgac aagaccagag ctaatctgat acttttctct
     4321 taaataaagc atggattcgg gatttggctt tctcttaaag cctgagctag aagtcaccac
     4381 ttctgtaaaa taagctgcta tagaggtttt ttctaaaatt tccaaaacct gatcatttcg
     4441 atgagagacc aaaaaatgac ggccaccttg atttaaaatg tcttccaata ggtcagaaac
     4501 tccttcaaat aaaatcgggt gttcaagctc tctggcttca ttttccttgt acttttctaa
     4561 aaaattctct aaattgggag cgaatgtctc aatcgcaaaa ggagtagaaa cctttaaagc
     4621 ttgatagaca ctgtcatggt cttgtgtgat accatacagt gccaatgttt caacaaatgc
     4681 agctgttgaa gtttcataat tatccagtaa agttccacct aaatcccaga tgtaatcgtg
     4741 atatttcata cccttcatta tagcattttt ttatgaaaaa agcgatgatt tccctgagtt
     4801 ttccacataa aaaacgagag aataactccc gttttactga tttttaccaa agacatctcg
     4861 atagagcatt cctgttcgta aactctctgc gtcccctcct agttgctcca ccaactcgta
     4921 ggcaaaggca agggctgttg aaggaccccg actggttgtc aactgaccat ctaccactac
     4981 tgtttccttg acgtagtgac catcaaggat ttgctcttga acgccgtcat aacaagtgta
     5041 tcgcttattt ttcaatatct ctgcttgatt gagggcaatt ggtgccgcac aaatggctgc
     5101 tagtttcttc ccttcttgct cgaagctttg caattcttga atcaaggtct gattatcacg
     5161 taaatgtgca gaaccaggca tacctccagg aagaacaatc atatcatagt ctgataaatc
     5221 tccatcaaag acatgatctg ctcttacttg gattgcatgc gaacccgtta cttgctcttc
     5281 aaaaccaacc atatcacatg tgatattggc tcgacgcaag acatctacaa ctgtcaaggc
     5341 ttcaatttct tcaaagccct gagctaacat aactgctact tttaccatga ttttctcctt
     5401 atttaatttg ttttaataca acctttttcc gtttgaatgg gacttttccg ctcttttctt
     5461 cagctagatt agtctggtaa ctcatcgata aaagcaaacc aacaccaatc agattactga
     5521 taatagctga tcccccttgc gaaatgaaag gcaagggaat ccccgtcaaa ggaagtagtc
     5581 cagtcacagc accgatattc tcaaagatgt ggaagagcaa catcataatc aaacctgtgg
     5641 aaatataagt gtagaactgg ttatttgatt taagagtaat cttcaacata cggtaaatca
     5701 acatgagata gagggcaata accaggacag agccaataaa gccaaaatct tctgcaataa
     5761 ccgtaaaaat catatctgac tctcgaactg ggataagcag attcgaagca ttaaatccct
     5821 gaccaaataa gccaccactc ccaatggcaa tctgcccttg agcctgctgg taagtcgttg
     5881 tttgggcaaa ctcaaaggga ttgagccaag ccaaaatccg attgatttgg taggtcggca
     5941 ttccaatctg gtgaagaaaa gctcgtccgt ccttgctaat aaagatagct aagaaaccag
     6001 caactcctgt tacagcagtc acaaatactg ggataataat tttccaagaa acccctgata
     6061 ataaaacgat tcctgagaaa atggctacaa aaaccaaagc cgtccccaag tcactttgaa
     6121 gtgctaaaag aactaggact ggaatggtaa agagaatcat ccagaaaatt aacaaaaagt
     6181 ccagcgaaac cgtgcgtctc cattccttat gtttctttgt aaattggaca atgacacgag
     6241 ccaacatgag gatataggat atcttcataa attctgacgg ttgaaatagg gtaattccat
     6301 ttattgatac ccagtttttg gcacccgttg atgcaactaa gcttggatta taaaatacaa
     6361 tcggcaagat cataagtccc aagcctaaaa tatatagaaa gggggtcacc ttccaaagaa
     6421 attctgtatt aaagagcatg accacaaaac caatcacaag ccccaaggca atccaggcga
     6481 cctgctgccc taaaatgggc agaatattat tgggataatc atgactaacg gctatataga
     6541 tagccaccac accgatgacc agtagaaaaa atactggcaa gagcaaactg taatcgactc
     6601 ttgagtcgag agaacgtttc atatatacta accttatact ttcatacaat actatttatc
     6661 aaagttcatt aaaaaatcta tcaatagcct cgtcaacttc ggatcgagag atggttttaa
     6721 cagtcgcttc ttctgctaga gatgctacta tttgtttgcc gtatcgtttt ccgacgattc
     6781 tcctatccaa aataagagtt aaggaacgtt ggtattcacg tctcatactt cttcccaaag
     6841 cctgttttaa acgaataatg gccattggca attgataatc ataaaaggca tttttccctt
     6901 cttgattcag ttcttgatta atctttttcg tcaagggttc ttgaggattt tggaaaggaa
     6961 gcctcggtac aacttgaatc acaaaaggat ggcttgaaaa atcaactccc tcccagaaac
     7021 ttgctgcacc aagcaagatt tgttgttcac ctttttcaaa gcgtttcttt agctgatgaa
     7081 catccccatt tttatactgg gccaagtggc taactgtaag taaatccgat actgctagaa
     7141 gcatgtcttt agcggtaaag agaaccaaaa tcggttgctg gaaagcttga atttccacta
     7201 gtaaagcagc tacctctctg gcatagactt ctaaggaggt ttctgttacc agagggaaat
     7261 ctttgaccat aaccacttct tgttcctgtt ttccccgaga ttcaatcttg acaaatttag
     7321 cttcaggata gcctaaaagg tctgccaaag aaaccctctg actaatctca agagtagccg
     7381 atactcccag gacttggcaa ctctcaggaa gtaaagagga cagaagagta cggcctgatt
     7441 ttgtagaaga aatctgaatt ttcttttgac tcgtttcagt tacttcaagc cagtaatcac
     7501 cttcagctgt aaaatagcga accagctcat caaagccttc tacttctaat tctgaaaaat
     7561 actgatggag attgtccaga gaatctaaga tatttttcct agatttacca gactgaaatt
     7621 gttctatcaa gtagagacac tcaaagcgaa tactttctag tatccgttgt tgaaccctgt
     7681 tttcttctcc tactaaagcc ttatcaatta aatcgataat agattgtata tcgtaggtct
     7741 cttgaagcag attttctaga gctaacaaaa tcttttggac ttcatcaata atcagtaaac
     7801 ggtcactgac aaattcagga ttatcttcaa gtctggtcac gaaataggca tgattagtca
     7861 ctaaaagctt gcaagtctct gccctttctt gactacgttt ccaaaaatct tccgtcacaa
     7921 ataagctctg ggatgataaa ttcccatcat gacgaaggtc tgctagaaaa tgttggtaac
     7981 ggtagagttg cccgatttca tccaaatctc ctgtctctgt ctcagtaagc cagaccaaga
     8041 cttgcatttt aaagcgtcta aataagcgat tttcatcatt ttcctgcaag gaatgataaa
     8101 aggcatccaa cttcagataa ttttgtggtc cctttaagct atgaatatct gtatggaaca
     8161 cttccttgag acgtttacct tcttcttcca tgatttgatt ttgaagaatc tttgtcggaa
     8221 cactaagaac aatttgtcgc tctttggatt gagataaagc gggtaagaga tagccatagg
     8281 ttttcccaat ccctgtcggc gcttgaatca gaaagacagg ttcatctttc aatagcaagc
     8341 caacctcttt agcaaaactt tcttgttcct ccctcacttc aaggttcaac agagaaatat
     8401 ttttagaaaa gtcttgagat agttttcgta gctccagaga agctgccgtt ttcttaaaat
     8461 acagaccttg aacttggacc aagtctggag aactcaggat agattggttg cgataagttt
     8521 cctcaataac caggtaggac tcatatagga gagcgtcagc catttccagc aagcgttcca
     8581 agagaccttt aggaagctgg gtcatctttt cccgtaaaaa aagaagtaat tctgctgtag
     8641 cttgggcatc tgaaagggct gtgtgtgcgt gtttaagagg aattcctaat tctcgacaca
     8701 aaatcggcaa gctatatttt tccagttcag ggaaaaagac ctgggccaat tcgaccgtat
     8761 caacacgagg gtttcttagc tcatagcctt caaaaaataa attttccgcc aagagattag
     8821 catcaaactg aacattatgg gctacaaaaa tcccatcctc caccaagtca aatatttttc
     8881 tggcaacttg cgaaaaatca ggtgcttgcg ccagacgttg gtctgtcaat cctgtcagtt
     8941 ctttgatatg agcatccaag ggttcatgtg gattgacatc cgtcgtatag tgatcgacga
     9001 tttctccgtc ctcaatcacg acaattccca cttggataat tttagcctta ctacctgtgc
     9061 tagttgcctc taaatccacc acaacatagc gattaccaat cgttttcatt ataaaaaatt
     9121 ataccaaaaa aagggccatt tggcacctgc aaacatagga taattttcaa actcaagaac
     9181 actacttttc ttaaaaatcc catcgaatag agcattctag cctgctgatt tttgtaaaat
     9241 agtgaagaaa tacgaaaaat tgagagaaga aatgtttgca tcttccttct ctcaacgaat
     9301 catgtatttt atctaaccat gtcaggatcg tagtcataaa atcctgtatc aaggaaacgg
     9361 tttttagggt gaagtttacg aacttcttca tccagcaaga aggcttgatc atggctaaag
     9421 ttgccctctt gaatcaaatt acgcgcttgg ataaagagtt ttgcaatctc aacaaagttc
     9481 tgaccaggag tgtagagccc ttctagtttc cagcgagtaa agccatgctc caccaattct
     9541 gttaatttga tcatcaaatc aaggtcattg ttggcaaaga tatgggtccc atgattatct
     9601 tcaaaaatgg aatagtggct ctctggatca cttggctcag ccaagaagag gtcacgttta
     9661 tgcgtctttt catcatcgat atgtgtaaag ttatagtagt tttgcaagag tggacgttta
     9721 gaatgatgga tgacgctagc accgtaaacc aaaacttcag caggaatttc caaaatctct
     9781 ggcattttga aaagttcagc tgatggaatt tcacgcgcca aaacagcctc agatgcgcca
     9841 gccttttgtc cccagaagtt aatctgacga ctgctagtta ccatggttga agcatcgtag
     9901 atggtcttaa atgaataacc atcgcggtta actacgtaaa agacgcctgc atccccaatc
     9961 gtaatatagt ctgtcttgat ttcttccaag aagtctaaga aaggcttgat acggtccatc
    10021 atatcttggt gcatgagagc attgaccgca acgagcaatt ccttaccagc atcatgaacc
    10081 aacttagcga tttcacgtaa ttggtcataa ctaaaggtcg ttggcagacg aaggccaaaa
    10141 tctttctcac cgacatagat acggtctacg ccagcttcga gtagttgttc aacttgttca
    10201 atactttcag cagttgctgt aatgacaatc ttttccataa gaaaaattat accacatttc
    10261 tccaaaatca tccatgcttt gaaaatgaaa agggacttta ttctttttgt aacctttctg
    10321 taataattct ctgccgaaaa aatgataaaa taggtatgta ctgttaagga gagaattatg
    10381 cccgtaagaa aattacaatc ctatgaggta gactatcaag aagaattaaa ccagcagctt
    10441 cctcattacc aagcttatac acctgaagcg caatctgatg ccaatctcaa ggaaatccta
    10501 ttttttatta atatcgctgt tttttgtatc tgtattgcta tctttagttt tatcttttta
    10561 gcattaaaat tatcaactgc tcttgccttt gccgcagcaa tcggattcag cttacttgtt
    10621 ttaaaagttc aacggtctat tatcaaacga aaacgtagaa gataaatcca aaatatcgcc
    10681 ccaattggag cgatattttt attttttctt tttagatggt gtctgaatgg caatcttcac
    10741 ttcaaagatt gttcccttgg gtttattatc tttgacagta acagttcctt ttagagcatc
    10801 tacaatttgc ttggctaggg ataatcctaa accaaaacca cctttttgcc gggttctagc
    10861 cttgtctact cgataaaaac ggtcaaaaat tttcttttta tcttctgtcg aaataccgat
    10921 tccattatca gaaacaagta aataaagatt gcgatcggtc gccgagataa gaaaatcaat
    10981 ttcaccatcc tcctcagtat acttgacggc attatcgaaa agaatggtca tcagttgttt
    11041 cagaagaagc tgatctgtga caattgttcg atggatacga ttttcaaaac ggaagacacg
    11101 attattttcc gaagcaatca tctcatagtt tgtgaaagtt gtattaaaaa agctagttgg
    11161 aacttctgca agctccggct taatcccatc atctctccga gctaagttca gcaagcttgt
    11221 cgttaaaaaa cgcatatttc ggacttcttc caaactcgat gcaatgcttt cgctcacatc
    11281 cataatggta gcttctggct tacgaaaaag ggtctctaag cgattttgca aaactgcgag
    11341 tggagttcgt aactcatgac tggcattttc cacaaaagac tgttgcttct gcatactctc
    11401 aagcaggggc ctaacactga ccctagctag atagagactg gcaagtaaag acaaaatcca
    11461 gaaactagcc atcacgacca caatcaattg ctcatgcttt tgactagcct gctccaactg
    11521 actggtatta atcaagacag cagcatactt gatattggtt gaaaccgaac taatattggt
    11581 ctccatcaaa atcacacgat agatttcttc ctgcccatag ctattaaaaa cctgaatctg
    11641 gtagatatgt cctagttctt tcttttctaa cttaatctta tccaagccca aaaatcgatt
    11701 tccagaaaga agttgagtaa agtctttatc aaagagaatg acttccgtat tggaactgac
    11761 attaggtttt atttctactt tactagcgtc cgccctagca ttttctaaat ctttaatctc
    11821 ttctgttgcc ctatttatag ccagctgaat aactgcttga ggattttcac tcagtccatg
    11881 aagcttatcg tccaccgaag tataaagact cgaatgcatg acttgtaaaa taatcagagt
    11941 cattgtagaa aaaatcaggg tgaagacacc gaagttgcgg ataaaataac taaagtcatc
    12001 cgcataccat gtttttttaa gtttactgaa catcttttaa aagataccca acactacgca
    12061 aagtttgcaa attctctgca aaagtggttc cctttaattt cttacggact tttgaaacat
    12121 agacttcgac aaccgaaatc gttgtatcac tatcaaatcc ccatagacgg tcaaaaatct
    12181 gcgtcttagg caaaatcaca ttttgatttt gaaggaaata aactagtaaa tcgaactctt
    12241 tccccagcaa ttcgacagga gtatcttcaa ctttaacggt attggttgat aaattaacca
    12301 cgatattccc ataagtcaag gtgttttcat taaacttccc tgaacgttta agaagggcct
    12361 gaatccgcat tttaagttct tctaggtaga aaggtttggt cagataatca tccgctccca
    12421 gttcaaatcc atgtcccttg tcatccaaac tttccttggc agtcataatc agaactggtg
    12481 tcgtaattcc cttttcacgc aattctttta agacttggaa accatttttt tctggcaaca
    12541 tcaaatccag caaaatcaag tcatagacac cactctcagc ttcgtagaga ccttcttctc
    12601 catcaaatac ctgcataaca tccgcaaaat cgtctaaaaa gtcaaatact gaatttgaca
    12661 gacctaggtc atcctcaacc aataagattt ttatcatgag aaactcctcc ttattaaaac
    12721 tattatacca aatttgcctt aaaaaaaact caactctctg cattttacat gagatagctg
    12781 agttttcttt ttattttagg cttatttatg catttccgta ttgaagaaca actgcttcga
    12841 ctgcagcttt ttcacggcta atcaagtcaa cacgtgctgc aatttccttg attcccatac
    12901 cgatgttacg gctaagagca aggtcagaaa gttgcggctc aaagaactcc ttgtattccg
    12961 ccaagcgttg ctgagtctta aatacatgag caggaaggat aacaaagcta tcaaagctca
    13021 tatctcctcc aagggctgcc ttaatccaag cccagttttc acgcgcccaa gaccaagctg
    13081 ttttctgagt tgcttgatga gctaggaatt ggtaatacca agcagacaag tcctgtggtt
    13141 tgaccacaaa tttgtccttc caagaagtaa tcaagttttg gatattatcc gcatctgtac
    13201 tgtatgcaag agctgctgcc aactggcgtt taaagacagc atctgttgcg tgagtataag
    13261 tatcaagata aagtgctaac aagtctttag tctcatgatg tttcatctca ttaatcagaa
    13321 cttgtgaacg aatagctgct gggagttctg caagattctc cttgtgtgtt gcgaagattt
    13381 ggctagcgac ttgactagct tctgcatcat ttgagcgaat catcatcgaa acagccagct
    13441 gacgaaccaa ttcatcctca tctgattctc cgtctttagc ttcaaaacca agacggtcat
    13501 agttatgacg agccaattta gcaaccagtc ctttgaaggc tgtttcagca tccgttcctt
    13561 catcaataaa gcgctcaagg gctgaaatca cttgagaaac agctgaaacc accagataag
    13621 actcttcctt agcaagttta tcaagaactg gaagcaagtc tgcataagaa atgtgccctg
    13681 cctcagccaa caaacgacgt tcttgaacaa tttgcagttt gcttgtgtta tcaagtgtct
    13741 ctagctcagc aagaacagct tctaacaagt ctccttgata gtcggtaata tagtgggcag
    13801 tattttcagt gttgagacga agagctcctt cattctcagc aagaagagct gcgtagccag
    13861 ggatttcgat actttcagtt tcgagtgtat caggcaagcc tttccagttg ctattgaggg
    13921 gcaccaccca gagacggttc ttgtcttcgt tctcaccgat gaagaattgt ttttgtgaaa
    13981 tcttcaagac atcattttca actttgacag taagaactgg gtaaccaggc tgttccaacc
    14041 aagaatccat gaaggctgcg acatcacgtc ctgacgcttg accaagggca tcccaaaggt
    14101 cactaccaat ggtgttgctg tattggtgtt tttcaaagta ggcatgcaaa cctttagcaa
    14161 aatcagcatc tcctagccaa cggcgaagca tgtgcatgag acggcttcct ttggcataga
    14221 cgatagcgcc gtcaaagagt gtattgattt catctggatg tttaacttcg acgtggacag
    14281 actgaacgcc atcagtagcg tcacgttcaa gagcaagagg tactccacct gtttggaaat
    14341 cttcaaagat attccagctt ggttcgatgg tatccacaca gacgtattcc atcatattag
    14401 cgaaactttc attgagccaa aggtcatccc accatttcat agtcacgagg ttcccaaacc
    14461 attggtgagc caattcatgg gccacaacaa gggcaacttg ttgacggcta gcaaatgtag
    14521 agttctcatc gacaaccaag taaacttcac ggtaggtcac aagaccccag ttttccatag
    14581 caccagctga gaagtcagga agggcgatgt ggagagattg aggaattggg tacttaactc
    14641 catagtaatc ttcgtaaaac tcgatagagc gaacagcgat atccagtgag aaatcaagat
    14701 ttgaaagtgg atgtgctttg gttgagtaga cacctaccag ggtaccattt ttagttttag
    14761 cggtcacccc ttgcaaatca ccagcaacaa aggccaacaa gtaagaagac atgcgaggtg
    14821 ttgtctcaaa cttccagata cctgtttcct tacggttttc aacatcgatt tctggcatgt
    14881 ttgacaaggc caattcacct tctgcttggt caaagcgaag agagaggtca aaagttgctt
    14941 tggcttcagg ctcatccaca catgggaaag cttcgcgcgc aaaatggctc tcgaactgag
    15001 tagacaagac ctccttcttg actccatcaa ctgtataata agaagggtaa atccctgtca
    15061 tgttgtctgt aattttacca gaaaaggcaa gaaccaattc aacttgacca gcctcagcca
    15121 attcgatatg aagggcttca ttgtcatggt caactgtaaa tggacgagct tgacctgcaa
    15181 cttctacaga ggtgatttcc aaatcttttt ggtggaggga gatgcggtca ctctgtgctt
    15241 gaccagtgat ggtcactttc ccagaaaaag tcttggtctc acgactcaaa tctaaaaata
    15301 aatcataatg ttcaggaaca aattgcttaa taaaatgttc aactgcttgc atagttttct
    15361 cctattctaa gtttaagagc tgctgctctt cttcaaacaa acttattata tcatattttg
    15421 cttgagaaaa aaggattgga cagcgatttc caaaactttc ttccttctaa tagtctacag
    15481 tgggtagacc ttgcgaaatt cttctaaaac aaccttgctt tcaggtgtaa aagtcaatcc
    15541 tgcttcaccc aagcagtctc tgaaaaagtc ctcatgaacc tggcgccaat aggcaagtga
    15601 tttgtctccc tcaccttcct tataggcatg gtcagctgac actcgattga agggtttaac
    15661 agaaaccttt gtaatttcga caatgcagac agcctgattt tgactatcta aaatgacatc
    15721 gaaggtccct acttggggaa gtggttcgtc ttctagtaca tagaggtcat aggctgatgc
    15781 tgttgctgtc ttttctcctt taaacaccaa atccgctaaa aggtctggtt caactccaaa
    15841 agcccaggca tcgatttcat ctccgatcaa aggattgatt tgcttgtatt tattccacat
    15901 ttcttgcggt gtcatgggtg ctcctttgta attttttact ttcttctttt atgtgtttaa
    15961 gatgatctgg atggtcaatc tctaaatcaa aaatctctgg aatagaactg tagtggataa
    16021 tgcacttgat acccaactga ttcatttttt gtatgaaaga agtattcaga tagcctgcta
    16081 cagcaaaatc aatcttgttc tttcttgctt tatcctgcat atctcttagc atatctaaca
    16141 ttattggact ttccatatca tgccattgac tgtttctcat agtcgcaaaa acaaaggaag
    16201 tcaaatcatt cattccaact acaatctttg aaatgcccgt ttccagtatc ctatccaagt
    16261 caaaatacgc tgacggtaat tcaatcatcg ttccgacttt cccagtaaag ccatactgac
    16321 gcaatactgt aatagcttgt tttaattggt cggcatcatt gacaaaagga aagataagag
    16381 atagattggg attggtttga taaacttctg taacgacatg tgcttcagcc tgaaattcat
    16441 ccaaacacgc cagtaaacgc ctggttcctc tatagccaaa caagggatgc ccttcgtcaa
    16501 aaaactctct agtccccact aaacaattgg cttctgtatt cgttaattca gaaaaacgat
    16561 accaaacttc ttcatctgag tacagggagc agatagtctc tagataatct tttacaaatt
    16621 gctgacaact tgctaatatg atgttttgat taagctctct caacaaatat tccccacgaa
    16681 tcatgccgac gtggtgaaat agttgaggat aaattttttc aagaattttt tcaccactaa
    16741 gggcaagttg atttttcatc attcaccttc caattcatgt aagaagtctt gtccagttct
    16801 ggaaatccta ataattcaga cttaaccttc aagactaatg gcgatgcatt ttcttctgta
    16861 atctcttgaa tatccatcca aatatatcca agtgaatcat tcgcaccatc agacacagct
    16921 tccgaagtcg taacttgagg tgcactctca ttcatttcaa catcatacaa ggccatgaca
    16981 tggtgaacca taaaatttgt taactcttct ctgacgaaaa catcgtagat tcgaggatta
    17041 gagtagcttc taacagtaaa tcccgtctct tccataactt ctctagtcag cgtttccgtc
    17101 agtccttcac caagttgctg actgcctcca ggtagatcat accgatgttg ataagggcct
    17161 ctcgtttttt caatgcagag taacttccca ttttctaagc aaacagcgta gactccaaag
    17221 tgttttttga tttccatcca actcctccta cttcaaagac cagccaccat ctattgtcaa
    17281 gatttgtcct tgcatggcgc tcgcttttcc acttgctaaa aaaagactaa gctctgctat
    17341 ttcctctggc tcaatccagc gcttgattgg agtttcacta gccacccagt cagccaaacc
    17401 acctggttca aaatccgcag cggtcatagc tgtcttgact gctcctggag cgataccaaa
    17461 gacctgaatc ccagcttcag catagtctag agccaactgc ttggtgaagc cagccaaggc
    17521 atgcttggat gaagtatagg cgtgaccacc tccacctgct aggctagaag caatggaaca
    17581 catattgatg atgattccct ttttattttc cagcatttgt gtcaaataat accgagtcaa
    17641 ctctactgga ataatgtagt tgatttcaaa aatctcttga atgtcctgcg ccgtttgttc
    17701 caacagtggt ttgtaatcat ccaaaactcc agcagtatta cacaaaacat ccacctgagg
    17761 gcaccagtca aaaataggtt ccaagtccaa ggtcaaatct ctctgtaaaa agcgaaaatc
    17821 accctctaag agtggctttt caccttggtc aactccataa acttgatagc ccttctctaa
    17881 aaagaggcga gcttgagcca atccgatccc tgaactcact cctgtaatga gtacacgttt
    17941 agtcatgcac ttctacccaa tccgttgcca aaacatcaca aactgtcggg ctccacatgg
    18001 aaaaaccttc tccttcgcca gaaacgttga ttaggaaata aggtgtcatt tcaagtgcaa
    18061 gcccattttg ctcgatggta tcaaagagtt ggacatagtt ttccgcacct ccccaaccag
    18121 ttcgtacata ttttctttta gcctttaacc caggcaggat ctcttcaaat gtcatgtttt
    18181 tctcctttaa ttctacattc ttcatttaat tatagcaaaa aaccgcttta tacggctttt
    18241 tgaatgtgag ttattcaaac ctgctactac ttacggcaaa ttattccctg cagcaagata
    18301 aatttcatac cattcttttc ttgttaagct aaagtttgcc gctcggctaa cttctctcaa
    18361 gtgcttagga tttgttgtac ctacgactgc ctgcattttt gctggataac gcaatatcca
    18421 agaaatggca atagttgaag aggttactcc atatttaata gctaaacgat caagtacttg
    18481 atttaaagct tgaaatttct catttccaac aaaattccct ttaaaatacc cgaattgtaa
    18541 gacagaccat gcttgaatga ccacatcgtg taattggcaa tattcaaaaa tgctgccatc
    18601 tcgcatagct gcttgactat cttccatatt aacatgaaaa gctgattcaa atcctggagt
    18661 aaaagccgca ctcaattgta gctgattaac agctaacggc tgcttgacat cttttttaag
    18721 caactccatc atcataggat tttgattaga aactccaaaa tctcgaactt taccttgttt
    18781 ataaaggaga ttaaaggctt ctgctacttg gtcagattcc atcaaagcat ctggtcgatg
    18841 aaggagcaag ctatctagat gatcaatctt caatctttgc aaaataccgt ctactgattt
    18901 tataatatag tccttagaaa aatcaaaata ggtaaattct tcaatgcgaa tgccacattt
    18961 ggactgaatc cacatctttt ctcttaaatc tggacgattt tttaggacaa gacctaacag
    19021 ttcttcacaa cgaccacgac cataaatatc agccaagtcg aaggcattga ttccaacaga
    19081 aagtgctgtt tctacaagct cttcaacttc ttttacagat ttatctttta ttctcatcat
    19141 tccgagaaca atttctgata attctttgtc atcttgacca agagttatgt atctcatcaa
    19201 atttttctcc tttaatttct aacattcttc ccttcattat aacaaaaaac cgctttgcaa
    19261 cggctttttg actatacttc actccatttt atcttcttaa acccacggaa caagacaaag
    19321 attccaataa agaggacagc tgaaggaata acttttgtaa ggaaaacatt tgaaattccc
    19381 atccactcat aataacggag cagagaaccc accacaagat gggcaataat catactgaca
    19441 aatggacgaa agaccgcttc tttccaattc caaataccga taactagcga aatcgtaaag
    19501 acagacaaac tatcccaggg agccggaata taaaaggctc cttcttgtat gaagcttgcc
    19561 attcctacat atcctaaaac aactagaaga actatagtcc caacaacaat gtaagtgcca
    19621 attttcattt taggagaatc ttggactaaa cttcttcgta aaattgtggc cacaagtcca
    19681 aatccaatca gaaaaataag aagttgccct aaaaatgtga gcaaattgac tgttaagaga
    19741 ggacctttag aaaaatcact tagtagttga taataacgta ataccgccag gacaagaatt
    19801 ggcgtcaaaa gggactcttt gatagaactg cgaggtgctc ccttgagaat ctctttcatt
    19861 atttttttag gattcttacc tagataatcc tctgcactca tgccatctcg ttctgcttct
    19921 gagaaatcta gcatcatcaa atagatctgc tctctgagat agtcttcatc atagagaaat
    19981 ccagcaagat taaaactttc ccacaactcc tcaaaatact tttgattctc ctcagaaaac
    20041 tcatgtagca aagcgcttgt ttcttcgtaa tacttcattt tcttcatggt ttaaccccca
    20101 ttcttaatcc cttctacttt ttgactcaaa tcgtcccatt gttgccaaaa gactgagaca
    20161 cgctcttctc cttctttcat taatgaaaaa tacttccgat ctggaccatc tggcgacggg
    20221 cacatgtcgc ctcttatcca ttgatttttt tctaactttt gcaacaaagg ataaatagtt
    20281 cctggaacga tagtatcaaa tccagcctct cgcaaagtct gaaccaactc ataaccatac
    20341 cgctcttttt gaccaatcat atccaagaca caaccttcaa gaacaccttt taatagctga
    20401 gtttctttca tcacttctcc cttctaatct attttgtaat acctactagt aacttcacct
    20461 atagtatatc acttctacac tagtttgtaa agcataatag ttaatactct tcgaaaatct
    20521 cttcaaacca cgtcatcgtc gccctaccgt atgtagggtt actgacttcg tcagtttcat
    20581 ctacaacctc aaaact
//
LOCUS       DAVMEF010000020        20377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMEF010000020 DAVMEF010000000
VERSION     DAVMEF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.020"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            41..385
                     /locus_tag="VYQ30_000523"
     CDS             41..385
                     /locus_tag="VYQ30_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965685.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIINGIVNGLIGRYIKLGIY
                     AAKYGISMVRSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            672..1316
                     /locus_tag="VYQ30_000524"
     CDS             672..1316
                     /locus_tag="VYQ30_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1965686.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            1489..2406
                     /locus_tag="VYQ30_000525"
     CDS             1489..2406
                     /locus_tag="VYQ30_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1965687.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            2424..3308
                     /locus_tag="VYQ30_000526"
     CDS             2424..3308
                     /locus_tag="VYQ30_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1965688.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(3324..4175)
                     /locus_tag="VYQ30_000527"
     CDS             complement(3324..4175)
                     /locus_tag="VYQ30_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1965689.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            4375..6432
                     /gene="pbp2b"
                     /locus_tag="VYQ30_000528"
     CDS             4375..6432
                     /gene="pbp2b"
                     /locus_tag="VYQ30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1965690.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            6443..7039
                     /gene="recR"
                     /locus_tag="VYQ30_000529"
     CDS             6443..7039
                     /gene="recR"
                     /locus_tag="VYQ30_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1965691.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            7215..8258
                     /locus_tag="VYQ30_000530"
     CDS             7215..8258
                     /locus_tag="VYQ30_000530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1965692.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            8342..9715
                     /locus_tag="VYQ30_000531"
     CDS             8342..9715
                     /locus_tag="VYQ30_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1965693.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLFLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAVNGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENEAK"
     gene            9702..10313
                     /locus_tag="VYQ30_000532"
     CDS             9702..10313
                     /locus_tag="VYQ30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1965694.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDVEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            10404..11108
                     /locus_tag="VYQ30_000533"
     CDS             10404..11108
                     /locus_tag="VYQ30_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1965695.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            11327..12700
                     /gene="ftsA"
                     /locus_tag="VYQ30_000534"
     CDS             11327..12700
                     /gene="ftsA"
                     /locus_tag="VYQ30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1965696.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            12717..13976
                     /gene="ftsZ"
                     /locus_tag="VYQ30_000535"
     CDS             12717..13976
                     /gene="ftsZ"
                     /locus_tag="VYQ30_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1965697.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVTPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            13981..14652
                     /locus_tag="VYQ30_000536"
     CDS             13981..14652
                     /locus_tag="VYQ30_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1965698.1"
                     /translation="MNVKENTELVFREVAEASLSANRECGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            14662..15201
                     /locus_tag="VYQ30_000537"
     CDS             14662..15201
                     /locus_tag="VYQ30_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1965699.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDFLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            15201..15464
                     /locus_tag="VYQ30_000538"
     CDS             15201..15464
                     /locus_tag="VYQ30_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1965700.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            15461..16246
                     /locus_tag="VYQ30_000539"
     CDS             15461..16246
                     /locus_tag="VYQ30_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1965701.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            16255..17043
                     /gene="divIVA"
                     /locus_tag="VYQ30_000540"
     CDS             16255..17043
                     /gene="divIVA"
                     /locus_tag="VYQ30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1965702.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            17391..20183
                     /locus_tag="VYQ30_000541"
     CDS             17391..20183
                     /locus_tag="VYQ30_000541"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1965703.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMNILSQVSETYRKIRNTLRFLIANTSDFNPDQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCVSI
                     VEENFAEAVAEGFEEK"
BASE COUNT         6076 a         3734 c         4621 g         5946 t
ORIGIN      
        1 tctttgtgag gtagagaaat gagaataaaa tatttaaaaa ttggtatctt ctaagtatgc
       61 tgcaagagcc agtttcttag atggacaggg gattacagtt gatgagatgt cctggataat
      121 taatggcatt gtgaatggat tgattggtag atacataaaa ttaggtattt atgcagccaa
      181 gtatggtatt agtatggtgc gatcgatctt aagtagggca gctgcaactg cggcagcaag
      241 agtaggatta ttgaccaaga tttttggatg gattttacga atagctgtgc tcgcagctga
      301 tgtatatggt aattttgcca acaatattgc tgtagcttgg gatgcacatg ataaaatccc
      361 taacaatggt cgtataaact tttaaaatgc gagaatgaaa gcactttgta tttttttatt
      421 gaatatgtta gcttggacag tgcttgcaat gacagtccgt ggagggctag atggatttaa
      481 tgagcatact tggagtacta ttttaattgc ttcgctgtcc gggatatata tagattatat
      541 gtccatagat aaaagtagaa aaaagtccaa aagaaaagag aaaaaataga tttgttcatg
      601 gtagggactt atgaaagctt tactgacaaa aaagaaaaca gtttacaaag aaaaatgatg
      661 gaggagcaaa catggcacaa aaaggagtaa gccttatcaa ggcagcattt gatacagaca
      721 actttctcat gcgttttagt gagaaagtct tggacatcgt gacagccaat cttctttttg
      781 tcgtctcttg tttgcccatc gtgacgattg gagtggctaa aatcagcctc tacgagacca
      841 tgtttgagat taagaagagc agacgggtgc ctgtttttaa aatctatcta agatctttca
      901 agcaaaatct gaaactaggt cttcagctgg gtttaatgga gttaggaatt gtgtttctta
      961 ccctttcaga tctctatctt ttctggggtc aaacagctct gcccttccaa ttgctgaaag
     1021 ccatttgttt aggtattctg atttttctta ctatcgtgat gctggctagt taccctatcg
     1081 cggcacgtta tgacctatct tggaaagaaa ttcttcaaaa aggattgatg ttggctagtt
     1141 ttaactttcc ttggttcttc ctcatgttag ccattcttgt cctcattgtg atggttcttt
     1201 atctgtctgc cttcagtcta ctcttaggtg gctcagcctt cctacttttt gggtttggac
     1261 ttcttgcttt tttacaagtg ggagtgatgg aaaagctatt tgctaagtat gagtaagggg
     1321 tgcaattatt tctgaaacta ctttcaaagg ctccaaacgc tattctataa gcgagaaact
     1381 aaaatctaag tgtctataag atattgaaag ccttttcggt aaagtgtata ctaaagaagt
     1441 aaagaaaaat ctgctttagc agaaaataaa aataatagga gaaaatctat gtcagatttg
     1501 aaaaaatacg aaggtgtcat tccagccttc tacgcatgtt atgatgagca aggagaagta
     1561 agtccagagc gtacgcgtgc cttggttcaa tacttcattg ataaaggtgt tcaaggtctt
     1621 tatgtcaatg gttcttctgg tgaatgtatc taccaaagcg ttgaagatcg caagttgatt
     1681 ttggaagaag tcatggcggt agccaaaggt aaattgacca ttattgccca tgttgcttgc
     1741 aataatacta aagatagtat ggaacttgct cgccatgctg aaagcttggg agtagatgct
     1801 attgcaacga ttccaccaat ttacttccgc ttgccagaat actcagttgc caaatactgg
     1861 aacgatatca gttctgcagc tccaaacaca gactacgtga tttacaacat tcctcaattg
     1921 gcaggggttg ctttgactcc aagcctttac acagaaatgt tgaaaaatcc tcgtgttatc
     1981 ggtgttaaga actcttctat gccagttcaa gatatccaaa cctttgtcag ccttggtggg
     2041 gaagaccata tcgtctttaa tggtcctgat gagcagttcc taggaggacg cctcatgggt
     2101 gctagggctg gtatcggtgg tacttatggt gctatgccag aactcttctt gaaactcaat
     2161 cagttgattg cggataagga cctagaaaca gcgcgtgaat tgcagtatgc tatcaacgca
     2221 atcattggta aactcacttc tgctcatgga aatatgtacg gtgtcattaa agaagtcttg
     2281 aaaatcaatg aaggcttgaa tattggatct gttcgttcac cattgacacc agtgactgaa
     2341 gaagatcgtc cagttgtaga agcggctgct gccttgattc gtgaaaccaa ggagcgcttc
     2401 ctctaatcta aaaggaggta tttatgacat actacgttgc aattgatatc ggtggaacca
     2461 acatcaagta tggtttggtt gatcaagagg ggcaacttct tgaatcgcat gaaatgccaa
     2521 ctgaggcgca taagggtgga cctcatatct tacaaaagac caaagatatc gtagctagtt
     2581 atttagaaaa aggcccagta gcaggtgttg ccatatcttc tgctgggatg gtggatccgg
     2641 ataagggtga gattttctat gctgggcctc aaatccctaa ctacgcaggc acccagttca
     2701 aaaaggaaat cgaagaaagc tttactattc cttgtgagat tgaaaatgat gtcaactgtg
     2761 caggtcttgc tgaggcagta tctggttcag gcaagggagc aagtgtgaca ctttgcttga
     2821 ccattggaac cggtatcggt ggttgcttga ttatggatag gaaagtcttc catggtttta
     2881 gcaattcagc ctgtgaagtc gggtatatgc atatgcagga tggagctttt caggatctcg
     2941 cttctacaac agctttagtg aaatatgtag ctgaagccca tggagaagat gttgatcagt
     3001 ggaatggccg acgtatcttt aaggaagcta ctgaaggaaa caaaatctgc atggaaggta
     3061 ttgaccgtat ggttgactat ctaggaaaag gtctggcaaa tatttgttat gtggccaatc
     3121 cagaagtggt tattcttggt ggtggtatca tggggcaaga ggctatcctc aaacctaaga
     3181 tccgcacagc cttgaaagag gcattggtac caagtttagc agaaaaaaca cgattagaat
     3241 ttgcccatca ccaaaataca gcagggatgt tgggtgcata ttatcatttt aagacaaaac
     3301 aatcctagtt tggctcagcc aaactaggat tttcttacac gtttttgtct acgatagccg
     3361 ttgagttttt tattttccca gtagctatta aagatttttt ccttgctttc gcgattgatt
     3421 tccaaaaagt aggcataaat caaatcgata aagaagagca taggaagttg agcggatatt
     3481 cgttggatgt aggagggttg gctgtgggtg gctacaagaa cagtctctgt ataggtctgg
     3541 ctatttttat tgggaacact tgtaaagagt acagtctttg cccccatctc cttggcatct
     3601 aatagactat ctaaaataga aggagttgag cctgaaagtg agaagcccag tactagacaa
     3661 ttttcatcca tgatgctggt tgtccaggca aagccgtctt ggtctgtcaa agcttcgcag
     3721 accacaccta atcgcataaa acgtaatttc atttcacggg cgacgaggcc agaactccct
     3781 gttccaaaga agtagatacg ctcagcatct tcgattagct gggcaattcg ctctagttgg
     3841 atttcgtcaa tcaagtcttg tgtttgttcc ctcatattgc tataacttct gaggactcgt
     3901 ttggtcagtg gactgtgctt ggagacttgg ttggcttgat tttctgcctg atgttggtat
     3961 tggaaaataa attctcggta gccagtaaag ccacactttt tagcaaagcg ggtcaaagca
     4021 gcttgagaaa tatgtaattt ttgggtgact tgttgagaag ataaatcatc tgtaatcgtt
     4081 tcagcttgca aaaaatagcg agcgatttct tgctctaggt ctgtcatttc ttcaaaatgt
     4141 gaatcaatga tagttgcgat atctggtttg tccataggga aagctccttt acatgagtca
     4201 tactggaaga ctagatcagg gaatagtcac acttcattat aacacataat ataaggatag
     4261 ataaataaaa acgcatctct gttttaaaaa cgaaaaaatc gaaaaagctt ctctcttttc
     4321 cataattttc tactcaaatt gtggtacaat taagagtaag attttaagtt agaaatgaga
     4381 ctgatttgta tgagaaaatt taacagccat tcgattccga ttcggcttaa tttattgttt
     4441 tcaatcgtca ttttactctt tatgaccatt attggtcgtt tgttgtatat gcaggttttg
     4501 aacaaggatt tttacgaaaa aaagctagcc tcagctagtc agaccaagat tacaagcagt
     4561 tcagcccgtg gggaaattta tgatgctagt ggaaaacctt tggtagaaaa tacgttaaag
     4621 caggttgttt cctttacgcg tagcaataaa atgacggcta cagacttaaa agaaacagct
     4681 aaaaagttac tgacttatgt gagcatcagt tctccaaatt tgacagaacg ccagctggcg
     4741 gattactatt tggctgatcc tgaaatctat aaaaaaatag tggaagctct cccaagtgag
     4801 aaacgcttgg attcagatgg caatcgtcta tccgaatcag aactgtataa caatgcggtc
     4861 gatagtgtac aaacgagtca actaaactat acagaggatg aaaagaaaga aatctatctt
     4921 tttagtcagt taaatgctgt tggaaacttt gcgacaggaa ccattgcgac agatcctcta
     4981 aatgattctc aggtggctgt tattgcctct atttcaaagg agatgcctgg cattagtatt
     5041 tctacttctt gggatagaaa ggttttggaa acttcccttt cttctatagt tgggagtgta
     5101 tccagtgaaa aagctggtct cccagcggaa gaagcagaag cctatcttaa aaaaggctat
     5161 tctctaaatg atcgtgtagg aacctcctat ttggaaaagc aatatgaaga gaccttacaa
     5221 ggaaaacgct cggtaaaaga aatccatctg gataaatatg gcaatatgga aagcgtggat
     5281 acaattgagg aaggtagtaa gggaaacaat atcaaactga ccattgattt ggccttccaa
     5341 gatagcgtgg atgctttact gaaaagttat ttcaattccg agctagaaaa tggtggagcc
     5401 aagtattctg aaggtgtcta tgcagtcgcc cttaacccaa aaacaggtgc ggttttgtct
     5461 atgtcaggga ttaaacatga cttgaaaacg ggagagttga cgcctgattc cttgggaacg
     5521 gtaaccaatg tctttgttcc aggttcggtt gtcaaggcgg cgaccatcag ctcaggttgg
     5581 gaaaatggag tcttgtcagg aaaccagacc ttgacagacc agtccattgt cttccaaggt
     5641 tcagctccca tcaattcttg gtatactcag gcttacggtt cattccctat cacagcggtc
     5701 caagctctgg agtattcatc aaatacctat atggtccaaa cagccttagg tcttatgggg
     5761 caaacctatc aacccaatat gtttgtcggc accagcaatc tagagtctgc tatggagaaa
     5821 ctgcgttcaa cctttggcga atatggcttg ggtactgcga caggaattga cctaccagat
     5881 gaatctactg gatttgttcc caaagagtat agctttgcta attacattac taatgccttt
     5941 gggcagtttg ataactatac gccgatgcag ttggctcagt atgtagcaac tattgcaaat
     6001 aatggtgttc gtgtggctcc tcgtattgtt gaaggcattt atggtaataa tgataaggga
     6061 ggactgggtg acttgattca gcaactgcaa ccgacagaga tgaataaggt caatatatcc
     6121 gactccgata tgagcatctt gcaccaaggt ttttatcagg ttgcccatgg tactagtgaa
     6181 ttgacaactg gacgtgcctt ttcaaatggc gccttggtat ccattagcgg aaaaacaggt
     6241 acagccgaaa gctatgtggc agatggtcag caagcaacca ataccaatgc ggtggcctat
     6301 gccccatctg ataatcccca aatcgctgtt gcagtggtct ttcctcataa taccaatcta
     6361 acaaatggtg taggaccttc cattgcgcgt gacattatca atctgtatca aaaataccat
     6421 ccaatgaact agaaaggaaa ttatgcttta tccaacacct attgccaagt tgattgacag
     6481 ttattctaag ttaccaggta tcgggattaa gacggctacg cgtctggcct tttatacgat
     6541 tgggatgtct gctgatgatg tcaatgaatt tgcaaaaaat ctcctttctg ctaagagaga
     6601 attgacatat tgttctattt gtggacgttt gacagacgac gatccttgtt ctatctgtac
     6661 tgatccgact cgtgaccaga caacaatttt agttcttgag gatagtagag atgtggcagc
     6721 catggaaaat atccaagaat accatggact ctatcatgtc cttcatggcc tcatttctcc
     6781 tatgaatggt atcagtccgg acgatatcaa tctcaagagc cttatgactc gtcttatgga
     6841 tagtgaggtt tcagaagtga ttgtggcgac taatgctaca gcggatggtg aagcgacttc
     6901 catgtatctt tcacgtttgc tcaagccggc tggtatcaag gttacgcgtc tagcacgagg
     6961 tctcgctgtg ggagcggaca ttgagtatgc ggacgaagtg acactcttac gagcgattga
     7021 aaatcggaca gagttgtaag tgtaggcaaa tttacgaact ccattcattt ataaaaaatc
     7081 aaagaggctg aaaatcgttc ctatcggcct ctttttgtat agtgtgatga gtaggatcag
     7141 gttcaagttt taaaaaacca agcaaatatg atatactaaa gagcgagtat tctagtagaa
     7201 ttaggacaaa taatatgaaa caaacgatta ttcttttata tggtggacgg agtgcggaac
     7261 gcgaagtctc tgtcctttca gctgagagtg tcatgcgtgc ggtcgattac gaccgtttca
     7321 cagtcaagac tttctttatc agtcagtcag gtgactttat caaaacacag gaatttagtc
     7381 atgctccggg gcaagaagac cgtctcatga ccaatgaaac cattgattgg gataagaaag
     7441 ttgcaccaag tgctatctac gaagaaggtg cagtggtctt tccagtcctt cacgggccaa
     7501 tgggagaaga tggctctgtt caaggattct tggaagtttt gaaaatgcct tacgttggtt
     7561 gcaacatttt gtcatcaagt cttgccatgg ataaaatcac gactaagcgt gttctggaat
     7621 ctgctggtat tgcccaagtt ccttatgtgg ctatcgttga aggcgatgat gtgactgcta
     7681 aaatcgctga agtggaagaa aaattggctt atccagtctt cactaagccg tcaaacatgg
     7741 ggtctagtgt cggtatttct aagtctgaaa accaagaaga actccgtcaa gccttaaaac
     7801 ttgccttccg atatgacagc cgtgtcttgg ttgagcaagg agtgaatgcc cgtgaaattg
     7861 aggttggcct cttgggtaac tacgatgtca agagcacgct acctggagaa gttgtcaagg
     7921 acgttgcctt ttatgactac gatgccaagt atattgataa caagattact atggatattc
     7981 ctgccaaaat cagtgatgat gtggtggctg tcatgcgtca aaatgcagaa acagccttcc
     8041 gtgccattgg tggccttggt ctatctcgtt gcgatttctt ctatacagat aagggagaga
     8101 tttttctcaa cgagctcaat accatgccag gttttaccca gtggtctatg tacccactac
     8161 tttgggacaa tatggggatc agctacccag aactaatcga acgtttggtt gaccttgcca
     8221 aggaaagctt tgacaagcgc gaagcgcatt tgatataaaa tgaaagagag ggtagaagcc
     8281 agaaccatca ctgcacggtg actagagttc tcggacttca gcccttttta aaggagtaga
     8341 aatgaaatta acaatccatg aaattgccca agttgttgga gccaaaaatg atatcagtat
     8401 ctttgaggac acccagttag aaaaagctga gtttgatagt cgtttgattg gaactggaga
     8461 tttatttgtg ccacttaaag gtgcgcgtga tggccatgac tttattgaaa cagcctttga
     8521 aaatggtgca gcagtaacct tgtctgagaa agaggtctca aatcatcctt acattttagt
     8581 agatgatgtt ttgacagcct ttcaatccct agcatcctac tatcttgaaa aaacgactgt
     8641 tgatgtcttt gctgttacag gttcaaatgg caagacaacg actaaggata tgttggcgca
     8701 tttactgtca acaagataca agacctacaa aacacaaggc aattacaata atgagattgg
     8761 ccttccttac acagttctcc acatgcctga aggaacagaa aagttggtct tggagatggg
     8821 gcaggaccac ttgggagata ttcatctctt gtctgaattg gctcgtccaa aaacagccat
     8881 cgtgaccttg gttggagaag ctcatttggc ctttttcaaa gatcgttctg agattgctaa
     8941 aggaaaaatg caaattgcag acggaatggc ttcaggttcc ttgtttttag cgccggctga
     9001 ccctatcgta gaggactatt tgccaactga taaaaaggtg gttcgttttg gtcaaggagc
     9061 agagctggaa attactgact tggttgagcg caaagatagt ctgaccttca aggccaattt
     9121 cttagagcaa gtccttgatt tgccagtaac tggcaagtac aatgcgacaa atgctatgat
     9181 tgcatcctat gttgccttgc aagaaggagt ttcagaggag caaattcgtt tggccttcca
     9241 acatcttgaa ttgacgcgta accgtaccga gtggaagaaa gcagtcaatg gagcagatat
     9301 cctgtcagat gtttacaatg ccaatccaac tgctatgaaa ctgattttag agactttctc
     9361 tgccattcca gccaatgaag gtggcaagaa aattgcagtt ttggcggata tgaaggagct
     9421 tggtgaccag tctgttcaac ttcataatca gatgattttg agcctctccc cagatgtgct
     9481 tgatatcgtt attttctatg gagaagatat tgctcagttg gctcaattgg ccagtcaaat
     9541 gtttccaatc ggccacgttt actacttcaa gaaaacagaa gaccaggatc aatttgaaga
     9601 cctagtcaag caggttaagg aaagccttgg agcctatgac caaatcttgc taaaaggttc
     9661 taactctatg aatctagcca aattggtaga aagtttagaa aatgaagcca agtgattttg
     9721 ttaagtatct gcaaagaatg attgccctta cagatactgg cttaaccttt acaaaagatc
     9781 cttttgaccg tgagcgctac gaggacttgc gaagtctgtt atctgaaatg ttgaatcagg
     9841 gatcagacct ggatgtagaa gaagtagcag aagtcttgaa accaacttca gcttatgcga
     9901 ctcccttaat ggacgtccgt gcttggattg ttgaggatga gaagatttgt ctggttaggg
     9961 gacaaggaga ggatagttgg gctttgccag gtggctttgg tgaagtcggc tattctccaa
    10021 ctgaaaatat tctcaaggaa attgaagaag aaaccggttt taaagccaaa gttgaaagac
    10081 tgctagctgt ttttgatacc aatcgtttcc aactacagag caaacaatat gcaaagtttg
    10141 tttttgaatg taagcttctc gatggacaat tccaagaaaa tcaagaaatc gctgaccttc
    10201 aattttttgc aattgaccaa ctgccgaatt tatctgaaaa acgcattacc aaggagcaaa
    10261 tcgagattct ttggcaggtt tatcaaggtc agagggagca atatcttgac taagaagatg
    10321 attatcgtat ttctaaattc atttttctta agtagcatgg tataataaaa tgcaggaaaa
    10381 ttttgaatca tgaggaagac tagatgaatt tatgggatat tttctttacg actcaggcaa
    10441 ccgagccgcc caaatttgac cttttttggt atgttagcct atttacgctc ttagccttaa
    10501 ccttttatac agcccatcgc tatcgtgaaa agaaggttta ccaacgattt ttccaaatct
    10561 tgcagactgt tcagttaatc cttctttatg gttggtactg ggtcaatcat atgccactgt
    10621 cagaaagcct acccttttac cattgccgta tggctatgtt tgtggtactc ttgcttcctg
    10681 gtcagtccaa atataaacaa tactttgcat tattgggaac atttgggaca ttagcagcct
    10741 ttgtttatcc agtgccagat gcttaccctt ttccacatat caccattcta tcctttatct
    10801 ttggtcattt agcactcttg gggaactctc tagtttatct attgagacag tataatgcgc
    10861 gattgctgga tgtgaaggga atttttctca tgacctttgc cctaaatgcc ttgatttttg
    10921 tggtcaattt ggtgacaggt ggcgattacg gatttttgac aaaaccgcca ttggttgggg
    10981 atcacggtct agtagctaat tatttacttg tttcaattgt gctggtagct actatcagtt
    11041 tgactaagaa aatcttagaa ttctttttag ctcaagaagc agaaaaaatg attgcaaagg
    11101 aagcttaaca cagagctttc ttttttgctc ttagagagtt tttacaagca gcttataaaa
    11161 taagaatttc tgaatagaca aactcaaaaa atggctggga aatttaggaa aaaagcaagc
    11221 acgattaaat tttttgtgtt ataatatttt gtgaatagct atgcctatgt ttagctatgg
    11281 aataatacga agtgcgaaac ttggaagata gagaggaagc gatgtaatgg ctagagaagg
    11341 cttttttaca ggtctagata ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag
    11401 aaatggtgaa ttaaatgtaa ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg
    11461 aattattgtt gatattgatg cagcagcaac tgctatcaag tcagccattt cccaagcgga
    11521 agaaaaggca ggcatttcga ttaaatcagt aaatgtcggc ttgcctggta atcttttgca
    11581 ggtagaacca actcagggga tgattccagt aacatctgat actaaggaaa ttacggatca
    11641 agatgttgaa aatgttgtca aatcagcttt gacaaagagt atgacacctg accgtgaagt
    11701 cattaccttt attcctgaag aatttattgt ggatggtttc caaggaattc gtgacccacg
    11761 tggcatgatg ggggttcgcc ttgaaatgcg tggtttgctt tatacaggac ctcgtactat
    11821 cttgcacaat ttgcgtaaga cggttgagcg tgcaggtgtt caggttgaaa atgttatcat
    11881 ttcaccacta gcaatggttc aatctgtttt gaacgaaggg gaacgtgaat ttggtgctac
    11941 agtgattgat atgggggcag gtcaaacgac tgtcgctaca atccgtaatc aagaactcca
    12001 gttcacacat attctccaag aaggtggaga ttatgtaact aaagatatct ccaaggtttt
    12061 gaaaacctct cgcaaattag cggaaggctt gaaactgaat tacggggaag cctatccgcc
    12121 tcttgcaagc aaagaaacct tccaagtaga ggttattgga gaagtagaag cagtcgaagt
    12181 gacggaagcc tacttgtcag aaattatttc tgcacgaatc aagcacatcc ttgaacaaat
    12241 caagcaagaa ttagatagaa ggcgtctatt ggacctccct ggtggtattg tcttaatcgg
    12301 tgggaatgcc attttaccag gtatggttga gcttgctcag gaagtctttg gcgtccgtgt
    12361 caagctttat gttccaaatc aagttggtat ccgtaatcca gcctttgcgc atgtgattag
    12421 tttatcagaa tttgcgggtc aattaacaga agttaatctt ttggctcagg gagcgataaa
    12481 aggtgagaat gacttaagtc atcagccaat tagttttggt gggatgctgc aaaaaacagc
    12541 tcagtttgta caatcaacgc ctgttcaacc agctcctgct ccagaagtag agccggtggc
    12601 gcctacagaa ccaatggcgg atttccaaca agcttcacaa aataaaccga aattagcaga
    12661 tcgtttccgt ggcttgatcg gaagcatgtt tgacgaataa agaggaaaaa taaattatga
    12721 cattttcatt tgatacagct gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg
    12781 gaggtggtgg caatgccatc aaccgtatgg tcgacgaagg tgttacaggc gtagaattta
    12841 tcgcagcaaa cacagatgta caagcattga gtagtacaaa agctgagact gttattcagt
    12901 tgggacctaa attgactcgt ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag
    12961 ccgctgaaga aagcgaagaa acactgacgg aagctattag tggtgccgat atggtcttca
    13021 tcactgctgg tatgggagga ggctctggaa ctggagctgc tcctgttatt gctcgtatcg
    13081 ccaaagattt aggtgcgctt acagttggtg ttgtaacacg tccctttggt tttgaaggaa
    13141 gtaagcgtgg acaatttgct gtagaaggaa tcaatcaact tcgtgagcat gtagacactc
    13201 tattgattat ctcaaacaac aatttgcttg aaattgttga taagaaaaca ccgcttttgg
    13261 aggctcttag cgaagcggat aacgttcttc gtcaaggtgt tcaagggatt accgatttga
    13321 ttaccaatcc aggattgatt aaccttgact ttgccgatgt gaaaacggta atggcaaaca
    13381 aagggaatgc tcttatgggt attggtatcg gtagtggaga agaacgtgtg gtagaagcgg
    13441 cacgtaaggc aatctattca ccacttcttg aaacaactat tgacggtgct gaggatgtta
    13501 tcgtcaacgt tactggtggt cttgacttaa ccttgattga ggcagaagag gcttcacaaa
    13561 ttgtgaacca ggcagcaggt caaggagtga acatctggct cggtacttca attgatgaaa
    13621 gtatgcgtga tgaaattcgt gtaacagttg tcgcaacggg tgttcgtcaa gaccgcgtag
    13681 aaaaggttgt gactccacaa gctagatctg ctactaacta ccgtgagaca gtgaaaccag
    13741 ctcattcaca tggctttgat cgtcattttg atatggcaga aacagctgaa ttgccaaaac
    13801 aaaatccacg tcgtttggaa ccaactcagg catctgcttt tggtgattgg gatcttcgcc
    13861 gtgaatcgat tgttcgtaca acagattcag tcgtttctcc agtcgagcgc tttgaagccc
    13921 caatttcaca agatgaagat gaattggata cacctccatt tttcaaaaat cgttaagtaa
    13981 atgaatgtaa aagaaaatac agaacttgtt tttcgagaag ttgcagaggc tagtctgagt
    14041 gctaatcgag agtgtggttc ggtctctgtc attgcagtta ccaagtatgt agatgtaccg
    14101 acagcggaag ccttgcttcc gctaggtgtt catcatatcg gtgaaaatcg tgtagataag
    14161 tttctggaaa aatatgaagc tttaaaagat cgagatgtga cttggcattt gattggtacc
    14221 ttgcaaagac gtaaggtgaa agatgtcatt caatacgttg attatttcca tgcattggac
    14281 tcagtaaagc tagcagggga aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt
    14341 caagtaaata tttctaaaga agaaagcaaa cacggttttt cgagagagga actgctggaa
    14401 atcttgccag agttagccag actagataag attgaatatg ttggtttaat gacgatggca
    14461 ccttttgagg ctagcagtga gcagttgaaa gagattttca aggcgaccca agatttacaa
    14521 agagaaattc aagagaaaca aattccaaat atgcctatga ccgagttaag tatgggaatg
    14581 agtcgtgatt ataaagaagc gattcaattc ggttccactt ttgttcgtat aggtacatca
    14641 ttttttaagt aggagagaac catgtcttta aaagatagat tcgatagatt tatagattat
    14701 tttacggagg atgaggattc aagtctccct tatgaaaaaa gagatgagcc tgtgtttact
    14761 ccagtaaatt cttcacagga accggctctc ccaatgaatc aaccttcaca gtcggctggt
    14821 acaaaagaga acaatatcac cagacttcat gcaagacaac aggaattggc aaatcagagt
    14881 cagcgtgcaa cggataaggt cattatagat gttcgttatc ctagaaaata tgaggatgca
    14941 acagaaattg ttgatttttt ggcaggaaac gaaagtatct tgattgattt tcagtatatg
    15001 acagaggtgc aggctcgtcg ttgtttggac tatttggatg gagcttgtca tgttttagct
    15061 ggaaatttga aaaaggtagc ttctaccatg tatttgttga caccagtgaa tgttattgta
    15121 aatgttgaag atatccgttt accagatgaa gatcaacagg gtgagttcgg ttttgatatg
    15181 aagcgaaata gagtacgata atgatttttt taattcgtat gatttataat gcagtggata
    15241 tttactccct gattttggta gccttcgctg tcatgtcttg gtttccaggt gcctacgaat
    15301 ccagtttagg tcgttggatt gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc
    15361 tgcctttaca gatagcgggt cttgatttat ctgtttgggt cgcgattgtt ttggttcgat
    15421 ttttaggaga aaacctagtg cgttttctgg cgatgatagg atgaataaag ggatttatca
    15481 gcatttctcc atagaagatc gtccatttct tgacaaggga atggaatgga taaagaaggt
    15541 agaagatagc tatgctcctt ttttaactcc ttttatcaat cctcatcagg agaagctatt
    15601 aaagattttg gccaaaacct atggtcttgc ttgtagcagt agtggggaat tcgtctcgag
    15661 tgagtatgtt cgagttttat tatacccaga ttatttccaa ccagagtttt cagattttga
    15721 aatatctctc caggaaattg tgtattccaa taaatttgaa catttaacgc atgctaagat
    15781 tttagggaca gtcatcaatc aattagggat tgaacggaaa ctttttggag atatcctagt
    15841 agatgaagaa cgggcgcaga ttatgattaa tcagcagttt cttcttctct ttcaagatgg
    15901 actaaagaaa attggtcgta tacctgtttc gctggaggaa cgtcctttca ccgagaaaat
    15961 agataagcta gaacagtatc gagaactgga tttatctgtg tctagttttc gattagatgt
    16021 tcttttatca aatgttttga aactatctag gaatcaagca aaccagttga ttgaaaagaa
    16081 acttgtccaa gtaaattatc atgtggtaga caaatcagat tacactgttc aagttggaga
    16141 cttgattagt gtgagaaaat ttggtcgctt gagattactt caagataagg gacaaacgaa
    16201 aaaagagaag aaaaaaataa ccgtccagtt attattaagt aagtgaggaa tagaatgcca
    16261 attacatcat tagaaataaa ggacaagact tttggaactc gattcagagg ttttgatcca
    16321 gaagaagtcg atgaattttt agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg
    16381 aatcatgata aaaatttgcg tattaagagt ttagaagagc gtttgtctta ctttgatgaa
    16441 ataaaagatt cattgagcca gtctgtattg attgctcagg atacagctga gagagtgaaa
    16501 caggcggcgc atgaacgttc aaacaatatc attcatcaag cagagcaaga tgcgcaacgc
    16561 ttgttggaag aagctaaata taaggcaaac gagattcttc gtcaagcaac tgataatgct
    16621 aagaaagtcg ctgttgaaac agaagaattg aagaacaaga gccgtgtctt ccaccaacgt
    16681 ctcaaatcta caattgagag tcagttggct attgttgaat cttcagattg ggaagatatt
    16741 ctccgtccaa cagctactta tcttcaaacc agtgatgaag cctttaaaga agtggttagc
    16801 gaagtacttg gagaaccgat tccagctcca attgaagaag aaccaattga tatgacacgt
    16861 cagttctctc aagcagaaat ggaagaatta caagctcgta ttgaggtagc cgataaagaa
    16921 ttgtctgaat ttgaagctca gattaaacag gaagtggaaa ctccaactcc tgtagtgagt
    16981 cctcaagttg aagaagagcc tctgctcatc cagttggccc aatgtatgaa gaaccagaag
    17041 tagctccagt gcatccgaca ggtccaacac cagctacaga aactgttgat tcagcaccag
    17101 gatttgaagc accgcaagaa tccgttacaa ttttataaga aacgatgtga gaacaatatc
    17161 ttatccttat atttccagcg agcaggagat ggtgtgagtc ctgcattccc taacgataag
    17221 attatcctct caaaaactca agtctgaatc tagtaagatt tgacgtttcc cacgttacgg
    17281 gataagaggg agaaagacta aatctttttc cgaataaagg tggtaccacg attttcgtcc
    17341 tttttggaag tcgtggtttt taatttgtta ttatttataa aggagatacc atgaaactaa
    17401 aagataccct taatcttggg aaaacagctt tcccaatgcg tgcaggcctt ccgaccaaag
    17461 agccagtttg gcaaaaggaa tgggaagatg caaaacttta tcaacgtcgt caagaattga
    17521 accaaggaaa acctcatttc accttgcatg atggccctcc atacgctaac ggaaatatcc
    17581 acgttggaca tgctatgaac aagatttcaa aagatatcat tgttcgttct aagtctatgt
    17641 caggatttta cgcaccattt attcctggtt gggatactca tggtctgcca atcgagcaag
    17701 tattgtcaaa acaaggtgtc aaacgtaaag aaatggactt ggttgagtac ttgaaacttt
    17761 gccgtgagta cgctctttct caagtagata aacaacgtga agattttaaa cgtttgggtg
    17821 tttctggtga ctgggaaaat ccatatgtga ccttgactcc tgactatgaa gcagctcaaa
    17881 ttcgtgtatt tggtgagatg gctaataagg gttatatcta ccgtggtgcc aagccagttt
    17941 actggtcatg gtcatctgag tcagcccttg ctgaagcaga gattgaatac catgacttgg
    18001 tttcaacttc cctttactat gccaacaagg taaaagatgg caaaggagtt ctagatacag
    18061 atacttatat cgttgtctgg acaacgactc catttaccat cacagcttct cgtggtttga
    18121 cggttggtgc agatattgat tacgttttag ttcaacctgc tggtgaagct cgtaagtttg
    18181 tcgttgctgc tgaattattg actagcttgt ctgagaaatt tggctgggct gatgttcaag
    18241 ttttggcaac ttaccgtggc caagaactca accacatcgt aacagaacac ccatgggata
    18301 cagctgtaga agagttggta attcttggtg accacgttac gactgactct ggtacaggta
    18361 ttgttcatac agcccctggt tttggtgagg acgattacaa tgttggtatt gctaataatc
    18421 ttgaagtcgc agtgactgtt gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat
    18481 ttgaaggtca attctatgaa aaggtagttc caactgttat tgaaaaactt ggtaacctcc
    18541 ttcttgccca agaagaaatc tctcactcat atccatttga ctggcgtact aagaaaccaa
    18601 tcatctggcg tgcagttcca caatggtttg cctcagtttc taaattccgt caagaaatct
    18661 tggatgctat tgataaagtt aaattccata cagaatgggg taaagtccgt ctttacaaca
    18721 tgattcgtga ccgtggtgac tgggtcatct ctcgtcaacg cgcttggggt gttccacttc
    18781 caatcttcta tgcagaagac ggtacagcta tcatgacagc tgaaactatt gaacacgtag
    18841 ctcaactttt tgaagaacat ggttcaagca tttggtggga acgtgatgcc aaagacctct
    18901 tgccagaagg atttactcat ccaggttcac caaacggcga gttcaaaaaa gaaactgata
    18961 tcatggacgt ttggtttgac tcaggttcat catggaatgg agtggtggta aaccgtcctg
    19021 aattgactta cccagccgac ctttacctag aaggttctga ccaataccgt ggttggttta
    19081 actcatcact tatcacatct gttgccaacc atggcgtagc accttacaaa caaatcttgt
    19141 cacaaggttt tgcccttgat ggtaaaggtg agaagatgtc taaatctctt ggaaatacca
    19201 ttgctccaag cgatgttgaa aaacaattcg gtgctgaaat cttgcgtctc tgggtaacaa
    19261 gtgttgactc aagcaatgac gtgcgtatct ctatgaatat tttgagccaa gtttctgaaa
    19321 cttaccgtaa gattcgtaac actcttcgtt tcttgattgc caatacatct gactttaacc
    19381 cagatcaaga tacagtcgct tacgatgagc ttcgttcagt tgataagtac atgacgattc
    19441 gctttaacca gcttgtcaag accattcgtg atgcctatgc agactttgaa ttcttgacga
    19501 tctacaaggc cttggtgaac tttatcaacg ttgacttgtc agccttctac cttgattttg
    19561 ccaaagatgt tgtttacatt gaaggtgcca aatcactgga acgccgtcaa atgcagactg
    19621 tcttctatga cattcttgtc aaaatcacca aactcttgac accaatcctt cctcacactg
    19681 cggaagaaat ctggtcatat cttgagtttg aaacagaaga cttcgtccaa ttgtcagaat
    19741 taccagaagt tcaaactttt gctaaccaag aagaaatctt ggatacatgg gcagccttca
    19801 tggactttcg tggacaagca caaaaagcct tggaagaagc tcgtaatgca aaagttatcg
    19861 gtaaatcact tgaagcacac ttgacagttt atccaaatga agtggtgaaa actctactcg
    19921 aagcagtaaa cagcaatgta gcacaacttt tgatcgtgtc tgagttgacc atcgcagaag
    19981 gaccagctcc ggaagctgcc cttagcttcg aagatgtagc cttcacagtt gaacgtgctg
    20041 ctggtgaagt atgtgaccgt tgccgtcgta ttgacccaac aacagcagaa cgcagctacc
    20101 aggcagttat ctgtgaccac tgtgtaagca tcgtagaaga aaactttgcg gaagcagtcg
    20161 cagaaggatt tgaagagaaa taagattgaa aagtctaggc aaaattcaat ttgagaagaa
    20221 aagacaacta attttatagt ctattaaacg cattgtatca cgtttttgaa cacctgatat
    20281 gatgcgtttt ttatttattt taaaaatttg cgaggtatga ctttttatac tcaacaagaa
    20341 tcaaagagaa acttagcaag ctaacagtag taagata
//
LOCUS       DAVMEF010000021        20013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMEF010000021 DAVMEF010000000
VERSION     DAVMEF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.021"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(47..967)
                     /gene="cysK"
                     /locus_tag="VYQ30_000542"
     CDS             complement(47..967)
                     /gene="cysK"
                     /locus_tag="VYQ30_000542"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1965704.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1252..1887)
                     /locus_tag="VYQ30_000543"
     CDS             complement(1252..1887)
                     /locus_tag="VYQ30_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1965705.1"
                     /translation="MEFRTIKEDDQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLRDVEVPVNLV"
     gene            1943..3241
                     /locus_tag="VYQ30_000544"
     CDS             1943..3241
                     /locus_tag="VYQ30_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1965706.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3238..3900
                     /locus_tag="VYQ30_000545"
     CDS             3238..3900
                     /locus_tag="VYQ30_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1965707.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            3980..4522
                     /gene="raiA"
                     /locus_tag="VYQ30_000546"
     CDS             3980..4522
                     /gene="raiA"
                     /locus_tag="VYQ30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1965708.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKV
                     ATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVEDQT
                     TNVIYRREDGEIGLLEVKES"
     gene            4658..6631
                     /locus_tag="VYQ30_000547"
     CDS             4658..6631
                     /locus_tag="VYQ30_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1965709.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6628..7080
                     /gene="rplI"
                     /locus_tag="VYQ30_000548"
     CDS             6628..7080
                     /gene="rplI"
                     /locus_tag="VYQ30_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1965710.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7124..8476
                     /gene="dnaB"
                     /locus_tag="VYQ30_000549"
     CDS             7124..8476
                     /gene="dnaB"
                     /locus_tag="VYQ30_000549"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1965711.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8478..8744
                     /locus_tag="VYQ30_000550"
     CDS             8478..8744
                     /locus_tag="VYQ30_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965712.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8833..10179
                     /gene="cbpD"
                     /locus_tag="VYQ30_000551"
     CDS             8833..10179
                     /gene="cbpD"
                     /locus_tag="VYQ30_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1965713.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10262..10364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10435..10725
                     /locus_tag="VYQ30_000552"
     CDS             10435..10725
                     /locus_tag="VYQ30_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1965714.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10688..11446
                     /locus_tag="VYQ30_000553"
     CDS             10688..11446
                     /locus_tag="VYQ30_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965715.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            11485..12492
                     /locus_tag="VYQ30_000554"
     CDS             11485..12492
                     /locus_tag="VYQ30_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1965716.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12492..13220
                     /locus_tag="VYQ30_000555"
     CDS             12492..13220
                     /locus_tag="VYQ30_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965717.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPQGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13339..13797
                     /locus_tag="VYQ30_000556"
     CDS             13339..13797
                     /locus_tag="VYQ30_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1965718.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            13799..16231
                     /locus_tag="VYQ30_000557"
     CDS             13799..16231
                     /locus_tag="VYQ30_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1965719.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQAHSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16873..17526
                     /gene="rr06"
                     /locus_tag="VYQ30_000558"
     CDS             16873..17526
                     /gene="rr06"
                     /locus_tag="VYQ30_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1965720.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17523..18854
                     /gene="hk06"
                     /locus_tag="VYQ30_000559"
     CDS             17523..18854
                     /gene="hk06"
                     /locus_tag="VYQ30_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1965721.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            18949..19476
                     /locus_tag="VYQ30_000560"
     CDS             18949..19476
                     /locus_tag="VYQ30_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1965722.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19555..>20013
                     /locus_tag="VYQ30_000561"
     CDS             19555..>20013
                     /locus_tag="VYQ30_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1965723.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTKTVALINELQNIKKTYLY
                     NLNVLKEKSELPSKIKAKLEVAFDQFKKDTLKPGEKVAEAEKKVAEAKKKA"
BASE COUNT         6195 a         3267 c         4549 g         6002 t
ORIGIN      
        1 agttctctag tctggagatt tttcaatata cttcgttatt ggatggttac aattcataaa
       61 gtgctgtaga gagataacgt tcaccgttat ctggtgctag ggcaaggact tttttacctg
      121 tacctaattt tttggcaacc tcgatggctc cgtagatagc tgcagctgag gaaatcccta
      181 caaggaagcc ttcttttcca ccaatttcac gtccgagtgc aagagcgtca tctgatgtta
      241 cacgaacgat accatcatag gctttagtat caagtgtatc aggaataaat ccagctgaga
      301 taccttgaat tttgtgagga ccaggttttt caccagatag aatagcagat tcatctgctt
      361 ctactgcaaa aacttgaatg ttagaatttt ctgatttgag tgcatgagaa acaccagaaa
      421 tcgttccacc ggtacctact ccagcaacaa aggcatctaa tccatcttta ccgaaagcag
      481 ctagtatctc agctcctgtt gttctttcgt gtacttctgg attagctgga ttgtcaaatt
      541 gaagaggaag gaaaccatca cgttcagcag cgatttcttg agccttagca atagcacctt
      601 tcattccctc gctaccagga gttaggacga gttcagcacc ataagcttgg ataattttac
      661 gtcgttctac actcatagtt tcaggcataa cgatgacgac tttataccct ttagcagcac
      721 ctacccatga aagtccaata ccagtgtttc cacttgttgc ttcaacaata gtagaaccag
      781 gtttcagaat accatcttgt tcagcttttt caatcatgct aagggcaata cggtctttta
      841 cagatgaacc aggattaaat gcttcaagct ttatatagac gtctgcagca ccttctggca
      901 cgatgttgtt aagtttaaca atcggtgttt gaccgattaa ttcagtaatg ttgttataaa
      961 tagacatatg aatacctctt tgtataagat atctttagta taacgatatc tataccattt
     1021 gtaaaatata gaaatcctat caaagcgata ggattttttt atatcataga tcaaagtcat
     1081 taattttcaa gtgattgtgt tcagctaatt ctagtaaata ggtgtgaagg aaaacgatat
     1141 caggtttcgt ttgaattgct tcattttcta gtatattttg gtacgcatct ttttattttg
     1201 ctcgctaaag tagttgtgag agtgtcaacg aaacgctgta ttattcattg tttacactaa
     1261 gtttacagga acttcaacat ctcgtaaacc ttggtcagtt aaagtgactt ttccattaaa
     1321 aaactccaca agtgcagctt taatagtttc tttttcttct ttatcaacat aaatcatcgt
     1381 atcgacttga tctgtaaagt ttgtatccag ctccatgaga tcatgttctt taaggaagtt
     1441 actgtactct tggtactgag cataagacat ttgaatagca atgccagcct gttcttttat
     1501 ttcaataata ccaatttctt tgacagctaa ggcgacactg ccggcgtaag cacgaattag
     1561 tcctccagcg cctagtttaa taccaccaaa gtagcgtgtc acgaccacac agacattggt
     1621 gagattgtga ttttctagta ccccaagcat gggaacacca gcagtaccac taggctcacc
     1681 atcatcactt gtacgtttaa tttcactacg ttctccaata ataaaggcag agcagttatg
     1741 tgtcgctttg tagtgttctt ttttgatggc agtaatgaag tcacgagcct cttcttcgct
     1801 ataaacacgc ttggcatggc agataaagcg agattttttg atttcttctt ggacttggtc
     1861 gtcctctttg attgttctaa attccatgat ttaattgtac taaaaaatca tctaaagtgc
     1921 tagtttttac gaataaagaa gtatgaaagt aaatttagat tatctcggtc gtttatttac
     1981 tgagaatgaa ttaacagaag aagaacgtca gttggcggag aaacttccag caatgagaaa
     2041 ggagaagggg aaacttttct gtcaacgttg taatagtact attctagaag aatggtattt
     2101 gcccatcggt gcttactatt gtcgagagtg cttgctgatg aagcgagtca gaagtgatca
     2161 aactttatac tattttccgc aggaggattt tccgaagcaa gatgttctca aatggcgcgg
     2221 ccaattaact ccttttcaag agaaggtgtc agagggactg cttcaagcag tagacaagca
     2281 aaagccaacc ttagttcatg cggtaacagg agctggaaag acagaaatga tttatcaagt
     2341 agtggctaaa gtaatcaatg cgggtggtgc agtgtgtttg gctagtcctc gcatagatgt
     2401 ttgtttggag ctgtataagc gcctgcaaca ggatttttct tgcgggatag ctttgctaca
     2461 tggagaatcg gaaccttatt ttcgaacacc actagttgtt gcaacaaccc atcagttatt
     2521 gaagttttat caagcttttg atttgctgat agtggatgaa gtagatgctt ttccttatgt
     2581 tgataatccc acgctttacc atgctgtcaa gaatagtgta aaggagaatg gattgagaat
     2641 ctttttaaca gcgacttcga ccaatgagtt agataaaaag gtccgtttag gagaactaaa
     2701 aagactgaat ttaccgagac ggtttcatgg aaatccgttg attattccaa aaccaatttg
     2761 gttatcggat tttaatcgct acttagacaa gaatcgtttg tcaccaaagt taaagtccta
     2821 tattgagaag cagagaaaga cagcttatcc gttactcatt tttgcttcag aaattaagaa
     2881 aggggagcag ttagaagaaa tcttacagga gcaatttcca aatgagaaaa ttggctttgt
     2941 atcttctgta acagaggatc gattagagca agtacaagct tttcgagatg gagaactgac
     3001 aatacttatt agtacgacaa tcttggagcg tggagttacc ttcccttgtg tggatgtttt
     3061 cgtagtagag gccaatcatc gtttgtttac caagtctagt ttgattcaga ttggtggacg
     3121 agttggacga agcatggata gaccgacagg agatttgctt ttcttccatg atgggttaaa
     3181 tgcttcaatc aagaaggcga ttaaggaaat tcagatgatg aataaggagg ctggtctatg
     3241 aagtgcttgt tatgtgggca gactatgaag actgttttaa cttttagtag tctcttactt
     3301 ctgaggaatg atgactcttg tctttgttca gactgtgatt ctacttttga aagaattggg
     3361 gaagagaact gtccaaattg tatgaaaaca gagttgtcaa caaagtgtca agattgtcaa
     3421 ttttggtgta aagaaggagt tggagtcagt catagagcga tttttactta caatcaagct
     3481 atgaaggatt ttttcagtcg gtataagttt gatggagact tcctgttaag aaaagttttc
     3541 gcttcatttt taagtgagga gttgaaaaag tacaaagagt atcaatttgt tgtaattccc
     3601 ctaagtcctg atagatatgc taatagagga tttaatcagg ttgagggctt ggtagaggca
     3661 gcaggctttg agtatctgga tttattagag aaaagagaag agagagccag ttcttctaaa
     3721 aatcgttcag agcgcttggg gacagaactt cctttcttta ttaaaagtgg agtcactatt
     3781 cctaaaaaaa tcctacttat agatgatatc tatactacag gagcaactat aaatcgtgtt
     3841 aagaaactgt tggaagaagc tggtgctaag gatgtaaaaa cattttccct tgtaagatga
     3901 ggaaaaaatt tgcaaaaata gaatgaaagc gttataataa aatcataaaa acaaaaatgt
     3961 ttagaaagaa ggtactcata tgattaaata tagtatccgt ggtgaaaacc tagaagtaac
     4021 agaagcaatt cgtgattatg tagtttctaa actcgaaaag atcgaaaagt acttccaacc
     4081 agaacaagag ttggatgccc gaattaactt aaaagtttat cgtgaaaaaa cggctaaagt
     4141 ggaagtaacg attccgcttg gatctattac tctccgcgca gaagatgtat ctcaagatat
     4201 gtatggttca attgaccttg taactgataa aattgaacgt cagattcgta aaaataaaac
     4261 aaaaatcgag cgtaaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga
     4321 agattcaaat gttgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat
     4381 ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt
     4441 ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt
     4501 gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa
     4561 ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt cgctttttga
     4621 ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc
     4681 aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt
     4741 tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt
     4801 tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt
     4861 aaaccaccaa gcggaagaaa gtttgacagc tctattggaa cagatgcctg taggtgttat
     4921 gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt
     4981 gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagt
     5041 aggaaatccg tctacttatg ccaagcttgg tgagaagcgt tatgctgttc atatggatgc
     5101 ttcttccggt gttttgtatt ttgtagatgt atccagggaa caagccataa cagatgaatt
     5161 ggtaacaagt cgaccagtga ttgggattgt ctctgtggat aattatgatg atttggagga
     5221 tgcaacttct gagtcagata ttagtcaaat caatagtttt gtagctaatt ttatatcaga
     5281 gttttcagaa aaatacatga tgttttctcg tcgggtaagt atggatcgat tttatctatt
     5341 tactgactac acggtgcttg agggcttgat gaatgataaa ttttctgtta ttgatgcttt
     5401 cagagaagag tcgaaacaga gacagttgcc cttgacctta agtatgggat tttcttatgg
     5461 cgatggaaat catgatgaga tagggaaagt tgctttgctc aatttgaact tggctgaagt
     5521 acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc cagtttattt
     5581 tggtggtggg tctgctgctt caatcaagcg tacacggact cgtacgcgcg ctatgatgac
     5641 agctatttca gataagattc ggagtgtaga tcaggttttt gtagtcggtc acaaaaattt
     5701 agacatggat gctttgggct ctgctgtagg tatgcagttg ttcgccagca atgtgattga
     5761 aaatagctat gctctttatg atgaagaaca aatgtctcca gatattgaac gagctgtttc
     5821 attcatagaa aaagaaggag ttacgaagtt gttgtctgtt aaggatgcaa tggggatggt
     5881 gaccaatcgt tctttgttga ttcttgtaga ccattcaaag acagccttaa cattatcaaa
     5941 agaattttat gatttattta cccaaaccat tgttattgac caccatagaa gggatcagga
     6001 ttttccagat aatgcagtca ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt
     6061 ggtaacggaa ttgattcagt tccagaattc taagaaaaat cgtttgagtc gtatgcaagc
     6121 aagtgtcttg atggctggta tgatgttgga tactaaaaat ttcacctcgc gagtaactag
     6181 tcggacattt gatgttgcta gctatctcag aacgcgcgga agtgatagta ttgctatcca
     6241 ggaaatcgct gcgacagatt ttgaagaata tcgtgaggtc aatgaactta ttttacaggg
     6301 gcgtaaatta ggttcagatg tactaatagc agaggctaag gacacgaaat gctatgatac
     6361 agttgttatt agtaaggcag cagatgccat gttagccatg tcaggtattg aagcgagttt
     6421 tgttcttgcg aagaatacac aaggatttat ctctatctca gctcgaagtc gtagtaaact
     6481 gaatgtacaa cggattatgg aagagttagg cggtggaggc cactttaatt tggcagcagc
     6541 tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa
     6601 tgaaataaag gaaaaggaga aagaagaatg aaagtaatct ttttagcaga tgttaaagga
     6661 aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa ctttcttatc
     6721 aaaaagaatc tagccaaaga agcgactgct caagctgtag gtgaacttcg tggtaaacaa
     6781 aaatcggaag aaaaagctca cgctgagatg attgcagaag gaaaagcaat taaagcacaa
     6841 cttgaagcag aagaaactgt tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc
     6901 tttggttcta ttaccaataa gaagattgca gaagaattgc aaaagcaatt tggaattaag
     6961 attgataaac gtcatattca agtacaagct ccgattcgag cggttggttt gattgatgtg
     7021 ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa
     7081 gtttacacct tcttgacaag attgtaaaag gaagggaagt ctgatggcag aagtagaaga
     7141 gttacgagta caacctcaag atatcttagc tgagcaatcc gttttagggg ctatctttat
     7201 tgatgagagt aaacttgttt ttgtgcgaga atacattgag tctcgggact tttttaagta
     7261 tgcccatcgt ttgattttcc aagccatggt cgatttatcc gatcgtggtg atgccataga
     7321 tgcaacaacg gttcgtacta tccttgataa tcaaggtgat ttacagaata ttggtggctt
     7381 gtcttacttg gttgagattg ttaattctgt gccaacttct gctaatgcgg agtattatgc
     7441 taagattgtt gcagaaaaag caatgctacg tcgtttaatt gccaagttga cagagtctgt
     7501 caaccaagct tacgaagcgt cacaaccagc tgatgaaatt attgctcagg cagaaaaagg
     7561 gttgattgat gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc gagatgtgtt
     7621 gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa acgaccgata ttacaggtat
     7681 tgcgacaggt tatcgtgatt tggatcatat gacaacagga cttcatgagg aggagttgat
     7741 tatcttagca gctcgtccag cagttggtaa gacagcattt gccttgaata tcgctcagaa
     7801 cattgggact aagttggaca aaacggttgc tattttttca ctcgaaatgg gtgcggaaag
     7861 cttggtagac cgtatgttag ctgcagaagg cttggtggag tcacattcta tccgtacagg
     7921 gcaattgaca gatgaggagt ggcaaaaata tactattgct cagggtaatc tagctaacgc
     7981 cagtatctat atcgatgata cgccaggtat tcggattaca gagattcgtt ctcgttctcg
     8041 taaattggct caagaaactg gaaatcttgg tttgattttg atagactatt tgcaacttat
     8101 cacgggaact ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc gtcagttgaa
     8161 aatactagcc aaggaattga aggttccagt aatcgctctg agtcagcttt ctcgtggtgt
     8221 agaacaacgt caggacaaga gaccggtctt gtctgatatt cgtgaatctg ggtctattga
     8281 gcaggacgct gatatcgtag cttttctcta tcgcgatgac tactatgaac gtggtggtga
     8341 agaagaggag ggtatcccaa ataataaggt ggaagttatt atcgagaaaa accgtagtgg
     8401 agctcgtgga acagtggaat tgattttcca aaaagaatac aataaatttt caagtatctc
     8461 aaagagggag gcataagatg tcagatgcat ttacagatgt agccaagatg aaaaaaatca
     8521 aagaagaaat caaggcacat gagggacaag tcgtagaaat gactttggag aatggtcgta
     8581 agcgccaaaa aaatagattg ggtaagctaa ttgaagttta tccatctcta tttattgtgg
     8641 agtttgggga tgtggaagga gataaacaag ttaatgttta cgttgaatcc tttacttact
     8701 cagatattct tacagaaaag aatttgattc attatcttga ctaaagtgag aaattttctc
     8761 actttttctt ttttctccga ataatttagg tgaaggcaat catcgcttta tattattttt
     8821 caaggaggaa gaatgaaaat tttaccgttt atagcaagag gaacaagtta ttacttgaag
     8881 atgtcagtta aaaagcttgt tcctttttta gtagtaggat tgatgctagc agctggtgat
     8941 agtgtctatg cctattccag aggaaatgga tcgattgcgc gtggggatga ttatcctgct
     9001 tattataaaa atgggagcca ggagattgat cagtggcgca tgtattctcg tcagtgtact
     9061 tcttttgtag cctttcgttt gagtaatgtc aatggttttg aaattccggc agcttatgga
     9121 aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg
     9181 acgattggtt ccattacttg gtctactgca ggaacttatg gtcatgttgc ctgggtgtca
     9241 aatgtaatgg gagatcagat tgagattgag gaatataact atggttatac agaatcctat
     9301 aataaacgaa ttataaaagc aaacacgatg acaggattta ttcattttaa agatttggat
     9361 ggtggcagtg ttgggaatag tcaatcctca acttcaacag gcggaactca ttattttaag
     9421 accaagtctg ctattaaaac tgaaccccta gttagtgcaa ctgtgattga ttactattat
     9481 cctggagaga aggttcatta tgatcagata cttgaaaaag acggctacaa gtggttgagt
     9541 tatactgcct ataatggaag ctatcgttat gttcaattgg aggctgtgaa taaaaatcct
     9601 ctaggtaatt ctgttctttc ttcaacaggt ggaactcatt attttaagac caagtctgct
     9661 atcaaaactg aacccctagt tagcggaact gtgattgatt actattatcc tggggagaag
     9721 gttcattatg atcagatact tgaaaaagac ggctacaagt ggttgagtta tactgcctat
     9781 aatggaagtc gtcgctatat acagctagag ggagtgactt cttcacaaaa ttatcagaat
     9841 caatcaggaa atatctctag ctatggatcc aataatagtt caactgtcgg ttggaagaaa
     9901 ataaatggta gttggtatca tttcaaatca aatggttcta aatcaacagg atggctgaaa
     9961 gacggttcta gctggtatta tttgaaatta tctggtgaaa tgcagacagg atggttaaag
    10021 gagaatggct cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag
    10081 gtctctggta agtggtatta ttcttactct tcaggcgcct tagctgttaa tacgacggtg
    10141 gatggctaca gagtaaacag tgatggagaa cgagtataga aaattggagt aggagaaatt
    10201 tcctgctctt ttctgtaagc agtttccttg acatttttct tattttgcgc tatcatatat
    10261 acatataata tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac
    10321 ctgatctggg taatgccagc ggagggaacg atacttagtc taattttgca ccttttccat
    10381 gtatggtaaa ggtttttctt ttttttaaaa ggaaaacgag aagaggaggt tcttatgaaa
    10441 gcaagcattg ccttgcaagt tttaccccta gcacagggga ttgatcggat agctgttatt
    10501 gatcaggtca ttgcttatct gcaaactcaa gaagtgacga tggtagtgac accatttgaa
    10561 acggtcttgg aaggggagtt tgatgagctt atgcgcattc taaaagaagc gctggaagtg
    10621 gcagggcagg aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg agagatttta
    10681 agtattgatg agaaacttga aaagtatact gagacgacac attagtctat tgggctttct
    10741 cggagtcttg tcaatctggc agttagcagg ttttcttaaa cttctcccca agtttatcct
    10801 gccgacacct cttgaaattc tccagccctt tgttcgtgac agagaatttc tctggcacca
    10861 tagctgggcg accttgagag tggctttact ggggctgatt ttgggagttt tgattgcctg
    10921 tcttatggct gtgctcatgg atagtttgac ttggctcaat gacctgattt accctatgat
    10981 ggtggtcatt cagaccattc cgaccattgc catagctcct atcctggtct tgtggctggg
    11041 ttatgggatt ttgcccaaga ttgtcttgat tatcttaacg acaacctttc ccatcatcgt
    11101 cagcattttg gacggtttta ggcattgcga caaggatatg ctgaccttgt ttagtctgat
    11161 gcgggccaag ccttggcaaa tcctgtggca ttttaaaatt ccagtcagcc tgccctactt
    11221 ttatgctggt ctgagggtca gtgtctccta cgcttttatc acaactgtgg tatctgagtg
    11281 gttgggaggt tttgaggggc ttggtgttta tatgattcag tccaagaaat tgtttcagta
    11341 tgataccatg ttcgctatta ttattctggt atcgattatc agccttttag gtatgaagtt
    11401 ggtcgatatt agtgaaaaat atgttattaa atggaaacgt tcgtagaatt agaatgtttc
    11461 tgaaaaagaa aagaggaaat caaaatgaag aaaacatgga aagtgttttt aacgcttgta
    11521 acagctcttg tagctgttgt gcttgtggcc tgtggtcaag gaactgcttc taaagacaac
    11581 aaagaggcag aacttaagaa ggttgacttt atcctagact ggacaccaaa taccaaccac
    11641 acagggcttt atgttgccaa ggaaaaaggt tatttcaaag aagctggagt ggatgttgat
    11701 ttgaaattgc caccagaaga aagttcttct gacttggtta tcaacggaaa ggcaccattt
    11761 gcagtgtatt tccaagacta catggctaag aaattggaaa aaggagcagg aatcactgcc
    11821 gttgcagcta ttgttgaaca caatacatca ggaatcatct ctcgtaaatc tgataatgta
    11881 agcagtccaa aagacttggt tggtaagaaa tatgggacat ggaatgaccc aactgaactt
    11941 gctatgttga aaaccttggt agaatctcaa ggtggagact ttgagaaggt tgaaaaagta
    12001 ccaaataacg actcaaactc aatcacaccg attgccaatg gcgtctttga tactgcttgg
    12061 atttactacg gttgggatgg tatccttgct aaatctcaag gtgtagatgc taacttcatg
    12121 tacttgaaag actatgtcaa ggagtttgac tactattcac cagttatcat cgcaaacaac
    12181 gactatctga aagataacaa agaagaagct cgcaaagtca tccaagccat caaaaaaggc
    12241 taccaatatg ccatggaaca tccagaagaa gctgcagata ttctcatcaa gaatgcacct
    12301 gaactcaagg aaaaacgtga ctttgtcatc gaatctcaaa aatacttgtc aaaagaatac
    12361 gccagcgaca aggaaaaatg gggtcaattt gacgcagctc gctggaatgc tttctacaaa
    12421 tgggataaag aaaatggtat ccttaaagaa gacttgacag acaaaggctt caccaacgaa
    12481 tttgtgaaat aatgacagaa attagactag agcacgtcag ttatgcctat ggtcaggaga
    12541 ggattttaga ggatatcaac ctacaggtga cttcaggcga agtggtttcc atcctaggcc
    12601 caagtggtgt tggaaagacc accctcttta atctaatcgc tgggatttta gaagttcagt
    12661 cagggagaat tgtccttgat ggtgaagaaa atccccaggg gcgcgtgagt tatatgttgc
    12721 aaaaggatct gctcttggag cacaagacgg tgcttggaaa tatcattctg cccctcttga
    12781 ttcaaaaggt ggataaggca gaagctattt cccgagcgga taaaattctt gcgaccttcc
    12841 agctgacagc tgtaagagac aagtatcctc atgaacttag cggtgggatg cgccagcgtg
    12901 tagccttact ccggacctac ctttttgggc acaagctctt tctcttagat gaggccttta
    12961 gcgccttgga tgagatgaca aagatggaac tccacgcttg gtaccttgag attcacaagc
    13021 agttgcagct aacaaccttg attattactc acagtatcga ggaggccctc aatctcagtg
    13081 accgcatcta tatcttgaaa aatcgccctg ggcagattgt ttcagaaatt aaactagatt
    13141 ggtctgaaga tgaggacaag gaagttcaaa agattgccta caaacgtcaa atcttggcag
    13201 aattaggctt agataagtag aaaaataggg agttggtgaa gattatcctt taccagcgcc
    13261 ctttttcttt taaaaatgag aaaatttcgg tataatagtc aaacaaggtc aaggtttaaa
    13321 gagagaggtg ggtttgttat gagatttaaa aatacatcgg atcatattga ggcctacatc
    13381 aaggcgattt tagatcaatc tggtatcgtg gagttgcaac ggagtcagtt ggcagatacc
    13441 tttcaggttg ttcctagtca gattaactac gtgatcaaga cacgctttac ggaaagtaga
    13501 ggctacttgg ttgaaagtaa gcgtggtggc ggaggctaca ttcgtatagg acggattgag
    13561 ttttctagtc atcatgaaat gctccgggag ctgctttact cgattggtga gcgagtcagt
    13621 caagaaattt atgaggatat tctccagctt ttggttgagc aggaattgat gaccaagcag
    13681 gagatgaatt tgctagaatc agtagctttg gatcgcgttt taggagaaga agctccagtt
    13741 gttcgagcaa acatgctacg tcagatcata caagaggtag atagaaaagg gaagtaagat
    13801 gaactattca aaagcattga atgaatgtat cgaaagtgcc tacatggttg ctggacattt
    13861 tggagctcgt tatctagagt cgtggcactt gttgattgcc atgtctaatc acagttatag
    13921 tgtagcaggg gcaactttaa atgattatcc gtatgagatg gaccgtttag aagaggtggc
    13981 tttggaactg actgaaacgg actatagcca ggatgaaacc tttacggaat tgccgttctc
    14041 ccgtcgtttg caggttcttt ttgatgaagc agagtatgta gcgtcagtgg tccatgctaa
    14101 ggtactaggg acagagcacg tcctctatgc gattttgcat gatggcaatg ccttggcgac
    14161 tcgtatcttg gagagggctg gtttttctta tgaagacaag aaagatcagg tcaagattgc
    14221 tgctcttcgt cgaaatttag aagaacgggc aggctggact cgtgaagatc tcaaggcttt
    14281 acgccaacgc catcgtacag tagctgacaa gcaaaattct atggccaata tgatgggcat
    14341 gccgcagact cctagtggtg gtcttgagga ctatacgcat gatttgacag agcaagcgca
    14401 ttctggcaag ttagaaccag tcatcggtcg ggacaaggaa atctcacgta tgattcaaat
    14461 cttgagccgt aagactaaga acaaccctgt cttggttggg gatgctggtg tcgggaaaac
    14521 agctctggcc cttggtcttg cccagcgtat tgctagtggt gacgtgcctg cggaaatggc
    14581 taagatgcgc gtgttagaac ttgatttgat gaatgtcgtt gcagggacac gcttccgtgg
    14641 tgactttgaa gaacgcatga ataatatcat caaggatatt gaagaagatg gccaagtcat
    14701 cctctttatc gatgaactcc acaccatcat gggttctggt agcgggattg attcgactct
    14761 ggatgcggcc aatatcttga aaccagcctt ggcgcgtgga actttgagaa cggttggtgc
    14821 caccactcag gaagaatatc aaaaacatat cgaaaaagat gcggcacttt ctcgtcgttt
    14881 cgctaaagtg acgattgaag aaccaagtgt ggcagatagt atgactattt tacaaggttt
    14941 gaaggcgact tatgagaaac atcaccgtgt acaaatcaca gatgaagcgg ttgaaacagc
    15001 ggttaagatg gctcatcgtt atttaaccag tcgtcacttg ccagactctg ctatcgacct
    15061 cttggacgaa gcagcagcaa cagtgcaaaa taaggcaaag catgtaaaag cagacgattc
    15121 agatttgagt ccagctgaca aggccctgat ggatggcaag tggaaacagg cagcccagct
    15181 aatcgcaaaa gaagaggaag tacctgtcta caaagacttg gtgacagagt ctgatatttt
    15241 gaccaccttg agtcgcttgt caggaatccc agttcaaaaa ctgactcaga ctgacgctaa
    15301 gaaatatcta aatcttgaag cagaactcca taaacgggtt atcggtcaag atcaagctgt
    15361 ttcaagtatt agtcgtgcca ttcgccgtaa tcagtcagga attcgcagtc acaagcgtcc
    15421 gatcggttcc tttatgttcc taggacccac aggtgtcggg aaaactgaat tagccaaggc
    15481 tctggcagaa gttctttttg acgacgaatc agcccttatc cgctttgata tgagtgagta
    15541 tatggagaaa tttgcagcta gtcgtctcaa cggagctcct ccaggctatg taggatatga
    15601 agaaggtggg gagttgacag agaaggttcg caataaaccc tattccgttc tcctctttga
    15661 tgaggtagag aaggcccacc cagatatctt taatgttctc ttgcaggttc tggatgacgg
    15721 tgtcttgaca gatagcaagg gacgcaaggt cgatttttca aataccatta tcattatgac
    15781 atcgaatcta ggtgcgactg cccttcgtga tgataagact gttggttttg gggctaagga
    15841 tattcgtttt gaccaggaaa atatggaaaa acgcatgttt gaagaactga aaaaagctta
    15901 tagaccggaa ttcatcaacc gtattgatga gaaggtggtc ttccatagcc tatctagtga
    15961 ccatatgcag gaagtggtga agattatggt caagccttta gtggcaagtt tggctgaaaa
    16021 aggcattgac ttgaaattac aagcttcagc tctgaaattg ttagcaaatc aaggatatga
    16081 cccagagatg ggagctcgcc cacttcgcag aaccctgcaa acagaagtgg aggacaagtt
    16141 ggcagaactt cttctcaagg gagatttagt ggcaggcagc acacttaaga ttggtgtcaa
    16201 agcaggccag ttaaaatttg atattgcata aaagaataaa agtatcagca tctgaccata
    16261 agtcacagtg gagtgaaact caatgaaaat caaagagcaa actaggcagc tagccgcagg
    16321 ttgctcaaaa cactggtttg aggttgtaga tagagctgac gtggtttgaa gagattttcg
    16381 aagagtatga aactaaaacc tatagcttct aaacgattcg tggttttcac cattcaacac
    16441 aaaattcata tgtttattac cctccgtcgt atttgtctta gagcgtgtgt agtagaaaaa
    16501 gagcagtctt atctgaaatt tttattcttt caaaagagac ctgtttcttt tttgcatgtc
    16561 aaatccgttc tagctggtat ttgaaaaatc aaactaatat tcaataaaaa tcaaagaaca
    16621 aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtag atagagctga
    16681 cgtagtttga agagattttc gaagagtata agctgcaaga tgaatgattt tcttgtattg
    16741 acgttgttgt tgacaaaaag tagcggataa atgaaatcca ttccattatc atagatgata
    16801 ggctggtagg aaattttcaa atagcataca ggaaatagat gtatggagtt ctggtagtag
    16861 aaagggagag agatgaacat tttagttgca gatgacgagg aaatgattag agaaggaatt
    16921 gcagcatttc tgacagaaga gggttatcat gtcattatgg ctaaggatgg acaagaggtc
    16981 ttggaaaaat ttcaagatct ccctatccat ctcatggtac tggatttaat gatgcctagg
    17041 aagagtggtt ttgaagtgtt aaaagaaatc aatcaaaagc acgatattcc tgtcatcgtc
    17101 ttgagtgctc tgggagatga aactactcag tcacaggtat ttgatctcta tgctgatgat
    17161 catgtgacaa aacctttttc tttggtactg cttgtcaagc gtattaaggc gcttatcaga
    17221 cgttactacg tcatagagga tctttggcga tatcaggatg taacagtgga ttttacctct
    17281 tacaaagcac attataaaaa tgaagaaatt gatctcaaac caaaggaatt actggtacta
    17341 aagtgtttga ttcagcataa aaatcaagtt ttaagtagag agcagatatt ggaagaaatt
    17401 tcaaaagatg tagctgattt accttgtgat agggtcgttg atgtctatat tcgtactctt
    17461 cgcaaaaaat tagctttaga ttgtatcgtg actgtgaaaa atgttgggta taagattagc
    17521 ttatgataaa aaatcctaaa ttattaacca agtctttttt aagaagtttt gcaattctag
    17581 gtggtgttgg tctagtcatt catatagcta tttatttgac ctttcctttt tattatattc
    17641 aactggaggg ggaaaagttt aatgagagcg caagagtgtt tacggagtat ttaaagacta
    17701 agacatctga tgaaattcca agcttactcc agtcttattc aaagtccttg accatatctg
    17761 ctcaccttaa aagagatatt gtagataagc ggctccctct tgtgcatgac ttggatatta
    17821 aagatggaaa gctatcaaat tatatcgtga tgttagatat gtctgttagt acagcagatg
    17881 gtaaacaggt aaccgtgcaa tttgttcacg gggtagatgt ctacaaagaa gcaaagaata
    17941 ttttgctttt gtatctccca tatacatttt tggttacaat tgctttttcc tttgtttttt
    18001 cttattttta tactaaacgc ttgctcaatc ctctttttta catttcagaa gtgactagta
    18061 aaatgcaaga tttggatgac aatattcgtt ttgatgaaag taggaaagat gaagttggtg
    18121 aagttggaaa acagattaat ggtatgtatg agcacttgtt gaaggttatt catgagttgg
    18181 aaagtcgtaa tgagcaaatt gtaaaattgc aaaatcaaaa ggtttccttt gtccgtggag
    18241 catcacatga gttgaaaacc cctttagcca gtcttagaat tatcctagag aatatgcagc
    18301 ataatattgg agattacaaa gatcatccaa aatatattgc aaagagtata aataagattg
    18361 accagatgag ccacttattg gaagaagtac tggagtcttc taaattccaa gagtggacag
    18421 agtgtcgtga gaccttgact gttaagccag ttttagtaga tattttatca cgttatcaag
    18481 aattagctca ttcaataggt gttacaattg aaaatcaatt gacagatgct accagggtcg
    18541 tcatgagtct tagggcattg gataaggttt tgacaaacct gattagtaat gcaattaaat
    18601 attcagataa aaatgggcgt gtaatcatat ccgagcaaga tggctatctc tctatcaaaa
    18661 atacatgtgc gcctctaagt gaccaagaac tagaacattt atttgatata ttctatcatt
    18721 ctcaaatcgt gacagataag gatgaaagtt ccggtttggg tctttacatt gtgagtaata
    18781 ttttagaaag ctatcaaatg gactatagtt ttctccctta tgaacacggt atggaattta
    18841 agattagctt gtagacagat tagtttttta ttaaagttca catagggtta acataagtgt
    18901 attattcttt atatagataa aaatatagat aaaagaaagg atactaatat ggtattagcg
    18961 attattttag taacattcct tattcgattg atttttttaa agcgttcgat agagaatgag
    19021 aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa acgattaacg
    19081 ttggctcata ttgtttttta tctctcttgc ctcgttgaag caatggtgca caagacaatt
    19141 tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct gatgttgatg
    19201 ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt caataatcac
    19261 aaatatgtag atcatatctt gtttaggaca gtaaaacatc ctaattactt tttaaatatt
    19321 cttcctgagt tgattggctt gaccttgttg agtcatgctt atgtgacttt tgttttagtt
    19381 tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa gctattacat
    19441 gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgat ttatatacag
    19501 ttcatattga agtgatatag taaggttaaa gaaaaaatat agaaggaaat aaacatgttt
    19561 gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta aatttagtat tggagtagct
    19621 agtgtagtag ttgctagctt gttcttagga ggagtagttc atgcagaaga ggttggtggt
    19681 aggaataccc ccacggttac atctagtggg caagatatat cgaagaagta tgctgatgaa
    19741 gtcgagtcgc atctaaaaaa aatattgagt gagatccaaa cacaattaga tagaaaacga
    19801 cataccaaaa ctgtagcgct aattaacgag ttgcaaaaca ttaagaaaac gtatttgtat
    19861 aatttaaatg ttttaaaaga gaagtctgag ttgccgtcaa aaataaaagc aaagttagaa
    19921 gtagcttttg atcagtttaa aaaagataca ttgaaaccag gagaaaaggt agcagaagct
    19981 gagaagaaag ttgcagaagc taagaaaaaa gcc
//
LOCUS       DAVMEF010000022        19071 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMEF010000022 DAVMEF010000000
VERSION     DAVMEF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.022"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            64..771
                     /locus_tag="VYQ30_000562"
     CDS             64..771
                     /locus_tag="VYQ30_000562"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1965724.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            828..4553
                     /locus_tag="VYQ30_000563"
     CDS             828..4553
                     /locus_tag="VYQ30_000563"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1965725.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQKFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSCKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFKKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            4646..6088
                     /locus_tag="VYQ30_000564"
     CDS             4646..6088
                     /locus_tag="VYQ30_000564"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1965726.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            6307..7329
                     /locus_tag="VYQ30_000565"
     CDS             6307..7329
                     /locus_tag="VYQ30_000565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1965727.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            7326..7871
                     /gene="purN"
                     /locus_tag="VYQ30_000566"
     CDS             7326..7871
                     /gene="purN"
                     /locus_tag="VYQ30_000566"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1965728.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            7955..8464
                     /locus_tag="VYQ30_000567"
     CDS             7955..8464
                     /locus_tag="VYQ30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1965729.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            8489..10036
                     /gene="purH"
                     /locus_tag="VYQ30_000568"
     CDS             8489..10036
                     /gene="purH"
                     /locus_tag="VYQ30_000568"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1965730.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFSDNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            10158..11420
                     /gene="purD"
                     /locus_tag="VYQ30_000569"
     CDS             10158..11420
                     /gene="purD"
                     /locus_tag="VYQ30_000569"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1965731.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     TKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            11823..12311
                     /gene="purE"
                     /locus_tag="VYQ30_000570"
     CDS             11823..12311
                     /gene="purE"
                     /locus_tag="VYQ30_000570"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1965732.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEKQGKIAEESSNE
                     LI"
     gene            12298..13389
                     /gene="purK"
                     /locus_tag="VYQ30_000571"
     CDS             12298..13389
                     /gene="purK"
                     /locus_tag="VYQ30_000571"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1965733.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVISSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            13399..13626
                     /locus_tag="VYQ30_000572"
     CDS             13399..13626
                     /locus_tag="VYQ30_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1965734.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            13689..14987
                     /gene="purB"
                     /locus_tag="VYQ30_000573"
     CDS             13689..14987
                     /gene="purB"
                     /locus_tag="VYQ30_000573"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1965735.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(15042..18980)
                     /gene="strH"
                     /locus_tag="VYQ30_000574"
     CDS             complement(15042..18980)
                     /gene="strH"
                     /locus_tag="VYQ30_000574"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1965736.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIEQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGSPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAELVVAGLMSTLAAYGLTKRKED"
BASE COUNT         5152 a         3864 c         4565 g         5490 t
ORIGIN      
        1 tcttgtgttt tttggttttt tgtggtaaaa tgtgctcaag taatacgaaa ggcgaacttt
       61 aaaatgtcaa aacaattgat ctattcggga aaagctaaag atatctatac aactgaggat
      121 gaaaatctta ttatttcaac ttacaaggac caggcgactg ctttcaacgg tgtcaagaag
      181 gagcagattg caggtaaggg agtcttgaat aatcagatct catcttttat ttttgagaaa
      241 ttaaatgcgg ctggtgtggc gactcacttt gtggagaaac tttcagacac ggaacaactc
      301 aataaaaagg ttaagattat tcctttggaa gtcgtgctcc gcaactatac tgctggttcc
      361 ttttcaaaac gttttggtgt ggatgaggga atcgccttgg agactccgat tgtcgaattt
      421 tactacaaaa atgatgattt ggatgatcca tttatcaatg atgagcatgt gaaattccta
      481 cagattgcgg gtgaccagca gattgcctac ttgaaggaag aaacgcgtcg tatcaatgaa
      541 ctattgaaag tctggtttgc tgagattggg cttaaattga ttgactttaa gctagagttc
      601 ggttttgaca aggatggcaa gattatcttg gcagacgaat tttcaccaga taactgccgc
      661 ttgtgggacg ctgatggcaa ccacatggat aaggatgttt tccgtagagg attgggagaa
      721 ctaaccgacg tttatgagat tgtttgggaa aagttgcagg gtttaaaata acaacctcaa
      781 ggctgtttgg gaatattgca agagctgaaa taaaggaata agaattgatg gataaacgta
      841 tttttgttga aaaaaaggct gattttcagg tcaagtcaga gagtttggtt agagagctcc
      901 agcacaacgt gggactgtca agcttgaaaa gtattcgtat tgtgcaagta tatgatgtat
      961 ttgacttggc tgaggacttg tttgcacctg cagagaagca cattttctct gagcagggaa
     1021 ccgaccatgt tttagatgaa gtatctgtgc aggcggatct tgctaactat gctttctttg
     1081 ccattgaaag tctgccaggg cagtttgacc agcgtgcagc ttcgtcacag gaagccttgc
     1141 ttttgttggg aagttcgagt gacgtgacag tcaacacagc ccaactttac ttggtgaata
     1201 aagatattga tgcgactgag ttggaagctg tcaaaaacta cctgctcaat ccagttgatt
     1261 ctcgtttcaa ggatatcacg acagggattg ccaagcagaa gttttcagag tcagacaaga
     1321 ccattccaaa attgactttc tttgaaagct atacagcaga agactttgct cgctacaagg
     1381 ccgagcaagg gatggccatg gaagtggatg atttgctctt tatccaagac tactttaagt
     1441 caatcgggcg cgtgccaact gagactgaac tcaaggtttt ggacacttac tggtctgacc
     1501 actgccgtca tacgactttt gaaacagagt tgaaacacat cgacttttca gcttctaaat
     1561 ttcaaaagca attgcagtca acctatgaca agtatattgc catgcgcgag gagttaggtc
     1621 ggtctgaaaa accacaaacc ttgatggata tggcgactat tttcggtcgt tatgagcgtg
     1681 ctaatggacg attggatgat atggaagtct ctgacgaaat caatgcctgc tcagttgaaa
     1741 ttgaagtgga tgttgatggt gtcaaggaac cttggctcct catgtttaaa aatgaaaccc
     1801 acaaccatcc aacagaaatt gagccatttg gtggagcggc tacctgtatc ggtggagcta
     1861 ttcgtgatcc gttgtcaggc cgttcctatg tttaccaagc catgcgtatt tcaggtgctg
     1921 gtgatattac agcaccgatt tcggaaactc gtgctgggaa attgccacaa caagtcattt
     1981 ctaaaacagc agctcatggt tattcttcat atggtaacca gattgggctt gccacaacct
     2041 acgttcgtga atacttccac ccaggctttg tagctaaacg tatggaactt ggtgccgttg
     2101 ttggtgcggc tcccaagggc aatgttgtcc gtgaaaaacc tgaagcaggt gatgtgatca
     2161 tccttctcgg aggcaaaaca ggtcgtgatg gtgtcggtgg tgcgacgggc tcttctaagg
     2221 ttcaaacagt tgagtctgta gagactgctg gtgctgaggt tcaaaaagga aatgccatcg
     2281 aagaacgcaa gattcagcgc ctcttccgta atggcaatgt cactcgtctg atcaagaagt
     2341 ccaatgactt tggggcaggc ggcgtctgtg tggctatcgg tgaattggca gacggtcttg
     2401 aaatcgacct caacaaggtg cctcttaaat accagggctt gaatggtaca gaaattgcca
     2461 tctctgaatc acaagaacgg atggcggtcg tggttcgtcc tgaagatgtg gatgccttcg
     2521 ttgccgaatg taacaaagaa aatattgatg ctgttgtggt ggcgacagta actgaaaaac
     2581 caaatcttgt catgcactgg aatggtgaga caatcgttga cttggagcgt cgtttccttg
     2641 acaccaatgg tgtgcgcgtg gttgtcgatg ccaaagttgt ggacaaggat gtcaaactcc
     2701 cagaagagcg tcaaacatct gctgaaacac tggaatcaga tacccttacg gttctatctg
     2761 acctcaacca tgcaagtcaa aaaggattac agactgtctt tgactgctct gttggacgct
     2821 caacggttaa tcacccactt ggtggtcgtt accaactcac accaactgag gcatctgtgc
     2881 agaaattgcc agttcaacac ggtgtgactc atactgcgtc ggtcattgct caaggtttca
     2941 acccatatgt agctgaatgg tctccatacc acggtgctgc ttacgcggtt atcgaagcaa
     3001 ctgctcgttt ggtggctgct ggtgccaact ggtctaaggc tcgtttctct taccaagaat
     3061 atttcgagcg gatggacaag caagcagagc gttttggtca gccagtagcc gcccttctag
     3121 gctctattga agctcagatt caacttggct tgccatctat cggtggtaag gactccatgt
     3181 ctggtacctt tgaagaattg accgttccgc caaccttggt tgcctttggg gtgacgacgg
     3241 cagatagctg taaggtgctc tctccagaat ttaaagctgt tggggaaaat atctactaca
     3301 tcccaggtca agccctctct gcagagattg attttgactt gattaagaaa aattttgctc
     3361 agtttgaagc aagccaagct gaccataaag tgacatctgc atcagctgtc aaatacggtg
     3421 gtgtagttga aagtttggct cttgctacct ttggaaacta tattggtgca gaggtgacct
     3481 tgcctgaact taaaacagct ttgacagctc aattaggcgg ctttgtcttc acatctcctg
     3541 aagaaattgc tggagtagag aagattggac aaacgaaagc agactttaca ctgactgtca
     3601 acggtgtgaa gctagatgga cacaagcttg acagtgcatt tcaagggaca ttggaagaag
     3661 tttacccaac agaatttacc caagcgaaag aactagaaga agtaccagct gtggcatcag
     3721 atgttgtgat taaagccaaa gaaaaggttg aaaaacctgt ggtttacatc ccagtctttc
     3781 caggaaccaa ctcagaatat gattcagcta aggccttcaa aaaagaaggt gcagaggtca
     3841 atttggtgcc attcgtgacc ttgaatgaag aagctattgt caagtcagtt gaaactatgg
     3901 ttgacaatat cgacaagact aatattctct tctttgccgg tggattctcg gctgcggatg
     3961 aaccagatgg ttcagctaag tttatcgtca atatcctgct taatgaaaaa gtgcgtgtgg
     4021 ctattgatag ctttatcgcc cgtggtggct tgattatcgg tatttgtaat ggattccaag
     4081 ccttagtcaa atcgggtctc ctaccatacg gaaactttga agatgctaac agtactagtc
     4141 caaccctctt ctacaatgat gccaaccaac acgtggccaa gatggtggaa actcgcattg
     4201 ccaataccaa ctcaccatgg ttggctggtg tgcaagtggg cgatatccac gctattcctg
     4261 tttcgcacgg tgaagggaag tttgtcgtga cggctgagga atttgcagag ctccgtgaca
     4321 atggacaaat tttcagccaa tacgttgact ttaacggtaa accaagtatg gattctaagt
     4381 acaatccgaa tggttctgtc catgccatcg aaggaattac cagcaagaat ggtcaaatca
     4441 tcggtaagat gggccactca gaacgttatg aggatggtct tttccaaaat atcccaggca
     4501 ataaagacca acacctgttc gcatcagcgg ttaaacattt cactggaaaa taagacttac
     4561 agattttcta atagatagta tcagtaatgt aaaagtcatg taaatctagc tcttgatgat
     4621 tacaaatgaa aattaggtat aaaaaatgac atacgaagta aaatctctta atgaagaatg
     4681 tggtgttttc ggtatttggg gacatccaga tgctgctaag ttgacctatt ttggactcca
     4741 cagtcttcaa caccgtggtc aggagggggc aggaatcctc tccaacgatc aaggacaact
     4801 gaagcgccat cgtgacatgg ggcttttatc agaagttttc agaaatccag ctaatttgga
     4861 taaattgaca ggagctggtg cgattgggca tgtgcgttat gcgactgctg gcgaagcttc
     4921 tgtagataac atccagccct tcctcttccg ttttcacgat atgcagtttg gcttggctca
     4981 taatggaaat ctgaccaatg cagcctctct caagaaagaa ctggaacaaa gaggagcaat
     5041 tttcagcgcg acttcggact ctgaaatctt ggctcacctc attcgtcgca gtcataatcc
     5101 tagcctgatg ggcaaaatca aggaagcgct cagccttgtc aaaggtggtt ttgcctatat
     5161 cttgctgttt gaggacaagt tgattgcggc tcttgaccct aatggctttc gtccgctttc
     5221 tatcgggaaa atggccaacg gagcggtggt tgtttcctct gaaacctgtg cttttgaggt
     5281 cattggtgcc gagtggattc gtgatttgaa gccaggtgag attgtgatca ttgatgacga
     5341 gggcattcag tatgacagct atacagatga tacccagttg gcgatttgtt ctatggagta
     5401 tatctacttt gctcgccctg attctaatat ccacggtgtc aatgtccata cggcacgtaa
     5461 gagaatggga gcgcaattgg cgcgagaatt taagcatgag gcagatattg tagttggtgt
     5521 gcccaattct tccctaagcg cggctatggg atttgcggaa gaatcaggct taccaaatga
     5581 aatgggtctg atcaaaaacc aatacaccca gcgaactttt atccaaccga ctcaagaatt
     5641 gcgggagcaa ggagtgcgga tgaaactgtc tgctgtttcg ggtgttgtca aaggcaaacg
     5701 tgtggtcatg gtggatgatt ccattgtacg tggaacaacc tctcgtcgta tcgttcagct
     5761 cttgaaagaa gcgggtgcga ctgaggttca cgttgccatt ggaagtcctg cactagcgta
     5821 tccatgtttc tacgggattg atatccagac ccgtcaggag ctgattgcag ccaatcatac
     5881 ggtcgaagaa actcgccaaa tcattggtgc ggacagtctg acttatcttt caattgatgg
     5941 cttgattgag tctattggta tcgaaacaga tgcgccgaac ggtggtctct gtgtcgctta
     6001 ctttgacggt gactacccaa cgcctctcta tgactacgaa gaagactatc gtagaagttt
     6061 ggaagaaaag accagttttt acaagtaggc gacagattct ccattaaaga aaaggaaaaa
     6121 acaaatgaca aataaaaatg catatgcctc acgtctcact actgactaaa ggcttaagca
     6181 tttagtcagt agacgctttg tcctatagga tcaaagctag agccctgact agtattttta
     6241 gataaaaaga tggtttatct aaaaatacgt cgcagtcttt ctcaaaaaaa gaaaaggaaa
     6301 aataaaatgg caaataaaaa tgcgtacgct caatctggtg tggatgttga agcgggttat
     6361 gaagttgttg aacggattaa aaagcacgtg gctcgtacgg agcgtgcagg tgtcatggga
     6421 gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa agaacccgtc
     6481 ttgatttcag ggactgacgg tgtcggaacc aagctcatgt tggctatcaa gtacgacaag
     6541 cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt
     6601 gcggaacccc tctattttct cgactacgta gcgacaggga agaatgaacc agctaagcta
     6661 gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt
     6721 ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt ggctggtttt
     6781 gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt agagggagat
     6841 gttcttctcg gacttgcttc aagtgggatt cactcaaatg gttactcttt ggttcgtcgt
     6901 gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag
     6961 gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccgct catcaaagaa
     7021 gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt
     7081 atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt gcttccaatt
     7141 ttcaaagccc ttgaaaaata tggtcagatt aaacacgaag aaatgtttga aatcttcaat
     7201 atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg
     7261 ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt
     7321 atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt caggtgattg
     7381 ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat gtgcttgagc
     7441 gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt gagagcaaga
     7501 cagactacga agcagccctt gtcgaactct tggaagaaca ccagattgac ttggtttgcc
     7561 tagcaggcta catgaaaatc gttggaccaa ccttattgtc ggcttatgaa ggtcggattg
     7621 tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt gaggatgctt
     7681 ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg ggtgtggata
     7741 caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc attgacagat
     7801 ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg aaggctctat
     7861 ttacagattg actttttgat gattcatatg atatctttga ttttaaattg gagtcagtgt
     7921 ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg
     7981 atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta agtttgaaac
     8041 aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga ttccattttc
     8101 agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atgttattta atctgatttc
     8161 ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta gtttaagaat
     8221 agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata ttttagcaat
     8281 aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtctgtg taggcttact
     8341 gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga tcaatatctt
     8401 aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt taattggtgt
     8461 ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca gcgtctcaga
     8521 caaagcgggc attgttgaat ttgcccaaga actcaaaaaa cttggttggg agattatctc
     8581 aacaggtgga actaaggttg cccttgataa tgctggggtg gataccattg ctatcgatga
     8641 tgtgactggt ttcccagaaa tgatggacgg tcgtgtgaag accctccacc caaatatcca
     8701 cggagggctt ctcgctcgtc gtgacttgga tagccacttg gaagcggcta aggacaacaa
     8761 gattgagctc attgaccttg tggtggtcaa cctttaccca tttaaggaaa ctatccttaa
     8821 accagatgtg acttatgctg atgcagttga aaatatcgat attggtgggc catctatgct
     8881 tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg gtagatcctg ctgactacgc
     8941 tgtggttttg gacgaattgg cagcaaacgg cgaaacctct tatgaaactc gccaacgttt
     9001 agcagccaaa gtatttcgtc acacagcggc ttatgacgcc ttgattgcag aatacttcac
     9061 agctcaagtg ggtgaaagca agcctgaaaa actcactttg acttatgacc tcaagcaacc
     9121 aatgcgttac ggtgagaatc ctcaacaaga cgcggacttt taccagaaag ctttgcctac
     9181 agattactct attgcttcag ccaaacagct caacgggaaa gaattgtcat ttaataatat
     9241 ccgtgatgca gatgctgcta tccgtatcat ccgtgacttc aaagatagtc caaccgttgt
     9301 ggctctcaaa cacatgaatc catgtggaat tggtcaagct gatgacatcg agactgcttg
     9361 ggactacgct tatgagtctg acccagtatc tatctttggt gggattgtcg tcctcaaccg
     9421 tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa tcatcattgc
     9481 cccaagctat acggatgaag cgctagctat tttgatcaat aaaaagaaaa acttgcgtat
     9541 ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat acacaggtgt
     9601 agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag ctgactggca
     9661 agtggtgacc aaacgtcagc caactgagac agaagcgact gctcttgagt tcgcttggaa
     9721 ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca tgacacttgg
     9781 tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg accaagccaa
     9841 agatcgtctg aacggggcgg tccttgcttc agatgccttc ttcccatttt cggataacgt
     9901 ggaagaaatc gccaaagcag gaattaaggc catcatccag cccggtggct ctgtccgtga
     9961 ccaagaatcc atcgaagcag cgaataaata cggcttgact atggtcttta caggtgtgag
    10021 acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt ttttggctta
    10081 aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat ataatgttgg taaataattc
    10141 gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc tggtggtcgt gagcatgcga
    10201 ttgctaaaaa gttacttgaa tcaaaagacg tggaaaaagt ctttgtagct cctgggaatg
    10261 atgggatgac tctggatggt ttggaattgg taaatatctc tatttccgaa cattctaaat
    10321 tgattgactt cgcaaagacc aatgatgttg cttggacctt tatcggtcca gatgatgccc
    10381 ttgctgctgg tatcgtggat gattttaacc aagctggact taaggccttt ggtccgacta
    10441 gggctgcagc ggagctggag tggtccaagg atttcgccaa ggaaatcatg gtcaaatacg
    10501 gcgttccgac agcaacatat ggcacatttt cagatttcga ggaagccaag gcctatatcg
    10561 aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt ggcacttggg aagggtgtcg
    10621 tcgttgctga gacggttgag caagcggtcg aagccgctca tgagatgctt ttggacaata
    10681 aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt ccttgaagga gaggaatttt
    10741 cactctttgc ctttgtcaat ggtgataagt tctacatcat gccaacggct caggaccaca
    10801 aacgtgccta tgatggcgac aaagggccta acacgggtgg tatgggtgcc tatgcgccag
    10861 tcccacactt accacagagt gtagttgata cagcggttga caccattgtc aagccagttc
    10921 tagaaggggt gattaaagaa ggtcgccctt atctgggagt tctttacgca gggcttatcc
    10981 tgacagctga tggaccgaaa gtcattgagt tcaacgctcg gttcggagat ccagaaactc
    11041 agattatctt gcctcgcttg acctctgact ttgctcaaaa tatcacagat atcctggata
    11101 gcaaggagcc aaatatcatg tggacggaca agggtgtgac tctgggtgtg gttgtcgcaa
    11161 ccaagggcta cccgctagac tatgaaaggg gcgttgagtt gccagccaag acagaaggcg
    11221 atgtcatcac ctactatgca ggggctaagt ttgcggaaaa tagcagagca ctgctctcaa
    11281 acggcggacg agtttatatg ctcgttacca cagcagatac cgtcaaagaa gcccaagcca
    11341 gcatatacca agaactatac caacaaaaaa tagaaggact cttctaccga acagatatcg
    11401 gaagcaaggc aattaagtaa agatataaga ataacgcgcc gtagtcgcca aacacgataa
    11461 tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg gtcaggggga aacttggaga
    11521 ccttaggctc aaagtttagg aatgaaaccg aaggtttgct tccgcctcca tcacctaaga
    11581 ccattatcaa aaagaaaaat aaaaattcac aaaatacgtt aatgatcgta tggtttgcga
    11641 gcgttagcga gctaatatag aacaatcacc gccgttgtga aagaacgatt ggatgataat
    11701 ccaatcgttc agggaaattg gaagaccttg ggtttccaat ttaggcatga gacacctttg
    11761 gtggctgctg ccgtccctca caagctaagg tgattgttga aaaagaggaa aaaggagaag
    11821 aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc cgactgggca accatgcaaa
    11881 aaacagcaga agtcctagac cgcttcggtg tagcctacga aaagaaagtt gtttccgcac
    11941 accgtacacc agacctcatg ttcaaacatg cagaagaagc ccgtagtcgt ggcatcaaga
    12001 tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg catggtagct gccaaaacaa
    12061 cccttccagt cattggtgtg ccagtcaagt ctcgtgctct tagtggagtg gattcacttt
    12121 attctatcgt tcagatgccg ggtggggtgc ctgttgcgac catggctatc ggtgaagctg
    12181 gagcgactaa cgcagctctc tttgccctcc gtctcctctc tgtagaagat aagtccattg
    12241 cggatgcact tgccaacttt gctgaaaaac aaggaaaaat cgcagaggag tcgtcaaatg
    12301 agctcatcta aaacaatcgg aattatcggt ggcggtcaac tgggtcagat gatggccatt
    12361 tctgctatct acatgggcca caaggttatc gcgctggatc ctgcggcgga ttgcccggcc
    12421 tctcgcgtgg cggaaatcat tgtggcacct tataacgatg tggacgccct ccgtcagttg
    12481 gcagaccgtt gcgatgtcct cacttacgag tttgaaaatg tcgacgctga cggtttggat
    12541 gccgttatca aggatggaca actccctcaa ggaacagatc tgctccgcat ttcgcaaaat
    12601 cgtatttttg aaaaggactt tttgtcaaac aaggctcaag tcactgtggc accctacaag
    12661 gtcgtgattt ctagcctaga cttggcagat atcgacttgt cgaaaaacta tgtcctcaag
    12721 actgcgactg gtggctacga tggtcatgga caaaaggtta ttcgttcaga agcagacttg
    12781 gaagcagcct atgcgctagc agactcagca gactgcgtct tggaagaatt tgtcaacttt
    12841 gaccttgaga tttctgtcat cgtgtcagga aatggcaagg aggtgacgtt tttcccagtt
    12901 caggaaaata tccaccgcaa caatatcctg tctaagacca tcgtaccagc ccgcatttct
    12961 gaaagtctag cagacaaggc taaagctatg gcagtgcgaa tcgcagaaca actcaacttg
    13021 tctggaactc tctgtgtgga aatgtttgcg actgatgatg acgttattgt caatgaaatc
    13081 gccccacgac cacataactc tgggcactat tctattgaag cctgtgattt ctctcagttt
    13141 gacacccata ttctcggtgt tctcggagca ccattaccag tcatcaaact ccatgcgcca
    13201 gccgttatgc ttaatgtcct cggtcagcat gtcgaggctg ctgagcaata tgtgacagaa
    13261 aattcaagcg cccacctcca catgtatggt aaaatagaag cgaagcacaa ccgcaagatg
    13321 ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg atgagcttgg ggaagggatt
    13381 gatttttagg acaagtctat gatacaaatt atcgttaata catttattga aaaggataag
    13441 actggagcag ttgttgaagt gttgtatgcc agtgctgacc aagataaggt acaagctaaa
    13501 tatgaagaac tagctgcaca ataccccgaa aattatttag ctatctatga tgtaccgctg
    13561 gatacggatt tgaatacact agatcattac ccgtctgtgt ttattgaaaa agaggagttt
    13621 gagtagaaat cttggtttac ctagatagct tattcccaac agcttaagaa gaaaggaaaa
    13681 attaacacat gatcaaccgt tactctcgcc ctgagatggc gaatatttgg agtgaagaaa
    13741 ataaataccg tgcttggctt gaggtggaaa tcctctctga cgaggcatgg gctgagttgg
    13801 gggaaatccc taaggaagat gtggctttga ttcgcaagaa ggcggacttt gacatcgacc
    13861 gtattttgga aattgagcag gagacgcgcc acgatgtggt ggctttcacg cgtgcggttt
    13921 ctgagactct tggtgaagag cgcaagtggg ttcactatgg gttaacttct accgacgtgg
    13981 tggatactgc ttatggttac ctctacaagc aggccaacga catcatccgt cgtgaccttg
    14041 aaaacttcac taatatcatc gctgacaagg ccaaggagca caagttcacc atcatgatgg
    14101 ggcgtactca tggtgtgcac gctgagccga caacttttgg tcttaaattg gcaacttggt
    14161 atagcgaaat gaaacgcaat atcgagcgtt tcgagcatgc ggctgctggt gtagaagctg
    14221 gtaagatttc tggtgcggtt gggaactttg ccaatatccc accatttgta gaggagtatg
    14281 tctgcaacaa gcttggtatc cgtgctcaag aaatctctac acaagtcctt cctcgtgacc
    14341 ttcacgctga gtactttgca gttcttgcca gcattgcgac ttcaatcgaa cgtatggcga
    14401 ctgagattcg tggtctacaa aaatctgagc aacgcgaagt agaagagttc tttgctaaag
    14461 ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc aatcggttct gaaaatatga
    14521 ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc ttatgaaaac gtcgctctct
    14581 ggcacgaacg cgatatttcc cactcatcag ctgagcgtat catcacacca gatacgacca
    14641 ttttgattga ctacatgctc aaccgttttg gaaatatcgt caagaacttg acagtcttcc
    14701 cagaaaatat gatccgaaac atgaactcga cttttggtct tatctttagc caacgggcta
    14761 tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc ctatgacttg gtgcaaccaa
    14821 aaacagccta ctcttgggac aaccaagtag actttaaacc acttcttgag gcagattcag
    14881 aagtaacatc acgtctcact caagaagaaa tcgatgaaat cttcaaccca gtttattaca
    14941 ccaaacgagt ggatgatatc tttgaacgtc ttggactagg tgattaatta aaaaataaac
    15001 agcgagcttc aatctcgctg tttatttttt atcgaaaaga cttagtcttc ttttctttta
    15061 gtgagtccat aggctgctag tgtggacatg agtcctgcga ctactagttc tgcagaatcg
    15121 tgagttcctg tttcaggaag ttttttctct gttaccacag gagctggatc ttgaggaaga
    15181 gctttgcttt cctcagcagg agcagttgat ggagctggtt ggcttgggat ttctagtttt
    15241 ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc ctacatgtgt taccatagtt
    15301 ccgacttcga ctatttgagt aacggcttcc tgtgctacga cactatttac aagtgttttc
    15361 acttccttac catcggcaga agtgctcaca gagtagaagt tgctacgatg tccattgacg
    15421 cccttagtaa tgacttgtgt ttttcctttg agtaagagtg gattttcacg agtcactgtg
    15481 gtaaatggaa tttcttcttc ttggatatcc agtctaggtt ttacctcagt agttggtgca
    15541 agaccacttt catcaccctt gtgagttaca ggagagccaa cttcaaccac ttggtttata
    15601 acttctttgg ttacctggct atcaaggact gtttctgttg tttttccatt ttcagtgagt
    15661 acagagatgt aatgagttcg ttcacctttg actcctgctg tgataatatt ttcctgacca
    15721 gctgggaggt taggattttc tttcttgata acttcaaatg gaatttcttc agttcttgtg
    15781 atgagttctg gtctggtttc aacattggca gccacttcat tttcatctag gcttcctgaa
    15841 tgagttgcag ctggtttgag gccttgaaga gcggctttta ggttggctac aagtgtgtca
    15901 agctcagctt gtttattacg gttgaggttg taatttagag ctgttttagc tgcgtcaagg
    15961 gcctcaagac tttctttact atatccttct aagtttgtag gaattttagc taattcttcg
    16021 cggagagcat tataattagc acggaagtag tctttgttgt ggtctgcaaa ggcagtcatg
    16081 agttcaaaga tttcctcttc cttgtactca gcgcttggtc tatctgccca gattgaaagc
    16141 atacttccga ctgttggaag atctacttca ggatatttgg tagaagctag ttgattgaat
    16201 ggaactttac cagaattttc aactgctttt gaaagagggt aagcttcatc ttgtttgtga
    16261 ttgcctataa cataatacca gtcaccgttg gtattcaaga atttatagcc tttgcttgct
    16321 aggtattgag gtgatgcgag gttatatccc caccagcctt tagaccagta agaaatcaag
    16381 acatctttgt caaactgaac atcgtccttg tcttcatagt agaagccatc gttgaaggcc
    16441 attggttgaa gccctctttc tttggccata gctgcgaggg tgttggcata ttcggcaaat
    16501 ttgccataga gttgatacca cttgaggtag taccagcctt gggcactagt cgcatcgttg
    16561 gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac ctgcaaagaa gtccatgtat
    16621 ttaccgatga gggcttttac aaagttcatc gcttcttcgt ttttcaagtc catagttgtt
    16681 tttgaaactt tatcaaagtg ggcttgagga tttttaatac ctaatttttc catggcaacc
    16741 agcatagcat ccatgtgacc tggactgtta atagctggga tgagaccaat gtccttagat
    16801 ttagcgtatt caattagctc tgtcacttct gcctgtgtta gtgcagtacc gtttggatcg
    16861 tcgtagtaag ctttagttcc ttcgataata gcttttttaa cgtcatcact agcataggtt
    16921 tttccgttgg cagtaatggt catatcattg agtagaaagc gaagtccgtc atttcctaga
    16981 aggagatgga catcagaata tccgagctca ctggccttgt ctacgatgcg tttgagctgg
    17041 tccagagtaa agtatttgcg tccagcatcg attgagatta ccttgttttt ggcaagtttt
    17101 tcaacctcac gtttagcttc ttcttctttt tgagcttcag gcgtgagggt caagttgttg
    17161 acagtttctt gaagtttagc aatggcttgt tcaatagtat cttgttgggc acggctaagg
    17221 ttgctatcga gagagcgaat agctttttca gcttctttta cggccgcgac gctttctgca
    17281 gtataacggt tcaggtcttt tggtacctcg ttaagtgctt gctctgcaga ttcataatca
    17341 gctgcgaagt attcagcgtt ggcatttgca aaatgacgca tgagtttgaa gaggtgtgat
    17401 ggtgaataac gtgcagatgg agtgtcagcc caagcagcta ccataccacc gatgattggg
    17461 atatcagctc cttctgtttt tggtacagaa gtgattggtg tgtttttaat accattgagc
    17521 ccctgatcga gattgtacca gccttggccg tcagcgtttc gtccaagaac gtagtaccaa
    17581 gcatcattgg tattaaggat ttggtgacct ttttcagcta gtagtttaga agaagcgaca
    17641 tcgtagcctc cccaaccacc agtccacata gaaacgatga tgtctttgtc aaaactacca
    17701 aagcttgtgt cgctattgta gtagataccg tcgttaaagg ccattggttt gagaccgtgc
    17761 gattttacaa tacgagcgag gtcattggcg taggcaataa atttttcata gccttttaca
    17821 gggtagcctt cgtttggata gtatttatca gcttgaagca cactccaacc tttagcatct
    17881 gtcgcatcat tggcatattc atcaagtccg atgttgaaga tttcagtctt tttcgcgaaa
    17941 taagcagcat acttgtcgat aagggctttt gtaaaagcga cagcttgttc gttgtcaaga
    18001 tcgacagtac gggctgattt cttcccaaaa tagctaaagt tagggttttg gattcccaat
    18061 tctttcatgg cattgagaat cgcatccatg tgtccaggac tatttactgt cggaatgaga
    18121 ccgatacctt tatctttggc atagttaatc agatctgtca tttgactttc tgttaagtga
    18181 ttgccgtttg gatcgttgta ataatcattt gtaccttttt caatggcgcg tttgacatcg
    18241 tcactggcat aggtcttgcc gttagctgtg atgctcatat cgtccaacat gaaacggagt
    18301 ccatcatttc cgactaatag gtgtaaatca gtgtagccat aatgtttcgc tttatcgatg
    18361 atttccttga gctgttctgg tgagaaatat ttacgtccag catcaataga aacaattttc
    18421 tttttcgcta gtttttcatt tacagttgca gcacgttcct ttcctgcctc tgtttccggt
    18481 ttgtcagcct ctgctttcgc ttcatctttt ttagctggtt tatccttgtc agtcttgtct
    18541 gtatttgact ctttagaatc aacctctttc gcttcttcct ttttagggct agcttcttct
    18601 gcctttttat tagcagtttc tttttcagca gaagttggag ttaccacctc tgctttatca
    18661 ctaggagttg aactaacttc ctcttgtggt ttttcttctg tttttggaag actagctacc
    18721 ttatcagtag ctggagtttc tgtttctaca gtttttggag cttctggttg aagctctgct
    18781 ttaggtgttt cctcagtccg attttcggat gattgagggg aatcagaaac tgtatggatg
    18841 gtcggttggt tttctgtagt agtagtagta actccatcgg ctgcaacagt ctgtgcttgg
    18901 aaggcaaatc caattagaac agaagctgct cctacagcgt atttacgaat agaaaaacgc
    18961 tgttgttttt catgtttcat tgcaaaacct cctgattgca ttgttatatt gatagcgatt
    19021 atataaatca acgcccttat tttatttctt atattaacga gagtcaagag g
//
LOCUS       DAVMEF010000023        18756 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMEF010000023 DAVMEF010000000
VERSION     DAVMEF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.023"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(160..966)
                     /gene="yidA"
                     /locus_tag="VYQ30_000575"
     CDS             complement(160..966)
                     /gene="yidA"
                     /locus_tag="VYQ30_000575"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1965737.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMADKEIVKCMFIDEPE
                     ILDATIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(979..2283)
                     /locus_tag="VYQ30_000576"
     CDS             complement(979..2283)
                     /locus_tag="VYQ30_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1965738.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQIVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2355..2732
                     /locus_tag="VYQ30_000577"
     CDS             2355..2732
                     /locus_tag="VYQ30_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1965739.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2823..3155
                     /locus_tag="VYQ30_000578"
     CDS             2823..3155
                     /locus_tag="VYQ30_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1965740.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3167..4738
                     /gene="ffh"
                     /locus_tag="VYQ30_000579"
     CDS             3167..4738
                     /gene="ffh"
                     /locus_tag="VYQ30_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1965741.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4933..6216)
                     /locus_tag="VYQ30_000580"
     CDS             complement(4933..6216)
                     /locus_tag="VYQ30_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1965742.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6568..7281)
                     /gene="rsmG"
                     /locus_tag="VYQ30_000581"
     CDS             complement(6568..7281)
                     /gene="rsmG"
                     /locus_tag="VYQ30_000581"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1965743.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7375..7935
                     /locus_tag="VYQ30_000582"
     CDS             7375..7935
                     /locus_tag="VYQ30_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1965744.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7937..8836
                     /gene="htpX"
                     /locus_tag="VYQ30_000583"
     CDS             7937..8836
                     /gene="htpX"
                     /locus_tag="VYQ30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1965745.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8985..10544)
                     /locus_tag="VYQ30_000584"
     CDS             complement(8985..10544)
                     /locus_tag="VYQ30_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1965746.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10783..11100)
                     /locus_tag="VYQ30_000585"
     CDS             complement(10783..11100)
                     /locus_tag="VYQ30_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1965747.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            11270..11812
                     /locus_tag="VYQ30_000586"
     CDS             11270..11812
                     /locus_tag="VYQ30_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1965748.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11812..12441
                     /gene="nth"
                     /locus_tag="VYQ30_000587"
     CDS             11812..12441
                     /gene="nth"
                     /locus_tag="VYQ30_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1965749.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12652..13173
                     /gene="pyrR"
                     /locus_tag="VYQ30_000588"
     CDS             12652..13173
                     /gene="pyrR"
                     /locus_tag="VYQ30_000588"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1965750.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13192..14115
                     /locus_tag="VYQ30_000589"
     CDS             13192..14115
                     /locus_tag="VYQ30_000589"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1965751.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14165..15244
                     /gene="carA"
                     /locus_tag="VYQ30_000590"
     CDS             14165..15244
                     /gene="carA"
                     /locus_tag="VYQ30_000590"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1965752.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15557..18733
                     /gene="carB"
                     /locus_tag="VYQ30_000591"
     CDS             15557..18733
                     /gene="carB"
                     /locus_tag="VYQ30_000591"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1965753.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5448 a         3624 c         4204 g         5480 t
ORIGIN      
        1 tacaattcag taccacaaat taagaaagca atgcctattt tttgtactct caagatttaa
       61 gtaactgtac aactgtaatt aaatttatta atttactctc attgtgatat attcatacaa
      121 aaaccgctca gctgagcggt tttagtctat tctttccttt tacagtaccc atgttcggat
      181 ggcgtgggca acgccggatt cgtcatttgt tttagtgatg tatttggcga tttttttgat
      241 ttctggattt ccattttcca tgacaacggg gtttccaacg acttccagca tggcacggtc
      301 attttcttcg tcaccgattg ccatggtttc atctttggtc aatccgagtt tttcagccaa
      361 gtgagtaatg gctgaaccct tgtctacatt ctttgtaagg agttcgaggt agaaaggagc
      421 agatttgttg atggagtagc gctcgtaaaa ttctgctgga attttttcaa tcgtagcatc
      481 gaggatttct ggttcatcga tgaacataca tttgacaatt tccttgtcag ccatttcttc
      541 aggtgtacgg tagaagatag gcatgctgac gagggttgat tcgtgcacag tgtattttcc
      601 gatattgcga ttggcagtat agataccgtc cttggtaatg gcgtgcatgt ggacaccgag
      661 cttgcggctg agaaattcca tatctagata atcctcataa gtcaaggatt cgctgataat
      721 ctcatgtcct gtagcagttt cttggacaag ggcaccgttg aaggttacca catagtcccc
      781 ctcgtctctc aactgcaagt cgtctagaag tttggcaacg cctgcgatag ggcggccagt
      841 tgcaatcacg actttgacac cagcttcttt ggcatcttgg atggcagaaa agacttcagg
      901 agtgatttcc ttttggctgt tgactagggt tccgtcaata tcgacggcga ttagtttaat
      961 actcataaat ttcctctttt agtttttatt tggggtaaaa tgatcgttct caatcaagtg
     1021 taaaaattgc tgggtgatgc ttgcgaagat gctgttttgg tctaacattt ctttcgggaa
     1081 ataaaagcga ttatctccgt ggcgacttcc agcaagggat tggacgatag gggacaggct
     1141 agagagttct gccagttctc catttttttg taaaatctca atctgtgtcc ttggattttc
     1201 agattcggga cggtaaatat cataagggag gtcaaagttc ttatgaatgg cagtatagta
     1261 gtcgggatca aagccgatgt cctcaaccaa ttttctcatg ctagcgagtt ggtcttggtc
     1321 ttcttgtgaa aaggtaatgg atttaaagac cttgcggttg acaaagcgat gcgataaatc
     1381 tgcaagaatc ttgtcaggac tggtcatcca gagttggaag taggtattca tcacaccatc
     1441 atccagagcc agatagtcag tcaaggtcac atttttttcg aagaaaggca ggaggtgtgg
     1501 agaagttcgg gcaaagaaat ccttgtcctc aggatagagt tccttggcgc gtttgagaag
     1561 attctgtagg agaacttcca tggcgcgtgt tgcggggtgg aaataaacct gcatgtacat
     1621 ctggtagcga ctgaggacgt agtcttcgat ggcgtgcatg ccattgcgct gaaaggcgat
     1681 accattttcg ataggacgaa tgactcggag gattcgagtc aggtcaaatt ccccatagga
     1741 tgctcctgta aaataggagt cgcgcaagag atagtccatg cgatctgcgt caatctgact
     1801 agaaatgagc tgcacgatct gcttgttagg ataggtatgg tctatgacac tggcaacttt
     1861 ctctggaaaa tcgggcgcta cttgtagcaa gacttggtga atctctgtct caggattttg
     1921 aataatctcc tgagtaatgg cttcatggtc tgtatcaaag agattttcaa aagtatggga
     1981 gtaggcacca tgcccaaggt cgtgtaggag agcagcggtc atggtcaaga gagactcggc
     2041 aggattccat tcctcaggat atttttcttc gaaaatctct gtgatgcgtc gtgcaatttc
     2101 atagactcct agacagtgag agaagcgact gtgttctcca ccgtggaagg tataactgga
     2161 agttcccagt tgtttgatcc ggcgcaaacg ctgaaattct tttgtattaa tcaagtcata
     2221 gatgatttga ttattgacat ggatgtagtt gtgaacaggg tcacggaata ctttttcgtt
     2281 catatgacca ttatagcaaa atcctgctct ttttggtaaa atagtaggac aagagacaag
     2341 aaagagggcc tgatgtgaag attcggatgc gaaatacaat tcagtttgat gagcagttgg
     2401 aagtgattga ccagctttat gatgtggaag tgcatgaaaa aggagattat agctacctgc
     2461 ttttctataa tgaggaaaag gaaaaagtag tgattaaatt tcatggtcaa gaacttatga
     2521 tgagccgatt ttctaatccc aagaccatta tgcgctttct aaaggatagc gatagtttag
     2581 cctatattcc tacacctatg ggcatgcagg agtttatcat ccaaacgagc cattatcaag
     2641 ttgataggca aaagattgaa ctagattatc aactacaaaa tcaagaggga catccttttg
     2701 ccagctatca attggaaatt acttggggct aggctcatgt ctttttcaaa tttagctatc
     2761 tatcttcttg gatcggcttc ctagtgtggt agattaggct aatttttggt ataataaaag
     2821 ttatggaaat cgaaaaaacc aatcgtatga atgcgctctt tgaattttat gcggcgcttt
     2881 tgacagataa gcaaatgaat tatatcgagc tctactacgc tgatgattac agccttgctg
     2941 aaattgccga ggagttcggt gtcagtcgtc aggctgtcta tgacaatatc aagcgaacag
     3001 aaaagattct ggaagattat gagatgaaat tgcacatgta ctcggattat attgtccgca
     3061 gtcagatttt tgatcagatt ttggagcgct atcccaagga taactttctg caggagcaga
     3121 tagaaatttt aacaagcatt gataatagag aataagagga agaaaaatgg catttgaaag
     3181 tttaacagaa cgtttgcaga acgtctttaa aagtctacgt aaaaaaggaa aaatctctga
     3241 atctgatgtc caagaggcaa ccaaagaaat tcgcttggcc ttgctcgagg ccgacgttgc
     3301 cttgcctgtt gtaaaggact ttatcaagaa agttcgtgag cgtgcagtcg ggcatgaggt
     3361 cattgataca cttaatcctg cgcaacagat tattaaaatc gttgatgagg aactgacagc
     3421 tgttttaggt tctgatacgg cagaaattat caagtcacct aagattccta caatcatcat
     3481 gatggttggt ttgcagggag ctggtaaaac aacttttgca ggtaagctgg ctaacaaact
     3541 caagaaggaa gaaaatgccc gtcctttgat gattgcggcg gatatttatc gtccagctgc
     3601 cattgaccag cttaagacct tgggacaaca gattgatgtg cctgtctttg cacttggaac
     3661 agaagtacca gctgttgaga ttgtacgtca aggtttggag caagcccaaa ctaatcataa
     3721 cgactatgtc ttgattgata ctgcgggtcg tttgcagatt gatgagctcc tcatgaatga
     3781 gcttcgtgat gtgaaagcat tggctcaacc aaatgaaatc ttgcttgtcg ttgatgctat
     3841 gattggtcag gaagcagcca atgttgcgcg tgagtttaat gctcagttgg aagtgactgg
     3901 ggtcatcctt accaagattg atggcgatac tcgtggtggt gctgctctgt ctgttcgtca
     3961 cattactgga aaaccaatca agttcactgg tacaggtgaa aagattacgg acattgaaac
     4021 cttccaccca gaccgcatgt ctagccgtat ccttggtatg ggggatatgc tcactttgat
     4081 tgagaaagct tctcaggaat acgatgaaca aaaagccctt gaaatggctg agaagatgcg
     4141 cgaaaacacc tttgatttta atgatttcat cgatcaatta gatcaggtgc aaaatatggg
     4201 gccgatggaa gacttgctca agatgattcc aggtatggcc aacaatccag cccttcaaaa
     4261 catgaaggtg gatgaacgcc agattgctcg taaacgtgcc attgtgtctt cgatgacacc
     4321 tgaagagcgt gaaaacccag atttgttaaa tccaagccgt cgccgtcgta ttgctgctgg
     4381 ttctggaaat acattcgtcg aagtcaataa attcatcaag gactttaacc aggctaaaca
     4441 gctcatgcag ggtgttatgt ctggggatat gaataaaatg atgaagcaaa tggggattaa
     4501 tccaaataac cttcctaaaa atatgccaaa tatgggagga atggatatgt ctgcccttga
     4561 aggaatgatg ggacaaggcg gtatgcctga cttatcagct ctcggaggag caggaatgcc
     4621 agatatgagc cagatgtttg gtggcggttt gaaaggtaaa attggtgaat ttgccatgaa
     4681 acagtccatg aaacgtatgg ctaacaaaat gaagaaagcg aagaagaaac gcaagtaaga
     4741 caagagattg atgcaaatct catttgatga gaaaataaag taaaaatttc tatgataaaa
     4801 cgtacagtat caaggtttta gaatatctga tattatacgt tttttgattt taataatttt
     4861 gttaagacac ttttattatg gtatgcgaaa aatccactca gctgtctgag tggattaggt
     4921 gtatttagac tcttagtctt tattttcgta tggcaagatc aagttcaaga tgattcctgt
     4981 catggctgaa agggcagtac ctgaaagtgt aactggacca agtttaagga tagctcctcc
     5041 aagtccaaga accaacatag cacttgcgat gatgaggttt cgcatttgag cgaaatcaac
     5101 acgttcttta atcaagactt tcaaaccatt gctggcgata accccataga gaaggattga
     5161 cataccacca agtacagcgt ttggaatagt tgaaatcaag gcagtgaatt taccaaggaa
     5221 gctgagggca atcgcgatga aggcagcgtt acggataact gagacagaag cgatacgagt
     5281 cataccgata acccctgtat tttctccgta agttgtattg gctggtccac caaggaaggc
     5341 agaaacagaa gttgcgatac cgtcaccaag aagagtacga tgaagacctg gttcttttaa
     5401 gaattgacgg ccacagattt gacccaaaac agtatggtct ccgatatgtt cagaaattgt
     5461 tacgatagcg attggcaaga tagcgatggc ttctggacca aagtaaagat tgtactcttt
     5521 aaaggcacca cctgtgctaa atggcaagta gaaaccagga atttcgaacc agttggcttt
     5581 aagaactggt gtaaagtcaa ccaagccaag agttagtgcg aaaaggtaac caccgataat
     5641 ggcaaagagg aatggaatga ttcgtaggaa gccttttcct tttgtattga taaaggcagc
     5701 aattaggaaa gtaacaacgg ctaccagagc atttttccaa tttccgtctg ctacaagacc
     5761 tgcattggta acagctgaac ctgcaagtcc aagaccgata acgatgatca taggaccgat
     5821 aatgattggt ggcaagagtt tatcaatcca ttttgttcct acaaatcgga tgctggtagc
     5881 aacaaggaca tagaccaaac cagtcaagat aacccctgtt tgggcagcag atacatcacc
     5941 ccccatttct ttcatagcca gtgacatagc tgtgataaag gcaaatgaag aacctagata
     6001 aactggaact ttaaaaccag tagcaatcat gtagatgagt gttccaacac ctgaagcaaa
     6061 aagggcaaca gatacaggca ttcccaaaat caatggtacc aagatggtcg caccaaacat
     6121 ggcgaaaacg tgttggaagc taaggagaat tccttttcca tccgaaggac gttggtcaac
     6181 gtctagtaac aaatcaacag ttgattcttg tttcatataa acctcacttt tgggcatcag
     6241 aaaagagccc attattttac agcaataggc cctcagagta agacttctct aaaatctaga
     6301 ctttaaagag tttcaaatat ttttgcgtct tcgtcacctc acgggatgac attaaaaacc
     6361 tttgcttagg atagtatagc agaaaaaaat gattttgtaa atcatttttt cccgagccta
     6421 gaaataaaag agcgaggctg attttgtaaa tcattttttc ccgagcctag aaataaaaga
     6481 gcgaggctga ttttgtaaat cattttttcc cgagcctaaa aataagagag cgaggttgat
     6541 tttgtaaata tttttttact taaaaattta aagcgggcgt ttatttggca taccagcctt
     6601 acgtggatat ttatttggtg tttctttttt cttttctacc actgtgatgt aacgcggatc
     6661 tctatttggt agggcgtagc tgagattgtc ttcgacttta ctaaaaagga gattgagggc
     6721 attcttagct tctaacaact cctcaggcgc attgctggcc ttgagtgcca atagttttcc
     6781 accaacctta aggtagggaa tagtcaattc agataggacc tgcatacggg caaccgcacg
     6841 agctgttaca aaatcatatt gagcacggaa gttcttgtct tgggcaaaat cttcggcacg
     6901 tccgtggtag aaatgaactc cgttcaaatc cagttcttga gccaagagtt gtaggaagtt
     6961 gatgcgctta ttgagtgaat caataatggt cacatctaac tccggataga gaattttcat
     7021 tggtagacta ggaaatcctg ccccagcccc gatatcaaga agtttgatag tttcattggg
     7081 aatcaaacct tgaagaatgg gtgcaatcga atcgtaaaaa tgtttgagat aaacttcttc
     7141 cttgtccgta atcgccgtca aattaatctt ctcattccac tcgaccaaga gctcaaaata
     7201 acgttcaaat tgttcttttt gctggttcga aagtggaaga ttctgctcgg caagcaagtt
     7261 gtaaaatgtt tctggtttca tagttatttc cttctttagt atcatcttat tttaccacaa
     7321 tcattaaaaa tttgatatac tggtactaat ctaaaagtag aaaggactta tatcatgact
     7381 tggattattc ttggagttat cgctcttatt gttatttttg tgattgttag ctataacggt
     7441 ttggttaaaa atcgtatgca aaccaaggag gcttggagtc agattgatgt tcagttgaaa
     7501 cgtcgcaatg atctcttgcc aaacttgatt gagactgtaa aaggttatgc caaatatgaa
     7561 ggttctaccc ttgaaaaggt ggcagaacta cgtaaccaag tggcggcagc gacttcacca
     7621 gcagaagcta tgaaagccag tgatgccctc actcgtcagg tttcaggtat ttttgcagtt
     7681 gcagaaagct atccagattt gaaagctagt gctaactttg ttaaattgca agaggagttg
     7741 acaaacacag aaaataaaat ttcttactct cgtcaactct ataacagtgt tgtcagcaac
     7801 tacaatgtaa aattagaaac tttcccgagc aatattatcg ctggaatgtt tggatttaaa
     7861 gcggcagatt tccttcaaac acctgaagag gaaaagtcgg ttcctaaagt tgattttagc
     7921 ggtttaggtg actaagatgt tgtttgatca aattgcaagc aataaacgaa aaacctggat
     7981 tttgttgctg gtatttttcc tactcttagc tcttgttggt tatgcggttg gttatctctt
     8041 tataagatct ggacttggtg gtttggttat cgcactgatt atcggcttta tctacgcttt
     8101 gtctatgatt tttcaatcga cagagattgt catgtccatg aatggagcgc gtgaggtgga
     8161 tgagcaaacg gcaccagacc tctaccatgt agtggaagat atggctctgg tcgctcagat
     8221 tcctatgccc cgtgttttca tcattgatga tccagcctta aatgcctttg cgacaggttc
     8281 taatcctcaa aatgcggctg ttgctgcgac ttcaggtcta ctagctatca tgaatcgtga
     8341 agaactagaa gctgttatgg gacatgaagt cagtcatatt cgtaattatg atatccgtat
     8401 ttcgactatt gcagttgccc ttgttagtgc tatcaccatg ctttctggta tggcaggtcg
     8461 tatgatgtgg tggggtggag caggtcgcag acgaagtgat gatgaccgag atggaaatgg
     8521 tcttgaaatc attatgctag tggtttccct actagctatt gtactggcac ctctcgctgc
     8581 aaccttggtt cagctcgcta tttctcgtca gagggaattt ctggcagatg catctagtgt
     8641 cgagctgact cgcaatcccc agggaatgat taatgcccta gataagttgg acaatagcaa
     8701 acctatgagt cgccacgtcg atgatgctag cagtgctctt tatatcaatg atcccaagaa
     8761 aggtgggggg ttccaaaaac tcttttatac ccacccacct atctcagaac ggattgaacg
     8821 tttaaaacag atgtaaaatg aaggctggaa aaaagtcttt aaaatctgaa aaacgcataa
     8881 tatcaggtgt gaaaacttga tattatgcgt tttactatgg gaagatttac ttctttttct
     8941 cctaaaattg tgtttttgcc ccacctatct gctatgttgc aaatttataa atcttctaaa
     9001 ttaactagct tcaaaccgtg ttctgtcatg cgatagatga tcgagcagat ttcttttaag
     9061 aaacgacggt catgcgaaac agtgatgaga ccgcctggat aggttgcaaa gagttttctg
     9121 atttggggtt gagaagtggg agaaaagttt cgtgtgggtt catccagcag gagaaagttt
     9181 ggtttgcgca ggactaaatc caaaagcagg agttttccct gttgtccgcc agataaggag
     9241 cgaatttgat gctgcatttc tggataactg aaattgagac tggctaggtg agattggatt
     9301 ttctgaagtt cctctttttc cccagttttg ctgatataag ctattggtga taaatccaat
     9361 tgcagttttt tgtggtaatc ttgtggcata aaaccaaggg aaatctctct tttatcattc
     9421 agaagttgct ttaatttggc taacagagtt gatttcccaa caccatttgg cccgataata
     9481 ccgatttttt cttgcccacg gacagttagt tgtagttttt gaaccaaaac tcggtcgtca
     9541 atggacaaat tttctttttc cagttggact aagactttag aagctggtaa tggttggatg
     9601 tctgaaaaga aaagttggat ttgttcctct tcaagtggct tttgagtcat ggactgagct
     9661 gtcttttcgt agcgtttttc ttgtgagagg acagttttca tctttttagc caataggcga
     9721 ccggcagtac tatctttggt agctcgaagc gcagtttcta cattttgctt aactctccga
     9781 tgtttttcca tggttttatc gtaggctctt tggttgttag cagcttgctg actttgtttg
     9841 gcaaaattag cctttctctg ctcactatag ctatcatagt ctaaatgctc tactagcgtt
     9901 tccgcttctt tacggtgttt gaccagtcgc aagtgaacaa tagtgtctgc tgtttcagaa
     9961 agaaagtctt catcatggga aatgaaaata acggtttgcc tggtcttttg aatctggcct
    10021 tttagccaat caactgtctc aaggtctagg tcatttgaag gttcatctaa aaatagaatc
    10081 tcaaagggtt tggctaactc atggataagc tgaattttca aagcttctcc ccctgataga
    10141 ttgccaatct tttggtcact tgcgaaacga ttgctatcaa aatgcaattc ctccgccaaa
    10201 cgatagagga tactgtagtc taaatcaata gaatctaaaa agaagtagtc gtgtaaagtt
    10261 ttctttttca gctcctcggg aagtttttga ggaatgtagg ccagtgactg atagtcagat
    10321 tggatgtttc ccttgatagt gaaatcagac aaagcttccc ccattaaaat tttaagtaag
    10381 gttgatttgc catttccttc ttcaccaata atagcaacct tttccccgtc ttggatggtc
    10441 atgcttaggt cagatacaag atctcgtaaa tctttgtttt gtgtgatggt tagatgattg
    10501 atttttatca tatgatttcc ctttctagaa tgttcatagt gcataacaaa aagctctact
    10561 ttacctagta gagcccaaag cctatgtcaa aaaaactcta ttaacatgcc agaaaaagag
    10621 cataaaagag cacacaaaaa gagacaaaaa caagatctac taaataaagg ttctttattt
    10681 gatagactta gttgttcatg tctcccttac ctccgagtat tagtatcttt atattatacc
    10741 tttaaggaaa ttttgtcaac agaaaactgg aaattctatg ctctaaaatc caaaaacagg
    10801 tccatagaga cttagtacgt gtttgacatg ctcgtaatgg aatttatcat agtttcgcca
    10861 agcagtgcgt gcttgatcgt ttagttttag gctacccagt ccaatcagaa gactggtggg
    10921 ttggtaaaat ttctcaagat caattaagat actgttgttc aacttttccg tttttttgag
    10981 ctcttgcata gtagtgttta gcagctcttg tagtcgaata tcatctgccg taccctgttt
    11041 acagttttgg tagcttttat ttaacttgga taggagttga atgagtttct tgtcttccat
    11101 gtgagcatcc tccttgatct gatgtactct ttccctatag tatagcacta aaaaacaaaa
    11161 ctgtcatagt aaatatgtta aaattctgaa ttttaattac aaagtttggt gtgctgattt
    11221 gtagcctttt catggtataa tcaagagagt aaatctttat ggaggaagta tgaaattaaa
    11281 tattcaagaa attcgtaagc agtctgaagg tttgaacttt gaacaaacgt tagatttagt
    11341 tgatgacctg cgtgcacgta atcaagaaat tttagatgta aaagatatcc ttgcagttgg
    11401 gaaagtacaa tatgaagacc gtatgtattt cttagattat caactatctt ataccattgt
    11461 tcttgcttcg agtcgcagta tggagccagt tgagttagtt gaatcttatc cagtcacgga
    11521 agttttcatg gaaggcgcaa ctaaccatct aaatcaagaa attttagatg atgacttggt
    11581 cttgcccatc gaaaatgagg cgcttgacct tgctgagagt gtatcagaca atatcttgct
    11641 aaacattcct atcaaggtct tgacggctga agaagaagct ggtcaaggat ttgtctcagg
    11701 aaatgactgg caaatcatga cagaggaaga ataccaagct caacaagcag taaagaaaga
    11761 agaaaacagt ccttttgctg gcttacaagg actatttgac ggagatgaat aatggtcttg
    11821 tcaaaaaaac gagcacgaaa ggtgctagaa gaaatcattg ccctcttccc agatgccaag
    11881 cctagtcttg attttaccaa tcattttgaa ctcctggttg cggtcatgtt atcagcccag
    11941 acgacagatg cagcggtaaa taaggccaca ccaggtctct ttgttgcctt tccaacaccc
    12001 caagctatgt ctgtagcgac agagagtgag attgcttcac atatttctcg cttgggattg
    12061 tatcggaata aagctaaatt ccttaaaaaa tgtgcccaac agttactaga cgattttgat
    12121 ggtcaagtcc ctcagacacg tgaagaattg gagagtttgg caggtgttgg tcgcaagaca
    12181 gccaatgttg tcatgagtgt aggatttggg attccagcct ttgcagtgga tactcatgtg
    12241 gagcgtattt gcaaacacca cgatattgtc aaaaaatcag cgacgccact tgaggtggaa
    12301 aagcgggtca tggatatctt gccgcctgag cagtggttag ctgcccatca ggccatgatt
    12361 tattttggaa gagccatttg ccatccaaaa aatccagagt gtgaccagta cccacaatta
    12421 tatgatttta gcaatttgta agatgctaca ttgaaacttt ttgcttgatt ttaagcatgc
    12481 tttgtgctat agtaaatgat aactaaatat tcctttaaac ctgtcccgtg aggcaggcaa
    12541 ggagcgcgat aaagtagaag ggtgaagtct atactgtgtg cagtagggct cgcttggcta
    12601 taaatttcaa gtctccttgt ttaaggagac ttttcttttt ggaggtttgt catgaaaaca
    12661 aaagaagttg tagacgaatt gaccgtcaaa cgagcgatta cgcgtattac ttatgagatt
    12721 atcgaacgca acaaagattt gaataagatc gtcttggctg gtattaaaac tcgtggtgtc
    12781 tttattgccc accgaatcca agaacgtttg aagcagttag aaaatctttc agttcctgtt
    12841 gtggaattgg atactaaacc tttccgtgat gatgttaaaa gtggagaaga tacttctttg
    12901 gtttctgtcg atgtgacaga ccgcgaagtt atcttggtgg atgatgtgct ctatacaggt
    12961 cgtaccatcc gtgctgctat tgataatatt gtcggtcatg gtcgtcctgc gcgtgtgagt
    13021 ttagcagttc tagtcgatcg tggacataga gaattgccaa tccgtccaga ttacgttgga
    13081 aaaaatatcc caaccagtcg ttctgaagaa atcatcgtag agatgacaga acttgatgac
    13141 caagacagag ttctgattac tgaagaagct tagaaagtta aaggagtagc catgtcagaa
    13201 aatcaacaag cattgaacca tgtggtgtcc atggaagacc tcactgtcga tcaagtaatg
    13261 aaattgatca agcgaggaat tgagtttaaa aatggagccc agcttcccta tgaagaccat
    13321 ccgattgttt ccaatctctt ctttgaggat tctacacgga cacataagtc ctttgaagtc
    13381 gcagagatta aacttggatt ggaacgactt gactttgatg tgaagactag ttcggttaat
    13441 aagggtgaga cactttatga caccattttg actctgtctg ctttaggagt ggatgtctgt
    13501 gtgattcgcc atccagaggt cgactactat agagaattga ttgctagtcc aacgattacg
    13561 acttccatca tcaatggtgg agatggttcg ggccaacacc ctagccagag cttgcttgat
    13621 ttgatgacca tttatgagga atttggccac tttgagggtc ttaaagttgc tattgcaggt
    13681 gacttggacc actcacgcgt tgccaaatcc aatatgcaga ttttgaaacg cttgggatct
    13741 gaactctttt tcgctggacc tgaggaatgg agaagtcaag agtttgcaga ctatggacag
    13801 tttgtaacca ttgatgaaat cattgatcag gttgatgtca tgatgtttct ccgtgtgcaa
    13861 cacgaacgcc atgatagtgg agctgtattt tcaaaagaag actaccatgc ccaacatggc
    13921 ttgactcaag aacgttacga tcgtttgaaa gaaacagcaa tcctcatgca cccagctcca
    13981 atcaatcgtg atgtagaaat agcagaccac ttggttgaag caccaaaatc acggattgtc
    14041 caacaaatga ccaatggtgt ctttgttcga atggcaatct tagaatccgt actagcgagt
    14101 agaaacgcca actaaaacac aagacgttaa aagacgccag tgagcgtcca cgagaggtga
    14161 aaatatgaca aaaagaattc tagtattaga agatggcaca gtttttgaag gcaaggcctt
    14221 cggagcagat attgatgtaa caggcgaaat cgtctttaat acagggatga ccggctacca
    14281 agaatccatt acagaccagt cttataatgg acaaatcttg acctttactt atcctttggt
    14341 aggaaattat ggtattaatc gtgatgatta tgaatccatt attccaactt gtaagggagt
    14401 cgttgttttt gaagaagcgc gtagagctag caactggcgc aaccaaatga ccttggatga
    14461 atttttgaaa gccaagaaaa ttccagggat ttcaggaatt gatacgcgtg ctcttaccaa
    14521 gattatccgt aagcatggta ctatgcgtgc aaccttgacc catgttgggg acagtatgga
    14581 ccatgtgacg gaccagctcc aagcaacagt tttaccgaca gacaatatca aacaggtttc
    14641 tactaaaacg tcttatccag ctccaggagt tggtttgagc gtggtgctag tggactttgg
    14701 tctcaagcac tcaatcctac gtgaactttc taaacgcaac tgtaacgtga cagttgttcc
    14761 ttattcgaca acggctgaag aaattctcca tctcaatcct gacggagtta tgttgtcaaa
    14821 tggtccaggt aacccagaag acgttccaca agcactcgac atgattcgcg gtgtacaagg
    14881 aaaaattcca atctttggta tttgtatggg acaccaactc tttgcaatgg caaacggggc
    14941 taagacctat aagatgaagt ttggccaccg tggattcaac catgcggtac gtgaaatcgc
    15001 aacaggacga gtagacttca ccagccagaa ccatggttat gcagtcagcc gcgaggattt
    15061 gccagagcac ttgattatta cccacgaaga aatcaatgac aagtcagtgg aaggtgtgcg
    15121 ccacagatac caacctgctt tctctgttca ataccaccca gatgcagctc ctgggccaca
    15181 cgacgctagc tacctatttg acgagtttat cgagatgatg gaagttttta aacaatcaaa
    15241 ctaagaacga gtaaaagctc gtcggagaat ttatgtaact gaacacggac ggaaagctca
    15301 gaatgtagag aaaccaactt ggattgtcaa tccttccttg atttcctaaa attcaattgc
    15361 tttctattcg tcctttgtat cttatttaat gtcgccctga gaggcgacga atagaaagga
    15421 gaagggagct ccgtatttgc tgaactgaat acgggctacg gacggtgcta aaaagatagt
    15481 tattcctaga actgacagtt cttcgtcata actcctattt tagctttgtc cgcttgacac
    15541 ccttaatatc ttatatatgc ctaaacgtac tgatattcaa aaaattatgg tgattggttc
    15601 tggtccgatt attattggtc aggctgctga gtttgactac gctgggactc aggcttgctt
    15661 gtcgttgaaa gaggaaggtt atgaggttgt tttggttaac tcaaatcctg caaccatcat
    15721 gacggacaag gaaattgctg acaaggttta cattgaaccg attacacttg agtttgtgac
    15781 acgtattctt cgtaaggaag gtccagatgc cttgctacca acactcggtg gtcagacagg
    15841 tctcaacatg gccatggaat tatctaaaaa tggtatccta gacgagcttg gcgttgaact
    15901 tctgggtacc aaattatctg ccattgacca agcggaggac cgtgatctct ttaaacaatt
    15961 gatggaagag cttgaacaac ccattccaga atctgaaatt gttaacacag tagaagaagc
    16021 agttgccttt gcagcgacaa ttggctaccc agtcatcgtt cgtccagcct ttacccttgg
    16081 tggtactggt ggtggtatgt gtgccaacga gaaagaattg cgtgaaatca ctgaaaatgg
    16141 tttgaaattg tcacctgtta cccaatgttt gattgagcgt tcgattgccg gtttcaagga
    16201 aattgaatac gaagtgatgc gtgactcagc tgacaatgcc ttggttgttt gtaacatgga
    16261 aaactttgac ccagttggga ttcacacagg ggattccatt gtatttgccc ctgcgcaaac
    16321 catgtcagac tatgaaaacc aaatgcttcg tgatgcgagc ttgagcatta ttcgtgccct
    16381 caagattgaa ggtggatgta atgttcagct agctcttgat cctaacagct tcaagtatta
    16441 tgttatcgaa gtaaaccctc gtgtatcacg ttcgtcagcc cttgcttcta aagcgacagg
    16501 atatccgatt gctaagttgg ctgccaagat tgcagtaggt ttgaccttgg atgaggttat
    16561 aaacccagtt acaggttcaa cctatgccat gtttgaacca gcccttgact acgtagttgc
    16621 taagattcca cgtttcccat ttgacaagtt tgaaaagggt gaacgccgtc tcggtacaca
    16681 aatgaaggcg actggagaag tcatggcaat cggtcgtaac atcgaggaat ctcttctcaa
    16741 ggcctgccgc tcccttgaaa ttggggtgca ccacaatgaa atacctgaac ttgcagcagt
    16801 ttctgatgat accttgattg aaaaggttgt caaagcccaa gatgaccgtc tcttctacgt
    16861 atcagaagcc attcgtcgtg gctacacacc tgaagaaatt gcagagctta caaaaattga
    16921 tatcttctat cttgataaac tcttgcatat ctttgaaatt gagcaagaat tgggtgctca
    16981 tccacaagat ctagaagttt tgaaaacagc caaacttaat ggtttttcag accgtaagat
    17041 tgctgaactc tggggaacga ctgatgataa agttcgccaa cttcgcttgg aaaacaagat
    17101 tgttccagtt tataagatgg tagacacttg tgcggcagag ttcgattccg aaacgccata
    17161 tttctattca acctatggtt gggaaaatga atctatcaaa tctgataaag aatctgtact
    17221 tgtcctaggt tcaggtccaa tccgtatcgg tcaaggggtt gagtttgact acgcaaccgt
    17281 tcactctgtt aaggctatcc aggcagctgg ttatgaagct atcatcatga actcaaaccc
    17341 agagaccgtt tcaacagact tctctgtatc agataagctt tactttgagc cattgacatt
    17401 cgaagatgtt atgaacgtca ttgacttgga acaaccaaag ggagttatcg ttcagttcgg
    17461 tggtcaaaca gccatcaacc ttgcagagcc attggcaaaa gcaggtgtga ccatccttgg
    17521 tacacaggtt gctgacctag accgtgccga agaccgtgac ctcttcgagc aagctctcaa
    17581 agaattggat attccacagc caccaggaca aacggctacc aatgaagaag aagcagctct
    17641 tgcagctcgt aagattggct tcccagttct tgtccgccca tcttatgtac ttggtggacg
    17701 tgccatggaa atcgttgaaa acgaagaaga ccttcgttct tacatgcgaa ctgctgttaa
    17761 ggctagtcca gaccatccag ttcttgtcga ctcttatatc gttgggcaag agtgcgaagt
    17821 tgatgccatt tcagatggta aaaatgtact cattcctggt atcatggagc atatcgaacg
    17881 tgctggtgtc cactcaggtg actcaatggc tgtttaccca ccacaaacct tgtcgcaaaa
    17941 ggttcaggag accatcgcag actacaccaa acgcttagca atcggtcttc actgccttgg
    18001 aatgatgaac atccagtttg ttatcaagga tgaaaaagtc tacgttattg aggtcaatcc
    18061 acgtgccagc cgtaccgttc cattcctatc taaggtaacc aatattccta tggctcaagt
    18121 agcgaccaag ctcattcttg gtcaaagttt atcagaactt ggctaccaaa atggacttta
    18181 ccctgaaagc acccgtgttc atatcaaggc acctgtcttc tcctttacca aactagctaa
    18241 ggtagacagc ttgctcggtc ctgaaatgaa gtcaacaggt gaagttatgg gttctgatgc
    18301 tactttggaa aaagctctct ataaagcctt tgaagcttct tatctacact tgccaacttt
    18361 tggtaatgtc gtctttacca tcgctgatga tgccaaagaa gaagccttga acttggctcg
    18421 tcgtttccaa aacattggtt atggaatcct tgcgacagaa gggacagcag ccttctttgc
    18481 tagtcatggc ttgcaagccc aacctgttgg taagattggt gatgacgata aggatatccc
    18541 aagttttgta cgcaaaggaa gaattcaagc tatcattaac acagtaggaa caaaacgaac
    18601 tgctgacgaa gatggtgagc aaattcgtcg ttcagccatt gaacacgggg tgcccctctt
    18661 cacagcgcta gatacagcta atgccatgct taaggtactt gaaagtcgta gctttgttac
    18721 agaagcgatt taaatagaaa aataagatat agcaga
//
LOCUS       DAVMEF010000024        18618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMEF010000024 DAVMEF010000000
VERSION     DAVMEF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.024"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(66..719)
                     /gene="phoU"
                     /locus_tag="VYQ30_000592"
     CDS             complement(66..719)
                     /gene="phoU"
                     /locus_tag="VYQ30_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1965754.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(731..1489)
                     /gene="pstB"
                     /locus_tag="VYQ30_000593"
     CDS             complement(731..1489)
                     /gene="pstB"
                     /locus_tag="VYQ30_000593"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1965755.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(1502..2305)
                     /gene="pstB"
                     /locus_tag="VYQ30_000594"
     CDS             complement(1502..2305)
                     /gene="pstB"
                     /locus_tag="VYQ30_000594"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1965756.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2316..3200)
                     /gene="pstA"
                     /locus_tag="VYQ30_000595"
     CDS             complement(2316..3200)
                     /gene="pstA"
                     /locus_tag="VYQ30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1965757.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3190..4107)
                     /gene="pstC"
                     /locus_tag="VYQ30_000596"
     CDS             complement(3190..4107)
                     /gene="pstC"
                     /locus_tag="VYQ30_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1965758.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4107..4985)
                     /gene="pstS"
                     /locus_tag="VYQ30_000597"
     CDS             complement(4107..4985)
                     /gene="pstS"
                     /locus_tag="VYQ30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1965759.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIANKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMKGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5559..6863)
                     /locus_tag="VYQ30_000598"
     CDS             complement(5559..6863)
                     /locus_tag="VYQ30_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1965760.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6864..7637)
                     /locus_tag="VYQ30_000599"
     CDS             complement(6864..7637)
                     /locus_tag="VYQ30_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1965761.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNGRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7627..7905)
                     /locus_tag="VYQ30_000600"
     CDS             complement(7627..7905)
                     /locus_tag="VYQ30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1965762.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7905..8306)
                     /locus_tag="VYQ30_000601"
     CDS             complement(7905..8306)
                     /locus_tag="VYQ30_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1965763.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8516..9133)
                     /locus_tag="VYQ30_000602"
     CDS             complement(8516..9133)
                     /locus_tag="VYQ30_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1965764.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(9123..9896)
                     /locus_tag="VYQ30_000603"
     CDS             complement(9123..9896)
                     /locus_tag="VYQ30_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1965765.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(9907..10644)
                     /locus_tag="VYQ30_000604"
     CDS             complement(9907..10644)
                     /locus_tag="VYQ30_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1965766.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(10649..11779)
                     /locus_tag="VYQ30_000605"
     CDS             complement(10649..11779)
                     /locus_tag="VYQ30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1965767.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11863..12252)
                     /locus_tag="VYQ30_000606"
     CDS             complement(11863..12252)
                     /locus_tag="VYQ30_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1965768.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQVKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12268..12651)
                     /locus_tag="VYQ30_000607"
     CDS             complement(12268..12651)
                     /locus_tag="VYQ30_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1965769.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12652..13440)
                     /locus_tag="VYQ30_000608"
     CDS             complement(12652..13440)
                     /locus_tag="VYQ30_000608"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1965770.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13433..14368)
                     /locus_tag="VYQ30_000609"
     CDS             complement(13433..14368)
                     /locus_tag="VYQ30_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1965771.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14498..14674)
                     /locus_tag="VYQ30_000610"
     CDS             complement(14498..14674)
                     /locus_tag="VYQ30_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1965772.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14815..15522)
                     /locus_tag="VYQ30_000611"
     CDS             complement(14815..15522)
                     /locus_tag="VYQ30_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1965773.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            15673..16701
                     /gene="queA"
                     /locus_tag="VYQ30_000612"
     CDS             15673..16701
                     /gene="queA"
                     /locus_tag="VYQ30_000612"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1965774.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(17370..17759)
                     /locus_tag="VYQ30_000613"
     CDS             complement(17370..17759)
                     /locus_tag="VYQ30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1965775.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(17787..17912)
                     /locus_tag="VYQ30_000614"
                     /pseudo
     CDS             complement(17787..17912)
                     /locus_tag="VYQ30_000614"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17855..18496)
                     /locus_tag="VYQ30_000615"
     CDS             complement(17855..18496)
                     /locus_tag="VYQ30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002871528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1965776.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDVDNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKTLKQELLKKEPSPKFLSKKFQSKQNISMTQLLIKVKK"
BASE COUNT         5497 a         4301 c         3413 g         5407 t
ORIGIN      
        1 ttttcgttca aaaaaaggag ccatcagttg atttcaagct cctttttata cagaattaaa
       61 ctattttata gttcgacaat cttacctgtt tcaaagtaga caacccattc acagatattt
      121 ttagcatagt caccgatacg ctccaagtag gaaataactt ggaaataatc acgacccgta
      181 acaatggctt ctggattttt cttaatctct tcagtcgcaa ggtcacggat agtttcaaaa
      241 tagtggttaa tttgctcatc catggaggcc acccggtatg cgtcgtcaac agaaccatta
      301 agataaagat caagtgctgc ttcaacaacg cttttaactt cacgtcccat ttttttaatt
      361 tcttcctcta cagctggaat gcgctcttcc cccttcatac ggatggttgc ctgagcaatg
      421 gctacagcgt gatcccccat acgctccaca tctgatacag ccttaaggac agtcaagact
      481 gtacgcaaat cttgagagac tggttgttgg agtgcgatca tttcaaatga tttcttttcc
      541 agtttcactt cgtattcatt tacttctgca tcatcttcga tgacctcttt tgccaggtca
      601 cggtcatgcg tgacaaaagc acgtaccgta cgattgattt gtgagagcac ttcttgtccc
      661 atagcgtaga actggttatg taatttctct aaatcttctt caaattgaga tcgtaacatc
      721 tttcatctcc ttatccaaat tttcctgtaa tatagtcttc cgtttccttg tgttggggat
      781 taaggaacat ctgcttggta tcattaaatt caatcaaatc tccatctagg aaaaatcctg
      841 tcttatcaga gatacgtgaa gcttgctgca tggaacgggt taccagaagc atggtgtact
      901 tgtcttttag accatacaaa gtttcctcaa ttttaccagc tgaaatcgga tccaaagccg
      961 aagttggctc atccaagagg atgattttag gactagttgc caagacacgg gccacgcaga
     1021 cacgctgctg ttgaccacct gacaatccaa tagctgaatc atatagacga tccttgacct
     1081 catcccagat agaggcacct tgcaaggctt tttctacggc ttcatccaga acctgcttat
     1141 ccttaattcc attgatacga agcccgtaga caacattctc atagatagtc atagggaaag
     1201 gattaggttg ttggaaaacc attccgattt ccttacgtaa ttcaaccgta tctgtacgcg
     1261 gactgtagat gttgtgacca ttgtacacca cggatccagt tgtggtcacc tctggattga
     1321 gatctcccat gcggttgaga gacttgagga gggttgactt ccctgatcca gatggaccaa
     1381 tcaaggctgt aatttcctta ggttggaaag atagggaaac actattcaaa gccttctttt
     1441 tattataata aacggacagg tctgatacct gtaaaatcgc atctgtcata cggtttcctt
     1501 tctaaccaaa gtgaccagat acatagtcat tggtggactg tagcttggca ttttggaaaa
     1561 tagttgcagt cttgtcatac tcaatcaaat cacccaagta aaagaagcct gtatagtcac
     1621 ttgcacgagc agcctgctgc atattatgcg ttacaatgat gatggtaaag tttttcttga
     1681 gctcaaacat ggtctcttct agttgcatgg tcgcaatcgg atccaaggct gaggctggct
     1741 catccattaa gaggatatct ggcttaacag agatggcacg agcgatacag agacgttgtt
     1801 gctgaccacc tgataaggtc aaggctgact tgtggagatc gtctttaacc tgatcccaga
     1861 gggcagcctg acgaagggag gtttctacga tttcatctag gacttgctta tccttaactc
     1921 cagcacgttc atgcgcaaag gtaatattac ggtaaattga cttagcaaat ggattggggc
     1981 gttgaaaaac cattccaatg tgtttacgca tttcataaac gttgatttct ggacggttga
     2041 catcaattcc acgatagaga atctgcccag ttactttagc aatatcaata gtatcattca
     2101 tgcgattgag actgcgtaag taggtagatt tccccgatcc cgacgggcca atcaaagctg
     2161 taattttatt tctttcaaat tgcatatcaa tccccttaat ggattcattt ttaccatagt
     2221 aaacatggac atccttagta gaaagggcta ctttttcttc aggaaaggta aggatatgct
     2281 tctcatccca gttatatgtt gacatggctt ctcctttagg cagcggttaa tttcttgtgt
     2341 agatagcttc cgaacttacg agctccaaag ttaaaaatca ggataaagat caggagcaca
     2401 gcggcagaac ctgctgatac aattgttcca tctggaatag tgccttcact attgactttc
     2461 cagatatgga cagccaaggt ttctgcttga cggaagatag agatggggct agtcacactg
     2521 aggatattcc agttagacca gtcaagagct ggcgccgatt gccctgctgt atagatcaga
     2581 gctgcagctt cgccaaagat acgaccagat gccaagacga cacccgttac aatacctgga
     2641 agcgcttctg gaataacaac atgaaccact gtctcccagc gagaaatccc aagagccaga
     2701 ccagcctcac gttgggtatg gtgaacgtgt ttcaaactat cttctacatt acgcgtcatc
     2761 tgaggcaagt taaagactgt caaggccaag gcacctgaaa tgattgaaaa tccatactca
     2821 aactggacta caaagatcaa gtaaccaaag agacccacca ccactgatgg taaagaggac
     2881 aagatttcaa tacaagtccg cacaaagttg gtaacaggac cttttttagc atattcagcc
     2941 aagtaaatcc cagctcccat agaaagaggt acagaaataa tcaaggtaat gaccaatagg
     3001 aaaaaggaat tgtaaagctg aatgccaatc ccaccacctg cttgaaaagc agaagacctt
     3061 ccagtcaaga aagaccaaga gatatggggc aagccccgaa ccaagatata gagaatcaag
     3121 gaagccaaga ttgtcacaat gatgctagca atcgtataga ggacagctgt tgcaagttta
     3181 tctaatttct tagcgcgcat aatttttctt ccctctttct ttcgtaatca atttaatcac
     3241 actgttaaaa actaaactca tcaagagcag taccaaggcc agtgaccaga gaacattatt
     3301 atttacagtt cccatgacag tgttcccaat tcccatagtt aatatagaag ttaaagttgc
     3361 agctggtgtg gtcaaggaag ttgggataac agctgagttt ccgacaacca tctggatagc
     3421 taaagcctca ccaaaggcac gcgccatccc aaagaccact gcagtgaaaa taccagaacg
     3481 ggccgccttc aagattacac gccagatagt ctgccagcga gtggctccca tagcgaaact
     3541 ggcctcacga taataacgag gaaccgcacg caagctatcc gttgtcataa aggttacggt
     3601 cggcaaaatc atgacaaaga ggacggaaat ccctgacaaa atcccaaaac cagtcccacc
     3661 aaagacactg cgaacaaagg gaacgacgac ttgcaagcca ataaatccgt acactactga
     3721 aggaatccca accaggagtt caatagctgg ttgtaaaatc ttcgcccctt ttggtgatac
     3781 ttcggtcata aaaactgctg caccaatagc aaagggtgtt gcgataaggg ctgagagaat
     3841 ggtaacgata aaggaaccca aaatcatagg aagggcacca aattctttac tagaaggatt
     3901 ccaagttcct cccaaaagaa agtcaaagat attcacacca ttgacaaaga aggtcgacaa
     3961 gcctttttgc gctacgaaaa ccaaaatcat ggccacaagg atgactatca aagaaagaca
     4021 ggcaaaggtc aaaccttttc ctaatttctc cagacgagaa ttctttgatg gaagcaacat
     4081 tttcttagct aattcttctt gattcattat tgtctccctt ccaacactgt cacagttccg
     4141 gcagcatctt tttcaacctt catttcctta atcggaatat acttcaatcc tttgacaatc
     4201 ccttcttggg tctcatccga gagaacaaaa ttgagaaatt ctgcagccaa ctcattgggc
     4261 tgccccaatg tatacatatg ctcataagac cacaagggcc aattattgct acttatattt
     4321 tctggactta agtcatagcc attcaacttc atgcttttga ccgaatcatc tatataggta
     4381 agagataaat aagagatagc tcctggactt tttgatacga ttgattttac cgctccattt
     4441 gaatcctgct cctgactttg catggcagac tgacctttca taatgacagt atcaaaggta
     4501 gcacgagagc cagagccggc tgcccgattg ataacagaga tgggtaagtc cttaccacca
     4561 acctctttcc aattggttac ctcacctatg aagatttgac gaagttgctc tgtcgttagg
     4621 ttatcaacat cgacctcctt attggcaatc agagccaagc cagctaccgc gaccttgtga
     4681 tcaacaagag cagaagcatc aattccgtct ttttcttcag caaatacatc tgagtttcct
     4741 atatcaactg ccccagactg aacctgggac aagcctgtac cagaacctcc cccttggaca
     4801 ttgaccgttt ttccaacatg gatcgtgcca aattcatctg ccgctacttc aaccaagggt
     4861 tgcaaagcag ttgagccaac agccgttatg gattctccac gatcaatcca gctagcacag
     4921 cctactaaac aagccgtcag ccaaaaagcg ataagagaca gagcaagctt ttttcttttt
     4981 ttcactgttt ttctcctcga aaataattat gaatactgtg aattttttaa gtagttcttt
     5041 atgagttgac gcatgaattc ttaccaaatt tctgcgcaat tgattattta tataatatag
     5101 gctatattac tctttcctaa cctccttttt tcatatgtgg ataaaatctc ttgtctatcc
     5161 cttcccccat tgtcacccat tatagtcatt tcgtgtctct ttttcccctt tttaatgcaa
     5221 gggaaattac tcttcttaga tgataatcca aaagctagaa aggtatctca aacctctcta
     5281 ctctcccaga ctagtttaca actaaaagga aaagattcta ttttatgaga aatctagttt
     5341 acaagcggta agaacgctaa taactaaact tcttgtactc tttgaaaatc tcttcaaagc
     5401 agtgttttga gctatctatg gctagcttcc tagtttgctc tttgattttc atcgagtagt
     5461 aaaactacat gtgatggcaa tcaagatatc aagaatcatc ctactaaaaa aatccatact
     5521 ttcactataa catagaataa gatatttgac tagcattttc atttgaatct gaggcctttt
     5581 ggaaaataat ttttcaaaac atttccagta acctttgcaa agcccaagcc attgccttta
     5641 accaaaactt ggtaccaacc atttggcaga ctttctgcca gctgaacggt ttctccagcc
     5701 gcatacttga caaacgcttc ttggccaatt tcaaccgact gttcgacctg actcggtttc
     5761 aaggctaaac caagagcgaa actgggctca aagcgtttct tcttaaaagt acccagatgc
     5821 agtccattgc gagcaatctt gagcttccct aaatctggca aaagttctgg caagagataa
     5881 agctggtctc caaaaatctg caagataccc ggtagattga ccttcaaatg gttttgggca
     5941 aattcctgcc acaaggcaac ttgttcacgg ctgaggttac tcttacttgc cttaaattta
     6001 ggagctggat tgttaccctt aaactgtaga tgggcaacaa actgaccctc tcccttaaac
     6061 tgatgagggt acatccgagc cgtttctggc aggtcaatac cagctaccat tccattgata
     6121 tgctctactg gcaacaagtc aaaatcatac tcttccagca accaattgac aatctcttcg
     6181 ttttcctcgg gtgcccaggt acaggtcgaa taaaccagac gaccaccttc agctaacatg
     6241 gtcactgcat cctccagaat ttctctttgc aagctagcac attgactcgg ataatctaag
     6301 ctccaatagt ccatagcatc aggttgctta cgaaacattc cttcaccaga gcaaggggca
     6361 tcaagaacga ttaagtcaaa atagccttta aagaccttga ccaagcggtc ggcagattca
     6421 ttggtcacca cgacatttgt cgctccaaaa cgctccatgt tttcaaccaa aatcttagcc
     6481 cgtttgcttg aaatttcatt ggaaacaagt agcccctccc ctgctagata ggctgccagt
     6541 tgagttgatt tgccccccgg tgcagcagcc aagtccaaga ccttcatacc aggactgggt
     6601 tgggctactt gagccaccat ttgagcagca ggttcttgcg aataaactaa acctgtagca
     6661 tgctcaggcg atttccctga aaccttccca tagtggcccc aaggggtttg agtaatggca
     6721 tcagaaaagg aaagttgctc ttcttttaag ggattgaccc gaaaggccga aaccgcttcc
     6781 tcctcaaaag aggcaagaaa atctcttgcc tcatctccta gtatctcttt atatttttca
     6841 acaaatcctt ctggaaattg catttaagtt cttttccttt cgtaaatata ggactgaatt
     6901 tcctcctgca tctcaagagg caccatcatg accggctgtc tggtttgaaa atcaggagct
     6961 tcaccaaaaa gggtcacaac ccgatagccc agactttccc ctaaaatact agctgcagca
     7021 taatcccatg gttgcagata agtgagatag gtcaacaaac gccctgacaa aatcttggca
     7081 aaactaatgg ccgcacttcc atagacacga acaccaagaa ccgatcggct caaatcagcc
     7141 agcccccatt cattggtttc cagcatacca ctattccctg caatgagaaa atctccaagt
     7201 ggtttagttt taaaaggagc taggggccta ccatttagac aaactggaaa ttccccacca
     7261 ccgtggtaac aatccccttt gaccacatca taaatcagac caaactgtcc ctgaccattt
     7321 tcaaaataag ccatcataac agcaaaatct tcctgctggg ctacaaaatt attggtacca
     7381 tcaatgggat caatgaccca aaccttgccc tcttgaaccg aggctcgcag acaaccttct
     7441 tcagcacaaa tcttatcctc aggataacgg gacaaaatct caccaaccaa gagttcctga
     7501 acttctttgt ccagtctggt caccaaatct gttggagagg acttggtttc aacacgcaag
     7561 tcttcctgca tatggtcaag aatgtactga cctgctttct taacaagctc tttagcaaat
     7621 tcaaatttac tttccaagag aaatctttcc ttcccctttt tctttggcag cctgaactgc
     7681 tcggtaaagt gaatagccac taaccgtttc aaattctcgt cccaaacgtt tctcttcgct
     7741 cttgcttggc acgaccgctt tgaatccctt ataggagtcc agtaactttt tagcctctac
     7801 tttgccttca taggcagctt caacatcatt aaaaaaagaa agcactgaag caagttcttc
     7861 agtgctccac gacaaatcta gtgggtaact atactgtttg ttcattaact aataccagct
     7921 ctcattcttg cttcttttag ttcttgctta cgataactac gagggagaaa agcacgaatc
     7981 tcatcttcat taaaaccgat ttgcatacgc ttggcatcaa taataattgg acgacgcaaa
     8041 agactaggat actgctcaat caaatgaagc aattccgata ccgaaatact ctctacatca
     8101 atattcaatt tttgaaaaat ttttgaacga gttgaaatga tgtcatcagt accattttcg
     8161 gtcaaggaaa ggatgtgttg caattctttt cttgttaaag gactggtcat aatattgtgt
     8221 tccacaaagg gaaccttatg tttttctaac caagccttag ccttacgaca tgatgtacag
     8281 ctcggtgata gaaatagtgt aatcatgctt ttctcttctt atctatactt tgctacttct
     8341 attatacaaa aaaataaggc gcttgactag ggatttttag aaaaaaagcc tattttttca
     8401 agaaaaatag gctgtttgcg aacgattgac acaattggat ttggttaatt cactcttaac
     8461 gatggtttta aacgatatat atttttatat atgcaaatta aaaacatctt tcatttcact
     8521 tcctacgact tttcagacac agatacccaa agaagctttc atagagggca aaaaagagga
     8581 ggaaggcatg aagaaagaag gtctctggca aaatcataat aacaggatcc ttggctggat
     8641 caaaaagcca ggtatcatct cccacaaaga gaatttgatg gaaaagagta aagaattggt
     8701 caaaaccaat caaaactccc ccaagtccaa tcatcacagg taagactact agagtcagga
     8761 gactttttcg ataaagagac aaaaagtcct ttttcacaat cctattgaca aagacataga
     8821 aacttggcag tgtcactaga gctactagct gaaccaaatg aaagagattc ttgaccactg
     8881 cgaaatggtg cagaccagct gctgacgaac gaaaatcagg catctgtaag acctgactaa
     8941 aaggattggt cagataattc atcaagatat gaaaattgta ttgaatggtt tctggtttta
     9001 gatagactcg attcgttaag tttagccact gaatctccat aggatagaaa atccaagcca
     9061 gataaatggt cagaaggatt gagagggaga ggagaaagag catagagccc caaaagatca
     9121 atttagtttt catcaaaatc ccactccgca aggctagaaa ccacatgtgt cggtgcgatt
     9181 ggcaggccag ctacttcttc tgccttagta aaacctgtcg tcaccaagag cgttggaatg
     9241 ccattgtcaa tcccagcccg gatatcagtc aaataattgt ccccaaccat gattaactct
     9301 tcacgttcca aacctaagtg ctcaaccgcc ttgtccataa tgatggcatt tggttttccg
     9361 atataaaccg gcttcactcg tgtcgctact tcaagcagcg taatcagtga gccagcacct
     9421 ggcaaaagac cgcgttccgt cgggatgttg aggtcaggat tggttccgat aaaatgggca
     9481 cccttttgaa tagcaagagt tgctgtggca aatttttcat agtcgacttg ccaatccaga
     9541 ccaactacca cgtaggcagg gttttccttg tcttccacat aaccagccgc cttgatggct
     9601 tccttgagtc ctgcttctcc gacgacatag acggtctttt caagccccaa atcattcata
     9661 tagtcgatgg ttgccaaagt cgctgtgtag acagtcgata ggggcgtatc gatattaaaa
     9721 ttctgagcca acatctcctt aacactctct ggagtgcggg ttgtattgtt ggttacaaag
     9781 agatagggaa tgtcccgctt ttgcaattca tgaacaaaag tctctccagc agggattcgg
     9841 tctttcccct tataaatggt tccgtctaaa tcaattaaat agcctttata tttcatctat
     9901 ttctccctaa gcctttttta tttcttgcca agtaatgatt gcttgggcat tgataacccc
     9961 atcacttgta atttcatgct tgctttccag tccagtccgt tcaacagccg atgtaatcac
    10021 cccacctggt cgaacttcct tgacatactt gaggttgatt ttcttgggaa tatagtgggt
    10081 caaaaaatcc gctcccatga cctcaaaaat ccagtccaag tatttactgt tattgacatg
    10141 accattcata tccaagtcgt aaaaacgaac atggtaatcc ttgctgatcg gctcttccaa
    10201 ggattcatac ttcggtccac ggataagttt tttatcaaaa tcagactggt aaggagccac
    10261 aatctcaggt tcaacaacat ggacttttcg actgtcgcgg tccatgagaa caaaggtcgc
    10321 catcatgtgg atgagctcct gccccgcttc attataaata gtaaagcgac ggtagcaaaa
    10381 aagtcgattg tagctcaagg cttccgtttc gatggtaatt tcttccgcaa aacgaggcaa
    10441 acgaaccacc tcaatatcat attctgcgat aatccagacc agattatagt cttccaaaat
    10501 ggccttatca ctaactccca gttcaatcga ctgcatccct gaaacttgca gtgacagcaa
    10561 aatcacatct ggaagtttga tatgaccgtt catatcagcc atatcaaaag gaattttcat
    10621 tttcatttga taagttaagc ccatgatcct actccaaaat aaatcgttct gctacagtat
    10681 ctcccaaaaa gagacctctc tttgtcatgc gaacgtggtc accctcaatc tgcatgaggc
    10741 cttgttgaac caaatctctg acaatttctc cataaagtcc agcaaaagac tgtccaaatt
    10801 tttcctcaaa tcgcgccatg gaaaccccgg atttcttgcg gagtcccaag aacatttctt
    10861 cttccatttg ctccttttga ctcaggtgat cttctgtaat acaagcattg ccttcctcaa
    10921 ccgcactaag ataatgacga atgggaccat gatttttata acgtactcca ttgacataac
    10981 cagatgcccc tgcaccaata ccatagtatt cagcattgtc ccagtacatg agattatgac
    11041 gactttcaaa gccgggtttg gagaaattag aaatctcata atgctcaaaa cccgctcgct
    11101 ccagctctgc aatgatgtac tcaaacatct ccgcttctag ttcctcctta ggcagaggca
    11161 atttcccacg tcgcatccgg ttcataaaga ccgtatggtt ttctaaaatc aaactataca
    11221 aactcatgtg gggaatatcc agtccaatgg ctttagccac attttccttt acttgctcca
    11281 tggtctgacc aggcagagca taaatcaaat caatggagat gttgtcaaaa ccagccagtt
    11341 tcaggcgatc gatattttca taaatatcct tctccaaatg actgcgccca atctttttca
    11401 acatcttatc atcaaaggtc tggacaccta gcgaaacacg attgacagcc gaatttttca
    11461 aaacagctat cttatccgca tccaaatcgc ctggattggc ttcaatggtc aactcttcta
    11521 agacagacaa gtccaaattt ttagtcaagc cattcagtaa aacctccagt tgcggagccg
    11581 acagggctgt cggtgttccg ccaccgatat aaagggttga caacttttca atatcataag
    11641 aacgaaactc ttccagcaga tgctctaaat agctgtcgac tggctgattt ttgatgaaga
    11701 cctttgaaaa atcacaataa taacaaatct gggtacaaaa tgggatgtgc acataggctg
    11761 acgttggttt tttctgcata gtaattatta taccacaaag actagattcc agataaaaat
    11821 caccatcccc agatacatag tccgtccgga gatggtgatg gtttattctt ctgttatatc
    11881 aatcacaatc tcttctgagt catcaagagc ttcggctttt tcttgccatt gttccttgag
    11941 attatttaat tgattttttg atgcttctgt cgcttgaaaa gcataggatt taacttgagc
    12001 aagtatactg tccacagtga tttcacctga ctcaacctgt tcttttgttt tcagaacaaa
    12061 atctgtagcc tgctccttaa cttctgtcag tttttcacag acttgctcct tggcatactc
    12121 cggatcttct ctcaaatcat ctagaaaatc ttgagcctga ctgcaaactt gtttgccctt
    12181 atcacttgtt aaaaacaagg caagagctgc acctgaaacc gttcctaaaa ggattgagga
    12241 taatttaccc ataaggattc tcctttttta ttttttgaaa aatttacttg caagacgaag
    12301 agctgacaga cttgcaccag tcttgagtgt ttttgaacca gctgatgaag ctttcttgct
    12361 caagacacgc gcatggtcat tgaggtctga aacagataga gataaatctg caacagcact
    12421 gaagagtgga tcaatcgtag ccaccttgac attgatatca tctgccaaga cattgacctt
    12481 agccaacaac tcattggtgt gatgcaaggt cacatccaca tctgaagtca aggttttaat
    12541 cgtcttttct gtttcatcga tgacacgacc aagcttttgt acagtaatga tcagatagac
    12601 caaaaagaca atcaaagcta gggcaacaag aatatatgca acttctaaca tttagttttc
    12661 ctcctctgta atatagtaag gggccttctt tcgattttga taaataacga tcattatacc
    12721 gagaccgata aggacaactg acagccattg ggacactcga agtccgaaga acatgagact
    12781 atctgttcgc ataccttcga tgaccatacg accgaaacca taccaaatca agtaaaaggc
    12841 cgtgatatga cctcgtctga gactcttcca tttccgtcta aaaatcagaa tcaaggcaaa
    12901 gccaagcaga ttccatagag actcataaag gaaagtcggt tgacggtagc tcccctcaat
    12961 atacatctgg tcacggataa agccaggtag ataatccaga ttatccactg ttgcaccata
    13021 agcttcttgg ttaaagaaat taccccaacg ccccaaactt tgagcaatca taacgctagg
    13081 cgccgcaata tctagaaagt cccaagtatt gatgagttta cggtcagcaa agatatagag
    13141 cacaagagcc ccagttatca aaccaccgta aatggccaaa ccaccattcc aaatggcaaa
    13201 aatctctcct aaattctgac tatagtaatc aaatcggaaa ataacatagt agagacgagc
    13261 tcctaaaata gccaagggaa aggctactaa gataaaatct aaaatatcgt ctggtatgat
    13321 cttctttcta ggtgcttctt tcatggtcaa ataaaccgca agaatcaagc ctgtcacaat
    13381 acataaggca taccaacgaa tggctagggg tcctagttga atagcaattg gatcaagcat
    13441 tttgcacctc atttcgagcg atgagacttg tcagtcgttc gtcgaacaaa cgggtcgcat
    13501 caaagcccat ttccttggca cgataattca tggcagctgc ctcaatcaca acagagatat
    13561 tacgacctgt tttaactgga atacgaatac gaggaatggc tacgccagaa acttcaagtt
    13621 cctctgcatt gtttccaaga cgatcaaagg tcttatgcgt atcgtaattt tccaaataga
    13681 cagcaagctg aacctgtgaa gaatccttga cagcactcgc accgtagaga ctcataacat
    13741 cgataatacc aaccccacga atttcaatca agtgtttcaa aatttcagct ggttcacccc
    13801 agagagtaat ctcatccttg gcaaagatat cgacacggtc atcggctacc aaacggtgac
    13861 cacgtttgac aagctcaaga cctgtctcgc tcttaccaat tccactatct ccctgaatca
    13921 agacgcccat cccataaata tccatcaaga caccgtgcac actggtacgt tctgccaaac
    13981 gagaatccag atagctagat aactctccag ataaacgact ggtagctgca cggctggtta
    14041 aaatagcaat cttacattct ctagcagcct ttaacatctc ctctggaacc accaaaccac
    14101 gggcaacaat gactgctggt gtctcaggta gaaacatttt tttcaaaact tcataacggc
    14161 tgttggaagg catgctgatc agataagacc actccttcat ccccaaaagt tgaatccgct
    14221 ctggtgtata gtagtcaaaa tagcctgtca tttcaagacc aggtcgcgta atatccgctg
    14281 tattgatttc cttttcaagc aattctggtt caccatagac aatgtctagt ctaagctttt
    14341 caatcacttc ttttactaaa accgacatca tttcctcctt caaatgagtt cttctctcta
    14401 ttatatcaaa ttgtaggagg aagtttcaga tttttgcagg ctttggagta tttatttaaa
    14461 agaaaaagga ctagatttct ctagcctttc atttcaatta gaatttttta cgtttacgag
    14521 ctacttctga tttacgttta cgttttacag aaggtttttc atagaattca cgtttgcgtg
    14581 tttcttgaag agtaccagct ttagtaaccg cacgtttgaa acggcgaagt gcgtcgtcaa
    14641 gagattcatt cttacgtact actgttttag acattttttt cactcccttc aagttcaata
    14701 tctatttcat tctacacgta aaaggaataa ttgtcaagca aaaatgcggt ttaattttga
    14761 tttttttctt ccatttatac aagagttgaa agcgttcaat agaacattgc ttttttattt
    14821 ttcgagtaag ctaagcgctt cagcatctgc gatgatggtt acatcagggt gattttggag
    14881 gctacttgca ggtaggttct cagtcactgg gccagatact gttccggcaa tggcttctgc
    14941 tttcgactca ccgtaagcaa aaagaataat agacttggca tccaaaatgt ttttaatccc
    15001 cattgaaata gcttgggttg ggacgtcttc aatcttagca aagaagcgtg cattagcttc
    15061 gatagtagac tggtcaagtt ctactagatg cgtttgactg tcaaatggag taccaggctc
    15121 attaaagccg atatgcccat tgcgaccgat tcccaagatt tggaggtcaa ctggatggtc
    15181 agccaaaatt tggttgtagc gttcaacttc agcttcagca ttgtccttaa caccacgagg
    15241 caagaaactt tctttaaatg gtttttggtt gaacaagttt tcttgcatga agtaacgata
    15301 agactgtgga ttgtccccat caaggcccac atactcatca aggttgacac tggttagatt
    15361 tgaaaaatca aggtcactct caacaatttc cttgtaaaat tcaagtgggc tacttcctgt
    15421 cgcaagtcct agagtttgag ctccattagc caatttttcc ttcaaaatct caaaagcaac
    15481 ttttccacct tgaacttggt tttcaacttt aataactttc atcttttcat cctccatttc
    15541 tgtctatatt tattatatgg tatagaccaa tttttgtcaa gtgaaaacga tttaatttct
    15601 aaaaaaagag cgtccaaact tgaaacgtgt tataatggat aggattagaa cttagaaaga
    15661 atgaacagat atatgaatac agctgatttt gatttccact tgccagagga attgattgcc
    15721 caaacgcccc ttgaaaaacg tgatgcctcc aaactcctca tcgtcaaccg tgagacagga
    15781 gaaatgcaag ataaacactt ccactctatt attgatatgc tggaacctgg tgatgccctt
    15841 gtcatgaacg acacccgagt tctccctgcc cgcctctatg gtcaaaaagt ggagacagga
    15901 ggtcatgtgg aacttctcct ccttaagaac actagtggag acgagtggga agttctggct
    15961 aaacctgcca aacgcctcaa ggtcggtact cgtatcagct ttggtgatgg ccgcctcagc
    16021 gctgtcgtta cagaagaatt gacccacggg ggacgcattg tccgctttga ataccaagga
    16081 attttcctag aagtcttgga aagtctggga gaaatgcctc tgccacctta tatccacgaa
    16141 aaattagatg accgtgaacg ttatcaaacc gtctacgcca aggaaagcgg ctctgctgca
    16201 gcaccgactg ctggtcttca cttcaccaaa gaactgctgg cagaaatcca agctaagggt
    16261 gttcatctag tctatctgac tctccatgtc ggactcggaa cctttagacc tgtttctgtg
    16321 gataatctgg acgaacacga aatgcactca gagttctatc aactttctga ggaagctgct
    16381 gccacccttc gctctgtcaa aaaaaatggt ggtcgtgtca tcgctgtcgg aaccacttct
    16441 atccgcacct tggaaactat tggttccaag tttgatgggc aaatccaagc agattctggt
    16501 tggaccaata tctttatcaa acctgggtat gagtggaagg tcgtggatgc cttctcaacc
    16561 aacttccatc tgccaaaatc aactctggtc atgttggttt ctgcctttgc aggccgtgaa
    16621 ttagtcttag atgcctacca ccatgccatc caagaacact accgcttctt cagttttggt
    16681 gactccatgt ttatttattg agaaagaatt tctctaaatc ttctaatacc aataaatcgc
    16741 taagatatta tttcaaagaa catctacaat tgaaactcta gctagctgta gaagacgcct
    16801 agtacattga aattaaaatg cttcccccta gcttcgaaaa tattgccata gattgcgttg
    16861 actctccaaa ttgattcatc tatattttat ttcagcttcc tatattttct tcgctgtttg
    16921 taaatcaaaa tgcaagacac atgagtagca ccatatttgt tactcttatc tgtcctctca
    16981 agagactatt atgagttatt tcagaatcat tcactacttt gaccctgact ctccttagtc
    17041 tcaaaatcaa agacttatac tcttcaaaaa tctcttcaaa ccgcgtcaac gtcaccttgg
    17101 attatatatg tgactgactt cgtcagttct atctacaacc tcaaagcagt actttgagca
    17161 acctgcgact agttttctag ttttctagtt tgctctttga ttttcattga gtattaaaca
    17221 aaaagtgaac aaatctgaat tctaatgtac agaagactag gcttgttcac ttttttatag
    17281 tcgctataag atgaccttat ctatagcttt ttatatataa ttatatattc agacatacta
    17341 ttatcaattt tgtctcaggg aggaatctgt taacgcaccc attcaccatt atcattgact
    17401 ctatagccat ctatacttgt attgaccgct aactcacccg atgcatttac ataataccat
    17461 ttaccaccaa cttggaacca ttgattaact ttcatagaac cgttgctgtt gaggtagtac
    17521 catgaaccat taacttgtac ccaacctgtt gccatggaac catcagtatt ataaaaatac
    17581 cacataccat tttcttgttt ccagcctgtt gttggagcaa ctgctttagc tggttctact
    17641 gctacatctg ttccttggtt agatgtaaca gatacaggat acgaaggaat agatgattgc
    17701 tcaggaacaa caactttttc aggttctctc gtccctctcc ttatacgtct ttttaccatc
    17761 tctttagtaa tttgacgaga agtagtttct tcaattgttc catcacgttc atctacagca
    17821 tagattgtag taagagtaat tttaccaatt tctcctactt cttgaccttt atcaagagtt
    17881 gggtcatcga gatattctgt ttcgattgga atttcttgga caagaacttg gggcttggtt
    17941 ccttttttaa caactcttgt ttgagagtct ttttttgact taaagtactc tcagttactt
    18001 gtccactctt tccatctaca ttataagtta tcgttgtaac tgttttccca ttctttccta
    18061 gagtaatctc ttgctcctgt cctgcagaaa ggtcattgtc tgcttcatat ttagtagcaa
    18121 atggaacaag aacttcttca accttgcttt tagctggaac tttgataact gtatccgtgg
    18181 cttcttttct atcaacagta acctgttcgg taacataacc agtctctgga ttaacatcgt
    18241 aggtccttgt cgtagttaca tagccatcct ctccatcaat tgtaacagga ttttcactac
    18301 ggtcttttgt ttcatctttt tcataacgaa ttcgcgtact tgaaattttc ttggttacta
    18361 ccttaggttt agtcgctact tttacaataa tatccccatt gtcaacgtca tcatactcta
    18421 ttccctcttc tttatctcta gtatcatctc tgacatattg aatcccatca gcagcatgaa
    18481 caaaacttgt attcagattc ctcctaaaaa taaagttagc ccgattaccg cagaaccaaa
    18541 aatctttccg agtttacgta ttgcatagcg cttattagta ttagattttg ccattacatc
    18601 ctacttctag tatagcat
//
LOCUS       DAVMEF010000025        18246 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMEF010000025 DAVMEF010000000
VERSION     DAVMEF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.025"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            122..418
                     /locus_tag="VYQ30_000616"
     CDS             122..418
                     /locus_tag="VYQ30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1965777.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYKDVIDSNGASIE"
     gene            539..1483
                     /gene="manA"
                     /locus_tag="VYQ30_000617"
     CDS             539..1483
                     /gene="manA"
                     /locus_tag="VYQ30_000617"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1965778.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1474..2990)
                     /locus_tag="VYQ30_000618"
                     /pseudo
     CDS             complement(1474..2990)
                     /locus_tag="VYQ30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3095..3844
                     /locus_tag="VYQ30_000619"
     CDS             3095..3844
                     /locus_tag="VYQ30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1965779.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTDQYTSDAIAAYVQTNAEKIG"
     gene            complement(3864..4319)
                     /locus_tag="VYQ30_000620"
     CDS             complement(3864..4319)
                     /locus_tag="VYQ30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1965780.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4339..5514)
                     /locus_tag="VYQ30_000621"
     CDS             complement(4339..5514)
                     /locus_tag="VYQ30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1965781.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5573..6418)
                     /locus_tag="VYQ30_000622"
     CDS             complement(5573..6418)
                     /locus_tag="VYQ30_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1965782.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            6543..7100
                     /locus_tag="VYQ30_000623"
     CDS             6543..7100
                     /locus_tag="VYQ30_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1965783.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7119..7586
                     /locus_tag="VYQ30_000624"
     CDS             7119..7586
                     /locus_tag="VYQ30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1965784.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7675..8304
                     /gene="upp"
                     /locus_tag="VYQ30_000625"
     CDS             7675..8304
                     /gene="upp"
                     /locus_tag="VYQ30_000625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1965785.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8478..9068
                     /gene="clpP"
                     /locus_tag="VYQ30_000626"
     CDS             8478..9068
                     /gene="clpP"
                     /locus_tag="VYQ30_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1965786.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SIEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9147..9395
                     /locus_tag="VYQ30_000627"
     CDS             9147..9395
                     /locus_tag="VYQ30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1965787.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9496..10656
                     /locus_tag="VYQ30_000628"
     CDS             9496..10656
                     /locus_tag="VYQ30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1965788.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGRDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10924..11793
                     /locus_tag="VYQ30_000629"
     CDS             10924..11793
                     /locus_tag="VYQ30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1965789.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGAISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFEMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11797..12753
                     /locus_tag="VYQ30_000630"
     CDS             11797..12753
                     /locus_tag="VYQ30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1965790.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12753..13517
                     /locus_tag="VYQ30_000631"
     CDS             12753..13517
                     /locus_tag="VYQ30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965791.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13517..14227
                     /locus_tag="VYQ30_000632"
     CDS             13517..14227
                     /locus_tag="VYQ30_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965792.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14586..15242
                     /locus_tag="VYQ30_000633"
     CDS             14586..15242
                     /locus_tag="VYQ30_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1965793.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15350..16445
                     /gene="prfB"
                     /locus_tag="VYQ30_000634"
     CDS             join(15350..15421,15423..16445)
                     /gene="prfB"
                     /locus_tag="VYQ30_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1965794.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16463..17155
                     /gene="ftsE"
                     /locus_tag="VYQ30_000635"
     CDS             16463..17155
                     /gene="ftsE"
                     /locus_tag="VYQ30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1965795.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17148..18074
                     /locus_tag="VYQ30_000636"
     CDS             17148..18074
                     /locus_tag="VYQ30_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965796.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5623 a         3428 c         3973 g         5222 t
ORIGIN      
        1 acgagagtct tttaaaagtg ttttgatggt ttggatttct tctttagttg atttcatatt
       61 actattgtat aatgcttttt gatttgagtc tagtattatc gtagaaaatg gactcggtgc
      121 tatggataca ccggatgaga atggttatgt agcagatgac tatcggatta cttacttaga
      181 ggcccacatc aaggccatgc gagatgccat ttaccaagat ggggttgact tgcttggtta
      241 tacgacttgg agctgtatcg atccggtttc agctggcaca ggggaaatga ataagcgcta
      301 tggctttatc tatgtagatc gagataatgt aggaaatggg actctcaaac gtagcaagaa
      361 gaagtccttc tattggtaca aggatgtcat tgatagcaat ggtgcaagca tcgagtagag
      421 cacatttgtt tactattttt ataaaatctt ctccctgctt tataagatgt aaaaaagagt
      481 tcaaatggaa ctcttttttt gctataatga ggggagaaaa atcagacagg agattaagat
      541 gtcagaacca ttatttttac aatcagttat gcaagaaaaa atctggggtg gtaccaagct
      601 acgtgatgag tttggctacg acatcccaag tgaaaaaatc ggagaatatt gggccatctc
      661 agcccatcca aatggagtct ctaaagttgc caatggtcgt tacgagggaa cagatcttgc
      721 tactttgtat gcggaacacc gtgaattatt tggcaatcgt ccagaacctg tatttccact
      781 tttgaccaag atcctcgatg ccaacgactg gctcagtgtc caagttcacc cagacgatgc
      841 ttatggactc gagcatgaag gcgaactcgg aaaaacagaa tgctggtaca ttatcgcagc
      901 ggatgaaggt tcagagatta tctacggtca caatgccaag tcaaaagaag aactccgcca
      961 gcaaatcgag gataagaact gggatgcgct gttgacaaaa gtgccagtta aagcaggaga
     1021 tttcttctat gtaccaagcg gtaccatgca cgctatcggg gcgggtatct tgattcttga
     1081 aacccagcag tctagcgata ccacttaccg tgtctatgac tttgaccgta aggatgacaa
     1141 gggcaattta cgtgaacttc accttgaaaa atctatcgat gtcttgaaca ttggtgaacc
     1201 cgcaaatagc cgtccagtga caatcaaagc agatgatcta cgttccactc tccttgtatc
     1261 caatgatttc ttcgcagttt acaaatggga aattactgga aaagttaact ttgaaaagac
     1321 agctgactac agcttgttga gtgtcttggc tggtcaaggt caactaactg ttgacgggaa
     1381 aaactatcca atccaaaaag ggagacactt tatcctacca agtgatgttg aagcttggac
     1441 tctggaaggg caagatttag aattaattgt tagtcatcca taaaaaagaa agggcctgag
     1501 ttcattactc aagtcttttt gattacataa attgggcgat gaagaccacg atgatgattg
     1561 gaatgacgga acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca
     1621 tgctctttca tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt
     1681 aaaaagtgaa aggtagagag ctccaaatgg catgaggaga ttggaaacca agaagtccat
     1741 agcgtcaaag aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc
     1801 tgaaggaatg ccaaagacaa aggtcaaaat tcctaaaata acactccatt tggcacgttt
     1861 gctgttatcc tggttggtga tattgcccac attgatctcc agcatcacga cagaaaaagt
     1921 gactggcgca aaaaggaaga gcaagaggaa aaggacgtag aaaatggttc caaaaggcat
     1981 cttgtcaaag agttgaggca agacgataaa gagcaagctc ggtccccctt cagactggat
     2041 attgaagggg gaccgagctt ggaaaatggc tagacctgcc atgatggata tcgagatatt
     2101 catggctacg atggagattc ctgactggac taggttggtt ttcttgtcca agtaagaagc
     2161 ataggtcaac atgactgtaa cccctagtga gagggcaaag aaagattgtc ccagagcata
     2221 gaggagacca gcgctagtca gttttgaaaa gtctggtttg aggaagtaaa gaaccccttc
     2281 catggcattt ggcaaactga gagagcgacc gatgataaca acaaagatga taaagagcag
     2341 gggcatcatg actttcgaag ctctttcaat ccctttttga accccacgtg atacaataaa
     2401 gatatttaat aagataaagg ctgcttgagc tcctagggca atggctggat ttgaaatgat
     2461 tgaagtaaat aactgagcat aatcacccgt tccaccaagt tggaacaatt tcccaaactc
     2521 aatacctaga tagactagaa tccatcctcc gataacactg taaaaagata agaggataaa
     2581 gagggcaaag gcgccaatcc aaccgataaa gttgtacttg ttattcttgc ccagttttcc
     2641 aaaggttttg atagcggaaa cgccagcact acggccaagg gcaaactcag ccagcaggag
     2701 agggaaaccg attaaaatag tggaaatgag aaagattagt aaaaagcctc catcgccatt
     2761 agcagcagtc atgtagggaa acttccaaac ggaaccaagc ccgatggcca gccagcagat
     2821 gctagaataa aaccaagttt cgacccccat tgtgattttt ctgacatagt taaactctga
     2881 tctccttttt caaaataaga aaaagctact tgagttgtca aatagccttc tgtttgattg
     2941 atagacttga ctatcctatc atgttttcta aggtctgtca agaaaatcat tttcaagttt
     3001 tcacaccttt ctcaaaaaag ttaaaaaatt ttctcaaaaa cgcttgactc tgacctaagg
     3061 ggaggggtta tactatcatt gtaaggagga aatcatgtac catataaaag aagctgcgca
     3121 gctttcaggt gtctctgtca agaccctgca tcactatgac aagataggac tcttggtccc
     3181 cttaaagtcg gaaaacggct atcgaaccta cagtcaagag gatttggaac gccttcaggt
     3241 cattctttac tacaaatatc taggcttttc tttagagaaa atagcagagc tgttaaagga
     3301 agaaaggaca gatttattgc cccatttgac taggcagttg gactatctaa ctcgcgaaag
     3361 gcaacatctg gataccttga tttccacctt gcaaaaaact attcaagaac aaaaaggaga
     3421 aagaaaaatg accattgagg aaaaattcac gggatttagc tatcaagaca atcaaaaata
     3481 ccaccaagaa gcggtagaga aatatggtca agaagtcatg ggacaagcgc tcgaacgcca
     3541 aaaaggtcac gaagacgagg ctacggccgc ctttaaccaa gtctttcaaa ctttggcaca
     3601 aaatcttcaa gttggtttac ctgcaacagc aaccgaaaac caagagcaag cagccaagct
     3661 cttgcaagcc attcgcactt atggatttga ctgctctatt gaggtattcg gtcatatcgg
     3721 taaaggttac gtctataacc cagagtttaa ggaaaacatt gacaagtttg gttctggaac
     3781 agaccagtac acgtcagatg ccattgcagc ttacgttcag acaaatgcag aaaaaatagg
     3841 ctaggaattt cctagcctat tttttacttc aaatcataaa gccagtcgtc accgtttttg
     3901 tagtaaaaga attcactgag atcttcttct agaaacacac gaagcatatc agacatatca
     3961 tcggttgcaa gttttagatg agaaagattt tcaaagtcct cccaccaaac tttcccttcg
     4021 tctgaagact ggagttcacc agtaaagtgt tctgtcttgt aaaaaaggac gacataacga
     4081 taatccttgt cgtcatacca gtttttgata ccacagagtt ggggtttgga aatgatcaga
     4141 ccagtttctt ctttcacttc acgaatgaca gcatcgacaa aggattcgcc acgttcaaca
     4201 tgaccaccag gaaaagtaat gccaggccag tcgggactaa ctcggtcttg gaccaggacc
     4261 ttatctccgt ttttaatcat acacatgtta acaaattcga ctgcctctct tctgttcatt
     4321 cttcacaacc tttaatcttt aatcataatg cagacttccc gccacccagc cggtacagag
     4381 ggcagaagtg atgttaaagc cacccgtgtg ggcattgata tccataactt cgcctgcaaa
     4441 gtggaggcca ggtaccagct tactttcaag ggttttagga ttgatttcct tgagactgac
     4501 tccacccttg gtaacaaagg actttgcaag ggacattttt ccagttacag gaattttaag
     4561 ttctttaatg gactgaacaa gttgttctcg ttccttttca gtcagctgtt tgactttttc
     4621 aggatatcct tgtacaaaaa attcggccaa gcgttctggt aacaaggttt ttaaagcgtt
     4681 tttcaaggat ttttcccgat tttcttctag aaatgtaacc aagtccttct cagaaagttg
     4741 aggcaaaaca tcgagtgaga gaacctcccc acctttgaca aagctagaca tgcgtagggc
     4801 agcaggacct gacaaaccaa agtgggtaaa gagtaaatca tgagtgatga catgcttacc
     4861 ataacttagg gtcacatcgt ccagagaaat cccttgtaag gctttatgtg gaaaatctgt
     4921 taataaagga ctttcagcag cctcaagctc ggtgatggta tgcttaaaat ggcgagcaat
     4981 ctcgtgacca aaaccagtcg aaccagtcga aggataagac ttaccacctg ttgtgacaat
     5041 gagtttctca caagtgaagg tttgatccgc tgacttaagg acaaactggt catctacttt
     5101 tttaacagaa acgatttcta tttgagtagc aacttgacca cctagttcgg tgattttctt
     5161 ttccaaagct tcgataatag tccgagactt gtcactggct ggaaagacgc gtccgtggtc
     5221 ttcgacctta agtttaacac cattttctgt aaaaaagttg atgatgtcat gattatcgaa
     5281 ctgggagaaa acactgtaaa gaaagcgtcc gtttccagga attccagcta gcaggttgtc
     5341 taagctacca ttgttggtca cattgcaacg tcccccacca gtcccagcta atttttttcc
     5401 aagtttccga tttttttcga tgaggagggt tttctgtcca taaaagctac tggaaatcgt
     5461 agccatcata ccagcaggtc ccccaccgat gacaatagta tcaaaatgtt tcatagctct
     5521 attgtaccac aaaaaaacaa gagatgatgg tcacctcttg tcaagaatgc aattaatcaa
     5581 tttcatagcc catcagcaaa ccgccatctt ctgcatagaa actgcagaga ccagaggttg
     5641 gtagaatttt aatatccgct tgtgggaagg tttcacggat tcgctctgag agctgttgac
     5701 aacatttttc gttattgcgt tgggccatga caatacggcc accagcatat ccagctttta
     5761 ctaactcatc ataggcagct tgaactgatt tctttgatcc ccttgctttt tgtagcaatt
     5821 cgagagtccc agtttcacta gcttttccga ccatacgaat gttgagaagg ccaacgaccg
     5881 taccgataag cttgctcaaa cggccgttct tcaccaagtt atcgactttg gctaggacaa
     5941 agagcaactt agttttttct tgataggcgg tgatagcttc aaccacttct tcaaaagaca
     6001 agccctggtc aatcaagtca ttcaattttt ctacgagtag gtcaacttca ccaccagcag
     6061 ataaactatc aatcacatga atcttagtgt caggatggtc ttccagataa atattctttg
     6121 ctagttgagc actattgtga ctgccagaaa gggtacctgt gatggttact aggaaaatgt
     6181 ttttggcacc ttcaaatgct cgcaaatagt catctgggct tggacaagcc gattttgaag
     6241 cttctgcagt tgcatacatg gtttccatca tttggtcaat atcgagactg gcgtcatcaa
     6301 caaagacctg atcagctact tgaatggtta aggggacact tacaaaggtt gtgttaatag
     6361 ctggtgttgg cagttgacga taatcacaac cagagtcagc aataattttc caagtcatag
     6421 aaattctcca tctttgtcag ttatacattg acaaaggttc tgtctttttt tacaattata
     6481 tcatgaaaac ccttgaaaca aaagcatcat ccgtctgttg tgacaagtag aaagaaaatg
     6541 ttatgtctga acgtagaatc tctgaaaagt ctcttgaaaa tctcagaaaa tcaaaccaag
     6601 aatccaattt attaaccaga gaagccattg aaacagccct cttgcaactc ttggaaaaaa
     6661 aggagctgac caagattagt atttctgaat tggtcaaacg tgcaggtgtt tcgcgtgcgg
     6721 ccttttatcg caattatgac tccaaagagg aaattttaga gagcgtcttt aaacgaactg
     6781 tccacaatat tatggaacag ctgcatcatt acgatttaaa gacagacctt tatttggttt
     6841 gggttcacct tttccgggag gccagaaagg aagctagagt gattcaattg gccttggatt
     6901 accatctgga aaaaatcttt gtccaagcca tgcaggaatt tctagaaaaa taccatggga
     6961 aatcaaaagg tgtcagctct tatcttcatt ccttctggag ctcagccatt gtctctgtcc
     7021 ttctaaaatg gatcaaggat ggcatgaagg taccagctga aaagattgca gatttaggtt
     7081 taccattttt taaaaaatag agaaaaagga gaaaagagat gactgaaaaa agactggcct
     7141 gggatgagta ttttgcagcc caagccctac taattgcgaa tcgttccact tgtaaacgtg
     7201 ctaaagtggg cgcgattctg gtaaaagata ataaggttat ttccactggt tacaatggtt
     7261 cggtgtcagg gactgagcat tgtattgatc acgaatgttt ggtcattgaa ggccactgtg
     7321 ttcgcaccct tcacgctgag gtcaatgcta tccttcaagg tgctgagcgt ggtgttccca
     7381 aaggctttac agcctatgta acccattttc cttgtctcaa ctgtacaaaa caattgcttc
     7441 aggtcggctg caagcgcgtg gtttatatca accagtaccg aatggacgac tacgcccaat
     7501 acctttatca agaaaaaggg acagaattga cccatttacc acttgagaca gtacagacag
     7561 ctcttaaaga ggctgatcta atgtaaaaat tatcaaaaat aaatggctta gaaagatttt
     7621 taaaccgttt tttggtataa taagaagaat aaattgaaag aaggaattcc aaaaatggga
     7681 aaaattgaag ttattaatca cccactgatt caacacaaat tgtcaatctt gcgtcgtaca
     7741 gatacttcta caaaagcttt tcgtgagcta gtagatgaga ttgcaatgtt gatggggtat
     7801 gaagtacttc gtgatcttcc actagaagat gtggaaatcg aaacaccaat tacaaaaaca
     7861 gttcaaaaac aattggcagg taagaaattg gccatcgtcc caatcttgcg tgcaggtatc
     7921 gggatggttg atggtctctt gagcttggtt ccagctgcta aagttggcca catcggtatg
     7981 taccgtgatg aagaaacact tcaaccagtt gaatacttgg tgaaattgcc tgaggacatt
     8041 gaccaacgtc aaatttttat agtagaccca atgttggcaa caggtggctc agcaatcttg
     8101 gctgttgatt ctcttaaaaa acgtggcgca tcaaatatca aatttgtctg ccttgtatct
     8161 gctccagagg gtgtaaaagc ccttcaagaa gctcatccag atgtagaaat ctttacagca
     8221 gccttggatg aacgtttgaa cgaacacggt tatatcgttc caggtcttgg agatgctgga
     8281 gaccgcttgt tcggtacaaa ataagatcga aaagaagaat gacttggaaa agtatttcca
     8341 gtcacgaaag gaggttgggt ttttgtttct gtctaatgaa agcagagcaa aaatttgacc
     8401 ttttttgacc aagatattat aatagtctta tcttcagtca tttgaccaac aaaattaaaa
     8461 ctcaaaagga gaaatgaatg attcctgtag ttattgaaca aacaagccgt ggagaacgtt
     8521 cttacgatat ttactcacgt cttctcaaag accgcatcat tatgctgaca ggtccggttg
     8581 aagacaatat ggctaactct gttattgccc aattgctttt cttggatgcc caagatagta
     8641 caaaagatat ttacctttat gtcaatacac caggtggttc tgtttcagct ggtttggcaa
     8701 tcgtagatac catgaacttt atcaaggcag atgtccaaac catcgttatg ggaatggctg
     8761 catctatggg aacagtcatc gcatcaagtg gagcaaaagg caaacgtttc atgcttccaa
     8821 atgctgaata catgattcac caaccaatgg gcggtacagg tggtggtacc caacaaactg
     8881 atatggctat cgctgcagaa cacttgctca aaactcgtaa taccttggaa aaaatcttgg
     8941 ctgaaaattc aggtcagtca attgaaaaag tccatgcaga tgcagaacgt gataactgga
     9001 tgagcgccca ggaaacactt gaatatggct ttattgatga aattatggcc aacaattcat
     9061 tgaactaatg atgatagaag gcaaactcga ctgggtttgc tttttttggt ataataggga
     9121 gagatttctt agaaagagga tttatcatgt ttgaaaaagt caatcgctct ggcttgatta
     9181 tctatcttta ctataatcgt gatgccaaaa aattgcagga ttatggagat attacctatc
     9241 attccaagaa acatcgttac ttacaactct atgttccaac tcaagaagtg gagcaattgg
     9301 tcggacgctt gagcaaggaa aaatttatta aaaaagttag ggtttgtcat atccaagagt
     9361 tggaaacacc ctttgtgggc aatctttatc gataggaaaa cgttatcata gaaaaagttc
     9421 aaggaaaaca ttgacaattt tctgataatt cggtatattc ttaacagact atttaagaaa
     9481 taaggagaca aaaagatgaa gaaaaaattt gccctatcgt ttgtggcgct tgcaagtgta
     9541 gcacttcttg cagcctgtgg agaagtgaag tctggagcag tcaacactgc tggtaactca
     9601 gtagaggaaa agacaattaa aatcgggttt aactttgaag aatcaggttc tttagctgca
     9661 tacggaacag ctgaacaaaa aggtgcccaa ttggctgttg atgaaatcaa tgccgcaggt
     9721 ggtatcgatg gaaaacaaat cgaagtaatc gataaagata ataagtctga aacagctgag
     9781 gctgcttcag ttacaactaa ccttgtaacc caatctaaag tatcagcagt cgtaggacct
     9841 gcgacatctg gtgcgactgc agctgcggta gcgaacgcta caaaagcagg tgttccattg
     9901 atctcaccaa gtgcgactca agatggattg actaaaggtc aagattacct ctttattgga
     9961 actttccaag atagcttcca aggaaaaatt atctcaaact atgtttctga aaaattaaat
    10021 gctaagaaag ttgttcttta cactgacaat gccagtgact atgctaaagg gattgccaaa
    10081 tctttccgcg agtcatacaa gggtgaaatc gttgcagatg aaactttcgt agcaggtgac
    10141 acagacttcc aagcagccct tacaaaaatg aaagggagag actttgatgc tatcgttgtt
    10201 cctggttact ataatgaggc tggtaagatt gtaaaccaag cgcgtggcat gggaattgac
    10261 aaaccaatcg ttggtggtga tggattcaac ggtgaggagt ttgtacaaca agcaactgct
    10321 gaaaaagcat caaacatcta ctttatctca ggcttctcaa ctactgtaga agtttcagct
    10381 aaagctaaag ccttccttga cgcttaccgt gctaagtaca atgaagagcc ttcaacattt
    10441 gcagccttgg cttatgattc agttcacctt gtagcaaacg cagcaaaagg tgctaaaaat
    10501 tcaggtgaaa tcaagaataa ccttgctaaa acaaaagatt ttgaaggtgt aactggtcaa
    10561 acaagcttcg atgcagacca caacacagtc aaaactgctt acatgatgac catgaacaat
    10621 ggtaaagttg aagcagcaga agttgtaaaa ccataataga aaaatgttga aatagggaat
    10681 gagcctttga ctcactccct gtttcgatat ttaatactct tcgaaaatct cttcaaactg
    10741 cgtcaacgtc gccttggatt atatatgtga ctgacttcgt cagtcttatc tacaacctca
    10801 aagcagtgct ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
    10861 tataagaacc tatcaaaaag tgagggaaaa ccctcggaat tataaataga aagagtgaat
    10921 cttatgctcc aacaactcgt aaatggtttg attctaggta gtgtttacgc gctgttagcc
    10981 ctaggatata ccatggttta cggaattatc aagctcatca acttcgccca tggtgatatt
    11041 tatatgatgg gagcctttat cggttatttc ttgatcaatt ctttccaaat gaatttcttt
    11101 gtagcgctta ttgtagctat gctagcgaca gctattcttg gtgtcgtgat tgagtttctt
    11161 gcttaccgac ctttgcgcca ctctactcgt attgctgttt tgattacggc tattggggtt
    11221 tctttcctat tggagtatgg aatggtctat ctggttggtg ccaatacccg tgccttccct
    11281 caagcgattc aaacagttcg atatgatttg ggagcaatta gcttaacaaa tgtgcagtta
    11341 atgattttgg ccatttcctt gattttgatg attttgttac aagtcattgt ccaaaagact
    11401 aagatgggga aagccatgcg tgcagtatca gtagatagcg acgcggcaca attgatgggg
    11461 atcaatgtaa accgtacgat tagctttacc ttcgctttgg gttctgctct tgcgggtgcg
    11521 gctggtgttc tgattgctct ttattataac tctcttgagc ctttgatggg ggttactcca
    11581 ggtcttaaat ctttcgttgc cgcagtactt ggtggtatcg gaattattcc cggtgcggct
    11641 cttggtggct ttgtgattgg tctattggaa acctttgcga cagcctttga gatgtcagat
    11701 ttccgtgatg ccattgttta tggaatcttg ttgttgatct tgattgtccg cccagctggt
    11761 atccttggta agaatgtgaa agagaaggtg taaacgatga aggaaaattt aaaagttaat
    11821 attctatggt tactcctttt gttagctggc tatagcttga ttagtgtact ggtttcagtc
    11881 ggagtactta atctattcta tgtacagatt ttacaacaaa ttggaattaa tattattttg
    11941 gctgttggtc tcaacttaat cgttggtttt tcaggacaat tttcacttgg tcatgctggt
    12001 ttcatggcga ttggtgccta tgcagcagct attattggtt ctaaatcacc aacctacggt
    12061 gccttctttg gagctatgct tgtaggggct ttgctttcag gagcagttgc cttacttgtc
    12121 ggcattccaa ccttgcgctt gaagggggac tatcttgcgg tagcaactct gggtgtttct
    12181 gaaattatcc gtatctttat catcaatggt ggaagtctta caaatggtgc ggcaggtatc
    12241 ttagggattc ctaactttac aacttggcaa atggtttact tctttgtcgt gattacaacc
    12301 attgcaacct tgaacttctt gcgtagccca attggccgtt caaccctctc tgttcgtgaa
    12361 gatgaaatcg ctgctgagtc agttggggtt aatacgacta aaattaaaat catcgctttt
    12421 gtctttggtg ccattactgc aagtattgct gggtcacttc aggcaggatt tatcgggtct
    12481 gttgtaccga aagattacac cttcatcaac tcaatcaacg ttttgattat tgttgtattt
    12541 ggtggactcg gttccattac aggtgcgatt gtttcggcta ttgttctggg aattttgaat
    12601 atgcttctcc aagatgttgc tagtgtgcgt atgattattt acgctttggc cttggtattg
    12661 gtaatgattt tcagaccagg tggactcctt ggaacgtggg aactgagcct atcacgtttc
    12721 tttaaaaaat ctaagaagga ggaacaaaac taatggcatt acttgaagta aaacagttaa
    12781 ccaaacattt tggtggtcta acagctgttg gagatgtgac tcttgaattg aacgaagggg
    12841 aactggttgg attaatcggt ccaaacggag ctgggaaaac cacccttttc aaccttttga
    12901 ccggtgttta tgaaccaagc gagggaacag taaccctaga tggtcacctt ttgaatggga
    12961 aatcacctta taagattgcc tctttgggac ttggacgtac tttccaaaat atccgtctct
    13021 ttaaagattt aacagtttta gataatgttt tgattgcttt tggaaaccat cacaaacagc
    13081 atgtttttac tagtttctta cgcttaccag ctttttacaa gagtgaaaaa gaattaaagg
    13141 ataaagcttt ggaattgttg aaaatctttg atttagatgg tgatgcagag actcttgcta
    13201 aaaatctttc ctacggacaa caacgtcgtt tggaaattgt tcgtgccctt gctacggaac
    13261 ctaaaattct cttcttagat gaaccagcag caggtatgaa cccacaggaa acagccgaat
    13321 tgactgagtt aattcgtcgt atcaaagatg aatttaagat tacaatcatg ttgattgaac
    13381 acgatatgaa tctggtcatg gaagtaacag aacgtatcta cgtacttgaa tatggccgtt
    13441 taattgctca aggaactcca gacgaaatta agaccaataa acgcgttatc gaagcttatc
    13501 taggaggtga agcctaatgt ctatgttaaa agttgaaaat ctttctgtgc attacggtat
    13561 gatccaagca gtccgtgatg taagctttga agttaatgaa ggagaagttg tttcccttat
    13621 cggtgccaac ggtgcaggta agacaactat tcttcgcacc ttgtcaggtt tggttcgacc
    13681 aagttcagga aagattgaat ttttaggtca agaaatccaa aaaatgccag ctcagaaaat
    13741 cgtggcaggt ggtctttcac aagttccaga aggacgccac gtctttcctg gcttgactgt
    13801 tatggaaaat cttgaaatgg gagctttctt aaagaaaaat cgtgaagaaa atcaagctaa
    13861 cttgaagaag gttttctcac gctttcctcg tcttgaagaa cggaagaacc aagatgcagc
    13921 tactctttca ggaggggaac aacaaatgct tgccatggga cgcgctctta tgtcaacacc
    13981 aaaacttctt cttttagatg aaccatcaat gggacttgcc ccaatcttca tccaagagat
    14041 ttttgatatc attcaagata ttcagaagca aggaacaacc gtcctcttga ttgaacaaaa
    14101 tgccaataaa gcacttgcaa tctctgaccg aggatatgta ttggaaacag gaaaaatcgt
    14161 cctatcagga acaggaaaag aactcgcttc atcagaagaa gtcagaaaag catatctagg
    14221 tggctaaaac aatccagtgg attgttttag tcggcagatg aagattacga agtaatcatc
    14281 aatatagtcc gggggccctt tttagtcggt ggatgaagat tgcgaagcaa tcatcaaatc
    14341 cagaggattg ttttagccgg tagattgaga ttgcaaacaa atctgcatct acattgaaag
    14401 ctgaatttct aataattgaa aaaatcgaat gaaacgtatc ttaccttcat tcagggagct
    14461 cgattttaga gctctttttg ctagcttatt catacttttt tgaatttcga aaaagaaatg
    14521 taagcgtttg atagatttac aaaaagattg tataatgggg ataagaatag aaaaggagaa
    14581 gtctcatggc agttaaagat tttatgaccc gcaaggtagt ttatattagt ccagatacaa
    14641 cagtatctca tgcagcagat ttgatgagag agcaagaatt gcaccgcttg cctgttatcg
    14701 aaaatgatca attggttggt ttggtgactg agggaaccat tgcacaagca agtccatcta
    14761 aagcaacaag tctttctatc tatgagatga attatcttct gaataagaca aaagtaaaag
    14821 atgtcatgat tcgcgatgtt gtcactgtct caggctatgc tagtctagaa gatgcaactt
    14881 atctgatgtt gaaaaataag attggtattc tccctgtcgt agataaccat caagtatacg
    14941 gagttattac tgaccgtgac gttttccaag cctttcttga aattgcaggt tatggcgaag
    15001 aagggattcg tgtacgcttt gttacagaag atgaagttgg tgttcttgga aaaattgttt
    15061 ctttgattgt agaagaaaat ttgaatatct cccatacagt caatattccg cgtaaggatg
    15121 gtaaggtcat tatcgaagtg caaatcgatg gatcaattga tttaccagcc ttgaaagaaa
    15181 aatttgaagc aaatggtatt caagtggaag aaatcgctcg cacttcagca aaagtcttgt
    15241 aagaagggaa gcccaaaggc ttcttttttc atgaaaaggg gattagagaa aaagatggaa
    15301 agaaatgata aaatatgcta taatgaaata atgtaaaaaa ggagtattta tggacatttc
    15361 agtaattcgt cagaaaattg acgcaaatcg tgaaaaatta gcttctttca gggggtctct
    15421 ttgacctcga agggctagag gaagagattg ccatcttgga aaacaagatg acagaacctg
    15481 atttttggaa cgataatatt gcggcccaaa aaacgtcgca agaattaaat gaattaaaaa
    15541 acacttacaa taccttccat aagatggaag agttgcagga tgaagtcgaa attttattgg
    15601 attttttggc tgaagacgag tcagtgcatg atgaactggt agcgcagtta gccgaacttg
    15661 ataagataat gaccagctac gagatgactc tactcttgtc agaaccttat gaccacaaca
    15721 atgccatctt ggaaatccat ccaggttctg gtggtactga ggcgcaggac tggggtgata
    15781 tgttgcttcg tatgtatact cgttatggta atgctaaagg ctttaaagtg gaagtgttgg
    15841 attaccaagc aggtgatgag gctggtatta agtcggtaac tttatcattt gaagggccta
    15901 atgcctatgg tctcctcaag tcagaaatgg gtgttcaccg cttagtgcga atctcaccat
    15961 ttgactctgc caaacgtcgc catacctctt tcacatctgt agaagtgatg ccagaattgg
    16021 atgatactat tgaagtggaa atccgtgaag atgatatcaa gatggatacc ttccgttcag
    16081 gtggtgccgg tggacaaaac gtcaataagg tttcaacagg tgtacgttta acccacattc
    16141 caactggaat tgttgtccaa tcaacagtgg atcgtaccca gtatggaaat agagatcgtg
    16201 ccatgaagat gttgcaggct aagctctatc aaatggagca agataagaag gctgcggagg
    16261 tagattctct caaaggtgag aaaaaggaga tcacttgggg aagccaaatc cgttcttatg
    16321 tcttcacgcc ttatactatg gtaaaagatc accgaactag ctttgaggtt gctcaggtag
    16381 ataaggttat ggatggggac ctagatggtt ttatcgatgc ttatctcaag tggcgaatta
    16441 gctaagatag aaaggaactc acatgtcaat tattgaaatg agagatgtcg ttaaaaaata
    16501 cgacaacgga acaactgctc tacgcggtgt ttcggttagc gttcaaccgg gggaatttgc
    16561 ttacatcgta ggaccttcag gagcagggaa gtcaactttt attcgttctc tgtatcgtga
    16621 agtaaaaatc gataaaggaa gcctatcagt tgctggtttt aatctggtta agatcaaaaa
    16681 gaaagatgtc ccgcttctac gtcgtagtgt tggggttgtc ttccaggatt ataaactgtt
    16741 accaaagaaa actgtctatg aaaatattgc ttacgctatg gaagtaatcg gggaaaatcg
    16801 ccgtaatatc aaaagacgag tgatggaagt tttggacttg gttggattga agcataaggt
    16861 tcgttctttt ccaaatgaac tctcaggtgg ggagcaacag cgaattgcga ttgcgcgtgc
    16921 aattgtaaat aatcccaaag tattgatagc tgatgagcca acaggaaatc tggatccgga
    16981 taattcatgg gaaattatga atctcttgga acggattaac ctacaaggaa caactatttt
    17041 gatggcgact cataatagcc agattgtaaa taccttgcgc caccgtgtca ttgccattga
    17101 aaatggccgt gtcgttcgtg acgaatcaaa aggagagtat ggatacgatg attagtagat
    17161 tttttcgcca tttatttgaa gccttaaaaa gtttgaaacg aaatggttgg atgacagtag
    17221 ctgctgtcag ttcagtcatg attactttga ccttggtggc aatatttgca tctgttattt
    17281 tcaatacagc gaaactagct acagatattg aaaataatgt ccgtgtagta gtttatatcc
    17341 gaaaggatgt ggaagataat agtcagacaa ttgaaaaaga aggtcaaact gttacaaata
    17401 atgactacca caaggtatat gattctttga agaacatgtc tacggttaaa agtgttacct
    17461 tttcaagtaa agaagaacaa tatgaaaaat taaccgagat aatgggagat aactggaaaa
    17521 tctttgaagg agatgccaat cctctctatg atgcctatat tgtagaggca aacactccaa
    17581 atgatgtaaa aactatagcc gaagatgcta aaaaaattga aggtgtctct gaggttcaag
    17641 atggcggtgc caatacagaa agactcttca agttagcttc atttatccgt gtttggggac
    17701 tagggattgc tgctttgtta atttttatcg cagttttttt gatttcaaat accattcgta
    17761 ttaccattat ttcccgcagt cgcgaaattc aaatcatgcg cttggtcgga gctaaaaaca
    17821 gttatatccg tggaccgttc ttgttagaag gagcctttat cggtttattg ggagctatcg
    17881 caccatctgt tttggtcttt attgtttatc aaattgttta tcaatctgtc aacaaatcgt
    17941 tggtagggca aaatctatcc atgattagtc cagatttatt tagtccgttg atgattgccc
    18001 tactatttgt gattggggtt ttcattggtt cattgggatc aggaatatcc atgcgccgat
    18061 tcttgaagat ttaggtaaaa tagctgcttt tatgaggaga ttgtaaaatc tccttttttg
    18121 ctacaagagt ttttgaaaag agatgcgcag aagaaaagag cttccaaaga agtcccccag
    18181 agaagacttc ttgatggcta atttgaactt gaaatttgac tcactagaaa ttttttagaa
    18241 aaaagt
//
LOCUS       DAVMEF010000026        18106 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMEF010000026 DAVMEF010000000
VERSION     DAVMEF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.026"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            329..1375
                     /gene="pheS"
                     /locus_tag="VYQ30_000637"
     CDS             329..1375
                     /gene="pheS"
                     /locus_tag="VYQ30_000637"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1965797.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1375..1884
                     /locus_tag="VYQ30_000638"
     CDS             1375..1884
                     /locus_tag="VYQ30_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965798.1"
                     /translation="MIIKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1961..4366
                     /locus_tag="VYQ30_000639"
     CDS             1961..4366
                     /locus_tag="VYQ30_000639"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1965799.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKTIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4434..5435)
                     /locus_tag="VYQ30_000640"
     CDS             complement(4434..5435)
                     /locus_tag="VYQ30_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1965800.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMASILSFVFFSLSFYNKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATKIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFKRGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5591..6036
                     /locus_tag="VYQ30_000641"
                     /pseudo
     CDS             5591..6036
                     /locus_tag="VYQ30_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6162..6428
                     /locus_tag="VYQ30_000642"
     CDS             6162..6428
                     /locus_tag="VYQ30_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1965801.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEL
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKILAVVRLEQGKS"
     gene            6688..8937
                     /gene="metE"
                     /locus_tag="VYQ30_000643"
     CDS             6688..8937
                     /gene="metE"
                     /locus_tag="VYQ30_000643"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1965802.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEVAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9001..9867
                     /gene="metF"
                     /locus_tag="VYQ30_000644"
     CDS             9001..9867
                     /gene="metF"
                     /locus_tag="VYQ30_000644"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1965803.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10552..12765
                     /gene="pnp"
                     /locus_tag="VYQ30_000645"
     CDS             10552..12765
                     /gene="pnp"
                     /locus_tag="VYQ30_000645"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1965804.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12781..13398
                     /gene="cysE"
                     /locus_tag="VYQ30_000646"
     CDS             12781..13398
                     /gene="cysE"
                     /locus_tag="VYQ30_000646"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1965805.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13410..14294
                     /locus_tag="VYQ30_000647"
     CDS             13410..14294
                     /locus_tag="VYQ30_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965806.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14381..15724
                     /locus_tag="VYQ30_000648"
     CDS             14381..15724
                     /locus_tag="VYQ30_000648"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1965807.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15717..16103
                     /locus_tag="VYQ30_000649"
     CDS             15717..16103
                     /locus_tag="VYQ30_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1965808.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16107..16991
                     /locus_tag="VYQ30_000650"
     CDS             16107..16991
                     /locus_tag="VYQ30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1965809.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17128..17727
                     /locus_tag="VYQ30_000651"
     CDS             17128..17727
                     /locus_tag="VYQ30_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965810.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            17870..18106
                     /locus_tag="VYQ30_000652"
                     /pseudo
     CDS             17870..18106
                     /locus_tag="VYQ30_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5438 a         3493 c         4115 g         5060 t
ORIGIN      
        1 atcttatttc aatccgctat aaagttagat aagaataagc taacaagcag aagtaagata
       61 ggtcttgcaa gaatggtaaa aatctgataa actaagtagt aattgaatag gaatctgaaa
      121 gactagtagc ccaagggaag tatatcaggg aggagagccg tgactgcaag ctctctatat
      181 gaaagctggg tgaattcact tgcgcatgga tttagaaatg aaggtctgac ttgtcagatg
      241 aaaacggatg gtaccgcgtg tcaacgctcc gagtggagtt tttggcatgt ggttttcttt
      301 ttatctacga gagactgatg gaggaaatat gtcaactatt gaagaacaat taaaagcgct
      361 tcgcgaagaa acgctggcta gcttgaagca gattactgct ggaaatgaaa aagagatgca
      421 agatttgcgt gtctctgtcc ttggtaaaaa gggttcgctc actgaaatcc tcaaagggat
      481 gaaagatgtt tctgctgaga tgcgtccaat catcgggaaa cacgtcaatg aagctcgtga
      541 tgtcttgact gctgcttttg aagaaacagc taagctcttg gaagaaaaga aagtcgcggc
      601 tcaactggct agcgagagta tcgatgtgac gcttccaggt cgtccagttg cgactggtca
      661 ccgtcacgtt ttgacacaaa ccagtgaaga aatcgaagat atcttcatcg gtatgggtta
      721 tcaagtcgtg gatggttttg aagtggagca agactactat aactttgaac gtatgaacct
      781 tccaaaagac cacccagctc gtgatatgca ggatactttc tatatcactg aagaaatctt
      841 gctccgtacc cacacgtctc cagttcaggc gcgtgctatg gatgcccatg atttttctaa
      901 aggtcctttg aagatgatct cgccagggcg tgtcttccgt cgcgatacgg acgatgcgac
      961 ccacagtcac caattccacc aaatcgaagg cttggtagtt gggaaaaata tctctatggc
     1021 tgatcttcaa ggaacgcttc agttgattgt ccaaaaaatg tttggtgaag agcgtcaaat
     1081 tcgtttgcgt ccatcttact tcccattcac agagccatct gttgaggtgg atgtttcttg
     1141 cttcaagtgt ggtggagaag gctgtaatgt atgtaagaaa acaggttgga tcgaaattat
     1201 gggggccggt atggttcacc cacgtgtcct tgaaatgagt ggtatcgatg cgactgtata
     1261 ctctggcttt gcctttggtc ttggacaaga gcgtgtagct atgctccgtt atggaatcaa
     1321 cgatatccgt ggattctacc aaggagatgt ccgcttctca gaacagttta aataatgatt
     1381 ataaaagtag aaatggcaga tgttgaggtg ttggctaaaa ttgccaaaca aacctttcgt
     1441 gaaacctttg cgtatgataa tacggaagag cagttacagg aatactttga agaggcttat
     1501 agtctgaaaa ctttgtcaac tgagttggga aatcctgact ctgaaaccta tttcattatg
     1561 catgaggagg agatagctgg ttttctcaaa gtcaactggg gaagtgctca aactgagaga
     1621 gaattagagg acgcttttga aattcaacgc ctctatgtgc tacaaaaatt ccaaggattt
     1681 ggactaggta agcaactgtt tgaattcgca cttgaacttg ctacaaaaaa tagtttttct
     1741 tgggcttggt taggtgtttg ggagcataat acaaaagctc aagcctttta taatcgatat
     1801 ggttttgaaa aatttagcca acatcatttt atggttggtc aaaaagtaga tacggattgg
     1861 ttactgagaa agaaattaag gtaagaagag gattcttcta ttgaaatgat aggaaaggaa
     1921 aagaaccagt gtggcaatgg tcattctgga gaactaaatt atgcttgtat cttataaatg
     1981 gttaaaagaa ttggtggaca ttgatgtgcc atcacaggag ttggctgaaa aaatgtcaac
     2041 cacagggatc gaggtcgaag gtgtcgaatc accagctgct ggtctctcaa aaattgtcgt
     2101 cggtgaggtc ttgtcttgcg aagatgtgcc agagactcac ctccatgttt gtcaggttaa
     2161 cgttggcgaa gaagaagccc gtcagatcgt ttgtggtgcc ccaaatgtgc gtgctgggat
     2221 caaggtcatg gtggctcttc caggagctcg tatcgctgat aactacaaaa tcaaaaaagg
     2281 aaaaatccgt ggtttggagt cacttggaat gatctgttca cttggtgaat tgggaatttc
     2341 tgactcagtt gtgcctaagg aattcgcaga tggcatccaa atcttgccag aaaatgccgt
     2401 gccaggtgag gaagtctttt cttacctaga cttggatgat gaaatcatcg aactttccat
     2461 cacaccaaac cgtgcagatg ccctttctat gtgtggagtg gctcacgaag tggcagccat
     2521 ctatgacaag gcagtcaact ttaaagaatt tactctaaca gaaactaatg aagctgcggc
     2581 agatgccctt tctgtcagca ttgggacaga caaggcgcct tactatgcag ctcgtatctt
     2641 ggacaatgtg actatcgcac caagtccaca atggttgcaa aaccttctca tgaacgaagg
     2701 catccgtccc atcaataacg ttgtagacgt gacaaactac atcctgctct actttggtca
     2761 acctatgcat gcttttgact tggacacatt tgaagggact gacatccgtg tgcgtgaagc
     2821 gcgtgctggt gaaaaattgg tgaccttgga cggtgaagaa cgtgacttgg acgtgaatga
     2881 cctagtcatc actgttgcag acaagccagt agcccttgca ggtgtcatgg gtggtcaagc
     2941 aacagaaatc tctgaaaaat ctagtcgtgt tgtccttgaa gctgctgttt tcaatggcaa
     3001 aactatccgt aagacaagtg gtcgcctgaa ccttcgttct gagtcatctt ctcgctttga
     3061 aaaaggaatt aatgtggcaa cagttaatga agcccttgat gcggcagcta gcctgattgc
     3121 ggaacttgca ggtgcgacgg tgcgtaaggg tattgtttca gcgggtgagc ttgatacttc
     3181 agatgtagaa gtttcttcaa cccttgctga tgttaaccgt gtcctcggaa ctgagctgtc
     3241 ttatgctgat gtcgaagacg tcttccgtcg tcttggcttt ggtctttctg gaaatgcaga
     3301 cagctttaca gtcagcgtac cacgtcgtcg ttgggatatc acaatcgaag ctgacctctt
     3361 tgaagaaatt gctcgtatct atggttatga ccgcttgcca actagtctac caaaagacga
     3421 tggtacagca ggtgaattga cagccacaca aaaactccgc cgtcaagttc gtactattgc
     3481 tgaaggagca ggtttgacag aaatcatcac ctatactcta acaactcctg aaaaagcagt
     3541 tgagtttacg gctcaaccaa gtaaccttac ggaactcatg tggccaatga ctgtggatcg
     3601 ttcagtcctc cgtcaaaata tgatttcagg tatccttgat accgttgcct acaacgtggc
     3661 tcgtaagaat aaaaacttgg ccctttacga gattggaaaa gtctttgaac aaacaggtaa
     3721 tccaaaagaa gaacttccaa atgagatcaa cagctttgcc tttgccttga caggcttggt
     3781 tgctgaaaaa gatttccaaa cagcagcagt tccagttgat ttcttctatg ctaagggaat
     3841 ccttgaagcc ctatttgctc gtttgggact ccaagtaacc tatacagcaa catctgaaat
     3901 cgctagcctt catccaggtc gtacagccgt gatttcactc ggtgaccaag ttcttggttt
     3961 ccttggccaa gtgcatccag tcactgctaa ggcttatgat attccagaaa cgtatgtagc
     4021 tgagcttaac ctttcagcta tcgaagctgc gcttcagcca gcggctccat ttgtagaaat
     4081 caccaaattc ccggcagtca gccgtgacgt tgcccttctc ctcaaggcag aagtgactca
     4141 tcaagaagtt gtagatgcta tccaagctgc cggcgtgaaa cgtttgacag atatcaaact
     4201 ctttgacgtc ttctcaggtg agaaattggg acttggtatg aagtccatgg cttatagctt
     4261 gaccttccaa aatccagaag atagcttaac ggacgaagaa gtcgcacgct atatggaaaa
     4321 aatccaagct tctctcgaag aaaaagtcaa tgcagaagtg cgttaaacga tagtatgata
     4381 caaatcaccc ctctagttta tgaacactag aggggtgatt ttatataaaa taattaaaat
     4441 gtgatttctg taaaaataca tctatgatca gagttttgaa aacttacaat gtctaaatct
     4501 ttaacatagt agtggttttt aggcaataaa atatgatcta ttgttgcatt aaaaagtttt
     4561 ggactttggc tattccaagt tcctctttta aagggtggaa gtgcattcaa tgcgtcccta
     4621 tgagagctta tttttgcaag tgctccatga cgcatagttg cattaaaatc acctgcaata
     4681 atagcctttg gatattttga agccaattga ttatgaatga tgtttaagtc ttgcttccag
     4741 atttccatta aacctggcag aggaggagca gtatgcaagg caatgatatc tggtatattt
     4801 tgttttctcg aatgtaatac aattgtcccg aaccgtgttg tatgaaaagt tttagcttct
     4861 gtatagaaac cataactttt cttgacaatc acagtcacag gagctattcc actattggta
     4921 ggtggagaag tgaaaatatc atagttggcc atagaaagtc caacttgatg aaacaatagt
     4981 ttgattctct ggttttcttg ctcacctctg atcttggtag ctagttcagg gaatatagcc
     5041 atatcggcat caaaatggct aaaaattcgc tcaatatgtt gtgcttctat ttgattagcg
     5101 acgttccaag ttactaattt taaggtttta gtcttatttg atgcagaaga aagggtttta
     5161 tctgttccga ataatagtaa tatgagcgat agtaagagca aaatgccaga gtaaaaccgt
     5221 atttgtttat ttttattgta aaaagatagg gaaaaaaata cgaaacttaa aatagaagcc
     5281 atagttgcag tagctaaggg gaaggcataa atctgtgcca agataggtct ggtagatagc
     5341 caaatattaa gattagaaga tggaaaaaaa gaataagtta tgatagggat aacaaagaga
     5401 atgaccgaaa gggcagagag aaatgtttta atcattgtgt gagtacctca atttgcaagg
     5461 gttgctcgga gctaatgtga ctttgtgact ttacttgatt gatattcttt ttcttgtctt
     5521 cctcatagat taagtacaac ccaagtaaaa aaagtccaat ttaagattga aatgattaaa
     5581 aggcacagag atgcaagaca attatactac aaaaggtaaa catttgacaa tcgatagccg
     5641 tcgcttaatc gaaagatgga aaaaagaagg aaaatcaaat agagaagttg cctctctact
     5701 tggaaaagct cctcaaacta tccacactga aatcaagcgt gggacagtcc gaaaatgtct
     5761 tggaaaaggg cgcttcaaag aggtttattc tgccgactac gctcaacagt gttatgaaaa
     5821 caatcgcaag cgctcggtca agaaatcaag cttgaccaag gaactaaagg aaaagattcc
     5881 actatacata accaaaaatt ttcgcctgaa atgatggtta tggctaaagg ggttaacgtg
     5941 ggaatttcaa ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat
     6001 ctgctttatc ctagaaaagg aaaatcgctt aagaaatagg ctagcaccaa ctttaaaccc
     6061 gctggtcaat ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaag
     6121 aaagagggtt aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg
     6181 agctctttag caaggaggaa atccttgaaa gtgatatgcg agtagctatc atgagcgagt
     6241 tgattgaagc cagaaataag caaggaatca gtcagaaaaa gctagaggaa ctcagtggag
     6301 tgagtcagcc tgttatagct aggatggaga caggtaagac tagtccacag ttggacacag
     6361 tcttaaaagt cctagctagt ctaggaaaga tactagcagt cgtccgactt gaacagggga
     6421 aaagttgact agaatcagtt atagtctttt ctaataacaa gccatagtca cttataagaa
     6481 ttactaataa cgcttttgag cattctaact aaaatacagc taaaaacaag ttataaaagg
     6541 gatttgaggc atgtgtctca agtctttttc ttttgtctaa aatagaggta tagtttcaaa
     6601 ctatatcaaa gcctaatttt ttacaattat acagatgatt tcttttctgt taagatagtt
     6661 tcaacaacaa attttggagg acacatcatg tcaactacaa tcatcggttt ccctcgtttg
     6721 ggcgaattcc gtgaattaaa atttacaact gaaaaatact ttagaaaaga aatctcagaa
     6781 gaagaacttt tagcagcagc aaaagacttg cgtgctaaac actggaacat tgtcaaagaa
     6841 aaaggcatca ctgaaattcc atcaaatgac ttttctcact atgataactt cctagatgcg
     6901 gctttccttt tcaacgtggt gcctgcatcc gttcaaaact tggacttgtc tgaccttgag
     6961 cgctacttcg ctttgggacg tggttaccag ggagaaaaag gggacgttcg cgcccttcca
     7021 atgaagaaat ggttcaacac taactaccac tacatcgttc ctaaatttga aaaagacact
     7081 caagtaaaat tggctggtca caagattttc gatgagttcc aagaagcaaa agaacttgga
     7141 ttgaacactc gtcctgttct tgtaggtcca ttcactttcc ttcaattgtc agactttgaa
     7201 gaaggcgtga aagcagacga cttcgtagat agcttagtgg ctgcttacca agaagttttt
     7261 gctaaattgg ctgaacttgg tgcgactcgc atccaattgg atgaagcggc tcttgtaaaa
     7321 gacttgacag ctgaagaaaa agctctcttc ttgaaccttt acaacaaact cttggctgac
     7381 aaaaaaggtc ttgaagtctt gcttcaaact tatttcggtg atgttcgtga cgtatacgct
     7441 gaccttgtaa acttgccagt agatgctatc ggtcttgact ttgttgaagg taagaaaact
     7501 cttgaactcg ttaaaggtgg cttcccagct gacaagactc tctatgtagg tattgtcaat
     7561 ggtaaaaaca tctggcgcaa caactacgaa aagagcttgg ctgttcttga gcaaatccca
     7621 gctgaaaaca tcgttttgac aagctcttgc tcacttcttc atgtgccatt tacaactgct
     7681 aatgaagaat ttgaaccagc actcttgaac cactttgcct ttgcagttga aaaattggat
     7741 gaaatccgtg acttggatgc tatccgcaat ggtcaaggtt cagaagctct tgcagccaac
     7801 aaagaactct ttgcgactga gcgtgttggt gaaaatgctg aacttcgtgc gcgtatcgct
     7861 ggcttgacag acgcagacta cactcgtttg ccagcctttg cagaacgtga agctatccaa
     7921 gaagaagctt tcaaacttcc agctcttcca acaacaacga ttggttcatt ccctcaaaca
     7981 aaagaagttc gtgccaaacg tttggcttac cgtaaaggtg aattgtctca aaaagagtac
     8041 gacgctttcc ttgctgaaac gattgacgaa tggatcaagt ggcaagaaga tattgacttt
     8101 gatgtccttg ttcacggtga atttgagcgt aatgacatgg ttgagtactt cggtcaaaac
     8161 ttgtcaggtt acctcttctc taaaaatggt tgggtacagt catacggtat gcgtggggtg
     8221 aaaccaccaa tcatctgggg tgatgtcact cgtcttaacc ctatcactgt taaatggtct
     8281 agctatgcac aaagtcgtac aaacaaacct gttaaaggta tgttgactgg acctgttacc
     8341 attcttaact ggtcattccc acgtgaagac atctctatca aggattcaac tcttcaaatc
     8401 gcccttgcta tcaaggatga agtacttgac cttgaagttg ctggtgtgaa gatcatccaa
     8461 atcgatgagg ctgctcttcg tgaaaaattg ccactccgtc gtagcgactg gtacgaagac
     8521 taccttgact gggcaattcc tgccttccgc ttggtacact caacagtagc gccagacaca
     8581 caaatccaca ctcacatgtg ttactcagaa tttacagata tcatcccagc tatcgataac
     8641 atggatgcag acgttatttc ctttgaagct agccgttcaa accttgaaat cttggacgaa
     8701 ctcaaagcga aaaacttcca aacagaagtg ggacctgggg tttacgatat ccactcacct
     8761 cgtgtaccaa atgaaggcga aatcgacaac acaatcgaag ccatccttgc taaagtgcca
     8821 agcaagaaag tttggatcaa ccctgactgt ggtttgaaaa cacgtggtat cccagaaaca
     8881 aaagaaagct tgatccgcct tgttgaagca gctaaagctg cgcgtgagaa attgtaagat
     8941 tggatttctc tccatacagt ggttgttcaa aaagaaatca actagaaaag gttattacat
     9001 atgtcacgcc aaacaccgtc actctcattt gaagtgttcc ctccaaaccc agccgtgggt
     9061 aatggtaaca ttatttcagc cttgcaggat atgcaggagt tggctcccca ttttatcagt
     9121 gtaactgcca gcaataataa atttaatatc aaggaaacga cggttcgttt ggctgacttt
     9181 atccaaaatg acttggcgat tccgaccatt gcccacttgc cagctattta tctaactaag
     9241 gacaaggttg ctgaaactat tgctgacttg gacaaagttg gggtgcagaa aatcttggct
     9301 cttcgtgggg atattattcc agacgtagaa ccacaaaagg atttccgcta tgcaaccgac
     9361 ttgattgagt ttatcaagga acaagcccct cactttgata ttattggagc ttgctatcca
     9421 gaagggcatc cagattcgcc aaatcagatt tcagatattc aaaatcttaa gaagaaagtg
     9481 gatgcaggct gttcgagcct cgtaactcag ctcttctttg acaatgagcg cttctatgat
     9541 tttcaggaca agtgtatctt ggctgggatt gatgttccca ttcatgcagg aattatgcca
     9601 attctgaatc gaaatcaggc tctccgactc ttgaagactt gtgagaatat ccatcttcca
     9661 cgcaaattta aagccatctt agacaagtat gagcatgacc ctgagtcgct cagagcagca
     9721 ggacttgcct atgcagtgga ccaaatcgtg gacttggtaa ctcaggatgt tgccggtgtg
     9781 catctctata cgatgaataa tgctgataca gcaaaataca tccatcaagc aacccatgcc
     9841 ttgtttaatc accagtctct aggataataa aagcaaacca ttcttctcag gtgaggggaa
     9901 tggttccttt ttaatggtaa agacctcact ttttaagaaa aatatgataa aataaattct
     9961 gtacgtactt gatacaaaga tgaaggtatt aaataataca atgcatttaa actgtaagat
    10021 ttaacgacac gagtgagtca aatcgatgtt atttgacgag agagttagta aagcatttag
    10081 ctaatcgccc gatagcgatt agcgtgaaag ctttagatac tttagtatta aacctttcta
    10141 atgatgtatc ttgaaaactg aacttgccta caactctgtg gaaaaagata aatctcccta
    10201 ggagtagtcg ctccgtcgtt cgatttccta tcttcctttg agttgcttga cggcttggta
    10261 tcttgatgac ttgaattttg gtctcatttc ttaggaaaaa agataagctt cctagcactc
    10321 gtcgagtgtg acgtcacctt cctatttttc tacagaaatg ttcggcaagc cgaaccgtcc
    10381 aaaatatctt gtgtaattga acacggccga aaagctgtgt aaaaaagata aactgtcttg
    10441 tcttcatcga agacttcgtc agtttcctat ttttactttg cttttgacgt ccttggtatc
    10501 ttgatctttg taggcaaggc gtataatttc atcaatccaa aggggattaa aatggcaaaa
    10561 caagtgtttc aaacgacttt tgcgggtcgt gagttaattg tagaaactgg tcaggttgct
    10621 aagcaagcaa atggctctgt tgtcgtacgt tacggtgagt caactgtctt gactgctgcc
    10681 gttatgtcta agaaaatggc aactggggat ttcttcccac tccaagtcaa ctacgaagaa
    10741 aaaatgtatg cggctgggaa gtttcctggt ggctttatga aacgtgaagg acgtccttca
    10801 acagatgcga ccttgacagc gcgtttgatt gaccgtccga ttcgtcctat gtttgcggaa
    10861 ggtttccgta atgaagtcca agtcatcaat acagtgcttt cttatgatga aaatgcatct
    10921 gcaccaatgg ctgctatgtt tggttcatct ttggcactgt ctatttcaga tattccattt
    10981 gacggaccaa ttgctggggt acaagtggga tatgtagatg gccaaatcat catcaaccca
    11041 agtcaagaac aagcagagca atctcttctt gaattgacag tagctggaac caagcacgct
    11101 atcaacatgg tagagtctgg tgccaaagaa ttgtcagaag aaatcatgtt ggaagcgctt
    11161 cttaaagggc acgaagctgt caaagaattg attgccttcc aagaagaaat cgttgctgct
    11221 gtcggtaaag aaaaagcaga agtggaattg cttcacgtgg atgctgaatt gcaagctgaa
    11281 atcattgcag cctacaacag tgacctccaa aaggcagttc aagtagaaga gaaattggcc
    11341 cgtgaagctg caactcaagc agtgaaagac caagtgactg ccgtttacga agaaaaatat
    11401 gcgaaccacg aagaatttga ccgtattatg cgtgatgtgg ctgaaatctt ggaacaaatg
    11461 gaacacgcag aagtgcgacg tttaattaca gaagacaagg tgcgtcctga tggtcgtaag
    11521 gtcgatgaaa tccgtccttt ggatgcggtt gttgacttcc ttcctcgtgt acatggttca
    11581 ggtctcttta ctcgtgggca aactcaagct ctttcagtct tgaccttggc tccgatggga
    11641 gaaactcaaa tcattgatgg tttggatcca gagtacaaga aacgctttat gcaccactat
    11701 aacttccctc aatattctgt aggggaaaca ggtcgttacg gtgcgccagg tcgtcgtgaa
    11761 atcggtcacg gtgcccttgg tgagcgtgct cttgctcaag tcttgccaag cttggaagaa
    11821 ttcccatacg ctatccgtct agtagcagaa gttttggaat caaacggttc ttcatctcaa
    11881 gcttctatct gtgcgggaac tcttgccctt atgactggtg gtgtgccaat caaggcgcca
    11941 gtagctggta ttgctatggg acttatctca gatggaaata actacacagt attgacagat
    12001 atccaaggtt tggaagatca ctttggagat atggacttca aggttgcagg tactcgtgat
    12061 gggattacag cccttcaaat ggatatcaag attcaaggga ttactgcaga aatcttgacg
    12121 gaggctcttg ctcaagccaa gaaagcgcgt tttgaaatcc ttgatgtcat tgaagcaacc
    12181 attccagaag ttcgtccaga attggctcca actgctccga aaattgatac gatcaagatt
    12241 gatgtggaca agattaagat tgtcatcggt aagggtggag aaaccatcga caagattatc
    12301 gctgaaacag gtgttaagat tgatatagac gaagaaggaa atgtgtctat ctactctagt
    12361 gaccaagatg ctattaaccg tgccaaagaa attattgctg gtttggttcg tgaagccaaa
    12421 gtggatgaag tttaccgtgc taaagtcgtt cgtatcgaga aatttggtgc ctttgttaac
    12481 ctctttgata agacagatgc ccttgttcat atctctgaga tggcttggac tcgtaccaat
    12541 cgtgtagagg atttggtaga aatcggggat gaagttgatg ttaaggttat caagattgat
    12601 gaaaaaggcc gtatcgatgc ctctatgaag gctcttctac ctcgtccgcc aaaacctgag
    12661 catgatgaaa aaggtgaaaa gtctgagcgc cctcaccgcc cacgtcatca caaggatcac
    12721 aaacctaaga aagaatttac agaaacacca aaagattcag agtaagaaaa ggagaaatgt
    12781 atggggtggt ggcacgaaac cattgatatc gtaaaagaaa atgatccagc ggcccgcacc
    12841 actttggagg ttttgctgac ttatccaggt gtcaaggcct tggcggccca ccgtctctcg
    12901 cattttctct ggaagtacga cttcaaactc ttagctcgta tgcacagtca gttttggcgc
    12961 ttttggactc agattgagat tcatccagga gcccagattg attcaggtgt ctttattgac
    13021 catggttctg gtctggtgat tggagagaca gcgattgttg aaaaaggcgt tcttctctat
    13081 cacggagtga ctctcggggg aacagggaaa gactgtggca aacgccatcc gactgtacga
    13141 aagggagccc tcatatcagc ccatgcccaa gttatcgggc ctgtggaaat tggtgaaaat
    13201 gccaaggtcg gtgctgcagc agttgtcgta gcagatgtac ctagtgatgt gacggttgtc
    13261 ggtattccgg ccaagattgt ccgtcttcat ggtaagaaag atgagcctgt tattcacgaa
    13321 gtcgaagaaa aacgggagta ttatgtcaat aaactcgagc aggctaaaga tgccagtcac
    13381 agatcgtctg gtttgtagag gatacctata tgattcaagc aagaaacaag ttaagccaag
    13441 aggagctatc tgaggcgaaa aaagtaatta actgttgcca aaactatgac ggtacctatc
    13501 gcgatcccta tctctctaac atgcttaatt ttgacccaaa catgcccgcc tttttccttt
    13561 attatgaaaa aggcaaactt gttggtttat taactgtcta tgcagatgac caagatgtag
    13621 aagtgacgat attggttcat ccaggtcatc gccgtcaggg gattgcgcgt gcattgttta
    13681 ctagttttga gagagaaaca gcttctttcc ccattcggtc agtcactttt cagacagaac
    13741 gtattttttt agagaatcat cctgactttg ccagtaactg gggactaatc gaggatgaag
    13801 agacagaaac ttggttaggt aaggatagaa gaccttatcc gttagcaaat gtttcccatc
    13861 ttgaagtttt gttagcagat agttcgtatc aggatcaaat tagtcaatta aaatttcagg
    13921 cattttcaga ggaacatgaa tcgagagaag ttgtggatag atatgtcgct gaagctctga
    13981 aggatccaga aagtcgctta tatattttgt taaaagacgg tcaggttatt gggacttgca
    14041 cggtagattt atcgactaat acgaattact tctacggttt agcaatatcg gaacctgaac
    14101 gtgggaaagg ctatggaagc tacttagcaa aatccctcgt caaccaacta attgagcaaa
    14161 atgacaagga atttcagatt gcagtggaag atagcaatgt aggtgccaaa cgtttgtatg
    14221 aaaaaattgg ctttgtcaaa cagacacagg tggtttatct gaatgagaaa ggagcaaggg
    14281 attccgaagt gtagagacat tcggactgaa atttacttga gctcttagtt tagtgatgaa
    14341 actaacagtt cttggatttt agctttcctg attatgattt atgattaaaa tctatgacac
    14401 catgtctcgt gatttgcgag aatttgtccc gattgaggac ggcaagatca agatgtatgt
    14461 ttgtgggcca accgtgtaca actatattca cgtgggaaac gcccgttcga cggtagcttt
    14521 tgatacgatt cgtcgctatt ttgagtaccg tgggtataag gttgcctata tttccaattt
    14581 tacagatgtg gatgataaga ttatcaaccg tgccagggaa gaaggcatca cacctcagga
    14641 ggttgcggat aagtacatcg ctgcctttcg tgaggatgtg acggccttag gcgtgaaacc
    14701 tgcgactcgc catccgcgtg tagtggagtt tatggcagac atcatccgtt ttgtggaaga
    14761 cttgatcgag aaaggctttg cctatgagag tcaaggggat gtctatttcc gtgtagaaaa
    14821 atcccacaac tatgctaaat tggctaataa aaccttggaa gatttggagc taggtgcttc
    14881 aggtcgtacc gatgaagaaa cggctcgtaa ggaaaatcct gtagattttg ctctatggaa
    14941 atcttccaaa ccaggtgaga tttcttggga tagtccttgg ggacctggac gtccgggttg
    15001 gcatattgag tgttcagtca tgtcgacaga gattttaggt gataccattg atatccacgg
    15061 tggtggagct gacctagagt ttccacacca caccaatgaa attgcccaat ctgaagctaa
    15121 aacaggcaag gcttttgcta actactggat gcacaatggc tttgtcaata tcgacaatgt
    15181 caaaatgtct aagtccttgg gtaacttcat tacggtacac gatgccctta aaactcttga
    15241 tgggcaagta cttcgtttct tctttgcgac ccagcactac cgtaagccta tcaacttcac
    15301 ggaaaaggca gttcgcgatg ccgagaccaa tctcaagtat ctgaagaata cttacgaaca
    15361 accatttaca ggaaacgtgg atgcccaaga gttacaaaac tttaaagata agtttgtagc
    15421 agctatggat gaagatttta atgctgccaa cggtatcaca gttgtctttg aaatggccaa
    15481 atggatcaac tcagggaact atgatgcaag tgtcaagcaa gctcttgcag atatgttaga
    15541 aatttttgga attgtctttg ttgaggaagt tttggatgca gagattgaag acttgattca
    15601 aaaacgccaa gaggcgcgtg ccaatcgaga ctttgagaca gcagaccaaa tccgtgacca
    15661 attggttact caaggaatta agctccttga taccaaggat ggagtgaggt ggacacgtga
    15721 ttgatgtcaa tctcattaac gggattgcgc tagcctttga gggggatgcg gtgtattcta
    15781 tgtatattcg ccgtcacctc atcctcaaag gtatgaccaa acccaataaa ctccatcaag
    15841 aagcaaccaa gtatgtgtca gccaaggctc aggctcgcct gattgctctc atgttggagg
    15901 agcaggtcct aacggaaaaa gaagaagaaa tctacaaacg tggccgcaat accaatagcc
    15961 acacaaaggc taaaaatgca gatgtcgtga cttatcgtat gtccacggga tttgaagcgg
    16021 ttatgggcta tctccatatg actgagaatc tggaacgtct tgagagtttg gtttcatggt
    16081 gcatccaaaa agtggagggc tagaacatga gggaaaaaga actacaagac tggtttcctg
    16141 aggctcggat ttcagaccaa ccagtagaga aagagggcta tctcacgctc cctttagctt
    16201 ctcagcagtg gattttgctg gaggaagctg ggctcagcga gcgtgaaaag cagttggttg
    16261 cccttttgac ccagcaggag caggctcgtt cgctaaaccc ttggtattcc tatctggttg
    16321 aaggcaaggg acaggcaccg caagttttta aaaagattca gttggtttat tgccatcttt
    16381 cttattttca gcaggaaaat ctggcttctt ggctagatat gatgcggact ctttttccga
    16441 attgtcagac agtgctacag gtcggatctc aggattatgt tttcgtgctt caacaagaca
    16501 aatacacttc tgtaagagat attttaagtg atacgattga agcggttgag tatgactttg
    16561 gacttcgtct ttctatcatg ttgggtcagg tttggtctca gacgggacat caagccctat
    16621 cagacttaat caaagctgag cgggatttgt tcaagacatg gtggcgtcag ggtcaccaag
    16681 gtgttcatac tttttctcag ctctatcttt ggagtatggg agaaagactc gtggacttga
    16741 agccaattaa agaatgtcta caccagatga ttttggatca agatcagatt caggaaatca
    16801 ttctctctct ttgggaaaat agtgctgttc tcactaaaac agcccagcaa ctctatctgc
    16861 accgcaattc tctccaatac aagattgata aatgggaaga gttgacaggg cttcagttga
    16921 aagagttgac cgacctgacc ttgtgttatc aattgatttt aggttctttg tcaactatag
    16981 ttggtttgta aagaagttaa tatttggaga agaggattgc catcttctcc atttttatgt
    17041 gcagagttat agtggcttga tgctgggaaa gtacactgtg actgctaaaa cattcctaga
    17101 agctgctttg atttccctaa tctatttatg caaattttat gttattttac tataacagtt
    17161 gttgctaagc caaataatag tggggaagtt catttagacg taagcattga agataatcag
    17221 ggaggtagtg ggtataattt cagttctgtt tcaagtagct cacaaacagc taaatatgaa
    17281 ggaactggtt ataataacaa ttcatcatta tatataacga ttgataaaac gtctgatgca
    17341 acagctcttt tgaaattaaa gttgaataat gttgataatc aacctgctac tgaagttcct
    17401 agttcaggaa ttactgtaaa attaaatgct aaagataatg ctggaaactg gacaagtgct
    17461 tcgaataaaa aagaagtaac agtaaaaatt gtttctgcta aaccgacata tccagacaaa
    17521 atcttagtga aaaatcctga taatataaaa gatacagaaa aaaatgccat tattgaaaaa
    17581 ttgaaagagg caaataaaaa tcatccagca ggagctccaa cctttgctaa aggtgaagga
    17641 gagcatgcaa atgatattgt agcaacttat tcagatggta caacttatta tgtaccgtta
    17701 aatgatgtga caaaatatgc gaggtagtgg ttgtaccact cacttattca cctccccgtg
    17761 atttgtagta gtgataggct ttctaactat tattataaaa caaaataaag agcacaacac
    17821 tttttcattc tgtgttgtgc cttgagtgaa acgaaaggaa tgaattataa atatgaaaag
    17881 tatagtcact agcatagatg agcgcttgcg tactcgccta cgagtgatta tctggaagca
    17941 atggaagaag aaatcgagac gattatgggg attgcttaag ttaggggttc ctaaatggat
    18001 agcagataag gtatctggct ggggcgacca ctatcaatta gtagctcaga agtcggtact
    18061 taaacgtgct atatcaaaac cagcccttga aaaacgtgga ctggtt
//
LOCUS       DAVMEF010000027        17312 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMEF010000027 DAVMEF010000000
VERSION     DAVMEF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.027"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..466)
                     /locus_tag="VYQ30_000653"
     CDS             complement(<1..466)
                     /locus_tag="VYQ30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965811.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEK"
     gene            complement(432..995)
                     /locus_tag="VYQ30_000654"
     CDS             complement(432..995)
                     /locus_tag="VYQ30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965812.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(1005..2993)
                     /gene="uvrB"
                     /locus_tag="VYQ30_000655"
     CDS             complement(1005..2993)
                     /gene="uvrB"
                     /locus_tag="VYQ30_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1965813.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVNNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(3070..4023)
                     /locus_tag="VYQ30_000656"
     CDS             complement(3070..4023)
                     /locus_tag="VYQ30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1965814.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            4193..6358
                     /locus_tag="VYQ30_000657"
     CDS             4193..6358
                     /locus_tag="VYQ30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1965815.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            6358..7098
                     /locus_tag="VYQ30_000658"
     CDS             6358..7098
                     /locus_tag="VYQ30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1965816.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            7247..8734
                     /locus_tag="VYQ30_000659"
     CDS             7247..8734
                     /locus_tag="VYQ30_000659"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1965817.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(8782..10071)
                     /gene="ftsY"
                     /locus_tag="VYQ30_000660"
     CDS             complement(8782..10071)
                     /gene="ftsY"
                     /locus_tag="VYQ30_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1965818.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDIEPAFTEAEEVS
                     QEEAEVESSEESVFQEEDSQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(10075..10893)
                     /locus_tag="VYQ30_000661"
     CDS             complement(10075..10893)
                     /locus_tag="VYQ30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1965819.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(10893..11687)
                     /locus_tag="VYQ30_000662"
     CDS             complement(10893..11687)
                     /locus_tag="VYQ30_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1965820.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIACMEGL"
     gene            complement(11684..15223)
                     /gene="smc"
                     /locus_tag="VYQ30_000663"
     CDS             complement(11684..15223)
                     /gene="smc"
                     /locus_tag="VYQ30_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1965821.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLEIILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHSRLEFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(15214..15912)
                     /locus_tag="VYQ30_000664"
     CDS             complement(15214..15912)
                     /locus_tag="VYQ30_000664"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1965822.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(16095..16166)
                     /locus_tag="VYQ30_000665"
     tRNA            complement(16095..16166)
                     /locus_tag="VYQ30_000665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16131..16133),aa:Arg,seq:ccg)
     gene            complement(16230..17216)
                     /locus_tag="VYQ30_000666"
     CDS             complement(16230..17216)
                     /locus_tag="VYQ30_000666"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1965823.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEVGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKVARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
BASE COUNT         4975 a         3966 c         3158 g         5213 t
ORIGIN      
        1 gtttttcctt tataggcttt ccaagttcaa gtattttttc ttgtccatca aaagtaaaac
       61 ccattttttg atagaaagca atggcgcgct tgttatcttt caatacccat aagaaaattt
      121 cagaaaaatg attaagagca gtcaaagctg ctttcattaa cttttgtgcg ataccttttc
      181 cataatagtc ttttaaaaca tataaagcaa taatttcacc agcttgaata gtctcatcac
      241 gaaaattgcc ataactgata aaaccaacca ccttcacgcc atccatcgca atcaatgtat
      301 tttctggata cttttgacta aagaatcgac atctttctaa tgtcattgta tcctgaaatt
      361 ccgcaggcaa aaggtcatca taagactctc tccacgtttg ccagtgaaca agggatttac
      421 cttctatctc tttaggagtt tccattgatt tgataataac cgtcatttat tttctcccag
      481 tcttctctca aaataccata tttaatacta tcaaaatatt taccttgata ataacgaact
      541 tttggaatat gagcttcttt tttcattctt aatttttcag caagtttcat cataccaata
      601 ttccctgacc aagttgtcaa acccagatgc tccaactcca agtaatcctg aaacgtccta
      661 tctatccact gcaacatagc aactttccca ataccagtgt tccagaattt tttatcataa
      721 ataccaattc ccaattccat ccatcttgtt tgtttacata cccaataacg cgaaacagtc
      781 ccaactagtt tatcatcaac aaaaatacca aggcgatttg agttgcttaa aatggattct
      841 gatttttgta gttcgaattc tttaaaattt gaaaaatact gataatcatc ataataggga
      901 gcatcatact gtttccaaac tggattaagt tgtgaataag ccatttccca taaagacatc
      961 aaatcttctc tctttaactt tcttaaataa atcatactat tcccctaatc caaggccttg
     1021 acttccaaca tcatatcacg gatctgagcc gccagttcaa agtcaagcac ttcgacggct
     1081 tcttgcattt gtttttctag tttcttgacg agttctttgc gctcttgttt gttgaggcta
     1141 ttgatgtcga cttccttgtc ctcttcctta gcaactgcct tggtcacggc aatcaggtca
     1201 cggatttctt tcttgattgt ctgtggtacg ataccatgct cttcattata ggccatctga
     1261 attttccgac ggcgggcagt ttcatcgata gcacgttgca tagactgggt aaccgtgtct
     1321 gcatacatga tgacatggcc ttcactgtta cgagcagcac gtccaatagt ctggatgagt
     1381 ccacgttcgt tgcgaaggaa accttccttg tcagcatcga gaatagctac gaggctcact
     1441 tcaggaacgt caattccttc acggagcagg ttaattccga ccaagacatc aaagacaccc
     1501 aagcgcaggt cacggataat ctccgtccgt tccaaggtct tgatatccga gtgcatgtac
     1561 ttgaccttga tacccatttc cttgaagtag tcggtcaaat cctctgccat tttcttggtc
     1621 aaagttgtga taaaggtacg ctcatttttt tcaacgcggg cattgatttc acccaagagg
     1681 tcatcaatct gtcccatagt cggacggact tccacctctg gatccaagag tcccgttgga
     1741 cgaatgattt gctcaatcac tgtctcggtc tgttcatttt catagtcacc aggtgtcgct
     1801 gaaacgtaaa caatctgatg aacgtgactc tcaaactcct cccgacggag aggacgattg
     1861 tccaaagcag acggcaaacg gaaaccataa ttaaccagca tttctttacg cgaacggtct
     1921 ccattgtaca tgcccttgat ttgccctatg gtcatatgac tctcgtcaat cataatcaag
     1981 aaatcatctg ggaagaagtc gagaagcgta taaggaggct ctccttcgct ccgtccatcc
     2041 atgtggcgag aataattttc aaccccattg gtatagccca tctcacgcaa catttcgata
     2101 tcatactctg tccgctgttt caaacgctgg gcttcaagca gtttaccttc cttttcaaag
     2161 acagctaatt gttcttccaa ctcggcctga atctttgcaa tggcaacttc catgtggtcg
     2221 tcattggtca caaagtgtgt cgctgggaaa atcgctaaat gatccacttc tcccaacacc
     2281 tgacctgtca gagcctcaac ttcacgaata cggtcaattt cgtctccaaa aaattctact
     2341 cgaaaggcat gttcatctcg ggaagctggg aaaatctcta ccacatcccc acgaacgcga
     2401 aatcttccgc gttggaaatc aatatcatta cgttcaaact gaatatcgac caagtcattc
     2461 aagagtttat cacgagaaat ctctagacca ggacggagac taacgacact atcagcgtat
     2521 tccttgggcg aacccaaacc atagatacaa gagactgagg ccacgacaat aacatcatta
     2581 cgctccaaaa gggctgaggt agctgagtgg cgaagcttgt caatctcgtc attgacagaa
     2641 ctatccttct caatataggt atcgctagaa gggacatagg cctctggctg gtaataatca
     2701 tagtaggata cgaaatactc aactgcattt tcagggaaaa attccttaaa ctccccatag
     2761 agctgaccag ccagagtttt attgtgggca ataaccagag ttggtttatt gactttagaa
     2821 atgacctgac tcatagtata ggtcttccct gttccagtcg cccccatcag aatctgagct
     2881 ttttctcccc cctcaatgtt attcaccaac tgctcgatag cttggggttg atctcctgat
     2941 ggttgatatt ttgatactag tttaaattga ttatctgtaa tgtgattgat cataagaatc
     3001 ctctctccat gtgctattcc tattttagca tacttatagt attttcacga agaaaaggac
     3061 ggaccgaagt cacatccttt cattttcttt ctttaattta ttagcaagat agacaatcag
     3121 cagtagtaat accagacttg tcataatgga accttctatg ccaaaagaac cacctgatag
     3181 ccaatcttga ttgccttgtg gtaaaaaggt catcagagac gttcctgacg gttgaccact
     3241 aactaaaatc ccaaagagat taccctgagc aaaattccaa gcaccatgaa tacctgcaac
     3301 accccaaact gtatcagttt tgagaaggta aagagccatg gcaactccga ataaaaagag
     3361 atttactaga gatagagggg tgagaccaga attgcccata tgaagcaggg taaagaacag
     3421 gctagatata agaatagcta gttttagatt ggttcttgag gccaattgag gaagtagcca
     3481 agcacgggcc accacttctt ctgctgtccc ctgtaaaatc caaaatggga tagtaaagac
     3541 gacaaaggca agagaataag gattcaagtg aatggattcc aaacgatatt gacctaagac
     3601 cactaaacct aacaaggtca gaagaaaaag tgccaggcct agactaaatc ctttcagaag
     3661 attgctgagg aaattctctc tataaaatcc caaggttcga ataggtcttt tctcaacttt
     3721 tcttgtccat ctgaacacag tgttaagaat aaaaccaaag gccagtaatc ctaaaattaa
     3781 acgaaagtca cttgttttat ccgtcaagct ctgatgcaag gtctgcaagt agcttgcaag
     3841 attttgacca gcctctccaa aatttctagc aagtccgatg agagctaaca agccaatacc
     3901 atacaaagta aaagccacaa actctcctat gaagacaaaa acaaaggcta acaaggggct
     3961 tgtgtaaatg tttaaactca tttttgaagc ttggaagtct ttaaatattc ttttgttctt
     4021 catatccccg tcctcttttc ttctatatat actattatta tagcactttt taatagcaat
     4081 tcaaggaaag gaaaaaatcg atgttatatt ttctgacacg aaaaatataa aaatttgcct
     4141 ttttttactt tttctgatat aatgagagaa tattcggaaa aggagactaa aaatgaagaa
     4201 aaaatttcta gcatttttgc taattttatt cccaattttc tcattaggta ttgccaaagc
     4261 agaaacgatt aagattgttt ctgataccgc ctatgcacct tttgagttta aagattcaga
     4321 tcaaacttat aaaggaattg atgttgacat tattaacaaa gtcgctgaga ttaaaggctg
     4381 gaacattcag atgtcctatc ctggatttga cgcagcagtc aatgcggttc aagctgggca
     4441 agccgacgct atcatggcag ggatgacaaa gactaaagaa cgtgaaaaag tcttcaccat
     4501 gtctgatact tactatgata caaaagttgt cattgctact acaaagtcac acaaaattag
     4561 caagtacgac caattaactg gcaaaaccgt tggtgttaaa aacggaactg ccgctcaacg
     4621 tttccttgaa acaatcaaag ataaatacgg ctttactatt aaaacatttg acactggtga
     4681 tttaatgaac aacagcttga gtgctggtgc catcgatgcc atgatggatg acaaacctgt
     4741 tatcgaatat gccattaacc aaggtcaaga cctccatatt gaaatggatg gtgaagctgt
     4801 aggaagtttt gctttcggtg tgaaaaaagg aagtaaatac gagcacctgg ttactgaatt
     4861 taaccaagcc ttggctgaaa tgaaaaaaga tggtagtctt gataaaatta tcaagaaatg
     4921 gactgcttca tcatcttcag cagtgccaac tacaactact ctcgcaggat taaaagctat
     4981 tcctgttaag gctaaatata tcattgccag cgattcttct tttgcacctt ttgttttcca
     5041 aaattcaagc aaccaataca ctggtattga tatggaattg attaaggcaa tcgctaaaga
     5101 ccaaggtttt gaaattgaaa tcaccaaccc tggttttgat gctgctatca gtgctgtcca
     5161 agctggtcaa gccgatggta tcatcgctgg tatgtctgtc acagatgctc gtaaggcaac
     5221 ttttgacttc tcagaatcat actacactgc taataccatt cttggtgtca aagaatcaag
     5281 caatattgct tcttatgaag atctaaaagg aaagacagtc ggtgttaaaa acggaactgc
     5341 ttctcaaacc ttcctaacag aaaatcaaag caaatacggc tacaaaatca aaacctttgc
     5401 tgatggttct tcaatgtatg acagtttaaa cactggtgcc attgatgccg ttatggatga
     5461 tgaacctgtt ctcaaatatt ctatcagcca aggtcaaaaa ttgaaaactc caatctctgg
     5521 aactccaatc ggtgaaacag cctttgccgt taaaaaagga gcaaatccag aactgattga
     5581 aatgttcaac aacggacttg caaaccttaa agcaaacggt gaattccaaa agattcttga
     5641 caaataccta gctagcgaat cttcaactgc ttcaacaagt actgttgacg aaacaacgct
     5701 ctggggcttg cttcaaaaca actacaaaca actccttagc ggtcttggta tcactcttgc
     5761 tctagctctt atctcatttg ctattgccat tgtcatcgga attatcttcg gtatgtttag
     5821 cgttagccca tacaaatctc ttcgcgtcat ctctgagatt ttcgttgacg ttattcgtgg
     5881 tattccattg atgattcttg cagccttcat cttctgggga attccaaact tcatcgagtc
     5941 tatcacaggc caacaaagcc caattaacga ctttgtagct ggaaccattg ccctctcact
     6001 caatgcggct gcttatatcg ctgaaatcgt tcgtggtggt attcaggccg ttccagttgg
     6061 ccaaatggaa gccagccgaa gcttgggtat ctcttatgga aaaaccatgc gtaagattat
     6121 cttgccacaa gcaactaaat tgatgttgcc aaactttgtc aaccaattcg ttatcgctct
     6181 taaagataca actatcgtat ctgctatcgg tttggttgaa ctcttccaaa ctggtaagat
     6241 tatcattgct cgtaactacc aaagtttcaa gatgtatgca atccttgcta tcttctatct
     6301 tgtaattatc acacttttga ctagactagc gaaacgctta gaaaagagga ttcgttaatg
     6361 gcaaaattaa aaattgatgt aaatgattta cacaagcact atggaaaaaa tgaagtccta
     6421 aaaggaatta cgactaagtt ctatgaagga gatgttgttt gtatcatcgg tccttcaggt
     6481 tctggtaagt caactttcct ccgtagcctc aatcttttag aagaagtcac tagcggtcac
     6541 atcactgtga acggctatga tttaactgaa aaaacaacca atgttgacca cgtccgtgaa
     6601 aatatcggca tggtattcca acacttcaac ctcttccctc atatgtctgt attggacaac
     6661 atcacctttg ctcctattga gcacaagttg atgactaagg aagaagctga ggaattggga
     6721 atggagttgc ttgaaaaggt tggactagca gataaagcta atgccaatcc agatagccta
     6781 tcaggtggtc aaaaacaacg tgtggccatc gctcgtggcc tagcaatgaa tccagacatc
     6841 atgctcttcg atgaaccaac ttctgccctt gaccctgaga tggttggaga cgtacttaac
     6901 gttatgaagg aattggctga gcaaggcatg accatgatta tcgtaaccca tgagatggga
     6961 tttgctcgtc aggttgccaa ccgcgttatc tttactgcag atggcgagtt ccttgaagac
     7021 ggaacacctg accaaatctt tgataaccca caacaccctc gtctgaaaga gttcttagat
     7081 aaggtcttaa acgtctaaac tcaaactgta aggatttcct tgcagttttt ctacctcgta
     7141 ttggaatttt tgatttttcg gaaaattatg ttagaattaa gtttatgaaa tgaggtttcc
     7201 tcatacctag caagactagg aataaaaata gaaattaggt agctagatgt catctaaggt
     7261 tattgttaca attttcggtg cgagtggaga cctggctaaa cgcaagctct acccttccct
     7321 ttttagacta tatcaatccg gcaatctttc caagcacttt gccgttattg gaactgcccg
     7381 tagaccttgg agtaaggaat attttgaatc tgtagttgtc gagtccatcc ttgatttggc
     7441 agatagtacc gagcaagccc aagaatttgc tagccacttc tactatcaaa gccatgatgt
     7501 caatgattcg gaacattata ttgctttgcg tcaattacaa gctgagctta atgaaaaata
     7561 ccaagctgaa cacaataagc tcttcttctt gtctatggca cctcagttct ttggaaccat
     7621 tgccaaacac ctcaaatctg aaaacattgt cgatggcaaa ggttttgagc gcttgatcgt
     7681 tgaaaaacca tttggtacag attacgcaac tgcaagcaag ttgaatgacg aactcctagc
     7741 aacatttgac gaagaacaaa ttttccgtat cgaccattat cttggtaagg aaatgatcca
     7801 aagcatcttt gcagttcgct ttgcaaactt gatttttgaa aacgtttgga acaaggattt
     7861 tatcgacaat gttcaaatta cctttgcgga gcgcttgggt gtagaagaac gtggtggcta
     7921 ctatgaccaa tccggtgccc tccgtgacat ggtccaaaac cacactctac aacttctttc
     7981 gctcctcgcc atggacaaac cagcaagctt cacaaaagac gagattcgtg ctgaaaagat
     8041 taaggtcttt aaaaacctct atcatccaac tgatgaagaa ctcaaagaac actttatccg
     8101 tgggcaatac cgctctggta agattgatgg catgaaatac atctcttatc gtagcgagcc
     8161 aaatgtgaat ccagaatcaa caactgaaac ctttacatct ggtgccttct ttgtagacag
     8221 cgatcgattc cgtggtgttc ctttcttttt ccgtacaggt aaacgactga ctgaaaaagg
     8281 aactcatgtc aacatcgtct ttaaacaaat ggattctatc tttggagaac cacttgctcc
     8341 aaatattttg accatctata ttcaaccaac agaaggcttc tctcttagcc taaatgggaa
     8401 gcaagtagga gaagaattta acttggctcc taactcactt gattaccgta cagatgcgac
     8461 tgcaactggt gcttctccag aaccatacga aaaattgatt tatgatgtcc taaataacaa
     8521 ctcaactaac tttagccact gggatgaagt ttgtgcgtca tggaagttga ttgaccgtat
     8581 tgaaaagctc tgggctgaaa atggtgcccc acttcatgac tataaagctg gaagcatggg
     8641 acctcaagcc agctttgacc tacttgaaaa attcggtgcc aaatggactt ggcaaccaga
     8701 tatcacctat cgtcaagatg gtcgcttaga ataaaaaaat ttcctgcaag tttatgcctt
     8761 gcaggatttt ttagcttctg attagattaa accttccaag agacctttca tgaagttttc
     8821 tgagttaaac tctccaatat catcgatttt ttcaccaaaa ccaatcaatt ttacaggaat
     8881 attgagttct tcacgaatgg ctagaaccac acctcctcga gcagttccat caatcttagt
     8941 caaaacaatt cccgttaaag gtgtgatttt cgaaaattct ttggcctgta ctagggcatt
     9001 ttgacctgtt gatgcatcaa gtgccaagaa ggtttcatgt ggtgcttctg gcacaacacg
     9061 tttgataata cgaccaatct tttccaactc agccataagg ttatccttat tttgcagacg
     9121 accagcagta tcaatcatga gaatatcgat accttcagcc acggcacgtt ccataccatc
     9181 aaagaccacg ctggctggat cagctttttc aggtccagtt actactggaa catctactcg
     9241 tcggccccat tcagctagct gagctactgc acccgcacgg aaggtatctg ctgcaaccag
     9301 catgaccttc ttaccagctc gtttgtagcg gtgggctagt tttccgatag aagttgtttt
     9361 cccaacacca ttcacaccaa caaagagcat aactgtcaag ttatcttgga agtggatgct
     9421 ttcatcgtag ctaccatcct tttcataaag ctcaaccaat ttctcaatga tgacacgacg
     9481 aagtgcatca ggtttcttgg cattttcaag cttggcttcg taacgtagtt cctccgttaa
     9541 gttagaagcg acttggacac caacatcact cataatcaac agttcttcca gttcctcgaa
     9601 aaattcttcg tcaacagagc ggaagttagc aaagaaggca ttcaagcggg caccgaaacc
     9661 tgtgcgagtt ttcttaagac tgcggtcata tttttcctga acagtttctt ctgtttgagg
     9721 cctttctgct tcaagaattt cagaattatt ttcttctata gttccttcgt gctcaagctt
     9781 ctcttcctct ggtaattctt ctgagtttgg taattcttct atttcttctt gagaaacccc
     9841 tacagctggc tctgaatcct gactttcttc aactgtgtct tgactatcct cttcttggaa
     9901 cacagattct tcagaacttt caacctctgc ttcttcttga gaaacttcct cagcttctgt
     9961 gaaggcaggc tcaatatctt cagacaaatc aagattttcc agagcttctt ttacaacttc
    10021 ttcgatttta ggttcttctt tttttccgaa tagacggtca aataatccca tatcttagtt
    10081 ctcctttagc acatattctt cgatagccca ggcgacagct tcttcatcat tggtcatagg
    10141 agttaccaca tttgcgactg ccttgacttc aggaacagcg ttttgcatgg caacaccgag
    10201 ccctgcccat tcaatcatag agaggtcatt ggcctcgtca ccacaggcca tcacttgact
    10261 ttgatcaatt ccaagatggc taattagttt tgccaaacct gttgctttat gaacattctt
    10321 tggtgaccat tctagcaaca tttcacgtga tttaaagatt tcatattggt caaacaattc
    10381 tggagaaatc ttctgaatgg ctgcatccaa gggttcttga gcaaaggcgg tcacgcattt
    10441 gttgtaggtc atttgattag acaggtcttc aaaatcaact ggaacaaaag tcaaagctgg
    10501 attgaatttg gcataaagac tttcttggtc cgattgaatt tgataaacag ttccttctga
    10561 gatagcgtca agaggcagtg ataatttctc tgtttcttca tacaagcgtg ccacatcatc
    10621 atatgaaaag actgtcttat caaggatttc acctgtattt ttctgaacca aaccaccatt
    10681 aaaagtaatg gtatactcat cttcctgacc gtcagtccct agctcatgga gaaagaaatc
    10741 catagctttt aagggacgac cagttgtcaa tacgaccttg ataccacgat cacgcgcagc
    10801 tttcaaggtt tccttggtac gatccgtcag ccttttatca gtagtcagca aggtcccgtc
    10861 caagtccaat gcaatcaatt ttatatctgc cattataagc cctccataca agctataacc
    10921 gactggtcct tatggtgacc aatcacagtc tttgctaatt ctaaaatttc aggtcgtgca
    10981 ttttcaggag ctacaggatg tcccacaacc tgcatcatat gtaagtcatt aagattgtct
    11041 ccaaaagcca tgacctgatc cattgtgata ccaagttttt taactaattc aacaatggcc
    11101 actcccttat cgacatagtc cagaacaata tcaatagatt caaagccagt tgtcatggcc
    11161 ttaacaccag ggacattatc attgacccaa gcttccccag cttccagcgt ttcttctgtg
    11221 aagttggttg taaatttgaa aatgtcatct gtgatatctt ccaaactcgc tactttttgg
    11281 atattttcat tatagtgctg actcactttc aaataggtct catcaaccgt atctagaaca
    11341 tatgaaccct tcttacccgt caagagcagt ttattgatat ctacataagg tgaagttttc
    11401 agcttttcaa aagttgccag ataaaagtca cgagacatag tcgcttcata caagtcctga
    11461 ccttgatact ctaccaaact gccattttcc gcgatgaaaa taatgtcatc acgaacacca
    11521 gcaaataatt tttctagaga cagaaatccc cgacccgaag ctaccgcaaa gtaaatccct
    11581 ttttccttgt aggaaaccaa gagagacttg agacgatcca tatcaaagcg tccattccca
    11641 tctaggaagg ttccgtccat atccgttgct actagtttaa ttgtcatcct tcaatacttt
    11701 ctaaatcttt taacttaact gaaacaatct ttgaaacacc cgattcttgc atggtcactc
    11761 catagatgga atcagccgct gccatggttc ccttacggtg ggttacgacg atgaactggc
    11821 tgtccttgtc aaagcggttg aggtaatccc caaaacgttt aacattggct tcatccagcg
    11881 cagcttccac ctcatccaag ataacaaatg gaatagtctt gacacgaata atggagaaga
    11941 gcaaggcaag agccgatagg gctttttcac caccactcat gagattaaga gactggattt
    12001 tcttgcctgg tggttggaca gaaatttcaa ccccagctgt cagcaagtct ccttcagtca
    12061 aaatgaggtc agcctgacct ccgccaaaca tctgcttgaa ggtcacttta aaggactcac
    12121 gaatagcttc aaaggttgat ttaaagcgtt ccttgacctc atcattcatt tctgtaatgg
    12181 tctcaaggag cagatttttc gctgacaaaa tatcatctcg ctgactattt agaaattcca
    12241 gacggctgtg aacttcttcg tactggtcaa tagcttccaa gttgacaggt cccagtgagc
    12301 gaatagcctt ctctaaatcc tgcacctctt gctctgccag attgagattt tccaactcat
    12361 gcgctttttc tagtgcttca gtatagctaa tctggtactg gtctgttaat tgattttgta
    12421 gatggcgcaa gcgctcgctg accttttctt tcttggcttc agcacgtgtt tgcttgcgaa
    12481 tccactcctc attctgctgg cgagcctgat ccaaatgact agcaatatca tccagttgac
    12541 cctcaatatc atccaactca aactgcttgc gaatcaaacc ttgttggaga tttgtttttt
    12601 gagttttggc ttcatcagcc tgttgactga gcaaatccgt atcaactttc tcaagattgt
    12661 caaccttttc ttgaagaagg cgctgaattt cttcttgctc aatatcaaga ttatctagtt
    12721 ccttacctaa gcgttcaata tcagcaactt cataacgttt ttgcccttgc agttctgtct
    12781 taagtagacg agcttgagct agctcttcct gcaagttttg ataacgttct tggatggcgt
    12841 ttttattaga cttaatctct tcaatctcag cttccagatt attcttttca ctagtgattg
    12901 tagtaaggcg ctcttggcat ttttccttat ccgcttgcca ttctccctca gaaagacgat
    12961 ttaattcctc ttcttggagt ttccaaagag tttctagctc ttcaacttgc tgactggtct
    13021 gctgataggc gagggacaag ccttgctcct gaatacgagc ctgctctccc tgagatttga
    13081 tggcttctaa tctttctgtc aatctagcta gctcatcttg caagttcttc aaactcactt
    13141 catctgaacg caaacttgct tcttcttcag caatttcttt ttgtaattgc tccagttctg
    13201 gcttgatgaa aatactgtta ttctggcgat tagctccacc tgcataagaa ccacctgtgc
    13261 gcagctctgt tccgtccaat gtcaccatac gaacctgata acgaacctgg cgcgctgctg
    13321 cacgcgcatg ctccacggta tcaaaaatgg ccgttgtagc tagcaaattc ttgaaaatgg
    13381 cttccagtct agtatcgaaa gtaaccagct catctgccat gcccagaaat cctggacttg
    13441 cagcaatagt atcttggttc tgactagaaa tcgtacgagc cttgatagtg gtcaaaggaa
    13501 ggaaggttgc acgaccagat ctgtttcgtt tgagtaaatc aatcgccttg gttgccgact
    13561 cttcatcttc cacgatgata tgttggctac tcgctccaag cgcaatctct agggcagttt
    13621 gataatgcac atcaaaggtc aggtgctcac taactgctcc aataatccca cctagacgat
    13681 ctttctcttg gagaacactc ttaacacctg cataaaagtt actatgattt ctcaggataa
    13741 tttccaaact ttgcgctcta gcctgcttgt ttttgagact atccagacgg tcaaagagtt
    13801 gactttgttg agcttgatag gaagttttct gctcctcttg ctccttagca cagacttgat
    13861 agtcggccaa taatttctga acctgctcct tggcagtttc aagctcagcc ttttgctgac
    13921 cagccttctc tttagctgta gccaactgtt ctttcagctt ttctagttga tctgcttgtt
    13981 tttgagaaag ctgacgacta ttttccaact cattctcaat acgggtcagt tggtttgaga
    14041 catctgcttc ttcttgtaaa agggccacaa agcgttcacg taagagctca atcatctgat
    14101 caggatcatc tgaaaaagct agcaattcag cctctaatcg attgagtttt tgattatttt
    14161 ggactagatt ttcttccaag agggctaaag agctttcttt atcagatttt tcttggctga
    14221 gtgaacttct cttatcctcc aaagtagcca aacgggcttg tgcttcctgt tgattgaggg
    14281 ctacttgctc agattccagt ttcgataggg ctaattttct ctctaagtca ctaatcagac
    14341 tggtcaaatc catcaaactg ccttggtctt tggccatttc agcctgtaaa tcttggcgtt
    14401 gctttttaag agtttgattt tcttcttcta atttttcacg cttttggtaa taactcatca
    14461 agagttcttg aacctgagcc aactcttctt ctgtcgactc tagttcagcc ttattttcct
    14521 tgatttgagc aaccagaacg tctaaataaa tagccttacg ttgtccttcc aagtctaaaa
    14581 acttacgggc attctcagct tgcttctcaa gaggcttgat ttgattatcc aactcgtaga
    14641 taatgtcctc taagcggtcc agattatcct gagtttgctg cagtttactc tcggtttctt
    14701 ttctgcgagt cttgtatttt aaaactccag cagcttcttc aaaaatagct cgtcgttcct
    14761 caggcttgga attaaaaatc tcctcaacct tcccttggga aataatagag aaggaatctc
    14821 gtcccaatcc agtatccaag aagaggtcat gaatatcacg cagacggact ttcttgccgt
    14881 caatcttgta ttcgctatct ccactacgat agatatggcg ttccaccctg atttcttgac
    14941 ctgcatcctt gataaatccg tcatgattat ccagagtcac aactacagaa gcataattga
    15001 gcggtttgcg actttctgtt ccagcaaaga tgacatccgg catcttgccc ccacggagac
    15061 tcttgacact agactccccc aaagcccaac gcagactttc tgtaatattg gactttccag
    15121 atccattggg tccaacaact gccgtcacac cttggtcaaa aacgaccttg gtcttatcag
    15181 caaaagactt gaacccctga atttcgattt cctttaaata catgaatcca gccccttctc
    15241 aacggcattt ttggcagctt cctgctctgc taatttctta gaacgacctt ggccttgacc
    15301 gatactctta ccttcaacaa gaacttctac atcaaagacc ttatcgtgag caggccctgt
    15361 ttcagaaatc acctgataac gaatagccac atcaccattg acctgaagta actcttggag
    15421 atgagtttta tagtctgtaa tcatctcaaa ttcgcctgct tcaaccttag gaatcatgac
    15481 ttgatagata aattccttga ccttggccac atccttatcc aaaagaaggg caccaagaaa
    15541 ggcttcaaag gcatcaccaa gaatggtgtc acgattgcga ccaccagatt tttcttcccc
    15601 tttacccaac ttgataaact ggtcaaactg gcaatcacgc gcaaaaccag ctaaactctc
    15661 ctcacggaca atcatagcac ggagttttga taggtcacct tcaggctttt taggatattt
    15721 tttatacaga tattctgaaa tcaataactg tagaacagcg tctcctaaaa attccaagcg
    15781 ttcattatgt gaaattttta agaggcggtg ctcattggca taactcgtat gagtaaaggc
    15841 agtttccagt aactttttgt ctgtaaattc gattgcaaaa tggttcttta gtacagtttg
    15901 taattctttc ataccaacct ctttctaact gataacagtc ctttttatta tatcaaaaaa
    15961 agccccctga gtcactctaa aacgggactg gaaagcattt ggaaattctt tagacagaga
    16021 ttctcagttt tagcggtaaa tttagagcag tgtaaagaaa aaagccctat taaaggcttt
    16081 ttagaatgtt tacatccacc ctgagggaat cgaaccccca tctcaagaac cggaatctta
    16141 cgtgatatcc attacactaa gggtggaaac ttgttttatt ataacagaaa tttgctctaa
    16201 taacaagttt tttggtcaaa gaccccgtct tagtgggaag catccccatt ccagatggag
    16261 tttttcacga tcacataatc aacgtgttta aggtcagcaa cctgacgtcc acctgcataa
    16321 gaaatagcac tttgaaggtc ttgttccatc tcagttaaag tgtcttgcag atgaccttta
    16381 gcaggaagca agatacgttt gccttccaca tttttgtaag cacctttttg atattgtgag
    16441 gctgaaccat aatattcttt gaactgttca ccatcgactt caatcgtttt ccctggactt
    16501 tcaatatgtc ctgcaaagag ggaaccaatc atgatcatgc tagcaccgaa gcggatagac
    16561 ttggcaatat caccgtgagt acgaattcct ccatcagcga taatcggttt acgcgcaacc
    16621 ttggcacacc agcgtagagc agccaactgc caaccacctg taccaaaacc agtcttaacc
    16681 ttggtgatac aaaccttacc aggaccgatt ccgaccttag tagcatctgc accagcattt
    16741 tccaattcac gcacagcttc tggtgttccc acatttccag caatgacaaa ggtatctggc
    16801 aattctttct tgatgtgttg aatcatagaa atcacgctat ccgcatgacc atgagcaata
    16861 tcaatagtga tatactcagg agcatcagcc ttgagctggc taacaaaatc atactcataa
    16921 tccttaacac cgacagagat agaagcaatg agcccttgat cgtgcattcg tttaataaaa
    16981 ggaatgcgtc ctacctcatc aaaacggtgc ataatgtaga agtaaccacc tttagccagt
    17041 tgctctgcta cattttcatc caaaatcgtc tgcatattcg ctggcacaac aggtagttta
    17101 aaggtgtgat ttcctaaagt gacacttgta tccgcttctg cacggctttt aatgacacat
    17161 ttatttggaa tcaattgaat atcttcgtaa tcaaaaattg gaaattcatt taacatatcg
    17221 atgtctcgtt tcttttgtaa tgacctacct atgctcttgc atcactacgc cttttccgac
    17281 gtttccttaa tttattataa accaaagtac ag
//
LOCUS       DAVMEF010000028        17278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMEF010000028 DAVMEF010000000
VERSION     DAVMEF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.028"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            91..5394
                     /locus_tag="VYQ30_000667"
     CDS             91..5394
                     /locus_tag="VYQ30_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1965824.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGEQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEATEITGLRYVPRG
                     SGLNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLGLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPIVEKPEFKLNSLASEQGKTPDYKQEIA
                     RPETPAQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5561..7720)
                     /locus_tag="VYQ30_000668"
     CDS             complement(5561..7720)
                     /locus_tag="VYQ30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1965825.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNSARSTWNSPAPQQPPSTESSISSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VYQ30_000669"
     CDS             complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VYQ30_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1965826.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKRQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8379..8906
                     /locus_tag="VYQ30_000670"
     CDS             8379..8906
                     /locus_tag="VYQ30_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1965827.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQENYFIKRL
                     TFDELNELAENFSEN"
     gene            8976..9305
                     /gene="gpsB"
                     /locus_tag="VYQ30_000671"
     CDS             8976..9305
                     /gene="gpsB"
                     /locus_tag="VYQ30_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1965828.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9326..9706
                     /gene="rnpB"
                     /locus_tag="VYQ30_000672"
     ncRNA           9326..9706
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYQ30_000672"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9791..10948
                     /locus_tag="VYQ30_000673"
     CDS             9791..10948
                     /locus_tag="VYQ30_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1965829.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10961..12355
                     /gene="mapZ"
                     /locus_tag="VYQ30_000674"
     CDS             10961..12355
                     /gene="mapZ"
                     /locus_tag="VYQ30_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1965830.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEESEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTALALVSVIICVSAYYVYRQVARSTKEIETSQSTTANRSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKSDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12431..13855
                     /gene="gndA"
                     /locus_tag="VYQ30_000675"
     CDS             12431..13855
                     /gene="gndA"
                     /locus_tag="VYQ30_000675"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1965831.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13867..14556
                     /locus_tag="VYQ30_000676"
     CDS             13867..14556
                     /locus_tag="VYQ30_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1965832.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTIQE"
     gene            14654..15610
                     /gene="cbpC"
                     /locus_tag="VYQ30_000677"
     CDS             14654..15610
                     /gene="cbpC"
                     /locus_tag="VYQ30_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1965833.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEISGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLNTSN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(15728..16960)
                     /locus_tag="VYQ30_000678"
     CDS             complement(15728..16960)
                     /locus_tag="VYQ30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1965834.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTADILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(16971..>17278)
                     /locus_tag="VYQ30_000679"
     CDS             complement(16971..>17278)
                     /locus_tag="VYQ30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1965835.1"
                     /translation="CDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKL
                     LMKKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
BASE COUNT         5309 a         3160 c         3989 g         4820 t
ORIGIN      
        1 cagggttaag cattgcactt tcagaggaag gagtattttt taaaaaggaa atgtaaacgc
       61 ttgctcaaaa atgaaaggat ttagaagttt atgaataaag gattatttga aaaacgttgt
      121 aaatatagta ttcggaaatt ttcattaggt gttgcttctg ttatgattgg agctgcattc
      181 tttgggacaa gtccggttct tgcagatagc gtgcagtctg gttccacggc gaacttacca
      241 gctgatttag ctactgctct tgcaacagca aaagagaatg atgggcgtga ttttgaagcg
      301 cctaaggtgg gagaagacca aggttctcca gaagttacag atggacctaa gacagaagaa
      361 gaactattag cacttgaaaa agaaaaaccg gctgaagaaa aaccaaaaga ggataaacct
      421 gcagctgcta aacctgaaac gcctaagacg gtaacccctg aatggcaaac ggtagagaaa
      481 aaagaacaac agggaacagt cactatccga gaagaaaaag gtgtccgcta caaccaacta
      541 tcctcaactg ctcaaaatga taacgcaggt aaaccagctc tgtttgaaaa gaagggcttg
      601 accgttgatg ccaatggaaa tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag
      661 ggcaaatcac gctttggtgt cttcctgaaa tttaaagata ccaagaacaa tgtttttgtc
      721 ggttatgaca aggatggttg gttctgggaa tacaaaactc caggtaatag cacatggtat
      781 aaaggcaacc gtgttgctgc tcctgaaaca ggatcaataa accgtctttc tatcactctc
      841 aagtcagacg gtcagctaaa tgccagcaat aacgatgtca atctctttga cacagtgact
      901 ctaccagctg cggtcaatga ccatcttaaa aatgagaaga agattcttct caaggcgggc
      961 tcttatgacg atgagcgaac agttgttagc gttaaaacgg ataaccaaga gggggtaaaa
     1021 acagaggata cccctgctga aaaagaaaca ggtcctgaag ttgatgatag caaggtgact
     1081 tatgacacga ttcagtctaa ggtcctcaaa gcagtgattg accaagcctt ccctcgtgtc
     1141 aaggaataca ccttgaatgg acatactttg ccaggacagg tgcaacagtt caaccaagtc
     1201 tttatcaata accaccgaat cacccctgaa gtcacttata agaaaatcaa tgagacaaca
     1261 gcagagtact tgatgaagct tcgcgatgat gctcacttaa tcaatgcgga aatgacagta
     1321 cgcttgcaag ttgtggacaa tcaattgcac tttgatgtga ccaagattgt caaccacaat
     1381 caagtcactc caggtcaaaa gattgatgac gaaagaaaac tactttcttc tattagtttc
     1441 ctcggcaatg ctttagtctc tgtttctagt gatcaaactg gtgctaagtt tgatggggca
     1501 accatgtcaa acaatacgca tgtcagcgga gatgatcata tcgatgtaac caatccaatg
     1561 aaggatttgg ctaagggtta catgtatgga tttgtttcta cagataagct tgctgctggt
     1621 gtttggagta actctcaaaa cagctatggt ggtggttcga atgactggac tcgtttgaca
     1681 gcttataaag aaacagtcgg aaatgccaac tatgtaggaa tccacagctc tgaatggcaa
     1741 tgggaaaaag cttataaggg cattgttttc ccagaataca cgaaggaact tccaagtgct
     1801 aaggttgtta tcactgaaga tgccaatgca gacaagaaag ttgattggca agatggtgcc
     1861 attgcttatc gtagcattat gaacaatcct caaggttggg aaaaagttaa ggatatcaca
     1921 gcttaccgta tcgcgatgaa ctttggttct caagcacaaa acccattcct tatgaccttg
     1981 gatggtatca agaaaatcaa tctccataca gatggtcttg ggcaaggtgt tctccttaaa
     2041 ggatatggta gcgaaggcca tgactctggt cacttgaact atgctgatat tggtaagcgt
     2101 atcggtggtg tcgaagactt caagacccta attgagaagg ctaagaaata tggagctcat
     2161 ctaggtatcc acgttaacgc ttcagaaact tatcctgagt ctaaatactt caatgaaaaa
     2221 attctccgta agaatccaga tggaagctat agctatggtt ggaactggct agatcaaggt
     2281 atcaacattg atgctgccta tgacctagct catggtcgtt tggcacgttg ggaagatttg
     2341 aagaaaaaac ttggtgacgg tctcgacttt atctatgtgg acgtttgggg taatggtcaa
     2401 tcaggtgata acggtgcctg ggctacccac gttcttgcta aagaaattaa caaacaaggc
     2461 tggcgctttg cgatcgagtg gggccatggt ggtgagtacg actctacctt ccatcactgg
     2521 gcagctgact tgacctacgg tggctacacc aataaaggta tcaacagtgc catcacccgc
     2581 tttatccgta accaccaaaa agatgcttgg gtaggggact acagaagtta tggtggtgca
     2641 gccaactatc cactgctagg tggctacagc atgaaagact ttgaaggctg gcaaggaaga
     2701 agtgactaca atggctatgt gactaacctc tttgctcatg acgtcatgac taagtacttc
     2761 caacacttca ctgtaagtaa atgggaaaat ggtacaccgg tgactatgac cgataacggt
     2821 agcacctata aatggactcc agaaatgcga gtggagttgg tagatgctga caataataaa
     2881 gtagttgtaa ctcgtaagtc aaatgatgtc aatagcccac aatatcgcga acgtacagta
     2941 acgctcaacg gacgtgtcat ccaagatggt tcagcttact tgactccttg gaactgggat
     3001 gcaaatggta agaaactttc tactgagaag gaaaagatgt actacttcaa tacgcaggcc
     3061 ggtacaacaa cttggaccct tccaagcgat tgggcaaaga gcaaggttta cctttacaag
     3121 ctaactgacc aaggtaagac agaagagcaa gaactaactg taaaagatgg taaaattacc
     3181 ctagatcttc tagcaaatca accatacgtt ctctatcgtt cgaaacaaac caatcctgaa
     3241 atgtcatgga gtgaaggcat gcacatctat gaccaaggat ttaatagcgg taccttgaaa
     3301 cattggacca tttcaggcga tgcttctaag gcagaaattg tcaagtctca aggggcaaac
     3361 gatatgcttc gtattcaagg aaacaaagaa aaagttagtc tcactcagaa attaactggc
     3421 ttgaaaccaa ataccaagta tgccgtttat gtcggtgtcg ataaccgtag taatgccaag
     3481 gcgagcatca ctgtaaacac tggtgaaaaa gaagtgacta cttataccaa taagtctctc
     3541 gcgctcaact atgttaaggc ctacgcccac aatacacgtc gtgacaatgc tacagttgac
     3601 gatacaagtt acttccaaaa catgtacgcc ttctttacaa ctggatcgga cgtctcaaat
     3661 gtaactctga cattgagtcg tgaagctggt gaacaagcaa cttactttga tgaaattcgt
     3721 acctttgaaa acaattcaag catgtacgga gacaagcatg atacaggtca aggaaccttc
     3781 aagcaagact ttgaaaatgt tgctcagggt atcttcccat ttgtagtggg tggtgtcgaa
     3841 ggtgtcgaag acaaccgcac tcacttgtct gaaaaacacg atccatatac acaacgtggt
     3901 tggaatggta agaaagtcga tgatgtcatc gaaggaaatt ggtcactcaa gacaaatgga
     3961 ctagtgagcc gtcgtaactt ggtttaccaa accatcccac aaaacttccg ttttgaagca
     4021 ggtaagacct accgtgtaac ctttgaatac gaagcaggtt cagacaatac ctatgctttt
     4081 gtagtcggta agggagaatt ccagtcaggt cgtcgtggca ctcaagcaag caacttggaa
     4141 atgcatgaat tgccaaatac ttggacagat tctaagaaag ccaagaaggc aacattcctt
     4201 gtgacaggtg cagaaacagg ggatacttgg gtaggtatct actcaactgg aaatgcaagt
     4261 aatactcgtg gtgattctgg tggaaatgcc aacttccgtg gttataacga cttcatgatg
     4321 gataatcttc aaatcgaaga aattacccta acaggtaaga tgttgacaga aaatgctctg
     4381 aagaactact tgccaacggt tgccatgact aactacacca aagagtctat ggatgctttg
     4441 aaagaggcgg tctttaacct cagtcaggcc gatgatgata tcagtgtgga agaagcgcgt
     4501 gcagagattg ccaagattga agccttgaag aatgctttgg ttcagaagaa aacggctttg
     4561 gtagcagaag actttgaaag tttggatgcg ccagctcaac caggtgaagg cctagagaat
     4621 gcttttgatg gcaatgtgtc tagtctatgg catacatctt ggaatggtgg agatgtaggc
     4681 aagcctgcaa ccatggtctt gaaagaagca actgaaatca ctggacttcg ttatgttccg
     4741 cgtggatcag gtttaaatgg taacttgcga gatgtgaaac ttgttgtgac agatgagtct
     4801 ggcaaggagc atacctttac tgcaactgat tggccagata acaataagcc aaaagacatt
     4861 gattttggta agacaattaa ggctaagaaa attgtcctta caggtactaa gacttacgga
     4921 gatggtggcg ataaatacca atctgcagcg gaacttatct ttactcgtcc acaggtagca
     4981 gaaacacctc ttggcttgtc aggctatgaa gcagctttgg ctaaggctca gaaattaaca
     5041 gacaaagaca atcaagagga agtagctagc gttcaggcaa gcatgaaata tgcgacggat
     5101 aaccatctct tgacggaaag aatggtggaa tactttgcag attatctcaa ccaattaaaa
     5161 gattctgcta cgaaaccaga tgctccaatt gtagagaaac ctgagtttaa acttaactct
     5221 ttagcttccg agcaaggtaa gacgccagat tataagcaag aaatagctag accagaaaca
     5281 cctgcacaaa tcttgccagc aacaggtgag agtcaatctg acacagccct cttcctagca
     5341 ggtgttagcc tagccctatc tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta
     5401 gtaaaacctc ttaacaagat tacggaagca gtctctatct tttccaatga ggtttatagt
     5461 acagaaaaag cctgagaaga tgtcttctca ggcttttgtt aagcacataa atacaatagt
     5521 gctatgacaa aatcacccag aaaaatctgg gtgataaatg ttatggttgt gctggttgag
     5581 gattctgatt ttgttgatca ggggttgtat ttgattgttg cgtattattg ttaggattgg
     5641 tagtcgtact attatttgtg cttggagtgg ttgagctaga ctgtgaagtt gaactatctg
     5701 atgatgagat tgaactttca gttgatgggg gttgttgtgg agcaggtgag ttccacgtag
     5761 aacgagcact atttttaaat acgaattctc catttctgta gagcccctct ggtatattcc
     5821 aatcctctgg attgcttcct tcagacaggt aggtcatcat agagcggtaa actttggcag
     5881 cgaccgtaag gccattgcct acaagtggtg tcagacggtt agaatagcct gtccatacag
     5941 ccattgaata tttacgcgta tagccagcaa atagttcgtc aggtgctaca aattgagagg
     6001 tcttgatgtg gttttcaatt tcctcgtctg tatagttaga ggttcctgtt ttaccagcct
     6061 gagggagcca agcaagatag gcatttcgtc cagttccata actcaagact gttttcatca
     6121 tgtcggtcat catataggct gtcgtttcct tcatggcacg agttccgaca ttagagaact
     6181 ctttttcact cccatcacta aagacgactt tatggatata cattggttta tagtaagttc
     6241 caccatttgc aaaggcagcg taagcagcag ccatcttttc actacttgct ccatattttt
     6301 tgtctgattc ggttgtgtta cttgaaatgg catttgagta gtgaatactt gggtagtcga
     6361 ttcctagacc atttaggaaa gtcttggcgc ggttgagtcc gaccttgttt agagtttcca
     6421 cggctgggac gtttcgcgat tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc
     6481 ccctatccca gttataaaca ggagtatttg tcccagggta gttatagggc tcatcgtgaa
     6541 cgatagtagc agttgaatcg tagacaccgt actccaaggc aggagcatag tctgtgatcg
     6601 gtttcatagt tgatccccag tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa
     6661 cattacttga ctgatggcgt gctcctagct gggcaatgac tttaccgtta gaaacatcaa
     6721 caatggtaga agcgacttgc aattcatcgt ctggataggc aacgtattcg tctgtattgt
     6781 aaatatccca cagatgtttt tgagcttctt ggtctacatt tgtgtagaca tccatcccag
     6841 ttgtgagtag gttatagcct gtttcttctt caacttgatt gatgacttcc ttgaggtaat
     6901 tatccatgta agcagggtaa ttacttgctg atttgagact ttgtagccca tcagtaattg
     6961 gtgtattgac tgctttctca tactgttcag cagagatgta gccttgattt ttcatttcag
     7021 ataagaccaa gtttcggcgg tcttgggctg cttctggatg tgaatagggg tcatattggt
     7081 ttggtgcctg aggcattcca gccagcaagg ctaactgagg taaacttaaa ttattgaggt
     7141 ctttaccata gtagttttga gctgctgtct gcattccata gttcccatta gacatgtaga
     7201 ccttatttat atagtaggtc aagatttctt gcttggttgc tttttgttct aactgaatcg
     7261 ctaaccaagc ttcctgagcc ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt
     7321 aagtcaactt aatcaactgt tgagtgagag ttgatccacc ttggagggaa ttgctttgca
     7381 gattgcgcaa gaaagctccc aggatacgga tggtatcaat ccccctgtgg tcgaagaagc
     7441 gatggtcttc gatagaaacg attgccttaa ccaaatctgt gggaatatca ttagcttggg
     7501 cattgacgcg gcgttcagaa cccaagtcag caatgagttg atttttattg tcgtagattt
     7561 tactagaagt tgttgcaact agtttactct cggataggct aggagccttg ctaacgtagt
     7621 agaaaaaaac tcctccgcct aagacaatgg ctgcgataac caagcttaag aagctaatgc
     7681 tcagatactt gattaggcgc agaatcgttg gtttgttcat cttgttttac cacctaataa
     7741 atgttctttg ataacattga gataaggaat ttgagggaag gcaccagcct tgatttcata
     7801 tccatattct cgaatatatt caagtggcat tgatttttgt ctcttatctt gatgatagaa
     7861 gcgaatcaaa tcgaatgccg gcaataagta ggtttcttgc tgagaagaaa agtgaagaag
     7921 gacaaagcag attccttgtt gggcaaggac ttgttccata tgctgaatct gatgtggatg
     7981 aaaatttttc atcggaatcg cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta
     8041 atatccatta taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac
     8101 aatcttggca cgacttcgtt gtggatagtc cacttgtacg atttgaatag gagttggttt
     8161 cttatgtata acagccaagc cctgagacaa atagtagtcg ttggtagcat tgatcatctt
     8221 ttcaaaagac attcctcgat ttgcgaaatt tttgggttga gaaagagatg tttgtctttt
     8281 ttgtgatgaa actttatgtg gatagttgac cataattctc cttattggta caataacatc
     8341 actttattat accataattt gcacagaaag ggttaaaaat ggctacagct ttggttttag
     8401 gctattctgc ttttgatttg ggtttatttt ctgataagga tcctcgtctt aaattgatta
     8461 aaaaagcgat acgtaaagat ttagaggcta tggcagcaga tggggtgtct tggcttgtgt
     8521 ttacagggag tttgggtttt gaatattggg ttttagaggt tgctcaggag atgaaaaccg
     8581 aatacggttt ccagttggcc accatttttg cttttgaaac tcatggggaa aattggaatg
     8641 aaggcaatca gatgaaactg agtcgcttta agcaggtaga ctttgtcaaa tatgcttatc
     8701 ctcgctatga acacaagggg cagttaagag attaccagca gtttttgctg gaaaacacga
     8761 ctagttccta tcttttttat gatgaagaaa atgaaacaaa actagcttat ttttaccaaa
     8821 agatgaaaaa tcaagagaac tattttataa agagattaac atttgatgaa ttaaatgaac
     8881 ttgctgaaaa tttttccgaa aattgaagct ttgaccttga tttttgtttg ccttttttta
     8941 tataataata ctagcaagcg agaatggaga gagacatggc aagtattatt ttttcagcga
     9001 aagatatttt tgaacaagag tttggacgtg aagtccgtgg ctataataaa gtagaagttg
     9061 acgagttttt agacgatgtc atcaaggact atgaaaccta tgctgccttg gtcaagtcac
     9121 ttcgtcagga aattgcggat ttgaaggaag aattaactcg taaaccgaaa ccttcaccag
     9181 ttcaagcaga accccttgaa gcggcaatta caagttctat gacgaatttt gatattttga
     9241 aacgcctgaa tagattggaa aaagaagttt ttggtaaaca aattttagat aactcagatt
     9301 tttaagtagt tatttgagat gtgcaatttt tggataatcg cgtgaggaga attgcttctc
     9361 atgaggaaag tccatgctag cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac
     9421 cataagccta gggacgagaa atcgttacgg cagttgaaat ggctaagtcc ttggataggc
     9481 cagagtaggc ttgaaagtgc cacagtgacg gagtctttct ggaaacagag agagtggaac
     9541 gcggtaaacc cctcaagcta gcaacccaaa ttttggtcgg ggcatggagt acgcggaaac
     9601 gaacgtagta ttctgactgc tatcagctag agctgttagt ggtagacaga tgattatcga
     9661 aggaagtggt cctagtcact tctggaacaa aacatggctt atagaaaatt gcatataggt
     9721 tggggctgag aaattttctc aacctcattt tttaaagtgg acatatagaa aggtcttgca
     9781 agactgtaac atgaaaaaag aatttaattt aattgcaact gtggcagcag ggcttgaggc
     9841 tgtcgttggt cgtgaagtgc gagagttggg ctacgattgt caggttgaaa atggacgtgt
     9901 tcgttttcaa ggagacgtga gagctattat cgaaaccaac ctttggcttc gggcagcaga
     9961 tcgtatcaaa attatcgtag gaacgttccc agctaagact tttgaagagc tatttcaggg
    10021 agttttcgct ttagattggg aaaattattt accacttgga gctcggttcc cgatttcaaa
    10081 agctaaatgt gttaagtcca aacttcacaa tgagcccagt gttcaggcta tttctaagaa
    10141 agctgttgtc aagaaattgc agaaacacta tgctcgccca gaaggggttc ctctgatgga
    10201 gaatggccca gagtttaaga ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat
    10261 tgatacgacc gggtctagcc tctttaaacg tggttatcgt accgaaaaag gtggcgctcc
    10321 tatcaaggaa aatatggcag cagccatttt acaactttct aactggtatc cagacaagcc
    10381 tttgattgat ccgacctgtg gttcggggac tttctgtatt gaggcagtta tgattgctag
    10441 aaagatggcg ccaggtcttc gtcgctcttt tgcatttgag gaatggaact ggatcagcga
    10501 tcgcttgatt caagaagtgc gcacagaagc ggctaaaaaa gtagaccgtg agcttgagct
    10561 ggatatcatg ggctgtgata ttgatgctcg catggtggaa attgctaagg ccaatgctca
    10621 ggcagctggt gttgcaggag acattacttt taagcagatg cgcgtgcagg atttacgttc
    10681 cgataaaatc aatggagtaa tcatttccaa tccgccttat ggtgaacgtt tgtcagatga
    10741 tgcaggggtg accaagctct atgctgagat ggggcaagta tttgcaccgc tgaaaacttg
    10801 gagcaaattt atcctgacta gtgatgaagc ttttgaaagc aagtatggta gccaagcaga
    10861 taagaagcgt aagttataca acggaacctt gaaagtggat ctgtatcaat attttggtca
    10921 gcgtgtcaaa cggcaagagg taaaatagaa agggatactc atgagtaaaa aaagacgaaa
    10981 tcgtcataaa aaagaaggtc aagaaccgca atttgatttt gatgaagcaa aagagctaac
    11041 agttggtcaa gctattcgta aaaatgaaga agtggaagca ggagtcttgc ctgaggattc
    11101 cattttggac aagtatgtta agcaacacag agatgaaatt gaggcggata agtttgcgac
    11161 tcgtcaatac aaaaaagagg agttcgttga aactcagagt ctggatgatt taattcaaga
    11221 gatgcgtgag gctgtagagg agtcagaagc ttcttcggag gaagttccat cttctgaaga
    11281 catcttacta cccttgcctc tggacgatga ggagcagggc ttggatcctc tattgctaga
    11341 tgatgaaaat ccaacagaaa tgactgaaga agtggaagag gagcaaaacc tttctcgtct
    11401 ggatcaagag gactcagaaa agaaaagtaa aaaaggcttt attttgaccg ctttggcgct
    11461 tgtatcagta attatttgtg tcagtgctta ttatgtctac cgtcaagtgg ctcgttcgac
    11521 taaggaaatt gaaacttctc aatcaactac agccaatcga tcggatgtgg atgattttaa
    11581 tacactttat gacgcctttt acacagatag caataaaacg gctttgaaaa atagccagtt
    11641 tgataaactg agtcaactca aaaccttgct tgataaactg gaaggtagtc gtgaacatac
    11701 gcttgccaaa tctaaatatg atagtctagc aacgcaaatc aaggctattc aagatgtcaa
    11761 tgcacaattt gaaaaaccag ctattgtgga tggtgtgttg gataccaatg ccaaagccaa
    11821 atcggatgct aaatttacgg atattaaaac tggaaatacg gagcttgata aagtgctaga
    11881 taaggctatc agtcttggta agagccagca aacaagtact tctagctcaa gttcaagtca
    11941 aactagcagc tcaagctcta gtcaagcaag ttcaaatacg actagtgagc caaaaccaag
    12001 tagttcaaat gagactagaa gtagtcgcag tgaagtcaat atgggtctct cgagtgcagg
    12061 ggttgctgtt caaagaagtg ccagtcgtgt tgcctataat cagtctgcta ttgatgatag
    12121 taataactct gcctgggatt ttgcggatgg tgtcttggaa caaattctag cgacttcacg
    12181 ttcacgtggt tatatcactg gagaccaata tatccttgaa cgtgtcaata ttgttaacgg
    12241 caatggttat tacaacctct acaagtcaga tggaacctat ctctttaccc ttaactgtaa
    12301 gacaggctac tttgtcggaa atggcgctgg tcatgcggat gacttggact actaagcagt
    12361 cgttacaaaa ttctttcttt tcaaaagtaa aaatgataaa ataaaacaaa tgaaacaaga
    12421 ggagtgtcaa atgacaaaag ctaactttgg tgtcgtaggt atggccgtaa tgggtcgtaa
    12481 ccttgccctt aatattgaat cacgtggtta cacagttgct atctacaacc gtagtaaaga
    12541 aaaaacggaa gatgtgattg cttgccatcc tgaaaagaat tttgtaccaa gctatgacgt
    12601 tgaaagtttt gtaaactcaa tcgaaaaacc tcgtcgtatc atgctgatgg ttcaagctgg
    12661 acctggtaca gatgctacta tccaagccct tcttccacac cttgacaaag gtgatatctt
    12721 gattgacgga ggaaacactt tctacaaaga taccattcgt cgtaatgaag aattggcaaa
    12781 ctcaggtatc aactttatcg gtactggagt ttctggtggt gaaaaaggtg cccttgaagg
    12841 tccttctatc atgcctggtg gacaaaaaga ggcctacgaa ttggttgcgg atgttcttga
    12901 agaaatctca gctaaagcac cagaagatgg caagccatgt gtgacttaca tcggtcctga
    12961 tggagctggt cactatgtga aaatggttca caatggtatt gagtacggtg atatgcaatt
    13021 gatcgcagaa agctatgact tgatgcaaca cttgctaggc ctttctgcag aggatatggc
    13081 tgaaatcttt actgagtgga acaagggtga attagacagc tacttgatcg aaatcacagc
    13141 tgatatcttg agccgtaaag acgatgaagg ccaagatgga ccaatcgtag actatatcct
    13201 tgatgctgca ggtaacaagg gaactggtaa atggacgagc caatcatctc ttgaccttgg
    13261 tgtaccattg tcactgatta ctgagtcagt gtttgcacgc tacatttcaa cttacaaaga
    13321 agaacgtgta catgctagca aggtgcttcc aaaaccagct gccttcaact ttgaaggaga
    13381 caaggctgaa ttgattgaaa aaatccgtca agccctttac ttctcaaaaa tcatttcata
    13441 cgcacaagga tttgctcaat tgcgtgtagc ctctaaagaa aacaactgga acttgccatt
    13501 tgcagatatc gcatctatct ggcgtgatgg ctgtatcatc cgttctcgtt tcttgcaaaa
    13561 gattacagat gcttacaacc gtgatgcaga ccttgccaac cttcttttgg atgaatactt
    13621 cttggatgtt actgctaagt atcaacaagc agtgcgtgat atcgtagctc ttgcggttca
    13681 agcaggtgtg ccagtgccaa ctttctcagc agctattact tactttgata gctaccgttc
    13741 agctgacctt ccggctaact tgatccaagc acaacgtgac tactttggtg ctcacactta
    13801 ccaacgtaaa gacaaagaag gaaccttcca ctactcttgg tatgacgaaa aataagtagg
    13861 tcagccatgg ggaaacggat tttattactt gagaaagaac gaaatctagc tcatttttta
    13921 agtttggaac tccagaaaga gcagtatcgg gttgatctgg tagaggaggg gcaaaaagcc
    13981 ctctccatgg ctcttcagac agactatgat ttgattttat tgaacgttaa tctgggagat
    14041 atgatggctc aggattttgc agaaaaattg agtcgaacta aacctgcctc agtcatcatg
    14101 attttagatc attgggaaga cttgcaagaa gagctggaag ttgttcagcg ttttgcagtt
    14161 tcatacatct ataagccagt cctcatcgaa aatctggtag cgcgtatttc ggcgatcttc
    14221 cgaggccggg acttcattga tcaacactgc agtctgatga aagttccaag gacctaccgc
    14281 aatcttagga tagatgttga acatcacacg gtttatcgtg gtgaagagat gattgctctg
    14341 acccgccgtg agtatgacct tttggcgaca cttatgggaa gcaagaaagt attgactcgt
    14401 gagcaattgt tggaaagtgt ttggaagtat gaaagtgcga ccgagacaaa tatcgtagat
    14461 gtctatatcc gctatctacg gagcaagctt gatgttaaag gtcaaaaaag ctacattaaa
    14521 accgtgcgtg gtgttggata taccattcaa gaatagataa gcagttgcag ttgtgtaact
    14581 gcttttttga ggagttttta tatattgaca tacaatctag tttttactac aatcgtttta
    14641 ggaggatata tagatgaaac ttttgaaaaa aatgatgcaa gttctactag cagtcttttt
    14701 ctttggtttg ctagctacaa atacggtatt tgcgaatacc acaggtggcc gatttgttga
    14761 taaggataat agaaaatatt atgtaaaaga tgatcataaa gcaatctatt ggcataaaat
    14821 agacggtaaa acttactatt ttggtgatat tggagagatg gttgtcggtt ggcaatactt
    14881 agaaatttct ggaacaggtt atcgtgataa tttattcgat aaccaaccag ttaatgaaat
    14941 tggccttcag gagaagtggt actattttgg acaagatggt gctttgttag aacaaacaga
    15001 taaacaagta ctagaggcaa aaacgtctga aaatacagga aaagtatacg gtgaacaata
    15061 tcctctatct gctgaaaaga gaacttatta ttttgataat aattatgctg taaagacagg
    15121 ctggatttat gaagagggtc attggtatta tttaaataag ctaggaaatt ttggcgatga
    15181 ttcttacaat ccactaccaa ttggtgaagt tgctaagggt tggactcaag attttcatgt
    15241 tactattgac attgatagaa gcaaacctgc tccatggtac tacctagatg cttcaggtaa
    15301 gatgcttaca gattggcaaa aagtaaacgg aaaatggtat tattttggct cctctggttc
    15361 tatggcaaca ggttggaaat atgtacgagg caaatggtat tacttagata ataaaaatgg
    15421 tgatatgaag actggctggt atcaagatgg ttcaacatgg tactacctaa atacaagtaa
    15481 tggagatatg aagacaggtt ggttccaggt caatggcaac tggtactatg cttatagctc
    15541 aggtgctttg gcagtgaata cgaccgtaga tggctattct gtcaactata atggcgaatg
    15601 ggttcggtaa tgacagggcg cttataatta tattagaaca ctcgcttaat caaagaagtg
    15661 actttgttgt acttgacctg acgattgggg agattaaaag catgtgaaat ctacacatgc
    15721 tttttcgtta gttatgagtg ttgaacttga ctaaccattg acaaaagata agagcaatta
    15781 aaataagata caaaaatatc aatgctaact gcaccgaaac gcccgatgct atggataggg
    15841 ctaataggga gagcagggac ggggcgagaa tgtccgctgt gcttagagca gagcccaccg
    15901 tccccatctt gtcactgggg atataggcaa agacagctgc ttgtaggcga gggctgagaa
    15961 tgccgataat cgtagaactg atgaaactgg tcatgaaaat tacgatatag gcttgacaaa
    16021 ggatactagt tattagagcc aatagggaaa tctcacagaa aaccaagagg tgcgtgaaac
    16081 ggatattttt aaatagatta ctgctggtca tattgccaag gatagctcca caagacatga
    16141 tgatggagag cagggcgagg agttgcccaa tgctcagttg tgctagtgtc tgttggatga
    16201 agcggagggc aatcagccga ggggacacat ccaagatggc aatcatactg gtcgtcagaa
    16261 acagtagtat gacggtatct tttaacttca gtagcacagc cagcgactgt ttaagatgtt
    16321 gaaaatattt tttaaaactc agtgctgtat gtgacatttc aatccttttt tcaacctcat
    16381 acaaggtatg tcgaataaca tacaggccca aaaaggcaat gacaaaagtc agagtgttga
    16441 taagggaaat agtttgaata cttataacat tgataaggaa tgcgccagcc agattggcga
    16501 caatgcggac aaccctcatc agagactgcc tgaaccccat ggcatcatgc aggtcgtcct
    16561 taattacact gatgtagagg gcgttcatca tgtaggcaga caggtagctc aaggtgtcag
    16621 agatgaggtt gatgatgaca atcataatga ctgaaactaa tgaaaagggc gacaagacga
    16681 gaaatactgt caaaatagcg tagagcagga ttttgatttt ggcaatccag agtgcgtgtt
    16741 tgactctatt ttcttgaaaa tctcctagaa tcccgagaaa gagttgcgat agtaggggaa
    16801 ccatctctga aatggaaatc agtaaaatcg ctagcggagc gaaattgtac tgagccacat
    16861 aattgataat ggcgaggtag aaaatactgt cgccgaatga tgaaaaacag ctgtttagca
    16921 gcaactgtct atatatgttg tttgtccaaa ataatttcat acatttctcc ttatgtctgc
    16981 acatcgaatg ggaatttatt atttaataga caccaatata cccccatgct tcctattccg
    17041 aagtcaaaca aatctgcttt accactatct ccttgaaaca tcagtaaacc gtgatcatct
    17101 cgttcagaac atgctaaaat tacctgttgg atttttttca tcagtaattt gttttgattg
    17161 tagacagtgg tttgtagtaa gctagttatt ccgtggcaat atcctgtcat catttttaga
    17221 tgatggttaa aaacaaattc ttgatattta ctaataatat ctttgtttcc gtcacaat
//
LOCUS       DAVMEF010000029        16897 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMEF010000029 DAVMEF010000000
VERSION     DAVMEF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.029"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(170..1804)
                     /locus_tag="VYQ30_000680"
     CDS             complement(170..1804)
                     /locus_tag="VYQ30_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965836.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLIFFSTIAIHFPFEEMPVYFSFMVAILLVIAFSTSLTAFYNVFYESK
                     DLASYSPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1785..2534)
                     /locus_tag="VYQ30_000681"
     CDS             complement(1785..2534)
                     /locus_tag="VYQ30_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965837.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSSSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2714..4168
                     /locus_tag="VYQ30_000682"
     CDS             2714..4168
                     /locus_tag="VYQ30_000682"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1965838.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAEDAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHMEAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4258..6876)
                     /gene="alaS"
                     /locus_tag="VYQ30_000683"
     CDS             complement(4258..6876)
                     /gene="alaS"
                     /locus_tag="VYQ30_000683"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1965839.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGVKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(6898..7383)
                     /locus_tag="VYQ30_000684"
     CDS             complement(6898..7383)
                     /locus_tag="VYQ30_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1965840.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7658..7897)
                     /locus_tag="VYQ30_000685"
     CDS             complement(7658..7897)
                     /locus_tag="VYQ30_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965841.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8166..9236)
                     /locus_tag="VYQ30_000686"
     CDS             complement(8166..9236)
                     /locus_tag="VYQ30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1965842.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9233..10006)
                     /locus_tag="VYQ30_000687"
     CDS             complement(9233..10006)
                     /locus_tag="VYQ30_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965843.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10003..10809)
                     /locus_tag="VYQ30_000688"
     CDS             complement(10003..10809)
                     /locus_tag="VYQ30_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965844.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10790..11947)
                     /locus_tag="VYQ30_000689"
     CDS             complement(10790..11947)
                     /locus_tag="VYQ30_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965845.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12107..13012)
                     /gene="murB"
                     /locus_tag="VYQ30_000690"
     CDS             complement(12107..13012)
                     /gene="murB"
                     /locus_tag="VYQ30_000690"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1965846.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13124..13876)
                     /locus_tag="VYQ30_000691"
     CDS             complement(13124..13876)
                     /locus_tag="VYQ30_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1965847.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13886..14617)
                     /gene="budA"
                     /locus_tag="VYQ30_000692"
     CDS             complement(13886..14617)
                     /gene="budA"
                     /locus_tag="VYQ30_000692"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1965848.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14759..16030
                     /locus_tag="VYQ30_000693"
     CDS             14759..16030
                     /locus_tag="VYQ30_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1965849.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16050..16865)
                     /locus_tag="VYQ30_000694"
     CDS             complement(16050..16865)
                     /locus_tag="VYQ30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1965850.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
BASE COUNT         5098 a         3808 c         3313 g         4678 t
ORIGIN      
        1 ccaaataaat aaactataaa caacaaaaat agaactatgt tatatttctt attcaaaaca
       61 ttcctcccta tatatttttg attaccaatc ttaatcattt acaactacat tctaacaaac
      121 tataaaagcg tttgtcgaat tgaatttatc aagcaagcga ccaaccagct catctttttt
      181 ctatttctgc caatatgcgt gacaggtaat aatgatagcc aaaaatagca agagcaagca
      241 ggaggataag agctcctact cccaagctga tggcaaggat agtggagaga gactgaacca
      301 agaatatgct cccaattaca agggccatca ggattgcact ataaataaac aataaaacta
      361 tggcgactat gccatttgaa cgattcacca ggtccgtaat gctactccaa ttggttgaca
      421 gatttttaac gtccttaaag taatggtggc aagaaaggat gacactggca aggatccatg
      481 ccacaataag gtaaatcatc gaaatgatag gcaagcctag atatagagaa agaccaagta
      541 aagtcagaat tggtaaaaag gactgaacag caaagataat ccaaaatttc actttcacat
      601 aacgagcaaa gtcaaagggt aaactcttca gaaaatcaac attttccctc tccaaggaca
      661 aggcaattga aggcaggctg gtgatattgt tattgacaac tgctataaac actcctacaa
      721 aaaacaaggg taaccagtat ggaggatgaa tgtctggaac tatctgagaa tctcggattt
      781 tggaaatcag actgatcatc atgagataag gaaggaaagc acttgtaaaa agcactgtaa
      841 ccacgccagt accctgtccc aagagggtga ggtggtagcg taaaaccata cggaaaaagc
      901 cctttttagt agttgaaatc ctctccttgc tgcgacgttc ttttttgacc ttctcctcac
      961 tgttaagcag gatcacgtca taaaaacgag gaaggacctt ctttttggtc agataaagca
     1021 ggaagagagt tagtcctatc caagcgagca gacccaatat ggcttctatt gaaaaaggct
     1081 ccactgctat tttgtaaaag atatgaagag gataaaggag aaatggaatg tctctaactt
     1141 tgtcaacaat acttccaaaa gtcgactgaa gaaagaagat aaatattaaa ggtatgagaa
     1201 ctcctatccc aatcatcaca ttcgaaaaaa tagactgata ctttctgaag accctagtct
     1261 gagccaagaa atgtactgcc actaccatca ctaaagtaac agagacaaat aataaggtca
     1321 aggacagtag catcaaaggc aaacccagcc aaagagaagg agctagacta atatagaggg
     1381 ctagaaaata agctaggatt ggtacaattc cagttagagc tggcaagagg acagacagtc
     1441 ctttagcaat tataatctct gattctttaa aagcataggg actataggag gccaaatcct
     1501 tactctcata aaagacattg taaaatgcag ttaaagaagt tgaaaaggca atcactagta
     1561 aaatagcaac catgaaacta aaataaacag gcatttcctc aaaaggaaaa tgaatggcta
     1621 tagtactaaa aaagatgagc atcaagagac tggaaaaaat gtaagaactt aggactctag
     1681 cggaaacatt tacttttttt ccaggatttt tagcttgctt ctttcgtagg ttagccagat
     1741 tagcttcttg agatgaataa aggatattga tatcaactaa ttttttaatg accttgagac
     1801 gcatccgcaa cctcctcttt tctaccagca aggctaaggt agatactttc caaagactgg
     1861 tctgggttgt cttttctcaa gtcctctacc ttaccacaat aaatcaaatg cccctttttc
     1921 aaaatagcaa tccgatcaca gacttgctca gctacctcta gcacatgagt tgaaaacaaa
     1981 actgtcttac ctttttgcgc atgctccttc atcatttgct tcaaatcaaa ggcagcctgg
     2041 ggatccaaac cagtcaaggg ttcgtccaag acccaaatat caggatcaga caagagtgcc
     2101 ccgatgacaa aaactttctg acgcattcca tgagaaaggg tttcaataac ctgataacga
     2161 ttttcagcaa aatcaaaaac gttcaataac ctagctaaac tagcttccaa gtcagagctg
     2221 ctcaaatcat aggatgaagc aatcaattcc caaaattcat tggccgttaa acgtaaaaat
     2281 aagtcaggcg agtctgctac gtagccaatc tttcgtttga tcgccaagcg attttccgat
     2341 aactcctgac catctaccaa aatacgacca ctgctgggtg aaatgatact gactagggat
     2401 tttatggtgg tcgattttcc agccccatta tgaccaatca agcccataat ctctccattt
     2461 tcaatctgca aattgagatt actcaaggcc tctttatcac catacaactt actaacattt
     2521 tgaaattcaa tcatgggatg actcctatct ttatctatat taaatactat tatactagca
     2581 ctttgataca aaaaaatcaa cactttattt taagtagata caatagtttc cttctattct
     2641 tacgcaaacg tttgcgctaa ccaatacttt atgatagaat aaagaacaag attgacaagt
     2701 aagaggaaaa atcatgcaaa atcaaacgct catgcaatac tttgaatggt atctgcccca
     2761 cgacggtcag cactggacgc gtctggctga agatgctcca cacctagctc atctggggat
     2821 tagccacgtc tggatgccac cagcttttaa ggcaaccaat gaaaaagatg tcggctatgg
     2881 tgtctatgac ttatttgacc taggagagtt taaccaaaaa gggactgtcc gcaccaagta
     2941 tggtttcaaa gaagactatc ttcaagccat tcaagcccta aaagcacagg gaattcaacc
     3001 tatggccgat gtggttctca atcacaaggc tgcagccgat cacatggaag cctttcaggt
     3061 tatcgaagtg gatcctgtag accgtacggt tgaacttgga gaacccttca ccatcaatgg
     3121 ctggactagc tttaccttcg atggtcgcca agatacctac aatgacttcc actggcattg
     3181 gtaccacttc accggtacag actacgatgc aaaacgccgt aagtctggga tttatctcat
     3241 ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta
     3301 cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta
     3361 tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt
     3421 taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg aaaaatacgg
     3481 tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca atctggacta
     3541 tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccatc agaatctctt
     3601 tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag atagcctggt
     3661 tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc aacgaggaca
     3721 agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt
     3781 acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt caggccagta
     3841 tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa aagatttggc
     3901 ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg
     3961 tgctgaaaat caatccccaa tcgcagtctt gatttcaaat gaccaagaaa acagcaagtc
     4021 aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca
     4081 aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt
     4141 aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg
     4201 atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt tacttgatta
     4261 caatttacct gctactgcat ccaacaattc ttggatctta ggttggttgc ttcctcctgc
     4321 catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac
     4381 aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc
     4441 gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa
     4501 ggtacgaagg gcacctgcat cggatacaga cacttgacta gcaatgtaac gatgaccgtt
     4561 gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa
     4621 ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg
     4681 tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag catcctcttg
     4741 ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc gagttcctga
     4801 accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacatg
     4861 agtaccacca caaagttcaa tagagtagtc accaatagtc acgacacgaa cttccttgcc
     4921 gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa tatccgtttc
     4981 aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt caatcgcacg
     5041 caattcttca gcagttacag cttggaagtg ggtaaagtca aagcgaagga attcaacttc
     5101 gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag
     5161 caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa
     5221 ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc
     5281 atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat
     5341 ttgtccgtgg tcagcgacct gtccacccat ttcagcatag aatggcgttt ccgcaaagat
     5401 aagtgaggta gttccttctg atacagcttc tacttctgca ttgtcagcaa cgatagccac
     5461 caatttagaa gacaattggc tagcattgtg gttgaagaca ctttctacag tgatgttttg
     5521 aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac gcgcgcgttc
     5581 ttgttgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac cagcctcttc
     5641 agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt taaagacatc
     5701 ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc cttgggcaaa
     5761 gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa cgattttctc
     5821 aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt ttccaacagt
     5881 tggaacgagt ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc
     5941 acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa gggcaccatc
     6001 accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc tcatgttgtc
     6061 gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa tcggcatgaa
     6121 gaggtccgtt tcaaagttgg tcttaacccc ttggataacg gccaccaaac gctccaaacc
     6181 agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag gaacagcagg
     6241 gtctgcgtta aattgtgaca aaacgatgtt ccagatttcg atgtaacggt cgttttcaat
     6301 atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc cacggtcaaa
     6361 gaaaatctct gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt tgtcctcaat
     6421 tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc
     6481 atctggatag taagtcatgt aaagtttctc agctgggaag tcaaaccatt cagggcttgt
     6541 caaaagctca taagcccaag tgatggcttc atcacggaag taatccccga tagagaagtt
     6601 ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt cgatgtcgtt
     6661 ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa tggtcccgtc
     6721 aaagtatttc ttgagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac
     6781 aggaaccaaa ctgactgatg gttctactga gtgacctttg gtcgcccaga aatcaagcca
     6841 catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt tacttgttta
     6901 atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag
     6961 gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc
     7021 cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat tcaaatccca
     7081 gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc gccaaaaggt
     7141 caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag
     7201 caaggtcaag atttctttac tgatctgact gacttgttca ccagccgcat catagatggt
     7261 aaaagttttg ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc
     7321 atccttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt
     7381 catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt
     7441 accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagta
     7501 gcatgacttt ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg
     7561 aaaattaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca actggaaatg
     7621 atctccgttg aattagacca attccctaca tctctgatta ctttttcagg atatattttt
     7681 tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa
     7741 tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc
     7801 gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc ataaaggttg
     7861 gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag
     7921 gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag
     7981 tacggcaagg caacgctgac atggtttgaa gagtataggc ttagtatact actaggcaag
     8041 caaataagca aataagcaaa taagcaaata aacaactaga atagaaaaag atagggctct
     8101 aaaaactgac ttctattcct taaaaacgaa ccagtttgac tgattcgtct tcttacgttt
     8161 atctcctact tccgatacat tttaaactgt aggaagaggt cgctatattt ccctgtccat
     8221 ttatggtcaa atttctcata aacttctagg tgtttcatgg tttcaacatc gggatagaag
     8281 gccttatctt cctttgtttc ctctgggagc aattccttcg ctggtaggtt tggtgttgaa
     8341 tagccgacat actccgcatt ttggagagca ttttcaggtt tcaacataaa gttgataaag
     8401 gcataggctg agttttggtt tttaactgtt ttgggaatga ccatattgtc aaaccaaaga
     8461 ttgctggcct ctgtcggtac cacataacgt agattttcat ttttttctaa catttggctg
     8521 gcttcaccag agaaggtcac gccgattgca acattattct gaatcatata gcccttcatc
     8581 tcgtccgcaa cgatagcctt gatatttgga gtcagtttgt agagcttatc cactgtctct
     8641 tccaactgct gcagatcctt ggagttgagg ctgtagccga gggaattgag tcctagtccc
     8701 agcacctcac gcgccccatc aaagagcatg atagaattct tatactctgg cttccaaagg
     8761 tcatcccaat gctcaggcgc ttcatctacc atggtttcgt tgtagacaat tcctaaggtt
     8821 ccccagaagt aagggatgga gaatttatta cctgggtcaa aggactggtt gagaaactct
     8881 ggtccgatat tttcgattcc ttcaattttt gaataatcaa gcggaaccaa gaggtcttcg
     8941 tccttcatct tgttaatcat gtattcactt ggaatggcaa tatcgtaggt cgttccaccc
     9001 tgctttatct tagtgtacat ggcttcgttg gagtcaaaag tctcgtactg aacttgaatt
     9061 cctgtttctt ctgtaaactg agtcaagagt tcaggatcga tatagtctcc ccagttatag
     9121 ataaccaatt tttgactatc tcgactattg attttactat ctaaatgagt cgcaattccc
     9181 cacaagacaa ggataatcgc tgcaattcct gctaaaaatg aatagatttt tttcatgctt
     9241 gctcctcctt ctcacgagag ataaagtaat aacctacaac taggataata ctaaagagaa
     9301 agactagagc agacagggca ttgatttcta aggaaatccc tttccgtgca cgagagtaaa
     9361 tctcgactga cagggttgaa aagccatttc ctgttacaaa gaaggtcacg gcaaaatcat
     9421 ccagcgaata ggtgaaggcc atgaagtaac ctgcaatgat agacggagtc aggtaaggaa
     9481 gcatgatttc cttaaacatc tgaaattgac tagctcccaa gtcataggcc gcatgaatca
     9541 tgtcgccatt catttccttg agtcgaggca agaccatcaa gaccacgata ggaatggaga
     9601 aggccacgtg actagataga acggtcaaaa agccaagtga aaacttgagt tgggtaaaga
     9661 gaatcaagaa gctagcacca atcataacgt caggcgcaac catgaggata ttattgagtg
     9721 atagaaaggc ttcttggtat ttcttacgag actggtagat gtaaatggca ccaaaagtcc
     9781 cgataatggt cgctatcaag gctgatagga aggccaagaa aaatgtctga gccaaaatca
     9841 gcatgagtct cccatctcca aacatggttt caaagtgagt ccagctaaaa cctgtaaagc
     9901 tattcatatc atcaccagca ttaaaggcat agccaatcaa gtaaaagata ggcaggtaga
     9961 ggaccagaaa gaccagtccc agataaaggt tggcaaattt tttcatcgtt ctctcctttc
    10021 cttagtcacc cacatggtga tgaacatggt caggatgaga atcacaccga tggttgaacc
    10081 cataccatag ttgtcattgg ttagaaaatt ctgctcaata gccgtcccca aggtgataac
    10141 gcggttccca ccaatcaaac gggtcagcat gaagagactc aaacttggga taaagaccga
    10201 ctgaacccca cttcgcacac cgttcataga tagagggaag atgacatgac ggaaggtctc
    10261 ccacttagtt gcaccaaggt cataactagc attgatgaga ttattatcca tatcgtctaa
    10321 gacattgaaa atcggcaaaa tcataaaggg gagctcgatg tagcttgcga caaagataaa
    10381 ggaaaaatcg gtaaaaagca actgttgtga accaattccg ataaattcca agaattggtt
    10441 aatagagcca ttttgaccaa aaatcccgat aaaagcatag gctttaagga gcaaattgat
    10501 ccaggtagga aggataatca gcatgagcca gagttgacgg tgcttgagac gggtcaaaaa
    10561 gagggccgtc ggataactga taagcagtgt cacaaaggtc acaatgcctg cataaagcac
    10621 tgagttgaaa ctcattttaa gataggtcaa gttttgtgac gcaaagtaag atttgtaatt
    10681 ttctaaactg aactggcctt cgatgttgaa aaaggattga ccgaaaatca agaccaaggg
    10741 tgccaataca aagagcgcaa tccaaagcat gtagggtact acaaagagtt tagagcttgt
    10801 tttcttcatc tctttcctcc tcgattgcat tgatcaaacc tgcttcttgc tcttcgattt
    10861 ctacgtactc ctcaatacga gcatcgaact cttcttcggt ttcattgaga cgcatgatgt
    10921 ggatgtcttc tggttcaaag tccagaccga tttcctcacc cacgatagcc ttacgggttg
    10981 agtggatcat ccattcattt ccaagttcgt cataggcgat aatttcataa tgaactccac
    11041 ggaaaagctg ggtatcgacc ttaacttgga gcttgccttc ttcaggaagg gtaatgcgca
    11101 agtcctctgg acgaataacg acctcaacag gttcatttgg cttcatccca ccatcaaccg
    11161 cttcaaagcg tttgccgtta aattcgacca agtagtcctc aatcatggta cctggcaaga
    11221 tgtttgactc cccgataaag gtggcaacaa agtggttgat tggctcatcg tagatgtcca
    11281 caggggttcc agactggaca atctcgccat cattcataac gaaaatccag tcactcatgg
    11341 caagagcttc ttcctgatcg tgagtgacaa agacaaaggt aatgcccaat cgttgttgta
    11401 attcacgcaa ttcgtactgc atgtctgttc tcaatttcaa gtccagcgct gataaaggct
    11461 cgtccaacaa gaccacacgg ggttggttga tgatagcacg ggcgatggcc acacgctgac
    11521 gttgtcctcc agaaagtttg cggatggaac gtttttcata accttccaac tgaaccatct
    11581 tgagaacttc cgctacacgc tgctcgattt ctttcttatc aattttacgc aagcgaagtg
    11641 gaaaggcaac attttcaaac acattcatat gtgggaacaa ggcataggat tggaagacgg
    11701 tatgtacgtc gcgcttgttg gttggaatat cattgatacg aacaccgtct agcatgatat
    11761 ctcctgtcgt cgcatccagt aaacctgcaa taatgtttag gatagttgat ttccccgaac
    11821 cagatgcacc tagaagggtg tagaatttcc cttcttccaa ctcaaagttg atgtctttga
    11881 gaaccttggt gttgctgtct tcaaaaactt tagagacgtt tttgaattcg ataattggtt
    11941 ttttcaattg gcataaattc cttctttttc atagattaac cgatcggggc tctgtcaggt
    12001 ccccactacc tcttgcaggg agtaaaacca cctgcataca tcttcgctac cgataggctt
    12061 tcaccccctt tccatgacat agcagcagct tttcaaatac agcttcctac ttgctttcac
    12121 ccaagatccg gacttctctt tcaagcgtaa tacctgagtg ttccttgact ttttcgataa
    12181 ccgattggat caagtcctcg tagtctttgg ccgttccatc tgcgacattg atcataaatc
    12241 ctgcatgctt ttctgacact tctacgccac cgatacgata gcctttcaag ccagcttctg
    12301 aaattaactg acctgcaaaa tgcccgactg gacgcttaaa gaccgagcca caagatgggt
    12361 attccaaagg ttgcttgagt tcacgtaggt gcgtcaagcg gtccatttcc tgcttgataa
    12421 cctgatgggt tcctggggct agggcaaatt taactgacaa gacaactgca ccagactcct
    12481 gaatagctga atggcggtaa ccaaaagcca agtctttagc agacagggtt tcgatttctc
    12541 catccttggt caagacctta caagactgca atatgtgagc aatctcgcca ccataggcac
    12601 ccgcattcat aaagacagca ccgccaacgc ttcctggaat accacaagca aactcaaagc
    12661 cagtcaaact atgacggagg gcaatgcgag ttgtttcaat caagttagcc ccagcttctg
    12721 cttcaatggt atagccatca acagaaacgt tattgagctt gtcacacaag atgacaaatc
    12781 cacgaatccc accatcacga acgatgatat tgcttgcatt gccaagaacc atccaaggga
    12841 tattttcttg gttggcaaat ttcacaaggc gcgccaactc aaaacgattt cgtggaaaga
    12901 ccaaataatc agcctctcca cctacttttg tataactata gctatgcaag ggttccttaa
    12961 aacggatatc aattccttct aagatttcaa gcattttttc tcttacagac atgtcactct
    13021 tccttttaca aaattcattc cattatacca tttttagaga catttgacga ccataaaaat
    13081 accttgtttg gattttgcat aagaaaaaga ggttcccccc tttttatgat tttttacaaa
    13141 agatttcctt ggttccatag gcaaccagaa cgagctccag tgctagaatc acttcaacca
    13201 agactggatt tgtcaaccag cctacttgga aaagagatgg tgccagatca aagaaggcat
    13261 gcaagccata ggctgctagg agataaatcc atttcttctg gcgaacagct tggtaaaccc
    13321 aaactgtcaa aagtaattgg aaaccaagcg ccaagattcg ctcaaaacca agcaaataaa
    13381 tctgccagac cgaaagtgac tgaatggttt ttaacatatt ttcagacagt aattgcataa
    13441 cctgtggatt ctgagtttga actgccgaaa gaacgatgta aagattgagt aaactagtaa
    13501 ggcctagaaa aatcaactcc aagccaccat gccctaggcc ataggccaag gcatctgcct
    13561 tttccaaact tctctttttc tccaaccatt tgaagaaaac aagacgagcc gtttcctcaa
    13621 aaaatgctgc catggctagg ccatagataa tatagataag cggatgatct tgcaagaggg
    13681 caatactacc gtctttttga ggatgcaaaa tcaagatatg caccagtttt tccaaaatct
    13741 gcgaagagac aaagaaagcg actgcaccca agcccaagac agctagatta atctggtatt
    13801 tctttttggc aaaccaaatg cttcctatca gaaaagctag caacagcaac atggtaataa
    13861 tgatatgaat ggtcattttc ttctcctatt ctgccttttc aatatctttt ttcatctcgt
    13921 caacattgaa cttagcaaac aagtattgac ggtcttggac tgggaaacgt tggtccaact
    13981 ggtcaactgc tcccacctcg ataatgcctt ccttgatgac aaaatccatg acatgtccac
    14041 cgaaggtcaa atcatctgat atgaagtgca gatggtagcc tgccacactg accccatgga
    14101 aaatctcagg cgtccagaaa ccaacaatag tccccgcaac attgtcacga ctatattccg
    14161 gttgatgggt tgcgacatca gcaaacttgg tatcgggtgt tgacttagga atcatacgca
    14221 catgcatatg tgaaaattcc ccccgaatct tgatagagcg gaaaagattt tccccatcat
    14281 aataagactc aattcgttct ttcaattcct tgtctgtcat ctcaaagcgc tggcggaaaa
    14341 tgacctctgc ctgatgcggt accactgcag cgtaaggaat aagggcatct ggtgacactt
    14401 ccacaatttc tggctggtct cctgaccctt tggcctgata agccttgcca tccaagacaa
    14461 tcaattcccc atcaatggaa tccaaggttc ccaaaccaag gtcaccatgt tctagcaatt
    14521 ctcctactgt catagtacca ccataaaggc cagccatcaa ggctccaaga gtattgtatt
    14581 gaaataattt cactggttcc tgcacgttct tatccattca ctttcttgtc tgttattcta
    14641 taaatcttac tcctaagtat accacatttg cccctagatg tgaacgagag aaacgctcta
    14701 gacattgcca agaaggaaaa aaaagggtac aatgtaacaa aatcaaggga ggtctggaat
    14761 gaagaaacaa agcaagtaca aagaggtcgt ttcctatctg aaaaatggta tcgagtctgg
    14821 acgatttccg acgggtagtc gcctgccttc tatccgtcaa ctgagccttg actttcactg
    14881 cagcaaggac accattcaac gagccctgct ggaattacgg cacgaacaat acctctatgc
    14941 caagcctcag agtggctact atgtattaga acaagggcaa catcaagacc tagaaatcga
    15001 ggttaccgac gaacatgcca gtgcctatga cgatttccga ctctgtgtca atgaaacctt
    15061 gattggacga gaaaactacc tcttcaacta ctatgacaat caagaaggat tagaagacct
    15121 aagacagtcc attcacaaac tcctctttga gcaagctctc tactgcaagg ctaaccaact
    15181 agtactgact tctggaaccc aacaagcctt atttatcctc tctcaaatat cctttcctag
    15241 acaagccaag gaaatcttgg tggaacagcc aacctaccat cggatgaatc gcctcttgat
    15301 tgcacagggg ctggactatc aaacgattga acgaggcatt gatgggattg acttggagga
    15361 gctggaaggc cacttcaaaa caggaaaaat taagtttttc tacaccattc cccgatttca
    15421 ctatcctctg ggacattcct attctgagca agacaaacga tccattctta acttagctgc
    15481 caagtatgat gtctatatcg tagaagacga ttatctgggt gatttggact ccaagaaggg
    15541 ccaaaccttc cactatcttg atacagagga gcatgtcatt tatatcaagt ccttctcgac
    15601 cagccttttt cctgcccttc gtattacagc actcattctt ccaaatgcta tcaaagaagc
    15661 atttgtggcc tacaaaaata tcctagacta cgacagcaac ctcattatgc aaaaggccct
    15721 gtcactctat attgacagtc aattgtttga aaaaaatcgt ttggctcgct tgaccaatca
    15781 tgaatcttac caaaaacaaa tcgaggaaag gataactaaa acaccttgtc cccttcctca
    15841 ttatccccta cacgatggtt tattgctaga cctgagacag tatcctaaaa tcgccagtct
    15901 caaacacagt caaccgggct tggacttctt tgaagaggcc tatttaagta cctgtcctta
    15961 tcaatttgcc aaggtgtccc tagacaatct ggaaaatgtt ttaaactatt taaaagcaga
    16021 attggaatga catccaactc tgctttttct tattcttcaa cttcagttag acttgctgct
    16081 ttttcaagat agctttggta aattccttca tccttaagtt tttggatgac cttgtctacc
    16141 acttctttca aatcatcact attttttcta agagcaacgg cattggcgtc gccgtccttc
    16201 atcttcaagc tgacagttgc gacagctaag ccagcgtttt tagcagcata actaagtgca
    16261 acaggctcat ccatatgaac agcatctact tttccagcct gcaattcatt gactgcttca
    16321 cccatattag ttagggaagt taattgagct tttggcaatt gttccttgac cattgattct
    16381 ggaacagtcc ctttttgggc tgcaatatta gcactttcta ggctagttaa atccttgtat
    16441 ttttccacat cagccttacg aaccaagaaa ctaatcttgt tttcatagta tgggattgaa
    16501 aaatcaaaga cttcttttct ctcgtcagta gcactaattc ctgcaactgc taggtcagcc
    16561 ttaccagttt gaagactggt caaaacattg tcaaaactca tgcttgagat ttccaactta
    16621 accccaagtt catcagcgat agcctgagcc atgtcgatgt ctgcaccgac tacctggttc
    16681 tttccatcaa ccaatgattg aaattcaaag ggtgcatagt caggactggt tgccacaact
    16741 aatttcccct tttgcttaat ggcctcaaca gctgactgag aaccattaga acctgactgg
    16801 caagctacta agaagaaact tgcaagaaaa ttacataaaa caaatatcca tttttttgat
    16861 ttcataaata aacaacctct ctgttaattt tgtataa
//
LOCUS       DAVMEF010000030        16722 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMEF010000030 DAVMEF010000000
VERSION     DAVMEF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.030"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            16..1008
                     /locus_tag="VYQ30_000695"
     CDS             16..1008
                     /locus_tag="VYQ30_000695"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1965851.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEIIGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            1073..1612
                     /gene="rsmD"
                     /locus_tag="VYQ30_000696"
     CDS             1073..1612
                     /gene="rsmD"
                     /locus_tag="VYQ30_000696"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1965852.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDTER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            1602..2090
                     /gene="coaD"
                     /locus_tag="VYQ30_000697"
     CDS             1602..2090
                     /gene="coaD"
                     /locus_tag="VYQ30_000697"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1965853.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            2074..3111
                     /locus_tag="VYQ30_000698"
     CDS             2074..3111
                     /locus_tag="VYQ30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1965854.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKEIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            3169..3399
                     /locus_tag="VYQ30_000699"
     CDS             3169..3399
                     /locus_tag="VYQ30_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1965855.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            3423..4706
                     /gene="murA"
                     /locus_tag="VYQ30_000700"
     CDS             3423..4706
                     /gene="murA"
                     /locus_tag="VYQ30_000700"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1965856.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEANDEDE"
     gene            4699..4887
                     /locus_tag="VYQ30_000701"
     CDS             4699..4887
                     /locus_tag="VYQ30_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1965857.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            4926..5750
                     /gene="endA"
                     /locus_tag="VYQ30_000702"
     CDS             4926..5750
                     /gene="endA"
                     /locus_tag="VYQ30_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1965858.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            6041..7372
                     /locus_tag="VYQ30_000703"
     CDS             6041..7372
                     /locus_tag="VYQ30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1965859.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(7571..7641)
                     /locus_tag="VYQ30_000704"
     tRNA            complement(7571..7641)
                     /locus_tag="VYQ30_000704"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7607..7609),aa:Cys,seq:gca)
     gene            8057..11668
                     /gene="rpoB"
                     /locus_tag="VYQ30_000705"
     CDS             8057..11668
                     /gene="rpoB"
                     /locus_tag="VYQ30_000705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1965860.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            11701..15378
                     /gene="rpoC"
                     /locus_tag="VYQ30_000706"
     CDS             11701..15378
                     /gene="rpoC"
                     /locus_tag="VYQ30_000706"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1965861.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            15489..15902
                     /gene="ndk"
                     /locus_tag="VYQ30_000707"
     CDS             15489..15902
                     /gene="ndk"
                     /locus_tag="VYQ30_000707"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1965862.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT         4923 a         3272 c         3866 g         4661 t
ORIGIN      
        1 tttgaggagt tgtctatgaa gaaaagtttt atccatcaac aagaagaaat ttcctttgtc
       61 aaaaacactt ttacccagta tttgaaagat aagctagaag ttgtcgaagt tcaaggtcct
      121 atcttgagta aggtcggtga cggaatgcag gacaacctgt ctggtgtgga aaatccagta
      181 tcggtcaagg ttctccaaat ccctgatgct acttatgaag tggtgcactc acttgctaaa
      241 tggaaacgcc acaccttggc tcgttttggc tttggtgaag gagagggtct ctttgtccac
      301 atgaaagccc ttcgtccaga tgaggattcc ttggatgcaa cccactctgt ttatgttgac
      361 cagtgggact gggagaaggt tattccaaat ggtaagcgta acatcgttta tctaaaagaa
      421 acagttgaga agatttataa ggctattcgc ctgactgagc tagctgttga agcccgctat
      481 gacatcgagt ctatcttgcc aaaacaaatc acctttatcc atacagaaga attggtagaa
      541 cgctacccag acttgacacc taaagaacgt gaaaatgcga tttgtaaaga atttggagcc
      601 gtctttttga ttggtatcgg tggcgagttg ccagatggta aaccgcacga tggacgtgca
      661 ccagactatg atgactggac aagcgagtct gagaatggct acaagggtct aaatggtgat
      721 attcttgtct ggaatgagtc tttaggtgga gcctttgagt tgtcttctat gggaattcgt
      781 gtagatgaag aaactcttag acgtcaggtg gaaattatag gtgatgaaga ccgcttagaa
      841 ttggaatggc acaagtcttt gttgaatggc ctattcccat tgacaatcgg tggaggaatt
      901 ggacaatctc gtatggccat gttcctactt cgcaagagac acatcggaga agtgcaaaca
      961 agtgtttggc ctcaagaagt ccgcgatact tacgaaaata ttttgtagag aatcgaaccg
     1021 caaggttcgg ttttctttct ctttttgtct ataatttggt ataataaaca gtatgaaaat
     1081 cgtatcagga atctatgggg gacgtcccct caagacacta gaaggcaaga caacaagacc
     1141 tacttcggat aaggttaggg gagccatttt taacatgatt ggtccctact ttgaagtggg
     1201 acgagtcttg gacctttatg caggtagtgg tggtttatct atcgaagcag tatcgcgtgg
     1261 catgtccagt gctgttttgg tggagcgaga ccgtaaggct cagaccatcg tggctgaaaa
     1321 tatccagatg accaaggaag ttggaaaatt tcaactcctc aaaatggata cagaaagggc
     1381 attggaacag gtatctgggg aatttgacct cgttttctta gaccctccct atgccaagga
     1441 acaaatcgta gcagatattg aaaaaatggc tgagagagag cttttttctg aagatgttat
     1501 ggttgtgtgc gagacggata agaccgttga acttccagaa gaaattgcct gtctgggtat
     1561 ctggaaggaa aagatttatg gaattagtaa ggtgacagtc tatgtcagat aagattggct
     1621 tattcacagg ctcatttgat ccgatgacaa atgggcatct ggatatcatt gaacgggcga
     1681 gcagactttt tgataagctt tatgtgggta ttttttttaa tccccacaaa caaggatttc
     1741 tccctcttga aaatcgtaaa cgggggctag aaaaggcttt gggacatttg gaaaatgttg
     1801 aagtcgtggc ttctcatgat aaattggtgg tcgatgttgc aaaaagattg ggggctactt
     1861 ttctagtacg ggggttgagg aatgcggcgg atttgcaata tgaagctagt tttgattact
     1921 acaatcatca gctgtcttct gatatagaga ctatttattt acatagccgg cctgaacatc
     1981 tctatatcag ttcatcaggt gttcgagagc ttttgaagtt tggtcaggat attgcctgcc
     2041 atgttcccga gagtattttg gaggaaataa gaaatgaaaa aaaagattag atggcctttg
     2101 tatgtcattg cggccttgat tgtgaccttc ttggcatttg tggtgccttt gccctactat
     2161 atagaggttc caggtggttc ggaagatatt cgccaagtcc ttaaagtaaa tgacacagaa
     2221 gataaggaag ctggtgccta tcaattcgtt acggttggtg ttcaacatgc tactttagct
     2281 catatgattt atgcttggtt gacacctttt acagatattc gtagtgctca ggagactaca
     2341 ggtggttctt ccgatgttga atttatgcga atcaatcaat tctacatgca aacatcgcaa
     2401 aatatggcca agtatcaagg actaaaaaca gctggtaagg aaatcgaact caagtacttt
     2461 ggagtttatg ttttgaatgt gacggataat tcaaccttta aagggattct caatatctct
     2521 gatacagtca cagcagtcaa tgatcagacc tttgatagtt ccaaagactt gattgattac
     2581 gtcagttctc aaaaattagg ggactccgtc aaggtcacct atgaagagga tgggcaaacc
     2641 aagtctgcag aaggaaagat tatcaccttg gaaaatggca aaaatggaat tggaatcggc
     2701 ttgattgacc gtacagaggt aatcagcaat gtcccaatta gcttttcaac agctggtatt
     2761 ggcggtccaa gtgctggtct catgtttagt ctagctatct atactcaaat agctcaccca
     2821 gatcttcgta atggtcgtat tgttgccggt acaggtacca ttgaccgcga tggtaatgtg
     2881 ggagacattg gaggtattga taagaaggtt gtagcttcgg ctagggcagg tgctgctatt
     2941 ttctttgctc ctgataaccc tgttagcgaa gaagaacaaa aggcgcatcc ggacgcgaaa
     3001 aacaactacc aaacagccct agaagcggct aaaacaatca agacggatat gaaaatcgtg
     3061 cccgttaaaa ccctacaaga tgcgattgat tacttgaaaa acaatcccta atttttgagt
     3121 gaagtttcca aactagcgca caaacagaga agatggtata atagtcaaat ggttcaatta
     3181 ttattcactc taagtagtca tatgctcttt atttatgtga gtttttacct tttaaaggat
     3241 cttgttagat gggagaaggt tttaaaagtg acagatgata atacaagaaa agttcgttta
     3301 ttagtagcct tttttagcat tgtcataggc tacatcctga gttctttctt tattagcctg
     3361 tatcatttgt ggcaagaagc gcttagagga ttattataaa atcaagagta aaggaaacga
     3421 gtatggataa aattgtggtt caaggtggcg ataatcgtct ggtaggaagc gtgacgatcg
     3481 agggagcaaa aaatgcagtc ttacccttgt tggcagcgac tattctagca agtgaaggaa
     3541 agaccgtctt gcagaatgtt ccgattttgt cggatgtctt tattatgaat caggtagttg
     3601 gtggtttgaa tgccaaggtt gactttgatg aggaagctca tcttgtcaag gtggatgcta
     3661 ctggcgatat cactgaggaa gccccttaca agtatgtcag caagatgcgc gcctccatcg
     3721 ttgtattagg gccaatcctt gcccgtgtgg gtcatgctaa ggtatccatg ccaggtggtt
     3781 gtacgattgg tagccgtcct attgatcttc atttgaaagg tctggaagct atgggggtta
     3841 agattagtca gacagctggt tacatcgaag ccaaggcaga acgcttgcat ggcgctcata
     3901 tctatatgga ctttccaagt gttggtgcaa cgcagaactt gatgatggca gcgactctgg
     3961 ctgatggggt gacagtgatt gagaatgctg cgcgtgaacc tgagattgtt gacttagcca
     4021 ttctccttaa tgaaatggga gccaaggtca aaggtgctgg tacagagact ataaccatta
     4081 ctggtgttga gaaacttcat ggtacgactc acaatgtagt ccaagaccgt atcgaagcag
     4141 gaacctttat ggtagctgct gccatgactg gtggtgatgt cttgattcga gacgctgtct
     4201 gggagcacaa ccgtcccttg attgccaagt tacttgaaat gggtgttgaa gtaattgaag
     4261 aagacgaagg aattcgtgtt cgttctcaac tagaaaatct aaaagctgtt catgtgaaaa
     4321 ccttgcccca cccaggattt ccaacagata tgcaggctca atttacagcc ttgatgacag
     4381 ttgcaaaagg cgaatcaacc atggtggaga cagtttttga aaatcgtttc caacacctag
     4441 aagagatgcg ccgcatgggc ttgcattctg agattatccg tgatacagct cgtattgttg
     4501 gtggacagcc tttgcaggga gcagaagttc tttcaactga ccttcgtgcc agtgcggcct
     4561 tgattttgac aggtttggta gcacagggag aaactgtggt cggtaaattg gttcacttgg
     4621 atagaggtta ctacggtttc catgagaagt tggcgcagct aggtgctaag attcagcgga
     4681 ttgaggcaaa tgatgaagat gaataagaaa tcaagctacg tagtcaagcg tttactttta
     4741 gtcatcatag tactgatttt aggtactctg gctctaggaa tcggtttaat ggtaggttat
     4801 ggaatcttgg gcaagggtca agatccatgg gctatcctgt ctccagcaaa atggcaggaa
     4861 ttgattcata aatttacagg aaattaggct ggagaaccag cctttttcta aagataagga
     4921 gaaatatgaa caaaaaaaca agacagacac taatcggact gctagtgtta ttgcttttgt
     4981 ctacagggag ctattatatc aagcagatgc agtcgacagc taatagtcct aaaaccaagc
     5041 ttagtcagaa aaaacaagcg cctgaagctc ctagtcaagc attggcagag agtgtcttaa
     5101 cagacgcagt caagagtcaa ataaagggga gtctggagtg gaatggctca ggtgctttta
     5161 tcgtcaatgg taataaaaca aatctagatg ccaaggtttc aagtaagccc tacgctgaca
     5221 ataaaacaaa gacagtgggc aaggaaactg ttccaaccgt agctaatgcc ctcttgtcta
     5281 aggccactcg tcagtacaag aatcgtaaag aaactgggaa tggttcaact tcttggactc
     5341 ctccaggttg gcatcaggtc aagaatctaa agggctctta tacccatgca gtcgatagag
     5401 gtcatttgtt aggctatgcc ttaatcggtg gtttggatgg ttttgatgcc tcaacaagca
     5461 atcctaaaaa cattgctgtt cagacagcct gggcaaatca ggcacaagcc gagtattcga
     5521 ctggtcaaaa ctactatgaa agcaaggtgc gtaaagcctt ggaccaaaac aagcgtgtcc
     5581 gttaccgtgt aaccctttac tacgcttcaa acgaggattt agttccctca gcttcacaga
     5641 ttgaagccaa gtcttcggat ggagaattgg aattcaatgt tctagttccc aatgttcaaa
     5701 agggacttca actggattat cgaactggag aagtaactgt aactcagtaa aagataagcc
     5761 taaactccta tgtcacttat ggatgtagga gttcttttta ctagtttaag cagggctaga
     5821 acaggtacta agaaaaaata gtaacttcta aaactaactt ccagtttccc acaacctagc
     5881 ttttagtatg agaaaaacgc gtgaaatcag tggaaatccg tcttagacta acttccagtt
     5941 ttgggagaga gatggaagtt acgctaagaa aaaaataata tgtattggat taaattttgt
     6001 gatataataa aggataagat actattttag gagaagaact atggaagacc caagtagtca
     6061 gaatttgttg ctacaatttg ttttattgtt tatcttgacg gtattaaatg cctttttctc
     6121 agccacagaa atggccatgg tttctcttaa tcgtgcccga gttgaacaaa aggcggaaga
     6181 aggagacaga cgctacatcc gcctgctgaa ggtactagaa aatcctaacc actttttatc
     6241 aaccattcag gtaggaatca ccctgattac aatcttatca ggggcgagtt taactgatac
     6301 tctaggacgt gtgattgcct cttggcttgg gaatggcgaa acagcacaag ccgtggcaac
     6361 ttttctatcc ttggcatttt tgacctatat ttccatcgtt tttggggaat tatatcctaa
     6421 gagaatcgct cttaatctaa aggatgcctt ggcaattcgt acagcgccga ttatcattgg
     6481 gatcggaaaa ctagtcagtc cttttgtttg gcttttagct gcttcgacca atttcttgag
     6541 ccatttgact cctatgtcat tcgatgatgc ggatgaaaag atgacccgtg atgaaattgc
     6601 ctatatgctg acaaatagtg aagaaacatt ggatgctgat gagattgaga tgctacaagg
     6661 tgtcttttcg ctcgatgaac tgatggcacg agaggttatg gttcctcgaa cggatgcctt
     6721 tatggtggat attcaggatg atagtcaagc cattatccaa agtattttaa aacaaaatta
     6781 ttctcgtatc ccggtttatg atggggataa ggacaatgta attggaatca ttcacaccaa
     6841 gagtctcctt aaggcaggct ttgtggacgg ttttgacaat attgtttgga agagaatttt
     6901 acaagatcca ctttttgtac ctgaaactat ttttgtggat gacttgctaa aagaactgcg
     6961 aaatacccaa agacaaatgg caatcttgct ggatgaatac ggtggtatgg ctggtttggt
     7021 gactttggaa gacctcttag aggaaattgt tggtgaaatt gatgatgaaa cggacaaagc
     7081 agcaatagat gttcatcaga ttggcgagga tacctacatt gttcaaggaa ctatgaccct
     7141 caatgatttc aataattact ttgatgttga actggaaagt gatgacgttg ataccatcgc
     7201 tggttattat ttgacaggag tgggaaccat tccaacgact gagaaactca gctatgaatt
     7261 ggtcagccaa aacaaacagt ttatcttaac caatgataaa gtgaagaatg gacgtgttac
     7321 caaggtaaaa gtccaaatca cagaagtcga aatagaagaa gaaacagaat aaactagagg
     7381 cttttgtcac caggcgtgac gaagcttttt tcaattgatt tcaaaaggga attatgccat
     7441 ggaaagaact ctgccacaaa ctcgctccaa aagtttttaa agttatacgt atttattcga
     7501 gggaaaataa aaaaaagccc ttcaaattgg gctttctgtt catttgaaac ctgataaatc
     7561 aagttataga aggcggtaga cggatttgaa ccgacgatca agcttttgca gagccgtgcc
     7621 ttaccacttg gctataccgc cttaacgttt attattatac cttgaaaatg attttcagtc
     7681 aatagttttt tcagtttttt gtaccatagc gtgattcgaa tgtgatgcca agttttctat
     7741 gggaaaaaat ttaccaaaaa cctactgaaa ccccttgaaa aatctagcta aatagggtat
     7801 aatatgggta atcatttgtc gtaggttttg tctgaaatat tgtccagaca aggctcacag
     7861 cagttaaatc ttctgaaaaa gtcagattta gctgctcttt ttgtgctttt tttcaagatt
     7921 ttgagcattt gtaacagagg cttaaagatt ctgaaaattc gtcaagagga cacggtgata
     7981 aggggtttac aaccatatgg cgattagaaa agcctgattg acaaggcttg gaacttattt
     8041 acaaaggaga atcatcttgg caggacatga cgttcaatac gggaaacatc gtacccgtcg
     8101 tagtttttca agaatcaaag aagttcttga cttaccaaat ttgattgaaa ttcaaactga
     8161 ctcattcaaa gctttcctag accacggtct taaggaagtg tttgaagatg tattgccaat
     8221 ttcaaacttc acagacacaa tggagttgga atttgttgga tatgaaatca aggaaccaaa
     8281 atacacgcta gaagaagctc gtatccacga tgctagctac tcagcaccaa tttttgtaac
     8341 cttccgcttg atcaataaag aaacaggcga aatcaagacc caagaagttt tctttggtga
     8401 tttcccaatc atgacagaaa tgggtacttt catcatcaat ggtggtgaac gtattatcgt
     8461 ttctcagttg gtccgctcac caggtgttta ctttaacgac aaagtagaca aaaatggtaa
     8521 ggtgggctat ggttcaactg ttatccctaa ccgtggagct tggttggaac ttgaaagcga
     8581 ctcaaaagat atcacctaca ctcgtatcga ccgtactcgt aagattccat ttacaacctt
     8641 ggttcgtgct cttggtttct caggtgatga tgaaatcttt gatatctttg gtgacagcga
     8701 attggttcgc aacactgttg aaaaagatat ccacaagaat ccaatggact ctcgtacaga
     8761 cgaagccttg aaagaaattt acgaacgcct tcgtccaggt gagcctaaga cggctgaaag
     8821 ctcacgtagc ttgcttgtag ctcgcttctt tgacccacgt cgttatgact tggcagcagt
     8881 tggtcgttac aaaatcaata aaaaactcaa tgttaaaaca cgtttgctca accaaaccat
     8941 tgcagagcca ttggtagacc ctgaaactgg agaaatcttg gtagaagctg gtacgattat
     9001 gactcgtagc gtgattgaaa gcattgaaag ccatttggat ggcgacttga acaagattgt
     9061 ctacatccca aacgatgcag ccgttgtgac tgagcctgtt gttcttcaaa aattcaaggt
     9121 tattgctcca actgatccag atcgcgtcgt aacgatcatt ggtaatgcta acccagatga
     9181 caaggttcgt acggtgactc ctgcagatat ccttgctgag atgagctact tcctcaactt
     9241 ggctgaagga cttggccgtg tagatgatat cgaccacctt ggaaatcgtc gtatccgtgc
     9301 ggttggtgaa ttgcttgcca accaagtacg tttgggactt tctcgtatgg aacgtaatgt
     9361 ccgtgaacgt atgtctgttc aggacaatga agtcttgaca ccacaacaaa ttatcaatat
     9421 ccgtcctgta acagctgcag ttaaagaatt ctttggttca tcacagttgt cacagttcat
     9481 ggaccaacac aacccgcttt ctgagttgtc tcacaaacgc cgtttgtcag ccttaggacc
     9541 tggtggtttg actcgtgacc gtgctggata tgaagtacgt gacgtgcact acactcacta
     9601 tggtcgtatg tgtccaatcg agacacctga aggacctaac atcggtttga tcaataactt
     9661 gtcatcttat ggacacttga acaaatatgg ttttgttcaa acaccatacc gtaaggttga
     9721 ccgtgaaaca ggtgttgtca cgaacgaaat tgtttggttg acagctgatg aagaagatga
     9781 atatactgta gctcaggcta actctcgtct gaatgaagat ggaacctttg ctgagaagat
     9841 tgtcatggga cgtcaccaag gggtcaacca agagtatcca gctaatattg ttgactacat
     9901 ggacgtttca ccaaaacagg tagttgccgt tgcgacagca tgtattcctt tcttggaaaa
     9961 cgatgactcc aaccgtgccc tcatgggagc caatatgcaa cgtcaggctg tgccattgat
    10021 taatcctcag gcaccttacg ttggtactgg tatggaatac caagcagccc acgattctgg
    10081 tgcggctgtg attgctcagt atgatggtaa agttacttac gcagatgctg acaaggtaga
    10141 agttcgtcgt gaagatggtt cattggatgt ttaccacatc caaaaattcc gtcgttcaaa
    10201 ctcaggtact gcttacaacc aacgcactct cgtaaaagtt ggtgatgtcg ttgaaaaagg
    10261 cgatttcatc gctgacggac cttctatgga aaatggagaa atggcgcttg gacaaaaccc
    10321 aatcgttgcc tacatgactt gggaaggtta caacttcgag gatgccgtta tcatgagcga
    10381 acgcttggtg aaggacgatg tctacacatc tgttcacctt gaagaatacg aatcagaaac
    10441 gcgcgataca aagcttgggc ctgaagaaat cactcgcgaa attccaaacg ttggtgaaga
    10501 tgccctcaaa gaccttgacg aaatggggat tatccgtatt ggtgctgagg ttaaagaagg
    10561 tgatattctt gtaggtaaag taacacctaa gggtgagaaa gatctttcag ctgaagaacg
    10621 tctcttgcac gctatctttg gagacaagtc tcgtgaagtg cgtgatactt ctcttcgtgt
    10681 accacacggt gccgatggtg tcgttcgtga tgttaagatc tttacacgtg taaatggaga
    10741 tgagttgcaa tcaggtgtta acatgttggt tcgtgtttac atcgctcaaa aacgtaagat
    10801 taaggtcgga gataaaatgg ccggacgtca cggaaacaaa ggggttgtct ctcgtatcgt
    10861 tcctgtagaa gacatgcctt accttccaga cggaactcca gtcgacatca tgttgaaccc
    10921 acttggggta ccatcacgta tgaatatcgg tcaggttatg gagcttcacc ttggtatggc
    10981 agctcgtact cttggtattc acatcgctac accagtcttt gatggagcaa gttctgaaga
    11041 tctttggtca actgttaaag aagcaggtat ggatagcgat gccaaaacga tcctttacga
    11101 tggacgtact ggtgaaccgt ttgataaccg tgtttctgtc ggagtcatgt acatgatcaa
    11161 actccaccac atggttgatg ataaattgca cgcgcgttca gtcggacctt actcaactgt
    11221 tacccaacaa ccactcggag gtaaagctca gtttggtgga caacgtttcg gtgagatgga
    11281 ggtttgggct cttgaagcct acggtgcgtc aaatgtcctt caagaaatct tgacttacaa
    11341 gtcggacgat atcaacggac gtttgaaagc ctatgaagct attacaaaag gcaaaccaat
    11401 tccaaaacca ggtgttccag aatccttccg agttcttgtc aaagaattgc aatctcttgg
    11461 tcttgacatg cgtgtcctag acgaagatga ccaagaagtg gaacttcgcg acttggatga
    11521 aggaatggac gaagatgtca tccacgtaga tgaccttgaa aaagcccgcg aaaaagcagc
    11581 ccaagaggct aaagcagcct ttgaagctga agaagctgag aaagcaacaa aagcggaagc
    11641 aacagaagaa gctgctgaac aagaataagc agttcactta gaatagaaag ggaagaaata
    11701 gtggttgatg taaatcgttt taaaagtatg caaatcactc tagcttctcc aagtaaagtc
    11761 cgttcatggt cttatggaga agtcaaaaaa cctgaaacaa tcaattatcg taccttgaaa
    11821 ccagaacgtg aaggactctt tgatgaagtg atctttggtc ctacaaaaga ctgggaatgt
    11881 gcttgtggta agtacaaacg cattcgttac agaggaattg tttgtgaccg ctgtggggtt
    11941 gaagtaacgc gtacgaaagt tcgtcgtgag cgtatgggac atatcgaatt gaaagctcct
    12001 gtatctcaca tctggtactt caaggggatt ccaagccgta tgggcttgac ccttgatatg
    12061 agccctcgtg ccctcgagga agttatctac tttgcggctt atgtggtgat tgatcctaag
    12121 gatacaccac ttgagcacaa gtctatcatg acagagcgcg aataccgaga gcgcttgcgt
    12181 gaatatggtt atggttcatt tgttgccaag atgggtgcgg aagccatcca agaccttttg
    12241 aagcaagtag atcttgaaaa agaaattgct gaactcaaag aagaattgaa aacagctact
    12301 ggacaaaaac gtgtcaaagc catccgtcgt ttggatgttt tggatgcctt ttacaagtct
    12361 ggaaacaaac ctgaatggat gattcttaac atccttccgg ttatcccacc agatcttcgt
    12421 ccaatgttgc agttggatgg tggccgtttt gcctcatctg acttgaatga cctttaccgc
    12481 cgtgttatca accgtaacaa ccgtttggct cgtttgcttg agttaaatgc accaggtatc
    12541 atcgttcaaa atgagaagcg tatgcttcaa gaagcagttg atgctttgat tgacaatggt
    12601 cgtcgtggtc gtccaatcac aggaccaggt agccgtccat tgaaatcatt gagccacatg
    12661 cttaaaggta aacaaggacg cttccgtcaa aacttgctcg gtaaacgtgt tgacttctca
    12721 ggacgttccg ttatcgccgt tggtccaact cttaagatgt accaatgtgg tgtgccacgt
    12781 gaaatggcga ttgaactctt taaaccattt gtcatgcgtg aaatcgttgc ccgtgatatc
    12841 gtgcaaaacg tcaaagcagc taaacgcttg gtggaacgcg gagatgagcg tatctgggat
    12901 atccttgaag aagtgattaa agaacaccca gtgcttttga accgcgcacc gacccttcac
    12961 cgtttgggta tccaagcctt cgagccagtc ttaattgatg gtaaggctct tcgcttgcac
    13021 ccacttgtct gtgaagccta caatgctgac tttgacgggg accaaatggc catccacgta
    13081 ccactttcag aagaagcaca agcagaagct cgtatcctca tgctagctgc tgagcacatc
    13141 ttgaacccga aagatgggaa accggtagtt actccatctc aggacatggt tttgggtaac
    13201 tactacttga ccatggaaga agctggtcgc gaaggtgaag gaatggtctt caaagaccgt
    13261 gacgaagcgg ttatggctta ccgcaatggt tatgttcacc tccactcacg tgttggtatc
    13321 gcaacagaca gcctcaacaa gccttggaca gaagagcaaa gacataaggt cttgcttaca
    13381 acagttggta aaattctctt caacgatatc atgccagagg ggctaccata cttgcaagaa
    13441 ccaaacaatg ccaacttgac agaaggtgtt ccagctaaat acttcttgcc acttggtgga
    13501 gatatcaagg aagctatcag caatcttgag ctcaaccctc cattcaagaa gaaaaacctt
    13561 ggaaatatca tcgctgaaat cttcaaacgt ttccgtacga cagaaacttc tgccctactt
    13621 gaccgcatga agaacctcgg ttaccaccac tcaactcttg caggattgac agtgggtatt
    13681 gccgatatcc cagtcgttga tgacaaggct gaaatcattg aagaatcaca caaacgtgtc
    13741 gaacaaatca ccaaacaatt ccgtcgtggt atgatcacag acgacgagcg ttacaatgct
    13801 gttacagctg aatggcgtgc tgcccgtgaa aaacttgaga aacgcttgat tgctaaccaa
    13861 gatcctaaga acccaatcgt tatgatgatg gactctggag cccgtggtaa catctcaaac
    13921 ttctcacagc ttgccggtat gcgtggtctg atggctgctc cgaatggacg tatcatggaa
    13981 ttgccaatcc tttcaaactt ccgcgaaggt ttgtcggtat tggaaatgtt cttctcaact
    14041 cacggtgctc gtaaaggtat gaccgatacg gcccttaaga cagccgactc aggttacttg
    14101 actcgtcgtt tggtcgacgt tgcccaagac gttatcatcc gtgaggacga ctgtggaact
    14161 gaccgtggtc tcttgatccg ttctatcgca gaaggaaaag agatgatcga gtctctcgaa
    14221 gagcgtctca acggtcgtta cactaagaaa actgttaaac atccagaaac tggtgcagtg
    14281 attattggtc caaatgaatt gattacagaa gacaaggcgc gtgaaattgt caatgctggt
    14341 gtggaagaag tgactatccg ttctgtattt acatgtaaca ctcgtcacgg tgtctgccgt
    14401 cactgttatg gtatcaactt ggcgactggt gatgcggttg aagttggtga agcagttggt
    14461 acaatcgctg cccaatctat cggggaacct ggtacacagc ttacaatgcg taccttccac
    14521 acaggtgggg ttgcctcaaa taccgatatc actcagggtc ttcctcgtgt ccaagaaatc
    14581 tttgaagccc gcaatcctaa aggggaagcg gttatcacag aggttaaagg acaagttact
    14641 gctatcgaag aagatgcatc aactcgtacc aagaaagtct ttgttaaggg tgaaactggc
    14701 gaaggtgaat atgtcgttcc atttacagct cgtatgcgtg tcgaagttgg gggccaagta
    14761 gcgcgtggtg ctgctctgac agaaggttct atccaaccaa aacgtctcct tgcagttcgt
    14821 gatgtcttgt cagttgaaac gtaccttctc ggtgaagtac aaaaagttta ccgtagccaa
    14881 ggggtagaaa tcggtgacaa acacatcgag gtaatggttc gtcaaatgat ccgtaaagtc
    14941 cgtgtcatgg atccaggtga tacagatctt ctcatgggta ccctcatgga tatcaatgac
    15001 tttacagatg ccaacaaaga tgtccttatc gcaggtggag ttccagcgac aggtcgccca
    15061 gtccttatgg gaattaccaa agcctcactt gaaacaaaca gtttcttgtc agcggcttcc
    15121 ttccaggaaa caactcgtgt ccttactgac gcagctatcc gtggtaagaa agaccatctc
    15181 cttggactta aagaaaatgt tatcatcggt aagatcatcc cagctggtac tggtatggcc
    15241 cgttaccgta accttgaacc acatgctgtc aacgaagaag aataccttaa tcctccagta
    15301 gaggaagaag gaaatgaaga aacaacagaa gtagttgtgg atactgccgt tgaaactgtg
    15361 gaagaaacag tagaataaga gagaatgaga gaaccctcgg gttctctcat tctctcttct
    15421 gaaaactttc tccatttttc cacgaaatgt ggtaaaataa aagaaagaag ctgacaaagg
    15481 agacgggtat ggaacaaaca ttctttatca tcaaaccaga tggtgtaaaa agagggctag
    15541 tgggtgaagt gttaaagcgc atcgaacaac gtggatttac aatcgaaaaa ttggagtttc
    15601 gttcacaggt ttcagaagag ttgattgacc agcactatca ggacttggtt ggtcagagtt
    15661 tttacccacc gattcgtgaa ttcatgactt caggtccagt tcttgtgggt gtcatttctg
    15721 gtcccaaagt aatcgaaact tggcggacca tgatgggtgc aactcgtcca gaagaagctt
    15781 taccaggcac tattcgaggt gattttgcaa aagctgctgg agaaaatgag attatccaaa
    15841 atgttgtaca tggttcagat tcagaagagt cagctaagcg agaaattgct ctttggtttt
    15901 aagagtggat tggctcaatc aattggttaa aagctcattt gaatagaaag tatagtcaat
    15961 tagtttaaga catgacgcat gatatcaaac tttttagttt ttgatatggt gcgttttttg
    16021 attaagtgac tattagtttg tctcgctccg ttaggtgcga gacaaaaaaa acacccgcta
    16081 tgcgggtgcg cgtcgaaggt tataccaaaa aaactccaaa cgcgatacaa taaaggtgtt
    16141 caatccaatt gtaaagcgaa aggagaaaaa tatggcacaa aaggttcttt tatcctgttc
    16201 ttgtttcaat tgactatatt tctatacttt ttcttccttt ctctgtgaaa atagcaagtt
    16261 tttcccacct tttctcaata ctgaaacaac atctttccag aacttcaata atttcttttt
    16321 gtgttaaatc attatttaac taaatctttg gaggcaatac gttttcaaag ctactttttt
    16381 accatagatt ttgagccttc aacacagatt actaagccaa aaatgctcta aatttttgaa
    16441 attctaacaa attccactca actttatcat catctttgac tttcacaata tcttcctcaa
    16501 aataacatgg aataaagata gttttctttt aaaacggttg taggaggaaa atctctccta
    16561 cactattatt gattctgaga gaaaattgca aaatatcatc taccaaactt tcaattatta
    16621 agattttttg cagtttatct gaattagaat cgcaaggctt ctcaataata taattgtcga
    16681 attttttcat tctaacttcc tctccattat ttcttccttt at
//
LOCUS       DAVMEF010000031        16456 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMEF010000031 DAVMEF010000000
VERSION     DAVMEF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.031"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..497
                     /locus_tag="VYQ30_000708"
     CDS             24..497
                     /locus_tag="VYQ30_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1965863.1"
                     /translation="MKKYTEVFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGN
                     TEKYIATYVNQEFKSYIDHMLNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNG
                     LGLFSQFLISVHYDSWNDKANKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            737..1015
                     /locus_tag="VYQ30_000709"
     CDS             737..1015
                     /locus_tag="VYQ30_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEV1965864.1"
                     /translation="MEKVASLGVPMCKPISIELCDDEVHSLHEWIDGRDAIDSILTYS
                     ENQQYTYGVEAGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            1120..1449
                     /locus_tag="VYQ30_000710"
                     /pseudo
     CDS             1120..1449
                     /locus_tag="VYQ30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1577..2806
                     /locus_tag="VYQ30_000711"
     CDS             1577..2806
                     /locus_tag="VYQ30_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1965865.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2826..5522
                     /gene="ppc"
                     /locus_tag="VYQ30_000712"
     CDS             2826..5522
                     /gene="ppc"
                     /locus_tag="VYQ30_000712"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1965866.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5605..5822
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5935..6939
                     /locus_tag="VYQ30_000713"
     CDS             5935..6939
                     /locus_tag="VYQ30_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1965867.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPEFVLKEAGQVIE
                     "
     gene            7008..7874
                     /locus_tag="VYQ30_000714"
     CDS             7008..7874
                     /locus_tag="VYQ30_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1965868.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7871..8629
                     /locus_tag="VYQ30_000715"
     CDS             7871..8629
                     /locus_tag="VYQ30_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965869.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            8719..10479
                     /gene="dnaG"
                     /locus_tag="VYQ30_000716"
     CDS             8719..10479
                     /gene="dnaG"
                     /locus_tag="VYQ30_000716"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1965870.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10482..11591
                     /gene="rpoD"
                     /locus_tag="VYQ30_000717"
     CDS             10482..11591
                     /gene="rpoD"
                     /locus_tag="VYQ30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1965871.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLSNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11605..11934
                     /locus_tag="VYQ30_000718"
     CDS             11605..11934
                     /locus_tag="VYQ30_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1965872.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12049..13092
                     /gene="cpoA"
                     /locus_tag="VYQ30_000719"
     CDS             12049..13092
                     /gene="cpoA"
                     /locus_tag="VYQ30_000719"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1965873.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPATLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13108..14433
                     /locus_tag="VYQ30_000720"
     CDS             13108..14433
                     /locus_tag="VYQ30_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1965874.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAISEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14464..14640
                     /locus_tag="VYQ30_000721"
     CDS             14464..14640
                     /locus_tag="VYQ30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1965875.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14699..14833
                     /locus_tag="VYQ30_000722"
     CDS             14699..14833
                     /locus_tag="VYQ30_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1965876.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14897..16207
                     /gene="obgE"
                     /locus_tag="VYQ30_000723"
     CDS             14897..16207
                     /gene="obgE"
                     /locus_tag="VYQ30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1965877.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVHDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEETYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16217..16375
                     /locus_tag="VYQ30_000724"
     CDS             16217..16375
                     /locus_tag="VYQ30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965878.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5096 a         2831 c         3675 g         4854 t
ORIGIN      
        1 ttcgagaaaa ttgggaaccg tacatgaaaa agtacacgga agtttttcaa agtcaatttc
       61 ctaatctaaa cattgattac ttgctcctgg acactgagca gattgatctt gatagctatc
      121 tagatgctga cataattatc atcggtggtg gaaatacgga aaaatatatt gctacttatg
      181 ttaatcagga gttcaaaagt tatatcgatc atatgcttaa taaagaggca aaaattatag
      241 gattttctgc aggagcccta ctattaggag aaaaagtcta tgtctcacct aatgataatt
      301 cagatcatca gataaagata aaaaatggat taggactctt tagtcagttt ttaattagtg
      361 tccattatga ttcctggaat gataaagcaa ataaggatag agctgaagaa ctcgttaatg
      421 ttcccataat tccactaaat gatcattctt gtcttgtatt ggataaactt ggaaacatca
      481 tagagaaaat tgactaactt tcataatgta aaaatggatc taggaaataa aagtgtagac
      541 gaactggata ggttactagt gaaaactgca aaaagtgttg taaagtgaat cggagtgttt
      601 tagtagtaag atagccataa ataaaggctg gtcagatgat aaaaaatatt gtgtgacagt
      661 tcaaaatcag taaacatatt tcttgcgtgt ttctgataag tcgaagttag attataaaaa
      721 aattgaattt gatacgatgg agaaagtagc ttctcttgga gttccgatgt gtaaaccaat
      781 tagcattgaa ctctgcgatg acgaagtaca ttctttacac gaatggatag acggaagaga
      841 tgcaatagat agcattttaa cttattcaga aaaccaacaa tacacatatg gtgtagaagc
      901 aggaaagata cttagaaaga ttcatacaat tcctgctaca gaagtttgtg aagattggga
      961 aatctttttt aatctaaaaa ttgatgataa aatctccaac gaaatgatat ggtaagtccg
     1021 aaacgctcag tgtgtaagct tgttgctaaa agcgtaaaaa ccttggaacg aaaggaataa
     1081 tagacttcct gcgaaacaaa aatacaatac aataaaacta tggatgatga agcaagtaaa
     1141 caattgagcg atagccgttt caagatcctt gtaggtgttc agcgcacgac ttttgaagag
     1201 atgttagctg tgttaaaaac agcttatcaa cgtaaacgcg caaaaggtgg acgaaaaacc
     1261 aaattaagcc tagacgatct ccttatggta actattcaat acatgcgaga atagcgcact
     1321 tatgaacaaa tttcggctga ttttggcatt cacgaaagca acttaatccg tcggagtcaa
     1381 tgggttgaag taactcttgt tcaaaatggt tttacgattt caaattctgc tctaattatg
     1441 aaaaacagta aaattcgaag gattataagg taagagtttt tttctttctg aaaaaatggt
     1501 ataatagcaa tcaaaactag aaaataaaac ggaatttgga acagatttgt ctgtatccta
     1561 gtagagtggt gatgctatga agattagtaa gaggcactta ttaaattatt ccatcttgat
     1621 tccctacttg cttttatcta ttttgggctt gattgtggtc tattcgacca ccagtgctat
     1681 tttaattgaa gaaggcaaga gtgccttgca gttggttcga aaccaaggaa tcttttggat
     1741 tgttagtttg atactgattg ccttaattta taaattgaga ctagattttt tgagaaatga
     1801 gcgactaatc attttagtta tattaataga aatgctttta ttgttcttgg ctcgttttat
     1861 tggtatttca gtaaacgggg catacggttg gatttcggtt gcaggagtaa ctattcagcc
     1921 agctgagtac ttaaaaatca ttattatttg gtatttagct caccgattct ccaaacagca
     1981 agaagaaata gctacttatg attttcaagt tttgactcaa aatcaatggc ttccccgtgc
     2041 ttttaatgat tggcgattcg ttctcctagt tctgattgga agtttgggaa ttttccctga
     2101 tttaggaaat gcgactattt tagtcttggt ttccttgatt atgtatacag ttagtggaat
     2161 cgcttatcgc tggttttcaa ccattctggc gctcgtatct gccgcttctg tctttgtctt
     2221 gaccactatc agcctaatcg gtgttgagac cttttcaaaa attccagtat tcggctatgt
     2281 agccaagcgc tttagtgcct tttttaatcc ttttgccgat cgtgctgatg caggtcacca
     2341 gttagctaat tcttattttg ccatggtcaa tggcggttgg tttggtctag gtcttggaaa
     2401 ctcgattgaa aaacgaggtt atttgccaga agctcataca gactttgtct tttctatcgt
     2461 gattgaagaa tttggctttg ttggtgccag tcttatttta gctctcttgt ttttcatgat
     2521 tttgcggatt atcttggtcg gtatccgagc ggagaatcct ttcaatgcca tggttgcact
     2581 cggtgtcgga gggatgatgt tggttcaggt atttgtcaat atcggaggga tttcgggctt
     2641 gattccatct acaggagtga ctttcccctt cttatcccag ggtggaaata gtcttctagt
     2701 cttatcagtg gcagtagcct ttgtcttaaa tattgatgcc agtgaaaaac gcgctaaatt
     2761 gtaccgagaa ttggaaaatc aaccaatgaa ccttctgttg aagtaggata aagaaaggat
     2821 agtttatgtc tcttcaaaaa ttagaaaatt atagtaataa aagtgttgtg caagaagaag
     2881 tcttgattct aacagaatta ctggaagata ttactaaaaa tatgcttgcc ccagagacct
     2941 ttgaaaaaat aatacagttg aaagaattat caacgcagga agattatcaa ggtctaaacc
     3001 gtctagtgac tagcttatca aatgatgaaa tggtctatat ttcacgctat ttctctatct
     3061 tgcctctttt gattaatatt tcagaggatg tggatttagc ttatgaaatc aatcatcaaa
     3121 ataatattga tcaggactat ttaggtaaat tatctacaac gattaaattg gtagcagaaa
     3181 aggaaaatgc cgttgagatc ctagaacact tgaatgttgt ccctgttttg acagcccatc
     3241 caacacaagt gcaacgcaaa agtatgttgg atttaacaaa tcatattcat agtcttttgc
     3301 gtaaataccg tgatgttaag ttggggttga tcaataaaga taaatggtac aatgatttgc
     3361 gtcgttacat cgaaattatc atgcagacag acatgattcg tgagaaaaaa ttaaaagtga
     3421 ctaacgaaat cacgaatgct atggaatatt ataacagctc ctttttgaaa gctgtacctc
     3481 atttgacgac ggagtataag cgcttagcgc aagcgcatgg tctgaattta aaacaggcta
     3541 aaccaatcac catgggtatg tggataggtg gtgaccgtga tggaaatcca tttgttacag
     3601 caaagacctt gaagcagtct gcactcactc agtgtgaagt catcatgaac tactatgata
     3661 aaaagattta ccaactttat cgtgaatttt ctctttcaac tagcattgtc aacgtcagca
     3721 agcaagtcag agaaatggct cgtcaatcca aggataactc gatttaccgc gaaaaagagc
     3781 tttaccgtcg tgccttgttt gatattcaat caaaaattca ggcaactaaa acctatctga
     3841 ttgaggatga agaagttggg actcgttatg aaaccgccaa tgatttctac aaggatttga
     3901 ttgccattcg agattctcta ctagaaaata agggcgagtc cttgatttca ggtgattttg
     3961 tggaattatt gcaggcagta gagatatttg gtttttactt agcatcaatt gatatgcgac
     4021 aagactctag cgtctatgaa gcctgtgtgg cagaactctt gaaatcagca ggaattcatt
     4081 ctcgttatag cgagttgagc gaagaagaaa agtgtgacct tctcttgaaa gaattagaag
     4141 aagatccccg aattctttct gcgactcacg cagaaaaatc agaattatta gcaaaagaac
     4201 tagctatttt taagacggct cgtgttttga aagataagtt gggagatgat gtcatccgtc
     4261 agaccatcat ttcacatgca accagccttt ctgatatgct agaattagct attctgttaa
     4321 aagaagtagg actggtggat acggaaaggg cgcgtgttca gattgttccc ctttttgaaa
     4381 caattgaaga cttggatcat tcagaggaaa caatgagaaa atatctttct cttagccttg
     4441 ccaaaaaatg gattgactca cgaaataact accaagaaat catgcttggc tactctgaca
     4501 gtaataaaga tggcggttac ttgtcatcat gttggaccct ctacaaggct caacaacaat
     4561 tgactgctat tggagatgaa tttggcgtta aggttacctt cttccatggt cgtggtggta
     4621 ctgtcggtcg tggtggtggg ccaacctatg aagccattac atctcaaccg ctcaagtcta
     4681 tcaaggatcg tatccgcttg acggagcagg gtgaagtaat tgggaataaa tacggtaaca
     4741 aagacgccgc ttactataac cttgaaatgc tagtatcggc agctattaac cgtatgatta
     4801 ctcagaagaa gagcgatacc aataccccaa atcgttatga agccattatg gatcaagtag
     4861 tggaccgtag ttacgatatc taccgtgatt tggtctttgg taatgagcat ttctatgatt
     4921 atttcttcga gtcaagtcca atcaaggcta tttcaagttt taatattggt tctcgtccag
     4981 ccgctcgtaa gactattact gaaatcggtg gattgcgtgc tatcccttgg gtattctcat
     5041 ggtcacagag tcgtgttatg ttccctggat ggtacggggt tggttcaagc ttcaaggaat
     5101 ttatcaataa aaatccagag aatattgcta tcttacgaga tatgtaccaa aattggcctt
     5161 tcttccaatc gcttctttca aatgttgata tggttttgtc aaaatcaaat atgaatattg
     5221 cttttgaata tgctaaactt tgtgaagacg agcaagttaa ggccatctat gagactattt
     5281 taaatgaatg gcaagttact aagaacgtta tcttggctat tgaaggacat gacgaactct
     5341 tagctgacaa tccatatcta aaagctagtc tggattaccg tatgccttac tttaatattc
     5401 tcaactatat tcagttagag ttgattaaac gccaacgtcg tggagaattg tccagtgatc
     5461 aagaacgatt gattcatatc accatcaacg gaattgcgac aggattgcgt aattcaggtt
     5521 gataattttc aagagtgaat gctaaaagtg aatatcaaaa aaattctaat agactattga
     5581 caagtagttt aaaaatgata taatttaacc attcagaaaa gtaatcatac aaacttttta
     5641 gagagtctgt ggtagctgaa aacagataag tggcaatgat gaaaattggg ctgaatgcta
     5701 tttagaattt gaaattataa aaattcggta agcacacctt acagtgcatc tcgttattgc
     5761 gagactgagc gatagggaaa ttccctataa ttgaggtggt accgcgcatc gacgtcctca
     5821 cacaagtttt ttgtgtgagg atttttttga tggaggttag tatagaaaga aaacgatggc
     5881 gtcgcttgtt tagataagtg aaatatgtta aaggaaataa aaaggagaaa cagaatgaaa
     5941 aataaacgtt taattggaat tattgctgca ttagcagtct tagtagcagg aagcttgatt
     6001 tattcttcaa tgaataaatc agaagctcag aataataagg atgagaagaa aataaccaag
     6061 attggtgtgc ttcaatttgt gagccatcca tcccttgatt tgatttataa agggatccaa
     6121 gatggacttg cagaagaagg atataaagat gatcaagtta aaattgattt tatgaactca
     6181 gaaggtgacc aaagtaaggt tgcgacaatg agtaaacaat tggttgcaaa tgggaatgac
     6241 cttgtggttg gtatcgcaac accagcagcc caagggttgg ctagtgcaac aaaagaccta
     6301 ccggttatca tggccgctat tacagaccca attggtgcta acttggttaa agatttgaaa
     6361 aaaccaggtg gcaacattac aggggtatct gaccacaatc cagctcaaca acaagttgaa
     6421 ctcatcaagg ctctgacacc gaatgtgaaa acaatcggag ctctttactc aagtagcgaa
     6481 gacaattcaa aaacacaggt cgaagaattt aaggcttatg ctgaaaaagc aggtctgaca
     6541 gtggaaacat ttgcagttcc ttcaacaaat gaaattgcct caactgtcac tgttatgact
     6601 agcaaggtag atgctatttg ggttccaatt gataacacca ttgcatcagg atttccaacg
     6661 gttgtctcta gcaatcaaag ttctaagaaa ccaatttatc ccagtgcgac agctatggta
     6721 gaagtaggtg gtttggcatc agttgtaatt gaccaacatg accttggtgt ggcaacaggt
     6781 aaaatgattg tgcaagtctt gaaaggtgca aaaccagccg ataccccagt caatgtcttt
     6841 tcaactggta agtcagtcat caataaaaaa atagcacaag aactaggtat tactattcct
     6901 gagtttgttc tcaaagaagc aggacaagtc atcgaataaa taaggtaggg aggaggagac
     6961 tcttcccatt ttttaaaaag atgaatatag aaaggattaa ataaaatatg attgtttcca
     7021 ttatttctca aggatttgtc tgggctattc taggtctggg aatctttatg acatttagga
     7081 ttttaaactt tccagatatg acgacagaag gttccttccc tcttggggga gctgttgctg
     7141 tcactttgat aaccaaaggc gtgaacccat ttttagcgac acttgttgct gtaggagcag
     7201 gttgtttggc tggaatggca gcaggccttc tttatacaaa agggaagatc ccaaccttgc
     7261 tctcagggat tttggtgatg acttcttgtc actcaatcat gctcttgatt atgggacgtg
     7321 caaatttagg cctgcttgga accaagcaaa ttcaggatgt tttgcctttt gattcggatt
     7381 tgaatcaact cttgacaggt ctcatctttg tgagtattgt tattgctctc atgctctttt
     7441 tcttggacac taaactcgga caagcctata ttgctacagg ggataatcct gatatggcta
     7501 gaagtttcgg gattcatact ggacacatgg agctcatggg cttggtctta tcaaatggtg
     7561 tgattgccct tgcaggtgcc ctcattgctc agcaagaagg ttatgccgat gtgtctcgag
     7621 ggatcggggt tatcgttgtg gggcttgcaa gtttgattat tggagaagtt attttcaaga
     7681 gtttgagctt ggcagagcgt ttggttacta tcgttgtagg ttctatcgct tatcaatttt
     7741 tagtgtgggc agttatcgca cttggcttta atacaagtta ccttcgttta tacagtgcct
     7801 tgattttagc agtctgcctc atgattccaa catttaagca aacaatcttg aaaggagcca
     7861 agttaagcaa atgacagcaa ttgtagaatt aaaaaatgca accaaaatcg ttaaaaatgg
     7921 ctttgatgaa gaaaagatta ttttaaatga tgtttcctta gaaatttttg aacaggactt
     7981 tatcacgatt ttgggtggaa atggtgctgg taaatcaacc ctctttaaca ctattgcagg
     8041 aaccatatca ctaaccagtg gaactatccg tattttaggt gaggatgtta ccaagttttc
     8101 accagagaag cgcgctaagt atctgtctcg agtcttccaa gatccaaaga tggggacagc
     8161 tcctcgtatg acagtggcag agaatctcct gattgccaag tttcgtggtg aaaagcgtgg
     8221 actatttccg agacgtctgg ctagctataa ggataagttt caggcgacca ttgaaaaagt
     8281 gggaaacggt cttgagaaac acttgaatac accgattgag ttcttatctg gtggacaaag
     8341 acaggctttg agtctcttga tggcaacctt gaagcgacct gaattactct tgttagacga
     8401 gcatactgct gccctggatc caaagactag tgttgctttg atggaattga cagatgaatt
     8461 tgttaagaaa gatcaattga cagcccttat gattacccat catatggaag atgctctcaa
     8521 atatggcaat cgcttgattg tcatgaaaga aggacgggtt atccaagatt tgaaacaaga
     8581 agaaaaagca aaaatgaaaa tctctgatta ttatcaactc tttgaataaa gtagggaaaa
     8641 tgagtgaaat ggtttacttt ttttctgaaa taaagtatac tatataaagt aaactatgat
     8701 aacatggagg tattgtgtat ggttgacaaa caagtcattg aagaaatcaa aaacaatgcc
     8761 aacattgtgg aagtcatagg agatgtgatt tctttacaaa aggcaggacg gaactatcta
     8821 gggctctgtc cttttcatgg tgaaaaaaca ccttctttca acgttgtaga ggacaagcag
     8881 ttttaccact gttttggttg tggtcgctca ggtgatgtct ttaaattcat cgaggagtac
     8941 caaggggtta cctttatgga ggctgtccaa atcttaggtc agcgtgtcgg gattgaggtt
     9001 gaaaaaccgc tttatagtga acagaagcca gcctcgcctt accaagctct ttatgatatg
     9061 cacgaagatg cggctaaatt ttaccatgct attctcatga caacgactat gggcgaagag
     9121 gccagaaatt acctttatca gcggggtttg acagatgaag tgcttaaaca tttttggatt
     9181 ggtttagcac ctccagaacg aaactatctc tatcaacgtt tgtctgatca gtatcgtgaa
     9241 gaggatttac tggattcagg cctgttttat ctctcggatg ccaatcaatt tgtagacacc
     9301 tttcacaatc gcattatgtt tcccctgaca aatgaccaag gaaaggtcat tgccttctca
     9361 ggtcgtatct ggcaaaaaac ggattcacaa acttctaagt ataaaaacag ccgatcgact
     9421 gcaattttta acaaaagtta cgaattatat catatggata gggcaaaaaa atcttctgga
     9481 aaaactagtg agatttacct gatggaagga ttcatggatg ttattgcagc ctatcgggct
     9541 ggaatcgaaa atgctgtggc gtcgatggga acggccttga gtcgagagca tgttgagcat
     9601 ctgaaaaggt taaccaagaa attggttctt gtttacgatg gagataaggc tgggcaagcc
     9661 gcgatattga aagcattgga tgaaattggt gatatgcctg tgcaaatcgt tagcatgcct
     9721 gataacttgg atcctgatga gtatctacaa aaaaatggtc cagaagactt ggcctatcta
     9781 ttaacaaaaa ctcgtattag tccgattgag ttctacattc atcagtacaa acctgaaaac
     9841 agtgaaaatc tgcaggctca gattgagttt attgaaaaaa tagctccttt gattgttcaa
     9901 gaaaagtcca tcgctgctca aaacagctat attcatattt tagctgacag tctggcgtcc
     9961 tttgattata cccagattga gcagattgtt aatgagagtc gtcaggtgca aaggcagaat
    10021 cgcatggaaa gaatttccag accgacgcca atcaccatgc ctgtcaccaa gcagttatcg
    10081 gctattatga gggcagaagc ccatctactc tatcggatga tggaatcccc tcttgttttg
    10141 aacgattacc gtttgcgaga agactttgca tttgctacac ctgaatttca ggtcttatat
    10201 gacttgcttg gccagtatgg aaatcttcct ccagaagttt tagcagagca gacagaggaa
    10261 gttgaaagag cttggtacca agttttagct caggatttgc ctgctgagat atcgccgcag
    10321 gaacttagtg aagtagagat gactcgaaac aaggctctct tgaatcagga caatatgaga
    10381 atcaaaaaga aggtgcagga agctagccat gtaggagata cagatacagc cctagaagaa
    10441 ttggaacgtt taatttccca aaagagaaga atggagtaat aatggcaaca aaacaaaaag
    10501 aagtaacaac atttgacgta caggtagcag aatttatccg taatcataag caaaaaggga
    10561 cagcaacaga tgatgaaatc aatgcaagtt tggttattcc ttttaccttg gacgctgatg
    10621 ggattgaaga tctcttgcaa cggattcagg atgcaggaat ttctatcaca gataacgaag
    10681 gaaatccaag tgcgcgtgtt cttagcaatg aagaagaacc agaactcagc gatgaggact
    10741 tgattgggtc aacttctgct aaggtcaatg accctgtccg tatgtacttg aaagaaatag
    10801 gggtcgttcc tctcttgagc aatgaagagg agaaagagtt ggcactggct gttgaagctg
    10861 gtgatatcga agccaaacaa cgtcttgcgg aagccaatct tcgtttggtt gtttccattg
    10921 ccaaacgcta tgtcggtcgt ggtatgcagt tccttgactt gattcaagaa ggaaatatgg
    10981 gcttgatgaa ggcggttgac aagtttgact attctaaagg gttcaagttt tcaacttatg
    11041 caacttggtg gattcgtcag gctatcactc gtgctattgc ggaccaagct cgtaccatcc
    11101 gtatcccagt tcacatggtt gaaactatca ataaattggt tcgtgaacag cggaatctcc
    11161 ttcaagaatt ggggcaagat ccgacaccag aacagattgc tgaacgaatg gatatgacac
    11221 ctgataaggt tcgtgaaatc ttgaagattg cccaagaacc agtatctctt gaaactccta
    11281 tcggtgaaga ggacgatagc caccttggag actttatcga agatgaagtg attgaaaatc
    11341 cagtggatta tacgactcgt atcgtcttgc gtgagcaatt ggatgaaatc ttagatactc
    11401 ttacagaccg tgaagaaaat gttctgcgtc tacgttttgg actagatgat ggaaaaatgc
    11461 gcacacttga agatgtgggg aaagtcttta acgtaactcg tgagcgtatc cgtcagattg
    11521 aagcaaaggc tttgagaaaa ctacgccaac caagtcgtag caaaccgctt cgtgatttta
    11581 ttgaagacta agagtgagga aaatatggct tatacagaag agcaaattga aaacatcaaa
    11641 acacggattt taacagcctt ggaagaagtc atcgaccctg agttgggaat cgatattgtc
    11701 aatcttggtt tgatctatga gattcgtttt gacggtgaca cagggcaaac agagattgac
    11761 atgactttga caactatggg ttgtccctta gcagaccttt tgacagacca gatttatgat
    11821 gctatgatag aggttccaga agtaacggat actgaggtta aattagtctg gtatccagcc
    11881 tggacagttg aaaaaatgag tcgctatgcc cgcattgccc taggcattaa gtaaacatat
    11941 aaaagcatgt gagagactgt tggaaaagcg aggaaatttc ccctcttttc ctctagtctc
    12001 tcctttcttt tgctgatttt attcaaagaa aatgatataa tagtagttat ggagaaaaag
    12061 aaattacgca tcaatatgtt gagttcaagt gagaaagtag caggacaggg agtttcaggt
    12121 gcttaccgtg aattagttcg tcttcttcac cgtgctgcca aggaccaatt gattgttaca
    12181 gaaaatcttc caatcgaggc agatgtgact cactttcata cgattgattt tccctattat
    12241 ttatcaacct tccaaaagaa acgctcaggg agaaagattg gctatgtgca tttcttgcca
    12301 gctacacttg agggaagttt gaaaattcca tttttcttaa agggaattgt gaaacgctat
    12361 gtattttctt tttacaaccg gatggagcac ttggttgtgg tcaatcctat gtttattgag
    12421 gatttggtag cagctggtat tccacgtgaa aaagtgacct atattcctaa ctttgtcaac
    12481 aaggaaaaat ggcatcctct accacaagaa gaggtagtta gactgcgcac agatcttggt
    12541 cttagtgaca atcagtttat cgtagtaggt gctgggcaag ttcagaaacg taaagggatt
    12601 gatgacttta tccgtctggc tgaggaattg cctcagatta cctttatctg ggctggtggc
    12661 ttctcttttg gtggtatgac agatggttat gaacactata agaaaattat ggaaaatccc
    12721 cctaaaaatt tgatttttcc aggcattgta tcgccagagc ggatgcgcga attgtatgct
    12781 ctagcggatc ttttcttgtt gcctagttac aatgagctct ttcctatgac tattttagaa
    12841 gctgcgagtt gtgaggctcc tattatgttg cgtgatttag atctctataa ggtgattttg
    12901 gagggaaatt atcgggcgac agcgggtaga gaagagatga aagaggctat tttggaatat
    12961 caagcaaatc ctgctacctt aaaagatctc aaagaaaagg ctaagaatat ttccagagag
    13021 tattctgaag agcatctgtt acaaatctgg ttggactttt atgagaaaca agccgcttta
    13081 gggagaaagt aaaaagtgag gtaatctatg cgaattggtt tatttacaga tacctatttt
    13141 cctcaggttt ctggtgttgc gaccagtatt cgaaccttga aaacagaact tgaaaagcag
    13201 ggacatgctg tttttatctt tacgacgaca gataaggatg tcaatcgcta cgaagattgg
    13261 caaattatcc gcattccaag tgttcctttc tttgctttta aggatcgtcg ctttgcctac
    13321 cgaggtttta gcaaggcact tgaaattgct aaacagtatc agctagatat tatccatact
    13381 cagacagaat tttctcttgg cctgttgggg atttggattg cgcgtgaatt gaaaattcca
    13441 gtcatccata cctatcacac ccagtatgaa gactatgtcc attatattgc taaggggatg
    13501 ttgatccggc cgagtatggt caagtatctg gttagaggtt tcctgcatga tgtggatggg
    13561 gttatttgcc ctagtgagat tgtccgtgac ttgctatctg attataaggt caaggttgaa
    13621 aaacgggtca ttcctactgg aattgaatta gccaagtttg agcgtccgga aatcaagcag
    13681 gaaaatttga aagaactgcg tagtaaacta gggattcaag atggtgaaaa gacgttgctt
    13741 agtctttcga gaatctccta tgaaaaaaat attcaagcag ttttagtagc ctttgctgat
    13801 gttctgaaag aggaagacaa ggttaaactg gtagtagctg gggatggccc ttatctgaat
    13861 gacctcaaag agcaagccca gaacctagag attcaagact cagtcatctt tacagggatg
    13921 attgctccta gtgagacggc tctttactat aaagcggcgg atttcttcat ttcggcatcg
    13981 acaagcgaaa cgcaaggttt gacctacttg gaaagcttag ccagtggaac acctgtcatt
    14041 gctcacggaa atccttattt gaacaacctc atcagtgata aaatgtttgg aaccttgtac
    14101 tatggagaac atgatttggc tggtgctatt tcggaagccc tgattgcaac accagacatg
    14161 aatgagcata ccttatcaga gaaattgtat gagatttcag ctgagaactt tgggaaacga
    14221 gtgcatgagt tttatctgga tgccattatt tcaaataact tccagaaaga tttggctaaa
    14281 gatgatacgg tcagtcagcg tatctttaag acagttttgt atcttccgca acaggtggtt
    14341 gctgtacctg taaaaggatc tagacgcatg ttgaaggctt caaaaacaca gttgatcagt
    14401 atgagagact attggaaaga ccatgaagaa tagaaagagg aacagctatg aaaaaaacaa
    14461 ttaatgagaa gcggtcgtga taaaaagatt gcgggtgttt gtgctggggt ggcccattat
    14521 ctggatatgg atccgactat cgttcaagtc atttggggtg ttcttacttg ctgttacgga
    14581 gctggaattg tagcttacat tattttatgg attatcgcac cagtagcaac tgactattga
    14641 aaactagctc aattcatgct aagataatag aaaatagaat gtaacgaagg agagaaaaat
    14701 ggcatttgga gataatggaa atcgtaaaaa aactatgttt gagaaaataa ccttgtttat
    14761 cgtgattatc atgctagtag caagtttatt gggaattttt gcaactgcaa ttggtgccct
    14821 cagtaatcta taaaatagat tcaagaaaat ttagtgactg ggatttccca gccctttttt
    14881 aaagtgagaa gaaataatga gtatgttttt agatacagct aagattaagg tcaaggctgg
    14941 taatggtggc gatggtatgg ttgcctttcg tcgtgaaaaa tatgtcccta atggaggccc
    15001 ttggggtggt gatggtggtc gtggaggcaa tgtggtcttc gttgtagacg aaggactacg
    15061 taccttgatg gattttcgct acaatcgtca tttcaaggct gattctggtg aaaaagggat
    15121 gaccaaaggg atgcatggtc gtggtgctga ggaccttaga gttcgagtac cacaaggtac
    15181 gactgttcat gatgcggaga ctggcaaggt tttaacagat ttgattgaac atgggcaaga
    15241 atttatcgtt gcccacggtg gtcgtggtgg acgtggaaat attcgtttcg cgacaccaaa
    15301 aaatcctgca ccggaaatct ctgaaaatgg agaaccaggt caggaacgtg agttacaatt
    15361 ggaactaaaa atcttggcag atgtcggttt agtaggattc ccatctgtag ggaagtcaac
    15421 acttttaagt gttattacct cagctaagcc taaaattggt gcctaccact ttaccactat
    15481 tgtaccaaat ttaggtatgg ttcgcaccca atcaggtgaa tcctttgcag tagccgactt
    15541 gccaggtttg attgaagggg ctagtcaagg tgttggtttg ggaactcagt tcctccgtca
    15601 catcgagcgt acacgtgtta tccttcacat cattgatatg tcagctagcg aaggccgtga
    15661 tccatatgag gattacttag ctatcaataa agagctggag tcttacaatc ttcgcctcat
    15721 ggagcgtcca cagattattg tagctaataa gatggacatg cctgagagtc aggaaaatct
    15781 tgaagaattt aagaaaaaat tggctgaaaa ttatgatgaa tttgaagagt taccagctat
    15841 cttcccaatt tctggattga ccaagcaagg tctggcaaca cttttagatg ctacagctga
    15901 attgttagac aagacaccag aatttttgct ctacgacgag tccgatatgg aagaagaaac
    15961 ttactatgga tttgacgaag aagaaaaagc ctttgaaatt agtcgtgatg acgatgcgac
    16021 atgggtactt tctggtgaaa aactcatgaa actctttaat atgaccaact ttgatcgtga
    16081 tgaatctgtc atgaaatttg cccgtcagct tcgtggtatg ggggttgatg aagcccttcg
    16141 tgcgcgtgga gctaaagatg gggatttggt ccgcattggt aaatttgagt ttgaatttgt
    16201 agactaggag actggtatgg gagataaacc gatatctttc cgagatgcgg atggtaattt
    16261 tgtttccgcc gcagacgttt ggaatgaaaa gaaattggaa gaactattta atcgtctcaa
    16321 tccaaatcgt gccttgagat tggcacgaac taaaaaggaa aatccatctc agtaaagaag
    16381 ctaaaaaatc ccgtgcctca tcagacacgg gattttgtgg tacgacaggc atgtatagca
    16441 aactgaatct ggaata
//
LOCUS       DAVMEF010000032        16157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMEF010000032 DAVMEF010000000
VERSION     DAVMEF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.032"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            11..1069
                     /locus_tag="VYQ30_000725"
     CDS             11..1069
                     /locus_tag="VYQ30_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1965879.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLDTAVSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            1220..1585
                     /locus_tag="VYQ30_000726"
     CDS             1220..1585
                     /locus_tag="VYQ30_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1965880.1"
                     /translation="MYRVIEMYGDFELWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            1661..2602
                     /locus_tag="VYQ30_000727"
     CDS             1661..2602
                     /locus_tag="VYQ30_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1965881.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            2550..3566
                     /locus_tag="VYQ30_000728"
     CDS             2550..3566
                     /locus_tag="VYQ30_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1965882.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRAGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            3568..3894
                     /gene="comGC"
                     /locus_tag="VYQ30_000729"
     CDS             3568..3894
                     /gene="comGC"
                     /locus_tag="VYQ30_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1965883.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQAKAYKEYHDKNGGA
                     NRKVND"
     gene            3887..4291
                     /locus_tag="VYQ30_000730"
     CDS             3887..4291
                     /locus_tag="VYQ30_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1965884.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            4296..4556
                     /locus_tag="VYQ30_000731"
     CDS             4296..4556
                     /locus_tag="VYQ30_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965885.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            4519..4980
                     /locus_tag="VYQ30_000732"
     CDS             4519..4980
                     /locus_tag="VYQ30_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1965886.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            4958..5368
                     /locus_tag="VYQ30_000733"
     CDS             4958..5368
                     /locus_tag="VYQ30_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1965887.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKSEEKPEKKENS"
     gene            5505..6092
                     /locus_tag="VYQ30_000734"
     CDS             5505..6092
                     /locus_tag="VYQ30_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1965888.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAAEKQIDLLSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            6153..7106
                     /locus_tag="VYQ30_000735"
     CDS             6153..7106
                     /locus_tag="VYQ30_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1965889.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            7157..8347
                     /locus_tag="VYQ30_000736"
     CDS             7157..8347
                     /locus_tag="VYQ30_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1965890.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            8496..8867
                     /gene="rnpA"
                     /locus_tag="VYQ30_000737"
     CDS             8496..8867
                     /gene="rnpA"
                     /locus_tag="VYQ30_000737"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1965891.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            8842..9666
                     /locus_tag="VYQ30_000738"
     CDS             8842..9666
                     /locus_tag="VYQ30_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1965892.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            9685..10671
                     /locus_tag="VYQ30_000739"
     CDS             9685..10671
                     /locus_tag="VYQ30_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1965893.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            10722..11345
                     /locus_tag="VYQ30_000740"
     CDS             10722..11345
                     /locus_tag="VYQ30_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1965894.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKVFQANHPEWQLQQTSQWMNQAQRLRPHFWVYLQREGKVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            11476..12933
                     /locus_tag="VYQ30_000741"
     CDS             11476..12933
                     /locus_tag="VYQ30_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1965895.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLLIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            12956..13237
                     /locus_tag="VYQ30_000742"
     CDS             12956..13237
                     /locus_tag="VYQ30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1965896.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            13314..13799
                     /locus_tag="VYQ30_000743"
     CDS             13314..13799
                     /locus_tag="VYQ30_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1965897.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            13816..14481
                     /locus_tag="VYQ30_000744"
     CDS             13816..14481
                     /locus_tag="VYQ30_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1965898.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            14485..15348
                     /locus_tag="VYQ30_000745"
     CDS             14485..15348
                     /locus_tag="VYQ30_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1965899.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            15350..16054
                     /locus_tag="VYQ30_000746"
     CDS             15350..16054
                     /locus_tag="VYQ30_000746"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1965900.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         4955 a         2843 c         3693 g         4666 t
ORIGIN      
        1 ggagtgcttt atgaaagcct atacttatgt taaaccagga cttgcttctt ttgttgatgt
       61 agacaaacca gttattcgca agccaacaga tgctattgtg cgtattgtaa aaaccactat
      121 ttgtggaaca gacctccata ttatcaaagg ggatgttcct acttgccaaa gtggtaccat
      181 tcttggccac gaagggattg ggattgttga agaagttggg gaaggagttt ccaacttcaa
      241 aaaaggtgac aaggtcttga tttcttgcgt ctgtgcctgt ggtaaatgct actactgtaa
      301 aaaaggaatt tatgcccact gtgaagacga agggggctgg attttcggtc acttgattga
      361 tggtatgcag gctgaatatc tacgtgtccc tcatgcagat aatactcttt accatactcc
      421 agaagacttg tcagatgaag ctttggttat gctgtcagac attctgccta ctggatatga
      481 aattggtgtc ttaaaaggga aagtagaacc tggttgcagc gtagccatta ttggttcagg
      541 tccagttgga ttggctgctc ttttaacagc ccaattctat tcaccagcta aattgattat
      601 ggtggacttg gatgataacc gtttggatac tgcagtatcc tttggtgcta ctcataaggt
      661 aaactcttca gaccctgaaa aagcgataaa agaaatttat gatttgacag atggtcgcgg
      721 tgtagatgtc gctattgaag ctgtagggat tcctgaaaca tttgatttct gtcaaaagat
      781 tatcggtgta gacggaacgg ttgccaactg tggtgtgcat ggtaaaccag ttgaattcga
      841 tttagataaa ctttggattc gcaacatcaa tgtaacaact ggtttggtat ctacaaatac
      901 gactccacaa ttgttgaaag cacttgaaag tcataagatt gaaccagaaa aattggtaac
      961 tcactatttc aaactcagtg aaattgaaaa agcctacgaa gtcttcagta aggcagcaga
     1021 ccaccatgcc attaaggtca ttatcgaaaa cgatatctca gaagcctaag tagtaaaaat
     1081 atttttgtac ataagcaaat agaaattcag tcatccatca gatggctgga ttttttatca
     1141 aaaaattaag aaatgagcat atttctttcc ttgtctggcg gaattggtta taatatacgg
     1201 tacaaaggaa tgaatgaata tgtatcgtgt tatagaaatg tacggagatt ttgaactgtg
     1261 gtggttctta gaaggttggg aagaagatat tgtagcaagt agaaaatttg accagtatta
     1321 tgatgctctc aaatactaca aaacttgctg gtttagattg gaacaagaat cccctcttta
     1381 taaaagtaga agcgacttga tgaccatttt ttgggacccg gaagaccaac gctggtgtga
     1441 tgaatgtgat gagtatttac aacaatacca ttctttggct cttttgcagg atgagcaggt
     1501 tatcccagac gaaaaactac gctcaggcta tgaaaaacaa accagtcagg aaaggaatcg
     1561 ttcttgccgt atgaaattaa aatagagaaa agtaactttt ttggagttgc tttttttatt
     1621 tttctaacgc tttgcgaata gtataggtga ggaggtaagt atggttcaag aaattgcaca
     1681 agaaatcatt cgttcggctc ggaaaaaagg ggcgcaagac atttattttg tccccaagtt
     1741 agatgcctat gagcttcata tgagggtagg agacgagcgc tgtaaaattg gtagctatga
     1801 ttttgaaaag tttgcagccg ttatcagtca ctttaagttt gtggcgggta tgaatgtggg
     1861 agaaaaaaga cgtagtcaac tgggttcctg tgattatgcc tatgaccaga agatagcgtc
     1921 tctacgttta tctactgtag gcgattatcg ggggcatgag agtttggtta tccgtttgtt
     1981 gcacgatgag gagcaggact tgcatttttg gtttcaggat attgaagaat taggcaagca
     2041 gtacaggcaa cggggactct atctttttgc tggtccggtt gggagtggta agacgacctt
     2101 gatgcatgaa ttgtccaagt cactctttaa aggacagcaa gttatgtcca tcgaagatcc
     2161 tgtcgaaatc aagcaggacg acatgcttca gttgcagttg aacgaagcaa tcggcctaac
     2221 ctatgaaaat ctaatcaaac tttccttgcg tcatcgacca gatctcttga ttatcggaga
     2281 aattcgtgac agcgagacgg cgcgtgcagt ggtcagagct agtttgacag gtgcgacagt
     2341 cttttcaacc attcacgcca agagtatccg aggtgtttat gagcgtctgc tggagttggg
     2401 tgtgagtgaa gaagaattgg cagttgttct gcaaggagtc tgctaccaga gattaatcgg
     2461 gggaggagga atcgttgact ttgcaagcag agattatcaa gaacaccaag cagccaagtg
     2521 gaatgagcaa attgaccagc ttcttaaaga tggacatatc acaagtcttc aggctgagac
     2581 ggaaaaaatt agctacagct aagcaaaaaa atatcatcac cctatttaac aatctctttt
     2641 ctagcggttt tcatctggtg gagaccatct cctttttaga taggagtgct ttgttggaca
     2701 agcagtgtgt gacccagatg cgtgcgggct tgtctcaggg gaaatcattc tcagaaatga
     2761 tggaaagttt gggatgttca agtgctattg tcactcagtt atccctagct gaagtccatg
     2821 gcaatctcca cctgagtttg ggaaagatag aagagtatct agacaatctg gccaaggtca
     2881 agaaaaaatt aattgaagta gcgacctatc ctttgatttt gctgtttttt cttctcttaa
     2941 ttatgctggg gctacggaat tacctgctcc cacaactgga tagtagcaat attgccaccc
     3001 gaattatcgg taatctgcca caaatttttc taggcatggt agggcttgtt tccgtgcttg
     3061 cccttttagc actaactttt tataaaagaa gttctaagat gagtgtcttt tctatcttag
     3121 cacgccttcc ctttattgga atctttgtgc agacctactt gacagcctat tatgcacgtg
     3181 aatgggggaa tatgatttca cagggaatgg agctgacgca gatttttcaa atgatgcagg
     3241 aacaaggttc ccagctcttt aaagaaatcg gtcaagatct ggctcaaacc ctgaaaaatg
     3301 gccgtgaatt ttctcagacg ataggaacct atcctttctt taggaaggaa ttgagtctca
     3361 tcatagagta tggggaagtt aagtccaagc tgggtagtga gttggaaatc tatgctgaaa
     3421 aaacttggga agcctttttt acccgagtca accgcaccat gaatttggtg cagccactgg
     3481 tttttatctt tgtggcactg attatcgttt tactttatgc ggcaatgctc atgcccatgt
     3541 atcaaaatat ggaggtaaat ttttaaaatg aaaaaaatga tgacattctt gaaaaaagct
     3601 aaggttaaag cttttacatt ggtggaaatg ttggtggtct tgctgattat cagcgtgctt
     3661 ttcttgctct ttgtacctaa tctgaccaag caaaaagaag cagtcaatga caaaggaaaa
     3721 gcagctgttg ttaaggtggt ggaaagccag gcagaacttt atagcttaga aaagaatgaa
     3781 gatgctagcc taagaaagtt acaagcagat ggacgcatca cggaagaaca ggctaaagct
     3841 tataaagaat accatgataa aaatggagga gcaaatcgta aagtcaatga ttaaggcctt
     3901 taccatgctg gaaagtctct tggttttggg tcttgtgagt atccttgcct tgggcttgtc
     3961 cggctctgtt cagtccactt ttgcggcggt agaggaacag attttcttta tggagtttga
     4021 agaactctat cgggaaaccc aaaaacgcag tgtagccagt cagcaaaaga ctaatctaaa
     4081 tttagatggg cagacgctta gcaatggcag tcaaaagttg acagttccta aaggaattca
     4141 ggcaccatca ggccaaagta ttacatttga ccgagctggg ggcaattcgt ccctggctaa
     4201 ggttgaattt cagaccagta aaggagcgat tcgctatcaa ttatatctag gaaatggaaa
     4261 aattaaacgc attaaggaaa caaaaaatta gggcagtgat tttactggaa gcagtagtcg
     4321 ctctagctat ctttgccagc attgcgaccc tccttttggg acaaattcaa aaaaataggc
     4381 aagaggaagc aaaaatcttg caaaaggaag aagtcttgag ggtagctaag atggccctgc
     4441 agacggggca aaatcaggta agcatcaacg gagttgagat tcaggtattt tctagtgaaa
     4501 aaggattgga ggtctaccat ggttcagaac agttgttggc aatcaaagag ccataaggtc
     4561 aaggctttta ccttgttaga atccctactt gccctcattg tcatcagtgg gggattactc
     4621 ctttttcaaa ctatgagtca gctcctcatt tcagaagttc gctaccagca acaaagcgag
     4681 caaaaggagt ggctcttgtt tgtggaccaa cttgaggcag aattagaccg ttcgcagttc
     4741 gaaaaagtag aaggcaatcg cctatacatg aagcaagatg gcaaggacat cgccatcggt
     4801 aagtcaaagt cagacgattt tcgtaaaacc gatgctagtg gacgggggta tcagcctatg
     4861 gtttatggcc tcaaatcagc tcagattaca gaggacaatc aactggttca ttttcgtttc
     4921 cagtttcaaa aaggcttaga aagggagttc atctatcgtg tggaaaaaga aaaaagttaa
     4981 ggcaggtgtt ctcctctacg cagtcaccat agcagccatc tttagtcttt tgttgcaatt
     5041 ttacttgaac cgacaagtcg cccactatca agactatgct ttgaataaag aaaaattggt
     5101 tgcttttgca atggctaaac gaaccaagga taaagccgag caagaaagtg gggaacaggt
     5161 ctttaatcta ggtcaggtaa gctatcaaaa caagaaaaca agcttggtga cgactgttcg
     5221 tacgtctaag agccaatatg agtttctgtt tccttcagtc aaaatcaaag aagagaaaag
     5281 agataaaaag gaagaggtag cgactgattc aagcgaaaaa gcggagaaaa aatcagaaga
     5341 gaagcctgaa aagaaagaga attcatagtc aattcaacta taatgcgttg aatccagaat
     5401 agtccactgt agtttctaga aaattgctgg aaatggatgt taagctccaa ttcatttgtt
     5461 tatatcttat ttcagtttac tatactttgt gctaaattaa agatatgaaa catgatttta
     5521 accacaaagc agaaactttc gattccccta aaaatatctt cctcgcaaac ttggtatgtc
     5581 aagcagccga gaaacagatt gatcttctat cagacaaaga aattttagat ttcggtggtg
     5641 gcacgggtct attagcctta cccctagcca agcaggctaa gtcagtcact cttgtagaca
     5701 tttctgagaa aatgttggag caagctcgtt tgaaagtgga gcagcaagca atcaagaata
     5761 tccagttttt ggagcaagat ttaccgaaaa atcccttgga gaaagagttt gattgccttg
     5821 ctgttagtcg ggttcttcat catatgcctg atttggatgc ggctctctca ctgtttcatc
     5881 aacatttgaa ggaagatggg aaactcatca ttgctgattt taccaagaca gaagctaatc
     5941 atcatggatt tgatttagct gaactggaaa acaagctaat tgagcatggt ttttcatctg
     6001 tgcatagtca gattctctat agcgctgaag acctgtttca aggaaatcac tcagaattct
     6061 ttttaatagt agcccaaaaa tcactcgcct agtcagggag tgatttttct ataaggatgg
     6121 aaaaaagaag ggaaatttgg taagatagga atatggattt tgaaaaaatt gaacaagctt
     6181 atacctattt actagagaat gtccaagtca tccaaagtga tttggcgacc aacttttatg
     6241 acgccttggt ggagcaaaat agcatctatc tggatggtga aactgagcta aaccaggtca
     6301 aagacaacaa tcaaaccctt aagcgtttag cactacgcaa agaagaatgg ctcaagacct
     6361 accagtttct cttgatgaag gctgggcaaa cagaaccctt gcaggccaat caccagttta
     6421 caccggatgc tattgctttg cttttggtgt ttattgtgga agagttgttt aaagaggagg
     6481 aaattactat cctcgaaatg ggttctggga tgggaattct aggcgctatt ttcttgacct
     6541 cgcttgataa aaaggtggat tacttgggaa tggaagtgga tgatttgctg attgatctgg
     6601 cagctagcat ggcagatgta attggtttgc aggctggctt tgtccaagga gatgccgttc
     6661 gcccacaaat gctcaaagaa agcgatgtgg tcatcagtga cttgcctgtc ggctattatc
     6721 ctgatgatgc cgttgcgtcg cgccatcaag ttgcttctag ccaagaacat acttacgccc
     6781 atcacttgct catggaacaa gggcttaagt acctcaagtc agacggatac gctatttttc
     6841 tagctccgag tgatttgttg accagtcctc aaagtgattt gttaaaagaa tggctgaaag
     6901 aagaggcgag tctggttgct atgattagtc tgcctgaaaa tctctttgct aatgccaaac
     6961 aatctaagac tatttttatc ttacagaaga aaaatgaaat agcagtagag ccttttgttt
     7021 atccacttgc tagcttgcaa gatgcaagtg ttttaatgaa atttaaagaa aattttcaaa
     7081 aatggactca aggtactgaa atataaaata gattttgtta taatagttga aaacgcttaa
     7141 aaaggggtat catgttatga caaaaacaat tgcaatcaat gcaggaagtt caagtttgaa
     7201 atggcaatta tacttaatgc cagaagaaaa agtattggcg aaaggtttga ttgaacgtat
     7261 cggtttgaaa gattcaattt caactgtaaa atttgacggc cgttctgaac aacaaatttt
     7321 ggatattgaa aatcatacac aagccgttaa aattttattg gatgacttga ttcgtttcga
     7381 tattatcaag gcttatgacg agattacagg tgttggacat cgtgttgttg ctggtggaga
     7441 atatttcaaa gaatcaacag ttgttgaggg agatgtttta gaaaaagttg aagagttgag
     7501 tttgttggct cctctacaca acccggccaa tgcagcaggt gttcgtgcct tcaaggaatt
     7561 gttgccagac attaccagtg tagttgtttt tgatacttcc ttccacacaa gtatgccaga
     7621 gaaagcttat cgctaccctc taccaacaaa atattacaca gaaaacaagg ttcgtaaata
     7681 cggtgctcat ggtacaagtc accagtttgt agcaggagaa gctgcaaaac tcttgggacg
     7741 tccattagaa gacttgaagt taattacctg tcatattggt aacggaggct caattacagc
     7801 tgtgaaagcc ggcaaatctg tagacacttc tatggggttc actcctcttg gtggtattat
     7861 gatgggaacg cgtacagggg atattgatcc agctatcatt ccttatttaa tgcaatatac
     7921 agaggatttt aacacaccag aagatatcag tcgtgttctt aaccgtgaat caggtctttt
     7981 gggagtttct gctaattcta gcgatatgcg cgatatagaa gcagctgtag cagaagggaa
     8041 tcacgaggct agcttggctt atgaaatgta tgttgaccgt atccaaaaac atatcggtca
     8101 gtaccttgca gtgctaaatg gagcagatgc cattgttttc acagcaggtg tcggtgaaaa
     8161 tgcagagagt ttccgtcgtg atgtaatctc agggatttcg tggtttggtt gtgatgttga
     8221 tgatgaaaag aatgtctttg gcgttacagg agacatctca acagaggcag ctaaaatccg
     8281 tgtcttggtt attccaacag atgaagaatt agtcattgcc cgtgacgttg aacgcttgaa
     8341 aaaataagtg aaactaaaaa aatattcaat acaaggagtt gggaaagtta tttttccagc
     8401 ttctttttct gatgaaattg tccaaaacct tgctatgatt ggcttttttg aaaaatatgg
     8461 tataatagta gtaatttaat agatggagtt gagttttgaa gaaaaacttt cgtgtaaaaa
     8521 gagagaaaga ttttaaggcg attttcaagg aggggacaag ttttgctaat cgcaaatttg
     8581 tggtctacca attagaaaac cagaaaaacc attttcgagt aggtctatca gttagcaaaa
     8641 aactggggaa tgccgtcact agaaatcaaa ttaagcgacg gattcggcat attatccaga
     8701 atgcaaaagg gagtctggta gaagatgtcg actttgttgt cattgctcga aaaggagtcg
     8761 aaaccttggg atacgcagag atggagaaaa atctactcca tgtattaaaa ttatcaaaga
     8821 tttaccggga aggaaatggg agtgaaaaag aaactaaagt tgactagttt gctaggactg
     8881 tctctgttaa tcatgacagc ctgtgcgact aatggggtaa ctagcgatat tacagccgaa
     8941 tcggctgatt tttggagtaa attggtttac ttctttgcgg aaatcattcg ctttttatcg
     9001 tttgatatta gtatcggagt ggggattatt ctctttacgg tcttgattcg tacagtcctc
     9061 ttgccagtct ttcaggtgca aatggtggct tctaggaaaa tgcaggaagc tcagccacgc
     9121 attaaggcgc ttcgagaaca atatccaggt cgagatatgg aaagcagaac caaactagag
     9181 caggaaatgc gtaaagtatt taaagaaatg ggtgtcagac agtcagactc tctttggccg
     9241 attttgattc agatgccggt tattttggcc ctgttccaag ccctatcaag agttgacttt
     9301 ttaaagacag gtcatttctt atggattaac cttggtagtg tggatacaac ccttgttctt
     9361 ccgattttag cagcagtatt caccttttta agtacttggt tgtccaacaa agctttgtct
     9421 gagcgaaatg gcgctacgac tgcgatgatg tatgggattc cagtcttgat ttttatcttt
     9481 gcagtttatg cgccaggtgg agtcgcccta tactggacag tgtctaatgc ttatcaagtc
     9541 ttgcaaacct atttcttgaa taatccattc aagattatcg cagagcgcga ggccgtagta
     9601 caggcacaaa aagatttgga aaatagaaaa agaaaagcca agaaaaaggc tcagaaaacg
     9661 aaataaataa ggaggaatct ggtaatggta gtatttacag gttcaactgt tgaagaagca
     9721 atccagaaag gattgaaaga attagatatt ccaagaatga aggctcatat caaagtcatt
     9781 tctagggaga aaaaaggctt tcttggtcta tttggtaaaa aaccagccca agtggatatt
     9841 gaagcgatta gtgaaacgac tgttgttaaa gcaaatcaac aggtagtaaa aggcgttccg
     9901 aaaaaaatca atgatttgaa cgagcctgtg aagacggtta gtgaagaaac cgttgacctt
     9961 ggtcatgtgg ttgatgctat taaaaaaata gaggaagaag gtcaaggtat ttctgatgaa
    10021 gtcaaggctg aaatcttaaa acatgaaaga catgccagca ctatcttaga agaaactggt
    10081 cacattgaga ttttaaatga acttcaaatc gaggaagcga tgagggaaga agcaggcgct
    10141 gatgaccttg aaactgagca agatcaaact gaaaatcaag acttgaaaga gatgggcttg
    10201 aaggtcgagc aaagttatga tattgcccag gtggctacgg atgtgactgc ttatgttcaa
    10261 acgattattg atgacatgga tgttgaggct acactttcaa atgattataa ccgtcgtagc
    10321 atcaatttgc aaattgatac taacgaacca ggtcgtatta tcggctacca tggtaaagtc
    10381 ttgaaggcct tgcaactgtt ggctcaaaat tatctttaca actgctattc aagaaccttc
    10441 tatatcacta ttaatgtcaa tgattatgtt gaacaccgtg cagaagtctt gcagacctat
    10501 gcgcaaaaat tggcgattcg tgttttagaa gaaggacgta gtcatcaaac agatccaatg
    10561 tcgaatagtg aacgcaagat tatccaccgt attatttcac gtatggatgg cgtgaccagt
    10621 tactctgaag gtgatgagcc aaatcgctat gtcgttgtag atacagaata agtaaaatca
    10681 gggttgacct gattttttgc tagctagagg aggttaaact gatgttgaat aagataagag
    10741 actatctaga cttttctggt ttgcagtacc gtagtcctga taaagcggga gcagagcgag
    10801 agaagatgct ggcattccgc cacaaaggtc aagaggcacg aaaggctttt acagaattgg
    10861 ctaaagtctt tcaggcaaat catccagaat ggcaactcca acagactagc cagtggatga
    10921 atcaggccca gcgtttgcga ccacattttt gggtttatct acaacgtgag gggaaagtga
    10981 cagaacctat gatagccttg cgtttgtatg gaaatccttc tgattttggt atttccttag
    11041 aggtcagttt catcgaacgt aagaaggatg agcaaacact gggcaagcag gccaaagttt
    11101 tagacattcc aaccgttaaa gggatttatt atctaaccta ctctaatggt caaagtcaac
    11161 ggtgggaggc gaatgaagaa aagcgtcgta ctttacgcga gaaggtgaga agtcaagaag
    11221 ttcgaaaagt tttagtgaag gtagatgttc ctatgacaga aaattcgtct gaagaagaaa
    11281 tcgtagaagg cttattgaag tcttattcta aaattcttcc ctattatcta gctacgagaa
    11341 aataagataa tttgtaaaac atcataaatc atacagtcca agagtgaaca gtccgctgtg
    11401 taattcttgg tctttttgtt tgcgctttcg cattatataa taaacttaca aaaacaattc
    11461 aaaaggagaa caattatgga agtcgtttca agtgttctaa attggttttc tagcaatatt
    11521 ttgcagaatc ccgcattttt cgtaggttta ttggtgttga taggatatgc acttttgaaa
    11581 aaacctgccc atgacgtttt ttcagggttt gttaaagcaa cagtagggta tatgttgctt
    11641 aacgtgggtg ctggtggttt ggttacaacc tttcgtccaa tcttagcagc tcttaactac
    11701 aaattccaaa ttggtgcagc ggttatcgac ccttactttg gacttgctgc agcaaacaac
    11761 aaaattgcag cagagtttcc agattttgtt ggaactgcaa ctacagctct attgattggt
    11821 ttcggaataa atatcttgct cgtagctctt cgaaagatta cgaaggtaag aaccctcttt
    11881 attactggtc acatcatggt acaacaagct gcaacagtat ctcttatggt tctattctta
    11941 gtaccacaat tgcgcaatgc ttacggtaca gcagcgattg gtatcatctg tggactttac
    12001 tgggcagtta gttcaaatat gactgttgag gcaactcaac gcttgactgg tggtggcgga
    12061 tttgcgattg gtcaccaaca gcaatttgca atctggtttg tagataaagt agcaggacgc
    12121 tttggtaaga aagaagaaag tttagacaat cttaaattac ctaagttcct cttaatcttc
    12181 cacgatacag ttgttgcatc tgctaccttg atgctcgtat tcttcggggc cattctttta
    12241 atcttgggtc cagacattat gtctaataaa gaagtcatca cttcaggaac tctattcaat
    12301 cctgctaaac aagatttctt tatgtacatt atccaaacag cctttacctt ctcagtttac
    12361 ttgttcgttt tgatgcaagg tgtccgaatg ttcgtatctg agttgacaaa cgccttccaa
    12421 ggtatttcaa acaaattgtt gccaggttca ttcccagcgg ttgacgttgc agcttcttat
    12481 ggatttggtt ctccaaatgc tgtcttgtca ggatttacct ttggtttgat tggtcaattg
    12541 attacaattg ttttgctcat cgtctttaaa aatccgattc ttattattac aggatttgta
    12601 ccagtgttct ttgacaatgc agccattgcg gtctacgctg ataaacgcgg cggatggaaa
    12661 gcggctgtta tcctttcctt tatatcaggt gtccttcaag ttgctctagg agctctttgt
    12721 gtggcccttc tcgatttggc atcttatggt ggctaccatg gaaatatcga ctttgaattc
    12781 ccatggcttg gatttggata tatcttcaaa taccttggta ttgttggtta tgtacttgtg
    12841 tgtctcttct tgcttgttat tcctcaactt caatttgcca aagcaaaaga taaagagaaa
    12901 tattacaacg gtgaagttca agaagaagct tagtatctag aaaaggagaa ataaaatggt
    12961 taaagtatta gcagcgtgcg gaaatggaat gggttcatca atggttatca agatgaaggt
    13021 tgaaaatgct ctccgtaagc ttaatcaaac agattttaca gtcaattcat gcagtgtcgg
    13081 tgaagctaaa ggtttagcag taggatatga catcgtaatc gcttctcttc atttgattca
    13141 agaattggaa gggcgaacta atgggaagtt aattgggctt gataacttga tggatgataa
    13201 agaaatcacc gaaaaactca gtcaagcact acagtaaaag gttggagggg gctggacaga
    13261 aactgagagt tatcgtttct gtccttctcc ctctttaaat aaaggaggca gatatgaatt
    13321 taaaacaagc tttaattgac aatgactcga tccgactagg tttagaggct aacaattgga
    13381 aagaagcagt caaggtagca gtagatccct taattgaaag tggggcaatt ttgccagagt
    13441 attacgatgc tatcattgaa tcgactgaag agtatgggcc ttactatatc ttgatgccag
    13501 gtatggctat gccccacgct agacctgaag ctggtgtgca aagtgatgcc ttttcattga
    13561 ttaccttaca aaatcctgtt gtattttcag atgggaaaga ggtatctgtt ttgttggcac
    13621 tagcagcaac aagctcaaaa attcacacaa gtgtagccat tccacaaatt attgccctgt
    13681 ttgaattaga agattctatt gcacgtttac aggcttgcca gactaaagaa gatgtcttgg
    13741 ctatgattga agaatctaag gatagccctt atctcgaagg attggacttg gaaagttaga
    13801 aagaggaata aagaaatgac aaaaagaata cctaatttac aagttgcatt agaccattca
    13861 gacttgcaag gagcgattaa agcagctgtt tctgttggtc aggaagtaga tattatcgaa
    13921 gctggaactg tttgcttgct tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt
    13981 ttcccagata agattattgt ggcagacaca aaatgtgctg atgctggtgg aacagttgct
    14041 aaaaataatg cggttcgtgg agcagactgg atgacttgta tctgttgtgc aaccatccct
    14101 actatggaag cagctctaaa ggctatcaag actgaacgag gagaacgagg cgaaatccag
    14161 atcgagcttt atggcgattg gacttttgaa caagctcagc tttggctaga tgcaggtatc
    14221 tcacaagcta tttatcacca atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa
    14281 aaagacctta ataaggttaa aaaactcatt gacatgggct tccgtgtatc tgtaacaggt
    14341 ggtctagatg tagatactct caaactcttt gaaggtgttg atgtctttac ctttatcgca
    14401 ggtcgtggaa ttacagaggc tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa
    14461 atcaaacgaa tttgggggta aatcatggta cgtccaattg gaatttatga aaaggcaacc
    14521 ccaacacact gtacttggct agaacgttta aattttgcca aggagttagg ctttgatttt
    14581 gtcgagatgt ctattgacga acgtgacgag cgtttagcaa gacttgactg gagtaaggaa
    14641 gaacgcttgg aagttgtcaa agcaatctat gaaactggtg ttcgtattcc ttctatctgt
    14701 ttttcaggcc atcgtcgcta cccattgggt tcaaaagatc cagttctaga ggaaaaatct
    14761 ctagaactca tgaaaaaatg tatcgaatta gctcaagact tgggagttcg tacgattcaa
    14821 ttagctggtt acgatgttta ctatgaggaa aagtcacccc agacacgcca acgttttatc
    14881 aaaaatttga gaaaagcctg tgactgggct gaagaagctc aggtggtact tgctattgaa
    14941 attatggatg atcctttcat caatagcatc gaaaaatatt tggctataga aaaagagatt
    15001 gactctccct tcctctttgt atatccagat attggtaatg tgtctgcatg gcataatgat
    15061 atctatagtg agttttatct tggtcatcat gccatcgcag ctctccatct caaggatact
    15121 tatgcagtga cagaaagttc aaagggccag ttccgagatg tacctttcgg gcaaggttgt
    15181 gtcaaatggg aagaagcttt cgatatttta aaggaaacca attataatgg acctttccta
    15241 atcgaaatgt ggtctgaaaa ttgtgaaaca gtagaagaaa cacgcgcagc cgttcaagag
    15301 gcgcaagctt ttctctatcc actcattaag aaagcaggtt tgatgtaaga tgaatcaagt
    15361 aatcaatgct atgcgtaaac gagtctgtga tgccaatcaa tcattgccaa aacatggact
    15421 tgtcaaattt acctggggga atgtatctga agttaatcgc gaactcggtg tcattgttat
    15481 caaaccatca ggcgtggatt atgacgaatt gacacctgaa aacatggtag tgactgatct
    15541 agatggtaag atcctagaag gggatttaag accatcttcc gacctcccaa ctcatgtgca
    15601 attatataag gcttggtcag aaattggtag tgtggttcac acccattcga cagaagctgt
    15661 tggttgggct caggcaggtc gtgatattcc tttctacgga acaacccatg cagattattt
    15721 ctacggttca atcccttgcg cccgtagttt gaccaaggac gaagtagaag tggcctatga
    15781 aaaagatact ggcctggtta tcgtagaaga gtttgaacat cgcggactta acccggttga
    15841 agtaccagga attgttgtac gcaatcacgg tccattcacc tggggtaaaa atccagagaa
    15901 tgctgtttat cactctgtcg tactagagga agtatcaaag atgaatcgct ttacagaaca
    15961 aatcaatcca agagttggac ctgctcccca gtacatacta gaaaaacact accaacgtaa
    16021 acatggacca aatgcttatt atggtcaaaa gtaagaacga tgaaggagga gaaaaagata
    16081 aatttagctc ctctttttac atttgatttt tattgagagt aaagttggag ttgaagtaat
    16141 tttgaaagat tttttag
//
LOCUS       DAVMEF010000033        15575 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMEF010000033 DAVMEF010000000
VERSION     DAVMEF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.033"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(156..1718)
                     /gene="guaA"
                     /locus_tag="VYQ30_000747"
     CDS             complement(156..1718)
                     /gene="guaA"
                     /locus_tag="VYQ30_000747"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1965901.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1860..2558
                     /locus_tag="VYQ30_000748"
     CDS             1860..2558
                     /locus_tag="VYQ30_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1965902.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2601..3497)
                     /locus_tag="VYQ30_000749"
     CDS             complement(2601..3497)
                     /locus_tag="VYQ30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1965903.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATTKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIILAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3506..4441)
                     /locus_tag="VYQ30_000750"
     CDS             complement(3506..4441)
                     /locus_tag="VYQ30_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1965904.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4438..5163)
                     /locus_tag="VYQ30_000751"
     CDS             complement(4438..5163)
                     /locus_tag="VYQ30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1965905.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5247..5882)
                     /locus_tag="VYQ30_000752"
     CDS             complement(5247..5882)
                     /locus_tag="VYQ30_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1965906.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5884..6711)
                     /locus_tag="VYQ30_000753"
     CDS             complement(5884..6711)
                     /locus_tag="VYQ30_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1965907.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6733..7659)
                     /locus_tag="VYQ30_000754"
                     /pseudo
     CDS             complement(6733..7659)
                     /locus_tag="VYQ30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8310..8921
                     /locus_tag="VYQ30_000755"
     CDS             8310..8921
                     /locus_tag="VYQ30_000755"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1965908.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            8966..9694
                     /gene="rlmB"
                     /locus_tag="VYQ30_000756"
     CDS             8966..9694
                     /gene="rlmB"
                     /locus_tag="VYQ30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1965909.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9733..10644)
                     /gene="trxB"
                     /locus_tag="VYQ30_000757"
     CDS             complement(9733..10644)
                     /gene="trxB"
                     /locus_tag="VYQ30_000757"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1965910.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQERAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWILTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10710..10934)
                     /locus_tag="VYQ30_000758"
     CDS             complement(10710..10934)
                     /locus_tag="VYQ30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1965911.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11012..11755)
                     /locus_tag="VYQ30_000759"
     CDS             complement(11012..11755)
                     /locus_tag="VYQ30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1965912.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11755..12555)
                     /locus_tag="VYQ30_000760"
     CDS             complement(11755..12555)
                     /locus_tag="VYQ30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1965913.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12713..13069)
                     /locus_tag="VYQ30_000761"
     CDS             complement(12713..13069)
                     /locus_tag="VYQ30_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1965914.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13063..13593)
                     /locus_tag="VYQ30_000762"
     CDS             complement(13063..13593)
                     /locus_tag="VYQ30_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1965915.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13593..14087)
                     /locus_tag="VYQ30_000763"
     CDS             complement(13593..14087)
                     /locus_tag="VYQ30_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965916.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14222..14668
                     /locus_tag="VYQ30_000764"
     CDS             14222..14668
                     /locus_tag="VYQ30_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1965917.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14665..15312
                     /locus_tag="VYQ30_000765"
     CDS             14665..15312
                     /locus_tag="VYQ30_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1965918.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
BASE COUNT         4673 a         3547 c         2773 g         4582 t
ORIGIN      
        1 cttgaaaact acggtctaat tttttgtcca aatgtaaacc acttggaact attattcaat
       61 ttattctttt tagtaaatgt tcccgtttta atcccagtta cagtttttaa tcctctgtaa
      121 ccgttgattt tacaaagctt ttaatttttt tgcgattact cccactcaac tgttgcaggt
      181 ggtttacttg taatatcgta gacgatacgg ttaacatgat ccacttcatt tacgatacgt
      241 actgagattt tttgaagtac ttcccatgga attttggcaa aatcagcagt cataccatcg
      301 atagaagtga tagcacggat tgcaatcgtg tagtcatacg tacgaccgtc acccataaca
      361 ccgactgaac gaacgcctgt gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca
      421 gctttagcga tttcttcacg aagaatagcg tctgattcac gaacggtttc aagtttctct
      481 tcagtgattt cacccatgac acggatagca agtcctggtc ctgggaatgg ttggcgccat
      541 acgatatggt ctggcatacc aagctctgta ccaagagcac gaacttcatc cttgtaaaga
      601 gtattgagtg gttcaatcaa ttcaaactgc atatcttctg gaagaccacc cacgttgtgg
      661 tgtgacttga tagtttgagc tgtatccgta ccagactcga taacatctgt atataaagta
      721 ccttgagcaa ggaatttcac atctttgagc ttgcttgctt cgtcatcgaa tacatagaca
      781 aactcgttac cgatgatttt acgtttttgt tcagggtcag aaacgccagc aagtttgtca
      841 aggaaacgtt tagcagcgtc tgctttgacg atattcaaac caaacttacc accgagcatg
      901 tccataactt gatcagcttc gcctttacga agaagaccgt ggtctacgaa gatacagatc
      961 aattgatcgc caatcgcttt ttggagaaga accccaacga cagatgagtc aacaccacct
     1021 gatagaccaa gaaggacacg tttatcaccg acggtttcac gaattttttt gatctgcatg
     1081 tcaatgaaat tatccattga ccagtcacct ttagccttac aaatgttaag ggcaaagtta
     1141 cgaaggatat catttccgta tacagaatga cgaacttctg ggtggaattg gataccgtaa
     1201 atgtgtttat ctgggttttc gatggctgcg tatgggcagt cagctgatgt acctgtacga
     1261 acaaagtcag caggaatctc agtaaccgca tcaccatggc tcatcaaaac agtctgttca
     1321 tcaggtgttg attcaaaaag cgctgatggt gtgtgagtta gggttgattg accgtattca
     1381 cgatttccag catcacctgc aggaacaact tttcctccaa gtttatgggt caataactgc
     1441 ataccataac agattcccaa aattggaatt ccgagttcga agatttctgg gtcaatatca
     1501 aatgaaccat cttcatatac agaatttgga ccacctgata gaataattcc tacaggattg
     1561 acttcacgaa cttcagcagc tgaaatttta tggcttttta gttctgaaaa aacaccaatc
     1621 tcacggatac ggcgtgaaat cagctggttg tactggctac catagtccaa tacgatgatt
     1681 ttttctacat cttgcaaatc agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa
     1741 tatcttttcc caatattcta tcacaaataa gggcgaatta ccaactaaac aaggcaaagt
     1801 ttgacttttt ctagtttatt ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata
     1861 tgctacctgc ttatatgaaa atccatgatc agataaaaaa ggatattgac gagcaccgtt
     1921 gggctattgg tgagaggctt cccagtgaaa gagatttagc tgagcagttt gcggtcagtc
     1981 gcatgaccct ccgccaagcc gtatctctat tagtcgaaga aggcgtctta gagcgccgtg
     2041 taggaagcgg cacctttgtt tccagtactc gagtacaaga aaagatgcga gggacaacca
     2101 gttttactga aattgtcaaa tcccaaggta aagttccctc tagccagctc atttcctaca
     2161 aaaaaaccat tcccaatgag caagaagttg ccaagctagg aatttttcca acggacaata
     2221 ttatccgaat ggagcgggtc cgctatgccg accaagttcc cctagtttat gaagttgctt
     2281 ctattcctga aaaattcatt aaggacttta aaaaagaaga aatcaccagt catttcttcc
     2341 aaaccttgca aaaacatggc tatcgtatcg gtaaatccca acagaccatc tatgctcgcc
     2401 ttgctaaaga aaagattgcc cactatttgg aagttgaaaa aggacatgct attcttggat
     2461 tgacccagat ttcctaccta gaagatggta ctgcttttga atacgtaaaa agtcaatatg
     2521 taggcgaacg ctttgaattt tatcttgaaa ataattagat taccacgcaa aaactctctg
     2581 tcacggacaa agagtctttt ttatttttct aagaataaat ctttcaaaga aatcaaagtt
     2641 acagcaaaca atatcattgc cataccaaga aaatcaatgg gataaaattg ttcattcatt
     2701 attaagaagg caaagaaaat cgccgatatt ggctcaattg aagccaacaa gcttgacttg
     2761 accggtccta tcagactggc tcctttaagg aaagctgtat aggcaaagac agtcccgata
     2821 aggataatgc ctgcaaacgc aaggagaaaa tcaagactag tcgggatatc ggcctgtaga
     2881 acccctgtaa aaggaagggc gaccaaacct gctatgacca ttcccacacc aatgaccaag
     2941 ctgctccccc actttttaat caaggctatg ggtaaaatga tatacagagc ataagtcaag
     3001 gcagaaaaga gaccccagaa caggccagca ggtgtcatgg ataactggtc caactgccca
     3061 tgtgttgcga tcaggaaggt tcctccgatg gccaatatga tggaaactat ctctcccagt
     3121 gtcggtgcca ccctatcctt gatacagcta taaattaaaa ttccgacagg acaaacatac
     3181 tgaagcaccg tcgctgttcc cgcattggtc tcctgaatag cagacagata ggcgaattgg
     3241 ttgagaaaaa gaccaatcag agcaaaaata agaagagaca gcaaactctt tctatccttt
     3301 aaaaagacca gtattttatc ctttgtagta gcataagcca agagcatgag aattccacca
     3361 gcgattaaaa gacgcaagtt ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat
     3421 tggccactcg ttcctgacaa cccccaagca ataccagcca caactgttag taaagtccct
     3481 tttaaactat ttgacatgtc tctccttact cttctttgcg aatcaagttc atcacttgat
     3541 tgcggtctaa tatccattga gctctctcgt ccttttcata ggtacgattc gataggaaaa
     3601 tagtggcttc ttgcttctga cgattccaca tgataaaggt acctgtatag cccgtatggt
     3661 ctagccaatc tccttccaaa ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa
     3721 aattttgatt taagtctctt gcaaaatcat ctgctaaata gtgttctaaa aagatttgta
     3781 aatcctttat agtcgaaaat aaaccagcac tcccagcatg tctacccagg agacgagcct
     3841 tgggatcatg cactatgcct gcctctacac ctctaactgt tggaacagca agctcaactg
     3901 gcccaaactt agtttccgtc attccccaag gtttccagac ttgatccttt aaaatcacat
     3961 ccaaatcttg attaaaaatt ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg
     4021 aataaagaaa ggctggctga cttcgtctgt tgagatgaaa catcgcttcc tttaattcag
     4081 gggctgttaa aagatcacga ttaggaataa aaggatcaag gtctgttgca tgagtcaaga
     4141 gctggcgaat agtgatgtct ggataatcac tctcaggtaa aaaatctatt accagtctat
     4201 caatatctaa ttgacctatt tcccacaaga aggtacaaac tgtgccaacc ccaacaacct
     4261 tgctgacact agctaggtca taaactagtc ctgcctcagt ctgcaagcca tgctctgggg
     4321 cactctggcc taaatagaac tctgtccatt gattgtcctt aaaatacgca aaagaggctc
     4381 cgggataaat tcctgcctcg atttgttctt ctattttttt aataatcttg gtccacttca
     4441 tacttcttca aaccacaatt caacattatt tctatcttta ccaaggaaga atttttcaga
     4501 cttaggaata aaatattcag tagactcaaa cttctggcga agacttgcta tgtctaattc
     4561 attgaccaag aacttgagca tagataagtc ccaggtaacc gtattgtcca cagtcaaatc
     4621 ctgcccctga gctggaataa aatcaaggga tgccccaatt tcagatgatt ccaagaaact
     4681 ttcgatatca gttgggagat gtaattccat agaaatctca aatttactta aagaaattga
     4741 tgccaaatct gtttgaaatt caggcttttc tcctacttct actagacttg ctatgtcatc
     4801 ttccgcatga atcaaaatca aatcatcttc tggtgagaaa atttcaaaag cgtagccatt
     4861 ttgaccttta tataatcgat gaatcgaatc tgttttagat aagattcctt caatttctaa
     4921 gggattttcc accttgacaa tcaatctagc tagttttttt cttccctcta ccttacgagt
     4981 acgcatactg ggagcttctt ctaaaaccag cttttcaaga cccgtttggt cacctagtga
     5041 cagaaaggcc gattcttcta acaaggcctt cattccaagg gtttcaatat aaaatgtttc
     5101 atttaatttt ctattattag cttttaaagt aggaataatc cgtacaatct gatttacatt
     5161 cataaattcc tccgtcactt tttattttaa aggattttag aattttttac aagatattat
     5221 gctcaaattt aacattattt agctatttag taaagatagt ctttcaagga ttttgacaaa
     5281 gcctgataac cagcaatact gaggtgcagt ccatcagttg tatattcttt tttgagttgg
     5341 cctgcttggt ctgtcaaaca atcaaatact ggcacaaatt ccacctgcat ataggcagat
     5401 gcaagctctt gataggcttg attccagttc tgaatttttt cattcgagcg gatatagact
     5461 gcctgctggt actcctctct ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc
     5521 aatggataat cgcgagcaac ggattgaatg atagcttcga gattattgag agcctcattc
     5581 acaggaacat cctttccgat atcatttgtc ccaatcagaa gaaaaatttt atctactgct
     5641 ccaccatata gatgagcatc aaggttctct agtaacagtc ctgtctgata gccacgaatt
     5701 cctcgattga caatcgtctt tgaagtccca aatagctcct gtagaggata atactcgaca
     5761 atggaatccc caataaaaag aatgtttggt tctacaacag aaatgtgatt taggtgacga
     5821 tacttagttt gaattttttc ttgttccttt aggagccaat tttctaataa ctgtactgcc
     5881 acttcattct cctttttcta accagttttc caagacttga taaactcctc cttggctgtt
     5941 ggctggtgct agagcagtcg ccacagcttt ggctttctca tcagcatttt ccatcgcata
     6001 ggcaattcca gccatttcaa gcatttcaac atcattttca ctatcaccaa aagccatgat
     6061 ttcttgggat ttcaagtccc agcgcttgag taattcctcc aagccccatg ctttatgaat
     6121 cccagcttgg aggatatcaa tgcaaccata gccactggat acagctcgga cacggccatc
     6181 aaagagagca ttgatttctt ccaaaaccga actcaaacgt tcctcaccaa caaccatgct
     6241 catcttgagc acaccaccaa agaggtcaga tgttaattca tccacaaatt gcatccgttg
     6301 gtagaatttt tcaatcattt ctggagtcat aaaactttca agatctgtaa aaatcgtacc
     6361 ttccttgaca aaatcaccct tcatccccgt tacaacaaac tggtcctgac acgctcgacc
     6421 cttgaaatga gtcaaagcct tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa
     6481 ttcattgttt tcaaaaatac gagcgccatt agcaacaacc agaaccactc gatccaccaa
     6541 gggactcagt agttgtctca tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat
     6601 gcccctttga tccaactgat ctaaaatctt ttccaatcgt gggagatcaa gctgacctct
     6661 agcatccagc aaggtcccat ccatatctgt cgcaattacc ttaatcatca tttactttcc
     6721 tctggattca agctaatacc acttcaatcc cacatgttca ttcatttctt tataggctgt
     6781 atcaattcgt tccttagtcg cttcatctaa ctggtcacga tgactgccat cctctataaa
     6841 atcttccaaa atataagttt ggtaatgata caagggatat ccttctggtg aaaaggttcc
     6901 ctttggtgtt cgattgaccc aagtaggatg agctttagct gttccgatag ttgtttttcc
     6961 atccttcttc ttgatggtga catccatgag aacaccacgt tcagtccact tagcattctc
     7021 ttcatctccc atagattcaa ttcgttgatt ggaaatgaag ttccccattt aatagataat
     7081 gagtttctta tctccatctt tttcaaccgt ttcagatggt tcaacaacgt gaggatgccc
     7141 tccaaagata atatccgctc ccaaatcgat catcttgtga taaagagctt tttgttcttc
     7201 agttggttcc aatcgatact caacacccat ctgaagcatg ataatggtga tatctgcttc
     7261 cttctctgcc cgttcaattt caaccttcat cttatcttcg tttaaatctg aaagataacg
     7321 attatagtct tcctgagaaa tatactgctc aattccattg aaaccatagg aataggctaa
     7381 caatgcaacc ttgataccat tcacttcctt aatgaccagc ggagcctgat cacgtggttc
     7441 gtgcgtataa actccgattg gagtgattcc agctttctca ataatatcgg ccgttgaaat
     7501 aactccctca atttgcgaat ccaaaatatg attatgagct aaatccagca catgataacc
     7561 tgcctcctta atagcatcca taacttcaac aggagcatta aagagaagat aacccgctaa
     7621 ataatgatcc ttattaatgg ttccttcaaa atcaccaata gctaaatctg ctgcttagtc
     7681 cccttgcttg agccaaggag tcacatactc aaaattttca tgaaagtcat aggtaccgtc
     7741 ttctttttta gctgaaaaga aaagtccatc gtggtagagc aaatccccgt gggccataat
     7801 tctggcagtt ttttcctctt cctactcctg agacttttgc ttagtcccct cttgagaaat
     7861 agtatctcgt ttttgactag tcaagggatt ccttggaagc tttcaaacaa caagaccaag
     7921 cccattgata aaccaactgc tagcaagagt atcgccacaa atcccttatt gctccacttg
     7981 cgataactcc taaaaagttt accaagccct tcataaaacg aaaagctaaa ccaccctgat
     8041 ttcgattttg tcttctttgt atcttcgttc tccctacttt cttatgcaag ccttttcttt
     8101 ttattatatc acagataagt atttctttca caattgaatt gaacttccca tctattttct
     8161 ataaatccta aatgccataa tgctttcaat tcttgtcatt ttgtgatatc atgtagagga
     8221 aatgaactaa tccacagtgg cttattccaa gtataccact tgggctttgg cagtagctaa
     8281 ctgcactaaa cataatataa ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg
     8341 ctcacttaat tgatatgaac gatattatcc gtgaagggaa tcctactcta cgcacggttg
     8401 ctgaggaagt cactttcccc ctatctgacc aggaaatcat cctaggcgaa aagatgatgc
     8461 aattccttaa acattcccaa gatcctgtca tggctgaaaa aatgggactc cgcggtggtg
     8521 ttggactggc tgctccccag ttagatatct caaaacgcat tatcgctgtt ttggtaccta
     8581 atattgttga agaaggcgaa actccacagg aagcctacga tttggaagcc attatgtaca
     8641 atccaaaaat cgtctctcac tctgttcaag atgctgctct tggcgaagga gaaggttgcc
     8701 tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt actgttgact
     8761 actttgacaa agatggagaa aaacaccgta tcaaactcaa aggctacaac tccattgttg
     8821 ttcagcatga aattgaccac attaacggta tcatgtttta cgatcgcatc aatgaaaaag
     8881 acccatttgc agttaaagat ggtttactga ttcttgaata aagaaaatcc cgttgcaaga
     8941 cggggttttg tgttataata gaggcatgaa aacaaatgat attgtctatg gtgtccacgc
     9001 cgttaccgaa gctctccttg caaatacagg aaacaaactc tacctccaag aagatctccg
     9061 aggtaagaat gttgagaaag tcaaggaact agctacagaa aagaaggtgt ccatttcttg
     9121 gacatcaaaa aaatctctct ctgagatgac tgaaggtgct gttcatcaag gttttgttct
     9181 acgagtgtct gaatttgcct atagcgagct agattacatc cttgcaaaaa cacgccaaga
     9241 agaaaatcca cttctattga ttctagatgg tctaaccgat ccccataatc tgggttctat
     9301 cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc attcccaagc accgtgctgt
     9361 cggagtaact cctgtcgttg ccaaaacagc cacaggtgct attgaacacg ttccaattgc
     9421 ccgagtgaca aacctcagtc aaaccttaga taaactcaag gatgaaggct tctggacctt
     9481 tggaacggat atgaacggta ctccttgcca caagtggaat acaaaaggga aaatcgccct
     9541 catcattgga aatgaaggaa aaggtatctc tagcaacatc aaaaaacaag tcgatgagat
     9601 gattaccatt ccgatgaatg gacatgttca aagccttaat gccagtgttg ctgcggccat
     9661 tctcatgtac gaagttttcc gaaatagact ataaaaaagt ttccagtcat ctgattggaa
     9721 acttttttat gattaactat gttctgtgat aaacttgtag gcttcttgac cagcgatagc
     9781 tccatctcca actgctgttg ttacttggcg aaggtctttc aagcgaacat ctccaactgc
     9841 aaagataccg tcgactgcag tcttcatgtg gctatctgtc agaatccagc ctgcctgatc
     9901 ttggatattc aattctttaa caaaatcgct aagagggtcc aaaccaacat agataaagac
     9961 accaccgaag gcttgttctg tcacttgacc tgttttcaca ttttcaaata cgactgattc
    10021 tactcggttt tcacccttga tttcccttac tacagaatcc cagataaagc tgattttttc
    10081 attcgcaaag gcgcgttctt gtaaaacctt ttgggcacga agttggtcac gacggtgaac
    10141 aatggtaaca gtcttagcaa aacgagtcaa gaagagggct tcttcaacag ctgaatctcc
    10201 accaccaact accaataaat cttggtcacg gaagaaagca ccatcacaca cagcacagta
    10261 agaaacacca cgactgttca gttcttcttc tccaggcact cccaaaggac ggtgtttaga
    10321 accagttgct acgataactg tacgtgtttc atatgtttgg tcatcagtca tcactttctt
    10381 aaaatcacca tggtcttcga cattttcaac ataaccataa atgtgctcca caccaagatt
    10441 ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca ctaatattag cgtatcctgg
    10501 gtaattttcg atatcagatg tattattcat ctgaccacct ggcagaccac cttcaatcaa
    10561 agctactttt agattgcttc gagcagcata caaggccgca gtcatccctg caggtccagc
    10621 accgataata atagtatcgt acatatagat tccttctttc ttgttgtaac tatctttatt
    10681 ctaactcttt cttgtcaatc acgcaaggat tatgccttga aaacaagaat taaactcata
    10741 accgcaaagg ataatactgg gacaaccaag actccaatca taatcatatc atagagatgg
    10801 gcttggcgag ccttggctgt cttatcaact cccgacatgg ctctcagacc aatccaaatc
    10861 cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac tctcgaaata taaagaaaat
    10921 agttgcagta gcatgatttc tctcatttct atctttttta aagagtaaac tcagctagtc
    10981 caactaactg agttttcctt tatctattat atcaaatata agtccgtttg taactagcga
    11041 agaattcttt tgtccgctct tctttagggg tgtggataat ctcatccggt gttccagact
    11101 cgatgatttt ccccttatct aagaagagaa tcttatcagc cacttgggct acaaaggaca
    11161 tgtcatgact aaccaaaatc atggtttgac ctgacttagc agcatctgca atagactttt
    11221 ctacttcacc aaccaattct gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat
    11281 ctggtttcat agcaagcgca cgcgctaggg caacccgttg cttctgtcca cctgataaat
    11341 ggcgaggata atggttttca cggtccgaaa gcccaacctt agccaactct tccttggcaa
    11401 tcttagtcgc ttcttggtca gataatttct tgacaacaac caagccttct ttcacattat
    11461 caagtgctgt tcggcgttca aacaaattaa actgttggaa aaccatagac aacttacgac
    11521 gtagggcaag gatttcttct tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct
    11581 gaatagagcc actgtcaggt gtttcaagat aattgagact gcgaagaaag gttgattttc
    11641 cagctcctga agaaccaatc aaggctacaa cttccccttt ttgaatatcc aagtccagat
    11701 gatccaagac agtctgtcct gaaaaggatt tgcttaaatt tgaaatctta atcattaacg
    11761 aaggtctcct ttcacatctg tttgcactgt atcaggtgca gaaatagcca tttttctctc
    11821 gatgaaacga ccgaggcttt caattccgat attgaccacc caataaacaa gggcaacgga
    11881 gatgaagcgt tcaaaatagc gataatcagc tccacctaga atctgagctt gggcaaagac
    11941 ttccacaaca cccgcactaa aagctagaga tgttcccttg gtcaaaccga tgagggaatt
    12001 aatcaaggtt ggagtagcta ccaccgctgc attaggaata atcactcgtc gataaacttg
    12061 cgctcgggtc atacccagac tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat
    12121 ggctgcacga atggtttcac tagcataagc tgcctcatta aaggcaaagg cgacaatcgc
    12181 aaaagctgca gctggaatcg cattgatatt gagaccagtt ccccattgct gattgagggc
    12241 tttcaaagcc aaagggattc cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc
    12301 ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa atcttgacac gattgatctt
    12361 cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat
    12421 aattgttgtt ggaagttgtt ggacaattct agggattcca tcaaagaccg aacgtaggct
    12481 aaacagcttg ccatccggaa tcaattgcat caagttttgg taccaatctg atgctaaaat
    12541 cgttgtaaca ttcataaaaa catcctcttc tgataatgat tcattctacc atgcttctgc
    12601 cgtcaatgaa attatttgct acggtcagat acagactttg tctacctact agtttatcat
    12661 actttgaaac agggaagtta tatattttat ctatcaaaga gatagataaa aactatttca
    12721 gtcccagttc ttcataagat tttcgataac cgatttgctt aacagttcca ttttctacta
    12781 aaatgggccg ttttaacaac ataccgtcac ttgctagcaa ctcagccgct tctcggtttg
    12841 acaaacttcc taccttatct tttagcccta attcacggta tttgatccca ctggtgttga
    12901 aaaattgctt caattcaaat cctgaggttt ctagccaatt caaaatgact tcttggctag
    12961 gtgtttcttc cacgatatgg acggctttat agtccacacc taattgattt aattcttgtt
    13021 ttgctttttt acaagttgaa catttggggt attcgataaa ttctaacatg tgcttctcct
    13081 tctattgttt ctatctttaa aatctactcc ttcatgctcc aagagccaag ctttcttttc
    13141 cactcctgca gcatagcctg tcagacgctt gcctgctccc aacacacgat gacaaggtac
    13201 taggatagac caaggattgc gtcccactgc tccaccaatt gcttgagcag aagccacttg
    13261 caggtcttga gcaatttgtc cataggtcac tgtctgacca taaggaattc cctgtaaata
    13321 ggaccaaact ctcttttcaa aattcgttcc aattggcgcc aagagcaagt tggataaatc
    13381 ctgaggctcg cctttaaagt aatcatctaa gcaagcaata actgggtcta aaataggatg
    13441 actaacaact tcttctatcg tttcatctcc tagtcccctc tcaaaatgct tctgctcctg
    13501 aacccaaatt ccatacaaat aatggtcatc agctacaagt gatagaatac caattggtga
    13561 ggagtagagg atttttacat acttcttttg cattatttct ttaatttttg ataggccaag
    13621 cgttcgccat ctactggcac cttaccaact tgtttaaaac caagtttttc aaaaatatgt
    13681 tgcataacct tgttttcagc atgcgtatct gagcgaaaat caagataatc aaaaccttca
    13741 atcaagccct ctaagaaggt ttgagcaact ccttttccct gcacatctgc tgccacagca
    13801 atacgatgaa agactagata ctctgactct ccagcttgcc agtttccctc ataaatagct
    13861 tcataggcct cctctggact cttggtcaca gcagcatagg ctagcagttc tccctcttcc
    13921 aaggctacgt aggcttgccc tgagatgata tcatcgataa taacatctgc atttggatag
    13981 ccattttgcc actggtcact accagcatct gctaaacatt ttttggcatc ctccatcacc
    14041 tgcatgatgg catctacttc atttggaaaa gctaaacgaa tttccatctt ttttcctcat
    14101 ttctgactag tttctcccat catagcataa tttaagtatt tctacaagca gttaaaactt
    14161 gactttgtgt ttttgttatt ttataatcta gttatcaaaa ctatataaaa aggagttgaa
    14221 aatgaaaact accttttcct acccaaaatg ggcagaaatt ccaaacattg acctctatct
    14281 ggaccaggtt ttgctctatg tcaatcaggt ctgcgcccct atctctccta ataaagacaa
    14341 gggcctaaca gcatctatgg tcaacaatta tgtgaaaaat ggttacctga caaagcctga
    14401 caaaaaaaaa taccaacgcc aacagattgc ccgtttgatt gctatcacaa ccctcaagtc
    14461 tgtattttct attcaagaaa tagcccagac acttaatact ctacaaactc aagcaagttc
    14521 agaccaactc tacgatgctt ttgtggacta catgaaccaa ggaattgacc cagctaaccc
    14581 tattatccaa accagctgcc aaaccgtcaa actctatcat caaactctag acttaatcga
    14641 tcatactcaa gaggaggtaa tccaatgaac actagtctta aactcagcaa acaactcagt
    14701 tttggagagg agattgctaa tagcgtgacc catgctgtgg gtgcagtcat catgcttatc
    14761 ttgctgccta tttcatccat ctatagttat gaagcacacg gatttttatc ctctatcggc
    14821 gtttccattt tcgtcatcag tctctttctc atgttcctat catccaccat ttatcactct
    14881 atggcctatg gttcgaccca caaatatgtt ttgcgaatca ttgaccattc tatgatttac
    14941 gttgccattg ccggctcata cacgcccgtt gtcttgacct tgatgaataa ctggtttggc
    15001 tatctgatta ttgtcatcca atggggaacg accatctttg gtattctcta taaaatcttt
    15061 gctaaaaagg tcaatgagaa atttagcctt gctctttacc tgattatggg ctggttggtt
    15121 ctggctatca ttcctgccat tatcagtcaa acgacacccg ttttctggag tctcatggta
    15181 actggcggac tctgttatac agttggagct ggattttatg ccaagaaaaa accttatttc
    15241 cacatgattt ggcatctctt tatcctagct gcgtccgcac tccaatacat cgctattgtt
    15301 tattacatgt aaaactctaa gcaggcagag catgcttaga gttttacaat cgaatttccc
    15361 aaagaggaag caagatgaaa gccatcattg cttgcgcaca taggattctc agaatcattt
    15421 acaaactact ctccaccaag cagactgatc aaacagaaaa ggcgctagaa ctgaggaaac
    15481 agttctaacg cccaaattaa aaaaatttca attacactat agcacaggaa gtgatttttt
    15541 gcgctttttt actgtctttt ttcaaataaa aaagt
//
LOCUS       DAVMEF010000034        15440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMEF010000034 DAVMEF010000000
VERSION     DAVMEF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.034"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      21..109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            324..992
                     /locus_tag="VYQ30_000766"
     CDS             324..992
                     /locus_tag="VYQ30_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1965919.1"
                     /translation="METQNYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     gene            complement(1142..1228)
                     /locus_tag="VYQ30_000767"
                     /pseudo
     CDS             complement(1142..1228)
                     /locus_tag="VYQ30_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1319..2101
                     /gene="thiM"
                     /locus_tag="VYQ30_000768"
     CDS             1319..2101
                     /gene="thiM"
                     /locus_tag="VYQ30_000768"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1965920.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            2103..2732
                     /locus_tag="VYQ30_000769"
     CDS             2103..2732
                     /locus_tag="VYQ30_000769"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1965921.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2941..3029
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3291..3851
                     /locus_tag="VYQ30_000770"
     CDS             3291..3851
                     /locus_tag="VYQ30_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1965922.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3852..5237
                     /locus_tag="VYQ30_000771"
     CDS             3852..5237
                     /locus_tag="VYQ30_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1965923.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5239..5889
                     /locus_tag="VYQ30_000772"
     CDS             5239..5889
                     /locus_tag="VYQ30_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1965924.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5900..6592
                     /gene="tenA"
                     /locus_tag="VYQ30_000773"
     CDS             5900..6592
                     /gene="tenA"
                     /locus_tag="VYQ30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1965925.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6597..7121
                     /gene="thiW"
                     /locus_tag="VYQ30_000774"
     CDS             6597..7121
                     /gene="thiW"
                     /locus_tag="VYQ30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1965926.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            7121..7924
                     /locus_tag="VYQ30_000775"
     CDS             7121..7924
                     /locus_tag="VYQ30_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1965927.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLNYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            7917..8549
                     /locus_tag="VYQ30_000776"
     CDS             7917..8549
                     /locus_tag="VYQ30_000776"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1965928.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8758..9549)
                     /gene="thiD"
                     /locus_tag="VYQ30_000777"
     CDS             complement(8758..9549)
                     /gene="thiD"
                     /locus_tag="VYQ30_000777"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1965929.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQAKDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9633..9731)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9915..10310
                     /locus_tag="VYQ30_000778"
     CDS             9915..10310
                     /locus_tag="VYQ30_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1965930.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10321..10692
                     /locus_tag="VYQ30_000779"
     CDS             10321..10692
                     /locus_tag="VYQ30_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1965931.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            10702..12945
                     /locus_tag="VYQ30_000780"
     CDS             10702..12945
                     /locus_tag="VYQ30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1965932.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13152..14927
                     /gene="spxB"
                     /locus_tag="VYQ30_000781"
     CDS             13152..14927
                     /gene="spxB"
                     /locus_tag="VYQ30_000781"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1965933.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            15038..15385
                     /locus_tag="VYQ30_000782"
     CDS             15038..15385
                     /locus_tag="VYQ30_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1965934.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         4546 a         2827 c         3416 g         4651 t
ORIGIN      
        1 ctggttcagt aattaaataa tagagacatt tggggtgctt taggctgaga taatacccat
       61 tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa acgttttttc gtatggcgaa
      121 atgaacggtc taatcttgta agtcaactct aattcttgat tgtaattgga gttttttgtt
      181 tatataaact ggataggctt gtttagttag ctctctgcaa atgctctcga tgtgctttaa
      241 aaggatcaag tggaattacg attttacttg atatttcttg gtgttttatt aattactaac
      301 aagtaaagag aggagaagta aatatggaaa cacaaaacta tgcatttcag ccaggtttga
      361 ctgttggaga attattaaaa agcagtcaga aggattggca ggctgcaatc aatcatcgtt
      421 ttgttaagga actttttgcg gggacaattg agaataaggt cttaaaggac tatctgattc
      481 aagattatca cttctttgat gccttcttat ccatgctggg tgcctgcgta gctcacgcag
      541 ataaacttga atccaagctt cgttttgcca agcaactagg ctttcttgaa gcagatgaag
      601 atggttattt ccaaaaggct tttaaagagt taaaagtagc agagaatgac tatctagaag
      661 tgactttgca ccctgtaaca aaagcctttc aggatctaat gtattcggca gtggcttcat
      721 cagactatgc ccatcttttg gttatgctgg tcattgcaga aggtctctat ttagactggg
      781 gttctaaaga tttggctcta cctgaagtct atattcattc ggaatggatc aatctccaca
      841 gaggtccttt ctttgcagag tgggttcaat ttctggttga cgaactcaat cgtgtcggta
      901 aaaatcgaga agatttgaca gaacttcagc aacgctggaa tcaagcagtc gctttagaat
      961 tagctttttt tgatattggt tacaatgtct agagaaggct catgatattt acaaatttga
     1021 tgaggtaaga ctcttcgaaa atctcttcaa acaacgtcag tttcaccttg ccgtaggtat
     1081 ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     1141 cagtcttatc tacaggttca aagcagtgtt ttgagcaacc tgcggttagc ttcctagttt
     1201 gctctttgat tttcattgag tataaatcat tcaatgtaaa tgaaaaaaat ttcttaacga
     1261 aagatagata taaatcgaaa tcagtagatc gtataaagtg aaaaggaagg atttcaatat
     1321 gacaagttta aaattattaa aagaaaaagc accattggtc atttgtataa ccaatgatgt
     1381 agtaaaaaat ttcacagcaa atggattagt agcactgggc gcctcaccag ccatgagtga
     1441 gtttccagca gatttagagg atttgttaaa gtatgctggt ggtttattaa taaacatagg
     1501 aacattgaca gatgaaaatt ggaaattata ccaagctgct ctgaaaattg cggagaaata
     1561 taatgtccca gcagttttag atcctgtagc ctgtggagca ggagaatata gaaaaaaagt
     1621 agcggatgat ctaatcaaca attataaact agcagcgatt agaggaaatg ctggcgagat
     1681 tgcctcttta gtaggaatag atgtggcatc taaaggagta gatagtgcgg gcgtagataa
     1741 tattgacgaa attgctctag cagcaaatga gaagttcaat attccaatag tagtaacagg
     1801 tgaagtggat gccattgctg ttaatggaga agtggtaacg attcataatg gtagtgctat
     1861 gatgccgaaa gtcattggga caggatgctt attaggagct gtagtagcaa gctttatcgg
     1921 actagaaaaa ggtcaagaat tgaaatcatt agaaacagca atgttggttt acaatatcgc
     1981 tggagaaata gcagaaaaac gtcccaatgg acatcttcct ggaacattta aagctgaatt
     2041 tataaatgcc ttatacgaga ttacagatga agatgtaaag gaattcaaaa gagtgaagta
     2101 agatgtttca taaagaatta ctaaaactat attttatttg tggaacgact acttgccaag
     2161 gaaaaaatct atatacagtc gttgaggaag ccttaaaagg tggtataacc ttatttcaat
     2221 ttcgagaaaa aggtgagggt gctttagaag gcttagaaaa acttgagcta gctatccaaa
     2281 ttaaagaact ttgtaaaaaa tacaatgttc catttatcgt taatgacgat atagatttag
     2341 caatggaaat tgacgccgat ggcgtacatg taggtcaaga tgatattggt gttgatgaaa
     2401 ttagaaaatt gatgccagat aaaataattg gtctttctat aagaaacgaa gaagaatttc
     2461 aacaatcaaa agttgaatat gtagattatg taggtgttgg gcctgtattt gatacccagt
     2521 caaaagatga tgctggtggt gctataggtt atgaaggtct tgaattgatg agaaaactat
     2581 tgccacaaat gcctttagtt gcaattggtg ggatacagac gaaacatatt aaagacatta
     2641 taaagaccaa tgttgacggc gtttcaatca tttcggcaat atcgtatgca aaaaatatag
     2701 aaaaaactgt tcgggaaatg agtgaacaat agatatctat ccttggattc taagtaagta
     2761 ggattttcct tttttaaaaa gctatgtcta gattcagctt gaatggctaa ttcatcaaac
     2821 aggtcatttg ttttttgcaa aagaaagatc tgctgtcttt gtgagtgcaa ggctgtattt
     2881 ccagttcaat ttcttgaact agtataaaat gtgtgttata ataatcacat aattaaataa
     2941 taaaggcatt tggggtgctt taggctgaga tgatacccat tgaacctgat acagttaaga
     3001 ctggcgaagg gaaatgtgaa atgttttttt cgtatgtcga aaagaactgt ctaatcttgt
     3061 aagctgaact ctaattcgta tctgtaatta gagttttttc ttttacgaaa ctggatgaag
     3121 atgatatagt agattgaaat aagatgcgaa caaatcaatt agaaaattca aattaatttc
     3181 tagaaatatt ttagcattcc tgtgtactgt tctaaattca atctgctata caaagtattt
     3241 aggtcggtct tcttgagtgc cccacttttc ttttaaaaaa ggagtttttt atgttgaaaa
     3301 aatggcagtt aaaagatgtt atcttgcttg ctttcttgtc tatctttttt ggtggggttt
     3361 tcgttggttc aggatatgtg tataatattc tcagtctact cttaacacct cttggtttgc
     3421 aggcctttgc caatgaaatc ctcttcggtc tctggtgtat ggctgcgccc attgctgcca
     3481 tctttgttcc gagagtcgga agtgcaacga ttggagaagt gctagctgcg cttgctgaag
     3541 tcctttatgg tagccaattt ggtctaggag ctcttttgtc tggcttggtt caaggtttgg
     3601 gaagtgaatt tggttttatc gtaactaaga atcgctatga aagttggctc tctctaactg
     3661 ctaatagtat tgggattacg cttgttagct ttgtctatga atacattaag ttaggttact
     3721 acgccttttc ccttccgttt gtcctttcct tacttgtggt acgttttatt tctgtttatt
     3781 tcttctgtac catcttggtt cgtgccattg tcaaactcta tcatcagttt gcaactggag
     3841 gaaaagcata gatgggtctg gaactacgag cgattcagtc cccaatcttc tctgagccgt
     3901 ttgattttac ttttcatgcg caagccttta ccttgttagt tgggagcagt ggttcaggaa
     3961 aatccagcct ttttcaagtc attgcccaag tcagttctct tccctatagt ggtcaagtcc
     4021 tgatagatgg gagcgaggtc agtcagcttt ctatcatcgc acgtgtccag aaggttggca
     4081 ttctctttca aaatcccaat catcaattta ccatggagaa cttgtttgag gagctgattt
     4141 tcaccttgga gaatatcggc tatcaccttc aggaaattga ttctaaaata gcagaggttg
     4201 tccagcaatg tcgttgcgag gcaatcttac accgtcccat tcatcaccta tcaggtgggg
     4261 aaaagcaaaa agcagcgctg gctgtcctct ttgccatgaa tcctagggtc tatctcttgg
     4321 atgagccctt cgcttctatt gaccgcaaga gcagaatcga gatattggag attctaaaag
     4381 agttggctct tgatgggaag acagttattt tgtgcgacca tgatttatct gactataaag
     4441 cctatatcga ccatatggtt gagctaagag acggaaaact aagggaagtg tttcaaatcc
     4501 cttcctatga gatgacacag gttgcttcaa aggaagttgc ttctagcccg gaactattcc
     4561 atatgaaccg tgtgactggt gagcttggta atcgccccct cttttcaatt gctgatttca
     4621 cattctatca agggatttcc tgtatcctgg gtgacaatgg tgtcgggaaa tcaacccttt
     4681 ttcgctctat tcttcaattt caaaagtata aggggcgcat tgcatggaag gggacagtcc
     4741 tgaaaaagaa aaagagtttg tatcgtgatc tgactggtgt tgttcaggaa gctgagaagc
     4801 agtttatccg agtcagtctg cgagaggagc ttcaattaga tggacctgat tctgaaagaa
     4861 atcagcggat ttttcaagct ttacgatatt ttgatttgga gcaggcagtc gataagagtc
     4921 cctatcaatt aagtggtggt cagcaaaaaa ttcttcagct cttgaccatc ttgaccagta
     4981 aggcttccgt gatcttgcta gatgaacctt ttgcaggttt ggatgataga gcctgccatt
     5041 atttttgcaa gtggattgtg gaggagagga atcaaggaag aagttttctg ctcattagtc
     5101 atcgtttaga ccctttgatt tctgtggttg attattggat tgagatgact agtcagggtc
     5161 ttcggcatgt gaaagaagtg accattacca aaccacttac atctcagagt agcaataccc
     5221 aaggggaggt gagatagtat ggtcaaagta gcaacccaga caccgattat cagtctcttc
     5281 ttgctgattt tatccttgga aacatctttc attccttcga ttgctctgaa tctttcggta
     5341 gtcgcatttt gtattctctt tatgctctat taccgtcgat ttaaaatgtt agcttggatg
     5401 atcatacttg ccattttacc atcttttgcc aactactggg cagttcagtt acacggagat
     5461 gcttcacagg cagtcatgct tggaacgagg gcctttgtga cagtttgtat cggccttgtc
     5521 tttgtttcct ctatttcact aaaagagctt ctcttgtact tggctcaaaa ggggctatca
     5581 cgctcttggt cctatgcctt gattgtggta ttcaattcct ttcctctcat tcagcaagaa
     5641 atcaagtccc tcaaagaagc ttgcctatta cgtggtcaag aactgcattt ttggtcgccc
     5701 ttgatttaca gtaaggttct gatgacagtc tttaggtggc gccatcttta cctgagagct
     5761 ctgtctgctc atggatatga cgaacatgca cagttgaaga atagctatcg gactttttat
     5821 attcctaaaa aaacaaaatt aatctacctg cttttctttt tattgcttca aaccagtcta
     5881 tttttataaa ggagttatta tggaatttac agatattgcg atggaattat ccaagaaagc
     5941 ttggcaggct tcctttcatc atccctttat tttacaatta caagagggga atttagaacc
     6001 tgccattttc cgctattacc tgattcagga tgcctactat ctgaaggcct tctcagaaat
     6061 ctatcatctt ttggctgata agacttcaaa ccaagagatg aaaagactct tgaaacaaaa
     6121 tgctcagggt ttagtggagg gtgagttatt tattcgccaa caatttttca aggaaatgga
     6181 aatcagcgac caggaaatgg agcaacatcc aattgctcca acctgttatc attacatttc
     6241 tcatatttat aggcaatttg cagaaccgaa cttagccata gcttttgcaa gcttgcttcc
     6301 ttgtccttgg ttataccatg atataggcaa atcacttaat cttaaaccat caccaaatcc
     6361 tctctatcaa caatggattg aaacttatat tacggatgag ttagagcaac agatcagaga
     6421 ggagggagca ctggtcaatc agctctatcg agaaagtgat gagacagata agcaaaaaat
     6481 gctagatgcc ttccacatca gtgttcatat ggaagccaag ttttgggaga tggcttacca
     6541 acaccagaca tggaagagcg atttacagtc tttagaaaaa ggagaagaat agaaacatga
     6601 gaaagcacca actacaagtt cacaaattga ccattttatc tatgatgatt gcccttgatg
     6661 tagtccttac acctatcttt cgaattgagg gaatggcacc gatgtccagt gtagtcaata
     6721 ttctagcggg aatcatgatg ggacctgttt atgccttggc catggctaca gtcacagcct
     6781 ttatccgtat gacgactcaa gggattccgc ctttagctct cacaggagcg acttttggag
     6841 cccttctggc aggtctcttt tataagtacg gtcgaaaatt ttatttttct gccttgggag
     6901 aaattgtggg aacaggtatt attggttcca ttgtttccta tcctgttatg gtactcttta
     6961 caggatcagc tgctaagctt agctggttta tctacacgcc tcgatttttc ggagcaacct
     7021 tgattggtac agcgatttcc tttattgcct ttcgattttt aatcaagcag gaattcttta
     7081 aaaaagtgca gggatatttc tttagtgaaa ggatagactg atgcaggaat ttacaaatcc
     7141 ctttcctata ggctctagtt ccctaattca ctgtatgacc aatgagattt cttgtgagat
     7201 gctggcaaat gggattttgg ctctgggatg caaacctgtc atggcagatg atccccgtga
     7261 ggttcttgat tttactaagc aaagtcaggc tcttttcatc aatttggggc atttgtcagc
     7321 tgagaaggaa aaagcaatcc gcatggcagc ttcgtatgca aaccaatctt ctctcccgat
     7381 ggtagtagat gcggttggcg taacgacttc atccattcgt aagagcttag ttaaagacct
     7441 tttaaactat agacctacgg tcattaaagg aaacatgtca gaaattcgaa gtcttgttgg
     7501 cttaaagcac cacggcgttg gggtcgatgc gagtgctaaa gatcaagaaa cggaggattt
     7561 gcttcaagtc ttgaaagact ggtgtcagac ctatcctggt atgtcattct tagttactgg
     7621 ccccaaggac ctcatcgttt cgaaaaatca ggtcgctgta ctggagaatg gctgtactga
     7681 attagactgg ataacaggga caggagattt ggttggagcc ttaacagccg tttttctcag
     7741 ccaaggaaag actggttttg aagcttcttg cttagcagtc tcttatctca atatcgctgc
     7801 tgagaaaata gttgttcaag gaatgggatt ggaagaattt cgttaccaag tactcaatca
     7861 gctttcgctc ctaagaagag atgaaaattg gctagatacc atcaaaggag aggcttatga
     7921 atagagaagc acttagacta tatctggtaa ccaatcgcta tcaagattcc gtggaaagct
     7981 ttcttgcaaa agttgagacg gcctgccgtt caggggttac catagtccaa ttgcgagaaa
     8041 aaaatctcac aaccaatcaa tattatcaac tggcaaaaca agtcaaggaa ataacagatg
     8101 cctatcaggt acccttgata atcgatgatc ggttggatgt ttgtcttgcg gttgatgctg
     8161 ctggtctgca tattggagat gacgaactcc cagtttcggt tgcccgacaa gtcttgggtc
     8221 ctgaaaaaat cctcggtgtc accgctaaaa cggttaaaag agctctggaa gcggagaaat
     8281 caggtgcaga ttacttgggg acaggagcca tttttccgac aaccaccaag gaaaatgcac
     8341 ccatcaccct gatttcaacc ttgaaaacaa tttgccaaac ggttgccatt ccaatagttg
     8401 ctattggcgg cttgacttcg gagaatattg atcagcttat gggcactggc atagctggtg
     8461 tagctgttgt acgtgatttg atgcaagcag aagatattga ggcaaaaacg caagcctttt
     8521 taacaaagtt gcatgacatt ctttcctaat taatactcaa tgaaaatcaa agagtaaact
     8581 agaaagctag tcgcaggttg ctcaaagtac tgccttgagg ttttagataa gactgacgaa
     8641 gtcagctcaa aacagtgttt tgaggttgca gatggaagct gacgtagttt gaagagattt
     8701 tcgaagggta taaaaactct ctaattccct taaagtggga actagagagt ttttttctta
     8761 atctttaaaa gttgtatggt tgactggacc agaaccatga ccgagttgag gggcatcttg
     8821 aatagctttt gtgataaagg ccttggcctt atcaactgcc tggtaaagac tcttgccctt
     8881 ggctagttca gcagtaatca ctgcagcaaa ggtacatcca gtaccatggg tgtgacaggt
     8941 ttgaattcgt gggctttccc agacaaattg ttcattcttg gtaaagagga aatctttagc
     9001 accacctttg agatgtccgc ctttgataac cacagactga ggaccaaatt cttttaaaat
     9061 caggcgacca gcacgctgca tgtcttcggg gtcatggatt gaaaaaccaa caatctcttc
     9121 tgcttcagga agatttggcg taataatagt tgctagaggt agtaagtttg ttttgagata
     9181 gtctctagca tttgagtcaa tcaaggcatc tccacttgta gcaaccataa caggatcaag
     9241 gacataggga caatccagtt ttttaagata gggttggatg atttccatga tttcagtagt
     9301 agccaacatt ccagttttta cagcctgagg tggaatatca gaaaagacac tctccaattg
     9361 ggctttcaac atttgaggag aaacgtgctc gattagctga acacctctgg tattttgagc
     9421 gacaagactg gttacaacag ccattccata gacatctttc gcttggaatg actttaaatc
     9481 tgccataatg ccagcaccac cactagggtc agtccctgca atggtcaaag caacgggtaa
     9541 ataagtcatc taaaacctcc caaccaactg aagtttgtat tcttaagaac aagctagtct
     9601 gttttagaaa ggcaatagaa gactgctagt ccccattatc atttccactt ccatcagcta
     9661 gcattaccta gattgagtca gagggatcta acagtcgtta atctcagctt tacgcacccc
     9721 tagtggttgt tttctttttt ctttagtata gcatattttt attgtttata tagtaatatt
     9781 tgactgcaaa tcgtttttca aatgattaaa attctatttt tcgaagaaaa tattatttct
     9841 ataattgaca aatgtagatt ttaagagtat actgatgagt gtaattgaca aatgtagatt
     9901 ttggaggtat ggtaatgcag atttcagatg cagaatggca ggtcatgaag attatttgga
     9961 tgcaggggga gcagaccagt acagatttga ttagggtttt ggcagagcgg tttgactggt
    10021 ccaagtccac ggttcagaca cttttggctc gtctggttga gaaagagtgt ttgactcgga
    10081 aaaaagaagg caagtccttt gtttattcag cccttttaac tctagaccaa agtcgggatt
    10141 tacttgtcca agatatcaaa gacaaggttt gttcccgtag gattaggaat ttgttggctg
    10201 acttgattgt agaatgtgaa tttactcaga ctgacttgga agacttggaa gctgtgattt
    10261 cagagaagaa atcaagtgct gtaacagaag taagatgtaa ttgtatgtaa aggagacgtc
    10321 atgttaaata gtattgtaac cattatttgt attgccctta tcgcgtttat cttgttttgg
    10381 tttttcaaaa agcctgaaaa atctggacaa aaagcccagc aaaaaaacgg ataccaagag
    10441 attcgagtgg aagtcatggg aggctatact cctgagttga ttgtcctcaa gaaatcagtg
    10501 ccagcccgca ttgtctttga ccgcaaggat ccttcaccat gtctggatca aattgttttt
    10561 ccagattttg gtgtacatac gaacctgcca atgggggaag agtatgtagt ggaaatcacg
    10621 cctgaacagg ctggagaatt tggctttgct tgtggtatga acatgatgca cggcaagatg
    10681 attgtagagt aggtggagac tatgacagaa attgtgaaag caagcttaga aaatggcatt
    10741 caaaaaatcc gtatccgatc tgaaaaaggc tatcatccag cccatatcca gcttcaaaag
    10801 ggaattccag ctgagattac ctttcatcgt gctactcctt caaactgtta taaggaaatt
    10861 ctgtttgaag aagaaggtat cttggaacca atcggcgtag atgaggagaa agtcattcgt
    10921 tttacacctc aagaattagg gcgacatgaa ttttcttgtg gcatgaagat gcaaaaggga
    10981 agctatacag tcgttgagaa gactcgaaaa tctctatctc tcctgcaacg tttttggatt
    11041 actagtatct ttactgtgcc tcttgtgatt ctcatgattg ggatgttgac aggtagcatt
    11101 agtcatcaag tcatgcattg gggaaccttt ctagcaacaa cgcctattat gttagttgca
    11161 ggtaagccat atatccagag tgcttgggcc agttttaaaa agcacaatgc caacatggat
    11221 accttggttg cgctgggaac tctagtggct tatttctata gcctagttgc tctctttgct
    11281 ggtctccctg tttacttcga aagtgctgga tttatcctct ttttcgttct tttgggagca
    11341 gtttttgagg aaaaaatgag aaaaaatacg tcccaagctg tggagaaatt actggacttg
    11401 caagctaaaa ccgcagaagt cttgagtgat gatagttatg tccaagttcc tttggaacaa
    11461 gtcaaggtag gcgacctgat tcgagtgcgt cccggtgaaa agattgctgt tgatggtgtc
    11521 gtagtagaag gtgtctctag tattgacgaa tccatggtga caggtgagag tctgcctgtg
    11581 gacaagacag ttggagatac tgtcattggc tcaaccatca atcatagtgg aacgcttgtc
    11641 tttagagcag aaaaagttgg ctcagagact gttttggctc agattgtgga ttttgtgaag
    11701 aaagctcaga caagtcgtgc gccgattcag gacttgacgg ataagatttc agggattttt
    11761 gtcccagtag ttgtcatttt aggaatcatg accttttggg tttggttcgt cttgctcagg
    11821 gatagtgtgg ttgtgcttgg agctagcttt gtgtcctctc ttctctacgg agtggcggtt
    11881 ttgattatcg cctgtccttg tgccttggga cttgcaacac cgacagccct tatggtgggg
    11941 acaggacgta gtgccaagat gggggttctc ctcaaaaatg gaactgtctt acaggaaatc
    12001 cagaaagttc aaacccttgt ctttgataag accgggactt tgacggaagg gaaacctgtg
    12061 gtaacagatg tcatcggcga cgaagtagaa gtgtttgggt tggcagcctc cttggaagat
    12121 gcttctcaac acccactggc tgaggctatc gttaagcgag cgagtgaagc tggacttgag
    12181 tttcaaactg ttgaaaattt tcagaccttg cacgggaaag gtgtttcagg gcgaatcaat
    12241 ggaaaacaag ttttacttgg aaatgctaaa atgctggatg gcatggatat ttctaatact
    12301 tatcaagata aactagaaga actagaaaaa gaagctaaga cagttgtgtt cttagctgtt
    12361 gacaatgaaa tcaaaggctt gcttgctttg caagatattc ctaaggaaaa tgctaagcta
    12421 gccatcagtc agctaaaaaa acgtggtctc cgaacagtca tgctgacagg agacaatgct
    12481 ggtgtggcgc gtgctattgc agatcaaatc ggaattgaag aggtcattgc aggcgtcttg
    12541 ccagaagaaa aagcccatga aatccataaa ctgcaacagt cagggaaagt agcctttgtt
    12601 ggggacggta tcaatgacgc tcctgccctt agtgtagcag atgtgggaat tgctatgggt
    12661 gctggaacag atatcgccat cgagtcagca gatttggtgt tgacaaccaa taatctttta
    12721 ggagtggttc gtgcctttga tatgagtaag aaaacctttc atcgaattct actcaatctt
    12781 ttctgggctt ttatctacaa tgttgtcgga attccgattg cagcaggagt cttttcaggt
    12841 gttggactgg ctctcaaccc agaattggca ggtctagcca tggcctttag ttctgtatct
    12901 gttctgacca gttcccttct gttaaatttt agtaaaatag actaaaagta atgactagat
    12961 ttctttgtta taaaacagaa acgacaacac tttcaaaact gtattagaat agagaataga
    13021 gagttttgag cagattttta gaaaagtcag cataaatatg atacagtgga atagtaaaaa
    13081 tttggagaac gtttccaatt ctatgtaatc gtattctcca agtttaaaaa aattgaagga
    13141 gagttatcat tatgactcaa gggaaaatta ctgcatctgc agcaatgctt aacgtattga
    13201 aaacatgggg cgtagataca atctacggta tcccatcagg aacactcagc tcattgatgg
    13261 atgctttggc tgaagacaaa gatatccgtt tcttacaagt tcgccacgaa gagacaggtg
    13321 ctcttgcagc ggttatgcaa gctaaattcg gcggctcaat cggggttgca gttggttcag
    13381 gtggtccagg tgcgactcac ttgattaacg gtgtttacga tgcagctatg gataacactc
    13441 cattcctagc gatccttgga tcacgtccag ttaacgaatt gaacatggat gctttccaag
    13501 agcttaatca aaacccaatg tacaacggta tcgctgttta caacaaacgt gtagcttacg
    13561 ctgagcaatt gccaaaagta attgacgaag cctgccgtgc tgcagtttct aaaaaaggtc
    13621 cagctgttgt tgaaattcca gtaaacttcg gtttccaaga aatcgatgaa aactcatact
    13681 acggttcagg ttcatacgaa cgctcattca tcgctcctgc tttgaacgaa gttgaaatcg
    13741 acaaagctgt tgaaatcttg aacaatgctg aacgcccagt tatctatgct ggatttggtg
    13801 gtgttaaagc tggtgaagtg attactgaat tgtcacgtaa aatcaaagca ccaatcatca
    13861 caactggtaa aaactttgaa gctttcgaat ggaactatga aggtttgaca ggttctgctt
    13921 accgtgttgg ttggaaacca gccaacgaag tggtctttga agcagacaca gttcttttcc
    13981 ttggttcaaa cttcccattc gctgaagttt acgaagcatt caagaacact gaaaaattta
    14041 tccaagtcga tatcgaccct tacaaacttg gtaaacgtca tgctcttgac gcttcaatcc
    14101 ttggtgatgc tggtcaagca gctaaagcta tccttgacaa agtaaaccca gttgaatcaa
    14161 ctccatggtg gcgtgcaaac gttaagaaca accaaaactg gcgtgattac atgaacaaac
    14221 tcgaaggtaa aactgagggt gaattgcaat tgtatcaagt ttacaatgca atcaacaaac
    14281 atgctgatca agacgctatc tactcaatcg acgtaggtaa cactactcaa acatctactc
    14341 gtcaccttca catgacacct aagaacatgt ggcgtacatc tccactcttt gcgacaatgg
    14401 gtattgccct tcctggtggt atcgctgcta agaaagacaa tccagatcgc caagtatgga
    14461 acatcatggg tgacggagca ttcaacatgt gctacccaga cgttatcaca aacgttcaat
    14521 acgaccttcc agttatcaac cttgtcttct caaatgctga gtacggcttc atcaagaaca
    14581 aatacgaaga tacaaacaaa cacttgtttg gtgtagactt cacaaacgct gactacgcta
    14641 aaattgcgga agctcaagga gctgttggat tcacagttga ccgtatcgaa gacatcgatg
    14701 cagttgttgc agaagctgtt aaattgaaca aagaaggtaa aactgttgtc atcgatgctc
    14761 gcatcactca acaccgtcca cttccagtag aagtacttga attggatcca aaacttcact
    14821 cagaagaagc tatcaaagcc ttcaaggaaa aatacgaagc agaagaactc gtaccattcc
    14881 gtctcttctt ggaagaagaa ggattgcaat cacgcgcaat taaataattc ctctcgccga
    14941 aaatcaaata tgaaacttgt agactttatt ctttgatttt catagagcag aactttaaaa
    15001 gatcggagca atccggtctt tttatggagg agagtaaatg aatttaaatc aattagatat
    15061 tatcgtttca aatgttcccc aagtctgtgc tgacttggag catatcttgg ataaaaaggc
    15121 agattatgct gatgatggtt ttgctcagtt tacgattggt agtcactgcc ttatgttgtc
    15181 acaaaatcat ttggttcctt tggaaaactt tcagtcagga atcattattc atatcgagat
    15241 tgaggatgta gaccagaact acaaacggtt gaacgagctt ggtataaagg ttttacacgg
    15301 accaactgta accgattggg gaacagagtc cttattagtt caaggacctg ctggtctagt
    15361 gcttgatttt tatcgtatga aataggtcgc gtagtcattt gacgttaacc ttagctattt
    15421 ttaaaataga aattgagaat
//
LOCUS       DAVMEF010000035        15378 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMEF010000035 DAVMEF010000000
VERSION     DAVMEF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.035"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(184..1044)
                     /locus_tag="VYQ30_000783"
     CDS             complement(184..1044)
                     /locus_tag="VYQ30_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1965935.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            complement(1223..3277)
                     /locus_tag="VYQ30_000784"
     CDS             complement(1223..3277)
                     /locus_tag="VYQ30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1965936.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            complement(3289..3543)
                     /locus_tag="VYQ30_000785"
     CDS             complement(3289..3543)
                     /locus_tag="VYQ30_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965937.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            complement(3536..4093)
                     /locus_tag="VYQ30_000786"
     CDS             complement(3536..4093)
                     /locus_tag="VYQ30_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1965938.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            complement(4077..5033)
                     /locus_tag="VYQ30_000787"
                     /pseudo
     CDS             complement(4077..5033)
                     /locus_tag="VYQ30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            5125..5484
                     /locus_tag="VYQ30_000788"
     CDS             5125..5484
                     /locus_tag="VYQ30_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1965939.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            5694..6550
                     /locus_tag="VYQ30_000789"
                     /pseudo
     CDS             5694..6550
                     /locus_tag="VYQ30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6798..13499)
                     /locus_tag="VYQ30_000790"
     CDS             complement(6798..13499)
                     /locus_tag="VYQ30_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1965940.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVKVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(13804..15279)
                     /locus_tag="VYQ30_000791"
     CDS             complement(13804..15279)
                     /locus_tag="VYQ30_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1965941.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT         4435 a         3350 c         2928 g         4665 t
ORIGIN      
        1 gatttattat actccttcca ttttcgaaaa aacagggaca atgatagggg aaaataggaa
       61 tttaatcaga tgatttagaa atcttggaca aaaactatac aaaaaactgt caaagcacaa
      121 aagcgcatag tatcaagatt ctataaagcc ttgatactat gcgcttttgt aatggataaa
      181 tagttagtct tttttaaaga ccggatcttt caaactctgc atactggcat tgatcaccgc
      241 gcctaggata acaattttag caatcaagat aaaccaaaac atcataacaa caagaagaac
      301 ggaacctaaa attcggacat ccaccaaatg atggacatag taattgagat aactagagaa
      361 cagagttagt aaacctaaaa tcactaagag aacaaaggca ctgcctggta gggtatagct
      421 aattttcctg ttagatagat tgggaagaaa ataataaagc atgaccaaga tagcaaagag
      481 gagggcgtaa atcagaggac ctgccaaccc ttgtaaagcc tgatagataa tgccatcttt
      541 tgtccaataa tgagcaagta aagccaaaat catctgacca aataagatca aaaacaaggc
      601 aaacgcaaag aggagctgca agccaaaact gactaggaga cttagcatct gatgggaaat
      661 aagtccacga ctcttttcga cgccataagc cttgttaaaa gctttttgca agaaattcat
      721 agattttgaa aaactccata acgccgataa aacagaaaaa ctcaataaac ctgttgaagg
      781 ttgcgtcaag acttctctgg ctattttttc cacaccttca tagaggcttg ggggcaggac
      841 gtctttcata aagcccaaaa attctcccac aggaatctga aaatagggga ggatattgac
      901 caccaccaaa agcaggggga aaatcgaaat caaccaatag taggctactg cgacactggt
      961 caactcacta tctgatgctt gataataatg caaaaaagct tttaataaag gtttgtctat
     1021 cagctctttc caccactttt tcatgtcata ctccttcatt tataatctta tactcaatga
     1081 aaatcaaaga gcaaactaga aagctagccg caagctgctc aaaacactgt tttgaggttg
     1141 tagataagac tgacgaagtc agtcacatac atacggtaag gcgacgctga cgtggtttga
     1201 agagattttc gaagagtatt aactaatttc ttcttaccaa ttccaccata tcatacggta
     1261 gggtattggc agcttccttc aaggaatagt tctctaagtt atttacattt tgtcgtaatt
     1321 tcttggcata cttagtcgta atcaatcgtt tttcttcgta ttcgaaaatc aacttgcgct
     1381 ccagataata gcctctcagc atttcatcga tattgttggg tttgacacga ttgataaccc
     1441 gttcgacaaa ggcaccactg ctgataatag ctgtttctcg aagacgagac tcctgcataa
     1501 aactaatcaa agagcgtctg tagactccct tcaggttttc caaactttca ataatcatct
     1561 ctgtattggc aagatagagc tctgcaattt ggtcataatc aagagcacgg agacggcttt
     1621 gctccttgtt cttccagcta cggaaggtct ttccgagagt aaaaacttca tgaaggagaa
     1681 aacgtaaaat ccgcaaagaa acaaggaaat aataggtcag tcttgaggca agtttacgat
     1741 tgattccttg ttctatattt ttcagataac gttggtaaac tcggtaagca cgattgctaa
     1801 tgttcccctc ttcataggcc tgctccaaac catcactttc aatactaaga atcaagagtt
     1861 tcaaagcagc ccaatcttct tgatcatcct ggttttcttg gcttaaaatg agattttcaa
     1921 tacgtccatg ataattgtca atagccgcat agaggggaag tttatttctg gtgtcttcca
     1981 actctttttc caactctagc gttacttcat tcaaaatggc gatatgcatg agataatcct
     2041 tgctttcttc ctcttcatca gaaagatgag gcaagaccaa gagacctgtt aaaaagctga
     2101 caagcgtcac acctgcaaca aggaaaagca agagaggata ctcctgttct agattacttg
     2161 gtatcaagag aatcgtagca atcgacaccg ttcccttaac acctgaaaag gtcaagagaa
     2221 acatgtcctt catatactta tttagctttt tcttgaggcg tcgggttcta taggcataat
     2281 agccatagat cataataaaa cgaatgacaa agaggacaaa ggtaagggcg atgagagata
     2341 gcaataaaag tagaggatta tagattggat tggtcaagat aggttctgct atcatttcca
     2401 actccatccc taaaatcaca aagacagaac cgttgagcat aaaggtcact gtatgccaga
     2461 ccgtctcggt caccgtatcc acttgggctt cgaggagcgt gattttcttg aagcgacttg
     2521 cctttaaaat tccagcaact acgacggcaa taatccctga aacatggact tcttctgcca
     2581 gaaagaaggt cactagaggc aaactcaatt ctaataaaag ttcactggca atatccgttg
     2641 cgcgcacact tagcaagaag gtatggagga agcggttggt catggctgtt aaaaatccaa
     2701 ttaaaaaacc gcctaggatt gaaaagatga gcgaactgct agcttgcccc agagaaaaag
     2761 ctccagttgt ccaagctgtc aaagctacct gaaaagccac caaaccagaa gcatcattca
     2821 agagtccttc gcccttaaga atattggaca cgcgcttagg aaagctaaaa cgctccgaaa
     2881 gagaggcaaa ggccaccaag tccgtaggac caagggctgc accaacagcc aagcaagctg
     2941 ccaagggaag gctgaaccaa agaagatggg ccaagccacc caaactcagg gtcgagataa
     3001 aaatcactgg aaatatgaga taaacaatga ttcgccagtg ttttaaaata gccgtaacat
     3061 ctgcttcttc agcctctcgg aaaagcaagg gtccgataac cagtgccaaa aacaactccg
     3121 tattaaggtg aaagtcagta ttgggtaaaa agagaccaat cacaattccc aaaagaatct
     3181 gcaccaaagg gagaggcaaa aagggcagga gcttattggt tgtacttgag acaatcaaaa
     3241 ccagtaaaaa taggatgagg taaatcagta attccacgca cgtcctcctt aatctttttt
     3301 acaacaggat tcaaatatct ccttctgctc tttgattttt tggtcaatct tggaacagtc
     3361 tttgtgctca atttttctct ggcaccgttc catttcaaga gcaactaatt ttttcttgat
     3421 tttaagcatt tttttgctca tatgcgcttg gtctagcacg cccaccgctc gttcgtggtg
     3481 ggttgattca acaaaattct ggcgcatggc atccagcttt tcgtgtaggt attgtttatc
     3541 catgtctgta tctctctaat ttttcaatca tcactaaaaa cggcgggttg ttgacttggt
     3601 ttaaagttcg gtaaatggca gctgtgtact cttgttggtt caactggatc acaaaatcca
     3661 agacagcatc tctctcgagg tcgcctcctt catgaccata gtaaatcata atagcaattc
     3721 gtccaccttt gacaagtaag ccacatagct tttctaatgc ctcaatcgtt gtctgcggtc
     3781 gggtgatgac agacttatca gctgacggca aatagcccaa attaaaaatc cctgccttag
     3841 cttttgtcac aaactggtcc agtgtctcat ggccttgcaa gattaactgg gcatttgtca
     3901 agccagcctg atgcaaacgc tcttgggtct tttccaaggc ttgcttctga atatcaaagg
     3961 catagacttg cttggctagc ttggctaaaa aaagcgtgtc atgaccattt cccatagtcg
     4021 catccactac gacatcctct tttgtcacga cctcagccaa aaaatcatgt gccatctcaa
     4081 gtggtctttt cattttcaaa ctcctgtttt acagccttgc atccttgaac acttccacga
     4141 cgtcgcatct ccatctcaat gctgttgaga gcttcccatt tattgaggct ccacataagc
     4201 ccaagcagca tatccctagg cgcatctcct gtaattcgat ggatgacgat atgtttggga
     4261 atgatttcca gttggtcaca gatgaccctg acatattcgt cctgactcat caattgtaaa
     4321 cgtccctcat ggtaatctcg ttgcatacga gtatttgtca taagatggag caaatgcagt
     4381 ttaatccctt gaatatcgtt attcgtgaca caacggcgga cattttcaac catcatctca
     4441 tgggtttcac caggcaaacc attgatcaaa tgggaaacaa tctcaatttt tggatacttt
     4501 ctcaaacgct tgaccgtttc cacctacaat tcataagaat gcgcacggtt aatcaggtca
     4561 gaggttgctt cataagtagt ttgcaagccc aattcaaccg tcacatgcat gcactccgat
     4621 aactcagcca aatattcgat ggtttcgtct ggtaaacagt ctgggcgcgt tccaatattg
     4681 atacctacca cacctggctc attgatagcc tgttcataac gctctcgaat aacttccacc
     4741 ttttcatggg tgttggtaaa attttgaaaa taaaccagat acttctgaac atccggccac
     4801 ttacggtgca taaagtcaat ttccttataa aattgctcac ggataggcgc atctggggct
     4861 acaatagcat ctccagaacc tgaaaccgta caaaaagtac agcctccatg agccacagtt
     4921 ccatcacgat tgggacagtc aaatcccgca tcaataggga ctttaaaagt cttttctcca
     4981 aagagttttc gataataatc attcaaggta ttataagatt tcatgacttt cattataaca
     5041 aaaatcaccc acaatctcaa aagcctgact ttcctataaa ttcctctgtt tctcgtttcc
     5101 attagccttt ttttatgata caatatgggt atgattttaa tgaaattagc atctatttta
     5161 ttattgatac tgaccttagt cgtctgcatt atcctaacca aactttttag attaaaaaaa
     5221 ctaggacgaa actttgcgga tttggctttt ccagtcttgg tatttgagta ttacttgatt
     5281 acagctaaaa cctttaccca taatttcctc cctagactgg ggctagccct ctcgatccta
     5341 gccattattc tcgtcttttt cttccttttg aaaaaacgca gcttttacta tcctaaattt
     5401 atcaaattct tctggcgtgc aggattctta ttaaccctta tcatgtatat agaaatgatt
     5461 gttgaattgt tcttaatgaa atagtcgaat ccctaagcat tttctaggga tttttgcttt
     5521 ctctgcaaaa tagtatagac aataacacta tacaatttta tacaaagaaa agagtctggg
     5581 acaatagtct cttatatcca aaaaggcaac ggatttgccg ttgctttttt gcatggttac
     5641 gatagtcttg gtaaaataga attgcccaat aaaccattta gaaaggctat cccatgcata
     5701 ttcactataa cacaaatcaa acaactttac cactagaaat cagttccttc ttaccacaag
     5761 atcatctcgt ttttactatt gaaaaagtgg tgaatacctt ggaggaacgt cacttctaca
     5821 cctcctatca tgcctttgat cgcccgtctt atcaccctaa aatgcttgta tctactcttc
     5881 tatttgccta ttcacaaggg attttctctg gtcgaaaaat tgaaaaatgg aagagttagt
     5941 gaccttagat tgtttgttta ttgacagaac taagattgaa gccaatgcca acaagtatag
     6001 ttttgtgtgg aagaaaacga cagagaaatt ctccgccaaa cttcaagaac agatacaggt
     6061 ctattttcaa gaagaaatca ctccccttct gattaaatat gccatgtttg ataagaaaca
     6121 aaagagaggg tataaagagt cagctaaaaa cttagcgaat tggcactata atgacaagga
     6181 ggatagctac acacatcctg atggctggta ttatcgtttt caccatacca aatatcagaa
     6241 aacacagaca gactttcaac aagaaatcaa ggtttactac gccgacgaac ctgaatcagc
     6301 ccctcaaaag ggactgtata tgaacgaacg ctatcaaaac ttgaaagcta aagaatgtca
     6361 ggcgctttta tctccccaag gtagacagat tttcgctcaa cgcaagattg atgtggaacc
     6421 tgtctttggg cagataaagg cttctttggg ttacaagaga tgtaatctga gagggaagcg
     6481 tcaagtgaga attgacatgg gattggtact tatggccaat aacctcctaa aatatagtaa
     6541 aatgaaataa gaacaggaca aatcgataag gacaatcaaa tcgatttcta acaatgtttt
     6601 agaagtaaaa gtgtactatt ctagtttcaa tctactatac aataagagaa tgactcaaaa
     6661 ttaaaaagct agagttccac aattggaaat ctctagcttt tttgtggttg agaactattt
     6721 tgtctcaggc tctttatctt ctatttagga caagagtttt tctttggtct ttaatgataa
     6781 agaaggtatc aaaatttcta gtcttctttt ttacctttag taactactaa tcctgcactc
     6841 aaacctagaa gagttaaacc tgctgctact gctgcttggc ttgccgcact acctgtactt
     6901 ggtaactggg ctttattagt ttgactagct tcacttgaat caattggttt tgtatctgct
     6961 ttttctgaca cttgtggttt tttagcttct tgagctactg gtttggttcc aaccaagacg
     7021 atgcggtctg tcggaacttc taccacttca cggagttttt cttccttact tccatcagga
     7081 ttaatcgctg taaagatacg ttctttacca gtttttcctt cttgttctac acgagtttca
     7141 cctagataca gtgttgaatc ttttttctca actgtcttgt atgccaaatc tttttcaaca
     7201 aattcgattt ttggaagatc ttcttgtaca gcagcaactg tcttctcaga aactggtttt
     7261 tccttagtca agtggatacg gtattccttg acttgttttc cactttctga aacgaggcga
     7321 acaagtactg gaaagctatc ttctccacta tctaccacag ttgaagctac ttgattgttt
     7381 tcttcaactg agacttttgg ccgttgacct ttataggtaa tttgatagtc ttgacgattt
     7441 tcagcgaaat cagcaagttc ttttccatct acaagaatct ttgattgagt gctttcttga
     7501 ggcaattcac ttggtgcaag gaaggtcatc tcaatcatcg caacaccgct cttatctgct
     7561 ttacgctcca tacgccatct catagctttg gctttgatag ctttaaatgt tacgttgatt
     7621 tcatcaccag ctgcaatgtc tttatccgca cgataaggaa cagcttccca attttctgga
     7681 ttgttgaatg gatggtctgc gtcgtaggct tggtagtttg aatagtaggt tggcacttca
     7741 aactctggac cgacatagcg ttctaaaacg agtttagatg gtgcatccgt accactatct
     7801 gcaaagaact gaacttttcc ttgtgcaaca gtccgttcta caatcttacc attttcacgg
     7861 aaaatcacac ccgctgatac ttctggatta gaagatggtg ttggtgacca gtttgtccaa
     7921 cgacgatttt ctgaatgatc tccgtcattg agatagtcaa cgcggtcatg agagtttttg
     7981 tcaatatcat tggttgctga agcaaaggcc tggttactgt tttcatcata gttagggtta
     8041 tctgaaagag tctcaccaag tttgtctgtc actcgtacag tgacctcagc aacaaggtta
     8101 ctaccaagga cacggcctcg aacagtaaat tgacctgctt ttgtcagatt ttccgctgga
     8161 acttcttccc attcaactgt caggtctttt gtttcgtagc cgtctttacc tgtgaagtaa
     8221 actggaacct tagtcggcaa ttcaagtgct tgacctactt gtagcaagcg agcttgttta
     8281 accgcagcaa ctggtttatg agaaagtaag ctcttatcct tagtgaagtg cagacggtat
     8341 tctcctaaga tgtcgccatt ttcagctttc gcgatgacac gaactggctc accttcacga
     8401 acgcttggaa cgacggtagc gagaccattg ttgctaacac ttgctgtgac tgccggaact
     8461 tttccatcta cagactcaag gtagtagtct gtcaaatcag ggttgaagtt tgctaagtct
     8521 ttgccgtcaa cttggattct tgtttgtcct tgcttggctg ccgcaacttg tttcgcaaag
     8581 atttgtacct ctgtgataga cgttccacgc ttgttatctg ctttaaccat gcgaatacga
     8641 acagcatagg tttcaacttt atcaaagcta aagtggttca tttctccagc cttgagttga
     8701 gcaggggctt ttagattagt aactggtttc cagttggcag aatcattaaa gacatggtcc
     8761 tcattaccaa caaaactagg gtttttagga gctgttggga cagtcttacc aacataatac
     8821 tcaatcacat aagacttcgg tacaccaact ccatggtctt catggaatcc gacacttaga
     8881 ttatcaacgg agcgtttgct caagatacct gaatctccaa acagaacacc gactgaagct
     8941 tctggattag tacgattcca gtttgtccaa cgattggctg gttggttatt gtaggaaatg
     9001 agcttgtcat taacatttga aactgggtcg cttggatttg agtctgaagc aaaggcaagt
     9061 ggcaattctg aaccggtcca ttggtcagaa atgtttgcac cttgctcagt ttgagcagat
     9121 acgcgaacat gaagtttagt tgttaattgc gtaccttcta agcgaccatt aactgtaaag
     9181 acaccttcct tagcgtattg ctctggacga atcgcatccc atgcaacctt agctgatgaa
     9241 acgtgaccat ttgaatcata tgtccgaaca ctttctggta attgtggtgc ttctgcgatt
     9301 ggagttgtca cactgacttc ttcaactgaa acgatacctt ctacagagac ttttgcacgc
     9361 gcttcaaggt caattccttc aactttacct agtacttcaa atgtttgata ggagtctagt
     9421 ttttctttcg gaatagcttg ccaagtgact ttatgagttt tagggaaacc tttgtcatac
     9481 tcaactgtta ctgttgctgg aagacttggt tcctgatgca aatctgtcac tacatttaca
     9541 ggacggatgg attgcgcaat cttcttctca gtattggctt ggatagtgag ttcaacttgg
     9601 tctttagctc cctcatattc agcgttcaga gtgactgctc ctggcttatg caactcaagc
     9661 attcctttac gaattgcgac ttccccttca ccacttgtag agaaggttac tttatcagct
     9721 ggtaatacag cttgcgttcc atcttgatag tgagctcgaa ccgacaattt gacagtttgg
     9781 tcttctttga gactgtcagc tttttccact tgcaagctca agtgagcaat ttttggcgct
     9841 tcttcaagga attgaattgc ataggtttga agagggccac catctttagg ctgaataaag
     9901 atgctcgcac gcatgccgtt tgctgcgctt gcttgaagaa ctgtcacatc agcattttca
     9961 gcacttgctg tgacttctgg caattgggca ccataagata gagtacgata ttgcattggt
    10021 tgtcgactag taagacctgt tactgcttca cctccaacag taacagttgg cactacaggt
    10081 gctgcagcat ttccttcttc taccacaagg gttgcatgaa tagtttctcc agctaactga
    10141 ccagtcattt gaatacgtga tccagctact gaaagtttgg ctttatctac ttccgtaatc
    10201 tcccagctat ctacttcgta ctcttgtaca cttccatcag tcaaaacata agaaacagat
    10261 ttgtctacag aattcaagtc agtatttgga gcaatacgtt taactgttgg taattctttc
    10321 gcaattgcta gaaccttgac atgagcctct acctcacgtc catttgctgt acctttaaca
    10381 gtcacaactc ctgcttgact aacatcgact tgagaccagg ttacaggacg ttctgcacgg
    10441 ctaccatcac tgtatacaaa cggaacagtg gtaggcattt caggtgcctc tccaataatg
    10501 gtctgtactt ttggcacttc tgtccccaaa acagtcttct cttgtccttc tttcttacca
    10561 gtaaagacag tgacttggtt cgatttcaag agatcagagt gggcagtaag ggtgaatttc
    10621 cctgcttgtt cagttgattt gacaatggca acacctttac cattaaatgc tttacgaatc
    10681 caagaaccat ctgcttgcgc cttatagcgt tcacggctgg cttgttctcc gttatctaca
    10741 ccgaccagtt gaccttgacc atgcaattgg aagcgaacca gattattagc agttggaacc
    10801 acattcccct ggctgtcaac aatttcatag tagatgtaag tcaagtcttt tccatctgct
    10861 gcaatcgcat gatcttcctt aataagacga actgccgctg gcttaccagc agtcgtaatc
    10921 ttatctcgag caatttcctt gccagattca tcacgagcaa ttgcttccaa ggtacctggt
    10981 tgataggcaa ctttccattc aagataaagt tcattagcat ttgcaccttc ttggtaagtc
    11041 cgcccatcgc tggtttgttt tttattgaaa gtcttaagac caagagattt tccattcaag
    11101 aacaattcta cactagaagc attcgaataa gcacgaactg gaatcttacc ttctgagtca
    11161 gctactttgg atgctaattc tttgttttcc cagttccagt gaggaagaag gtgtaccatc
    11221 ggtttcttct taacagaaac ccattggctt tggtagagat agaagtcatg ttttggaatg
    11281 ccggctgtat ctacgatacc aaagtaagag ctcttaacgg gagtttgatt ttggttgtgc
    11341 catggtgtag gttcaccaat atagtccgta cctgtccaga taaactgtcc agcatagcca
    11401 gcgttgtcac ggtcaaaagt ccatgaagca gttgctgttt tcccccaacc cacacgatca
    11461 tttccataat ctgactgttc ataattacgc tcaggtccat tgctatgttt caattcacgt
    11521 tcagggcgat agtaacttcc tcgtgtacgg gttgctgatg atgtttctga accgtaaatc
    11581 aaccattttg gatgcttagc tctaagggct ttgtaattat cttcagaata gttaaatcca
    11641 acagcatcga gttcatcagc aattttctca tgccctccgc taccattacc gaaacggaat
    11701 ttatctgctc ccatggtaac atagcgagtc ttatcaacat ccttgataac cttgaccaaa
    11761 cgtttaacag ttgctaaaga gtgggcatca ccattagctt cacctatttc attaccaatt
    11821 gaccacatga agatagcagg gttgtttttg cctctttcga ccatggtacg taggtcaaaa
    11881 tcagaccatt tttcaccttt tcgagcttct gggtgagtgg catctttttc aaagaaacgt
    11941 ccatagtcat aaggtttctt gccaccatac caagtatcaa aggcctcttc ctgaacgagt
    12001 aaacctagtt ctgctgcgat ttgcaaggtt tgctcactag cagggttgtg ggttgtacgg
    12061 atggagttaa ctcccatctc cttcatttgt ttgagacggc gatattctgc tttatagttt
    12121 tcttctgctc caagcgcccc atggtcgtgg tgcaaggata ctccatggaa tttaatacgt
    12181 tcaccattca aagagaaacc ttcatttgga gtccagtgat agtaacggta accaaacaaa
    12241 tccttcttag catcaaccaa ttgaccgtca cggtaaacac gcgtaatcaa ttcgtacaag
    12301 gcaggtttgt catttaaaac ggtccagagt tttggtcttt caacttctaa aatcgcatct
    12361 aggcttgttg attcatgtgc ttttaaggta cgactcgctg tacgaactaa gcctgttaca
    12421 gcatgaccac ctcgttcaac gatttgatat tcggctacaa gttcatggtc tttgtcgtcc
    12481 gtattgacga ttttgctggt cacatgagtt tcaaccttgc catgttgttg ttcttcaagt
    12541 tttggtgtta aaatagttgt cccatttttc tcaacatgca ccttatctgt cacttgtaaa
    12601 gtcacatcac gatagatacc acttcctgaa taccaacggc tacttggctg tttgttgact
    12661 gcatggacag caatcacatt ctcacgacca tctttgtaaa ggtatttggt gatatcgtat
    12721 gagaactggt tataaccatt tggataatgc cccactaact gaccattgac ataaacttga
    12781 gaatccatgt agacgccatc aaaagtaagg cgaacatttt tcttgaggtc tttttcatct
    12841 agtttgaaag tcttgcgata ccaagcttcc ccaccgttga gctgtccacc ttcattttgt
    12901 gcaggagatt catgatcgaa atcgttaaag atactccagt catacggtaa atctaatttt
    12961 ttccacgtag atacgtctgc atcaggttta atggcttcct tagaatttgc attgagttta
    13021 aagtaccaat tttgattaaa atccactttc ctgttttcaa tcatttgatt cacttcttca
    13081 tttgttacag ctttagcatc ttccttgagc ggtttttctt gacttgaagc ttgtgattct
    13141 atccttggag ctttttcttc cggtttagca gacacttttt cctcttttgg agttacggct
    13201 tcatcttctt tcttctcaga tgcaatagcc tcagttgaac taggttcact ttgttctgtc
    13261 ctttcaacta catttttagt ttccaaagct ttatcagcct tttcttctac tatcattttt
    13321 tcctctttag gtttctcagc agtatgagta ataagtgttt catccgcata aactacagat
    13381 tctccagcta tatttcctcc taatagaact gcacaagtcc caatcattac tgagcaagct
    13441 cccacagcaa acttacgaat gctataaact cttttccgat tccaatggcc tttccccata
    13501 aaaccctcct tatattatat ttagtgcagt tagctactgc caaagcccaa gtggtataca
    13561 tggtatgaca acctagtttc aacaatttac actctgcgaa aatccaattc aaacttcgtc
    13621 agtgtcgcct tgccgtatgt atgattactg acttcgtcag tttcatctac aacctcaaaa
    13681 ccatgttttg agctgacttc gtcagtctta tctacaacct caaaactgtg tttttagcaa
    13741 cctgcggcta gcttcctagt ttgctctttg attttcattg agtttatatt ttataggagc
    13801 gcattatttt gcttttgctg cgtactcttc gttacgtttg atcatttgtt ttctgtacca
    13861 agcaaagata ccgatataga atacaaggaa ggctactgca ccaaggattg ctttgatatc
    13921 accagttgta gtgttaccaa ttgtccaacc aagaagtttt tcgattggtc cttcaagagt
    13981 agagtgagta atcaattgag tttggctcac accttctggg aaggcaccta cacctttagc
    14041 aagttctgtt gcaaatggtg caataagtgt acctgaaaga aggaagagtg gcaacaagag
    14101 tgttccgaag ataatcatac ggagcaattt accacgagtt acaaccaaga gagctggagt
    14161 aacacccata gcgatgatac ctgcaagtgg caagatacca tttccaactt ttgaaagaag
    14221 cactgcttca atcaacatga ttggtgcaag tacgttggca caagcccaga tttcagcacg
    14281 accagcgatg aatggccagt caagaccgat attgaattta cgtccttgaa gacgtttagt
    14341 agcaacgttt gtaatacctt gtgatagtgg ttctacggct gcgatgaacc atgaaccgat
    14401 aagtgagaag agttccaaag atacaccggc agtcaaacca agagacaacc atcctttgat
    14461 aacaagacgc catttatctg catctgcaac acctgcaatt ggatgtggag ttcccataat
    14521 accgataacg ataccaagga tgaaaccgat gaagaattta gatccccaga aaccgatttt
    14581 cttgttcaat ttagcagcat caaagtcata tttatcaagg cctgggaaga atttttcaaa
    14641 aatcttatcc aaaaccatga taactgggtt catcatgtag ttcatgtgag ttgatgtcat
    14701 tggtgatgaa cttggggcgt taagaaggtc atcaaatgta ggtttcatca agtcagagtt
    14761 gataattttc aacacaccga caagaacgat agctgctgta gcaatcaaga gtgaaacccc
    14821 ttgactcaca ccattgttat cagcatacca tttaatcaag agacctgtga tagacaagtg
    14881 ccagatatca aagatatcga catcaagtgt atcggttttc ttcatagcaa gcatcactac
    14941 gttgacaatc aacatgatga gcaagaagta aagtgtccaa gcagaacccc aagtgattgt
    15001 tgcaagtggt gcccaaccaa cgtcggtaat actcaattgg ataccagtgt tttcaacgaa
    15061 ttttgcaagt gatgctgaga aagcaccatt tagcataccg atgatagcac cgatacctgt
    15121 aagagcgatg gcaagtttga taccaccttc aagcgctttg gagaatttca ctccaaaaag
    15181 taaagccaat actgtcaaaa tgattaacat gatgacaggt ccacccattt ctaagatggg
    15241 attgaaaacc tttccgatta ggtcaaagat tgcatccata acagttcctc ccttttttat
    15301 gttatatgaa tgttaacaaa ttagaattag cttaatccgt gttctttaat agctgcttca
    15361 atattgtcaa atactgga
//
LOCUS       DAVMEF010000036        14995 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMEF010000036 DAVMEF010000000
VERSION     DAVMEF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.036"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..572
                     /gene="murN"
                     /locus_tag="VYQ30_000792"
     CDS             <1..572
                     /gene="murN"
                     /locus_tag="VYQ30_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1965942.1"
                     /translation="FYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLS
                     KNPHSEKKQNQLREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYL
                     FSGSYTEFNKFYAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGY
                     IVRKAGTFRYHPSPLKYKAIQLLKKIVGR"
     gene            651..1352
                     /locus_tag="VYQ30_000793"
     CDS             651..1352
                     /locus_tag="VYQ30_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1965943.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            1392..3236
                     /gene="uvrC"
                     /locus_tag="VYQ30_000794"
     CDS             1392..3236
                     /gene="uvrC"
                     /locus_tag="VYQ30_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1965944.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            3226..4059
                     /locus_tag="VYQ30_000795"
     CDS             3226..4059
                     /locus_tag="VYQ30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1965945.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDFFDF
                     L"
     gene            complement(4241..5041)
                     /locus_tag="VYQ30_000796"
     CDS             complement(4241..5041)
                     /locus_tag="VYQ30_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1965946.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            5212..5817
                     /locus_tag="VYQ30_000797"
     CDS             5212..5817
                     /locus_tag="VYQ30_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1965947.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            5830..7230
                     /gene="pepV"
                     /locus_tag="VYQ30_000798"
     CDS             5830..7230
                     /gene="pepV"
                     /locus_tag="VYQ30_000798"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1965948.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            7284..7862
                     /locus_tag="VYQ30_000799"
     CDS             7284..7862
                     /locus_tag="VYQ30_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1965949.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            8022..8174
                     /locus_tag="VYQ30_000800"
     CDS             8022..8174
                     /locus_tag="VYQ30_000800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1965950.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            8217..8309
                     /locus_tag="VYQ30_000801"
     CDS             8217..8309
                     /locus_tag="VYQ30_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1965951.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            8480..9805
                     /gene="brnQ"
                     /locus_tag="VYQ30_000802"
     CDS             8480..9805
                     /gene="brnQ"
                     /locus_tag="VYQ30_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1965952.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            10009..11046
                     /locus_tag="VYQ30_000803"
     CDS             10009..11046
                     /locus_tag="VYQ30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1965953.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            11048..11518
                     /locus_tag="VYQ30_000804"
     CDS             11048..11518
                     /locus_tag="VYQ30_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1965954.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            11521..12237
                     /locus_tag="VYQ30_000805"
     CDS             11521..12237
                     /locus_tag="VYQ30_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1965955.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(12277..12423)
                     /locus_tag="VYQ30_000806"
     CDS             complement(12277..12423)
                     /locus_tag="VYQ30_000806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965956.1"
                     /translation="MYSMKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTD
                     AVSR"
     gene            12546..12971
                     /gene="rplK"
                     /locus_tag="VYQ30_000807"
     CDS             12546..12971
                     /gene="rplK"
                     /locus_tag="VYQ30_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1965957.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            13179..13868
                     /gene="rplA"
                     /locus_tag="VYQ30_000808"
     CDS             13179..13868
                     /gene="rplA"
                     /locus_tag="VYQ30_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1965958.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(14322..14402)
                     /locus_tag="VYQ30_000809"
                     /pseudo
     CDS             complement(14322..14402)
                     /locus_tag="VYQ30_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            14410..14772
                     /locus_tag="VYQ30_000810"
     CDS             14410..14772
                     /locus_tag="VYQ30_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965959.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKIFVTVIGYFEINIDENITDILYVNGTAILYLYLRSIVSIVSAIDSSEAMLL
                     PIINVLELLDKSQPFEEE"
BASE COUNT         4446 a         2899 c         3381 g         4269 t
ORIGIN      
        1 atttttatga tacttttgga gaacaagcgg agtttctcat agcaagcttg aatttttcgg
       61 agtatatgag caaattgcaa ggtgaacaaa gtaaactaga agaaaaattg gacaagttgc
      121 gacttgattt gagtaaaaat cctcattctg agaaaaaaca aaatcaactg agagaatatt
      181 ctagtcaatt tgaaacgttt gaagttcgaa aagcagaagc gcgagacttg attgaaaaat
      241 atggagaaga agatattgtt ttagctggta gtttatttgt ttatatgcct caggaaacga
      301 cttatctctt tagtggttcc tacactgagt ttaataagtt ctatgcccct gcactgcttc
      361 aaaaatatgt tatgttggaa agcataaaac gtggaatacc taaatacaac ttcctaggca
      421 ttcaagggat ttttgatgga agtgatggtg ttttgcgttt taaacagaat tttaatggct
      481 atattgtacg caaagcgggt actttccgtt accatccatc gcctttaaaa tacaaagcta
      541 tccagttact caaaaaaata gtaggacgtt aagatgaaaa agtcagtatt tagatttctt
      601 ttagcttctt ttagtaaaat aattcttatt tgctagaaag gtggagagac atgcgctggc
      661 tttttcgttt gataggggct ttcttttctt ttgtgtggcg tttgttttgg cgtctggttt
      721 ggatagttgt gctcttatgt gtgcttgctt tcggacttct ctggtatctg aacggggatt
      781 ttcaaggagc gctaaagcaa gcagaacggt cagtaaaaat tggtcaacaa agtattgacc
      841 aatgggagaa aacagggcaa ctgcctaagt taagccagac agatagtcac cagcattctg
      901 aaggaaggtg ggcacaggcc tctgctcgta tttacctgga tccgcagatg gattcacgct
      961 ttcaagaggc ttatttagaa gcaatccaga actggaatca aacgggtgct tttaactttg
     1021 aactcgtgac tgagtctagt aaggcggata ttacggctac ggagatgaac gacggaggca
     1081 ctcctgtggc aggagaggcg gaaagtcaaa ctaatctctt aacagggcaa ttcttgtccg
     1141 taacggtgcg gttgaatcat tattatttgt ccaatccata ctatggctac tcctatgaac
     1201 gccttgtcca tacggcagaa catgagttag gtcatgcgat tggcttggac catacagatg
     1261 agaagtctgt catgcaacca gcaggttcct tttatggtat ccaggaagag gatgttgcaa
     1321 acctccgaaa aatatatgag actagtgagt agggtactat ctttccctac tttttttgct
     1381 ataatggaac tatgaacaac ttgattaaat caaaactaga gctcttgccg accagccctg
     1441 gttgctacat tcataaggat aaaaatggca ccattatcta tgtaggaaag gctaaaaatc
     1501 tgcgtaatcg agtacggtcc tattttcgtg gaagtcatga taccaagaca gaggctctgg
     1561 tgtctgaaat tgtggatttt gaatttattg ttacggagtc taatattgag gcacttctcc
     1621 tagaaatcaa cctgatcaag gaaaacaagc ccaagtacaa tatcatgctc aaggatgaca
     1681 agtcctatcc tttcatcaaa atcaccaatg agcgctatcc acgcttgatt atcactcgtc
     1741 aggtcaaaaa ggacggaggt ctttattttg gaccctatcc cgatgtgggg gcagccaatg
     1801 aaatcaagcg gttgctggat cggatattcc cttttcgtaa gtgtaccaac ccgccctcta
     1861 aggtctgttt ttattaccat atcggtcaat gcatggccca caccatctgt aagaaggatg
     1921 agacttattt caagtctatg gcccaggagg tttctgattt tctgaaaggg caggataaca
     1981 aaatcattga tgagctaaag ggcaaaatgg cagcggcagc ccagactatg gagtttgaac
     2041 gtgcggcgga ataccgtgac ctgattcagg ctattggaac gcttcgaacc aagcaacggg
     2101 tcatggctaa ggatctccaa aatcgcgatg tctttggcta ctatgtggat aagggctgga
     2161 tgtgtgtgca ggttttcttt gtccgtcagg gtaagctcat cgagcgcgat gtcaatctct
     2221 tcccctactt caatgatcca gatgaggatt ttttgaccta tgtaggacaa ttctatcaag
     2281 aaaaatctca cctagttccc aatgaggtac tgattccgca ggatattgac gaagaggctg
     2341 tcaaggcttt ggtggatacc aagattctca aaccccaacg tggagagaaa aaacagctgg
     2401 tcaatctagc cataaaaaat gcacgtgtta gtctggagca gaaatttaac ctgctagaga
     2461 aatctgtcga gaagacccaa ggagctattg aaaatctagg acgtttgctc caaatcccga
     2521 ccccagtacg tatcgagtcc ttcgataact ctaatatcat gggaactagc cctgtttcgg
     2581 ctatggtggt ctttgtcaac ggtaaaccga gtaagaagga ttaccgtaag tacaagataa
     2641 aaacggttgt tggaccagac gactatgcca gcatgagaga ggtcattcgc agacgctatg
     2701 gtcgagtaca gcgtgaggct ttgactcctc cagatttgat tgtgattgat ggggggcaag
     2761 gtcaagtcaa tatcgctaag caggttatcc aagaggaact gggcttggat attccaattg
     2821 ctgggctgca aaagaatgat aagcaccaaa cccatgaatt gctctttgga gatccgcttg
     2881 aggtggtgga cttgtctcgc aattctcagg aatttttcct cctccaacgc atccaagatg
     2941 aggtgcaccg ctttgccatt accttccacc gccaactgcg ctccaaaaat tctttctcat
     3001 ctcaattgga tgggattgac ggtctgggac ctaaacgcaa gcagaatctt atgaagcatt
     3061 tcaagtcttt gaccaaaatc aaggaagcca gtgtggatga gattgtcgaa gttggggtac
     3121 ctagagtcgt tgcagaggct gtgcaaagaa agttgaaccc gcagggagaa gccttgcctc
     3181 aagtagcaga agaaagagta gattaccaaa cggaaggaaa ccacaatgaa ccataaaatc
     3241 gcaattttat cagatgttca tggcaatgcg acggcgctag aagcagtgat tgcagatgct
     3301 aaaaatcaag gggccagtga atattggctt ctgggagata tttttcttcc tggtccaggc
     3361 gcaaatgact tagtcgccct gctaaaggac cttcctatca cagcaagtgt tcgaggcaat
     3421 tgggatgatc gtgtccttga ggctttagat gggcaatatg gcttagaaga cccacaggaa
     3481 gttcagctct tgcgtatgac ccagtttttg atggagcgaa tggatcctgc aacgattgtc
     3541 tggctacgaa gcttgccttt gctggaaaag aaagaaattg acggattgcg cttttctatc
     3601 tctcataatt tacctgacaa aaactatggt ggtgacttgc tagttgagaa tgatacagag
     3661 aaatttgacc aactactaga tgcggaaacg gacgtggcag tttatggtca tgttcacaag
     3721 cagttgcttc gttatggaag tcaagggcaa caaatcatca atccagggtc gattggcatg
     3781 ccctatttta attgggaggc gttaaaaaat caccgtgccc agtatgccgt gatagaagtt
     3841 gaagatggag aattactcaa tatccaattt cgtaaagttg cttatgatta cgaagctgag
     3901 ttagaattgg ccaagtccaa ggggcttccc tttatcgaaa tgtatgaaga actgcgtcgt
     3961 gaagataact atcaggggca caatctggaa ttattagcca gcttaataga aaagcatggg
     4021 tatgtagagg atgtgaagga tttttttgat tttttgtaag agtttcctaa aatagccaat
     4081 gcaaactaaa aaagcgattg gctggtccaa tcgcttttag tatatcttat actcaatgaa
     4141 aatcaaagag caaactagga agctagccgt aggttgctca aagcacagct ttgaggttgc
     4201 agataaagct gacgtggttt gaagagattt tcgaagagtg ttattgtaac tgagattgat
     4261 ctgggaggta agaaccacct agataggtat tgctgagttt ttcaagggtt ccgtcttgat
     4321 agagttcttt gagcgcttta tcaaattgct ctttaaactc tttttggtcg cttgagaaaa
     4381 cgatatagtt gctggggcta tctgcagaag gtaaatcaac gactgagagg tctaaaccac
     4441 ggtccttgat aatcttttga acggatacct tgtcaaaaac taggaaatca aactctccgt
     4501 tagcaaggtc taggattcgt ttaccaatat cctcaccaga aaaattaatt gtagcgggat
     4561 tatcagtgtg tttctgattc cagttattga tgaattgagc gttagaagtt ccggtatcct
     4621 cttgtgttgt tttaccagcg atctggtcaa gagaagtcaa aggatttttc ttgttgctga
     4681 caaggacgag gggattgttg gaaattggaa gcgagtaaag gtatttttca gcacgctctt
     4741 ttgtgtaact caagttattg gccgcagcct gatagtgacc agaatcaagt cctgggaaga
     4801 tgctctccca ggcggttctt tggaattgaa tctcgtagtc gctgagtttt tcatctactg
     4861 cctttaaaac ttcgatatcg aagcctgtca gattgccctt gtcttcgtag tcaaatggtg
     4921 gcacgtcgcc agctgtagca aggacgattg tcttttgagc gctagtctct ttgggtgtag
     4981 cttgattccc acaggcaacc aaaaatggta ggatagctag taataggcta aattttttca
     5041 tactgtctcc attcaaatgt aaagtacttg ctagtttatc agaaactttc ctgataaacc
     5101 aatatatatc ttttatgtct gtgatagaaa aacttaatca tgaaaaatcc ctgcaagctc
     5161 tttcaaatta tggtagaatg gaagcagtag aattagaaaa ggaaatcgat tatgaaattt
     5221 cttgaattaa ataaaaaacg tcatgcgact aagcatttta ctgataagcc tgttgatccc
     5281 aaagatgtgc gtacggctat cgaaattgca accttggcgc caagcgccca caacagccag
     5341 ccttggaaat ttgtggtggt acgtgagaaa aatgctgaac tggcaaagtt agcttatggt
     5401 tccaattttg aacaggtatc atcagcgcct gtaaccattg ccttgtttac agatacggac
     5461 ttagccaaac gtgctcgtaa gattgcccgt gttggtggtg ctaataactt ttctgaagag
     5521 caacttcaat attttatgaa aaatctgcca gctgagtttg cccgttacag tgagcaacaa
     5581 gtcagcgact acctagctct caatgcaggt ttggttgcca tgaacttggt tcttgcattg
     5641 acagaccaag gaattggttc taacattatt cttggttttg acaaatcaaa agttaatgaa
     5701 gttttggaaa tcgaagaccg tttccgccca gaactcttga tcacagtggg ttatacagac
     5761 gaaaaattgg aaccaagcta ccgcttgcca gtagatgaaa tcatcgagaa aagatagaaa
     5821 gaagaaaaaa tgacagcaat tgattttaca gcagaagtag aaaaacgcaa agaagacctc
     5881 ttggctgact tgtttagcct tttggaaatc aattcagaac gtgatgacag caaggctgat
     5941 gcccagcatc catttgggcc tggtccagta aaagccttgg agaaattcct tgaaatcgca
     6001 gaccgcgatg gctacccaac taagaatgtt gataactatg caggacattt tgagtttggt
     6061 gatggagaag aagttctcgg aatctttgcc catatggatg tggtgcctgc tggtagcggt
     6121 tgggacacag acccttacac accaactatc aaagatggtc gcctttatgc gcgcggggct
     6181 tcggacgata agggtcctac aacagcttgt tactatggtt tgaaaatcat caaagaattg
     6241 ggtcttccaa cttctaagaa agttcgcttc atcgttggaa cagacgaaga atcaggctgg
     6301 gcagacatgg actactactt tgagcacgta ggacttgcca aaccagattt cggtttctca
     6361 ccagatgctg aatttccaat catcaatggt gaaaaaggaa atatcacgga atacctccac
     6421 tttgcaggag aaaatacagg tgttgcccgt cttcacagct ttacaggtgg tttacgtgaa
     6481 aatatggtac cagaatcagc aacagcagtc gtttcaggtg acttggctga cttgcaagct
     6541 aaactagatg cctttgttgc agaacacaaa cttagaggag aactccaaga agaagctggc
     6601 aaatacaagg tgacgatcat tggtaaatca gcccacggtg ctatgcctgc ttcaggtgtc
     6661 aatggcgcaa cttaccttgc cctcttcctc agccagtttg gctttgctgg tccagccaaa
     6721 gactaccttg acatcgcagg taaaattctc ttgaacgatc atgagggtga aaatctcaag
     6781 attgctcatg tggatgaaaa gatgggtgct ctttctatga atgccggcgt cttccgcttc
     6841 gatgaaacaa gtgctgataa taccattgcc ctcaacatcc gctatccaaa aggaacaagt
     6901 ccagaacaaa tcaagtcaat ccttgaaaac ttgccagttg tttctgttag cctgtctgaa
     6961 cacggtcaca cgcctcacta tgtgccaatg gaagatccac ttgtgcaaac cttgttgaat
     7021 atctatgaaa aacaaactgg ccttaaaggt catgaacaag tcatcggtgg tggaaccttt
     7081 ggtcgcttgc tagaacgcgg agttgcctac ggtgctatgt tcccagactc gattgatacc
     7141 atgcaccaag ccaatgaatt tatcgccttg gatgatcttt tccgagcagc agcaatttat
     7201 gccgaagcta tttacgaatt gatcaaataa aacgatagaa gtctgagatc ttatgcttgg
     7261 acttcttttt ggagggaaag tagatgtctc aaatcgaaag aatcaaacag gctatcatgg
     7321 cggattcaca gaatgccagc tatacagagc gtggcattga gcctctcttt gcagcgccaa
     7381 aaactgctcg catcaatatc atcggtcagg ctccgggact taaaactcaa gaagcaggcc
     7441 tttactggaa agataaaagt ggtgaccgct tgcgggactg gctaggtgtg gatgaagata
     7501 ccttttacaa ttcaggttat tttgctgttt tgcctatgga tttctacttt ccaggacatg
     7561 gcaagtcggg tgatcttccg cctcgtacag gttttgcaga aaaatggcat ccgcagctct
     7621 tacaggaatt gcctgatatt cagttaaccc tcttgattgg gcaatatgcc caagcctact
     7681 atttacagga gaaaatcagt gggaaggtaa cggagagggt gaaacactat aaagactatc
     7741 tgccagccta ttttccgcta gttcacccat caccacgaaa tcaaatctgg atggccaaaa
     7801 atccttggtt tgaggcagaa gtagtgccag atttgaaaaa aagaattaaa accattttat
     7861 agtcaatgaa aatcaaagag caaactagga agctagtcgt aggctgctca aagtacagct
     7921 ttgaagttgc agataaaact gacgaagtcg gtaacatacg cacggtaagg cgacgctgac
     7981 gtggtttgaa gagattttcg aagagtatta gaagaaaaag aatgaaagaa atagcctttg
     8041 acgcatttta ccagctttac caaaacgacc agctttcttt agtggatgtg agagaagtgg
     8101 atgagtttgc agctcttcat ttagaaggtg cccacaacct accgcttagt caattggctg
     8161 atagttatga ttaattggac aaagatcgct tgcattatat tatttgcaaa tctggaatga
     8221 gatcggcgcg tgcttgccaa ttcctattag aacaaggtta taatgttatc aatgtccagg
     8281 gtggcatgtt agcctttgaa gaactttaaa attttgcatt tctcctactt ggtgtggact
     8341 gggtaggaga gttttatttt tagataattc ttatttttaa gaaaattgaa aacatttaat
     8401 atttgcctcg tgatgctttt ttcagactcc taatcgtggt atactaggtc agtattttat
     8461 aaatatgaag gagattttta tggctaaaaa aggtacccta acaggtttgc tcctgtttgg
     8521 aatatttttt ggtgcgggga acttgatttt tccgccttct ctaggtgctc tatctggaga
     8581 acattttctt cctgccatcg caggttttgt cttttcaggc gttggtatcg ccgtcttgac
     8641 ccttattatt ggaacgctaa atcctaaagg gtatatctac gagatttcaa cgaagatagc
     8701 gccttggttt gcgactcttt acctctcagt tctttacttg tcaatcggtc cattctttgc
     8761 taccccacgt actgctacaa cagcttacga agtagggatt agcccccttt tgtcggatgc
     8821 aaataaagga cttggcttga ttgtatttac ggttctgtat tttgcggcag cctatttgat
     8881 ttcgcttaat ccatcaaaaa tcttagaccg cattggacgt attttaacgc cagtctttgc
     8941 aattttgatt gttatcttgg tcgttctggg agctatcaaa tatggtggaa caagtcctca
     9001 agctgcttca gctgcttatc aagcttctgc ctttggtaca ggtttcctag aaggttacaa
     9061 taccttggac gcccttgcct cagtggcctt tagcgtaatc gcagttcaaa ccttgaaaca
     9121 acttggattt tcaagtaaga aagaatacat ttcaactatt tgggttgttg gtatcgttgt
     9181 tgcccttgcc ttcagcgctc tttacatcgg tttaggtttt cttggaaatc atttcccagt
     9241 accagctgaa gcgatgaagg gtggaacacc aggtgtttac atcttgtcac aagccactca
     9301 agaaatcttt ggctcaacag ctcaactctt ccttgcagct atggttaccg taacctgctt
     9361 cacaacgact gttggtttga ttgtgtcaac agctgagttc tttaatgagc gcttcccaca
     9421 aatcagctac aaggtttatg cgacagcctt taccttgatt ggatttgcta ttgccaattt
     9481 gggtcttgat gcgattatca agtactcaat tccagtactg gttatcttgt acccaatcac
     9541 gattgctatc gttatgattg tcattgtcaa caaatttgtt gctctttcaa aaccaggtat
     9601 gcagttgaca attgctgtgg ttacagttat tgccattgca agcgtactag gaagctcgtt
     9661 taaggttgag tttcttgcaa atcttgttag cgttcttcct tttgccaagg catctctccc
     9721 atggttggtg ccagccattg ttggaatctt gctctcattg gttctaccaa acaagcaaga
     9781 aagcgatgtt tttgaaatgg aataatcact aatactcttc gaaaatcaaa ttcaaaccgc
     9841 gtcaacgtcg ccttgtcgta ctcaagtaca gcctgcggct agtttcctag tttgctcttt
     9901 gattttcatt gagtataaaa tcacttttgt agtcaagtct acaggagtga ttttcttttt
     9961 ttatccgatg ataaatgtgt tataataggt agcaaaagag gtgaagaaat gaatcaaaca
    10021 gtagaatata tcaaagaact gacagccatt gcgtcgccaa caggctttac tcgtgagatt
    10081 gcggactatt tagtcaagac tctagaaggt tttggttacc agccggttcg cacatccaag
    10141 ggcggtgtca atgtaactat taaaggtcaa aatgatgagc aacatcgcta tgtgactgcc
    10201 catgtagata cgcttggtgc tattgtccgt gctgtcaaac cagacggccg tctcaaaatg
    10261 gaccgtatcg gtggctttcc ttggaacatg attgaaggag aaaactgtac cattcatgtg
    10321 gctagcacag gtgaaaaagt atcaggaacc atcctcatcc accaaacttc ttgccatgtc
    10381 tataaggatg caggaactgc agaacgcacg caagacaata tggaagtgcg tttggacgcc
    10441 aaagtaacta gtgaaaaaga aactcgtgct cttggcattg aggtcggtga ttttatcagt
    10501 tttgacccac gaactgtcgt gacagagaca ggttttatca agtctcgcca tttggatgac
    10561 aaggtcagtg cggcgatttt gctcaatctc cttcgcattt ataaggaaga gaagattgaa
    10621 ttgcccgtaa caactcattt tgctttttca gtctttgaag aagtgggaca cggtgcaaac
    10681 tctaacattc ctgctcaggt agtagaatat ctggctgtgg atatgggagc catgggagat
    10741 gaccagcaaa cagacgaata tacagtgtct atctgtgtca aggatgcttc tggaccttat
    10801 cactatgact tccgtcaaca tttggtggct ttggcgaaag agcaagatat tccatttaag
    10861 ctggatatct atccatttta tggttcggac gcttcagcgg ctatgtctgc aggggcagaa
    10921 gtcaaacacg cccttctcgg tgctggtata gagtctagcc attcctatga gcgtacccat
    10981 attgactcgg tgatcgcaac agaacgaatg gtcgatgctt atcttaagag cacgttggtg
    11041 gactaatatg tgccttattt gtcagagaat tgacctcatc aagaaggaag aaaatcctta
    11101 ctttgtcaaa gagttggaaa caggctatct tgtggttgga gaccaccagt attttgaagg
    11161 ctatagtctc tttctagcca aggagcatgt cagcgaattg caccatttga aaaaggagac
    11221 aagactccgt tttctagaag aaatgagttt agtccaagag gcagttgcca aggcctttgc
    11281 tgctgagaaa atgaatatcg aactgctagg aaatggcgat gctcatcttc attggcatct
    11341 gtttccacga cggacaggtg atatgaatgg tcatggtctc aagggtcgtg gaccagtctg
    11401 gtgggttccc tttgaagaaa tgacagcaga aacctgccaa gcaaaaccgg atgagattaa
    11461 aagattagtc aaacgtttat cgtcagaagt agataaacta ttagaaataa aggagtagaa
    11521 atgaagaaaa gatacctagt cttgacagct ttgctagcct tgagtctagc agcttgttca
    11581 caagaaaaaa caaaaaatga agatggagaa actaagacag aacagacagc caaagctgat
    11641 ggaacagtcg gtagtaagtc tcaaggagct gcccagaaga aagcagaagt ggtcaataaa
    11701 ggtgattact acagcattca agggaaatac gatgaaatca tcgtagccaa caaacactat
    11761 ccattgtcta aagactataa tccaggggaa aatccaacag ccaaggcaga gttggtcaaa
    11821 ctcatcaaag cgatgcaaga ggcaggtttc cctattagtg atcattacag tggttttaga
    11881 agttatgaaa ctcagaccaa gctctatcaa gattatgtca accaagatgg aaaggcagca
    11941 gctgaccgtt actctgcccg tcctggctat agcgaacacc agacaggctt ggcctttgat
    12001 gtgattggga ctgatggtga tttggtgaca gaagaaaaag cagcccaatg gctcttggat
    12061 catgcagctg attatggctt tgttgtccgt tatctcaaag gcaaggaaaa ggaaacaggc
    12121 tatatggctg aagaatggca cctgcgttat gtaggaaaag aagctaaaga aattgctgca
    12181 agtggtctca gtttggaaga atactatggc tttgaaggcg gagactacgt cgattaatac
    12241 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac ctactgactg cgtcggttct
    12301 attcacaacc tcaaaacagt gttttgagct gacttcgtca gttttatctg caacctcaaa
    12361 gctgtacttt gagcagcctg cggctagctt cctagtttgc tctttgattt tcattgagta
    12421 caaaaaataa acttttctct tgcaattcca gataaatagt gtataatgga tgggtatgtg
    12481 aaaaacatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca aaggaggatt
    12541 taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct ggtaaagcta
    12601 caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc atgggattca
    12661 caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca gttgttatct
    12721 cagtttacga agataaatca tttactttcg ttacgaaaac accaccagct gctgttcttt
    12781 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taataaaact aaagttgcta
    12841 cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat ttgaacgcag
    12901 caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
    12961 ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt tgcttttcat
    13021 actaggcaag tgactgaggc ttgtacttgg gtacagcaag ggagcttaag gccgtaggaa
    13081 gagaaaaata gtagactgaa aacccgcaag acttcatcat ttcgagaagt gacgtgggag
    13141 atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa agcaaacaac
    13201 ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagtgtagaa gaagctgtag
    13261 cacttgcaaa agaaactaac tttgcaaaat ttgatgcaac tgtagaagtt gcttacaact
    13321 tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta ttgccaaacg
    13381 gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct gaagaagcaa
    13441 aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc aacgacggtt
    13501 ggttggactt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt ggacgtcttg
    13561 gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact gtaacaatgg
    13621 atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt gctgaccgtg
    13681 caggtaacgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa ttggttgaaa
    13741 actttaaagc tttcaacgaa acaatccaaa aagcaaaacc agctacagct aaaggaactt
    13801 acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt gacgtaaact
    13861 cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acgctatgtc tcaacttttt
    13921 ttgatgtacg acgggcatgt tgtatagtag atgtgtacta ttctagtttc aatctactat
    13981 agtagctcag aagtcggtac ttaaacgtgc tatatcaaaa ccagtccttg aaaaacgtgg
    14041 actggtttcg tgtttggatt attaccttga acgacatgcg ttaacagtta gttgaaccgc
    14101 cgtatgccga acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat
    14161 ttcgaaatct agtggaatga atctggaata gtccatcgag ctttctaata ctcttcgaaa
    14221 atctcttcaa accacgtcaa cgtcgccttg ccgtgcgtat ggttactgac ttcgtcagtt
    14281 ctatccacaa cctcaaaaca gtgttttgag ctgactacgt cagttccatc tacaacctca
    14341 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttggt tttcattgag
    14401 tataacacat tgttagaagt tggtttaaat ttcctaatca gtttgttcac atttaccttc
    14461 gatatattat atcccatagt taaggttggt catacagatg attatagtca tggagccgta
    14521 aaacttagtg tttctttagt tgacaaagat gccatgaaaa aaatatttgt aactgtaata
    14581 ggatattttg aaataaatat agatgaaaat atcaccgata ttctatacgt aaatggtact
    14641 gctattcttt atctttattt acgttctatt gtttcaatag tttcggcaat tgatagcagt
    14701 gaagcaatgt tgctacctat cattaatgtt ttagagttac tagataaatc tcaacctttt
    14761 gaagaagaat aatttattag ctcactaaat tgagggtaag gaaaagtaaa agcagtaaga
    14821 aaaatgtctt gcattataca gcaacctttt gggaatgagt ggatggattg aataaaattt
    14881 gattaagagt ggatgattta tctgtagatt attattggac agttagtctt gaagtagtct
    14941 aagaattagg ttataatcag tagaagcctt gctaataatg aggaggttag tttat
//
LOCUS       DAVMEF010000037        14760 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMEF010000037 DAVMEF010000000
VERSION     DAVMEF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.037"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(146..1768)
                     /gene="groL"
                     /locus_tag="VYQ30_000811"
     CDS             complement(146..1768)
                     /gene="groL"
                     /locus_tag="VYQ30_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1965960.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1784..2068)
                     /gene="groES"
                     /locus_tag="VYQ30_000812"
     CDS             complement(1784..2068)
                     /gene="groES"
                     /locus_tag="VYQ30_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1965961.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2338..2733)
                     /locus_tag="VYQ30_000813"
     CDS             complement(2338..2733)
                     /locus_tag="VYQ30_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1965962.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2811..3572)
                     /locus_tag="VYQ30_000814"
     CDS             complement(2811..3572)
                     /locus_tag="VYQ30_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1965963.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(3605..4231)
                     /locus_tag="VYQ30_000815"
     CDS             complement(3605..4231)
                     /locus_tag="VYQ30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1965964.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4247..4564)
                     /locus_tag="VYQ30_000816"
     CDS             complement(4247..4564)
                     /locus_tag="VYQ30_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1965965.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYVYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4561..4860)
                     /locus_tag="VYQ30_000817"
     CDS             complement(4561..4860)
                     /locus_tag="VYQ30_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1965966.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            4948..5028
                     /locus_tag="VYQ30_000818"
                     /pseudo
     CDS             4948..5028
                     /locus_tag="VYQ30_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(5157..5348)
                     /locus_tag="VYQ30_000819"
     CDS             complement(5157..5348)
                     /locus_tag="VYQ30_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1965967.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGAMKVSTWVYDKGEWY
                     YVSSSGSMIANNWVKDNGK"
     gene            complement(5509..6183)
                     /locus_tag="VYQ30_000820"
     CDS             complement(5509..6183)
                     /locus_tag="VYQ30_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965968.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6189..6635)
                     /locus_tag="VYQ30_000821"
     CDS             complement(6189..6635)
                     /locus_tag="VYQ30_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1965969.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6749..7252)
                     /locus_tag="VYQ30_000822"
     CDS             complement(6749..7252)
                     /locus_tag="VYQ30_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1965970.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIALVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7249..7611)
                     /locus_tag="VYQ30_000823"
     CDS             complement(7249..7611)
                     /locus_tag="VYQ30_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1965971.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7737..9536)
                     /locus_tag="VYQ30_000824"
                     /pseudo
     CDS             complement(7737..9536)
                     /locus_tag="VYQ30_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9575..10024)
                     /locus_tag="VYQ30_000825"
     CDS             complement(9575..10024)
                     /locus_tag="VYQ30_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1965972.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10148..10864)
                     /locus_tag="VYQ30_000826"
     CDS             complement(10148..10864)
                     /locus_tag="VYQ30_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1965973.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11745..13160)
                     /gene="ply"
                     /locus_tag="VYQ30_000827"
     CDS             complement(11745..13160)
                     /gene="ply"
                     /locus_tag="VYQ30_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1965974.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13171..13503)
                     /locus_tag="VYQ30_000828"
     CDS             complement(13171..13503)
                     /locus_tag="VYQ30_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965975.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13574..14182)
                     /locus_tag="VYQ30_000829"
     CDS             complement(13574..14182)
                     /locus_tag="VYQ30_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1965976.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14195..14653)
                     /locus_tag="VYQ30_000830"
     CDS             complement(14195..14653)
                     /locus_tag="VYQ30_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1965977.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4287 a         3419 c         2683 g         4371 t
ORIGIN      
        1 tgaacatcaa aaaagaaagg acgaaatttg tcctttctca agcttagctg acttcaaccc
       61 actacagttg acaaagagcc tataaaaaga agggttagtc attccctcct tttgtgtttt
      121 ttataagttg ttttctatag aaagcttaca tcatcccgcc catcatactt ggatccattg
      181 ctggagctgg ggctactggt tctggtttat tggctacgac tgcttctgtt gtcaaaatca
      241 agctggctac agatgctgca ttttgtaggg ctgaacgact cactttaact ggatcaatga
      301 taccttgatc aatcatgtta acccactcgc cagttgctgc gttaaatcct ataccaagct
      361 cagcattttt caaacgatcg ataacgatag atccttcaaa tcctgcattg tgagcaattt
      421 gacgaacggg ttcttccaaa gcacggagaa caatattacg tcctgttgct tcatctcctg
      481 tcaattccaa ggtagcaaca gctggaatca cattggcaag agctgttcca ccgcctgcaa
      541 caataccttc ttcaacagct gcacgagtag cgttgagggc atcttcaatg cggagtttca
      601 tttctttcaa ctcagtttca gttgcggctc cgaccttaat aaccgctaca ccacctgaca
      661 atttggccaa gcgttcttgc aatttttcac ggtcaaattc agaagttgta gtttcgattt
      721 gagacttgat aaccgcaaca cggtgagaaa tcgcttcagg atttcctgca ccttctacaa
      781 taaccgtgct atctttgtcc acggtcactc tcgctgcttg accaagagct tcaattgtcg
      841 catctttcaa ctcaagacca aggtcttctg tgataactgt tccgcctgtt aagatggcga
      901 tatcttcaag catagctttg cgacggtcac caaaaccagg tgccttgact gctactacgt
      961 tgaaggttcc acgaatcttg ttcaaaacaa gagttggaag agcctcgcca tccacatcat
     1021 ccgcaataat caagagtgga cgattgcttt ggagaatgct ttccaaaagt ggcaagattt
     1081 cttggatatt ggaaattttc ttgtctgtaa tcaaaatgta cggattttca aggtcagcca
     1141 ccattttttc gctatctgtc accatgtact gtgaaaggta accacggtca aactgcattc
     1201 cttctacgac ttcaagctct gtttccatac cacgtgactc ttcgatggtg atgacaccgt
     1261 ctttgccaac tttttccatt gcttcagaga tgtactcacc aactttttca gaacgagaag
     1321 atacggctgc aacttgagcg atagcttctt tattggcaac agggatggcg ttgtttttca
     1381 aagcttcaac tgctgcggca accgctgttt caatcccacg acgaataccg attggatttg
     1441 cacctgctgt gacgtttttg attccttcac ggacgattgc ttgggtcaaa acagttgcag
     1501 ttgtagttcc atcacctgcg atatcattgg tttttgaagc tacttctgat accaatttgg
     1561 cacccatatt ttcaaaatgg tcttctaatt caatttcttt ggcaatagtc acaccgtcat
     1621 tggtaatcaa tggtgagcca aatgattttt caagaacgac attgcgacct tttggtccca
     1681 aagttacttt aacagtgtct gcaaggatat cgacaccacg aaccatggct gaacgggcat
     1741 ctgatgaaaa tttaatttct tttgacatac ttactttctc cttctattcc tcaatgattg
     1801 ccaaaatgtt agcttcgcct acgatgatgt acttttcatc gccatctttg acatcaagac
     1861 ctgcgtgggc ttcaactaag acacgatctc cagttttaac acttggagca accaagtcac
     1921 cgttcaaggt acgaacacct tgtccagtag ccacaacttg agctgttttg gttttttctt
     1981 gggctgagcc tgcaaggaca aagcccccaa cggtttgttc tttttcttct atttttaaga
     2041 ccacacggtc ccctaatggt ttcaacatct gatttcctcc ataatgagat agatattatt
     2101 agcactcttt gaacttgagt gctaattcat aattctattg tatcacttgg tcagaaatag
     2161 tcaagaaaaa agtctgactt tctcaagata aaaagcctga gaccaactca gactttttaa
     2221 tgtttatact caatgaaaat caaagagcaa actaggaagc tagccgcagg ctgctcaaag
     2281 cattgctttg atgttgtgga taaagctaac gtggtttgaa gagattttcg aagagtatta
     2341 aaatggcaat tcttcctctt ccaagactaa atctgccaaa tcttggcctg cattattttc
     2401 acgcatggca cgttgagcac gactttccaa gagttggaat cctgtgacaa gtacttcggt
     2461 cacgtagttc atttggccat ttttctcaaa gcgacgggta cgcaattctc catcaacgga
     2521 aatgagacta cctttggttg cgtagcttgc caaagtttct gctagtctgc cccataggac
     2581 catattgaca aaatcagctt cacgttcacc gttttggtct ttgtaacgac ggttgacagc
     2641 gatagttgct cgcgctaccg acttgtcatt gttggttttg tgcaattctg gtgtagacgt
     2701 taaacgccca atcatgataa ctttattata catattttct tcctcctact tatctattcg
     2761 taggaaatca aaaaaagtta cagaaatttg taacttttcg aggaaatttc ttatttttta
     2821 tgaatcatga aacctgtcgc ctgttgattg gccataatgg tcatatctgt aatctgaaca
     2881 cggcgaggct gactggtcac atagactact gtgtctgcaa tatcctgagc ttgcaaggct
     2941 tctattcctt ggtaaacgga cgcagctcgc tctttatcac catgaaaacg aacagttgag
     3001 aaatctgttt cgacaatccc aggctgaatg gttgtcacct tgatatccgt tgcgatggta
     3061 tcaattcgca gtccatccga aaaggtctta accgcagcct tggtagctga gtaaacagcg
     3121 gcaccagcat aggcgtagat tcctgcggtt gaccccatat tgataatatg accttgattg
     3181 acttttatca ttgctggcaa gaaacagcga gtaaccgcca tcagaccttt aacattggta
     3241 tccaacatgg tcaacatatc caactcctca tagtcttgat agggagccaa gtcaagagcc
     3301 agtccggcgt tgttgaccaa gatatcaatc ctccctatcg tttctagaat atcagagcaa
     3361 acagtcttca ccatgaccat atccgtcaca tctagtggaa aagtccagac ggtttgattt
     3421 ggaaagctta ctgcaaactc cgacttgagg atttctaatc tgtctatccg tcgtcctgtt
     3481 agaacgacat cctcaccctg ctccagataa gcacgcgcaa tcgcttcacc gattcctgag
     3541 gttgctcccg taatcactac attttttgcc atcttatttc cttctatcag atgctgacaa
     3601 gttcctaggc agtccagtgt ttcgctgggt cgaacggtgt tccgacaact tggtcttctg
     3661 ataattcaag caccccacgt ttttgcggag catttggcaa atgcaattca cgaggactac
     3721 acatcatccc aaaactcttt tcaccacgaa gttcgcctgg gaaaatgaaa ttcccttttg
     3781 gcatcatggc accaggaaga gccacaatgg ttttcaaccc aacacgcgca ttaggtgctc
     3841 ctgcaacgat ttgcactatc ttgtcacttg cgactgcaac ttggcagatg ttgaggtggt
     3901 cactgtctgg atgagctacc atctcgacaa tttcaccaac aacaaactta ggttccttat
     3961 cattaacaat ttcttctgta aaaccttccg cctgtaattc ttggttcaaa cgagcgactt
     4021 gctcatctga taaaaagact tgaccgcgct ctgcaatttc aaacaagctc gaaacctcaa
     4081 aaatgttcca agctactgtt tccccatttt ctttgaggaa aacacgggct accttgcctt
     4141 tgcgctccac atccagtttg gcatctccgc tatttttcac gatgaccata aggacatcac
     4201 cgacatgttc tttattatat gtaaaaatca ttgtttcctt tttctcctat ttcagtcctg
     4261 ctaaaaagtc attgatttgt tgcttgcttt tacggtcgcg attgacaaaa cgaccgattt
     4321 ccttgtcctt ttctagaaca acaaggctag ggattccata tacatcccag agtttggcca
     4381 aatctatata ctggtctcta tccactcgaa taaaggtaaa ctctggatta gtctcctcaa
     4441 tttctggtaa ggcaggatag acataacggc aatcgccaca ccagtccgcc acaaaaagga
     4501 agaccttctt accgtctttt tctactaaag atgccaattc ttctaaactt gctggttgta
     4561 tcataagact tcctcctcat agactaggtc ttcattttca tagacaaaag tataatgacg
     4621 gccatcctca aaaatgacgc caccaagcaa gctctctaga ctgctttcat agacttgaac
     4681 atagagggtc gcaatttccc ccatgtctga aaaatggtct cgtacaatcg ctgtcaactc
     4741 ttcctgagtc ttcatgagct tatggtcatc tgcaactttt ttcgtagcaa gggcaaggct
     4801 cccaatacca agcagagcca gacctgccat ccacattttt ttagctttca taccattcat
     4861 tttaacacaa aaaaggcttt agaacaaatg aggaaacagc aaaaaagcaa gtaaaaagcc
     4921 tcttccttta aggaaaagga cttcttatac tcaatgaaaa tcaaagacca aactaggaag
     4981 ctagccgcag gctgctcaaa gcactgcttt gaggttgtag ataaggcgac gctgacgtgg
     5041 tttgaagaga ttttcaaaga gtattattct tattgccagg cacctaagtt gccaacgtag
     5101 taaccatcag gtgtgtaggt attgcgaagc atcttacctg atgaagccag ataatactac
     5161 ttgccattgt ctttgaccca attattcgca atcatggaac cagaagaact tacataatac
     5221 cattctccct tgtcataaac ccaagtactg actttcatgg ctcctgagca attaaaggca
     5281 aaaaaactgt ccaatatcat tcgtttttta aaagcatttg acactacatc atctacgctg
     5341 tagtacatag atacatcaaa cgtaaaagcc aaaatatcga aaaatataca acttttaaag
     5401 gcagcatttc attttttaaa gtctaacaac catagaactg aaatcagagc aaggcatcaa
     5461 tttcctactc ttagtacgtc tattttgatg gttgaagtga agattttttt atttaacgta
     5521 gacaatttcc aacttcccaa aagccacatc tccacggata gtcaaggttt tgctggttgg
     5581 ggctacagga gcgtcagcct tagctgcacc aaaggaggtt tctactttca aatctacacg
     5641 ccagtgttgt ggaacataga cggttgcatt tccgaaggcc acttcaatat ttaaagttgc
     5701 aaaatcacct agcatctctg cattatcata gtagatttta gcatccccaa aagcgacttc
     5761 cacttggtca tctacgagat cttgatcttg cttatagaag gtcccgctac caaaagcgac
     5821 ttccttatcc gtgacgactg tttttttccc attgtaccac catttttttt cattccagaa
     5881 cttacttgaa tgcgtcagat aaccaacacc aagtaccacc aagatgctag cccaaataag
     5941 agaatgattg gtaactggta acaagtcata agcgtaattt gcaatgatga gcgccagtaa
     6001 acctgtaaaa actgccgaag tgagatgacg tctaaggatg gactcaatgg acttataagc
     6061 aaaaaaaaca atacctagta aaggccatat tttaccatcc aaagaaggaa ttccaaaatt
     6121 cccttgcagc aagatccaag ctgctaaaac caataaaaca ataccaaatg ctttcttttt
     6181 catgtttttt acctcatgtt tcttagattt tcttttagga gggattggta atgccgtgag
     6241 acatgaacct ccttgtgcgt ctgataaaag gaaatggtgc ccgttcctga aaaggacttg
     6301 tccactgagt aaatctgacg gatgtttgcg atcgttgact tggaaactcg attaaaatag
     6361 cgaggcaaga tagactccaa ctcatagagc ttgaggcgaa cctcataagc ttccttctgg
     6421 ttatgagcgt agatcttgct cccttctgtt tcaaagaaga gaatttctga caggtctaga
     6481 taatattcac ctgtcccctt gtaaaaagtc aacctcggag cctttgactc ttgcaagagc
     6541 cgttgcaagt ctgcaatttc atctgtcaaa gtgggggtct tgatgacaat ttcagtctcc
     6601 tctaaattgc cgtcaatctc aattcgtaac ttcatcacat ctcctttctg actctactat
     6661 atcaaagcta agttaggaat acaagggcaa aaaagcaagt ggtcaatttg aagcagtaag
     6721 tggttgtagg actagcttaa cttacatgtc agaataggaa gaaaaggaat ccccacacca
     6781 aaccacaagc atagccaacc aagacatcct tgggataatg aacaccccct aaaaccctca
     6841 ccaaccccag caaagctgaa aagatcaagc atactaaggc aataggtaga ctagcatgca
     6901 gacaggccat ggagatgata gttgccgaaa agacatggcg actgggcatc gaccgtcccg
     6961 aactatcctt atcaagaagg ggacagatat cccaagtttc ataaggtctc gggtgattga
     7021 ttttcttacg aaacagggac aaggccacaa aacctgatgc aggaataagc acataaaccc
     7081 caacttgctg accaagtcct agttgcaggt aagtggtgac tagcaagatc agatagacca
     7141 ggggcatggc gactgtcatc aaacgattga aactgcgtaa taggaataaa agaattggac
     7201 gactagttag tctggagctg atatttcgat accattcttg ataatttttc atttatgctt
     7261 ctcatacttt tctagaactt gttgagatgt tttccccttg gcaagccaat ccactagctt
     7321 gtccaagcga cgtatttcct gcatcagagg atcatcaatt tcctcaatac ggacaccgca
     7381 aatcttccct gtaattgcaa ttcgttcagg gacataatat ggtgcttgtc taaaaaaatc
     7441 tccgtaagta acatccctat ccagacaagc aagaacatca ctcacttcat accctgtcaa
     7501 ccaactggtc acttggtgaa ctgactctgc ctttccaccc tttcgctcaa cttttgcaat
     7561 caaggctaag tagacagcag caaatttcat attgtataat ttctgactca tacttctctc
     7621 tttctatgtg tactagtata aataagaaaa agaaggccgt caagccttct tttgatttat
     7681 tcttctgctt catcttctgt aaattgacta ttgtacaagt cagcgtagaa gccaccttac
     7741 gccatcagtt cctcatagtt gccttgctcg atgatatttc catctttcat gaccaagatc
     7801 aagtctgcat ttcggatggt tgacaagcgg tgggcaatga caaaggatgt gcgtccttcc
     7861 atcaaacggt ccatggcttt ttggatcaat tcctctgtcc gtgtgtcaac agaagaagtc
     7921 gcctcatcca aaataaaaag cggtgcatcc ttaagaaggg cacgagcaat agtcaatagt
     7981 tgtttttgtc ctacagacaa ggtcacggtg tcatccaaga tggtatcata gccatctggc
     8041 aaggtcataa taaagtggtg aattcccaca gccttactag cttccatcat tcgttcatca
     8101 ctaatctcta tttgattata gatgagattg tctcgaatag ttccttcaaa gagccaggta
     8161 tcctgcaaga ccattgaaaa ggcatcatgc acttctgaac gcgtcatagc cttggtatcc
     8221 acaccatcaa tgtgaatact tcccttatca atctcataga atttcatcaa aagattgaca
     8281 atggttgtct taccagctcc agtcagccca acaatggcaa ccttctgacc agcatgagct
     8341 gtcgcagaga agtcatagtc ttgaacattg acaccgtcca ccagaatttc tcctgctgac
     8401 acgtcgtaga aacgtggaat cagattgacc agagttgatt taccagaacc tgttgaccca
     8461 ataaaggcca ctgtttgacc agtttctgct ttaaagctaa catgttcaat aactgcctcc
     8521 gaatttgccg catagcggaa ggtcacatcc ttaaactcga cctgaccttt gaagttttca
     8581 tcagtcagct gcacttgaac agggttttgg atagaagaat gcaaatctaa aacttgatta
     8641 atccgcttag cagagaccat agttcgggga agaacgatga agagtgctcc catgagaagg
     8701 aagcccatga caacctacat ggcataagac atgaaaacaa tcatgtcact aaagagaggc
     8761 agacgcgcta tcggagcagc gtcgttaatc acataggccc caatccagta aatcgctaca
     8821 cccaaaccac ttgaaatccc catcatgata ggattcaaaa tagccataag acggttgaca
     8881 aacaaattca aacgggtcaa ttcatcattt actgctgcaa atttttcatt ttgataatcc
     8941 tctgcattgt aggcacgaac gacacgaata cctgttaaac tctcacgagt gatactgttc
     9001 agtttatctg tcagcccctg aatcaaggac tgttttggaa aggctagcgt catcaaaacg
     9061 gtcgtcatca ggacgttgat aatcactgcc acaagtacgg cccagagcca gtattctgaa
     9121 tgacctaaaa tcttcccaat agcccagata gccataattg aaccacgcgt taccacttgc
     9181 aagcccatag taatcaacat ttgaacttga gtaatgtcat tggtagtacg cgtcaagagg
     9241 ctaggaattg aaaatttctt aatctctgtc tgcgagtaat ccaaaactcg gttaaaaata
     9301 tcacttctca gcctactagt ataagaagcc gccactcggg atgcaaaaaa tccaactgca
     9361 actacggaca agaaggcaag aaaggacatt cccatcatca tgcttgccgg ctgccacaac
     9421 tcatctaaat tagtttcttg actacctagc aaatccgtaa ttttcgagat ataggtcggc
     9481 acttccaact ctagatagac cgaaaagcaa gtaaagagaa tggctagtaa aatcatcccc
     9541 cattcttttc tactaattct tttggctaat ttctttattc tctcctccta ttcccttgat
     9601 attttgcctg tagttgaccg agaactttct caaaaatcag taattcatct tcatcaatgt
     9661 cttccatcaa ctgcttgtct atgcgttcaa aaaaagcctt aacctgttgc atctgagaac
     9721 gtgctttgtc cgtcagacga acaaacttag cccgcttatc aacaggactc gcctccaatt
     9781 ccaccaaacc attttgcact atacgcttaa ccagattact agcaacagac ttggtaatat
     9841 tgagttcctg ctcgatatct ttaatcaaga ccaagtcttg gtttttctcg cgattatcca
     9901 aaaaacgcac aacctgacct tgcggcccac ccataaattc aatgccgcaa cgtttggctt
     9961 ccttttgcac catcaggtga atttgatgac caaaacgctt aaagactaac atcggtttat
    10021 ccataatctc ccccttctaa ataaaaatag ttctctggag aataattaaa tttctatgag
    10081 aactattttc ttgattaaaa aaatcccaag tgattttctc acttaggatc atgttctata
    10141 ggttaaatta aaacccatct acgttcgtat aaatcttttg gacgtcttcg tcgtcttcaa
    10201 gaacgctgta aagtttttca aaggtttcaa ggtcttcgcc cgacaattcc acttctgact
    10261 gaggaatcat ttccaattca gtcacttgga attcttcaat accagactca cggagagcaa
    10321 cgatagcctt gtgaaggtca gttggcgctg tgtaaactgt gattgtacct tcttgtgctt
    10381 ctacgtcatc cacatccaca tccgcttcga gcaattgctc aaagactgcg tccgcatctt
    10441 cacctccaaa tacaataaca cctttgttgt caaaaaggta agaaacagaa cctgaagcgc
    10501 ccatgtttcc gccgttttta ccaaaggctg cacggacatt ggctgctgta cggttaacgt
    10561 tagaagtcaa agtatccaca attagcatag agccatttgg cccaaaacct tcgtaacgtc
    10621 cttctgtaaa ggtttcgtct gtgtttcctt tggctttatc aatcgcttta tcgataatgt
    10681 gttttggcac ttgggcttgt ttagcacggt cgataacgaa tttcaaagct gagtttgatt
    10741 ctggatctgg atcacctttt ttagctgcta catagatttc tacaccaaat tttgcatata
    10801 ctttagagtt ggctccatct ttagccgttt tcttggctac gatattggcc catttacgtc
    10861 ccattaggaa tctccttttt tcacatttta atctttctta ttataacaca agtttttttg
    10921 attttcacta gaggaaatgg attttattag caaatcaagc taggatagca ctttacctgc
    10981 taagatggtc ttgcctttct atctttatca acagacactc atccacattc aaaaaacaaa
    11041 ctagaccatt atctgcaaat agaaagtttc agccaagttt gacaaagtca gctcaaatta
    11101 ctgtttgaag tttgtagata taagcgacaa aaacaatcat actgcacctt ttgttgacag
    11161 tctactccag acatatcata gttcaagtaa atactttgaa atccaacagt tcttataagc
    11221 gctattgtat tctaagaaat caatagaaga gtttctaagc aaacctctaa tactcaataa
    11281 aaatcaaaga gcaaactaga aagctagccg caggttgctc aaaacactgt tttgaggttg
    11341 cggatagggc tgacatggtt tgaagagatt ttcgaagagt ataatttacg tgttcctaag
    11401 atggagaagt tagactagta cactggcact tctaaaccat tgctagcaat tgatttgttc
    11461 atatttaatt tcattttttc cataaatggg tattagatat aaacagcaaa atatttccga
    11521 tacgtgtcgt tcttgaattt ccaatcatct aaaacaagta aaggataatc aatcccctgt
    11581 atatcaagga attggctacc ctttttactt ttttacacat tctgtttgat agattcattt
    11641 taacatcacg agcatactcc aatggaaatc gctaggcaag agataaactt tcagatatcc
    11701 gcagagagat catcgcctct ttttgtcgca agcattctcc tctcctagtc attttctacc
    11761 ttatcttcta cctgaggata gagagttgtt ccccaaatag aaatcgtccg cttacgcact
    11821 agtggcaaat cggttttttc ataaaccgta cgccaccatt cccaggcaag cccggtacac
    11881 tctctaattt tgacagagag attacgaaca ttccctttta aaggaatact agtggtaaag
    11941 tgagccgtta aatcctgccc atttctgtcc caagccttag gagtcaagac ttccttacct
    12001 tgatgatcat aggataattc attccaagta atataatatt gggcaacata ggcaccacta
    12061 tgatccagca gtaaatctcc gtttctgtaa gctgtaacct tagtctcaac atagtctgta
    12121 ctattttgaa aggtcgcaac tacattgtca cgtaaaaaag aagttgtata ggaaatcggc
    12181 aagcctggat gatctgctgt aaagcgactg ccttcttgaa tcaagtcctc taccatatcc
    12241 accttgcctg ttacaactcg ggcacccgaa cttgggtcgc cccctaaaat aaccgccttc
    12301 acttctgtat tgtccaaaat ctgtttccac tctgtctgag gagctacctt gactcctttt
    12361 atcaaagctt caaaagcagc ctctacttca tcactcttac tcgtggtttc caacttgaga
    12421 tagacttggc gcccataagc aacactcgaa atatagacca aaggacgctc tgcagaaatt
    12481 cctctctgtt ttaaatcctc taccgttaca gtatcttgaa acacatctcc tggattttta
    12541 acagcatcta cgctgactgt ataataaatc tgcttaaaat taacaatctg aatctgcttt
    12601 tcgcctgaat ggacagagtt aaaatcaata tcaagagaat tccctgtctt ttcaaagtca
    12661 gaaccaaact tgaccttgag ttgttccatg ctgtgagccg tgattttttc atactgcatt
    12721 ctagctggga cattattgac ctgaccataa tcttgatgcc acttagccaa caaatcgttt
    12781 accgctccgc gaacacttga attgctgggg tcttccactt ggagaaagct atcgctactt
    12841 gccaaaccag gcaaatcaat actataagtc atcggagcac gatcgaccgc aagaagagtg
    12901 ggattattct ctaacaaggt ctcatccact acgagaagtg ctccaggata gaggcgactg
    12961 tcgttggtag ctgttacaga aatatcactt gtatttgtcg acaagctccg cttctttctt
    13021 tcgataacaa caaactcatc gggtagctga ttaccctctt tgatgaaacg attttcaata
    13081 ctttctccct gatgggtcaa gagtttcttt ttatcgtaat tcatagctag tataaagtca
    13141 tttactgctt tatttgccat cttctacctc ctaataagtt cctggattga gttgcataaa
    13201 ctcagacttg ttcagcgaaa tcagccgtgg ttggactaag taatccaaaa tttcctcgta
    13261 caattcttct gagacattgc gtcgccgtct ggctaaataa gaagtcggaa tgaccgtatt
    13321 atccaacata aataccttat ctaagtcaat caaggttggt cttgtaaaag gattacgagc
    13381 tagatccggc tcttctatca taaagttctt gaccaaacgt ctggtcaaga gagctggttt
    13441 gaaggtctga tttttaacca actctttgtt tttagtcatg ctgttgtcaa tacagatata
    13501 catatgattc ttcacagcca aatcgctact aatagtcgga aaaggcaaat aaagagctac
    13561 aacatctcct ctcttaatca agcaagagca cccccttttc tcctaatgta acatagacag
    13621 gattgaccaa gtcttctgat tgactcagaa tttccaaagt ttgagtttgg cgcgctgtca
    13681 atttagtagc atcttgtctc ttcaatacaa aatgcttgtc gccaataacc ttgacaatat
    13741 aatccttctc caaagctgac tggtaaatcc acatcagatg ttgtctgtcc tgagaactca
    13801 agagagaagg attttcaagc ctcccgatag tctgataaaa atcaaaaaca ggagctaact
    13861 cctgccaatc tgattggcta gttgtcaagg ctagaaaaag ggctttgcga gctgatactt
    13921 cttggttagc cttgagagtt actttcccct ccaagttttt tagaaatcgg gaaactccag
    13981 aaagcaaatt tttctctaac tgcgagaaat aaaaaccttt cgttcccaga cataagtctt
    14041 tcatgtcgct ttctctagca aataagagct caaacatttg atagtaaaag aaaaatatct
    14101 ggcactgggt cgcgctcatc ttttccttat cggcttcttt ttttaaccag agcaagggcg
    14161 acaggtagct ggattgagac atttcctcta cctcctactc ttttttaact ggagcatctg
    14221 cactagctgc cacttctttt gactggatac tttcccactg gttaatctcc tctgagataa
    14281 gaccttcgca tgtcttgaca aatagggcaa aagccttggt ctttcctgca tatttctccg
    14341 tttggcattg atagaggaat ttttctttct ccaggagttg cgcagttttt tggtaagaaa
    14401 tccaattttc ctttgcatta tacaaattga taatcccctc acacagcaag ccgagactgg
    14461 ataaggcaac cgaaatcaaa cggtagcgat cacctggcat aggaatagca caaaagacag
    14521 ctatgaggaa acctgccacg atttctgtta tttttaatac cttatagcgc ctacgatgtt
    14581 gaacgctttt ctttaaaaaa tgagctatct gtacgtctaa tcgctctgtc aggtacattt
    14641 cttctggcgt catattcgta actcctttca tttactttga taatcatagt ataacatatt
    14701 tgaatatcaa agtatagtaa aaactggact ataacttatt agacttcctg cgaaacaaaa
//
LOCUS       DAVMEF010000038        14081 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMEF010000038 DAVMEF010000000
VERSION     DAVMEF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.038"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..832
                     /locus_tag="VYQ30_000831"
                     /pseudo
     CDS             1..832
                     /locus_tag="VYQ30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(896..1978)
                     /locus_tag="VYQ30_000832"
     CDS             complement(896..1978)
                     /locus_tag="VYQ30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1965978.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1975..2952)
                     /locus_tag="VYQ30_000833"
     CDS             complement(1975..2952)
                     /locus_tag="VYQ30_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1965979.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDTGSVHELLYTMRQTAGIRFEAEK"
     gene            3185..3562
                     /locus_tag="VYQ30_000834"
                     /pseudo
     CDS             3185..3562
                     /locus_tag="VYQ30_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            3797..5188
                     /locus_tag="VYQ30_000835"
     CDS             3797..5188
                     /locus_tag="VYQ30_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1965980.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5291..5368
                     /locus_tag="VYQ30_000836"
                     /pseudo
     CDS             5291..5368
                     /locus_tag="VYQ30_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5444..6286
                     /locus_tag="VYQ30_000837"
     CDS             5444..6286
                     /locus_tag="VYQ30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1965981.1"
                     /translation="MDTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREELFVTTKLWNSQQTYEQARQAFEESLEKLGLDYLD
                     LYLIHWPNPKPLRENDQWKIRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     KILPAVNQIRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTVSRIQANLDCFGIELSHEEHETLKTIAVHSGAPRVD
                     DMDF"
     gene            6559..7320
                     /locus_tag="VYQ30_000838"
     CDS             6559..7320
                     /locus_tag="VYQ30_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1965982.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWLSFLIVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7515..8432
                     /gene="glyQ"
                     /locus_tag="VYQ30_000839"
     CDS             7515..8432
                     /gene="glyQ"
                     /locus_tag="VYQ30_000839"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1965983.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISNQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            8644..10680
                     /locus_tag="VYQ30_000840"
     CDS             8644..10680
                     /locus_tag="VYQ30_000840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1965984.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIHSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10722..10979
                     /locus_tag="VYQ30_000841"
     CDS             10722..10979
                     /locus_tag="VYQ30_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1965985.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(11202..12446)
                     /locus_tag="VYQ30_000842"
     CDS             complement(11202..12446)
                     /locus_tag="VYQ30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1965986.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(12465..13070)
                     /locus_tag="VYQ30_000843"
     CDS             complement(12465..13070)
                     /locus_tag="VYQ30_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1965987.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEETDGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(13130..14053)
                     /locus_tag="VYQ30_000844"
     CDS             complement(13130..14053)
                     /locus_tag="VYQ30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1965988.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
BASE COUNT         4270 a         2551 c         3106 g         4154 t
ORIGIN      
        1 ctctccttca agaaacacgt ggtggtcgta accatgcata tatgacagtt gaggaagaga
       61 aagcctttct tgcccgccat ttgaaggcta cagaggcagg agaatttgtt acaatacctt
      121 atttcaggct tatatcgttc ctacacacgt gatgccttct atcaactgtt gaagcaccat
      181 ggttggcgaa atattatgcc acgtccagaa catcctagga aagcagacgc tcaaaccatt
      241 gtcgcgtcta aaaataaaat ctcaattcaa gaagacaaga aagcgcttta aaaccagtag
      301 accttttcat aaggttcgct tgatgtacca agatgaggct ggtttcggta gaatcagtaa
      361 actgggatct tgttggtctc caataggagt aggtccacat gtccatagtc actatatacg
      421 agaatttcgc tattgttatg gagctgttga tgccccatac aggcgaatca tttttcttaa
      481 tagctggtgg atgtaatact gagtggatga acgtcttttt agaagagctt tcacaagctt
      541 atccagatga ttatttttta ctcgttatgg acaatgctat atggcataaa tcaagtacct
      601 taaagattcc gactaatatt gattttacct ttattcctcc atacacacca gagatgaacc
      661 cattgaacaa gtgtggaaag agattcgtaa acgtggattt aagaataaag cctttcgaac
      721 tttggaagat gtcatgaatc aactccaaga tgtcatacaa ggactggaga aggaggtgat
      781 aaagtccatc gttaatcgga gatggaatag aatgcttttt gaaagcagat gagtataaaa
      841 ttgaattgct tataaaaaag ctccatacac tggatgtgta tagagcaatg gggctttatt
      901 tgatatagag ttcttggttt tttaggacaa tttctcggat acttgcaaac tttttaagtt
      961 ttttgatttc ttctggatga gtgacgagag taataacata tccttccttg cccatacggc
     1021 cagtacggcc agcacggtgt gtgtaggttt cgctatctct aggaatatca aagtttacga
     1081 cacattctag gctatcgata tcaattccac gagccaaaag gtcagttgca agaagcaggg
     1141 ttagttggtt atctttaaac ttttctaaga tgatttttct aaatttaaca ttaacatcac
     1201 tagcgaggga aacagccaat atatcacgat actgtagttt ttcctcggca ttcccaaggt
     1261 ctgacaggct attgaagaag actagaccac ggaaatcctc tacatgagcc agttttcgca
     1321 gcatatccac tcgatgacgt tggtctacct gcatgtagaa atgctggata ttgtccaatt
     1381 tttgatcaga gagatcaata gtgcgtgtat tcggcacaat cttttcttgg tcaaacttgg
     1441 tcgtggcact catgtagacc agttggtggt cacgaggtgc gtagtgagtg attttttcta
     1501 caaagtgaat ctgagaatca tctagtaatt ggtcaaattc atccaggatg atggtttcca
     1561 cattcatcat cttgattttt ttaagtttaa tgagttcaaa gatacggcca ggagttccaa
     1621 tcagaatttc tggccccttt ttaagacgtt caatttgtcg tttctgactt gaacctgaaa
     1681 ggaagagttg agcagtcaat ccgatagctt ctgcccacgt tttacataca tcaaaaatct
     1741 gtccagcaag ttccgtattt ggtgctagaa tcaagagttg ttgggctttt ttcttttgta
     1801 gtctgagaag acttggtagg agatacgcta gggtcttacc agttccggtt tggctcactc
     1861 ctaggaggtt ttctccagca agaaggggct caaatagttg agtttgaatg ggggtgaatt
     1921 cttggaaacc gagttggtca ctcagttctt gccattcagt cggtagtttg gttttcattt
     1981 ttctgcctca aatctaatgc cagcagtctg gcgcatggta tatagtagct catgaacaga
     2041 gcctgtatca tacagccaag tttggtagag attcagatct ggttgctgga tcatgtgtgc
     2101 aaatgcagcg acttcctcag tcatcgtatg aggagcctgt tggataggaa gctggacttg
     2161 atttccttgg tggtcggtaa aaatagctga gcgaatatgc tcaatcgtgt tgagagtcaa
     2221 ggttccatct gttgtataaa tctcgcaagg aagattggaa gtgatgtttt ttccagcctt
     2281 gatgtgaact tgatagtctg ggtagaagag gataccatct ccatttaggt caatgctatt
     2341 gtcaagctgt tgagcatggt aagtcgcgtc attggctttt ccaaaaagac gaacagcagc
     2401 atagagggga taaatcccca aatccatgag ggctccacca gcaaaacggt ctgaaaagac
     2461 atttggtgtt tgtccagtca acaagtcagg catcttggaa gagtatttgg catagttgaa
     2521 atctgctcct aacacttgct tatctgctaa aaagtttttg atagtagtaa aggctttctc
     2581 gtggtaatta cgagctgctt caaagataaa acagttattt ttttcagctg tttgaatcaa
     2641 atcaaaccat tcttgtggtt gagtgacagc tggcttttcg agaataacat gtttaccagc
     2701 agacaaggca gcttttgcct gagcaaaatg taaggagttt ggactggcga tatagactaa
     2761 atcaaaagaa gatttgaaga agacttctaa ttgatcgaat agttggatat tctgatagcg
     2821 agaagcaaag gttgctgcag tttctagttt tctagaatag attgcgacca gttggtattc
     2881 tccactggta tgggctgctt ctatgaaatg atggctgata gcgccagttc cgatgacacc
     2941 taattttagc ataaatactc cttttccgat tttaaatcct tctttcatta taacatagat
     3001 agacgggact atccaataga gaggagaaaa tttcaaataa gctattagct ttcttctgaa
     3061 gaaaagcttt cttttccgaa taaatagata gaagcataga atctagcaaa cctagattta
     3121 aaaatgtgct ataatagaag gaggaaaagg aggattctca gacatccggg tatcagccca
     3181 actaatgatt tgtcaattta tccgcgatat gctggacttg ccagcaaaaa atgtgacgat
     3241 tttggaggga agtaacattc acgtcttgcc ttccatgccc tactcagcgc aggatttcta
     3301 tactagtata gacgtcttgg cggagttaga taatggaatc caagttatca tcgaaattca
     3361 ggttcatcat cagaattttt tcatcaatcg cctatgggct tatctgtgta gttaggttaa
     3421 tcaaaaccta gaaaaaattc gccaacgtga aggtgatacc caccagagct acaaacaaat
     3481 cgcactagta tacgctatcg caattgtcga tagtaattac ttctcagatg acctagcttt
     3541 tcatagtttt atagtaaaat gaaatgagaa caggacaaat cgatcaggac agtcaaatcg
     3601 atttctaaca atgttttaga agtataggtc tactattcta gcttcaatct actagaaatt
     3661 ccatagatag aaaactacat aatctctaca gatacggatg ttggagttga tgtaagatgc
     3721 tttggcttgc tagaggaatt gtggattgcc aaattgtatc attgaaatta ttgctcaaat
     3781 ttgttatgat ataaatatga ataaaagtag actaggacgt ggcagacacg ggaaaacgag
     3841 acatgtatta ttggctttga ttggtatttt agcaatttct atttgcctat taggcggatt
     3901 tattgctttt aagatctacc agcaaaaaag ttttgagcaa aagattgaat cgctcaaaaa
     3961 agagaaagat gatcaattga gtgagggaaa tcagaaggag cattttcgtc aggggcaagc
     4021 cgaagtgatt gcctattatc ctctccaagg ggagaaagtg atttcctctg ttagggagct
     4081 gataaatcaa gatgttaagg acaagctaga aagtaaggac aatcttgttt tctactatac
     4141 agagcaagaa gagtcaggtt taaagggagt cgttaatcgt aatgtgacca aacaaatcta
     4201 tgatttagtt gcttttaaga ttgaagagac tgaaaagacc agtctaggaa aggttcactt
     4261 aacagaagat gggcaacctt ttacacttga ccaactgttt tcagatgcta gtaaggctaa
     4321 ggaacagctg ataaaagagt tgacctcctt catagaggat aaaaaaatag agcaagacca
     4381 gagtgagcag attgtaaaaa acttctctga ccaagacttg tctgcatgga attttgatta
     4441 caaggatagt cagattatcc tttatccaag tcctgtggtt gaaaatttag aagagatagc
     4501 cttgccagta tctgctttct ttgatgttat ccaatcttcg tacttactcg aaaaagatgc
     4561 ggccttgtac caatcttact ttgataagaa acatcaaaaa gttgtcgctc taacctttga
     4621 tgatggtcca aatccagcaa cgaccccgca ggtattagag accctagcta aatatgatat
     4681 taaagcgact ttctttgtgc ttgggaaaaa tgtttctggg aatgaggact tggtgaagag
     4741 gataaaatct gaaggtcatg ttgttggaaa ccatagttgg agccatccga ttctctcgca
     4801 actatctctt gatgaagcta aaaagcagat tactgatact gaggatgtgc taactaaagt
     4861 gctgggttct agttccaaac tcatgcgtcc gccttatgga gctattacag atgatattcg
     4921 caatagcctc gatttgagct ttattatgtg ggatgtggat agtctggact ggaagagtaa
     4981 aaatgaagca gctattttga cggaaattca gcatcaagta gctaacggct ctatcgtttt
     5041 gatgcatgat attcacagtc cgacagtcaa tgccttgcca agggtcattg agtatttgaa
     5101 aaatcaaggt tatacctttg tgaccatacc agagatgctc aatactcgcc taaaagctca
     5161 tgagctgtat tatagtcgtg atgaataagc aagaaaaaat aggtctgtca gatatgtgat
     5221 agacttattt tttacaaaag atagtactac ttaaaaaatg ttttatgcta taattgatga
     5281 ataatactca atgaaaatca aagagcaaac taggaaacta gctgcaggtt gctcaaaaca
     5341 gtgttttgag gttgtggata gaactgacga agtcagtaac aatacctacg gcaaggcgac
     5401 gttgacgtgg tttgaagaga gtataaaata gaaggagaag catatggata cctatcaatt
     5461 aaataatgga gtagaaattc cagtattggg atttggaact tttaaggcta aggatggaga
     5521 agaagcctat cgtgcagtgt tagaagcctt gaaggctggt tatcgtcata ttgatacggc
     5581 ggcgatttat cagaatgaag aaagtgttgg tcaagcaatc aaagatagcg gagttccacg
     5641 agaggaattg ttcgtaacta ccaagctgtg gaatagccaa caaacctatg agcaagctcg
     5701 tcaggctttt gaagagtctt tagagaaact gggcttggat tatttggatc tgtatttgat
     5761 tcattggcca aatccaaaac cgctcagaga aaatgaccaa tggaaaatcc gaaatgcaga
     5821 agtttggaga gcgatggaag acctctatca agaagggaaa atccgtgcta tcggtgttag
     5881 taattttctt ccccatcatt tggatgcctt gcttgaaaca gcaaaaattc ttccggcggt
     5941 caatcaaatc cgcttggcgc caggtgtcta tcaagaggaa gttgtagctt actgtagaga
     6001 aaaaggaatt ttattggaag cttggggacc ttttggtcag ggagaactgt ttgatagcaa
     6061 gcaagtccaa gaaatcgcag caaatcatgg aaagtctgtt gctcagatag ccttggcctg
     6121 gagcttggca gaaggatttt taccacttcc gaaatctgtt acagtatctc gtattcaggc
     6181 taatcttgat tgctttggaa ttgaattgag ccatgaggaa catgaaacct taaaaacgat
     6241 agctgttcat tctggtgctc cacgagttga tgatatggat ttttagaaaa tcataaaaag
     6301 aattgtacat tattctaatt tttgatataa tagtcagcag gaaagaaagt cttatggcgt
     6361 tctttaagcg agcttgggat agtgggagcc aagtagagca aaataaagaa ctggcgcttt
     6421 ctgtactatt ttcaaaaaca atgaagtaat aaattagggt ggaaccgcgt ttctgacgcc
     6481 cctaggttaa atcaacctag gattgtcaga tgtggttctt ttgcttattc agtctattgt
     6541 gtgaaagaaa ggagagacgt ggaaaacctt tatcttgtaa aagacgatag tcaactagct
     6601 gcatttcgtg attttgtagt aagaaatact gaaaagttga aagattatca atctttttta
     6661 aagaatgaac ttgcagtctg tgatttaccg caagccgtta tttggtcaaa ttttaatgct
     6721 gctacacaga ttattaggga aagcgctgtt ccagcctata caaataatag acgaatggtt
     6781 atgacgcctg atttagctgt ttggaaagag ttgtatttgt atcaattgat ggactacgag
     6841 tgttctgagc aaactcaagc aatagaaagt cactatcatt ctttatctga aaatttcctc
     6901 ttacagattg taggacatga gttagctcat tggtcggagc attttttaga tgattttgat
     6961 ggttatgact cttatatctg gttcgaagag gggatggttg aatatattag tcgcaagtat
     7021 ttcttgacag aagaggaatt tcaagcggaa aaaatttgta atcaatctct agtagaactt
     7081 tttcagaaga agtatggttg gcattcattg aatgattttg gttcttcgac ttatgataag
     7141 aactatgcaa gtatttttta tgaatattgg ctcagttttt tgatagtaga taagttggta
     7201 gaaaatttag gtagtgtaca agcggtcttt gattcttatc atctatgggc aaatacagaa
     7261 aaaacttttc ccttgttaga ttggtttgtt cagcagaaat taattgaaaa agaaatataa
     7321 aaactaaagg agtaaacaat gtctaagaaa ttaacatttc actgcatcag tggcagagac
     7381 ctccttacag tcgggctgct ccacgctcag cactagagtg cctgagctag acgcagtact
     7441 aactcgtctt gcctcgtata atcgacgagg cagactcgtg tcgcaagaaa ttatttttta
     7501 ttaaggagta ttcaatgtct aagaaattaa catttcaaga aattattttg actttgcaac
     7561 aattttggaa tgaccaaggt tgtatgctta tgcaggctta tgataatgaa aaaggtgcgg
     7621 gaacaatgag tccttacact ttccttcgtg ctatcggacc tgagccatgg aatgcagctt
     7681 atgtagagcc atcacgtcgt cctgctgacg gtcgttatgg ggaaaaccct aaccgtctct
     7741 accaacacca ccaattccag gtggtcatga agccttctcc atcaaatatc caagaacttt
     7801 accttgagtc tttggaaaaa ttgggaatca atcctttgga gcacgatatt cgttttgttg
     7861 aggacaactg ggaaaaccca tcaactggtt cagctggtct tggttgggaa gtttggcttg
     7921 acggaatgga aatcactcag ttcacttatt tccaacaagt cggtggattg gcaactggcc
     7981 ctgtgactgc ggaagttacc tatggtttgg agcgcttggc ttcttacatt caagaagtag
     8041 actctgtcta tgatatcgag tgggctgatg gtgtaaaata cggagaaatc tttatccagc
     8101 ctgagtatga gcactcaaaa tattcatttg aaatttcgaa ccaagaaatg ttgcttgaaa
     8161 actttgataa gtttgaaaaa gaagctggtc gtgcattaga agaaggcttg gtacaccctg
     8221 cctatgacta tgttctcaaa tgttcacata cctttaatct gcttgacgcg cgtggtgccg
     8281 tatctgtaac agagcgtgca ggctatatcg ctcgtatccg taacttggcc cgtgttgtag
     8341 ccaaaacctt tgttgcagaa cgcaaacgcc taggttaccc acttttggat gaagaaacac
     8401 gagttaaact tttagcagaa gacgcagaat aaagagagtg acaaattacg aaaatgggcg
     8461 aacagagtga gccctgagcc agttgccaca gtgatgaagg tatccttagt gaaactaagg
     8521 atactaggca aaattggaga cttttggctc caattttagc aatgaaacaa cgaagttggt
     8581 tgcttgcgtg ccaatcacat aaggcaaact ggaaaataaa aagatacttt tcggagaaaa
     8641 aacatgacaa aaaacttatt agtagaactc ggtcttgaag aattaccagc ctatgttgtc
     8701 acaccaagtg aaaaacaact aggcgaaaaa atggcagcct tcctcaagga aaaccgcctg
     8761 tcttttgaag ccattcaaac cttctcaaca ccacgtcgtt tggctgttcg tgtgactggt
     8821 ttatcagaca aacagtctga tttgacagaa gatttcaagg gaccagcaaa gaaaattgcc
     8881 ttggatagtg atggaaactt caccaaagca gctcaaggat ttgtccgtgg aaaaggcttg
     8941 actgttgaag atatcgaatt ccgtgaaatc aagggtgaag aatatgtcta tgtcactaag
     9001 gaagaagttg gtcaagcagt tgaagccatt gttcctggtg ttgtagatgt cttgaagtca
     9061 ttgactttcc ctgtcagcat gcactgggct ggaaatagct ttgaatacat tcgtcctgtc
     9121 cacactttaa ctgttctctt ggatgagcaa gagtttgact tggatttcct tgatatcaag
     9181 ggaagtcgtg tgagccgtgg ccatcgtttc ttggggaaag aaaccaaaat tcattcagca
     9241 ttgagctatg aagaagacct tcgtaagcag tttgtaatcg cagatccatg tgaacgtgag
     9301 caaatgattg ttgaccaaat caaggaaatt gaggcaaaac atggtgtacg tatcgaaatt
     9361 gatgcggatt tgctgaatga agtcttgaat ttggttgaat acccaactgc cttcatggga
     9421 agttttgatg ctaaatacct tgaagttcca gaagaagtct tggtgacttc tatgaaggaa
     9481 caccagcgtt actttgttgt tcgtgatcaa gatggaaaac tcttgccaaa cttcatttct
     9541 gttcgtaacg gaaacgcaga gcgtttgaaa aatgtcatca aaggaaatga aaaagtcttg
     9601 gtagcccgct tggaagacgg agaattcttc tggcgtgaag accaaaaatt ggtgatttca
     9661 gatcttgttg aaaaattaaa caatgtcacc ttccatgaga agattggttc ccttcgtgaa
     9721 cacatgattc gtacgggtca aatcactgta cttttggcag aaaaagctgg tttgtcagtg
     9781 gatgaaacag ttgaccttgc tcgtgcagca gccatttaca agtttgactt gttgacaggt
     9841 atggttggtg aatttgacga actccaagga attatgggtg aaaaatacac ccttcttgct
     9901 ggtgaaactc cagcggtggc agctgctatt cgtgaacact acatgcctac atcagctgaa
     9961 ggagaacttc cagagagcaa ggtcggcgca gttctagcca ttgcagacaa attggatacg
    10021 attttgagtt tcttctcagt aggattgatt ccatcaggtt ctaatgaccc ttatgccctt
    10081 cgtcgtgcaa ctcaaggtgt ggttcgtatc ttggatgcct ttggttggca cattgctatg
    10141 gatgagctga ttgatagcct ttatgcattg aaatttgaca gtttgactta tgaaaataaa
    10201 gcagaggtta tggactttat caaggctcgt gttgataaga tgatgggctc tactccaaaa
    10261 gatatcaagg aagcagttct tgcaggttca aactttgttg tggcagatat gttggaagca
    10321 gcaagtgctc tcgtagaagt aagcaaggaa gaagatttta aaccatctgt tgaatcactt
    10381 tctcgtgcct ttaacctggc tgagaaggca gaaggggttg ctacagttga ttcagcacta
    10441 tttgagaatg accaagaaaa agctttggca gaagcagtag aaacactcgt tttatcagga
    10501 cctgcaagtc agcaattgaa acaactcttt gcgcttagcc cagtcattga tgctttcttt
    10561 gaaaatacta tggtaatggc tgaagatcag gctgtccgtc aaaatcgttt ggcaatcttg
    10621 tcacaactaa ccaagaaagc agctaagttt gcttgtttta accaaattaa cactaaataa
    10681 aatttgataa acggacttta tcttattaca aaggagaaga aatggatccg aaaaaaattg
    10741 ctcgtatcaa tgagcttgct aaaaagaaaa aaacagaagg cttaacacca gaagaaaaag
    10801 tggaacaagc caaactacgt gaggagtaca tcgaaggtta tcgccgcgct gttcgtcacc
    10861 acattgaagg aatcaaaatt gtggacgaag aaggaaacga tgttacacca gaaaaactac
    10921 gccaagtaca acgtgaaaaa ggattacatg gccgtagtct tgatgaccca aattcataat
    10981 aatactcttc gaaaatcaaa ttcaaaccac gtcagcttca ccttgccgta cttaagtaca
    11041 gcctgcggct agcttcctag tttgctcttt gattttcatt gagtataagt attctttctt
    11101 ttaacaagga tagatgaaac gataacaaag agactagcag tttgtgtttg ttagtctttt
    11161 ttcgctaaaa aaggaaccat aatggttcct aaaaactatc attagtaact tgcaccggct
    11221 gtagcgtctg cgtcaccacc gtggcctcca gcatcccctg aatcagaagc gccagaagta
    11281 gcatcggcgt ctccatgatc tccggcagca ggagcaaatg gtccgctacc acccaccaaa
    11341 cgttgaccag tctcttttag gtaccagtca agccatggtt ggaagttaaa gacgatttca
    11401 ttgataccag cgtatgatcc atcaggatag tacattgctt ggtagttgtg agtgttgata
    11461 acacctgcag gagaacctgg aacgatcgta cggacgtatt cttggtttcc gttgcgaagt
    11521 gttccgataa cccactctac gttcttcata cgtgctggtg gaagagaacc atgaacagtc
    11581 gacatacggc tacctgattg aggtggtaca cgtttagcga acatagtgtc tggatcttgg
    11641 tgagcgttgt tgtagtagag gaattggttg ttgtcgtcag cgtatgtcaa ttcaaatggc
    11701 atagctttca agaacatatc aatttggtta actgttagga taccgtggtc caatttgaca
    11761 taggtatcac cagaaacagc accagtgatt gctgcaactt tttctaccca ttctggatcg
    11821 tcagggtcaa cttctgtgat ggttgtagcg attggttttc cacaatctaa gtcttctgat
    11881 tcgattggtt ttggtttttt caatttcgaa acgactccta cgtatttaac aaagttatct
    11941 aagcaagttt caaggaattt aacagtgcct tcgttggtga tatttccgtt gttatcaaaa
    12001 gcttccttag ctttaccaag aaggaattcg ttacctggaa gcgtgtaggc attaacacct
    12061 ggagcatcaa ggattttacg aaggtgaact tgagcacgtg atgttccttg gtcatagtat
    12121 gatgcaccca caatcataac aggcttgttt tcaaatggat gaacttcgta tgaaagccat
    12181 tcaagtacag atttgagtga agctgagata gtgtggttat gctcaggagt agcaatgata
    12241 acaccatctg cacgagtaat tttgttatat aaataacgta attggaaact ttcatcccat
    12301 ttttcatctt ggttaaacat tggaacttcg tcaatttcaa gaacttctaa ttcaaatttg
    12361 agtttgaagt agcgacggat aaattccaag agcttacggt tatatgattg atcgtagttt
    12421 gatccaacaa gtccaacaaa tttcattctt tttggtctcc tatcttacaa attttcccag
    12481 tcaaagtctt cagcatcttt gcgaagtaat tcttgtgcat tacgtaattt ttctgtgatt
    12541 tttacaaaga tacggaagtc atcaaagatg gcatccaatt tcttgataac atcaaggtca
    12601 accaagtcgc cacttgggtt aaatgcttga agagagtgtg agagcaagaa ttcatctgga
    12661 agaacatttg ccttgatttc aggagcattc aagatttgac gaagttgcaa ttgggcacga
    12721 gatgaaccaa gcgtaccgta agaagcacct gtaatcatga ttggtttgtt caaaagtggg
    12781 taaataccat aagacaacca agcaagagcg ctcatcaaaa cagctggaat agagtgatca
    12841 tactcaggag taccgataat aacgccatct gtctcttcga ttttagcagc aatttccaat
    12901 atttcagcag gtacttgctt gtcagctggt ttgttgaaga caggaatggc cttgatttca
    12961 acaagctcaa tttcagcttt gtcagcaaag tgtttttgca tgtattgaag caattgacgg
    13021 tttgtagaac gttttgaatt tgttccaaca atagcaataa gttttaacat gagatttcct
    13081 ttctcttttt acataataca attttaaaat tccattgaaa cagttgtctc tatagagtag
    13141 gaattcctga agaacagctt aggtggcctt ctttatcgat gaggatgact tcgatgccct
    13201 ccaaactttc gacttgccag aggatagaag caggtctttc tccaaagagt cgagtcgtcc
    13261 agatttcgcc atcgactgat ttatcagaga tgattgttag actcgctagt tccgtttcaa
    13321 caggatatcc tgtttgactg tcaaaaatgt gatggtaatc ttgtccatcg acggtcaggt
    13381 gacgttcata aatgcctgaa gtcacgacag atttattgac aacagggatg gtcattaaat
    13441 gatttcccct aggattggct gggtcttgaa tcccgatttg ccatgggtta tcccctcttg
    13501 cctgattttt tccaatggtc aggatattcc ctcccagatt gatcaaggca gaagtcaccc
    13561 cctctttcct aagaaattgg gcaaccttat ccgcactgta tcctttggct aaacaaccta
    13621 gatcgatctt cattcctttc tgttttaaaa acacagtaga agtagaagaa tctaactcga
    13681 taccatgagg attgattaga ggcagcaccg attcaatttc ttgaggctgg gcgaccttgg
    13741 catctgaaaa accgatacgc caggtttgaa ttaagggacc aatgctgata ttgaggtggc
    13801 tagagagcgc taggctatgc tctaacccaa gtgaaatcag ctcaaacagg tctggatgaa
    13861 ccgtgacggg ggctattcct gcttgataat tgatttccat caactcagat tcttgactat
    13921 tggcgttgaa gcggtattca agttctttga gcaagtcaaa ggatttttgg agaaagatat
    13981 cggcttgctc atccactaat gaaatagtga tagtagtccc cattagccgt tcagaatgtg
    14041 aacgaagagt caagctacca actcctttct cttatagaaa a
//
LOCUS       DAVMEF010000039        14008 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMEF010000039 DAVMEF010000000
VERSION     DAVMEF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.039"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            851..2110
                     /locus_tag="VYQ30_000845"
     CDS             851..2110
                     /locus_tag="VYQ30_000845"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1965989.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2126..2677
                     /locus_tag="VYQ30_000846"
     CDS             2126..2677
                     /locus_tag="VYQ30_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1965990.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2670..3947
                     /locus_tag="VYQ30_000847"
     CDS             2670..3947
                     /locus_tag="VYQ30_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1965991.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3963..4823
                     /locus_tag="VYQ30_000848"
     CDS             3963..4823
                     /locus_tag="VYQ30_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1965992.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5203..6006
                     /locus_tag="VYQ30_000849"
                     /pseudo
     CDS             5203..6006
                     /locus_tag="VYQ30_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6197..8488
                     /gene="pcrA"
                     /locus_tag="VYQ30_000850"
     CDS             6197..8488
                     /gene="pcrA"
                     /locus_tag="VYQ30_000850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1965993.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8531..9211
                     /gene="radC"
                     /locus_tag="VYQ30_000851"
     CDS             8531..9211
                     /gene="radC"
                     /locus_tag="VYQ30_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1965994.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9208..9897)
                     /locus_tag="VYQ30_000852"
     CDS             complement(9208..9897)
                     /locus_tag="VYQ30_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1965995.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(9915..10556)
                     /locus_tag="VYQ30_000853"
     CDS             complement(9915..10556)
                     /locus_tag="VYQ30_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1965996.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10639..10854)
                     /locus_tag="VYQ30_000854"
     CDS             complement(10639..10854)
                     /locus_tag="VYQ30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1965997.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(10862..11209)
                     /locus_tag="VYQ30_000855"
     CDS             complement(10862..11209)
                     /locus_tag="VYQ30_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1965998.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11214..12329)
                     /locus_tag="VYQ30_000856"
     CDS             complement(11214..12329)
                     /locus_tag="VYQ30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1965999.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12339..13298)
                     /locus_tag="VYQ30_000857"
     CDS             complement(12339..13298)
                     /locus_tag="VYQ30_000857"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1966000.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13411..13980)
                     /locus_tag="VYQ30_000858"
     CDS             complement(13411..13980)
                     /locus_tag="VYQ30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1966001.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
BASE COUNT         4136 a         2562 c         3070 g         4240 t
ORIGIN      
        1 tagcgattcc caagcctgac tatcgtgagg tagcggatta aaatggtctg gggatagacc
       61 gttttaagtc tgacgctgga aataagaatt gtcagaagaa gggatagcga aatcgtggct
      121 ctacgaacag gaacgtgata ataaggcgta tatagcggat aagagggcct caaactctaa
      181 agtccaaaaa ggtagtcgta acctatatgc gtaaatcacg agagtaattg aattcgtact
      241 aagattttct attttcactg taacctttta acgtccttat atcttgtata cacgaggaaa
      301 gatgtacgac ttatcccgtg aggtctatca ctataaagag aaaacgacag atagaagtga
      361 tcctgagtca cggttatctt taaaatagat tgtctgatag gacggtatgt ataaaacgct
      421 tctgtgaact gagagaaggg ggagaagttc ttgctaaaat ttagttgaac agccgtattc
      481 cgatacttag ataagagatc tagtcttagc tcctactcgg tttcagggga taaaaaaggg
      541 gcaatagcga ttcgagaaag attatactct tcgaaaatct cttcaaatca cgtcaatatc
      601 gccttgccgt acgtgtagga tactgactac gtcagttcca tctacaacct caaaacagtg
      661 ttttgagcaa cctgcggcta gtttcctagt ttgatctttg atttttattg agtattagta
      721 attcagttac taactcgtca actctgattt atccaataaa attgaaaagg atggaaaaaa
      781 ggataaattt atgatatact ttattttgaa gaccttatta gaaatcttga aagagtattg
      841 aaaacttaga atgagaaaaa ttgttatcaa tggtggatta ccactgcaag gtgaaattac
      901 tattagtggt gctaaaaata gtgttgtggc cttaattcca gctattatct tggctgatga
      961 tgtggtgact ttggattgtg ttccagatat ttcggatgta gccagtcttg tcgaaatcat
     1021 ggaattgatg ggagctactg ttaagcgtta tgacgatgtc ttggagattg atccaagagg
     1081 tgttcaaaat attccaatgc cttatggtaa aattaacagt cttcgtgcat cttactattt
     1141 ttatgggagc ctcttaggcc gttttggtga agcgacagtt ggtctaccgg gaggatgtga
     1201 tcttggtcct cgtccgattg acttacacct taaggcgttt gaagctatgg gtgccactgc
     1261 tagctacgag ggagataaca tgaagttatc tgctaaagat acaggacttc atggtgcaag
     1321 tatttacatg gatacggtta gtgtgggagc aacgattaat acgatgattg ctgcggttaa
     1381 agcaaatggt cgtactatta ttgaaaatgc agcccgtgaa cctgagatta ttgatgtagc
     1441 tactctcttg aataatatgg gtgcccatat ccgtggggca ggaactaata tcatcattat
     1501 tgatggtgtt gaaagattac atgggacacg tcatcaggtg attccagacc gcattgaagc
     1561 tggaacatat atatctttag ctgctgcagt tggtaaagga attcgtataa ataatgttct
     1621 ttacgaacac ctggaagggt ttattgctaa gttggaagaa atgggagtga gaatgactgt
     1681 atctgaagac agcatttttg tcgaggaaca gtctaatttg aaagcaatca atattaagac
     1741 agctccttac ccaggctttg caactgattt gcaacaaccg cttacccctc ttttactaag
     1801 agcgaatggt cgtggtacaa ttgtcgatac gatttacgaa aaacgtgtaa atcatgtttt
     1861 tgaactagca aagatggatg cggatatttc gacaacaaat ggtcatattt tgtacacggg
     1921 tggacgtgat ttacgtgggg ccagtgttaa agcgaccgac ttaagagctg gggctgcact
     1981 agtcattgct gggcttatgg ctgaaggtaa aactgaaatt accaatatcg agtttatctt
     2041 acgtggttat tctgatatta tcgaaaaatt acgtaattta ggagcggata ttagacttgt
     2101 tgaggattaa accgtagagg tgtttatgaa tatttggacc aaattagcaa tgttttcttt
     2161 ttttgaaacg gatcgcttgt atttgcgtcc tttctttttt agtgatagtc aggacttccg
     2221 cgagatagct tcaaatccag aaaatcttca atttattttc ccaacgcagg caagtctgga
     2281 agaaagtcaa tatgcactgg ccaattactt tatgaagtcc cctttgggag tgtgggcaat
     2341 ttgtgaccag aaaaatcaac aaatgattgg ttctattaaa tttgagaagt tagatgaaat
     2401 caaaaaagaa gctgagcttg gctatttttt gagaaaagat gcttggtcgc aaggatttat
     2461 gacagaggtt gttagaaaaa tttgtcagct ttcttttgag gaatttggct taaaacaatt
     2521 atctatcatt acccaccttg aaaatgaagc tagccaaaga gttgctctta agtctggatt
     2581 tagtttgttc cgtcagttta agggaagtga tcgttacaca agaaaaatgc gggattatct
     2641 tgaatttcgg tatgtaaaag gagagttcaa tgagtaagca tcaggaaatt ctaagctatt
     2701 tggaggaatt accagtaggt aaaagggtca gtgttcgtag catttcgaat catctaggag
     2761 ttagtgatgg aacagcctat cgggctatta aagaagctga aaaccgtgga attgtggaga
     2821 cccgtcctag aagtggaaca attcgtgtta aatcccagaa agttgctata gagagattaa
     2881 cgtttgctga aattgcagaa gtgacttctt ctgaggttct ggctgggcaa gaaggtttag
     2941 agagagagtt tagtaagttt tcaattggtg ccatgactga acaaaatatc ttgtcttacc
     3001 ttcatgatgg ggggctcttg attgtcggag accgaacccg tattcagttg ctagccttgg
     3061 aaaatgaaaa tgcagttctg gttacagggg gatttcaggt tcatgatgat gtgcttaaac
     3121 tggccaatca aaaagggatt cctgttctaa gaagtaagca tgataccttt accgtcgcga
     3181 ccatgatcaa taaagccttg tcaaatgtcc aaatcaagac tgatattctg acagttgaga
     3241 aactttatcg ccctagtcat gagtatggtt ttctgagaga gacagataca gttaaagatt
     3301 atttggactt ggttcgtaag aatcgtagca gccgtttccc tgttatcaat caacatcagg
     3361 tcgttgttgg cgttgtaacc atgagagacg ctggtgataa atcaccaagc acgacaattg
     3421 ataaggttat gtctcgtagt ctatttttgg ttgggttatc gacaaatatt gccaatgtga
     3481 gtcaacggat gatcgcagaa gactttgaaa tggtaccagt tgttcgaagc aatcaaactt
     3541 tgcttggcgt tgtgacgcga cgagatgtca tggagaagat gagccgttcc caagtttcgg
     3601 ctctaccaac tttttctgag cagattggac aaaagctctc ttatcaccat gatgaagtag
     3661 tcattacagt ggaacccttt atgctagaaa aaaatggagt tttggctaat ggtgtattgg
     3721 cagaaattct gacccacatg acccaagatt tagttgttaa tagtggtcgc aatctcatta
     3781 tcgagcagat gctgatctac tttttgcagg ctgttcagat agatgatata ttgcgcattc
     3841 aggcacggat tattcatcat acgagacggt cagctataat tgattacgat atttatcatg
     3901 gtcaccagat tgtttcaaaa gcaaatgtga ctgttaaaat taattagaaa ctaggagaaa
     3961 agatgataac attaaaatca gctcgtgaaa tcgaagctat ggacaaggct ggtgattttc
     4021 tagcaagtat tcatataggc ttacgtgatt tgattaagcc aggcgtagat atgtgggaag
     4081 ttgaagaata tgtccgccgt cgttgtaaag aagaaaattt ccttccactt cagattgggg
     4141 ttgacggtgc catgatggac tatccttatg ctacctgttg ctctcttaac gatgaagtgg
     4201 ctcacgcttt ccctcgtcat tatatcttga aagatggtga tttgctcaaa gttgatatgg
     4261 ttttgggagg tcccattgct aaatctgacc taaatgtctc aaaattaaac ttcaacaatg
     4321 ttgaacaaat gaaaaaatac actcagagct attctggtgg tttagcagac tcatgttggg
     4381 cttatgctgt tggtacaccg tccgaagaag tcaaaaactt gatggatgta accaaagaag
     4441 ctatgtacaa gggtattgag caagctgttg ttggaaatcg tatcggtgat atcggtgcgg
     4501 ctattcaaga atacgctgaa agtcgtggtt acggtgtagt gcgtgatttg gttggtcatg
     4561 gtgttggccc aactatgcac gaagaaccaa tggttcctaa ctatggtatt gcaggtcgtg
     4621 gactccgtct tcgtgaagga atggtcttaa ccattgaacc aatgattaat acaggcgatt
     4681 gggaaattga tacagatatg aaaactggtt gggcgcataa gaccattgac ggtggattgt
     4741 catgtcagta tgaacaccaa tttgtcatta cgaaagatgg acctgttatc ttgactagcc
     4801 aaggtgaaga aggaacttat taataaaaag tgaaaagact actggaagtt tattttgata
     4861 aaaaatccag tagatctttt cataataaaa cgcattgtat caagtgttag gggctgatat
     4921 catgcgtttt tctgctttta agattttttc caactctgtt tgtaagcgca tcataacaaa
     4981 gggtctagga ttcagggctc tcctcctata tactattagt aaagtaaaac taagggagga
     5041 tattttagtg tcgcagtcta ttgttcctgt agagattcca caatattgtc gttttgattc
     5101 taaaaagaga aatggaattc tgtttaacgt tcgtattgcc aatcttaaat ttattttttt
     5161 atattatact tcctgcgaaa caaaatatgg tatagtagtt ctatgaatga tgaagcaagt
     5221 aaacaactaa ctgatgcacg atttaagcgt cttgttggtg ttcagcgtac cacttttgaa
     5281 gagatgttag ctgtattaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa
     5341 cctaaattaa gcctagaaga ccttcttatg gccactcttc aatagtgcga gaataccgca
     5401 cttatgaaga aattgcggct gattttggtg ttcacgaaag caacttactc cgtcggagcc
     5461 aatgggttga agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagtt
     5521 ctgaggacac ggtaatgatt gatgcgacgg aagtacaaat caatcgccct aaaaaaacaa
     5581 ttagcgaatg attctggtaa aaagaaatgc cacgctatga aggctcaagc gattgtcaca
     5641 agtcaaggga gaattgtttc tttggatatc actgtgaact attgtcatga tatgaagttg
     5701 ttcaaaatga gtcgcagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat
     5761 caagggctca tgaagatata tcctcaagca caaactccac gtaaatccag caaattcaag
     5821 ccgctaacag ttgaagataa aacctataac catgcgctat ctaaggagag aagcaaggtt
     5881 gagaacatct ttgccaaagt agaaacgttt aaaatgtttt caacaaccta tcgaaatcat
     5941 cgtaaacgct ttggattacg aatgaatttg attgctggta ttatcaatca tgaactagga
     6001 ttctagtttt gcaggaagtc tattgaggta ttgagctagt ttatgaaaaa attgggtgaa
     6061 aagtcgagtg ttttagaaac ccacagtgta gtattctagt ttcaatccac tatattttgc
     6121 tactccccgt aaagtttcta ttttccctga tttctgatat aatagaaata ttgacttcaa
     6181 gagtaaggaa gagaagatga acgcattatt aaatggaatg aatgaccgtc aggctgaggc
     6241 ggtgcaaacg acagaaggtc ccttgctaat catggcaggg gctggttctg gaaagactcg
     6301 tgttttgacc caccgtatcg cttatttgat tgatgaaaag ctggtcaatc cttggaatat
     6361 cttggccatt acctttacca acaaggctgc gcgtgagatg aaagagcgtg cttatagcct
     6421 caatccagct actcaggact gtctgattgc gaccttccac tccatgtgtg tgcgtatttt
     6481 gcgtcgcgat gcggaccata ttggctacaa tcgtaatttt acaattgtgg atcctggtga
     6541 acagcgaacg ctcatgaaac gtattctcaa acagttgaac ttggatccta aaaaatggaa
     6601 tgaacgaact attttgggga ccatttccaa tgctaagaat gatttgattg atgatgttgc
     6661 ttatgctgcc caagctggcg atatgtatac gcaaattgtg gcccagtgtt atacaaccta
     6721 tcaaaaagaa cttcgtcagt ctgaatccgt tgactttgat gatttgatta tgctgacctt
     6781 gcgtctcttt gatcaaaatc ctgatgtttt gacctactac cagcaaaaat tccaatacat
     6841 ccacgttgat gagtaccaag ataccaacca cgctcagtac caattggtca aactcttggc
     6901 ttcccgtttt aaaaatatct gtgtggttgg ggatgcggac cagtctatct acggttggcg
     6961 tggtgctgat atgcagaata tcttggactt tgaaaaggat taccccaaag ccaaggttgt
     7021 tttgttggag gaaaattacc gctcaaccaa aaccattctc caagcggcca acgaggttat
     7081 taaaaataat aaaaatcgcc gtcctaaaaa tctctggact caaaacgctg atggggagca
     7141 aatcgtttac tatcgtgccg atgatgagct ggatgaggct gtatttgtag ccagaaccat
     7201 cgatgaactt agtcgcagtc aaaacttcct tcataaggat tttgcagttc tctatcggac
     7261 taatgcccag tcccgtacaa ttgaggaagc cctgctcaag tctaacattc cttataccat
     7321 ggttggtgga accaaattct acagccgtaa ggaaattcgc gatattattg cttatctcaa
     7381 ccttattgct aatttgagtg acaatattag ttttgagcgt attatcaacg agcctaaacg
     7441 tggaattggt ctaggtacag ttgagaaaat ccgtgatttt gcaaatttgc aaaatatgtc
     7501 tatgctggat gcttctgcta atattatgtt gtctggtatc aagggtaagg cagcccaatc
     7561 tatctgggat tttgccaata tgatgcttga tttgcgggag cagctagacc acttaagcat
     7621 tacagagttg gttgagtccg tcctagaaaa aacaggttat gtcgatattc ttaacgccca
     7681 agcgactcta gaaagcaagg cacgggttga aaatatcgaa gagtttcttt ctgttacgaa
     7741 gaactttgat gacaccactg atgtgacaga agaggaaact ggtctggaca aactgagtcg
     7801 tttcttaaat gacttggctt tgattgccga cacagattca ggtagtcagg agacatcaga
     7861 agtgaccttg atgaccctgc atgctgccaa aggtctcgaa tttccagttg tctttttgat
     7921 tgggatggaa gaaaatgtct ttccacttag tcgtgcgact gaagatccag atgaattaga
     7981 agaagagcgc cgtctagcct atgtaggtat cacgcgtgca gagaaaattc tctatctgac
     8041 caatgccaac tcacgcttgc tttttggtcg taccaattat aaccgtccga ctcgttttat
     8101 taacgaaatc agttcagact tgcttgagta tcaaggtctg gctcgtcctg caaatacaag
     8161 ctttaaggca tcatatagca gtggtagtat ttcctttggt caaggtatga gtttggctca
     8221 ggctcttcaa gaccgtaaac gcggtgctgc cccaaaatca atccagtcaa gcggtcttcc
     8281 atttggtcaa tttacagctg gcgcaaaatc agcatctagc gaggcaaatt ggtccattgg
     8341 tgacattgct ctccacaaga aatggggaga gggaactgtt ctggaagttt caggtagcgg
     8401 tgctaggcag gaattgaaaa tcaatttccc agaagtaggt ttgaaaaaac ttttagccag
     8461 tgtggctcca attgagaaaa aaatctaatt ttccatcctt ctcacgaata ataaagtgag
     8521 gaggattttt atgtacagta tttcattcca agaagattca ctattaccaa gagaaaggct
     8581 ggccaaggaa ggagttgaag cgcttagtaa ccaagagttg ctagctattt tactcaggac
     8641 aggaacacgt caagctagcg tttttgaaat tgcccaaaaa gtcttgaaca atctttcaag
     8701 cctaacggat ttgaaaaaaa tgaccctgca ggaattgcag agtttgtctg gtattgggcg
     8761 tgttaaggcc atagaattac aagctatgat tgaactgggg catcgtattc acaaacacga
     8821 gactcttgaa atggaaagta ttctcagcag tcaaaagttg gccaagaaga tgcagcagga
     8881 attaggggat aaaaaacaag agcacctggt ggcactctat ctcaatactc aaaatcaaat
     8941 catccatcag cagaccattt ttatcggttc tgtaactcgt agtatcgctg aaccgcgaga
     9001 gattcttcac tatgcaatca agcatatggc gacttctctt atcttggtcc acaatcatcc
     9061 ttcaggagcg gtagcgccta gccaaaatga tgatcatgtc actaaacttg ttaaagaagc
     9121 ctgcgaactg atggggattg ttctcttgga ccatttgatt gtctctcatt ctaattactt
     9181 tagttatcgt gaaaagacag atttaatcta aagttcatta acgacatagt caaagagttt
     9241 tttatctttg ggacgatttt caaaaagaag ttctggatgc cattggacac cgagaaaggc
     9301 gacatcatcc gtactcatga cagcctcaat gataccatct ttaggatcat gagccacaac
     9361 ttttaaattt ggtgctaagt ccttgatgct ctggtggtgg aaggagttga tatgagagat
     9421 ttctccatag atttcttgga gaacggtatc tggttctgtt accaagcgtt gagttgtgta
     9481 ctcaacagaa gaatcctgcc aatggtcttc gatatcttgg tacaaagttc cacccatggc
     9541 aacgttaaag agttgggtac cacggcagac agagaaaatg ggctttttct gtttaatagc
     9601 ttccttgatg agggccagtt cgaagatatc tctttgaagg tgatagtcat cgctatcaat
     9661 ggttttgggt tcgccataga attttggatc gacattttgc ccacctgtca agatgagctt
     9721 gtcaatcaaa ctgatatagt ggcaggccat ttcttgatca ccaatcggta ggatgatggg
     9781 aatccctcca gcatctttaa cgccttcaac aaagcctttt gctgcgtagc tcatcatgat
     9841 gtcatcatct ggatgagctt tttcgtttcc tgtaatccca ataactggtt ttttcataaa
     9901 atgattttcg ctttctaatc ctcttttcgc atgaagtaga ggagggtttg gagttcactt
     9961 gtcaaatcga catactgaac gaccacgtct tttggtaaat gcagatggac tggtgaaaaa
    10021 ctgagaattc ctttcacacc agcatcaacc aagagattag caacctcttg tgacttgacg
    10081 ctgggaacag ttaggatagc agtcttcaca tcagcatcct tgattttatc cttgatctga
    10141 gaaatcccgt aaatgggaat cccgtcagga gtttgggtac cgacttcagg atggtcgtct
    10201 aggtcaaagg ccatgataat cttcatcttg ttacgttcgt ggaagcggta gtggagaagg
    10261 gcatggccca tatttccaat accaaccagc atgacattgg taatagagtt gtcattgagc
    10321 aaatcggcaa aaaatgtcat tagttttttg acatcatagc caaaaccacg acgaccaagt
    10381 tcaccaaaat aggaaaaatc acgacgtacg gtcgctgaat caataccgat agcctctgca
    10441 atttgcttag agttggcacg ttcaatcttt tctgcatgaa atctcttaaa aattcgatag
    10501 tagagagaga gtctttttgc tgtagctttt ggaatagcaa actgtttatc tttcacaaaa
    10561 tcacaacctt tctattcttc tattttatag aaacattgtg aaaaaatcaa caaaaataag
    10621 aaaaaactaa gaaaaatctt agttttgatg taaaaaatct gcatgagata gaaaacggta
    10681 gaggtctccg accagaccct gataaacttt ttggccccta aaagtcagag aagtcacata
    10741 aagtgtatct ggtaaggtta cacatcctga caaagtcaac atgagagcct catgatcctc
    10801 atacttgaga gtacgctcta catgatagca gtccttatag gtcagttcaa acattttggc
    10861 tctatctttc cgattttgta aagacaccac gttctaccaa gctatccatg aggaagtaga
    10921 atttttcctg atgaatatgg tggtcttctg atttgaaaat atcaactaga cgaaggccaa
    10981 acttgtcagt gatattgatt ttagcccctg taagttcctt gttaatgatg attttgagtt
    11041 ggaagccttc accgctgttt ggcacttttt ccaaaaggcg agtcagttca tagttaccaa
    11101 ccttagtttc aaaaaaggtg ttatctttga gggtgaattt tttaacagaa gggctaagag
    11161 tgtaatcgta acgacaattt tttaactgaa tgattttttc aaatgccata tggctaacct
    11221 ccgataattt cttttaaggt ttttgcgagg gtttgtaggt cttcaacggt attttgtggc
    11281 gacaaactga tgcgaaggga ttccttcaag cgttctgaat ttgcgccata catggcttca
    11341 agaacatggc tggattggac aacgcctgca gtacaggctg agccagtaga gattgaaatt
    11401 ccagctaaat ctagccgaag gagtaagagg tcatttttct gaccaggaaa tccaatattg
    11461 agaacataag ggagatgatg ttttcctcta ttcaggtaat actgaatgcc ctccagctct
    11521 gccagaaagg cagtttctag attttgtaca tgttgaaaat gttcttcttg tttttctagg
    11581 tcttctttta gggctgcaac catgcctacg atggcaggca gattttcagt tcctgcacgt
    11641 tttttctgtt cctggtctcc gccatgtaga taggaatcaa agtccatgct agatgcgtag
    11701 agaaaaccga ttcccttagg accatggaat ttgtgggcag aagcagtgag aaaatcaatg
    11761 cccaattctt ctgaatgaat tgggatttta ccaatagcct gaactgcatc aacatgatag
    11821 gcagcagggt gttgcttgag tatttggcca atttcagcga tgggcagtag gtttcctgtc
    11881 tcattattgg caaacatggt agaaaccaaa atcgtatcgt cacgtaaagc cttttgaatt
    11941 tgctgggctg tgatttcttg attttctggc tggataatgg ttgcttcaaa cccaaagtgt
    12001 tgaaccaagt aatcaattgt ttcaaggaca gcatggtgct cgatggcagt tgtgatgata
    12061 tgttttcctt gttcttggtg acgaagacag tagccaatga tggtagtatt attgccttca
    12121 gtcccaccag aagtgaaaaa gatatgttga ggttttgtcc ttagtaactg ggctagttcc
    12181 tgacgggctt ctcgcaagag tttgccagct tgacgaccat gaccatgaat actagaagga
    12241 tttccgtggg tttcttgcat aaccttggtc atagctgaaa tagcaactgc tgacatagga
    12301 gtcgttgcag cattgtccaa ataaatcaaa gaatcacctt atttcttttt attgtaggca
    12361 aagagtgggc tgactggttt tctttcgtga atacggacga tagcatcacc aattaactca
    12421 ctagcagtga tgtagcatac atttttagga gttttttctt ttgttgctac tgaatcagtc
    12481 acaagaattt ctttaatatt agtattgtca agaagctcag cagctccctc gacgaagaga
    12541 ccgtggctag aaacagcata aatttctgta gctccttcac gttcaacgat tttagaagct
    12601 tcagagaagg tacgtcctgt atttaaaata tcatcaatca agatagcttt cttaccttca
    12661 acatcaccaa taatataacc ttcgttacga gttgcatcgt cttgagggta gtcgataatg
    12721 gcgataggag catcaagata ttcagccagg ctacgcgcac gtttgacacc tgaattttta
    12781 gggctaacga caacaacatc tgaaccaagc aatcctttat cgcagtaatg ttttgcgaat
    12841 aggggaacag tgaaaagatt atccactgga atatcaaaga aaccttgaac ctgaacggca
    12901 tgcaaatcaa gagtcaggat acgatcaact ccagccttaa ccagcatatt ggcaactagt
    12961 tttgctgtaa gtggctcacg aggacaagca atgcggtctt gacgtgcata gccaaaatat
    13021 ggaaggacaa cgttgatact gtgggcactt gcacgcacac aagcatcgac catgattaac
    13081 aattccatta ggtggttgtt gacagggaaa cttgttgatt ggatgatgta aacatcataa
    13141 ccacggacac tttcttcgat atttacttgg atttctccgt ctgaaaattg acgtgatgat
    13201 agttttccaa gtgggacacc aacagcttgg gcaatttttt gtgcaatctc ttggttagag
    13261 ttgagtgcga aaagtttcat gttttttcta tctgacatta tagaccgtcc tctgtaaact
    13321 ttataaatcc tagttatatt taccttacat atatgaactg ggatttgtgt atttttatct
    13381 tttctatttt accaaaaaat ggagattatt tcagctattt ttcatacttt tgacaaatcg
    13441 aaccaatttt gaaggagctt tttggtagga aatctgattt ttctctaaaa attgtcggaa
    13501 atcctgtttg ccttgctcat gattttccac ttcaagctcc aattcgtaat ctgttatatc
    13561 aaagtatcgg ctctgatcca gtgccatgag accaatagct gttttcattt catagcgaag
    13621 cgttgttaga caaccaagaa cctgccagtt cttactttgg ataccatgtt tcgccaattc
    13681 atccagtact agcccttgag gaagttcttc cttactcaga tagttctcag catcttttag
    13741 ttgcaatttt tggttgtatt ccatgtttcc aacactctgc gggactttga gtgtcaactc
    13801 agcccagtct tcaaaggttc gaatgcgcat agcgactttc ttttctcgca gttcaaaatc
    13861 aggcgtgtcg atgtagtaat ttgtttgaag aacaggagtg acacctgtga actggtcttt
    13921 tagacgattg tattcatctt ttttcaatag tgttttcaat tcaatttcta aatgtttcat
    13981 ttttcttacc tttttttatc gttgaaag
//
LOCUS       DAVMEF010000040        13946 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMEF010000040 DAVMEF010000000
VERSION     DAVMEF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.040"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(280..1887)
                     /locus_tag="VYQ30_000859"
     CDS             complement(280..1887)
                     /locus_tag="VYQ30_000859"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1966002.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2277..2864)
                     /locus_tag="VYQ30_000860"
     CDS             complement(2277..2864)
                     /locus_tag="VYQ30_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1966003.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2992..3303)
                     /locus_tag="VYQ30_000861"
                     /pseudo
     CDS             complement(2992..3303)
                     /locus_tag="VYQ30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3201..3617)
                     /locus_tag="VYQ30_000862"
     CDS             complement(3201..3617)
                     /locus_tag="VYQ30_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966004.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            complement(3645..4271)
                     /locus_tag="VYQ30_000863"
     CDS             complement(3645..4271)
                     /locus_tag="VYQ30_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1966005.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4285..4848)
                     /locus_tag="VYQ30_000864"
     CDS             complement(4285..4848)
                     /locus_tag="VYQ30_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1966006.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5013..5247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5301..5804)
                     /locus_tag="VYQ30_000865"
     CDS             complement(5301..5804)
                     /locus_tag="VYQ30_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1966007.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(5997..6827)
                     /locus_tag="VYQ30_000866"
     CDS             complement(5997..6827)
                     /locus_tag="VYQ30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1966008.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRLLV
                     LIAIFSVFLLYLSEIHFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6812..8494)
                     /locus_tag="VYQ30_000867"
     CDS             complement(6812..8494)
                     /locus_tag="VYQ30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966009.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8596..8853)
                     /locus_tag="VYQ30_000868"
     CDS             complement(8596..8853)
                     /locus_tag="VYQ30_000868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966010.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(8860..9408)
                     /locus_tag="VYQ30_000869"
     CDS             complement(8860..9408)
                     /locus_tag="VYQ30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1966011.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9423..10271)
                     /locus_tag="VYQ30_000870"
     CDS             complement(9423..10271)
                     /locus_tag="VYQ30_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1966012.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10459..11808
                     /gene="trkA"
                     /locus_tag="VYQ30_000871"
     CDS             10459..11808
                     /gene="trkA"
                     /locus_tag="VYQ30_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1966013.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11812..13251
                     /locus_tag="VYQ30_000872"
     CDS             11812..13251
                     /locus_tag="VYQ30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1966014.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13378..13602)
                     /locus_tag="VYQ30_000873"
     CDS             complement(13378..13602)
                     /locus_tag="VYQ30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1966015.1"
                     /translation="MIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQ
                     IVATRGMEYIHLTKNPSKSLQFVLEHYQAV"
     gene            complement(13614..13835)
                     /locus_tag="VYQ30_000874"
     CDS             complement(13614..13835)
                     /locus_tag="VYQ30_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIBC"
                     /protein_id="HEV1966016.1"
                     /translation="MEVVLFSYCLNTRYLLQVIDIASSNDIILEEDTSEIMPGELDEI
                     KSKELKVLVLCAGSGTSAQLANAINKGLT"
BASE COUNT         4078 a         3159 c         2597 g         4112 t
ORIGIN      
        1 ttgacttttc tgatagagat gttcatatct tatttcaatt cactatacaa tccagttcct
       61 aaaactttgc tagaaattga tttggctttc cgaattaatt tgttcagatt ttatttcatt
      121 tcaatatact gataaatcca ttcactagca cagggcaaga gggagtcgct attaatttca
      181 acggtagcag gtacatacag aggactccta cctcagatat actgcaatat cattgtatgc
      241 aaaaaacctc tgagattttt ctcaaaggtt ctgattttgc taattgctgt tctcaaccgc
      301 tgcagtaaca aaggcagtgt agagttcttc tggtcggttt ggacggcttg acagttcagg
      361 gtgatactga caagctacaa agaatttatt ttcaggaatt tccacgattt ctaccaaacg
      421 attgtctgga gaaactcctg aaaagacaaa gcctgctgcc tcaaactgct cacggaaggc
      481 gttattaaac tcataacggt gacggtgacg gcgttgcacc acttcttgat tgtgataagc
      541 agcagcagcc ttagagccac gtttcaactt agacggataa agtcccaaac gaagggttcc
      601 acccatatcc tcaatatcaa tctgatcacg catgatatca atgatagggt attttgtttc
      661 tggtgcaagc tctgcagaat tggcaccttc aagacctaaa acgtgacgag caaactcgat
      721 acatgtcaac tgcattccca agcagactcc caacattgga acatcatttt cacgcgcata
      781 gcggatggct tggattttcc cttctgtacc acgttgacca aaaccacctg gtacgatgat
      841 cccgtccgca tcagacaaga gttctgctac attctctgct gtcacatcat tggcattgac
      901 ccaattgatt ttaacttctg catcattgac atagccagag tgtttcaagg cttcgaccac
      961 tgagatatag gcatcttgca actccacata cttaccaaca agggaaatct taacttgttt
     1021 cttgaggttc atgaccttat ccaccatggc tgaccattct gtcatagccg ctgctggtgc
     1081 gtctaatttc aaatgatcac aaacaatttg gtccatccct tgtgcctgca agttcagtgg
     1141 aatttggtaa aggtgttcaa catccaacga ttcgataacg gcttctggtg ccacatcaca
     1201 gaactgggcc agtttatttt taattccttg accagctggc tcttctgtac gaataaccaa
     1261 catatttggt tggattccca agccacgcaa ttctttgaca gagtgttggg ttggtttggt
     1321 tttcatttca ccagcagcct tgaggtaagg aagcaaggtt gtatggatat acatgacatt
     1381 atccgcaccc acatctgcct tcatctgacg aagagcctct aggaatggca aggactcgat
     1441 atctcctact gttccaccaa cctctgtgat aatgacatca gagtcggtcg ttagagcggc
     1501 acgcttgatt ttttctttca aagcatctgt gatatgagga atgacttgaa cagttgcccc
     1561 aaggtattct ccacggcgtt ctttacgaag aacttcactg taaattttcc cagttgtcac
     1621 gttggaatat ttgttgagat tgatatcgat gaaacgttca tagtgaccca agtccaaatc
     1681 tgtctcagct ccgtcatctg tcacaaaaac ttccccgtgc tggtaaggac tcatggttcc
     1741 cggatcaata ttgatataag ggtcaaactt ttgaatggtt actttgagac cacgattttt
     1801 caagagacgg cctagactcg ctgccacaat ccctttccca atagacgata ccacaccacc
     1861 agttacaaaa atatatttcg tagacataga ttcctctttc taaaatgctc aaggtcttgt
     1921 taactacgag gggatataga aaacaagacc ctactaataa gaataatctg ggacgactgc
     1981 accagattca caactaatac tcttcgaaaa tctcttcaaa ccgcgtcaac gtcgccttgc
     2041 cgtagatatg tgttcctgac tttgtcagtc ttatctacaa cctcaaaaca gtgttttgag
     2101 cagcctacgg ctagtttcct agcttgctct ttgattttca ttgagtataa aatacaagaa
     2161 gataacttct tgaaaaaaca aaaatagctc cctaagaact agggagcccc gacctctaaa
     2221 agaggtgccc aaacaatatg atacctaaaa ataggataat tgtcaatagc taactcttat
     2281 tcctcgctgg tttcagcatc gtcatctgaa aactcgtcgt cgtcttcgtt gagatccaca
     2341 tcttctccca agtcatctgg agcgatttcg ttgatttctg catcataagc ttccacttca
     2401 tttttttcat catctggatt ttcatcatcg tatgaaagag ctggatctgc ttcgtatgca
     2461 tcttcgtctt ctggatcatc tgcattgtag tcaatggcat ctgaatcacc atccataaag
     2521 gcattgacac gttttttctt agcttttggt gctacttcat cgtcgtcatt ttcttcaaga
     2581 gcgatgattt cttcgtcgat ttcgtccaca ccataccatg aacgaagacc ccatttgttg
     2641 tccccaagtg agatgaagct accgtcaaag ttcaactctg tgtagaacaa aggcaaagct
     2701 tcgcggatat cgctgtttga tgttccaagg tagttttgaa tttcgtttac aagatcgcta
     2761 aaatgcatct catgatcgcg accacgaagt tccaagatag cacgcgctac ctcaatcata
     2821 gatagttcac ttttttcttg cccagcaaat acttctaatt ccaaagcgtt tctcctcatt
     2881 tatactacta tcgccagagc gaacagactc tgacctcatt ttatcattta ctctttattt
     2941 tacgataatt ttgcggaata gtcaaaggtt aagggggaga aagtggcagg attagactaa
     3001 ttccaatata aaactccttc ctttttctgt tgctccattt tccacaaatc caagcgactt
     3061 gaaacacctc ctagaagcat gattgtaggt gtagatttcc ttgactctca attctttcca
     3121 tccttttact cgagccaatt caatcaaagc acttagaatc ttttttccaa gtcctcgatg
     3181 ttggtaagcg gaattcccaa tcacaatggg gagattatcc tgagatagtg taatatcccc
     3241 aattggaaac cattctccct tctccttgac ttcaatccaa aaaagctcac catgccgatc
     3301 caaagaggta agatatgatt ttttaaatca aaaacagagt aaatctttct ttcctcgcaa
     3361 gcttgcgcat agagatgata tagttcatca ccaccatctc tatcccactc agcagaaatc
     3421 gtatcccgac ctgccaataa agcctgataa gccctgtgat gcccatctgt aatcagcaaa
     3481 caatctccaa aggcaagaat actgattgga tcgacttggt ttgtttctgc cgactggtaa
     3541 agcatctgaa tatcttgcaa cttcttttct gataaatata gttgagtcgg atgaagatct
     3601 gctatattga ctttcatttc tttctcctca agggaattcg atactcactt ctgtttgcct
     3661 ttaaatcgcc attggaagcg gagcttgtca tagaagggaa actcgataaa caggactccc
     3721 aagcccacac agagactggc aaggacgtct gatgggtaat gaactcccag atagactctt
     3781 gataccagca cactgactag gtagaggcca aggacgattt gtacgatttt tctccagact
     3841 ggatttttaa ttcgttgact gagaatgaca atcagagtgc ctaccatcaa ggttacagcc
     3901 agagaatgcc cacttgggaa ggaaaatccc ttctcctcca caagatgtaa gatagctggt
     3961 cgtgggcgct ggtagatatt tttaaaggtc acgattaaaa gacctgccaa agccagattt
     4021 cccagcatga agaaactttc tatcttccat cgcttacgat aaaagacaaa agctgtaatg
     4081 acaacccaag tgataatcac tgggatatca atcagacgtg tgagggctcg aaaaagaata
     4141 gtcaaataat ctggtaagtc tcctcgaatg gcagtctgaa tcgattggtc aaaattgacc
     4201 agcgtttcag ggtaaaattt gaccatgtag ccaagaataa cgaaaagtaa aagggcaaaa
     4261 ctgcccttca ttaaaaatgt ttgtttatct ctcataatgt tttaaggttg gtttcaagag
     4321 aacatacaac aaccagaatg aaacggaaaa gataacacct tcaatcaagt taaaaggtaa
     4381 taccatggtc attaggtagt tggaaagtcc caaaattttt ccaatatcaa agttagcaaa
     4441 cttagcgtac aaaggaacag cataaacata gttgagaacc aacatggcca aggttaaacc
     4501 aacagttcca gctagagagc ctagtaggaa acgaagggtt gtccgttcct ttttccaaat
     4561 caaagcaaat acgatgacaa aaactcccaa agctacgata ttcatcggca aaccaatgta
     4621 agtattcact ccttggctgt taagaagcaa tttcaatagt gagcgaagca agagcactcc
     4681 tagagcagca ggcaaatcca tgaccaccag acccacaagg actggcaaga tactaaattc
     4741 gatcttgagg aaagatgccg ctggtaaaag cggaaagtca aagtacatca gcacaaatga
     4801 gatggctgat agaattgcaa tggtcgaaag tcgacgtgtg tttgtcataa caggttcctc
     4861 caattttcta taaaatcaga agaagttgga aaggattcct ctatctattc tcacttttta
     4921 tatcccaaaa gttccctctt actctattaa agaaaaacaa agcaagtggt tacaatccgg
     4981 ctataaatct atcaaaacag acaaagctat tctttcgtct tctcccatcc agactatact
     5041 gtcggttgtg gaatctcacc acatcagctt gcgctcgcgg acttctttaa agagaaggaa
     5101 atagagattt ttcttttaga ctaggcgaca agctgtaggc ataacttctg gttaggcaaa
     5161 gcttgtcaac aaagtataaa aggaaaagat catttcctag ttaaagtcac cgccggtcgg
     5221 gaatcacacc ctgccctgaa gactctttta tcataacaaa aaacgcttgc aagcgcaagc
     5281 attttgttca ttttcatttt ttatttcaat ttatccactt cataggtatg gacgagttca
     5341 agacctgcaa agttctgctg gcgaagagcc tcatagacaa tcatgcagac ggtattagac
     5401 acattgagac tgcggacatg ttcatcattc atgggaatac gaagagcctt ctcaggatgt
     5461 tctcgcataa aatcttcagg taagcccttg tcttcacgtc caaagagaaa ataatggtcc
     5521 tcgtcagtcg ataaatccac ctcagaatag attttttccg cgaatttcga aatcagatag
     5581 agttttccct tcatctgaga catgaaatct tctaagctct cgtaaaaata aatctctagc
     5641 ttatcccagt aatccaatcc agcccgcttc atcttgcggt catcaatagg aaagcccata
     5701 ggcttgatga tgtggagggg agaattggtc gcagcgcaag tacgcgcgat attgcctgta
     5761 ttttgtggaa tttgaggttc aaataataca atgtgatttg tcatgacttg cttcctttca
     5821 ccattgcaaa aaaatagcca cactgcccgg agtcaagctc agcaaacagc gtggttaagg
     5881 catcgttaac ttacctcaca acaggtttga agtaaatcag cgaaactact ttcttagtat
     5941 aacactttca gaatcattgt caatagaaat gacttgattt tttcaatttt ttcaagctat
     6001 ttccaagggt tgtaaaatcg tccctgattc tgcaagataa gtagtaaact aactactaaa
     6061 aacaaggttg ccaagagcaa ggtaatatag tctccttttt tcaaggcctg ataactatac
     6121 catgtgcgtt ttttctcttt cccaaagcgg cgaagctcca tggcagtcgc aatggtatca
     6181 atgcgttcta gcgagctaaa aatcaaggga gtaataatga gcagattgcc tttgattcgt
     6241 tgcataagag aagctttctt ggataattcc atcccacgcg cttcctgaga catcttgatg
     6301 gtaaagaatt cttcctgcaa atctggaata tagcgcaagg ttagactgac agaataggca
     6361 atcttgtaag gcacaccaat ttgatttaaa ctggaagcaa actgactagg gtgggttgtc
     6421 atcaaaaaga taatagccag aggaatagtg caaaggtact taatagccaa atttagcaga
     6481 taaaagagct cttgactggt cagagtgtag gcaccgattc cctgccaaat cacacttctc
     6541 tctccgtaaa gtccaacccc atactcggga gaaaagagat agaccatcaa aacgtttaaa
     6601 acggcaaata ccgtcgcaaa aacggctaca aaggaaacat ctttaaaatg gatttctgac
     6661 aaatagagga gaaagaccga aaagatggca atcagaacaa gcagtctggt atcatagcta
     6721 atcatggccg ccaatgacac gagaatgaaa aagaggagtt tcccagcgcc tgacaagcga
     6781 tgaatcacag tatctctatg ctggtaaccg attaatttag cttgcatcct tctctccttt
     6841 ctttgtaaaa tgccgttaaa tccagtggat ccacatctag tttcttagcc aagttgaaga
     6901 tggaggtttc ttttagattg gcttttacta acagctcagg atcgctcaac agactggctg
     6961 gaacagtatc ggcaatcaat tctccatcca ccatgacaag gacccggtct gaataatcca
     7021 gcatcaattg catatcatgg gtaatcatga caatggtatg ccctttttga tgtaactctt
     7081 cgagaaattc cataatctca gtatagttct tctgatcttg acctgcagtc ggttcatcta
     7141 ggagaataat ttcagctcct aagaccaaaa ttgaagcaat ggtgacacgt tttttctgac
     7201 caaatgacag ggcagaaata ggccaattac ggaattcata aagtccacag attttcaagg
     7261 tttcatagac tctcgtttca atttccttct cattcacacc tcgcaaacgg agccctagag
     7321 ccacctcatc aaaaatcata ttggttgaaa tcatttgatt aggattttgt agcacatagc
     7381 ctactcgttc cgcccgctct gcaacagaat cgccttttat atcctgtttt tcccaaagat
     7441 agcgtccttc cgtctgaata aagctactta tagccttggc tagagttgat ttccctgctc
     7501 cattttttcc gacaatagca atcttttcac cctttttaat atctaaatgt agggatttta
     7561 aaatcggtct atcattataa gaaaaagata cttcctctag tataaagagt gactgcaatg
     7621 ctggggtttc ttttaccagt tccgtctgta actggatttg cccttttgag atagacaagt
     7681 tatccagatc tgctaattgt tcttccttga ctaagtccac acctaattga cggagagtcg
     7741 ttagataaag gggttctcga attccatttt gagtcaataa atcagtcgca agcaactggt
     7801 cagggctccc attaaaaagg atacgaccat cgtttatcaa gacaatccga tccacagggc
     7861 gatgcagaac gtcctccaaa cggtgctcga taataagagt cgtcgtcccc tcttccttat
     7921 gaatctggtc aatcaattcg ataatatcct gacctgactt gggatctaga ttggcgagtg
     7981 gctcatcaaa caagagaatc ggactttcat caatcaagac accagccaga ctgactcgct
     8041 gcttttgtcc acctgacaaa tcctgaggac gctgatccag taaaggaaga aggtccagct
     8101 tttcagccca tttataaaca cgacctttca tctcatctag ggctgtcaca tcattttcca
     8161 gagcaaacgc caaatcttct gccacagaca agccaataaa ctgcccatct gtatcctgca
     8221 aaactgtgct aaccagatga gacttatcat agatgctcat atcaaaggct gtttgaccct
     8281 ttatcaaaaa ttctccatat gtctgaccct tgtaaatatt gggaataatc ccattcaaac
     8341 actgacccaa ggtagattta cctgatccag atggtccaac aattaagact ttctctccct
     8401 tgtaaatggt caagtctatc ccttgcaagg tcggttcttg ttgtgtttca taccggaaag
     8461 agaaatcctt ccactcaatt atagcttctt tcatcttact ctcttcattc gcttcttaga
     8521 cttctatttt atcataaatc aagcccttct tgcagtctct tctcttaaaa tcttagcgcc
     8581 aaaaagattc ctatcctagc ttacttgcct aactaatcta taaacatcga aaaagactag
     8641 ttgcccagcc ttccccatca ttttatactc ttcgaaaatc tcttcaaacc acgtcagctt
     8701 cgccttgccg taggtatggt tactgacttc gtcagtttca tctacaacct caaaaccatg
     8761 ttttgagctg acttcgtcag ttctatccac aatttcaaaa cactgttttg agcaacctgc
     8821 ggctaacttc ctagtttgct ctttgatttt cattgagtat tagtcctttt tcaaacttcc
     8881 tgcacgagtt tgggttcctg cataggcaag taagagaaga gttcctgcaa tagctacaga
     8941 tacaccattg gcaattcccg caacaatccc ttgtgcaaat actttttctg ccgcttcttg
     9001 ataaatcaca acatctccaa gtggtgccaa gacaccccaa acaagggcat ttgcaagtag
     9061 ttgaatgagg ttaaaaataa gaatatcttt ccagtcaaaa acaccattga tcacgcgaac
     9121 gtactttcta aaaagtccca caactaaacc aaagagtccg ctagcgataa tccaagtcca
     9181 ccatagacca taaccagcaa gagagtcctt gattgcatga ccaatcaacc cgacaagcaa
     9241 accgataatc ggtccaaaaa taatagaaag tagcgcttgt accgcatact gaagctggat
     9301 gcttgtattt ggaacagggg ttggaatgtt gatcatcccg atgacgacaa agagggcagc
     9361 gccaattccg acagcaacaa cttgtttaat tgtaaattta atttccatac tattctccta
     9421 ttttatcctt ctattttctt tatttcaatg gtccaagatg aaccgacacc tacattatag
     9481 gccttggcaa aggaaccttg gttgatagcc aaacctaaac gatagagaga gttgatgtaa
     9541 aggatgggtt gcccaattct cacatctgca aatgatttgc catagacaac ctgattttga
     9601 tagaccagca tatcagcatg atagatggtc acttcaaaac gatcaccaaa ttctggttcc
     9661 agcttgtaaa attcttcccg tgtgatagag gtccaaagcg aaccgaaacg cacatccaga
     9721 atatcaatgg ctcccttcac cagatgatct tctatgatgg tcgctacgac tggaagctct
     9781 acaatctgtt ccacactgag ctctggccct acttcctcaa aagtaatgtg accactggcc
     9841 agtttagcac cagtataggc atagacatca cgaccgtgga aggtataaga atgctctgtg
     9901 ttttgacgcc tattggccac ctcagaaatc tcacgaatgg ctacaatgcc aacgtgtttc
     9961 ttgataaagg aaagcgtccc attatctggc gtgacaatgt attgattttt tgcagtcttg
    10021 gcaactacac tcttacgttt cgaaccgaca cctggatcga caaccgatac aaacgtcgtt
    10081 ccctcaggcc agtaatccac cgtctgaaag agacgatagc tcccctcaaa aatattataa
    10141 ggcgtgatat cgtgcgtcaa gtgatggatt tttaaggttg gagactcttc taaagccact
    10201 ccaatcatag ccgataccgc accatcaacc agaccaaagt ctgattgtaa taccagtaaa
    10261 ttattattca ttttatctcc ttgtatatcc tttatcatac catatttcag agaaagtcgc
    10321 taatagctat ttcaattggt tagggtatag ttttacctta tagcgaattt tcactaaaag
    10381 ctcttgttat tagctagtag gtgcattttt tcttgaaaaa gggtatgata gttacatcat
    10441 gaaaaagtag gttttattat gaaaattatc cttgtcggag ggggaaaagt tggttttgcc
    10501 ctctgtcgct ccttggttgc agaaaagcat gatgttttgc tgattgagca agacgaagct
    10561 gttctcaatc atattgtcag tcgctttgat atcattggta tccttggtaa cggggccgat
    10621 tttgccattc ttgagcaagc cagcgtccaa gattgtgata tctttatcgc cctgactgag
    10681 cacgatgaag tcaacatgat tgcagcagtt ctagccaaga aaatgggagc taaagaaact
    10741 atcgttcggg tgcggaaccc tgaatattct aactcttatt tcaaggaaaa gaatattctc
    10801 ggtttttctc ttatcgttaa tcctgagctc ttggctgccc gcgctatcgc gaatatcatt
    10861 gacttcccca acgccctgtc tgtcgaacgc tttgctggtg gacgcgttag cctcatggaa
    10921 tttgtcgtca agtccaccag cggtctttgc caaatgccca tttctgattt tcgtaaaaaa
    10981 tttggtaatg tcattgtctg tgcgatagag agggatcatc aaattatcat tccaagcggt
    11041 gacatgactg tacaggataa agatagaatc tttgtcactg gtaaccgtgt cgatatgata
    11101 ctcttccata attattttaa atcacgcgcc gtgaagagcc ttctcatcgt tggagcaggt
    11161 agaattgcct attatctact tggtattctc aaagatagtc gtatcgatac aaaagtcatt
    11221 gaaatcaatc ctgaaatcgc cagcttcttt agcgagaaat tcccaaatct ctacatcgtt
    11281 caaggagatg gaaccgcaaa agatatcctg ctggaagaaa gtgctcaaca ctatgatgcc
    11341 gttgcgactc taacaggagt cgatgaggaa aatctgatta catctatgtt ccttgacagg
    11401 gtaggtgtac agaaaaatat tactaaggtc aatcgtacca gtctcctcga gattatcaat
    11461 gcgcctgatt tttcaagtat catcacacct aaaagcatcg ctgtagatac gattatgcac
    11521 tttattcgtg gtcgagttaa tgcccagtat tcagaccttc aagccatgca ccatctagcc
    11581 aatggccaaa tcgaaaccct gcaattccat atcaaggaag ccaataaaat gactgccaaa
    11641 cctctttctc aactgaaatt gaaaaaaggg gttcttattg cagccattat tcgaaagggc
    11701 aagactattt tcccaactgg ggaggatatg ttggaagttg gagacaagct cctagtaaca
    11761 accttgttgc caaacatcac caagatttat gacttgattg cgaggtaaga aatgaataaa
    11821 agtatgattc gttacctcct ttcaaagtta cttttgattg aagctgttct cctcttggtt
    11881 cctgtggcta tcgctgtcta ttaccgtgaa tcgagccaag tctttacagc cctcttttcg
    11941 accataggga ttctcgtatt gttaggcggt tcagggagtt tacagaagcc aaaaaatcaa
    12001 cggatttatg ccaaggaggg agtcttgatc gttgccctct gttggatcct ttggtctttc
    12061 tttggtggtc tcccctttgt cttttctggg caaatcccca gccttattga tgcctttttt
    12121 gagattagtt ccggctttac gactactgga gcaagtattt tgaacgacgt ttcagttctc
    12181 agtcgttccc tcctcttctg gcgaagtttt acccacttga ttggagggat gggagtgctt
    12241 gtttttgcac ttgctattat ggacaatgcc aaaaatagcc acctagaggt gatgaaggct
    12301 gaggttccag gtcctgtttt tggcaaggtt gtatccaaac taaaaaacac tgcccagatt
    12361 ctctatctcc tttatctagc tctcttctcc ctctttgtca tcatctatta tctagctggt
    12421 atgcctctct acgatagttt tgtcattgct atggggacag cgggaactgg aggctttacc
    12481 gtctataacg acggaattgc ccactatggc agctcactga ttacctatct ggtcagtatc
    12541 ggagttctgg tttttggagt aaatttcaac ctctactact acctcatgct ccgtcgcatc
    12601 aaggccttct ttggagatga agagcttagg gcttacttgg tcattgtact ggtttctaca
    12661 ggcttgatta gcctcaacac cctctacctc tacccagggt tttcaaagag ctttgaaatg
    12721 accttcttcc aggtttccaa catcattaca acaactggtt ttggatatgg agatattacc
    12781 aactggcccc tcttctccca gtttatcctc cttttcctca tggcaatcgg tggctctgct
    12841 ggatcaactg caggtggact caagattatt cgaggcctca tcctttcaaa aattgccaaa
    12901 aaccaaattt tgtcaatcct atcgccccac cgtgttttga ccctccatgt taataaaacg
    12961 gtgattgaca aagataccca gcataagatt ctcaagtact ttgtcatcta tgctatgatt
    13021 ttgctagcgc ttatctttat tgtcagccta gatagcaatg attttctggt cgtgaccagc
    13081 gctgtcttta gctgtttcaa taatatcggg cctattctag gaaccacttc tagtttctca
    13141 atctttagtc ccatctcaaa aattctccta tcctttgcaa tgattgcagg gcgcttggag
    13201 atttacccaa tcctccttct ctttatgaag agaacttggt ctaagagata aaatacaaaa
    13261 tccctctttt tacaattgta aaaagaggga ttcttatatt aaaaaagggg gaacgttttt
    13321 aaatccaagc ctgtctgtaa gcaaagtcct tactcaaata aaagatggaa aaacttacta
    13381 cacagcttgg taatgctcca atacaaattg taaggattta cttggattct ttgttaaatg
    13441 aatatattcc attcctctgg tagcaactat ctgaatacct aatctttctg catccacctt
    13501 catctctcta taataactcc gaacttgtgg ggccagaata attaaatcat aaacacccat
    13561 aatatcataa tgagctccgt acgctcctga attcgcaatc actctaacct ctgttaagtt
    13621 agccccttgt taattgcatt ggctaattgc gcacttgttc cagaccctgc acaaagaacc
    13681 agtactttca actccttact ttttatttca tctaactcac caggcattat ttcacttgta
    13741 tcctcctcta aaataatatc atttgagctt gcaatatcta taacctgtag tagatatctc
    13801 gtatttagac aatatgaaaa caagacgact tccatatagg aaaccgcctt ctcgctatgt
    13861 tgagtgattt atattaaaat aacttttctt ctagctgcat tttattatta taaaaacatt
    13921 catcataacc cccagaactt aaataa
//
LOCUS       DAVMEF010000041        13902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMEF010000041 DAVMEF010000000
VERSION     DAVMEF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.041"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..191
                     /locus_tag="VYQ30_000875"
     CDS             <1..191
                     /locus_tag="VYQ30_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966017.1"
                     /translation="KEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRI
                     FIALNIKKERTKFVLSRA"
     gene            complement(484..966)
                     /locus_tag="VYQ30_000876"
     CDS             complement(484..966)
                     /locus_tag="VYQ30_000876"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1966018.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(1061..2545)
                     /locus_tag="VYQ30_000877"
     CDS             complement(1061..2545)
                     /locus_tag="VYQ30_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1966019.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            2698..4308
                     /locus_tag="VYQ30_000878"
     CDS             2698..4308
                     /locus_tag="VYQ30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1966020.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKSVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(4452..5849)
                     /locus_tag="VYQ30_000879"
                     /pseudo
     CDS             complement(4452..5849)
                     /locus_tag="VYQ30_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            6024..7469
                     /locus_tag="VYQ30_000880"
     CDS             6024..7469
                     /locus_tag="VYQ30_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1966021.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLSDIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            7471..8202
                     /gene="cps4B"
                     /locus_tag="VYQ30_000881"
     CDS             7471..8202
                     /gene="cps4B"
                     /locus_tag="VYQ30_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1966022.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            8211..8903
                     /gene="cpsC"
                     /locus_tag="VYQ30_000882"
     CDS             8211..8903
                     /gene="cpsC"
                     /locus_tag="VYQ30_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1966023.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            8913..9602
                     /locus_tag="VYQ30_000883"
     CDS             8913..9602
                     /locus_tag="VYQ30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1966024.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            9617..10978
                     /locus_tag="VYQ30_000884"
     CDS             9617..10978
                     /locus_tag="VYQ30_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1966025.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            10984..11727
                     /locus_tag="VYQ30_000885"
     CDS             10984..11727
                     /locus_tag="VYQ30_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV1966026.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            11724..12548
                     /locus_tag="VYQ30_000886"
     CDS             11724..12548
                     /locus_tag="VYQ30_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV1966027.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            12550..13428
                     /locus_tag="VYQ30_000887"
     CDS             12550..13428
                     /locus_tag="VYQ30_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966028.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            13429..>13902
                     /locus_tag="VYQ30_000888"
     CDS             13429..>13902
                     /locus_tag="VYQ30_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1966029.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRI"
BASE COUNT         4153 a         2395 c         3063 g         4291 t
ORIGIN      
        1 acaaagagaa aatcgtcaac gcccttcaac taccctattc aaacgccaaa ttggaagcga
       61 ccaataatct catcaaactt atcaagcgca atgcctttgg ttttcggaac tttgaaaact
      121 tcaaaaaacg gatttttatc gctctgaata tcaaaaaaga aaggacaaaa tttgtccttt
      181 ctcgagctta gctttttttc aacccactac agttgacaaa gagccaaaaa aactggttcc
      241 ttttgaggta cgacgggcat gtcgtgtagt agagatatac tattctagtt tcaatctact
      301 atagtagctc agaagtcggt acttaaacgt gctatatcaa aaccagtcct tgaaaaacgt
      361 ggactggttt cgtgtttgga ttattacctt gaacggcatg cgttaaaagt tagttgaacc
      421 gccgtatgcc gaacggcacg tacggtggtg tgagaggggc tagagattat cccctactcg
      481 attttaaatc acatgacgtt caaaggcatc atctgaaatc ccttgttcca agatgagttt
      541 tgcccattct ttagcagaga agaggctgtg gtccttgtag tttccgcaag attcgatggt
      601 tgtccctggg acatcttccc aagtagtagt ttcagcgatt tccttgagcg aatccttgat
      661 aacagctgcg attttagcac tggtgtgacg tccccacata atcatgtgga agcctgtgcg
      721 gcaaccaaat ggtgaacagt caatcatgcc gtcaatgcgg gtacggatga gtttggctaa
      781 gaggtgctcg atagtgtgaa ggccggcagt agggatagag tcttcgtttg gttgcaccaa
      841 gcgaatatca taattggaga tgatgtctcc ttttggtcct gtttcttccc caatcaagcg
      901 aacatagggt gctttgacaa tggtgtggtc aagttcaaaa ctttcgacaa taacttcttt
      961 tgacatggta aatcctttca gttttcttct ctcattatat cataaagctt gttcctgaga
     1021 cagagagaaa acctctccga ggctggagag gttgaaatct ttacttacga tataagcggt
     1081 cgtattggta gtatgggtca aaggttacgt tgatacccag tttacgaagg acattcttgt
     1141 cttcatcagt caagatgatg gttgagtggg cttcgcttcc tttgaggttg ccgagttctt
     1201 ccatagcgcg ggcagcatca ggattttctg tagctgtgat agcaagtgca atcaggattt
     1261 catttgaatg aaggcgtgga ttgcggctac cgagatgatc gattttaaga ccttggattg
     1321 gcttaacaac ttcaggctcg attagtttta cttctttagc gatgtcagct gattttttga
     1381 tggcgttgat caaggcagcg gctgtaggcc caaagagttc tgagttctta ccagtgacga
     1441 tttccccatt tggcaattca aaggctaggg ctggtccacc agtttcttct gctttttggc
     1501 gcgcaacgac agcaacctta cggtctgcag gtgagatacc gaggtcgttc atgagcaact
     1561 caattttctt gacggcagct tcgccaactt tttcagcttt gaagtcaaga actgtttgat
     1621 agtaacggcg gatgatttct tgtttagaag cttcgacagc agcctcgtca tctgtaatag
     1681 cgaaaccaac catgttgaca cccatatctg tcggtgaagc gtatggtgat tttccgagaa
     1741 tacgttccaa catgcgcttg agcactggga agatttcgat atcacggttg tagttgacag
     1801 tggtttctcc ataggtttga agatggaagg ggtcaatcat gttgacatca tcaaggtcag
     1861 ctgtggcagc ttcataagcc aagttaactg gatgatgaag gggaagattc caaacaggga
     1921 aggtttcaaa tttagcgtag ccagacttga tgccattgat ttggtcgtgg tacatattgg
     1981 acatacacgt tgccaatttt ccagaaccgg gtccaggagc ggttacgaca atcaagttgc
     2041 gactggtttt gatgtagtcg tttttgccca tgccttctgg ggaaatgatg tgatccatat
     2101 ccgtcggata tcctttgatt ggataatgaa gataagaatc aattccgttt ttctcaagtt
     2161 ggttgcggaa ggcatctgca gcgagttggc cagcgtattg tgtaatgaca acggaaccaa
     2221 caaaaatccc taattcattg aatttgtcaa tcaaacgaag aacttcttgg tcataagaaa
     2281 tgcccaagtc gccacgtgct ttggaatgtt caatgttgct agcattaatg gcaatcacaa
     2341 cctcaacctg ctctttcaat tcttgcaaga gcttgatttt gttgtcaggt tcataaccag
     2401 gaaggacacg agcagcgtgg aaatcttcta acattttacc gccaaactct aagtagagct
     2461 tgccgtcaaa ttggttaatg cgttccaaaa tatggtcgcg ttgtaaattc aaatattgtt
     2521 cagaactaaa agcttgtttt ttcatttttt tacctctgac ctctattata ataaaaaatt
     2581 ggaagttagg aaactacgga gctaaaaaag aaattaaaaa gattaagcaa acgcttgcac
     2641 aaaattttaa aaagtgttat catatactat agattatgaa aataatgagg taaacagatg
     2701 caagaaaaat ggtggcataa tgccgtagtc tatcaagtct atccaaagag ttttatggat
     2761 agtaatggag atggagttgg tgatttgcca ggtattacca gtaagttgga ctatctagct
     2821 aagttaggaa tcacagcgat ttggctttct cccgtttatg acagccctat ggatgataat
     2881 ggctacgata ttgctgatta tcaagcgatt gcaacgattt ttggaaccat ggaggatatg
     2941 gatcaactga ttgcggaagc taagaagcgt gacattcgta tcatcatgga cttggtggtt
     3001 aatcatacct cagatgaaca tgcttggttt gtcgaagcct gtgaaaatac tgatagccct
     3061 gagcgagact actatatctg gcgcgatgaa cccaatgacc tagattctat ctttagtggg
     3121 tctgcttggg aatacgatga aaagtcaggt caatactatc tccacttttt cagcaagaaa
     3181 cagccggatc tcaactggga aaatgaaaaa cttcgccaga aaatttatga gatgatgaac
     3241 ttctggattg ataaaggtat tggtggtttc cgtatggatg ttattgacat gattggcaaa
     3301 attcctgacg agaaggtagt caataatggt cctatgctcc atccctatct caaggaaatg
     3361 aatcaggcga cctttggagc taaggatctc ttgacagtag gggagacttg gggagcaacg
     3421 ccagagattg ccaagctcta ctctgatcca aaggggcaag aattgtctat ggtcttccag
     3481 tttgaacata tcggtcttca gtatcaggaa ggtcaaccta aatggcacta tcaaaaagag
     3541 ctgaatatcg ctaagttaaa agaaatcttc aacaaatggc agacagagtt aggagttgag
     3601 gacggctgga attccctctt ctggaacaac catgacctcc ctcgtattgt ctcaatctgg
     3661 ggaaatgacc aagaataccg cgaaaaatct gccaaagcct ttgcaatctt acttcatctc
     3721 atgagaggaa ctccttatat ctaccaaggt gaggagattg ggatgaccaa ctatccgttt
     3781 gaaacactgg atcaagtaga agatattgaa tccctcaact atgcgcgtga ggctcttgaa
     3841 aaaagtgttc cgattgaaga aatcatggac agtatccgtg ttattggacg tgacaatgcc
     3901 cgtaccccta tgcaatggga cgagagcaaa aacgctggtt tctcaacagg tcaaccttgg
     3961 ttggcggtta atccaaatta cgagatgatc aatgtccaag aagcgctggc aaatccagat
     4021 tctattttct atacctatca gaaactggtc caaattcgca aggagaatag ctggctaatt
     4081 cgagctgact ttgaattgct tgatacggct gataaggtct ttgcttatat acgtaaggat
     4141 ggcgaccgtc gcttcctagt tgtggctaac ttgtccaatg aagagcaaga cttgacagta
     4201 gaaggaaaag tcaaatctgt cttgattgaa aacaccctag ctcaagaagt ctttgaaaaa
     4261 caaatcttag ttccatggga tgctttctgt gtggaaatga ctgactagaa tgagcaaacc
     4321 tcaagttttt gaagcttgag gttttttact atagtggatt gaaactagaa tagtgcacct
     4381 ctgcttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctattctt
     4441 atttcatttt gctatacttg tagttgagga gaattaagct cctcactagt aaactcttca
     4501 taatcctttt tatttttatg aagatattgt ttgaaagatg tgagtttcca cggatgggtt
     4561 tgtggaggga tatacttgcg tctttctttt tttgttctgg ttcttgttca aagtttttcg
     4621 aatagagttc atgatttagt agctcctttg tgtgatagat tttgtcagcg atattgaggt
     4681 agatgtctcc gtcaaatgct tttataacta atgctttcgt ctttctgatg aaatagactt
     4741 cttttccttg ttcggtaggg atatagtaac gattttggaa tcggatatgg tgtccactat
     4801 cgacgactct ctccgccagt ctagccagaa tgagattcct ttcagagggg ttaggaacct
     4861 cctcgaagac agagagtttt gtcttgtttc caaactgttc attaaaggtt tgaatgtagg
     4921 aaagcaggaa ggtattggct tcttccaagg tatgaatatt gttttgttcc agttcgatag
     4981 gcaggcgaga ttgtagtgtc tgattgagtc tttcgactct ccctttagct tgagggatag
     5041 aggttgtctc aaggagaatc cctagttggt gacaggcgta tccaaactgt gtatgggtat
     5101 cgtcctccat tttcttagag ttggaggctt gataggtaaa gaccgttctc ttatctgttt
     5161 taatttgaag gggaatgccg tgattggcta agatttgttc gaggacatga tagtaagcat
     5221 tcaagttctc ttgtttatca aaataagcgc ctaggatatt gccagaagca tcatcaatgg
     5281 ctaagtgtaa gttagatgtt tgggctccaa actaggcatg aggactggca tccatttgaa
     5341 tgagttcacc agcaaatttc tttctgggtc tactaggatg tactttttta gggtcttcaa
     5401 ggaagttttc agccgtcggt aagattggat tgtctagggg ttgattcagg ttcagtttag
     5461 cttgttttct tactctcttc tttgtctttc tatgggactt aggcgacagg atatttttct
     5521 tatagagtat ttttctaaca gtagtatcag agagctgaat tccttcttct tcagctagca
     5581 attcacagaa atgaaggaca tttggtttat atgtttcata ggagaggtat ttttttagga
     5641 gacgttcttt gatttcatca gggattgcat gttttggttt tcgatttctg tttccgtgtc
     5701 tgaaggcttc tttgtccttt cagttgatag gctagtagca gacgattgat ttgtctttca
     5761 gaaagattga gctcgacaca ggctcgtttc tttgttttct ttccttgggc tatagctttt
     5821 atcacaagat attttttcgt ttcattcata ttcagttgga tccttttcat atgactattc
     5881 taccaaatgg gacattttca cgttcgattt actaaagaca ttatcacatt cgaattacac
     5941 aagatgcaga tagtaaaaaa aatgtagaca ttaccgtaaa aaagtgatat aattgtatga
     6001 tgttcaaggt ataggtgtta atcatgagta gacgttttaa aaaatcacgt tcacagaaag
     6061 tgaagcgaag tgttaatatc gttttgctga ctatttattt attgttagtt tgttttttat
     6121 tgttcttaat ctttaagtac aatatccttg cttttagata tcttaaccta gtggtaactg
     6181 cgttagtcct actagttgcc ttggtagggc tactcttgat tatctataaa aaagctgaaa
     6241 agtttactat ttttctgttg gtgttctcta tccttgtcag ctctgtgtcg ctctttgcag
     6301 tacagcagtt tgtgggactg accaatcgtt taaatgcgac ttctaattac tcagaatatt
     6361 cgatcagtgt tgctgtttta gcagatagtg agattgagaa tgttacgcaa ctgatgagtg
     6421 tgacagcacc gactgggact gataatgaaa acattcaaaa actactatct gatatcaaat
     6481 caagtcagaa taccgatttg acggtcaatc agagttcgtc ttacttggca gcttataaga
     6541 gtttgattgc aggagagact aaggccattg tcttaaatag tgtctttgaa aatatcatcg
     6601 aatcggagca tccagattac gcatcgaaga taaaaaagat ttataccaag ggattcacta
     6661 aaaaagtaga agctcctaag acgtctaagg atcagtcttt caatatctat gttagtggaa
     6721 ttgatactta tggtccgatt agttcggtgt cgcgttcaga tgtcaatatc ctgatgactg
     6781 tcaatcgaga taccaagaaa attctcttga ccacaacgcc acgtgatgct tatgtaccaa
     6841 tcgcagatgg tgggaataat caaaaagata aattgaccca tgcgggcatc tatggagttg
     6901 attcgtccat ccacacttta gaaaatctct atggagtgga tatcaattac tatgtgcgat
     6961 tgaacttcac ttctttcttg aaattgatcg atttgttggg tggggtagat gtttataatg
     7021 accaagattt tacatctcta cacgggaagt tccatttccc agttggaaat gtccacttag
     7081 attctgagca ggctctggga tttgttcgtg agcgctattc cctagcggat ggtgatcatg
     7141 accgcgggcg caatcaacaa aaggtgattg cggccatcct tcaaaaatta acttcgagcg
     7201 aagcgctgaa aaattatagt aggatcattg atagcttgca agattctatc caaacaaaca
     7261 tgccacttga gaccatgata aacttggtca atgctcagtt agaaagtggt ggaacgtaca
     7321 aagtgaattc gcaagacttg aagggtatgg gacggacgga tcttccttcc tatgcgatgc
     7381 cagatagtaa cctctatatg atggaaatta acgacagtag ccttgcatct gtcaaaactg
     7441 ctattcagga tgtgttggag ggcagatgaa atgattgata ttcattcgca catcgtcttt
     7501 gatgtagatg atggtcccaa gtcaagagag gaaagcaagg ctctcttggc agaatcctac
     7561 aggcaggggg tgcgaaccat tgtctctacc tctcaccgtc gcaagggcat gtttgaaact
     7621 ccggaagaga agatagcaga aaactttctt caggttcggg aaatagctaa ggaagtggcg
     7681 agtgacttgg tcattgctta cggggctgaa atttactaca catcagatgt tttggataag
     7741 ctggaaaaaa atcggattcc gaccctcaat aatagtcgtt atgccttgat agagtttagt
     7801 atgaacaccc cttatcgcga tattcatagt gccttgagta aaatattgat gttgggaatt
     7861 actccagtca ttgcccacat tgagcgctat gatgcacttg aaaataatga aaaacgcgtt
     7921 cgagagctga tcaatatggg ctgttacacg caagtaaata gttcacatgt cctcaaatcc
     7981 aaactttttg gagaacgtta taaattcatg aaaaaaagag ctcagtattt tttggagcgt
     8041 gacttggttc atatcattgc aagtgatatg cataatgtgg acagcagacc tccccatatg
     8101 gcagaagcat atgaccttgt ttcccaaaaa tacggagaag cgaaggctca ggaacttttt
     8161 atagataatc ctcgaaaaat tgtaatggat caactaattt aggagaaatg atgaaagaac
     8221 aaaacacgat agaaatcgat gtatttcaat tacttaaaac cttgtggaaa cacaagctaa
     8281 taattttatt agtggcactt gtgacagggg cgggagcttt tgcatatagc acttttattg
     8341 ttaagccaga atatacaagc accacgcgta tttacgtagt caaccgtaat caaggagata
     8401 agccgggact gacgaatcag gacttgcagg caggaactta tctggtaaaa gactaccgcg
     8461 aaattatcct ttcgcaagat gtattggaaa aggtagcgac aaatttgaaa ttggatattc
     8521 cagcaaaaac gttaactagc aaagtgcaag tgactgtacc agccgacact cgtatcgtct
     8581 caatctctgt caaggataaa caaccagagg aagccagtcg cattgctaat tctctacgag
     8641 aagttgctgc agaaaagatc atcgctgtaa cgcgagtatc tgatgtaacg acgcttgaag
     8701 aagcgcgacc agctatgact ccctcttctc caaatgttcg acgcaacacc ttagttggtt
     8761 ttcttggagg agccgccgta acagtaatta ctgttctttt gattgagttg ctcgataccc
     8821 gtgtaaaacg tcctgaagat gttgaagatg tactgcaaat gccacttcta ggagtcgttc
     8881 cagattttga taaaatgaaa taggaggaag ttatgccaac gttagaaatc tcacaggcga
     8941 aattagaact tgtgaaaaag gcagaggaat attataatgc tttgtgcacg aacctacagt
     9001 taagtggaga tgatttgaaa gtattttcta tcacttctgt gaaagcagga gaaggaaaaa
     9061 caacgacttc caccaatatc gcttgggctt ttgcgcgtgc aggttacaaa acgcttctga
     9121 ttgatgcaga catgcgtaac tccgttatgt caggtgtctt taaatcaagg gaaaggatta
     9181 ctggactaac agaatttcta tcagggacaa cagacctgtc acaggggctt tgcgatacca
     9241 atattgagaa tctctttgtg attcaggctg gctctgtgtc accaaatccg acagcccttc
     9301 ttcaaagtaa gaatttcagt acaatgcttg aaaccttgcg taaatatttt gactatatcg
     9361 tcgtagacac tgcccctgtc ggtgtcgtga ttgatgcggc tatcattatg cagaaatgtg
     9421 atgcttctat tttagtgacg aaggcaggtg aaacaaaacg acgggatctt caaaaagcga
     9481 aagaacagtt ggaacaaact gggaagtcgt gtttaggagt tgtgttgaat aaactcgata
     9541 cttcagtaga gaaatacggt tcttatggga gttatgggaa ttacaggaaa cagaaaaaat
     9601 aggttgaggg atagaaatga gtgaaaaact agcaaagcct tcagtagcca tagtccagag
     9661 ttttcttgtt attttattga cttatctact tagcgctgtg agagaaacag agattgtttc
     9721 aacaacagct attgtacttt atatcctcca ctattttgtc ttttatatca gtgattatgg
     9781 acggaatttc tttaaaaggg gatatttgat tgaacttgtc cagacattga aatatatcct
     9841 attctttgcg ctagcgatta gtatttctaa ttttttctta gaagatcgat ttagtatttc
     9901 cagaagaggc atgatttact tcctcttatt acatgttttc ttagtctatg tgctaaaccc
     9961 atttatcaag tggtattgga agcgggctta tcccaacttt aaaggaagta agaagattct
    10021 cctacttaca gcaacttctc gtgttgaaaa ggtactggat agactaatag aatcagatga
    10081 tgttgttggg gagttggtag ccgtcagtgt tttagataaa ccagattttc agcatgatga
    10141 tttaaaggtt gtagcagagg gggagatagt aaattttgcg actcatgagg tggtcgatga
    10201 agtctttatc aatcttccaa gtgaaaaata caatattgga gagcttgtct ctcagtttga
    10261 aacgatggga attgatgtaa cagtcaatct aaatgctttc gattgggcac gtaacaagca
    10321 aatttgtgag atggcaggac taaatgttgt gactttttct acaacatttt ataagactag
    10381 ccatgtgatt gctaagcggg ttattgatat tgtcggttcc ctggtaggtt tgatattatg
    10441 tggtctagtc agtattgtgc tggttccttt gattcgaaag gatgggggtt ctgctttttt
    10501 tgctcagacg cgtataggaa aaaatggccg ccattttact ttttataagt ttcgctctat
    10561 gtgtgtggat gctgaggaga aaaaaagaga actcatggaa caaaatacca tgcagggtgg
    10621 aatgtttaag gtggacgatg atcctcgtat cacgaaaatt ggtcatttta tacggaagac
    10681 gagcttggac gagctaccac agttttataa tgttctaaag ggagatatga gtttggtagg
    10741 tacacgacca ccaacagtgg acgagtatga gcactatacc ccagaacaaa aacgtcgcct
    10801 aagttttaaa cctggcataa caggtttatg gcaggtcagc ggacgaagtg aaatcaaaaa
    10861 tttcgatgaa gttgtcaaat tagatgtggc ctatattgat ggttggacaa tctggaaaga
    10921 tattgaaatt ttattgaaga cagttaaggt tgtattgatg aaggatggag cgaaatagtt
    10981 catatgaatg agagaattca aatattagga attactatag atccattaac gatgaaagaa
    11041 acagtggatg ctgttgaaca gtatgtttta aagaagcacc ctttgcactt gatgggagtt
    11101 aacgcggata aaattaatca gtgctatgaa gatgagaaaa tcaaaaaaat cgttaatgaa
    11161 tcaggaataa ttaatgccga cggtgcttca gtagttttag cgagtaagtt tttaggaaca
    11221 ccagttcctg agcgagttgc tggaatagac ttgatgaaac atttactaga gttgtctaat
    11281 gaaaaaggat actcggtcta cttttttgga gcaaaagaag acgttttaca agatatgtta
    11341 aaaatttttg aagaaagata tccagctcta aatgttgttg gctatagaaa tggatatttt
    11401 tcgcctgaag atgaaaaaca tattcaggaa gatattaaag agaaaaaacc agattttgtg
    11461 tttgtaggaa ttacttctcc taagaaagaa tatattatcc aatcattcat ggataatgga
    11521 attaatgctg tgtttatggg ggttggtgga agttttgatg tactatcagg gcatatcaaa
    11581 cgagctccat tatggatgca aaatgctcac ctagagtggt tgtttcgtgt ggcaaatgag
    11641 cctaaacgtc tctttaagcg ttattttgta gggaatgttt catttataag aaaagtttta
    11701 agagcgaaaa ggggatataa actttgaacc aaacagaaat agttaaattg attcagaaag
    11761 ttgaattgga tgctataaaa gagtttcaaa aaatttgtaa agagaacaat atagatttct
    11821 tccttcgtgg ggggagtgta cttggggcag tcaaatatga tggctttatt ccatgggatg
    11881 atgacatgga tattgctgtg cctcgtgaag gctacgataa acttccaggt atctttaagg
    11941 atagaattat cgcaggcaaa tatcaggttc ttgcttatca atattgcgat actttacatt
    12001 gctactttcc tcgcttattc cttttagaag atgaaagaaa acgtttggga ttgccacgaa
    12061 ataccaattt aggtttgcat ttgattgata tcattccttt ggatggtgcc ccaaatcatt
    12121 catttttaag aaagctttac ttcggtaaag tatactggta tcgtttttta gcaagtttag
    12181 gaactaccta tgttggagac catgtggata tgcattccgc taagcaaaaa ctaatcattg
    12241 gtttctttaa aaagctagga tttgcaaaac tattccctca aaattatgtg tacagacgct
    12301 tggataatct ctataaaaag tatgattgga aaaagcagaa gtatgctggg acaattaatg
    12361 catctctgtt tgccaaagaa gtcatgccag tagagatttg gggagaagga gtagagaagc
    12421 cttttgagga tgccttcttt aaagttccca cggagtatga ccgctatctg aaaagacttt
    12481 acggagaaaa ctatcttcac gaagagccta gtgatgatga aaagaaatcg catttaggag
    12541 gacaataatt tgttttgtta tattattttg cattacaaag tcttagagga aactatttct
    12601 tgtgttaaat caataaaaga aggcaattcc aatacaaagc agatcgttat tattgataat
    12661 ttttctaata acggtacggg tgaaaaacta caagagctgt atgagtcaga ttcagagatt
    12721 gatgtcttga ttaaccatga aaatgctggt ttcgctcgag gtaataatgt agcatatcag
    12781 tttgctaagg aaaagtacaa acctgatttt atggttatca tgaataatga tattgagata
    12841 gaaacagaag agtttgaaaa aatcgtgaca gatatctatc ggaaggaaaa attccatttg
    12901 ttaggaccag atatcttttc gacgacgtat cagcttcacc aaaacccaaa acggttgacg
    12961 cattatactt atgaagaggt taaggctctc aatgaaaaat ttaagaaagg gagccaagtt
    13021 agtctagcat taaaaattaa atgttggttg aagtctagta aagttcttcg gacagcaatc
    13081 tatcaaaata ggcgtaaaaa gaaatcagta gactatagaa aacaggtaga aaacccaatt
    13141 cttcatggtt cgtttattgt atattctaga gattttattg agaaagagga gtatgctttt
    13201 aatcccaata ccttcttcta ttatgaaaca gagatattag attatgaagc tgagttaaaa
    13261 ggatataaga gaatttatac accgaagatt aaggtcttgc accatcaaaa tgttgcaacc
    13321 aatcaagtct atacaaactt agtagaaaaa accttgtttt caaacaaatg caactttgaa
    13381 tccactagtt attttttgaa gttgatgaaa gaaaacgagg gtgtgtaaat gacttattta
    13441 tttttactct gcctgacctt attcttatta actttcttct atttttttgc tttcaatcaa
    13501 gatttgatag ctccgccagt tgtcatgtct gttatgtttc tcattagttc tgtatttgcc
    13561 cttattaatg tgcaaaactg gaatattgag tatagtggtt tagcttatct tttgattatt
    13621 agtggcatta ttgttttttc aatgcctctc ttggctctta attcgcctat tctaaatacc
    13681 aagattaaag tgacggatcg gctcattgat atccaattct ggaaaatagc tcttactatt
    13741 atagttgacc tcattattct atatctttat aggagagaga ttcataatct tgcacttagc
    13801 catggttata cgggttcaaa ttttcagtgg ttctttagaa atgctaccag ttatgaaggt
    13861 gagctaacag tgcgaacttc gattcgggtc ctcattcgta tc
//
LOCUS       DAVMEF010000042        13881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMEF010000042 DAVMEF010000000
VERSION     DAVMEF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.042"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            520..972
                     /locus_tag="VYQ30_000889"
                     /pseudo
     CDS             520..972
                     /locus_tag="VYQ30_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            991..2328
                     /locus_tag="VYQ30_000890"
     CDS             991..2328
                     /locus_tag="VYQ30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1966030.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEII
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDNDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2407..3291
                     /locus_tag="VYQ30_000891"
     CDS             2407..3291
                     /locus_tag="VYQ30_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1966031.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3291..4124
                     /locus_tag="VYQ30_000892"
     CDS             3291..4124
                     /locus_tag="VYQ30_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1966032.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4142..6235
                     /gene="nanB"
                     /locus_tag="VYQ30_000893"
     CDS             4142..6235
                     /gene="nanB"
                     /locus_tag="VYQ30_000893"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1966033.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTNGELIYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAVTELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6247..7350
                     /locus_tag="VYQ30_000894"
     CDS             6247..7350
                     /locus_tag="VYQ30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1966034.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7524..8222
                     /locus_tag="VYQ30_000895"
     CDS             7524..8222
                     /locus_tag="VYQ30_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1966035.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8241..9758
                     /locus_tag="VYQ30_000896"
     CDS             8241..9758
                     /locus_tag="VYQ30_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1966036.1"
                     /translation="MFKVLQKVGKAFMLPIVILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9883..11211
                     /locus_tag="VYQ30_000897"
     CDS             9883..11211
                     /locus_tag="VYQ30_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966037.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11463..12329
                     /locus_tag="VYQ30_000898"
     CDS             11463..12329
                     /locus_tag="VYQ30_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1966038.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12345..13184
                     /locus_tag="VYQ30_000899"
     CDS             12345..13184
                     /locus_tag="VYQ30_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1966039.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13456..>13881
                     /locus_tag="VYQ30_000900"
     CDS             13456..>13881
                     /locus_tag="VYQ30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1966040.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRK"
BASE COUNT         4446 a         2324 c         2841 g         4270 t
ORIGIN      
        1 tcttaaaagt ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag
       61 tgctagaata atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa
      121 gtccccaaat agattcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg
      181 aatatcaatt tcatctataa ggaaggtagc taattgaact aacttattta ttctgtttgt
      241 cgctagaaaa atcagacctc cttgtgaaga ttgaggagat acttaatgaa aatcaaagaa
      301 gaaactagca agctagtagc agattgccca aaacaccgct ttgaggttgt agataagact
      361 gacctatata atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtataggc
      421 tagagagtag tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact
      481 tggataggaa aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc
      541 cgtttaggaa cttatgtggg agtaaatcca cattttgcaa cattaataga ttttctagaa
      601 aaaacaggac tagaaaattt aacagaaggt tcgattgcta tcgatggtaa tcgattgttt
      661 gggaattgct ttacttatct agcagatggt caagcagggg ctttctttga aacacaccaa
      721 aaatatttgg atattcattt agttttggaa aacgaagaag ccatggctgt tacatcgccg
      781 gaaaatgtaa gcgttaccca ataatatgat gaagagaaag atattgaatt atacacaggg
      841 aaagtggaac agttggttca gttgagagct ggcgaatgcc tcatcacttt tccagaagat
      901 ttacatcaac ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt
      961 gcgatttctt aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta
     1021 gctggaattg cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca
     1081 actagtactg aaccaccaac agaattatct ggtgagatta taatgtggca ctcctttact
     1141 caaggacccc gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca
     1201 aaaacgaaaa tcaagattga aacattttct tggaatgact tctatactaa atggactaca
     1261 ggtttagcaa atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa
     1321 atggtcaact cagatgcttt ggttccgcta aatgattcta tcaagcgtat tggacaagat
     1381 aaatttaacg aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct
     1441 ctttattcac atgcacaagt catgtgggtt agaacagatt tgttaaaaga acataatatt
     1501 gaggttccta aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga
     1561 gtttatggct tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac
     1621 ttctacgtac gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca
     1681 agccaacttg ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct
     1741 caagattctt tgaactttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca
     1801 gcatttgact ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg
     1861 attgattcga ttgatgctta tcctattcca aaaatcaaag agtctgataa cgaccaagga
     1921 attgaaacct caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct
     1981 aaagcattct tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact
     2041 ccagtaggta tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat
     2101 gaaactcgta agaagtttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt
     2161 actgctattg gttatgaaaa tggaccaagt gtacaagctg gtatgttgac taaccaacac
     2221 attattgaac aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca
     2281 aaagaagcag aaaaacaatt aaacgattta tttgaggctg ttcagtagat gtaaaagact
     2341 agaaaatagg tgggctagtg agctgaaaag ctctagccca atcttgtaaa agaagggaga
     2401 aggagaatgg ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt
     2461 atcgtaggat tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat
     2521 aagcatttga ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta
     2581 tcagatccca acttctttaa tgcgttcttt aattcaatta agtggaccgt tttctcatta
     2641 gttggtcaag ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc
     2701 aagaaattat ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc
     2761 ttctcttggc agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa
     2821 ttaggtttaa tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt
     2881 ttggtgttta tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct
     2941 ttgcaaacag taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg
     3001 caggtgttca agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt
     3061 ttgagaactg tatggatctt taataacttt gacattatct acctcattac tggtggtgga
     3121 ccagccaatg ctacaacgac gcttccaatt tttgcttaca acctgggctg gggaactaaa
     3181 ttgttgggtc gtgcttcagc agttacagta ttgctcttta tcttcttggt ggcgatttgc
     3241 tttatctact ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga
     3301 aatccagtat ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg
     3361 cagttttccc attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc
     3421 agtatccaac acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca
     3481 acgatttgca cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc
     3541 ttattgcgat tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat
     3601 tgggagcaat catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag
     3661 caattcccta ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga
     3721 tgatggttta tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc
     3781 aaacagttcc aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta
     3841 cgttttataa agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata
     3901 catttatcaa tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa
     3961 agatgacagt agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag
     4021 atatgatggc agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc
     4081 aaaataagat tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa
     4141 aatgaataaa agaggtcttt attcaaaact aggaatttct gttgtaggca ttagtctttt
     4201 aatgggagtc cccactttga ttcatgcgaa tgaattaaac tatggtcaac tgtccatatc
     4261 tcctattttt caaggaggtt catatcaact gaacaataag agtatagata tcagcccttt
     4321 gttattagat aaattgtctg gagagagtca gacagtagta atgaaattta aagcagataa
     4381 accaaactct cttcaagctt tgtttggcct atctaatagt aaagcaggct ttaaaaataa
     4441 ttacttttca attttcatga gagattctgg tgagataggt gtagaaataa gagacgccca
     4501 aaagggaata aattatttat tttccagacc agcttcatta tggggaaaac ataaaggaca
     4561 ggcagttgaa aatacactag tatttgtatc tgattctaaa gataaaacat acacaatgta
     4621 tgttaatgga atagaagtgt tctctgaaac agttgataca tttttgccaa tttcaaatat
     4681 aaatggtata gataaggcaa cactaggagc tgttaatcgt gaaggcaagg aacattacct
     4741 cgcaaaagga agtattgatg aaatcagtct atttaacaaa gcaattagtg atcaggaagt
     4801 ttcaaatatt cccttgtcaa atccatttca gttaattttc caatcaggag attctactca
     4861 agctaactat tttagaatac cgacactata tacattaagt agtggaagag ttctatcaag
     4921 tattgatgca cgttatggtg ggactcatga ttctaaaagt aagattaata ttgccacttc
     4981 ttatagtgat gataatggga aaacgtggag tgagccaatt tttgctatga agtttaatga
     5041 ctatgaggag cagttagttt actggccacg agataataaa ttaaagaata gtcaaattag
     5101 tggaagtgct tcattcatag attcatccat tgttgaagat aaaaaatctg ggaaaacgat
     5161 attactagct gatgttatgc ctgcgggtat tggaaataat aatgcaaata aagccgactc
     5221 aggttttaaa gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga
     5281 tttccgttat acagttagag aaaatggtgt cgtttatgat gaaacaacta ataaacctac
     5341 aaattatact ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga
     5401 acaatattcg gttgattttg atagtggctc tttaagagaa aggcataatg gaaaacaggt
     5461 tcctatgaat gttttctata aagattcgtt atttaaagtg actcctacta attatatagc
     5521 aatgacaact agtcagaata gaggagagag ttgggaacaa tttaagttgt tgcctccgtt
     5581 cttaggagaa aaacataatg gaacttactt gtgtcccgga caaggtttag cattaaaatc
     5641 aagtaacaga ttgatttttg caacatatac taatggagaa ctaatctatc tcatttctga
     5701 tgatagtggt caaacatgga agaaatcctc agcttcaatt ccgtttgaaa atgcaacagc
     5761 agaagcacaa atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac
     5821 aggtaagata gcttatatga ctagtagaga ttctggagaa acatggtcgg aagtttcgta
     5881 tattgatgga atccaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca
     5941 attaattgat ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa
     6001 gggaggccaa ttagttgtcg gtttagtcaa taaagaagat gatagtattg attggaaata
     6061 ccactatgat attgatttgc cttcgtatgg ttatgcctat tctgcggtta cagaattgcc
     6121 aaatcatcac ataggtgtgc tgtttgaaaa atatgattcg tggtcgagaa atgaattgca
     6181 tttaagcaat gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag
     6241 aaaaacatgg ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc
     6301 tacatgcaaa agaatgatgg agcagagatt actcttctct atgatccaga taatgcagag
     6361 gcgattgcag aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc
     6421 gatgaggtag attgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt
     6481 aaggctgcac agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa
     6541 gattgtcgcg agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat
     6601 attatgaatt tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc
     6661 ggagacgttc tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta
     6721 tcatggaaaa aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg
     6781 gattgcgttc aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat
     6841 gtggcccatg aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa
     6901 ttttctaata agcgttttgc cttgttagaa tggggttcag cttatcgttg gggtgaacat
     6961 tatgtcttaa tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga
     7021 actcttaagc tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat
     7081 gatgatcgga ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa
     7141 ccaggtaaac gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg
     7201 catgagatta tggaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa
     7261 gctgccctag aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc
     7321 aaagtaaaat tgtcagaaat tgtaaaataa attttggtat tctcctattt ataggtcgac
     7381 ttgctcctct gaaagtactt ttagaggagc tgtttgactt tgctagtttt tgaaactgaa
     7441 atctattata ctacaaacta ttgaaagcgt tttaatttta aggtataata atctcataga
     7501 aataaagaaa aggaggaaag aggatgccac agattagcaa agaagccttg attgagcaaa
     7561 tcaaagatgg aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt tatacagaag
     7621 cgggaggggt gattcccttg ctggtcaaag cggctgagca aggtggagca gtcggtatcc
     7681 gagcaaacag tgttcgcgat atcaaggaaa ttaaggaagt cactaaactt ccaatcattg
     7741 ggattatcaa acgtgattat ccacctcagg aacccttcat cacggctact atgaaagaag
     7801 ttgatgaatt ggcagaactg gacatcgagg tgattgctct ggattgtacc aagcgtgaac
     7861 gctacgatgg tttggaaatt caagagttca ttcgtcaggt taaggagaaa tatcctaatc
     7921 agcttttgat ggctgatact agtatcttcg aagaagggct agcagctgta gaagcaggaa
     7981 ttgactttgt cggaacaacc ttatcaggct acacatccta cagtccaaaa gtagacggtc
     8041 cagattttga attgattaag aaactctgtg atgccggtgt agatgtcatt gcagaaggaa
     8101 aaattcatac accagaacaa gccaaacaaa tccttgaata tggagtgcga ggcatcgttg
     8161 ttggtggcgc cattactaga ccaaaagaga ttacagaacg cttcgttgct agtcttaaat
     8221 aagatgtgag ggggagtttt atgtttaaag ttttacaaaa agttggaaaa gcttttatgt
     8281 tacctatagt tatacttcct gcagcaggtc tacttttggg gattggtggt gcactttcaa
     8341 acccaaccac gatagcaact tatccaatac tagacaatag tatttttcaa tcaatattcc
     8401 aagtaatgag ctctgcagga gaggttgtat tcagtaattt gtcactactt ctctgtgtgg
     8461 gattatgtat tggcttagcg aaacgagata aaggaaccgc tgcgttagca ggagtaactg
     8521 gttacttagt tatgactgca acgatcaaag ctttagtaaa actttttatg gcagaaggat
     8581 ctgcaattga tactggagtt attggagcat tagttgtcgg aatagttgcc gtatatttgc
     8641 acaaccgata taacaatatt caattacctt ccgctttagg attctttgga ggttcacgct
     8701 tcgttcctat tgttacatcg ttctcttcta tcttgattgg ctttgtcttc tttgttattt
     8761 ggccaccttt ccaacaactt cttgtttcta caggtggata tatttctcag gcgggtccaa
     8821 ttggaacttt tctatatgga tttttaatga gactttctgg agcagtaggc ttacatcata
     8881 taatttaccc tatgttttgg tatactgaac ttggtggtgt tgaaactgtt gcaggacaaa
     8941 cagtggttgg agctcaaaaa atattttttg ctcaattagc cgatttggcc cattctggat
     9001 tatttacaga aggaacaagg ttttttgcag gtcgtttctc aacaatgatg ttcggtttac
     9061 cggctgcctg tttagcgatg taccatagtg ttcctaaaaa tcgtcgtaaa aaatacgcgg
     9121 gtttgttttt tggagttgct ttaacatctt ttattaccgg tattacagaa ccaattgaat
     9181 ttatgtttct attcgtcagt ccggttctat atgttgttca cgcattcctt gatggtgtta
     9241 gcttctttat tgcagacgtc ttaaatattt caataggaaa cacattttca ggaggtgtaa
     9301 tcgatttcac tttatttgga attttgcagg ggaacgctaa gacgaattgg gttcttcaga
     9361 ttccatttgg acttatttgg agtgttttgt attatattat ttttagatgg ttcattactc
     9421 aattcaacgt tctaacgcca ggacgaggag aagaagtaga ttctaaagaa atttctgaat
     9481 ccgcagattc aacttcaaat actgcagatt atttaaaaca ggatagccta caaattatca
     9541 gagccttggg tggatcaaat aatatagaag atgtagatgc ttgtgtgaca cgtttacgtg
     9601 tagctgtaaa agaagttaat caagttgata aagcactttt aaaacaaatt ggtgcagttg
     9661 atgtcttaga agtgaagggt ggtattcaag caatctatgg ggcaaaagca atcttatata
     9721 aaaatagtat taatgaaatt ttaggtgtag atgattaagt acttactgac ttaataaaaa
     9781 acagaggaga gtgatggatg agtaggatga aatgaaatcg catacaagaa ataaagaact
     9841 cattatccaa gttggatacg cttattacat aggagaatac aaatgaaatt tagaaaatta
     9901 gcttgtacag tacttgcggg tgctgcggtt cttggtcttg ctgcttgtgg caattctggc
     9961 ggaagtaaag atgctgccaa atcaggtggt gacggtgcca aaacagaaat cacttggtgg
    10021 gcattcccag tatttaccca agaaaaaact ggtgacggtg ttggaactta tgaaaaatca
    10081 atcatcgaag cgtttgaaaa agcaaaccca gatataaaag tgaaattgga aaccatcgac
    10141 ttcaagtcag gtcctgaaaa aatcacaaca gccatcgaag caggaacagc tccagacgta
    10201 ctctttgatg caccaggacg tatcatccaa tacggtaaaa acggtaaatt ggctgagttg
    10261 aatgacctct tcacagatga atttgttaaa gatgtcaaca atgaaaacat cgtacaagca
    10321 agtaaagctg gagacaaggc ttatatgtat ccgattagtt ctgccccatt ctacatggca
    10381 atgaacaaga aaatgttaga agatgctgga gtagcaaacc ttgtaaaaga aggttggaca
    10441 actgatgatt ttgaaaaagt attgaaagca cttaaagaca agggttacac accaggttca
    10501 ttgttcagtt ctggtcaagg gggagaccaa ggaacacgtg cctttatctc taacctttat
    10561 agcggttctg taacagatga aaaagttagc aaatatacaa ctgatgatcc taaattcgtc
    10621 aaaggtcttg aaaaagcaac tagctggatt aaagacaatt tgatcaataa tggttcacaa
    10681 tttgacggtg gggcagatat ccaaaacttt gccaacggtc aaacatctta cacaatcctt
    10741 tgggcaccag ctcaaaatgg tatccaagct aaacttttag aagcaagtaa ggtagaagtg
    10801 gtagaagtac cattcccatc agacgaaggt aagccagctc ttgaatacct tgtaaacgga
    10861 tttgcagtat tcaacaataa agacgacaag aaagtcgctg catctaagaa attcatccag
    10921 tttatcgcag atgacaagga gtggggacct aaagacgtag ttcgtacagg tgctttccca
    10981 gtccgtactt catttggaaa actttatgaa gacaaacgca tggaaacaat cagcggctgg
    11041 actcaatact actcaccata ctacaacact attgatggat ttgctgaaat gagaacactt
    11101 tggttcccaa tgttgcaatc tgtatcaaat ggtgacgaaa aaccagcaga tgctttgaaa
    11161 gccttcactg aaaaagcaaa cgaaacaatc aaaaaagcta tgaaacaata gtccttagtt
    11221 attctataaa aagtagtttt ttaaagaacc taagagtgta tacccccttt tccctctaca
    11281 cagatagtgt aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa
    11341 atgaagttct tacataagcg aatcataaaa aatttcattt tgattttaaa acagttcaag
    11401 aaagtcaaaa aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc
    11461 gtatgcggga aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca
    11521 tctttgtgtt ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga
    11581 ctaaatttga gtttgtaggc ttggataact atatccgtat gtttaaagat cctgttttta
    11641 caaaatctct gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat
    11701 tctcactctt tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc
    11761 gtttcgtctt cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat
    11821 ggatttatga cccactatca gggattctaa actttgtcct taagtccagc cacatcatca
    11881 gccaaaacat ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc
    11941 tcttgaccac ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg
    12001 acaattcact ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga
    12061 agattaaatg gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta
    12121 actcattcca gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa
    12181 caagtacctt gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg
    12241 ccaacacaat tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta
    12301 aagtacttgg aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa
    12361 aaaaaccatt aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc
    12421 tgttcatctt tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa
    12481 ttgttattcc tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca
    12541 tggtgcagaa ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca
    12601 tgttcttagt ctgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct
    12661 atggtcaacg catcctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg
    12721 tccttgtacc attggtacgt attgtcaact tcatgggaat ccacgatact ctctgggcag
    12781 ttatcttgcc tttgattgga tggccattcg gtgtcttcct catgaaacag ttcagtgaaa
    12841 atattcctac agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct
    12901 tctggagtgt agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct
    12961 tcatcaatac ttggaatgac tacttcatgc aattggtaat gttgacttca cgtaacaatt
    13021 tgaccatctc acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga
    13081 ttatggcagg agctgccctt gctgctgttc caatcgtcac agtcttccta gtcttccaaa
    13141 aatccttcac acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatct
    13201 cttcaaacta cgtcagcgtc accttgccat acttaagtat tgcctgcggt tagcttccta
    13261 gtttgttctt caattttcat tgagtatagg aaaatcaatc tatcaagata cagaagtata
    13321 ttttatagat ttagagaata tagaggttat aagtgtctac aaaatggagg gtatgcagtt
    13381 actttatgaa gttttgtcag acacttatca acttaagaat ggttttagtt aactatcaga
    13441 aacgaaggaa agagtatgat ttttgacgat ttgaaaaaca tcacctttta caaagggatt
    13501 catcccaatt tagacaaggc tatcgactat ctctaccaac atcgtaaaga ttcattcgaa
    13561 ttaggaaagt atgagattga tggagataaa gtctttctag ttgttcagga aaatgtcctc
    13621 aatcaagttg agaataatca atttgaacac cataagaact atgcagattt gcatttgctg
    13681 gtagaaggac atgaatattc gagctacggt tcacgtatca aagacgaggc agtagcattc
    13741 gacgaagcga ctgacattgg ctttgttcat tgtcatgaac actacccact cttattgggt
    13801 tatcacaatt ttgcgatttt cttcccaggt gagccacatc agccaaatgg ttatgcaggc
    13861 atggaagaaa aggttcgaaa a
//
LOCUS       DAVMEF010000043        13869 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMEF010000043 DAVMEF010000000
VERSION     DAVMEF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.043"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1124)
                     /locus_tag="VYQ30_000901"
     CDS             complement(<1..1124)
                     /locus_tag="VYQ30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966041.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYL"
     gene            1182..2348
                     /locus_tag="VYQ30_000902"
     CDS             1182..2348
                     /locus_tag="VYQ30_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1966042.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLITSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2487..2813
                     /locus_tag="VYQ30_000903"
     CDS             2487..2813
                     /locus_tag="VYQ30_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1966043.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2800..3393
                     /locus_tag="VYQ30_000904"
     CDS             2800..3393
                     /locus_tag="VYQ30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1966044.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            3386..4297
                     /locus_tag="VYQ30_000905"
     CDS             3386..4297
                     /locus_tag="VYQ30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1966045.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4360..5424
                     /locus_tag="VYQ30_000906"
     CDS             4360..5424
                     /locus_tag="VYQ30_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966046.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEVGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5496..6356)
                     /locus_tag="VYQ30_000907"
     CDS             complement(5496..6356)
                     /locus_tag="VYQ30_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1966047.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFAIVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDVNQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(6631..7617)
                     /locus_tag="VYQ30_000908"
     CDS             complement(6631..7617)
                     /locus_tag="VYQ30_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1966048.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7889..9364)
                     /locus_tag="VYQ30_000909"
     CDS             complement(7889..9364)
                     /locus_tag="VYQ30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1966049.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRIRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9468..10391)
                     /locus_tag="VYQ30_000910"
     CDS             complement(9468..10391)
                     /locus_tag="VYQ30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1966050.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFIF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10405..11334)
                     /locus_tag="VYQ30_000911"
     CDS             complement(10405..11334)
                     /locus_tag="VYQ30_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1966051.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11653..11874)
                     /locus_tag="VYQ30_000912"
     CDS             complement(11653..11874)
                     /locus_tag="VYQ30_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966052.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(11828..11995)
                     /locus_tag="VYQ30_000913"
     CDS             complement(11828..11995)
                     /locus_tag="VYQ30_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966053.1"
                     /translation="MGRDILQTVKDAIASLESIKESQSATKDGGQTAGKETAGEDESA
                     NQTQEISQGIK"
     gene            12155..13501
                     /locus_tag="VYQ30_000914"
     CDS             12155..13501
                     /locus_tag="VYQ30_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1966054.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
     gene            complement(13566..13748)
                     /locus_tag="VYQ30_000915"
     CDS             complement(13566..13748)
                     /locus_tag="VYQ30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966055.1"
                     /translation="MAEAKKALADDSLTEQDLRPNHSYLSQLDVTYKTPDLMSHFLPP
                     HEVSFTFCCSSIVFPR"
BASE COUNT         4099 a         2978 c         2563 g         4229 t
ORIGIN      
        1 tccaaataaa ttattgttcc cttgtactcc ttcttccatt taaatcctct aattaaacct
       61 acaatcgtcc caaagccata agcaaagtga atggaaaata aaagaaaggg catcacaatt
      121 agaaatccat ttttatgttt taataaagtc agcaaagtga gtagtgacaa aagtagaaaa
      181 taggcaccta atagtaaagt tatgaatacg aatgtgatcg gtaacaatgc tagactaaac
      241 acaagactca aaacaaataa acaaggaaca tagtgaaata atgataaaca cttaaactga
      301 acatgacttg tcaagccaat ccacaaacca tttgaatact tttgatgcag cattttcttg
      361 aatgttggtc gaatatactg ataagataga atacttgggc tatagcggat tttataacca
      421 tgttctcgaa ttctataatg aatatcatta tcttcagttc ggccaagttg ctcatttact
      481 aaaccaacct tctggaaaac ctctcgttta tacattccat gaaaaataga agaaacatat
      541 ctatcctcag aactatttcg ataattggca atgctactgc caaacatatt ttcctcaaca
      601 agatgcaagg tctctgccca ttttcctttt ccttcgacaa tcgtcggtct aggcccccca
      661 cagacaaatt caccttgttg aataatagcc acattgttca ttacaaaagt ctcagtaact
      721 tttgaatgag catcaatttt taaaataagg tcccctacag aatgtttaac tcccaggtta
      781 aaaccactag cttgattttt cttaggattg ttatacaatc taattgagtt aaactctgta
      841 tcttccttta taaattgctg aatgatagct gtggtcccat ctgtggacat agcatttata
      901 aatagaattt caatattctc tttaggataa gtttgatttt ttaagtcttc aattagacca
      961 ggcagatatt tttcttcatt gtatgcactg attacaatgg atacgagaag actttcaaca
     1021 ttttgagtca ctactactct cctgaatttt tccttccacc attctatcat aattttcaag
     1081 actttcccat ttttatcttg aaagccagaa accttacttg acattatagt gaaaaagcct
     1141 taaaataagc tgttattaaa atcagctaga agaggtatta tatgaaaaag caatcactct
     1201 tttttgttcc aggtattatc ctgattggtg tttccttgcg aactcctttt actgttttac
     1261 ccattatttt gggaaatatt tcgcaaggtc tggaggtaga agttagttcg cttggtgtct
     1321 tgaccagcct gcctctcctt atgtttaccc tcttttcacc attttctacc caactggctc
     1381 agaaaatcgg cttggagcat ctcttcacct acagcctctt cttcttgacc atcggctcac
     1441 ttattcgact aatcaatctg cccctactct atctaggaac cttgatggtt ggggcaagtg
     1501 tcgcagtcat caatgtcctg cttcctagtc ttatccaagc caatcaacca aagaaaattg
     1561 gttttctgac caccttatat gtaacgtcta tggggattgc aacggctctg gcttcctatc
     1621 tagctgtgcc cattacacaa gccagttctt ggaaaggact tatccttctc ctcacgttac
     1681 tctgtctagc aacttttttg gtctggctcc caaatcaccg ctataatcat agactagctc
     1741 cacaaaccaa acaaaaaagt caaataaagg tcatgcgtaa taaacaggtt tgggcaatta
     1801 ttatcttttc aggttttcaa tccttgatct tttacaccgt catgacctgg ttacctacca
     1861 tgtctatcca tgcaggtcta tccagtcacg aagctggctt gctgacttct atcttatctc
     1921 tgattagcat tcctttttca atgaccatcc caagcctgat aaccagttta tctactcgca
     1981 accgtcagct catgctcact ctggtttcac tagctggtgt ggtcggcatt tccatgctct
     2041 ttttcccaat caataatttc atttactggc ttgccatcca tctcctcatc ggaaccgcaa
     2101 ccagtgccct cttcccttat ctcatggtca acttttcact caagacaagc gcccctgaaa
     2161 agacagccca attgtccggc ctatctcaaa caggaggcta tatcctagca gcctttgggc
     2221 caaccctctt tggttacagt tttgacctgt tccactcttg ggtaccatct gtagctgccc
     2281 tcttgctcat cgatatcctg atgactgtgg ccctctttac agtggacaga gcggataaga
     2341 tcctttaaac ttctagtttt agctagaagt tttttatata taaaaatttt acacatttct
     2401 cttgacaggg ctttctttta gatgtacaat gtatgtgtag aaaaattata tataaaaatc
     2461 ttacacatta gaaaaggagg tttcccatgt actttccaac atcctctgcc ttgattgaat
     2521 ttctcatctt ggctgtactg gagcagggtg attcttatgg ttatgagatt agccaaacca
     2581 ttaagctcat cgctaatatc aaagaatcca cactctatcc cattctcaaa aaattggaag
     2641 gcaatagctt tctgacaacc tattctagag agttccaagg tcgcatgcgc aaatactact
     2701 ccttgacaaa cggtggtata gagcagctct tgaccctaaa agatgaatgg gcactctata
     2761 cagacaccat caatggcatc atagaaggga gtatccgcca tgacaagaac tgaatacctg
     2821 actcagctag aactctatct caagaaacta cctgaagctg accgtatcga agccatggac
     2881 tatttcagag agctctttga cgatgctgga gtcgaaggag aagaagaact catcgctagt
     2941 ttgggaactc ccaaagaagc ggcccacgaa gttctatcca atcttctcga taaaaaaatc
     3001 aatgaagcac ccgctcaaaa aaataaccga caaattttac atatcgcctt gttagccctc
     3061 cttgcagcac ctatcggcat tcctctggga atcgccatcc tcgtgaccct gttcgcaatc
     3121 cttgtagccg ctttgactgt cattctggct ttctttgcag tttccatact gggtatcatc
     3181 ggcggattcc tatttttagt tgaaagtttc actgtcctcg cccaagccaa atcagccttt
     3241 atcttgattt ttggttctgg tttactggct atcggtgctt cttcgctagt tttactaggc
     3301 atttcctatg tagctcgctt cttcggtcta ctcattgttc gcctggtaca atttgttctt
     3361 aaaaaaagaa agagaggtaa ccagcatgcg taaatggaca aaaggatttc tcatctttgg
     3421 tgtggtgact accgttatcg gctttatcct gctttttgta ggcatccaat ctgacgggat
     3481 taagagccta ctttccatgt ccaaagaacc tgtctatgat agccgtacgg aaaagctaac
     3541 ctttggcaag gaagtcgaaa acctagaaat tactctccac caacacacgc tcaccatcac
     3601 agactctttc gatgatcaaa tccacatttc ttaccatcca tctctttctg ctcaccatga
     3661 tcttatcacc aatcagaacg atagaactct gagtctcact gataagaaac tgtctgaaac
     3721 tccgtttctc tcttctggaa ttggtgggat tcttcatatc gcaagtagct actctagtcg
     3781 ttttgaagaa gttattctcc gactaccaaa agggagaact ctaaaaggga tcaacatctc
     3841 agccaatcgc ggacaaacca ccatcataaa tgctagcctt gaaaatgcga ccctcaatac
     3901 aaacagctat atcctccgaa ttgaaggaag tcgtatcaaa aacagtaaac tcacaacgcc
     3961 caatatcgtt aatatctttg atacagttct tacagatagt cagctagagt caacagagaa
     4021 tcacttccac gctgaaaata tccaagtcca tggtaaggtt gaactgactg ccaaagatta
     4081 tctcagaatc atcctagacc agaaagaaag ccaacgaatt aactgggaca tctcaagcaa
     4141 ctatggttct atcttccaat tcacaagaga aaagcctgaa tcaagaggta cggaattaag
     4201 caacccttac aaaactgaaa aaaccgatgt caaggatcaa ctcattgcga gatctgatga
     4261 taatattgat ctaatatcca caccaagcag acgttgacaa aaacgcttac atctgctact
     4321 atggaagcat atttatctaa ctaaaaagga ggttctacca tgaaacaaga atggtttgaa
     4381 agtaatgatt ttgtaaaaac aacaagcaag aacaagcctg aagagcaagc tcaagaggtt
     4441 gcagacaagg ctgaagaaac gatagccgat ttcgatacac caattgaaaa aaatactcag
     4501 ttagaggagg aagtctctca agctgaagtc gaattggaaa gccagcaaga agagaaaatt
     4561 gaagctcctg aagtcggtga agcgagaaca gaaatagaag aaaagaaggc atctaattct
     4621 actgaagaag agccagacct ttctaaagaa acagaaaaag tcactatagc tgaagagagc
     4681 caagaagctc ttcctcagca aaaagcaacc acgaaagagc cacttcttat cagtaaatct
     4741 ttagaaagtc cttatatccc cgaccaagct ccaaaatcta gggataaatg gaaagagcaa
     4801 gtgcttgatt tttggtcttg gctagtggaa gcgatcaaat ctcctacaag taagttggaa
     4861 acaagtatca cacacagtta cacagccttt ctcttgctca ttctgttttc tgcatcttcc
     4921 tttttcttta gtatctatca catcaaacat gcttactatg gacatatagc aagcattaac
     4981 agtcgcttcc ctgagcagct agctccttta actctttttt ctatcatctc tatcctagta
     5041 gcgacaacac tcttcttctt ttcattcctc ttgggtagtt tcgttgtgag acgatttatc
     5101 caccaggaaa aggactggac gctagacaag gttctccaac aatatagtca actcttggca
     5161 attccaatct tcctcaccgc tattgctagt ttctttgctt tctttgatag cctacgattt
     5221 acagctctct tgtgtgtgat tagcattgga atcattctgc ttgccagtct ccatatcatt
     5281 acaagaccaa gtcaagcaag tgaaaccgac tccttctatc aattattctt gtctgtcctt
     5341 gtgaacggag ttattatcct cctcttcttt gtagctgaag tggcactgat tggagattat
     5401 cttcgtatct tggcctttct ttaaaattca atcctgtcat ccttggcagg attttttcta
     5461 tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact tttcttgtgc taatgtttta
     5521 aaatcttgtc ctaggatggg ttgtatttct aattgattga cgtctagctg gtggtaaaaa
     5581 gatgggggta acacctctaa gaattttgca tagtccaagc gagctatgga ttcatagtct
     5641 tctggtttag agccgtctcc tgtgatttga accaagtcaa tctcccactg gacttcctta
     5701 tccaccaatt gctcaatata ggctgcagga acaaacggtt ctctcggtaa aactgtcttg
     5761 actccttgag ctagatggta aacgccatgc acttttcctt ctccatcctg atagaaggaa
     5821 actatcgcaa agtaggcatc tgtctcttga accgcacgca cacgcgcttc aaactgagcc
     5881 aagccatctg cctctaaaaa gcttttcaag ttctcacgac taaagaaaat aggattaaaa
     5941 attccttggc aaatcgtaaa acgagctaca gggtctgcta gataggcttg aatacttgct
     6001 tggaaataag cttcaaaatc aaaaccatct agcccttcaa tatcagtccc tgtataagca
     6061 agcaaagcat ctaaaaatga cttgataatc tgccagtctg tctttgttag taggtcagga
     6121 agatcgacac ggtaagcctt ttgaccatca gcataactga ccttaaaaag aaattgtgat
     6181 tgccccacta tcgcacactc aatatactca agacgattaa gaggctgacg gagatagact
     6241 acatcatagc tatgtgactc caaaccgtct agcaaggcca agatcgactt ggcattcaaa
     6301 atctcttgtt ctcctaaaat gctctgttta tttggaataa aaaatgtttt cgccataact
     6361 ggactccttt gaaatcgttt acatacagta taccttattt ttctgaaaga tacaaaaaca
     6421 aactttatac tcttcgaaaa tctcttcaaa ccgcgtcagc ttcgccttgc cgtagatata
     6481 tgtaactgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag cagcctgcag
     6541 ctagcttcct agtttgctct ttgattttca ttgagtatta gatttttgag tacaaagcat
     6601 agaaaaacag cccctgagga ctgtttgatc ttatacagta gctgcttgat ccaagctttc
     6661 accgatagcg gctaggcgct cgatcacttc agcttgtgtc aattcatttt ttgaaacata
     6721 gcggttacgt gggtgaacac ggcactcgtg tgagcatcca cgaaggtact tgtcttcatt
     6781 ttcttctgat gtcaagatac gacggttaca gaagggattt ccacagttga cataacgttc
     6841 acatggtgtt ccatcaaacc agtctttccc tacgatggtt gggttgacat ggttgacatc
     6901 aacggcgata cgctcgtcaa atacgtacat ttttccatcc caaagctcac cttgaacttc
     6961 tgggtcttta ccgtaagttg cgattcctcc gtgcaattgg ccgacatctt tgtagccttc
     7021 acggaccatc cagcctgaga atttctcaca gcgaacgcca cctgtacagt aaaccacgac
     7081 acgcttgtcc atgaattttt ccttgttatc acggacccat tgtggtaact cacggaagtt
     7141 gcgaatatct gggcgaatag ctccacggaa atgtcctagg tcgtactcat aatcgttacg
     7201 tgtgtcaagg acaacggtat ctttatcaag aagcgcttct ttgaactctt ttggagacaa
     7261 gtaagcacct gttgtttcaa gtgggttgat gtcattgtca aagtcgttgt cttccaaacc
     7321 aaggtggaca atttctttct tgtagcgaac aaacatcttc ttgaaggctt gttcattttc
     7381 ttcgtcaatc ttgaaccaaa gttcttccat tcctggaagg ctgtgaacgt agtccatgta
     7441 tttttgagtt gtttcatagt cacctgaaac tgttccgtta attccctcgt cagcgactag
     7501 gatacggcct ttaaggccga ttgatttaca gaaagccaag tggtctgcag caaattgctc
     7561 tgcattttca attggagtat aaaggtagta aagtaagaca cgaatatctt ttgccataag
     7621 atttgttctc ttttctattc ttaaattttc taaatttttt atttatccat actagtatac
     7681 ctgcttgaga aaacgaatgc aatttatttg actggaaatt gtagagggtt ctcgaaattt
     7741 cctgaatggt taaaataagg acaagagaaa acatggatat ctatatcctt gtgccaaaaa
     7801 aaccactgcc ctccccagac caacctgagg aaagcagtga ttcttatttt aggagttagg
     7861 aatgaataca cgaaatcaat ttagctgatt attttttgtt tttcaagaat tcatcgtatt
     7921 gtttttgcat ttcgttcaat actttttcgt aggcaccttc agatttcaat ttttccatca
     7981 attctggaat cgctttatct gggtctacag taccagtgtt gatagctgta tcaaattgtt
     8041 gcattgtgtt agcaatagct gagatttcag atttcacatt gtcagtattg aagataaatc
     8101 caagcgctgg agattcttta gcttctgcca attctttctt agaattttcg atttgttggt
     8161 ctgtaacgtt ttcgttgatg taaaggatcc agttgttacc agtgttccat ccacccatgt
     8221 gagtgtttcc tttgtagcca tcaagaacgc gaatacggtt ttctttacct tcaatttttt
     8281 cccagttctt gccttctgga ccgtaaacaa gaccgttcaa gagttctggg ttcgtattca
     8341 agaggttcaa gatttccatt gatttttctt tgttcttaga gttgtttgag atgacaaagt
     8401 tagcaacttg tgttgtttgg tttttcttga tgaagttagt aattggtttg atttggatat
     8461 ctttgttggc aacacgtgaa agcaagctgt taccgtagtc agctggtcct actgtttctt
     8521 cacgaacgaa ccaagtatct tgttgaaggt caaatgaagt atcgcttgtt gcgacatctt
     8581 ttggaatgta gccagcttca tagaatttgt gaagagtctt caagtgttct ttgaaacgag
     8641 gcacttcgta acggtttaca actttagtag tatcgccttc aaggtcaata acgaatggaa
     8701 gcccgtttgc tactgggtag tcaaaattat cagatgggat gaaaacttta ccaatagcaa
     8761 atggtactac gtctggagct ttttctttga tttgtttcaa gactggctca agagtttcgt
     8821 aagaagtaac acctgaaata tcgataccat atttggcaag gagagttccg ttgaaggcaa
     8881 agttttgaga tgatgcaacg ttggctgcaa ctggtacagc ataaatctta ccatttacag
     8941 tattaccctt gatataagct gggtcaagtg ccttgtaaag gtctttacct tcttttttgt
     9001 acaattctgt caagtcagca taagcacctt tttgagcatt tacaatatag ttatctgcaa
     9061 aggcgatatc atagttttca ccagatgatg tgataactga cattttctta ccatagtcac
     9121 cccagccaag gtattggata tccaatttag caccaacttt ttcttcgatg attttgttgg
     9181 catttgctaa caattcatcc aagttatctg gtttgtcacc gatttggtac attttgataa
     9241 caggtttgtc acctgaatca gcagcttttt tactgttacc tgtcaagttg ccacaagcag
     9301 caagacctgc agccaaagcg actacactag cagatgcaaa agcatatttt ttccagtttt
     9361 tcatgataaa aactcctttt tttattttta aacttataaa caatgtaatg atcttaactt
     9421 cacgcaaggg aagtttggaa atgagaaatg ttctttttca ataagcacta ttctttcaca
     9481 ccaccgatag tcaaaccttt tacaaagtag cgttggaaga atggatacaa aatcgcgatt
     9541 ggaagggttg ctaccacaac catggccata cgacctgttt ctttcggtag agcaactccc
     9601 agttgaccag tcaagccgac cgctttggca atgtagtcca tattttgttg gatttgcatg
     9661 agcaaatatt gcaatggata caagttatca ctcttgatgt aaagaagggc gttgaaccag
     9721 tcattccaga aaccaagagc tgtcaaaagc gtgatggttg cgatacctgg tagtgacaat
     9781 ggcaaacaga tttggaagaa aatccgagcc tcactggcac catcgatacg agccgattcg
     9841 agaatggctt ctggaatggt cttcttgaaa aaagaacgca tcaagatgat attgaatggt
     9901 gagagaagca ttggaacaat caaggcccaa acagtgtcac caagctgaag tacacgggtc
     9961 accatgatat aacctggtac caaaccagcg ttgaacaaca tactgagaag gacgaagatg
    10021 gtaaaaaatc tgcgatactt aaaggttgtc cgtgaaatag cgtaggcata ggttgttgtg
    10081 ataaagacat ttgtcaatgt cccaactacg gttacaaaga cagagatgaa gagggcttgt
    10141 aggattttat ccttaaactg tgccaaaaac tcaaaaccgt ctaagccaaa ttgggatggg
    10201 aagaagctat agccgtattg gaggaggctt ttctcgtctg tcactgaaat aatgataacg
    10261 aatataaaag gtaggataca agagagggca atcaaacccg aaatgatact gaagaagata
    10321 tctgctttct tactgaagga gtgaatgccg acattatcaa ttttttcttt tttaattttc
    10381 ttttttgcca tattctcctc ctttctagaa caaagctgag tttggatcga ctcgtcttgc
    10441 aagcaagttt gataggataa ccagaatcaa accaacaacg gattggtaaa gaccggctgc
    10501 tgcagccata ccgatatctg ctgtctgagt caaaccatta aagacatata cgtccaaaac
    10561 gttggttaca ttgtaaagct gaccagcatt gtgtgggatt tgatagaaga gaccgaagtc
    10621 tgcgcggaag atatttccga ctgcaaggat ggtcaataca gttacaagcg gagtcaactg
    10681 aggaatggtt acgttgcgaa tacgttgcca cttgctagct ccgtccactg tcgctgcttc
    10741 gtagtaggtt ggatcaattc ccatgatcgt cgcatagtac atgacactgc tatatccaaa
    10801 gcctttccaa atacctagga aaagtaggag atagggccag atgcccaggt cagcgtaaaa
    10861 attgacttct ttgagaccaa gactttccaa tagatgattg aacacccctt tatcaatatt
    10921 taggaaggca tctgtaaaga aactgatgat aacccaagac aagaagtaag ggaacaacat
    10981 agaagtttgg aaaatcttca ccattctctt agaacggagc tcgctgagga taatggcaat
    11041 ccctacagat acaactaaac ctagaaagat aaagccaaga ttgtagagga cagtatttcg
    11101 tgtgataata aaggcgtctc ttgaactaaa taagaatcta aaattatcga gtccgaccca
    11161 tttactattt atgatactat ctatgaaacc attactggtc atgtggtaat ctttgaaggc
    11221 aaccacattc ccaaatactg gaatgtaaaa gaatagaatc aaccagagtg cccctggcaa
    11281 aaccatcaag agaaagatcc agttgtctct caatgttttt gaaaactttt tcataatttc
    11341 ctcccttttt attttgatat ccatctaaaa attctttttt agacttttga taacgattac
    11401 attattagta tactcctatt tgcaggttag gttaaactcc taattataga aaaaactcca
    11461 caaattatgt agcagattta aaactttatc accactatca aacaaatgtc ctaaatcaat
    11521 tgtttatttt atctctatta gcccagtgat ggcgtcactc tgttataagc atccaacaac
    11581 ggggtatact gaaaaatctc cagactaggg aactcagcga tagttcctaa tctggagatt
    11641 tttaatatgt tattaggcgt ttgctttcaa cttagcaata acctctttaa gattatcaat
    11701 caactctgct gcagtatgct cagagccttt ttcatctgcc aagaacaaaa ctgctttttg
    11761 aagttcttct tgagagtttt caaggacatc cttatctact gtttcaaggt ttgagtcttt
    11821 aagaagttta cttaattcct tggctaattt cttgagtttg atttgcagac tcatcttctc
    11881 ctgctgtttc tttgcccgct gtttgtcctc catccttagt tgctgactgg ctttccttaa
    11941 tggactctag ggaagcaatg gcatctttga ctgtttgcaa gatatcacgc cccaattatt
    12001 caccccaaat ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct
    12061 tttttcaccc aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag
    12121 tgtagaaaaa aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt
    12181 ccaaaacaag tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg
    12241 tcttatcttt tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta
    12301 tttagtaacg aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca
    12361 gttcctcttt caactgttaa caggctatgg aacggactat gcttgtaaag aattgtcagc
    12421 tgatgcctac tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc
    12481 ccgttttctt tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga
    12541 attggtcgaa ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc
    12601 tacccatttc acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc
    12661 tcatggctat catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca
    12721 gcttcgtcct ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt
    12781 agaacggttt aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata
    12841 cgatttaatt gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct
    12901 gagccgtctt ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc
    12961 ccactccgcc tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt
    13021 ctgccagttc tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac
    13081 aaatatgacg agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc
    13141 cgagaatttc atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac
    13201 cttaatcaaa aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt
    13261 tctcaaacat ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct
    13321 ttttcttcac gtggtaggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc
    13381 tagtctctat gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa
    13441 cctgaatctt cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta
    13501 aagaacagtc gagatgaaaa aatcgtgtga cgaaccaagg gaggagtctg cccttttgag
    13561 gaaatctagc gaggaaaaac gatactggaa cagcagaaag taaaactgac ctcatgagga
    13621 ggaagaaagt ggctcatgag gtcaggggtt ttgtaagtta catctagttg agagaggtat
    13681 gaatgatttg ggcgtaaatc ttgctctgtc aaactatcat ctgcaagagc tttcttagcc
    13741 tctgccaaaa ccagacgtgc tgaatctgtg gtaggattcg atacacctgt caatgatctc
    13801 aaaagatttt ctaaggtttg agtctactta ctaatactag actaaaatca aaaagcatta
    13861 cataacagt
//
LOCUS       DAVMEF010000044        13862 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMEF010000044 DAVMEF010000000
VERSION     DAVMEF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.044"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            75..872
                     /locus_tag="VYQ30_000916"
     CDS             75..872
                     /locus_tag="VYQ30_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1966056.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGSLIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1020..1967
                     /locus_tag="VYQ30_000917"
     CDS             1020..1967
                     /locus_tag="VYQ30_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1966057.1"
                     /translation="MTCDFKFETLQLHAGDHVVAASTIYGGTFNLLKEPLPRYGITTT
                     FVDVDNLEEVEAAIKDNTKLVLIETLGNSLINIPDLEKLAEIAHKHQIPLVSDNTFAI
                     PYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKFPQFVDEGPSCH
                     NLSYTCDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETSSLRVERHVQNAETI
                     VDFLVNHPKVEKVNYPKLADSPYHALDEKYLPKDVGSIFTFHVKGGEEEARKVIDNLE
                     IFSDLANAADAKSLVVHPATTTHGQLSEKDLEAAGVTPN"
     gene            2138..3412
                     /locus_tag="VYQ30_000918"
     CDS             2138..3412
                     /locus_tag="VYQ30_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1966058.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEVDNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3422..4300
                     /gene="truB"
                     /locus_tag="VYQ30_000919"
     CDS             3422..4300
                     /gene="truB"
                     /locus_tag="VYQ30_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1966059.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASFTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4541..4843
                     /locus_tag="VYQ30_000920"
     CDS             4541..4843
                     /locus_tag="VYQ30_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1966060.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKHQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFSLWIAKAPSQERGRLAFLQMLAQILQGFR"
     gene            4951..5589
                     /gene="udk"
                     /locus_tag="VYQ30_000921"
     CDS             4951..5589
                     /gene="udk"
                     /locus_tag="VYQ30_000921"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1966061.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5717..7057
                     /locus_tag="VYQ30_000922"
     CDS             5717..7057
                     /locus_tag="VYQ30_000922"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1966062.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7035..7247
                     /locus_tag="VYQ30_000923"
     CDS             7035..7247
                     /locus_tag="VYQ30_000923"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1966063.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7244..8119
                     /locus_tag="VYQ30_000924"
     CDS             7244..8119
                     /locus_tag="VYQ30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1966064.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVAIKSAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8112..8927
                     /locus_tag="VYQ30_000925"
     CDS             8112..8927
                     /locus_tag="VYQ30_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1966065.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8920..9351
                     /locus_tag="VYQ30_000926"
     CDS             8920..9351
                     /locus_tag="VYQ30_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1966066.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9358..11025
                     /gene="recN"
                     /locus_tag="VYQ30_000927"
     CDS             9358..11025
                     /gene="recN"
                     /locus_tag="VYQ30_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1966067.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFLDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11027..11755
                     /locus_tag="VYQ30_000928"
     CDS             11027..11755
                     /locus_tag="VYQ30_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1966068.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11834..13657
                     /gene="lepA"
                     /locus_tag="VYQ30_000929"
     CDS             11834..13657
                     /gene="lepA"
                     /locus_tag="VYQ30_000929"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1966069.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4109 a         2522 c         3302 g         3929 t
ORIGIN      
        1 gaaatacaga aaacgcttcc taaaacctaa agtttgtgat ataattatca agaaaaaaga
       61 tttaggagtt tattatgatt tcttcagaat ttatctcaaa gattgaattt gcttgcaata
      121 agaaagaaag tctttatagt caaagcaaat ttaagtatgc gattcgttcg atattcgcag
      181 gtgcattttt aaccttcagt actgctgcag gtgcagttgg ggctgacttg attaataaaa
      241 ttgcaccagg tagtggacgc ttcctctttc cattcgtttt tgcttggggc ttggcctaca
      301 ttgttttttt gaatgccgag ttggtcactt caaacatgat gttcttgact gctggtagtt
      361 tcttaaaaaa aatctcttgg agaaaaacag ctgagatttt actatactgt accttgttca
      421 accttatcgg atccttgata gcagggtggg gctttgctca ttcggcagcc tatgcgaatc
      481 tgacacacga tagtttcatc tcaggtgttg ttgagatgaa gttaggccgc tcaaatgaat
      541 tggtcttgct tgaggcgatt ttggcaaata ttttcgtaaa tattgcgatt ctgtcattta
      601 ttttggtcaa agatggtggt gccaaacttt ggcttgtgtt gtcagctatt tacatgtttg
      661 tattcttaac aaacgagcac attgcggcga actttgcttc tttcgcgatt gtgaaattca
      721 gtgttgctgc ggattcaatt gccaacttcg gtgttggaaa tatgcttcgc cactggggtg
      781 tgactttcat cggaaacttt atcggaggag gcctcttgat gggtcttcca tatgccttcc
      841 tcaataaaaa cgaagatact tatgtagatt aagaaaatga gcacgattga gtcgtgcttt
      901 tttcattttc aaaataaggt aacagctatt tcttatatca aaatatagaa aactgatatt
      961 tgtaaactat aactcaaggt gctacaatat ccttaataaa ataatatgga ggtcacctta
     1021 tgacttgtga ttttaaattt gaaactctac aactacatgc tggtgaccat gtagtggctg
     1081 cttcgactat ttacggtgga accttcaatc ttttgaaaga accccttcct cgttatggta
     1141 tcacaacaac ctttgtcgat gttgataatt tggaggaagt agaagcagct atcaaagaca
     1201 ataccaagct tgtcttgatt gaaaccttgg gtaactcctt gattaatatt ccagacctgg
     1261 aaaaactggc agagattgct cataaacatc aaatcccact tgtgtcagac aatacttttg
     1321 caatacctta tttgattaac gtcttctctc atggcgttga cattgccatt cactctgcga
     1381 ctaagtttat cggtgggcat ggtacaacta ttggaggaat aattgtcgat agtggtcgtt
     1441 ttgactggac ggcttcaggg aaattccctc aatttgttga cgagggtcca agctgccaca
     1501 atttgagtta tacttgtgat gtgggtgcag cagcctttat tatagctgtt cgagttcaat
     1561 tgcttcgtga tacaggtgca gccttgtcac cattcaatgc tttcctcttg ctacaaagac
     1621 ttgaaacctc ttcacttcgt gtggaacgcc atgtacaaaa tgctgagaca attgttgatt
     1681 ttcttgtcaa ccatcctaag gtagaaaagg taaattatcc aaaacttgca gatagtcctt
     1741 atcatgcctt ggatgagaaa tacttgccaa aagatgtcgg ttcaatcttt accttccatg
     1801 tcaaaggtgg cgaggaagaa gcacgcaagg tcattgataa tttagaaatc ttttctgacc
     1861 ttgcaaacgc ggcagatgct aaatcgcttg ttgtccatcc agcaacaacc actcacggtc
     1921 aattgtcaga aaaagaccta gaagcagcag gtgtcacacc aaactaaatt cgtttgtcaa
     1981 tcggtcttga aaatgtagaa gatttgattg aagacttgcg cttggccttg gaaaaaattt
     2041 aaagtaaaag aagataaaca gtgggcttcg actcactgtt tttgattttc cctcaggcat
     2101 gatataatgg ttaaagaagt ctagaaagag gaacgatatg aacgaaatca aatgtcccaa
     2161 ctgtggggaa gtctttacag taaatgagag tcagtatgcc gaactcttgt cccaagtgag
     2221 aacggcagag tttgataagg aactacacga taggatgaag caggaactgg ccttggctga
     2281 gcaaaaggcc atgaatgagc aacagactaa actggctcag aaggatcaag aaattgcgca
     2341 attacagagt cagatccaaa actttgatac agaaaaagaa ttggccaaga aagaggttga
     2401 acagacaagc catgaggctc tcttggctaa ggacaaggaa gtacagctct tagaaaatca
     2461 gttggctacc ttgcgtttgg agcatgaaaa tcaactgcaa aagacccttt ctgacctaga
     2521 aaaagaacgg gatcaggtta aaaaccaact acttttgcag gaaaaggaaa atgaattatc
     2581 tttggcttct gttaagcaaa actacgaagc ccagctcaag gcagctagtg aacaagtcga
     2641 gttttataag gattttaagg ctcaacaatc tacaaaagcg attggggaaa gcctagaaca
     2701 gtatgcagag agtgagttta acaaggttcg tagtttcgcc tttccaaatg cttactttga
     2761 gaaggataac aaggtctctt cgcgtgggtc taaaggggac tttatcttcc gtgagtgtga
     2821 tgaaaatgga gttgaaatca tttctatcat gtttgagatg aaaaacgaag cagacggaac
     2881 agagaagaag cacaagaatg cagattttta caaggaattg gacaaggacc gtcgggagaa
     2941 gaactgtgag tatgccgttt tggtgaccat gcttgaggtc gataatgact actttaacac
     3001 agggattgtt gacgtcagcc acgagtatga aaaaatgtat gttgttcgtc ctcaattctt
     3061 tatccagttg attggtctct tacgtaatgc ggcgctaaat tccctaaaat acaagcagga
     3121 gttggccttg gttcgcgagc aaaatattga cattacgcat tttgaggaag acttggatgc
     3181 ctttaagcta gcttttgcta agaactataa ttcagcttcg actaactttg gaaaagctat
     3241 tgatgaaatc gacaaggcca tcaaacgcat ggaagaggtt aagaaattcc tgactacatc
     3301 tgaaaaccaa ctccgtctag ctaacaacaa attggaagat gtctctgtta aaaaattgac
     3361 ccggaaaaat ccaacaatga aagcgaagtt cgaagcattg aagggggagt agaaagcaaa
     3421 aatgaacggt attatcaact taaaaaaaga agcaggaatg acctcgcatg atgcggtttt
     3481 taaactgcgt aagattttgg gaaccaagaa aattggtcat ggcggaacct tggatccgga
     3541 tgtggtgggt gttttgccga ttgcggttgg caaggcgaca cgcatggtcg agtttatgca
     3601 ggacgagggt aagatctatg agggggaaat cactctgggc tattccacga cgactgagga
     3661 tgctagtggg gaagtggtcg cagaaacccc tgttttgtct cccttggatg aaaagcttgt
     3721 tgatgaagcg attgctagct ttactgggcc tattacccag attcccccta tgtattcggc
     3781 agttaaggtt aatggtcgca agctctatga gtatgcgcgt gctggtcagg aagtggagcg
     3841 tccagaacgt caggtgacca tttatcaatt tgagcgaaca agtccgattt cttatgatgg
     3901 ccaacttgcc cgattcactt ttcgtgtaaa atgcagtaaa gggacttaca tccgtacttt
     3961 gtcagttgat ttgggtgaaa agcttggtta tgcggctcat atgtcccatt tgactcgtac
     4021 tagtgctgct ggcttacaat tagaagatgc tcttaccttg gaggaaattg ctgaaaaagt
     4081 agaggctggg caattagatt ttctccatcc tttagagatt gggacaggtg accttgtcaa
     4141 agttttccta actccagaag aggctacaga agttcgcttt ggtcgtttta ttgagctaga
     4201 ccaaacggac aaagaactgg ctgcctttga agatgataaa ttgttagcca ttctagaaaa
     4261 acggggcaat ctctataagc caaggaaggt ttttagctag atcgtttagg aataaaaatc
     4321 gggtgataga taacaattgc ttgataaaac cccatactaa tagtagaatg gttttgggaa
     4381 ttataatatt ccaattgttg cgagttgtag gtactcaaat aatctatata gaaatttaga
     4441 ggtgtgaaat gaagcaattt aaaattcttt cagataaata tttagagtcc attacaggtt
     4501 ctggtgggaa cttaggccca ggatttggtg tgataattcc atgatgcgaa atgagtttcg
     4561 agaaagggtg gagcaacttc ttcaacaaaa agaaataaat gaaaatagtg agttgagtca
     4621 cctgtttcgt cttgctatac aaaatttaga cagaaatgaa aaacaccaat cggtcatggc
     4681 caatttgagt caagggttgt cactttacct catgacgcat cattaccagg cacctaagtc
     4741 tgtcattgat tttagtttat ggattgccaa agctcctagc caggaaagag ggaggctggc
     4801 tttcttgcag atgcttgctc aaatcctaca gggctttcgt taaaactgtc gaacatcaga
     4861 gggaagactc tggtgttttt tgctgatgag actataaatt ttgtaaaaaa tgtgatagaa
     4921 tagacgacgg ataaaataac ggaggatagc atgcaaaata gaccaatcat tattggagtg
     4981 acaggtggtt ctggtggtgg taaaaccagt gtttcaagag ccattttatc gcattttcct
     5041 gatgaaaaga tttccatgat tgagcatgat tcatactaca aggatcagtc tcatttgacc
     5101 tttgaagagc gtgtcaaaac caactatgac catccttttg cctttgatac agacttgatg
     5161 atcgagcaga ttaaggaatt gttggcaggg cgtccggtgg acatcccgac ctacgactat
     5221 acagagcata cacggagtag caagacctat cgtcaggagc ctcaagatgt ctttatcgtt
     5281 gagggcattt tggtcttgga ggacaagcgt ctgcgcgatt tgatggatat caagattttt
     5341 gtggatacgg atgacgatgt gcgcattatt cgtcgtatca agcgtgatat ggaggagcgt
     5401 ggccgtagcc ttgatagcgt tattaaccag tacttaggtg tggtcaaacc aatgtaccac
     5461 cagtttatcg agtcaactaa gcgttatgct gatatcgtca ttcctgaagg ggttagcaat
     5521 accgtggcta tcgacctgtt gacgaccaag attgcaaaga ttttggaaga agctcgaaac
     5581 agcaaataat cacatgtgga ggccttgcct cctttttcta tttttccctc ataatggtac
     5641 ataaatggag atttttaggc atatttttgg tataataata cccatgaaag agcaagtaaa
     5701 tgaatagtag gtggagatgg aaaagtattt atcggtaaca actttgacca agtatctgaa
     5761 aatgaaattc gataaagacc catacttgga acgggtctat ttaactggtc aagtttccaa
     5821 ctttcgtaaa cgacctactc accaatattt ctccctaaag gatgaccatg cagttattca
     5881 agcgaccatc tggtctggga tttatcagaa attagggttt gacctggaag aaggaatgaa
     5941 gatcaatgtg attgggcgtg tacaggttta tgaaccaagt ggtagctact ccatcatcat
     6001 tgaaaaagct gagcctgatg gggttggggc gcttgcgatt cagtttgaac aacttaagaa
     6061 aaaattgaca gaagaaggcc tgtttcaaga acgcttcaag caagctctgc cccaattttc
     6121 taagagaatt ggggtagtaa ccagccgtag tggagccgtt attcgagata ttatcacgac
     6181 cgtcagcagg cgattcccag gtgttgacat ccttctttat ccgaccaagg ttcaaggtga
     6241 aggggctgcg gaggaaattg ctagaaatat tgcgcgtgct aatcaacggg atgatttgga
     6301 tttgctcatt attggtcgtg gaggtggttc tattgaggat ctctgggcct ttaacgaaga
     6361 aattgtggta cgagctattt ttgaatctcg tttgccagtt atctctagtg tggggcatga
     6421 gacggatgtg accttggcag attttgtggc agatcgacgc gctgcaacgc caacagcggc
     6481 ggctgaactg gcaacacctg tgaccaagtt ggatgtatta gctcatttgc aaaatcagga
     6541 aaaacggatg gcaacggcag tccgaaatgt tctatctaag aaacaagagg ctttgaaaaa
     6601 atgcagtcag tctgttatct ttagacaacc tgagcgcttg tatgacggtt atttgcaacg
     6661 tttggaccaa ctgcaactgc gtttgaaaca aagtttgcga actcggattt ctgataacaa
     6721 acaattagtt caagcaagaa ctcatcaatt agtacaatta tcacctgtta ccaaaatcca
     6781 acgctatcaa gaccgtttag gacagttgga caagctctta ggtagccaaa tggcgttagt
     6841 ttatgacgcc aaggttgctg aggccaagcg actttcggaa gctttgctca tgttggatac
     6901 tagccgaatc gtggcgcgtg gttatgctat tgtcaaaaaa gaagagtcca ttgtagattc
     6961 ggttgagagt ttgaagaaaa aagaccaagt aacgcttttg atgcgagatg gtcaagtaga
     7021 attagaggtt aaagatgtca aaacaaaaga aatttgagga aaatctagca gaactggaaa
     7081 ccattgtcca aagtttggaa aatggtgaaa ttgctctgga agatgcgatt actgcctttc
     7141 aaaagggcat ggtcttgtca aaagagctcc aagctacgct ggacaaggct gaaaagacct
     7201 tggtcaaggt catgcaagaa gacggaacag aaagtgattt tgaatgaaaa agcaagaaaa
     7261 attagctctt gtcgagtcgg ctttggaaga tttttatgga gaccagcagt ttgcctctag
     7321 tttacgggag tctgttctct attctattca tgctggtggc aagcgtattc ggccttttct
     7381 cttgttagaa gttctggaat ccttgcaggt tgccatcaaa tctgctcacg cgcaggtagc
     7441 tactgccttg gagatgattc atacagggag cttgattcac gatgaccttc ctgctatgga
     7501 tgatgacgat tatcgaagag ggcggttaac caatcacaag aaattcggtg aagctatggc
     7561 cattttggct ggagatgcct tattcttaga cccatatgcc ttgattgcgc aggcagattt
     7621 gccaagtcag atcaaggtgg acttaattgc caacttatcc cttgcttcag gtagtctggg
     7681 tatggtggca gggcaagttt tggatatgga gggcgaacac cagcacttgt ctctggaaga
     7741 acttcagact attcatgcca ataagactgg gaagttacta gcctatccct tccaagcggc
     7801 agctattata gctgaattgt cacctgaaat gcaggtgaag ctgaaaactg tgggtgaatt
     7861 gattggactt gcttttcaag tcagagatga tgtactggat gtgacagcta gttttgagga
     7921 aatcggcaag acacctcaaa aggatctgca ggcagaaaaa tcaacctatc ctgccttgtt
     7981 gggcttggaa gagtccattg ccttttgtaa ccaaaccctg gatcaagcta atgaaaaatt
     8041 agaagaaatt gcccagcagc ttccctttga aacagaatcg attgtaagtg tagtagaaag
     8101 tttgagaatc aatggctaag gaaagagtgg atgtactagc ttataaacag gggttgtttg
     8161 aaacgagaga gcaggccaag cgaggtgtga tggctggtct agtcgtagca gtccttaatg
     8221 gagaacggtt tgacaagcca ggagagaaaa ttccagatga caccgaatta aaactcaagg
     8281 gggagaaact caagtatgtc agccgtggtg gtttgaaact ggaaaaggcc ttgcaggtct
     8341 ttgatttgtc ggtggatggc gcgactacga ttgatatcgg ggcctctact ggaggtttta
     8401 ccgatgtcat gctacagaat agtgccaagt tggtctttgc agtcgatgtt ggtaccaatc
     8461 agttggcttg gaaattacgc caagacccac gagttgtcag catggagcag ttcaattttc
     8521 gctatgctga aaagactgat ttcgagcagg agccgagctt tgccagtatt gatgtgagtt
     8581 tcatttccct tagtctgatt ttgccagcct tgcaccgtgt cttggctgat caaggtcagg
     8641 tggtagcact tgtcaaacct cagtttgagg caggacgtga gcagattggg aaaaatggaa
     8701 ttattcgaga tgctaagatt catcagaatg tccttgaatc tgtaacagct atggcagtag
     8761 aggcaggttt ttcagtcctt ggcttggact tttctcccat ccaaggtgga catggaaata
     8821 ttgaattttt agcgtatttg aaaaaagaaa agtcagcaag caatcagatt cttgctgaga
     8881 ttaaagaagc agtagagagg gcgcatagtc aatttaaaaa tgaataaaaa agaaagactt
     8941 gaaaaaatta gaagattggt gacagattat caaatcggca cgcaagaaga aattgtagaa
     9001 catttgaaag aagcaggtat cactgccact caggcgacgg tatcccgaga tatcaaagag
     9061 ttaggtattg tcaaaattcc tttgagagac aacacctatg tctacgaatt gccaaaatca
     9121 atcgtaaaaa gtctgcaact ggctgaagac aatatcgaat cggctgaatt gatggataag
     9181 atgatcaatc tccaagttat tccaggaaat acggcttttg taaaagctca gttaatcgag
     9241 acttttgcag acaagatttt tagctgtttg actgatgata gctcgatttt agtcattgcc
     9301 agaagtggaa gtctagcaga ggaaatcttt gaacaagtaa aaaattggta ggtctatatg
     9361 ttacttgaaa tttcgataaa aaactttgcc attattgagg ctatttccct caattttgaa
     9421 aaggggatga ctgtcctgac tggtgaaacg ggtgcaggga agtcgattat cattgatgcc
     9481 atgaatatga tgttgggagc tcgtgctacg acagatgtta ttcgtcatgg tgcaccaaag
     9541 gcagagattg aggggctttt ctcagttgag aatagtcgtc ttttacagga aatttttgat
     9601 gagcaaggtt tagaattagg tgatgaaatt atcatccgtc gggaaatctt gcaaaatggt
     9661 cgtagtatca gccgtgtaaa tggtcagatg gttaatctgt ctgttttgcg aactattggg
     9721 caacatcttg tagatattca tggtcagcat gaccatgagg agttaatgcg tccccaactg
     9781 catatccaga tgttggatga atttggtgat gccgcttttt tggacttgaa agaaacctat
     9841 caaacgagtt ttgatgccta tcggaaaatg cgcaagcagg ttctggaagt caagaaaaac
     9901 caacaggaac acaaggcacg tatcgaaatg ttggaatttc aaatggcaga gattgaggca
     9961 gcaaacttgc aggctggaga agacttggat ctcaatcaag agcgagataa actcctcaac
    10021 cacaaaaata ttgcggatac actgaccaat gcctacagta tgttggacaa tgaagatttt
    10081 tcaagtctgg ccaatgttcg ttcagccatg aatgacatgg aaagtgtcga agagtatgac
    10141 cctgaatacc gtgaaatttc aagctctctg tctgagacct actatgtttt agaagacatt
    10201 agcaaacgtt tggaagctat cattgaggac cttgattttg atggcaatcg cctcatgcag
    10261 gttgagaatc gtttggacct ccttcatact attactcgta agtatggtgg gactgttgat
    10321 gatgttttgc tttattttgc caagattacg gaagaataca atctcttgac aggcaataat
    10381 ctttcgtctg aggacatgga agcagagctt aagaagttgg aagtcaatct tgtcaatttg
    10441 gcaggtcaac ttgcttctgc tcgtcataat ttggctcagc aactcgaagc tgagattaaa
    10501 caagaactgc aagatcttta tatggaaaaa gcccagtttc aggttcgttt tagtaaggga
    10561 aaattcagtc gtgagggaaa tgaaatggtt gagttttata tttcaaccaa ccctggagaa
    10621 gactttaaac ccttggttaa ggttgcttct ggaggggaat tatctcgtct catgttagcc
    10681 attaagtctg ccttttcacg taaagaaggc aagactagca ttgtctttga tgaagtggat
    10741 acgggagttt caggtcgtgt tgctcaagct attgctcaga aaattcataa aattggtcag
    10801 catggtcagg ttttggctat ctcccatttg ccacaagtaa ttgcgattgc agattatcaa
    10861 ttctttattg agaagattag taatgaccat tcaacggttt cgactgttcg tctcttgacg
    10921 gtcgaagagc gagtggagga agttgccaag atgttggcag gtgatgatgt gacagaagca
    10981 gccctgacgc aagccagaga attgttgaga aacagggaga aataagatga cagattatta
    11041 tgtaattgga gatgttcacg gaaaagctgg gatgctggaa gaccttctta aaacctggga
    11101 tggtcagacc cagttgctct ttctaggtga tttgattgac agaggtgagg atagtcaccg
    11161 tgttcttgaa atggtcaagg acttggtgga caatcaaggg gctatctgtt tgtctggaaa
    11221 ccacgagtat atgtttttga cttggctaga tgacccagaa gaaagttatg accattatcg
    11281 tcgcaatggt ggagatacaa ccattaactc tatcctaggt cgtcccttgg atgcaccagt
    11341 tgatggagta gaagatgcca agcgggttgc cgctgaagcg gcagacttgg tcgaattcat
    11401 tcgtcaaatg ccatttgtag tagagacaga caagtatatc tttgttcacg caggtattga
    11461 tttgaccttg gatgactggc atgaaacaac agattataaa aaagtatggc ttagaaaacc
    11521 attccacgga gctgaaaatc atactggaaa aaccattgtc ttcggtcata caccggtcta
    11581 tggtttgtta cagcaagaac gcggtacatc tgagctttgg actacagaag atggcaagat
    11641 tggaatggat ggaggagctg tttatggtgg tgtccttcac gggattgtct ttacagacca
    11701 aggaatgaca gaacaccact ttatcgaaaa tgacggcttt gtcgctgaag attagtactc
    11761 ctagcagggt atggtcttgt caaaatatca aaaacaattt ataataaata gataccctga
    11821 aaggaagaga atcatgaact tagaagaatt gaaaaaacga caggagaaga tccgtaactt
    11881 ctctattatc gcccatattg accatggaaa atcaactcta gcagaccgca ttttggaaaa
    11941 aacagagacg gtttcaagtc gtgaaatgca ggcccagctt ttggatagca tggagctaga
    12001 gcgggaacgt ggaattacta ttaagttgaa tgccatcgag ttgaattaca ctgcaaaaga
    12061 tggggaaact tatattttcc atttgattga cacaccaggg cacgttgact ttacctatga
    12121 agtttcacgt tcgctagctg cctgtgaggg tgctattttg gtggtcgatg cggctcaagg
    12181 aattgaggct caaactcttg ccaacgttta tctggctttg gataatgatt tggaaatcat
    12241 gcctatcatt aataaaattg acctgccggc tgcagatccg gagcgcgtgc gtacagagat
    12301 tgaagatgtg attggtttgg atgccagtga agcagttttg gcttctgcca aggctggtat
    12361 tgggattgaa gaaatcctcg agcaaattgt agaaaaagta ccagcaccaa cgggtgatgt
    12421 gacggcgcca cttaaggcct tgattttcga ctctgtttac gatgcttacc gtggggttat
    12481 cctccaagtg cgtgtcatgg acggagtggt caaacctggt gataagattc agctcatgag
    12541 caatagtaag acctttgatg tggccgaagt cggtattttt acaccaaaag cggttggtcg
    12601 tgatttcctt gcgactggtg atgttggtta cattgcggcg tctatcaaga cggttcagga
    12661 tactcgtgtg ggtgataccg ttaccttggc aaccaatcct gcggcagaac cattacatgg
    12721 ttataagcag atgaatccta tggtctttgc gggtctctac ccaatcgaat caaacaaata
    12781 caatgacctg cgtgaagcgc ttgaaaaatt gcaactgaat gatgctagtc ttcagtttga
    12841 accagaaaca tctcaggcac ttggatttgg tttccgttgt ggatttcttg gacttctcca
    12901 tatggatgtt atccaggaac gtttagagcg tgagttcaat attgacctca tcatgacagc
    12961 tccgtctgtt atttacaaag ttaatttgac cgacggtgag tctatggatg tgtctaaccc
    13021 atctgagttt ccagacccaa ctaagattgc gaccattgaa gagccttatg tcaaggcgca
    13081 aatcatggta ccacaggagt tcgtcggagc agtaatggag ctagctcagc gtaagcgtgg
    13141 ggactttgtg actatggact atattgatga taaccgtgtc aatgttatct atcaaattcc
    13201 tcttgctgaa attgtctttg acttctttga taaacttaag tcttcgacac gtggttatgc
    13261 aagctttgac tacgaattgt cagaatatcg cccatctaag ctggtgaaaa tggatatcct
    13321 tctcaatgga gataaggtgg atgccctcag ctttatcgtt cacaaggact ttgcctacga
    13381 acgtgggaaa ctcatcgttg ataaactcaa gaaaatcatc cctcgccaac aatttgaagt
    13441 tccaatccaa gcggctattg gacacaagat tgtcgctcgt actgatatca aggcccttcg
    13501 taagaacgta cttgctaaat gttatggtgg tgacgtttct cgtaagcgta aactccttga
    13561 aaaacaaaaa gctggtaaga aacgcatgaa atccatcgga tcagttgaag ttccgcaaga
    13621 agccttcctc agcgtcttga gtatggatga agaataagtt atcagaaatg cctttccttg
    13681 ttcacaggaa atttatataa gaatcaatag taaagcaggc ttagatggtc tgctttttta
    13741 gtttttatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac tgtatgtatg
    13801 gttactgact tcgtcagttt catctacaac ctcaaaactg tgttttgagt aacctgcggc
    13861 ta
//
LOCUS       DAVMEF010000045        13536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMEF010000045 DAVMEF010000000
VERSION     DAVMEF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.045"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(550..1530)
                     /locus_tag="VYQ30_000930"
     CDS             complement(550..1530)
                     /locus_tag="VYQ30_000930"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1966070.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLFTSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLFGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1532..3784)
                     /gene="pbpX"
                     /locus_tag="VYQ30_000931"
     CDS             complement(1532..3784)
                     /gene="pbpX"
                     /locus_tag="VYQ30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1966071.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTVIKD
                     IKKITLTLGD"
     gene            complement(3788..4105)
                     /gene="ftsL"
                     /locus_tag="VYQ30_000932"
     CDS             complement(3788..4105)
                     /gene="ftsL"
                     /locus_tag="VYQ30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1966072.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(4117..5067)
                     /gene="rsmH"
                     /locus_tag="VYQ30_000933"
     CDS             complement(4117..5067)
                     /gene="rsmH"
                     /locus_tag="VYQ30_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1966073.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPRMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            5233..5427
                     /locus_tag="VYQ30_000934"
     CDS             5233..5427
                     /locus_tag="VYQ30_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1966074.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEDDF"
     gene            5443..5979
                     /locus_tag="VYQ30_000935"
     CDS             5443..5979
                     /locus_tag="VYQ30_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1966075.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSLLNTKHIFKTILGAFFFGV
                     MIHIVDSLRIARAKKDQD"
     gene            5990..6325
                     /locus_tag="VYQ30_000936"
     CDS             5990..6325
                     /locus_tag="VYQ30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966076.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLIPFPQLLTNPVQWKFSIFLAVTIISSLTYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6435..7436)
                     /locus_tag="VYQ30_000937"
     CDS             complement(6435..7436)
                     /locus_tag="VYQ30_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1966077.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7496..9397)
                     /locus_tag="VYQ30_000938"
     CDS             complement(7496..9397)
                     /locus_tag="VYQ30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1966078.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9419..9712)
                     /locus_tag="VYQ30_000939"
     CDS             complement(9419..9712)
                     /locus_tag="VYQ30_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1966079.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9712..10530)
                     /locus_tag="VYQ30_000940"
     CDS             complement(9712..10530)
                     /locus_tag="VYQ30_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1966080.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEVFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(10517..11296)
                     /locus_tag="VYQ30_000941"
     CDS             complement(10517..11296)
                     /locus_tag="VYQ30_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1966081.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(11311..11802)
                     /locus_tag="VYQ30_000942"
     CDS             complement(11311..11802)
                     /locus_tag="VYQ30_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1966082.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(11813..13003)
                     /locus_tag="VYQ30_000943"
     CDS             complement(11813..13003)
                     /locus_tag="VYQ30_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1966083.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(13015..13449)
                     /locus_tag="VYQ30_000944"
     CDS             complement(13015..13449)
                     /locus_tag="VYQ30_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1966084.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFELGI"
BASE COUNT         4003 a         3074 c         2352 g         4107 t
ORIGIN      
        1 ttgttttaaa aaatttagac tagttaaacc gctaccctac gtactatttc tggtttttct
       61 ttttcaagca ggagtagacc atctttactt ctgaagattt ctagatgaaa catagaccca
      121 cctctttcta ttttacttta aataagtgtt ctagtaaggc tttcatttac cttacgttca
      181 taatatacta cattggtcag aaaaggtcaa tagttttgac tttaactgac caatattttt
      241 ttacactctt cgaaaacctc ttcaaaccac atcagcttcg ccttatctac aacctcaaaa
      301 ccgtgttttg agctgacttc gtcagttcta tcaaccatct caaaactgta tattaaaatg
      361 aaataagaac aggataaatc gttcaggaca gtcaaatcga tttctaacaa tgttttagaa
      421 gtagaggtgt actattctag tttcaaacta ctatgttttg agcagcctaa agttagcttt
      481 ctagtttgtt ctttgatttt cattgtgtat ttaaacacaa aaacaccctg aaacatcagg
      541 gtgccattct tacatcaaat ataaaattgc gagggtcagg agacttgcta gaagtcccac
      601 tccccaaaag aagaagtcaa ccttccactc gctccaagga tttcctttac cagacaatcc
      661 cccaagctca aaatggtgat gtacaggcgt catacggaaa atacgtttac cacctgtcag
      721 tttgaaataa ctgacttgca tcataacaga agttgtttca aaaacataca caattccgat
      781 aatcaagaga gtccattctt ggtggagagc catagagata gctgccagca ttccacctaa
      841 agccaaactt cccacatcac ccataaagac cttggcaggc ttatggttaa agatgaagaa
      901 accgaacaaa ccaccaatca tggcaagaat cactagaaga atatccatct gaccttgcac
      961 ataggcaata actccatagg cagacaaact aatcacaacg gaaatactag ctaaaccgtc
     1021 aacaccgtct gtcaagttta ctgcgtttga aaaaccgact agccagaaaa gagcgaagac
     1081 aatatagaaa atccctagat gcacttgata accaaagaca gaaagcatat cgccaccgcg
     1141 ctcatagaaa agatagaaga taactccacc tagaagctga agagctaatt tttgcttagg
     1201 attaagcccc tcattgattt tacgaaagac cttgagaaag tcatctaaaa atccgaccaa
     1261 gccatacaag accaagatga acaaaatcat tcccatatta ttgctgaatt ggctactaaa
     1321 tagggcgaaa aagaaagcaa ccaaaacaga agtaaacaag aaaaccaaac ctcccattgt
     1381 aggagtccca gcttttgcct gatgctgttt gacatcctca tgcatctgct ggcctgtaat
     1441 ttgcgccttt ctataaaatt ggataaaggc cggaattcct actaaagtta gtaaaaatgt
     1501 cacaattcca gcactgatgg aaataaacat attagtctcc taaagttaat gtaatttttt
     1561 taatgtcctt gataactgtg ttagcacgaa catcttgctt ctgcacagta gagcccgagc
     1621 cttgaaattc aagttctata ttgagccact tagcaagggt ctcagcagtc tcctttgtcc
     1681 aaccatacat atctggaacc tcctctgctt tatcagataa gataaggact tgctggttcg
     1741 gggcaagatt cttcccttct tcagcagaac tgtttttaat cttcgttcct gttcccacaa
     1801 cgatgggttg tacaagattg cgacgcaatt cttctgctaa atcaccaggt gaaatatcct
     1861 tgacactagg cataggataa ggactttgtt gacttacttg ctccaaagct ttagctgttg
     1921 tttgaagatt gagagagtct ttcatagctg aagcccgctc caagatagga ttggcaaatt
     1981 ctcccaactg aatacctgaa taatgttcag gttgttggac cgtcacatac aagataaaat
     2041 caggattttc agccggactc atcgatacag ccgagaaaat atagtcggtt accccgacta
     2101 aataaccacc atttttctcg tcagcaatct gagccgtacc agacttgagg gctacatttt
     2161 gcccaggaac agttacagtt ggcttgcctg tgctgtggtt atacatggtt ccataaaccg
     2221 gatccgtccc taccaaaacc atgttagtcc gagttagact agctgcatct ttagaaacag
     2281 gatttcccac aatttctttt tgagatttcc gagcagtttg atcatttgga tcataaatgg
     2341 cactaataaa tttaggctcc agcatgacac cgtcattagc aatagctgta aaggcacgaa
     2401 tcatttgtgt ctgggtcact gaaatccctt gtccaaatga gctttgcgca atgttgacaa
     2461 tattatccgc aggaagctga ccagcatact catccgtcaa accgaaacgg gtcggaactc
     2521 caaatttaaa acgattaaga taatcaagcc aggtagcatc tcccatcttt tgctcaagga
     2581 gagtcatccc aacgttactt gagtgtgcaa aaccttgaga aaaagtcatc attctgccac
     2641 cagtcaatcc ttcattaacg tcccaatctc gaatcgtggc atctgcaatt tttaactcac
     2701 tactattaaa gacttctcct cctggaaagg tattattatc aatagcagca gccaacatca
     2761 tcactttcat agtggaacct ggctcatagt tactttggta aaggatatca cgccaaacaa
     2821 agtcctctgt aatgccttct tttgtatctg catcaaaggt cggtcgttgc gttgttgcca
     2881 gaatttcccc tgttttagca ctgaccaaag tcgctgtcat gtactttcct tttaccttct
     2941 cttgaaaagc atccatctgg gtttccataa aggactggag ggggctggaa atggttgtat
     3001 aaacatcctt accgtccatc gttcgttggg aaacttgttc tgttccgggt acaatattac
     3061 ccagacgatc cttttcatag gtaataatgc cgtctgtccc tgcaagaata ctgttcaagg
     3121 aactctccat tccagaggtt cccagcaagc tcttgcttcc atcttcattt tcatggagct
     3181 gagctagacc gataaaacta gaagcaaatt gtccgtttgg gtaactacga ttgggactgg
     3241 ttgtaaaatc aatccccttg acctctgcag cttccaattc ttttttgata gacatcatat
     3301 tggcataggt aatcccattt ccctttgctc caaaggaaac ttgcttgaga ttaggttgcg
     3361 agagttgctc tcttacatag gattcttcca tgtccagata cttatgaaag acctctgcaa
     3421 ccttgttaaa ttgtgttttt tctacgtaaa gaatcttacc cgttgctgac ttatagttct
     3481 catcaatgac cgcatagaca ttataggagg ttgcatcctc agcaatcggg actccatttc
     3541 ggtcataaat agtcccacgt ttggcaggaa ctgtacgggt ggtttgatga accttcttag
     3601 cttccttcgc taaatctgtt ccaaagcgag tgcctgtccc aataatgacc gcaaaattga
     3661 ctaaaaaaat ggcaaaaaca aagacagata ataaactcag actttttcca actctgcgtc
     3721 tgttttcagc cggcgatttc cgatttttgg tcgcataacg gattactctt tttgtccact
     3781 tcatatctta ctccgctatt ctaatatttt cattgtttaa ttgcaaatcg tgtgaattgg
     3841 caatttcttt caaacgttct gcacgtaata gttcattgac ctcttgcttg gcatcgtcca
     3901 attcggtctt cttttcctct atctgcgcat tgatttttgt caaatcattc tgcacttgca
     3961 agagcttggt ctgcataaaa ataatactaa tggctacaat aagagtggtt acagcaatgg
     4021 aaaagtaaaa agctttttcc acacgcgaaa accgtttaag ttgcatctgt agtatttgac
     4081 ctgttttttc cattttttct gccatctttt tccctcttac ttgtgaattt ttctggccac
     4141 gcgcaacttg gctgagtgcg agcggttatt ggcttctaac tcttccgcac ttggcaagat
     4201 tggcttacgg gacaccaatt ccatcctggg cttgagatca tctgggatga aaggcaagcc
     4261 ttttggaact tcaactgttg aagcttcctt gaacaattgc ttggtcaagc ggtcttctaa
     4321 ggaatgaaag gtaatcactg aaattctacc atccagagcc aacatatcca tagcctgctg
     4381 gatggactca tctgccgccc ccagttcatc attgacttca attcgaatag cctggaaaat
     4441 ctgcttagca ggatgcccct tcttcttgag ttccttggca ggtttgacca acttgataat
     4501 ctctgctaac tcagtcgttg tctcaatcgg cttcacttca cgcgcttgct caatcttacg
     4561 cgcaatctgt ttagagaatt tgtcctctcc atacttgaag aaaatacgaa ccaagtcatg
     4621 atagtcataa ttgttcacca cttcataggc tgtcaggcta gcatcctgat tcatccgcat
     4681 gtccagtggc gcatcctttt tataagaaaa accacgctca cgctggtcta attgaggact
     4741 agacactccc aagtcataac aaattccatc aatttcctga acaccagctt cgcgcaaacg
     4801 tgcctgtaaa tgacggaagt tgtccttgat aaaggtcacc attcccttct caatgtaagg
     4861 tgccaagcgt ttttgcgcat tgtcaatggc attctgatcc tggtcaaagg catagagatg
     4921 gcctttttca cttaatttac ttaataaata ctcgctatgg cctgctccac ccaaagtcgc
     4981 atcaacgtag ataccgtcag gttttacgtc aagcatatca atcgtttcgt ggagtaagac
     5041 cgttacatga tgaaattctt ttgtcatatc ttatctattt taccacaaat ccgaccagct
     5101 tgcacttgtc agacaaatag gacaaggaga ctactatttc ttcaaaaaat atgtcaaaaa
     5161 tacttgacac tttatgttta aaagactata ataaagtcaa atatatacga cacaaatcag
     5221 aaaggagacc agatgaatcg tgtgaaagaa tttcgcaagg aactgggcat ttcccagctc
     5281 gagctcgcca aggatatcgg tgtctcgaga cagactatca atatgattga aaacgacaag
     5341 tacaatccaa ccttggaact ctgtctcaat ctcgcccgca gcctccaaac tgacctcaac
     5401 agtctctttt gggaggatga tttttaaaaa aggagcaaac tcatgaaaaa agaaaccttc
     5461 actgaaaaac tgatcaaacg cacatacggt atttctggtc cccttgacga atacaaacgg
     5521 cgtgaggccg atcgtattgg gaaccaagtc tttatcatcc tcttttatct gatgattttc
     5581 ggaaatctta ttccactcct tctggcctat aaataccctc aagaagtggc tctaatctat
     5641 cctcctctga ttttagtgat tgccctcatc gctgctggct atgtcaccta ccaaatgaaa
     5701 aaaacaggga ttacagctat tgatccagac atgctgagcg agaaagaaag taagcaacta
     5761 cattacccag gtctgaaagc aggttcgatt tatggaacag tgatattttt tataattcca
     5821 ctcattgata ccctaacaag tgaaaatcca aattttatca gttctcttct aaatacaaaa
     5881 catattttta aaactatact gggagctttc tttttcggag tgatgataca tattgtcgac
     5941 tcacttcgta ttgcaagagc taaaaaagac caggattagg aggtgcctta tgaaatcact
     6001 agctagacta ctgatcattc atgtttttat cagtattttc cttttcttcg cccttatttc
     6061 aggagctgtt tctcatacag ttttactact cctactcctc tttcttcctg cgctcaataa
     6121 aggacttgag aaaatacaat caaaacggat acctgtcctc aacgcagccc tcttctttct
     6181 cctcataccc tttccacaac ttttaaccaa ccctgtccaa tggaaatttt caatattcct
     6241 agccgtaacc atcatttcaa gtttgaccta cttctataac ttttatcaag tagttaaaga
     6301 agtagatcaa aaacagttga tttaagagct tgctcccata atttctcatt tcataggcaa
     6361 ataatcccac ctagcttgtt ctaggtggga ttattttatt tcttaacaga aattgcaagt
     6421 cattttcctt cattttagaa agtcgactct ttccaattca cactacaatc caagacttgt
     6481 tgcctttctt cttgattgcg gccttcaatc tgatcaatca aaatctttgt agcctgttgt
     6541 ccttcctcaa aggagggctg aaccagcgtc gaaacacttg gagaagaaaa gcaagtccac
     6601 tccgtattgt caaaaccaat caacccaact tgtggcaagt tataattcaa ctctttgata
     6661 acggtaaaga ctagaggtag ggcccaacag ttagggataa ataccagagt tttttcatcg
     6721 ggatcgattt ctttttgtaa aaattcctta atttgttcca aattcgtatg cttatcttca
     6781 atggttagac tggcgtgacg catattagca tctgttaaag catccacaaa accacttgcc
     6841 cgctcaatcc gagtactcaa acgactcgta tccgctgtaa tcaagagaaa atgttcataa
     6901 cctttttcga tacaggactg ggtcatgtca taaacggcat catagttatt ggttttaacc
     6961 cagctagtcc ggtgttcata gagctgacta tcaaaaaaga ccattttctt ctttttctca
     7021 tcgatgatac gagaatattt tcggaaatta gaggtcggct gaataataaa gccgtctact
     7081 cccaagagaa gcatgctttc aatataccgg tcctcactct cttggctgta attactattt
     7141 cctatcatta cctggtagcc attctggctg gcgatatcct caattccctt aacaatttgg
     7201 tttgagaaac tgttggtaat atcaccgatc aaaacaccga ttaattttgt tcgtttggag
     7261 tttaagctac gcgcaacaat gctcggtttg taatttgttt catgaataac tttttcaatc
     7321 ttttcacgtg tctcttggga cattttttca tatttcccgt ttaggtaaaa tgacacggtt
     7381 gttttcgagg tctgagccat ttccgcaatg tcttttatgg tcagtttctt ctccaatcta
     7441 aaacacctcc tactaatact tattataaca tattcactat tcaaaatgcc tatttctagc
     7501 actgcaagcg aatcattctc tcatttattt tatcatatac tagacatttt ccacgcattt
     7561 ttgttccgtc aacgaggcac aatttttcac ctacaccaat atcctcgctt aataacagaa
     7621 tcagcttgtc tgtttcgtca tttttcactt caaaagccag agcattttcc acttcacgat
     7681 catctgtctg gtagacagaa tgacggatga tttcaaaact ctcatgcgca atgatggtat
     7741 aatccagcat ctgattctca aagaattggc gcttggtgag tttgctactt ctttcaagct
     7801 cattgtattt aggagaaatg atggtatctt ccaaatcaaa atcaacttca ctccatagtc
     7861 tcaactgatt gattttccca tcttgatagg tcacatcctt gtcaaggata aactgagtca
     7921 acgcctcatg ctgaccttga cacctgatgt catccaccaa gagccagaca tcctctacca
     7981 acatgaggat ttttcttctg tgaagataag gcaaatcagg ttctgctgac caataagccc
     8041 cttcaatata atgcattccc tccctttctt tatggtgaca aaacagggag tgaggatagt
     8101 attcatattc ccaggatccc gtgattcttt ccggagcttt cccatctaca atgcaggtcg
     8161 aatgactcca agcactcttt aagagataac gttcatatat ctcccgataa gaataacgcc
     8221 cagcatctat gaaaataggt tggccttgat actgtaagca aaaactattc tcgtcactat
     8281 ggctatgggc acttcctagc ggaccatttt tgaaaaatag ataacgatgt tcatccttaa
     8341 tacagacatg tccagagtct tcaaagatca tggacttagg ctgccaagct ctcctttcaa
     8401 attcctgcag tcgcttgacc ttttctcgcc ccaggaacaa gaggctaagc aaatcaactt
     8461 taacatccag accgttaaga aggtcttcct tgttcaaaac cacagcagac aggctcaaaa
     8521 tttctgtcgt ttctgtagaa tcgctatcac caaaagccaa agttcgtcca tctaagcctg
     8581 tcatcatttg aatataggtc gccatctttt ccagcaactc ttggtaacta tcttgcaagt
     8641 ctggaagcaa gagacacaaa tccagcaagg ctttataaac ctctacatga tagagaatcg
     8701 actgttcaaa ctggcttcca tctcctaaaa tctgtgtctc aatttgctgt ttcaactcct
     8761 ctgaagcaaa atggtaagct tcttctaggt ccatcttatc tgaaaagaaa tgatagatag
     8821 caagcatcgg aattgtttgt aaaatccccc agttactaag ggtgtacttg gcgcgatagt
     8881 agcttttcat aaagtcaatc tgcttttcta gactgaccaa aattttctct agttctttct
     8941 cctctagcaa gtcaaatttc aagagaagca agagtagttt caaccaagta aaggaacgaa
     9001 tacccgtatc caatgttcta gtcatcaagg attgaggaga aaattctctc acctgctcaa
     9061 tccaatcaaa tagaaagaac ttgcactttt gaatatagtc cttatctcct tctaccagat
     9121 accctatcat aaactgcaag agatattctt gtcgattgag catataagac cattctggat
     9181 catcttcaaa tacttgatcc cataccatcg gctggatttg atggattttt gaacaaggct
     9241 ccatatccca aggactatca aacataaaac gattgtccat caagcgttca agggaactct
     9301 tgactttctc atagtctttt gaacagtggg acaagatata atcacgacat tggtttccat
     9361 cgactctttc aaaaaattgt cttctttctt ctttcattat ctattaccag aaaaagaact
     9421 acttaaaaag cagttctttt gtctttccca ttacactttc cttttctaca tagatgacca
     9481 caccttttgc aatctgcaag gagaccaagt catctttgat agaaatgatt tttccatgaa
     9541 ttccagacaa taacaacact tcatcaccag atgttaaaga agctaaatac tcttgtcgtt
     9601 gctccatctg tttgcgaagc aacttttgct gacgaataga atgaaagctt gacagtaaaa
     9661 ggggactcac tgccaagaca atcactattc cataaaacaa tgttgtatcc attaagctat
     9721 aatcttaagc cagcttccga taattccgat gataactgtt aaaataacga gtttatatgt
     9781 tgtccatttc ttttctttga tcaagtagta aactaaaagt gtaaataggg ctggtagaag
     9841 agctggagca accttatcaa gcattccctg aatacttacg atactttgtt tagcgtctgc
     9901 tttaacttcc cctgcagcaa aggtaatcgg caccataatc ttaacagatg tcgctgccaa
     9961 accagcaatt acggttacac cgataatatt ggcaatacga gaaatcgttg ccatctgttc
    10021 gcttagttta tcaatcacag ttgttcctag tttgtatcca tacagaccag ttgacaattt
    10081 aatcgctgtt aaaatcgtat tcatcgcaag gaagaacaag attggaccga caaccaagcc
    10141 ttcttgagca aacgaagctg cgatggttga gaacaatgga gctaaacaga attgagaaag
    10201 agaatcccca atacctgcca atggtcccat caaggccatc ttgatgctac gtgtttcttt
    10261 tgccggacgg ccattttcca acattacaag atgcaagctg gtaataaaag gcaggaagtg
    10321 tgggttggta ttatagaatt cacagttttc ttccaaggct tggtagaaaa cttcctgatc
    10381 ctctccatag tgttttttca aagcaggata catcacattg gcatatccca acccttgata
    10441 gttactatag ttaaatccat tttgacaaaa gaatgcccgc aaagacgttt taagataatc
    10501 acgttttgtt aatttgttag atccagtcat cgtgtgcttc ctcctctacc acatgatccg
    10561 ctgtttttgg cttgttataa aattcaatca aagcaaagat agtacctaca attgcaatac
    10621 caattgttgg gatgtttaga taagctgcac aaacatatcc caacaagaca aagggaatca
    10681 actctttctt agccatcact gacaagatca tcgcaaaacc gatagctggg agcattttac
    10741 cagcaactgt caaacctgta agtaataccg gtggaatgta gtctacgagt ttcaacaagg
    10801 tatccattga aagggcaccc aagcaaccca aggtaaatcc aataaaggca aacaaccaaa
    10861 ttgttgcatt tagagtgaac ttgaatttct tcaaattatg gtttttcaag tgcttcatcg
    10921 ccgtttcagg cgcaccagca cgtacagtgt aggcgaaagt ttgtaagaat tgaatcgcca
    10981 cagcaatcgg agtagagagg gcaagagccg cttctggact gactttacca gcactagtga
    11041 tagccatcaa ggtaccaaag ataccaggtc cgattgggtt tggtggaaca gtacctccag
    11101 caccaacacc gaatcccata taagccaatt caccaatagc tcccattgca agagcagtag
    11161 gcagatcacc tagaataatt ccgacaccaa atgacagaac aatacatcta ttggtgtaaa
    11221 ttcctaacag cattccacta aaacagaaag ctgtccataa tccaattaaa atcgcttgaa
    11281 atacattaat cgacataacg atctcctctt ttaaatatag tccataatgt tgacttgaac
    11341 agctccatca tttcctgttg gagttgtttt cgtattaaat gttacttgat gagtttggct
    11401 caattccttg agggccacct tgtcctcttc acccaggaag atggagcgtg tcacttgctc
    11461 tttaccagta gcattgtgaa tgttcccaat attgatttct ttgataggga caccaccttc
    11521 taccaaggtt aaagcatcct tcacattctt tacaacgata aagatcgttt gagcaggatt
    11581 agccttgtga atgatatcaa tcaccttttg caaagggaag aaacgcatgg caactgagtc
    11641 tggcacaact gttttcatca gagtttgttg catcttgtcc gtgcttactt cgtcattggc
    11701 aacaatgacc gtattacaac ctaggtattt tacccaaagt tgtccttgtc catgaatcaa
    11761 ccgttcatcg atacgggtca taataatatt tggcattgtc atatttctcc tcctaccaat
    11821 ataggttcca gtctttgtag aaacggataa gggcttctag gtaatagtag tcaccccaga
    11881 tattgccttc atccactcct ttacctgaat gccatgagta cacaccgtgg aggagacttg
    11941 tcccaccagg ggtaaattga tcatttgcat aatgttcgat caaggaacga agcatggcat
    12001 gcatagcatg tttataaata tctttgtcag catccacctc tgggagatgt tttagcattt
    12061 catgaatccc acagacggcg atagctgttg ctgaagaatc tcgtgattga tcactaccat
    12121 cattaaaaat caaatcccaa taggacacat gatcttttgg cagacgattc aagaaataat
    12181 tggtcacacc cttaaacaag tcaaagcagg actcgtcttt taagtgacga taagtcaaag
    12241 gaataccata gactccccat gattgaccac gtgcccagca tgaatcatca ctataccctt
    12301 gtctcgttac acctttaaag ggttgacctg tctcaggatc aaaatagaag gtgtggaagg
    12361 acgaagcgtc atcacggatt acattattag ctgaagcata gaaatggctt tctgcaatat
    12421 cgtagtattt ttgatcgcct gtttcttgat aagcaaagaa taagagttgg atattgagca
    12481 agcagtcgat aatcaaacgg taatgctctt tcttgcccaa gtctccccaa gcttgaataa
    12541 aaccaccttt ttcttgatag cgttcaatca acttatctgc agctttcaag gttgcttctc
    12601 tagcctctcc atctccattt atcttatatt cagccataca agacggtgtg tacaagaagc
    12661 cgagatcatg gtgatccaat tctactctct tattgacacg atccaggaaa gaaagaacat
    12721 ttttatgagc gatgttttta aatgcatcct gttgactgta ttcataagcc aaccacagtt
    12781 ctcctgtcca gaaaccattg gtccattccg tgttatccat gattggatag acattatcaa
    12841 aggtagctgg agtcgggaaa tcttccttga aatagtcaag gttgagttct aactgccgaa
    12901 taaccttatc gattgcctgg ccgacttctt ctttcgtcag aagtggtact tctaagaagc
    12961 gctcaggcga ttttattttt tcaatcgtaa ccttttttat cattctttta ccctttaaat
    13021 acccaattcg aaatcttctt cctcttcctc tgcatccgtt gaaaacaatt ctttaccatt
    13081 tacgactccg ccctgggccg ccactactga tttattaaca acctcatcaa agctatgagc
    13141 cattctagca aagactgctt ccattaacat ggctaagttc aaacccgaga gaacattcat
    13201 tgttttgact ggattttctc ccattatggt agaagaaacc ttgaatggcg atcctcccaa
    13261 gagatcactt aggattaaaa cttcttcttc atttgaaatt gcaagtaaga ttttttgctt
    13321 gagttcatct gctgacattc cttccacaaa gtcaatcgcc tccacatttt cttgattacc
    13381 tgcaatcaat tgtaaagaac tataaatccc tgtagcaaaa tgtccatgcc ctacaagtac
    13441 aattttcatc ttttctcctt tcttgtttag gtttaccggt ttactttata ttccccattg
    13501 taatccttta tttttatctt gtcaatagtt ttttta
//
LOCUS       DAVMEF010000046        13088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMEF010000046 DAVMEF010000000
VERSION     DAVMEF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.046"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..799
                     /locus_tag="VYQ30_000945"
     CDS             26..799
                     /locus_tag="VYQ30_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966085.1"
                     /translation="MLAACSGGAKKEGQAASKKEIIVATNSSPKPFNYEENGELTGYE
                     IEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAVNNISYTKERAEKYLYAAPT
                     VKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQLEDYNKQHSDNPTILNYTKA
                     NLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVIDLPSDQQPYVYPLLAKGQD
                     ELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK"
     gene            840..917
                     /locus_tag="VYQ30_000946"
                     /pseudo
     CDS             840..917
                     /locus_tag="VYQ30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1021..1851
                     /locus_tag="VYQ30_000947"
     CDS             1021..1851
                     /locus_tag="VYQ30_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966086.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2005..2859
                     /locus_tag="VYQ30_000948"
     CDS             2005..2859
                     /locus_tag="VYQ30_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1966087.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            2959..4332
                     /locus_tag="VYQ30_000949"
     CDS             2959..4332
                     /locus_tag="VYQ30_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1966088.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4325..5386
                     /locus_tag="VYQ30_000950"
     CDS             4325..5386
                     /locus_tag="VYQ30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1966089.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5388..6080
                     /locus_tag="VYQ30_000951"
     CDS             5388..6080
                     /locus_tag="VYQ30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966090.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6110..6679)
                     /locus_tag="VYQ30_000952"
     CDS             complement(6110..6679)
                     /locus_tag="VYQ30_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1966091.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6814..7428
                     /locus_tag="VYQ30_000953"
     CDS             6814..7428
                     /locus_tag="VYQ30_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1966092.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7430..9076
                     /locus_tag="VYQ30_000954"
     CDS             7430..9076
                     /locus_tag="VYQ30_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1966093.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9088..10374
                     /locus_tag="VYQ30_000955"
     CDS             9088..10374
                     /locus_tag="VYQ30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966094.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10426..10959
                     /locus_tag="VYQ30_000956"
     CDS             10426..10959
                     /locus_tag="VYQ30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966095.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDETMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11037..11507
                     /gene="nrdI"
                     /locus_tag="VYQ30_000957"
     CDS             11037..11507
                     /gene="nrdI"
                     /locus_tag="VYQ30_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1966096.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11800..13062)
                     /locus_tag="VYQ30_000958"
     CDS             complement(11800..13062)
                     /locus_tag="VYQ30_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1966097.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3943 a         2452 c         2975 g         3718 t
ORIGIN      
        1 tgccctaggg cttgttgctg caggtgtgct agcagcttgc tcaggtggtg ctaagaaaga
       61 aggacaagca gctagcaaga aagaaattat tgttgcaact aattcttcac caaaaccatt
      121 caactatgaa gaaaatggcg aattgactgg ttacgagatt gaagtcgttc gcgctatctt
      181 taaagactct gacaagtatg atgtcaagtt tgagaagaca gagtggtcag gtgtctttgc
      241 gggtcttgac gctgaccgtt accaaatggc tgttaacaac atcagctaca ctaaagaacg
      301 tgcggagaaa tacctttatg cagccccaac tgttaaagac cctaacgttc ttgtagtcaa
      361 gaaagacgat tctagcatca aatcgcttga tgatgttggt ggaaaatcaa cagaagttgt
      421 tcaaggaaca acatctgcta aacagttaga agactacaac aaacaacact cagataatcc
      481 aactatcctt aactatacta aagcaaactt acaacaaatc atgggccact tgagcgatgg
      541 tcagtttgac tacaagattt ttgataaaat cactgttgaa acagtcatca agaaccaagg
      601 tttggacaac ttgaaagtca tcgatcttcc aagcgaccaa caaccgtacg tttacccact
      661 tcttgctaaa ggtcaagatg agttgaaaac atttgtagac aaacgtatcc aagaactcta
      721 caaagacgga actcttgaaa aattgtctaa acaattcttc ggagacactt acctaccggc
      781 agaagctgat attaaataat ttcttgaaat catccattct gagtaagatg ggtgattttt
      841 cagttcgcta ctgcaatcta gtcggaacag ttctctttgg agtggtcttg attgccatct
      901 tgcgtttggt cttttaaatt acccataaaa aaaacttatc acagagatag atatcatata
      961 atggcgtaaa tgctcttttt ctgttaagat tataaggtat tctattttgg aggaaatgac
     1021 atgaaaaaaa tcgttaaata ctcatctctt gctgccctag gacttgttgc tgcaggtgtg
     1081 cttgcggctt gctcaggtgg tgctaagaaa gaaggagaag cagctagcaa gaaagaaatc
     1141 atcgttgcaa ccaatggatc accaaggcca tttatctatg aagaaaatgg cgaattgact
     1201 ggttacgaga ttgaagtcgt tcgcgctatc tttaaagatt ctgacaaata tgatgtcaag
     1261 tttgaaaaga cagaatggtc aggtgtcttt gctggtcttg acgctgaccg ttacaatatg
     1321 gctgtcaaca atcttagcta cactaaagaa cgtgcggaga aatacctcta tgctgcacca
     1381 attgcccaaa atcctaatgt ccttgtcgtg aagaaagatg actctagtat caagtctctc
     1441 gatgatatcg gtggaaaatc gacggaagtc gttcaagcca ctacatcagc taagcagtta
     1501 gaagcataca atgctgaaca cacggacaac ccaactatcc ttaactatac taaggcagac
     1561 ttccaacaaa tcatggtacg tttgagcgat ggacaatttg actacaagat ttttgataaa
     1621 atcggtgttg aaacagtgat caagaaccaa ggtttggaca acttgaaagt tatcgaactt
     1681 ccaagcgacc aacaaccgta cgtttaccca cttcttgctc aaggtcaaga tgagttgaaa
     1741 tcatttgtag acaaacgcat caaagaactt tataaagatg gaacgcttga aaaattgtct
     1801 aaacaattct tcggagatac ttatctacca gcagaggctg atattaaata atttcttgaa
     1861 atcatccatt ctgagtaagg tgggtgattt ctcagttttt agggatatag ttttaaacta
     1921 tatatctgat tatctaataa caatttgtga aatcaaacaa attgtgagat actagtacgg
     1981 tattattttt aaggagaaag aatcatgaaa atcaaaaaat ggcttggcct agcagccctt
     2041 gctacagtcg caggtttggc tcttgcagct tgcggaaact cagaaaagaa agcagacaat
     2101 gcaacaacta tcaaaatcgc aactgttaac cgtagcggtt ctgaagaaaa acgttgggac
     2161 aaaatccaag aattggttaa aaaagacgga attaccttgg aatttacaga gttcacagac
     2221 tactcacaac caaacaaagc aactgctgat ggcgaagtag atttgaacgc tttccaacac
     2281 tataacttct tgaacaactg gaacaaagaa aacggaaaag accttgtagc gattgcagat
     2341 acttacatct ctccaatccg cctttactca ggtttgaatg gaagtgccaa caagtacact
     2401 aaagtagaag acatcccagc aaacggagaa atcgctgtac cgaatgacgc tacaaacgaa
     2461 agccgtgcgc tttatttgct tcaatcagct ggcttgatta aattggatgt ttctggaact
     2521 gctcttgcaa cagttgccaa catcaaagaa aatccaaaga acttgaaaat cactgaattg
     2581 gacgctagcc aaacagctcg ttcattgtca tcagttgacg ctgccgttgt aaacaatacc
     2641 ttcgttacag aagcaaaatt ggactacaag aaagcacttt tcaaagaaca agctgatgaa
     2701 aactcaaaac aatggtacaa catcattgtt gcgaaaaaag attgggaaac atcacctaag
     2761 gctgatgcta tcaagaaagt aatcgcagct taccacacag atgacgtgaa aaaagttatc
     2821 gaagaaacat cagacggttt ggatcaacca gtttggtaat aagaaacagg gaggtgggag
     2881 agaaaattcc acctcttgct tttgtataga gtatagattg taaagaagac tattcgttca
     2941 tagaaaggta gagagaatat ggtttttcct agcgaacaag aacagattga aaaatttgaa
     3001 aaggatcatg tagtccaaca ttattttgag gttttgcgta ccttgatttc taagaaatca
     3061 gtctttgccc agcaggttgg actcaaggaa gtcgcaaatt atctgggtga gattttcaag
     3121 cgtgttggag ctgaagtgga gattgatgag agctatacag cgccctttgt catggcacat
     3181 ttcaagagtt cgcgtccaga tgccaagacc ttgattttct ataaccacta tgacactgtg
     3241 ccagcggatg gggatcaggt ctggacagag gatcctttta cgctttcggt ccgcaatggc
     3301 ttcatgtatg ggcgtggggt tgatgacgac aagggtcata tcacagctcg cttgagtgct
     3361 ttgagaaaat atatgcagca ccatgatgat ttacctgtca atatcagctt tatcatggag
     3421 ggagcggagg aatcggcttc aacagaccta gataagtatt tggaaaagca tgcagacaaa
     3481 ctccgtgggg cggatttgtt ggtctgggaa caagggacca aaaatgcctt ggaacagctg
     3541 gaaatttctg gtggcaataa ggggattgtg acctttgatg ccaaggtaaa aagcgctgat
     3601 gtggatatcc actcgagtta tggtggtgtt gtggaatcag ctccttggta tctcctccaa
     3661 gccttacagt ctcttcgtgc tgcggatggc cgtatcttgg ttgaaggctt gtacgaagaa
     3721 gtacaagagc ccaatgaacg agaaatggcc ttgctagaaa cttatggtca acgaaaccca
     3781 gaggaagtta gtcggattta tggattggag ttgcctctct tacaggagga tcggatggcc
     3841 tttctaaaac gtttcttttt cgagccagcg cttaatatcg aaggaatcca gtctggttat
     3901 caaggtcagg gtgttaagac tattttgcct gcagaagcca gtgccaagct agaggttcgt
     3961 ctggttccgg gtctagaacc gcatgatgtt ctggaaaaaa ttcggaaaca gctagacaaa
     4021 aatggctttg ataaggtaga attatactat accttgggag agatgagcta tcgaagcgat
     4081 atgagcgcac cagccattct caatgtgatc gagttggcca agaaattcta tccacagggc
     4141 gtttcagtct tgccgacgac agcggggaca ggacctatgc atacggtctt tgatgcccta
     4201 gaggtaccaa tggttgcatt cggtctagga aatgccaata gccgagacca cggtggagat
     4261 gaaaatgtgc gaatcgctga ttattacacc catatcgaat tagtagagga gctgattaga
     4321 agctatgagt agagatatta tcaagttaga tcagatcgat gtgacttttc accaaaagaa
     4381 gagaaccatc acagcggtta aggatgtgac cattcacatc caagaagggg atatctacgg
     4441 aatcgttgga tattctggag cagggaaatc aacccttgta cgggtgatta atctcttgca
     4501 aaaaccatct gcagggaaaa ttaccattga cgacgatgtg atttttgacg gcaaggtgac
     4561 cttgacggca gagcagttgc gtcgtaaacg tcaagatatc gggatgattt tccagcattt
     4621 taacctgatg agccaaaaga cagcagagga gaatgtagcc tttgccctta aacactctgg
     4681 actcagcaag gaagaaaaga aggctaaagt agctaagttg ttggacttgg ttggtttggc
     4741 agatcgtgct gaaaactacc cttcacaact atctggaggg caaaaacagc gtgtggcaat
     4801 tgcgcgtgcc ttggccaatg atccaaaaat cttgatttca gacgagtcaa cttctgccct
     4861 tgaccctaag acaaccaagc agattttggc cttgttgcaa gatttgaacc aaaaattagg
     4921 attgacagtt gtcttgatta cgcatgaaat gcagattgtc aaagacattg ccaaccgtgt
     4981 ggcggttatg caggatgggc atttgattga agagggaagt gtccttgaaa tcttctcaaa
     5041 ccctaaacaa cctttgactc aagactttat ctcaacagcc acaggtattg acgaagccat
     5101 ggtcaaaatc gagaagcaag aaatcgtgga acacttgtct gaaaacagtc tcttggtgca
     5161 actcaagtac gctggagctt caacagacga gccacttttg aatgaattgt acaagcatta
     5221 ccaagtaatg gctaatattc tctatgggaa tatcgaaatt ctcgatggta ctcctgttgg
     5281 agaattggtg gtggtcttgt caggtgaaaa agcagcgttg gcaggtgccc aagaagccat
     5341 tcgtcaagca ggtgtacaac taaaagtatt gaagggagta cagtaagatg gaatcattga
     5401 ttcaaaccta tttaccaaat gtctataaaa tgggttgggc tggtcaggca ggctggggaa
     5461 cggctatcta cttaactctt tatatgacag ttctttcctt cattatcgga ggcttcttgg
     5521 ggctagtggc aggtctcttt ctcgtcttga cagcgccagg tggtgtcttg gagaataaag
     5581 tcgtattctg gattttagac aaaattacct caatttttcg tgcggttccc tttatcatcc
     5641 tcttggcaat cttgtcacca ctttctcact tgattgttaa aacaagtatc gggccaaatg
     5701 cagcccttgt cccactttct tttgcagtct ttgccttctt tgcccgtcag gtgcaggttg
     5761 tcttggctga attggatggc ggtgtcattg aggcggctca agcgagcgga gcgactttct
     5821 gggacatcgt gggtgtttac ctatcagaag gtcttccaga tttgatccgt gtgacgactg
     5881 tgaccttgat ttcccttgtt ggggaaacag ctatggccgg tgcggttgga gctggtggta
     5941 tcggtaacgt agccatcgct tatggattta accgctacaa tcacgatgtg accatcttgg
     6001 caaccattat tatcattttg attatctttg caatccaatt cttgggagat ttcttgacta
     6061 agaaattgag ccataaataa aaaagagccg cgtggctctt tttaactgat cagattttct
     6121 gtgcaaattt tttactcaag gcttgtccaa tcaaggcacc cactagggct ccgatgacaa
     6181 tacttgcgat aaatagaagg acagttccag ggtttggagc gaccatgata cggtcgatat
     6241 attcttggga ttttcctctt gccagaagag tagccatata ggctttgggc gcaatccaca
     6301 taagcaagat tggtcctgtt gtactaaagg cgaaaataat gaaagaaagg aagttctttg
     6361 ttttgtcctt gtattttcct aaatgagcta cttcatctgc taggaggcca cagataattc
     6421 caggaaggaa ggaaccagca ccgtgtttag ttcccaagaa aaagaaggca atgacaaggc
     6481 cgatagtggt aatggctcca aagcgcggaa cttttgcgac taggatcata tagacgctac
     6541 cgccgacaag ggcagtaaag gcaggcgcat aaaacatgtt tccagtttgg tcaaagagat
     6601 tgcccaaaag gacaccaatc cccatgcaga ggaagtaaag aactgcaaaa agcagtgtag
     6661 ttaagataga tttcttcatg aattgtctcc tgataatttt ttcacaattc tcattgtacc
     6721 acggtttggt gggattgtaa atggatacta gaagtttttg aaaacgcttg aaatatgata
     6781 taatggtttc atagttattt ttaggggaat atcatgggta gatttttaga ctttgtcttt
     6841 aatcgtttct ttttagggat gattgcgaca gccttctttt ggctattaac tttagcagga
     6901 gggattatcc ttggtctagc gccggctagt gccaccttga tgagcttata tgcagaacat
     6961 ggttatagct ttcgggaata cagtttgaag gaggcttggt ctctttacaa gcaaaatttt
     7021 gtctcaagca acctgatttt ctatagcttt ttaggtgtgg gtctagtttt gacctatggt
     7081 ttgtatctct tggtgcaatt gcctcatcag accattgttc atttgattgc gacccttttg
     7141 aatgtcctag tagttgccct gatctttttg gcttatacag tatctttaaa attacaagtt
     7201 tattttgcct tgtcctatcg aaatagtctc aaattatcct tgattggcat ctttatgagt
     7261 ctagcagctg tggccaagat tctccttggg actgtgctac ttgtagcaat tggttactat
     7321 atgcctgccc tgctattttt tgtaggaatt gggatgtggc atttctttat cagtgatatg
     7381 ttggaacctg tctatgaaat catccatgaa aaattggcga caaaatagaa tgaagcagtt
     7441 ttggctacat acgcttctaa gaacctatag ttcagtgatg atcattatca ttgcgagttt
     7501 tgcaatctta ctctcttacg ctgactggga ttcacgtgaa aaggaagccc agagagtagc
     7561 ccagcgtgta actgctagaa cagtgagtga aattgaatat taccatagag agtcaaccca
     7621 gatagctcag gctttagttg aaaaccaagc tcgtattgag ggaatctata aatactttag
     7681 tcttagcatg ccagactatt tttactggca attagagcgg aaagcttcgc cttatatatc
     7741 agtctctctg tatgaaaatg ttgatgacct ctatgttcga aatgattttg taactggagt
     7801 ggccattgct tttcaagatt acaaggaagt ctatgtttct actaaagaca aacgtagtgg
     7861 agaaaaaatc agggctgagg atttcaaacc agcaggaaat agttttgcca ttccggtgtc
     7921 agatccagtg tcagatcaag atttaggagt gatttacatc tccttggatc ctgctgtttt
     7981 ataccatgcc attgataata ctagaggtca tactccgatg gcagtaacag tgacctcacc
     8041 ttttgatacg gagatttttc atattggtga gacagttgat aaggagagtg aaaattggct
     8101 agttggctta acttctcatg gatatcaggt tcaggtggca gttcctaaaa actttgtttt
     8161 acaaggaaca gtgactagct ctgctttgat tgtgggtttg agccttctct ttattgtcat
     8221 tctttatctg actttgaggc agacttttgc taattaccaa aagcaggtag tggatttagt
     8281 agaatccatt caagtcattg ctcaaggcga agaggggcgt cggattgaca tttccgagaa
     8341 agatcaggaa ttactcctaa tcgcggagac gaccaatgat atgttggatc gattggaaaa
     8401 gaatatccat gatatttacc agttagagct tagtcaaaaa gatgccaata tgcgagcctt
     8461 gcaggcgcaa atcaatcctc attttatgta taatacgctg gagttcttgc gcatgtatgc
     8521 agttatgcag agtcaagatg agttggcaga tatcatttat gaattcagta gtctcttgcg
     8581 taacaatatt tccgacgaaa gagagaccct cctcaaacag gaattagaat tttgccgtaa
     8641 atacagctat ctctgcatgg ttcgctatcc caagtccatt gcctatggtt tcaagataga
     8701 tccagagtta gagaatatga agattcccaa gtttaccttg caaccgctgg tagaaaacta
     8761 tttcgcgcat ggtgttgacc acaggcggac agataatgtg attagcatca aggctcttaa
     8821 acaggatggt tttgtggaaa ttttggtggt cgataatggt agaggaatgt cggctgaaaa
     8881 gttggcaaat atccgagaaa aattaagtca gagatatttt gaacaccaag ccagctacag
     8941 tgatcaaagg cagtctatcg ggattgtcaa tgtacacgag cgttttgtgc tctattttgg
     9001 agaccgctat gccattacta tagagtctgc agagcaagcc ggtgttcagt atcgtattac
     9061 aattcaagat gagtagaaag ggagaaaatg tataaagtat tattagtaga tgatgagtac
     9121 atggtgacag aaggtctgaa gcgtttgatt ccctttgata agtgggatat ggaggttgtc
     9181 gcaacagcca gtcatgccga tgaagctcta gaatatgttc aggaaaatcc tgtcgatgtc
     9241 atcatttccg atgtcaatat gccagacaaa acagggcttg atatgattcg ggagatgaaa
     9301 gagatcttac cagatgctgc ctatatcctg ctctcaggtt atcaggagtt tgattatgta
     9361 aaaagagcaa tgaaccttag tgtggtggac tatttggtca agcctgttga taaggtagag
     9421 ctgggaaatc tgctggagaa gattgcaggt cagctcggcg agagagggaa gaaaagtcag
     9481 actcttagtc aagaattaga cgaggctgga tttgttagtt atttagggga taaggagaat
     9541 tggtggatag gtctatccaa ggaaaaacaa ggttccttca ccattcccta ctatgtcttg
     9601 ggccaagact ggcagatttt catttctggc caacctctag atggtttagt cgttacacct
     9661 tttgaagctc cttatcaaga acactttgaa cgctggaagc tgaatgctga gaaaaccctc
     9721 ttttacggtt ctgtaaattt gcagcagtct gagagtctct ttgcctatta cgaaccgatt
     9781 tatagggtta tcattcaggg aaatctcaat caaatcgtag aagagttaaa tctcttggag
     9841 aaggtagttc ttgaaaatac gccgcgtgtt ccgattacta aacagctttt tattcagttt
     9901 gtcatggatg ttttccattt atttgaacat ctcaaagctg atgacctgac agacatcgtc
     9961 aaaaccattc atgctattca atccttcgat gaattggttt cttatatcaa ggaaactctg
    10021 atcagctttt tcggtcaata ccgtatgaat gaaaatgtgg tcagtgtgct ggaagtcatt
    10081 ggtcgtgatt accaaaaaga gctttccctc aaggatatca gtaaggccct ctttatcaat
    10141 cctgtctatc tagggcagtt gattaagcgt gaaaccgatt cgacctttgc agagttacta
    10201 aataaacaac gtattaaggc tgcccagcag ctcttgcttt caactagtga cagcatcgaa
    10261 gatatttgtt atgctgttgg ttacagtaac cttggatatt tctataaagt tttccgaaaa
    10321 ttgtgcggaa aatcgccaaa agcctaccga aaacaggtag aaactatact ataagatttg
    10381 tattccttta caaaaggtgc tataatatta tcaggaggtc ctatcatgaa aaagaaacca
    10441 atttatctat gggttttgct aatcttgtct gcccttattt cagttccgtc tctgtttgga
    10501 attgtgagtc ccttgcctag taaagaagcc cttcgtgctg ctcagaaaca agttgcaggg
    10561 gtcaatgctc agcaattaga agaccagctt aattatacct atagagtagc agaagcatct
    10621 cattctattt ttaatgttgc tttgattgtg ctatctacta ttttagtggt cgtagcgatt
    10681 gtcttccttg ttcgtaaaaa tttgcaatat gcaaactata cttacgttgg ctatgttttg
    10741 ttagctatta ttggttcgat ttatggctat gttggtttac aagacgctgt gcagttggtt
    10801 caagatgaga ccatgcgttt gacagtgagt attggttcaa aagctgttag cattttctat
    10861 atcgtcatca atgttctctt cctagccctt gtcttctata agatgtggcg acaacagaaa
    10921 gccttggcag aagaagagga aacagaagag cttgcctaag tattgacaaa aaagaactat
    10981 caagatacaa tcttggtagt tgttttttga ttatagataa aatcatggag gtacatatga
    11041 agacaatttc tttagtttat attagtttga gtggcaacac tgaaagtttt gtgacgcgct
    11101 tgaaagacta tctcttgtcc cagtacaaag ggattgaggt tcaaaagatt catatcaagg
    11161 atttagtcaa ggaaggcaaa aatttctatg aaatggacca tccctatgtc gcttttttgc
    11221 cgacctatct cgaaggtggg aatggcgtgg ataacggaga tgttgagatt ttgacgacac
    11281 cggtgggaga ttttattgcc tatggtaaca atgctagtaa gtgttttggt gtggttgggt
    11341 caggaaatcg taactttaat aaccaatact gcctaacagc caagcaatac agtcaacgct
    11401 ttggcttccc tgtattggct gactttgaaa tgcgaggcat gctggaagat atcaaacatg
    11461 tcgcagctat tatcgcagat ttgtatgagt tggaaaagga gaattaatac tcaatgaaaa
    11521 tcaaagagca aactaggaag ctagtcgcag gttgctcaaa gcacagcttt gaggttgcag
    11581 atagagctga cgtggtttga agagattctc gaatagtata aagttcttat aagaggtggt
    11641 tgaagttgtt caactgtttt ttgagtataa acagtcttta agaacgtaaa aaagcataag
    11701 ttcaggtctt caaaaccttg ttcttatgct ttttatcata gaaatatttg ctgaattttc
    11761 tcttgaaatg aaaccttatc ttcatctcgc gcttgtccct taccctgctt ttccgattgc
    11821 gagtgcgtag ataaagaaga tggcgaagcc aaagaggaaa atcaatcccg caatggcaag
    11881 gtgtttgagc caagaaggaa gatttttgtt ttcacgcgtt accaaggcgt aattcaaaag
    11941 agcaaagaaa ggtgttgtca ggaaagagcc aatcatggca aagcggagca tggttgaaac
    12001 ctgaccagcg aagaacttga taatgacgat accgatgata gcagtgatgg tcatccagat
    12061 gttcaaagat ttacgattgt cctctttttg actgattagc agtcggagag attcctgatt
    12121 aacgcgagaa tagccatcga taacagttat aactgttcca aagatacaga ggaaggcaat
    12181 aaaggtaatc aagtaacggg accattcgcc aagaacagag gcatacatgc ccacgaattg
    12241 agagatgtat ttggctgaag cagcttcaac cgcctgccct gtaggatact gaatcagtgc
    12301 tcccagtgcc acaaagaaga cggctaggat agctgttcca atataaccag tgttaaagtc
    12361 aaacagagcg tcttctgtgt taaagttgac ggtctttctc ttttcagctg accaaagtga
    12421 attgatggct gaaatttcaa taggagccgg catccatcct aggagggaga cgatgaaggg
    12481 cagagctgcc atttgccaag gtgtcttctc gacaaaatca gaactgtatt ctggatgctt
    12541 gaccgccgca atgataactg caagaacagt cgcaatggtt aaggcagaca taatccattt
    12601 gaccatgccg tctaaaagtt tgtagcctcc aaagagtagc atagcccaaa tgattgcaac
    12661 gagaatgagg gaccactgag taatgctaag tccaatcatt gggaaggcac tggcgatgat
    12721 agctgagcac agaatggcaa caccagccgt gttgaccata gccgaaaaga cattgaggat
    12781 aaagaaaatc cagagataga gttttccttt ttcggcataa ccttcaacca aagtctttcc
    12841 agtatcagct gtgtattcag caccaaaacg gaaaaatgga tatttaaaga cattggctaa
    12901 gatgaccaag agaagtagag accaaccgta agaaccgcca gcttgagttg aggatacaat
    12961 gtgggaacct ccaacagcgg cagttgccat taggattcca ggtcccattg cttttagctt
    13021 gcttgcccag gttgattgat ttaaggaaat agcttgcgac atgataaata ctcctttttg
    13081 aattttca
//
LOCUS       DAVMEF010000047        12867 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMEF010000047 DAVMEF010000000
VERSION     DAVMEF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.047"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..496
                     /locus_tag="VYQ30_000959"
     CDS             <1..496
                     /locus_tag="VYQ30_000959"
                     /inference="COORDINATES: protein motif:HMM:NF013652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase 8 family protein"
                     /protein_id="HEV1966098.1"
                     /translation="IALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSEWFLLM
                     RNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYLDSDII
                     VNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKINTKFARVD
                     CSTP"
     gene            426..1202
                     /locus_tag="VYQ30_000960"
     CDS             426..1202
                     /locus_tag="VYQ30_000960"
                     /inference="COORDINATES: protein motif:HMM:NF013652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HEV1966099.1"
                     /translation="MMQKNGEKKKSTQNLLELTAQHHEHVYGDQGVLNIYFDDQWLHL
                     DKEYNFMVGLDQFLHLKGNADWYQSNYYGNYEPKIVHYTTEFKPWTHLTLTRFRKLWW
                     FYYGLNWNDVLLSSDIVKRSFKELVGVPLYHTCIFTNSAAMESLEYLLSELPEVHFTI
                     LAHTNFAPSVVDLQRYLNLSLFPNFNSFNMKETLDKMDFYLDINHEGEIVNIIEEVCR
                     RNKPIFGFDNTSHDSKGRSQIFSSTEPNKMVEAIRQFIGE"
     gene            1207..1476
                     /locus_tag="VYQ30_000961"
     CDS             1207..1476
                     /locus_tag="VYQ30_000961"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966100.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDDSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGRTLLL"
     gene            1491..2081
                     /locus_tag="VYQ30_000962"
     CDS             1491..2081
                     /locus_tag="VYQ30_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEV1966101.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            2110..3120
                     /locus_tag="VYQ30_000963"
     CDS             2110..3120
                     /locus_tag="VYQ30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1966102.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIS
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDILIVPSEQMKERLIEEGLTTKKILIQGMWDHPHDL
                     SLYTPIFKKELFFAGSLERFPDLQNWSQDTLLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            3130..5583
                     /locus_tag="VYQ30_000964"
     CDS             3130..5583
                     /locus_tag="VYQ30_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEV1966103.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQYWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSIYLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLERSVDLYLDINYSHVVGTILEDMKILEKPILSFDTTEHGNTGQLVFKKDEVSVMVQ
                     AIKDYRRDGRFLSCYEGSDFHCLTFTNSQELQKIDYLVKNFPMVTFHIAAWTVMGPML
                     QELAKKYKNVKLYPEVKSDQFEELKSQMDVYLDINLHNSTSEVVKEISSLNKPMLAFY
                     TSQNGNHGQHLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATLFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGVTSRSGVGNDLFDKVKSVKRIICPSHNAYSRIEEIQEAVLQYAENRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQILVDLSKREV"
     gene            5580..6464
                     /locus_tag="VYQ30_000965"
     CDS             5580..6464
                     /locus_tag="VYQ30_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966104.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYIHVFDSDYYVKNYNSKLLMEELPLLERYDM
                     SFLTSWGILFKRELFQEVQFPFGQVCEDIGTNYQLFMQVEKVTYVNKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRRLEMLDYLLNGNKYN"
     gene            6489..7709
                     /locus_tag="VYQ30_000966"
     CDS             6489..7709
                     /locus_tag="VYQ30_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV1966105.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            7718..8914
                     /locus_tag="VYQ30_000967"
     CDS             7718..8914
                     /locus_tag="VYQ30_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1966106.1"
                     /translation="MRKSIVLAADNVYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNSGVLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            8914..9819
                     /locus_tag="VYQ30_000968"
     CDS             8914..9819
                     /locus_tag="VYQ30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966107.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIE
                     TLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFN
                     SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRS
                     GSIMRTEFDLSWARDIVEVFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLT
                     DTEEYKDICFRLKLFFDAEQRNGKS"
     gene            9876..11093
                     /gene="secY2"
                     /locus_tag="VYQ30_000969"
     CDS             9876..11093
                     /gene="secY2"
                     /locus_tag="VYQ30_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEV1966108.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            11103..12683
                     /gene="asp1"
                     /locus_tag="VYQ30_000970"
     CDS             11103..12683
                     /gene="asp1"
                     /locus_tag="VYQ30_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEV1966109.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIVDDRGFVSSIIF
                     FEDGQAAYQEYLNLKGEWQFRERLKEGGQVEINPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            12667..>12867
                     /locus_tag="VYQ30_000971"
     CDS             12667..>12867
                     /locus_tag="VYQ30_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV1966110.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALRDIIEK
                     EDINRFHAVLIEDGQYAKDLFSY"
BASE COUNT         4129 a         1804 c         2666 g         4268 t
ORIGIN      
        1 tattgcttta ggagctgata ttcattatct tgataaggta gaaaccgtta taaagtcagt
       61 ttcagttcat aatcacgaag tgaaatttta tgtgtttaat gatgatctgc ctagtgaatg
      121 gtttcttttg atgagaaatc gtctgaaggt tattggttct gagattatta atgtcaaaaa
      181 agcagatcac aatcttcgtg atttccactt gccaaatgca atcctgtcct atgcaacttt
      241 ctttaggtat ttcatagcag acgaagtgct tgaagatcgg gtactctact tagactcgga
      301 tatcattgtg aatgccaatt tggatgaatt attttttttg gatttgcaag gatacgctat
      361 cgcagctgtt caagattttg atcaagatgg ttggttaaca acatttaatt caggtatgtt
      421 attgattgat gcaaaaaaat ggcgagaaaa aaaaatcaac acaaaatttg ctagagttga
      481 ctgctcaaca ccatgaacat gtctatgggg accaaggtgt tctcaatata tatttcgatg
      541 accagtggct acatttggac aaagagtata atttcatggt tggtttagat cagtttttac
      601 atttaaaagg taatgctgac tggtaccaat ccaactatta tggaaattac gagcctaaga
      661 ttgtccatta tactacagag ttcaaaccgt ggactcattt aacacttaca cgttttcgta
      721 aactctggtg gttttattat ggattgaact ggaacgatgt gttgttaagt tcagatatcg
      781 ttaaacgctc ttttaaggag ttagtgggtg ttcccttata tcatacttgt atttttacaa
      841 atagtgccgc aatggagtcc ttggaatatc tcctatcgga attgccagaa gtgcacttta
      901 cgatcttggc gcatacaaat tttgctccct ctgttgttga tttacagaga tatctgaatc
      961 tttcactttt tccaaatttt aattctttta atatgaaaga aaccttggat aagatggatt
     1021 tctatcttga tattaaccat gaaggagaga ttgtcaacat tatagaagaa gtctgtaggc
     1081 gcaataagcc aatctttggt tttgataata ctagtcatga tagtaaagga agaagtcaaa
     1141 ttttttcttc aaccgaacca aacaaaatgg tagaggctat tagacaattt ataggagaat
     1201 aagtttatgg cagacgaact aattagtatt gtagttccaa tctacaatgt tgagaattat
     1261 ttgcgaatgt gtttggatag cattcagaat cagacgtatc aaaattttga gtgtttatta
     1321 atcaatgatg actctccaga tcattcatcc aaaatatgtg aagaatttgt agagaaagat
     1381 tctcgtttca aatattttga gaaagcaaac ggcggtcttt catcagctcg taacctaggt
     1441 attgaatgtt cgggggggcg tacattactt ttgtagactc tgatgattgg ttggaacatg
     1501 atgctttaga ccgattatat ggtgctttga aaaaggaaaa cgcagatatt agtatcgggc
     1561 gttataattc ttatgatgaa acacgctatg tgtatatgac ttatgttacg gatccagatg
     1621 attctctgga agtgatagaa ggtaaagcaa tcatggatag ggaaggtgtc gaagaagtca
     1681 gaaatgggaa ctggactgta gctgtcttga agttattcaa gagagagtta ctacaagatt
     1741 taccatttcc tataggaaaa attgcagagg atacttactg gacatggaag gtacttctaa
     1801 gagcttcgag gatagtctat ttgaatcgtt gtgtttactg gtaccgtgtt ggtttatctg
     1861 atactttatc gaatacatgg agtgaaaagc gtatgtatga tgaaattggg gctagggaag
     1921 aaaagatagc tattttagca agttcagact atgacttgac caatcatatt ttgatttata
     1981 aaaatagatt acaaagagtg atagcaaaat tagaagaaca aaatatgcag ttcacagaga
     2041 tttatagaag aatgatggaa aaattgtctt tacttccgta gatagtaata aaaaatgaga
     2101 tagcgtaata tgaaactaca tttaacaaat ttatacggca tggctggtga tagtacggtt
     2161 atcttagcgc aaaatgctgt tcaaaagata gccagtcaac tgggatttag agaggttggc
     2221 atctattttt acaatatttc ttcagatagt ccttctgaaa tgaataagcg tctggatggc
     2281 attatggcca gtatctctat tggggatatt ttagtctttc agtctccaac ctggaatggc
     2341 tttgagtttg atcgtctctt gtttgataag ctgaaggata tgcaggtgaa aattatttgt
     2401 tttatccatg atgttgttcc tctcatgttt gatagtaatt actatctcat gaaagactat
     2461 ctgtatatgt ataatctatc ggatattttg attgttccat ctgaacagat gaaagaacgc
     2521 ctgatagaag aaggcttgac aacaaagaag attctcattc aagggatgtg ggatcatcct
     2581 catgatttat ccttatacac tccgattttt aaaaaagaac ttttttttgc aggaagttta
     2641 gagcgtttcc cagacttgca aaattggtct caagacacac ttttgagagt tttttcaaat
     2701 aaaggagagg cgagttctag tgctagaaat ctgagtattg aaggatggaa aaaagatgag
     2761 gaactattgc tagaattatc aaaaggtgga tttggtcttg tctggggaac tcatcagaat
     2821 gatggagaaa gcaaccaata ctataccttg aatatatctc ataaggttag tacctatttg
     2881 acagcgggca ttccagtcat tgtaccaagt agcttgtcaa ctgctaaatt tatagtagat
     2941 caaggcttgg gctttatggc ggatagtctg gaagaggttc atgagatagt tgataaaatg
     3001 aatctacaag aatatcaaga aatgacgaat cgtatcaaga cctttagcta tttgttaaaa
     3061 gaggggtatt tcactaaaaa gttattggta gatgcaatct atcacttggg aattgattaa
     3121 gggaatgaaa tgaacaaaac aattgtacta gcaggggatc gcaattacac caggcagtta
     3181 gaaacaacga taaaatctat tttataccac aatcgagatg ttaagattta tattttgaat
     3241 caagatatca tgccagattg gtttcgcaaa ccacgaaaaa tagctcgcat gttagggagt
     3301 gagattatcg atgttaaact acctgaacaa actgtgtttc aatattggga aaagcaagat
     3361 cacattagta gcattaccta tgctagatat tttattgcag attatatcca agaagataag
     3421 gttttatatt tagacagtga tttgattgta aatacttctt tagagaaatt atttagtatt
     3481 tatttagaag aaaaatcact cgcagcagtt aaagatacag atggaattac atttaatgca
     3541 ggtgttttat taatcaacaa taaaaaatgg cgtcaagaga aattaaaaga acgactaatt
     3601 gaacagagca ttgttacaat gaaggaagtt gaagaaggcc gtttcgagca ttttaatggt
     3661 gatcaaacga tttttaatca ggtcttacaa gatgattggt tagagctagg tcgagcttat
     3721 aatttacaag tagggcatga tattgtggct ttgtataaca attggcagga acatctggct
     3781 tttaacgata aaccagtggt gattcatttt acgacctaca gaaaaccctg gaccaccttg
     3841 acagctaatc gttatcgtga tttatggtgg gaattccatg atttggagtg gagtcagatt
     3901 ttacaacacc atatgggaga atttgaacta atatcgcctc tagataagga attttcttgc
     3961 ttaaccttaa cgaattccca agatttagaa ggaatagaag agctagttac agctctacct
     4021 gaggtggtat ttcatatcgc agcttggacg gatatgggag ataaattaaa aaaattagct
     4081 gtatataata atgtgagatt gcatccacaa attgttcctc ctgtattaga taagctggag
     4141 agaagtgtag atttatatct ggacattaac tacagtcatg tggtaggtac tattttagag
     4201 gatatgaaga tcttagaaaa gccaattcta tcatttgata cgactgaaca tgggaatact
     4261 ggccaactag tattcaaaaa agatgaagtt tcagtcatgg ttcaagcgat taaagattac
     4321 agaagagatg gaagattcct aagttgttat gaaggtagtg atttccattg cctaacattt
     4381 acaaattctc aagaattgca aaagatagat tatcttgtta agaattttcc tatggtaaca
     4441 ttccatatag cggcttggac agttatggga ccaatgttac aagaactagc aaaaaaatat
     4501 aaaaatgtta agctatatcc tgaagtgaaa agcgatcaat ttgaagaact caaatctcaa
     4561 atggatgttt atttggatat taacctacac aattctacta gtgaggttgt caaggagata
     4621 tcatcattga ataaaccaat gctggcattt tatacttcac aaaatggaaa tcatggtcaa
     4681 catttatatt caagcgagca tccagaaagg cttcttcaag catttcatgc attgataaaa
     4741 ggagagactt tgggagaacc agagaagcca atcaaggtta tcggaattga tcagactctg
     4801 gattatgtta tcaaaaataa gtcctcatta gttcgttttg gagacggaga agtcaatttg
     4861 atgtggggtt tgcctatacc ttatcaaaat catgatctag agttagctaa ccaattaaaa
     4921 catattgtgg gtcttgagag tgacgagaaa ttagtggttt gtttacctga tgcttttgac
     4981 gatcgttttg tctttacttg gtgggctaca ctattctgga aagaacatat gaatgtttat
     5041 atggattttt ataaagagtt gtgtaagggt tcatggtatg gctctacctt tatctcgcgt
     5101 ccatacattg attatgaaga taagagtaag gcaaaaggac aatttgaaaa gctaaagagt
     5161 atttgggaga atagggatat tctgattgta gaaggagtaa cttcacgttc aggtgtaggt
     5221 aatgatttat ttgataaggt caaatctgtg aaacggatta tttgtccatc tcataatgct
     5281 tattcgcgta tagaagagat tcaagaagct gtattacagt atgctgaaaa tcgccttatt
     5341 ttatgtatgc tgggtcctac agcaaaagtt ctgagttata atctatgcca gatgggctat
     5401 caagttttgg atgtaggcca tattgactca gagtatgaat ggatgaaaat gggagctaaa
     5461 actaaggtta aattttctca taaacatact gcagaacata atttcgacca agatattgaa
     5521 tttattgatg atgaaaccta taacagtcag attttagttg atttatcaaa gagagaagta
     5581 tgaaagattt aataagtgtt atagtaccag tttataacgt agaaccattt atttctagct
     5641 gcttagacag tttatcgaaa cagatttatc aaaattttga agttttatta gtaaatgatg
     5701 gctctacaga taattcagga gcaatatgtc gagagtatgc tgatcgtgat agtcgttttc
     5761 actatttcga aaaagaaaat gctggggtag cggatgcacg taattttgga atcgagagat
     5821 ctaaaggaga ttatattact tttgtagact cagatgattg ggttactgaa gagtatttat
     5881 caattcttat agaaactttg aaggagcagc attctgaaat tgttgtttca acttattcaa
     5941 cctataatga gtcagatggc ttattctata ttcacgtatt tgactccgat tattatgtga
     6001 aaaattataa ttcaaaactt ctaatggaag aacttccttt attagagagg tatgatatga
     6061 gctttttaac ttcttgggga attctattta aaagggaatt atttcaagaa gttcagtttc
     6121 cgtttggtca ggtgtgtgag gatataggaa ccaattatca actatttatg caggttgaga
     6181 aagtaaccta tgttaataaa gttttgtatt ggtatagggt tgggaaagaa ggactttcta
     6241 attcttattc accgaagatg atgcgtgatg attgtgattt taggttagaa cgaattgccg
     6301 tgctagcgct aaaaggatat gatgtcagta aatatttaga ccagatgaag ttctatttga
     6361 aatatcgtca tgatattgca attcaacgag aacttaagga gaatgtggaa acaagacgtt
     6421 tagaaatgtt agactattta ttgaatggta ataaatataa ctgaaaatta agatagagag
     6481 acgataaaat gttacaagat aagaaatctg tagtatttgc aggtgattac gcttatattc
     6541 gacaaatcga aacggcgatg aagtcactct gtagacacaa tagtcatttg aaaatttatc
     6601 tgctaaatca ggacattcct caggaatggt ttagtcaaat aagaatatat ttacaagaga
     6661 tggggggcga cttgattgac tgcaagttaa ttggctcaca gtttcaaatg aattggtcta
     6721 ataaattacc tcatatcaat catatgacat ttgcacgcta ttttattcca gattttgtaa
     6781 cagaagataa agttctctat ctagatagtg atttgattgt gactggtgat ttgaccgatt
     6841 tgtttgaatt agacttaggt gaaaattatt tggcagcagc tcgttcttgc tttggagcag
     6901 gagtcggctt caatgctggt gttctcttga ttaacaacaa aaaatgggga tctgaaacta
     6961 ttcgacaaaa attgattgac ttaacagaaa aagaacatga gaatgtggaa gaaggagacc
     7021 agtcaatttt gaatatgttg tttaaagatc aatatagttc ccttgaagat caatataatt
     7081 ttcaaatagg atatgattat ggggcggcag cctttaaaca tcaattcatt tttgatattc
     7141 cgctcgaacc actgccacta attttacact atatttctca ggataagcct tggaatcaat
     7201 tttctgttgg acgtctaaga gaagtttggt gggaatactc tttgatggat tggtctgtta
     7261 ttttaaatga atggttttca aagagtgtga agtatcctag taaatcacaa atatttaagt
     7321 tgcaatgtgt taatttaacg aattcttggt gtgtcgagaa aatcgattat ttggcggagc
     7381 aattgccaga agttcatttt catattgttg cttatacaaa tatggcaaat gaactactag
     7441 ctttaacgcg ttttcctaat gttaccgtat atccaaattc cttaccaatg ttattggaac
     7501 aaatagtaat agcttcagat ttgtatttgg atttgaatca tgatcgaaaa ttagaagatg
     7561 catatgagtt tgtgcttaag tacaaaaaac caatgatagc tttcgacaat acttgctctg
     7621 aaaatctttc tgagatttca tatgaaggta tctatccaag ctccattccg aaaaaaatgg
     7681 ttgcagcaat cagatcttac atgaggtaga gaacagtatg agaaaatcaa tagtattagc
     7741 ggcagataat gtctatctta ttcctttaga gacgactata aagtctgtat tgtatcacaa
     7801 tagagatgtt gatttttata ttctcaacag tgatatagct cctgaatggt ttaaattatt
     7861 ggggagaaaa atggaagttg tgaattctac aattcgcagt gtacacattg ataaagaact
     7921 ttttgaaagc tataaaacag gacctcatat aaattatgct tcttacttta gattttttgc
     7981 gacagaagtg gttgaatctg atagggtatt gtatctggat tccgatatca ttgtaactgg
     8041 ggaactagct actttgtttg agatagatct caaaggatat tcaattggtg ctgttgatga
     8101 tgtctatgcc tatgaaggac gaaaatctgg atttaattct ggagttttat taatggatgt
     8161 tgcaaaatgg aaagaacact ctattgtcaa tagtttattg gaattagcgg ccgagcaaaa
     8221 tcaagttgtt catcttgggg atcagagtat tttaaatatt tattttgagg ataattggct
     8281 agccttagat aaaacatata attatatggt aggtgttgat atttatcacc ttgctcaaga
     8341 atgtgaacgt ctagatgaca atccacctac aattgttcac tatgctagtc atgataaacc
     8401 ttggaataca tatagtatat ctagactacg tgaattatgg tgggtttata gagatttgga
     8461 ttggtcagag attgcttttc aacgttccga tttaaattat tttgaaagaa gcaatcagtc
     8521 taaaaaacaa gtgatgcttg tgacatggag tgcagatata aaacatttag agtatttagt
     8581 acaacggtta cctgattggc attttcattt ggctgcaccg tgtgattgtt ctgaggagct
     8641 gacctctcta tcacagtata cgaatgtaac agtatatcaa aatgtattac atagtagaat
     8701 tgattggcta ttggacgatt ctatagttta tttagatatt aatacaggtg gagaggtttt
     8761 taatgtagtt acaagggcac aagaaagtgg caagaaaatc ttcgcttttg atatcacacg
     8821 taaaagtatg gatgatggac tctatgacgg tattttttct gtggagagac cagatgattt
     8881 agtggataga atgaagaata tagagataga gtaatgagtg aattaattag tgttgtggta
     8941 ccgatataca atacgggaaa atatttagtg gagtgtgtcg agcatattct gaagcaaacc
     9001 tatcaaaata tagaaattat tttagttgat gacggttcta cggataattc tggggaaatt
     9061 tgtgatgctt ttatgatgca agataatcgt gtgcgagtat tgcatcaaga aaataagggg
     9121 ggggcagcac aagctaaaaa tatggggatt agtgtagcta agggagagta catcacgatt
     9181 gttgattcag atgatatcgt aaaagaaaat atgattgaaa ctctttatca gcaagtccaa
     9241 gaaaaggatg cagatgttgt tatagggaat tactataatt atgacgaaag tgacgggaat
     9301 ttttattttt atgtaacagg gaaagatttt tgcgtcgaag aattagctat acaagaaatt
     9361 atgaaccgtc aagcaggaga ttggaaattc aatagctcgg cctttatatt gccgacattt
     9421 aagttgatta aaaaagaatt attcaatgaa gttcactttt caaatggtcg ccgctttgat
     9481 gatgaagcaa ctatgcatcg cttttatctt ttagcctcta aaatcgtctt tataaacgat
     9541 aatctctatc tgtatagaag acgttcagga agcatcatga gaacggaatt tgatctttcc
     9601 tgggcaagag atattgttga agtgttttct aagaaaatat cggattgtgt cttggctggt
     9661 ttggatgtct ccgttctgcg tattcgattt gtcaatcttt taaaagatta taagcaaact
     9721 ttagaatacc atcaattaac agatactgag gaatataaag atatttgttt cagattaaag
     9781 ttgttttttg atgcagaaca aagaaatggt aaaagttgaa ataaaagaat tgttatttac
     9841 catatcacaa acaatgaagg tgaggggagt gttttatgac taagatttat tcgtcaatag
     9901 cagtaaaaaa aggactattt accttatttc tactgtttat ctatgtattg ggaagtcgta
     9961 ttactctccc ttttgttgac ctaaatacta aagatttttt aggaggttca acagcctatc
    10021 tagccttctc agccgcccta acaggtggga atctaagaag tttatcaatt ttttctgttg
    10081 gattatcccc ttggatgtca gctatgattt tgtggcagat gttttcatat tctaaaaaat
    10141 tggggctaag ttctacagcg attgaaattc aagatcgtcg aagaatgtat ctgaccttgc
    10201 taattgctgt gattcaatcc ttggcagtta gcttgagctt gccagtacaa tcctcctact
    10261 cggtaatttt ggttgctcta atgaatacct tgttactcat agcaggaacg ttttttcttg
    10321 tttggttgtc agatttaaat gctagcatgg gaattggggg ttctattgta atcctcctat
    10381 ccagcattgt tttaaatatc cctcaggatg ttattgaaac ttttaaactg gtttatatcc
    10441 ctacagggat tgttgtcttg cttgttttta tgaccattat attttcttac ttgcttgccc
    10501 ttatgtatcg agctcgctat ttggttcctg ttaataaaat tggcttacac aatcgattta
    10561 aacgctattc ttatctcgaa atcatgttga atcctgcagg tgggatgcct tatatgtatg
    10621 tgatgagttt tcttagtgta ccagcttatt tgttcatctt gttgggattt attttcccta
    10681 atcattcagg gctagcggct ttatcaaagg aatttatggt tggaaagcct ttgtgggtct
    10741 atgtttatat ttcggtctta tttttattta gtatcatttt tgcttttgtt acgatgaatg
    10801 gagaagagat tgcagaccgt atgaaaaaat ctggagaata catttatggt atttatccag
    10861 gtgcggatac tagtcgattt attaatcgat tggtccttcg tttctcagtc ataggtggtc
    10921 tctttaatgt ggttatggca ggtggtccca tgctttttgt tttgtttgat gaaaagttat
    10981 tacgattggc aatgattcca ggtttgttta tgatgtttgg aggcatgatt tttacgatta
    11041 gagacgaggt caaggcttta aggctaaatg agacctataa acctttgatt taggagactt
    11101 ttatgtatta ttttattcca gcttggtatg ggtcagaaag aacatggcat gcagatatca
    11161 ctccatggta tttttctcat tttcgtctag agtttgatga tacctttcac cagattcggc
    11221 tctttcaaga gcaagatata gattctcgtc tattagtatt agcttaccag cctcatctac
    11281 gttatttttt atatagacat ggtgtgttag aaacggatac ttattccgtt tttgatgtta
    11341 tgcaagattt tcataatctc catacccaag ttttaagcat tagagatatt gagtgggatg
    11401 atgactgtga atttatttat agtcccttta cgattatcgt tcaaaaaaat gggaagaaat
    11461 ttgctaaggt tgaacatgga gttgaaggct tcatcagtga tatacagtat tttgaaccaa
    11521 atggtcaaat acatatgcac catatcgtgg atgatcgtgg gtttgtatcg agcattatct
    11581 tttttgaaga tgggcaagca gcctatcaag aatatctgaa cctcaaggga gagtggcaat
    11641 tcagagagcg tttaaaagaa ggaggacagg tagaaatcaa tccaattttg ggttatcgct
    11701 ttaaaatgct tacctatcaa aatatgggag atctggtggc agaatttttt gagaattatc
    11761 tgcaaacgta tgtgaaggat caggatattt ttatgcttcc ttctcattct catcatgacc
    11821 agttggtact agatcgttta cctagtacta atcctaaact gttgagtctg ttcattggac
    11881 gtaatcctca agataccttt agggatttag atgtaacttt tgaaaaatcg gatttgattt
    11941 tggtggatag agaggatagt ttacgattgt tgcaggagtt gtatcctgaa cgaatgcatc
    12001 aatgttatca tttatcatct tttgacaccc gattacgatt gggacgaagc caaactaaga
    12061 aagaatccat catttatttt caactggatt ttgagcaggg gattgataat caagctctgc
    12121 ttcaagtctt gtcctttgtc gctgaaaata aggatactga ggtgattttt ggagcctttg
    12181 ctgctagtca ggagcaaatg aatgaggttg aagggattgt tgagtctttc atccaagaaa
    12241 acattcaatc cgaaaatctg ggaaaggcga ttgattatgg tgatgcagaa aatcctctgg
    12301 aagaaaatca acaccaggac ttacgcttac agtttgttaa cttgaatgat gagttagatt
    12361 tgataaaaac actagaattt gtccgtttga ttgtggattt aaatagacat cctcatctct
    12421 acacacagat tgctgggatt agtgcaggaa ttcctcaaat caacctagtt gaaaccgtct
    12481 atgttgaaca tttaaaaaat ggttatttgt tagcagatgt tacagaattt tctaaggctg
    12541 cacattatta cacagatagg ttgaaggagt ggaatgagtc cttgatatat tcaattgata
    12601 agattaagga gcacacagga caacaatttc ttggaaaatt agagaaatgg atagaggagg
    12661 ttaaaaatgt caaaggaact taatatttta cagataggac ttgccaattg ggaaaatcac
    12721 tatgacatac ctgaaaatat gagttggtat tatttttacc caaactcatc aaaagctctt
    12781 cgtgacataa ttgaaaaaga ggatattaac cgttttcatg cagttttaat agaagatggt
    12841 cagtatgcta aagacttatt ttcctat
//
LOCUS       DAVMEF010000048        12657 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMEF010000048 DAVMEF010000000
VERSION     DAVMEF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.048"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(134..616)
                     /gene="greA"
                     /locus_tag="VYQ30_000972"
     CDS             complement(134..616)
                     /gene="greA"
                     /locus_tag="VYQ30_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1966111.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(687..2342)
                     /gene="mltG"
                     /locus_tag="VYQ30_000973"
     CDS             complement(687..2342)
                     /gene="mltG"
                     /locus_tag="VYQ30_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1966112.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPAIETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2423..2917)
                     /locus_tag="VYQ30_000974"
     CDS             complement(2423..2917)
                     /locus_tag="VYQ30_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966113.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2914..3378)
                     /locus_tag="VYQ30_000975"
     CDS             complement(2914..3378)
                     /locus_tag="VYQ30_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966114.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3388..4722)
                     /locus_tag="VYQ30_000976"
     CDS             complement(3388..4722)
                     /locus_tag="VYQ30_000976"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1966115.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAIYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4733..5350)
                     /locus_tag="VYQ30_000977"
     CDS             complement(4733..5350)
                     /locus_tag="VYQ30_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1966116.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5398..8496)
                     /locus_tag="VYQ30_000978"
     CDS             complement(5398..8496)
                     /locus_tag="VYQ30_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1966117.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDILLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFESDFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8589..9755)
                     /locus_tag="VYQ30_000979"
     CDS             complement(8589..9755)
                     /locus_tag="VYQ30_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1966118.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9763..10857)
                     /locus_tag="VYQ30_000980"
     CDS             complement(9763..10857)
                     /locus_tag="VYQ30_000980"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1966119.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDTRDLIADLRQALEG"
     gene            complement(11015..12136)
                     /locus_tag="VYQ30_000981"
     CDS             complement(11015..12136)
                     /locus_tag="VYQ30_000981"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966120.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETSFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIY
                     SSSKGSSVALENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKIAFVGPSGSGKSTIVRLLERFYKPLSGYILMEQSSIYDFNLKEWRSKIAWVSQNNA
                     VLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGR
                     SQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVK
                     DVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(12145..>12657)
                     /locus_tag="VYQ30_000982"
     CDS             complement(12145..>12657)
                     /locus_tag="VYQ30_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966121.1"
                     /translation="VIMGSFLLVLSSLLALLLPLILKGLIDGSSIENIGSKVFQSFLI
                     FIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDT
                     SVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISE
                     KYSRRLQEEI"
BASE COUNT         3601 a         2868 c         2356 g         3832 t
ORIGIN      
        1 atgaaactga caaagtcagt aaccatacat acggcaaggc gacgctgacg tggtttgaag
       61 agattttcga agaatagcaa aatggaaaaa ggagtgagtg aagcacatcg cctccccact
      121 cctttttctg tttttaggct gttttttcaa ccttcaagat ttttacatca tagctaccaa
      181 caggcgtttc aatggttgct gtgtcacctg ttttcttgcc aatcaaggcc tgcccaattg
      241 ggctttcatt tgaaacctta cctgcaaagg catccgcacc agctgaacct acgataatat
      301 aaacttcttc ttcgtcctca ccaatttctt ggatggtgac tgttttacca atcgctactt
      361 cgtcctgggc aactgcgtcg ctattgacga tttcagcata gcggattttt gtttctaagc
      421 tagagatttg tccttcgaca aaggcttgtt catccttagc tgcttcgtac tcactgtttt
      481 ctgaaagatc accgtatgaa cgggcaatct taatgcgttc taccacttct ggtcgacgaa
      541 ccaatttcaa ttcttctaat tctttttcaa gtttctcctt ttcctcaagg gtcataggat
      601 atgttttttc tgccattttt ctcaactttc ttctgataat attttctaaa gaaaattatg
      661 tgaagtatca cataatttta gtttgtttag tttaatttgc tgttgacatg ttcagcgaca
      721 ttgcggtcgt ggtcttcttg attgttagca tagtagacct tgccttctgt gacatctgct
      781 acaaagtata ggttatcgct cttagtttga ttgatgcttg actcaatcgc atccagactt
      841 ggactatcga ctggaccagg catgagacct acatttttat aaacattata aggtgaatca
      901 atgttggtat caatcgcaac atcctcagct agactgatat tttgccccag ttttccttgg
      961 gcatacaaga ttgcaatatt actttgaagt ggcatatcac gattcaaacg attgtagaat
     1021 acacctgcaa tgagcttacg atcttctgtc ttggcacctt ctttttcgac caaggaagca
     1081 atggtcaaca actcattgac agtcaagttt ttagatttga tagtactata gtaaggagat
     1141 aggttcttat ccatagcagc taacatctca tcaatcaagc tctcaatagt tgtgctttcc
     1201 ttgatagagt atgtagctgg gaaaaggtat ccttccaaac gataacgcgc accgctgtct
     1261 tttacaggca aactttccag taaagtagga tatttcgcta ctgcttgact gataaacgtc
     1321 tcatcttgaa ctttagccaa gaaagcctcc gctgtcaaag actctttgaa gtcaccttgc
     1381 aattgaccca cagtttgagc aatctgatcc aaggtataac cttctggaat tgtcaaagtc
     1441 gcaagtacag gttcttgcgg ttcatctgtt ccaccttttt gcaactcttt gagtaagtct
     1501 tctgtactca tactcttttg caaattgtag taacctgctt tcaagtcggt ataattttta
     1561 tacttggcat aaaaactaaa aatcagacca tgctttacca aaccagcttt ttcaagcgtc
     1621 gtaccgattt cttgaacgtt tgaaccttct ggaattccaa ccgtcacata tttcttagaa
     1681 ttagcatcga taggtaataa ggaatctagc acgtactggt aaccaaagta accacctgct
     1741 gagagcaagg caaggaatac aaggaatgaa atcacaaaag ctttcacatg tgacttcttc
     1801 tctttcttag gcttaacggg ttttgctcct tctctacggc tacgacgtga tgtatctctg
     1861 atgatatcca ctgtttctat agcaggcgta ggtgtttctg gacctgcttg ttctgctttt
     1921 ttctctgccg tcgtatagga aacagctacc cttgttgggg tttcattgta ttctctttca
     1981 agtttcttag gtctaacagg acctggacct ggtcttgatc cactttcttc cgctggagaa
     2041 gaaggtacat cttgacttgg atgacttgga acaccaggag tttctctttg aatctcatct
     2101 gctggagaag ctggtacacc ttgacttggg tgagtaggca cggtaggagc ttttctcata
     2161 atctcctcta ccgttgacaa ggaatcagcc atgagttctt cagctgaagg ttcatttgca
     2221 ggagtgcgaa ctactgcctc atcttctttc agaacttcat catagccttt tactttttct
     2281 aaatctctca gaatctgctc tttaaagctt aatttctctt cttctcttga cttttcactc
     2341 aaaagttttt cctccttgtt gataatccat aatattatat cttaaaagtc caaaaaaagc
     2401 aatctatgat acttttccta acttattttt tcatttccca gaccatatca taccatgttt
     2461 cccctgcaaa ggttgactgg gactggcctt cgttgacaaa tccatgtttt tcataatagg
     2521 cgatgagata gtcatgacag gttagattaa tgccatctct ctcatcttca agagcaactt
     2581 ctttcaaggc tgtcagcaat ttctgaccca gtccgagtcc ctgtgcctcc ttggcaatag
     2641 acagacaggt cacagagata taaccaccag gctcatgact atagtctttt atttcttctg
     2701 taaaagactg gtcttgcaga tggcggtgca ggccaactgg tccttcgata taacccatga
     2761 ttcttccttc tttttcagca accagaaaag aggtctgaat ttctctcaaa tgtgcttcaa
     2821 agacagaagg aggaatggct tcttcgaccg aaaaattttc aaattcaagt tcaacaatcc
     2881 gatccaaatc ttctaatctt gcttgtctga ttttcattgt tcctccagat aaaagggatt
     2941 aaaccaaatc atactatagc cctggctagt tgcatagagt aaagttgctt cttcatcaac
     3001 aaaaccgttc atttcaaaat aggaaagcag ctcatcagga ctctccaaac gaatcccttt
     3061 gtaatccagc tcaactgcca cctctttcaa ggctgcaaga agaagtgttc ccaggccctg
     3121 tctctgatgg tcagactcga tgactaaaga atgtactttt agacattgcg gattgtctga
     3181 ctggggactt gataaaatat agcctaaaag ttgattttca tccctagcta gaagaaaggt
     3241 atccgcacac ttacggatac tttcttctaa aatatgggaa agttgctgct tttcagctgg
     3301 aaaagacgag gtctgaagtg cccctatctc aggcaaatca gacttgcttg cctgaatgat
     3361 cttaattgga atttccatgg gaacatccta ttgaacattg cttgtcaagt tagacaagag
     3421 acgctcaaat gagtattcat aggtttggat gtctcctgct cccataaaga cgtaaacagc
     3481 attgtcatgg tctaggagtg gagaaacatt ttcaacagta atcacttggt gttttttgtt
     3541 gattttgttg gctaggtctt ctaccttaac gtcaccatga tctacttcac gagccgagcc
     3601 ataaatttgc gctagataaa tagcatctgc ttggtttaaa gcatgggcaa agtcgtccaa
     3661 caaggcaatg gttcttgtaa aggtatgcgg ttgaaagact gctacaattt ccttgcttgg
     3721 gtatttctga cgagccgcat ccaaggtcgc aataatttct gttggatgat gggcaaagtc
     3781 atcgataatc actgtatcat tgacaatttt ctcagtgaaa cgacgtttaa caccggaaaa
     3841 tgttttcaag tgctcacgca ccaagttcaa atcaaatcct gctgtgtaaa gaagaccaat
     3901 aacggctgtc gcattcatga tattgtgacg accaaaggtt ggaatgtgga attgccccaa
     3961 gttttgtcca cggaaatgaa cggtgaaggt tgaaccagtt gttgaacgaa gaagatcact
     4021 agctacaaag tcattgcctt cagcttcaaa accataataa taaattggtg catcagacgt
     4081 aatcttacgc aattcagcat cttcaccata gacaaaaaga cccttggtga tttgtttggc
     4141 atagtcgtta aaggcattga aaacatcctc gagacttgtg aaataatctg gatggtcaaa
     4201 gtcaatgttg gtgataatag agtattctgg gtggtaaggc atgaagtgac gctcatattc
     4261 gtcagattca aagacaaaat atttggcatt ggccgaacca cgacctgtcc catctccaat
     4321 caagaagctg gtatctgtaa tgtgagacaa gacatgagac aacatacctg tcgttgaagt
     4381 ttttccatgt gctcctgcta ctcccatgct aacaaagtca cgcataaagc tacctagaaa
     4441 ctcatggtaa cgtttgtagc tgataccatt ttggtccgca taggcaattt cgacgttgtt
     4501 atctggacga aaggcatttc cagcgataat ttccatatca ccgtctagat tcttttcatc
     4561 aaaaggaaga atggtaattc ctgcctgctc aagaccgcgt tgggtaaagt agtacttttc
     4621 aacatctgat ccctgaacct tgtgccccat ctggtgcaac atcaaggcca aggcactcat
     4681 ccctgatccc ttaattccga taaaatgata tgtctttgac atgttttctc ccctattctg
     4741 tcattctggt cagattcaac tcttgggcaa cccgacgttc ttgttctgtt tgtttacttt
     4801 ttttattgta gatttggctc ttctttagaa aatcataatt gtttttcttt ggagcaggtg
     4861 ctgacacttc ttcattcttg gtagggatag aatgaacttc ttccgccaag atataatgag
     4921 actgggtcaa tttttggcta tatttgacaa attcaccagg attttccttt tggaaaggag
     4981 ttgtcggttg attgccctgt ctaactagac tgggctgaga atgacgtctc gcaaggctga
     5041 aatcctgagt tagatagtta gcagagcgtt tctttttcaa gtctgcacgc gcttcttcac
     5101 gcgccacctc cgcatagctc tttccttctt ttttaacccc taaaggagct tttttaggtt
     5161 tccctacttg cttttcaatc ggttttactg gtttttcttc agcaatagga gcccattcta
     5221 aataattttt atctcgatac tcacccttga tattactgat cagatcagac tcatcataga
     5281 gattcatgac tggcatttca gtcaacatga cctcgtcatc tgacaccaat ggaaatcgtt
     5341 cttgtttcat tttctatttc ctttcaacac ttcattatag cgtattgtct tgatttttca
     5401 agtgctggct tcagaaattc ccaaaatttc tctaatttct gctagggtca gactaccacg
     5461 tgactctgtg ccgtccaata cttgtgacac cagatgtttc ttttgttctt ggagttcctg
     5521 aattttttct tcaatggttc ccttggtcac caagcgatag acctcaaccg tttcttcctg
     5581 acccatccga tgggcacggc caatggcttg cgcttccacc gcaggattcc accaaaggtc
     5641 aaccaagatc actgtatctg cacctgtcag gttcagaccg accccaccag ccttgaggga
     5701 aatcagaaag gcatctcttt ctccttgatt aaaggccttg gtcatgtctt gtctttcctt
     5761 ggctggggtt gaacccgtaa ttttaaagga agtcaggccc aagtctggca gttcttgttc
     5821 aattttttcc aacattccct tgaactgaga gaaaatcaag acacggtgtc cgccgtctgc
     5881 cacctgtacc agtaggtctc ggagactatc tagtttgccg ctggctccct gataatcttc
     5941 cataaacagg gcaggagtgt cacatatttg acgcaagcgc atcaaaccag ataaaatttc
     6001 cacacgactt cgctgaaatt cctgttctga cacttgagcc agatggtctc gcatctgttg
     6061 taactgggca aggtaaatag ccttttgctg gtcttccagt tcatttttat aaaccacctc
     6121 aatcaagtct ggcaattcag tcagaacttc ttctttcttg cgtcgcatca cgaaaggctt
     6181 gataaactga gccactcgct ctgctggcaa tttcataaat tctttcttgc ttggcaaaag
     6241 tccgggcatg acgatttgga aaatagacca caactcaccc agatggtttt caatcggagt
     6301 tcctgacaag gcaaagaccg acggcaccac aaattgtctc aaggtctggg caatcttggt
     6361 ctgggcattt ttcatgacct gagcttcatc taagaaaagg aagtcaaagg ccatcccctg
     6421 ataaagctca ctgtcctgac ggaaggtggc atagctagtc acatagattt gatggctctc
     6481 ggcaagaatc tcctcacgac ttgctttcaa accatgaaca acagccacat ccaactgtgg
     6541 agcaaatttc tgaaactcat ctgcccagtt gtaaatcaaa cccgacggag cgagaatcaa
     6601 aacccgactt tcttttgtca cttgactagt caaaaaagca atggtctgaa gggttttccc
     6661 aagtcccata tcatcagcca aaatcccacc aaaaccataa tgatggagca tctgcaacca
     6721 gccaattccc ttttcctgat aatctcgcaa gtcagccttg acctgagttg cttgcaaagg
     6781 aaagtcctct ggatgcgtca aatcctgggc cagattctgg aattcttgtg aaaaagaaac
     6841 acggtctcgc ccttcaaaga gatgagctaa actgtaggcc aaggatttcc gagcctgcaa
     6901 ggtcccatct tttaattcaa attgccccag ttcctgtaga ttttggcgaa ttttcttggt
     6961 ttcttcatcg aaaaagtaaa cttgattaga cgaatcaata taaaaatcct gattggcaac
     7021 caaggcctgc atggcttggt cgatttcctc ctgggcaata tcttgaaaat caaactggat
     7081 ttccaagaga cctcccttgg aagcaatctg cacctgagga ctcgctaggc tataaagctc
     7141 ttctagttta tctgataggt caacatgccc gagtttttca aagactggaa tgatatcatg
     7201 aaaaaaatga tagacagact ccgcttttaa ggcctgacgc caagattgaa aatcggattc
     7261 aaagcccgca gccaaacaga cttggaaaat tctttcttct aagtccgcgt cacttgaaaa
     7321 tggtaattct tctagctctt gtcggctaga tacctgtcta tttccataat caaactgaat
     7381 ttctaaacga atccgattat cttcttccct gtcaaagtaa aaagagggcg caaaagtttt
     7441 aatttgtaga cgttctggag ctgaaacggt gcccatctgg ataaaaagag tcagacagga
     7501 ggccaatttg tctcgatcac tgctatcaaa ttgcaggtat ttctttcctt gttgacccac
     7561 aggtaacgct ttaatttcct tgagaagacg catctgctgg tctgttaaaa aataaacctg
     7621 acctttatgg aaaagtactg ctccttgata aaagacattg accctaggac tctcactgat
     7681 ttccatttca aaatagtccg agtattctgt tactgtaaag gcaaatagat tggcatcagc
     7741 atgcatatcc tgaaaaagca gggtttggta gctatccact tgatggtcaa attgaaaatg
     7801 gggcaaggcc atcagtaaat tcacaccctg ctcaaaaaag gtcagaggga aaaagaggtg
     7861 ccgaccttgg ttttggaaaa agaggtctgg aaccagccct tcctccgtta gtccgtgcaa
     7921 gaaagtcaaa agttcttggc tggcatcatc aaaggcttcc caagaaagag actcctcata
     7981 aatcttgcca atcatatacg actttctctg ctcgacaatc cttaaaaaaa gtagaatatc
     8041 ccgaatgaca tagtattttt ggctattgat ttggccgatt ctcagagtcc acaagatatg
     8101 attggttcct gcttccacct gacccacagc tgataactca taggcgcatt ctgattttgg
     8161 agataaaatt cgattcaaaa acttgccacc caaggtcacc ttggtttcaa cggcctcttt
     8221 ttcttcatga ccttcttcca gactccacaa gatttcctga ccacgctcat catttttcag
     8281 aaaatgctct agcgctgcca aatgcacaca gtagcccctc ttttgaaaag aatcacaggc
     8341 acaaaaaacc aaatcatcct ctaaactata gcgcagttct tcttctgcaa cgcgagcgta
     8401 gagccgattg ttcttttcct tgatgatatc aaccttacca gtttcataaa gggcaacacc
     8461 ttcgatacga attttccccg gaatcaattt agccatattt tcacctttac cttatctttt
     8521 tattatacca tattttcgcc tatgaaaata gccttctagg aagacttttc tcctagaagg
     8581 ctggattttt aacgtttggc aaaagtagcc acaatccgct gacagacttc ttgcaacaga
     8641 gatttgggca tagctatatt gatgcgggca tggagacttc cttcctctcc aaaatccaaa
     8701 ccacggttga ggataacctt ggcttcattt ctcaacaact cttgcaatgt ttcatcagtc
     8761 aggtcataag ctgaaaagtc aagccaaatc aagtaggtac cttgcggttt catgaccttg
     8821 attttagtct cttttccaaa tagatccatc acataattga tgtggtcttc aaagacttgc
     8881 ttgagttcct ctagccaatc tttaccgtat cgataggcag cttctgtcgc caaataaccc
     8941 aagcctgaaa tttcatgctg attattggcc aacaggcgtt tctggaaagc cagtctcaac
     9001 ttaggatttt caatgactgc ataggaattt tttgttccag caatattaaa tgttttagtg
     9061 gcactgctca agacgatagc aaaatttttg aaggcaggat tgatggtatt gaaagactgg
     9121 tgtttgtgac caaagagggt caaatcttgg tgaatctcat ccgaaactaa caaaacaccg
     9181 tgtttttggc agagttggcc aatcttctcc aacacttctt tttcccaaac acgtccacca
     9241 ggattgtgag ggttgcaaag aacatagagt ttaacctcct cttccaccaa atccttttca
     9301 agttggtcaa agtcaatctc aaacagacta tccttttcca ctaaggaatt agtaatcaat
     9361 ctacgattat tcaacttgac actgcgagca aagggtgggt agacaggcgt gttaattaaa
     9421 accgcctcgc cttcttttgt aaaggtttga atagctgttg agatggctgg taccacaccc
     9481 tcgataaaga caagagcctc tttgtcaaag ttgtaaccgt attgtgtagc ttcccacttt
     9541 tgaacttcct taattaagtc ttcactggca taggtataac cataaaccag ttggtctgcg
     9601 taagtttgca cggcttggcg gatttcaggc aagaccacaa agtccatatc cgctatccaa
     9661 gctggtagaa cttcactatc cgtttctgtt tctttccatt tataggtatg gtgccctaaa
     9721 cggttgggca ggcttgtaaa atcatatttt cccatctttg tcttatcctt ctaaggcttg
     9781 gcgcaaatct gcaatcaaat ctctagtatc ctcaatccca atagacaaac gcaagaggtc
     9841 atctgtcaaa ccataagaat ggcgtacttc tgctggaata tcagcatgag tttgagtcgt
     9901 tggataagta ataagacttt ccactccgcc caaactttcc gcaaaagaga agaccttgag
     9961 actgttcaaa atatgaggaa tgcgtgtttc atcggctact ttaaaggaaa tcatgcctcc
    10021 acgaccagtg tagagaactt ccttaactgc tggagaatcc ttcaaaaagg caaccacttc
    10081 ttgggcgtta gctgttgagc gctccatacg aagagacaag gtcttgagac cacgaagcaa
    10141 ctggtagctg tcaaatggag acaagactgc ccctgttgta ttgagattgt aaaaaagctt
    10201 ctcgtatagt tctaaactat tggtcacaac cactccagcc aagacatcat tatggcctgc
    10261 tagatacttg gttgctgaat ggagaacgat atctgctcca tcttcaatcg gacgttggta
    10321 gatagggcta tagaaggtat tgtccaccac cactttggca cccttagcat gagccaattt
    10381 tgctagtttt tcgatatcaa attccaacat caagggattg gttggggttt cgatatagag
    10441 aacatccaca tccttttcta actcggcaat caactcttct tctgtattgg cataggtaaa
    10501 atggaaacga ccttcctgct ccacttggtt aaaccagcga aaagaaccac cgtaaagatc
    10561 acgcactgcc aagaccttac ttcctactgg aaagacgcta aaggccagta caatagctga
    10621 catccctgag ctagtcgcta gggcatagtc tgctgactca atagccgcca agacttcctc
    10681 agccttacta cgagttggat ttttagtgcg cgtatagtca aacccagtag atcgaccaaa
    10741 ctctggatgc tgataggtcg ttgaaaaatg aagtggtgtc accaaagcac ctgttgcctc
    10801 atcagacttg atccctgctt gtgctaaaat tgtgttaatg tgtaattcct tgctcataca
    10861 attcctccaa atctatagta actattgtac cacttatttt catcaactca ttttcttctt
    10921 ttcaagagct agttatagtt tcaaactata taaaaaggga gtttttcctg ctccctttaa
    10981 tagactataa aatggtgaat ctcaaaagac accttcactc tatcatttgc tcctgcacaa
    11041 aacgagcata acgctcatga ttttccagta gttccttatg agttcctgag ccagtgattt
    11101 tcccctcctc taagaagaaa atacaatcca catcttttac cgttgacaaa cgatgcgcta
    11161 taatcacaac cgtcttctcc tttagtacag aatagaggct actgataatc gcatactcag
    11221 aatccgcatc aagattagca gtggcttcat caaatataag aatttcagca tcttttaagt
    11281 aggctctagc tatttgaagt ctttggcttc gcccccctga caagagtcgt ccgcgttcac
    11341 caacttcagt atctagtccc tctttcatgg agcgaatctc atcacctagt gatactaagt
    11401 ctagcacttt catcaattca tcatcagtta ctaagcgatt caaaccgaga caaagattgt
    11461 cacgaatact gccagataag actgcattat tttgtgaaac ccaagcgatt ttacttctcc
    11521 attcttttaa gttaaaatca tatatacttg attgctccat tagaatatat cctgaaagcg
    11581 gtttataaaa ccgctctaac aaacgcacaa tcgttgattt tcctgatcca gatggtccaa
    11641 caaaagcaat tttttgcccc ttgaaaattg aacaagtaat atcctttaag acaggtcgat
    11701 tttcatcata accaaaatag acatggttaa aattcaaccc ttgtcctgat accgattttc
    11761 ctccctcaaa tttttcttta ggaactgcaa gcaagttctc cagtgcaact gaagatccct
    11821 tgctactaga ataaatagtt acaaaattag ctatattact aataggatta agtaattgaa
    11881 agaggtaaat caaaaacgaa accaaggttc ccacagatat atatcctgcg ctgacccgat
    11941 aacccccata ggttagcatc acaactatag tcgcaaagat aaataagaga gcaaacgagg
    12001 tctcaaaaga agtaacccta tctgatttca gtgaattgtt ttgtaccctt tcaatacaat
    12061 tatccaaaac atcctgtaca cttttctctg cttggttagt cttaattaat tcatgttctt
    12121 gaatcttttc agtcaattgc cctgttaaat ttcctcctgt aaacgacgac tatacttttc
    12181 actgatattg gaaaggggca agataataaa catcatacaa ggaagagtga tgaataaaag
    12241 tagagaaaga ttccaatcaa gactaaataa gactacaatg gaaccaagta ccataactaa
    12301 actcagaata atatttggga aagtcgtaat taaaaactca cgaatgacac tcgtgtcatt
    12361 gacaatggca gaagtcaact ccccactttg gctcttatca aagaaggatt tctctgcata
    12421 aatcaacccc tctatcactt ttttcctgat ttttgctatc tttttttcac ccgattgact
    12481 aaacagatag taaccaatag aagaaaacaa ggcttgacca ataaaaatca aaaacgattg
    12541 aaatactttg gaacctatat tttcaataga actcccatct attaaaccct ttaagataag
    12601 gggaagcaac aaagcaagta gactagacag aacaagtaag aaactcccca taatcac
//
LOCUS       DAVMEF010000049        12484 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMEF010000049 DAVMEF010000000
VERSION     DAVMEF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.049"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            67..231
                     /locus_tag="VYQ30_000983"
     CDS             67..231
                     /locus_tag="VYQ30_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1966122.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LVAVWLGWPK"
     gene            310..1038
                     /gene="yaaA"
                     /locus_tag="VYQ30_000984"
     CDS             310..1038
                     /gene="yaaA"
                     /locus_tag="VYQ30_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1966123.1"
                     /translation="MKILIPTAKEMNTDLPSIEATPLKPESQAVLDALALYSASQLES
                     FYKVSAEKATEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1316..1864)
                     /locus_tag="VYQ30_000985"
     CDS             complement(1316..1864)
                     /locus_tag="VYQ30_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1966124.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            2185..2673
                     /locus_tag="VYQ30_000986"
     CDS             2185..2673
                     /locus_tag="VYQ30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1966125.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            2670..3857
                     /locus_tag="VYQ30_000987"
     CDS             2670..3857
                     /locus_tag="VYQ30_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1966126.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVIDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            3850..4215
                     /locus_tag="VYQ30_000988"
     CDS             3850..4215
                     /locus_tag="VYQ30_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1966127.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            4231..5577
                     /gene="asnS"
                     /locus_tag="VYQ30_000989"
     CDS             4231..5577
                     /gene="asnS"
                     /locus_tag="VYQ30_000989"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1966128.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            5730..6020
                     /locus_tag="VYQ30_000990"
     CDS             5730..6020
                     /locus_tag="VYQ30_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1966129.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            6032..6502
                     /gene="ssbA"
                     /locus_tag="VYQ30_000991"
     CDS             6032..6502
                     /gene="ssbA"
                     /locus_tag="VYQ30_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1966130.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            6534..6773
                     /locus_tag="VYQ30_000992"
     CDS             6534..6773
                     /locus_tag="VYQ30_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1966131.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            7068..8468
                     /locus_tag="VYQ30_000993"
     CDS             7068..8468
                     /locus_tag="VYQ30_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1966132.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            8470..8829
                     /locus_tag="VYQ30_000994"
     CDS             8470..8829
                     /locus_tag="VYQ30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1966133.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            8883..9632
                     /locus_tag="VYQ30_000995"
     CDS             8883..9632
                     /locus_tag="VYQ30_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1966134.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            9622..9921
                     /locus_tag="VYQ30_000996"
     CDS             9622..9921
                     /locus_tag="VYQ30_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1966135.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            9992..10927
                     /locus_tag="VYQ30_000997"
     CDS             9992..10927
                     /locus_tag="VYQ30_000997"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1966136.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            11014..11526
                     /locus_tag="VYQ30_000998"
     CDS             11014..11526
                     /locus_tag="VYQ30_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1966137.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            11551..12159
                     /locus_tag="VYQ30_000999"
     CDS             11551..12159
                     /locus_tag="VYQ30_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1966138.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDIAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYVLKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            12260..12457
                     /locus_tag="VYQ30_001000"
     CDS             12260..12457
                     /locus_tag="VYQ30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1966139.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     ENAKGAVEGAVEGLKNVFTKE"
BASE COUNT         3762 a         2315 c         2838 g         3569 t
ORIGIN      
        1 aaataggttt ttttcagaaa gtatatgtta taataaggta aattaagcct aatggaggac
       61 aaagaaatgg gtttttattt gatggttgct tcaatgctac tcggactgct cgctttgaag
      121 ataggttttt ctcagttcaa ggagaaaaaa gataaattct tatctatctt aacaagttta
      181 gctggcatag cccttgttct cgtggctgtc tggttaggat ggcccaaata aatagaaaaa
      241 cctcggtcaa accgaggttt ttcaaatgaa tttcttggtt gtggctaaaa aatatgctac
      301 actatcaata tgaaaatttt aatcccaaca gcaaaagaga tgaacacaga cttgccaagt
      361 atcgaggcaa ctcctttaaa accagaaagt caggccgtgc ttgatgcctt ggctctctat
      421 tctgccagtc aattggagag tttctacaag gtatcagctg agaaagcgac ggaagaattt
      481 caaaatatcc aagctttgaa aaggcaaact gctcaacact atccagcctt gaaacttttt
      541 gatgggctta tgtaccgcaa cattaagaga gataagctga ccgaggcgga acaagattat
      601 cttgaaaatc atgtcttcat tacctcggct ttgtacggtg ttgttccagc cttgtcaccc
      661 atggctcctc accgtttgga ttttttgatg aaattaaaag tcgctggtaa gactttgaag
      721 agccattgga aggcagtcta tgatgaagct ctgaagaagg aagaagtgat tttctctctc
      781 ttgtcatcag agtttgagac tgtattttct aaggaaatca gagcaaagat ggtgaccttc
      841 aaattcatgg aggatagagg gggtcagctg aagattcact caactatctc caagaaggcg
      901 cgcggggcct ttctaacagc tttaatagaa aatcaagtac aaactgtggg ggaagcacgt
      961 cgcttgaact ttgctggatt tgtttaccga gaagatttgt cacaaccaca gggattggtt
     1021 tttgtaaagg aagtatagaa atcagatatt ttgtatttgg caatatgtat catcagctgt
     1081 tttagtttat aaatgagaaa aaaagcgaac aagctagtct tctgagtgtc agaaaagtag
     1141 tcttgttcgc ttttattatg ttatactctt cgaaaatctc ttcaagccac gtcagcgtcg
     1201 ccttaccgta tatatgtcac tgacttcgtc agttctatct acaacctcaa agcagtgttt
     1261 tgagcagctt gcggctagct tcctagtttg ctttttgatt tttattgagt gttagttact
     1321 tgatagcttc gaccaagtct tgagcttgtt tttcaagtga gtttaggact gtttcttcaa
     1381 gaaccaattt tccgtctgcc caggcagagt cattaacacg tgcagcagtg aaatcaccaa
     1441 cgccttgtgt acggataaat ggcaagaggt ctttgtagat agcgaaaagt tgatcgtgcc
     1501 ctgcattggc tacagatgat actgtgacaa acttgtcttg aagggcagaa acgccacgtg
     1561 tatcagacaa gtcaagggca cgagatagcc agtcaagcaa gtttttcact gtaccaggga
     1621 tagagaagtt gtagactgga gagaaaatcc agatagcatc cgcaacgaga actgcttcac
     1681 gagcagcagc tacagctgga tgtgttggaa cttccaaatc ttggctgaag agaggaaggg
     1741 ctgaataatc aaggtagcta acttccgctt taccagcaag tgctttctca gcttcgagcg
     1801 ccatttggtg gttgaaagaa ccttgacgta gtgatccgac gataaataat acttttttag
     1861 acatgatgtg tctccttttt cttaaaattt cgagaactct ctcgttgtta taaaatatat
     1921 tacaacaaaa caaaagaact tgcaagaaat ttgctcagag tttatttgaa aattgtaaaa
     1981 tttcggaaac tctaatcatt tgtgactgaa aaagtaagaa ctgtttcact ttcaaaatcg
     2041 gttttgtttg aagggagaaa tagaattggt tgctcaaagc acagctttga ggttgcagat
     2101 aaagctgatg tggtttgaag agattttcga agagtattct tatattcact tgtaataaag
     2161 cttaatagat ggtaaaatag acgagtgaaa aaaagacaaa acaaagcaaa aaataatcta
     2221 ctgtggcagt atggtctagg gatgacgatt ttgtttgtgg ttatcagtgc ttcctttctg
     2281 tatatggttt ctcttagcat gaaaccctat caaacagcta aaagtgaagg agaaaaatta
     2341 gctcagcagt atgcaggatt agagcaggtc gatcaggttg atttatacaa tggcttggaa
     2401 tcttattaca gcgttcttgg tcgtaataaa cagcaagaag cgcttgctgt cctgattggt
     2461 aaagatgatc ataagattta cgtttatcag ctaaatcagg gtgtttcaca agaaaaagca
     2521 gaaacggttt ctaaggaaaa gggagctggc gaaattgaca agattatctt tggtcgttat
     2581 caagataagc caatctggga agtcaagtca ggatctgatt tttatctagt agattttgaa
     2641 acaggagcat tggtcaacaa ggagggccta tgaaattatc caaccgtgtt ttagaaatgg
     2701 aagaaagtgt gactttggct gctggagccc gtgccaaagc actgaaggct gagggtagag
     2761 atattttgtc tctaaccttg ggtgagccag atttcactac tcccaaaaat atccaagatg
     2821 ccgccattgc atcgattcga gatgggcgtg cttcttttta tacagtaacc tcaggtctgc
     2881 cagagcttaa ggcggcggtc aatagctact ttgagcgctt ttacggctat tctgtagcgt
     2941 caaatcaagt gacagtcgct gcgggagcca aatattctct ctataccttc tttatggctg
     3001 tggtcaatcc aggtgatgaa gtcatcatcc caaccccata ctgggttagc tatggagatc
     3061 aggtcaagat ggcagaaggc gttcccgtct ttgtttctgc taaggaagac aatcacttta
     3121 aggtgaccgt agagcagtta gaagcagctc gcactgacaa gaccaaggtt ttggtgctga
     3181 attcgccatc taatcccaca ggtatgattt acacccgtga ggaactcttg gcaattggaa
     3241 actgggctgt agaaaatgat attctcatcc tagcagacga tatctatggc cgcttggttt
     3301 ataatggtca tgagttcaca ccgatttcta gcctatcgga agcgattcgc aagcaaacag
     3361 tggtcatcaa tggtgtgtct aaaacctatg ccatgactgg ttggcggatt ggttatgccg
     3421 ttggagaagc agacattatc gctgccatgt ccaagattgc aggtcaaaca acttcgaatc
     3481 cgtcagcagt agcccaatat gcagcagttg aggctctatc aggtgagcaa gatactgtag
     3541 aaagcatgcg tcaggccttt gaggaacgtc ttaatactat ctatcccctc cttgcagagg
     3601 tgccaggatt tgaagtggtc aaaccgcaag gggccttcta tctcttccca aatgtcaaaa
     3661 aggccatgga gatgaaaggc tacacggatg tgatagactt tacaactgct atcttagaag
     3721 aagccgaagt ggccttggta acaggagctg gatttggagc accagaaaat gtccgcctca
     3781 gctatgcgac agacctagac acgcttaaag aagcagtcga acgcttgaaa gcatttatgg
     3841 gtagtgagaa tgattgatca ttttgagatt aaggtaaagg atttacaaat ttcagaagga
     3901 ttttatagga gttttctcgc tcctttggac tataaaatgg cttttaagac tagttctcta
     3961 attagttttc tatccccgaa cagccctcat cctggtggtg atttttggct gactcaggga
     4021 acacaagatc ctgttcattt tgctttttta gcagaaaata aggaagaagt tcaggcttgt
     4081 tatgaggctg gcatagaggc aggtgggcga gacaatgggg ttcctggtta tcgaagtgag
     4141 catccgattt actatgctgc ttttatgatt gacctggatg ggaacaatat agaggtggtt
     4201 tgccataaag aataaaaaag aaaaggaaaa ataatgacaa aacgtgtaac gattattgac
     4261 gtaaaagact atgttggtca ggaagtgacg attggcgctt gggttgccaa caaatcagga
     4321 aaaggaaaaa tcgctttctt acaattgcgt gatggaacag ccttctttca aggtgtggct
     4381 tttaaaccaa actttgtcga aaaatttggt gaagaagtgg gacttgagaa gtttgatgtt
     4441 atcaaacgct tgagccaaga aacgtctgtt tatgtgacag gtattgtcaa agaggacgaa
     4501 cgttctaaat ttggctatga gttggacatc acagacatcg aagtgatcgg tgaatctcaa
     4561 gactacccaa tcacaccaaa agaacacgga acagactttt tgatggataa ccgtcacttg
     4621 tggctacgct ctcgtaagca agtagctgtg ttgcaaatcc gtaacgctat tatctatgca
     4681 acttatgagt tctttgacaa gaacggtttt atgaagtttg acagcccaat tctttcagga
     4741 aatgcggcag aagattctac agaactcttt gaaactgact acttcggaac gccagcctac
     4801 ttgagccaat caggtcagct ttacctagaa gcaggggcta tggctcttgg tcgtgtcttt
     4861 gactttggtc cagttttccg tgctgaaaaa tcaaaaacac gccgtcactt gactgagttc
     4921 tggatgatgg atgctgagta ctcatacttg acacatgatg agtcgcttga cttgcaagaa
     4981 gcttatgtga aagctcttct acaaggtgtt cttgaccgcg cgcctcaagc cttggaaacc
     5041 ttggaacgtg atacagaact cttgaaacgc tacattgcag agccattcaa acgtatcact
     5101 tacgatcaag ccattgacct cttgcaagag catgaaaatg atgaagatgc tgactacgag
     5161 catcttgagc atggtgatga ctttgggtca ccacacgaaa cttggatttc aaaccacttt
     5221 ggtgtgccaa catttgtcat gaactatcca gcagccatca aggccttcta catgaaacca
     5281 gttcctggaa atccagagcg cgtgctttgt gcagacttgc ttgctccaga aggctatgga
     5341 gaaattatcg gtgggtctat gcgtgaggaa gattacgatg cccttgtcgc taagatggat
     5401 gaacttggca tggatcgtac agaatatgaa ttctaccttg accttcgtaa atacggtaca
     5461 gttccacacg gaggatttgg tatcggtatc gaacgtatgg taaccttcgc agcaggaaca
     5521 aaacatatcc gtgaagctat tccattccca cgtatgttgc accgtatcaa accataagaa
     5581 caggaaaacg cccatgtggc gttttttcga attctagtgt ttttacttgc gtaaaaaaat
     5641 tttttctagt ataatagttt attgtgagcg aacctcactt accccttgca aagtcttggg
     5701 gtcattagac caaaaggagg aacatatcaa tggctaaata cgaaattctt tatatcattc
     5761 gtccaaacat tgaagaagaa gctaaaaacg ctttggtagc acgttttgac tctattttga
     5821 ctgacaacgg tgcaactgtt gttgaatcaa aaacttggga aaaacgtcgt cttgcatacg
     5881 aaatccaaga tttccgtgaa ggactttacc acatcgttaa cgttgaagca aatgacgatg
     5941 cagctcttaa agagtttgac cgtctttcaa aaatcaacgc tgacattctt cgtcacatga
     6001 tcgtcaaaat tgacgcgtaa gaaggtaaac tatgattaac aatgttgtac ttgtagggcg
     6061 tatgacacgt gacgctgagt tgcgttatac cccatcaaat gtagcagttg cgacttttac
     6121 tcttgcagta aaccgtacat ttaagagtca aaatggtgaa cgtgaggctg attttatcaa
     6181 tgtcgttatg tggcgccaac aggctgaaaa tcttgctaac tgggctaaaa aaggctcact
     6241 tatcggggtg acaggtcgta tccagactcg tagttacgat aaccagcaag gacaacgtgt
     6301 ctacgtgaca gaggtcgtgg ctgagaattt ccaaatgttg gaaagccgta gtgtgcgtga
     6361 gggtcacaca ggtggagctt actctgcacc aactgcaaac tattcagcac ctacaaattc
     6421 agtaccagac ttttcacgta atgaaaatcc atttggagca acaaacccat tggatatttc
     6481 agatgatgat ttaccattct aatggacaac taaacttata aaggagaaaa aacatggctc
     6541 aacaacgtcg tggcggattc aaacgccgta aaaaagttga ttacatcgca gcaaacaaaa
     6601 ttgaatatgt tgattacaaa gatactgagc ttcttagccg tttcgtttca gaacgtggga
     6661 aaatccttcc tcgtcgtgta acaggaactt cagctaaaaa ccaacgtaaa gtaacaacag
     6721 ctatcaaacg cgctcgcgta atggctttga tgcctttcgt aaacgaagat taaagaaaag
     6781 acccggttgg gtctcttttt catgagcttg aaaacaagaa agcgaattaa gaccccaatg
     6841 ggtctttttt cacaaacttt gaaatgagag agcgagttag gctcagtggc tcccttttta
     6901 cgagtttgaa aacaagagaa cacattaaaa gcacgatgga gctttttcat aaatatgtct
     6961 tttttctcat ccaagaaatt aaaataaaaa tgataaactt tttgaaaata ggggatttgc
     7021 ctactttatg atataatgga aagagttaga ttgaaagagg tagtgagatg gacgcgaaat
     7081 taagatacaa ggcaaagaag atcaagattg tcttttttga tattgatgat acattgcgga
     7141 attcaaagac agggtttatt ccaactacaa ttcccactgt ttttaaacag ttgcgtgaaa
     7201 aaggaatttt aacaggaatc gcctctggac gtggcatttt tggtgttgtt ccagagattc
     7261 gtgatctcaa gcctgacttt tttgtaactt tgaatggggc ttatatcgaa gataaaaaag
     7321 gtcaggtcat ttatcagcat cagattgaaa agtcagatgt tgaggagtat atctcttggg
     7381 ctaagcaaga aggaattgag tatggcttgg ttgggagtca tgatgccaag ttgtcgactc
     7441 gcaccgatat gatgagtgaa gctatcaatc caatttatcc cgacttagat gtagatcccg
     7501 atttccatga aaaagaagat atctatcaga tgtggacttt tgaagataag ggagatgact
     7561 tgcacttgcc tgatagtctc tcagacaaac ttcgcatggt tcgttggcat caacattcgt
     7621 ctgatattgt gccgatttca ggctccaaag cgacgggggt ggaaaaggtt gtggaacacc
     7681 ttggcttgaa accagagaag gtcatggttt ttggagatgg tctcaacgac ttggaactct
     7741 ttgattatgc tggaatcagc gttgctatgg gaatttctca tgataaaatc aaagaaaaag
     7801 cagattatat tacaaaaaca ttagaagaag atggcatttt tgatgcctta gaagtatttg
     7861 gtatggtaga aaaagaattg cattttccac aagtagacat tgaaacagta gaaggtcctc
     7921 ttgcgactat taagaccaat cacggagact tacgtatcaa gcttttccct gaacatgctc
     7981 ctaaaacagt ggctaacttt gtatctcttt caaaagatgg ctactatgat ggtgtcattt
     8041 tccaccgtat tatcaaggac tttatgatcc aaggtggaga cccaactgga actggtatgg
     8101 gtggcgagtc aatctacggc gaatcttttg aggatgaatt ctcagaagag ctttacaata
     8161 tccgtggtgc tctttccatg gcaaatgctg gtccaaatac caacggcagc cagttcttta
     8221 tcgtgcaaaa ccaacaccta ccttattcta agaaagaaat tactcgtggt ggttggccag
     8281 aaccgattgc agaaatctat gccaatcaag gtgggacacc tcacctagac cgccgtcaca
     8341 cggtttttgg tcagttagct gatgaagcat cttacgctgt cttggatgcc attgctgctg
     8401 ttgagacagg agctatggac aagccagttg aagatgttgt aattgaaact attgaaatcg
     8461 aggactaaga tgaaaatcgg tgataagcta aaggggcgta ttacagggat tcagccctac
     8521 ggtgcctttg ttgagttaga gacgggtgat acggggctga ttcacatttc agaaattcgg
     8581 acaggattta ttgaaaatat tcatgaaacc ttgaaagtcg atgaagaggt tcaagttcag
     8641 gtagtggatt tagatgaatt tacggggaaa gccagtcttt ctatccgcac tttggaggaa
     8701 gaaaagtacc agtttcctag acggagacgc ttttcaagcg accgctttaa ctacggtttt
     8761 gctccctttc gacgaatgct accaatctgg actggagaag ccctgcacca tttaaaaaag
     8821 aagaagtagt ttagagtttt atattttttg taatgttaat ataaatttgc tataatagga
     8881 ccatggaaga agaacaatta ttaaaatcag gagagcgcat taaccagctc ttttcgacag
     8941 atatcaaaat cattcaaaat agagaggttt ttagctattc ggtggatagt gttctcttat
     9001 cacgatttcc acgttttcct aagaaggggt tgattgtgga tttctgtgct gggaatggag
     9061 cagtggggct ttttgctagc actcgtactc aagcacagat attgtctgtt gagattcagg
     9121 agcgtttggc ggatatggct gaacgctctg tccgtttgaa tggattagag gagcaaatgc
     9181 aggtcatctg cgatgatttg aaaaatatgc ctgctcacat tcaggggagt aaggtggata
     9241 tgattttgtg taatccaccc tatttcaagg tggatcctta ttctaatctg aacgagagtg
     9301 aacattatct cttggctcga cacgaaatca cgactaattt ggaagaaatc tgtcgtagtg
     9361 cccagagtat tctcaagtct aatgggcgtt tggccatggt tcatcgtcct gatcgacttc
     9421 tggatatttt ggatacgtta aaacgacata atctagcccc taagcgcctg cagtttgttt
     9481 atccaaaaag agaaaaggaa gccaatatgc ttttgattga ggcgatcaag gatggctcaa
     9541 caagtggctt taaggtctta cctcctttga ttgtccacaa tgatgatggc tcttatacgc
     9601 ccgaaatcga agagatttat tatggatcat aaggcttata tgtacgttct ggagtgtcgt
     9661 gatggatcct actatatagg ctatacgact gatgtgagaa gacgcctcgc tatccacaat
     9721 agtgggaagg gagccaaata tacacgagca cgcttgccag tcaaacttat ctatgctcaa
     9781 ggttttgcca gtaaggaaga agccatgtcg gctgaagctc ttctcaagcg taagaagagg
     9841 ccacagaagg aagaattttt atctgaaaat caagatagaa atttactcag ttattttgaa
     9901 gaatcgtggg gagtcctttg actcctctta cctttgtcaa aaagcgaaaa aaatgctaca
     9961 ataaagaaaa taaacaaaat gaggtattat catgtccaag attctagtat ttggtcacca
    10021 aaatccagac tcagatgcca tcgggtcatc tgtagctttt gcctaccttg caaaagaagc
    10081 ttacggtttg gatacggaag ctgttgccct tggaactcca aatgaagaaa cagcctttgt
    10141 cttgaactat tttggtgtgg aagcaccacg tgttatcact tctgccaaag cagagggggc
    10201 agagcaagtt atcttgactg accacaatga attccaacaa tctgtatcag atatcgctga
    10261 agtagaagtt tatggtgttg tagaccacca ccgtgtggct aactttgaaa ctgcaagccc
    10321 actttacatg cgtttggagc cagttggatc agcgtcttca atcgtttacc gtatgttcaa
    10381 agaacatggt gtagctgtgc ctaaagagat tgcaggtttg atgctttcag gtttgatttc
    10441 agataccctt cttttgaaat caccaacaac acacccaaca gataaaatca ttgctcctga
    10501 attggctgaa ttggctggtg tgaacttgga agaatatggt ttagcaatgt tgaaagctgg
    10561 taccaacttg gctagcaaat ctgctgaaga attgattgac atcgatgcta agacttttga
    10621 actcaacgga aataatgtcc gtgttgccca agtgaacaca gttgacatcg ctgaagtttt
    10681 ggaacgccaa gcagaaattg aagctgcaat gcaagctgcc aacgaatcaa acggctactc
    10741 tgactttgtc ttgatgatta cagatatcgt caactcaaac tcagaaatct tggctcttgg
    10801 tgccaatatg gacaaggtcg aagcggcttt caacttcaaa cttgaaaaca atcatgcctt
    10861 ccttgctggt gccgtttcac gtaagaaaca agtggtacct caattaactg aaagctttaa
    10921 tgcgtaagat tttgggtgtc agctcaaaat cggaaagtct agtttgcctt atatcgaaag
    10981 gagtttcggc tccttttttt ctaggagtga agtatgttag aaaatggcga tttgattttt
    11041 gtgagagatg ggtcagacat gggacaggcc atccagactt ccacaggtaa ctatagccat
    11101 gttgccattt atttggatgg gatgatttat catgctagtg gacaggctgg tgttgtctgt
    11161 caagaaccgg cagacttctt tgagtccaat catttgtacg acctctatgt ttatccagaa
    11221 atggatatcc agtcggtgaa ggaaagagct tgcagacatc ttggagcacc ctacaatgct
    11281 tctttctatc cagatgcagc tggtttctac tgctcccagt atatagcaga aatcctacct
    11341 atttttgaaa ctattcctat gaaatttgga gatggagaac aggagattag tgatttttgg
    11401 agggagtatt acatagaact aggtctgcct gttcctctga accaagctgg gaccaatcct
    11461 agtcagttgg cagcatcgcc tctgttacaa tgtaaagaaa ggaatcttca tgattcagat
    11521 ttttaatcca tctcgtttga cgagacagcc atttttggag aattgatccg ctatctggac
    11581 cagtatgagg atgtgattct acgggaaatt aaggctcaat ttccagatat tgcagttgat
    11641 aaactcatgg aagagtatat aaaggcaggc ttgattctac gtgaaaataa gcgctattac
    11701 ctcaattttc ctacgcttga atcacttgat agtcttgaac tggatcaaga gatttttgtc
    11761 agagaagcta gtccggtcta tcaagccttg ttggagcaga gttttgagac ggaattgcgc
    11821 aatcaaatca atgcagctat tttagttgaa aagacggact ttgcgcgcat taaaatgacc
    11881 ctgtccaatt atttttacaa ggtcaaacag cagtatcctt tgacagaaaa acagcaggag
    11941 ctctatgaca ttttaggaga tgttaatcct gagtatgtcc tcaagtatat gacggctttt
    12001 ttgttgaaat ttctcaaaaa agaccagctt atgcagaaat gccgtgatat ctttgtggac
    12061 agtttggttg tcttaggcta tattgtgcaa aatgaagatg gaaagtatga gttggctatc
    12121 gattttgata aggagagatt aactttctac ttagcgtgat ttcttgtttc tgagcacatt
    12181 gtttgacttt ccttagtatt cggtataaac tatatgtaac cggtaacaca tatcggaata
    12241 aactaaagga tacaatcata tgtcacttga aaacaaattg gaacaagcaa caggcgctgt
    12301 caaagaaggt tttggtaaag ttactggaga cagcaagaca gaacttgaag gagctgttga
    12361 aaaaacagtt gctaaggcaa aagacgttgt agaaaacgca aaaggtgctg tagaaggtgc
    12421 cgttgaaggt ttgaaaaacg tttttactaa agaataggaa aaaatcaagg gtttcatttt
    12481 ccct
//
LOCUS       DAVMEF010000050        12390 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMEF010000050 DAVMEF010000000
VERSION     DAVMEF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.050"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(31..1914)
                     /gene="cbpE"
                     /locus_tag="VYQ30_001001"
                     /gene_synonym="pce"
     CDS             complement(31..1914)
                     /gene="cbpE"
                     /locus_tag="VYQ30_001001"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1966140.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDHVFRHLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYYKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKEKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(1917..2804)
                     /locus_tag="VYQ30_001002"
     CDS             complement(1917..2804)
                     /locus_tag="VYQ30_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1966141.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(2794..3255)
                     /locus_tag="VYQ30_001003"
     CDS             complement(2794..3255)
                     /locus_tag="VYQ30_001003"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1966142.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(3252..4160)
                     /locus_tag="VYQ30_001004"
     CDS             complement(3252..4160)
                     /locus_tag="VYQ30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1966143.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            4153..4350
                     /locus_tag="VYQ30_001005"
     CDS             4153..4350
                     /locus_tag="VYQ30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1966144.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSSQPY
                     FQNQFKKITGYTPLQYRKEKR"
     gene            complement(4402..5073)
                     /locus_tag="VYQ30_001006"
     CDS             complement(4402..5073)
                     /locus_tag="VYQ30_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966145.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(5127..5348)
                     /locus_tag="VYQ30_001007"
     CDS             complement(5127..5348)
                     /locus_tag="VYQ30_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966146.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            5620..6429
                     /locus_tag="VYQ30_001008"
     CDS             5620..6429
                     /locus_tag="VYQ30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1966147.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(6610..7485)
                     /gene="aguB"
                     /locus_tag="VYQ30_001009"
     CDS             complement(6610..7485)
                     /gene="aguB"
                     /locus_tag="VYQ30_001009"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1966148.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(7495..8580)
                     /gene="aguA"
                     /locus_tag="VYQ30_001010"
     CDS             complement(7495..8580)
                     /gene="aguA"
                     /locus_tag="VYQ30_001010"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1966149.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPK"
     gene            complement(8577..9704)
                     /gene="nspC"
                     /locus_tag="VYQ30_001011"
     CDS             complement(8577..9704)
                     /gene="nspC"
                     /locus_tag="VYQ30_001011"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1966150.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(9704..10963)
                     /locus_tag="VYQ30_001012"
     CDS             complement(9704..10963)
                     /locus_tag="VYQ30_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1966151.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(10960..11820)
                     /gene="speE"
                     /locus_tag="VYQ30_001013"
     CDS             complement(10960..11820)
                     /gene="speE"
                     /locus_tag="VYQ30_001013"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1966152.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            complement(11851..11988)
                     /locus_tag="VYQ30_001014"
     CDS             complement(11851..11988)
                     /locus_tag="VYQ30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966153.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            12075..>12390
                     /locus_tag="VYQ30_001015"
     CDS             12075..>12390
                     /locus_tag="VYQ30_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1966154.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAH"
BASE COUNT         3658 a         2783 c         2248 g         3701 t
ORIGIN      
        1 aaaaagaagg ccctaaaacc ttcttttcta ctactgttct gattccgatt tgtttacact
       61 cgtttcagag ctttgtgaag ttgaggatgt caaatcttga ctagttgaag aatttgtaac
      121 actttcatgt tgactcgttt ctttattttc aagatccttt ttcactactt ctttggattc
      181 ttttatttct gaatgacttg tagttttaat tgtagcagat ttctcgatcg gaacatccac
      241 taaccaggca ccacttgaat ccacagtata gccttctggt gtcttaccat tcacaagtaa
      301 ttggccattt tctttcaaga agtaccattt atccttctct ttaatccaac ctatagctcg
      361 tgaaccatct gtcttgtaga aataccaatc atttgccttt ttaagccagc cttgcttcat
      421 agctcctgat tcttctagat agtaatgatg tccagcaact tctatctcac ctgtcttcat
      481 gataccagta gaatccatat agtaccaggc ttctttatct tttacccagc ccgttttcat
      541 ttcccctgat ggagcaaagt aataccattg ccccttatat tgaagccaac ctgtcttcat
      601 agagccatca acatccaaat aataccactg ctctttatct tgaatccaac caatttccat
      661 ctggttttct ttgttaaaat aataccagat tccagcgatt ttcttccaat ttttagcagc
      721 aacaccagag tctgtcaaat agaaccaatg attgttccat tttttccatt gattctgtaa
      781 taagataccc gtttggttaa agtaatacca ttcaccctta atttcattcc aaccgacagc
      841 atattctcct gtagaatcag gcgcttgata ccaccagttt ccatatgaac tcttatgcca
      901 accagcttga aaacttggaa taggcttgta ggatgttgaa atattgacaa aaccgtcttt
      961 tcgaatatca aaaactgtcg catcatagtc tttgctggct gcgttgattc tctcaattcc
     1021 tcgttctttg agccaattaa catactcact atcaacacca tttttccaag gtagactatc
     1081 cgaagtttga acaatcaaac tcggactcaa atttttaatg aaatccttgg tatttgattt
     1141 attggtatca tggtgatgat taaacttcat caaatcaact tttccaatga gaggaccata
     1201 cttgtcttct gctccatgaa cattatctaa atcgccccca aggtaaattt tcttgccatt
     1261 gactttcacc acgctaatca aggaattgga attgtcatcc caaattttct ttaattcacc
     1321 cgatgaatca gtttcatttt cataattata gagctgaata tccatatccc caaactgaaa
     1381 atgagcatcc ccttgtgtga tattttgaat aactgaaaca cctttttctg cagcagtctg
     1441 taaaacctta taatagccat acagattatc ccatagacgt tcagaattag taatacgact
     1501 atcactatat ttcttaagat agactcggtc aactggatag gtagacagta attcatcaac
     1561 atttccaata tgatcactgt gggtatgggt caccaaaata aaatcaagtt tttggacacc
     1621 caattccttc aaatgacgaa agacatggtc tgtcagaaca tgcttataag acgtttcaat
     1681 tccttctctc catggatagc gagaatcact tccatctggg aaatcataat cttctcctgt
     1741 atccaccatg gcaaaatgtc cattgctttc aagaataatc gcatcactgc caccttcttg
     1801 aacattgata aagtggattt tatttcctga actttcttga gcctgaacat tcccatacca
     1861 tgacaaacct aaaaaagcgc ctacaagtgc taaactagtt aatttctttt tcattcttac
     1921 tttctcaatc tctccaaggt ttccttaaaa atctctggga tatctgcttt aaattccaag
     1981 gtcttacctg ttctcggatg agtaaaacct aaagtcttgg catgaagaaa ttgtccatgt
     2041 cctttcaaag tctttcgagg accatagacc tcatcaccag cgactggatg gccgatataa
     2101 gccatgtgga cacggatttg atgagtgcgc cctgtctcca gttgcaactc tactaagcta
     2161 taatcgccaa agcgttccaa gacgtgaaaa cgcgtcactg caggcttccc tttagcagtt
     2221 acagcctgtt tcttacggtc tttttcactc cggccaatcg gcgcttcaat tacaccacga
     2281 tcattaggca gatttccatg aacaatcgcc caatatttgc ggagagactt tttatccttg
     2341 agttcttggg caagtgctag atgcgcatca tcgtttttag caatcatgag aagacctgac
     2401 gtatccttat caatacggtg aacaatccct ggacgcagaa ccccattgat acccgacaag
     2461 tccttaatat gatacatgag ggcatttact agggttccac tggtatgacc agcactcggg
     2521 tgcacaacca ttccctgagg tttgttaacg acagccacat cctcatcttg gtagactatt
     2581 tctagcggaa gatcctcagc cacatactct aatacctctg gttctggcac atggtaagtg
     2641 acgacatcac cctcttggac tgtgtattta gctttcttga cttgaccatt gaccaagacc
     2701 tggcctgatt taatttgttc attcgcgaga ctacgtgata attctgacaa atctgacaaa
     2761 gccttatcca aacgcagacc accagtttca attttaattt ccatttattt cctcttttag
     2821 cattgcaatc aataaaataa tcactccaac cgtcagatag ctatctgcca cattgaaaat
     2881 tgcaaagttg ataaagtcaa ggtggaacat atccacaaca aagccctgac tgaccctgtc
     2941 aataaagttt ccaagaccac ccgcgattat tagagtcaaa cccaagacca tccagaatga
     3001 gtcctccatg tgtttatgta aataccaaat ggcacctatc acgacaacca gagtaatgac
     3061 agcgaataac agctgctgat cttgtaggat agaaaaggct gcacctcgat tttgcaggta
     3121 ggtcaagcta acgaaattgg gaatccagga gcgcacttca cccagtggaa tctgctggac
     3181 gatataggat ttgaccaact gatccagccc aattaaaagc agtacaatga ctgccactat
     3241 tgctcttttt ttcatgattt cctcttttga tcaaaatatt cttgcatgac ttctacgaag
     3301 agagtcccag cttgactaag ctccacttct tcacgtttaa catagaccat gcggttatct
     3361 aggttatcct tgagacgaat aactgtaatg ccattaacac tgtcactatc taaaaatcca
     3421 gaacctgtcg cataggcgtc cgtccgctcc aaaataccat tcaaggtggc acggtctgtc
     3481 acattaaaca tctgtgagct agcgctggta tcgacaaagt tctctgaata ataaaggtac
     3541 tcgtctttct cttgagtgaa acgaaccgtt ggtaaatccg ctaaatcctc catgactaat
     3601 tcctctttct gggctaaagg atgaccctca cagagataaa tatgggtatg gaaaggaatc
     3661 aattcgatga cctccagacc taacttttca acccgttgca taatcccctt tttattttga
     3721 ttgttgaggt agataatccc aatctcacta tgcccttgcg ccacttcatc taatatttga
     3781 acagtagttg attcaaaaat acggaagttc ttatagtcag gatagcgctc tgaaaaggcc
     3841 gtaatagttg gtggtaagaa gtcatagtgc tggctagcaa cggaaaattc atctttttct
     3901 tcttcaggat tggcatactg attttgaaaa atatcaaatc ctttaaccaa ttcttgcgct
     3961 ttttcataaa attccatccc acgacgggtc aagaaagtcc ctgagctggt ccgacggaaa
     4021 atcttaaagc ccaactcttt ttccaaatca cgaacagaaa tagacagact cggctgacta
     4081 acatacatct tttcagcagc ttcacgaaaa gtaccactat tggcaatagc cacaacatag
     4141 cgtaattgtt gaatgttcat cttctacccc caacttcttt atcttttcat tataccatat
     4201 tttagaagtt ttccaaaaag gaaaaaagaa catcctattc ttcttaacta tcttcactat
     4261 ctgcctttct caagccaacc ttattttcaa aatcaattca aaaaaataac tggctacaca
     4321 ccactacaat atcggaaaga aaaacgttga ctatttgtga aaaaagaaaa tgccggaaaa
     4381 ttccgacatt tttttagtaa gctaacttcc tgaaaatagg agtaaccaaa catcccagaa
     4441 ggcagtgata ttgatgagaa tatgaactag tattggatag tagagatttt tagtcatgcg
     4501 atacaataga gctaaaatca gacctccact agcataaatg aaaaaagcaa gaggagttaa
     4561 agcaaaattg atatgaatat aaccgaaaat aatagcagaa agcaaaacat ctccgtacca
     4621 aggtgagttt ttgaaaaagg ttgtcataag cacacctcga taaatcaatt cctcagcaat
     4681 aggggcgatg aagcaaacga tgagcaagaa ataagggaac tcctgtctcc ccatcatttc
     4741 tatcgtttca ttcaaagaaa tctgatttga agacagggat atgaaatacg aaaagaggaa
     4801 gtcagacata tatgaaatga tgtagcccag taaaaggtag atgaagtacc tcagctgcca
     4861 cttgaaatga aaaattttct ttcctgcaaa aaccagtaga tagatgaccg ctaagaaaag
     4921 aactccgctc tccatcaaaa gcagaatctg aaaaaattca cgacttgctg gtagataggg
     4981 ctttgcgagg tgattgaaaa ctctccaaaa ccaagttgat ttgtaaaaaa ttaaggacaa
     5041 tgctagtaaa atttgaatag cccgtttttt catattaaat tctctgcttt ctccccttgt
     5101 ttcttcatat tcctaataaa ttttacttaa aatctcgcag cacttccttt gcaaagatga
     5161 ttacctcctc caatatatcc taaatcatcg gttcctctgg gacaaaatcc atgaaccggc
     5221 atagttaata ctggttccat ttgccaagga taataaccat taggttcttt accataggga
     5281 tctgctccac cttgtatcag ctctaattct tcatttgtta gatttatatt ttttgtcatt
     5341 aaaatcatga tatatttcct ccgttattgt ttagattagt tgagtaatct gatactcata
     5401 cctacttaca aaaaaactat tatattaagt tggtttttta atttgtctag ttgcaacatt
     5461 gacaaacata gtatagcata tctttgccaa atatcctctt caaatcatga attgtcatca
     5521 aaacatctta aactataaaa tcaattagtc tcaagctttc tatcaatttc ttctccaaaa
     5581 tatgctataa taataccaaa agataaagaa ggaagaccta tgattaaact actagccttg
     5641 gatatggatg gaaccctcct caatgaagcc aaggaaatcc cacaggctca catcgctgct
     5701 attcaccaag ccattgaaaa aggtgtcaaa ctggttctct gtacgggtcg cccccttttc
     5761 ggtgtcctcc cctactacaa aaaactggga ctcgacctcc agaatgagta tgtgattgtt
     5821 aacaacggtt gttcaactca ccagactagc gactggggct tggttgactg gcaagaactt
     5881 agtccagctg acatcgaata cctctatgac cttgctgaaa agagtgatgt tcagttgaca
     5941 ctttttgacg agtcacatta ttttgttctc ggtggcaagc ccaatcaagt tattgaaaat
     6001 gatgctaaac tagtattttc agacctgact gaaatttctc ttgaagaagc gactagtgga
     6061 aagttccgga tgttccaagg tatgttttta ggaacaaaag aacaaacaga cgattttgag
     6121 cagcgttttg ctgaagagct ttgccaacga ttcagtggag ttcgttcgca gcctgtcatt
     6181 tatgaagcaa tgccgcttgg aacgacaaag gctactgctc tttcacgact agctgagatt
     6241 ttgaagattg attcctcaga gattatggcc atgggcgatg ctaataacga tatcgaaatg
     6301 ctccagtttg cagggcttgg gattgcaatg ggaaatgcca gcgattatgt caaatctctt
     6361 gcggatgccg ttacctcaag caacgaagaa gacggcgttg cgcgtgctat tgagaaatat
     6421 attctataaa aaagaaaagc aaatagacag aagttactgc tatttgcttt tttgctaata
     6481 ttttaaaata cggactaatc aatagacaag aatagcgaat cataaaaatc gatttactag
     6541 atgccatagt atcttatagt tgctaataag aagttagtct agcagaatga atctctcatt
     6601 tctcccacac taatctgtta tttgtctata catttctggt cttctatctc gaaacaagcc
     6661 ccagtttaga cgttcacttg ctcccttgtc taggtcataa gtagctaaca gaacagcttc
     6721 ttcttgtctt tcagctcgtt ctagaatagc tcctgtttca tccgtcataa aggaggaacc
     6781 gtagaagtca agactggaac tctgtccgcc attttcctca ctaggagtaa cctcctctaa
     6841 accataacga ttggctgcga tgactggaac aatattcgct gctgcgtgcc cttgcatagt
     6901 acgttgccag tgaccacaac tatctgtatc caaaatcggc tctgaaccga tggctgttgg
     6961 ataaaagagc aattcagcac cattcaatgc aagacagcgc gctgtttcag ggaaccattg
     7021 atcccaacag ataccgatac caatcttagc atagcgagta ttccagacct tgaaaccagt
     7081 gttaccaggc gtgaaataga atttttcttg ataataatgg tcatctggta tgtgggtctt
     7141 tcgataaacg cccagcactt ccccatctgc atcaatgacg gcaatagagt tatacaagac
     7201 attaccatct ttttcataga aactgattgg taaaacaact tgtagttcct tagcaatcac
     7261 cttaaaatgc tgaatggcag tattttccgc tacagattgg gcatactggt agtagtcata
     7321 ctgacgttcc tgacagaaat agggatgttc aaacaactcg ggcaagagaa taatttgggc
     7381 tccttgctca gcagcctgac gtactaaacg ctctgcggtt tggatatttg ttgccacatc
     7441 cttagcgcat tgcatctgaa tggttgcaac tcttacattt ctcatctttt tctcctattt
     7501 tggaatttgt tgggtgatac agtggatatt gccaccacct aagagaatat ctctggctgg
     7561 tattccgaca actttacggt ctgggaaaca cttgctgagg atatctaagg ccacttggtc
     7621 gtttacatcc tcaaactgtg gaaccaagac agccttgttg gcgatataaa agtttacgta
     7681 ggaagctgct agtcgttcac ctgcgtatcg ctcttcttct ccttcttcat agatgtagcc
     7741 tggcaaatct tcttctgtca caacttgtcg aactgcaggg ataggcaatt tatgaatggt
     7801 gaagtgacaa ccttttgcat ctgtttcctg ttctaagagt tcgagatctg cttttgacat
     7861 ggcatactgg ggatcatttt cgtcatctgt ccaagccaaa acaacctcag caggaccaac
     7921 aaaggcagca acattatcga cgtgttcatt ggtttcatcc tgataaatac cataaggaag
     7981 ccaaataact ttttcagcac caagactttc taataatgtg ttttcaatct cctctttagt
     8041 caggttagga ttgcgaccag gactaagcaa gcaactttca gttacgagaa tagttccttg
     8101 accatcgcta tggattgcgc ctccttccag tacaaaaggt ttagcatcat agacaggcct
     8161 ttccaaggcc tcagcaaaac gactggctac ttggtcatcc tcttcataat cttgataaag
     8221 accatcatag gtgcctcccc aagcattaaa ggcccaatcc acggctaatt tcttaccttt
     8281 atcattgacg agaatggttg ggccagtatc acgcgcccag gcatcattgg tgggaatgtc
     8341 taaataaaca accttgtctc caagatagga ttgggcttca gatagatagg cctgctccac
     8401 caaaagatag actctttccc cttctgctat ggtctcgatg atctgagtaa atgctctttt
     8461 agcagccttt ccttgaaaag gccatgatcc tggtcgagtc ggccatatca tgagggtacc
     8521 atgatggggt tcgtactctg ctggcatgtg atagcctaat ttttttggac tgtccatcat
     8581 gataatctcc ctttaaagtc ttgatagcca aaagctttga gtaagctaca gtctccctgt
     8641 tcgtccatga gatagagact tggcaatcca ataccattaa aggtattatt tttgacaaaa
     8701 gaataaatgg ccatgtcttg aaaataaagt ctgtctccga tttggactgg attttcaaaa
     8761 ctataatcac caatcacatc gcccgtcaga caggtattag aagaaagtct gtaggtatgg
     8821 gctttttcct gtgcctcaaa gccatttctc aaaggtggac gatagggcat ctcaagtaca
     8881 tcaggcatat ggcaggtcgc agaggcgtct aaaaccaaga tttccatacc gttttctaca
     8941 atatctaata cctcagttgc taaataaccc gcattaagcg caatggcttc accaggctcg
     9001 atatagattt caagattgta agtttttcgg atacgcttga tttctgaaat cagcaaatcc
     9061 acatcgtaac cttctcttgt aatatgatga ccaccaccca tattgagcca ttttacctca
     9121 tgtaagtagg gaccaaactg ttcttctact gctttcaaag ttgtttgtaa atcatctgct
     9181 ccctgctcgc aaagggtatg aaaatgaagt ccgtcaacca aatctagcaa atcactcgga
     9241 atcttgtcta tagtaactcc aaagcgagaa cctggtgcac aagggtcata gagcgcgtga
     9301 tctccttgag ttgaacactg agggttgagg cgcaaaccaa cactgacacc agcctctcga
     9361 caacgcgaac catgcttacg caactgtctc tctgagttaa agacgatatg gtctgttatc
     9421 ccaagcaatt cctccatgtc ctcatccttg aaagccggtg caaaaacatg gacttcacca
     9481 ggaaattctt cccttgccaa tttggcctca tagagtccac tagctgtcgt acctgataga
     9541 tactggctaa tcaagggata agttttgtag agggaatatg ccttctgggc aagcaagacc
     9601 ttgcaaccgg cctcttcttg tacatattgt agaatgcggc aattagcttc taacttggcc
     9661 aagtcaataa cataggctgg tgttggtact tgttctaact tcattagtcc accatttgtg
     9721 gattttcaac cacaacccat ggcaaaccat actcattcaa agcttccatg aatggatctg
     9781 ggtctaactc ctcaaggtta tacactccag cttgtttcca agttccgttc atgactaatt
     9841 ttgtcccaat catggctgga actcctgtcg tataagaaat agcttgcgaa ccaacctctg
     9901 catagcactc ttgatggtcg cagacattgt agatatagat agtctttttg acaccgtctt
     9961 tgacacctgt aaagatacat ccaatattgg ttttacctac agtacgtggg ccaaggctag
    10021 caggatctgg aagcaaggct ttcaaaaatt gaattggaac aatttcttgg ccgttaaagt
    10081 taatggtatc cgtacgaagg agtccaacat tttcaagaca tttcatgtgc gtcaagtaag
    10141 attgaccaaa agtcataaag aaacgaatgc gtttgacacc tggaatgttc ttggccaatg
    10201 attcgatttc ttcatggtga aggagataca tatctttttg tccaacttga gggaaatcat
    10261 actcacgctt gatagacata gcttcgactt cgacccattt cccatcttcc cagtaagaac
    10321 ctggcgcaga aacctcacgg agattaattt ctggattaaa gttggttgca aatggataac
    10381 cgtggtcacc gccattacag tctaaaatgt cgatataatg gatttcatca aaatagtgtt
    10441 tgagggcata agctgaaaag acactagtta cacctgggtc aaaaccagaa ccaagaagag
    10501 cagtcaagcc tgcttctttg aatttctctt gataagccca ctgccatgag tagtcaaagt
    10561 aggctgtaaa accaagttcc ttacaacgtt tttcgtagat agcacgccac tcagggtctt
    10621 ctgtgtcttc tgcttcgtag ttggctgtat cgatatagtg aacacctgtt gccaaacaag
    10681 catccataat ggttaaatct tgataaggca gagctacatt caaaacagct tctggtttgt
    10741 agctttcaat caaggtaatg acctcttcta ccttgtcagc atcaagtgct gcagtttcaa
    10801 ttttagtact tgttttgcct tctagcttcg ctttcaagtc atcgcatttt gacttggtac
    10861 ggctagcaat cataatctct gtaaatgttt cgctatcttg acaaattttt gaaatagcaa
    10921 cttgggcaac gcccccacaa ccaataacta gtaaacgact catttttttc cttcctcttc
    10981 ttctaaaatg tcctcaacat acttgggcaa cataaaggct cccacgtgta agtttgcagt
    11041 gtagtattct gtgaaaagct ggcgtttttt ccaaccttcc ttgtcaaaat ctttgacagg
    11101 gtggtatttt ttcgatgcaa atccaaacaa ccaatagcca gctgggctag ttggaatatg
    11161 ggcctgataa acccgactga ttggaaaggc ttgattgacc ttgcggtgca tgcttcggca
    11221 ggccgactca tcctcgtcaa agaagggact cccatgctgg taaatcatga tgccgtcttc
    11281 cttcagagct cgataactat tgccgtagaa ttccttggta aagagtcctt ccgtatggcc
    11341 aaatggatct gtcgcatcgt tgatgataat atcgtaatca tcttcgcagt ttcgcaaaaa
    11401 gcgtagccca ttttggtagt aaatggtaac acgaggatca tctagccctg cagcaaagtc
    11461 tgggaaatac tcacgacaga cctcgaccaa catttcatcc ggttccacga tatcgatttg
    11521 ttccagttca ggatagaggg ttaatacttg ggcaacaccg ccgtcaccac ccccaataac
    11581 caatactttc tttggatttg ggtggacagc catgggaacg tggacggtca tttcattgta
    11641 gacgaaatca tccgcatctg agaacaagac atggccattt aaaatcagta ttttcccaaa
    11701 agctggcgta tccaagactt cgatatcctg ccattcactt tttccagcgt aaagttgctt
    11761 ggctgttctc agagacaatt tgacatctgg agtatgaact tcagaaaacc ataaatccat
    11821 ctatttctcc ttatattata tttagcgcag ttagctactg ccaaagccca agtggtatac
    11881 ttggaataag ccactgtgga ttagttcatt tcctccaatc gcttgacgat ttccgaaagg
    11941 ctctaaccac agcttttgtt tcaattggta atgagtttga taacgcatag acgttttatc
    12001 tcgtctaagg taacaaggca aaaagttcgt ggataaccaa gtagacttga aatactatat
    12061 ctggaggtac tcccatgctt tatgtgggca ttgatatcgc taaaaataaa cacgatgtta
    12121 cagccttgaa tgttccagga aaaactgttc ttaaaccact cactttttca aataataaag
    12181 ctggttttga actcttagat ctgtctcttc gacagctcaa ccaagactgt ctcatcgctc
    12241 ttgaagatac tggacattat gcctttaatc tactgaactt cctgcatgaa caaggatata
    12301 aagtttacac ctacactcct ttgctcatca aggaatttgc caaatcatta tcacttcgca
    12361 agactaaaac ggataagaaa gacgctcatg
//
LOCUS       DAVMEF010000051        12101 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMEF010000051 DAVMEF010000000
VERSION     DAVMEF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.051"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(672..1898)
                     /locus_tag="VYQ30_001016"
     CDS             complement(672..1898)
                     /locus_tag="VYQ30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1966155.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWRANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1941..2633)
                     /locus_tag="VYQ30_001017"
     CDS             complement(1941..2633)
                     /locus_tag="VYQ30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1966156.1"
                     /translation="MLKWEDLPVEMKSSEVESYYHLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2806..4557)
                     /locus_tag="VYQ30_001018"
     CDS             complement(2806..4557)
                     /locus_tag="VYQ30_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966157.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDDQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLTFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4547..6292)
                     /locus_tag="VYQ30_001019"
     CDS             complement(4547..6292)
                     /locus_tag="VYQ30_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966158.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYTIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAAVEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMAGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6498..6875)
                     /locus_tag="VYQ30_001020"
     CDS             complement(6498..6875)
                     /locus_tag="VYQ30_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1966159.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVALKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(6947..7403)
                     /locus_tag="VYQ30_001021"
                     /pseudo
     CDS             complement(6947..7403)
                     /locus_tag="VYQ30_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7537..8364
                     /gene="xth"
                     /locus_tag="VYQ30_001022"
     CDS             7537..8364
                     /gene="xth"
                     /locus_tag="VYQ30_001022"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1966160.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8376..8519
                     /locus_tag="VYQ30_001023"
     CDS             8376..8519
                     /locus_tag="VYQ30_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1966161.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            8519..8758
                     /locus_tag="VYQ30_001024"
     CDS             8519..8758
                     /locus_tag="VYQ30_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1966162.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9141..9236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9636..10217
                     /locus_tag="VYQ30_001025"
     CDS             9636..10217
                     /locus_tag="VYQ30_001025"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1966163.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIKIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10217..11479
                     /locus_tag="VYQ30_001026"
     CDS             10217..11479
                     /locus_tag="VYQ30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1966164.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPLDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11566..>12101)
                     /locus_tag="VYQ30_001027"
     CDS             complement(11566..>12101)
                     /locus_tag="VYQ30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV1966165.1"
                     /translation="KSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIV
                     QSYLEAEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLI
                     EVNFYSGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILP
                     FLYNINDLEHNILKNLK"
BASE COUNT         3587 a         2643 c         2416 g         3455 t
ORIGIN      
        1 taccttgtca tacaagcatt cgccagggat atttacctta atatcgctca tatggcggat
       61 tgaaactaaa acactacaat atatttctta aatagacctt ctataaaagc tagatttaac
      121 ctcgataaaa atagatgcag tgcttctaaa atactggaat agggcatata ggtaatcgta
      181 gacaagtgtt ttatagattg tctcgcagga gatctaatga tgtatagcaa gcatcactaa
      241 acatatggga tattcgtatt attataatat caacatgcta taaattctat catgtaaaag
      301 aatgactaga acatgaaaga caggcacaaa actttgaaca atattctgaa aacaaaaaaa
      361 agacgcgagt ttccacaatc tcactcatgg aaactcacgt ctttcaaaaa atagggtgaa
      421 ttgaaaacta gaacaagaca ctagattttc taaatgatgt ctagtaagga ttctaaatgc
      481 tacaccaaat tgctcacatc ttatttcaaa ccacgggact cccacaagaa aggaaaatcc
      541 tataaaaatt ctctctctga ggagaaagga gcctcaacta caggtgtaaa ctattatcaa
      601 ttgaaaacaa cagtttttac actcaattag actttttagg gacaggatca ctttcgcttg
      661 actgtagttt gtcatttttt tgataaagtt agcacttttt cagaaactgt tttgaaagtc
      721 tcaatgatat agtctacttc ttcatcgctt aatttagtat gaagagggag ggtaatttca
      781 ttctcaaaga aggcataggc cttaggatag ttcgtcatat caaatccaag attcttatag
      841 gctgtcaaga gaggaagcgg tttgtagtga acattacttg caattcctgc tttagccaat
      901 tcttggatga tgaggttgcg ttcttctagg cttgctcctt ctacacgggt gatgtagagg
      961 tggcgtgaag attcgacagt ttcagtcttg tgtgccaaag gatggatgcg agaacctgca
     1021 aaaccactat catagcggtc cacaatgtcc ttacggcgtt gcaacaaact tggatagcgg
     1081 tccaattgta ccaaaccaag tgaagccatg atatcggtca tgttgcactt ataggctggt
     1141 gtaacgatat cgtattccca tgaccccagt tgcatcttgg caagagcatc cttagtttgc
     1201 ccgtgaaggg aaaggatttg gaattccttg tacatctctt cgtcatcaat cactggattg
     1261 gctctccaag tcgcacttcc accttctgcc gttgtaaagt tcttaacggc atggaatgag
     1321 aaggaagtaa agtcagcgat agaaccagaa ggttgtcctt tataagtaga tcccaaagcg
     1381 tgggcactat cagagacaat gacaatacgg ttaaaggcct tttgccactt gcttgaagca
     1441 gtaaagaagt cacgtttttt ctccacgact tggaacaaac ggtcataatc gcaaacaatc
     1501 cctgcgagct ctactgggat aatcacctta gttttctcag tgatagcttg ctcaagcagg
     1561 tcatagtcca tctcaaacgt atctgcttgg atatccacca tgacaggggt tgctcccacg
     1621 tgcgtaatga cactacatga agccgtatag gtcatggctg gaacgatgac ttcatcacca
     1681 ggtcccactt ccaaaacgcg taaaatcaac tccagagcgg ctgtcgcaga gttgagacaa
     1741 acagtcttag gtgtttgtgt gtaaagagac aagcggcgct ccagttcttt tgttttagga
     1801 cctgttgtga tccaaccaga acgcagggta tccgctactt cagcaatttc tgcttctgtg
     1861 atatcaggcg gtgaaaatgg aatattgtaa tttggcattg atttgctcct tttatctgta
     1921 ctcattttct tatgactact ttattttagt acctcaaaca cggtttgaaa catgattttg
     1981 atgtccccaa agaaactaaa ctctcggaga taggcgaggt tatagcgcat cttttcagga
     2041 agaacatgct ccacataggc ctgatcaact gacagacctt tctccgtcat ttgactgatg
     2101 atggtgtcct catccttgta gttgatgctg gctggagagg taattcctgc ttgcaagagc
     2161 aaggttgcca tcatttcagg gctatactgc tctgtataac gtggcacttc aggtcgtgta
     2221 ccgacaaagg acatctcacc tttaaggaca ttgaccaact gaggcagttc gtccaaacgg
     2281 acacgtcgga tgaaatttcc aaccttggta atgcggctat cgttagcaga agtcaccaga
     2341 cttccttttt tatccgcatc cgtcaccatg gtacggaact tccaaatctt gaaccgacgg
     2401 ttgtactggg tcacacgctc ttgcttgtaa atcactggcc ccttgctatc caacttgatc
     2461 caaatgctca agatgagaaa gatgggagag gtcagaacca gtaagaccaa ggccaaaaac
     2521 cagtccaagc aacgcttgaa aatcagcgaa cccttccttt tagagacaag atggtagtaa
     2581 gactcaacct cgcttgattt catttccaca ggcaagtctt cccatttcag catactgttc
     2641 tcctttgttt ttattatact atttgtcaac atttttcatt ataccacaaa atggaaaagg
     2701 cggtgaaaga aatctgtaat ttgaaggatt tccttctagt actcaatgaa aatcaaagta
     2761 cagctttgag attgcagata aagctgacat ggtttaaaga gattttcaaa gagtataggc
     2821 catggcccct gcctgcaaac tatacatctt gtgataggtt cctcccagag tcaagagttc
     2881 ctcatgggtt ccactctcga taatgcgtcc cttatccaag acatagatgc agttggcgtc
     2941 ttgaatagta gaaaggcggt gagcgatagc aatagttgtt cggccctgtc tcatcttcgc
     3001 cagagaagct tgaaccaagc tttctgtttc agagtcaata ttggctgtcg cttcatccaa
     3061 aatcaggatt ttaggctggc tggcgactgt tctagcaaag gtaagaagct ggcgctgccc
     3121 agtagagaag ctcgaaccac gctcggaaac aggggagtcg tacccctgag gaagttcttg
     3181 aataaaggaa tctgcatcca caaaggctgc cgcagcctga acctgatcat cactgatttc
     3241 ttggtacatg gcgatattgg acttaatagt tccatgatag aggaagggtt cctgcaagac
     3301 caaaccgatg ttttttctca gctcttcttg actgaaatcc ctgatatcca catcatccaa
     3361 gagaactctc cctgactgga attcataaaa gcgcatgagg acattgataa tagacgattt
     3421 ccctgaacct gtatgaccta caaaggcaat ggtttcaccc ttattaacag agaaagaaat
     3481 gtcatccaga atcggatgtt taccgtcata tgagaaacac acatgttcaa aacggatatt
     3541 gccttcttgg actttggctt gcccattttc ttgaagaggt tcataggtcc tctcatcgat
     3601 taaggcaaag acacggcctg cagaaaccat agacgtttgc agagttgaaa agttttgcgt
     3661 cacctcaatc aaggggtcaa aaaggcggtt gatgtactgg ataaaggcat acatggtccc
     3721 gaccgttatc ccgatagaaa gaccacgata gccaaaatag gccatcaaga cagcataacc
     3781 tagaagtttc aacaaactca tggcaggtct caaaaagagg gcatccaagg ctacagaacg
     3841 gttggcatag accaagtgct cttggttgat ttcatcaaat tctgcctgca ggcgcttctc
     3901 ttgattaaag gcctggatta ttctgattcc ctcgatattc tctgccagct tactattgat
     3961 atctgacaag agacttctgg ttttctcgat gattttcact gacttttttc gatagagatt
     4021 gaccaaaagg aaaatcaaag ggagaaagag caagactaaa gccgtcaaac gaaaatccag
     4081 caccaacatg gtataaaggg ttgtcagaaa gataaaaact gctgagataa agctggataa
     4141 aatcccagaa aacatatcac taatcgtctc ggtatcgttg gtcaaacgag aaacgataga
     4201 acctgctggc gtcttgtcaa agtaagacat gcccagtttc tccatattgg caaaggcatc
     4261 ccgacgaata tccctaacaa tactgtaaga cacgcgcgca aagagaagat tgccgacata
     4321 ctgaactaca gtttgtagga tgtagagacc atagtagacc agcaaaacgg taacggctag
     4381 ttggttaaga ttgctgagat actggtcgat aaagtgggaa gccacgaggg gtatgacact
     4441 tttaatgacc gtcgtcgcta ggagaaaact gagtgccaaa aaggtcagga gtccataagg
     4501 cttgagataa gacatcaagc gcttcaatac agcccattgt tcttgtttat tctgcatctt
     4561 cttctccttt catttccaac tgctgagact ggtaggtttg ggcataccag ccagccatcg
     4621 ctagcaagtc ttcgtgcctg cctctttcga taatttgacc attttgtaga actaaaataa
     4681 aatctgcatg gacaacagca ctgaggcgat gggcagtgat aatggttgtc ttgtcctttc
     4741 gcgtctcctt gaggttgtcg ataatcgcat actctgtctt ggcatctacg gcggataagg
     4801 aatcatctaa aatcaagata tcagggtcta aaatcatagc ccgactcata gccaaccgtt
     4861 gcttttgacc accagaaaga ctgactcctt tttcaccaat cagcgtatca aatccttgag
     4921 gcatgtctac aatatcttgg taaactcggg ctagcttagt agcttccacg gccgctgaaa
     4981 ggggcaagtt aggattgcca aagcggatat tgtctaggat tgaagttgca aagagaaact
     5041 ggtcctgagg aacatagccc atgagactgc gaaggtctgt cagacgatag tcccgaatat
     5101 cgtgaccgtt tagataaatg gcacccttat ccacatcgta ttcacgcaag aggagcttga
     5161 ttaaggacgt tttcccagag cctgtctgcc caaccaagcc cagtgtttgc ccttttgcca
     5221 aactaaagtg aatatccgtc agtgtttcct cattttcaaa agcaaagctg tcaatggtat
     5281 actccaaacg cccattttca ataccatcca gaggaaactc agggtcttgt acaggagatt
     5341 cctgagacaa aagattttca atccgctggt aagaaacctt ccctcgctga gtagtattaa
     5401 agaggaagcc gatggccata agaggccaga ccagcatatc caaatagctg ataaaggtga
     5461 ctagattacc aactgtaatc tgcccttcct gaaccatcaa ggagccaacc aaaagcgtta
     5521 aaacatagga cgaaccaaca aacaagagaa ccatagggtc aaagagacta tcatatttca
     5581 tggtttgcag gttcttttgg aaggttaatt cattgactgc ctgaaaagac ttcaactcgt
     5641 ctgcctgata accgaaagac ttggtcactt tgatacctga tacggactcc tgtaccttgt
     5701 tattgagttc agaaaaagca gcttgggatt cgccaaaggc cttatgagtc tttctcccta
     5761 ggcgactagt cgcataggcc atgaaaggta gggggagaat ggcaacaaga gtcatctgcc
     5821 atgagatgct aaagagcatg gtcaacaaag tcaccagagc cgtgatagag gcatccaccg
     5881 cagacatgac accgccacct gctaaacgag tcaaggcatt gatatcattg gttgcgtgtg
     5941 ccatcagatc acccgtccga taggtttgat aaaaggctga cgacattttt gtgaaatgct
     6001 taaacaagcg agaccgcatg atctgtccca agcaataaga ggtcccaagg atatacatac
     6061 gccacacata gcgcaaatag tacataccaa aggctgcaag tagcaagtaa aataggctaa
     6121 gaaggaggtc ctgctgggtt aattgccccg atgtgatggc atcaatgact cgccccataa
     6181 ccataggagg aatgagattg aggacggaaa ccaagaccag ggccacaatc ccgactagat
     6241 aacggcgttt ttctaacttg aaaaaccacc agagtttttg aataatggac ataaaatccc
     6301 tttctgattg caaatggaaa cctgaggcca atactcaatg aaaatcaaag agcaaactag
     6361 gaaactagcc gcaggctgct caaagcactg ctttgaggtt gtagatagaa ctgacgaagt
     6421 cagtaaccta catacggcaa ggcgacgttg acgcggtttg aagagatttt cgaagagtac
     6481 aagaccccag gtttttctta tttataagtt acgactgtaa cagcaccctt gtcatattca
     6541 gcaataaaga tattggctac attgtcatgc ccttgtttac tgaggttatc aagcaaccac
     6601 tcttcgctac gaccaatcga ttccaagaca tctacttgaa tcacaccgtc agtcacaact
     6661 ggatacttag gattttcatc tcccatttgc acaacgatga gttgcccatt ttgctcttgc
     6721 acagctcgtt tgacttgctt catctggaaa atcccttggc tacgaagttt gagggctaca
     6781 tccgatgcag acaaaccaac tgaacgacag gcttctgggt caatattccc atttttgata
     6841 aggagagttg gcttaccatc aatcaaacgt ttcacaaagt gaacattgtt gttgagctat
     6901 ttgagagtca agaccagttt gattaaaatt tcgatataat taagtgtcat ttgttcacat
     6961 ccaccagttc aattgcgtct gtcttataca attcgacttt ctctaacagg tacttgtctg
     7021 gctcacttcc gtttagggca cgataatagc gatctcccac cttgataagt gcgccatctg
     7081 tgtttgtgga agtattaaca tagacttctg acttgtctac tcccaaatct ttggaaacaa
     7141 cctcgatgaa atgaagtgaa gtttgaaatt tattgttaga aacttgattg ttttgataag
     7201 tcgtgatgct gaccaaaaac agagctaaat aaggtcagag ctgaaatcat gaccaactca
     7261 cgaaattcgg ttcccttttt atcatggtat gccttaaaag caaaaaaaac tgtcacagct
     7321 aatagaacaa tcccaaagcc aaccatgata ccattttgct gaccgatttg gctgagtaca
     7381 tagtcataag agtagaattt catgtatttt cactcccttt cataaaatca ttcttaatta
     7441 taaacgaaag agagtaattt ttcaaactat acgttgggga aatagatctt tttttggtat
     7501 aataaaatct ataatctgaa tgaaaaaggt aactttatga aacttatctc atggaatatt
     7561 gattccctaa acgctgccct aactagtgac tcagctcgtg ccaaattgtc ccaagaagtc
     7621 ctacaaacct tggtcgctga aaatgctgat atcattgcta tccaagaaac caagctttct
     7681 gccaaaggac ctacaaagaa acacgtggaa attttagaag aactcttccc aggctacgaa
     7741 aacacgtggc gttcttccca agagcctgcc cgtaaaggct atgctggaac catgttcctt
     7801 tataagaaag aacttacacc tacgatcagc ttcccagaaa tcggtgcccc ttctaccatg
     7861 gacttggaag gccgtatcat cactctagaa tttgatgcat ttttcgtaac ccaagtttac
     7921 actccaaacg ctggtgacgg tctcaaacgc ttggaagaac gccaagtctg ggatgttaaa
     7981 tatgctgagt atttggctga actagacaaa gaaaaaccag tccttgcgac cggtgactac
     8041 aacgtagccc acaatgaaat cgaccttgca aatcctgcta gcaaccgccg ttcacctgga
     8101 tttactgacg aggaacgtgc tggatttacc aaccttttgg caactggatt tacagacacc
     8161 ttccgccatg ttcatggcga tgttcctgaa cgctacactt ggtgggcaca acgcagcaaa
     8221 acttctaaaa tcaacaatac aggctggaga atcgactact ggctcacaag taaccgtatc
     8281 gctgacaagg tcactaaatc tgatatgatt gactcaggtg ctcgccaaga ccatacaccg
     8341 attgtcttgg aaattgactt gtaaggattt tctatatgga ctacaatgcg gtcattcccg
     8401 agtttcttgt atcgaatatc gaacagtccc ggtccttcta ctgtggttta ctgggtttta
     8461 ggattgaata ccaacgtcca gaagaaaact ttctctttct cttgaagagt gtcaactaat
     8521 gttagaggag ggaacaaagg atcaactagc tgaactgacc tacccctttg gtcgtggagt
     8581 caatctctcc tttggcataa aggatgttcc aaaactctat caaaaagtga tggaggcaaa
     8641 ctatcctatt tatcgtccct tgaccaaaag aaagtttcgt gtcagtgatc cctatatcta
     8701 tcctcataaa tttgcagttt tggatccaga tggctatttt ttaagattta gcgagtagaa
     8761 taataaaaat tgggaaaagt ctttaaagtt agaaaaaggc atattgtcag gtgtctaaaa
     8821 acttgatagt atgcctttta tgatggaaaa atcttctaat actcttcgaa aatctcttca
     8881 agccacgtca acgtcgcctt accgtaggta tatgatactg actctgtcaa ttctatccac
     8941 aacctcaaaa cactgttttg aggttgtgga tagcttccta gtttgctctt tgattttcat
     9001 ttagtataaa tgttagctta caatggagtt tttgttcacc ctctcttctg tttgttcgtt
     9061 ttttaaacca aaacactaac aaaatagttg atttctaaag ttatttagtg taaaataaaa
     9121 acattggctc aagcctattt aaaattgaat atcgttttac ttgtttatgt cgtgaattgg
     9181 cacgacgttt ctacaaggtg ccggaacacc taacaataag taagtcagca gtgagatatg
     9241 gccgtttttg ccatgtctat ttaggggact tacttagaga ttaagtaggt cttttttgaa
     9301 tatttttaat atttccctta attagtgcgg acgggaggtg aaattatcct gtgaataatt
     9361 tcaggaatcc agggaatttc atccctaatt tttgagcccc tagctctttc atttctaggc
     9421 ttaggctaaa atagttcccc gaactatttt actctcaaaa atcccgtcat agacaataac
     9481 aagattattg tctatgatac cactacaacg ggaaatattt gataagactc gcttcgctcg
     9541 taatagtttt atcaaatata tagtattagc aaaagggatc actatagaaa catttaaaca
     9601 acaagtaaat taacttttaa actttaggag gtcttatgaa attattagaa gagcgcatcc
     9661 tcaaggatgg gcatatcttg ggtgataaca tcctcaaggt agattccttt ttaacccacc
     9721 aagttgactt tagcttgatg cgagagattg gtaaggtttt tgcggaaaaa tttgctgctg
     9781 ctggcattac caaggtcgta accattgaag cgtcgggtat tgccccagcc gtttttacag
     9841 ctgaagcctt aaacgttccc atgattttcg ccaaaaaagc taagaacatc accatgaacg
     9901 aaggcatctt aactgctcaa gtctactcct ttaccaagca ggtgaccagc accgtttcta
     9961 tcgctggaaa attcctctca ccagaagaca aggttttgat tatcgacgat ttccttgcta
    10021 atggccaagc tgctaaaggc ttgattaaaa tcatcgaaca ggccggtgcc acagtccaag
    10081 ctatcggtat cgtgattgag aaatccttcc aagatggtcg tgatttgctt gaaaaagcag
    10141 gctaccctgt cctatcactt gctcgtttgg atcgttttga aaatggtcag gtcgtattta
    10201 aggaggcaga tctctaatgc aaactcaaga aaaacactcg caagcagccg ttctgggctt
    10261 gcagcactta ctagccatgt actcaggatc tatcctggtt cccatcatga ttgcgacagc
    10321 ccttggctat tcagctgagc agttgaccta cctgatttct acagatatct tcatgtgtgg
    10381 ggtggcaacc ttcctccaac tccaactcaa caaatacttt gggattggac tcccagtcgt
    10441 tcttggagtt gcattccagt cggtcgctcc cttgattatg attgggcaaa gccatggtag
    10501 tggcgctatg tttggtgccc ttatcgcatc tgggatttac gtggttcttg tttcaggcat
    10561 cttctcaaaa gtagccaatc tcttcccatc tatcgtaaca ggatctgtta ttaccacgat
    10621 tggtttaacc ttgatccctg tcgctattgg aaatatggga aataacgttc cagagccaac
    10681 tggtcaaagt ctcttgcttg cagctattac tgttctgatt atcctcttga tcaacatctt
    10741 taccaaagga tttatcaagt ctatctctat tttgattggt ctggttgttg gaactgccat
    10801 tgctgctact atgggcttgg tggacttctc tcctgttgcg gtagctccac ttgtccatgt
    10861 cccaactcca ctctactttg ggatgccaac ctttgaaatc tcatctattg tcatgatgtg
    10921 tattatcgca acggtgtcta tggttgagtc aactggtgtt tatctagcct tgtctgatat
    10981 cacaaaagat ccactcgaca gcacgcgcct tcgcaacggt taccgcgcag aaggtttggc
    11041 cgtacttctc ggaggaatct ttaacacctt cccttacacc ggattttcac aaaacgttgg
    11101 tttggttaaa ttgtcaggca tcaaaaaacg cctgccaatc tactacgcag ctggtttcct
    11161 ggttctcctt ggactgcttc ctaagtttgg tgcccttgcc caaatcattc caagctccgt
    11221 cctcggcggt gccatgctgg tgatgtttgg ttttgtatct attcaaggga tgcaaatcct
    11281 agcccgagtt gactttgcta acaatgaaca caacttcctt atcgcagctg tttcaatcgc
    11341 tgcaggtgtc ggtctcaaca acagtaatct ctttgtcagc atgccgacag ccttccaaat
    11401 gttcttctca aacggaatcg tcgtagccag cctactcgct attgtcctca atgccgtatt
    11461 aaatcataaa aagaaataag aaaaagaggt gcgagcctct tttttgttta tcacaaattg
    11521 attataaata caatcaatcc caattcccat ctaattgaat tggacctatt tcaaattctt
    11581 taaaatatta tgttccaaat cgttaatatt atataagaat ggtaaaatat caaatgtttc
    11641 tcgtaaatta ttcttagcct tataccaacc ttgtccatca gtaatccaca taaactcaac
    11701 attaggaata gctttagttt cttcagcaat cattttgtaa gatctagccg tctcatttaa
    11761 ttttgaacca cttcctgaat aaaaattaac ctcaatcaga taaagcactt gttcattttt
    11821 tatgacaaaa tcaaaacgtt tcacagtatt cccatcattc gtaatagcag ataaatcgac
    11881 agaaaaaatt tcctctattt cattttgttc aatctcttta aataaattct ctcctaaaat
    11941 atacccttca gcttcaagat agctttgaac aatattttcc atcgcatctc cagtccgatt
    12001 tttgcgccca ttagtatcca taccaacctc aacacctgta acgtagtcaa ctaaatttga
    12061 tactaaatga ttttgcaata aatcaaatat acctgatttt t
//
LOCUS       DAVMEF010000052        12042 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMEF010000052 DAVMEF010000000
VERSION     DAVMEF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.052"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            133..459
                     /locus_tag="VYQ30_001028"
     CDS             133..459
                     /locus_tag="VYQ30_001028"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966166.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVETYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            539..833
                     /locus_tag="VYQ30_001029"
                     /pseudo
     CDS             539..833
                     /locus_tag="VYQ30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632436.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1166..2472
                     /locus_tag="VYQ30_001030"
                     /pseudo
     CDS             1166..2472
                     /locus_tag="VYQ30_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2512..3522)
                     /gene="tsaD"
                     /locus_tag="VYQ30_001031"
     CDS             complement(2512..3522)
                     /gene="tsaD"
                     /locus_tag="VYQ30_001031"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1966167.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWNLNAKPSLAFDT
                     ME"
     gene            complement(3512..3949)
                     /gene="rimI"
                     /locus_tag="VYQ30_001032"
     CDS             complement(3512..3949)
                     /gene="rimI"
                     /locus_tag="VYQ30_001032"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1966168.1"
                     /translation="MIEIKRIQQRPDLAQAIYAVMIAVYPINPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(3946..4629)
                     /gene="tsaB"
                     /locus_tag="VYQ30_001033"
     CDS             complement(3946..4629)
                     /gene="tsaB"
                     /locus_tag="VYQ30_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1966169.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENSKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SVESYIKRL"
     gene            4807..4992
                     /gene="cibA"
                     /locus_tag="VYQ30_001034"
     CDS             4807..4992
                     /gene="cibA"
                     /locus_tag="VYQ30_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1966170.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            4994..5146
                     /gene="cibB"
                     /locus_tag="VYQ30_001035"
     CDS             4994..5146
                     /gene="cibB"
                     /locus_tag="VYQ30_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1966171.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            5964..6197
                     /locus_tag="VYQ30_001036"
     CDS             5964..6197
                     /locus_tag="VYQ30_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1966172.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6199..7878
                     /locus_tag="VYQ30_001037"
     CDS             6199..7878
                     /locus_tag="VYQ30_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1966173.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8230..10143)
                     /gene="mnmG"
                     /locus_tag="VYQ30_001038"
     CDS             complement(8230..10143)
                     /gene="mnmG"
                     /locus_tag="VYQ30_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1966174.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10153..10608)
                     /locus_tag="VYQ30_001039"
     CDS             complement(10153..10608)
                     /locus_tag="VYQ30_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1966175.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEK"
     gene            complement(10749..11870)
                     /gene="mnmA"
                     /locus_tag="VYQ30_001040"
     CDS             complement(10749..11870)
                     /gene="mnmA"
                     /locus_tag="VYQ30_001040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1966176.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3415 a         2829 c         2442 g         3356 t
ORIGIN      
        1 attttctctt gtctcgacct tctccaaaga attgctataa tactattaca aatacatctg
       61 cactacactt caaattttag cactgtataa aaacgtttca atacactaac tttaagaaaa
      121 cttccactat taattgaaaa agttgataga gataaattaa agatctatat tgaaactcat
      181 cccgatgctt atttgactga aatagctgct gaattcaact gtcctccaac aactattcat
      241 tacgctctaa aggctatggg atatagtcta aaaaagagcc gtacctactg cgaacaagac
      301 ccagaaaaag taaatcggtt ccttaaagaa ttgaatcact taagctacct gactcctatt
      361 tatatttatg agacaggggt tgagacctat ttttatctcg aatatgatcg agccttgagc
      421 aggcagttag tctctctgga agaagatata attatttgaa ttaagatcga gacaacgcac
      481 accagagatt gcgatactgt tataggagta ctaatgccct tttttgtttc aatatactat
      541 ggctccgatg acctataaag atacgatgac gagtgacttt ttcgaagctt gcttccaaaa
      601 attcttacta cctactttag atacaccatc ccttatcatt atggacaatg caaggtttca
      661 cagaatgaac atgtgtaagg agcagggcat agactgttac cacttcctac ctattcaccc
      721 gagtataatc ccattgagaa aatatgggct tacatcaaaa acatctcaga ataatattgt
      781 caaattacga tgcttttctt gaggcacttt tgtcctattc ttgtttcagc tgattatact
      841 ccgttattgg gcagctacgg aacagtcgat gggacgatgg ggggacataa aaaaatcctc
      901 cagttttgtt ttttataaca gtatactgga gaattgacaa tctcggtaga tacctcgtta
      961 tagcgcggtt acttattagg cagttacaaa acaactgtga acagaaaaca ttccagagtc
     1021 agacaagact ttggaatgct ttggctctat aatttctgta gtgggtaatc ccaccccagg
     1081 aattataggg tcgtttcttg tagaaaaaaa gccccatatg acctataatg aaaagcgtct
     1141 aaccaactca ttagaaaggg gtcatatgga acaacttaag aataccacag atttgctcgg
     1201 attggaagac aaaaatatca aaatcttgtc tgttctgaaa taccaaaccc atctagtcgt
     1261 tcaggcaaag ttggattccc ccgctcctcc ttgtcctcat tgtcaaggga agatgatcaa
     1321 atacgacttc cagaaagcct ctaaaattcc gcttctcgac tgtcagggtt tacccacggt
     1381 actgcatctc aaaaagcgcc gctttcagtg caagaattgc cttaaggtgg tcgtttctca
     1441 aacatccatt gtcaagaaaa attgccagat ttccaacatg gtgagacaaa aaatcgctca
     1501 gctcctcctt gaaaagcagt ctatgactga gattgcccac agattggcgg tctcaacttc
     1561 caccgtcatc cgaaaactga gggaatttaa gtttgaaacc gattggacca agttgccaaa
     1621 agttatgagt tgggatgagt atagcttcaa aaagagcaaa atgagcttca ttgcccaaga
     1681 ttttgagtcc aaatccatcc tcgcaatttt agacgggcga actcatgcgg tgattcgaaa
     1741 ccatttccaa cgctatcaga gagaggttcg ggagctggtc gaggtcatca ccatggacat
     1801 gtacagccct tattatcggc tcgctaagca actctttcca aaggcgaaga ttgttcttga
     1861 cctcttccac attgtccaac atctgagccg agctatgaac cgagtacgaa tccaaatcat
     1921 gaaccaattt gaccgaaaat ccttggagta tcgggcgctc aagcgctttt ggaaccctcg
     1981 ctttttcgtt tctaggctcg ggctaaatca gtccactgga ctgatttact acaccaggat
     2041 agcttcaagc tctgtcagaa acgattctat cagcccacgt ttcgaatgca cttaacccat
     2101 cgggaagtac gagataagct gctttcttac tctgagggat tacaggttca ctacgaactc
     2161 tatcaactcc tgctctttca ttttcaagag aagaatgccg accatttctt tggattgatt
     2221 gagcaagaac tgccaacggt tcatccgctt tttcaaacgg tcttttggac ttttttaagg
     2281 gatagagata agattatcaa cgcacttaag ctgccttatt ccaacgctaa acttgaagcg
     2341 accaataatt tgattaaggt tatcaagcgc aaagcctttg gtttccggaa ctttaacaat
     2401 tttaaaaaac ggattttgat gactttgaac atcaaaaaag agagtacgaa tttcgtactc
     2461 tccagattgt agcttttcgc ctacccacta cacttgacaa agagccactc tttattccat
     2521 ggtatcaaag gcaagacttg gtttggcatt gaggttccag cctgcgaagt tttctttgtt
     2581 ccactcgctg acactggcat aagcaatcat acctgcattg tctccgcaga ggcgcagagg
     2641 tggaatgatg accttgacat ctgtaacctc ggctgctagg cgttctctga gacctttatt
     2701 ggctgccaca ccacctgcca caaccagggt tttaacagga tatttctcca aagccttctt
     2761 ggtttttgcc atgagaatat ccagtacagc tgcttggaag gaagcacaca aatcctctgt
     2821 agacagactt tctccctttt gctcggcatt gtgatgaaga ttgataaagg cagatttcaa
     2881 acctgagaag gagaactcca gattatcttc cttaatcatg gcacggggga aatcataaat
     2941 atcctgcccc tgatgagcca gctcgtcaat ctcacgacct gcaggatagg tcaagcccat
     3001 gacacgaccg accttatcat aggcctcacc aaccgcgtca tctcgcgttt ccccaacaat
     3061 cttgtaatcg ccagcctcag aaacatagac caactctgtg tgcccaccac tgactaaaag
     3121 ggctagcaag ggaaactcca aaggctccac actctgagct gccatgaggt gcccagccat
     3181 gtgattaaca ggaatcagtg gaagtccgtg agcccaagca aaggccttgg cagctgacaa
     3241 accaactagc aaggctccga ccaagcctgg tccgtaggta accgcaacag ctgtcacgtc
     3301 ctcttcggta atccctgctt ctgccaatgc ctcctcgata caggctgtaa tgacctcgac
     3361 atggtgacga ctggctactt cgggcactac gccaccaaaa cgtttgtgac tctcaatttg
     3421 actagcaatg acattggaca agagctcatc gtcgtttttc aagacggcga cactggtctc
     3481 atcacaggat gtctcaaatg ctaaaatata tctatccttc atctatttct ctcttcatga
     3541 taatggcgtc ctcgactggg tcatggtagt aggcctttcg ctcagcgata actgccatct
     3601 tttctttctt gtaaaatgct tgcgctcgtt gatttgactg tctgacttcg aggaaaattt
     3661 ccttgtctgt cggcaattga gcaaacaagg ctgacgcaat tccctgaccc tgataagctc
     3721 ctttgacagc gatttgcagg acttctgctt caaaaagatt ctcctgcaca gctagaaatc
     3781 caatcacttc tgccccatca taagccaatg cataccaagt ctggtcttgg gacagatctg
     3841 cttggatttg ttccagcgtc caagggttga ttgggtaaac agctatcata acagcgtaga
     3901 tggcttgagc caagtcaggt cgctgttgga ttcgtttgat ttctatcata ggcgtttaat
     3961 gtaagactcg acagactcgg tgtgattctt gagccagttt tcctcagctt cgacacgctt
     4021 gaggtagttc ggcacaaaat catgcaagga gtctgcttcc ttgtcccagg cccatagagc
     4081 tagattagct gcatttggca atgtttcttt gtaatcagtc cttggcaagt gtttttgaat
     4141 ctgctcaaca aaggggccaa cttctccgac aaaggttacc tgactagcgc ccttgattag
     4201 ctcaatgact cgctcaaaag gcagatgtgc ttctgccatg acaggtttgg aattttcata
     4261 aaatcctgca taaacattat tgcgacgcgc atccatcaag gggacaaaca aaccttcttg
     4321 ttggtggggc accagagcca agagactcga cataccaacc aactcgatgt tcagggtgtg
     4381 agctaaggtc ttagcagttg ctaccgcaat tcgcaagcct gtatagctac ccggcccttc
     4441 agctaccacg attcggtcca aatccttggg tgtccaatcc aaacttgcca tcaaaaaatc
     4501 tatggcaggc ataagagtaa tactgtgatt tttcttaata ttaatcgtcg tctcggcaag
     4561 aacctgcttg tcctctaaaa tagcgagaga aagagccttg ctggacgtat caaaagctaa
     4621 tactttcata acacattcct atctttttgt ctgcttacta ttatactaca aaagctggca
     4681 catgggaatt ttctttgccc ccagacaaga gtgccctcac ttaactaaaa ataatttaaa
     4741 aaaatgctca cttttccttt tcttttccga atataaaagt gaacaagaaa aaaggaggaa
     4801 agttcaatga caaattttga cattcttgac aatcaatttt tatccttatc tgaaaatgaa
     4861 ttatcagata ttgatggcgg tctcgctccc ttggttatct ttggagtagc agtatcttgg
     4921 aaggctattg caggtggaac agcacttata ggttctggtt tggcagctgg ttatttttta
     4981 ggaggagatt aatatgatga aaaatttgaa caactatcgt gaaatttcta ataaggaatt
     5041 gcaagaaatc aagggtggct ttggtgtagg tgttggtatc gctttattta tggcaggtta
     5101 taccattgga aaagaccttc gtaaaaagtt tggtaagtca tgctagataa gaaacacatt
     5161 tttagaagga taaattttat tgtcttcatc tcttacagtt tgctcagcat tctcaatgat
     5221 ttgaacatta ctactatccc tttaccattc gatttatctg tttgtattgt tttattttta
     5281 tgcttcaact ctatttttga gcagaacaat gactcccata aaaataataa gctttgaaaa
     5341 ttccattgtc atgtcatttt agaaaaatgc aaagaccacc tcatcttgat agatggggtg
     5401 gaattttcgt gtcgtaaatc tactatctct acatccccaa acaaaaaacc ccagcataag
     5461 cagggcatct aagcatttaa tccaaagtaa aatacaaacc aaacgacata ggtcacgagg
     5521 aggagaaaaa gcgagtagag agtcacaaag gtaattttcc acaagaactt ggtttgtcat
     5581 cgttccagtt tggcaaatag aagatttcct tcataaacgc aagcaacaaa aacgataaaa
     5641 gctagcaaac gaactccgat agcaaaagca aataagttat acatagggca acctccttga
     5701 cttaaaatct atatggaatt atgacaagta ataaacttca cttccgttat caacataaca
     5761 cattttcttt atttttgcaa acgctcacca aagaaatcat cccctaactt tctcgtttcc
     5821 gtcttttact aatttttcat tttgtggtat aattgaaata attgtaacga atcaaggtca
     5881 atctagacac aaaatggaat gaaatcaagc aaatatctgc taaaagtttg gaataagctg
     5941 acctgtaaat agaaaggaac tatatgattt acaaagtttt ttatcaagaa acaaaagaac
     6001 gtagcccacg ccgtgaaaca acacgcgcgc tttacctaga catcgatgcc agctcagaac
     6061 ttgagggccg tatcactgct cgccaacttg tcgaagaaaa tcgcccagag tacaatatcg
     6121 aatatatcga actcttgtct gacaaattgc tcgattacga aaaagaaact ggcgccttcg
     6181 aaattacgga gttctaatat ggcctacact cttaaacctg aagaagtcgg cgtttttgcc
     6241 atcggtggtc taggagaaat cgggaaaaac acttacggaa ttgaatacca agacgagatt
     6301 atcatcgtcg atgctgggat taaattccca gaagatgact tgcttggtat cgactatgtc
     6361 attcctgact actcttacat cgtagacaat atcgaccgcg tcaaggctgt tttaatcaca
     6421 cacggacacg aggaccacat tggtgggatt ccgttcctac tcaagcaagc aaatgtccct
     6481 atttatgctg gaccgcttgc cttggctttg atccgtggga aactcgaaga acacggcctc
     6541 ttgcgcaacg ccaaacttta cgaaatcaac cacaacaccg agttgacctt taaaaatctc
     6601 aaggcaactt tctttagaac gactcactct attccagagc ctttggggat tgtcattcat
     6661 actcctcaag ggaaaatcgt ctgtacgggt gactttaagt tcgactttac tccagttgga
     6721 gaacctgcgg acttgcatcg tatggctgcg cttggtgaag aaggcgtgct ctgtctcctg
     6781 tctgactcga caaatgcgga agtaccaacc tttaccaact ctgaaaaagt cgttggtcag
     6841 tccattatga agattatcca aggtattgaa ggacgtatca tctttgcatc ctttgcctca
     6901 aatatcttcc gtctccagca ggcaacagaa gctgctgtta agactggacg caagattgcg
     6961 gtctttggtc gttctatgga aaaggccatt gtcaacggaa tcgatcttgg ctacatcaaa
     7021 gctcctaagg gaacctttat cgagccaaat gaaatcaaag attatcctgc aggagaagtt
     7081 cttatcctct gtacaggtag tcagggtgag cctatggcag ccctctctcg tatcgccaac
     7141 ggaacccacc gtcaagtaca attacaacca ggtgataccg ttatcttctc ttctagtccc
     7201 atccctggaa acactactag cgtcaacaag ctgattaaca tcatttctga agctggtgtc
     7261 gaagttatcc acggtaaagt gaacaatatc catacatctg gacacggtgg tcagcaagag
     7321 caaaaactca tgctctgctt gattaagcca aaatacttca tgcctgtcca cggtgaatac
     7381 cgcatgcaaa aagtccacgc tggactagca gtggatactg gtgttgagaa ggacaacatc
     7441 tttatcatga gcaatggcga tgtgcttgcc cttactgctg actcagctcg tatcgcaggt
     7501 catttcaacg cccaagatat ctatgtcgat ggaaatcgta tcggtgaaat tggcgcagct
     7561 gtcctcaaag atcgtcgcga tctatctgaa gacggtgtcg ttctggcagt cgcaactgtt
     7621 gacttcaaat cgcagatgat tctatctggt ccagacatcc tcagccgagg ctttgtctac
     7681 atgagagagt ctggcgactt gattcgccaa agccagcgta tcctcttcaa tgccattcgt
     7741 atcgcactga aaaataagga tgctagcgtg caatctgtca atggtgccat tgtcaacgct
     7801 attcgcccct tcctctatga aaataccgaa cgtgaaccga tcatcatccc gatgatcctc
     7861 acaccagatg aagaataaag caagaaaaca gccccgtcct cggagctgtt tttctctatg
     7921 ctttcttttg agattaaaac tcatactcaa tgaaaatcag agagcaaact aggaaactag
     7981 ccgtaggcta ctcaaagcac tgctttgagg ttgtagatag aactgacgaa gtcagtaaca
     8041 tatatacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt atcaataaaa
     8101 atcgaaatca gactagaagg ctaagcgaaa gcataacttg agttagctcc catagttcgg
     8161 ggaactatgg gaggttggag atgaatcaaa gccaagcttt gaactcattc gtaagaagcc
     8221 gacgacgtat cattttgatt tttgaagagt tttagaaata ctacgatttt taccttccag
     8281 atacaccatc aaaatagaaa tatctgctgg gtttactccc gaaatacggc tggcttggcc
     8341 gatggtttct ggattgatga gtttgaactt ctgacgagct tccgtcgcaa tagaatcgat
     8401 gtcatcccag tcgatattgg ctggaatgcg tttttcttcc atacgtttca tcttggcaac
     8461 ctgatccatg gctttggaaa tatagccttc gtacttgatt tctgtttcaa tcaattcgat
     8521 aatcttgtca tccaagtctt ctgcagctgg tccgatgaag gccaccacat cttggtaaga
     8581 aacttctgga cggcgaagga attccttggc tgtcaccgca tctgtcaacg gcttgaagcc
     8641 catctcctca accttggcat tggtttcctt gactggcttg agtttgatac tgtctaggcg
     8701 tttcatctca ttatcaaatt gatttttctt gatttcaaaa cgagcccagc gttcatcatc
     8761 cacaaggcca atctcgcgtc ccatctcagt caagcgcata tcagcattgt catgacgaag
     8821 aatgagacgg tattcagcac gacttgtcaa gagacggtag ggttcaatgg ttcccttggt
     8881 caccaagtcg tcgatcatca ccccgatata accgtcactg cgcttcaaaa tcaactcagg
     8941 cttgccttgg attttcagag ccgcattgat acccgcgata atcccttggc cagcagcttc
     9001 ttcataacct gatgttccat ttgtctgacc agcagtgaag agacctgaga ttttcttggt
     9061 ttccagagtc gcacgcaact gatgaggcaa gaccatatca tactcaatag cataacctgt
     9121 ccgcatcatc tctgcatttt ccaaaccttt gatggaatga accaagtcac gctggacatc
     9181 ctcaggcaga ctggttgaaa gtccttgaac atagacttcc tcagtattgc gcccttctgg
     9241 ctcaaggaag agttggtgac gttccttgtc cgcaaagcgc acaatcttgt cttcaatcga
     9301 cggacagtaa cgaggcccca ctcccttgac cacacctgta aacataggcg cacggtggag
     9361 gttgttttgg ataatttcat gactggtacc attggtgtag gtcaaccagc atggcacttg
     9421 gtccttgaca taatcctcat cacgtgaagt gtatgagaag tgattaggcg cttcatctcc
     9481 tggttggatt tctgtcacgt cgtagttgat agaagaagcc ttgacacgtg gaggggttcc
     9541 tgtcttgaaa cgaccgattt cgaggcccag ttccttgaga ttgtcagcta ggttaataga
     9601 agccaagctg tggttaggac ctgatgagta cttgaggtct ccgatgataa tttccccacg
     9661 gagagcagtc cctgtcgtca caataacagc cttagcagca tattcttgat gggtggctgt
     9721 acgcacaccg acaaccttgc catcttccac caaaatctca tcaatcatgg tttgacgaag
     9781 ggtcagattt tcttgatttt caactgtctt gcgcatctcc ttagagtaaa gttccttgtc
     9841 agcctgcgca cgaagggcac gaacagcagg acccttccct gtgttgagca tcttcatctg
     9901 gatgtaagtc ttgtcaatag tcttggccat ctcgccaccg agggcatcga cttcacgcac
     9961 gacaatcccc ttggcagaac caccgataga gggattacaa ggcatgaaag ccagcatttc
    10021 aatattgatg gtcgcaagca ggaccttaca gcccatacgg ctagcggcca aggaagcctc
    10081 aaccccagcg tgtcccgcac caattacaat aatatcgtat tcttcagtaa aatgatatgt
    10141 catatttctc tcctatttct caagatggat gtgtcttagt tggccttccc aatctggtag
    10201 ggccgttttt aaaaaagctg gaactagctg gatatcctca agcttatcca agtcaatcca
    10261 ctcacagggc tgccttttct catcttcctg catggtcaac ggggcatctt caagcaaatc
    10321 caccagataa tgaaactcga tattgtgata ggaaacgccg tccacttcaa aacgattttc
    10381 aaccacaaaa gctagctgcc cagcttgaga tttaacaccc agttcttcct tcacttcacg
    10441 gactaccgcg tcttccgttt tttcgttaac ttgaatcgca ccgccgataa tgtaatactt
    10501 gcccttgtct ttggtgacta gaagcttgcg attttgaaga atcaaagctg tcgcccgaac
    10561 accaaaaacc gtattgtcta cttttgtccg aaagtcttgt tgagtcattc ttgtcctttc
    10621 ccttaaacga cacaaaaaca gtcaaaacta caaagaagtg caggacaaaa aagcctgcaa
    10681 catccatgag ctttgaccat catttctatt gctgttatta ttgtagcaaa ttgagaaaat
    10741 ttgtcaatct aaatgtactg acaaacttgt ccatcgcggt aagcattgtc aatcaaaccg
    10801 ccacctagac actcttcgcc atcgtaaaag acaactgcct gtcctggtgt aatcgcgcgt
    10861 tgtggttccg caaagataac ttctgccttg tctcctttga catggacggt caccttagag
    10921 tcaggctgac ggtaacggaa tttagccgta cattctagcg taaactcctc gggcatatca
    10981 cgagtaaagt ggacttggct agcctctagg ctagttgaca tgagcgaatc atggtagaaa
    11041 ccttggccta catagagaat attcttgctc aggtcttttc cgacaacgaa ccacggcgcg
    11101 ttgtcaccac cgtgttgtcc accgatacca agcccgccac gctgaccaat cgtatagtac
    11161 ataagacctg catgctcgcc catatcgcga ccatccacag tcatcatgcg accaggctga
    11221 gctggcaggt agttgctgag aaagttttta aagttctttt ctccgataaa gcaaatccct
    11281 gtcgagtctt tcttcttagc agtcgaaaga cctgcttctt ctgctagttt tcgaacttca
    11341 ggctttttca aatgtcctag tgggaacatg gttttttgaa gttgttcttg cgaaagttgg
    11401 ctgaggaaat aggtctgatc cttgccattg tccacgccac gaagcatgtg aacagtgcca
    11461 tcctcatcac gcgccactcg agcatagtgc ccagtcgcta catagtctgc ccccaaggtc
    11521 atggcatagt ccaaaaaggc cttgaacttg atttccttgt tgcacataac gtccggattt
    11581 ggcgtgcgcc ctgcacggta ttccgctagg aaatactcaa aaacgcggtc ccagtactct
    11641 ttttcaaaat tgacagagta gtagggaatg ccaatctgat ctgccaccgc aaccacatcc
    11701 ttgtaatctt cggtcgccgt acagacgccg ttttcatctg tgtcatccca gttcttcatg
    11761 aagataccga tcacatcgta gccctgctcc ttgagcaaaa gagccgtcac cgacgaatca
    11821 acaccaccac tcatccccac gacaacacgt gttttagagt tatcactcat ggtaagtctc
    11881 ccatctattc gtttattcac gattgaaggt cgtgtgtgct tcacgattga aggtcgcttg
    11941 agcagtattc attataacac gcttggttgg agaagacaag gaagagctta aaaattgttt
    12001 tttaaaactt atagtcaata tacagatttg tgaatgatgt ga
//
LOCUS       DAVMEF010000053        11851 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMEF010000053 DAVMEF010000000
VERSION     DAVMEF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.053"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            8..454
                     /gene="argR"
                     /locus_tag="VYQ30_001041"
     CDS             8..454
                     /gene="argR"
                     /locus_tag="VYQ30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1966177.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDKWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            505..3039
                     /gene="mutS"
                     /locus_tag="VYQ30_001042"
     CDS             505..3039
                     /gene="mutS"
                     /locus_tag="VYQ30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1966178.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQVLAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3186..4880
                     /gene="patA"
                     /locus_tag="VYQ30_001043"
     CDS             3186..4880
                     /gene="patA"
                     /locus_tag="VYQ30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1966179.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGNLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5210..5473)
                     /locus_tag="VYQ30_001044"
                     /pseudo
     CDS             complement(5210..5473)
                     /locus_tag="VYQ30_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5671..7437
                     /gene="patB"
                     /locus_tag="VYQ30_001045"
     CDS             5671..7437
                     /gene="patB"
                     /locus_tag="VYQ30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1966180.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7576..8265
                     /locus_tag="VYQ30_001046"
     CDS             7576..8265
                     /locus_tag="VYQ30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1966181.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            8269..8448
                     /locus_tag="VYQ30_001047"
     CDS             8269..8448
                     /locus_tag="VYQ30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966182.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            8435..9784
                     /locus_tag="VYQ30_001048"
     CDS             8435..9784
                     /locus_tag="VYQ30_001048"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1966183.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVIIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            9908..11368
                     /locus_tag="VYQ30_001049"
     CDS             9908..11368
                     /locus_tag="VYQ30_001049"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1966184.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            11382..11816
                     /locus_tag="VYQ30_001050"
     CDS             11382..11816
                     /locus_tag="VYQ30_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1966185.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVSELIPYWWGEERASKFNER"
BASE COUNT         3500 a         2280 c         2702 g         3369 t
ORIGIN      
        1 ggattttatg agaaaaagag atcgtcatca gttaataaaa aaaatgatta ctgaggagaa
       61 attaagtaca caaaaagaaa ttcaagatcg gttggaggcg cacaatgttt gtgtgacgca
      121 gacaaccttg tctcgtgatt tgcgcgaaat cggcttgacc aaggtcaaga aaaatgatat
      181 ggtgtattat gtactagtaa atgagacaga aaagattgat ttggtggaat ttttgtctca
      241 tcatttagaa ggtgttgcaa gagcagagtt taccttggtg cttcatacca aattgggaga
      301 agcctctgtt ttggcaaata ttgtagatgt aaacaaggat aaatggattt taggaacagt
      361 tgctggtgcc aataccttat tggttatttg tcgagatcag cacgttgcca aactcatgga
      421 agatcgtttg ctagatttga tgaaagataa gtaaggtctt gggagttgct ctcaagactt
      481 atttttgaaa aggagagaca gaaaatggcg atagaaaagc tatcacccgg catgcaacag
      541 tatgtggata ttaaaaagca atatccagat gcttttttgc tctttcggat gggtgatttt
      601 tatgaattat tttatgagga tgcggtcaat gctgcgcaga ttctggaaat ttccttaacg
      661 agtcgcaaca agaatgccga caatccgatc cctatggcgg gtgttcccta tcattctgcc
      721 caacagtata tcgatgtctt gattgagcag ggttataagg tggctatcgc agagcagatg
      781 gaagatccta aacaagcagt tggggttgtt aaacgagagg ttgttcaggt cattacgcca
      841 gggacagtgg tcgatagcag taagccggac agtcagaata attttttggt ttccatagac
      901 cgcgaaggca atcaatttgg cctagcttat atggatttgg tgacgggtga cttttatgtg
      961 acaggtcttt tggatttcac gctggtttgt ggggaaatcc gtaacctcaa ggctcgagaa
     1021 gtggtgttgg gttatgactt gtctgaggaa gaagaacaaa tcctcagccg ccagatgaat
     1081 ctggtactct cttatgaaaa agaaagcttt gaagaccttc atttattgga tttgcgattg
     1141 gcaacggtgg agcaaacggc atctagtaag ctgctccagt atgttcatcg gactcagatg
     1201 agggaattga accacctcaa acctgttatc cgctacgaaa ttaaggattt cttgcagatg
     1261 gattatgcga ccaaggctag tctggatttg gttgagaatg ctcgctcagg taagaaacaa
     1321 ggcagtcttt tctggctttt ggatgaaacc aaaacggcta tggggatgcg tctcttgcgt
     1381 tcttggattc atcgcccctt gattgataag gaacgaatcg tccaacgtca agaagtagtg
     1441 caggtctttc tcgaccattt ctttgagcgt agtgacttga cagacagtct caagggtgtt
     1501 tatgacattg agcgcttggc tagtcgtgtt tcttttggca aaaccaatcc aaaggatctc
     1561 ttgcagttgg cgactacctt gtctagtgtg ccacggattc gtgcgatttt agaagggatg
     1621 gagcaaccta ctctagccta tctcatcgca caactggatg caatccctga gttggagagt
     1681 ttgattagcg cagcgattgc tcctgaagct cctcatgtga ttacagatgg gggaattatc
     1741 cggactggat ttgatgagac tttagacaag tatcgttgcg ttctcagaga agggactagc
     1801 tggattgctg agattgaggc taaggagcga gaaaactctg gtatcagcac gctcaagatt
     1861 gactacaata aaaaggatgg ctactatttt catgtgacca attcgcaact gggaaatgtg
     1921 ccagcccact ttttccgcaa ggcgacgctg aaaaactcag aacgctttgg aaccgaagaa
     1981 ttagcccgta tcgagggaga tatgcttgag gcgcgtgaga agtcagccaa cctcgaatac
     2041 gaaatattta tgcgcattcg tgaagaggtc ggcaagtaca tccagcgttt acaagttcta
     2101 gcccaaggaa ttgcgacggt tgatgtctta cagagtctgg cggttgtggc tgaaacccag
     2161 catttgattc gacctgagtt tggtgacgat tcacaaattg atatccggaa agggcgccat
     2221 gctgtcgttg aaaaggttat gggggctcag acctatattc caaatacgat tcagatggca
     2281 gaagatacca gtattcaact ggttacaggg ccaaacatga gtgggaagtc tacctatatg
     2341 cgtcagttag ccatgacggc ggttatggcc cagctgggtt cctatgttcc tgctgaaagc
     2401 gcccatttac cgatttttga tgcgattttt acccgtatcg gagcagcaga tgacttggtt
     2461 tcgggtcagt caaccttcat ggtggagatg atggaggcca ataatgccat ttcgcatgcg
     2521 accaagaact ctctcattct ctttgatgaa ttggggcgtg gaactgcaac ttatgacggg
     2581 atggctcttg ctcagtccat catcgaatat atccatgagc acatcggagc taagaccctc
     2641 tttgcgaccc actaccatga gttgactagt ctggagtcta gtttacaaca cttggtcaat
     2701 gtccacgtgg caactttgga gcaggatggg caggtcacct tccttcacaa gattgaaccg
     2761 ggaccagctg ataaatccta cggtatccat gttgccaaga ttgctggctt gccagcagac
     2821 cttttagcaa gggcggataa gattttgact cagctagaga atcaaggaac agagagtcct
     2881 cctcccatga gacaaactag tgctgtcact gaacagattt cactctttga tagggcagaa
     2941 gagcatccta tcctagcaga attagctaaa ctggatgtgt ataatatgac acctatgcag
     3001 gttatgaatg tcttagtaga gttaaaacag aaactataaa accaagactc actagttaat
     3061 ctagctgtat caaggagact tctttgacaa ttctccactt ttttgctaga ataacatcac
     3121 acaaacagaa tgaaaaggag ctgacgcatt gtcgctccct tttgtctatt ttttaaggag
     3181 aaagtatgct gattcagaaa ataaaaacct acaagtggca ggccctggct tcgctcctga
     3241 tgacaggttt gatggttgct agttcacttc tgcaaccgcg ttatctgcag gaagtcttag
     3301 gcgccctcct tactgggaaa tatgaagcta tttatagtat cggggcttgg ttgattggtg
     3361 tggccgtagt cggtctagtt gctggtggac tcaatgttgt cctcgcagcc tatattgccc
     3421 aaggagtttc atccgacctt cgggaggatg ccttccgtaa aattcaaacc ttttcttatg
     3481 ctgatattga acaatttaat gcgggaaatc tagtcgttcg aatgacaaat gatatcaacc
     3541 agattcagaa cgttgtcatg atgaccttcc agattctttt cagacttccc ctcttgttca
     3601 tcggttcgtt tatcctagcg gttcaaacct taccttctct gtggtgggtg attgttctca
     3661 tggtagtctt gatttttggt ttgactgctg tcatgatggg aatgatgggg cctcgttttg
     3721 ccaagtttca aacccttctt gagcgcatca atgccattgc caaggaaaat ttacgtggcg
     3781 ttcgtgtggt caagtccttt gtccaagaaa aagagcaatt tgctaagttt acagaggtct
     3841 cagacgagct tcttggtcaa aacctttaca ttggttatgc cttttcagta gtggaaccct
     3901 ttatgatgtt ggttggttac ggggcggtct tcctctctat ttggctggtc gcgggaatgg
     3961 ttcagtcgga tccgtctgtt gttggttcca tcgcttcttt tgttaattac ctaagccaga
     4021 ttatctttac cattgttatg gttggatttt tgggaaattc tgtcagccgt gccatgattt
     4081 ccatgcgtcg tattcgagaa attcttgacg cagagccagc tatgaccttc aaggatatcc
     4141 cagatgaaga gttggttgga aaccttagct ttgaaaatgt gacctttacc tatccaatgg
     4201 acaaggaacc gatgctgaaa gatgtgagct ttactattga acctggtcaa atggttggtg
     4261 tagttggagc gactggtgca ggaaagtcaa ccttggctca attgattcca cgtctctttg
     4321 atccacagga aggggctatc aaaatcggag gtaaggatat tcgagaagtg agtgaaggaa
     4381 ccctgcgtaa aacagtttcc atcgttctcc aacgtgccat tctttttagt ggaacgattg
     4441 cagataactt gagacagggg aagggaaatg ctactctatt tgaaatggag cgcgcagcca
     4501 atattgccca ggctagtgaa ttcattcatc gtatggagaa aacctttgaa agtccagttg
     4561 aagaacgggg aaccaatttc tctggtggac aaaaacaaag gatgtcgatt gcgcgtggga
     4621 ttgtcagcaa tccacgtatt ctgatttttg atgattcgac ctcagccttg gatgccaaat
     4681 cagagcgctt ggtgcaagaa gctttgaata aggacttgaa ggggacgaca accattatta
     4741 ttgctcaaaa aattagctcg gttgtccatg cagacaagat cttggttcta aatcaaggac
     4801 gattgattgg tcaaggcacg catgcagact tggttgccaa caatgccgtt taccgtgaaa
     4861 tctatgaaac acagaaatga aagacaaact ataagaaaag tcaatagttt tatctaaact
     4921 atttcttatt tcaatttgat gatttggcga tgattttaga gcacggcaaa aagctattga
     4981 aaaagtccat tttttcaaag gtaatcctgt gttaatttca gaaattacat cactttttgt
     5041 tcgtcaaatg gcagctcttt tttaggatat aaaacagggt tcggataagt ttttttgcaa
     5101 ggtggatgat ggctacattg taatgttttc cttgttctaa cttagtctta agataggcct
     5161 taaaagcagg tgaaaagcga gggcatgctt tggcagcttg tatgagtacc taccgcagat
     5221 gaggggaacc ccgtttgacc attcttcctg ctaaatcaat ctgacctgac tgataaatag
     5281 aagaatccag tccagcgaaa gcttgtaatt gagcaggatt atcaaaggca tgaatatttc
     5341 gaatctcggc taaaatgacc gcccctaaac gattctcaat cccagtaacc gtcgtgatga
     5401 ccgagttgaa ctcagccatc aagtcattga cacatttttt cgccttgtca atgagcctct
     5461 tgtaatgttt gatgttttca ttacaagaga taaaacgttt atgcgttatc aaactcatta
     5521 ccaattgaaa caaaaagctg tggttagatc ctttcggaaa ttgtcaagcg attggaggaa
     5581 atgaactaat ccacagcggc ttattccaag tataccactt gggctttggc agtagctaac
     5641 tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt ttggcattat
     5701 tttaaggtct acaagttctc atttgtagtt gtcatcctga tgattgttct ggcgactttt
     5761 gcccaagccc tctttccagt cttttctgga caagcggtga cgcagctagc caatttagtt
     5821 caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc
     5881 aatcttggcc tgctggtttt ggttctattt atctctagtg taatatacat gtgtctcatg
     5941 acgcgcgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg taagcttgct
     6001 cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcctgtc tcattttacc
     6061 agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat
     6121 attgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc
     6181 atcaccattg ccagcacccc attggctttc cttatgctga ttttcatcgt gaaaatggca
     6241 cgtaaataca ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag
     6301 agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga
     6361 tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga
     6421 attcttttcc ctgtcatgaa tgggatgagc ctgattaata cagccatcgt catctttgct
     6481 ggttcggctg tacttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt
     6541 gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt
     6601 tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca
     6661 gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa
     6721 atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga tgtcagcatt
     6781 tctgccccta aaggccagat gacagcagtt gttgggccta caggttcagg aaaaacgact
     6841 attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt
     6901 aaagacattc gtggctatga cttagatagt cttagaagca aggtgggaat tgtattgcaa
     6961 gattcggtct tgtttagcgg aacgattcga gacaatatcc gatttggtgt gccagatgct
     7021 aatcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt
     7081 ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc aacagggcag
     7141 aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat
     7201 gaagcaactt caaacgtaga tacggtgacg gaaagcaaga ttcagcatgc catggaggtg
     7261 gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca
     7321 gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt
     7381 ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa
     7441 agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta
     7501 aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc gttagtattc
     7561 gaaaggagag gcatcatggc tagaacggtt gtaggagttg ctgcaaatct atgtcccgta
     7621 gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt
     7681 caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt acgtgattat
     7741 gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc tcagttttat
     7801 ggagagaaaa agaccgtcga aagcgatgat tacaatctgg tccgtgacga atttgaattg
     7861 gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc
     7921 caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa
     7981 ggactacctt tcgggacatc tcactctatt gagacagtag aaggaagcgt ggtggctaag
     8041 ctatttggga aagaaagtca ggtcaactca gtccatcgtc aaagcattaa agatttggca
     8101 cctaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct
     8161 atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa
     8221 gatggcaatt tagaattatt tgagtattta ttgaatgaac tgtaacgatt ggaacatctg
     8281 gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gcattttacg caaacgtttg
     8341 aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt
     8401 tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc
     8461 aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac
     8521 agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt
     8581 ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca
     8641 aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc
     8701 aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg
     8761 caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactt
     8821 ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac
     8881 aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca
     8941 agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt
     9001 tgaagcagac gctaacggtt ggggaacatt tgttgttcca gatgatatcg gtggacgctt
     9061 ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg acataaaagc
     9121 tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa
     9181 cgaagcttac caatacgcag ctgttcgtaa catcctttat cgtaaaggct atgcaactga
     9241 gatcttggta aactatgagc catcacttca atacttctca gaatggtgga aacaattggc
     9301 tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac
     9361 tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt
     9421 tgtccgtgtt gacaaacctc gtaaaaacgt gattattcct actttggaag aagaccttga
     9481 cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg
     9541 tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca
     9601 agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg
     9661 ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat
     9721 gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta acgcacgtct
     9781 ataatagaag aaaagagtgg tttgcccact ctttttactc tctttatcca tagaaattgg
     9841 actcagccaa gacttgtgat ataatataga aagcaaaaag gcagacgcct agataatagg
     9901 agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca
     9961 catcggaaat gcccgtacag cattgtttaa ctacttgtat gcgcgccatc atggtggaac
    10021 atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc
    10081 acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga
    10141 gaattatcgc cagtctgagc gtttggactt gtatcaaaaa tacattgacc aactattagc
    10201 tgaaggaaaa gcctataaat cttacgttac agaagaagag ttggcagctg aacgtgaacg
    10261 tcaagaagct gctggtgaaa cacctcgata tatcaacgaa taccttggta tgagtgaaga
    10321 ggaaaaagcg gcttatatcg cagaacgtga agcagctggt atcataccaa cagttcgttt
    10381 ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggtg atatcgaatt
    10441 tgaaggtggc aatatcggcg gtgactgggt tatccaaaag aaagacggtt acccaactta
    10501 caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta tccgtggaga
    10561 tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg gttgggaagc
    10621 tccagagttt ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa
    10681 acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga
    10741 agcagtcttt aactttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt
    10801 ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc
    10861 ctttgatcag aaaaaattag actggatgag caatgattat atcaagaatg cagaccttga
    10921 aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc
    10981 tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt
    11041 gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg aagtcatgac
    11101 gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga
    11161 tgatgaattt gtgacagaaa atatcttccc acaaattaaa gcagtccaaa aagaaacagg
    11221 tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg
    11281 gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga
    11341 aaacattcta aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga
    11401 tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca
    11461 atcatgttgt agctgcagta gaagcaggag atggacaaat atttacagga ttctgtatgg
    11521 agggaacctg tggtgttttc catctctgcg cagaacgggc ggccctcttc aatatgtacc
    11581 aattttcagg acaaactaag gttaagaaag tattagcctt tcgagataaa ccaccttatg
    11641 gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagag cttaatgctg
    11701 aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt aaagtctcag
    11761 aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag cgatagaaga
    11821 gtcagtataa ttctggctct ttttactata a
//
LOCUS       DAVMEF010000054        11524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMEF010000054 DAVMEF010000000
VERSION     DAVMEF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.054"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            111..764
                     /locus_tag="VYQ30_001051"
     CDS             111..764
                     /locus_tag="VYQ30_001051"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1966186.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            774..1238
                     /locus_tag="VYQ30_001052"
     CDS             774..1238
                     /locus_tag="VYQ30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1966187.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1251..2519
                     /locus_tag="VYQ30_001053"
     CDS             1251..2519
                     /locus_tag="VYQ30_001053"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1966188.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2533..2793
                     /locus_tag="VYQ30_001054"
     CDS             2533..2793
                     /locus_tag="VYQ30_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966189.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2804..4165
                     /gene="pdrM"
                     /locus_tag="VYQ30_001055"
     CDS             2804..4165
                     /gene="pdrM"
                     /locus_tag="VYQ30_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1966190.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGDFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSMVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPEFIDLTACFLTYSLFFQLADTFAAPIQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4319..5287
                     /locus_tag="VYQ30_001056"
     CDS             4319..5287
                     /locus_tag="VYQ30_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1966191.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNVEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQTQVKEVVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5303..6295
                     /locus_tag="VYQ30_001057"
     CDS             5303..6295
                     /locus_tag="VYQ30_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1966192.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6513..7556
                     /locus_tag="VYQ30_001058"
     CDS             6513..7556
                     /locus_tag="VYQ30_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1966193.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     NTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKAEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7620..9305
                     /gene="lpdA"
                     /locus_tag="VYQ30_001059"
     CDS             7620..9305
                     /gene="lpdA"
                     /locus_tag="VYQ30_001059"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1966194.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9369..10358
                     /locus_tag="VYQ30_001060"
     CDS             9369..10358
                     /locus_tag="VYQ30_001060"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1966195.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANITESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10431..11501)
                     /gene="xerS"
                     /locus_tag="VYQ30_001061"
     CDS             complement(10431..11501)
                     /gene="xerS"
                     /locus_tag="VYQ30_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1966196.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3392 a         2171 c         2608 g         3353 t
ORIGIN      
        1 ttcaaatcgt atatatagtc gatttattaa atctttagat aaactgtaaa aagtggtttc
       61 catagccact ttttgctata atagaggcag taaaaatgaa ggagtcggct atggaacact
      121 cgtcttggca tgctttgatt aaggcgcaat tacctgaggg ttatttcggg aaaatcaatc
      181 agtttatgga gcaggtctat tctcagggga ttatttatcc acccaaggaa aaggtttttc
      241 aggctctctt gacaacactg cttgaagaag ttaaggtggt aattctaggg caagacccct
      301 atcacggacc aggtcaagcg cagggcttga gtttttctgt acctgactct atcccagctc
      361 caccatcctt gcaaaatatc ttgaaagaat tgtcagatga tatcggagtt aagaaatctc
      421 atgatttgac agcttgggct gagcaaggag tcttgcttct taatgcttgt ttgactgttc
      481 ctgctggaca ggccaatggt catgctggtc aaatctggga gccttttact gatgctgtga
      541 ttcaggtggt caatcatcta gatagaccag tcgtttttgt actctgggga gcttatgcac
      601 gtaagaagaa ggcattagtt accaatcctc atcacttgat tatcgaatca gcccatccaa
      661 gtcctttgtc ggtttataga ggattttggg gttccaagcc cttttccaag gccaatgcat
      721 tcttaaaaga gacaggacaa gagccaatcg attggcttag ataaggagaa aatatgcctc
      781 agttagcgac gatttgctac attgataatg ggaaagaact gctcatgctc catcgtaata
      841 agaaacccaa tgatgtccat gaagggaaat ggattggtgt gggtggtaag ctagagagag
      901 gagagacgcc ccaggaatgc gcggcgcgtg aaatccttga agaaacaggg ctcaaagcca
      961 agccagttct aaaaggtgtc atcacttttc ctgaatttac accagattta gactggtaca
     1021 cctatgtttt taaggtgacg gagtttgagg gcgacttgat tgactgcaat gaggggacgc
     1081 tagaatgggt tccctatgat gaggttttga gcaagccgac ttgggaaggt gaccacacct
     1141 ttgttgagtg gcttttagag gataaaccct tcttttcagc caagtttgtt tatgatgggg
     1201 ataaattgtt ggatacccaa gttgatttct atgaataaag gagaaagcag atgctactaa
     1261 tcaaaaatgg tcgtgtaatg gatcccaagt ctggtttgga tcaagtttgt gatgtcttag
     1321 ttcaagatgg gaaaattatc aaaattgcgt ctgagatcac ggaagaagga gcagaaacga
     1381 ttgatgctac tggtcttgtg gttgctcctg gcttggtcga tattcatgtt catttccgtg
     1441 aacctggtca aacacataaa gaagacattc atactggtgc cctagcagcc gctgcaggtg
     1501 gttttactac tgtcgtcatg atggctaata ccagtccaac catttcagac gtggagactt
     1561 tgcaagcagt tctccagtca gctgccaaag agaagattaa tgtcaagaca gttgcgacca
     1621 ttactaaaaa ctttaatggt aaaaacttga ctgactttaa ggcactctta gaagctggtg
     1681 cggttggttt ctctgatgac ggtattccgc ttgagagcag taagattgtc aaggaagcca
     1741 tggaggaagc caaaaaactc aataccttta tcagccttca tgaggaagat ccaggtttga
     1801 acggtgttct tggctttaat gaaaatattg ctagagaaca tttccatatc tgcggtgcta
     1861 ctggagtggc tgagtacgct atgatggcgc gtgatgtcat gattgcctat gcaactaaag
     1921 cccatgttca catccagcat ttgtctaagg aagaaagtgt taaagtagtg gagtttgctc
     1981 aggggttagg tgcagaagtc acagcagaag tagcaccaca gcatttctct aagacagaag
     2041 cacttctttt aacacaaggt agcaatgcta agatgaatcc accgcttcgt ttggaatcag
     2101 accgtcgtgc cgttatcgaa ggtctcaaat caggtgtcat cacagttatt gcgactgacc
     2161 acgcgcctca tcatgtagat gaaaaaaatg ttgaagatat taccaaagcg ccatctggta
     2221 tgactggctt agaaacatcc ctgtctctcg gcttgaccta tttggtagaa gctggtgagt
     2281 tgagcttgat ggaattactt gaaaaaatga catacaaccc agccaagctt tacaacttta
     2341 aagcaggtta cttggctgag aatggtccag cagatatcac tatttttgat gccaaggctg
     2401 accgccttgt ggactcccat tttgcttcca aagcagctaa ttcaccattc atcggtgaaa
     2461 ccttaaaagg gcaggttaaa tataccatct gtaagggaca aatcgtctat caagcttgat
     2521 aggagttaga atatgaatta ttttcgaaaa cgtagggaga gacaagcgaa atctaatagt
     2581 ggaatttact gtcccgcagc taatcgttcc aaaattcaat atgaaactaa agaaaaggct
     2641 gaccatgcgg ttcaatatga taacggaggt ttggttagaa gctattattg tcgtacatgt
     2701 gcgtgttggc atactacttc aaaatctaat aaacctccgc taagtttgaa aaactatggt
     2761 ctgaaactct ttggtttaga taaatcagaa tagaaagaga agtatgtata agacaaagtg
     2821 tttacgagag aagttggtat tatttttaaa aattttcttc ccaatcctga tttaccaatt
     2881 tgccaattat tctgcctctt ttgttgatac tgcaatgaca ggtcaataca acactatgga
     2941 cttggctggt gtatctatgg caaccagtat ctggaatcct ttctttacat ttctaacagg
     3001 gattgtgtca gccttggtgc ctatcattgg tcaccatctt ggtcgaggaa aaaaggaaga
     3061 agttgcgtct gatttttacc aatttattta tttggccttg ggcctatctg tggtcttgct
     3121 ggggatggta cttttcttgg caccaacaat cttgaatcat attgggttag aagcagcagt
     3181 agcggcagta gcggttcgct atctttggtt tttatctatc gggattatcc ccttgttgct
     3241 ctttagcgtc attcgttcct tgctggattc gctgggcttg accaaactgt ccatgtacct
     3301 catgcttttg ttactccctc tcaatagcgg atttaactat ctcttgattt acggtgactt
     3361 tggtgttcca gaactgggag gggctggtgc tggtttagga acatccttgg cctactgggt
     3421 cttgcttggg atttctgttc tggttttatt taaacaggag aagctcaaag ccttacacct
     3481 tgagaaacga attccactta atatggataa cattaaggaa ggagttcgtt taggtctgcc
     3541 tattggggga actgtcttcg cggaagtggc tatcttttca atggttggct tgattatggc
     3601 taagttttcg tccttgatta tagctagtca ccagtcagct atgaactttt caagtcttat
     3661 gtacgccttt cctatgagta tctcatcggc tatggctatt gtcgtttcct atgaagtggg
     3721 agccaagcga tttgatgatg cgaaaaccta tattggtcta ggaagatgga ctgccctcat
     3781 ttttgcggcc ttcaccttaa ccttccttta catttttagg ggaaatgtgg ccagtcttta
     3841 tggtaacgac ccagaattta tcgatttgac agcgtgtttt ttaacttata gtcttttctt
     3901 ccagttagca gatacctttg cggcaccgat tcagggaatt ttgcgggggt ataaggatac
     3961 agttattcct ttttaccttg gtttgcttgg ttattggggc gtagcaatcc ctgtggctac
     4021 gctatttgat tccctaacag attttggagc ttattcttac tggatcggct tgattattag
     4081 tttgattgtg agtggggcgc tctaccgttg gcgtttaact gtgattatga agagatttga
     4141 atctttagca aaatccaaac gctaaaaagt ttgtgaaaaa atattcttga caagaaaatc
     4201 ccttgttttc acggggtttt cttttttatt ttgtgaaatt tcttttagaa aaactttgac
     4261 agtatttgcc aaaaacggta aaatagtaag gtaagaagaa agttagaaag gcaagaagat
     4321 gtcaacttta gataaaaatc ttttgctaga aatgttccgt aagatggaag aaatccgtcg
     4381 catggactta aaaattgcac aattagtaaa gaaagggaaa gtgccaggaa tgacgcactt
     4441 ttctgttggt gaagaggcag ctaacgtggg ggctatgtta gctctcaatc cagatgatct
     4501 gattacctca aaccaccgtg gacacgggca agctattgct aaagggattg acctcaacgg
     4561 aatgatggct gaaatccttg gtaaatacac tggaacctgt aaagggaaag gtggatctat
     4621 gcatatcgct gaccttgatg ctgggaacct tggtgccaat ggtatcgtag gtggtggtat
     4681 ggggatcgct gtcggtgcag ccctcagtca gcaaatgcaa aataccggta aaatcgttgt
     4741 ctgcttcttt ggagatggtg cgaccaatga aggtgttttc cacgaagcag tgaacatggc
     4801 ttctatctgg aacctgccag tcattttcta ttgcattaac aacggttacg gtatctctgc
     4861 ggatatcaag aaaatgacca atgtagaaca tatccatcaa cgtagcgccg cttatggaat
     4921 tcctggaatg ttcatcgaag acggtaacaa tgtcatcgat gtctatgaag gatttcagaa
     4981 agctgtagac catgttcgca gtggcaatgg tccagtcttg attgaaagtg taacttatcg
     5041 ctggcttggt cactcatcat ctgaccctgg taaatatcgt acgcgtgaag aagtggaatt
     5101 gtggaaacaa aaagatccaa tcgaaaacct ccgcaattac cttattgaaa ataacattgc
     5161 aagtgccgaa gaattggaag aaatccaaac gcaagtaaag gaagtagtag aagcttctgt
     5221 taaatttgca gaggaaagcc cattcccacc gcttgaatca gcctttgaag atatttacgc
     5281 agactaagga gaaagagata aaatggaaac aaaaacaatg tccttccgtg acaccattat
     5341 ccttgctatg tctgaggaaa tgcgtcgcga tgaaaatgtg ttcttgatgg gagaagatgt
     5401 cggtgtcttc ggaggagact tcggtacttc tgttggaatg cttgaagaat ttggtccaga
     5461 acgtgtccgt gactgtccga tttctgaagc tgccatctct ggagcagcag caggagcagc
     5521 catgacagga cttcgtccaa tcgtcgatat gaccttcatg gacttctctg ttattgccat
     5581 ggacaatatc gtcaaccaag ctgctaaaac acgttacatg tttggtggta aaggtcaggt
     5641 tccaatgact gttcgttgtg cagctggtaa cggagttggc tctgccgccc agcactcaca
     5701 atctctagag tcttggttta ctcacattcc aggacttaag gttgtggcac ctggaacacc
     5761 tgcggacatg aaaggcctgc ttaagtcttc tattcgtgat aacaacccag ttattatact
     5821 tgagtacaag tcagaattta accaaaaagg ggaagtgcca gttgatccag actacacaat
     5881 tccacttggg gttggtgaaa tcaaacgcca aggaacggat gtaacagttg ttacttatgg
     5941 aaaaatgctt cgccgtgtgg ttcaagctgc tgaagaatta gcagaagaag gaatttcagt
     6001 tgaaattgtt gacccacgta cccttgttcc gcttgataag gatatcatca taaactcagt
     6061 gaagaagact ggtaaggttg ttctggtcaa cgatgcccac aaaacaagtg gctatatcag
     6121 tgaaatttca gctattattt cagaatcaga agcatttgac tatctagatg caccaatccg
     6181 ccgttgtgca ggagaagatg tgccgatgcc ttatgcacaa aacctagaaa atgcaatgat
     6241 tccaacagtt gaaagtatca aagatgcaat ccgaaaaact tataacaaag aatagagtta
     6301 cataagataa gttatgtaaa taaaataaag ctgacgagaa actttgtatc ttttaggtgc
     6361 agagctcttc gtcattaata tcttatattc gagcacgggc taaaagctcg gaaaaaagat
     6421 aaatctcctt ggcttcatcg aagccatcgt cgatttccta tttttcagtc gcttttgacg
     6481 ccctttgtat cataaataga attggagagg tcatggctga tgacaagcta agagcgactc
     6541 ctgcagctag aaagttagcg gatgatttag ggataaacct ctacgacgtt tctggctcag
     6601 gtgcaaacgg tcgtgtccac aaagaagacg tggaaactta taaaaacaca aacgtggttc
     6661 gcatttcgcc acttgcaaaa cgaattgccc tcgaacataa cattgcttgg caggaaatcc
     6721 aaggaaccgg tcatcgtggt aaaatcatga agaaggatgt tttggccctg cttcctgaaa
     6781 atatcgaaaa cgatagcatc aaatctcctg ctcagattga aaaagcggaa gaagtccctg
     6841 ataacgtaac accatatggt gaaatcgagc gtattccaat gacaccaatg cgtaaggtta
     6901 ttgcccaacg catggttgaa tcttacttga ctgcgccaac cttcaccctc aactatgaag
     6961 ttgatatgac tgaaatgttg gctcttcgta agaaggttct tgagccaatc atggaagcaa
     7021 ctgggaagaa gactactgta acagaccttc tttcgcttgc agttgttaag actcttatga
     7081 aacacccata catcaacgct tcattgacag aagatggcaa gactattatc actcacaact
     7141 atgtcaatct tgccatggca gttgggatgg ataatggatt gatgacacct gttgtttaca
     7201 atgctgagaa gatgagtctt tcagaactgg ttgtagcctt taaggatgtt attggccgta
     7261 ccttggatgg taaattggct ccaagtgagt tacaaaattc aacattcaca atcagtaatt
     7321 tgggaatgtt tggtgttcag tcctttggtc cgattattaa ccaacccaac tcagctatcc
     7381 ttggtgtcag ttcgacaatc gagaagccag ttgtcgtcaa tggtgaaatt gtgattcgcc
     7441 caatcatgag tttaggatta accattgacc accgtgtcgt agatggtatg gctggtgcta
     7501 agtttatgaa ggacttgaaa gagttgattg aaaccccaat ctcaatgttg atttaagagc
     7561 aagagtattc atttttaaga tatagataaa ggaaggaaga catggcctta gaagtaatta
     7621 tgccaaaagc cggcgtggat atgacagaag gacaaatcgt ccaatggaat aaaaaagtcg
     7681 gagaatttgt aaaagaagga gaaatccttt tggaaatcat gactgataaa gtcagcatgg
     7741 aattggaagc cgaagaagat gggtacttga ttgccattct caaaggagat ggtgaaactg
     7801 tccctgtaac ggaagttatc ggttaccttg gtgaagaaag ggaaaacatc ccaacagctg
     7861 gagcagcatc gccagaagct agccctgtac ctgtagcaag tacctcaaac gatgatggta
     7921 agagcgatga tgcttttgat atcgttgtga ttggtggagg tcctgctggt tatgttgcag
     7981 ccattaaagc tgcccaactc ggcggtaagg ttgcccttgt tgagaaatct gaacttggtg
     8041 gaacctgctt aaaccgtgga tgtattccaa ccaagaccta ccttcataac gctgaaatta
     8101 ttgaaaatat cggtcatgct gcaaatcgtg gtatcgtgat tgaaaatcct aacttcactg
     8161 ttgatatgga aaaactttta gaaactaaat ctaaagttgt taatactctt gttggtggtg
     8221 ttgcaggact tcttcgtagc tacggagtta ctgttcataa aggaattggt acaatcacta
     8281 aagacaagaa cgtcttggta aatggttctg aattgcttga aaccaagaaa atcattcttg
     8341 ctggtggttc aaaagtcagc aagatcaacg tccctggtat ggaatctcca cttgtcatga
     8401 ctagtgatga cattcttgaa atgaacgaag tgccagaaag ccttgttatc atcggtggtg
     8461 gagttgtcgg tatcgaactc ggtcaggcct tcatgacatt tggttcaaaa gtgactgtta
     8521 tcgaaatgat ggaccgtata gttccagcta tggatgtgga agtttctaag aaccttcgct
     8581 tgatccttga gcgtaaagga atgaccatct tgactggtac taaactgcaa gaaatcattg
     8641 aggaaaatgg tcaacttcgt atcaaggttg aaggaaaaga cgatatcatc gcaagcaaag
     8701 ctcttctttc aattggtcgt atgccagacc ttgaaggtat tggagaggtt gagtttgaat
     8761 tggatcgtgg ttgtatcaag gtcaacgaat acatggaaac ttcagttcca ggcatttatg
     8821 caccaggtga catcaacggt actaagatgt tggctcacgc agctttccgc atgggtgaag
     8881 tttccgctga aaatgccctt aaaggaaatc atgcagttgc caaattgaat ttgactcctg
     8941 cagccatcta cactctccct gaagtagcag cagtaggttt gacagaagaa caagcccgtg
     9001 agaaatacga tgttgccatc ggtaagttta actttgctgc taacggtcgt gctattgcat
     9061 ctgacgcagc tcaaggtttc gtaaaagtta tcgctgataa gaaatacgga gaaatccttg
     9121 gtgtacacat tattggtcct gcagccgcag aattaataaa cgaagcatca agcatcatcg
     9181 aaatggaaat cactgttgag gaaatgctga agaccatcca cgggcaccca acttactctg
     9241 aagtgatgta cgaagcgttt gcagatgttc taggaatggc catccattca cctaagaaaa
     9301 aataaaacga agatagatta gactggaagg ttatttccag tttctatcat ataaagtaaa
     9361 gggatatcat gaaatacatt atcaatcatt caaatgacac tgcttttaat attgccttgg
     9421 aagaatacgc ctttaaacac cttttggatg aggatcaaat cttcctactt tggattaaca
     9481 agccatctat cattgttggt cgtcaccaga acactatcga agaaatcaac cgtgattatg
     9541 ttcgcgaaaa tggtattgag gtagttcgcc gtattagtgg tggtggagct gtttaccacg
     9601 atttaaataa cctcaattac acgattatct ccaaagaaga tgaaaataag gcatttgact
     9661 tcaagagctt ctcaactccg gttatcaata ccctggctca acttggggtt aaagctgagt
     9721 tcacaggtcg taatgacctt gagattgatg gtaagaagtt ctgtggcaat gcccaagcct
     9781 atatcaacgg ccgtatcatg caccacggtt gcttgctctt tgacgttgat ttgtcagtcc
     9841 tcgcaaatgc ccttaaggtt tcaaaagata aatttgaatc aaaaggtgtg aaatccgtcc
     9901 gtgcccgtgt aaccaatatt atcaatgaat taccaaaaaa aatcacagtc gaaaaattcc
     9961 gtgatttact cttggaatac atgaaaaaag agtacccaga gatgactgaa tacgtctttt
    10021 cagaagaaga attggccgaa atcaatcaca tcaaggatac taagtttgga acttgggact
    10081 ggaactatgg taaatcacct gaatttaacg tccgtcgtgg aaccaaattc actagtggta
    10141 aggttgaagt ctttgctaac attactgaat caaaaatcca agacatcaag atttatggtg
    10201 acttctttgg tattgaagac gttgctgcag tagaagatgt ccttcgtggg gtgaaatacg
    10261 aacgcgaaga tgttcttaag gcgctaaaaa ccattgatat cacacgctac ttcgctggta
    10321 ttagccgtga agaaatcgct gaagcggtag ttggataaat caaaaagaag ttggccaact
    10381 aaccaacttc tttttttgat atttatttac ataatttaaa atacgtaaaa tcataaacta
    10441 tccagagcat tcttttgttc atcattaaca atatgggtat agaggtcagt gacttgtgtg
    10501 ctggcatgtc ctagttggtg actgactaaa acttgtgatt tagtcgcatc atagagccta
    10561 gttgctagtg tatggcgcag tttatggggt gttacacgta ctttaaaatc ctctgagtat
    10621 ttagcaacca ttttctcaac gctagaagca tcgatacgat taggaacacc tctgtagaga
    10681 gttaaaaaaa gggctgtatc tgttttttcc gttttatagc gttgattccg aatggccaga
    10741 taattctcta aataaggttt agcaaaagca gcgacattga ctgagtcacg tttgccacct
    10801 tttcgagtaa catcaataac catcattttt agattgagat ctcttagatc tagattaaca
    10861 gcttcagata agcgaacacc agatgccaag agaagggcaa taatggctaa atctcgttct
    10921 ttatttttgt tgaatgatga gagagctcga tttgaaagct gttgtgggtg ctcttgatcg
    10981 atataagtta gaaaaccttc tgtttcatca cctagaaaga gtttttgctt gatattttca
    11041 gctctggcag caagggtttc tttctttttc ttggttgaaa cttttttcat tacattacga
    11101 tagaaataag gttccccctg atcgttttca acctcctcgg ttagatactt gtaaagacta
    11161 gaaagtgctg ataaggttcg attgatagtt gtctgtgaaa caccttgttt tgttgtatta
    11221 gcattcagca agggacgttc acgtagataa aggataaagg attccatgtc tttcttagac
    11281 atattttcca aaactgataa aggaatatcg gatattttat cagcgtttga aataccagac
    11341 tccaaaaccc agctgaaaaa tcggtcatat tccttaaggt attcgtacag ggttgtaaaa
    11401 ctgtagggca cagccagctt agattggtag tattccagaa cataccaggg catgagttgt
    11461 tttagtttgt cgattcgttc cagtaaaatc tcacgtttca tgtattttct ccataagtaa
    11521 gtat
//
LOCUS       DAVMEF010000055        11402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMEF010000055 DAVMEF010000000
VERSION     DAVMEF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.055"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            154..330
                     /locus_tag="VYQ30_001062"
     CDS             154..330
                     /locus_tag="VYQ30_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966197.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            731..2944
                     /locus_tag="VYQ30_001063"
     CDS             731..2944
                     /locus_tag="VYQ30_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1966198.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMIEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(3166..3642)
                     /locus_tag="VYQ30_001064"
     CDS             complement(3166..3642)
                     /locus_tag="VYQ30_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1966199.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            3865..7068
                     /locus_tag="VYQ30_001065"
     CDS             3865..7068
                     /locus_tag="VYQ30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1966200.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7416..7792
                     /gene="tnpA"
                     /locus_tag="VYQ30_001066"
                     /pseudo
     CDS             7416..7792
                     /gene="tnpA"
                     /locus_tag="VYQ30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8180..8809)
                     /locus_tag="VYQ30_001067"
     CDS             complement(8180..8809)
                     /locus_tag="VYQ30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1966201.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(8819..9820)
                     /locus_tag="VYQ30_001068"
     CDS             complement(8819..9820)
                     /locus_tag="VYQ30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1966202.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(9851..10492)
                     /locus_tag="VYQ30_001069"
     CDS             complement(9851..10492)
                     /locus_tag="VYQ30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1966203.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFDRLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(10511..11326)
                     /locus_tag="VYQ30_001070"
     CDS             complement(10511..11326)
                     /locus_tag="VYQ30_001070"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1966204.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         3612 a         1975 c         2453 g         3362 t
ORIGIN      
        1 caatagtaaa aaagcaaaaa gaatgcgttt tatatttggg tttgaggttt ataaaatagg
       61 ataagcgtgc tgaaaactta gatatgacaa acaaaataaa atttttaaaa gctacataga
      121 aacatggtat aatgtatcta aaggaggtag catatgtcct tagatataga taaagaaaag
      181 atgacaatca tgggaatagc ttttgaaaac agatccgttt ttaagagtgt ttggtatgct
      241 ttaagtacaa atatgattga aggatggcga cctacggtta gtgatgttga aaaattacga
      301 gatgaagctc ttgctctggg gatgacttaa tgaagcgcaa ctgttatgag tcttatgact
      361 atgttgatcc ggataattta tataccgatc cgggttcttc agttctaaga aataagcagg
      421 aaggaaaaaa gcacgcgagc ttgaatatcg tatgattgct agtaagagtt tgaaattatt
      481 tatcaatcct atcttagtta atagtttaaa agtagtttgt ttcacaactg cataagctta
      541 gaaaagttga aaagtgataa ctctttaata aactcttggt ttgttaaaag atattttgtc
      601 cactacattt tttcaattga ctattatttg tcatagatat aacagataga acattgcatt
      661 cagaataatg aaaaacgcat caaaaatggt gcgttctgtt tttatgctac cataaaagga
      721 ggaaaacaaa ttgaaaattt ttaagggaga gttttatcga atctctgtat taacagacaa
      781 gctagtaagg ttagaatact ctcaaactgg aagttttgag gatagaacga cacaacttat
      841 ctataataga gattttggcc aagtttcgtt agattatatc gagacatcaa acgtactaga
      901 tattatgacg gactattttc atctgcactt taataaagga gaatttaacg ccgaaaattt
      961 atttatagaa ttaaaaggaa attttgccgt atatggtagt cgctggtatt ttggtgaatc
     1021 tattgaaacg ttaaaaggaa cagctcggac tctggataag gcagatggag caatctcgtt
     1081 agaagatgga attattagcc gaaatggtat agccttattg gatgattctc aaggatttat
     1141 ttgggatgaa caatctggtt atattgagag agaaaatcaa attgacctgt atttctttgc
     1201 ctatgggcat gattatagag gagcaatcag agacttttac catttgactg gttcaacacc
     1261 cttgttgcca agatatgctt taggtaattg gtggagtaga tattggcctt atacgtcgga
     1321 tgaatacttg gatttaatag acagatttga aacagagaaa attccattat ctatcggtgt
     1381 gttagatatg gattggcata taactgacat tccagctcgt tttggaagtg gctggacagg
     1441 atatagttgg aatagaaact taataccaaa tccagaacag ttattgcaac aacttcatga
     1501 tagaaagtta aaactctcct taaatgtcca tcctgctgat gggatacggg cttatgaaga
     1561 agcttatcct caaatcgcaa aacggttggg gttaaatgta gaactagaag aacctgctat
     1621 ttttgatttt tttaatccct cttttaggga agcctacttt aaagatgttc attatgagct
     1681 agaaaagcag ggagtagatt tttggtggat tgactggcaa caggggacac aaggcatgct
     1741 agatccactt tggcttttaa accattatca ctatcaggat agttgtaaaa atgcagaagg
     1801 tggtttgatt ttatcaagat atgcaggtcc tggtagtcac cgctaccctg ttggtttttc
     1861 aggggatact attattagtt ggaactcctt aagatttcaa ccctatttta cagcgacagc
     1921 atctaatatc ggttatagtt ggtggagtca tgatatcggt ggacatatgt tgggggatta
     1981 tgacgaagag ctacaaacta gatggctaca gtttggtgtt tttagtccga ttactcgatt
     2041 acatagttct agaagtcctt ttaatagtaa agaaccttgg tttttttcag aaacaacatc
     2101 taagattatg aagaaatacc ttcgtttgag acatcagatg attccttatc tatataccat
     2161 gaatgtaaag acacatgagg aaggtgcccc attaatcagt ccaatctatt atttctatcc
     2221 agagaatgat gagagctata atgttccaaa ccaatacttt tttggaacag aactgatggt
     2281 ggctcccatt gtagaaaaga tggatttgac attccaatct gcaaaagtag atgtatggtt
     2341 ccctgaaggt gaatggtatg acttcttttc agagaaaaaa tacacaggtg gtgtgaagtt
     2401 aagtgtttat agagacatct cgactacgcc tgtgtttgca aaaagtggtg caatcattcc
     2461 cttggttggt tctgagatag gtatgggtgt tgatttacct gaagttgtag attggtatgt
     2521 attcccaggc aaacaacatt cttttgaaat gatcgaagat caaaatggtc aaagatataa
     2581 aacaagatta tcaatcgatt gggaaatggg aatggtagag ttagcattac aaggagattc
     2641 tagtatcgtt ccaagcaata gaaaacatag aattcatttt aaaggaacga atgtgtctat
     2701 aattgaattg ccaaataaga atgatacagc taaatttgaa tggaaagata ataaaaggac
     2761 atctttaaat gacgaagttt ttagactact aaagacagct tctcttccat atgaattaaa
     2821 agatagattg ttaaatcaat tcatcaatgc caaaaactct catgacttaa tgaatatctt
     2881 gcatcatcag gataaggaat tgagagggcg tttgttggaa atgatattta ctagccaaaa
     2941 ctaattgaaa taattagaaa gtcatttgtt tagtgtatat gaagattctg tatggtattc
     3001 gttatcactg gtcacattga aaatgtgaaa taagcaccta caacatagac tttacaacac
     3061 ttcatgtttc atatcatggg gtgtttttct atttaagtca aagccaatca aaaaatctca
     3121 ctattcatca ggttaaagga aacctaatca tagtgagatt gtgaattata gtagagtttg
     3181 tatcgcctct tcaatttgtt ttgggtctgt ttttgaagaa aagcgttcaa agacttgccc
     3241 atctcgaccg atgagaaact tagcgaaatt ccattcgatt cgttttccta gtgggccgga
     3301 tttctggtct tttaaccaga catagagagg gtctgcttcc ttaccgttga ccttaatctt
     3361 ggcaaaacgt gggaaggtgg tttgaaaatg taggctacag aaggcgttga tttcctctgc
     3421 gctgccgggt gcttgtccca taaactgatt gcaagggaaa tccaatattt cgaagccctg
     3481 atcttgatag cgttcataga gttcttgaag tccctggtac tggggcgtta aaccacatcc
     3541 agtagcagtg ttgacaatca agagaacttt gccacgatag ctatccaagg gagttgcttg
     3601 gttgttttgg ttcaaaacgg aaaaatcata aagtgaagtc atgagggcct cttttctttt
     3661 tctacattgt aacagttttt acttttttcg tcgagtcgga tggaagatat tgttgaagaa
     3721 taagccctca atatgataga aaccgtttac aaataacttt aaaaatggta tgatttaatt
     3781 aaaccggttt acttagttgc tataaatctc taatttgaga tttagttcct attgatttta
     3841 caatatgttt attggagtgt atacatgcaa acaaaaacaa agaagctcat tgtgagtttg
     3901 tcttcacttg ttttatcagg atttttatta aaccattata tgacagttgg agcggaagaa
     3961 acgactacga atactattca gcaaagccag aaggaagttc agtatcagca aagggataca
     4021 aaaaatttag ttgaaaatgg tgattttggt cagacggagg acggaagcag tccgtggaca
     4081 ggaagcaaag ctcaggggtg gtcagcttgg gtagaccaga agaatagttc tgcagatgcc
     4141 tcaactcgag tcattgaggc taaggatggg gctatcacta tctcaagccc tgagaaatta
     4201 agggcagcgg ttcaccgtat ggttcctatt gaagctaaga aaaagtataa actgcgtttc
     4261 aagattaaaa cagataataa agtcgggatt gccaaagttc gtatcattga ggaaagtggt
     4321 aaggacaagc gattgtggaa ttctgcaacg acgtcaggaa caaaggactg gcagaccatt
     4381 gaagcagact atagcccgac tttagatgtt gataaaatca agctggagtt attctatgaa
     4441 acaggaactg ggactgtttc ctttaaggat attgagctgg tagaggtagc agaccagcct
     4501 tctgaggatt ctcaaacaga taaacagctt gaggaaaaga ttgatttacc aattggaaaa
     4561 aaacatgttt tttctcttgc ggactatact tataaggtag aaaatcctga cgttgcttca
     4621 gtcaaaaatg gaattttaga acctcttaag gaagggacaa ccaatgtcat tgtcagtaaa
     4681 gatggcaagg aagtgaaaaa gattcctttg aagattctag cctctgttaa ggatacatac
     4741 acagaccgtt tggatgactg gaatggcatc atcgctggga atcaatacta tgattctaaa
     4801 aatgaacaga tggccaaatt aaaccaggaa ttggaaggaa aggtagctga tagcctatcc
     4861 agtatttcaa gtcaggcgga ccgcacctat ttgtgggaaa aattttcaaa ttataaaacg
     4921 tctgcaaatc tgactgccac ttatcggaaa ttggaggaga tggccaagca agtgaccaat
     4981 ccttcttctc gttattatca agatgaaact gtcgttcgaa cagtcaggga ttccatggaa
     5041 tggatgcata aacatgtcta caatagtgaa aagagcattg ttgggaactg gtgggattat
     5101 gaaatcggta cacctcgtgc catcaacaat accttgtctc tgatgaaaga atacttctct
     5161 gatgaggaaa ttaaaaaata tacagatgtg attgaaaaat ttgtaccaga tcccgaacat
     5221 ttccgaaaga cgactgataa cccatttaag gctctaggtg gaaacttagt tgatatggga
     5281 agagtaaaag taatagctgg tttactgcgt aaggatgatc aagaaatttc ttctaccatt
     5341 cgctcgattg agcaagtgtt caagttggta gaccaaggtg aaggttttta tcaagatgga
     5401 tcctatatcg accacaccaa tgttgcctat acgggtgctt atgggaatgt tttgattgat
     5461 ggcctgtctc aactgttgcc agtcattcaa aagaccaaga gtccaatcga taaagataaa
     5521 atgcaaacca tgtaccactg gattgataaa tcgtttgctc ctttgctggt gaatggagag
     5581 ctgatggata tgagtcgtgg acgctcgatc agtcgtgcaa atagcgaggg gcacgtggcc
     5641 gcagtagaag tactaagagg gattcaccga atagcggata tgtctgaagg agaaaccaaa
     5701 caacgtttgc agagtcttgt gaagaccatt gttcaatcgg atagttatta tgatgtcttt
     5761 aagaatttga agacttataa ggatatcagt ttgatgcaat ccttgttaag tgatgcagga
     5821 gttgcaagtg ttccaagaac aagttaccta tctgccttta acaagatgga taaaacagcc
     5881 atgtacaatg cagagaaagg gtttggattt ggcttgtcac tcttttccag tcgtaccttg
     5941 aattacgaac acatgaacaa ggaaaataaa cgtggttggt atacgagtga tgggatgttc
     6001 tatctttaca atggcgattt gagtcactat agcgatggct actggccaac agttaatcca
     6061 tataagatgc ctggtacaac agagacggat gctaagagag cggatagcga tacaggtaaa
     6121 gttttaccgt ctgctttcgt tggaacgagc aaactagatg atgccaatgc gacagcaacc
     6181 atggatttca ccaactggaa tcaaacattg actgctcata agagctggtt tatgctaaag
     6241 gataagattg cctttttagg aagcaatatc caaaacactt caacagatac tgctgcaact
     6301 acaattgacc agagaaaact ggaatcaagt aatccatata aagtctatgt caatgataaa
     6361 gaagcctccc ttacagaaca agaaaaggat tatcctgaaa cccaaagtgt ctttttagaa
     6421 tcgtccgatt cgaaaaagaa tattggttac tttttcttta agaagagttc aatcagtatg
     6481 agtaaggctt tgcaaaaggg agcctggaag gatatcaatg aaggacagtc agacaaggaa
     6541 gttgaaaatg aatttcttac gattagtcag gctcataagc aaaatggaga ttcttatggc
     6601 tatatgctca ttcctaacgt ggatcgtgcc accttcaatc aaatgataaa agagttagaa
     6661 agtagcctca tcgaaaataa cgaaaccctt cagtctgttt atgatgccaa acaaggagtt
     6721 tggggcattg tgaaatatga tgattctgtc tctactattt ccaaccaatt ccaagttttg
     6781 aaacgtggag tctatactat tcgaaaagaa ggggatgaat ataagattgc ctactataat
     6841 cctgaaactc aggaatcagc tccagatcag gaagtcttta aaaagctaga gcaagcagct
     6901 cagccacaag tacagaattc aaaagaaaag gaaaaatctg aagaggaaaa gaaccattcg
     6961 gatcaaaaga atctccctca gacaggagaa ggtcagtcaa tcttggcaag tctagggttc
     7021 ttgctacttg gggcgtttta tttattccgt agaggaaaga acaactaatt tgttcatcat
     7081 ctagatgaat tacatgaaac tgtggaaaat agaaaaaatc cgaagaaggc caaatatctt
     7141 cggatttttc ttttaaaaaa gataagttga tacttatctt ttttacttca ttatgtgact
     7201 atagcgtttt gaaactagaa taggacgtca taactgctaa aagatttcta taaattcatt
     7261 tgattttcct aatcaatttg ttcgtatcct atttcactcc actatattag tccgtctcgc
     7321 tccgtgaggt gcgcgtcgaa ggttatacca aaaaaactcc aaacgcgata caataaaggt
     7381 gttcaatcca attgtaaagc gaaaggagaa aaaatatggc acaaaaggct catagtttat
     7441 ctcacacaaa gtggaggtgt tctatcacat tgtgttcacc cctaagtata gacgaaaagt
     7501 catctataat caatatagaa gtagtttagg cgaaatattt catcgcttgt gtagttataa
     7561 aggtgttgaa atgatcgagg gtcacttaat gccagaccat gtacacatgt tagtcagtat
     7621 tccgtcgagg ctaagtgttt caagttttat ggggtattta aaaggcaaaa gtgcactcat
     7681 gatgtttgac aaacacgcca atctcaagta caagtttggg aatcggcatt tctgggcgga
     7741 aggttattat gtaagtccag tagggcttaa tgaagccaca attaagaaat atagtcaaga
     7801 ttaaagaaat tatcgagtgg atgatttctt cacgagtatg aaaatttgag aacgagtaaa
     7861 gcatgatata tagtaaaatg aaataagaac aggacaaaat cgatcaggac agtcaaatcg
     7921 atttctaaca atgttttaga agcagaggtg tactattcca gtttcaatct actataacac
     7981 tagataaatt gagtgtaaaa gaatatgagg atccctttag ggatagtggt aagtaatacc
     8041 aaagtctctt aaagaggcaa gtgacgagtc aagagcaata aggcttgaac aacgtgaaag
     8101 ccagcgtctt taggcgctgg ctgatgattt gggcttatag ctctgagata aaccacccgt
     8161 tagacgggta gttatgattt tatctgagtg taatatactg ttgggcaatc tcgctgatgc
     8221 ggtcaaagtt gccttgggaa gcgagtttat tgagttcgcc accaattcca acggcatctg
     8281 caccagcagc gaaccattga gggatgttgt ttaggccgac tcctccggtt accattacgg
     8341 aaacttgtgg gatcggtgcc ttgactgcag agatatatgc tggactgaga gtactacctg
     8401 ggaagagttt gatgatttca ctaccggctt caagtgcagt cgtgatctct gtgagggtaa
     8461 tacagcctgg aatgtacggt gtgctgtaga gattgcacat tttcgcagtt tcagcatgga
     8521 aagatggaga aacaacgtaa tttgctccag ctagaatggc atctctagca gttacggcat
     8581 caagcacagt acctgcaccg atacaaacac tctgatcgtc ctgatacaag tctacaagtt
     8641 ccttgatgat ttgtcctgca tactgattgg tataggcgat ttcaatagct ttgataccgc
     8701 ccttgataca agcaatcgag gcttgtagtc cttcttcctt tgtatttcct cgaatgacag
     8761 cgacaatttt ctgttttttt agttcaataa tcgtatctga tttggtcatg taattctcct
     8821 aacgaatgat atcttgtgca tttgccagta aattttcaat actagttgcg gaagtggaga
     8881 gatggtctcc tggaagagtg catttgagag ttgcgctcgc aactgcaaag tcaatggtag
     8941 tttttaggga ggaatgatgg agtagttggt aaagggcacc agatataaag gcatccccgc
     9001 taccaattcg ttgatagact gcagttttta gttggacaga ctcttcaaat cgttcttcca
     9061 gagcataggc ttgatagaca tttttgtctt gctcatcact agagcggagg gtatggaaaa
     9121 tggccttgaa accataggct tcttttaaaa gtcgcatgcg attttccacc tcttctaggc
     9181 tagcactgtc tcttggaaac atctctagat tttggtcatc aatcatgaga ggatcaatac
     9241 cgaagcaata gtcagtaaaa cgtgcaaact tagaaaattc atacttggct tctaggactg
     9301 aaatcatctt tgttctcaga ttgagatcca ttgaaacgac aattcctcgg cgcttggctt
     9361 cttccaagag taggagaagg atcgcacgga cctcttgacc gatagctacg gtgattccac
     9421 taaaatgaaa atggctaatc ccctgaaaga gagaatccat atctagcatg tttggccgaa
     9481 tctggctgat actcgtatgc ttacgatcgt agaaaacttc actttgacga caaccaaagc
     9541 cgttctccaa atagtagagg ccgattcgat cgccaagccg acagattgaa ctggtatcga
     9601 tttggtgctg tttcaagaat gtgagaaaac gatctccaat ctcgttggca gggagtgcgg
     9661 taaagacttt cgttgagata cccagggctt gcaaattaca agcgatgtta atttctgatc
     9721 cgccaaaata agtcgaactg gcaacatgat cgccgatact ggtggcatct aatggtgaga
     9781 ttcgaattaa tggttcgcca aacagtaata ttttcgtcat ccattagttc ctttctggtt
     9841 tagaatagct ttacttggct aatacttctt tcaaataagc agcgacttca tcatcttggc
     9901 agttttcaaa gaagtattct tggaaatatt tgccagagat ggtgtctttg aggaagtctt
     9961 ggtcgattgt tttaaggatg gtcatcaaat cattgtgggt gatttgtttc acctcgttta
    10021 agatacgagc gttgcgttgt tcagggatta cacgttctct tgggtagcca ccgcccattt
    10081 cttcagcaaa taagcgatca aacatcaatt tgagggtcag ttctgccccc cagccaaatc
    10141 ctttggcata agggatagac aaggcgttac caccattgat ttgagaataa aggtaagcgt
    10201 cagttgggtc cactgctaga ccacagacaa caccagggaa gctgtttaaa gcaagcatag
    10261 cccctacacc cgtaccacag ccggtaacaa caaagtcaac tgcctttgta tttaaaagga
    10321 tggcagccat tagtccgttc tgcacataag ttaattgact ttctccttct tctccacgca
    10381 taccatagtt aaataattgg tagtcttttt tatctgtcgc ttcttttaga ctatcgtaaa
    10441 taatgtgatt cttgctagct tgactatttt cattgattaa tgcgattttc ataagatctt
    10501 ctttctattt ttactcaggt tgttttccga tgtaggctaa gataccgcca tctacatata
    10561 ggatgtggcc gttgacaaaa ttgctggcat cactagcgag aaagacagca gggcccatca
    10621 aatcttcagg atttccccaa cgtgcagcag gtgtttttgc aatgatgaac tggtcaaatg
    10681 ggtggcgaga accatcttct tgcaactcac gaagaggtgc tgtttgagga gtggcaatat
    10741 aacccggtcc aattccgtta cattggatat tggctccacc gtattcagac gcaatgttgc
    10801 gggtcaacat tttcaagccc cctttagcag cagcataagc gctaactgtt tcacgtccca
    10861 gttcgctcat catcgaacaa atattgataa tttttccatg ccctttcttt atcatagaag
    10921 gaataactgc ctttgaaacg ataaatggtg cgtttaagtc aatatcgatg accttacgga
    10981 aatcagcggc gctcatttcg cacattggaa ctcggcggat aataccagcg ttattaacga
    11041 ggatgtcaat gacaccaacc tcttgttcga tttgcttgac catggcttgg ataccgtcct
    11101 cgtctgtcac gtcacagaca tatccatggg cttcgatgcc aacttcacga taagcttcaa
    11161 tccctttatt gaccagatct tgattgatat cgttaaagac aatagtagcg ccggcttcag
    11221 cgtaggattt ggcaatagca aatccaattc cataagaagc gccggtgatg agagcaattt
    11281 ttccttttaa agaaaactgc tcaattgaga atgatgtatt tgtcatttga tacttccttt
    11341 caaatttgtt aagacttctc tataaccaaa ttatattcta gtaaaccggt tttgtcaata
    11401 aa
//
LOCUS       DAVMEF010000056        10996 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMEF010000056 DAVMEF010000000
VERSION     DAVMEF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.056"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            41..1519
                     /gene="guaB"
                     /locus_tag="VYQ30_001071"
     CDS             41..1519
                     /gene="guaB"
                     /locus_tag="VYQ30_001071"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1966205.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(1570..2667)
                     /gene="recF"
                     /locus_tag="VYQ30_001072"
     CDS             complement(1570..2667)
                     /gene="recF"
                     /locus_tag="VYQ30_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1966206.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTQTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYISGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(2670..3038)
                     /gene="yaaA"
                     /locus_tag="VYQ30_001073"
     CDS             complement(2670..3038)
                     /gene="yaaA"
                     /locus_tag="VYQ30_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1966207.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            3197..4447
                     /locus_tag="VYQ30_001074"
     CDS             3197..4447
                     /locus_tag="VYQ30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1966208.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNTLFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            4444..5727
                     /locus_tag="VYQ30_001075"
     CDS             4444..5727
                     /locus_tag="VYQ30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1966209.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            5769..6590
                     /locus_tag="VYQ30_001076"
     CDS             5769..6590
                     /locus_tag="VYQ30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1966210.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVTLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            6601..7146
                     /gene="pgsA"
                     /locus_tag="VYQ30_001077"
     CDS             6601..7146
                     /gene="pgsA"
                     /locus_tag="VYQ30_001077"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1966211.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            7143..7970
                     /locus_tag="VYQ30_001078"
     CDS             7143..7970
                     /locus_tag="VYQ30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1966212.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            7955..8794
                     /locus_tag="VYQ30_001079"
     CDS             7955..8794
                     /locus_tag="VYQ30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1966213.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            8787..9581
                     /locus_tag="VYQ30_001080"
     CDS             8787..9581
                     /locus_tag="VYQ30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1966214.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRMKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            9640..10458
                     /gene="mreC"
                     /locus_tag="VYQ30_001081"
     CDS             9640..10458
                     /gene="mreC"
                     /locus_tag="VYQ30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1966215.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            10458..10952
                     /gene="mreD"
                     /locus_tag="VYQ30_001082"
     CDS             10458..10952
                     /gene="mreD"
                     /locus_tag="VYQ30_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1966216.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
BASE COUNT         3317 a         1668 c         2342 g         3669 t
ORIGIN      
        1 aagagtcaag ctcaaaaaga aagaaaagag gaaacttcga atgtctaatt gggacactaa
       61 atttttgaaa aaaggtttta cctttgatga tgtattgctt attccagctg aaagtcatgt
      121 gttgcctaac gatgcagatt taacaactaa attggcagat aatttgactt taaatatccc
      181 aattattacc gctgccatgg acacagttac agagagtcaa atggccattg ctattgctcg
      241 tgcaggcggt ctcggagtta tccataaaaa catgtcaatt gctcaacaag cagacgaggt
      301 tcgtaaggta aaacgttctg aaaatggagt tattattgat ccgttcttct tgacgcctga
      361 acatacaatt gctgaagcag atgagcttat gggtcgttac cgcatcagtg gtgttccagt
      421 tgttgaaaca cttgaaaatc gtaaattggt tggtattttg acaaaccgag atcttcgttt
      481 tatttcagat tataatcaac caatttcaaa ccatatgact agtgaaaatc ttgttactgc
      541 tcctgtgggt acggatcttg caacggctga gagtattctt caagagcatc gtattgaaaa
      601 acttccgttg gtcgatgaag aaggcagtct ttctggtttg atcactatca aagatattga
      661 aaaagttatt gagtttccaa atgcggctaa agatgagttt ggtcgtcttc tagttgcagg
      721 tgcagtaggt gttacttcag atacatttga acgtgcagag gctctttttg aggcaggagc
      781 ggatgcgatt gttattgata ctgcacatgg tcattctgca ggtgtcttgc gtaaaattgc
      841 cgagattcgt gctcatttcc cagatcggac tttgattgct ggaaatattg ctactgctga
      901 aggtgcacgt gccctttatg aagcgggtgt agacgttgtt aaggttggta ttggaccagg
      961 ttctatctgt actactcgtg tgattgctgg tgttggtgtt ccgcaagtaa cagctatcta
     1021 cgatgctgca gctgttgcgc gcgaatatgg taaaacgatt attgctgacg gtgggatcaa
     1081 gtattctgga gatattgtaa aagcacttgc tgcaggtgga aatgctgtta tgcttggatc
     1141 tatgtttgct ggaactgatg aagctccagg cgaaactgaa atctttcaag gacgtaaatt
     1201 caagacttac cgtggtatgg gatcaattgc tgctatgaag aaaggttcaa gcgaccgtta
     1261 tttccaaggt tctgtcaatg aagcaaacaa gctcgttcca gaaggaattg aaggtcgtgt
     1321 tgcttataaa ggagcggcag ctgatattgt tttccaaatg attggtggta ttcgctctgg
     1381 tatgggttac tgtggtgcag ctaaccttaa agaactacac gataatgctc aatttattga
     1441 aatgtctggt gctggtttga aagaaagcca tcctcatgat gtgcaaatta ctaatgaggc
     1501 accaaattat tctatgtaaa aaacaatgaa aagaactcca gtgaaaacag gagttctttt
     1561 acaatgttgt caatttccat ttacagcagc cttaccatcc tgaatagtga agatacttag
     1621 attttctggc agattttgaa gatggtctaa gcttgttgtt gtgataaagg tttggattga
     1681 ttgagaaatc gtttctaata attttaactg tctagtgttg tcaagttcac tcatcacatc
     1741 gtcaagcagt aatatcggag attctgtggt aatgctttcc attaattcga tttctgctaa
     1801 ttttatcgag aggacgagac tacgatgttg accttggctt ccgaaactag catccatccc
     1861 acttatataa aaagaaatgt catctcgatg aggaccgaca ccagtattct ttttaaataa
     1921 atctctggat ctactttttt ctaaagcaat tttgaaagat tcggataagt tttgtttgtc
     1981 agttatattg acagaagatt gataggatat tgacaactct tcgatctgat tagagagttc
     2041 aaaatgtttc ttacgcccaa atgattctag tttttttatg aaatctaagc ggtgattcat
     2101 tacacgacat ccataatcaa ctagctgatc atctaacaca gaaaggaatg tttcatctat
     2161 tttttgagct gattttaggt aagtgtttct ttgctttagg atgtggttat aattggttaa
     2221 gtcagataaa tagattggct taatttgccc aagttccata tcaatgaatt ttcgtcgaat
     2281 cgaaggtgct cctttaatta gttgtaaatc ttcaggagca aataagacaa cattcatgtg
     2341 tcctacataa tctgaaaggc gtgcctgttt taagtgatta acttttgtta cacggccttt
     2401 ttgtgttagt tcgatttcta ggggaatgga tccagttttt ttctgaacga gacctgaaag
     2461 atgaagttgt tcctcatcaa aatgaatgag atttttatct gtttgagttc gatgactacg
     2521 cgttaaggct aaaaaataga tagcctctaa catgtttgtt tttccttgtg cattacgtcc
     2581 caaaaagaca ttcaatttag gattaaagtc tattttcgtt tctttgtaat tacgaaaagt
     2641 cttgagagat agatgttgta gccacatgat tatctaccag ggaatcgtgg agcttgttgg
     2701 cttttaggtg atgaagcagg tttcgatttg tcttttttaa ctcccttatt catctttttg
     2761 acaagtttag cgatccgttc tttttcaact ttatcagctt ggtattcatc ttgttcttca
     2821 gaagtaggtt gtgtcaacaa gatgtcaatg ttcatgtcag ggatgtcaac tttatcgcca
     2881 atacgaagtt tttttccacg acgactctct aattccctat taaagtaaac agaatgttca
     2941 gagagaaatg atttaatagc tcctccgcta tgtgtaattc caagttcttt gagtagtgct
     3001 tggagggtaa taaattcttc aaataatttg tattccataa ttcacctcaa tctttggtat
     3061 tataccatat tttcctaaaa atagtgagag agacagcgag aaatgtaagc gatttttatt
     3121 tcaaaagtgt tacagggaac aagaaaattt caggttttcg tgatataata gaagtctgta
     3181 tataaggagg taaatcatgg agttagtgca tggaatttca acacatttta tccaatcaaa
     3241 aaagtttaaa acaaacaaaa ttaccgtgcg ttttaccgct ccattatccc ttgatacgat
     3301 tgcaggtcac atgttgagtg caagtatgct agagactgct aatcagatgt accccacttc
     3361 tcaagatttg aggagacact tggccagtct atacggtaca gatatgtcaa ccaattgttt
     3421 cagaagaggg caaagccaca ttatagaatt gacatttacc tatgttcgtg atgagttttt
     3481 aagtaggaaa aacgtgctaa cctctcagat tttggaactt gtaaaagaaa ctcttttttc
     3541 acccgtagta gttgataatg ggtttgatcc ggccttattt gaaattgaga aaaaacaatt
     3601 gctagcaagt ttagcagctg atatggatga ttctttttat tttgcacata aagaattgga
     3661 taaattgttt tttcatgatg aacgtcttca attggaatat agcgatttac gaaatcgtat
     3721 tttagctgaa actccacaaa gttcttattc ttgtttccaa gaatttttag ccaatgatcg
     3781 aatagatttc tttttcctag gtgattttaa tgaggttgaa attcaaaatg tattagaatc
     3841 atttggcttt aaaggtcgaa aaggagatgt gaaggttcag tattgtcaac cttattctaa
     3901 tatccttcag gaaggtatgg ttcggaaaaa tgtgggacaa tccattttgg aattaggtta
     3961 tcattaccgt tctaaatatg gtgatgagca acatttaccc atgattgtaa tgaatggttt
     4021 acttggtgga tttgctcact ctaagctctt tacaaatgtc cgtgaaaatg ctggattagc
     4081 ttataccatt tcaagtgagc ttgatttatt tagtggattc ttgaggatgt atgctggtat
     4141 caatcgagaa aatcgtaacc aggctcgtaa aatgatgaat aatcaactgc ttgatttaaa
     4201 aaaaggttat tttacagagt ttgagttaaa tcagaccaag gaaatgattc gttggtcgtt
     4261 gttactttct caagataatc aatcttcatt gattgaacgt gcttatcaaa ataccttatt
     4321 tggaaaatct tcagcagact ttaaaagttg gattgcaaag cttgaacaaa ttgacaaaga
     4381 tgctatttgt agagtagcta ataatgtgaa actacaagcg atttacttta tggaaggaat
     4441 agaatgacaa aggttgtttt tgaagaaaaa tactatccag ctgtaaaaga aatggtttat
     4501 cgaactcgtt tggccaacgg attgacagtt gctcttttgc ctaaaaagga atttaaagag
     4561 gtttacggga gtgtaactgt acagtttggt tcgatagata cgcttgtcac agaagttgac
     4621 ggagatgtaa aacaatatcc tggaggaatt gctcattttc ttgaacataa attatttgag
     4681 agagaagatt ctagtgattt gatgtcggct tttacgagtc taggtgcaga tagtaatgcc
     4741 tttacaagct ttacaaaaac aaactatctt ttttcagcaa cggattattt tttagaaaat
     4801 ttatatttac ttgatgaatt ggtaacatca gcacacttta ctgaagcttc cattctgaca
     4861 gagcaggata ttattcagca agaacgagaa atgtaccaag atgatccaga ttcgtgttta
     4921 ttcttttcaa ctttagcgaa tttgtatcct ggtacacctt tagcaactga tatagttgga
     4981 agtgaggagt ccatttccca aatcaatcta actaatttgc aagaaaattt tacaaagttt
     5041 tacaaacctg taaacatgtc tctgttttta gttggtaatt ttgatgtgga gcgagtacag
     5101 gactattttg aaagcaaaga actgaaagat tcagattttc aggaagtagc aagagaaaag
     5161 ttgtttttac agcctgtaaa gccaacagat agtatgagaa tggaagtatc ttctcccaaa
     5221 ctagcgattg gagttagagg taagcgagaa gtttctgaag cggattgcta tcgacatcat
     5281 attttattaa aattattgtt tgcaatgatg tttggttgga cttcggatcg ttttcaaaaa
     5341 tgttatgaat caggtaaaat tgatgcgtcc ttatctctgg aagttgaaat aacaagtcgc
     5401 tttcattttg tcatgttgac aatggatacg aaagagccag ttgctttgtc tcatcaattt
     5461 aggaaggcta ttcgtaattt tacaaaggat ttagatatta cagaggaaca tttagatatt
     5521 atcaaaagag agatgtttgg cgaatttttc agtagcatga actctcttga atttattgca
     5581 acgcaatatg atgcttttga aaatggtgag acaatttttg atttgccgaa aattttacag
     5641 gaaattactt tagaggatgt ccttgatgct ggacatcatt taatagatga tggtgacata
     5701 gttgatttta caatattccc atcgtagtaa cctatcataa tagacactag aaagaaggga
     5761 tgacaagtat gagaaaaaaa acaattggag aggttttacg attagctaga atcaatcagg
     5821 gattgagttt agatgaattg cagaaaaaga cagaaatcca gttagatatg ttggaagcaa
     5881 tggaagcaga cgatttcgat caacttccaa gtccttttta cacgcgttct ttcttgaaaa
     5941 aatatgcatg ggctgttgag ttagatgacc aaattgtttt ggatgcttat gattctggga
     6001 gtatgattac ttatgaggaa gtagatgttg atgaagatga gttgacaggt cgtagacgtt
     6061 caagtaagaa aaagaagaaa aaaacatcat ttttaccttt attttatttt atcctttttg
     6121 ctttatcgat tttaattttt gtgacttatt atgtttggaa ctatattcaa actcaaccag
     6181 aggagccttc tctttctaat tacagtgtgg ttcaatcaac aagttcaact agctctgttc
     6241 cccactcctc aagtagtagt tcttctagta tagaatcagc tataagtgta tcaggcgaag
     6301 gaaatcatgt agaaatcgct tataagacaa gtaaggaaac agttaaattg caattggcag
     6361 tttcagatgt tacaagttgg gtcagtgttt cagaaagcga acttgagggc ggtgtaacct
     6421 tatcgccaaa gaagaaaagt gcagaagcaa cagttgcaac taaaagtcct gtaacaatta
     6481 cgttaggtgt tgtaaaaggt gttactttga cagtagataa tcagactgtt gatttatcga
     6541 aattaacagc tcagactgga caaatcactg taacctttac taaaaattaa ggaaaaacga
     6601 atgaaaaaag aacaaattcc caatctctta acaataggtc gaattctctt tatacctatt
     6661 tttatcttta ttttaacgat aggaaattcg atagagagtc atatagttgc agctattatc
     6721 tttgctgttg ccagtattac cgactattta gatggatatt tagctcgtaa atggaatgtg
     6781 gtcagtaatt ttggtaaatt tgcagatcct atggcggata agttactagt tatgtcggct
     6841 tttattatgt tgattgagtt aggtatggct ccggcttgga ttgttgcagt gattatctgt
     6901 cgtgagttag ctgtgacagg tttaaggctt ttattggttg aaactggtgg aacaatttta
     6961 gcagcagcaa tgcctggaaa aattaaaact tttagtcaga tgtttgctat tattttcttg
     7021 ctattacatt ggactttgct tggtcaagtt ctactttatg tagccttatt tttcactatc
     7081 tactctggct atgactattt taagggtagt gcctatgtat ttaaagggac atttggttcg
     7141 aaatgaaatc aataattgat gtaaaaaatc tttcttttcg ctataaagaa aatcagaact
     7201 actacgatgt gaaggatatt acgtttcacg tgaaacgtgg agaatggctt tcgattgtag
     7261 ggcataatgg tagtggtaaa tcaacgacgg ttcgattaat tgatggctta ctggaagcag
     7321 aatccggaga gattgtaatt gatggccaac gtttgactga ggaaaatgtt tggaatatac
     7381 gtcgtcaaat cggtatggtt tttcaaaatc cagacaatca atttgttgga gcgactgttg
     7441 aagatgatgt tgcttttggt ttggaaaatc agggactttc tcgtcaagaa atgaaaaaga
     7501 gagtggaaga agctctggct ttagttggca tgttggactt taaaaagaga gagccagcgc
     7561 gtctatcagg tggccaaaag caacgtgtgg ccattgcagg tgttgtagcc ctaagaccag
     7621 ctattttaat cttagatgaa gcaacgagta tgttggatcc tgaggggcgt agagaactta
     7681 ttgggacagt aaaaggaatt cgaaaagact atgatatgac agtcatttct attacccatg
     7741 atttggaaga agtcgccatg agtgatcgcg tattggtcat gaaaaaaggg gaaattgaat
     7801 caactagtag tccaagggag cttttctctc gaaatgattt agatcaaatt ggattagacg
     7861 atccttttgc taatcaatta aaaaaatctt tgagccagaa tggctatgat ttacctgaaa
     7921 attatttgac agaaagtgag ctagaggata agctatggga attgctctag aaaatgtgaa
     7981 ttttacatat caagaaggta ctcccttagc ttcagcagct ttgtcggatg tttctttgac
     8041 gattgaagat ggctcttata cagctttaat tgggcacaca ggtagtggta aatcaactat
     8101 tttacaactc ttaaatggtt tattggtgcc aagtcaaggg agtgtgaggg tttttgatac
     8161 cttaatcacc tcgacttcta aaaataaaga tattcgtcaa attagaaaac aggttggctt
     8221 ggtatttcag tttgctgaaa atcagatttt tgaagaaacg gttttgaagg acgttgcttt
     8281 tggaccgcaa aattttggag tttctgaaga agatgctgtg aagactgcgc gtgagaaact
     8341 ggctctggtt ggaattgatg aatcactttt tgatcgtagt ccgtttgagc tgtcaggggg
     8401 acaaatgaga cgtgttgcca ttgcaggcat acttgccatg gagccagcta tattagtctt
     8461 agatgagcca acagctggtc tagatcctct agggagaaaa gagttgatga ccctgttcaa
     8521 aaaactccac cagtcaggga tgaccatcgt cttggtaacg catttgatgg atgatgttgc
     8581 tgaatatgcg aatcaagtct atgtaatgga aaagggacgt ttagtaaagg ggggcaaacc
     8641 aagtgatgtc tttcaagacg ttgtttttat ggaagaagtt cagttgggag tacctaaaat
     8701 tacggccttt tgtaaacgat tggctgatag aggcgtgtca tttaaacgat taccgattaa
     8761 gatagaggag ttcaaggagt cgctaaatgg atagtatgat tttggggcgt tatatcccag
     8821 gggattcgat tgttcaccga ttggatccac gtagcaaatt gcttgctatg atgctactga
     8881 ttttgattgt gttttgggct aataacccct tgacgaatct gattcttttt atagcgacag
     8941 ggatatttat tgccttgtca ggagtatctc tttcattttt tattcagggc ttgaagtcta
     9001 tgtttttctt gattgccttc acaactattt ttcaactatt tttcatttct aatgggaatg
     9061 ttttatttga gttttcgttt gtgagaatca cggattatgc tttgcaacaa gctgggatta
     9121 ttttttgtcg ctttgtattg attattttct tttcaacttt gttaacctta acaaccatgc
     9181 ctttaagttt ggcatcagct gtcgaagctt tattagcacc tttaaagcgt atgaaagttc
     9241 cagttcatga aattggattg atgctgtcta tgagtttgcg ttttgtccca accttgatgg
     9301 atgatacgac tcggattatg aatgcacaga aagctcgtgg agtggatttt ggagaaggaa
     9361 gcatcgttca aaaagtaaag gcgatgattc ccattttgat tcctcttttt gcgacaagtt
     9421 taaaacgtgc agattccttg gctatcgcta tggaagcgcg tggctatcag ggtggaaaag
     9481 gcagaagtca ataccgacaa ttgaaatgga ctctaaagga tacgctgacc attcttgtta
     9541 ttctcgtact tggttgttgt ttatttttct taaaatctta gtagctttca ggaattgata
     9601 aaaagttact gtaacagttt ttgatataaa ggtaggaata tgaaccgttt taaaaaatca
     9661 aaatatgtca ttattgtttt tgtcactgtt ttgcttgtgt cagctctctt agcgacgact
     9721 tattcaagta caattgtgac aaaattagga gatggaatct cattggttga tagagttgta
     9781 caaaaacctt ttcagtggtt tgattctgtc aaatcagatt tggctcattt gacacgaaca
     9841 tataatgaaa atgaaagttt gaagaaacag ctttaccaat tagaagttaa atcaaatgag
     9901 gtggaaagtt taaagacaga aaatgaacaa ctgcgccaat tgcttgatat gaagtctaaa
     9961 ttgcaagcca caaagacttt agcagcagat gttattatgc gttctccggt atcttggaag
    10021 caggagttga ccttagatgc aggtagatca aaaggtgctt ctgagaacat gttagctatt
    10081 gcaaatggtg gcttgattgg gagtgtttca aaagtagagg agaactctac tatagtcaac
    10141 cttctgacaa atacggaaaa tgctgataag atttctgtta aaattcaaca tggctctact
    10201 acaatttatg gaattattat tggctatgac aaggaaaatg acgttcttaa aattagccaa
    10261 ttaaatagta atagcgatat tagtgcggga gataaggtga ctactggtgg attaggaaac
    10321 tttaacgttg ctgatattcc tgttggtgaa gtggttgcca caacgcatag tacagactat
    10381 ttgacacgag aagtaactgt taaattgagt gcagatactc ataatgtaga tgtgatagaa
    10441 ttagtgggga attcataatg agacagttga agcgagttgg agtattttta ttgcttcctt
    10501 tctttgttct aattgacgcc catattagcc agcttctggg ctcatttttc ccccatgtac
    10561 atttggctag tcattttctt tttctatttc tcttatttga gacgatagaa gtatcagagt
    10621 atctctacct agtctattgt tttgttatag gcttggttta tgatgtttac tttttccatc
    10681 taatagggat tacaactctc ttatttatct tattgggagc cttccttcat aaattgaata
    10741 gtgttatttt gttgaatcgt tggacaagaa tgctagctat gattgtgctg acattcctgt
    10801 ttgaaatggg tagttatctt ttggctttta tggtagggtt gacagtagat agcatgtcga
    10861 tttttatagt ctatagcttg gtaccgacga tgattttaaa ttttttatgg attactgttt
    10921 ttcaatttat ttttgaaaaa tattatctat aagaacgaca tataaatgta acaaaggcgt
    10981 aatatttatt aggcct
//
LOCUS       DAVMEF010000057        10959 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMEF010000057 DAVMEF010000000
VERSION     DAVMEF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.057"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            11..733
                     /locus_tag="VYQ30_001083"
     CDS             11..733
                     /locus_tag="VYQ30_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1966217.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VYQ30_001084"
     CDS             730..1578
                     /locus_tag="VYQ30_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1966218.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VYQ30_001085"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VYQ30_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1966219.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VYQ30_001086"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VYQ30_001086"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1966220.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     FSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5957)
                     /locus_tag="VYQ30_001087"
     CDS             complement(3249..5957)
                     /locus_tag="VYQ30_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1966221.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVTWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLININVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5959..6660)
                     /locus_tag="VYQ30_001088"
     CDS             complement(5959..6660)
                     /locus_tag="VYQ30_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966222.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGVTVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6910..9321)
                     /locus_tag="VYQ30_001089"
     CDS             complement(6910..9321)
                     /locus_tag="VYQ30_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1966223.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGKALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TDVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGNGGTGWSKANKINLWARLGDGNRAYKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVFYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9374..10066)
                     /locus_tag="VYQ30_001090"
     CDS             complement(9374..10066)
                     /locus_tag="VYQ30_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1966224.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10601..10941)
                     /locus_tag="VYQ30_001091"
                     /pseudo
     CDS             complement(10601..10941)
                     /locus_tag="VYQ30_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3209 a         2516 c         2040 g         3194 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtt ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgttt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagagct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctatcaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagtgtgact tcattattat gaaaaccgag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattga tcagattgta aaggatgaca
     3601 atagctaata gaaccgacac gatgactaaa atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagccttgcc tttgaaatcc
     4081 ttgtctagcg ttttagaata gattttctgg taggctagta tctcctgccc tttcaatact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagtcacca gataggctgg taacacactc
     4501 gccaaccagc tcaagacaag agctagtaag ctataggtcc aatagaactg aatctgggtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccaaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acggtaaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttgtcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtccttctt ttttcaaaag ttgcagacgt
     5221 accttgccat tatctgataa aatttgttca agctcttctt gatgttgctt ggatttttct
     5281 tcataagctg atgaaaaggc atttaaacct gctaaatctt gatatttcaa acgagctata
     5341 ttgtagactt tctgatcaaa ctgactaggt aaaatcaccc cataggctgt cagagtccca
     5401 cttccacttc ctgcgtagcc catatctcgc tgggagagga tttcagccga atccacaaaa
     5461 ccagtaatgg tataagtatg gtcttttaaa gaggaatgac cctcttcttt ttctttaaaa
     5521 ctaatctcct gtcccacgct gtattggcct tgcaaatgag tggccaaagc gatttccttg
     5581 tctgactgag gaagtcgtcc ctttcttagc tgaaaggttg aaattcgctc tggtttggag
     5641 tacagccgaa tggcatcctg cccattatcc atagtcacat ctgtcaaata gccaaactcg
     5701 acctctgcgc cctccgtctg ttttagttct tcttggtctg cttgatccaa gccatagtta
     5761 gacatgactg ccaaatccaa ggtttgagca gttgttaaat aagcattagc tgtcgcctcc
     5821 atgttgggac tggttacttt gaggcctact aaggctagag atcccaacat catcaggatc
     5881 aagatggata aaaaacgccc cttggagcct gtgaaggact gaattaagtc cttccaataa
     5941 gtttttcgct tgatcatact agtactccaa actgtcaata tcctgaggat gttggttgag
     6001 caccacatcc ttgacactgg catcgtgcat atgaatcacg cgatcagcaa tgggcgccaa
     6061 agctccatta tgagtcacga tgatcaccgt cactcccttt tgacgagaca tatcttggag
     6121 aattttcaaa acctgcttgc ccgtctgata atccaaggct ccagtcggtt catcacaaag
     6181 aagaatttta ggatttttgg ctaccgcgcg tgcaatggag actcgctgtt gctcccctcc
     6241 agaaagctgg gctggaaagt tatttagacg atgagccaga cctacatctg tcaagacctg
     6301 atcaggattc aaggcatctg tcacaatttc agaagccagt tccacatttt ccttagctgt
     6361 cagattagaa actagattat aaaactgaaa aacaaacccc acatcatttc tacggtaatt
     6421 ggtgcgctgg tgggaactat aatccgcaat attaacacca tcaatccaga tttccccttc
     6481 atcattggta tccattcccc caagaaggtt aagaactgtt gacttgcctg cacctgaagc
     6541 accaaggata ataaccagct cccccttttc aatctcaaaa ttcacatcac gattggccac
     6601 aatctccgtg tccccaacct gataacgctt gtaacagtgt ttcatctcaa tataagccat
     6661 cagacccacc ctctatcaaa caaattatac tcctcgaaaa tctctcttca aaccacgtca
     6721 gcttccatct gcaacctcaa aacactgttt tgagcaacct gcggctagtt tcctagtttg
     6781 tgctctttga ttttcattga gtattacttc ctattatcat tataacgctt tttcaactag
     6841 ttgaaaactg cttatattct taaatcctta ttaaaaggca gtttcaaatt actgccttat
     6901 aacatcttct taaaaataaa attgaacaag atcaccttct gctgttgcca ccgaaataca
     6961 atctttcccc atgcatttcg cttttatttt ttcttgattc attttaatca cactcttctc
     7021 aatatctgga taaaaaactc gcaaatctcc tccactcctt gataaaatgg tcaactgtaa
     7081 gagtttttta tcttcccagc tcatgctcac ttcaaaatgt ccacgtgcca ttaagcctga
     7141 aacagaacct gttgaccaag catcaggtag ggcagctaga ggtaccagat aagctgcatg
     7201 agactggagt aacatttctg ccatgccact agtagcacca aaattaccat ctatctgaaa
     7261 aggaggatgg ctacaccaaa gatttggcaa ggtggatgtc tttaactgct ctgccaataa
     7321 tttataggct cgattgccat ctcccaaacg cgcccagaga ttgatcttat tagccttgga
     7381 ccagcctgtg ccgccatttc cacgatcatt gaggctagca cgcgccgctt caatatactc
     7441 ttgtcccttg tagctaaaga gattgccagg atagagtccc actagatggg aagcatgccg
     7501 atgctgggcc tccactttct cattttgaaa atactgctct tcctcctcat accactccct
     7561 gattcgacca gattgagtga tttgcaaagg atttagtaaa tcagacttct ccttaacatc
     7621 agtcaacaag tcctcatcca gtcccaattc ctgagcagcc tgaataaaat catgaaataa
     7681 ctgccaaatc agagattggt cataagtatt gccaatcgaa atcggcccat gttctgggga
     7741 ataagacgga gaagacaccc aacgctgcgc ctgctgatcc ttatgtaaaa aggcattcca
     7801 aaaacgaacc gtttccctca acatgggata aattttctcc ctgagatagt cttggtccct
     7861 ataaaatgaa taggcttcat aaacggtttg catcatccac gcattggcag ctggtgacca
     7921 accccaatag taatcccaac caggtgccgt ccaaccaaag ggagtcgctt gagtatgaac
     7981 caaccaacca ttctcctcac ctttctgaga gacgattcct gcatacttta cagccgctag
     8041 acgaccatag acacgcaaat catctacata gttgatgact ggaaagaccg tctctaggag
     8101 attggtaaca taggctggcc aataattcat ctgcagattg acatttaagt gatagtccga
     8161 attccaagga ggattgtcga ccgcattcca gactccctgt aggttagctg gtagagcatc
     8221 tgggcagtct ctggacgaac taatcaataa ataccgtcca tactggaaga acagctcctc
     8281 caaagccttc ccttcttgtg gcttataatt ttttaacaaa tcatctgtag tggatgcgtc
     8341 aacatcagct tccaaatcca attgaacacg ctggaataaa gcttggtagt cctcgatatg
     8401 ccttgatttc aattgggtat agcccttttc tttagctgtg tccaccaagt ctatcacctg
     8461 ttgctctaaa tctagtttct tgcgataatt gctagcagga ttttgggcaa aatccgtctt
     8521 agcggccaag aagagattgg cataactggc tcctgatatc tgaaccctat ctgaccaaac
     8581 tctaatatct ccatccgttt cccaagctag ataactagca aaccgcagat cattatcctt
     8641 aactcttccc ttcatcaaga tatgagaatc agtaatatcc aacttacact ccttgtaatc
     8701 agatttttcc tgctcatact ttccattaga agccaaatca caggtcaagg atagttctat
     8761 agtaaaatct agagtttcca acccttcctt agtaaaacat tgaaccaaga gatcatctgg
     8821 aaaactcgca aaagcttcac gttcaaatcg cgttccctta tagacataag aagtcgtcgc
     8881 aagtgcctta ctaatattca actgtctctg atagtccgtc acctgagaca aagtcgtacc
     8941 ttgctggctg aactcaatgt gaatatcccc aaaagacaga taggtcccat attgactcgt
     9001 ttttggccca attaggtgct gctcagccag ttccttagcc agattgtaat ctctcttctc
     9061 caaagcctgc cgaatctcag ctaaaaaaac atactgatcc tgaagatttc caccctgata
     9121 atctgaacta tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg
     9181 ttcagcccct ataaggccaa atacttttgc tcctaaagaa ccattaccta tcggtaaagc
     9241 ttcttcttcc caacccatgt aagtggttga agctggttgc ttgtaggcca gtacataatc
     9301 ttgtttttta ttccttatca tacttccata ctaacataaa aagcctagaa atcaatccta
     9361 ggcttacaat tttttatttt ccaaggtagt attcagaaac aacgttcaat ttttcatcga
     9421 attcgaatac caatggtggg aagttaggga tttccacgtc catgatctca tcatctgaca
     9481 aacctttgat gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt
     9541 ttttaccatc tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca
     9601 aagtcacttt caagttttca gcatctggga ttactgagtc gtcaagtgaa gcataacgac
     9661 ggtctgtgtg agctgagtgc tcatcatcac ggtccatatt tggaggcaat acatcgtatg
     9721 aacgacgcca gatgtgaact tgctcatcac caaattgttc agcagcttca gctttgtttt
     9781 taccagtcaa accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc
     9841 ataattggtc agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg
     9901 aagtgtaagc ttggtcaaat tcgataccag cttctttgat caatttacca gcgtcaatcg
     9961 cttgttgtgt acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt
    10021 tgttccattc agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc
    10081 tttaaatttt ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa
    10141 aaagctagtc aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt
    10201 tttggctgtt tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt
    10261 ttgagttgac tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa
    10321 accttcctca gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc
    10381 tgcataagtg cgtattgatt caatcgcatc gtctgttaca accggtatat cgaaagtgat
    10441 tgtttctttt gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt
    10501 ctttgaactt tatgttccac atgcaactag aagagaaaga gataatagag ctactcctat
    10561 accgataaac ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt
    10621 tcttcaatgt tgaaaggaat agagatcctc cgataatagc aagaatcccc ccaacccatc
    10681 ctaagaacgg aatagaatga gactaactcc accagaaaca atcattagta cagacggagc
    10741 tgcacctaca cgcttatctc ctttatagta aacaatagca atgataccaa gaacaagatt
    10801 tgcaatcttc aaggcattca agagtaaggc aacacctgaa aaagcccctg cagtagctcc
    10861 tccattaaga gttgtagcag ctgtgtttac cgctgttccc aacaagacaa aaggaccaat
    10921 aagaagtaaa attcctccca caagacgacg ataccacta
//
LOCUS       DAVMEF010000058        10691 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMEF010000058 DAVMEF010000000
VERSION     DAVMEF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.058"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(73..423)
                     /locus_tag="VYQ30_001092"
     CDS             complement(73..423)
                     /locus_tag="VYQ30_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966225.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(563..898)
                     /locus_tag="VYQ30_001093"
     CDS             complement(563..898)
                     /locus_tag="VYQ30_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966226.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(986..2296)
                     /gene="der"
                     /locus_tag="VYQ30_001094"
     CDS             complement(986..2296)
                     /gene="der"
                     /locus_tag="VYQ30_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1966227.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2310..3023)
                     /locus_tag="VYQ30_001095"
     CDS             complement(2310..3023)
                     /locus_tag="VYQ30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1966228.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3020..3916)
                     /gene="dnaI"
                     /locus_tag="VYQ30_001096"
     CDS             complement(3020..3916)
                     /gene="dnaI"
                     /locus_tag="VYQ30_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1966229.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3917..5086)
                     /locus_tag="VYQ30_001097"
     CDS             complement(3917..5086)
                     /locus_tag="VYQ30_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1966230.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5087..5560)
                     /gene="nrdR"
                     /locus_tag="VYQ30_001098"
     CDS             complement(5087..5560)
                     /gene="nrdR"
                     /locus_tag="VYQ30_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1966231.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5700..6065
                     /locus_tag="VYQ30_001099"
     CDS             5700..6065
                     /locus_tag="VYQ30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1966232.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6070..6765
                     /locus_tag="VYQ30_001100"
     CDS             6070..6765
                     /locus_tag="VYQ30_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966233.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6782..7549
                     /locus_tag="VYQ30_001101"
     CDS             6782..7549
                     /locus_tag="VYQ30_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966234.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7962..9162)
                     /locus_tag="VYQ30_001102"
                     /pseudo
     CDS             complement(7962..9162)
                     /locus_tag="VYQ30_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(9159..10052)
                     /locus_tag="VYQ30_001103"
     CDS             complement(9159..10052)
                     /locus_tag="VYQ30_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966235.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGREFFSILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10169..10249
                     /locus_tag="VYQ30_001104"
                     /pseudo
     CDS             10169..10249
                     /locus_tag="VYQ30_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         3148 a         2351 c         1940 g         3252 t
ORIGIN      
        1 tcaagtttca taatcaaaat aaaaatgact gtatctatag ctccataaac aaatcataga
       61 tacagtcatt ttttagttcc tacaccttcc tttatgcaca cctttagatt tccctagttc
      121 ccgcctcaag cgaatcattt ttttgaaaaa gacataggct aaccaatcac tagcaaaagc
      181 gataagtagt aataatagcc aaatacccca taacttgata tctgtcaaat ttctcaagac
      241 gatattgaaa aacagaactg aaaaaactgt ccaagcaagg ctaaaaagag catagagggt
      301 gatgtaaatc cagtaaaaat agtaaaaaat tgggaaaaat ttactttctc tattatcctt
      361 gtcaatccag ctgaaaaagt aaaaacaggg taataagatt atcattttaa acaagtgttc
      421 catcacaagc ctcctttttt aaattgtttt ctttagtttc gttctattaa atgttattac
      481 caatataaac cttggaatag cgtgtaataa atgattttag aggtaccata agccacctcc
      541 tacaaataga aaccgatata aatcaatgcc ttccaccctt agacttccct agttcctgtc
      601 tcaagcgaaa catttctttg aaacaggaat aagttaacca attcatacca atagctagca
      661 gaataaaaag aaaccaaatg ccccataact tgatatctgt cacatttctc aagacggtat
      721 tgaaaaacag aactgaaaca actgtccaag caaggctaaa aagagaatag aaggggatgt
      781 aaaaccagta aaaataataa aaaattggaa aaaacttact atttctgttg gccttttcaa
      841 tccagttatc aaaataaaag tacggtgcta aaagtaagaa tttaaacaaa tgttccatca
      901 ccgacatccc ccttcttttg atagcgtttt ctattatttt attatatcaa aaaaatccgg
      961 aactgtcatt ccagattcta cttttttatt tgcgttttct tgcgatgaga tgaatcggtg
     1021 ttccctcaaa aacaaaggcc ttgcggattt gattttccaa gaaacgcagg taagaaaagt
     1081 gcatgagttc ttcttcattg acaaagatga caaaggttgg tggtttggtt gccacttggg
     1141 tcgcatagaa aatcttgaga cgttttcctt tgtctgtcgg tgttgggttg atggcaatgg
     1201 catccatgat gacatcgttc aagacagctg atggaatacg tgtattttga ctttcgctga
     1261 tttgcttaat catctcagga agtttgtgga gacgttgctt ggttaaagct gatacaaaga
     1321 taatcggtgc gtaaggcagg tattggaact gctcacggat atcttcttcc cagtttttca
     1381 tagtgtggtt atctttttca agcgtatccc acttgttgac cacgataatc atccctttac
     1441 cagcttcatg agcaaatcca gcgatacgct tgtcgtattc acgaatcccc tcttccgcat
     1501 tgatgaccat taagaccaca tctgaacggt caatagcacg catggcacgc atgacagagt
     1561 acttctcagt attttcataa accttaccag acttacgcat accagccgta tcaatcatgg
     1621 taaactcttg accatctgta tctgtaaagt gggtatcaat agcgtcacga gttgttccag
     1681 caacaggact ggcaatgaca cggtcttctc ccaagatagc attaatcaag cttgattttc
     1741 caacgttagg acgaccaatc aagctaaact taatgacatc tggattttct tcttcatatt
     1801 catttggaag gttttccaca atcgcatcta gtacatcccc tgtaccgata ccatggacag
     1861 atgagatagg cagtggttca cccaaaccga gagcatagaa atcatagata tcatttctca
     1921 tctcagggtt gtccaccttg ttgactgcga ggataactgg tttgtgggtc ttataaagct
     1981 tacgagctac gtattcgtct gcatcagtaa ttccttcctt accagacacg acaaaaacga
     2041 taacatctgc ttcttccatg gcaatttctg cctggtgctt gatttgttcc atgaaaggag
     2101 catcgacatc atcaattcct cctgtatcaa tcatgctaaa agaacgattg agccactcac
     2161 ccgttgcata aatacggtca cgtgtcactc cttcgacatc ttctacaatg gagattcgct
     2221 caccagcgat ccgattaaat agggttgatt tcccaacatt gggacgtcct acaatggcaa
     2281 tagttggtag ggccataatt tctcactttc tacaataatt tcttctgttc aagatttttt
     2341 ctagttgagc ttggttcagc ttgaccaaac tgttctgcta ggcgctgact ccagcttgtg
     2401 gtcgcacgcg ccccagcata gtcagcctga acacggtcat aagcttggat tgcctcagtt
     2461 gactgttctt ggtattcttc ctcaaagaca acattctcta gtggcagtct cggtttcata
     2521 tcatgatgtt gatttggcac acccagtgcc atcccaaaga cagaataggt gtagtcaggt
     2581 aggttaaaga gctctgccac ttcttcagac ttgtatcgaa ccaaaccgat aatcacacca
     2641 ccatagccca agctttcagc tgccaacaag gcgttttgtc cagcaagagc tgcatcgacc
     2701 gaactaatca agagaccttc cacaccttgg ggttggaagg tgtcggtatg aagtctggct
     2761 cccttttctg ctcggttcaa atctccgaca aagagaagga aaacagcaga ctggcgaatg
     2821 gcttcttgag gtaccaattc atacaaggca tctttcttct cttgacttcg taccacaatc
     2881 acagagtagg attggaaatt cttccaagat gatgccatct gggctgctgt caaaatctca
     2941 tttaagtcta cttggggaat ttcttgctct ttaaacctgc gcactgaagt atgagccttc
     3001 atcaatttaa tggtttctgt catcgacggt ttactccttc taaacgagtc tcctcagcca
     3061 aataacggat gcgttccatg acccgtctgg cttcccaggt ttcgtcattt ccatgtttca
     3121 ctttcgcaaa atgcttctcc aaatcttcaa agttgaagtt ggatgtgaaa aaggtcggta
     3181 aattttcctg catccgatat tggagaatga cctgcaggat ttcatcacgc acccaagcgg
     3241 tggattgctc ggcgccaata tcatctaaaa tcaggacctc agacagctta atctcatcca
     3301 ccaaggtctt aacattgcca tcactgatag catttttgac atcaatgaca aagctaggat
     3361 agtggaggag agttgatgaa acaccacgtt tttctgataa atcatgagct aaggccgcca
     3421 ccatgaaact tttacccaca ccaaagtctc catataagta aagacctttt cgaatagctg
     3481 gatattgctc cacgaaggct agtagctttt caaaaactgg taagcgcccc aaatcatcca
     3541 agtcaacttg agccaaacta gctttcttga gactggctgg tagattgatt aacttgagac
     3601 ggttcttaat agccgcttct ttttcagccg cgattagctc aggagtttct tcatatgaaa
     3661 catctgcata gccatgattc ttaaccaaaa tcggcttgta gcctttggca atataatccg
     3721 tatccccacg gagaaacttg tcacgctcgg tgatgtactg attaaacttg gagatactgc
     3781 gatttaattc ctctggagtc aaggattctt gctggataaa agccgcaaca tcagggtcct
     3841 tcatgatttt ctggaccaaa tcttgataat aaaaacggct gggttgacgt ttgagtacgt
     3901 ctccgacact ttccatctaa tctcctcctt tttctaatcg agctaatagt tcttgcttct
     3961 tacgttctag ttccagacga gtttcctcgc tggtttcatt cttatattca ggattactcc
     4021 atttaggaac attggttttt actggggcag tctgattctg tttttgtgtt tttgctttct
     4081 gtcctcgctc acggatgcgc aagactgcct tttctgccga atgaatcttt tgataggcat
     4141 agtcattggc taccttcatg gcatatttct cattgatatt tgccgaatcg accttattaa
     4201 aggtcaataa aagaatgata ttgatgactt cgtccagcaa gcccaagcca gccatctgtt
     4261 gcaagagttc tctttctgtt tgggtaatgg ttcccttgcg tgtttgcttg atttctgcca
     4321 agaactgcag ggcagtttta cttttggctt ctttgataat ggttgtttcc ttaagactaa
     4381 agtcagagga aactggtttt tgagcaattt tttcacgcat gcgtttggtt gaaataacct
     4441 gagaaacagc tgttgacttg gccaattgat aggtttcaaa ccaagtccat ttcttctcct
     4501 cggcaatagc aaagaggttt aagacatcgg actgctcatc cgcaaaacga agtccatctc
     4561 gagccatcag ctggcgaaaa tgttccaagt caaaatcatt ggccactttc ttcttgagac
     4621 caaggtcttc ttgactgcct agttctgcca attctggaaa gacttgattg agtgagacag
     4681 gtatttcttc accatcagca ctttcaactt tcaaatcctc cacagcagag tcgccaatct
     4741 ttttctctaa gagtctgcga taaacaggat gctccaagaa gtcttgacta gatagaggag
     4801 catggagggc tagctgataa acatccccct tttgatagag ggtcaagaga ttaaaagcag
     4861 ataagatttt caatgatttt atcagtctat ccatcccaaa gttgagatga ttgaggatac
     4921 ttgaaaaaag atattccttt ctaccattat cccaaaaact gattgtataa agataaaggc
     4981 tcagtgcctc ctgaccgata atcgggaggt agcactgtac cagagatgag gtatcttgcg
     5041 acacccgatt attctttaga taagaaaaac ggtcaattgg cttcatttat ctttcctttt
     5101 tctttttaga ggactgggtg atttgttgga gcaagctctc taactcactg acatccttaa
     5161 aactacgata gacactagca aaacgtacat aggtaatctc gtccaattca gccaactcct
     5221 ccatgacgag tgaaccaatg tcctcacttt gaatttcatt ttcatttcga ccacggagtt
     5281 tctgttcgat acgattgact accatgttga tttcatcact tgacacagga cgtttctggg
     5341 ctgagcggat aatcccatta aagattttat ctctggagaa ttgttcccgt gtgccatctt
     5401 ttttaacaac cactaaggtt ctttcttcta ctcgttcgta ggttgtaaaa cggtgttggc
     5461 attcgtcgca ctcacgtctt ctacgaatgg tgttcccttc ttctgcttgg cgactatcga
     5521 taacacttga cttggtagcc ccacattttg gacaacgcat cctttccctc cttatcgttt
     5581 tcttttcatt ataccatttt ttaagcgatt cccaaaacaa ttcttctttt tgcttgacaa
     5641 gttttttgtt ttgttgtatt atttaattaa gacaaaaggg taagagaaag gagaccaaga
     5701 tgtcctggac atttgacaac aaaaaaccca tctatttaca gattatggaa aaaatcaagc
     5761 ttcagattgt ttcccataca ctggaaccca atcaacaact tccaaccgtg agggagctag
     5821 ctagcgaggc tggtgtcaat cccaatacca tccaaagagc cttatcagac cttgaacgag
     5881 aaggatttgt ctacagcaag cgaacaactg gacgatttgt gactaaggat aaggagctaa
     5941 tcgcccagtc acgcaaacaa ttatcagaag aagaattgga acacttcgtt tcctccatga
     6001 cccattttgg ctatgaaaaa gaagaactac caggcgtagt cagtgattat attaaaggag
     6061 tttaagccta tgtcattact agcatttgaa aatgtatcca aatcatatgg agcaacacca
     6121 gcccttgaaa atgtttctct tgacattcca gctggaaaaa ttgtcggcct tcttgggcca
     6181 aacggctcag gaaaaacaac cctgattaaa ctaattaatg gcctcttaca accagatcaa
     6241 ggacgtgtcc tcatcaacga catggaccca agcccagcaa ccaaggccgt tgtagcttat
     6301 ttgcctgata cgacctatct caatgagcaa atgaaggtca aagaagccct aacctacttc
     6361 aagaccttct ataaagattt caatcttgaa cgcgcccatc atctacttgc agacctgggc
     6421 attgatgaaa atagtcgtct caagaaacta tcaaaaggaa acaaagaaaa ggttcaactg
     6481 attttggtta tgagccgtga tgctcgtctc tatgttttgg atgaacccat tggtggggtg
     6541 gatccagcag cccgtgctta tatcctcaat accattatca acaactactc accaacttct
     6601 accgttttga tttctaccca cttgatttct gatatcgagc caatcttgga tgaaattgtc
     6661 ttcctaaaag acggaaaagt cgtccgtcaa ggaaatgtag atgatattcg ctacgagtca
     6721 ggtgaatcca ttgaccaact cttccgtcag gaatttaagg cctaagcaaa ggagattatt
     6781 tatgttttgg aatttagttc actacgaatt taaaaatgtt aacaagtggt atttagccct
     6841 ctacgcagcc gtgctagtcc tttctgccct catcggaata cagacacaag gctttaaaaa
     6901 tctaccttac caagaaagtc aggctactat gctacttttt ctagctacag tctttggtgg
     6961 cttgatgctt acacttgcga tttcaaccat tttcttgatt attaaacgct tcaaaggtag
     7021 tgtctacgac cgacaaggct atctgacttt gaccttgcca gtttctgaac accatatcat
     7081 cacagccaaa ctaatcggtg cctttatctg gtcattgatt agcaccgctg tattggctct
     7141 aagtgctgtt attattctgg ctttaacagc tccagaatgg attcctcttt cttatgtgat
     7201 tacatttgta gaaacacatc tccctcagat ctttcttaca ggtatatcct tcctactaaa
     7261 taccatttca ggaatcctct gcatctacct ggctatttcc attggacagc ttttcaatga
     7321 ataccgtaca gcactcgctg ttgcagtcta cattggtatc caaatcgtca ttggatttat
     7381 tgaacttttc ttcaatctta gttctaattt ctatgtcaat tcactggtag gactcaatga
     7441 ccatttctat atgggagcag gtatagccat tgttgaagaa ctcatattca tagctatctt
     7501 ttatctcgga acctactaca tcttgagaaa taaggttaat ttgctttaaa taatttttac
     7561 ctagatatgt aacatactca tagaacaaaa gagaccaggc aaaaagtctt taaaattaga
     7621 aaacgcatag tatcaagtgt tgaatatgaa ctgcccccca aaagttagac agaaaaaatc
     7681 taacttttgg ggggcagttc aaaaaatcct tgatattatg cgttttttgt gagctgactt
     7741 atttcctttc actatatcgc aaaatgaaat aagaacggaa cgatgggatt ttggaattca
     7801 aatcaatttg taagaatgtt ttagaagtaa tattatccta ttccagattc agttcactat
     7861 acaattgagt tttcaagcaa cttgtttaca taatgtgtac ataattaggt tcgtgattcc
     7921 acccttttca cctttaaaaa cctcgctttc gcaaggctct tctatttata agataaggca
     7981 cgtttaaagg ttttccaaat ccctaaatca tccgtttgaa gaacgagact agcatacatg
     8041 cgtccgataa atcctgttgc taccaccgca aaaatcactg taatagcaag tgaaatccat
     8101 gcttctgctc ccccccgcat agtcattaat cgttcgaaac ggcataaaga aggtcgaaat
     8161 aaagggaata taagaaccaa tcttcaagag gagattgtca ccagctgcac ctagagctgt
     8221 cactccaaaa aaaccaccca taatcaaaat catcaaaggc gacaaggctt tccctgagtc
     8281 ctcaggacga gaaaccatag atcctaggaa ggctgccaag actacgtaca tgaaaagact
     8341 gatcaaaata aagagcaagg tattcagtga gatagcatct cccaagtgat ccaaaatacc
     8401 agactgagcc aagaatggca aatctttaaa gagcaaaacg gcagccagac cacctacaac
     8461 atagatccca atatgcgtta aaatcactag aaacagagcc atcatccgcg catagaaata
     8521 gtgacttgcc cttatgctag aaaaaacgac ttccataatt ttggtgcctt tttcactggc
     8581 aacttcctga gctgttacac ccgcataggt aatcagaatc atataaagaa agaatcctaa
     8641 ggcacctgct gcaattgttt gaataaactt tttattttcc ttggcttcat caatcttttc
     8701 tgtgaattga attgtctgcg ctaagcgttt ttcctgctct tgagacaagg aagcagttga
     8761 acgattaagc tgattttgca gttcattgag tgtacctgta acctcaaatt taattccatt
     8821 ttcaagcgat gtttcgccat gataaactgc ctttagaaca ctatcttctt gatcaatggt
     8881 caaataacct tttaattttt cttctttaat tgcttctttg gcacttgctt cgtctttata
     8941 gtcgaagtta acaccattta cattcttcag tccttctgct acagatggca ctgttgtcac
     9001 tactgccact ttattatttt tagccataga agaaccttgg agatgcccaa ttcctacaga
     9061 gattcctaaa aagaggaacg gcgaaatcac cataaagaag aaactccatg actcgacatg
     9121 tcgaagatag gtttccttga ttacaaccca catatttctc atacttccac tcctgattct
     9181 agtttaaaga tttcatcgat agttggcgct tgttggtcaa atgttgcgat atattgacct
     9241 tgagtcaaga ttgagaagaa ttcccttcca gcgctctcat cctccaaaat caatttccaa
     9301 ctgccttgtt tggtcaagct cacctgtttg acatgaggaa gattttccaa ttcttccttg
     9361 cttcgttcac ttgaaacaaa gagacgcgtt ttcccgtatt gattgcggac atcctgaact
     9421 ggtccgtgca agaccacacg gccatctcgg atcatcagaa tatcgtcaca aagttcctca
     9481 acattggtca tgacatggtc agaaaagata atggttgctc cgcgctcttt ttcctgaaaa
     9541 atgacttgtt tgagcaattc tgtattaact gggtccaatc cactaaaagg ctcatccaag
     9601 ataatcaggt ctggttcatg aatcagagta ataatgagct gaatcttctg ctgatttcct
     9661 tttgacagac tcttgatttt atctgtcagc tttcctttca cttccaacct cttcatccat
     9721 tgagggagtt tttctttgac ttctttggca tccatgcctt ttagagtcgc caagtagcga
     9781 acttgttcaa gaactgtcaa tttaggcatg agactgcgtt cttcaggcag ataaccaatc
     9841 cgagcatagg tctcctgacg aatatcctga ccatccagac cgatttctcc ctgatattct
     9901 aggaatttca aaatactatg gaaaatcgtt gtttttccag caccattttt tccgactagt
     9961 cccaaaatac gacctggtcg cgcttgaaag tcaataccaa acaaaacttg cttggatcca
    10021 aaacttttct ctagacttct tacttctagc atctttcacc tccgaaattt cttgcactca
    10081 ttatactcct ttttgatagc ctttacaatg ttttttgtcc atttttagaa gactattgct
    10141 gtgtaaaata tggcctggag cacttttata ctcaatgaaa atcaaagagc aaactaggaa
    10201 gctagccgta gactgctcaa agtacagctt tgaggttgca gataaaactg acgaagtcag
    10261 ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaact atatctacgg
    10321 caaggcgaag ctgacgtggt ttgaagagat tttcgaagag tattagtgat aaatccatta
    10381 tacagcagca aacttaattt ataccttccg ctcctcaact gtctattttt aatcctgaat
    10441 tgttatttga gtaactcctt tttcctcata aagttttctt cctctaaaac ttctggaaaa
    10501 aagcgaatag tttcagacaa catttttata agaaacaagt tcatctgtca tttcaagaag
    10561 gagtaatcct ttatctacta atggacgaaa cggaattcaa ccgcttgtcc gatatgtttt
    10621 ctaaggatta tatagcaaaa tgaaataaaa acaggacaaa tagatcggga cagtcaaatc
    10681 gatttctaac a
//
LOCUS       DAVMEF010000059        10656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMEF010000059 DAVMEF010000000
VERSION     DAVMEF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.059"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(240..1682)
                     /gene="gtfA"
                     /locus_tag="VYQ30_001105"
     CDS             complement(240..1682)
                     /gene="gtfA"
                     /locus_tag="VYQ30_001105"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1966236.1"
                     /translation="MPIQNKIMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(2002..2835)
                     /locus_tag="VYQ30_001106"
     CDS             complement(2002..2835)
                     /locus_tag="VYQ30_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1966237.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFSIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            complement(2849..3715)
                     /locus_tag="VYQ30_001107"
     CDS             complement(2849..3715)
                     /locus_tag="VYQ30_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1966238.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(3729..5004)
                     /locus_tag="VYQ30_001108"
                     /pseudo
     CDS             complement(3729..5004)
                     /locus_tag="VYQ30_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(5039..7201)
                     /locus_tag="VYQ30_001109"
     CDS             complement(5039..7201)
                     /locus_tag="VYQ30_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1966239.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKNLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            7309..8169
                     /locus_tag="VYQ30_001110"
     CDS             7309..8169
                     /locus_tag="VYQ30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1966240.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            8166..9101
                     /locus_tag="VYQ30_001111"
     CDS             8166..9101
                     /locus_tag="VYQ30_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1966241.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(9227..9314)
                     /locus_tag="VYQ30_001112"
     tRNA            complement(9227..9314)
                     /locus_tag="VYQ30_001112"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9278..9280),aa:Ser,seq:gct)
     gene            complement(9321..9394)
                     /locus_tag="VYQ30_001113"
     tRNA            complement(9321..9394)
                     /locus_tag="VYQ30_001113"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9358..9360),aa:Ile,seq:gat)
     gene            complement(9430..9500)
                     /locus_tag="VYQ30_001114"
     tRNA            complement(9430..9500)
                     /locus_tag="VYQ30_001114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9466..9468),aa:Gly,seq:tcc)
     gene            complement(9520..9592)
                     /locus_tag="VYQ30_001115"
     tRNA            complement(9520..9592)
                     /locus_tag="VYQ30_001115"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9557..9559),aa:Phe,seq:gaa)
     gene            complement(9596..9669)
                     /locus_tag="VYQ30_001116"
     tRNA            complement(9596..9669)
                     /locus_tag="VYQ30_001116"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9633..9635),aa:Met,seq:cat)
     gene            complement(9683..9772)
                     /locus_tag="VYQ30_001117"
     tRNA            complement(9683..9772)
                     /locus_tag="VYQ30_001117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9734..9736),aa:Ser,seq:tga)
     gene            complement(9783..9856)
                     /locus_tag="VYQ30_001118"
     tRNA            complement(9783..9856)
                     /locus_tag="VYQ30_001118"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9820..9822),aa:Ile,seq:cat)
     gene            complement(9870..9943)
                     /locus_tag="VYQ30_001119"
     tRNA            complement(9870..9943)
                     /locus_tag="VYQ30_001119"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9907..9909),aa:Met,seq:cat)
     gene            complement(9949..10022)
                     /locus_tag="VYQ30_001120"
     tRNA            complement(9949..10022)
                     /locus_tag="VYQ30_001120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9986..9988),aa:Pro,seq:tgg)
     gene            complement(10038..10111)
                     /locus_tag="VYQ30_001121"
     tRNA            complement(10038..10111)
                     /locus_tag="VYQ30_001121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10075..10077),aa:Arg,seq:acg)
     gene            complement(10121..10206)
                     /locus_tag="VYQ30_001122"
     tRNA            complement(10121..10206)
                     /locus_tag="VYQ30_001122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10170..10172),aa:Leu,seq:taa)
     gene            complement(10214..10285)
                     /locus_tag="VYQ30_001123"
     tRNA            complement(10214..10285)
                     /locus_tag="VYQ30_001123"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10251..10253),aa:Gly,seq:gcc)
     gene            complement(10308..10380)
                     /locus_tag="VYQ30_001124"
     tRNA            complement(10308..10380)
                     /locus_tag="VYQ30_001124"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10345..10347),aa:Thr,seq:tgt)
     gene            complement(10399..10480)
                     /locus_tag="VYQ30_001125"
     tRNA            complement(10399..10480)
                     /locus_tag="VYQ30_001125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10444..10446),aa:Leu,seq:tag)
     gene            complement(10486..10558)
                     /locus_tag="VYQ30_001126"
     tRNA            complement(10486..10558)
                     /locus_tag="VYQ30_001126"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10523..10525),aa:Lys,seq:ttt)
     gene            complement(10583..10655)
                     /locus_tag="VYQ30_001127"
     tRNA            complement(10583..10655)
                     /locus_tag="VYQ30_001127"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10620..10622),aa:Asp,seq:gtc)
BASE COUNT         3300 a         2357 c         1923 g         3076 t
ORIGIN      
        1 aaaaagataa aagggtaaaa agatactcag gataattccc aataaaatca gctctttact
       61 agacaacccc ttcagctttt caataaagcc tattgatttc aaaatgaatc ctttctctcc
      121 aaatcagctg attcagataa tagtaagcta tcctatattg taccactttt ttagcaattt
      181 gaaaacaaag gaaacgtttt ccaaaataaa aaccctattt tatccaccat atcaaggctt
      241 caaaatgata cttcaactcc attctcaatt acccgataag tctgattttg caaatcaatt
      301 tctactactg ctgttacgga cttatcttta ttttgacgtt tgattacaat gctgtgagct
      361 gttggtgtct ctatctcagt agtcccttct agatcaaagg cttctgaacg gttacggaaa
      421 gaaaatagat tgagaagggc cttcacaaca ggtcgttgca cttcttttgc tatttcctcg
      481 ttgctatagt aatgacgatt aatatttcga ccttctttag tttcttctaa taatttcaag
      541 tcattcttgc ctgctaatag acccacatag taaacctgag gaatacctgg ggcaaaagct
      601 tgaattagac gagcgagaaa atacttgaca tcatcatctc caagcgctga atagtaggtt
      661 gaattgattt ggtagatatc taagttgtta tactcggcac tagagtactt acgtttgaca
      721 ttggctccaa ccttatagag ttcatttgaa gcatagtcaa tctcctcatc ggtcaggata
      781 tccttgacat ctactactcc aatcccatca tgggtatcta gcgtcgtaaa ttgcttcatc
      841 gggctcatct ttaaccactt agccaaacgc tctgttctgg aactgtaaag agtataaagt
      901 gtcaccattg gaagagcaaa atcataaaca tagtaatcat ggtctgctat tttaaactga
      961 atcgaatagt gttcatgaat ctcaggtaaa agctctgtcc catactcagc agcgatatct
     1021 cgaactttgt ccaataaatc ccaaatatct ggttccacaa agaaatcatt agtatccaat
     1081 ttcttcactg cataagcaaa ggcatctaga cgaatcaaat cacacccatt acttgccaag
     1141 tgctgaatgg tcttacggat aaattccata gttacttctt tggtcacatc aagatcaatc
     1201 tgctcctcac caaaggtatt ccacaaatgt tccactgaac catcttcaaa cacaatctct
     1261 tgctttggtg cacgatcctt acgcttgtaa attaaatcta catcagactg tgtcggacgg
     1321 ttttctggcc aaaacttatc ccagtttaaa aagagatctt taaattcact ggcttcatgt
     1381 ttttcttgat agtccttata atacttggat tgacgagaaa tatgattaat cataaaatca
     1441 aacataagat aatatttctc acctaaacgc ttaacatcct cccaatcacc aaaagctgag
     1501 tccacttcgt cgtagtcaac tggcgcaaat ccacgatcac ctgttgatgg gaaaaatggt
     1561 aaaaggtgaa ctcctccaat agcatctcca aaatgctctt ccaaattatc atataagtct
     1621 ttaagattat ttccaaggct atcagaatag gtaatcaaca tgattttatt ttgaattggc
     1681 atcattactc tcctttttct aattgaagcc aagtctcata tgatctggct tcgtaaatca
     1741 aattcatttt atactcttcg aaaatctctt caaaccacgt cagttctatc tgcaacctca
     1801 aaacagtgtt ttgagcagcc tgcggctagc ttcctagttt gctctttgat tttcattgag
     1861 tattaaatct ctatttatca tcaaactcgt actaaatata gacttcaata aactaagtac
     1921 tactttcttg ttttcacata aaattgtcaa ctagctgaac tcttcctctg tgtcagagac
     1981 tatagattct atgagctctt cttacttcac tgccccgttg cttattcctg cgatgatatg
     2041 gcgttggaag aagagataga caatggtgat actgataatg ccgaccacgt aagaggcaaa
     2101 gcttggtccg tagtcgttga aatattggcc tgcgtagttg tattggaaca aaggcagagt
     2161 ccacattttg gaatcccggt tcaagacaag aagtggcaac atgaagtcat tccagaacca
     2221 aagggcattg atgatcatgg ttgtcgcatg catcggtttc atcattggga agatgatgct
     2281 gaaataggtt gtaaattgat tagccccatc gatctctgct gcttcatcca gactttctgg
     2341 aatcgagatt ttgatatagc caacatagag aaagagggtc tgtggaatcg cataggtcaa
     2401 gtagagcaag atcaaaccaa aggtattagc caaaccgagt ttactcatca taaccgtaat
     2461 cggaatcatg atgacttgga aaggtacgaa tattccgagg attaagaggg tatacatgat
     2521 ggtaaaggct tttcttttac tcatattgcg agcgatggag taggctgcca tagggataaa
     2581 gatcattact gcaagtaaag acaagacagt gatgacgaca gagttccaat aatagcctcc
     2641 aatcccatca gctaagagac ggctaaagtt gtcccatgtg aagttggttg gaaagccaaa
     2701 gaaattatct acaatatcct tagtgggttt gaaagaacta aagagggtag caaggagcgg
     2761 cactaaaatc agaaccgatc ctagaatcaa tagaatgtat ttgccaatca gggcttttct
     2821 ttcatcttgt ttcatcatgc ttctcctctt aaatttcaaa tttcttagat actctcaatt
     2881 ggatgatcga aatcactaca attaagaaga acaagattac ggcaatggca ttggcataac
     2941 cgaattggtt gtttttaaag gcatagttat aaaccaagag cccaagtgag gttgtggcat
     3001 tgtttggacc accaccggtc atggcaaaga cttggtcaaa ggcagtcagc ccacctttta
     3061 gggctaggat aaagactata gagacacttg gtagcaagta aggcaattca atgttccaga
     3121 aaacttgctt gctagtcgca ccatcaatcc ttgctgcctc tgtaatctca gttggaatag
     3181 attgcaaacc agctaggaag atgatgatgg gcatagccac cccttgccaa agaaggacaa
     3241 agacagccgc aaagattgct ccccacttag tccctaaaag actggtttgg aaaaattcaa
     3301 tatgaagggc attcccaatc gctggaagac cgtagttgaa gacttgcttg aagatcaaag
     3361 ccactgtcaa accagataaa acagctggga agaagaacca agcacggaag aaggtttggc
     3421 ctttgatttt agagttcaag acacgcgcaa tgaagatccc gagtgcaatc tcaccaacca
     3481 ccatggcaat cgtaatgatt gcggtaaagc caatcgcatt catgaatttt ggatccatga
     3541 agaggagctt aaagttgttt aagccgacaa atttgtagtt ataagtcaat cctgtccagt
     3601 tggtaaaact gtaaaaggct ccttgaaaca tcggcacata gaagaaaatt gcttgtaaca
     3661 agagggggat gaccacaaaa gcccatgccc aatatttttg taatactttt ttcatagtct
     3721 ctctactcct aatccacatc cgctttcatc gggttaaaga aggcattcaa atcattgacc
     3781 atgccttgtt tatcaccggt caagacatag ttcatggtca aggtatggaa gtctgcttca
     3841 ctggtccaat attgttgcaa ccagaccaag tgacgatccg taaaggcata ttcggtcata
     3901 ccagcaagcg gtgaatcttc tcctgcttgt ttgacccctt cgatcgctgt tggagatccg
     3961 tccacatcgt agtatttttg catgacttct ggacgggtca tatattccac aaaggcattg
     4021 gcttcttttg gatgtttggt ggtggctgag atagaccatg ccaagtctcc cgcaccaacg
     4081 gttaagcttt gtcctttttc ttttcctgga atcatgaagg tcccaatctt aaagttcggt
     4141 ttttgttcat taatcgctgt gatcgcccaa gacccatttg gtgtcatgag gacatcccca
     4201 cgtgcgaagg ctccgataac atcggtatag ccagcacctt cccagttctt ttgcttagag
     4261 ccattgatgc gaaggatgtc catgaccttg atatcatctt tcataatcgg atccgacaat
     4321 ttaatggcat ttggttgaga ataacgaagg tattgatttg cttcttttcc tccacctgtt
     4381 gctgtcgcaa aggctaattg attgtaacca ttgagtgtcc aagcatctgc acctgcaatt
     4441 ccaaatggtg tttgtccttt agcaacgata tctttgacta actgttcaaa ttcatcccag
     4501 gtttcaggaa ccttcaagcc cagttcttcg aatttatctt tgttgtagta aattccataa
     4561 gcattagctg taaaaggaac gttgtaaact ttttcgttta cagcatattt ttcagcgtag
     4621 ccatttttca gcgtagccat ttttcacgcg tttcaggtag tctttgttgc tcaaatcttc
     4681 aaagacacct gcttttgccc attcttgcag ttcgatggac tgtgggtaaa tattgaccac
     4741 atcaggcaca tctcctgcga gaacgcgtgt cttcaatact tcaccagcat ttggtacatt
     4801 gacgactttg accttgatct tagggttttc cttctcaaaa tcacgagtga tttcttccaa
     4861 ggttttggtc atttcttttt tctggttgaa atactcgatg gtcactgtgc catccgcaga
     4921 tttaccatag ttggagcaag cgccgagccc aaccaaagct aaacctgtag ttgcaagaag
     4981 tccgattttt ttataccatt ccattagaaa gcctccttta taaatttata cacccttatc
     5041 atagtcttct aaaaatatac tgtctactca aaaaatctcc ttgggataag ataacagtta
     5101 ggcccgcata cattagttct gcacctgagt aaacttcgcc attttcctgt aatttatata
     5161 gtccctcttc atccaaattt tttaatttta aagttgtttc catggtctct acaacagata
     5221 aaacgcgaac gtaggttaca atcgtttgat ttccgtaatt aaattgtaca gctgcttcat
     5281 tggatacagt atcaggatta attagtctat actgctgtcc taactgaact actggtcgta
     5341 attctttata caagttcacc tgattagcaa tcgtagcttt ctcttcatct gataaatttg
     5401 tcaaatcaag ttcatagccc aaatttccca tcattgctac aaggccacgt gtttctaatg
     5461 gtgtcattcg tcccatctga tgattcggta ctgctgacac atgagccccc atagaaatgg
     5521 ttggatagag ataggatgaa ccgtattgaa ttggtaaacg tgcaatggca tcagtattat
     5581 cactagccca gacttgtggg aaatagcgca tcataccaag atcatttcgt ccaccaccac
     5641 cagagcagga ctcaaagaga atatggctgt gcttctctgt cagataagaa acgagttcgt
     5701 aaagccccag catgtactga tgagattgca tctgtgtctc tagataagtt aatccattcc
     5761 ctagcttagt gatattgcgg ttcatatccc atttaatgta atcaatatca tgataagata
     5821 ggagttgatc taagacactt ttcaagtatt ctactacctg aggattggca agattaagta
     5881 ctaattgatt ccgagaataa gtatgctcat agtcaggaac ctgaatagcc cagtcaggat
     5941 gtcgacgata caaatcacta tctacagaaa tcatttcggg ttctaaccaa agtccaaact
     6001 gcaaacctct ttcatggata gctgaaatca gactttctag acttccaccc agtttttcct
     6061 cattaacaac ccaatcacct aaagcacgat tatcatcaaa acgattgcca aaccaaccat
     6121 catctaatac aaaaagttca atgccaactt tcttagcttc atctgctaac tctaacagtt
     6181 tttctctctg aaagtcaaag taagtagctt cccagttatt gattagaatt ggacgttctt
     6241 ttttagaaaa ttcacttagc ataatgtgct tcagtacaaa attctgactt tcatgactaa
     6301 taccagttaa tccctgatct gaatgggtca ctaaagctac cggtgtttca aagtattcct
     6361 caggagctaa cttccaagaa aagttttctg gattaatgcc aatagccacc cgaacttcat
     6421 tcaattgatt tttttgaaca aaagcttcaa agttgccact atacattagt tgaatagcaa
     6481 acacattccc agcatcctct gtgactcctt gttcgcatag tagaagagct ggtgtttgag
     6541 catgaccaga agcacctcgg tttgagctaa tcgaaaagat tccttgttct acctgttgac
     6601 gtctaacagt cttttcacga gcataagcac cctgcagagt tactatttcg taatctgcag
     6661 ctggaaaatc agccataaaa gaaaaatctt tatggatgac aacttcctga ttactattat
     6721 tatctaattt actgtagcta gcaatagtcg catcattatt aaaagtagta taatacaaag
     6781 tcagactaag ttgagcctta gaatcttcta acattaagac aagagtctct gtatcgtcca
     6841 tgctatgtgg agaaggtaag ccctgtggac cattctgacc ttttaaaatc tttgcttcta
     6901 caaatcgaaa gtctgttact tcagttacac tatgctgaac ctgtatggtt ggtttcctaa
     6961 aatctcctaa gccatgttgt ccaaaaatct gtcgctgagt atctaaacta aaggttcgat
     7021 tagtagccgt tggatttcct gaaaaggcat ggtctcgttc ataaacacta ttggaacctt
     7081 tatagttctt aatagtcttt cctaaatgtt tcaaaagtaa gtagccattt cgattttcaa
     7141 taatcaaact tagattttta ctctcaacat aaaatagatt attctctatc ctaactccca
     7201 tttacttcac ctcatcactt tattgattat attttatcac ctgaaatcgc tttccaaaat
     7261 agaaaaatgt ctcaagaata tggtaaaatg ttaggtagga ggtagcacat gttagttttt
     7321 tcagaatacc agactggaac aatcgacctt gccctaagct tttatggata tgaggaatgc
     7381 acacctaatt actcttttgg tccagccatt cgtgatacat acgttctaca ttacattact
     7441 aaaggacaag gaaaatttta ttacaagggt aaaattgttg atttaaaaga aggagatttc
     7501 tttctattaa aaccagagga actaaccttt tatcaagcag atagtaaaga accttgggcc
     7561 tactactggt taggaatcac tggagggaaa tcccctgatt attttgctct ttctcaaatt
     7621 tctgatcaat cctatctcat ccaatctgaa acttgtcata cccagactac tgcaaaactc
     7681 atctcagaca ttgtccgctt cgctcagatt acaaaatcaa gtgaattagc tcaactccat
     7741 atcatgggac aacttcatga actgatgttt catctgggaa ctattgctcc caatcagaaa
     7801 aaaaagaata tttcatcaac ccaccaactc tatcttgaat gcaaacgatt aattgatagc
     7861 cactatcctc aatcacttac aattcaagat ttagcaaaag aactatccgt tcacagaagc
     7921 tacttatcaa gcgtattcaa agaatttaat accttatcac ccaaagaata cctactctac
     7981 gttcgaatgc accgagctag acaacttctc gaaaataccc aagagtccat caaggtaatt
     8041 gcatactcgg taggtttttc agatccactc catttttcga aagcttataa acaatacttt
     8101 aatcagactc caagtcatac aagaaaagaa tactctcaat accaactagt gagaaaggca
     8161 acattatgaa atcctaccaa gctgtctacc aaatcctatc taaagaaacc gactatatca
     8221 gcggagaaaa aatcgcagaa aaactatccc taagccgaac atcaatttgg aaagctatca
     8281 agcgactaga acaagaaggc attgaaattg atagtatcaa aaatagagga tataaactga
     8341 tgaatggtga ccttattctt ccagagattc tagaagaaaa tcttccaatt aaagtcagct
     8401 ttaaacccga aacaaaatca acacaactag atgcaaaaga agcaattgat ttaggccatg
     8461 aagcaaatac cctctatcta gcttcctatc aaacagcagg ccgaggccgt tttcaacgtt
     8521 ccttctactc accacaaggt ggtatttata tgacactcca tcttaaacca aatctcccct
     8581 atgacaaatt accatcctac acactacttg tagctggagc tgtctacaaa gccattaaga
     8641 acctaacttt aatagatgtc gacataaaat gggtcaatga tatctatcta aacaatcata
     8701 aaattggagg aatccttact gaagcaatga cctctgtaga aactggctta gtcacagata
     8761 tcattattgg agtaggtatc aatttcacta ttaaagactt ccctcaggaa ttaaaagaaa
     8821 aagctgccag cttatttaaa gctacagctc ctataacaag gaatgaattg atcatagaaa
     8881 tctggcgtac tttcttcgaa acaccagcag aagagctatt atacctatac aaaaaacagt
     8941 cattcattct aggaaaagaa gtcactttca cactagagca aaaagactac aagggacttg
     9001 ctaaagacat ctcagaaaat ggaaaacttt tagttcaatg tgataacgga aaagaaatct
     9061 ggctgaatag tggcgaaatt tctctcaata gttggaagta aaataacaca attataatat
     9121 aaacgatata aaaataactt cagattagta attcaattaa gttttacgga tctgaagttt
     9181 tattgtctct aaaaataaaa aagagagtta caaactctca ttaaaacgga gaataaggga
     9241 ttcgaaccct tgcgccagtt acccgaccta acgatttagc aaaccgtcct cttcagcctc
     9301 ttgagtaatt ctccaattaa tgggcacgag tggactcgaa ccaccgacct cacgcttatc
     9361 aggcgtgcgc tctaaccacc tgagctacgc gcccaagtta aaaaacttgg taatttgaac
     9421 aaagttcaaa gcgggtgacg agaatcgaac tcgcgacaac agcttggaag gctgtagttt
     9481 taccactaaa ctacacccgc ataaatacta tcaataaaat ggcgcgagac ggaatcgaac
     9541 cgccgacaca tggagcttca atccattgct ctaccaactg agctaccgag ccttattgcg
     9601 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cgagctgctc
     9661 catcccgcgt taataatata aaaggaggat gtgggattcg aacccacgca cgcttttaca
     9721 cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaatcct ccaatattca
     9781 aatggacctt gtaggacttg aacctacgac cactcggtta tgagccgaga gctctaacca
     9841 gctgagctaa aggtccgaca agatcattat agcggcgaag gggatcgaac ccccgacctc
     9901 ccgggtatga accggacgct ctagccagct gagctacacc gccatgaatc gggaagacag
     9961 gattcgaacc tgcgacacct tggtcccaaa ccaagtactc taccaagctg agctacttcc
    10021 cgagttaaat agaaaaatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
    10081 caggtactct atccagttga gctaagggtg ctccatatta tgccgaggac cggaatcgaa
    10141 ccggtacgat cgttaccaat cgcaggattt taagtcctgt gcgtctgcca gttccgccac
    10201 cccggcctct ctaagcgaac gacgggattc gaacccgcga cccccacctt ggcaaggtgg
    10261 tgttctacca ctgaactacg ttcgcactgt tttcttctat ctaaaaatgc cggctacatg
    10321 acttgaacac gcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc
    10381 tcatttgtta tatcttaatg cgggttaagg gacttgaacc cccacgccgt taagcgccag
    10441 atcctaaatc tggtgcgtct gccaattccg ccaaacccgc atatatgacc cgtactgggc
    10501 tcgaaccagt gacccattga ttaaaagtca attgctctac caactgagct aacgagtcta
    10561 aaataacttg cgttacctta aacggtcccg acgggaatcg aacccgcgat cttcgccgtg
    10621 acagggcgac gtgataaccg ctacactacg ggacct
//
LOCUS       DAVMEF010000060        10414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMEF010000060 DAVMEF010000000
VERSION     DAVMEF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.060"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            50..1978
                     /gene="glgB"
                     /locus_tag="VYQ30_001128"
     CDS             50..1978
                     /gene="glgB"
                     /locus_tag="VYQ30_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1966242.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            1968..3110
                     /locus_tag="VYQ30_001129"
     CDS             1968..3110
                     /locus_tag="VYQ30_001129"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1966243.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3100..4239
                     /gene="glgD"
                     /locus_tag="VYQ30_001130"
     CDS             3100..4239
                     /gene="glgD"
                     /locus_tag="VYQ30_001130"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1966244.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKSAKVTEDIHS"
     gene            4236..5669
                     /gene="glgA"
                     /locus_tag="VYQ30_001131"
     CDS             4236..5669
                     /gene="glgA"
                     /locus_tag="VYQ30_001131"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1966245.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5728..6376
                     /gene="serB"
                     /locus_tag="VYQ30_001132"
                     /pseudo
     CDS             5728..6376
                     /gene="serB"
                     /locus_tag="VYQ30_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            6385..7500
                     /locus_tag="VYQ30_001133"
     CDS             6385..7500
                     /locus_tag="VYQ30_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1966246.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTYGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLDLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMYKI"
     gene            complement(7497..7943)
                     /locus_tag="VYQ30_001134"
     CDS             complement(7497..7943)
                     /locus_tag="VYQ30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1966247.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8107..9411
                     /gene="eno"
                     /locus_tag="VYQ30_001135"
     CDS             8107..9411
                     /gene="eno"
                     /locus_tag="VYQ30_001135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1966248.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9586..10414)
                     /locus_tag="VYQ30_001136"
                     /pseudo
     CDS             complement(9586..10414)
                     /locus_tag="VYQ30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3114 a         1855 c         2319 g         3126 t
ORIGIN      
        1 aggtagtata ataaaaacat agaaaacgct tacaggagaa ggggagtata tggataatag
       61 agaagcatta aaaaccttta tgacgggtga aaatttttat ctccaacatt atctaggagc
      121 acatagggaa gaactaaatg gagagcatgg ctataccttc cgtgtttggg cacctaatgc
      181 tcaggctgtt cacttggttg gtgattttac caactggatt gaaaatcaga ttccaatggt
      241 aagaaatgat tttggggtct gggaagtctt taccaatatg gctcaagaag ggcatattta
      301 caaatatcat gtcacacgtc aaaatggtca tcaactgatg aagattgacc cttttgctgt
      361 caggtatgag gctcgtccag gaacaggggc aatcttaaca gagcttcctg agaagaaatg
      421 gaaggatgga ctttggctgg cacgaagaaa acgttggggc tttgaagagc gtcctgtcaa
      481 tatttatgaa gttcacgctg gatcatggaa aagaaattct gatggcagtc cttatagttt
      541 tgcccagctc aaggatgaac tcattcctta tctcgttgaa atgaactata ctcatattga
      601 gtttatgccc ttgatgtccc atcctttggg cttgagttgg gggtatcagc ttatgggtta
      661 cttcgcttta gagcatgctt atggccgacc agaggagttt caagattttg tcgaggagtg
      721 tcatacccat aatattgggg ttattgtgga ctgggtacca ggtcacttta ccatcaacga
      781 tgatgcctta gcctattatg atgggacacc gacttttgaa taccaagacc ataataaggc
      841 tcataaccat ggttggggtg cccttaattt tgaccttgga aaaaatgaag tccagtcctt
      901 cttaatttct tgcattaagc attggattga tgtctatcat ttggatggta ttcgtgtgga
      961 tgctgttagc aacatgctct atttggacta tgatgatgct ccatggacac ctaataaaga
     1021 tggcggaaat ctcaactatg aaggttatta tttccttcag cgcttgaatg aggttattaa
     1081 gttagaatat ccagatgtga tgatgattgc agaagaaagt tcgtctgcga ccaagattac
     1141 gggaatgaaa gagattggtg gtctaggatt tgactacaaa tggaacatgg gctggatgaa
     1201 tgatatcctc cgtttctacg aagaagatcc gatctatcgt aaatatgact ttaacctggt
     1261 gactttcagc tttatgtatg ttttcaagga gaattatctc ttgccattct cgcacgatga
     1321 agtggttcat ggcaagaaga gtatgatgca taagatgtgg ggagatcgtt acaatcaatt
     1381 tgcaggcttg cgcaatctct atacgtacca aatttgtcac cctggtaaga aattgctctt
     1441 catgggtagc gaatacggtc aattcctaga atggaaatct gaagaacagt tggaatggtc
     1501 taacctagaa gacccaatga atgctaagat gaagtatttc gcttctcagc taaaccagtt
     1561 ttacaaagat catcgctgtc tgtgggaaat tgataccagc tatgatggta ttgaaatcat
     1621 tgatgcggat aatcgagacc agagtgttct ttcctttatt cgtaagggta aaaagggaga
     1681 aatgttagtc tgtatcttta atatggtacc tgttgagcgg aaagatttta caatcggact
     1741 acccgttgca ggaatttacg aagaagtatg gaatactgag ttggaagagt ggggaggcgt
     1801 ttggaaagaa cataatcaaa ctgttcaaac gcaagaagga ctatggaaag attatgagca
     1861 gaccttaacc tttaccctac cggctatggg agcaagtgta tggaaaatca aacgtcgctt
     1921 gaaatctact aaaaccgtca caaataaaaa ccaaaaagga gtagaaaatg aaaaatgaaa
     1981 tgttagcttt gattcttgct ggtgggcaag gaactcgtct cggtaaactc actcaaagca
     2041 tcgcaaaacc agctgtgcaa tttggtgggc gctaccgtat cattgacttt gccctatcaa
     2101 actgtgccaa ctcagggatt cacaatgttg gggtcgttac acagtatcaa ccacttgctc
     2161 tcaacaacca tattgggaat ggttcaagct ggggactaga cggtattaat tcaggtgtct
     2221 ctattcttca accttattct gcaagtgaag gaaatcgttg gtttgagggg actagtcacg
     2281 ctatttacca aaatatcgac tatatcgaca gtgtcaatcc tgagtatgtc ttgattttgt
     2341 ctggggacca catctacaaa atggactatg atgatatgct ccagtctcat aaggataata
     2401 atgccagctt gacagtagca gttttagacg tccctcttaa agaagcaagc cgttttggta
     2461 tcatgaacac agatgctaac aatcgtattg ttgaatttga agaaaaacca gctcaaccta
     2521 aatctacaaa agcttctatg ggaatctaca tttttgattg gcaacgcctt cgtaatatgt
     2581 tagtcgctgc tgaaaagagc aaggttggca tgtcagactt tggtaaaaat gtcattccaa
     2641 attaccttga gtcaggtgaa agtgtttatg cttacgaatt tagtggttat tggaaagatg
     2701 ttggtactat tgagtcactt tgggaagcga acatggagta tatttctcca gaaaatgcct
     2761 tggatagtcg taaccgtcaa tggaagattt actcaagaaa cttgatttca ccaccaaact
     2821 tcctcggggc aaatgctcat gtggaagact cattagttgt agacggatgt ttcgttgatg
     2881 gaactgttaa acattctatc ctttcaacag gcgcgcaagt tcgcgaagga gcggaagtcc
     2941 ttgattcagt tatcatgagt ggagctatca ttggtcaagg agctaagatt aaacgtgcca
     3001 ttattggtga aggtgcgatt atttctgacg gtgtcgaaat tgatggaaca gatgaagtac
     3061 aagttgtagg atataatgaa gtagtggggg tagcaacaga tgaagattga taaatattct
     3121 gccattttag gaaatacagt tggttttcac aatatgtcga cattgacgga ccaccgtcca
     3181 gtagcaagtt tgccatttgg tgggaaatat cgtttgattg acttcccact ttcaagcctt
     3241 gctaatgcag gtgttcgtag tgtctttggt attttccagc aggataatat cagctcagta
     3301 tttgaccata ttcgttcagg acgcgagtgg ggcttatcaa cccttcttag ccattactat
     3361 ctaggaattt acaatacccg tgtagaaagt agtacagttg gaaaagaata ctaccaacag
     3421 cttcttactt atttgaaacg ttctggctca aaccaaacgg ttgccctcaa ctgcgatgtt
     3481 cttattaaca ttgatttgaa ccaagttttc cacctacata gtacaacaaa agagcctatc
     3541 actgtagttt ataaaaaact agctaagaaa gatatttcag aagtaaatgc aatcttggat
     3601 gtagatgaaa cagaccatgt tctttctcat aaactctttg atagcaagtc aacagctgaa
     3661 acgttcaata tgtctacaga tatctttgtc gttgatacac cttggttgat tgaacacttg
     3721 gaagaagaag ctaaaaaaga acatccagag aaattgcgct atgttttacg ggatttggct
     3781 gtaaaagagg gagctttcgc ctacgagtac acgggctatc tagccaatat tcactctgtt
     3841 aaatcttatt atcaagcgaa tatcgatatg cttgaatcac aaaaattcta ttctcttttc
     3901 tcaccaaacc aaaagattta tacaaaggtc aaaaacgaag agccaactta ctatgctaat
     3961 acatctaagg taagcacttc tcagtttgcc tctggtagta ttattgaagg tcaagtagct
     4021 aattctgttc tatcacgtaa tattcatgtt cataaggata gcttggttaa agatagcctg
     4081 ctcttccctc gtgttgttat tggagaaggg gctcaggtcg aatatgctat cttggacaaa
     4141 ggggttgaag ttgagcctgg tgttgtgatt cgaggaactg cagaacatcc agttgtggtt
     4201 aagaaaagtg ctaaagtaac agaggatatt cattcatgaa aattttattt gtagcagcag
     4261 agggtgcacc cttttcaaaa acaggtggtt tgggagacgt cattggcgct cttccaaaat
     4321 cactggtaaa agctgggcac gaagttgcag tgattttacc ctactatgat atggtagagg
     4381 ctaaatttgg aaatcagatt gaagatgtgc ttcattttga ggtgagcgtt ggttggcgca
     4441 gacagtattg tggaattaag aaaacagtat taaatggtgt aaccttctac tttattgaca
     4501 atcaatatta tttcttccgt ggtcatgttt acggtgattt tgatgacgga gaacgctttg
     4561 cctttttcca actggctgcc attgaggcta tggaaaggat tgactttatt cctgatcttc
     4621 tccatgttca tgactaccat acagctatga ttcctttctt gttgaaggaa aaataccgtt
     4681 ggattcaagc ctatgaggac attgaaacag ttttaaccat tcataattta gaattccaag
     4741 gacaattttc agaaggaatg ttgggtgatt tgtttggagt tggctttgaa cgttacgctg
     4801 atggcaccct tcgatggaac aactgtctga actggatgaa ggcaggtatt ctctatgcga
     4861 accgtgtttc aaccgtttca cctagctatg ctcatgaaat tatgactagt cagtttggat
     4921 gtaatttgga tcagattctt aaaatggagt ctggtaaagt atctggtatc gtgaatggga
     4981 ttgatgctga tctttataat cctcagacgg atgctctttt agactatcat ttcaatcagg
     5041 aagatttgtc tgggaaagcc aaaaataagg caaaattgca agaaagagtt ggcttgcctg
     5101 ttagagctga cgttccactg gtgggaattg tttctcgttt gacacgtcaa aaaggttttg
     5161 atgtggtggt cgaaagtctt caccatatct tgcaagaaga tgttcagatt gttcttttgg
     5221 gaactggcga tccagccttt gaaggagctt tctcatggtt tgctcagatt tacccagaca
     5281 agctatcagc aaatatcact tttgatgtca aacttgctca ggaaatctac gctgcttgtg
     5341 acctcttcct catgccaagt cgttttgaac cgtgtggctt gtctcaaatg atggctatgc
     5401 gttatggaac cttgccattg gtccatgaag ttggaggctt gcgagataca gttcgcgctt
     5461 tcaatccaat cgaaggaagc ggtactggct ttagctttga caatctatct ccttattggt
     5521 taaattggac tttccaaaca gcattggact tgtatagaaa ccatccagac atttggagaa
     5581 acctacaaaa acaagctatg gagagtgact tctcatggga tacagcctgc aagtcatacc
     5641 ttgacttgta ccatagttta gttaattaat agataaaatc gtatgatgac ttcatacgat
     5701 ttttaggctg tttagagagg agaactgatg tctcaagtaa aaggcttgtg tgttttggat
     5761 gtagatggaa ccttaatcct agaagaagtg attgatcttt tagggagaga ggcaggtcat
     5821 gaggcggaaa tttcgcagat tacaagtcgg gcaatgcgag gagagttggt ctttgaaagc
     5881 agtttaagaa aaagagtgtc cttgttggaa ggtcttccta ttttggtctt tgataacgtc
     5941 ttcaactcaa ttcatctatc gctaaatgtc ccagagttca tctctattct ccaaaagaat
     6001 ggcatcctag ttggcctggt gtgtggtgtc tggtggatac caatagttgg agagattagc
     6061 aaaatccctt ggtattgcct atttcactgc caaccagctt gaagtcaaag aaggtctttt
     6121 aacaggaaaa ttagttgaac aaattataag tccccaggtc aaaaaagaga ctctggaaaa
     6181 atggagaaag aaactaaaac tttctaaaga aagaacggtg gcaatcggtg atggggtcaa
     6241 taatctatta atgttgaagt cggcggagtt aggaatcgcc ttttgtgcca aagaagtgct
     6301 caaaaaagaa ataccacatc atgttgacaa gagggatttt ttagaagttc ttcctttgat
     6361 tgactgttta gaatgaggga aaatatgaag attgtaattg caccagattc gtttaaggaa
     6421 agcttgacag ctcaacaggt agctgaagca ataaaaagag gcttccaaca atcgatagca
     6481 gatgtagaat atctcctctg ccctgttggt gatgggggag aaggtactgt agatgctatc
     6541 cgacattctc ttgacctaaa agaaaaatgg atccaagtga cagacccttt tggacaaaaa
     6601 gaagccatgc gctattttca aaaaggggaa ctggcactat ttgaagtagc tgacttggtt
     6661 ggtcttggaa aaattccgct agagaaacga aatccacttc aaatccaaac ttatggtatt
     6721 ggagagttga ttctccatct catttctaaa gggattaaag atatttatat cggtgttggt
     6781 ggcacggcca gtaatgatgg aggactgggg attgctgctg gtttaggtta tcaattttat
     6841 gatagggatg gaaatgtctt gcctgcttcc ggtcaatcct tattagactt agcttctgtt
     6901 tcaacagaaa attgctataa aattcctgaa ggtgttcaaa ttcatattct agcagatgtc
     6961 gtgagtccct tatgtggtca tcaaggtgcg acttacactt ttggcaatca aaaaggtcta
     7021 catccgacta tgtttgcagt cgtagatcag gcgatccaag atttttatga aaaattctca
     7081 cctgcaacat tagaaattaa aggagcagga gcaggtggag gccttgctgg tggtttgtgt
     7141 gcctttgctc aggcaagtat cgtgtctgga attgatacct gcttggactt aatcaacttt
     7201 gataagaaag tttcagatgc tgacttggtt gttgttggag aaggaagact tgatagtcaa
     7261 agctttgctg ggaaagcgcc tataggtgtt gcaaaaagaa cccctgttgg agttcctgtt
     7321 attgctattt gcggtagtct tgctgaagat ttacctcccc taccatttga aaatatccaa
     7381 gctgtctttt ctattttgga gaaaagtgaa cctttagaag atagtttaaa aaatgccagt
     7441 ctctatctgg agcgcacggc tgccaatatt gggcacttat taaatatgta caagatttag
     7501 ctaaaccatt tcttccataa ggatgttttg gctggttctt cctttttaac ttcccaaagt
     7561 tttggaaaag caagtcgaag gtctttttct tctacttgaa ctggtgcatt gctatgaata
     7621 accaggccaa aaggagaagt aatatgctct tcagatacta tggtggcttg actatcagtt
     7681 tcttttgctt cttttaagta gaaaacttgc ttatcaaatt cgatagttgg tgaaatcttc
     7741 acaaatagtg gctctgcttt ttcctgaagg ttttctaaaa taaataaaaa gcctttttct
     7801 aactgagggc tatttgctgt gtcaatatct acatatccaa gaactctttc ctcaaaagta
     7861 ccaagatagc gtctttgctc atccggattt atttttaacc caccatgagc tttttcaagt
     7921 aattgttttg ataaatctgt catgaaaaca gtatatcata aaagttagaa taaaacagac
     7981 aaaagaaagc gattacttta taaagttaag gaaaagttgc acaaagataa cgttttttct
     8041 tgaaaaagga ggggttttaa ggtattatag aggtgtaaaa aatttaactc ataaggagag
     8101 taaaaaatgt caattattac tgatgtttac gctcgcgaag tcctagactc acgcggtaac
     8161 ccaacacttg aagtagaagt ttacactgaa tcaggtgctt tcggacgtgg tatggttcca
     8221 tcaggagctt ctactggtga acacgaagca gttgaacttc gcgacggtga caaatctcgt
     8281 tacggtggtc ttggtacaca aaaagctgtt gacaacgtaa acaacatcat tgctgaagct
     8341 atcattggct acgatgtacg tgatcaacaa gctattgacc gtgctatgat cgcacttgac
     8401 ggtactccta acaaaggtaa attgggtgcg aatgcaatcc tcggtgtgtc tatcgctgta
     8461 gctcgtgctg ctgctgacta ccttgaaatc ccactttaca gctaccttgg tggattcaac
     8521 actaaagttc ttccaactcc aatgatgaac atcatcaacg gtggttctca ctctgacgct
     8581 ccaatcgctt tccaagagtt catgatcttg ccagttggtg cgccaacatt taaagaagcc
     8641 cttcgttacg gtgctgaaat cttccacgct cttaagaaaa tccttaaatc acgtggtttg
     8701 gaaactgccg taggtgacga aggtggattc gctcctcgtt tcgaaggaac tgaagatggt
     8761 gttgaaacta tccttgctgc gattgaagct gctggatatg taccaggtaa agacgtattt
     8821 ctcggatttg actgtgcttc atcagaattc tacgataaag aacgtaaagt ttacgactac
     8881 actaaatttg aaggcgaagg tgctgctgtt cgtacatctg cagaacaaat cgactacctt
     8941 gaagaattgg ttaacaaata cccaatcatc actattgaag atggtatgga tgaaaacgac
     9001 tgggatggtt ggaaagctct tactgaacgt cttggtaaga aagtacaact tgttggtgac
     9061 gacttcttcg taacaaacac tgactacctt gcacgtggta tccaagaagg tgctgctaac
     9121 tcaatcctta tcaaagttaa ccaaatcggt actcttactg aaacttttga agctatcgaa
     9181 atggctaaag aagctggtta cactgctgtt gtatcacacc gttcaggtga aactgaagat
     9241 tcaacaatcg ctgatatcgc agttgcaact aacgcaggac aaatcaagac tggttcactt
     9301 tcacgtacag accgtatcgc taaatacaac caattgcttc gtatcgaaga ccaacttggt
     9361 gaagtagctg aatatcgtgg attgaaatca ttctacaacc ttaaaaaata aaatagttca
     9421 gtgaactatt ttatcccgaa ccatgaaatt caaaagttcg gccgttgatt taacaacgtt
     9481 tctagcccct cggattttat ccgaagggct atttttgtat ttagagggca aaaaagggac
     9541 aaaacttttt aggctctttg tcaactgtag tgggttgaaa aaaagctaag ctcgagaaag
     9601 gacaaatttt gtcctttctt ttttgatatt cagagcgata aaaatccgtt ttttgaagtt
     9661 ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt ttgatgagat tattggtcgc
     9721 ttccaatttg gcgttagaat agggtagttg aagggcgttg acgattttct ctttgtcctt
     9781 tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc tgctttagat tgtcctcaat
     9841 gagtccgaaa aatttctccg gttccttatt ctgaaagtga aacagcaaga gttgatagag
     9901 ctgatagtga tgtttcaagt cttgtgaata gctcaaaagc ttgtttaaaa tctctttatt
     9961 ggttaagtgc atacgaaaag taggacgata aaatcgctta tcactcagtt tacggctatc
    10021 ctgttgaatg agtttccagt agcgcttgat agccttgtat tcatgggatt ttcgatgaaa
    10081 ctgattcatg atttggacac gcacacgact catagcacgg ctaagatgtt gtacaatgtg
    10141 aaagcgatca agaacgattt cagcattcgg gagtgaaaca gtctgggaga ctgtttcagc
    10201 ctgagcctag aaatttgaaa gcgaagctgt ttagccaagt catagtaagg gctaaacata
    10261 tccatagtaa taattttgac gcgacatcgg acaactctat cgtagcgaag aaagtgattt
    10321 cgaatgatag cttgtgttct accctcaaga acagtgatga tattgagatt gttaaaatct
    10381 tgcgcaatga agctcatctt tccctttgta aaag
//
LOCUS       DAVMEF010000061        10143 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMEF010000061 DAVMEF010000000
VERSION     DAVMEF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.061"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1145..7990
                     /locus_tag="VYQ30_001137"
                     /pseudo
     CDS             1145..7990
                     /locus_tag="VYQ30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            8085..8288
                     /locus_tag="VYQ30_001138"
     CDS             8085..8288
                     /locus_tag="VYQ30_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966249.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            8291..8641
                     /locus_tag="VYQ30_001139"
     CDS             8291..8641
                     /locus_tag="VYQ30_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966250.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            8651..9067
                     /locus_tag="VYQ30_001140"
     CDS             8651..9067
                     /locus_tag="VYQ30_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1966251.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            9071..9403
                     /locus_tag="VYQ30_001141"
     CDS             9071..9403
                     /locus_tag="VYQ30_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1966252.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            9407..>10143
                     /locus_tag="VYQ30_001142"
     CDS             9407..>10143
                     /locus_tag="VYQ30_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1966253.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWCYFDGSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIA"
BASE COUNT         3284 a         1923 c         2414 g         2522 t
ORIGIN      
        1 acttagtcgg actaagaatg aactgtctgg cgtgaagtca gcgcaagcga cgtatgagga
       61 gacgacgact cgtagactgt cagaactgac caacttggcc aatggtaaag ccagcaagtc
      121 agaactcacg caaacagctg aggagctggc tagtcggatt gcgagtgtgc aggcaggtag
      181 ttcacggaat tacttcagga attcacgttc aagaacgttc acaacaggag gtcaagcggt
      241 atacgactat cgaacattca tagttcctga tttctggaag aacagtgaca ggttcaagcg
      301 tgattatgtt cgcatatctt ttgatgtgac tttccctgtc gccctagtaa atgacatgcc
      361 tgctatggtg cattttagtg ctcatccatg gtatgcctac agaaacttaa tttttaaagg
      421 tggaactgtc gaacgccaac attttgagtt tacgattgac ttgtctagtt cttctgagga
      481 ctatcagact aataatgtgt tcattcgttt tggtactaat tatggatttc ctgctggtct
      541 gcaggtcgtc attgagaacg ctatgttatc ggttggtaat tattttccag cctatcaacc
      601 agcgtatgaa gaccaagaag accgtgtctc agtagtcgaa tccaacttta agcagcgtgc
      661 tgattcactc gacgctggtg taagccgtct gactgaaggc cttagaacta aagccgatat
      721 cagctcactc aatgtgactg ctgaaaatat ccggcaatct gtgaagagcc ttgagacaga
      781 tacgcagaac aagctaaatc agaagttgag tcaggctgaa tttgaggtgc gagccggctc
      841 tatccgtcag gaaatcctga acgcaaccaa ggataaagcc agcaagtcag aactcacgca
      901 gacagctgag gagctctcta gtaagatagc gagtgtgcag gcatccggtc gaaatctatt
      961 cttgaactca ctattcaagc aggatatttc aaaaacagga atttggacaa cgagtacata
     1021 tacggctgct atcgatagcg aaagtaagta tcttggacac aaggctctta aaattatagg
     1081 tctgaatcca tctggccgtg atggaggtaa tcccaaggtt acttatccag ctctgggtca
     1141 attcgggaaa gtaattcccg gaagtacgac taatcaagat gtaaccatta gtttttatgc
     1201 taaggcaaat aaaaatggaa taatgctaag atctcgatta gggaatatcg gatataaaac
     1261 tggaaatgtg acattgtcga cagaaattaa gcgatatgtt gtccatattc caaaaggttg
     1321 gacaaacgaa tccaagcaga ccacaaatga atggttgttc aatttcaacc aggaaggaac
     1381 cgtttggatt tggatgccga agtttgaaat aagcgatgta gatacttctt attcagaagc
     1441 tcctgaagat atagaaggtc agatttcaac agttgaatcg acctttaaac aacgagccaa
     1501 ctcgctcgaa gctggtgtaa gccgtctgac tgaaggtctt agaaccaaag ccgatatcag
     1561 ctcactcaat gtgactgctg aaaatattag gcagtcggtg aagagtcttg agacagacac
     1621 gcagaacaag ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ctggctctat
     1681 ccgtcaggaa atcctgaatg caaccaaaga taaagccagc aagtcagaac tcacgcagac
     1741 agctgaggag ctagctagta agatagcgag tgtgcactta gggcgcagaa atctgctgaa
     1801 aggcacaaaa gagcttgcga gatacaagcc ggttagtgaa tataatggtt ttaaagttat
     1861 cagaacagtc gcaggagcaa ctagatatca ggatagctat gtggaaagaa ccgttatacc
     1921 aacggctggg acagagtata tagctatctt ttatgcacga gccagtgaaa atgactatcc
     1981 tgtgcgctgt catttttaca atcctaacac ggttgtatca tcagaaaaca gcagcggata
     2041 taagtcaagg tcgtcagatg gcttgtctat tatccgtctc tcgacagact ggcagttgtg
     2101 ctgggttaaa tggacccaaa ccgcaacaga tcaagccaag acggtcatca ttggccgcca
     2161 tggccctcaa gtaggcggta aagagggggt atgggttgaa atctgcgccc ctgccatttt
     2221 tgagggaaat cttgcaggtg actggtcacc agcatacgaa gaccaagacg aacgtgtctc
     2281 agcggtcgaa tccaacttta aacagcgtgc tgattcactc gaagctggtg taagccgtct
     2341 gactgaaggc cttagaacca aagccgatat cagctcactc aatgtgactg ctgaaaatat
     2401 ccggcaatct gtgaagagtc ttgagacaga cacgcagaac aagctaaatc agaagttgag
     2461 tcaggctgaa tttgaggtgc gagccggctc tatccgtcag gaaatcctga atgcaaccaa
     2521 ggataaagcc agcaagtcag aactcacgca gacagctgag gagctctcta gtaagatagc
     2581 gagtgtgcag gtcgggggta ggaattatat ccggggtaca aagcgcatga tgctagccag
     2641 aggattgtgg gcatcaggta cttttagacc gtcaggcgct gggacggcaa agacgattga
     2701 tgtatcagac agtccagtaa ctggctttga taaagcgata cgattgacct caagcaatgc
     2761 tagagaccaa ataggtattg ctcaagatgg attttatatc tcgcaaggca catacacgat
     2821 gtcttgttgg gtcaaaggca gaagaggtca aaaggtcaag ctacaaactt attggcaagt
     2881 caatgataat tcgggtattt cgcccatctt tacattaaag gatgaaaatt ggacaaagct
     2941 atcgtttact agcgctagaa atagggctgg agtcgcatca attggctatg tgtatctcgt
     3001 aaatgctgaa gtcggagaat atttagatgt tcttgcgccc cagctggaag acggaagttt
     3061 ggcaacaagc tcaaaagaag ctcctgaaga tatagaaggt cagatttcaa cagtagaatc
     3121 gacctttaaa caacgagcca actcgctcga cgctggtgtg agaagcttga ctgaagggct
     3181 tagaaccaaa gtggatatca gctcactcaa tgtgactgct gaaaatatcc ggcaatctgt
     3241 gaagaggctt gagacagaca cgcagaacaa gctaaatcag aagttgagtc aggctgaatt
     3301 tgaggtgcga gctggctcta tccgtcagga aatcctgaat gcaaccaagg ataaagccag
     3361 caagtcagaa ctcacgcaga cagctgagga gctctctagt aagatagcga gtgtgcaggc
     3421 atccggtcga aatctattct tgaactcact attcaagcag gatatttcaa aaacaggaat
     3481 ttggacaacg agtacatata cggctgctat cgatagcgaa agtaagtatc ttggatataa
     3541 cgctcttaaa attataggtc tgaatccatc tggccgtgat ggagggaatc ccaaggttac
     3601 ttatccagct ctgggtcaat tcgggaaagt aattcccgga agtacgacta atcaagatgt
     3661 aaccattagt ttttatgcta aggcaaataa aaatggaata atgctaagat ctcgattagg
     3721 gaatatcgga tataaaactg gaaatgtgac attgtcgaca gaaattaaac gatatgttgt
     3781 ccatattcca aaaggttgga caaacgaatc caagcagacc acaaatgaat ggttgttcaa
     3841 tttcaaccag gaaggaacca tttggatttg gatgccgaag tttgaaataa gcgatgtaga
     3901 tacttcttat tcagaagctc ctgaagatat agaaggtcag atttcaacag tagaatcgac
     3961 cttcaaacaa cgagccaact cactcgaagc tggtgtaagc cgtctgactg aaggccttag
     4021 aaccaaagcc gatatcagct cactcaatgt gactgctgaa aatattaggc agtcggtgaa
     4081 gagtcttgag acagacacgc agaacaagct aaatcagaag ttgagtcagg ctgaatttga
     4141 ggtgcgagct ggctctatcc gtcaagaaat cctgaacgca accaaggata aagccaacaa
     4201 gtcagaactc acgcagacag ctgaggagct ctctagtaag atagcgagtg tgcaagtcgg
     4261 aggaattaac ctcttgcgta atactgcgag tttgttgatt ggcgatcgtt caaaaggatg
     4321 ttggatgagt tcaagcggag gaaatgggcg agcgattagc gtagaagttt tggctcctcc
     4381 caaaaaaatg ataaaaaaca tgattcgtgt tattgaaaat acgaatggtg gaaataaaga
     4441 tttaactcaa cttgttagat tgcgaattgg tgaaaagtac acgatttctt gttatgcaag
     4501 ggttgctagc gatagcccaa atgcaaacgt gaacttgcta tttcgttcgt gggcaaatga
     4561 taccgattta aatcgcaaat ttcagaaatc catctctcat aaaaattggc aaaaatattc
     4621 atttacattt actgctgatg caattgaaaa ttcaattcaa tttggacaat ctggcgcagg
     4681 aattatcgaa atatgcgctc cgaaaatcga atcaggaacg ttagcgactg attacagcga
     4741 agctcctgaa gatatagaag gtcagatttc aacagttgaa tcgaccttca aacaacgagc
     4801 caactcactc gacgctggtg taagaagctt gactgaaggg cttagaacca aagtggatat
     4861 cagttcactc aatgtgactg ctgaaaatat ccggcaatct gtgaagagcc ttgagacaga
     4921 cacgcagaac aagctaaatc agaagttgag tcaggctgaa tttgaggtgc gagccggctc
     4981 tatccgtcag gaaatcctga acgcaaccaa ggataaagca gataagactt tagttgtatc
     5041 tgaagctggg aaattgcgtg aagaattttc aaaaatgaag gtgggaggcc ggaatctatg
     5101 gataaaatcc aagacggttg gagctgtaat tgaaaaatta cctgaaaacc acgtcacagg
     5161 tcaaaaagaa tgctataggc tagagaacaa ctctacttta acgttcaacc ttgaaccaga
     5221 tttcagctca aggttgtacc aaaaagttac ttttagcgct tggatcaagt acgaaaatgt
     5281 agtccaaggt cgaaattttt ggaatgtatt taattgcttc aaacattatc tttttagaaa
     5341 aaatagtgag accggagtac agagtggtcc agattatgct acgcttggta tgtataaagg
     5401 ttcggcagat tggaaatata ttacattcac ttatgactac tctgaaaaaa caaattttga
     5461 tcaattgaag atatcattgc gattcaatct tgaaggtgct acaagcggta cagcttgggt
     5521 aacaggaatc aaggttgaaa tcggtagtgt ggcgacggac tggagtcctg cgcctgagga
     5581 cgctgatggt ctcatcactg aagctaaggc tacctttgag cggacagctc agggcttgcg
     5641 aaccgactta tcagctattc aggaatatgt aaataaagac ggtcagcgac aggaagccct
     5701 acagcgctat actcgtgagg agagcacgag acaagcgaca gcagtccgtg agctggtcaa
     5761 tcgtgatttc gttggtaagg ctacttatca agaagatgtt aagggtatca atcagaggat
     5821 tgaagctgtt aaaactagtg cgaataaaga catcgctagt caaatcgcta gctatcgtca
     5881 atctgtagat ggtaagttca cggatatttc aagtcagata actacttata agcaagatgt
     5941 gggcggtcaa atcagtggtc tatcaaatag acttacaagc agtgagcaag gaaccactac
     6001 tcagatttca aatatttcaa atcggataaa cagtaataag caaggcacag ataatcagat
     6061 ttcaaattta aagactcagg tcgctacaaa caaggataat gctgaacgac aaatgggtag
     6121 aatatctgat caggtttctg caaacaaagc gaatgctgat cgtcaatttg cgaatgtgac
     6181 caatcaacta gtgcgaaaag tagagactac tgacttccag cgtgttaagg aaaccagtaa
     6241 actttacgag cggattttgg gcaatactga aaatggaatt gcggataagg ttgctcgcat
     6301 ggctctgacc aatcaactgt ttcaggttga ggttgggaaa tatagtgtaa gcggccctaa
     6361 cctcattaag aatagtgatt ttaaaaatgg tacgaatgaa tggggctcaa ctcaaaattt
     6421 aggaagattg gttaagcata gcttttatca caacgggcag aaagacctta tgcgtttaag
     6481 taatgcaact aaaaacgaaa actttttgta tagtcaccgt tttaatcttg aacgaaatac
     6541 tgactatgta ctgaatttta gaggatttaa caacagtgct ctagcaagct atgatgttta
     6601 tattttggga cgaagagcag gcgagagcga tggattcaca atcgttaaga aagttgttag
     6661 cagcaagaaa ctatctacct ctagatgcga agatgtctca gtaactttta attccggaga
     6721 aatggataat gcttacattc gttttgataa caatggttca tcatcaggaa cagctgattt
     6781 gtatattaca gaagttgact tgtacaaagg ttataaacct agaacatggc aaccacatac
     6841 agaagatgca gtcgcagatg cgaataagaa gcttgaagca acgcaaacaa aaatgactca
     6901 actagctggc tcatgggtag ttgaaaacat caactcggct ggagatatca tctctggaat
     6961 caatcttggc gccaatggac ataaccgctt tgttgggaaa ttgacccaca tcactggaga
     7021 gaccctgatt gacagagcag tcatcaagtc tgccatggtt gataagctca aaacggccaa
     7081 ttttgaagct ggttcggtca cgactacgat attagacgct gaagcggtaa ctgctgagaa
     7141 gttgaaagtt gacgatgcgc ttattagaaa attaactgca aaagatgctt ttattgaccg
     7201 actgacatct aaacgtatct tctctactaa ggttgagtcc gtcatttcta gctcaacctt
     7261 cctagaagcc tatcaaggcc gaatcggtgg attcacactt ggtcaatttg accagggtgg
     7321 cggtcgctgg atttcaggtg tcaatcagtt ctctgttggt atggggaatg gtgccgggta
     7381 tggagtccgg acagccttct gggcgaactg gggaaataat tggaactatg ccggacctaa
     7441 agcatggaac gtcaatactg atgggaaaat gtactgtagg aatgaagtcg gtttttatga
     7501 tcaagtggat ttttcgaatt catcgagagc aaacttctat gggaatacta ctttttctcg
     7561 ttctcctgtg ttttcaaatg gtatcgaact tggaagtaaa gatgtgcttg gtgatggttg
     7621 gaatcccaaa ggcggaagga atgcggttgt ttggtggaat caggtcggta gcggtagctt
     7681 gaagtattgg atggaacaaa aatcagatag acgcttaaaa gagaacatca cagatacagc
     7741 tgtgaaagcc ttggataaaa tcaacagatt aagaatggtt gcatttgatt tcatcgaaaa
     7801 taaaaaacat gaggagattg gtctaatagc tcaagaggct gaaaccatcg ttccaaaaat
     7861 tgtctcacga gatcctgaga atccagatgg ctatctgcat atcgactata ccgctttagt
     7921 tccttactta atcaaggcta ttcaagaatt aaatcaaaaa atagaaaaaa tggagaaaac
     7981 aatagcatga ataacaacat gttgaccaat atcgcacttt aaagcaattc aggagcttgc
     8041 tcttgaaaat agaaaacgaa cacacagatt ggagaactta gaaaatgaac acagaacagc
     8101 ttaaccaagc cttacaaatg acaattagtg aaatgtcaac aacttcaaca aattcgatga
     8161 ttacaagtaa tatcttgagt attcagttga atgagcaaag ggaagaaaat caaagacttc
     8221 aagcacgagt ggatgagctg gaagctctgc ttgatgaaca aactaaacca gcagacaaag
     8281 gagaatagac atggcagaaa caattcaaaa cacagataac ttgctagacc ttacaaaaat
     8341 cacagaacca tttgatcttg cgagcgcttt gcgctatatg aaagaaaatg gagagttcat
     8401 tcgttgcaag aatgtaagcg atgacttcta tatgtatcgt gatgttcaaa aacgtcctgt
     8461 gatcgtaaat ggccgtcgcc aactcaagga tgttgaaacc gtttgggcgt tcaaccagtg
     8521 gggtggtaca atcgcaacaa tcaacgtcgc cgttctgttg aatcatgaat tctatatcat
     8581 gaaatttgat gcagagggca atcctgactg gacggatcca acggtaaaac ctaaagaata
     8641 ggaggtgtgt atgcaaattg aatttttcaa ttttctaaga agtgtcgtac agactgaaga
     8701 tggtttggtt ttgtacgctc tagcactgat tgtctcaatg gaaatcattg attttgtgac
     8761 agggacgatt gcggcgatta tcaatcctga catcgagtac aaaagcaaaa tcggcattaa
     8821 cgggctcctt cgtaagattt caggggttct cttactgatg atcctcattc cggcgtccgt
     8881 tttgttgcct gaaaagacag gttttgcatt cttgtactca atctatctcg ggtacatcgc
     8941 atttactttt caatctctca ttgaaaatta ccgcaaatta aaaggaaatg ttactctttt
     9001 tcagccgatt gtaaaagtat ttcagcgatt acttgaaaaa gatgatgata cgaaaaaagg
     9061 agaataacaa atgcaacaaa ttactgaaat cattactaat ggagcaatca gcatcctagt
     9121 cattttagca ggggtggtag ttagggcagt caaggactac ctggttcaaa aaggtggaga
     9181 aaagaccatc aaaatcgttg aaatcttggc caagaacgca gttaatgccg tggagcaggt
     9241 agctgctgaa actggctaca agggagatga aaaactggca caggctcgcg ctaaagtccg
     9301 tgctgagctt acaaaataca atattagcat gactgacaaa gacttagaca ccttcgtaga
     9361 gtcagcagtg aagcagatga atgacgcatg gaaaggacga tagggaatgg atatcgatag
     9421 aaacagacta cgtacaggct tgccacaggt tggggtgcag ccttatcgac aagtacatgc
     9481 tcactcaaca ggtaaccgca actcaaccgt acagaatgaa gcggattatc actatagaaa
     9541 ggaccctgaa ctagggttct tttcacatgt tgtcggaaac ggccgtgtca tgcaagtagg
     9601 acctgtgaac aacggaagtt gggatgttgg gggcggttgg aatgctgaga gttacgcagc
     9661 ggttgaactg attgaaagcc attcaactaa ggaagagttt atggctgact atcgcctcta
     9721 tatcgaattg ctacgcaatc tagcggacga agcaggtttg ccgaaaacgc ttgatacagg
     9781 gagtttagct ggaattaaaa cgcacgagta ttgcacgaat aaccaaccaa acaaccactc
     9841 agaccatgtg gatccatacc cttacttggc aaaatggggc attagccgtg agcagtttaa
     9901 gcatgatatt gagaacggct tgacgattga aacaggctgg cagaagaatg acactggcta
     9961 ctggtacgta cactcagacg gctcttatcc aaaagataag tttgagaaaa tcaacggtac
    10021 ctggtgttat tttgatggct caggctatat gcttgcagac cgctggagga agcacacaga
    10081 cggcaactgg tactggttcg acaactcagg cgaaatggct acaggctgga agaaaatcgc
    10141 tga
//
LOCUS       DAVMEF010000062        10036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVMEF010000062 DAVMEF010000000
VERSION     DAVMEF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.062"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            77..553
                     /locus_tag="VYQ30_001143"
     CDS             77..553
                     /locus_tag="VYQ30_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1966254.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            591..893
                     /locus_tag="VYQ30_001144"
     CDS             591..893
                     /locus_tag="VYQ30_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1966255.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            901..1705
                     /locus_tag="VYQ30_001145"
     CDS             join(901..1269,1271..1705)
                     /locus_tag="VYQ30_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1966256.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     GHDMKLFKMSCRNIGQAGKILADSDYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            1754..2218
                     /locus_tag="VYQ30_001146"
     CDS             1754..2218
                     /locus_tag="VYQ30_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1966257.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEEICSVPRLFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGSFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2375..2800
                     /gene="lacA"
                     /locus_tag="VYQ30_001147"
     CDS             2375..2800
                     /gene="lacA"
                     /locus_tag="VYQ30_001147"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1966258.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2831..3346
                     /gene="lacB"
                     /locus_tag="VYQ30_001148"
     CDS             2831..3346
                     /gene="lacB"
                     /locus_tag="VYQ30_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1966259.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3357..4286
                     /locus_tag="VYQ30_001149"
     CDS             3357..4286
                     /locus_tag="VYQ30_001149"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1966260.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4288..5268
                     /gene="lacD"
                     /locus_tag="VYQ30_001150"
     CDS             4288..5268
                     /gene="lacD"
                     /locus_tag="VYQ30_001150"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1966261.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSEPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            5502..6338
                     /locus_tag="VYQ30_001151"
     CDS             5502..6338
                     /locus_tag="VYQ30_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1966262.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6374..6691
                     /locus_tag="VYQ30_001152"
     CDS             6374..6691
                     /locus_tag="VYQ30_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1966263.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6691..8382
                     /locus_tag="VYQ30_001153"
     CDS             6691..8382
                     /locus_tag="VYQ30_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1966264.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            8591..9997
                     /gene="lacG"
                     /locus_tag="VYQ30_001154"
     CDS             8591..9997
                     /gene="lacG"
                     /locus_tag="VYQ30_001154"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1966265.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         3184 a         1800 c         2080 g         2972 t
ORIGIN      
        1 acagaaagta attgacaacg gtttcatata gtgttatact tatggcataa agttaatgga
       61 aagaagggaa aaacttatgg tattagatta tttctttgac aaaaaccttg tgttttgctt
      121 agaagcggat aatcaagaac aactctttga tcaggttgca actttattgg aagaacgaga
      181 aattgtcact ccaacttatc gtgaagcttt gatcacgcgt gaaaagtcat ttccaactgg
      241 tttagatatg gaatttctag gaaaggactt gccaaatgta gcgattcctc atacagatat
      301 tgttcataat ctagctgaga aagtggtggt tgttcgatta gaaaaaccag taacttttca
      361 caatatgata gctcctgata aggaagtaga agtatccttg ctcttcttta tcatcaataa
      421 ctcaagttca agtcaaacaa atattctggc tcagttgatg gactttttca caggaaatgg
      481 acatcttgaa gacctatcaa aaatttccga accagaaaaa ctttatgctt acattgctga
      541 aacaaccgct taatcttgtc tattaaaaaa taaaatcgga ggaaatctaa atgattaaaa
      601 ttcttgctgc ctgcggtgca ggtgttaact caagtcacca aattaaaagt gctctagaag
      661 aagaactttc aaaccgtggt tatgatgttc actgtgatgc agtcatggtg aaagatgtaa
      721 acgaagacct tatgaaaggt tacgatatct ttacaccaat cgctgcaaca gaccttggtt
      781 ttgaaccagg tatcccagtt atcgaagctg gaccaatctt attccgtatc ccagcaatga
      841 gcgttccagt atttgacaat attagacttc ctgcgaaaca aaatatggta tagtagttcc
      901 atgaattatg aagcaagtaa acaactaact gatgcacgat ttaagcgtct tgttggtgtt
      961 caacgcacga cttttgaaga gatattagct gtattaaaaa cagcttatca acttaaacac
     1021 gcaaaaggtg gacgaaaacc taaattaagc ctagaagact ttcttatggc cactcttcaa
     1081 tatgtgcgag aatatcgaac ttatgaagaa attgcggctg attttggtat tcacgaaagc
     1141 aacttaatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt
     1201 tcaagaactc ctctcagttc tgaggacaca gtaatgattg atgcgacgga agtaaaaatc
     1261 aatcgcccta aaaaaagaat tagcgaatga ttctggtaaa aagaaatttc acgctatgaa
     1321 ggctcaagcg attgtcacaa gtcaagggag aattgtttct ttggatatcg ctgtgaacta
     1381 tggtcatgat atgaagttgt tcaaaatgag ttgcagaaat atcggacaag ctggtaaaat
     1441 cttggctgac agtgattatc aagggctcat gaagatatat cctcaagcac aaactccacg
     1501 taaatccagc aaactcaagc cgctaacagc tgaagataaa gcctgtaacc atgcgctatc
     1561 taaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta aaatgttttc
     1621 aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga gtgctggtat
     1681 tatcaatcat gaactaggat tctagttttg caggaagtct aataaagaaa ctagcaaggt
     1741 caggccttac tttattggtg gacatccagg atttaattgt cctctacttg atgatgaagt
     1801 ctatgaagat tattatctag agtttgagaa agaagagatt tgctctgttc cacgtctttt
     1861 cccagaaaca ggtatgttgg atttccaaga tagaagtcca tggctagagg gacaaaaaga
     1921 aatagatctt agttatgatc ttttcagcac agatgcagtg actttggatg aattgcaatc
     1981 tcgaacaatt gcccttcgtt ctcttaaaca tgataaggga ttgaaagtgc attttgcaga
     2041 atttccaaac ctcattatct ggtcaacttt gaacaaggga tctttcatta cctttgaacc
     2101 atggtctggc ttgtcaacat tccttgaaga aggagatcat ttagaagata agaagaatgt
     2161 ttgtctctta gaagccaatc aggttgaaga attagggttt gagatagaag ttttataaaa
     2221 aagtaaaaaa taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt
     2281 atattatgtt tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa
     2341 tccagcatag aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc
     2401 tgcaggtttg agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct
     2461 tgtggatgtt acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga
     2521 agtaaacaaa gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt
     2581 tatggttgca actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc
     2641 ttatatgact cgtggccaca acaactcacg tatgatcact atgggtgcac aacttgttgg
     2701 tgatgaattg gctaaaaaca tcgctaaagg atttgttaac ggtaaatacg atggtggacg
     2761 tcatcaaatc cgggtcgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg
     2821 taagttaaaa atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc
     2881 ggtttcagaa tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca
     2941 tacacggact cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca
     3001 agctgatctt ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa
     3061 agttccaggt gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga
     3121 acaattgaac gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg
     3181 tgatatcatc gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt
     3241 gattgcgaaa attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt
     3301 tacagaattc cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga
     3361 ttttaacagt cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga
     3421 ttgatactgt caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggactcaatg
     3481 ttacccgagt actttcagaa tttggcgatt ctgttcttgc tactggttta gtgggtggca
     3541 aacttggtga gtttttggtt gaacatatcg ataatcaagt aaagaaagat ttcttctcaa
     3601 ttcagggaga aactcgtaac tgtatcgcta ttctccacgg agacaaccaa acagaagttc
     3661 ttgaaaaagg tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa
     3721 aactcttgga gtcagttgaa gtagtggcca tctcaggtag tctgccagct ggccttccag
     3781 ttgattacta tgcgagcttg gtagaacttg ctaatcaagc tggcaagcct gtagtcttgg
     3841 actgctcagg tgcagcactt caggctgttc ttgaatcacc ccataaacca acagtcatca
     3901 aaccaaataa tgaagaattg tctcagcttc ttggaagaga agtttctgag gatttggatg
     3961 aattaaaaga agtacttcaa gaacctttgt ttgcagggat tgaatggatt atcgtttcac
     4021 ttggtgccaa cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta
     4081 gaattcaggt ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag
     4141 gacttcttca caaagaatcg gatgcagaat tactcatcaa ggcaaatgtc cttggtatgc
     4201 tcaatgctca agaaaaaatg actggtcatg tcaatatggc caactatcaa gctctatatg
     4261 atcaattaat agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta
     4321 gaaaaacttt ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct
     4381 ttgaaacgcc tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa
     4441 cttaaagtct tggtagcaga tgaattgact aaatatgctt catctatgct tcttgaccct
     4501 gagtatggac ttccagcaac taaagctctt gatgaaaaag ctggtcttct ccttgcttat
     4561 gaaaaaacag gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg
     4621 tctgcaaaac gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat
     4681 gtagatagct cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct
     4741 gagtgtgtgg ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt
     4801 gcggatgcag gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggcgctatg
     4861 aaagtctttt cagaaccacg ctttaacatt gatgttttga aagttgaagt tcctgttaac
     4921 attaaatatg ttgaaggctt cgctgaaggt gaagtagttt atacacgtga agaagcagca
     4981 gccttcttca aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt
     5041 gtatcagcta aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt
     5101 aacggagttc tttgtggccg tgctacatgg gcaggatcag ttgaagctta catcaaagat
     5161 ggtgaagcag cagctcgcga atggcttcgc acaactggat ttgaaaacat tgacgaactc
     5221 aacaaagttc ttcaaagaac agcaacttca tggaaagaac gcgtgtaaga aagtcctcct
     5281 agtttaggaa catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat
     5341 aacttacttg tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata
     5401 ttaggcatga tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt
     5461 ttttgtactc atataccttt aagatataaa aggaggttga catgtatcga attctaaatc
     5521 caatgaatca caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg
     5581 gtaaggggat tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga
     5641 aaatctttcg gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg
     5701 ttccgcttga ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc
     5761 attatccgat tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc
     5821 aagctctaac tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc
     5881 ctgtcgcttt tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc
     5941 attttcctga ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa
     6001 atgaagtgga acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag
     6061 aagttttaac ggactatgca attcaacgaa ccaaaaaaaa taatcatttc tatgatcgct
     6121 ttatgattca tttgaattat ttcttggatt atttagacag atctagagat gataaccaat
     6181 cacttctgga tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt
     6241 ccaagatcta tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag
     6301 tttatctagt tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa
     6361 ggagaattct atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta
     6421 tgctggcgat gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt
     6481 tgaaaaagcg gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc
     6541 gcaaacaagt ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat
     6601 gatgcatggc caagaccact tgatgacaac tatcttgtta aaggatttga tgcaccattt
     6661 gatcgaactc tataagagag gagttaaata atgaacaaac taattgcatt tatcgagaaa
     6721 ggaaagcctt tctttgagaa actatctcgt aatatctatc ttcgtgctat tcgtgatggt
     6781 ttcattgcag gtatgcctgt tattctcttc tcaagtatct ttatcttgat tgcctttgta
     6841 ccaaactcat ggggctttaa atggtctgat gaagttgtag cctttctgat gaaaccttat
     6901 agctattcta tgggtattct ggctctcttg gtagctggta caacagctaa gtcattgact
     6961 gactcagtaa accggagcat ggaaaaaacc aatcaaatca actatatgtc aacattgttg
     7021 gcagcaattg ttggtttgtt gatgttggca gctgatccta tcgaaagtgg tctagctact
     7081 ggattcttgg ggacaaaagg tttgctttca gccttccttg ctgccttcgt tactgtagcc
     7141 atctataagg tttgtgttaa gaacaacgtc actattcgta tgcctgacga agttccacca
     7201 aatatctcac aagtctttaa agatgtgatt ccattcactc tctcagttgt ttcactttat
     7261 gctcttgatt tactagctcg tcattttgtt ggtgcaagcg tagctgaatc aatcggtaaa
     7321 ttctttgcac cactcttctc tgcagctgat ggctatcttg gtattaccat tatctttggt
     7381 gcctttgcct tcttctggtt tgtgggtatt catggtccat ctattgttga acctgctatc
     7441 gcagcaatta cctatgcaaa tgctgaagtc aacttgaacc ttctccaaca aggtatgcac
     7501 gctgacaaga ttcttacttc tggtacacaa atgtttatcg ttaccatggg tggtactggt
     7561 gcgacactgg ttgttccatt catgttcatg tggttaacaa aatcaaaacg taaccgtgca
     7621 atcggacgtg cttcagtagt tcctaccttc ttcggtgtaa atgagccaat cttgtttggt
     7681 gcacctcttg ttttgaatcc aatcttcttc attccattta tctttgctcc aattgcaaac
     7741 gtatggattt tcaaattctt tattgaaact cttggaatga actcattcac tgctaatcta
     7801 ccatggacaa ctccagctcc actaggtcta gttcttggaa ctaacttcca agtgctatca
     7861 ttcattcttg ctgcccttct aatcgtggtt gacgttgtca tttactatcc attccttaag
     7921 gtctatgatg aacaaattct tgaagaagaa cgttcaggta agtctaatga tgaattgaaa
     7981 gaaaaagttg ctgcaaactt caacactgca aaagcggatg ctattcttga aaaagcaggt
     8041 gtcgatgcag cacaaaatac catcactgaa gaaacaaatg tcctcgttct ctgtgctggt
     8101 ggaggtacaa gtggactcct tgcaaatgct ttgaataagg cagcagcaaa atacaatgtc
     8161 cctgtgaaag cagcagcagg tggctatggt gctcaccgtg aaatgttgcc agagtttgat
     8221 cttgttatcc ttgcccctca agttgcttca aactttgaag atatgaaggc agaaacagat
     8281 aagctcggta ttaaacttgc taagacagaa ggcgctcaat acatcaaatt aactcgtgat
     8341 ggaaaaggtg ctcttgcatt cgtacaagcg caattcgatt aaggctagag actctgaaat
     8401 agtctcctat cgttacggaa atcgctatgg cgaatttcct attattaatt cgtcggtaaa
     8461 aagatatcgt ttttacctcc tcatgtcaca attcggtgac ttggtacaag aagtgagatg
     8521 gagaaggatg gctcactgac tcctctcctc tcacttttac tttatttaaa tcaagaaata
     8581 ggtgaaaaaa atgacaaaaa cacttccaaa agactttatt tttggtggcg caacagctgc
     8641 ctatcaagca gaaggtgcta cacatactga tggtaaagga ccagtagcgt gggataaata
     8701 tcttgaagat aactactggt acactgcaga accagccagt gatttttaca atcgatatcc
     8761 agttgacctc aagctagcag aagagtatgg tgtcaatggc attcgaattt ctattgcctg
     8821 gtcacgtatt ttcccgactg gttatggtca agtaaatgct aaaggtgttg agttttatca
     8881 taatttattt gcagagtgtc acaaacgtca tgttgagccc tttgtaactc ttcatcactt
     8941 tgacacgcca gaagctctcc actcaaatgg agacttctta aaccgtgaaa atatcgaaca
     9001 ttttgtagac tatgctgcct tctgttttga agaatttcca gaagtaaact attggacaac
     9061 ctttaatgaa attggaccaa tcggtgatgg tcaatatttg gttgggaaat tccctccagg
     9121 tatccagtac gaccttgcca aagtctttca atcacaccac aatatgatgg tgtctcatgc
     9181 atgcgcggta aaattgtaca aagagaaagg ctataaaggg gaaattggtg ttgttcacgc
     9241 cctgcctact aaatatcctc tagatcctga aaatccagca gatgttcgtg cagctgagtt
     9301 ggaagatatc atccacaata aattcatctt agacgcaact tatctaggtc gctattcagc
     9361 tgaaaccatg gaaggtgtca accatatctt atcagtcaat ggtggtagct tggatcttcg
     9421 tgaagaagat tttacagcat tagaagctgc aaaagacttg aatgatttcc taggaatcaa
     9481 ctactatatg agtgactgga tggaagcctt tgatggagaa actgaaatta tccataatgg
     9541 taaaggtgaa aaaggaagct ctaaatacca aatcaaaggt attggtcgtc gtgtagctcc
     9601 tgactatgta ccacgcacgg attgggattg gattatctac cctcaaggtt tgtatgacca
     9661 aatcatgcgc gtgaagaaag attatcctaa ctataagaaa atctacatca ctgaaaatgg
     9721 tcttggatat aaagatgagt tcgttgataa cactgtttac gatgatggac gtattgatta
     9781 cgtgaagcaa cacttggagg ttttatctga tgcgattgca gatggagcta atgtaaaagg
     9841 ttacttcatt tggtcattaa tggatgtctt ctcatggtca aacggttatg agaaacgtta
     9901 tggtctcttc tacgtagatt ttgaaactca agaacgttat cctaagaaat cagctcactg
     9961 gtacaagaaa gtagcggaaa ctcagattat agactagtag aattagtcat tagatataga
    10021 attttagtga gtcaaa
//
LOCUS       DAVMEF010000063         9835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMEF010000063 DAVMEF010000000
VERSION     DAVMEF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.063"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            15..2528
                     /gene="secA"
                     /locus_tag="VYQ30_001155"
     CDS             15..2528
                     /gene="secA"
                     /locus_tag="VYQ30_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1966266.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            2609..3640
                     /locus_tag="VYQ30_001156"
     CDS             2609..3640
                     /locus_tag="VYQ30_001156"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1966267.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            3642..4673
                     /locus_tag="VYQ30_001157"
     CDS             3642..4673
                     /locus_tag="VYQ30_001157"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1966268.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            4712..5080
                     /locus_tag="VYQ30_001158"
     CDS             4712..5080
                     /locus_tag="VYQ30_001158"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1966269.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            5070..6173
                     /locus_tag="VYQ30_001159"
     CDS             5070..6173
                     /locus_tag="VYQ30_001159"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1966270.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDAYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            6192..8207
                     /gene="recG"
                     /locus_tag="VYQ30_001160"
     CDS             6192..8207
                     /gene="recG"
                     /locus_tag="VYQ30_001160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1966271.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFILAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            8235..8393
                     /locus_tag="VYQ30_001161"
     CDS             8235..8393
                     /locus_tag="VYQ30_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966272.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHCFEVADRTDEVS
                     NIYTVRRR"
     gene            8719..9699
                     /locus_tag="VYQ30_001162"
     CDS             8719..9699
                     /locus_tag="VYQ30_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1966273.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFRIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAVYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         2867 a         1944 c         2375 g         2649 t
ORIGIN      
        1 aagtaaggaa aaacatggct aatattttaa aaacaattat cgaaaatgat aaaggagaaa
       61 tccgtcgtct ggaaaagatg gctgacaagg ttttcaaata cgaagaccaa atggctgctt
      121 tgactgacga ccaactaaaa gcaaaaacag ttgaatttaa ggaacgttat caaaatggag
      181 aatcactgga ttcattgctt tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg
      241 tcctaggtct cttcccttat aaggttcagg tcatgggggg gattgttctt caccatggtg
      301 acgtgccaga gatgcgtaca ggggaaggga aaaccttgac tgcgaccatg ccggtatacc
      361 tcaatgccct ttcaggtaaa ggggttcacg tagttacggt taatgaatac ctgtcagaac
      421 gtgacgcgac tgagatgggt gaattgtact cttggcttgg tttgtcagta gggattaact
      481 tggctaccaa atctccaatg gagaaaaaag aagcctatga gtgtgatatt acttactcaa
      541 ctaactcaga aatcggattt gactaccttc gtgacaatat ggtcgttcgc gctgaaaaca
      601 tggtacaacg tccgcttaac tatgccttgg tcgatgaggt tgactctatc ttgattgacg
      661 aggctcgtac acctttgatt gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc
      721 acatggcaga ccactatgta aaatctttga acaaagatga ctacatcatc gatgtgcagt
      781 ctaagactat tggtttgtct gattcaggga ttgacagggc tgaaagctac ttcaaacttg
      841 aaaacctcta tgacatcgaa aacgtggctt tgacccactt tatcgataac gcccttcgtg
      901 ccaactacat catgcttctc gatattgact atgtggtgag cgaagagcaa gaaatcttga
      961 ttgtcgacca atttacaggt cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc
     1021 aagctattga agccaaagaa ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa
     1081 tcacgtacca aaacctcttc cgtatgtaca aaaaattgtc tggtatgacg ggtacaggta
     1141 agactgagga agaagaattt cgtgaaatct acaacattcg tgttattcca atcccaacaa
     1201 accgtcctgt tcaacgtatt gaccactcag accttcttta tgcaagtatc gaatctaagt
     1261 ttaaagcggt tgtcgaagac gttaaggctc gttaccaaaa gggtcaacct gtcttggttg
     1321 gtacagtagc ggttgaaact agtgactaca tttctaagaa attggttgca gctggtgttc
     1381 ctcacgaagt cttgaatgcc aaaaaccact atagagaagc ccaaatcatc atgaatgctg
     1441 gtcaacgtgg tgccgttacc atcgcaacca acatggcggg tcgtggtacc gacatcaagc
     1501 ttggtgaagg tgttcgtgaa cttggaggac tttgtgttat tggtacagaa cgtcatgaaa
     1561 gtcgtcgtat cgataaccag cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt
     1621 cacaattcta cctatctctt gaagatgatt tgatgaaacg ttttggttct gaacgcttga
     1681 agggaatctt tgaacgcttg aacatgtctg aagaggcgat tgagtctcgc atgttgacgc
     1741 gtcaggttga agcagctcag aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag
     1801 tccttcaata cgatgatgtc atgcgtgaac aacgtgagat tatctatgct caacgttacg
     1861 atgtcatcac tgcagatcgt gacttggcac ctgaaattca gtctatgatc aaacgcacga
     1921 ttgaacgtgt cgttgatggt catgcgcgtg ccaaacaaga tgaaaaacta gaggcaattt
     1981 tgaactttgc taagtacaac ttgcttcctg aagattctat tacgatggaa gacttgtcag
     2041 gcttgtctga taaggccatc aaggaagagc ttttccaacg tgccttgaag gtttacgata
     2101 gtcaggtttc aaaactacgc gatgaagaag cagttaaaga attccaaaaa gttttgattc
     2161 tacgagtggt ggataacaag tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg
     2221 cggttggact tcgtggctat gctcagaaca accctgttgt tgagtatcag gcagaaggtt
     2281 tccgtatgtt taatgatatg attggttcga ttgagtttga tgtgacacgc ttgatgatga
     2341 aagcacaaat tcatgaacaa gaaagaccac aggcagaacg tcatatcagt acaacagcga
     2401 ctcgcaatat cgctgctcac caagcaagta tgccagaaga tttggatttg agccagattg
     2461 gacgcaatga actttgccca tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa
     2521 gacaataaaa tgagatagtt tagaggcgga tatcttgtga aaagtaaatt tttactgggt
     2581 atccgtttgc tttataagga gatgagttat ggtatttaca gcaaaaagct ctaaaataaa
     2641 tatagaagaa gttcgtgcct tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca
     2701 gcgagatcaa gagctagaag ccattatacg tggagaagac cagcgaattc tcttggtaat
     2761 cgggccatgc tcatctgaca acgaagaagc tgtccttgaa tacgctaagc gtttggcagt
     2821 cctacaagaa gaagtggcag atcgtatctt tatggttatg cgtgtttata ctgccaaacc
     2881 ccgtaccaac ggagatggct ataagggctt gattcaccag cctaacgcga cagaagcgcc
     2941 tagtcttatc aatggaatca aagccgttcg ccatcttcac tatcgtgtca tcacagaaac
     3001 agggatgaca actgctgatg aaatgcttta tcctgaaaac cttccgcttg tagatgattt
     3061 gatttcttac atggcagttg gtgcccgttc agttgaagac cagcaacacc gctttgtggc
     3121 aagtggggca ggattttcta ctggttttaa aaatccaacc tctggaaatc tcaatgtcat
     3181 gtttaatggg atttatgctg ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt
     3241 agaaacaact gggaacccgc tttcacacgc tattcttcgt ggtgctctta atgagtatgg
     3301 aaaaaatatt cccaactact attatgacaa tttaattgat accattgccc agtatgagaa
     3361 aatgggcttg gaaaatcctt ttatcatcat tgataccaat catgacaatt ctggtaagca
     3421 gtatattgaa cagatccgaa ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa
     3481 aattaagcag ttcgttcgtg gttttatgat tgagtcttat ctggaagatg gtcgacaaaa
     3541 tgagccagaa gtatttggta agtctatcac agacccttgc ctgggttggg ataacacaga
     3601 agctcttgtc agagaaattt acaaaacgtt aggagaataa gatggcattt attgaaaaag
     3661 gtcaagaaat cgatatggaa gtcatcaagg ctgaaaccca attgtctgcg gaagccttga
     3721 gactcaagga aagccgtgac agggaattgg cagatattat ttcaggggaa gatgaccgta
     3781 ttctcttggt gattggtcct tgctcttctg ataatgaaga ggcggtcttg gaatatgctc
     3841 gccgtttatc cgccttgcaa aagaaggtag cggataagat tttcatggtc atgcgcgtgt
     3901 atactgctaa gcctcgtacc aatggagacg gctataaagg attagttcac cagccagata
     3961 cttctaaggc tccaagcctg attaatggct tgcaggctgt gcgccagttg cactaccgcg
     4021 tgattacaga gactggtttg acaacggcag atgagatgct ttatccgtca aatctgatct
     4081 tggtggatga cttggtcagc taccatgccg ttggagctcg ttctgtggaa gaccaagagc
     4141 accgctttgt ggcttctggg attgatgcac cagtagggat gaaaaatcca acctcaggaa
     4201 atttgggtgt tatgtttaac gccatctatg ctgctcaaaa caagcaaacc ttcctttatc
     4261 atgggcagga agttgagaca tcaggtaatc ctttggccca tgttatcctc cgtggagcag
     4321 tcaacgagta tggcaattat atgccgaatt actactatga aaacctactc caagccattg
     4381 aacgctatga aaccatggga cttgaaaatc cttttatcct cattgacacc aaccatgata
     4441 actcaggcaa gcaatatatg gagcagattc gaattgttcg ccagaccttg cagaatcgtg
     4501 attggaatga gaaaattaaa aagacggttc gaggatttat gattgaatct tacctagcag
     4561 atggtcgtca aaaccaacca gagatctttg gttgctctat tactgaccct tgcctaggtt
     4621 gggaaaatac agaggccttg gtagaagaga tttatgttac cttgacaaaa taagtgaaaa
     4681 ggatggagtt ggggaatctc aactcctttt gatgagaatg atagttggac acggaattga
     4741 catcgaagaa ttggcttcga tagaaagcgc agttacacga catgaaggat ttgctaagcg
     4801 tgtactgacc gctcaggaaa tggagcgctt caccagtctc aaaggacgca ggcaaataga
     4861 atatttagct ggtcgctggt cggctaagga ggccttttcc aaggctatgg gaacgggcat
     4921 tagcaagctc ggttttcagg atttggaagt cttgaacaat gaacgtgggg cgccttattt
     4981 tagtcaggca ccattttcag gaaagatttg gctgtctatc agccacaccg atcagtttgt
     5041 gacagccagt gtcattttgg aggaaaatca tgaaagctag tccacataga ccaaccaagg
     5101 ctctgattca tctgggagct attcgacaaa atattcagca aatgggggct catatccctc
     5161 aaggaacgct caagttggct gtggtcaagg ccaatgctta tggtcatgga gctgttgccg
     5221 ttgccaaggc aattcaagat gatgttgatg gcttttgcgt ttccaatatc gatgaagcca
     5281 ttgaactcag acaagctgga ctcagcaagc caatcctcat tttaggagtt tctgaaatcg
     5341 aagctgttgc tctagctaaa gaatatgact tcaccttgac agtggctgga ctggagtgga
     5401 ttcaagcact cttagataag gaagtggacc taactggatt gacagtccac ctcaagattg
     5461 attcagggat gggacggatt ggttttagag aggcaagtga ggttgagcag gctcaagatt
     5521 tgctacaaca acacggtgtt tgtgttgaag gaatctttac ccactttgct actgctgatg
     5581 aggaatcaga tgcctatttt aatgcccagt tagaacggtt taaaactatt ttagctagta
     5641 tgaaggaagt tccagagctg gttcatgcta gcaattctgc aacgactctt tggcatgtag
     5701 agactatttt caatgcggtt cgtatgggag atgccatgta tggtctcaat ccaagtggag
     5761 cggtcttgga tttgccttat gatttgatac cggccttgac cttggagtct gctctggttc
     5821 atgtcaagac agttccagct ggagcttgca tgggctatgg agcaacttat caagcggata
     5881 gcgagcaagt catcgcaacc gtgccaatcg ggtatgcaga tggatggaca agagacatgc
     5941 aaaatttctc tgtcttggta gatggccaag cttgcccaat tgtcggcagg gtttcgatgg
     6001 accaaatcac tattcgattg cctaagcttt atccgctagg aaccaaggta actttgattg
     6061 gctccaatgg ggataaggaa atcactgcaa ctcaggtagc gacctaccgc gtcaccatta
     6121 actatgaggt ggtttgcctc ctcagcgacc gtattccgag agaatattat tagaaaagaa
     6181 aggagtggag catgaatcta catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt
     6241 cagcagaaaa atacgccaaa ctaggaattg aaaacttgca agatctcttg ctctactttc
     6301 ctttccgtta tgaagacttc aaaaccaagc aggtgctgga actagaagac ggtgagaagg
     6361 cagttctttc tggtcaggta gtgactcctg ctagtgtcca gtattatggt ttcaagcgca
     6421 atcgcctgcg ttttagtctc aagcagggag aggtcgtttt tgcggtgaat ttctttaacc
     6481 agccctatct ggctgataaa atagagttgg gagcaactct tgctgtcttt ggaaaatggg
     6541 accgcgctaa ggctagtctg actgggatga aggttctggc tcaggtagaa gatgacctcc
     6601 agcctgtcta tcgtctggct cagggaatca gtcaggccag tctggttaag gtcatcaaga
     6661 cggcttttga tcagggacta gacctcttga tagaagaaaa tctgccccag tctttactag
     6721 acaaatacaa actcatgtcc cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt
     6781 tggcagaata caagcaggct cttcgccgta taaagtttga ggaactcttt tatttccaaa
     6841 tgcagctgca gatgctcaag tctgaaaata gagttcaggg aagtggtctg gttctgaatt
     6901 ggtctcagga aaaagtgaca gcagttaaag taagtcttcc ttttgccctg acccaagctc
     6961 aggaaaagag tttgcaggaa attttaactg atatgaagtc cgaccaccac atgaatcgtc
     7021 tcctacaagg ggatgtgggg agtggaaaaa cggtagtcgc tggcttggcc atgtttgcgg
     7081 cagtgacagc aggttatcag gctgccctaa tggtaccaac agaaatcctc gcagagcaac
     7141 actttgagag tttacagaac ctttttccca atttgaaact ggctctcttg acaggttcct
     7201 tgaaagctgc agaaaagaga gaggtcttgg agaccattgc caagggtgag gccgatttga
     7261 ttatcggaac ccacgctctg atacaagatg gggtggagta tgctcgtctt ggtttgatta
     7321 ttatcgatga gcagcaccgt tttggtgtgg ggcaaaggcg tattttacgg gaaaaaggcg
     7381 acaatccaga tgtcctcatg atgacggcga ctcccattcc acggacgctt gccatcacag
     7441 cctttggaga tatggatgtt tccattatcg accagatgcc agcaggtcgg aagcctattg
     7501 tgacgcgctg gatcaaacat gagcaactac ctcaggtctt gacttggtta gagggggaaa
     7561 ttcaaaaagg ttcccaagcc tatgtcatct ctcctttgat tgaagaatca gaagctctag
     7621 atttgaaaaa tgccattgcc ttatcagagg agttgacgac tcattttgca ggcaaggcag
     7681 aggtggctct tctacatggt aggatgaaga gtgacgaaaa agaccagatc atgcaggatt
     7741 tcaaggagag aaagacggat attctggttt cgacgacggt tattgaggtt ggggtcaacg
     7801 ttcccaatgc gactgtcatg attatcatgg atgccgatcg cttcggtctc agtcaacttc
     7861 accagcttag aggtcgtgtc ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcca
     7921 atcccaagac ggattctggg aaagaccgca tgcgcatcat gacagaaacg accaatggat
     7981 ttatccttgc ggaggaagat ttgaaaatgc gtggttctgg tgagattttt ggaaccagac
     8041 agtcaggact tccagagttc caagtggctg atattatcga agattttccg attttagaag
     8101 aagcaagaaa ggttgctagc tacattagtt ctatagaagc ttggcaagaa gatccagagt
     8161 ggcgcatgat tgcccttcat ctggaaaaga aagaacatct ggattaagct ttctctaagg
     8221 aaaacttata ctcaatgaaa atcaaagagc aaactaggaa gctaaccgca ggttgctcaa
     8281 aacactgttt tgaggttgta gatgaaactg acgaagtcag ctcaaaacac tgttttgagg
     8341 tggcagatag aactgacgaa gtcagtaaca tatatacggt aaggcgacgc tgacgtggtt
     8401 tgaagagatt ttcgaagagt attaagctag tttttaggtt tggctcttat actagagtca
     8461 tcaaaaagaa acgaggactc tcatatgaca gtaacgatta aagtaaatta ccaaaccact
     8521 ttccaaaaga aggaagcaaa aaactagtat aaacagaaga gagagcgaaa tgctcttttt
     8581 cgtttctaaa actactttca gcccatcatc ctaaaagtaa agaatctaaa ttcactttct
     8641 atttatcctt ctttcttgca ttgattacat agatatgcta cagttgtggt aacgattaca
     8701 aaataaaagg agcatgctat gaaaaatcca gctttgctag aagaaattaa gacctataga
     8761 ggaagggatg aggttccgga agactttgat gctttctggg atggggaagt gaaaaatgtt
     8821 tccacgcttc catcctacca cttggaggaa agagatttcc gcattcctca agtcaagtgc
     8881 tatgagttaa catttgaagg aagcaaggaa ggaaaggtct atgcacgcat tgttcttcca
     8941 aagagtgagg agaaggttcc actaatcttc cattttcatg gttatatggg acgtggctgg
     9001 gactgggccg acatgctggg cttcaccgta gctggttacg gtgttgtttc catggatgtg
     9061 cggggccagt caggttactc acaagacggc ttgcgttctc ctttaggaaa taccgtgaag
     9121 gggcatatta tccgtggtgc tgtggaaggt cgggaccacc tcttttataa ggatgtttat
     9181 ctggatattt accagttggt cgaaattgtt gctagtctgt ctcaggttga tgagaagcgt
     9241 ctttctagct atggtgcctc acaaggaggg gctctagccc tagttgcagc agcgctcaat
     9301 cctcgaattc agaaaacagt tgctgtttat cccttcttgt cagacttcag acgggtgatt
     9361 gagattggta atactagcga ggcttacgac gaacttttcc gttatttcaa gtttcacgac
     9421 cccttccatg aaacagagga ggaaatcatg gcgacccttg cctatatcga tgtcaaaaat
     9481 cttgcccatc gtatccaagg tgaggttaag atgattacgg gcttggacga cgatgtttgc
     9541 tatcccatta cccagtttgc gatttataat cgtctgacct gcgataaaac ctatcgcatc
     9601 atgcctgagt atgctcacga agccatgaat gtatttgtca atgaccaagt ctacaactgg
     9661 ctctgtggaa gtgagattcc ttttaaatat ctaaaataag gagtcgactc taagcacaaa
     9721 atcttaaaaa ttacaaacac gcatagtatc aggggattaa aaaaacttga tactatgcgt
     9781 tttatcatgg aaatatagta aaatgaaaca agaataggac aaaacgatca ggaca
//
LOCUS       DAVMEF010000064         9778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMEF010000064 DAVMEF010000000
VERSION     DAVMEF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.064"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..182
                     /locus_tag="VYQ30_001163"
                     /pseudo
     CDS             1..182
                     /locus_tag="VYQ30_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            265..648
                     /locus_tag="VYQ30_001164"
     CDS             265..648
                     /locus_tag="VYQ30_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1966274.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            700..1389
                     /locus_tag="VYQ30_001165"
     CDS             700..1389
                     /locus_tag="VYQ30_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966275.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     FLPSILFALPHFLSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1431..1679
                     /locus_tag="VYQ30_001166"
     CDS             1431..1679
                     /locus_tag="VYQ30_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966276.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFLGAGYVVGFWLLKWFVLERLELKDDA"
     gene            1709..2320
                     /locus_tag="VYQ30_001167"
     CDS             1709..2320
                     /locus_tag="VYQ30_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966277.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGAGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2482..3276
                     /locus_tag="VYQ30_001168"
     CDS             2482..3276
                     /locus_tag="VYQ30_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1966278.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3273..3908
                     /gene="trmB"
                     /locus_tag="VYQ30_001169"
     CDS             3273..3908
                     /gene="trmB"
                     /locus_tag="VYQ30_001169"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1966279.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPVHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4034..4513
                     /gene="rimP"
                     /locus_tag="VYQ30_001170"
     CDS             4034..4513
                     /gene="rimP"
                     /locus_tag="VYQ30_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1966280.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4557..5693
                     /gene="nusA"
                     /locus_tag="VYQ30_001171"
     CDS             4557..5693
                     /gene="nusA"
                     /locus_tag="VYQ30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1966281.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5715..6008
                     /locus_tag="VYQ30_001172"
     CDS             5715..6008
                     /locus_tag="VYQ30_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1966282.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKERQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6001..6300
                     /locus_tag="VYQ30_001173"
     CDS             6001..6300
                     /locus_tag="VYQ30_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1966283.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6317..9109
                     /gene="infB"
                     /locus_tag="VYQ30_001174"
     CDS             6317..9109
                     /gene="infB"
                     /locus_tag="VYQ30_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1966284.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKSNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRDERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9360..9710
                     /gene="rbfA"
                     /locus_tag="VYQ30_001175"
     CDS             9360..9710
                     /gene="rbfA"
                     /locus_tag="VYQ30_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1966285.1"
                     /translation="MVNHFRIDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2961 a         1802 c         2243 g         2772 t
ORIGIN      
        1 gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat gtttgtcatt
       61 tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc ttatataggt
      121 gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat tataaaaagt
      181 agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa ttgttagtga
      241 aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat tttcttgagt
      301 agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc tattgggtgg
      361 cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa taaaggggtg
      421 gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt tggaaaaacc
      481 ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct tggtttggat
      541 gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt aatgtatcta
      601 ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc ttacagcagt
      661 agagcctgac aaggtgaggt catagtaggg aggattttta tgaaaaagta tcaacttcta
      721 ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttggtctttc tcagctgacg
      781 cttatcgtcc aaaactattg gcaattttct tctcagatag gcaatttatt ctggattcaa
      841 aatatcttga gtttactttt tattggagtc atgattgtgg ttcttgttaa gacaggccat
      901 ggttatctct tccgcattcc aagaaaaaaa tggctttggt attcgatttt gacagtatta
      961 gtggtagtgt tccagatctc ttttaacgtt cagacagcta aacatgttca gtcaactgcg
     1021 gaaggttggg ctgtattgat tggttatagt gggactaact ttgcagagct aggtatttat
     1081 atagccctgt tctttctggt tccactgatg gaagaattga tttatagagg attactgcaa
     1141 catgctttct ttaagcattc gcgatttggt cttgatttgt ttcttccgtc cattttattt
     1201 gctctccctc attttttgag tctgcctagt ctgttagata tcttcgtctt tgcaacagtt
     1261 ggaatcatct ttgctggttt gacccgctat accaagagca tttatccatc ctatgcggtg
     1321 catgtgatca ataatattgt agcgaccttc ccgtttttgc tcacttttct acatagggtc
     1381 ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc atgtataaac
     1441 acttattttt cctagattcc aaaactttag atcggttgac accctatatt ctagtcttgg
     1501 cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg gtggttttta
     1561 atttcctaaa ttccatgcta gctttaatgg ctatattctt aggggctggc tatgtggtcg
     1621 gattttggct actaaaatgg tttgttttgg aaaggttaga gctaaaggat gacgcgtagg
     1681 aagtctgttt ggtgaggagg ataattttat ggagtttttt gataaatttc atgccttgtg
     1741 ctttggattt ttagtactaa taatcgtcat tacagttcct tatacgatta accatgggga
     1801 tttttttcaa aatgaatctg cattgattat tgtaagtctt ctagtaacct ctctgagtgt
     1861 tgcttatgct agaaagtttg aaatgatttc ttttgggatg ttaagcaaga aacaactttt
     1921 gcttttcatt gcaatctttc ttctaagtgt gcttgagacg ctggtttata ttcatttctt
     1981 cgctgtttct tctggcgcag gagttcaaca cttggcggaa gtcagcagag gaatttccct
     2041 gtctttgatt ttgactacct cagtttttgg gccaattcag gaggaactca ttttcagagg
     2101 acttcttcaa ggtgcggttt ttgacaattc ttggttaggg cttgtgctaa cttcctctct
     2161 cttttctttc atgcatggac cttctaatgt cccttcgttt attttttatc tacttggggg
     2221 tttgttgctg ggctttgctt ataaaaagag ccaaaaccta tgggtttcta ctctagtcca
     2281 tatgctttac aacagttggc cactcttata ttatttataa aaatcatgaa aaggtaagta
     2341 ggagattgtg cttacctttt tctttctata atgataaccc aaaccactcc agaggctttt
     2401 ctttgagaat cgggttagag tggttgacga ataggccaaa aactagtaga atagtaagga
     2461 aactttatac ggaggaaaga aatggatttg ggtgataatg agctaacact gactcccata
     2521 cctgggaaaa gtggcaaggc ttatatgggt agctatcctg atgggaagcg catctttgta
     2581 aaaatgaaca cctctccaat cctacctggt ctagctagag aacaaattgc tccacaatta
     2641 ttatggagtc gtcgtttggc agatgggcgt gatatgtgtg ctcaagaatg gttgacaggc
     2701 aagatattga ccccctatga tatgaatcgt aagcaaatcg tcaatatttt aacccgtctt
     2761 catcgctcac gtccgttgat gacacaattg agtcgtttgg gctatgccat ggaaacacct
     2821 gtagatttac tacagtcttg gcaggaaacg gctccagatg ctttgcgtaa aaatcatttt
     2881 atcagtgaag tgatggctga tttacgtcag actattccag gatttagaga ggaccatgcg
     2941 accattgtcc atggagatgt acgacatagt aattggattg agacagatag tggcttgatt
     3001 tatttagtag attgggattc ggttcgcttg accgatcgca tgtttgatgt ggcccatatg
     3061 ctctgccatt atatttcaga acatcagtgg aaggaatggt tgacctacta cggttacaag
     3121 tacaatcaaa cggtattaag taaattgtat tggtatggtc aattgtctta tttgagtcag
     3181 atttccaagt attatatgaa ccaagattta gaaaatgtca atcgggagat tcatggtttg
     3241 cgtcatttcc gagacaagta tggaaagaga agatgagagt tagaaatcgt aaaggggcaa
     3301 cagaattact agaggcaaat ccccagtatg tggtcctcaa tcccttggaa gccaaggcaa
     3361 aatggcggga cttgtttggc aatgataatc ccgttcatgt ggaagttgga agtggaaagg
     3421 gtgcctttgt ttcaggtatg gccaagcaaa accctgacat caactatatc gggattgata
     3481 ttcaaaagtc tgttttgagc tacgctttgg acaaggtgct tgaagttgga gtgcctaaca
     3541 tcaagctctt gtgggtagat ggttctgact tgactgacta ctttgaagac ggtgagattg
     3601 atcgcttgta tctgaacttt tcagatccat ggccgaaaaa acgccatgaa aagcgtcgtt
     3661 tgacctacaa gacctttttg gacaccttca aacgtatctt gcctgaaaat ggagaaattc
     3721 atttcaagac ggataaccgt ggcttgtttg agtacagttt agtgagcttt tctcaatatg
     3781 gcatgaaact caatggtgtc tggctagatt tgcatgccag tgattttgaa ggcaatgtca
     3841 tgacagaata cgagcaaaaa ttctcaaaca aggggcaagt tatctaccga gttgaggcag
     3901 aattttaaga gataacctaa aattaggctg tacaagtgct tttgctttac ataagttggc
     3961 aaacgtgcta tactgatagt aagaatatga aaagtgaggc ggggaaatat cttcgcctct
     4021 tgcttatgag gaggtggacg caatcgcaac aatcgtagaa ttagtcagag aagttgtaga
     4081 acctgtcata gaagctcctt ttgaactcgt ggatatcgag tatggaaaga ttggcagtga
     4141 catgattctc agtatttttg tagataaacc cgaaggaatt accttgaacg acacggcaga
     4201 cttgacagaa attatcagtc ctgtcctaga caccatcaag ccagatccct tcccagaaca
     4261 atatttccta gaaattacca gtccaggttt ggaacgtcct ttgaaaacca aggatgccgt
     4321 cgctggagcg gttggaaaat acatccatgt cgggctctac caagccatcg ataagcaaaa
     4381 ggtctttgaa ggaaccttgt tggccttcga agaggacgag ttgactatgg aatatatgga
     4441 caagacgcgt aagaaaaccg tccaaattcc atacagttta gtatcaaaag cacgtttagc
     4501 agttaaatta tagaaaaaga aaggatagct tttgaggatt caaaagtgaa gaaaacatga
     4561 gtaaagaaat gctagaggcc ttccgcattt tggaagaaga caagggaatc aaaaaagaag
     4621 atatcatcga cgcagtagta gagtcgcttc gttccgctta tcgcagacgc tatggtcagt
     4681 cagacagcgt agctattgac ttcaacgaaa aaacaggtga ctttacagtt tatactgtcc
     4741 gtgaagttgt tgatgaagta tttgatagcc gtttggaaat cagcttgaaa gatgctcttg
     4801 ccattaattc agcttatgaa cttggagaca aaatcaagtt tgaagaagca ccagctgagt
     4861 ttggtcgtgt agcagcccaa tctgccaaac aaaccatcat ggaaaaaatg cgcaagcaaa
     4921 cacgtgccat cacttacaat acttacaaag aacatgagca agaaatcatg tctggtacag
     4981 tagaacgctt tgacaaccgc tttatctatg tcaaccttgg tagcatcgaa gcccaattgt
     5041 caaaacaaga ccaaattcct ggagaagttt ttgcttctca tgatcgtatc gaagtttatg
     5101 tttacaaggt tgaagacaac cctcgtggtg tgaacgtctt tgttagccgt agtcatccag
     5161 aaatgatcaa acgtttaatg gagcaagaaa ttccagaagt ttatgatgga actgttgaaa
     5221 tcatgagcgt ggctcgtgaa gcaggtgacc gtacgaaggt tgctgttcgt agccacaatc
     5281 caaacgtgga tgctatcggt acaatcgttg gacgtggtgg tgctaatatc aagaagatta
     5341 ctagcaaatt ccacccagct cgttacgatg ctaaaaatga ccgcatggta ccaatcgaag
     5401 aaaatatcga tgttatcgag tgggtagcag atccagctga atttatctac aatgccatcg
     5461 ctcctgctga ggttgaccaa gttatctttg atgaaaacga cagcaaacgt gccttggtgg
     5521 ttgttccaga taacaagctt tctcttgcca ttggtcgtcg tggacaaaac gtgcgcttgg
     5581 cggctcactt gactggttac cgtatcgata tcaagtctgc tagcgaattt gaagccatgg
     5641 aagacgctgc ttcagtagag ttggaagtag aaaacgatac tgtagaagaa taaaagctgc
     5701 tagaggaggg aaagatgaaa acaagaaaaa tccctttgcg caagtctgtt gtgtctaacg
     5761 aagtgattga taagcgtgat ttgctccgca ttgtcaagaa caaggaaaga caagtcttta
     5821 ttgatcctac gggcaaggcc aatggccgcg gcgcttatat caaactagac aatgcagaag
     5881 ccctagaggc gaaaaagaag aaggtcttta accgcagctt tagcatggaa gtggaagaaa
     5941 gcttttatga cgagttgatc gcttatgtgg atcacaaagt gaaaagaaga gagttgggac
     6001 ttgaataagc aaaagataag taatctcttg gggcttgctc agcgagcagg gcgcatcata
     6061 tcgggtgaag aattggtggt caaggccatt caagacggca aggccaagtt ggtctttcta
     6121 gctcatgatg ctggacccaa tctaaccaag aagattcaag ataaaagtca ttattatcaa
     6181 gtagaaattg taaccgtgtt ttcaacactg gaattaagca tagcagtcgg gaaatcgaga
     6241 aaggttttgg ctgtaacaga tgctggattt acaaagaaaa tgaggtctct tatggaatag
     6301 aagaggagga catgatttgt ctaagaaaag attgtacgaa atcgcaaaag aacttggaaa
     6361 agaaagtaaa gaagttgtag cgcgtgcaaa agagttaggc ttggatgtga aaagccactc
     6421 atcaagtgtg gaagaagctg tcgctgcaaa aattgctgcc agctttaagc ctgcagctgc
     6481 tccgaaagta gaagcaaaac ctgcagcacc aaaagtaagt gcagaaaaga aagccgaaaa
     6541 atctgagcca gctaaaccag ctgtagctaa ggaagaggca aaacctgctg agccagttgc
     6601 tccgaaaaca gaaaaagtag cagcgaaacc gcaaagtcgt aatttcaagg ctgagcgtga
     6661 agcccgtgct aaagagcagg cagaacgacg caagcaaaat aagagcaata accgtgacca
     6721 acaacaaaac ggaaaccgtc agaaaaacga cggccgtaat ggtggaaaac aaggtcaaag
     6781 caaccgcgac aatcgtcgct ttaatgacca agctaagaag cagcaaggtc agcaaaaacg
     6841 tagagatgag cgccgtcagc aagaggacaa acgttcaaat caagcggctc cacgtattga
     6901 ctttaaagcc cgtgcagcag ccctaaaagc agagcaaaat gcagagtacg ctcgttcaag
     6961 tgaggaacgc ttcaagcagt atcaggctgc taaagaagcc ttggctcaag ctaacaaacg
     7021 caaggaacca gaggaaatct ttgaagaagc ggctaagtta gctgaacaag cacagcaagt
     7081 tcaagcagtg gttgaagtcg tccctgagaa aaaagaacct gcagtggata cacgtcgtaa
     7141 aaaacaagct cgaccagaca aaaatcgtga cgattatgat catgaagaag atggtcctag
     7201 aaaacaacaa aagaatcgaa gtagtcaaaa tcaagtgaga aatcaaaaga atagtaactg
     7261 gaataacaac aaaaagaaca aaaaaggcaa taacaagaac aaccgtaatc agactccaaa
     7321 acctgttacg gagcgtaaat tccatgaatt gccaacagaa tttgaatata cagatggtat
     7381 gaccgttgcg gaaatcgcaa aacgtatcaa acgtgaacca gctgaaatcg ttaagaaact
     7441 ttttatgatg ggtgtcatgg ccacacaaaa ccaatccttg gatggggaaa caattgaact
     7501 cctcatggtg gattacggta tcgaagccaa acaaaaggtt gaagtggata atgctgacat
     7561 cgaacgtttc tttgtcgaag atggttatct caatgaagat gaattggttg agcgtccacc
     7621 agttgttact atcatgggac acgttgacca cggtaaaaca acccttttgg atactcttcg
     7681 taactcacgt gttgcgacag gtgaagcagg tggtattact cagcatatcg gtgcctacca
     7741 aatcgtggaa aatggtaaga agattacctt ccttgataca ccaggacacg cggcctttac
     7801 atcaatgcgt gcgcgtggtg cttctgttac cgatattacg atcttggtcg tagcggcaga
     7861 tgacggggtt atgcctcaga ctattgaagc catcaaccac tcaaaagcag ctaacgttcc
     7921 aatcatcgta gctattaaca agattgataa accaggtgct aacccagaac gcgttatcgg
     7981 tgaattggca gagcatggtg tgatgtcaac cgcttggggt ggagattctg aatttgttga
     8041 aatctcagct aaattcaacc aaaatatcga agaattgttg gaaacagtcc ttcttgtagc
     8101 tgaaatccaa gaactcaagg cagacccaac agtgcgtgca atcggtacgg ttatcgaagc
     8161 gcgcttggat aaaggaaaag gtgcggtcgc aacccttctt gtacaacaag gtaccttgaa
     8221 tgttcaagac ccaatcgttg tcggaaatac cttcggtcgt gtccgtgcta tgaccaacga
     8281 ccttggtcgt cgtgttaaag ttgctggacc atcaacacca gtctctatca caggtttgaa
     8341 cgaagcaccg atggcgggtg accactttgc cgtttacgag gatgaaaaat ctgcgcgtgc
     8401 agcaggtgaa gagcgtgcca aacgtgccct catgaaacaa cgtcaagcta cccaacgtgt
     8461 tagccttgaa aacctctttg atacccttaa agctggggaa ctcaaatctg ttaatgttat
     8521 catcaaggct gatgtacaag gttctgttga agccctttct gcctcacttc aaaagattga
     8581 cgtggaaggt gtcaaagtga ctatcgtcca ctcagcggtc ggtgctatca acgaatcaga
     8641 cgtgaccctt gccgaagctt caaatgcctt tatcgttggt ttcaacgtac gccctacacc
     8701 acaagctcgt caacaagcag aagctgacga tgtggaaatc cgtcttcaca gcattatcta
     8761 caaggttatc gaagagatgg aagaagctat gaaagggatg cttgatccag aatttgaaga
     8821 aaaagttatt ggtgaagcgg ttatccgtga aaccttcaag gtgtctaaag tgggaactat
     8881 cggtggattt atggttatca acggtaaggt tgcccgtgac tctaaagtcc gtgttatccg
     8941 tgatggtgtc gttatctatg atggtgaact cgcaagcttg aaacactata aagacgacgt
     9001 taaggaagtt acaaacggcc gtgaaggtgg attgatgatc gacggctaca atgatattaa
     9061 gatggatgat gtgattgagg cgtatgtcat ggaagaaatc aagagataag attttttgct
     9121 cctttcttag gtggtgaggg acgcaagcaa accgatggtt tcattgctta tttttgagcc
     9181 tagggtctca aaaatcccct gtgatgggac tgataaatca gttccatcac tttcaccacg
     9241 gcgaaagaag cagatgactt caaattgaac ttcgtttcaa tttaaactga aaatcaagaa
     9301 gtttaaaata gctaggtctg ctggcctagc ttttggttca aagtagagaa aggaatatca
     9361 tggtaaatca tttccgtata gatcgtgtgg gcatggaaat caagcgtgaa gtcaatgaga
     9421 ttttgcaaaa gaaagtccgt gatccacgtg tccaaggtgt gaccatcaca gatgttcaga
     9481 tgctgggtga cttgtctgtt gccaaggttt attacaccat tttgagtaac cttgcttcgg
     9541 ataaccaaaa agcccaaatc gggcttgaaa aagcaactgg taccatcaaa cgtgaacttg
     9601 gtcgcaattt gaaattgtac aaaatcccag atttgacctt cgtcaaagac gagtccatcg
     9661 agtatggaaa caagattgac gagatgctac gcaatctgga taagaactaa agaagagggg
     9721 ttgcccctct tttttggtgg aggaaaatag gttgaatttg aaatggaaaa atattctt
//
LOCUS       DAVMEF010000065         9554 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMEF010000065 DAVMEF010000000
VERSION     DAVMEF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.065"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            54..452
                     /locus_tag="VYQ30_001176"
     CDS             54..452
                     /locus_tag="VYQ30_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966286.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            920..1873
                     /locus_tag="VYQ30_001177"
                     /pseudo
     CDS             920..1873
                     /locus_tag="VYQ30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1948..2148
                     /locus_tag="VYQ30_001178"
     CDS             1948..2148
                     /locus_tag="VYQ30_001178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1966287.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            2183..2899
                     /gene="atpB"
                     /locus_tag="VYQ30_001179"
     CDS             2183..2899
                     /gene="atpB"
                     /locus_tag="VYQ30_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1966288.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            2913..3407
                     /gene="atpF"
                     /locus_tag="VYQ30_001180"
     CDS             2913..3407
                     /gene="atpF"
                     /locus_tag="VYQ30_001180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1966289.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            3407..3943
                     /locus_tag="VYQ30_001181"
     CDS             3407..3943
                     /locus_tag="VYQ30_001181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1966290.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            3958..5463
                     /locus_tag="VYQ30_001182"
     CDS             3958..5463
                     /locus_tag="VYQ30_001182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1966291.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            5479..6357
                     /locus_tag="VYQ30_001183"
     CDS             5479..6357
                     /locus_tag="VYQ30_001183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1966292.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            6443..7849
                     /gene="atpD"
                     /locus_tag="VYQ30_001184"
     CDS             6443..7849
                     /gene="atpD"
                     /locus_tag="VYQ30_001184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1966293.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            7860..8279
                     /locus_tag="VYQ30_001185"
     CDS             7860..8279
                     /locus_tag="VYQ30_001185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1966294.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(8324..9337)
                     /locus_tag="VYQ30_001186"
     CDS             complement(8324..9337)
                     /locus_tag="VYQ30_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1966295.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         2947 a         1748 c         2082 g         2777 t
ORIGIN      
        1 taaattcctt aatcgatttg cgcatgtttt atttcatttc attatacttt agattggaaa
       61 ataaggaatc acataaatcg caagaaatag ggaatctgat tcgtgtttat aaccgttcaa
      121 aaagggaaga ggctgaaagt gagccactta atctttatgt cgaagatgaa aagggcaatc
      181 tccttgcggg tttgatagca gagacttttg gaaattggct agaaatcgag tatttgtttg
      241 taaaagagga actgcgaggg caaggaatcg gttcaaaact attgcaacag gcagaaagtg
      301 aagctaagaa tcgaaattgt tgttttgcct ttgtcaatac ttatcagttc caggcaccgg
      361 acttttatca gaaacatggc tacaaggaag ttttttcttt gcaagactat ctctacatta
      421 ggcaaagata ttattaccaa aagaatctgt aaggtgagta tggaggtata atacaaagag
      481 aggctcgcac ataagtatgg aggtggactg atgtatatga gtaactatcg ataaatacca
      541 gttataacct agaaattgtg tctttctaga cgatattgag gacaatataa tcgtagctcc
      601 aaaattagga atcaaggctt atcaggttaa gaaaagaagt gatgttgttg atattttaaa
      661 acaatttatt taaactaaaa actctctatc tttttaaatt gacattaatc cgagattccc
      721 ctctcggatt ttgtgttgct ttcattaaat ttacaggtaa gacttgctag cttcagtaaa
      781 tatacacaag tttttcagaa tagagtgaaa tccgaaagaa aatggaaata atgaaagctc
      841 ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa acacaagaaa
      901 ggcacagaga tgcaaaacca ttatactaca aaaggcgaac aaagggaaat caaatagaga
      961 aattgcctct ctgcttggaa aagctcctca aactatccac actaaaatca agcgtaggac
     1021 agtccgaaaa tgtcttggaa aagggcgctt caaagaggtt tattctgccg actatgctca
     1081 acagtcttat gaaaataatc gcaagcactc ggtcaagaga tcaagcgtga ccaaggaact
     1141 aaaggaaaag attctccact atcataacca aaaatttttg cctgaaatga tggttatggc
     1201 taaaggggtt aacgtgggaa tttcaaccat ttactattgg attcatcatg gaaaattggg
     1261 gtaaagcaag caggatctgc tttaccctag aaaaggaaaa gcgcttaaga aacaggccag
     1321 caccaacttt aaacctgctg gtcaatccat cgaacagcgg cctgaagcta tcaatcttcg
     1381 cttggagaat gggcattatg agattgatac ggttctactt acgagagcga aaaactactg
     1441 cttgcttgtc ttgacggatc gaaaaagtag acatcagatc atccgattga ttccaaataa
     1501 aagtgctgag gtggtcaatc aggctctaaa actcatctta aaacaacaca agattctttc
     1561 catcacggca gataatggaa cggaattcaa tcgcttgtct aatgtatttt ctgaggagca
     1621 catttattat gcacatccct atgcctcttg ggaaagggga actaatgaga atcacaacag
     1681 gctcattcgt agatggttac ctaagggaac caagaaaatg actcccaaag aagtcgcatt
     1741 catcgaaaag tggattaaca actatcctaa aaaatgcttg gactacaagt cacccagaga
     1801 agacttctgg atggctaact tgaacttgaa atttagcaaa atggaaataa tttttattaa
     1861 acgcttccaa tagcggttaa aagttgacaa atttaaattt taggactaca ctaagtaagt
     1921 aaattttatt ttaaaggaga atttgttatg aatttaacat ttttaggctt atgtattgcc
     1981 tgtatgggcg tatctgtcgg tgaaggttta ttgatgaatg gactgtttaa atcagtagca
     2041 cgccaaccag atatgctttc tgagtttcgt agtttgatgt ttttaggtgt tgcctttatt
     2101 gaaggaactt tctttgtaac tcttgtcttc tcatttatta tcaaataaat acatggaacg
     2161 agaagaaaag ggaggatttt agatggaaga aagtattaat ccaatcatct ctattggtcc
     2221 tgttatcttc aatctgacta tgttagccat gactttgttg attgtgggag ttatttttgt
     2281 ctttatttat tgggcaagcc gcaatatgac cttgaaaccc aaaggaaagc aaaatgtact
     2341 tgagtatgtc tatgactttg ttattggatt tacagaacct aacattggtt cgcgctacat
     2401 gaaagattac tcactctttt tcctttgttt attccttttc atggtgattg ccaataacct
     2461 tggcttaatg acaaagattc aaacgatcga tgggactaac tggtggagtt cgccaaccgc
     2521 taatttacag tatgacttaa ccttatcttt tcttgtcatt ttgttgacac atatagaaag
     2581 cgttcgtcgt cgtggattta aaaaaagtat aaaatctttt atgagtcctg tttttgtcat
     2641 accgatgaat atcttggaag aatttacaaa cttcttatct ttggctttgc ggatttttgg
     2701 gaatatcttt gcaggagagg tcatgacgag tttgttactt cttctttccc accaagctat
     2761 ttattggtat ccagtagcct ttggagctaa tttggcttgg actgcatttt ctgtctttat
     2821 ttcctgcatc caagcttatg tttttactct tttgacatct gtgtatttag ggaataagat
     2881 taatattgaa gaggaataga aaggagtaac tgatgcacgt aacagtaggt gaattaattg
     2941 gtaattttgt tttaatcact ggctctttta ttcttttgct agtcttgatt aaaaaatttg
     3001 catggtctaa tattacaggc attttcgaag aaagagctga aaaaattgct tcagatattg
     3061 acagagctga agaagcccgt caaaaagcag aagtattggc tcaaaaacgc gaagatgaat
     3121 tggctggtag ccgtaaagaa gctaagacaa tcattgaaaa tgcaaaggaa acagctgagc
     3181 aaagtaaggc taatatctta gcagatgcta aactagaagc aggacactta aaagaaaaag
     3241 ccaatcaaga aattgctcaa aataaagtag aagctttaca gagtgttaag ggtgaggtcg
     3301 cagatttgac catcagctta gctggtaaaa tcatctcaca aaatcttgac agtcatgccc
     3361 ataaagcact cattgatcag tatatcgatc agctaggaga agcttaatgg acaagaaaac
     3421 agtaaaggta attgaaaaat acagcatgcc ttttgtccaa ttggtacttg aaaaaggaga
     3481 agaagaccgt atcttttcag acttgactca aatcaagcaa gttgttgaaa aaacaggtct
     3541 gccttctttt ttaaaacaag tggcagtaga cgagtcggat aaggaaaaaa caattgcttt
     3601 tttccaagat tctgtgtcgc ctttattaca aaactttatc caggttctgg cctacaatca
     3661 cagagcaaat cttttttatg atgtgcttgt agattgcttg aaccgacttg aaaaagaaac
     3721 aaatcgattt gaagtgacga ttacgtctgc tcatcctcta actgatgaac agaagactcg
     3781 tttgctccct ttgattgaga aaaaaatgtc tctgaaagta aggagtgtaa aagaacaaat
     3841 cgatgaaagt ctcattggtg gttttgtcat ttttgccaat cacaagacaa ttgatgtgag
     3901 tattaaacaa caacttaaag ttgttaaaga aaatttgaaa tagaaagtgg tgttcttttg
     3961 gcaattaacg cacaagaaat cagcgcttta attaagcaac aaattgaaaa tttcaaaccc
     4021 aattttgatg tgactgaaac aggtgttgta acctatatcg gggacggtat cgcgcgtgct
     4081 catggccttg aaaatgtcat gagtggagag ttgttgaatt ttgaaaacgg ctcttatggt
     4141 atggctcaaa acttggagtc aacagacgtt ggtattatca tcctaggtga ctttacagat
     4201 atccgtgaag gcgatacaat ccgccgtaca gggaaaatca tggaagtccc tgtaggtgaa
     4261 agtctgattg gtcgtgttgt ggatccgctt ggtcgtccag ttgacggtct tggagaaatc
     4321 cacactgata aaactcgtcc agtagaagca ccagctcctg gtgttatgca acgtaagtct
     4381 gtttcagaac cattgcaaac tggtttgaaa gctattgacg cccttgtacc gattggtcgt
     4441 ggtcaacgtg agttgattat cggtgaccgt cagacaggga aaacaactat tgcgattgat
     4501 acaatcttga accaaaaaga tcaagatatg atctgtatct acgtcgcgat tggacaaaaa
     4561 gaatcaacag ttcgtacgca agtagaaaca cttcgtcagt acggtgcctt ggactacaca
     4621 atcgttgtga cagcctctgc ttcacaacca tctccattgc tcttcctagc tccttatact
     4681 ggggttgcta tggcggaaga atttatgtat caaggtaagc atgttttgat tgtatacgat
     4741 gatctatcaa aacaagcggt agcttatcgt gaactatcgc tcttgcttcg tcgtcctcca
     4801 ggtcgtgaag ccttcccagg ggatgttttc tatctccaca gccgtttgct tgagcgctca
     4861 gctaaagttt ctgatgaact tggtggtgga tcaattacag ccctaccatt tatcgagaca
     4921 caagcaggag atatctcagc ctatatcgca accaacgtga tttctatcac tgatggacaa
     4981 atcttccttg gcgatggcct cttcaatgca ggtattcgtc cagccatcga tgcgggttca
     5041 tctgtatctc gtgtaggtgg ttctgcacaa atcaaagcca tgaagaaggt tgctggtaca
     5101 cttcgtatcg accttgcttc ataccgtgag ttggaagcct ttactaagtt tggttctgac
     5161 ttggacgcag caacacaggc taagttgaac cgtggacgtc gtaccgttga ggtcttgaaa
     5221 caacctgttc acaaaccatt acctgttgag aaacaagtaa ccattcttta tgctttgaca
     5281 catggtttct tggatactgt tccagtagat gatattgttc gtttcgagga agagttccat
     5341 accttctttg atgctcaaca tccagagatt ttggaaacca ttcgtgatac aaaagacttg
     5401 ccagaagaag cagtcttgga tgctgcgatt acagagtttc tcaatcaatc tagcttccaa
     5461 taagaataga ggtgtcagat ggcagtgtct ctaaatgata ttaaaacaaa aatcgcctca
     5521 acaaaaaata cgagtcaaat cactaatgcc atgcaaatgg tatcggctgc taagttaggt
     5581 cgctctgaag aagctgctcg caacttccaa gtttacgctc agaaagtgcg taaacttttg
     5641 acagatatcc ttcatggtaa tggagctggt gcttcaacta atccgatgtt gattagccgt
     5701 tctgtgaaga agacaggcta tatcgttatc acttcagacc gcggtttggt tggaggttat
     5761 aattcctcta ttttgaaagc tgttatggag ttgaaagaag aataccaccc agacggtaaa
     5821 ggttttgaaa tgatctgtat cggtgggatg ggagctgatt tctttaaggc tcgcggtatt
     5881 caaccacttt atgaattacg tggcttggca gaccaaccta gctttgatca agttcgtaag
     5941 attatttcaa aaactgttga aatgtaccaa aatgaactct ttgatgagct ttatgtttgc
     6001 tacaaccacc atgtcaatac gctaaccagt caaatgcgtg tggaacaaat gcttccgatt
     6061 gttgacttgg atccaaatga agcggatgaa gagtacagct tgacttttga attggaaacc
     6121 agccgagaag aaattctgga gcagttgttg cctcagtttg cagaaagtat gatttacggt
     6181 gccattatcg atgccaagac agctgagaat gctgcgggta tgacagccat gcaaacagcg
     6241 acagataatg ctaagaaagt catcaatgat ttgacaattc agtataaccg tgccagacag
     6301 gcggcgatta cacaagaaat tacagaaatc gtagcaggtg ctagtgcctt agaataggct
     6361 ctagtccagc tcgtatgaaa atgaacttag gacctagttg agctaggaac cgacagtatc
     6421 ttatatagaa taggagaagg agatgagttc aggtaaaatt gctcaggtta tcggtcccgt
     6481 tgtagacgtt ttgtttgcag caggggaaaa acttcctgag attaacaatg cacttgtcgt
     6541 ctacaaaaat gacgaaagaa aaacaaaaat cgtccttgaa gtagccttgg agttaggaga
     6601 tggtatggtt cgtactatcg ccatggaatc aacagatggg ttgactcgtg gaatggaagt
     6661 attggacaca ggtcgtccaa tctctgtacc agtaggtaaa gaaactttgg gacgtgtctt
     6721 caacgttttg ggagatacca ttgatttgga agctcctttt acagaagacg cagagcgtca
     6781 gccaattcat aaaaaagctc caacttttga tgagttgtct acctcttctg aaatccttga
     6841 aacagggatc aaggttattg accttcttgc cccttacctt aaaggtggta aagttggact
     6901 tttcggtggt gccggagttg gtaaaaccgt cttaatccaa gaattgattc acaacattgc
     6961 ccaagagcac ggtggtattt cagtatttac tggtgttggg gaacgtactc gtgaggggaa
     7021 tgacctttac tgggaaatga aagaatcagg cgttatcgag aaaacagcca tggtctttgg
     7081 tcagatgaat gagccaccag gagcacgtat gcgtgttgcc cttactggtt tgacaatcgc
     7141 tgaatacttc cgtgatgtgg aaggccaaga cgtgcttctc tttatcgata atatcttccg
     7201 tttcactcag gctggttcag aagtatctgc ccttttgggt cgtatgccat cagccgttgg
     7261 ttaccaacca acacttgcta cggaaatggg tcaattgcaa gaacgtatca catcaaccaa
     7321 gaagggttct gtaacctcta tccaggctat ctatgtgcca gcggatgact atactgaccc
     7381 agcgccagca acagccttcg ctcacttgga ttcaacaaca aacttggaac gtaagttggt
     7441 acaattgggt atctacccag ccgttgaccc acttgcttca agctcacgtg ccttggcacc
     7501 tgaaatcgtt ggagaagagc actatgcagt tgctgctgaa gtaaaacgtg tccttcaacg
     7561 ttaccatgaa ttgcaagata tcattgctat ccttggtatg gatgagcttt ctgatgaaga
     7621 aaagaccttg gttgctcgcg cccgtcgtat ccagttcttc ttgtcacaaa acttcaacgt
     7681 tgcggaacaa tttactggtc agccaggttc ttatgttcca gttgctgaaa ctgtacgtgg
     7741 ctttaaggaa atccttgatg gtaaatacga ccacttgcca gaagatgcct tccgtggtgt
     7801 aggttctatc gaagatgtga ttgcaaaagc tgaaaaaatg ggattttaag aggtgatcta
     7861 tggctcagtt aactgtccag atcgtgacac cagatggtct cgtctatgat caccatgcca
     7921 gctatgtatc ggttcgaact ctggatggtg agatggggat cttgccacga catgaaaata
     7981 tgattgcggt tttagcagtt gatgaagtaa aggtaaaacg tatcgatgat aaagatcacg
     8041 tgaactggat tgcagtaaac ggtggcgtta ttgaaattgc caatgatatg atcacaatcg
     8101 tcgctgactc tgcagaacgt gctcgtgata tcgatatcag tcgtgcagaa cgtgccaaac
     8161 ttcgtgcaga acgtgcaatt gaagaagcac aagacaaaca tttgattgac caagaacgtc
     8221 gtgctaagat tgctttgcaa cgtgctatta accgtattaa tgtcggaaat agactataag
     8281 aaaaaatgaa cttgaaaata ccaagttcat tttttatggt gttttaagga gcaaaacgga
     8341 tgcagactgc ttcgggaaca tggaagtcgt tggagagttc tgctagacga ccattgtcac
     8401 aattacgttt aaagacagtt gcattgtcag agtcttgatg gacaacaatg agaaattttt
     8461 ggtcgggtgt caaatcaaaa tcacgtggag tctgaccatg cgttggaacg atttctaata
     8521 actctaagct accgtccgca aggatggtat atactgcgat agaatcatgg ccacggttag
     8581 aagcgtagag gtatttaccg tctttagaga gatgaatagc agcggttcca ttaaagcctt
     8641 cgtaagcttc tggtaaagtt gaaataacct gcatacgttc aaattcgcca acgccatcgt
     8701 agattaaaac ttcgatagta ctattgagtt cacaaatgag ataagcgatt ttatagtggt
     8761 tatggaaaat gatatggcgt gagcctgctc ctggcttgct gtgataggta tagagcttag
     8821 ataattttcc ttcttgatcg aggtcatagg tgatgacttg gtcagttccc aagtcgcagg
     8881 tcactagata gtggtcaggt gttaaatctg tatagtgaac atggggggaa gcttgatttt
     8941 catgtggacc ttggccactg tgttgatcca tatcactaag tagaagacta ccatcttcct
     9001 ggcgtttata aacaaggact tgtcccttgt gatagttggc tgcgtaaacc aaatcacgct
     9061 tttcatcgac agcaacataa cagtggggag ctccttcttc aacaacatga tttaacacag
     9121 tcccgtcagt ttgataggct gcaattcccc ccttatcgtc ttggctacca acagtgtata
     9181 aatgttggtg ctggtcaaag gcaaggtagg ttggacttgg ctcagctgca aaaagttcta
     9241 gatttgaaag ctgaccagtt tctgtatcaa agtctgcctt gtaaatccct tgagaagtac
     9301 gacgtgtata agttccaaaa taaacagttt ctttcattac tatacctctg tgtaaagata
     9361 agactattat atcacaaaaa caagcaaatt aaagacattc aattagatgt aagcacttta
     9421 aaaaagagtt attttgtttc aaaaatggta taatgagaga acaatagaaa ggaagtattt
     9481 atggagcaaa aagagaaaca ttttagccta tcttggtttt tcaagtggtt tttagataac
     9541 aaggcaatta cggt
//
LOCUS       DAVMEF010000066         9479 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMEF010000066 DAVMEF010000000
VERSION     DAVMEF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.066"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            42..1715
                     /locus_tag="VYQ30_001187"
     CDS             42..1715
                     /locus_tag="VYQ30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1966296.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLATRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1827..2918
                     /gene="ulaG"
                     /locus_tag="VYQ30_001188"
     CDS             1827..2918
                     /gene="ulaG"
                     /locus_tag="VYQ30_001188"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1966297.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3032..5008
                     /gene="tkt"
                     /locus_tag="VYQ30_001189"
     CDS             3032..5008
                     /gene="tkt"
                     /locus_tag="VYQ30_001189"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1966298.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            5125..5424
                     /gene="yajC"
                     /locus_tag="VYQ30_001190"
     CDS             5125..5424
                     /gene="yajC"
                     /locus_tag="VYQ30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1966299.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5471..5899
                     /locus_tag="VYQ30_001191"
     CDS             5471..5899
                     /locus_tag="VYQ30_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1966300.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5901..6311
                     /locus_tag="VYQ30_001192"
     CDS             5901..6311
                     /locus_tag="VYQ30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966301.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6611..9262
                     /gene="adhE"
                     /locus_tag="VYQ30_001193"
                     /gene_synonym="adhC"
     CDS             6611..9262
                     /gene="adhE"
                     /locus_tag="VYQ30_001193"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1966302.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFRPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3025 a         1751 c         1984 g         2719 t
ORIGIN      
        1 agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg gataaggcaa
       61 gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt atggcgattt
      121 cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa atcaatgctg
      181 ctctgggtga cgaggcgctt cacatcatta gtattccacg aattggtatt cacttaacgg
      241 aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac gattatatca
      301 tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct aaagaacgta
      361 ttacgattga aaaattgata gagttaacag aggtatctag gaatactgtt ctcaatgatt
      421 tgaatagtat tcgttatcaa ctaactttgg aacaatatca ggtgaccttg caagtgagca
      481 agtcacaggg ataccacctt catgcccacc ctcttaataa aattcagtat cttcaatcgc
      541 ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta gaagataaga
      601 tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa ttttttaagg
      661 aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat gaaataactt
      721 ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa cagtatcaag
      781 aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata gagtatcagg
      841 tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg tcaggacttg
      901 aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat attcatgcag
      961 aaagtgatga ttttcggcaa ttaaaacttg ctttagaaga atttatctgg tattttgaat
     1021 cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg atgatccact
     1081 gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct ctaacaaaac
     1141 aaattcgatc caagtatgga gaattatttt tagccactag aaaatctgcg gaaattttag
     1201 aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg attcatattg
     1261 gaggattttt aaaatataca ccatcgtctc aaaaaaatat gaaaaaagtt tatctcgttt
     1321 gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt tattttccaa
     1381 atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa gatattgcac
     1441 aagttgatgt agtgattact actaatgatg atttggatag cagatttccg attttaaggg
     1501 ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt aaacacaata
     1561 tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt tcgtcttata
     1621 ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt ataaatcaag
     1681 aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga tgaacacaaa
     1741 cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat ctcaaagata
     1801 acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca agagagtcat
     1861 ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc gaagaagaag
     1921 tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact tggattaaga
     1981 caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa tcaaccaaaa
     2041 aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt cgtaagctgc
     2101 aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac gaactagact
     2161 attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct gcagcaattc
     2221 tcaataatcc taagttagag catgttaagt ttatcggtcc ttaccactgt ggacgaatct
     2281 gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt gacactatcg
     2341 aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc ttggtaactc
     2401 tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct gttacagatg
     2461 aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga accatctatc
     2521 atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac tttaaaattg
     2581 atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa atgacatcta
     2641 tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca gttcactatg
     2701 atatctggtc taacttcatg gcttctacta atgagattct agaactttgg aaaatgcgaa
     2761 aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt aagtacactt
     2821 atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat gattgttttg
     2881 aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt ctttggaggt
     2941 tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga atgtgtgatt
     3001 caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta atgcaattcg
     3061 ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg tggttatggg
     3121 agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc cagctcaacc
     3181 aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa tgctccttta
     3241 tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta agagtttccg
     3301 tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag ggattgatgc
     3361 tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc aagcagaacg
     3421 tttcttggca gccaaatata accgtgaagg ttacaatatc tttgaccact atacttacgt
     3481 tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt catacgcagg
     3541 cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca acttggatgg
     3601 tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct acggttggca
     3661 tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta tcgaaacagc
     3721 aaaagcttca ggcaagccat ctttaattga agtgaagacg gttattggat atggttctcc
     3781 aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg aaactgcatc
     3841 aactcgtcaa gccctcggtt gggactacga accatttgaa attccagaac aagtatatgc
     3901 tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt ggactaaatt
     3961 agttgcagat tataaagaag ctcatccaga actggctgca gaagtagaag ccatcatcga
     4021 cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa atggtttttc
     4081 tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta aattaccaac
     4141 cttcctaggt ggatcagctg accttgctca ctcaaacatg acttatatca agacggatgg
     4201 acttcaagac gatgccaatc gcttgaatcg taacattcag tttggtgttc gtgaatttgc
     4261 aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat acggtggaac
     4321 tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct tacaaggact
     4381 tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg gtccgactca
     4441 cgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg ttttccgtcc
     4501 agcagatgcg cgtgaaactc aagcagcttg gtaccttgca gtgacaagtg agaaaacacc
     4561 aactgccctt gtcttgacac gtcaaaactt gactgttgaa gatggaacag acttcgacaa
     4621 ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata ccatcttgat
     4681 tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg ctagtcaagg
     4741 cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac aagatgcagc
     4801 ttacaaggaa gagattcttc caaatgcagt ccgccgtcgt gttgcagtcg aaatgggtgc
     4861 aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta ttgatacctt
     4921 cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg tagaaaatct
     4981 tgtaaaaatt gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc tctggttttt
     5041 cttaccagaa aagtaaggta caatcttgta aaagtagctg aaatttgata tagtagtcct
     5101 atgtaaaaga caaaggagaa gacaatgaat ccaaatatta cttttttaat catgcttgta
     5161 ggtatgatgg ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc tcaaaagcgt
     5221 atggaaagct taaataaact acaaaaaggc tatgaagtga ttacaatcgg tggactttat
     5281 ggaacagtcg atgaagtaga tacggagaag ggaacaatag ttcttgacgt agatggagtt
     5341 tacttgactt ttgaactagc tgctatcaag acagtattac cgctgaaaga aacagcttca
     5401 ctagaaggcg caattgaaaa ataagacggg atcacgaact cccgtttttc tataaaagaa
     5461 aggaaatggg atgaaaaaat tagtctttgt ctgtctggga aatatttgcc gtagccctat
     5521 ggccgagttt gttatgaaat caatgacaga taactacgaa atccaaagtc gagcaacttc
     5581 ctcttgggaa catggcaatc cgattcataa ggggactcag ggaatttttc aagagtatga
     5641 gattccttat gacaagaaca agacatcgct tcagattagt aaggaagatt ttgaagcctt
     5701 tgattatatt atcggaatgg acgcttcaaa tatttctgac ttacgtcaga tgtgtccagt
     5761 agactgtcaa gataagattt actcattttc atctgaaagt gttccagacc cttggtatac
     5821 aggagatttt gaagaaacct atcgacgtgt tcaagagggc tgtcaagctt ggttagaacg
     5881 tttagaaaag gagagttaag gtggaaaatc ttacgaattt ttacgaaaag tatcgtgtct
     5941 atctgactcg tccacgttta gagcttttgg cagtagttac cattgttttc tgtgctgtac
     6001 tcgtcttttt tctaaatatt ccaggaaaag gtgtcttaaa actcgataat ggaacgattg
     6061 tttatgatgg cagtcttgtc cgtggtaaaa tgaatggcca aggtaccatt accttccaaa
     6121 atggagacca atatacaggt ggcttcaaca atggagcctt caacggaaaa ggtacctttc
     6181 aatctaaaga aggctggacc tacgaaggtg attttgtaaa tggtcaggct gaaggaaaag
     6241 ggaaactaac aacagaacaa gaagtcgttt atgaaggaac ttttaaacaa ggcgtttttc
     6301 aacaaaaata aagcctcctt atcaaaggag gtattattag aatcacaagg taagcgctta
     6361 cctgtaaatc cctttctttc caaatccctc ttccaagcaa gtttgtgaaa taaaaaatat
     6421 ttgaaataaa tttcacaaac ttcaaagata aaacttgata agaaaagaaa atgagaaaag
     6481 tttcacaagg gtttaaaaat tctttgtgaa atgtttatga aactgtttac aaagttgaaa
     6541 taatgcgtta taataaactt gtgaaaaaat taacaaagga tatcgttcct tgaaagctat
     6601 ggaggaaaat atggctgata aaaaaactgt gacaccagag gaaaagaaac tcgttgctga
     6661 aaaacacgta gatgagttgg ttcaaaaagc tctagttgcc cttgaagaaa tgcgtaaatt
     6721 ggatcaagaa caagttgact acatcgttgc caaagcatca gtagcagctt tggatgccca
     6781 cggagaattg gctttacatg cctttgaaga aacaggacgt ggtgtatttg aagacaaagc
     6841 aactaagaac ttgtttgctt gtgaacacgt agtaaacaat atgcgccaca ctaagacagt
     6901 tggcgttatc gaagaagacg atgtaacagg attgactctt attgctgaac cagttggtgt
     6961 tgtttgtggt attactccaa caacaaaccc aacatcaaca gcaatcttca aatcattgat
     7021 ttcattgaag acacgtaacc caatcgtctt tgccttccat ccatcagcac aagaatcatc
     7081 tgctcatgca gctcgtatcg tccgcgatgc agctatcgca gctggtgctc ctgaaaactg
     7141 tgtgcaatgg attactcaac catctatgga agcaacaagt gcccttatga accacgaagg
     7201 tgttgcgaca atccttgcaa caggtggtaa tgccatggtt aaggcggctt attcatgtgg
     7261 taaaccagct cttggggtag gtgccggaaa cgttccagct tatgttgaaa aatcagcaaa
     7321 cattcgtcaa gcagcacacg atatcgtcat gtctaaatca tttgataacg gtatggtctg
     7381 tgcatctgaa caagcagtta tcattgataa agaaatttac gatgaatttg tagcagagtt
     7441 caaatcttac cacacttact ttgtaaacaa aaaagaaaaa gctcttcttg aagagttctg
     7501 cttcggcgtc aaagcaaaca gcaaaaactg tgctggtgca aaattgaacg ctgacatcgt
     7561 tggtaaacca gcaacttgga ttgcagaaca agcaggattt acagttccag aaggaacaaa
     7621 cattcttgct gcagaatgta aagaagttgg cgaaaatgag ccattgactc gtgaaaaatt
     7681 gtcaccagtt attgcagttt tgaaatctga aagccgtgaa gatggtatta ctaaggctcg
     7741 tcaaatggtt gaatttaacg gtcttggaca ctcagcagct atccacacag ctgacgaaga
     7801 attgactaaa gaatttggta aagctgttaa agctattcgt gttatctgta actcaccttc
     7861 tacttttggt ggtatcgggg acgtttacaa tgccttcttg ccatcattga cacttggatg
     7921 tggttcttac ggacgcaact cagttgggga taacgttagt gccattaacc tcttgaatat
     7981 caaaaaagtc ggaagacgga gaaataacat gcaatggatg aaacttcctt caaaaacata
     8041 ctttgaacgt gattcaattc aataccttca aaaatgtcgt gacgttgaac gtgtcatgat
     8101 cgttactgac catgccatgg tagagcttgg tttccttgat cgtatcatcg aacaactgga
     8161 ccttcgtcgc aataaggtcg tttaccaaat ctttgcggat gtagaaccgg atccagatat
     8221 cacaactgta aaccgtggta ctgagattat gcgtgccttc agaccagata ccatcatcgc
     8281 actcggtggt gggtctccaa tggatgctgc caaagtaatg tggcttttct acgagcaacc
     8341 agaagtggac ttccgtgacc ttgtccaaaa attcatggat atccgtaaac gtgccttcaa
     8401 gttcccattg cttggtaaga agactaaatt catcgcgatt ccaactacat ctggtacagg
     8461 atctgaagta acaccatttg ccgttatctc tgataaagca aacaaccgta aatacccaat
     8521 cgctgactac tcattgacac caactgtggc aattgtagac cctgctttgg tattgacagt
     8581 tccaggattt gttgctgctg atactggtat ggacgtattg actcacgcga cagaagcata
     8641 cgtatcacaa atggctagtg actacactga tggtttagca cttcaagcta ttaaattggt
     8701 ctttgaaaat ctcgaaagct cagttaagaa tgcagacttc cactcacgtg agaaaatgca
     8761 taacgcttca acaatcgctg gtatggcctt tgccaatgcc ttcctaggta tttctcactc
     8821 aatggcccat aagattggtg cgcaattcca cacagttcac ggacgtacaa atgccatctt
     8881 gcttccgtac gttatccgtt acaacggtac acgtccagct aagacagcaa catggcctaa
     8941 gtacaactac taccgtgcag atgaaaaata ccaagatatc gcacgcatgc ttggacttcc
     9001 agcttctact ccagaagaag gggttgaatc ttacgcaaaa gctgtctacg aactcggtga
     9061 acgtattggg atccaaatga attttagaga ccaaggaatt gacgaaaaag aatggaaaga
     9121 acattctcgt aaattagcct tcctggctta tgaagaccaa tgttcaccag ctaacccacg
     9181 tcttccaatg gtagaccata tgcaagaaat catcgaagat gcatactatg gctacaaaga
     9241 aagaccagga cgccgtaaat aattgtttat cagtctagaa gcaagacaaa aactcaattt
     9301 gagggaaaga tccagtaatt tttctatgat aaaaggcatc ctatcaaggt ttttgaatac
     9361 ctgataggat gcctttttat gatgttgagg cctttttgcc ctttttgaaa aactagaata
     9421 gaaacaaaat atatagtagg ttgaaactag aatagtacac atctgctgct aaaacattt
//
LOCUS       DAVMEF010000067         9095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMEF010000067 DAVMEF010000000
VERSION     DAVMEF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.067"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(422..2701)
                     /gene="pulA"
                     /locus_tag="VYQ30_001194"
     CDS             complement(422..2701)
                     /gene="pulA"
                     /locus_tag="VYQ30_001194"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1966303.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEENLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(2813..4771)
                     /gene="ligA"
                     /locus_tag="VYQ30_001195"
     CDS             complement(2813..4771)
                     /gene="ligA"
                     /locus_tag="VYQ30_001195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1966304.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(4865..6043)
                     /locus_tag="VYQ30_001196"
     CDS             complement(4865..6043)
                     /locus_tag="VYQ30_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1966305.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(6123..6986)
                     /locus_tag="VYQ30_001197"
     CDS             complement(6123..6986)
                     /locus_tag="VYQ30_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1966306.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(6989..8890)
                     /locus_tag="VYQ30_001198"
     CDS             complement(6989..8890)
                     /locus_tag="VYQ30_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1966307.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKFSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         2744 a         2005 c         1623 g         2723 t
ORIGIN      
        1 aaataaaaaa atccactacg tcaaggcgag gctaatgtgg tttgaagaaa ttttcgaaga
       61 gcgtgaatga gtatcatcta tagtaaaata aaaaaactga acaatttggt tggggacagc
      121 caaaccaatt tctcacaatg ttttagaaac aagggtgtgc tattccaatt tcagcctact
      181 ataactgtca tagattgctg aaacaaagtc taggtaaaag tcttcataat aaaaagacct
      241 cctatcaaat attcaaaaac tttgatagga ggtcttgttt cgtgaaaata tttatcaaat
      301 tttctataca agtgagctgt tagccaggtt ctttctattc tttcaatttc aatgaatgga
      361 ttttttacca atactcataa ctgggaattt ttctgtctaa aaatagcgag atagatggta
      421 tttataaaac actcaagaca gctagactaa tatcatttaa aacattatct tcttttgagc
      481 gactgttggt taccaacata gctaaatttc ctgcattttc aaattgatag ggttctgatt
      541 tagcattcac aactaccaag aggtgttctt tgccgtgaac ttcatagata aggcagccgc
      601 tatgttcaat cgcagaatgc acaaagacat gatggtaaat ttcatcatag ctagagtaag
      661 aaaaggcacc agtttttgtc ttcaatcgga tgacttgacg gataaactca atactgtctt
      721 gacgctcatt aatcaagttc cagttcactt ggttcacact gtcaggagca ttatagctat
      781 tcatcgcacg ctctctatca tcatgggtca actcaccatt ttcaccagtc gcaaccagtt
      841 tggtacgacc aaattcttga ccgatttcca taaaggccat cccctgcatg agcagattca
      901 tggctgtggc agtttcgacc ttgcgcatga tttgctctga actttggtct ggatgaaggg
      961 ttgccaataa atcgtgaaga ttgtaattgt catgggcttc tacatagtta agcacctgat
     1021 ttggatgtgt atagcttcct aattcacgac ttcctaggat tgctttagct agaattggct
     1081 ctgtcgcagc accactgaca aaacctgact tgatagcacc ataaacttct ccccctttga
     1141 cagcatcgcg ctgattgtca ttaaagaaac caatatttgg catctggtag gcattgtcct
     1201 tcttggcctt atcataaggg gcaagacctg ttcccatatc ccatccttct ccatagagga
     1261 taatgttgga gtcgatttca tccaagcttt gacgaatcat ctgcatggtc ttgacatcat
     1321 gaatccccat caagtcaaaa cggaagccgt caatattata ttcctgcacc cagtatagaa
     1381 gagaatcaat catatacttg cgaaacattt cgtgttcact ggctgtttca tttccaacac
     1441 ccgttccatt ctggaaggta ccatctggat tcatacgata atagtaatca gggactgttg
     1501 tttggaatgg tgcatcaaca actgagaagg tatggttata gactacatcc ataatgactc
     1561 caatacccgc atcgtgataa gcttgaacca tcaccttcaa atcacgaatg acctgagctg
     1621 gatcatctgg attagttgaa aaactagttt ctggcgcgtt atagttttgt ggatcataac
     1681 cccagttgta ggttacattt ccatcctcat cgtattcttt atgacggtct gcaattggtt
     1741 gcaattgaac ataattgtag cccagcttct tgatgtaatc aaaagcagtt gactggccgt
     1801 attggttaac tgttccagcc tgagcagcac ccaagaaagt tcctcgaaga tgttcatcta
     1861 cacccgatgt aggtgattta gtcaaatcac gaatgtgcat ttcacagata actgccttac
     1921 atggattttc caagcgccaa gtagcctccg aaccgtgctt aacctcgaag ttttcaactt
     1981 gcttttctac atggctcaga atagctgaac gtttgccatc agggctggtc gcgattgtat
     2041 aaggatcacg tgtcagtgtt tggtgatgag ggaattggac ttgatactga taagtcttac
     2101 ctaccaaatt ttcttcaaca tccaaactcc agacaccgat tgtattgtcc ttatgattat
     2161 aagagtagct attgcctctt ttcatctcaa aagtcttcca aacgggtgca tcattagcag
     2221 ctgattcata aacgacaact tgcacttctg tcgctgtagg tgaccagaga gaaaaatgag
     2281 cctgattgtc ctctacacgg caacccaatt ctccttggta accccaatga tgatcaaaac
     2341 tagcactgtt aatggcctta tcaaaggcaa aaggattttg atttttatag aaaggactgg
     2401 caatagcagg attttcagag taataaatcc tatcatcgcc ttccaaaatc cagacctctg
     2461 ttaatagggg atagtgatta aaacggatag aatattcttt actagtttga cctgtatgaa
     2521 ccacaaaatt caagctttct ataacatgtg aacttgggtg ttcaaagcta aataaagctc
     2581 caaaataatc ttctttgtag gttagcaaat caattcgttg atcctgactt tttacaaagg
     2641 agcaagtgtc atattctcca ttcttacgat ggtaatgaat gcgcataggg tagttataca
     2701 ttttttattt ttccttttta ctttgtttct atttcactaa taaatttttg tcaatctcgt
     2761 ctcaattaac agacatagtc atattctcta aactctgttt ttaaacgatc cattacaaac
     2821 tttctagcca tgcctcatct ctgacctgga taccaagttc ttgtgctttt tgcagtttac
     2881 ttccagcgtc tgcacctacc acgacgaggt cggtcttttt agaaatacta cctgtcactt
     2941 tggcacccag actttcgagt ttacttttag cttctgagcg cttgagtcgt tccaattttc
     3001 ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc tactaccgtc tgtcctttat
     3061 agtccagatt gaccccagtt tctttcaatt ctctgagcag aatttcagag ccttctgtcg
     3121 caaaataagt ctgaagactt ttggcaatca cgccacctag actttcaata ctagccactt
     3181 cctctgaatc tgcctgagac agattttcaa ttgaatggaa atattgaagt aaaagctgac
     3241 taaccttgct tccgacatga cgaattccca aaccaaataa gagcttctcg gcagaatttt
     3301 cctttgatgc ttggatagcc tgatacagtt tagcagcgga cttttcctta accccctcta
     3361 aaaggaggaa atcctcttct tgcaaacgat aaatatccgc cacatccttg actaaattag
     3421 cagcaaaaag cttctcaaca atagatggac caaggcctgt aatattcata gcatcacgag
     3481 aagcaaagtg aatcaagcct tccatgattt gagcagggca acgcggattg atacaacgta
     3541 gggccacttc atcttcaaag tgcaacaagt cagagttaca acttggacag tttgtaggga
     3601 tatctagttt ttcttcagag acccgtttgg actctaccac acgtaaaacg gcagggatga
     3661 tgtcaccagc cttatataca atgaccgtat cgtcttttcg gatatctttt tcagcaatat
     3721 aatctacatt gtgcagggtc gcacggctaa cagtcgtacc ggcaagttgt actggtgtta
     3781 gattagcagt tggagttaca acaccggtac ggccaactgt ccagtcaact gataagagtt
     3841 gagcttcttt ttcttcggca gggaacttgt aggctactgc ccactttgga gccttaactg
     3901 taaaaccaag ttcttcttga cttgctaggt cgttgacctt gattaccact ccatcaatat
     3961 cgtaaggcag attttcccgt tcctgtccta cttcttggat aaaattccag atttcatcta
     4021 tgttttcagc caagattcgc ttaggattga ccacaaaacc tagttgttct aggtacttca
     4081 aacccttttc ttggctatca cgagttgaag ggctggcttc ttgatagaga aacgttgcaa
     4141 gattacgctt ggcaactact gctgtatcca actgacgcag agttcctgct gccgcattac
     4201 gaggattagc aaattcaggc tctccatttt cttggcgcac ttggttaact tggtcaaagg
     4261 aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc tagttcttct ggcaaagtca
     4321 aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt ttcaccaatt gaaccatctc
     4381 cacgtgttac cccagcaacc aaaatcccct tttcataagt cagcgagata gataagccat
     4441 cgattttcag ctcacaaata taggtcggat gagccacttc cttacgaaca cgcgcatcaa
     4501 aagcatctag ctcctcacgt gaaaaagcat cctgcaaact ataaagagga tactgatgac
     4561 tgtatttttc aaaaccatct aaaaccttgc caccaacacg atgagtcgga ctgtctgcta
     4621 gcacttgctc tggataagca gtttctaact cgaccaactc acggtaaagg cggtcatact
     4681 cactgtctga aaccgaggga ttatcgctgg tatagtactc agtcgcatag cgattgagca
     4741 aagcgactaa ctcattcatt cttttattca taagaccatt ttaccataaa acaagccctc
     4801 ctcacaaacg agaagggcgg aaaaaaacac ttagtttgaa attatttttg aaactcaagc
     4861 aaccttatat caatttttca aaatgagttc gaacataaat aaacgatata caagacaaga
     4921 tgataacacc acttccaatt atcaggaaag aagagagatg tacacttggc aagactgtca
     4981 taaatccttt tgcaataggc ataaatagaa tagctaaggt aaaaattgta ctcagtactc
     5041 ttccaagaaa ttcgctctca accttggttt gtacttgagt aaaaaagtga atattaaaaa
     5101 tcgtcataaa caattcacaa actaaatttc cagaaaagga aagaaaagtt ggaagtggta
     5161 atcccatcat aaaaactccg acacctgtca aagccagtaa aatcaaaaga ttataaatat
     5221 tagctttaat tttactagct agaagagccc caatgatgga accaatagcc cccatagtta
     5281 aaatacttgc ataggctcct tctgacccgt aaagctgatt cgaaaaggga agtagaaatt
     5341 caaaagctgc aaaaaagaaa ttaacgctgg aagctaccag caaaaggaag aaaatttctt
     5401 gctgatgcca gatatagtgt aacccatcct tgatatctac aaaaatatct ctcccagtaa
     5461 aagccttttt ctcttgaact tttgcttcct cttttggaag gaaagccact agaacaaaag
     5521 caatgaaaaa agtcagcgag tctagcagta gcgtcatatg gagacttgca aactgtaaaa
     5581 caaggaagga aagaacagga gagctaacac ctacaacctg caaaaccagc tctaggcgag
     5641 aattatagat cacaatctca tctttctcca ccacttcagt tatgatagct ttattggctg
     5701 tgcgagaaaa ggcaaaagca atagcctgca caatgttagc aacaatcaaa gcgccaatca
     5761 tccagctatc attccttatg aaagaaatag ccagacaaag aatcccacaa acaagatctg
     5821 ccgtcattaa aatcttacga cgagaaaaac ggtctgaaat aactccgcca aagggattga
     5881 cgagaataga tgtgacgagc tcagaaatct gatacattcc taaaactgtc tgtcctatag
     5941 tccccataga agccaaccag acactatttc cataatcata gagcatattt cccattttat
     6001 tgatagcccc acggctaatc aactgcactg catagcgatt catattaaag ctcctctcaa
     6061 attttgaaac tattgtatca aaaccgaaag gagcttttta ttttttccct tatttgggaa
     6121 aattaacttt tgacaaattt ttcgtagtgt tcctgataat aggctacttg ctctggaaga
     6181 cctaacacat caaaaatatg catggcctct tgcatctgct tacagccttc tttacactgt
     6241 cctttttgat ataaggcaaa accttttaaa taatggaaaa cattacgctc ataaagctta
     6301 atacctttgt caataatctt ctctgtataa gcctcaaaat agttggcatt ataaaaagaa
     6361 gaatgctcta aacaatgctg gtaacaattg agggccaaaa tcaacactaa tctcttatgg
     6421 cgactaatct cttggtaaaa ttcctccctc tccataactt ctctaccaat ccgagtgaca
     6481 tagtctacat cgtagaaact atagaggtta ccgaaaagaa tcaactcata catggtccat
     6541 tcttctgttt tgaagagata atctgctacc ttatccaaat catcctgctt catatcataa
     6601 ctcgcatctc tttgacaaat cagaccctgt agcaaaatcc agttcagctc aaaataaagg
     6661 ggagtcgtcg aactcttaga cttttcaagt tgttctcttt gaagcttttg aaaacctgca
     6721 atatcgtttg aatagtaaag tgggataatc tgtgccatca tagacacatg ttcatgatta
     6781 tgaaaattcc ttgccttatc catgaaattt tcgattgtta catgaatgtt atccaaaatc
     6841 tcaaagaaac gggagactgc caggtcagac tccccaagct caaagcgaga taactgagag
     6901 gtagagcagg attcgcctgc tgcttccttt aaagaataat ttccacttgt tcgaaattca
     6961 cgaaatactt ttccaagatg ttccatcttt acacctgctc tgataattct tcccactcaa
     7021 gcatagcttc ttcctgacga tggctgattt tgtccagctc agcctgtaat tccatgagtt
     7081 tgtcggcatc gtttgtttcc aacatttgtt cagaaatggc ttggctttga ctttctagct
     7141 cttcaatttc agcttctaga ctttcgattt gtcgcatgag tttgcgaact tctttttgac
     7201 tttctttctg ggcctgatag tcattgactg gacttgcttc ctttgcttga ttgctagttg
     7261 aagcttcctc agtctgactc atttctgctg ttgctttctt ctcaacatag tagtcgtaat
     7321 ctccaaggta gagagttgaa ccattctcag acaattccaa aacatgagtt gccacacgat
     7381 tgataaagta acgatcatga ctgacaaaaa gcaaggtacc atcaaagtca atcaaggcat
     7441 tttctagcac ttccttacta tcaatatcca agtgattggt cggctcatcc agaatcaaaa
     7501 agttattgtt ttccatagaa aatttagcta aaagcaaacg agctttttcg ccaccagata
     7561 gcatgccgac tgatttttta acatcatctc ctgagaaaag gaaggctcca agacggttgc
     7621 ggatttcaac ttctggtgtc agtttgaaat cattccagag ttcatccagc accgtattac
     7681 ttggtgtcag cttgctttgg gtttggtcat agtaaccaac ctcaacatta gcgccaaagc
     7741 gcttttctcc cttgataaaa ggaatctggt ccacaataga cttgataaag gttgacttgc
     7801 cgataccatt tggaccaacg atagcgacag cattcatctt acgaagatct aggttgatag
     7861 gttgtgacaa gacttccccg tcatagccaa cagctgcatt ttcaacagtc aaaacaacat
     7921 tgcccgacgt tttttcagac tggaaggtca tgttggctgc tttcttgcca gcttcaggct
     7981 tgtccaaacg ctccattttt tctagttgtt tacggcgaga ttgagcacgt ttagtcgttg
     8041 aagcacgaac tagattgcga ttgacaaagt cttccagagc agcgatttcc ttctgttgct
     8101 tttcatagtt ttttgcctca gtagctagct tttgctcctt caactcaaca aaacgagagt
     8161 aattccccac atagcgatcc aaggaatgct tggtcaaatc tagcgtaatt gtcgcaacct
     8221 tgtccaagaa ataacggtcg tggctgacga taatgagggc accgctatag tttaccaagt
     8281 aattctctag ccaggagatg gtttcaatat ccaagtggtt agttggctcg tccaagacca
     8341 agagattggg cttttcaagg agcattttag ctagagccaa acgagtattt tgaccaccag
     8401 aaagctcagc aattttcatc tgccacatag actcgtcaaa cttgaatcca ttcaaaatcg
     8461 ctcgaatatc agcttcatag gtaaagccac ctgcttggcg aaaattttca gataagcggt
     8521 cataatctga catcagttta tccaaatcct caccagactt ttcacccatc tccagctcca
     8581 tctgacgcag ttgtttatct gtccgacgta agtcatcaaa gacatgaagc atctcatcat
     8641 agatggtatt ttcagactca aaacggctat cttgagctag gtaagacaga gaaatatctt
     8701 ttttcttatt gatttctcca ctagttggct cctcttctcc aactaaaatc ttcaaaagag
     8761 tagacttacc tgcaccattt ttcccaacaa gggcaatccg atctcgttca tcaacctgca
     8821 ggttgatatt atcgaaaaga acctctcctg caaaagaacg ttcaatttta ttagcttgta
     8881 aaataatcat acaagtagta tagcatgttt ccctaaggca ttcaagataa tcgtaagtct
     8941 tttagtacaa cttttataac ataaaataaa ctaaattatg tatattttat attagattcc
     9001 ttcactatct tgttggattt tctaaccagc taatcttgtt tcaaatagtt atcgcacaag
     9061 cctattattt aattcttttc attatttacg tacgc
//
LOCUS       DAVMEF010000068         9092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMEF010000068 DAVMEF010000000
VERSION     DAVMEF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.068"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            53..736
                     /gene="rpiA"
                     /locus_tag="VYQ30_001199"
     CDS             53..736
                     /gene="rpiA"
                     /locus_tag="VYQ30_001199"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1966308.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            750..1961
                     /locus_tag="VYQ30_001200"
     CDS             750..1961
                     /locus_tag="VYQ30_001200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1966309.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            1963..2520
                     /locus_tag="VYQ30_001201"
                     /pseudo
     CDS             1963..2520
                     /locus_tag="VYQ30_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            2520..3329
                     /locus_tag="VYQ30_001202"
     CDS             2520..3329
                     /locus_tag="VYQ30_001202"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1966310.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3805..4200
                     /locus_tag="VYQ30_001203"
     CDS             3805..4200
                     /locus_tag="VYQ30_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1966311.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEIIEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4210..4785
                     /locus_tag="VYQ30_001204"
     CDS             4210..4785
                     /locus_tag="VYQ30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1966312.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            4810..5322
                     /locus_tag="VYQ30_001205"
     CDS             4810..5322
                     /locus_tag="VYQ30_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1966313.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            5980..6690
                     /gene="deoD"
                     /locus_tag="VYQ30_001206"
     CDS             5980..6690
                     /gene="deoD"
                     /locus_tag="VYQ30_001206"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1966314.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(6713..7237)
                     /locus_tag="VYQ30_001207"
     CDS             complement(6713..7237)
                     /locus_tag="VYQ30_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1966315.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(7290..7526)
                     /locus_tag="VYQ30_001208"
     CDS             complement(7290..7526)
                     /locus_tag="VYQ30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1966316.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(7594..8514)
                     /locus_tag="VYQ30_001209"
     CDS             complement(7594..8514)
                     /locus_tag="VYQ30_001209"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1966317.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            8704..>9092
                     /locus_tag="VYQ30_001210"
     CDS             8704..>9092
                     /locus_tag="VYQ30_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966318.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKY"
BASE COUNT         2739 a         1611 c         2014 g         2728 t
ORIGIN      
        1 taaaaagcgg tttcatagta aaataagaac tgagaatgat ggaggtaaat cggtggaaaa
       61 tctgaagaaa atggcaggta tcaaggctgc tgagttcgtg agcgatggaa tggtcgttgg
      121 acttggaaca ggctcgactg cctattattt tgtcgaagaa atcggtcgtc gtatcaagga
      181 agaaggattg cagattacag ctgtaacaac ttctagtgtg accactaaac aggcagaagg
      241 ccttaatatc ccgctcaagt ctattgacca agtagacttt gtcgatgtga cggttgatgg
      301 cgcggatgaa gtggatagtc agtttaatgg aatcaaaggc ggtggtggtg cccttctcat
      361 ggaaaaggtg gtcgcaacac catcaaaaga atacatttgg gtggtggatg aaagcaagct
      421 ggtcgaaaaa ctaggtgctt ttaaattgcc agtagaagtg gttcagtatg gtgcagagca
      481 ggtctttcgt cattttgaac gagctggcta caaaccaagt ttccgtgaaa aagacggcca
      541 acgttttgtg acggatatgc agaattttat cattgacctc gccttggatg tcattgaaaa
      601 tccaattgct tttggacaag aattggacca tgtcgttggt gttgtggagc atggtttatt
      661 caaccaaatg gtggataagg taatcgttgc tggacgagat ggagttcaga tttcaacttc
      721 aaaaaaagga aaatagaagg gggcataaga tgtctaaatt taatcgtatt catttggtgg
      781 tactggattc tgtaggaatc ggtgcagcac cagatgctaa taactttgtc aatgcagggg
      841 ttccagatgg agcttctgac acactgggac acatttcaaa aacagttggt ttgaatgtcc
      901 caaacatggc taaaataggt cttggaaata ttcctcgtga aactcctctt aagactgtag
      961 cagctgaaat caatccaact ggatatgcaa caaaattaga ggaagtatct cttggtaagg
     1021 atactatgac tggacactgg gaaatcatgg gactcaacat tactgagcct ttcgatactt
     1081 tctggaacgg attcccagaa gaaatcctga caaaaatcga agaattctca ggacgcaagg
     1141 ttattcgtga agccaacaaa ccttattcag gaacggctgt tatcgatgat tttggaccac
     1201 gtcagatgga aactggagag ttgattatct atacttcagc tgaccctgtt ttgcagattg
     1261 ctgcccacga agacattatt cctttggatg aattgtaccg tatctgtgaa tacgctcgtt
     1321 cgattaccct tgagcgtcct gcccttcttg gtcgcatcat tgctcgccct tatgtaggtg
     1381 aaccaggtaa cttcactcgt acggcaaacc gtcgtgactt ggctgtatct ccattttccc
     1441 caactgtttt ggataaattg aatgaggctg gtatcgatac ttatgctgtg ggtaaaatca
     1501 acgatatctt taacggtgct ggtatcaacc atgacatggg tcacaacaag tcaaatagtc
     1561 atggaattga tacactattg aagactatgg gacttgctga gtttgaaaaa ggattctcat
     1621 tcacaaacct agttgacttt gatgcccttt acggccatcg tcgtaatgct cacggttacc
     1681 gtgattgttt gcatgagttt gatgaacgct tacctgaaat tatcgcagct atgagagaga
     1741 atgaccttct cttgattact gcggaccatg gaaatgaccc aacgtatgca gggacggatc
     1801 acactcggga atatattcca ttgttggcat atagccctgc ctttaaagga aatggtctca
     1861 ttccagtagg acattttgca gatatttcag cgactgttgc cgataacttt ggtgtggaaa
     1921 ctgctatgat tggggaaagt ttcttagata aattggtata agatgacgcg ctatgctttg
     1981 ctggtgagag gtatcaatgt tggtagtaag aataaggtcg ttatggcgga gcttcgtcaa
     2041 gaattgacaa acttgggact ggaaaaggtt gagagctaca tcaacagtgg caatattttc
     2101 tttacttcga tagattccaa agcccaattg gtttaaaagc tagagacttt ctttgcagtc
     2161 cattatccat ttattcagag cttttcttta ctgagtctag aggactttga ggcggaactt
     2221 gaaaatctac cagcttggtg gagcagagac ttggcacgaa aagattttct cttttacact
     2281 gagggtttgg atgtggacca agtcatcgcg acagttgaaa gtttagagct gaaagatgaa
     2341 gtgctttatt ttagaaaact tgggattttc tgggggaaat tttctgaaga atcctattct
     2401 aagactgcct atcataagta cttgctgaag gtgcctttct accgccacat tactattcgt
     2461 aatgctaaaa cctttgacaa aattggtcaa atgctaaaaa ataataaagg agacacataa
     2521 tgacattttt aaacaaaatc catgaaactg ctactttcct gaaagaaaag ggaattgcag
     2581 cccctgagtt cggtctaatc cttgggtcag gacttggaga attggcagaa gaaatcgaaa
     2641 atccagttgt agtagactat gctgagattc caaactgggg ccgttcaaca gtagtcggtc
     2701 atgctggtaa attggtatat ggtgaactgg caggtcgcaa ggtcttggct cttcaagggc
     2761 gtttccattt ctatgaaggg aatcctctgg aagtggtgac tttcccagtt cgtgtgatga
     2821 aagttcttgg atgtgaaggt gttattgtaa ccaatgcagc tggcggtatc ggatttggtc
     2881 ctggtacctt gatggctatc tcagaccata tcaacatgac ggggcaaaat ccattgatgg
     2941 gtgaaaactt ggatgacttt ggcccacgtt tcccagatat gtctagggcc tacacaccag
     3001 aataccgtgc cactgcccat gaagtggcta aaaaacttaa tatcaagctt gatgaaggtg
     3061 tctatatcgg agttactggt ccgacttatg aaacaccagc agaaattcgt tcctataaga
     3121 cactgggagc agatgcagtt ggtatgtcta cggttcctga agttatcgtg gcggcccact
     3181 ctggcttgaa agttctggga atttcatgta tcactaactt tgcggccggt ttccaagaag
     3241 aactcaatca cgaagaagtt gtagaagtga ctgaacgtgt taaaggtgat ttcaaaggct
     3301 tgcttaaagc gattcttgct gaattgtaag aaaaaagatt taaaaggggg agtgcctctg
     3361 ttttttcagg attgactgcc tatccggatt aaagaagaaa cagaggaata ctatgagctt
     3421 cttcctgctc ttataactga aagaagcgga agaataggtg tgtctgatct gatagccagc
     3481 attgtgaaag acaagattct aggatactag cattagcttc ctagccaagt agactagtat
     3541 gataaggaga gatgagaatg aattgacttt ctgaatttct cagtcttatc atatatagca
     3601 caatgagatt tcgcttgagt ctgcttgtaa ataaacgaaa agaaagataa gaaataatga
     3661 aaattggtca acgaattatg cgctttggca taaaaaatta agtatcggag ttgtatctgt
     3721 tgtagtcggc tttgatttct agctccagct ggaatttcag ccaatgaagt aaagcaagat
     3781 gtaacatctg aagtggtaat aggtgtgcta gattctaagg aggaattgaa agagtcagaa
     3841 aatgatgctc caaaactaga aactcctctt agagaggagc caagactagc tcctcaaacg
     3901 cttccggaag caagtgaagt tcttgaaaac aaaagggaag agtcaaaagt agagataata
     3961 gagccagctc aagcggatga tatccgcaag gttgttgggg aattagccaa ggatataagt
     4021 attactaagt tgtatatgac aggtcattct cttggatgtt acctagctca gattgcagcg
     4081 gttgaagctt accaaaaata tcctgatttt tataaccatg tattgaggaa agtgacaact
     4141 ttcagtgctc ctaaagtgat tacttccaga actgtttggg atgctaagaa tggtttctga
     4201 gatgttggtt tggaaagtcg taaattagct gttagtggaa aaattaagca ttatgtggtt
     4261 gataatgaca atgttgtgac tcccttgatt cataataatc gtgatattgt tacatttaca
     4321 ggtaattcac gctttaaaca ccgttctcgt ggctattttg aaagtccaat gaatgatatt
     4381 cctaacttta atattggtaa acaagctacc ttggataaac atggttatcg tgatccgaaa
     4441 ttggataaag tgcgattctt taagaaacag gctctacctc aatcttctag tcaaccaagc
     4501 gctgaaccaa tggaaaatat tgccttagga aaacaggtta ctcaaagttc gacagctttc
     4561 ggaggagatg ctagaagagc tgtggatggc aaagtcgatg gtaactatgg tcacaattct
     4621 gtcactcata caaacttcca atctaagcct tggtggcaag tagatttggc taaagaagaa
     4681 accattcgcc aaatcaatat ttacaaccga acagacactg cccaggatag attggcaaac
     4741 tttgatgtca ttcttttaga cagttctggt aaagaaattg agtgaaaacg tataatatct
     4801 cctaaagatg tgtcagcaca aattacgatt aaccataaaa aagcgcgcta tgttcggatt
     4861 gggctagaag gctataatgc cctcagtctt gcagaagttg aagttttccg ctttatagct
     4921 acgaatgctg aaacggcgac acaagtttct aagccagttc aaccaatcag tcagactcct
     4981 gtgaaagata aaacattgac aattcaacac agtggagctt acattgcccg ctactccata
     5041 acttgggaag aagttacagt agataaagat ggaaaccaag ttgttcgtag tcattcttgg
     5101 gaaggaaacg gtcgcaacca gactgcaggt tttgtcctca acctcccaat caaagaaaat
     5161 atgagaaatc tgcgagttaa gattgagaaa aagacgggcc tactatggaa tagatggcaa
     5221 acaatctatg aaaacagacc aattttagct caaccccacc gtaaaattac ccattggggt
     5281 acgacattga attccaaggt gagtgacgat gatgtcttgt aatctgatgg tacaatgaca
     5341 gttagtttgt ctagtttata agaaagtact acctgagctt gaatagaact caggtagctc
     5401 tctgtgaaag aacaaaatta atactcaatg aaaatcaaag agcaaactaa gaaactagcc
     5461 gcaggttgct caaagcactg ctttgaggtt gtagataaga ctgacgaagt cagtcacata
     5521 tataatccaa ggcgacgttg acgtggtttg aagagatttt cgaagagtat aaacagaaag
     5581 gtagagcgcg tgttctaatt tgaacacgag tagaaaactt ttctaaaaat aaaaacgaaa
     5641 ggatgggtaa actgtattcg ctgaactgaa tacgggcgac tctcctctaa atcaaaatta
     5701 agaaagaaag gaattgaccc caccctaaaa gcagtgggaa aaagatagtt ggtctagcga
     5761 gcatcgctca ctgcgcccaa ctcctatttt cccttcgctt tttgatgggt ttggtatctt
     5821 tctcaatata aaatataaaa taaagaaagg tagagcgtgt gttttgattt gaacacgagc
     5881 ggaaaactcg gaaaatagat aatctgactg aaaaatcagg atttctcgtc aggttcctaa
     5941 ttttcagtcg ttttcttctc gctctttgta tcataaatta tgtctatcca tattgctgct
     6001 cagcagggtg aaattgctga taaaattctt cttcctgggg atcctcttcg tgctaagttt
     6061 attgcggaga atttccttga tgatgctgtt tgttttaacg aagtgcgtaa catgtttggt
     6121 tacactggta cttacaaggg tcactgtgta tctgtcatgg gaactgggat gggaatgcca
     6181 tctatttcga tttatgcgcg tgagttaatc gtagactacg gtgtgaagaa attgattcgt
     6241 gtgggaactg caggttcttt gaatgaagag gttcatgttc gtgaattagt tttggcgcag
     6301 gcggctgcaa ccaactcaaa catcgttcgt aatgactggc cacagtacga ttttccacaa
     6361 attgctagct ttgatttgct tgataaagcc taccatatcg ccaaaaaact tggtatgact
     6421 actcacgttg ggaacgtttt gtcatctgat gtcttttact caaattactt tgaaaagaat
     6481 atcgagcttg gtaaatgggg agtcaaggct gtggaaatgg aagcagcagc tctttactat
     6541 cttgctgccc aataccatgt tgatgcgcta gctatcatga ccatctctga tagcttggtc
     6601 aatccagacg aagacacaac tgcagaagaa cgtcaaaata ccttcactga tatgatgaag
     6661 gttggtttgg aaaccttgat tgcagaataa ttatagccaa aaaggaagcc ctttatgaat
     6721 tgtaggactt ccttttctta tccagaaatt gatctagctc tctctgattt cgaagaatag
     6781 tgactttatg tgaatattct tgacaaagtt tttggtaatt ttctttttga gttttgctac
     6841 gcccatccca aagaatccat ctgataaact cccagtcaaa gcgttcaggg caatctgccg
     6901 ccatactttc tctgactttt ccacggtatt taagataacg cttaaaggtt ctaaagagac
     6961 aggtcaatgg cgaaaaattg agaaagatga tttggtcagc ttcttgcatt cgttcttggt
     7021 agcagcacca agaataatta ccatcgatga cccaagcttt atgcttggtg agaaagtttt
     7081 ttatatcggt taacatccat tcgtggtcac tgtcttgcca accaggttga aattggagtg
     7141 tgtccatgtg cagttttgga atggagtagt agttagataa cttttctgct agagttgact
     7201 taccagaacc agaatatccg ataattgcga ttttcatttt ctaccttttc ctatttggag
     7261 acaaaaaaac agcctctatg gactgtttct tatttagcaa gtttagctga aagacgagct
     7321 ttatcgcggc ttgctttgtt tttgtgaatc aaacctttag tttctgcttt atcgatagct
     7381 gagctagcag cacggaaaag ttcttcagat gggtttgctt cgaaagcttt tatagcagta
     7441 cgcatagctg atttttgagc tgagttcttt tcgttttgtt taacgttcaa ttcagcgcgt
     7501 ttgatagctg atttaatgtt tgccaatatt cttacctcca tatttactaa ctataccatt
     7561 atatatgaaa acttacgttt tgacaaggga aaattacttt tttaagtaaa tttcatcgat
     7621 ttcatggttc ttgcttttat gaagaatcac ttctgcacga tttctggttg gttcaatata
     7681 attttgcaga tttgtgagat tgatactgat ccagacctga tgggcaaagg actccacttc
     7741 cccaatcggc atctgagtaa aacgataata gtagctatca gggtcgtttt gggctaggct
     7801 cagcattttc aagaaacggt ccagatacca actttcaata tcatccactc cagcatctac
     7861 atagatggaa aagtcaaaga agtcagtgat atagagacga tcgttttgtg gattttgaaa
     7921 gacattgatt ccctcaacaa ttacaaaatc agcagctttg acactttgtt tctcttcggg
     7981 tacgatgtcg taaacttcat gagaatagac aggaatatct acatcttgtc catttttgat
     8041 gcggtccaag aagttgagaa gagcttccat atcatagctt tcaggaaatc ctttacgatt
     8101 taaaatcccc tgctcaatca aggtttgatt gggatagaga aaaccatcag ttgtaaccaa
     8161 ctcaaccgta gcatctgtaa acgtacggga cagtaggatt tgaagtaggc gactggttgt
     8221 ggattttcca acggcaacac tcccagaaac cccaataata aaaggttgag atttactttc
     8281 acgttggagg aaaattcctt ttgaaaaggc taaatcttcc ttagttcgct tgtaaatctg
     8341 aatcagatga gccaagggga gatagatatc tgtaacgtct tggagactga tttggtcatt
     8401 aaaactcttg atagattcca attcttcttc tgtcaaagga ggtgttgtct ttcgatgtaa
     8461 agattgccaa gtctggcggc tgattttttc aaaatgtaaa aattcgttgg tcatttgttt
     8521 tcccctaggt attttatcta tcataccata aaaagctaaa aaaataaagc ggtttcttga
     8581 atgttgtaag tcaatgatat ataatataag cgatcaattt attgatgaaa taggagatag
     8641 attctactga tttataagct tataaagggg gctttttagt tcacaactta taggaggtgt
     8701 actatgaaaa tcttaaaacg ttacatattg gaactctgtt ttattttaag ttttgcttta
     8761 ccttttataa aaggagccaa tgcagataat ggtagatgct ttgtggaaac ctattacggt
     8821 tttacttttt tgatggaaca tgctattgta acagctgtct ttatctattc gtttttaatt
     8881 gctttcttac taaaaaaacg atgggcgaaa tggattgctg ctggtagtta ttgcttttta
     8941 gtcctatgga ttgcgacaga gggttatttc ttccgcatgt cactagaaga tttgatacga
     9001 ctttggacaa gtttggaaat cctgacacaa acgtatcagt tgggctttta tctaaacatt
     9061 ttgttgggaa ttttgttgat aataaagtat tt
//
LOCUS       DAVMEF010000069         9073 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMEF010000069 DAVMEF010000000
VERSION     DAVMEF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.069"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(52..1266)
                     /gene="thiI"
                     /locus_tag="VYQ30_001211"
     CDS             complement(52..1266)
                     /gene="thiI"
                     /locus_tag="VYQ30_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1966319.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGRAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(1275..2417)
                     /locus_tag="VYQ30_001212"
     CDS             complement(1275..2417)
                     /locus_tag="VYQ30_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1966320.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            2555..2983
                     /locus_tag="VYQ30_001213"
     CDS             2555..2983
                     /locus_tag="VYQ30_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966321.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            complement(3028..5253)
                     /locus_tag="VYQ30_001214"
     CDS             complement(3028..5253)
                     /locus_tag="VYQ30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1966322.1"
                     /translation="MLISLGIAILLIFATFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(5417..7369)
                     /locus_tag="VYQ30_001215"
     CDS             complement(5417..7369)
                     /locus_tag="VYQ30_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1966323.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(7366..8277)
                     /gene="pfkB"
                     /locus_tag="VYQ30_001216"
     CDS             complement(7366..8277)
                     /gene="pfkB"
                     /locus_tag="VYQ30_001216"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1966324.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(8274..9014)
                     /locus_tag="VYQ30_001217"
     CDS             complement(8274..9014)
                     /locus_tag="VYQ30_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1966325.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
BASE COUNT         2590 a         2116 c         1769 g         2598 t
ORIGIN      
        1 aaaaaactaa ctttttttac tgattttcgc tattcttttg gattttctga attagagcag
       61 attgtcaatc aagtcatcaa cttcatcttt ttcggcttga ggtgtgattt cagtaatcat
      121 gattccagcc actgctcgct caaccaagcc ttcaacatcc atacgcgctt cgtactgctc
      181 cgcattctta attttaggat ttgtttttgg acgatctggt gcaaaaatgg tacaacagtc
      241 ttcaaacggt tggattgaaa tgtcaaaggt atcgatttcc tgggcgatgt caatgatttc
      301 caacttgtcc atggtaacca caggacgaat gatgggagtg ttggtaacag cattgatagc
      361 cttcatactt tcaagggttt ggctggctac ttgacctaga ctttccccat tgatgataac
      421 taaaccattt cgtacctcac gaatacggtc agtaatacgc atcataaaac gacgcgtcaa
      481 ggtcataagg taggcttctg gcgctttggc tttgatttcc tcttgaatct ctgtaaaagg
      541 aacttcgata aactggatat ttcccccaaa tttggttaat ttacgggtca aatcttgggc
      601 ttttttaaga gcaccaggac tcgtgtaagg tgggctggca aagtgaactg cctctatatc
      661 tacccctcgt ttaagcgcta gatagccagc tacaggggaa tcgattcccc ctgacaacat
      721 gagcatgccc ttaccagaag ttcccactgg taatccacct gcccctcgaa cggtttcata
      781 agaaagatag gctgcttctt cacgtatctc cacctgaaga ttgatgtcag gacttttcat
      841 ttgagcttgt acgtttggaa tggcttcgaa tacagccctt ccaagcgttt ggttgagttc
      901 acgactgtcc aattcaaagg tgtggtcgct acgcttgcta gagattttaa aggtcatgcc
      961 ttccttgtag atgtcctgca taatctcttg gacagcagac ttcagaactt ctacagattt
     1021 ttcaacctta taaacagggg aaaagttttg aattccaaat acttgtttga gcgattctgc
     1081 tactgctgtg taatctgctc cattgaggta agcgtgggca cggtcgcgat ctgctgttac
     1141 cttaacttgg ggatagatag acaaaacgtc tgaaatatta ttgcgaagtt tattgatgaa
     1201 acgcatacga tttttgccct tggttgacag ctcaccgtag cgaatcataa tttctgaata
     1261 ctgcatgaat gctcctatct tacttttcta gtttgattgt aaattaattt tagcttggtc
     1321 aagaactgct cgacctgact catatcattt tcaaggtcta gactaagacg cacagctgac
     1381 tgagccttat ccttgtccac tcccatagca atcagggttc cggctggttt cccagccttg
     1441 gacgaacaag ccgaggtcgt tgagatgaaa atatcatagt cttcaaaggc gtgaacaatg
     1501 acctcaccac gaacaccctt gattccaaaa gtcagaatat gaggggcgaa gtcttcctca
     1561 tctgaaaaga caaaaatatc cggatagttc agaagagctt ggcgaatcac tgccttcatc
     1621 tgcccagtct tgctcctaaa gatatctagc ttttccatag acaaacggag ggccttggct
     1681 gtcgctgcaa tccctgccac attttcagtt gtcgaacgat aatctcgctc ctggccacca
     1741 cctgtaagaa gaggtgtaat cttcttgcca gatttgatat agacaaagcc aacacctcga
     1801 accccgtgga acttgtgact agagaaagtc gcgcaatcca cccgttctgt cagatacttt
     1861 tcagtcggaa ttttggcaag cgcctgaacc gcatcaacgt ggaaggaaat agtcggcttg
     1921 tctgccaaga attctgaaat agcctcaata ggttggatag agccgatttc attgttcaca
     1981 gccatgatgg aaacgaggat tgtatcatgc cgtatcaaac ctgctaacgc ctcaacatcg
     2041 accaagcctt tcttatcaac tggagcaaaa tccacttcaa atccttgact tttcaaccag
     2101 agggctgact ctttgactgc tggatgttca atggctgaaa caatgatgtg cttgccaaac
     2161 tgagcttttt caaaggccac acccttgata agccagttat ccccttctgt tccacccgag
     2221 gtaaagaaga tttcatcgct tttcttaccg attaaatctg caatctgttg gcgagaagca
     2281 tctaagattc gtgttgcttg gtctcccaaa cgatggagac tagatgggtt tcctaaaatt
     2341 tttgaagcga cctgcatata ggtttcaagg gcttcaggat aaggcttggt cgtcgctgaa
     2401 ttatcaaagt aaatcatgtt tctcacgctt tctaaaatca cttcctctat tgtatcatga
     2461 aacggagctt gcgacaagaa agggaaattt ctcttttttt aagatttttt taaagaaaca
     2521 gagtataata aatcataatc aaaggagaga atgtatgtct aactatcgta gaacttcaaa
     2581 accaaaaaca gaacacatca aaaaaggctt tacagtcttt caaaaaaccg ttgctacaat
     2641 cgctagtatc cttggcttga ttaccgcaag tatcacgatt atgaacgcct tggataataa
     2701 caaaaatatt aaaaaagaac ctacgacaag ccagacgaca accattgtca aagaaatcca
     2761 aaaggaatcc cctaaggaaa acaccagtcc aactaaagag actaacacta gtcaagaaaa
     2821 aacacagcaa gaggaaacac caaaatctag cgtcaaggaa gagaaaaaag aagatcagaa
     2881 aacagcaact caggactctt ctactcctgc ttcaagtaaa cctgctactg aaaacgaaaa
     2941 acagtccaat gcgccaactt cagaaaataa aagcaatcaa tagccactaa aatagcttcc
     3001 ctccaaactt ggaaagaagc tattttttta ttgttgtaac acttttcgag gtttggtacc
     3061 ttcagctgga ccgatgacac ctgctatctc cagttcttcc atgagacggg tcgcacggtt
     3121 aaatccaact gataaacgac gctgaatcat agacgcactg gctttctgtg tttcgataac
     3181 caaagactta gcttcttcaa aaagcggatc accaccagca tctccatccg aaaattctcc
     3241 ttcattttca gaaacctcac ctggatcaaa actctcatcg tagtctgcat ctgcctgagt
     3301 cttgatgaag ttcacaatgc gctcaacatc gtcatccgag ataaaggagc cttggagacg
     3361 aactggatga ttttcatcaa tcggtttaaa gagcatgtct cctcgaccaa gaagtttttc
     3421 tgctccattt tcatccaaaa tcgtacggga gtctgttcct gatgaaaccg caaatgctac
     3481 acgagatgga acattggcct taatcaaacc agagatgaca tcaacagatg gacgctgagt
     3541 tgcaagaatc atgtggatac ctgcagcacg cgccttctgc ccaagacgga tgatagcatc
     3601 ttccacttcc ttgctggcca ccatcatgag gtcagccaac tcatccacaa tcacgacaat
     3661 gaatggtagc ggaatttgct tgtactcaga ctgggaattg aactcttcta ccttggcatt
     3721 aaaacctgca atattccgaa ctcccacctt ggcaaagagt tcataacggt tttccatttc
     3781 atccacaacc ttttgcagag ccttgctggc tttgcgtgga ttggtcacga ctggaatcaa
     3841 gaggtgggga atatcattgt aaacagataa ctcaaccatc ttgggatcga ccatcataaa
     3901 tttaacttga tctggtctcg ccttcatgag aatgctagca ataatgccgt taactgctac
     3961 tgacttccct gaacccgttg aacctgcaac tagcaagtgg ggcattttag aaaggtcaaa
     4021 agctcttgcg gttccattaa cagccttccc taaaggaatt tccaagaaat tttctgcttt
     4081 cgtttgcgat tgttcccata gttctcggaa agatacagtg gcaatatcgg agttgggcac
     4141 ttcaattccg attagggatt tcccagggat tggtgcttca atccggacat ctttggcagc
     4201 caaggctaga gcgaggtcat ctgatagatt ggaaatgcgg ttgaccctta caccaacagc
     4261 cggcttgact tcatacttgg tcactgatgg cccaatttcg gcccgttcaa ctgttacctt
     4321 aataccaaag ctagcaaagg ttgcttctaa gattttgata ttttctctga caattttctt
     4381 ctctttagac tgatcttttg gtttatctgg tgcaaagagt tgtaagcttg gaagtttgta
     4441 ttcaagggct tctttggctg aaaaatcgac ctgaacatct tcgtcatctg agtcatcttc
     4501 ctgttcaggg aatttaagtt cagcttgagg caggatgatt tctggttcca cccacttttc
     4561 ttctggaata ggtggaagat tttgaacagc ttcctctgtc agaatttcac ccgtttccat
     4621 atcaacagga ggcaaatcga gtaaggcttt ttcagtctct tcttgttcta atctagcctc
     4681 tttctcagcc ttttggcgag ctttttcttc ttgtttgaca aagcgttcct cttttcgacg
     4741 ctcgtgccct tcccaccatt tagcaaagcc tctactgaaa aattcagcaa tatcgtaaac
     4801 agaccaaggg ctgactagga gagaacccac taaaatcaag atagaaccaa taaagtaagt
     4861 tccgatattt gaaaagagaa aggctgttgg aatataaaga gcgaccccga tcaagccccc
     4921 tccagcaaaa ctagtcgttc gaaaaccagt cagatctgtc acaacctgag ccatggtccc
     4981 ttttagaacg gacttgtcca aaccatattt ccaaaccaag taggcttcaa aaatcaagag
     5041 taagccagca aatatggtga aaaagccaga taagagtcct tcctgttttc gtatccactt
     5101 gaaaaaaaag agatagatta ataggccgaa tatcgccaga taagctaggc tacccactag
     5161 caagcgaatt aaattataaa gggttatacc tgcagcccct aatttgaagg ttgcgaaaat
     5221 caataaaatc gcaattccta atgaaatcaa cattcgttga atcgcttctt ttctttccag
     5281 ttctgcttta gacggtctcc gtcttgttgt acttgtattc ttgtttgcca ttcttctatt
     5341 ataccatatt tcacaggcat ttcgggaaga gaaaaagact gcaccaaacg gtccaatctt
     5401 ttcatttttc tattttttat gcttgtggtt tgcgtaggta accataaacc acaccactta
     5461 cgattgctcc taccaagaca gaaacgaggt aaaggagagc atttgaagta agggcgataa
     5521 cgaagattcc tccgtgtggc gccatgagtt tgataccagt aagaccaacg agtccacctg
     5581 ctactgctga accaaggatg aagcttggaa tcgcacgagc tgggtcagcg gcaccaaatg
     5641 gaatcgctcc ctcagtgata aatgacaagc ccatgatgat gtttgtcaaa ccagagttac
     5701 gttcttcctt agtaaattta tctttgaaaa gaagagttgc gacaaagatt gcaagtggtg
     5761 gcaccattcc tccagccata actgctgcca tggctacaga accacctgaa gaaacagttg
     5821 ctgcaagcgt acctgtacca aagacataag ctgctttatt aactggtcca cccatgtcaa
     5881 cagccatcat tccaccaagg acgataccaa gaaggacagc tgaacctcct ccaagaccgc
     5941 ctaggaagtc attcatagca gtgttgattg cagccattgg gatattcaca gctagcataa
     6001 caaatcctgt caagattgtt ccaagaagtg gcaatagaag gattgattta gcaccttcga
     6061 gtgaacgagg aactttaacg tatttcttga tggcaagaac caaggcacct gcgataaatc
     6121 caccaacaag ggcacctagg aaaccagatg agacacctgc aagagttgaa gttgcttcac
     6181 cacctgcggc ataaggaatt ttaccaaagg caaaaccttc tttggcaata gcaccagcca
     6241 cgaaacctgc taccaaaccc ggtttttcag caatagagta ggcaacataa cccgcaaaga
     6301 ctggaagcat caaaccaaag gcagctccac caattttcat gaacatagaa gctaactcat
     6361 ggtaagaacc aagattgcca aggttttcat ttggaacacc caaagcaccg tcaatcaaga
     6421 aggcaagggc aatcatgata ccaccaccga taacgaatgg taacatttga gatacaccac
     6481 tcatcaagtg tttgtacaag gcaccaccaa ggctttgttt ttcgttagag gctgttgcag
     6541 ctttggcacc attagcggca cggtagactt cagtatctcc tgaaagagcc aagttaatta
     6601 gctcttctgt cttacggata ccgtcagcaa ctggacgatt gatcaatggt tttccatcaa
     6661 atcgatccat ttcaacggcc ttgtctgctg caatgataat agctttagcc ttacggatat
     6721 cttctgcagt tagttgattt ccaacaccgc tagcaccgtt ggtttcgacc ttgataccaa
     6781 cccccatttc agcagctact ttttgaaggg cttcttgggc catgtaagtg tgggcaatac
     6841 ctgttgtaca agctgtaaca gctacgataa agtcaccaga gtcattagca ggtgcttgaa
     6901 caagttcctc agttttttct gaagcttggt caaaaagttc gataacttgg cctgcagatg
     6961 ttgcttgacg aagtttgtct gcaaaaccgt ctttcatcaa gtattgagac aattctgcca
     7021 aggctgccaa gtgagtatca ttggcacctt ctggagctgc aatcatgaag aagaggtcag
     7081 ttgcttgtcc atccaagctc tcgtagtcaa cacccttgtt tgacttagca aatagaactg
     7141 tcgcttcttt gacagcagcg tttttgctgt gaggcattgc gattccatca cccaaaccag
     7201 tagaagtcaa agcttcacgc gccaaaattc cttctttaaa tgtttcaaaa tctgttacat
     7261 aaccgtgctc tgtcaaattt ttaatcatct cgtcgatgac agctgttttt tcagttgcct
     7321 gcaaatctag caacatgaca tcttttctca ataggtcttg aattttcatc ttttttctac
     7381 ctcaactttt ccatatgttt ctttaataaa ttccgccgtt gccaagtcat ctgagaaggt
     7441 agttgccgtt ccgcaagcca ctccccattt gaaggcttct actgcgtctt ttgatttgac
     7501 aaattcacct gtgaatccag caaccataga atcaccagct ccaactgaat ttttgactgt
     7561 tcctttgatt ggtttagcga agtaagctcc ctcagatgtg acaagaaggg cgccatcacc
     7621 agccatagag ataataacat tttgagcacc cttagccagt aactcacgag cgtatttctc
     7681 aatttcatct aaactttcga gtttaacccc aaaaatcgct ccaagttcat gattgtttgg
     7741 ttttacaaga agaggctggt agtccaaact atcaattaag gtctgtcctt caaagtcaca
     7801 gaccacttgc gcaccagtct ggcgcgtcaa ggaaatcaaa tccttataga taacattgcc
     7861 tagattttta gcacttgaac ctgcaaagac aactgtatct tctactgtca gactagataa
     7921 aatagctttc aattcttcta gctgaaccgg ttcaacagtt ggacccgttc cgttgatttc
     7981 tgtttcttgg tctgctttga ttttaacatt gatacgagta tcttctgcca cttggacaaa
     8041 acgtgtctcg atttcttcct ctgctagagt atctgtgata aatttaccag taaagccacc
     8101 gataaatccc gtcgctgtat ttggtatatt caaacgtttc aagacacggc tgacattgat
     8161 tcctttccca ccagcaaact tatcatcact gtccatacga ttgacactac caactttgac
     8221 ttggtccaaa cgaacgatat agtcaatgga tggattgagt gtgactgtat aaatcatact
     8281 tctattacct ccgttttctc cttaataacc tgcaagagct catgcccttg actagtgata
     8341 acgatagcgc gtttgagtgg tgctaccttg gcaaagcaag tttgtccaat ttttgacgaa
     8401 tccaccaaga cgtaggtctg cttggcattc tccaaaatag ctcttttcac agctccctcc
     8461 tccatatcag gagtcgtata atagccatcg tcaacaccat tcattccgat aaaggcacgg
     8521 tcaaagtgca attggttaat ctggttaaga gcaacgcccc cgatactagc atctgtcgcc
     8581 gtcttgacgt ttcctccaac catgacagtt ggaatctgct tttcaaccaa ctgagcggca
     8641 tggtgaatgg agttggtcac aactgtaaca ttcttattga ccaattcatg aatcaaaaaa
     8701 gcagttgttg ttccagcatc gataaagatg acatcttttt ctttaatgag agaggctgct
     8761 ttctgagcca gcaacttctt ttcttgaagg tttttgacag atttttcttg aatggtttct
     8821 tcttcctgta aggagtgggg gagttctgct ccaccatgca cacgacgaag cttgttttcc
     8881 gcttccaact catccaagtc tcttcgaacc gttgattctg acgtttctag caaactaact
     8941 aatttttcta gagaaactac atgatgctga tttaactcct ctaaaattag ttgtttacgt
     9001 tctgttttta acactgaatc acctcctgtt atcgtttaca ctattcattc tatcatactt
     9061 tctttcgaag tca
//
LOCUS       DAVMEF010000070         9066 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMEF010000070 DAVMEF010000000
VERSION     DAVMEF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.070"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            245..2032
                     /locus_tag="VYQ30_001218"
     CDS             245..2032
                     /locus_tag="VYQ30_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1966326.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2029..2505
                     /locus_tag="VYQ30_001219"
     CDS             2029..2505
                     /locus_tag="VYQ30_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1966327.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2533..3438
                     /locus_tag="VYQ30_001220"
     CDS             2533..3438
                     /locus_tag="VYQ30_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1966328.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3425..4240
                     /locus_tag="VYQ30_001221"
     CDS             3425..4240
                     /locus_tag="VYQ30_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1966329.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4247..4651
                     /locus_tag="VYQ30_001222"
     CDS             4247..4651
                     /locus_tag="VYQ30_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1966330.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4945..6111
                     /locus_tag="VYQ30_001223"
     CDS             4945..6111
                     /locus_tag="VYQ30_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1966331.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6227..7264
                     /locus_tag="VYQ30_001224"
     CDS             6227..7264
                     /locus_tag="VYQ30_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1966332.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTYGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7471..8325
                     /locus_tag="VYQ30_001225"
     CDS             7471..8325
                     /locus_tag="VYQ30_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1966333.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8475..8732
                     /locus_tag="VYQ30_001226"
     CDS             8475..8732
                     /locus_tag="VYQ30_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1966334.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         2581 a         1702 c         2053 g         2730 t
ORIGIN      
        1 gcgatatagg gattgctcat attttatcaa tgtattctat acaaaattat tttaggtcct
       61 atattccgaa tttaaagagc gtttgaaatg ggtctaaaaa tgtttgaaac taattgaatg
      121 tgtgtataat agtaaaaatg cagtaaatat cccgacaatt tctattgaca attcaaacag
      181 attggtttat aattaatata acaacaaatg aaagcgcaaa ctttcgcggt cggaaggtag
      241 ttttatgaca cgatttgaga tacgagatga tttctatctc gatggaaaat catttaagat
      301 tttatctggt gccattcatt attttagggt tcctccagag gattggtatc attcgctcta
      361 taacttgaag gctcttggtt ttaatacggt agagacttat gttgcttgga atttacacga
      421 gcctcgtgaa ggtgagtttc attttgaagg tgatctggat ttagagaaat ttctccaaat
      481 agcgcaggat ttgggtctct acgcaattgt gcgtccgtct ccatttatct gtgcggaatg
      541 ggaattcggt ggcttaccag cttggctctt gaccaagaac atgcgaattc gctcatccga
      601 cccagcatat atcgaggcag ttggtcgcta ctatgatcag ttattgccaa gactggtgcc
      661 tcgtttgttg gacaatggtg gcaatattct catgatgcag gttgaaaatg agtatggttc
      721 ttacggagaa gataaggctt acctgagagc gattcgacag ctaatggaag agtgtggcgt
      781 aacctgtccc ctctttacat cagatggtcc atggcgagct actctgaaag ctggaacctt
      841 aattgaagag gacctctttg taacaggaaa ctttggttct aaggcacctt acaacttttc
      901 gcagatgcag gaattctttg atgaacatgg taagaaatgg ccactcatgt gtatggagtt
      961 ctgggatggt tggttcaatc gctggaaaga accgattatc acacgggatc ctaaggaatt
     1021 ggcagatgca gttcgagagg ttttggaaca aggctctatc aatctttaca tgttccacgg
     1081 tggtacaaac tttggtttca tgaatggttg ctcggctcga ggaactttgg acctgccaca
     1141 agttacgtct tatgattacg atgcccttct ggatgaagaa ggaaatccaa ctgctaaata
     1201 tcttgcagtc aagaagatga tggcaacaca tttttcagag tatccgcagt tggaaccact
     1261 ctacaaagag agtatggagt tggatgctat tccactagtt gaaaaagttt ctttatttga
     1321 aaccttagat agcttgtcaa gtcctgtaga aagtctctat cctcaaaaga tggaggagct
     1381 gggacaaagt tatggctacc tactttatcg aacagaaaca aactgggatg cagaagaaga
     1441 aagacttcgt atcattgatg gtcgagatag ggcccagctg tatgtagatg gtcagtgggt
     1501 taaaactcaa tatcagacag agattgggga agatattttt tatcaaggta aaaagaaagg
     1561 gctatctagg ttagatatct tgatagaaaa tatggggcgt gtcaactatg ggcataagtt
     1621 cttagcggat acgcaacgta agggaattcg gacaggggtc tgtaaggatc tgcatttctt
     1681 actaaactgg aaacactatc cactcccact agacaatcct gagaaaattg atttttcaaa
     1741 aggatggact caaggacaac cagcctttta cgcttatgac tttacagtcg aagagccaaa
     1801 agatacttac ctagacttgt ctgagtttgg taagggggtt gcctttgtca atgggcagaa
     1861 tctaggacgt ttttggaacg ttggcccaac tctctcactt tatatccctc atagctatct
     1921 caaggaaggt gccaaccgca tcattatctt tgaaacagaa ggtcaatata aagaagagat
     1981 tcatttaact cgtaaaccta cactaaaaca tataaagggg gaaaacttat gacaattgta
     2041 ggatgccgta ttgatggacg tttgatccac ggacaagtag ccaatctttg ggctggaaaa
     2101 ctaaatgttt cacgcattat ggttgtagac gacgaagttg tcaacaacga tattgaaaag
     2161 agtggtttga aacttgcgac accaccaggt gtgaaattga gtattttgcc agttgagaaa
     2221 gctgcagcca atattcttgc tggcaaatac gatagccaac gtctctttat cgtggctcgt
     2281 aaaccagacc gcttccttgg tttggtagaa gcaggtgtac cacttgaaac ccttaatgtt
     2341 gggaatatgt ctcaaacacc agaaactcgt tctattacac gttctatcaa cgtagtagac
     2401 aaggatgtgg aagacttcca caaactggca gaaaaaggtg ttaaacttac tgctcagatg
     2461 gttccaaatg atccaatttc agactttttg agcttattaa aataggaaaa aaatttttag
     2521 gaggtcattg ttatgataca atggtggcaa attttacttc tcactttgta ctcagcttat
     2581 caaatctgtg atgagttgac gatcgtttca tctgcaggtt cccctgtatt tgctggtttc
     2641 attactggtt taatcatggg agatgtgact actggtttac ttatcggtgg taacttgcaa
     2701 ctgttcgttc ttggggttgg tactttcggt ggtgcttctc gtatcgacgc aacttctggt
     2761 gcggttcttg cgacagcctt ctctgtttca caaggaattg atgcaccgct tgccattact
     2821 acaatcgctg taccagtagc agctctcttg acttacttcg acgttcttgg tcgtatgact
     2881 actaccttct tcgctcaccg tgtggatgct gcaatcgaac gctttgacta taaaggtatt
     2941 gaacgcaact acttgcttgg tgcgattccg tgggctctat ctcgtgccct tccagtcttc
     3001 tttgcccttg cttttggtgg tgcctttgta caatcagtag tagacttcgt tgaagcctac
     3061 aaatgggttg cagatggctt gacacttgca ggacgtatgc ttccaggtct tggatttgca
     3121 atcttgcttc gttaccttcc agttaaacgt aaccttcact accttgctat gggatttggt
     3181 ttgacagcta tgttgactgt tctttactca tatgtaacag gtcttggtgg cgctgttgct
     3241 ggtatcgtag gtactcttcc tgctgaagtt gctgaaaaaa ttggtttcgt gaacaacttc
     3301 aaaggtttgt ctatgattgg tatttctatc gtaggtattt tccttgcagt gcttcacttc
     3361 aaaaatagcc aaaaagtagc tgtagcagca ccttctacac catcagaaag tggggaaatc
     3421 gaagatgacg aattctaatt acaaacttac aaaagaagat tttaatcaaa tcaacaaacg
     3481 tagcttgttt actttccaat taggttggaa ctacgaacgt atgcaagctt ctggttacct
     3541 ttacatgatc ttgcctcagt tgcgtaaaat gtatggtgat ggaactcctg aattgaaaga
     3601 aatgatgaaa gttcatactc aattcttcaa tacttcacca ttcttccata ccattatcgc
     3661 tggttttgac cttgccatgg aagaaaaaga tggtgtaggt tcaaaagacg ccgttaacgg
     3721 tatcaagaca ggtttgatgg gaccattcgc tcctcttggg gatacaatct ttggttcact
     3781 tgtacctgct atcatggggt cagtcgcagc aactatggct atcgctggcc aaccttgggg
     3841 gatcttcctt tggattgcag ttgcagtagc gtatgacatc ttccgttgga aacagttgga
     3901 atttgcttac aaagaagggg ttaaccttat caacaacatg caaagtacct tgacagcttt
     3961 gattgacgct gcatctgtac ttggtgtctt catgatgggt gctcttgtag caacagtgat
     4021 taactttgaa atttcttaca agttgccaat cggtgaaaag atgattgatt tccaagacat
     4081 cttgaaccaa atcttcccac gtttgcttcc agcaatcttt actgccttta tcttctggtt
     4141 gcttggtaaa aaaggtatga actctactaa agctatcggt attattatcg tacttgcttt
     4201 ggctctttct gcccttggtc actttgcact tggaatgtaa ttccttatga ctaaatcatt
     4261 aattttggtg agccatggtc gcttctgtga ggagcttaga ggtagcacag aaatgattat
     4321 gggcccacaa gacaacattt acacagtagc tcttcttcca gaagatggcc cagaagaatt
     4381 tactgctaaa tttgaagctg ttattgaagg gttggatgat ttcctagtct ttgcggatct
     4441 tctcggtggg acaccttgta atgtggtgag tcgcttgatc atggaaggtc gtgatattga
     4501 cctttacgca gggatgaatc ttccaatggt gattgaattt atcaatgcga gccttacagg
     4561 cgcagatgcg gactacaaga gccgtgctgc agaaagcatt gtgaaagtta atgacctgtt
     4621 agcgggcttc gatgatgacg aagatgaata atactcttcg aaaatctctt caaactacgt
     4681 caacgtcgcc ttgccgtagg tatatgttac tgacttcgtc agtcttatcc ggcaacctca
     4741 aaacggtgtt ttgagctgac ttcgtcagtt ctatctgcaa cctcaaagca gtgctttgag
     4801 cagcctgcgg ctagtttcct attttagttg gaactcgatt caattcatgt gacaacgtga
     4861 aaatcgttag agcattttat atagaatata catgggaatg tagcttactc ccattcccat
     4921 atttaataga aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg
     4981 ggtgcagaaa tcactacgcg tgaaatctac caacagcctg atgtatggag agaagctttt
     5041 gaattttatc aagcaaaacg tgaagaaatt gcagcctttc tacaagaaat cgctgataaa
     5101 catgactata ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagatacc
     5161 ttgctacctt actttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg
     5221 acaacagata tcgttgccaa accagcaacc tatttgaaaa aagatgtggc aactgtcctt
     5281 gtgtcttttg ctcgtagtgg gaactcgcct gaaagtgtgg cgactgttga tttggccaaa
     5341 tccttggtgg atgagcttta tcaagtgacg attacttgtg cagcagatgg taaattggct
     5401 cttcaagctc acggtgatga ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat
     5461 gctggatttg ccatgacttc tagctttacg tctatgatgt tgacagctct cttggtcttt
     5521 gatcctacag aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt
     5581 aaagttttag acaaggcaga agatgtcaaa gagctcgttg atttagactt taaccgtgtc
     5641 atctatctag gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg
     5701 gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt
     5761 ccaaaatctc ttatcaacga caatacagtt gttttggtct ttggtacaac gacagactac
     5821 actcgtaagt acgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt
     5881 gttgtgcttt tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggcccttggt
     5941 tgtggcggtg tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc
     6001 tttgccctat tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt
     6061 acagtaaacc gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt
     6121 tatgaattct tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc
     6181 tttctatggt ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac
     6241 agagcgtgta tttggaaatg ttgagggcga ggatgtcttg gcctatcgat ttgagacaga
     6301 cggtggctac caacttgagg ttatgactta tggtgcgacc atcttgcgct atgtcgcacc
     6361 tgacaaggct ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt
     6421 aggcaatagt cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc
     6481 gacctttgag ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa
     6541 tcacagtggt tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca
     6601 tggcttgact ctctacacag agcgtacata tgggacagga gggttccctg gaaatctcaa
     6661 gatttggatc agttatcact tggaagaaac tggtgcctat gaaatcagct acaaggtaac
     6721 gaccgatcag gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga
     6781 tttcacgcag acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat
     6841 cgctcctgac ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacat
     6901 ctacaatggt gccttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc
     6961 atcaggtttg gatcatccat ttgcccttcc tgcaggccat gacaatgctg gattccttta
     7021 tgaccaaaat tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta
     7081 cacagcaaac tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa
     7141 tgggattgct cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca
     7201 agtcattctt aaagctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa
     7261 ataaaaaagc tggtcaaaaa gtctttaaaa ttaaaataac gcatattgtc aggtgatgaa
     7321 aagtttgata ctatgcgttt tattatgaaa tgatttactt tatattatcc tgaaatggtg
     7381 ttatttttga gttatctggc ttgactttct tgatggaaat tatataatag ttgtaataac
     7441 aataattaca acgagaaaag aggaccattt atgacctatg aatacaagag tcacatttat
     7501 ttggcagaga cagttttaaa tgtaaaggat ttggccagtc aaacagcctt ttatcagcaa
     7561 gttattggtt tagaaatctt atctcaaact gagacagagt ccattctagg ccttggtgga
     7621 aaagtcttgg tacagctgat tcaagcacaa gagagcggag aagtgaggga acattatggt
     7681 ctttaccatt tggccattct cttgcccaca cgcaaggctt tggctgatgt tttgaaacac
     7741 ctgacagatt tacaaattcc tcttgtcggc ggtgcagacc atggttacag tgaagctatt
     7801 tacttggaag atttggaggg aaatggcatt gaactctatc gagataagcc agtttccaca
     7861 tgggatattc gagaagatgg acgcattatc ggagtgacgg aggctcttgc ggcgcaggac
     7921 atttatgagt tgggggaaag agtagagccc tttatcctgg cagaaggtac gagaatgggg
     7981 catattcatc tttccgtcaa ggatagtcga aagtccagcc agttttatca aaaggtgtta
     8041 gggctagagg ataaattcag tgtgcctagc gctagttgga ttgcggctgg agattaccat
     8101 catcatttag cagttaacga atggggagga aaaggtctgg cttcgcgtaa gcaaggctta
     8161 ccaggtttag cctactatgt catcgaagtc gcacataaaa aagaactgtt aacgattgcc
     8221 caacgagcac aagaagttga cgcaccaatc aaatggatga catcgagcca attggaaatc
     8281 acagactcag atggcatcgt gacccgtatt cgtttagcaa ggtaaaatta ggaattttta
     8341 ataagttata agtattgcta gtttgctttt tgaacggtct ttggtaacag aactgcgtga
     8401 gctggatggg cgagaatttt gacaaagaag ctgattagag atataatgaa gaaaaatcga
     8461 gcaaggaaaa agaaatgaca aattcaaagt atattacttg tttgaaacgt tcagagggcc
     8521 agttgtgtgg gattcaaaag atgattgaag gagatcgtga ctgtgctgat attgtgacgc
     8581 aactgacagc agtgagatct agtgtagagc gcgtgattga gatgataatt accgaaaatc
     8641 ttactgaatg tatcaatcag ccactagatg attctgaagc tcaaaaggaa cgcctagaaa
     8701 aggctatccg atacttgatt aaacggaaat aaatgaatgt tttgagacga tatgtgcaaa
     8761 ggtctattaa ctattagaat agtttaatgt gagtcaaaag aagttgtaaa aaatctttaa
     8821 aattaaaaaa acgcatagta tcaggtgttg aatgtattga ccccaaaaag ttagattttt
     8881 tctgtctaac ttttggggtg cagttcataa gaaccttggt aatatgcgtt ttattatgta
     8941 gagacttata ctcttcgaaa atctcttcaa accaggtcag cttcaccttg ccgtagatat
     9001 ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     9061 cagttc
//
LOCUS       DAVMEF010000071         9056 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMEF010000071 DAVMEF010000000
VERSION     DAVMEF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.071"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            183..860
                     /locus_tag="VYQ30_001227"
     CDS             183..860
                     /locus_tag="VYQ30_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1966335.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            847..1644
                     /locus_tag="VYQ30_001228"
     CDS             847..1644
                     /locus_tag="VYQ30_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1966336.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            1661..2764
                     /locus_tag="VYQ30_001229"
     CDS             1661..2764
                     /locus_tag="VYQ30_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1966337.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSLGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            3020..4039
                     /gene="galE"
                     /locus_tag="VYQ30_001230"
     CDS             3020..4039
                     /gene="galE"
                     /locus_tag="VYQ30_001230"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1966338.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4041..5006
                     /locus_tag="VYQ30_001231"
     CDS             4041..5006
                     /locus_tag="VYQ30_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1966339.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(5057..5263)
                     /locus_tag="VYQ30_001232"
     CDS             complement(5057..5263)
                     /locus_tag="VYQ30_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1966340.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5315..5791
                     /locus_tag="VYQ30_001233"
     CDS             5315..5791
                     /locus_tag="VYQ30_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1966341.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            5800..6471
                     /locus_tag="VYQ30_001234"
     CDS             5800..6471
                     /locus_tag="VYQ30_001234"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1966342.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            6602..6940
                     /locus_tag="VYQ30_001235"
     CDS             6602..6940
                     /locus_tag="VYQ30_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1966343.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            6943..7572
                     /locus_tag="VYQ30_001236"
     CDS             6943..7572
                     /locus_tag="VYQ30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1966344.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            7812..8963
                     /locus_tag="VYQ30_001237"
     CDS             7812..8963
                     /locus_tag="VYQ30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1966345.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAEEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
BASE COUNT         2564 a         1690 c         2112 g         2690 t
ORIGIN      
        1 aataaactgt ttgtttttta ttttttgaca tttttgcaaa ttgtttaaaa tctttcaatt
       61 ggattcactt attactttag gacattgaaa cttctttcgc cagaggtgct tatattactg
      121 ggaagttgaa gattgaaatg tggtataata agaggtaata gagttttgga aagtgagaga
      181 agatgatttc aaagagatta gaattggtag cttcctttgt gtcacagggg gctattttac
      241 tagatgtggg aagtgaccat gcttatctgc ctatcgagtt ggttgagaga ggccaaatca
      301 aaagcgctat tgcaggtgag gtggtggaag gtccctatca gtctgcggtt aaaaatgttg
      361 aggctcacgg cctaaaggag aaaatccaag tccgtttagc caatggcttg gcagcttttg
      421 aagagactga ccaagtgtct gtcattacca ttgctggcat gggtggtcgt ttgattgcta
      481 ggattttaga agaaggtttg gggaagttag ctaatgtaga gcgtttgatc ctccagccca
      541 ataatcgtga agacgacttg cgtatctggc tacaggatca tggattccag attgtagcag
      601 aaagcatctt agaagaagct ggaaagtttt atgagatttt ggtggtggaa gcaggacaaa
      661 tgaagctatc agccagtgat gttcgctttg gtcccttctt gtccaaagaa gtcagtccag
      721 tatttgtcca aaaatggcaa aaagaagctg agaagctaga gttcgccctc ggacaaatcc
      781 cagaaaaaaa tctggaagaa cgtcaagttc tagtagataa gattcaagct atcaaggagg
      841 tgctccatgt tagcaagtga agtgatccaa gcttatgaag ccttttgccc tcaggaattt
      901 tctatggagg gagacagccg tggtctgcaa attggcactt tagacaaggg tatccaaagg
      961 gtcatggttg ccctcgatat tcgtgaagag acagtggcag aggccattga aaagggtgtg
     1021 gacttgatta tcgtcaagca cgcgcctatc tttcgtccta tcaaggactt gcttgccagc
     1081 cgtccgcaaa atcagattta catcgacctg attaagcatg atatcgcagt ttatgtcagc
     1141 cataccaata ttgatatcgt tgaaaatggg ctcaatgact ggttttgtca gatgctagga
     1201 atcgaggaga cgacttatct gcaggaaaca ggtccagaac gtggaattgg acgtattggg
     1261 aatattcagc ctcagacatt ttgggaattg gcccaacagg tcaagcaagt ctttgaccta
     1321 gatagccttc gaatggtgca ttatcaagag gatgatttgc agaagcctat ttcaagagta
     1381 gcaatttgtg gtggaagcgg gcagtctttc tataaggatg ctttggcaaa gggggcagat
     1441 gtctatatca ctggtgatat ctactaccat actgctcagg atatgctgtc tgatggcttg
     1501 ttagcattgg atccaggtca ctatatcgaa gtgctttttg tggaaaaaat cgcaacactc
     1561 cttactcaat ggaaagagaa aaaaggctgg gaactagaga ttttacctag tcaagcatcg
     1621 accaatcctt tccgtcatat ctagttagaa agtgaagaca atgaaaaaag ttgccattat
     1681 tggagcagga attgtgggag caacagctgc ctactacctc tcgagagaaa gtgacctaga
     1741 ggtgaccgtt tttgaccatg agcaaggtca agccaccaag gccgcagcag gaattatcag
     1801 tccttggttt tccaaacgcc gtaataaagc ctggtacaag atggcgcgct tgggggctga
     1861 tttttatgtg gatttattag ctgatttaga gaaatcagga caagaaatcg acttttacca
     1921 gcgttcggga gtctttctct tgaaaaagga tgaatccaag ttggaagaac tctatcaact
     1981 ggctctccag cgcagagaag aatctccctt gataggccag ttagccattc tggatcaagc
     2041 atcagctaat gaattattcc ctggcttgca gggattcgac aacttgctct atgcttctgg
     2101 tggagcgaga gtagatggtc acctcttagt aactcgtttg ctggaagcca gtcaggtcaa
     2161 gttggtcaaa gaaaaagtga ctctgacact cttatcatta ggctaccaga ttggcgaaga
     2221 ggtgtttgat caggttattt tggcaacggg agcttggttg ggggacatgt tagaaccttt
     2281 aggttatgaa gtggatgtcc gtcctcaaaa aggacaacta cgagattatc agcttgccca
     2341 agacatggaa gattaccctg ttgtcatgcc agaaggggag tgggatttga ttccctttgc
     2401 aggtgggaaa ttatccttgg gcgctaccca tgaaaatgac atgggatttg acttgacggt
     2461 agatgaaccc ttgctccaac aaatggagga ggcggccttg cctcattacc cagttttagc
     2521 tagagtgact tcaaaatctg agcgtgttgg aatccgtgcc tacaccagtg atttctctcc
     2581 tttctttggg caggtgcctg acttaactgg tgtctatgca gccagtggac taggttcatc
     2641 aggcctcaca actggtccta ttattggtta ccatttagct caactgatcc aagacaagga
     2701 gttgaccttg gaccctgaaa actacccaat tgaaaactat gtcaaacgag taaaaagcga
     2761 ataatactca atgaaaatca aagaacaaac taggtaacta gccgcgggtt actcaaagca
     2821 ctgctttgag gttgtggata gaactgatga agtcagtaac catacccatg gcaaggcgac
     2881 gttgacgtgg tttgaattgg attttcgaag agtataagca ttttactgaa attttatcag
     2941 gtagtttagg atggcaaatg acattccctg tcaaaaatgg taaaataaga aaaaataatc
     3001 tgagaatcga ggaaaaaaga tgcaagaaaa gattttggta actggtggtg ccggttttat
     3061 tggaacccac actgttattg aattgatcca agcaggccat caagttgttg tggtggataa
     3121 tcttgtcaat agcaatcgta agagtttaga agttgttgaa agaatcacag gagttgagat
     3181 tcctttctat gaggcagata ttcgtgacac agataccctc agagatattt tcaagcagga
     3241 agaaccgact ggtatcattc actttgctgg tttgaaggct gttggcgaat caacacgtat
     3301 ccctcttgcc tactatgaca acaatatcgc tggaactgtc agccttttga aagccatgga
     3361 agaaaacaac tgtaaaaaca tcatcttcag ttcttctgcg acagtttacg gggatccgca
     3421 cacagtgccc atcttggaag atttcccact ttcagtgacc aacccatacg gtcgtactaa
     3481 gctcatgcta gaggaaattt tgactgatat ttacaaagca gactcagaat ggaatgttgt
     3541 cttgcttcgt tactttaacc caatcggagc ccatgagagt ggtgatttgg gagaaaatcc
     3601 aaacggtatt ccaaacaatc tcttgccata tgtgactcaa gtagccgttg gaaaattaga
     3661 gcaagtgcaa gtgtttggag acgattacga tacggaagat ggaacaggtg ttcgtgacta
     3721 tatccacgtt gtcgatttgg ctaagggtca cgttgcagct ttgaaaaaaa tccaaaaagg
     3781 ttcaggacta aacgtttata accttggaac tggtaaaggt tactcagttc ttgaaattat
     3841 ccaaaacatg gaaaaagcgg tgggccgtcc tattccttac cgcatcgtag aacgtcgccc
     3901 aggtgatatc gctgcctgct actcagaccc agcaaaggct aaagcagaac tcggttggga
     3961 agcagaactc gacatcaccc aaatgtgtga agacgcatgg cgttggcaaa gcaagcatcc
     4021 aaatggattt gaagactaag atgatgattt caatcatcgt cccttgttta aacgaagagg
     4081 aagtacttcc tcttttttat caggctctgg aagctttact tccagatttg gaaacagaaa
     4141 tcgagtatgt ctttgtcgat gatggatcaa gtgatgggac cttggaactc ttaaaggcct
     4201 atcgggagca aaatccggca gtccattata tttctttctc tcgaaatttt ggcaaagaag
     4261 cagcccttta tgcaggcttg caatatgcga caggagattt ggtggtggtg atggatgcag
     4321 acctccaaga tcctcctagt atgttgtttg agatgaaaaa tgtactagac aaaaatgtag
     4381 acttggactg cgttgggaca cggagaacta gtcgggaggg agaacccttc tttcgcagtt
     4441 tctgtgctgt tctcttttat cgcctcatgc aaaaaatcag cccagtagct ctgccgtcgg
     4501 gtgtccgtga ttttcgtatg atgagaaggt ctgtagtcga tgccatttta agcttgaccg
     4561 agtccaatcg tttttcaaag gggctcttcg cctgggtggg tttcaaaacc tactatttgg
     4621 actatccaaa tgtcgaaagg caggctggca agacaagttg gagttttagg caactctttt
     4681 tctactccat tgaagggatt attaattttt cagattttcc cttgattata gcctttgtag
     4741 ctggtctcct atcttgtttt ctttctctgc tgatgacctt ttttgttgtg gttcggaccc
     4801 tcattttggg caatccgaca tctggttgga cctctctgat ggctgttatt ctctttcttg
     4861 gaggcattca actcttgacc attgggattc tcggcaagta tatcagtaag atttatttag
     4921 agactaaaaa aagaccactt tatcttatca aagaaaaaag tgaccttcct gattttacag
     4981 aaaaaaataa agtgaaaaga ctataatttt acatggaaat gtgctaaact agagggagtg
     5041 gattatcgcc attgatttag gcttcctggt ttccaatcag ggcgcgagtt gggcaatttt
     5101 taacagcctc taagacatcc tgactaggag aaatttcttt ttccagttgg tcagggtcat
     5161 cgtaaaaacg cacgattcca ttatcgtggt aatcaaataa atcagaataa gtttggcaaa
     5221 gcccacaggc gatacatcgt tcaggtataa gtgtgatttt catgttcata tttatattgt
     5281 aataagaaaa gtaaaaaaaa acaaggagta aggtatggca aaagaaccgt ggcaagaaga
     5341 tatctatgat caagaagaat caagagcaga gcgtcggcat cgaaaccacg gaggggctga
     5401 taggatggct aatcgtattt tgacgatcct agctagtatt ttctttgtaa ttgtggtggt
     5461 gatggtcatc gttctcatct atctatcatc ggggggaagt aatcgcacag cagccttaaa
     5521 agactttcat gattctgatg caagtgtagt acaaatctca tcttcaagca gttctcagcc
     5581 tgagcagagt tcagagccag aatctacttc tagtagttca gaagaagctg ctaatcctga
     5641 aggaacgatt aaagttctcg caggagaagg ggaagcagct attgccgctc gtgcaggaat
     5701 ctccattgct cagttagagg ccttgaatcc tgggcacatg gctacaggat cttggtttgc
     5761 taatccaggt gatgttataa aaataaaata ggagccgata tgaaaacaat tcaaattgct
     5821 attgatggtc ctgcttccag cggtaagagt acggtcgcaa agattattgc taaggatttt
     5881 ggattcacct accttgatac aggagctatg tatcgtgcag cgacctatat ggctcttaag
     5941 aaccaattag gagttgaaga agtcgaagcc cttctagcct tgttggacca gcatccaatc
     6001 agctttggac gttcagaaac tggagaccag cttgtttttg taggagatgt ggatattacc
     6061 catcctatcc gtgaaaatga agtgaccaat catgtttctg ctattgcagc aattcctgaa
     6121 gtgcgtgaga aactggtttc tctccaacaa gaaattgccc agcaaggcgg gattgtcatg
     6181 gatggtcgcg atattggaac tgttgtattg ccacaagcag aattgaaaat tttcctagta
     6241 gcttctgttg atgagagagc agagcgtcgt tacaaggaaa atattgccaa gggaattgaa
     6301 acagaccttg aaaccctaaa aaaggaaatt gctgcgcgtg actacaagga tagtcatcgt
     6361 gagacttctc ctctcaaaca agcagaggat gctgtctacc ttgatacaac tggtttgaac
     6421 attcaagaag tagttgaaaa aatcaaagca gaagctgaaa aaagaatgta aatgtagaaa
     6481 agccgataga ttgatatcgg tttttttcta aatttgtcaa aatgctgatt ttagggtata
     6541 atagttgctg ttcgagaaat tttgattttc aaagaatagt gaatgatgat aaggagaaac
     6601 catgaacaat ttaccaaatt gtccaaaatg taactcagag tatgtctacg aagacggtgc
     6661 tctactggtt tgcccagagt gtgctcatga gtggaatcct gctgaagttg cagaagtaga
     6721 agaaggtctt gtcgctatcg atgccaacgg aaataaattg actgatggtg atacagtaac
     6781 tctcatcaag gacttgaaag taaaaggtgc gccaaaagat ttgaaacaag ggacgcgcgt
     6841 gaaaaatatc cgcatcgtag aaggcgatca caatatcgac tgtaagattg atggttttgg
     6901 tgccatgaaa cttaaatcag agtttgtgag gaagatttaa gcatgtcaaa acactataaa
     6961 cttgtatttt atagccgtat cttcttgttt ctagcggctt ttacgggagt ttatcttgaa
     7021 attactaggc atggtggttt tgggatgctt ctctattaca cggttctgtc caacctcttg
     7081 gtaactattt ttacccttta tcttctaaag gttatgagcc gtgtaggtga aaattggcaa
     7141 aggccaagtc tcttgcgctt aaaaggtggg gtcaccatga gtatcatgat tacctgtgtg
     7201 atttaccatt tcctcttggc gcccattgcg actaatttct ataccctaga aaatttcctt
     7261 tgccactata tcgttcccat ctggttttta gcggataccc tcttttttga caaacagggt
     7321 caatacaaga tttgggatcc agcagtgtgg accattttac cctttctgta tatgatgttt
     7381 gctcttttta atggcttggt tctaaaactc aatattccaa atgccaagga taatcctttc
     7441 ccttacttct ttttgaatgt gaacaagggt tggaatgttg tgtttaagtg gtgtctgatt
     7501 atctttgttg cctatatggt agcaggattt attttctact ttatcaagca aatcaagaga
     7561 aagtcatcct aatactcttc gaaaatctct tcaaaccacg tcagtttcca tctgcaacct
     7621 caaaacactg ttttgaacaa cctgcggcta gcttcctagt ttgctctttg attttcattg
     7681 agtataagat acaagggatt cattttcgaa tttaagaagg agtctacaaa ccaatgaggc
     7741 ttcttctttt cttgcttatt tgataactat caaaaaagta gaagattaag agaaatatag
     7801 aaagagattt catgaaacaa tttttagaac gggccagcat tttggctctc tccctcgttt
     7861 tgattacctc cttttccatt tcgagtgccc taccagccat gtttgactat tatcagggtt
     7921 attctaagga acaaattgag ctcttggtga gcttgccttc ctttggaatc atgatgatgt
     7981 tactgctaaa tggtttctta gaaaaaatat ttcctgagcg cttacagatt agtttgggct
     8041 tgctgatttt atcattgagc ggtacagctc ccttctggta ccaagcctat ccctttgtct
     8101 ttggaacacg gcttctcttt ggtttgggtc ttgggatgat caatgccaag gccatttcta
     8161 ttatcagtga acgctaccaa ggaaaaaggc gaattcagat gttagggcta cgcgcttctg
     8221 cagaggtcgt tggagcttct ctcattacct tggccgtcgg tcagttgttg gcctttggtt
     8281 ggacagctat ctttctagcc tatagtgctg gatttttggt gctgcccctt tatctgctct
     8341 ttgtccctta tggaaaatca aagaaagaag tcaagaaaag agcggaggaa gcaagtcgtt
     8401 taactcgaga aatgaaaggc ttgattttta ccttagctat cgaagcggca gttgtagttt
     8461 gtaccaatac agctattacc atccgtattc caagtttgat ggtggaaaga ggattggggg
     8521 atgcccagtt atctagtttt gttcttagta tcatgcagtt gatcgggatt gtggctgggg
     8581 tgagtttttc tttcttgatt tctatcttta aagagaaact gctcctctgg tctggtatta
     8641 cctttggctt ggggcaaatt gtgattgcct tgtcttcatc cttgtgggtg gtagtagcag
     8701 gaagtgttct ggctggattt gcctatagtg tagtcttgac gacggtcttt caacttgtct
     8761 ctgaacgaat tccagctaaa ctcctcaatc aagcaacttc atttgctgta ttaggctgta
     8821 gtttcggagc ctttacgacc ccatttgttc taggtgcaat tggcttacta actcacaatg
     8881 ggatgttggt ctttagtatc ttaggaggtt ggttgattgt aatctctatc tttgtcatgt
     8941 acctacttca gaagagagct taggattgat tcctaagttt ttcttttgat actcaatgaa
     9001 aatcaaagag catagttgat tgagtttgga atagtacgct gtagttgcta aaatat
//
LOCUS       DAVMEF010000072         8936 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMEF010000072 DAVMEF010000000
VERSION     DAVMEF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.072"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            111..3212
                     /locus_tag="VYQ30_001238"
     CDS             111..3212
                     /locus_tag="VYQ30_001238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1966346.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRANLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLV
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRSMGKKDASAMHEMRASFIQGSIEAGHTAEKAGQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKHPLLEPLVKV
                     GLFDLFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVRKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3294..4301
                     /gene="pfkA"
                     /locus_tag="VYQ30_001239"
     CDS             3294..4301
                     /gene="pfkA"
                     /locus_tag="VYQ30_001239"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1966347.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSADEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4360..5865
                     /gene="pyk"
                     /locus_tag="VYQ30_001240"
     CDS             4360..5865
                     /gene="pyk"
                     /locus_tag="VYQ30_001240"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1966348.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6185..6433)
                     /locus_tag="VYQ30_001241"
     CDS             complement(6185..6433)
                     /locus_tag="VYQ30_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1966349.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(6600..6825)
                     /locus_tag="VYQ30_001242"
                     /pseudo
     CDS             complement(6600..6825)
                     /locus_tag="VYQ30_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7673..8545
                     /locus_tag="VYQ30_001243"
     CDS             7673..8545
                     /locus_tag="VYQ30_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1966350.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFNSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8691..8936)
                     /locus_tag="VYQ30_001244"
                     /pseudo
     CDS             complement(8691..8936)
                     /locus_tag="VYQ30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2725 a         1564 c         1921 g         2726 t
ORIGIN      
        1 tacaaatgaa tagaaaataa attagtaagc tgatgaaatt tttctcaaga gaagctattt
       61 ataggtgaaa atggtataat atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac
      121 aactagatac aaaaacagtc tatagtttta tggaaagcgt catttcgatc gaaaagtatg
      181 tgagagcagc taaagaatac ggctacactc atttggctat gatggatatt gacaatcttt
      241 atggcgcttt cgactttcta gagattacaa aaaaatacgg cattcatcct ttgctagggc
      301 ttgaaatgac agtgtttgta gatgatcagg aagtgaattt gcgcttttta gctctatcta
      361 gtgtgggcta tcagcagttg atgaagcttt cgacagccaa gatgcagggg gagaaaactt
      421 ggtcagtcct gtcccagtac ctggaggata tcgcggtcat tgtgccttat tttgatagag
      481 ttgagtcgtt agaactaggc tgtgattact atataggggt ttatccagaa acactagcaa
      541 gcgaatttca tcatcctatc ttacctcttt atcgggtcaa cgcttttgaa agcagggata
      601 gagaagttct tcaagtttta acagcgatta aagaaaatct accgctcaga gaagttccct
      661 tgcgttcgag acaagatgtc tttatatcag caagttcttt agagaaacta ttccaagagc
      721 gttttccgca agctttggac aatttagaaa agcttatttc aggcatttct tacgacttgg
      781 atactagtct gaaactgcct cgttttaatc cagctagacc agcagtagag gagttgagag
      841 agcgtgctga actggggctt gttcagaagg ggttgactag taaagaatat caagatagac
      901 tagaccaaga attgtctgtt attcatgata tgggctttga tgattatttc ttggttgttt
      961 gggatttgtt gcgttttgga cgatcgaatg gctattatat gggaatggga aggggttctg
     1021 cagtaggcag tttggtttct tatgccttag acatcacggg gattgaccca gtagagaaaa
     1081 atctgatttt tgaacgtttc ctcaatcgtg aacgctacac aatgccagat attgacattg
     1141 atatcccaga tatttatcgt ccagatttta tcagatatgt tggcaataaa tatggtagta
     1201 aacatgcggc acaaatcgtt actttttcaa cctttggagc caagcaagct ctacgagatg
     1261 tcttgaaacg ctttggtgtt cctgagtatg aattatctgc aattactaag aaaatcagtt
     1321 ttcgtgccaa tcttaagtcg gcctatgagg gaaatctcca gtttcgtcag caaatcaata
     1381 gtaagttaga ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc tatccaaggc
     1441 aaacctctgt ccatgcggct ggtgttgtaa ttagtgacca agatttgacc aactacattc
     1501 ctctaaagta tggtgatgaa attccactga ctcagtatga tgctcatgga gttgaggcta
     1561 gcggactttt gaagatggac tttctgggac tacgaaattt gacctttgtc cagaagatgc
     1621 aagagttgct tgttgaaaca gaaggtattc accttaaaat tgaagaaata gatttggaag
     1681 acaaagaaac gttagattta tttgcctctg gaaatacaaa aggtatcttt caatttgagc
     1741 aacctggtgc tattcgcttg ctcaaacgtg ttcaaccagt ctgttttgaa gatgtcgtag
     1801 caactacttc tctaaatcga ccaggtgcaa gtgactacat caataatttt gtggcaagga
     1861 agcatgggca ggaagaagtg actgttctag attcagctct ggaggatatt ttggctccaa
     1921 cttatggcat tatgctctat caggagcagg ttatgcaggt tgctcagcga tttgctggat
     1981 ttagtcttgg gaaggccgat attttgcgtc gatctatggg taaaaaggat gcctctgcta
     2041 tgcatgagat gagggcttcc tttattcaag gctccataga agcaggccat actgcggaaa
     2101 aagcagggca ggtttttgat gttatggaga agtttgcagg ctatggtttt aacaggtcac
     2161 acgcctatgc ctactcagca ttggccttcc agttggctta ttttaaaaca cattatccag
     2221 ccatatttta tcagattatg ttgaattctg ccaacagtga ttatgtaaca gatgccttag
     2281 aagcaggttt tgaagtagcc ccattgtcca tcaatactat tccctatcac gataaaattg
     2341 ccaacaagtc tatctatata ggtttgaaat cgattaaggg gctcagcaag gacttggcgc
     2401 tttggattat tgaacataga ccttattcta atattgaaga ttttatagct aaattacctg
     2461 agaattatct gaaacatcct ctgctagaac ctttggtaaa agttggcctt tttgatttat
     2521 ttgaaaaaaa tcgtcaaaaa gtatttaata acttagctaa tctatttgaa tttgtgaaag
     2581 agttgggaag tttgtttgga gatgctattt atagttggca ggaatcggaa gattggacgg
     2641 aacaagaaaa attttatatg gaacaagagc ttttagggat aggtgtcagc aaacatccac
     2701 tacaagctat tgcaagtaag gctatttacc cgattacccc aatcggaaat ttgtcagaaa
     2761 atagctatgc tattattttg gttgaagttc ggaaaataaa agtgattcgt accaaaaagg
     2821 gtgaaaatat ggccttctta caggcagatg atagtaagaa aaaattggat gtcactctct
     2881 tttcagactt atatcgtcag gttggacagg aaataaaaga gggagccttc tactatgtaa
     2941 aaggaaaaat acaatcacgt gatggccgtc tgcaaatgat tgcacaagaa ataagagaag
     3001 cagttgctga acgcttttgg atacaggtga aaaatcatga atcggatcaa gaaatttcac
     3061 gtattttaga acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga
     3121 aaaccatcgt ttctccccat cattttgtag ctaaatccaa tgaattagag gagaaattga
     3181 atgaaatcgt tatgaaaacg atttatcgct aaaaatacgg aaaatagaag aattttaaaa
     3241 tcaaatgtga tataatcaag aagaatgtta aaagaaaaag gagcataacc aatatgaaac
     3301 gtattgctgt tttgactagt ggtggagacg cccctggtat gaacgctgcc atccgtgcag
     3361 ttgttcgtca agcaatttca gaaggaatgg aagtttttgg tatctatgac ggatatgctg
     3421 gtatggttgc cggtgaaatt catcccctag atgcagcttc agtaggagac atcatttctc
     3481 gtggtggtac ttttcttcac tcagctcgtt atccagagtt cgctcaactt gaagggcaac
     3541 ttaaagggat tgagcaattg aaaaaacacg gaattgaagg tgtagttgtt atcggtggtg
     3601 acggatctta ccacggcgct atgcgtttga ctgaacatgg cttcccagct attggtcttc
     3661 caggtacaat cgataacgat atcgttggta ctgactttac aatcggtttt gacacagcgg
     3721 ttactactgc catggacgct atcgataaga ttcgtgatac atcatcaagt caccgtcgta
     3781 cttttgtaat cgaagttatg ggacgtaacg ctggtgatat cgctctttgg gctggtattg
     3841 caactggtgc tgatgaaatc atcatccctg aagcaggctt caagatggaa gatatcgtag
     3901 caagcatcaa agctggttat gaatgtggta aaaaacacaa tatcatcgtc ttagctgaag
     3961 gtgtgatgtc agcggatgaa tttggtcaaa aacttaaaga agctggagat acaagcgacc
     4021 ttcgtgtaac agaacttgga catattcaac gtggtggttc tccaactgcg cgtgaccgtg
     4081 ttttggcgtc acgtatgggt gcacatgctg ttaaacttct taaagaaggt atcggtggtg
     4141 ttgcggttgg tattcgtaac gaaaaaatgg ttgaaaatcc aattcttggt actgcagaag
     4201 aaggggcatt gtttagcctt actgcagaag gtaagattgt ggttaacaac ccacacaaag
     4261 ccgatattga gctatctagc ttgaataaga gcttgtcata atttacaatt tagttaataa
     4321 gaccaaaaag gtcatatata taaaggagtc acaaaaatca tgaacaaacg tgtaaaaatc
     4381 gttgcaactt tgggtcctgc ggtagaaatc cgtggtggta aaaaattcgg tgaggacgga
     4441 tactggggtg aaaaacttga tgttgaagct tcagctaaaa acattgctaa attgattgaa
     4501 gctggtgcta acacattccg attcaacttc tcacacggcg accaccaaga acaaggtgag
     4561 cgtatggcaa ctgttaaact tgcggaaaaa attgcaggta aaaaagttgg tttccttctt
     4621 gatacaaaag gacctgaaat ccgtacagaa ttgttcgaag gtgaagctaa agaatattca
     4681 tacaaaactg gtgaaaaaat tcgtgttgca actaaacaag gaatcaaatc aactcgtgaa
     4741 gtgattgcgt tgaacgttgc tggtgctctt gatatctatg atgatgttga agttggtcgt
     4801 caagttttgg ttgacgatgg taaacttggt cttcgtgtgg ttgctaaaga tgatgcaact
     4861 cgtgaatttg aagttgaagt tgaaaacgat ggtatcatcg ctaaacaaaa aggtgtgaac
     4921 atccctaaca ctaaaattcc tttcccagct cttgctgaac gcgataacga cgatatccgt
     4981 ttcggtcttg aacaaggtat caacttcatc gcaatttcat tcgtacgtac tgcaaaagat
     5041 gtgaacgaag ttcgtgcaat ctgtgaagaa actggaaacg gacatgttca attgttcgct
     5101 aaaatcgaaa accaacaagg tatcgataac ttagacgaaa tcatcgaagc agctgatggt
     5161 attatgattg ctcgtggtga tatgggtatc gaagtaccgt tcgaaatggt tccagtttat
     5221 caaaaaatga ttatcaagaa agtcaatgct gcaggtaaag ttgttatcac tgcaacaaac
     5281 atgcttgaaa caatgactga aaaaccacgt gcaactcgtt cagaagtatc agacgtattc
     5341 aacgctgtta tcgacggaac tgacgctaca atgctgtcag gcgagtctgc aaacggtaaa
     5401 tacccactcg agtcagtaac tacaatggct acaatcgaca agaacgctca agctcttctt
     5461 aatgaatacg gacgtcttga ttcagattca tttgagcgta actctaagac agaagtaatg
     5521 gcttctgctg ttaaagatgc tactagctca atggatatca aattggttgt aactcttact
     5581 aagacaggtc atactgcacg tttgatttct aaataccgtc caaatgctga catcttagca
     5641 ttgacatttg acgaattgac agaacgtggc ttgatgttga actggggtgt tatcccaatg
     5701 ttgacagatg ctccatcttc aactgacgat atgttcgaaa tcgctgaacg taaagcggta
     5761 gaagcaggtc tcgttgagtc aggcgatgat atcgttatcg ttgctggtgt gccagtagga
     5821 gaagctgttc gcacaaacac aatgcgtatc cgcacagtac gttaagaaaa atataaaaac
     5881 ctatcatatc cagctttcta gcctgtatga tgggctcttt ttttgtccag ggggcaaaaa
     5941 gggggcaaaa ctttcataat ggattgatac tcttcgaaaa tctcttcaaa ccacgtcagc
     6001 gtcgccttac cgtatatatg ttactgactt cgtcagttct atctacaacc tcaaagcagt
     6061 gctttgagca acctgcggct agcttcctag tttgctcttt gattttcatt gagtatgaaa
     6121 taagatatgc acaaattgat tagaaagtca aatgaatttc tacaaatgtt ttagcaatcg
     6181 caatctactt gtctagattc gatctgatat attttcgatt taatgatatg gtatttaaaa
     6241 cctccaaagt agcttgctcc attcttttac ttacgtgagt gtagatgtta tttactgttt
     6301 tagcgttttt gtgttccact ctaaccatta tagcattctt ctcagctagt gtactaagga
     6361 gtgtgtgcct gaaaatatgg gaactaaggg gctggtttat cggtttctct agtttagtat
     6421 ttgccttttg caaagtgatc ttaaatgcct ttctctaaat ttacatatca ctattgttta
     6481 acaaaatcta atctacttta ggtcacttat tctttttttg aaatgtagaa tgaacttttt
     6541 caaagttttt cgaatctttt aaaatctgtt tgttttatat cgccattctc cccccttttt
     6601 taattctccc tatatagcct gacaactttc ccgatggtac gaatatggtt gctttcgtct
     6661 aggtggatgt cggggtattc gggattgagt ttttttgagg tagccttggc ggagtttctt
     6721 gacatagtta gcgccgtcta cttggaagat gccgatggta ttatagtcaa tctgtggggt
     6781 attcttgata aataggtagt cgctgtttct tatctttggc tccatggact tgctgacgac
     6841 ataagcgatt gggtcgtagt cgtctgggat aatggaaact ccataactaa atcgtcgtcc
     6901 tgcatcgagc ggctacctgc agagataaac tacctaacac gagagtaagt agtctgtctg
     6961 tagtcgtcca gtctgatgat ttttacgata cttcgttttt ctgatcatac agttgcctct
     7021 cggcataggt cagaacttta ccttgtctgg gtggtgcccg ttggtcgtag atagattgga
     7081 tatcgctagg agaatccttt tgaactggag gaaagagggc atcaatcaag ctactgaata
     7141 ctttaactaa gtcaaatata gtatttttct tagtagacct aacccttttt tcataatttc
     7201 taatggtgtt tttacttata cctatcttag tacccaattc ttattgagtc caaccattac
     7261 tagtctatat tgttttatag ttgatttagt ttggaatagt acgctgtagc tgctaaaaca
     7321 tttctaggaa ttaatttgcc tttcctaata gagttgttca tatcttattt caatctacta
     7381 tatttttcac ctctaacaat cgcaatctct tctttatcca tgaataaaaa cgatttctat
     7441 ttttgtaagt aaagcatcac acgaaatcca cgaaaatgaa aacctttgtt gtgttttcgt
     7501 aaaaaatttg ttgacagagc acgaaacgcc tactataatt aaatcaagct taaggaaata
     7561 acaaaaaaac aaatcggagg gaaagacgat gaacacatta aatgagaaag taatcaatat
     7621 ctgtaaagca gtagttaaag aaactttaat ccaagacatt taggagaaag aaatgaagaa
     7681 atcaagaaaa ttagccacat taggaatctg ttcagccttg tttttaggct tggctgcttg
     7741 tcaacaacaa catgctactt ctgaggggac gaatcaaagg caaagcagtt cagcgaaagt
     7801 tccatggaaa gcttcataca ccaacctaaa caaccaggta agtacagaag aggtcaaatc
     7861 tctcttatca gctcacttgg atccaaatag tgttgatgca ttttttaatc tcgttaatga
     7921 ctataatacc attgtcggct caactggctt atcaggagat ttcacttcct ttactcacac
     7981 cgaatacgat gttgagaaaa tcagtcatct ctggaatcaa aagaagggcg attttgttgg
     8041 gaccaactgc cgtatcaata gttattgtct tttgaaaaat tcagtcacca ttccaaagct
     8101 tgaaaagaat gaccaattgc ttttcctaga taatgatgcg attgataaag gaaaggtctt
     8161 taattcacaa gataaggaag agtttgatat tctattttcg agagttccaa ctgaggcaac
     8221 tacagatgtc aaggttcacg ctgaaaagat ggaaacattc ttctcacaat ttcaattcaa
     8281 tgaaaaagct cgaatgctgt ctgtagtctt gcacgacaat ttggatggcg agtatctgtt
     8341 tgtaggccac gttggggtct tagtacctgc tgatgacggt ttcttatttg tagagaaatt
     8401 gactttcgaa gagccctacc aagcgattaa atttgctagt aaggaagatt gctacaagta
     8461 tttgggcacc aagtatgcgg attatacagg cgagggactg gctaagcctt ttatcatgga
     8521 taatgataag tgggttaaac tttaatagtg ttatgatatg acagtaatct taggattttt
     8581 atttctattt ttgatgagtt ggtgggttat ttcaatcgtt aatagccaga aaccactcaa
     8641 aaatgaaaca tttattggct atgtccaaag atacgatatt gatgggaatg gctatcaatc
     8701 attaccgtgt cctcagaact gagaggagtt cttgaaatcg taaaaccact ttgaacaaga
     8761 gttacttcaa cccattggct ctgatggatt aagttgcttt cgtgaatacc aaaatcagcc
     8821 gcaatttctt cataagtgcg gtattctcgc acatattgaa gagtggccat aagaaggtct
     8881 tctaggctta atttaggttt tcgtccacct tttgcgtgtt taagttgata agctgt
//
LOCUS       DAVMEF010000073         8750 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMEF010000073 DAVMEF010000000
VERSION     DAVMEF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.073"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(77..967)
                     /locus_tag="VYQ30_001245"
     CDS             complement(77..967)
                     /locus_tag="VYQ30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1966351.1"
                     /translation="MKAKYAAWVAFFLNLTYTIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            1100..1636
                     /locus_tag="VYQ30_001246"
     CDS             1100..1636
                     /locus_tag="VYQ30_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1966352.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISNEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(1802..2608)
                     /locus_tag="VYQ30_001247"
     CDS             complement(1802..2608)
                     /locus_tag="VYQ30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1966353.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(2953..4473)
                     /locus_tag="VYQ30_001248"
     CDS             complement(2953..4473)
                     /locus_tag="VYQ30_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1966354.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(4466..5194)
                     /locus_tag="VYQ30_001249"
     CDS             complement(4466..5194)
                     /locus_tag="VYQ30_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966355.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(5209..5973)
                     /locus_tag="VYQ30_001250"
     CDS             complement(5209..5973)
                     /locus_tag="VYQ30_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1966356.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            complement(5977..6420)
                     /locus_tag="VYQ30_001251"
     CDS             complement(5977..6420)
                     /locus_tag="VYQ30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1966357.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(7102..7398)
                     /locus_tag="VYQ30_001252"
     CDS             complement(7102..7398)
                     /locus_tag="VYQ30_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1966358.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(7420..8484)
                     /gene="pepA"
                     /locus_tag="VYQ30_001253"
     CDS             complement(7420..8484)
                     /gene="pepA"
                     /locus_tag="VYQ30_001253"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1966359.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGNGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         2615 a         2064 c         1493 g         2578 t
ORIGIN      
        1 atttttgaaa tatttaaata ataaagaaat tactctattc caaataagat agtacccaag
       61 tatttttcac ttttttctaa tgttgatgct cataactccg ttccaagtca cacacctttc
      121 gcttatgggt ttggtgagtt tctaggtcag catcaatttc aatggtaata ttttgaaaac
      181 cacaatcttt taggaaaatt cgaatagact ctttacaagt ttccatatgt tccatttctt
      241 ttagacaaac atggacaatg gcattttttt ccaaagcatc catagtccag agattaagct
      301 gattaaggct gaccacattg tccaatcgct ccaggccact ctttacttgc ttgatatcaa
      361 gaccttctgg cacagcatcc aagaaaatct tgagtgtaga ccaaaaacgt ggaagggctt
      421 ttgaaagaat aaagaaagaa atgacaaggg acaaaagagg atctaggata taccagtccg
      481 taaatcgaag aacaatcgcc atcaggataa cagctaccca ccctagcgta tcttccagaa
      541 aatgcagact cagaatagac tcattctttg tctttccctt accaaccacc agactcgcta
      601 acagattgat agtaatcgca ataattccta accagagaat cccctcatca ttgactggtt
      661 gcggatgcaa aatcttcgtg acattttcca aaatgactag aacagagcct gttacgagaa
      721 tcacagctgt taccaaggct cctagcaggc taaaccgctt atagcccaag gtgtactgat
      781 tgtcttcttc acgattggag attgtttcta gaaaagctga tattccaatt gcaatcacat
      841 ctcccaagtc atgcacagag tcagcaagaa cagcgctaga accaaatact ccacctgcaa
      901 taaactcaac aatggtataa gtcaaattta agaaaaaagc cacccaagca gcatattttg
      961 ccttcatatt tctcattcct ttgttataat agatttatga acaccttgtt cattatcatt
     1021 atccactaaa actcaaagaa aggatagaag caaaacgtca gctttattca gttctgaaca
     1081 atttgcctca agtgtccata tgactaacat tgaccgccgt atcagcaaaa ccaaaaaagc
     1141 catctatcaa gcttttatac aacttttgaa tgctaagggc tacgaggcca ctactgttca
     1201 ggatatcatt gatctcgcag atgtgggacg atccaccttt tactgtcact atgagagtaa
     1261 ggagcttctt ctggaccagc tctgccgcta cctcttccat catctttttg aaagagagca
     1321 agccatttca aacgaggact acctcgccca cctctttctg cattttcata aaaaccaaga
     1381 ccatatcacc agtctgctat tttccaaaaa tgactacttc ctccgtcaac tccataaaga
     1441 gctagaacac catgtctatt ccgtgctagc tgataattta aaagaagccc accctaatct
     1501 gcctacttct tacctccaac acttggtcat gtccaacttt atcgagacat tgacctggtg
     1561 gctcaaaaaa ggtcaagatt tcacagacca ggaagttgtc caattttatc tagaccttct
     1621 cattcctaaa aattgaatat agagtaaagc tcagttgtct tatttctagg ttactgagtt
     1681 ttttatcttt tcaacaacaa aagaggaccc gccgatcctc tttttcatac tataaatcct
     1741 tgattatcaa ctatatctgt tttaatcgaa atctcaaaac agcactttca aacatctttt
     1801 cctagttaag taaatcagta ttctgcttag ctgccttgct ccattgatac caaccaacta
     1861 gactgttaat gagataaatt agatatttcc cttgaatttg caggctttct ccccaccaga
     1921 gatagattga aaaaacattg gtagccgccc agaatatcca ctgttcacgg taaacagctg
     1981 tcatgaggat ttgccctacc ccattggttg catctgtgat tgaatcacga tagggacgat
     2041 tggcaccaat agactgataa atgaagccaa aggccaacca ccaaagcaca ctaatggaaa
     2101 gatactttgt ccagcccttg ccgtccagtt tacgcgcgac aaactcctgc ttttccttct
     2161 taaactgtgc ctgataaatc caaactagaa gtccaattgg ctgcatgact gtgaagtaaa
     2221 gtgtcgtcag cacctcacca taaaagcctt tctgtagggc caaaataagg taaataacag
     2281 agttaatcaa gccaaaaaga taattacttg ctcgaccttc cgatacaaag attacacaga
     2341 taatccctgt caagctacaa atcatcccaa tccagtcaac aatacgatgt tcgtaaacca
     2401 actccagcca gagaggaaaa cttcctaaaa ccagcaaata aatccactgg gcaaaactac
     2461 gatgggcaaa gaggtcatcc cagatagcct tcatagttcc tgaaaatcct aaatcagcca
     2521 tagccgcaac catacgacgg taaccacctg acatttcacc tagggttgtt ttgatatttt
     2581 caattttctt ttgcaaataa gtatgcatca tttctccttt tgtttttaaa gagccgtgtc
     2641 tggatagact ttcggacgca acgctctatt agataatgaa ctgcctatac acaagatttc
     2701 taaccttagt cgacatgagc tgaaacctct tatttgttaa gtagttcaca aaatattata
     2761 cacctatttt atgaatagtc aactgtcttt acagtaaaat tttagaaaat catgaaaatt
     2821 ttctctttct ttccatttta agtgacattc agtcattctc acatcaaaaa agcccagacg
     2881 aaattgtctg agcattcttt tatctagtcg tttaaggaag ttgagttcag tatgtttaaa
     2941 gtctctgtcc catcatttct tcaacaaacc ttgttcttgg agaaactcct tggctacttg
     3001 ctttgctgac ttgccttcaa caccgacttg gtagttgagc tggctcatct ggctttctgt
     3061 aatcttacca gccaatgtat taagaactct ttccaactct gggtgtttct tgagaagagc
     3121 ttctttcatg agtggagccc cttgataagg tgggaagagt tgcttgtcat cttccaagac
     3181 ctgtaaatca taacgctcca attccgcatc agtcgaatag gcatccgtga tttgaatatc
     3241 ccctgactga attgcctgat aacgaagagc tggctgcata gtcgccacat tgagattgag
     3301 accatacatt gattgcaagc ccttatttcc atcttcacgg tcgttaaact caagcgtaaa
     3361 gcctgccttc agctgccctt ccactttttt caaatccgaa atggtcttca agccatattc
     3421 ttgagcaatc tttttcggaa cagctacagc ataggtattt tgataagaca taggtttgag
     3481 ataggctaga tgatcctgct tggcaatgcc atcacgcgcc acttgataaa cctgctctgg
     3541 ctcatgactc accttgggtg atggttgaag caaactttca gtcaccgtac cggtaaattc
     3601 aggataaatg tcaatatcac cttttttcag agcttcataa aggaaacttg tcgtcccaaa
     3661 attcggttta acagtcgcag tcatgctggt attttcttca atcagcaatt tatacatatt
     3721 agccaaaatt tctggttctg ggcctatttt cccagcaata atcaagtttt ccttctcttt
     3781 ttgagccaaa agggctggac tataagacag acccagtaat aaagccacca aggcaaaacc
     3841 agagaaaatc gtccgcaatt tcgctttttc cattactttt agtaggaagt taaaggcaat
     3901 agctagcact gcagaagaaa gggccccaat caaaatcaaa ctggcattat tacggtcaat
     3961 tcccaacagg ataaaggaac ccagtccccc tgccccaatc aaggtcgcca aggtagccgt
     4021 accgataatc aagaccgctg ccgtccgaat tccagacatg ataacaggca tggcaagtgg
     4081 aatttcaaac ttcttgagac gctcccatct ggtcatccca aaggcaatcc cagcctcttg
     4141 aagactcgga tcaattccct tcagcccagt aatagtattt tgtaaaatcg gaaaaatcgc
     4201 ataaatcact agagctgtca atgccggtaa agtcccaatt cccatcaagg gaataaagag
     4261 tcccaacaag gccagagacg ggatggtctg gaaaatccct gcaatctgta agacccaatc
     4321 cgccaacttc tcatgatagc gaagataaac agccaaggga atcgcaagca aaatagctag
     4381 taacaaggtc aaaagtgaca actgcaaatg ttgagatagg gctgtcaacc aatcaccaaa
     4441 acgatcctga aaagttgcaa ttaaattagt catgaatact acctccaaac aagtctgcaa
     4501 caaagtctgt tgcaggagct tttaaaatag tctcaggatt cgacacctga cgaatctctc
     4561 catcctgcaa aacagcaata cgatctgcca actttaaggc ttcatccgta tcatgagtca
     4621 caaaaatcgt tgtcatccca aactctttat gtaattcttt tgtcagaacc tgcaactgct
     4681 tgcgagaaat agcatccaag gctgaaaagg gttcatccat gaggagaatc ttgggctgac
     4741 caatcatagc tcggacaata ccgacccgtt gctgttctcc accagatagc tcacttggaa
     4801 gtcgatgccc atactcagct actggtaatc caacttttgc caaaagctct tctgtttttt
     4861 gagcaatttc ttccttagtc caacccttca tttcaggaat gagagcaata ttttcctcaa
     4921 ctgttagatt gggaaagaga gcaatagcct gtaaaacata tccagtagta agacgaagtt
     4981 cacgctcatc atagtctttg atgcgcttgc catccatata aatatttcca tcagttggtt
     5041 ccaagagacg gttaatcatc ttaatcatgg tcgtcttacc tgacccagaa ggcccaacta
     5101 aaaccatgaa ctccccattc tcaatctgta aattgacatc tctcaagaca tctttttctg
     5161 tgtagcgcag tgctacattt ttgtattcaa tcattctttg tcctcaattt aaaacttccc
     5221 tcgattagtc aagtcttcta ctttaggtat gacttcctta ttatcccaat gctccacaat
     5281 tttcccgttc tctaaacgga agatatcgta ctgggcataa gcaacgccat caatctgagt
     5341 ctgaccatag ctaaccacat agtttccttg tcctaagagt tggaaaacaa agtcaaaagt
     5401 gacactatat tcagccacat agtttttata agcagcactt ccttgtccaa tctcatgatt
     5461 atgctgaatc aaatcgtctg ccacataatc actccactgc tctagctccc cattttggaa
     5521 agtttctgtc aagaaacggc gaaccagctt tttattttct gctttcttgt ccaaatcctt
     5581 gatttcaaaa tctccaaaaa tttgatctag ttggtcattt tcaggtgttc gatagtagtc
     5641 aatgacatcc caatgctcaa caatacgacc attctcatcc gcacggaaag tatccgtcgt
     5701 cacccattga gcttctccac cattcagata ttgatgaaca tgaacaaaga ccagattgcc
     5761 atcctcaatg gtgcggacaa tcttaatctg acgctctgga tgacgctcaa agaaatctgc
     5821 aaagaaggct gcaaatcctt ctttcccgtc aggaacacct gtcgaatgtt ggatataggt
     5881 atcccctaca gactgggctt gagcctcagc aactcgtccg tcttgaatgg catggatgta
     5941 taggttgtga gcatttttca cttgttgtga catattctaa acctcatttt ccttctcttt
     6001 cagatttgcc aaaattcttt cttgaaaacc ttcaaattgg tgaatttctt cctctgaaaa
     6061 tcctttgtaa aagatagtat ccaatttctg actgacacga tgccccactt ctttctggga
     6121 cttgcctaac tccgttaaaa ctaaatactt cttacgcttg tcttttccac acggactaac
     6181 aattacaagc ttttgttcct ctagcttttt tatcatagtc gtcagcgtat tattcgcaag
     6241 cccagtcgca agcgcgatat ctgtcgcagt tgcgcagcca gtttcactat tccataaaac
     6301 cgctaaaatc ttgccctgtt cacccctata aagagcctca ggatcttgac tcagtaactt
     6361 ttgaaaaatc cgcccattca acaaacgaat atgatgggct agcaaatgac catctttcat
     6421 aacacctcca atttatttcg atatcgaaat gaataaaaca attgtaacac tcatcgttct
     6481 aactgtcaac tatttcgatt tagaaataat ttttgataat tatccacacc accatactcc
     6541 ggctcaacta acttttaacg agagtttcta aactccttcg tcctccagtc tacaaaagcc
     6601 ttccattcgt actatcctat attttatgag gggacacatt tttcctatca gaccatttat
     6661 tttaaagata gaagtaaatc ataattgctt ccatctgttc ttttatagta tattgaagtt
     6721 agactagagc actgtatctt ctaaaacatt gatagaaagc gatttgaatt tcccaatcaa
     6781 tttgttcgta tttatagcat ttcgaaactg gaataggaca ccatgactgc taaaagattt
     6841 ctataaattc atttaatttc ctcaatcaat ttgttcatat cttatttcat tctgctataa
     6901 tttcacctta ccctatcttt ttcgtagcac ccttcaaaca gcctatcccc taccgtttga
     6961 cgattcctca cttcgctcca cttccattac agaagtttct tcactactat ggtctcggct
     7021 gacttctcat gattccttgt tactactatt tgaacgctca cgagataggt cttacaaaaa
     7081 atgctttgat ccacaatgga atcaaagcat tttaaagagt tcctcataca taagcgcaga
     7141 agtcgcagtt cctctgtact tggcttcttc tcttttgata aagcgagcca agttgagcaa
     7201 ctcaggtgct ggatgtttgg gatttaggag caattcacga ttgaccaggc ctgagagacg
     7261 aactgcctgc aattgctcat ttgtagtagg cagtttttta gcagtctcta ggagagcagc
     7321 aactaaatct tcactcaaat catgtcgagc atgattgtaa agatctttta taaggctttc
     7381 taggtttggt tctaccatcc ctaccacctc ccttatggtt taataatgtt taatcaaatc
     7441 aaccgttgaa cgatccaatt tcttcaccaa ggcttgtaag aaagcttgcg cttctaggaa
     7501 gtcatccatt gcatagaggg tttggtgaga atggatataa cgagcgcaga caccgatagt
     7561 tgttgatggg acaccaccat ttttcagatg agctgcacct gcatctgttc cgcctttacc
     7621 acagtagtat tggtacttga taccagcttc ttcagccgtt gtcaaaagga aatccttcat
     7681 ccctgggaga agcaagtgac ctggatcata gaaacgaatc aaggttccat ctccaatctt
     7741 gccttgacca ccgtagacat cacctgctgg tgagcaatca actgcgagga agacttctgg
     7801 gtcaaacttg gttgtagagg tatgagcgcc acgcagacca acttcttctt ggacgttaga
     7861 acccagatag agttcattgc cgagtttttg acctgataaa gcttcagcta gctcgcttac
     7921 catgaggaca ccgtagcggt tatcccaagc ttttgagatg atattttttt cattggctgt
     7981 caaaattgca gaactatctg gtacgatggt atcaccagga cggatgccaa aactttctgc
     8041 ctcagccttg tccgcaaaac caccatcaaa aacgatatcg gcaatggctg gcatggttgg
     8101 tcccccattt ccacgagtca aatgcggagg aacagaacct gaaatcacag gaatttcatg
     8161 accatcacga gtcaagagtt tgaaacgttg gctgctaacc accatggggt tccagccacc
     8221 gatttctacg acacggaagg taccatctgg cttgatttcg ctgaccataa aaccaacttc
     8281 gtccatatga gaagcgacca agacgcgcgg tgcatccaca gcttctgaat gtttgatacc
     8341 aaaaatacca cccaagccat ctgtcaccac ttcatccaca tgcggtgtca acttttcacg
     8401 aagataagca cggacaggcg cttcatgacc tgagactgca gcaagttctg ttacttcttt
     8461 aatttttgaa aataatgttg tcatttcagt tccttctttc tttcatccat tttaccactt
     8521 tttataggag aaggatagtg ggaaggtgga tttctaagtt agtatcttag tcctgctcta
     8581 tcttagaaaa ggatagtatt ctcttgcatg tagtgcaaaa tctagtaaac attccaaaat
     8641 taactcgaat atttatttcc aaacaaaaaa acaatacacc atcaaagttg tttggatttt
     8701 tcatgaaatt tacagaaaat agttgacttc cctttcttct ttctttaaat
//
LOCUS       DAVMEF010000074         8709 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMEF010000074 DAVMEF010000000
VERSION     DAVMEF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.074"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(112..276)
                     /locus_tag="VYQ30_001254"
     CDS             complement(112..276)
                     /locus_tag="VYQ30_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1966360.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(357..3008)
                     /locus_tag="VYQ30_001255"
     CDS             complement(357..3008)
                     /locus_tag="VYQ30_001255"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1966361.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVLLPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFDQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKNLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(3001..3579)
                     /locus_tag="VYQ30_001256"
     CDS             complement(3001..3579)
                     /locus_tag="VYQ30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1966362.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(3579..4139)
                     /locus_tag="VYQ30_001257"
     CDS             complement(3579..4139)
                     /locus_tag="VYQ30_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966363.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSELGVI"
     gene            complement(4126..4575)
                     /locus_tag="VYQ30_001258"
     CDS             complement(4126..4575)
                     /locus_tag="VYQ30_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1966364.1"
                     /translation="MTGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(4987..5382)
                     /locus_tag="VYQ30_001259"
     CDS             complement(4987..5382)
                     /locus_tag="VYQ30_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1966365.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(5478..5732)
                     /locus_tag="VYQ30_001260"
     CDS             complement(5478..5732)
                     /locus_tag="VYQ30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1966366.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(5742..7076)
                     /locus_tag="VYQ30_001261"
     CDS             complement(5742..7076)
                     /locus_tag="VYQ30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1966367.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEVFEAFAIEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREETKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(7076..7309)
                     /locus_tag="VYQ30_001262"
     CDS             complement(7076..7309)
                     /locus_tag="VYQ30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1966368.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(7724..7906)
                     /locus_tag="VYQ30_001263"
     CDS             complement(7724..7906)
                     /locus_tag="VYQ30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966369.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(7908..8687)
                     /locus_tag="VYQ30_001264"
     CDS             complement(7908..8687)
                     /locus_tag="VYQ30_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966370.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT         2463 a         1992 c         1668 g         2586 t
ORIGIN      
        1 attgtttccc atccacttca tctatttgat caacatttgt actaaaccaa tattcagaca
       61 cacttttatt ggctggagaa ccatataaag cttgtaaaat tgaagtttta tttactccat
      121 atctattaca gacacctcag gattatttaa cttataagtt ttaacagcta cggaatcaat
      181 ttcaacagca acttgaacat ctatgcctga ttttttaagg ccacttgtag tgccacctgc
      241 accgttaaat aaatcaatag caacaatttt ccccatagta ttctcctaaa gtttctcctt
      301 tttattataa cattatcaaa tgtaaaaccc aacccgatag ggttaggttt ttaacatcat
      361 ttcaccaact tcttcatctc atcaatacgt gctacggtcg catcatactt ggcttggtag
      421 tcagcttgtt tgtcgcgttc tttttggacg acttctggtt tggcgttggc tacgaagcgt
      481 tcgttagaga gtttcttacc aaccatgtcc agttcttttt gccatttagc aagttccttg
      541 tcgagacggg ccagttcttc ttcaacattg aggagatcgg ccagtggcag gtagatttct
      601 gctcctgtga tgacactcga catagccagt tcaggtgcag ggatggttga tgcgatttcc
      661 aaatgttctg gatttgtaaa gcgcttgatg tagttgacat tgctgttaaa gaaggcttcc
      721 aagtcactat cgctagtctt aacaaggatt gtgataggtt tgcttggtgc tacatttact
      781 tccgcacgcg cattccgaac agcacgaatc aagtttttga gactttccac accagtgtgg
      841 gcagcaaggt cttcaaaggc tgggttgaca gttgggtatg ctgctgtcac gatagagcct
      901 tcagcgtact gatcaaagat ttcttctgtc acgaacggca tgattgggtg aaggagacga
      961 aggattttat ccagcgtata gaggagaaca gatcgagtaa ttaccttatc gtcttcattg
     1021 tcgctgtaaa gaacttcctt ggtcaactca acataccagt tggcaaattc ttcccaaatg
     1081 aagttgtaaa ggatatgacc agccacacca aactcaaact tatcaaagtt ttcagtaact
     1141 tttgcaatgg tttcgttgag attgtggaga atccagcggt ccgtcacatt accagcctca
     1201 cctgttgcaa cttttgtgac attgtcatgc gccacatcca gcgtcaaacc tccattgttc
     1261 atgaggatat agcgagaaat gttccaaatc ttgttaataa agttccatga agcatccatt
     1321 ttctcgtaag agaaacgaac gtcttgacca ggagctgagc cgtttgacaa gaaccaacga
     1381 agactatctg taccatattt atcaataaca tccattgggt caatgccatt tccaagagat
     1441 ttagacatct tacgtccttc ttcatcacga attagaccat ggataaggac gttttggaat
     1501 ggttgacggc ctgtgaattc caatgactgg aagatcatac gagacaccca gaagaagatg
     1561 atgtcgtaac ctgttaccaa ggttgaagtt gggaaataac gtttaaagtc ttctgagtcg
     1621 acttcaggcc agcccatggt tgaaaatggc cagagggcag aactgaacca agtatccaag
     1681 acgtcttcgt cctgagtcca tccgtcacct tctggagctt cttcgccgac atacatttca
     1741 ccatcagcat tgtaccaggc agggatttgg tgaccccacc aaagctgacg agagataacc
     1801 cagtcgtgga cattttccat ccattgaagg aaggtatcgt tgaaacgagg tgggtagaat
     1861 tcgaccttgt cctctgtgtc ttggttggca atggcgttct tagccaattg gtccatcttg
     1921 acgaaccatt gagtagacaa gcgtggctca actacgacac ctgtccgttc tgagtgaccg
     1981 actgaatgga cacgtttttc gattttgaca agggcaccga tttcttccaa cttagcaacg
     2041 actgccttac gagcttcaaa acgatccatg cctgaaaatt caaaggcaag ctcattcata
     2101 gttccgtcgt cgttcatgac gttgacttgt ggcaagttat gacgttggcc aaccaagaag
     2161 tcatttggat cgtgggcagg tgtgattttc acgacaccag taccaaactc aggatctgcg
     2221 tgctcatctc caacgattgg gatgagttta ttagcgattg gaaggaggac gtttttacca
     2281 atcaagtcct tgtagcgcgg gtcttctgga ttaaccgcaa ccgcaacgtc cccaaacatg
     2341 gtctcaggac gagttgtagc aacttcaagg gcgcgtgaac catcttccag catgtaattc
     2401 atgtggtaga aggcaccttc tacatccttg tgaatcacct caatatcaga aagggctgtg
     2461 cgagctgctg ggtcccagtt gatgataaac tcaccacggt agatccagcc tttcttgtaa
     2521 aggtccacaa agaccttacg aacagctttt gacaaacctt cgtcaagagt gaaacgctca
     2581 cgagagtagt ctacagagag ccccatcttg ccccattgtt ccttgatagt agtggcatat
     2641 tcgtctttcc attcccagac tttcgtcaag aaagactcac gaccaaggtc atagcgggaa
     2701 atgccctcac cacgcaagcg ctcctctacc ttagcctgag tggcaatccc tgcgtggtcc
     2761 atcccaggaa gccaaagggt atcaaaacct tgcatgcgtt tttgacggat gataatatct
     2821 tgcaaagttg tatcccaagc gtgaccaagg tgaagtttac ctgtaacgtt tggtggtgga
     2881 ataacgattg aataaggctt agccttttga tcgcctgaag gcttgaaaac atcagcatca
     2941 agccattttt ggtaacgacc agcctcaacc tcggctggat tgtatttagg tgaaagttct
     3001 ttagacatgt gtgtgtcctt tctctatttt gtttatttta ttttgaattt gcttagcagc
     3061 ttcttctgca gacaaattcg tattatttat tttaaagtag tggtgcaact cattcggttg
     3121 atattgggaa tttaattgaa gtgtttcagc ggtctctaaa atttctcttt cagatacctc
     3181 aatatgtcgt tttaagggtt tgtgctttaa tcgattctcc gttcgatttc gacgtatgca
     3241 ctcttcaaga cttgtttcca actcaacaaa aagaatctct tgatgataat catccaacaa
     3301 attctgaatt tgctttaaat atatcagatg atactgattt gaaaaatcaa ttacatctgt
     3361 taaaattact gatcgctgat ttcttgcact tgctccaagg aatgaaaaag tgattccacg
     3421 aacaaattcc cacatttcct ctgtatagtc ctgatagatt tctagtgcaa aatcaatggc
     3481 ttgatggtta taaaataggg tagcatccgt cagtcgagat aattcttgac caatggtcat
     3541 ttttcctgat gctggagcac caatgatgaa aagatgcatc aaatcacccc caactcactc
     3601 ttcagcaagg catatctcaa atcatcacag cagttgcctt gagcatcttt acggtctctt
     3661 atgcgagctt cgagggtaaa gccaagcttt tccgcgactc gttgactttg aaggttatat
     3721 ccaaagcaag ttagttcaat cttgtgaaga cccaaatctt taaaggctaa gtcaatcaag
     3781 gcacgcgcag cttctggcac ataacctcgc ccccaatagt ctgagtgtaa ggtatagccg
     3841 atttccatca catcatcttc atggcgatgg ttgaaataga tagagccaac gatcttatcc
     3901 gttcctttga cgacaatccc gtagcctgct gggagatttt ccttttgatt acgctctgga
     3961 agaatgtgct ccagataata aatctcatct tccaaggtct tgactggagg aaaacctgct
     4021 ggataggcga cctctggcaa actagcatag tcaaagatat cctcagcatc cgccacagta
     4081 cggactcgta aaacgagacg ttctgtttcg attttatctg gcagctcagt tcttgccatc
     4141 cttcttcctc gcttttttga tgaaactgcc cttcatatct acacgcttgt ccagatagcg
     4201 ataaacgcgc tgatatccat ctcccatgaa ataggttggg gcaaacagtt gatttttaaa
     4261 atgtcccttt tcatccagga gttctggggc aacaagtcgc tcaagaatct tggcaaagat
     4321 gtggcaaata ccgtcttcct caacaatcct atctacccga caatctaaaa caagtggaca
     4381 ggcgtctaaa ataggagtct gagttcgttc agaaatttca taatgcactc ccaaacgttc
     4441 caatttctcc tgatgactga taaaaccagc ctgctccatc gcaagcatag aagtttcatc
     4501 agaaatattc acagtaaatt tttgatactg tttgatctgc tctgcggcat tctctctcgc
     4561 aacgactcca gtcacaaccc aatctcctag actataagag gaactacagg tcgtgatgtt
     4621 atagccaaaa ttctaatctt gatatcctaa aataaaaaca ggaaaaccat aatatagttt
     4681 attagtttta aatgattgtt ttataacaac ctcctttgac taagacacaa aagaaaagcc
     4741 ctgccatcta catgacaggg acgaatgtgt ttatccgcgg taccacccaa tttcgggcca
     4801 agcccgcatc tttagtcttt aaaataaaaa gacagaaata tttcattttt tcagtcatta
     4861 gcaaccagtt ttgacacatt tgtggacttc tcagcgccgc cactttctgt aaaatgtggg
     4921 attcaaaaca cctctaatgg ctctattgta gcaagttttt ctcggaaatg caaggctcaa
     4981 gaaaaattac ttgaacttga taccattttc tttcaatttc ttgatggtag ctgggccgat
     5041 tcctttcaag gcaaggagtt ctttttcagt ccagtttttg aaatctgaag cagacttgat
     5101 tccttcatca tagaaagtct tggcacgatc aagtggaagt ccacccaagt ttgctgcaaa
     5161 atcttctaca gaattagcta ctttttgagc ttgctctttg gtagcttcta ctgcttgttc
     5221 aactttttta gaaacagcct taccagctgc gcttgctgac tgttctgcac gcttcacgac
     5281 tttttttgtc tcatctacaa ccttagtcac agtacttgaa aaggcacctg cgcgacggag
     5341 gccatttcgt aattgttttt tacgattgag tttctttgac atgataaaat cctttcaata
     5401 aaaaatccct gctctgacaa ggatttaaca taaacattct atcaagattt tagtgaaaaa
     5461 tcaagaatct ttctcgttta ataatttcgc tcaccaaaca agccttctgg caaatcatgg
     5521 aatcccttgc ctactttgaa gttttcaaac ttaccaagca agaactgata agcatccaag
     5581 cgactctcgt agcgaatcat ggcatctttg gcacgctcgt cctggtcttc ttcgtaaact
     5641 ttttgacttt ccttgtgcgc catatcgtta atcatgattt gggtattgac ccaagcttca
     5701 aattccttca taaattcttg ttcgtaactc attttttctc cttattctaa tccacgaaaa
     5761 tagtccgtat caatctcacg gtagggctgg atatcctgaa catactccaa cactccttgg
     5821 aattctccgt tttcatcgtg tactgcagca taggtgatgt ggacaaactt tcctcgcgac
     5881 tcagacttga accacatttc atacttgtcc ttggtctcct cacgaagtcc cttcatgata
     5941 gccttgacct tgtccaaata cttaggcgga tgacagagtt cgacattgcg cccgacttgg
     6001 gacggcgtcc gtttgaaaat catctcatca gctggcgtat tgtcattgta atactggaaa
     6061 atatcttctt tattgacaaa ggtaatctcc atagggagat gattgaggat gagattggcc
     6121 tgctcgactg aaagatagcc attaccaaaa gcctgttgac tatggcggtc cagcacagct
     6181 tccttttcct taggggtaaa ggtaatggta aaatggcctt ctggcgtatc tatgacttgt
     6241 tgaacttgac cttctgccgt atctagctgt acaggctcct ctgcaatctt ttcctcaata
     6301 aagctctgtc gttctggcac ccatttctct gacggacgga tgatggcata gccataggca
     6361 tcgctctcct ccgcaatctg aagccagtca tcctgagtaa aagactcaag gagaatcatg
     6421 aggaggatgg actcttcctt gaaaatcata ctttcaaact ctatcgcaaa agcttcaaaa
     6481 acttccttta cactgctaat tgacacttct ggtagtgact tggctgtcgt tagagctgtt
     6541 tgaaagagtt ccctaatctg atcatccact ccccacataa ctttgggagg tgaatcgtgt
     6601 ccatagcgct ccatgatagg aaagaagagt tcttctttac gttggtaatg gatgtcaaat
     6661 tgccccacaa gtcccatctg acgcaccaaa cccttacgca tctccgcaag catttcctcg
     6721 tcttccatca actcataggt atctaacaat ctacgaatgc gaatcaaagc tgcacggagg
     6781 gccaaatttt cttccttaaa gacacgaact gggtgacctg gatgctcagt atctgaaact
     6841 tcgacacctt tgatagcatt tttaaaaaga ttggcatgga catcacagag ttccataaca
     6901 tcttcaaagg tgacacctga gtctgagttc atcagctcgt gctccataag ggaaatctca
     6961 atggctgaga cacccgcaaa ggtcgcatca aaacgctcct gaaccgactc aggagaggcg
     7021 ccattatgca attctaataa aatatcccgt aggacatgaa tccgttcatc tgccattagt
     7081 ctaatccaat cacttcgtag ccattcgctt ccagtgtgcg tacaatcttg tccataggag
     7141 ttcctgctag cttagaaccc tgtttaagtg atactttacg accaactgta ttgcgcatta
     7201 agggattggc aaggggttta aaacccaact ccactagaat ttccaagact tctggatgct
     7261 tgtccaccac ttctgcaaca ggaattgaca catcgatgat attgtccatg acgacctcca
     7321 ttatatgttc taatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggttg
     7381 ctcaaaacag tgttttgagg ttgtggatag aactgacgaa gtcagtaaca tatatactgc
     7441 aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgat tttactgctt
     7501 atattatacc atatggagag agattaaaaa actagcagtg gagttcctgc tagctctctg
     7561 cgacttggat taccatctag ataagtttaa agagccaatc caatatctta aataatatct
     7621 tgtaaaacag caattgaact gcaaaagaaa tgatcatcca aaagaatttc acaatcccaa
     7681 taatcggttt gataaaaaga atagcaagaa gaccaaaaaa atttcattct ctctcctctg
     7741 gcttaatgag ccaccgagcc gtatccatta gcttatctgc aataaaaagt ttgcccagac
     7801 caacaacgac atggtcagct ttctttatcg ttttcatcac ttttacacct tgcacggtca
     7861 aaaagtaatt accataaatt ttagcaaaag ctttgatagg attcatatta ctctcctttg
     7921 atgatttcag cctcttttcg gattgtttca acatcttctt gatattgaac ttcactatag
     7981 ttgactagct tggataattc tttctgcaag ctttccccca ttggtttcat agttgcaaag
     8041 gttaaaccac ctgaaatgat accacccaag ataggaataa atttccctat tcctttggcc
     8101 agccctccct tggtaagatt cacaccaaat attcttaaga cttttttcaa aatagggtac
     8161 caaagcgtct ttgttaaagc tttattaggc actgttttca ttacctgttt ggcaattgtt
     8221 ataccaccag cacgtagcaa agcagcagtt ccattcaccc ccaacatgac gcctagataa
     8281 agcaaaaggg tattttgagc atcttcactc aactcatcgc gtgaagccca aagatcctca
     8341 taaccataaa tataacctaa ttcttgggcc aatttcagag agaaaccata aaattgagcc
     8401 acatcagctg gaatggtaat tgccatagca agccctccag gtaatcctgc caaaacagaa
     8461 gtcccactcg ctaataaaac attatcccta atacaggcat tagccactcg atctaagatt
     8521 tcttgagata agagagacgt tggaccttgt tccaataagg taggaatgtc ctgtggaccc
     8581 aattctttgg aaaacttatc cactaaaaat ttcgaacgat caaccttaac aacaggtagt
     8641 ttcaccactt gttgcaatac ttgcatagct aagtcttgtt cagccatata caaactcctt
     8701 atttctttt
//
LOCUS       DAVMEF010000075         8669 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMEF010000075 DAVMEF010000000
VERSION     DAVMEF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.075"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(54..515)
                     /locus_tag="VYQ30_001265"
     CDS             complement(54..515)
                     /locus_tag="VYQ30_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966371.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(482..625)
                     /locus_tag="VYQ30_001266"
     CDS             complement(482..625)
                     /locus_tag="VYQ30_001266"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1966372.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            894..1274
                     /locus_tag="VYQ30_001267"
     CDS             894..1274
                     /locus_tag="VYQ30_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1966373.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1373..1594
                     /locus_tag="VYQ30_001268"
     CDS             1373..1594
                     /locus_tag="VYQ30_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1966374.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1611..1925
                     /gene="trxA"
                     /locus_tag="VYQ30_001269"
     CDS             1611..1925
                     /gene="trxA"
                     /locus_tag="VYQ30_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1966375.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2230..2763
                     /gene="queF"
                     /locus_tag="VYQ30_001270"
     CDS             2230..2763
                     /gene="queF"
                     /locus_tag="VYQ30_001270"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1966376.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYDQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            2825..3037
                     /locus_tag="VYQ30_001271"
     CDS             2825..3037
                     /locus_tag="VYQ30_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966377.1"
                     /translation="MFEVIDKTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSNH
                     TYGKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(3062..3730)
                     /locus_tag="VYQ30_001272"
     CDS             complement(3062..3730)
                     /locus_tag="VYQ30_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1966378.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGVVFFLLGN
                     SDMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLNITGLSVNPARSLAPAVLVGGTALQQVWIFILAPIVGGVLAALVAKNFL
                     GTEE"
     gene            complement(3841..4632)
                     /locus_tag="VYQ30_001273"
     CDS             complement(3841..4632)
                     /locus_tag="VYQ30_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1966379.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAKGVSSDGGLDKEIDKVGLPTLERSFRALIYANLLSADANQ
                     QSVFYQELNAGFRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLKEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            complement(4878..6674)
                     /gene="pepF"
                     /locus_tag="VYQ30_001274"
     CDS             complement(4878..6674)
                     /gene="pepF"
                     /locus_tag="VYQ30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1966380.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEATFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(6685..7428)
                     /locus_tag="VYQ30_001275"
     CDS             complement(6685..7428)
                     /locus_tag="VYQ30_001275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1966381.1"
                     /translation="MQQYFVKGSAISPVTIDNKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7430..8380)
                     /locus_tag="VYQ30_001276"
     CDS             complement(7430..8380)
                     /locus_tag="VYQ30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1966382.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHGLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
BASE COUNT         2556 a         1989 c         1659 g         2465 t
ORIGIN      
        1 ataaatcagc taatttttgg tttacattcc tatttttatg cataattatt ctgtcattct
       61 ctaatttgtg aatcaatata tctgattaga aaactaccat tgagtttttg ctccacctca
      121 ttagtccgct caaaacgccg tccgctaatc ttgtcataga ctttctcccc tctaggagcc
      181 cctgcataag gacgatagag gtagcggttg attcccgttt cgtcaatata taggactggt
      241 aaatctttta ggttatccaa aatatcaaga aactcagaaa ctttctcagg cttttgttcc
      301 ctaaaactgg tcgtcttttt taaaatgacc ttaatctgct ttaaaactgc ccatacggag
      361 ggcaaagcac aatcaaaacg ggccgcaatt tcccgtaaaa aagcgtctgg atgagcctct
      421 acaaaggctt tcaattcttc taaagggatc ttacgctttt tgacgactcg ctttttccgc
      481 tctaagttcc cttgctcacg tctttctttt cccacgtgaa gagagttctg acgccgacat
      541 caaaaacttt ggccgcctcg acatgtctgt gccctccttt gatggaatct aatgctcctt
      601 gtttaaaatc agtactatat gccatagctt tattagacca tgcttatttt aaactaagca
      661 actatagata gagaaatgaa ataaaatctg aattcgaatg tgataaggag ttagaaattg
      721 aaacaatagg attcccccat tattcacaca aaaataaaac cactaaaaaa cagagcttgc
      781 cagcattctt gaaaagtagt ataattattg catgcgcaat catcttgtga cgcagagact
      841 gcccgttaat ggactttctt ctatattatg actctaaaaa gaaaggagac actatgacct
      901 atttggaaaa atggtttgac ttcaatcggc gtcagaaaga aattgaaagt ctcttggaag
      961 agaccattgc ccaacagagc gagcaaagtc tgaccttgaa agagttctac ctgctctact
     1021 atctggactt ggctgaagaa aaatctctac gccagattga cctgccagat aagcttcatc
     1081 tgagcccgag cgcagtttct aggatggtgg ctcgcttgga agctaaaaat tgcggtctac
     1141 tcagtcgcat gtgttgccat caagatagac gctctagttt tatctgcctg acaaatgatg
     1201 ggcaaaagac actggcctcg ctacaaaaga ctgtcgaaga aagcttggaa gcaggtttgg
     1261 atttcttgat ttaagatgat tttaaaaaaa gttgcgtgcg caatcatttt tcttgacatt
     1321 ctcttttata aggagtaaaa taaagttagc attaaacaaa ggagttttaa atatgattga
     1381 aattacctat atagatgcca gcaagaacga aagaactgtg actttcgagt cttatgaaga
     1441 ctttgaacgt tcgcaacaag cttgccttat cggcgtcgca gactactacc ctgtccaaaa
     1501 attaacctac aagggtcata atttggacta ccatgggact tatggagata tcttcttcta
     1561 tctcatgaaa caagatttaa gccaatataa ctaaaaaaag gagaaataca atggcaaaag
     1621 caattacaga tgcaacattc gaacaagaaa caaaagacgg tttggtctta gtagacttct
     1681 gggcaacttg gtgtggtcca tgtcgtatgc aaggtccaat cttggacaaa ttgtctgaag
     1741 aactttcaga agatgtcttg aaaatcgtta aaatggacgt tgatgaaaat ccaaacacag
     1801 ctcgtgcttt tggaatcatg tctattccaa ctcttctctt caaaaaagac ggccaagttg
     1861 tcaaacaagt tgcaggtgtt cacacagcag aacaaatcaa ggccatcatt gctgaattga
     1921 gctaatcaaa cgagagacca agtactttct ttggtctctt ttttcttgcc ctttgctatt
     1981 tttcaaaaaa tatgctagac tatagcagat tgaaactaga atagtacacc tctacttcta
     2041 aaacattgtt agaaatcgat ttgattgtcc tgatcgattt gtcctgttct tatttcattt
     2101 tactatataa gtagaatatt acgatgtttg ggatatgcca tcgctaaaaa aactcgattt
     2161 ggtatttttt agctccctaa tgggagcttt ttgcgtgctc tgaacatttc ccattttaga
     2221 aggagtacta tgaaacgtca atcagccttg gtcgtcttta gtggtggtca agattcaaca
     2281 acctgccttt tttgggtcat gcaacactat gaaacagtcg aagctgtcac ctttgcctac
     2341 gaccaacgtc atcacctcga aattcaaatt actagagaaa tcgctaagga acagggcatt
     2401 cgtcaccata tcctcgatat gtctctgctg ggacaaatca ctgctcagcc agactttgcg
     2461 acgattcata tttcctacat tcctgacaag ctctgtgtcg agtcaaaatc cctcaaacta
     2521 tatctattta gctaccgaaa ccacggagat ttccacgaaa actgtatcaa caccatcggg
     2581 aaagacttgg tcaacttgct agaccctcgc tatttagaag tctggggaaa attcactccg
     2641 cgcggtggca tttcaatcga cccctactac aactacggta agcaaggaac taagtatgag
     2701 ggcttggcag aacaacgcct cttccaacac gacctttatc cagagaaaat tgacaaccgt
     2761 taaactcata ctcaatgaaa atcaaaaagc aaactaggaa gctagccaca ggctgctcaa
     2821 agcagtgttt gaggttatag ataagactga cgaagtcagc ttaaaacact gttttgaggt
     2881 tgcagataga actgacgaag tcagcttaaa acactgtttt gaggttgcag atagaactga
     2941 cgaagtcagt aaccatacct acggcaaggt taagctgacg tggtttgaag agattttcga
     3001 agagtatcat acgaaaaagc cctgttcctc aagatgagga gcaaggcttt ttgagtttca
     3061 attattcttc tgttccaagg aagtttttcg caacaagggc tgcaagaacc ccaccaacga
     3121 ttggggcaag gatgaaaatc caaacttgtt gaagggctgt gccacctacc aagacagctg
     3181 gtgccaagct acgagctggg tttactgaaa gtccagtaat gttcaatccg acaagaatca
     3241 tcgccatcaa ggacaaaccg attaccaaac cagcaatcgc gccatttccc ttgctttctg
     3301 aagtcacagt catgataacc aagacaaaca agaaggttgc gatgacttca aacaagaaac
     3361 caccaaagac agtgacaccg tttgccaagg cattttcacc aaggctagca gttgacatat
     3421 ctgaattacc caagaggaag aagacaacgc cagaagcaat gaaagctcca actacttgcc
     3481 caaggatgta gtttacaagt tctgaagatg acaagcgttt gtttacaaac atagcaatag
     3541 aaacagctgg gttcaagtga gcacctgaaa cagttccgat tgagtaggct gcaaccacga
     3601 ttgctaaacc aaaggcaaag gcgattccaa ggtgacctag gccatcaaga ccatttccaa
     3661 aaacaacagc tccagtccca acgaacacaa gcatgaacgt accgattagc tcagcaacaa
     3721 attttttcat tttctttctc cttttttcaa aaactagata ctagtctatc aaaagtagga
     3781 aagggtttca agaaaattga ttggaaattt tttgaaaatc atagaactat tagctaatcc
     3841 ctagtattga aaagactgga tagcttcttt caggtcatct tgtaaactat ttctctggtc
     3901 aagttggaca tagacttcca ccagacagga tctaaagttg gaaaatttgt aaaaatcctc
     3961 cctttcttct atcggaaaat caacagtttt tatccaagaa gctacttgtt cttgctccaa
     4021 cttcccttgt aaaataggtt catagatcac tcttgctaaa cgccaatcct catcatctgt
     4081 aaagcgaatc gacattcttt taaatagttg gccaagtata tcaaatactt catgaactct
     4141 gtttttagga aagtctggat gacaaaccac ctctttcagt aaatcggctc catgtgcaaa
     4201 agcgtgaacc caaccatact gacttgagaa acctgttgta tccttttctt ttgaaagata
     4261 gtgcaagcct tgatttaaaa ggacattacg aaatcctgca tttaattcct gataaaaaac
     4321 cgattgctgg ttggcatctg cagacaagag atttgcataa ataagtgccc taaaagaacg
     4381 ttcaagtgtt ggcagaccta ccttatcaat ctctttatct agtcctccat cagatgaaac
     4441 ccctttagca atgaaatgaa attgctcctg tgtaaatagc tcttcctgaa tccctctagc
     4501 aaagcttgta aaaacaaggt catcacgaat ttctggagaa ggatctccca aatgatcaag
     4561 caaccactgg atttcctcct ggttataatt tggtttttct tctgctattt ttcttagtaa
     4621 atcttgatac atggtcaata cctctacatt tctagcaact gttcaaaaag gcagtcttaa
     4681 atgactcaat attgaattct caattaaata caatctgata taaaatgacg taaataacta
     4741 tcaataccag ttctacagta agttcaaatt taacatcacg accttcaacg acatttttga
     4801 aaatagctac aactaagaca aatagaatga cgcttaacaa gcccataaac atcattctaa
     4861 aaaatttttc tattccccta ctctcccaac tcagcactat aggagataat ctggtcaact
     4921 gtgtcagaca agaattggat ggtatcacgg agtggtttgt ctgttgaaat atcagcaccg
     4981 ataatcatgg ctgactcaag tggtgtcttg ctaccacctg atttaaggag attgagccag
     5041 tcttcagctc cagtttcaga atgttttaga tgaaggtaac cagcagtcga gataactagt
     5101 cctgctgagt aagtgtaact atacaagccc atatagtagt gagcttggcg catccaagtc
     5161 aaagctgcat cgtcgtcgat ttcaatggca tcgccccaaa aatctgtcaa aacttccttc
     5221 ataatgctgt tgagcttgct tgctccaaag gtctcccctt cttcaatcaa tgtatacacc
     5281 ttacgctgga aggtggcttc caagaggtgg gtgatgaaat tatggaagta ggtgtctgtc
     5341 aagcgatgag ccagagcgaa gcgtttttga cgtgggtcat tagactggtt ctctaagtaa
     5401 tcactgagta gcaattcatt gaaggttgac ggtgcttcaa cataataggt cgacatgtgg
     5461 gcgttgaaat agctttgatg attgtctgaa aagatgaatt gaccagaatg cccgatttca
     5521 tgaatcaagg tatagacatc gctcaaacgg cctgtccagc tcatgagtac ataagggtgt
     5581 acgcgatatg ggtccgccgc ataaccacca gaatccttgc cactgttagc agcaaagtcc
     5641 acccagcgct cttcttggta acgagcaact tcctgacaat attcttgccc caaaggttct
     5701 accgacttca tgaccaaatc ataggcatcg tcaatagtca cttcaggatt cagggcgctg
     5761 tccaagtcca atttccagtc tgcaaaggtc atcttttcaa gaccatttac cttggcaaca
     5821 tgcttgaggt atctctgagc gactggtgca aagtccttca tgatgaggtc aatctggcgg
     5881 tcaaacatga cacggtccac ttcttgttca gctagaagat agtcaaagac agagtcgtat
     5941 cccttcatat cagccaagag tttttcagac ttgacctgag ccagataggc tgctgcagcc
     6001 gtattttggt gcttacgaag tccctctgag aaggaacgga aggatttctc acgaacctca
     6061 gcatcctcat ggttttggta gaaattctca taggtcacaa agctgttttt gtaggtcttg
     6121 ccatgggctt caaagtcagc catttcaaaa tccccagctc gcatcttagt ataaatgtcc
     6181 tgcggactgt aaaaaacttc accgagattt gtcaaggcct tctccacatc tgcccctaag
     6241 tagtgggctt ttttgatttt agcctgacga atggcagctg ttaaatgtgg caatttaccc
     6301 aaacggtcca agacttcctc gtctgctgcc accaaggcat cgtcaaagaa ggtcaaggct
     6361 acgctggcag ctgtttcaaa ttccatccca gcttgggcaa tattggcaaa ttcgtcattg
     6421 ctatagtccg tcgtctgagg cataaaagca tagttgccaa tatggctcat ctgaatgtag
     6481 atctgttcca attccgcaaa ggccttctcg aaatcctcaa aagtgtgaag attgcccttg
     6541 taatcacggc taaactggtt gatgtcttcg cgagctttct cgattgcacg caagaaatcc
     6601 tcacggtctt ggtatagggc tgttaagtcc cagagttcct tctctggaaa ttctgaacgg
     6661 tgtttttgtt ccattttctt cctcttattt ctctaattct actaaaacac taagggctga
     6721 taaagcgtaa agcggcgctg tttctgctcg caaaatacga ggacctaggc ctgccaaaac
     6781 ggctccttta gcttcaaaac tttcgatttc tgcaggtgag agaccgcctt ctggaccaaa
     6841 gataaagagc agtttggctc ctgtttcaag accagtgact gcttgcagaa gcgcagcggc
     6901 ttctccttct ttagctgatt cttcataggc tactatgata gagtcaaact ggtccagctg
     6961 agctagaaaa tctgcttttt tctcgaaaag tttaatactt ggtacaatat tacgcttgct
     7021 ttgctcggct gctccaaggg caattttttc tagtttttca acttttttac ccaatttctt
     7081 gccatcccac ttggcaactg accagtctgc aggaaaggcc cagatttggc tagcacccag
     7141 ttcggttact ttttgagcga tgaactccag cttgtctccc ttgggaaatc cagatgcgat
     7201 ggtcacttgg actggtagtt ccacattgtc atttaattct tggaccaact caaactgacg
     7261 attttccata tccagcacgc gcgccaagcg cttgatgcca tcatcaaaga ctaaggtaac
     7321 ctcatcctct tctttcaagc gcataacctg aaacatatgc ttactggttt ccttgttatc
     7381 gatagtgaca ggagagatag cactgccttt tacaaaatac tgctgcatgc tagcctccaa
     7441 tcacaccaga gatatccttg gttttcttaa agacacaggc attccattcc ccttgaacca
     7501 tgtgagtttc gaggaaaaat ccagctgatt cagccaactc gcgcaccatg tcccacttat
     7561 ccttgataat gccactcatg atcaggtagc cttcgtcctt aaccaaacga taggcatcgt
     7621 ctattagatg aatgaggata tccgccaaga tattagccac aatcacatct gcctcaattt
     7681 ccacaccctt aagcaaatct ccagccgcta catggatatt ttccatgcca gggttgagct
     7741 caatattttc ctgagccaca cgaaccgcca catcatccag gtcataggcg aaaatttctt
     7801 tagcccccag aagcgagctg gcaatagaga gaacccctga accagtcccc acatctagca
     7861 ccgtttcgcc accacgaaga acctgttcca aggcaaaaag gctcatcttg gtagttgggt
     7921 gggttcctgt cccaaaggcc ataccaggat ccagcttgat aatcttttct cctgctgttg
     7981 cctcatagtc cgtccatgac ggtacgatgg tcaggccatg ggtgatgcgt gccggttcat
     8041 aatacttctt ccagttgtca gcccagtctt cctcagccaa ggcagtcgtg cccatcttga
     8101 cttctcctag gtccataaag tcggtcaatt ctgccagacg agcctgcaaa tccgcttcaa
     8161 ctgctgctac atcaaccgtt tcagggtagt aggcggtcac tacgatttct tcctgttgct
     8221 ccacctctgg gaaaatctcg ccaaagcggt caacatttcc cacatagtcc atactgtctt
     8281 caatcgcaac accttgcgct cccagctcaa tcaagagatt ggaaaccaac tcctctccct
     8341 cacgcttcac tgtaactttt aactcttgcc atgtttccat tatgtatttt gcttaccttt
     8401 ccatcagatt aaaaatagtc tttccaataa cgacaataag gttgtctctt aattttttct
     8461 ctaactctaa gtctatacca ctattctgta tgaatttatc ttctacattt ctatataggc
     8521 gttttgctct aatgggattc cccggatcaa aatgaggtac atagtggtaa attatgacag
     8581 gaacagaacc gctgattgat cgttcagcaa tctgactcca gatataccta tcagtttctc
     8641 ccaaagaaac cccatagaga ataatgatg
//
LOCUS       DAVMEF010000076         8633 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMEF010000076 DAVMEF010000000
VERSION     DAVMEF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.076"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(338..514)
                     /locus_tag="VYQ30_001277"
     CDS             complement(338..514)
                     /locus_tag="VYQ30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1966383.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(625..822)
                     /locus_tag="VYQ30_001278"
     CDS             complement(625..822)
                     /locus_tag="VYQ30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1966384.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(1077..2621)
                     /locus_tag="VYQ30_001279"
     CDS             complement(1077..2621)
                     /locus_tag="VYQ30_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1966385.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVSGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            2834..3136
                     /gene="gatC"
                     /locus_tag="VYQ30_001280"
     CDS             2834..3136
                     /gene="gatC"
                     /locus_tag="VYQ30_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1966386.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLCEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            3136..4602
                     /gene="gatA"
                     /locus_tag="VYQ30_001281"
     CDS             3136..4602
                     /gene="gatA"
                     /locus_tag="VYQ30_001281"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1966387.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQTTLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            4602..6044
                     /gene="gatB"
                     /locus_tag="VYQ30_001282"
     CDS             4602..6044
                     /gene="gatB"
                     /locus_tag="VYQ30_001282"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1966388.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            6173..6733
                     /gene="efp"
                     /locus_tag="VYQ30_001283"
     CDS             6173..6733
                     /gene="efp"
                     /locus_tag="VYQ30_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1966389.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            6755..7144
                     /locus_tag="VYQ30_001284"
     CDS             6755..7144
                     /locus_tag="VYQ30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1966390.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            7137..7559
                     /gene="nusB"
                     /locus_tag="VYQ30_001285"
     CDS             7137..7559
                     /gene="nusB"
                     /locus_tag="VYQ30_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1966391.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(7608..7985)
                     /locus_tag="VYQ30_001286"
     CDS             complement(7608..7985)
                     /locus_tag="VYQ30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966392.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(8347..8532)
                     /gene="briC"
                     /locus_tag="VYQ30_001287"
     CDS             complement(8347..8532)
                     /gene="briC"
                     /locus_tag="VYQ30_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1966393.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
BASE COUNT         2643 a         1936 c         1654 g         2400 t
ORIGIN      
        1 atgaagagtt tactagtgag gagcttcatt ctcctcaact acaagtataa tctatttttc
       61 gctaaatggg aagcttttcc ttaaagaaaa ccactctatc ccttacattt aggagagaga
      121 aagtgaggag tgtaagacaa gcatttcatt tgataagagg cttggagctg ttaggctggc
      181 gagccaaggc taagactagc cttggtttag ctaacaaaac agcttcaagg gctcctcgtg
      241 tcaaatgaaa tcaaaataat tgccctcaaa ctctccattt tttggggaca taatcacttt
      301 cgattgacag ggaattggaa gattttttat ttgtgtctta aatcaggtct tgcgtgacat
      361 tttctgctct ccacatgcca tcgttgatta acagaacacc agaattaaaa ttatgtgtat
      421 aaaaatcatc tctaactgca gctaagggta tagccgtcaa gtccaaatcc cacagctcat
      481 ctatcgattt tcttacaaca atatctgaat ccaaatacag tacacgagac tcgcttacat
      541 actttggaat aaaataccta aaaaagccgc atatgaaagt ccctcaaagg ggagacgata
      601 acctttcaga atattactgt caatctaaac attcacaatc tcactattca aagtctctag
      661 tcttttttcc atcaattgga accattctcg cggaaggtca tcattaaaaa cataaaactt
      721 aagattataa tgatgaacac aaagagattt tattgttgtt tcaactttat ccatataagc
      781 attatctgca cctaagacaa tcgctttttt ctcttctttc actctttatc tcatttcttt
      841 ttattcccat catatgtttc tacgtaacca ttattttcgc ccattcgttc gtaaaaccat
      901 accagtggag attttagatg aagtcccatt acggtttaca atttttacat tacgacacgg
      961 agttttacaa atcgatttca tttgccaaac gtagttagta aggcagttag ctagttcgcc
     1021 aaatagcgac tagcgtccaa caatttggaa ctttagttcc aattgttggt actgagtcac
     1081 atcttctcct ctaactctac gtctggatac ttgtccgcaa accagcggag ggcaaagtca
     1141 ttttcaaaga gaaagactgg ttggtcaaaa cggtctttgg ctaagatatt gcgacttgac
     1201 gacatccgtt catccaagtc ctcaggcttg atccaacgaa cggtcttttt acccattggg
     1261 ctcatgacca cttccgcatt gtattcgcct tccatacggt gtttaaagac ttcaaactgg
     1321 agttgaccaa cagctcccag catgtactca cctgtttggt aattcttata aagctgaacg
     1381 gctccttctt gcaccaattg ctcaatcccc ttgtggaagg atttttgctt cataacattc
     1441 ttagcagaaa ctttcatgaa aatttcagga gtaaaggttg gcagtggttc aaattcaaac
     1501 ttgttttttc caaccgtcaa ggtatcccca acctgataag taccggtatc gtaaaccccg
     1561 ataatatcac ctgatacggc attggtcaca ttctcacgac tctccgccat aaactgggta
     1621 acattagata gtttggcacc cttaccagta cgagggagat tgacactcat gccacgctca
     1681 aattcgcctg atacgatacg gacaaaggca atacggtcac ggtgacgagg atccatgttg
     1741 gcttggattt taaagacaaa gcctgagaaa tccttgtcat aaggatccac aatttcaccg
     1801 tctgttttct tgtgaccatg tggttctgga gcaaacttga ggaagatttc aaggaaggtc
     1861 tgcacaccaa agtttgtcag ggctgaaccg aaaaagacag gcgtcaattc tccagccaga
     1921 atagcttcct ctgaaaactc attcccagct tcatttaaaa gctcaatgtc atccttgact
     1981 tgctcgtaga aaggattgct accaaagagt ttgtccccgt cttctagact ggcaaaacgc
     2041 tcatcccctt tgtaaagctc taaacgttgg ttatagaggt catacaagcc ctcaaaggct
     2101 ttccccatcc cgataggcca gttcataggg tagctagcaa tgcccaagat ttcttccaat
     2161 tcttgcaaga gatccaaagg ctcacgaccg tcacggtcca gcttgttcat aaaggtaaaa
     2221 actggaatgc cacgatgttt cacaacctca aacaatttct tggtttgagc ctcgatcccc
     2281 ttggcagagt ccacgaccat gaccgcagca tccaccgcca tcaaggtacg ataggtatct
     2341 tctgagaagt cctcgtgccc tggcgtgtct aagatattca cgcgcttgcc gtcgtagtca
     2401 aattgcataa cagatgaagt aacagaaatc ccacgttgct tctcgatatc catccagtca
     2461 gatttagcaa aagtccctgt tttcttccct tttaccgtac cagcctcacg aatttcaccc
     2521 ccaaagtaga gtaactgctc agtgatggtt gttttccccg cgtccgggtg ggagataatg
     2581 gcaaaggtac gacgtttctt aatttcttct tgaatattca taagttctct ttctttgatt
     2641 ctctattttt cttgtttcaa tagctgagaa tgatttttac attggatttt accattcctt
     2701 tcaacactcc attatatcgg attttagcat ttttttcaat ttctatttct tttcacttcc
     2761 ccctccctta tttataggaa aatatggtaa aatagaacag actaaaaatc atcatttcac
     2821 gaaaggatgc aagatgaaaa ttacgcaaga agaggtaaca cacgttgcca atctttcaaa
     2881 attaagattc tctgaaaaag aaactgctgc ctttgcgacc accttgtcta agattgttga
     2941 catggttgaa ttgctgtgcg aagttgacac aactggtgtc gcacctacta cgactatggc
     3001 tgaccgcaag actgtactcc gccctgatgt ggccgaagaa ggaacagacc gtgatcgctt
     3061 gtttaaaaac gtacctgaaa aagacaacta ctatatcaag gtgccagcta tcctagacga
     3121 tggaggagat gcctaatgac ttttaacaat aaaactattg aagagttgca caatctcctt
     3181 gtctctaagg aaatttctgc aacagaattg acccaaacaa cacttgaaaa tatcaagtct
     3241 cgtgaggaag ccctcaattc atttgtcacc atcgctgagg agcaagctct tgttcaagct
     3301 aaagccattg atgaagctgg aattgatgct gacaatgtcc tttcaggaat tccacttgct
     3361 gttaaggata acatctctac agacggtatt ctcacaactg ctgcctcaaa aatgctctac
     3421 aactatgagc caatctttga tgcgacagct gttgccaatg caaaaaccaa gggcatgatt
     3481 gtcgttggaa agaccaacat ggacgaattt gctatgggtg gttcaggtga aacttcacac
     3541 tacggagcaa ctaaaaacgc ttggaaccac agcaaggttc ctggtgggtc atcaagtggt
     3601 tctgccgcag ctgtagcctc aggacaagtt cgcttgtcac ttggttctga tactggtggt
     3661 tccatccgcc aacctgctgc cttcaacgga atcgttggtc tcaaaccaac ctacggaaca
     3721 gtttcacgtt tcggtctcat tgcctttggt agctcattag accagattgg accttttgct
     3781 cctactgtta aggaaaatgc cctcttgctc aacgctattg ccagcgaaga tgctaaagac
     3841 tctacttctg ctcctgtccg catcgccgac tttacttcaa aaatcggcca agacatcaag
     3901 ggcatgaaaa ttgctttgcc taaggaatac ctcggtgaag gaattaaccc agaggttaag
     3961 gaaacaatct taaacgcggc caaacacttt gaaaaattgg gtgctattgt cgaagaagtc
     4021 agccttcctc actctaaata cggagttgcc gtatactaca tcatcgcttc atcagaagct
     4081 tcatcaaact tgcaacgctt tgacggtatc cgttacggct atcgcgcaga agatgcaacc
     4141 aaccttgatg aaatctatgt aaacagccga agccaaggct ttggtgaaga ggtaaaacgt
     4201 cgtatcatgc tgggtacttt cagtctttca tcaggttact atgatgccta ctacaaaaag
     4261 gctggtcaag tccgtaccct catcattcaa gatttcgaaa aagtcttcgc ggattacgat
     4321 ttgattttgg gtccaactgc tccaagtgtt gcctatgact tggactcact caaccatgac
     4381 ccagttgcca tgtacttagc cgacctattg accatacctg taaacttggc aggactgcct
     4441 ggaatttcga ttcctgctgg attctctcaa ggtctacctg tcggactcca attgattggt
     4501 cccaagtact ctgaggaaac catttaccaa gctgctgctg cttttgaagc aacaacagac
     4561 taccacaaac aacaacccgt gatttttgga ggtgacaact aatgaacttt gaaacagtca
     4621 tcggacttga agtccacgta gagctcaaca ccaattcaaa aatcttctca cctacttctg
     4681 cccacttcgg aaatgatcaa aatgccaaca ctaacgtgat tgactggtct ttcccaggag
     4741 ttctgccagt tctcaataaa ggggttgtcg atgccggaat caaggctgct cttgccctca
     4801 acatggacat ccacaaaaag atgcactttg accgcaagaa ctacttctat cctgataacc
     4861 ccaaagccta ccaaatttct caatttgatg aaccaatcgg atataatggc tggattgaag
     4921 tcgaactaga agacggtacg accaagaaaa tcggtatcga acgtgcccac ctagaggaag
     4981 acgctggtaa aaacacccat ggtacagatg gctactctta tgttgacctc aaccgccaag
     5041 gggttccctt gattgagatt gtatctgagg cagatatgcg ttctcctgaa gaagcctatg
     5101 cttatctgac agccctcaag gaagttatcc agtacgctgg catttctgac gttaagatgg
     5161 aagaaggttc tatgcgtgtg gatgccaaca tctcccttcg cccttatggt caagaaaaat
     5221 tcggtaccaa gactgaattg aagaacctca actccttctc aaacgttcgt aaaggtcttg
     5281 aatacgaagt ccaacgccag gctgaaattc ttcgctcagg tggtcaaatc cgccaagaaa
     5341 cacgccgtta cgatgaagct aacaaaacaa ccatcctcat gcgtgtcaag gaaggggctg
     5401 ctgactaccg ctacttccca gaaccagacc tacccctctt tgaaatttct gacgagtgga
     5461 ttgaggaaat gcggactgag ttgccagagt ttccaaaaga acgtcgtgcg cgttatgtat
     5521 ctgaccttgg tttatcagac tacgatgcta gtcagttgac tgctaataaa gtcacttctg
     5581 acttctttga aaaagctgtt gccctagatg gtgatgccaa acaagtctct aactggctcc
     5641 aaggggaagt cgctcagttc ttgaatgctg aaggtaaaac actggaacaa atcgaattga
     5701 caccagaaaa cttggttgaa atgattacca tcatcgaaga cggtactatt tcatctaaga
     5761 ttgccaagaa agtctttgtc catctagcta aaaatggcgg tggcgcgcgt gaatacgtgg
     5821 aaaaagcagg tatggttcaa atttcagatc cagctatctt gatcccaatc atccaccaag
     5881 tctttgccga taacgaagct gctgttgccg acttcaagtc aggcaaacgt aacgccgaca
     5941 aggcctttac aggattcctt atgaaggcaa ccaaaggcca agccaaccca caagttgccc
     6001 ttaaactact tgcacaggaa ttggcgaagt tgaaagaaaa ctagacagaa caaaaccagc
     6061 cctaaggttg gttttttctt ctctaccaac tcccaataac tattttggct ttatttccag
     6121 agtattttat ggtaaaatga agagtaataa tatttattaa agaggtaaaa acatgattga
     6181 agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct gacggcaaat tgattcgcgt
     6241 tttggaagct agtcaccaca agccaggtaa aggaaacacg atcatgcgta tgaaattgcg
     6301 tgatgtccgt actggttcta catttgacac aagctaccgt ccagaggaaa aatttgaaca
     6361 agctattatc gagactgtcc cagctcaata cttgtacaaa atggatgaca cagcatactt
     6421 catgaataca gaaacttacg accagtacga aatccctgta gtcaatgttg aaaacgaatt
     6481 gctttacatc cttgaaaact ctgatgtgaa aatccaattc tacggaactg aagtgatcgg
     6541 tgtcaccgtt cctactactg ttgagttgac agttgctgaa actcaaccat ctatcaaagg
     6601 tgctactgtt acaggttctg gcaaaccagc aacgatggaa actggacttg tcgtaaacgt
     6661 tccagacttc atcgaagcag gacaaaaact cgttatcaac actgcagaag gaacttacgt
     6721 ttctcgtgcc taatctctag aaagaggtca ttctatggga attgaagaac aacttggcga
     6781 aatcgttatc gccccacgtg tacttgaaaa aatcattgct atcgctactg caaaggtaga
     6841 gggtgttcac tctttttcaa acagatcagt gtctgatacc ctttcaaaac tttcactcgg
     6901 ccgtggcgtt tatcttaaaa acgtggacga agaactcaca gcagatatct atctctacct
     6961 tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc cagaaagctg tcaaagatgc
     7021 cgtccgtaat atggctgatg tagaactcgc tgctatcaat attcacgttg caggtatcgt
     7081 cccagataaa acaccaaaac cagaattgaa agatctattt gacgaggact tcctcaatga
     7141 ctagtccact attagaatct agacgccaac tccgtaaatg cgcttttcaa gctctcatga
     7201 gccttgagtt cggtacggat gtcgaaactg cttgtcgttt cgcctatact catgatcgtg
     7261 aagatacgga tgtacaactt ccagcctttt tgatagacct cgtttctggt gttcaagcta
     7321 aaaaggaaga actagataag caaatcactc agcatttaaa agtaggttgg accattgaac
     7381 gcttaacgct cgtggagaga aacctccttc gcttgggagt ctttgaaatc acttcatttg
     7441 acactcctca gctggttgct gttaatgaag ctatcgagct tgcaaaggac ttctccgatc
     7501 aaaaatctgc ccgttttatc aatggactgc tcagccagtt tgtaacagaa gaacaataaa
     7561 aagaaaaagt gtttgataga tatcaaacac tttttctttg cctcccacta tctaaaaaaa
     7621 tgataataga tataattgta aacaaaaatc cagataggtt ttgcatgatt gagaaagtta
     7681 aaaaaactat ggcagagaat cgttaatctc agattgtcgg tagaacgata aacaagggca
     7741 aaaaagaaac caatcagact ataatataat aaactaattg gatctctgtg agatagtatc
     7801 aaatggctaa tcccaaagat aaggcctgat aagatgacat ctaaatagta ctttgactta
     7861 ggaaagaagg tattcataaa ataccctcta tcaagagtct cctcaaaaac aggaccgatg
     7921 attacaagca ggacaaaaga taagatagtc gataaaaagg ttggttgtcc atttgaaaga
     7981 accacggtaa aatactcatc atgaatattc ctatggttaa tcaaatgagc atagcgtgcc
     8041 taaaaattac cgagaatctg ataaaccaca tacgttgcaa ataagtagaa gacaaatgac
     8101 cagttccagc tctttttttc aaagataaag agcatctttt tcttttttaa cctccaaatt
     8161 aatagaagga aacttcccac taataccatt gttaaaataa gagaatagac atcagctcct
     8221 aaccctaaaa tgatcgtcac atacaatcca attgtttgtg gtaaataggt agatagtaaa
     8281 ataataagca aaaatattcc aaattgtctt agttttttgt gtttctcatc gtactttttt
     8341 gaaagattac cctgctcgga agccgtactt ccaagcatct atataagaat taagtgcccc
     8401 ttgcctcata taaggagcaa attctctata atataaccat ctactatatc catcttccca
     8461 aacagcaaga ccacctgaag tttgctccaa gtcctcagtt gaaataactg taaatgtttc
     8521 tgtacctgtc attgcaagta ccttcttaaa atatagtgat ttgaaataag atgtgaacaa
     8581 ctctatcagg aaagtcaaat taatttatag aaatatttta gtagccaagg tgt
//
LOCUS       DAVMEF010000077         8628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMEF010000077 DAVMEF010000000
VERSION     DAVMEF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.077"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            200..1129
                     /locus_tag="VYQ30_001288"
     CDS             200..1129
                     /locus_tag="VYQ30_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1966394.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPDKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHP
                     YASWERGTNENHNRLIHRWLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLM
                     ANLNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
     gene            1101..1745
                     /locus_tag="VYQ30_001289"
     CDS             1101..1745
                     /locus_tag="VYQ30_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1966395.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1787..3775)
                     /locus_tag="VYQ30_001290"
     CDS             complement(1787..3775)
                     /locus_tag="VYQ30_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966396.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3777..4535)
                     /locus_tag="VYQ30_001291"
     CDS             complement(3777..4535)
                     /locus_tag="VYQ30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966397.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4963..5154
                     /locus_tag="VYQ30_001292"
     CDS             4963..5154
                     /locus_tag="VYQ30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966398.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5508..5780)
                     /locus_tag="VYQ30_001293"
     CDS             complement(5508..5780)
                     /locus_tag="VYQ30_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1966399.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5839..6288)
                     /locus_tag="VYQ30_001294"
     CDS             complement(5839..6288)
                     /locus_tag="VYQ30_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1966400.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIYHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLT
                     NSHSKPLKLYRCQSCQQCYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            complement(6275..8404)
                     /locus_tag="VYQ30_001295"
     CDS             complement(6275..8404)
                     /locus_tag="VYQ30_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1966401.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIKEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2620 a         1800 c         1670 g         2538 t
ORIGIN      
        1 gattttactg tcctgatcga tttgttctgt tcttgtttca ttttactata tgttcgtaaa
       61 tgaaagttct ataatgaagt tagccaccca gccctcctaa aaattagatg gttcaactag
      121 ccgatagctc ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa
      181 acacaagaaa ggcacagaga tgcaagacaa ttatactaca aaagccaaac atttgacaat
      241 cgatagccgt cgcttaatcg aaagatggaa aaaagaaggg aaatcaaata gagaaattgc
      301 ctctctactt ggaaaagctc ctcaaactat ccacactgaa atcaagcgtg ggacagtccg
      361 acaatgtctt ggaaaagggc gcttcaaaga ggtttattct gccgactatg ctcaacagtc
      421 ttatgaaaac aatcgcaagc gctcggtcaa gaaatcaagc ttgaccaagg aactaaagga
      481 aaagattctc cactatcata accaaaaatt ttcgcctgat aagaaacagg ctagcaccaa
      541 ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa gctatcaatc ttcgcttgga
      601 gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact actgcttgct
      661 tgtcttgacg gatcgaaaga gtagacatca gatcatccga ttgattccaa ataaaagtgc
      721 tgaggtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc tttccatcac
      781 ggcagataat ggaacggaat tcaatcgctt gtctaatgta ttttctgagg agcacatcta
      841 ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca acaggctcat
      901 tcatagatgg ttacctaagg gaaccaagaa aatgactccc aaagaagtcg cattcatcga
      961 aaagtggatt aacaactatc ctaaaaaatg cttgaactac aagtcaccca gagaagactt
     1021 cttgatggct aacttgaact tgaaatttag cgtcagaaac aaatcacgaa atcgattcaa
     1081 attttgcagg tcgtttaaat atcctgcgcg caggtgttct tggtgctaac gatggaatta
     1141 tttccattgc tggtgtggtt atcggggttg ccagtgccac gaccaatatc tggattatct
     1201 ttttatcagg atttgcggct atcttagctg gtgccttttc aatggctggt ggagaatatg
     1261 tatccgtttc aactccaaaa gataccgagg aagctgccgt ttcgcgagaa aaactcttgt
     1321 tagaccaaga tagggaacta gccaaaaaat ccctctatgc tgcttatatc caaaatggag
     1381 aatgcaaaac ttctgcccaa ctcttgacca ataagatctt tcttaaaaat ccactcaagg
     1441 ctctggtaga ggaaaaatat gggattgagt atgaagaatt taccaatcct tggcacgctg
     1501 ccatttctag cttcgttgcc tttttcctta gaagtttgcc tccaatgctg tcagtgacca
     1561 tttttccaag tgattaccgc atccctgcta ccgtccttat tgtcggtgtg gcccttcttc
     1621 tcactggtta cacaagtgct agacttggaa aagccccaac caaaacagct atgattcgga
     1681 accttgctat tggtctcttg accatgggag ttaccttcct gctcgaacaa cttttcagca
     1741 tttagaatac aagaaatacc tcgattttga agtcgaggta tcttttttac atttgcacaa
     1801 tcttgcgata acttcttgaa gtaatcatga aaatcagcac ataggcgatg aggaagatag
     1861 cgcagataga caaggtcaca atcaacatca tagtcgtatc cagtacacca atcactttta
     1921 aaatcaggct aagcatatgg taggcaaagg cgagatgtat gaaggcaaag agcaaaggaa
     1981 ggaagaaaac agttaaaacc tgtttgttga tggtttgctt gatttgcttt tggtccaaac
     2041 cgactttctg caagataata aagcgttcac ggtcttcgta gccttcagaa atttgtttgt
     2101 agtagatgac cagaacagtt ccgaccataa agataatgga taggaaaata ccgataaaga
     2161 agacaccacc aaagagggca ctcatctgag aactagcatc tgctagattg ctaccataaa
     2221 catagctacc ttctgtgtct aattgagcat taaattgatt gaggtagttt tcatactcct
     2281 cagcgacctt gagttgttct gcttcactgg catttacatt cataccaccg taaaactgat
     2341 tatagatagc cgaatctggg aattgatcca aaaaggcttg taaatcagga acaacaaggt
     2401 aattgtaatc agtagtcaag atattaaact tatttggaac atggttgaca atgaaatctt
     2461 tattaaattc ttctttgaca gaaaattgat gatcatttag agttagagct ttctgtcctt
     2521 tcagtccgtc atttttggca aagagaccga cctcatttcc tgatagagac agtttttgac
     2581 cagtcatatt ttcataatct ttttggtcaa ataccatgaa aactgttttt ggttggacac
     2641 ggttttgtcc tttttcaaaa atagttaact tggttccttc ttgatttgca ataccaaagt
     2701 tactgtaacg aagtacttct ttctctttga cactataacc tttgtcactt gcaaactggc
     2761 tcaagagttt gtccaaatct tctttttcaa cattttgccc tgaaacccca aaatcatgag
     2821 gatttagaac ttttttaaag ctttctgcgg aattgaaaat gcttgtcgct gctgacatgg
     2881 ttaccaaaac cattgttgac aaaatagcga tggttgctag tccaactgca tttttcttca
     2941 tacggaaaat caagttggaa acagatatga gattattagg ttggtaatag tatttcttat
     3001 ttttctttaa gatttgaaga aaaacggtaa tccctgcatt gaacaaaaga taagtaccaa
     3061 agataaccag taaaacagct atgaagaagg ttgttaaggc tgtaagagga tcttttaccg
     3121 taagggcaag ataatatcca atccctaaac ttatggaacc aagaatagtt tggagaggaa
     3181 ggaagcgacc ttttttctct ccgctagctt tctcacgtga gagctggagg gcattcatac
     3241 gggcgattcg aagggcattc aggaacatga ggcctaggaa aatcaaaccg aagacaagaa
     3301 gtactgtaat gacaactttc atctggaagg tagcgaccag ttccaccttc aatttcatca
     3361 gtttgagtag gaaagcgaaa atcaacttgt caaacaaggc tccaataccg attcctgctc
     3421 caacagttag aatcccaaat agcactaact ccttaaaggt catactgatt agatggcgct
     3481 tctccaagcc taacatgcca tatataccca gttccttgga acggtttttc atgacaaaac
     3541 tattggcata gaggacgata atagctgacg caagggtaac gacaaacata ccaaatccaa
     3601 gtgttgcttg aatggtggtt cctccacgga tttccgcaat cttgggattg aaggttaggg
     3661 agtaaaagag ataggtgatg gtgactgcca agagaacagc cagtgcaaag ggatagtaga
     3721 gtttgcggtt tttaatcaag ttcgataccg ctaacttatt ggttaatcga aacatactaa
     3781 ttcacctcgc ttgccatgac agtcaaggta tcagagattt cttggaacat ctgacgctct
     3841 gtcttctctc cacggtagat ttgattgtaa agaatgccgt ctttgataaa gagtacacgt
     3901 ttagccctgc tagcagctgc tgttgagtgg gttaccatga ggatggtttg cccacgctca
     3961 ttgatttcat taaagacatc aagtaaggct gcagatgact tggaatcaag ggctcctgtt
     4021 ggctcgtccg caaggagaat ttcaggttct gtgatgatgg cgcgggctac tgctacacgc
     4081 tgtttctgac caccagaaat ctcgtaaggg tacttctctt gcaattggtt gatacccaga
     4141 ttttcagctg tcatcaccaa tttcttcatc atctccgtta taggtcttct tgacaagaca
     4201 agcggaagca agatattgtc cttaacagac agagtatcta gcaagttaaa gtcttggaag
     4261 acaaatccta gcttttcacg ccggaaacta gaagcctgtg aatttttaat agttgctgta
     4321 tcagttccgt ttaggtaaac ctgcccacga cttggtttat ccagcatagc taggatattg
     4381 agcagagtgg atttaccaga accagactca cccatgatgg caacgtagtc acccttttcg
     4441 acggtaaagt gaatatcctt gagggcttct acttggttgc cctgaaaacg tgttttataa
     4501 attttttgaa cgtgttttac atctaaaagt gtcatgagaa tctcctttct taatatccca
     4561 tcaaatgaaa tgtagcttgc atcatagtgc atcccagctg aacgcgctca ctttctttgt
     4621 gacttggttt tctagttttc ttatgtaaga tttgtttcat gatgttactc ctttgttctt
     4681 tatgagtcta gtttacatca aaaaaagacc gcttgccata acctaacctt acaaaagaga
     4741 ctttaatctt acatttttgt aagattgggg gaaatgtaag caaaattact tagaaaatga
     4801 tttgatttcc tatttttaaa attgtaaaat ataagtgtga aaaggcttac tttgaaaagg
     4861 aggaagcggg aactaacatt ttagtaagct tacctagata acataccatt caatttaaca
     4921 cttgcaagtc gttcaaccta taaggaaaga aggtgcgctt taatgaaaca caaagaacat
     4981 atactcatcg gacttctcta tctgctctct ccattcatcg gtcagctctt agttgaacac
     5041 acacacttta tcggtacaga atttaccggc actgcttatg ttatttgctg gttatcagtt
     5101 gttatcagca tccaccattt ttccaaaaat gtattgtctc agcagcaaaa atagattaaa
     5161 aaactgagtt taaattactg cttttataat aaaatgaaat aagaacagga caaatcgatc
     5221 aggatagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagttcc
     5281 aatccactat agtttgtgga tgaagctgac gaaatattat taatactatt caaaaatcaa
     5341 agtgcaaact atgttaactt cacattgccg actaaattac agcctgcggc tggcttccta
     5401 gtttgcactt tgatttttat tgagtataaa agtaaaaaag ctttaaatca agcagtttag
     5461 actattgatt taaagctttt tgcgtattcc aaatcttttg taaaacctca ccaaaaccag
     5521 ccttcattat cgtcaatggc ttgagcttct ttgcgtttac gtgggcctgt tccgtacatt
     5581 tctgcttcga tttcttcctt ggttggcatg atagaagcta ggatgatata gataatcacc
     5641 ccaagtccaa agtttgcgac tgtaaaaatg gcaaacagaa aacgtacaag ggtaacatca
     5701 aagttccact tgtctgacag acctgccaaa actcctgaaa tcatgcgatt tcgtctcatt
     5761 ttataaaatt tactgttcat gatatttcct ctttctgcta ggttagacat agtatatcac
     5821 gggtaggctc ggctttcatc agtcctcagg ctgatttatt agtagcaatt tacctcgaca
     5881 aagtccacag cgatagcgtc tggtatcaat tcttcgcttg cgctgataac attgttggca
     5941 ggattggcaa cgataaagtt tgagtggttt agagtggcta tttgtcaagg atggtacaaa
     6001 ccgtaatcca tccactgctt tcaacagttc cttaaaatcc cgatccttgt gttgatagcc
     6061 tttcccttga aaatagaggt gataatgaca gagttcatgt cggacaattt tcctaaaaac
     6121 gtccaacccc agttcctgat aaaccttggg attaaaatcc aaatgcccat ctttggggaa
     6181 aaatcgccca cctgtcgaac gtagacgcct attccactgg acatgataga taaaaggtct
     6241 gccgaagtct tctagtgaaa cctgcttgac gtaatcagtc agtttcattt ggagctagga
     6301 gagacagatt aactttttca cgttcagtat caattttctt aacccaaacg ctcaccaaat
     6361 ctccaactgc caccacttga ctagggtgtt tgataaactt gcgactcata tgggaaatat
     6421 ggatgagacc gtcctcatga attccgatat caacaaaagc accgaaatca acaacgttac
     6481 gcaccactcc ttctagcttt tgtccaacca ctaggtcttt gatatctagg acatcttgac
     6541 ggagaactgg agcatcaaag gaatcacgga aatctcgacc tggtttgaga agatctgcaa
     6601 tgatatcttt aagagtttct ggaccaaggt ctaactcttg cgccatttcc ttgactgaaa
     6661 gggacttgag tttgctttgg gcttcttcat tcaggtcctt aatgtccaag cgtttgaaga
     6721 gttccttaac ggcagcgtaa ttttctgggt gaactcctgt attatcaaga atattgctac
     6781 tttcagggat acggaggaaa ccagcagcct gttcaaaagc cttggccccc aaacgaggaa
     6841 ctttcttgat ttgggagcgt gaagtgattt ttccctcttc ctcacggtat ttgacgatat
     6901 tttcagaaat ggttttattg agtccagcta cgtgtgaaag aagagctggg ctagctgtat
     6961 tgacattgac accaacttgg ttaaccactg tatcgacaac aaagtccaga ctctcagata
     7021 gtttcttctg actgacatcg tgttggtatt gaccgacacc aattgactta ggatcgattt
     7081 tgaccaattc cgcaagagga tcttgcaaac gacgggcgat agaaatggca gagcgttttt
     7141 caacggtcaa gtctggaaac tcctgacgag caagttcgct ggcagaatag acagaagcac
     7201 cactttcatt aacgataaca tagctgactt cagggaaatc tttcagaact tccgctacaa
     7261 aagcttcact ttcacgactg gccgttccat ttccaatggc aataatctct acaccgtatt
     7321 gaccaattaa atctgctaaa tctttcttgg cttctttgat ttgacgagct gatgctggtt
     7381 taacaggata aataacctga gttgtcagca tttttcctgt tgcatccacg acagctagct
     7441 tggcacctgt acgaaaggct gggtcaaacc caagaaccac gcgccctttc agtggagcaa
     7501 ccaagaggag attgcgcaga ttgtcagaaa aaagttggat agctccctct tcagctttct
     7561 cagttaattc tgtccgaata cgacgctcaa tagcaggcaa gacctttttc ttaacggatt
     7621 gctgaacaac ttcatcaata taagcatttt tcaccttgaa acgagtagca aagaaggcaa
     7681 gaatacggtc cgtcgcatgt tcaaaaccga tcttcaagac accaagtttc tccccacgat
     7741 tgagagccaa ggtacgatag ccttgcatag ttccaactgt ctctgaaaaa tcataataaa
     7801 tctgaaaaac ctgcttttca tcaagacttt catccttggc ttgagaagtg agtttagagt
     7861 gtctcagcac ttcctgataa gtcatagaac gcaaggtcac atcttccgat aaggcttcga
     7921 ccaaaatatc aactgcaccg gtcaaggctt ccttgccagt cgcaaatcct tcacagacga
     7981 acttttcagc ttctttctct aagtcaacta tattctgcaa aatcaagcga gcaagaggaa
     8041 agagtccagc ttcacgggca atggttgcct tggtacgacg cttttcctta taaggaagat
     8101 agagttcttc aacgtctgct aatttttcgg caactaagat agcttcttcc aattccttgg
     8161 tcaacttacc ttgttcttga atcttagcta agacagcttc cttacggtca ttgagatttg
     8221 tcagactttt atccaaatca ataatagcct taatcgccac ctcatccaga ctaccagtca
     8281 tgtccttgcg ataacgcgcg ataaagggaa tagtcgctcc ttcagctgtc aaacttagaa
     8341 cggtatcaat ttgctttaac gtcactccca aatcctgaga gattttttca tattttttat
     8401 ccataaatct attataccac aagctaaacg tttcaaatta actcgtagaa catttaaaaa
     8461 atatgtagga aatagattta tatgctacag cgcaataact tgcacttaaa gagcattgcc
     8521 accttttttt aaccaagcca tgatatcaaa agtatttaat ggatcagaca taatagccag
     8581 ttctggaaga tgttcctgac ctggaataac acattgactt ttcaaatt
//
LOCUS       DAVMEF010000078         8586 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMEF010000078 DAVMEF010000000
VERSION     DAVMEF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.078"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            71..523
                     /locus_tag="VYQ30_001296"
     CDS             71..523
                     /locus_tag="VYQ30_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1966402.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(558..1946)
                     /locus_tag="VYQ30_001297"
     CDS             complement(558..1946)
                     /locus_tag="VYQ30_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1966403.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2012..2974
                     /locus_tag="VYQ30_001298"
     CDS             2012..2974
                     /locus_tag="VYQ30_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1966404.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTYGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3003..3206)
                     /locus_tag="VYQ30_001299"
     CDS             complement(3003..3206)
                     /locus_tag="VYQ30_001299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966405.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(3270..4280)
                     /gene="ccpA"
                     /locus_tag="VYQ30_001300"
     CDS             complement(3270..4280)
                     /gene="ccpA"
                     /locus_tag="VYQ30_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1966406.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4705..5304)
                     /locus_tag="VYQ30_001301"
     CDS             complement(4705..5304)
                     /locus_tag="VYQ30_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966407.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5305..6402)
                     /locus_tag="VYQ30_001302"
     CDS             complement(5305..6402)
                     /locus_tag="VYQ30_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1966408.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(6402..7139)
                     /locus_tag="VYQ30_001303"
     CDS             complement(6402..7139)
                     /locus_tag="VYQ30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966409.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTGFSMMSTAIFSFPAVLHTDKMNNWQKILRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLILGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPVSLFPDWMQAIGKRLPSYQLMELIKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(7141..8025)
                     /locus_tag="VYQ30_001304"
     CDS             complement(7141..8025)
                     /locus_tag="VYQ30_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966410.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(8012..8206)
                     /locus_tag="VYQ30_001305"
     CDS             complement(8012..8206)
                     /locus_tag="VYQ30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966411.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(8203..8334)
                     /locus_tag="VYQ30_001306"
     CDS             complement(8203..8334)
                     /locus_tag="VYQ30_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEV1966412.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKELEE"
BASE COUNT         2583 a         1999 c         1537 g         2467 t
ORIGIN      
        1 actttttttg ctttacttct atagtgaaaa ggtttacaat tatagtaaga aaatttcagg
       61 aggtttcatt atggcacaac gttaccaaaa tatcatggtc gcaatcgatg gttctaagga
      121 agcggacttg gcttttgtca agggagttca ttctgctcta cgaaacgacg ctaaactcac
      181 catcgcacat gtcattgaca cacgcgctct ccaaagcgta tccacctttg atgctgaagt
      241 ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa gagtacgaaa aacgtgctaa
      301 agatgctggg gtagcagatg ttcatatcgt cattgaaatg ggaaatccaa agaccctgct
      361 agcacgtact attccagatg ccgaggaagt ggacctcatc ctcgttggcg caactggtct
      421 caacgccttt gaacgcctct tggtcggctc ttcatctgaa tacatactcc gccatgctaa
      481 ggtcgatttg ctggttgtga gagaacaaga aaaaacctta taatcacaaa gaaaaggagc
      541 ccctagctcc tttttgttta cgatttattt ctctctttat ggcgttcgta agccttgagc
      601 tggcgctgca gttccttttt aatagcaggt tctggagcat atttttcttc ccaattatct
      661 ggttttaaga ttttatgggt cactggatca aaatgagcct tgccatctgg aaaaattttc
      721 cccatattgg cctgatggac aatatcaaaa atacgttctg ggtccacccc catcaagaca
      781 aaactgccgt aggtgaagta aagcgtgtca atcaaggcat ccacttgccc tatcaaatct
      841 tgctgagcag gtgtcttctt ggctacttta cctgctgcct tatcaagggc ctgatgaagt
      901 tgcgatacag cttgaccaaa atcttcttca gaaggactgg ctgatcgaac aaactccacc
      961 aattcttcta ttttaaaacc agccctatgg gttgcaccct ctaaatccca agctcgaggt
     1021 tcttcttggg ttcgttcatc catcatgtgg tggaaggtct tgaccttatt gaaatgatag
     1081 tcacggctga caaaaacttt ttctgaagac aaaacaccaa aatgttccaa ggccttgtgg
     1141 atcccatctt gttgattgct agtggtaatg tgcttggcag cttccttgac actgctacta
     1201 ccatttccca tagcgaccga cataccgacg ccagccagca tttcaaggtc attatctgag
     1261 tcaccaaaag ccatgacttg gttgaggtca aagccatatt ctttcccaac tcggcgaatg
     1321 ccttctaatt tagaatttcc ctgattgatg acatccgatg caaaaggatt gctacgtgtc
     1381 aatttcaagt cttcaaaatc agctgccgcc ttctcagatt cttctggtgt catcagcatc
     1441 aaaacttggt aaataggttg attcatcagg tgaagcaggt cctcttcctt ttggggaaca
     1501 accttgctga ccatacgatt aaaagactga ctcaccgtcc gagttaaaac agagggaacg
     1561 aagcgactaa ttcgttggga aaaagaaccc agaccaaagg acatgatttt agaacccaac
     1621 atggcatcct tggtccctaa ggcaatctcc gtgccctctt ttttagcata gctaattaga
     1681 tggcgcaaat gtaacttgga aatagggctc gtgaacaaga ctctgtcttt actaaagata
     1741 tactggccat tataggttac cgcaaaatcc agatccaaat cgtccatcaa ttccttaaca
     1801 aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc cttgttcttt gacaatctta
     1861 atcgcatcct tagtggattt caaaacactc ttgcgattgt tgaccaaggt tccatcgata
     1921 tcaaaaaaaa cagctttgac ttccatccta tcccaatctc cccttttgtg atacaatgat
     1981 tataccacat ttcagaaaga gtgagtaaat catgcctaag aaaatccttg ttttacatac
     2041 gggtggaact atttccatgc aggccgatgc ttctggcgct gttgtgacga gttcagataa
     2101 tcccatgaac catgtgtcca acccacttga aggaatccaa gtccacgcct tggacttttt
     2161 taaccttcca agtccccata tcaaacccaa acatatgctg gtcctctacc agaaaattaa
     2221 agaggaagca gataactacg atggagtggt gatcacacac ggaaccgata ctttagagga
     2281 aacagcctat ttccttgata ccatggaagt tccccatatg cctatcgttc taacaggagc
     2341 catgcgtagc tccaatgaac tcggtagtga tggtgtttat aattacctaa gtgctttacg
     2401 agtggccagc gatgacaggg ctgctgacaa aggagttttg gtcgttatga acgatgaaat
     2461 ccacgctgcc aagtatgtca ccaaaacaca tacgactaat gtcagcacct tccagactcc
     2521 aacatatggc ccccttggtc tcatcatgaa acaggaaatc ctctacttca aaacagctga
     2581 acctcgtgtt cgctttgacc ttgatcacat acaaggttta gtccctatca tctcggctta
     2641 tgctggtatg acagatgagc tgattgatat gctggattta gaacacttgg acggtttgat
     2701 tatccaagcc ttcggagctg gtaatattcc caaagaaacg gctcaaaaat tagaaagcct
     2761 tctgcaaaaa ggaattccag tcgctctggt atcacgatgc tttaacggta ttgccgagcc
     2821 tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa gcaggcgttt tctttgttaa
     2881 agaactcaac gcccaaaaag ctcgcttgaa actcctcatc gccctcaatg ccggactaac
     2941 aggacaggct ttgaaagact atatggaagg ctaatactct tcgaaaatct ctgcaaacca
     3001 cgtcacgtcg ccttaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca
     3061 aaaacatgtt ttgagctgac ttcgtcagtt ctatctgcaa cctcaaagca gtgctttgag
     3121 ctgacttcgt cagttctatc tacaacctca aaacagtgtg ttgcgcagcc tttaaccagc
     3181 cgcctagtcc gctctatggt attcattaag tcaacatctc ttgtttaaga gcacaaaatc
     3241 aggaaatctt ctcgattccc tgattttttc tatttacgtt ttcgtgttga gctacgttct
     3301 gtcaaaccat gaggtaagag aacttcacgt tcttccaact cttccttatg cataatcttg
     3361 gtcaacatac gcatactaat ggcaccaagg tcataaagag gttgggcaat cgttgtcaag
     3421 tttggacggg taaagcgtga gatttgtgaa tcatcactag taataatttc aaaatcttct
     3481 ggcacagaaa cacccttatc agccaaaccg ttcaagactc ctgctgccaa ctcatcacct
     3541 gtcacaactg ctgcagttgc atttgatgaa atcaaacgct ctgctaaggc gtaaccatca
     3601 tcatagctat atttagattc aaataccaaa ccctcactat aagtgattcc tgcttttttc
     3661 aaggtttcct tgtagccaac taaacgaacc ttaccattga tgtcatccac tagcggaccg
     3721 ctaacgaaag caatacgctc attttcttta gcaaggtaac tcactgcatc aattgttgct
     3781 tgcttatagt caatattgac acttggcaac tggtgctcaa catcgacagt tcctgcgaga
     3841 acaatcggag tacgtgaacg cgaaaattct gagcgaattt tatctgtcaa gtgatacccc
     3901 atatagataa tgccatctac ctgctttgaa aagagggtat tgacaacaga aacttctttc
     3961 tcgttatctt catcgctatt agctaggaca atattgtact tgtacatttc tgcaatatca
     4021 tcaatcccct tagccaaact cgaaaaataa ccattggtaa tatttggaat cacgacaccg
     4081 acagtggttg tttttttact tgcaagacca cgcgcaactg catttggacg ataatccaaa
     4141 cgatcaatta cctctagcac ttttttacgg gtattctctt ttacattttt attgccattg
     4201 accacacggc tgaccgtcgc catggaaaca cctgcttcac gagcgacatc ataaatggtt
     4261 actgtatcat ctgcattcat tccttttcct gtcctttcta tctcacacat tcttttacaa
     4321 gtagaggtac tgattgaagc tctatatcta cttacaaaag tgaagatgtg aaaatttcgt
     4381 tttcatattt ctacttattc cattctatca ctaattgtaa acactttcaa gtgttttttg
     4441 aagattgatt gaaaaaattt catagaaaac ctaggtttag ctccttgcta ccaccttaga
     4501 ctaaacaaaa aggaggaaac taagccctcc taaagttata gtaaaatgaa ataagaacag
     4561 gataaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac
     4621 tattctagtt tcaatctact ataggtattg ttccattcac taccgtcaat tttagcacat
     4681 agtcttcatg aaaatattat atcatcataa ccaaccagat tctttcgcga tattagctgc
     4741 ctctgttcga ttacctgcat ctagtttcga aagaatattg gtgacatagt ttcggactgt
     4801 tccgttggat agataaagtt tgtctgcaat ttcttggtta gagaagccct gagcaattcc
     4861 ctttaaaact gcgatttctt gctccgttaa tggattggga tgcatcatca ccacttccat
     4921 caattcaggc gaatactcct tgcgtccttc gaggacggtg tgcaaggttt gcatgaggtc
     4981 tgcaatgttt ctttctttta atacataagc atctactcca gccttgaccg cacgttcaaa
     5041 atacccagga cgcttgaagg tcgtcaccac aaccaccttt gtttcaagct tttctgctcg
     5101 tatccactcc aagacttcaa gacctgtctt aacaggcatt tctacgtcaa ggatggcgat
     5161 atctacagac tccttttcta atagttggat tgcttcttgc ccattcttgg cttgaaagac
     5221 agactctaca tccggttgaa gcatgagcaa ctggcacatg gcatctcgca acatactttg
     5281 atcttctgcg actaatactt tcatctactt tctctcctta taaagtagtc gaacctgcac
     5341 ttcagttgga tgtttctgac tgattacact tacttctcct gaaaatggaa aaacacgatt
     5401 tcggactgta tggagctcat ccccgcttat agaggcaaag ccacagccat catctctcac
     5461 tgttagaatg agttctttct ctgtccgttc taatttcaag tagactttag acgctttagc
     5521 atgtttgatg atattggtaa ctaattcaag caaaatcatg gaagccgttg actccaattc
     5581 ctgagttaag ctagacttgt ccaagtgatt ctcaacttga acctcaattc cagcaatttc
     5641 taacatcttt ttcacagtct ctagttcgga tgtcaaagtt ctagacttaa gattttccac
     5701 aatggttcgc acttcattca tggaatcctt gctgatctgg tgaatttctt ttaattcctt
     5761 ttccacctgt ggataagcct ccatctgaaa taactgcaag gctaaatctg tcttgacact
     5821 cagcatagca aaggtatgtc ccagactatc atgcaaatcc tgaccgatac gactacgttc
     5881 attttcagca agcaatagat ttatctgagc attttgcttg acctgagctt ctttcaaatc
     5941 ctcgacaata cgaatccgaa ccaatccaaa agtcattaaa tcgacaaaag taagaattac
     6001 aagtagatag aatagaaact caacttcgat tctctgaaaa atcaacagtt gccccacaac
     6061 aaggacttga gcaagaagaa aagtccagac atgtaaagac tttaaactac gtacgctgaa
     6121 atgataactt aagagattgg ataggaaaaa gaaaaaccag atataattaa cagcaacaaa
     6181 ggcagtattc ccaactacat aagtcagcat gaggccccaa tatagccaag ataggcgctg
     6241 gctcttagtt gttaaaacac ccaaatatgc cactacaaat agaatatcaa tcaataaatg
     6301 ccaggcagaa agccacccag tcactacaga caggatgggg aaaatcataa aaattaaact
     6361 gatccaaaac atataatgta ttcttttcag tctttcaagc attaagcatt ctccttatga
     6421 ccttgaaggt aaatagtcaa accaaataaa actgctgaaa aaacaagtag ataaactgtg
     6481 gctgatagat tgattccacc ctcatttaag aaggtcttga ttaattccat caactgataa
     6541 cttggtaaac gcttcccgat tgcttgcatc cagtctggaa ataaagagac gggcatccag
     6601 agtccgccta aaacagccaa tcctagataa agaagattgc ccacgacaga catcagctga
     6661 ctagttggta acagcgtcaa ggttaagcca agtgctacaa aagcaatact tcccaaaatc
     6721 agcaaaatcg cagctccaac ccattttact agaggcatgt ctacacctct tacaaaatgt
     6781 ccaactgtaa aaacaaccag aattgagacc aaataatcaa ccagcatact tgttatcttt
     6841 gatagataat attctaccat atttactggg ctatgacgta atattttctg ccagttgttc
     6901 atcttgtcgg tatgtaagac agctgggaat gaaaagatag cggtcgacat catggaaaag
     6961 ccagtcatgg agatgaggta atcacgcata aaattcgcgg gtccacctgg tgtgtcctgg
     7021 tacatgccag aaaagaatag atagaaggct gtcggcatcc ctactgacaa tagataatag
     7081 atcaattgtc gtttggtcaa taaaaattct atcttactaa gtgctagcca tcgtttcatc
     7141 ttagttatct cccttctgcg tttcttcaaa gattgtatcc aacaaactac gattattaac
     7201 ttcaatttct tgtatgccac atcctgcttg aactaacagt tcccaaaaag catctgcttc
     7261 gcgtgtgact acttgtagag catcctgttt ttgtgaccag ttttcaacca agttagactg
     7321 ctcaatgact tccttgtatg ccagaggaag gataaaatgc ttttcaattc cctcactacg
     7381 catagctaga ggcgtcgtat cacgaatcaa ctctccctta tttaaaacca aaatccggtc
     7441 agccgtatgc tctacctctt caatataatg agacgaatag agaatcgtga ctccttgcgc
     7501 ttttaggtcc cgaacgattt cccaaaagcg ttgacgagtt gaagtatcca tggcagcagt
     7561 tggttcatct aaaaagacaa gctttggtcg cccaatcaag gtcaagacaa aagagaagag
     7621 acgcttttgc ccgcctgaca atttttctgc gaattgctct ttttgttgct ggtcaaactg
     7681 caatagttga tcgatttcct gatcgctcaa ggaatttgga tagatacgtt gaaagaaagc
     7741 aatcaactct ttgaccttta atttctgaac gatgacattt tcttgaggca gataacctct
     7801 aatatagtct aactgagaac tcgtcactga caagccttgg atggatactt gaccgcttgt
     7861 gaccagttta tctccaagca gacagtccaa gagtgtggtc ttcccagcac cattgggccc
     7921 aatcaaggcg acgcattcac cttcagctac ctcaaaggaa atacccttca aaatagcctt
     7981 gcccttgatg tttttattta ggctttctac cttaatcata ttcatgatat tctcctttca
     8041 accactccat tctcataagg aaaacgacga aaatcataaa tccaaacccc aaagcaccac
     8101 gaatgaattg gcgaagcaag gtttggtcaa accaacctgt aaacatttcc actaaccata
     8161 ccaagagtga caggccgata aagaaataga tgatccctct cttcattcct caagctcctt
     8221 tttcacatct ccgactaatt tcaaaccttc tctaacaagc caagacatca ttccaaagcc
     8281 agcaaagagc tcccaaggaa aatgatagaa actctcatcc aatcccgaaa acatgagtta
     8341 ggtcataact cctgctacta ctaaactcac tgcgataatc attttatttc tcatctcttc
     8401 ttcctccatt tcatactaca attatagtct tttgaaatca gaggagacag aagcttctgt
     8461 cactagaaaa tatgacaaat gtcataaaaa attctgttca aaacaagcaa gatacactat
     8521 acaataaaac acaattagaa aaatctaagg caacttcctc aaaagagata tcaaacccaa
     8581 ttcaca
//
LOCUS       DAVMEF010000079         8509 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMEF010000079 DAVMEF010000000
VERSION     DAVMEF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.079"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            360..590
                     /locus_tag="VYQ30_001307"
     CDS             360..590
                     /locus_tag="VYQ30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966413.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEEVEDFQDDDEIPTII"
     gene            673..1476
                     /locus_tag="VYQ30_001308"
     CDS             673..1476
                     /locus_tag="VYQ30_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1966414.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1532..3073)
                     /locus_tag="VYQ30_001309"
     CDS             complement(1532..3073)
                     /locus_tag="VYQ30_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1966415.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3075..3557)
                     /locus_tag="VYQ30_001310"
     CDS             complement(3075..3557)
                     /locus_tag="VYQ30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1966416.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3557..4642)
                     /gene="rlmN"
                     /locus_tag="VYQ30_001311"
     CDS             complement(3557..4642)
                     /gene="rlmN"
                     /locus_tag="VYQ30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1966417.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4663..5193)
                     /locus_tag="VYQ30_001312"
     CDS             complement(4663..5193)
                     /locus_tag="VYQ30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1966418.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(5350..5955)
                     /locus_tag="VYQ30_001313"
     CDS             complement(5350..5955)
                     /locus_tag="VYQ30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1966419.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6128..7165)
                     /locus_tag="VYQ30_001314"
     CDS             complement(6128..7165)
                     /locus_tag="VYQ30_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1966420.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKVDSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7199..8134)
                     /locus_tag="VYQ30_001315"
     CDS             complement(7199..8134)
                     /locus_tag="VYQ30_001315"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1966421.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT         2536 a         1849 c         1602 g         2522 t
ORIGIN      
        1 ttaaaatcat taaaaaacac cccatggtgt gaaccatgaa gtgttgtaaa tgctgtgttg
       61 tagctagttc ttagcgttta gagaactggc tagctttacg agctttctta agatctggtt
      121 tccaaaatac tttgagtaca cttatggtat tgatgcccct aaacatctaa aaactctggt
      181 agaaaaaggg tatgctcttg tcgaaacttc ttttgactca cttgaccatc taaataatac
      241 catgaaaaag aatatcctga agaagaaggg agttactgga ctttctaaaa tgaaatcttg
      301 cgacctggat caagcccttc atgagcattt ttcagaagaa gaattagctg gtcactttca
      361 tgtccttcta tggacttttt tacaatggca ttgctatcac acccaatacc tatctaagcg
      421 cctggttcgt aaactttatt gcagctcttc ctctaaattt cctaattgtt gaaccaattg
      481 cccgttttat actaagttct tttcagaaac catttactgg ggaagaagtt gaagaagttg
      541 aagaagttga agattttcaa gatgatgatg aaatcccaac tattatctaa gccagttctg
      601 taaactacta atatttgaaa tccacttcct tttagggtac aatggtataa atgaattttt
      661 gagaggatca gaatgaaaaa actagcaacc cttcttttac tgtctactgt agccctagct
      721 gggtgtagca gcgtccaacg cagtctgcgt ggtgatgatt atgttgattc cagtcttgct
      781 gctgaagaaa gttccaaagt agctgcccaa tctgccaagg agttaaacga tgctttaaca
      841 aacgaaaacg ccaatttccc acaactatct aaggaagttg ctgaagatga agccgaagtg
      901 attctccaca caagccaagg tgatattcgc attaaactct tccctaaact cgctcctcta
      961 gcggttgaaa atttcctcac tcacgccaaa gaaggctact ataacggtat taccttccac
     1021 cgtgtcatcg atggctttat ggtccaaact ggagatccaa aaggggacgg tacaggtggt
     1081 cagtccatct ggcatgacaa ggataagact aaagacaaag gaactggttt caagaacgag
     1141 attactcctt atttgtataa catccgtggt gctcttgcta tggctaatac tggtcaacca
     1201 aacaccaatg gcagccagtt cttcatcaac caaaactcta cagatacctc ttctaaactc
     1261 cctacaagca agtatccaca gaaaattatc gaagcctaca aagaaggtgg aaaccctagt
     1321 ctagatggca aacacccagt ctttggtcaa gtgattgacg gtatggatgt tgtggataag
     1381 attgctaaag ccgaaaaaga tgaaaaagac aagccaacta ctgctatcac aatcgacagc
     1441 atcgaagtgg tgaaagacta cgattttaaa tcttaaaaac caaaaaaata cagtatccac
     1501 attcggtact gtatttcttt tactctcatt cttaagttaa attattaaaa tcccatattt
     1561 ggtctatcca accttcataa aagtctggct cgtggcagac cataaggata gatcccctgt
     1621 attctttgag agcgcgtttg agctcatcct ttgcatccac atccaaatgg ttggtcggct
     1681 cgtccagcac taaaacgttg ttttcacgat tcatcaagag acagaaacga accttggctt
     1741 gctctccccc tgataatact tgaatctggc tttcaatatg tttggttgtc aaaccacaac
     1801 gggcaagggc tgcacggact tctgcttgat taagggcagg aaaggcattc cagacagctt
     1861 caagaggagt ttggcgatta ccgccttcta cttcctgctc aaaataacca agttctaaat
     1921 aatctccacg ctccacttcc ccagcgattg gcgagataat gcctaagaga ctcttcaaga
     1981 gagttgtttt tccaatacca ttagcaccaa taatcgcaac cttttgattg cgttcgaagg
     2041 taagatttaa aggcttagta agaggacggt cgtaaccaat ttgcaagttc ttggcttgga
     2101 agataaagcg ccctggtgta cgagctggtt tgaaatcaaa ggatggtttt ggtttctcac
     2161 tttggagttc gataatatcc atcttatcca atttcttttg acgagacata gccatattac
     2221 gagttgcaac acgggcttta ttacgagcca caaagtcctt gaggtctgca atctctttct
     2281 gctggcgttc gtaggctgcc tctagctgag atttcttcat agcataaact tcttggaact
     2341 ggtagtagtc accagagtaa cgcgtcagct gttgattttc cacatgatag acaatattaa
     2401 taacgtcatt gaggaatgga atatcgtgcg aaataagaac aaaggcattc tcatagtttt
     2461 ggagatagcg cttgagccaa tcaatatgct cagcatccaa gtagttggtc ggctcgtcca
     2521 acagcaaaat atcaggcttt tcaaggagaa gttttgccaa aagcaccttg gttctttgcc
     2581 cacctgacaa agaagttaca tccgtatcca tcccaaagtc cataacacca agagcacgcg
     2641 ctacttcgtc aatcttagca tccaaggtat agaaatcacg actctccaga cggtcttgaa
     2701 gttctcctac ttcttccatg agagcatcaa catccgcgcc gtcttcagcc attttcatat
     2761 agaggtcatt gatacgagct tcagctttga aaagctcatc aaaagccgta cggagaacat
     2821 cacgcaccga ctgtctttca gcaaggacag agtgctgatc caagtaacca gccgtcacat
     2881 atttggacca ctcaaccttt ccttcatctg gcagcatttt accagtcacg atactcataa
     2941 aggttgattt tccttcacca ttggcaccga ccaggccgat atgttctccc ttgaggagac
     3001 ggaaggacac atcttcaaaa attgcacggt caccaaaacc gtgactcaga tttttaactt
     3061 ctaaaatact cattttaatt ccttatcttg tttttatgta atcgtttata aaggagccaa
     3121 gccagatagc cacccaaagt gttggtccac aaatcatcaa tctcaaagac gcgattgaaa
     3181 tcaaagaaaa agtccaagat taattgcgta cactcgattc caagactcac aagaaaacta
     3241 aaaagaagga ccttttttgt tttccgcaaa tttggaaata gataaaggag ttggaaaatc
     3301 agaggaaaaa acaagaagac attgaggata ttttgtaaaa aaatccaaca taattgtcca
     3361 atgtcactca cttcgcccag tttccagaga gaattgaaag gagtcaaaag aaaaaccagg
     3421 cgtccaagat gctgaatatc tggagttccc actcccacgg tagattgttc ttgaggagta
     3481 aagcaaaaac agacaatgca aatgctatag aaaatgactc cccagaccaa aatatgatta
     3541 taagtcttct tcatcattaa ggatttaccg ctgcgactgc cttctggcgg tcacgtttca
     3601 ttgtgttaga gcgcaattgt ccacaagctg cgtcaatatc tgtaccatgc tcttgacgaa
     3661 ccacacagtt gacccctttt ttcttaagcg tatcatagaa agccaacacg cgctctttgg
     3721 gactacggct atattggtca tgctcactaa ctgggttata aggaatcaag tttacataag
     3781 acaatttctt gatgttcttg agcaattcag ccaattccaa ggcttgttct acaccgtcgt
     3841 tgacttcatt aagcatgata tattcaaagg ttacacgacg gtttgttgtc tcaatgtagt
     3901 attcaatagc agcaaagagt ttttcaatcg gaaaggcacg gttaatcttc atgatacttg
     3961 aacgaagttc attgttgggt gcgtgaagag acacggcaag attgacctga accccttcat
     4021 cagcaaagtc acgaatttta tgagccaaac ctgagattga aaccgtgatg tgacgagcac
     4081 cgatagccat tcctttatca tcattgatag tacgaaagaa attcaagaca ttgttgtaat
     4141 tatcaaaggg ctcaccgatt cccatgacaa cgatatggct gacgcgttca tcctgaccac
     4201 gctcatcaaa gtatttctga accagcatga tttgcgctac gatttcaccg ttattgaggt
     4261 cacgttgctt cttaatcaaa ccagaggcgc agaaggtaca accgatatta cagccgacct
     4321 gagtggtcac acagacagat aaaccatagt gttgacgcat gagtacagtc tcaattaaca
     4381 taccgtcagg caattcaaag agatatttga ctgtaccatc agcagactct tgcacaatac
     4441 gttgtttcaa gggattgacc acaaactggt cattgagctt agcaatcaaa tccttggaaa
     4501 ggttggtcat ttcttcaaat gactgcacac gtttacggta gagccattcc cagatttgat
     4561 ctgcacggaa tttcttttct ccctgctcca atacccattc ctgcatggtt tgatgtacca
     4621 aactatgaat tgagggtttc atttcttctc cttattctct actcacttct gacgaatgac
     4681 aaaatgacgt tgttccttgt cctctttctg agaatgctgg tctttctgac gacgtctatt
     4741 tttcttatct gcattcgact ttcgtttagt ttgagtcggt ttctttcctt ttctagaagg
     4801 tgtttcttct tccgtcttac gcattttctt gtcaaatgat gctcgcttag gggcttcatt
     4861 ttctaagaca aaataggcac aaccataact acaatactct aaaaggtagt cttgtaaacg
     4921 actgattttt tcaagttttt cttctgttcg gtcatccttg taaaaacctc gtaggcgaag
     4981 ctgttcgttg ctccagtccc ccacgatata atcaaacttg gttaagactt ctgaaaaacg
     5041 ctgattaaaa gtcgtcacat caaaggcatc cttgatattt tcaaccaagg aaaaagctat
     5101 cccttccgtt tcgaccttgt ccccgtgtaa atggaactcc ggaccaggaa acttgttata
     5161 gttgtataat tcaggtgcaa tttcttttcg catagatatc ctttttccac gattacttaa
     5221 tactttattc taccataatt tctagcagtt agcacgtttc tcataaaaat gaaaaaagtc
     5281 tgacgatttt gtcagaccag aatcttataa cctaaaaaag agaagaacaa ttcttccctc
     5341 caactatcat tatttagcag ctgcgtacaa ttcatctact ttattccagt tgattactga
     5401 aaagaaagct ttgatgtagt caggacgcac gttgcggtat ttcacgtagt aagcatgttc
     5461 ccaaacgtcc aagcccaaga ttggtttttt accttctgag attggtgtgt cttggtttgc
     5521 tgttgaagtc acttcaagtt tcccttcttt gttgacaacc aaccatgccc aacctgaacc
     5581 aaaacgagtt gttgctgctg cagtgaaggc tgcttggaat tcttcaaatg aaccaaatgt
     5641 tgcatcgatt gctgctgcca gttctgctga aggagctgtt ttctcgggag tcatcaattc
     5701 ccagaaaaga gcgtggttca agtgtcctcc accattgttg ataagtgctt gacggatatc
     5761 agctgggata gattctacat cagcaagcaa ggcttcaagg tcttcaccga tttcagggtg
     5821 tttttctaaa gctgcattgg cattgttgac ataagtttga tggtgtttgt catggtgcaa
     5881 atgcattgtt tccgcatcga tgtatggttc caaagcgtcg tatgcatatg gaagttctgg
     5941 taagataata gccatctgta atacctcttt ttctttctat atgaaaatga taacgcaaac
     6001 attcactttt ttcaagtttt ttgcttatag ataaggaaag cactgacata ctttctaata
     6061 aaataagcaa atcagcaaga aacccacgac aagataatcc tgacgcgggt tgtagtttca
     6121 acaaatgtca attgacctga ctagcaatct gtaagagtgc cttttcaaaa aggaaacctt
     6181 tttcataaag acctgtctta atctgatagt ctgtctcaat caaataggaa atagcttgct
     6241 tcagaaagct caaagaaagt cctctcgaat ctcttaatgc aaacttgatt tgataaggat
     6301 ttgggttacg tcccagataa ctacctaaac tacttgcaat ctgcgattct gtttggccag
     6361 actccgccaa aatcttcacc tgagtaaaag tccgaaattg tcccaacatg accgcaatca
     6421 gtttgatttc atcttcccct tgcaaggtca agtctctcac caaatcgcgc gcctgatcca
     6481 tctttttagt cagaataaac tgagttaaat caaaaatatt gtcctgcaag gtcttgggaa
     6541 ttgcgttaac aatatcctct tcctcgataa cagaatccac cttataggac tgtaaaaaga
     6601 gaagattttt ctggatttcg ctaaattgaa accccgactt gatgaggaga ttttcaaaag
     6661 aatgattggt aaactgcaga ccttgtttct gactccactt ttggaagtac tggcgcaatt
     6721 cttgttcttt tacttctact gcatcgaaga ccttggcatc acgcttaagt aatttgacta
     6781 accgtctttt gctatccagc tttccttctg caaagattat caacttggtt gttggagaag
     6841 gattgtcaag gtattcctca aatgacttaa gctcatcatc tgtcaaaaag cgtttcttag
     6901 cagtcgtgat atccataaaa taatctaata tcacgatttt ctcatccgca aagaaaggaa
     6961 ggctgaccaa ctccagttcc acatccttgt aaactacttc tttcatatca aagtaggcaa
     7021 agttgaggtc agcagaatca tacccaatct gtttcaacac ttgactcttc atcacttcaa
     7081 actgaccctg atctgtccct gtaaataggc tcaggctcgg taaatttgat aaagtcaact
     7141 tctgactttc ttcaatggct agcatcgtct ctcctttctt cagatttttc gatttaattt
     7201 agtcaatata gcgcaatttc ccacggaaat cttctaagct ctcgtagcct ttttccacca
     7261 tgattgcttt cagttcattg gtaatgcggt caaaagcact gacgccttct ttgtgaaggg
     7321 tcgttcccac ctgcaccata cttgctccac agaggatgtg ttcaaaggca tctcgaccag
     7381 tcagaacgcc acctgttccg ataatttgga tttgaggatt taaacgttga taaaaggcgt
     7441 gaacattggc tagagcagtc ggtttgatgt attctccacc aattccacca aaaccattct
     7501 taggccgaat aacgacagat tcgtcttcta tatagaggcc gtttccgata gagttaacgc
     7561 agttgacaaa cttgagcgga tatttgttga aaatagctgc cgcttgatca aagtgaacaa
     7621 tatcaaaata aggtggcaat ttaattccaa gaggtttggt gaagtaagca aacacttctg
     7681 ccaaaatccg gtctgttgtc tcaaaatcat aggcaatctg aggtttacct ggaacatttg
     7741 gacaggaaag atttagctca gtcagaccac gaaaatcact ctcttggact tttttcaaaa
     7801 tagtatgggt ttcctctgga gacatgccga ccagagataa gaagaaagtt cggttcgact
     7861 ctttttcctg caaatctaaa agataatcca aataatagtc taagccatta tttggcaagc
     7921 ccatagagtt gatggaacca agtggaacat cttggtagcg tggctcagga ttcccctgac
     7981 ggaagtccaa ggtcgctgtc ttagtaacaa aggttcctgc cgctgagttt ttgacctctt
     8041 ctaactcctc tatcgtcata caagccacac ctgctgcatt catcaagcaa ttgtcaaact
     8101 caaaaccagc aatttgtgtt ttcgttgata ccatgattct aatcctccag atttctttta
     8161 ttgctaatat tataccataa ttttaattct aggtttacta gattctgtgg ttctatctat
     8221 ttattcggaa aagaaagtct agtacagaag tcatgctatg gaaacagcta gtgtattaga
     8281 agtttgaaca aggcttgggt aacagacttt tgaatgaagt ccaagcaaca aggagaaatc
     8341 tcaaccatga gtccattcca tgactctgtt gctctagaaa accttgcaat agtcaagtga
     8401 taacgaataa aactttggat tgaaatatcc ccatgattat ctattacgag gagaaatctc
     8461 aacctttatc atttttagag aaatatagta aaatgaaaca agaacagga
//
LOCUS       DAVMEF010000080         8452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMEF010000080 DAVMEF010000000
VERSION     DAVMEF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.080"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(74..823)
                     /locus_tag="VYQ30_001316"
     CDS             complement(74..823)
                     /locus_tag="VYQ30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1966422.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            956..3292
                     /locus_tag="VYQ30_001317"
     CDS             956..3292
                     /locus_tag="VYQ30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1966423.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            complement(3729..6875)
                     /locus_tag="VYQ30_001318"
     CDS             complement(3729..6875)
                     /locus_tag="VYQ30_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEV1966424.1"
                     /translation="MIRTEVTSMTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKH
                     PNTYDGISMHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQ
                     ERIGKASHKPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKL
                     QVEQAPRAYVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYF
                     IDRNEHSYFNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFL
                     DDSPFIPEEEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKL
                     ATWIYHYQENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRN
                     EIALNMVWSFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIV
                     DFFLKELPRFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEK
                     AIQALWNQESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSET
                     FKDNEHVNFSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGG
                     ILADDMGLGKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYG
                     SKTERIKQIEKSATITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALR
                     EFDVKTCYALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFVLR
                     RKKDEVLTELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILA
                     GLTRLRQICNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQ
                     ELEKKEMSHFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILC
                     DLWWNPAVEMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQ
                     ESRSNLSVDDIKEILGVE"
     gene            complement(7064..8302)
                     /locus_tag="VYQ30_001319"
     CDS             complement(7064..8302)
                     /locus_tag="VYQ30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966425.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLGLFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
BASE COUNT         2438 a         1603 c         1843 g         2568 t
ORIGIN      
        1 agaggtgtac tattctagtt ccaatctact atataaatga aaaccttagc tgtttggcca
       61 aggttcattt cttctatgca agttcttctc ttttcttatc tgggttccag atgaagcttg
      121 cgataaagct aaagatgatg gttaggatag cgaggataat agcaaggatg agggcaggga
      181 tgcggataaa ccaccagagt ggatttggtt ttttatcatt gtgccattgt ttcgtttctt
      241 cgtccagacg ttggaattct gtttgaatac gattggcgac tgtttcaatg ccttcatcat
      301 tcattttctt gatatctgag atatcgattg gatttccaaa gttcatatcg acacgttcac
      361 ggctaatcaa gcccttcaaa gtcatgggac cggtgtaggt aaccggcatg atacggacct
      421 tggccatttt ggcaatcagt gctgcgcccc ccttgacatc gtttgagtgg cggctaccac
      481 ttggaaacat gatgagagag cggtcacttt ttttgagaac gttgatagga tatttgatgg
      541 ctgaggcgct gggattttca cggtcgatgg gaaaggcgcc acacatacga atccaccaac
      601 caaagatacg gttggtaaag agttcttttt ttgccataaa gatgaactgt tttggcttgg
      661 tcgcaaaggc catataaaca ggatcccacc aggtacggtg aggcgcaact aaaatataat
      721 tttcatcttg attaggaatt ttatcagtat tatgatagtg agcattgcca ttgatggacc
      781 ataagagcaa cacaactaat ccacgcaaat aagtataaaa catgcgatct ccttcgattg
      841 ttttcttgtt attattatac cttatcaaag gagggctggc aaacttttcc cttgactaga
      901 tgcatatttg ggatgaaatt agaattctgt taaaaaaaat gatataatag aatttatgga
      961 taaaaataag attatgggat taacccaaag agaagtcaag gaaagacagg ctgagggttt
     1021 ggtcaatgac tttaccgcat cagccagtac cagcacttgg caaatcgtta aacgaaatgt
     1081 ctttaccctt tttaacgctt tgaactttgc cattgctttg gcccttgcct ttgtgcaggc
     1141 ttggagcaat ctggtcttct ttgctgttat ctgctttaac gctttttctg ggattgtgac
     1201 cgagctacga gccaaacaca tggtggacaa gctcaatctc atgaccaagg aaaaggtcaa
     1261 aaccatccgt gatggtcagg aagttgctct taatcctgaa gaattagtgc taggagatgt
     1321 cattcgtttg tctgcaggag agcagattcc tagtgatgcc ttggttttgg aaggctttgc
     1381 ggaagtcaat gaagccatgt taacgggaga aagtgatttg gtgcaaaagg aagttgacgg
     1441 cttacttttg tcaggaagtt tcctagccag tgggtcagtt ttatctcaag ttcaccatgt
     1501 cggtgcagac aactatgctg ccaaactcat gcttgaggct aagaccgtta aacccatcaa
     1561 ctcccgtatc atgaaatcgc tggacaagtt ggctggtttt actgggaaga ttatcattcc
     1621 ctttggtctg gctctcttgc tggaagcctt gcttttaaaa ggcctgcctc tcaagtcatc
     1681 cgttgtaaac tcgtcgacag ctcttttggg aatgttgcct aagggaattg cccttttgac
     1741 cattacttcg ctcttgactg cagtgattaa gttgggcttg aaaaaggtct tggtgcagga
     1801 gatgtactct gttgagacct tggcgcgcgt ggatatgctc tgtctggaca agacgggcac
     1861 catcacccaa ggaaagatgc aggtggaggc tgttcttcca ctgacggaaa cttacggtga
     1921 agaggctatt gccagcatct tgactagcta catggcccat agtgaggata agaatccaac
     1981 tgcccaagcc attcgccagc gttttgtggg agatgttgct tatcctatga tttccaatct
     2041 tcccttctcg agcgaccgca agtggggggc tatggagtta gaaggcttgg ggacagtttt
     2101 cttaggggca cctgagatgt tgctggattc tgaagtccca gaagccaggg aggccttgga
     2161 gagaggatca cgtgtcttgg tcttagctct cagtcaggag aaattagacc atcacaaacc
     2221 acagaaacca tctgatattc aggctctagc cttgctggaa atcttggacc ccattcgaga
     2281 gggagcagca gagacgctgg actatctccg ttctcaggag gtgggactca agattatctc
     2341 tggtgacaat ccagttacgg tgtccagcat tgcccagaag gctggttttg cggactatca
     2401 cagctatgta gattgctcaa aaatcaccga tgaggaattg atggccatgg cggaggagac
     2461 agctattttc ggacgtgttt cccctcatca aaagaaactc atcatccaaa cgttgaaaaa
     2521 agcgggtcat acaacggcta tgacagggga cggggttaat gatatcttgg cccttcgtga
     2581 ggcggattgt tctatcgtga tggcggaggg ggatccagca acccgtcaga ttgccaatct
     2641 ggttctcttg aactcagact ttaatgatgt tcctgagatt ctcttcgagg gtcgtcgcgt
     2701 ggtcaataac attgcccaca tcgccccgat tttcttgata aagaccatct attccttcct
     2761 gttagcagtc atctgtattg ccagtgcttt actaggtcgg tcagagtgga ttttgatttt
     2821 ccccttcatt ccgatccaga ttaccatgat tgaccagttt gtggaaggtt tcccaccatt
     2881 cgttctgact tttgagcgaa atatcaaacc tgttgagcca aatttcctca gaaaatccat
     2941 gcttcgtgcc ctaccaagtg ccctcatggt cgtgtttagc gttctttttg tgaaaatctt
     3001 tggaacgagt caaggttggt ctgagttaga aatctcaact ctcctctatt atctcttggg
     3061 gtcaattggt ttcttatccg tatttagagc ctgcatgcca ttcactctat ggcgagtcct
     3121 cttgattgtc tggtcagtag gaggcttcct agccacagct ctcttcccaa gaatccaaaa
     3181 actgcttgaa atttcaacct tgacagaaca aacgttgcct gtttatggtg tcatgatgtt
     3241 ggtctttaca gtgattttca tcctgactag tcgctatcaa gctagaaaat aaagaaagac
     3301 tgcaatctgt ggattgcagt tttttatacg atggatctat cgtagatctg atgtgcaagg
     3361 cctacgtgtc tattatctat cggtgaaccc aagagtgatt ctcaagcctg cttggattga
     3421 ggtaatagat tcaaatatct gtagttagac tatttgaagt ctgatgcaag aaataattct
     3481 cttcgaaaat atcttcaaac caggtccgtt ctatccacaa tctcaaagcg gtactttgag
     3541 caacttgtgg tcagctccct agtttgatag tataaaaact gtcagataaa ggggtggcga
     3601 aattgtatct aaacgaacag aaacgtgatg gtaaggcgta tagagctcat aggatggcaa
     3661 ggaaagtcta gaacctaaaa aagaactaga taaatccact aagtgtggtt actctagttc
     3721 tttttcgttt attcaacacc caagatttct ttaatatcat caacgctcag attgcttctg
     3781 ctttcttgtc cttcaaggac ggtgttgaag agttcttttt tactttcttg gagttcttgt
     3841 attttttcct ctatggtacc aagtgtgata aggcgataga catcgacttg tttggtctgg
     3901 ccaaggcggt ggcttcggcc gatggcttgc atctcaactg ctggattcca ccagaggtca
     3961 cagaggatga cggtatcagc tcctgttaga ttcagtcctg ttccacctgc ttttagggaa
     4021 atgaggaagc agtctttttc cccttgattg aaaagattga ccatttcttg acgtttatct
     4081 gaaggtgttt gtcctgttat tttaaagtga gacatttctt ttttctctag ttcttgctca
     4141 atgagatcaa gcatagaggt gaattgtgaa aagataagtg gtcgtgaccc tttttcacga
     4201 atggtgtcta ggagctcgaa taagctgtcc atttttccac tatccccttt gtagtcttct
     4261 aaaaagagag caggggtatt acagatttgt ctaagtcggg tcagtcctgc taagatttca
     4321 attttatgac gtttaatctc ataggcgctg gctcctgaaa ccatttcttg catgcgtcga
     4381 agttgagcta gatagagcgt tttttgcgaa ctgctgagtt cattgctgta gaggtgctcg
     4441 ctcagttcag gcaattctgt cagaacttcg tcttttttcc tacgcagaac aaaaggttga
     4501 atcagtttag caacgagttg aggtgacaat tttgaaaatt cctttttact tggtaggaga
     4561 cctggtaata ctatctggaa aatagaccaa atctcttcga gacggttttc aataggagtt
     4621 ccagataggg catagcaggt tttgacatca aattctctta aagcttgtgc tgttttggtc
     4681 tgactatttt ttatcatctg tgcttcatct aggatgaggt agtcgtaaga ctgcttttga
     4741 taatgttcaa ggtcagatcg aaaagatgga tagcttgtaa tagtgatagt agcagatttt
     4801 tcaatttgtt tgatacgctc agttttgcta ccataggcta cttcaacctg tttgtgaggc
     4861 acaaattttc ggaattcttc cttccaatta taaagaaggc tggctggagc taggataaga
     4921 gctttctggt ctggtttaag gttggcttcc agaagagtga tggtttgcaa ggttttccct
     4981 agacccatgt cgtctgccag gattccccca aaatgataat gatccagcat actcagccac
     5041 ttgactcctt ctttttgata ggagcggagt ttggccttga cttcataagg cttgatatca
     5101 aattcttcag ggtgggtcag gtcataagcc atctttttga aatcacgaga aaaattgaca
     5161 tgttcattgt ctttgaaggt ttcagaaagg ctaaaggcac gagatttgtg catcttgatt
     5221 tttccgtctg aaaatttagc gcgaagatct tggagacttt gagcaacctt gagactttca
     5281 tcgtcaaaga ctagaacctt tccttgcttg gtttggtagt ggctttcttg attccataaa
     5341 gcttggatag ctttttcaac ctcatcttct gaaatcatag aaaaatcaaa ctgaactgaa
     5401 aggaagcttt cgtcgtcaaa aatgtcaatg acagcaggat cttctactaa gagtttttca
     5461 agcgattcat ctagttgaac ttgacccaga gtacgaaaac gggggagttc ttttaggaag
     5521 aaatcaacga ttttgatttt ggaaagacta tgaaattcct ctttgaaacc agcagaaagc
     5581 agttgatgat agatttttct agctttgcta gcttggtaag tataggggag ggttaggaga
     5641 tcttgtttag aatggacttg tttttctcca aaggaccaca ccatattaag ggcaatttca
     5701 tttctagtct tggagaaggc aaaggtgggg ataaagtcat ggatttgtaa ttcatctggg
     5761 atactgatag gaacatagtt tgagatgata ttacagactt gcatgagttc agccttattt
     5821 tcttttgaaa agataagagg agaattttct tgataatgat agatccaagt agctaattta
     5881 tccaaaaagt aacggtgttc ttctttgatg atgtggaagt gattgtgtcg aatccaaagt
     5941 ttttcttgaa taatgaaacc aaaataagct gtattgatga tttcataatg gttattagat
     6001 ttgacaactt taaaaatttc ctcttccggg ataaagggac tatcatctaa gaatataaga
     6061 gggtattcgg cttttgaggt tacatattgg aagacatcca aagcttgaaa aagtgggacg
     6121 aggtcgttta aatagctaac aggagtctct aaatagcgtc cgtaatctct agggttaatg
     6181 ccataactga agttgaaata agaatgttca tttctatcga taaaatattt tttaatatag
     6241 aggagtaggt tttgggatgc ttgattaaac tgattcaagg ataatctgat tgtcccgatt
     6301 ttctgtgaaa caaagtaatc ttccttttgg aaaatgagaa aaatgaagtg agctatatcc
     6361 ttgataacat aggcacgagg agcttgttct acctgtaatt ttaaggtcca ggctaggcta
     6421 tgatttcgga tttcgacttg tccatgaagg gcaagtgaag gcgccgtagc tttttcttct
     6481 accagttcat cttggagctc ttcagatggc aaggtaggaa tcagttggct ggcaaaactt
     6541 ggtttatgac tcgctttacc aatgcgttct tgaatagctt gggggagact acgaaaataa
     6601 agatccatgg caacccagtg tttacagact ccctgatttt ggaaatcctt acattcgcaa
     6661 tgaaagccag tcaaatcgaa gtgctccata ctggtcagga tgttgggctc cagatgcatg
     6721 gaaataccat cataggtatt ggggtgctta aaatattcct tattttcagt atcaaaatag
     6781 ttttttatct gttggaaaat agagcggcca tccattctca tacgagcagg tagtctatcg
     6841 ggaaccgtca ttgaagtaac ctctgttcta atcattcttt tctctatctt atcagaattt
     6901 ataggaaaag aatagattta tcttacataa attgcatctt gatagggatt ggaaacattt
     6961 gtagttgcaa gatagttgta tgtaatatcg acaatcaatt acaaggagat tacgattcca
     7021 tagaaactat caagtgaaaa ctgacttatc cataacattc tagctaaact tcttcttggt
     7081 ttggtaagtt tttcatctgg tgaactagat aggctgctat aagtagtaaa attataagag
     7141 caaaagcaac aacttccaga ccagcacttg ttgcaagaaa tacgagagca agggatagga
     7201 cagctgggat aagtaagttg ataacattga tggatgattg aactgatccc attaactcct
     7261 caggaatgat tccaaaaatc atggcttgca agcgaggact caatactcct gccgagaaag
     7321 catcacccgc acttcctagt aaaataagta aaaaacttcc acgtagaaaa tctataacaa
     7381 tgagagactc catcaataga ctccaataaa tattcaggcg aatagaaatt tttttagata
     7441 gaaatccact cagaagtcca cctaaaataa catgaagaag gcttatcatt attaaaattg
     7501 ccagactttg ccctgttgac aaacccataa aaggatggtg aatcagtaga atcgcactga
     7561 caggctctac catcatcaaa acagcttgac taatggacaa aatccaaagc aagaccatga
     7621 catcttccat agtcatcaat aatttcattg actctttcaa atgctggaaa aagctactca
     7681 ttcctacata aggaggaact tcgatttttt cttcctcttt tttcaagcgg tttcggataa
     7741 gtagacttcc tagaaaggca aataaaaagg ttaaaacatt tacaaaagct agagtagaaa
     7801 tgctaaacag tcctaaaaat actcctccgc tgagattacc tatcaaacgt acaatggaac
     7861 tcgtggactg cctaaaaccc atagcctccg tcatatcatc gtttattagt cgtatgtaaa
     7921 tcggtgtcag catgtatcct gcaagaaaac caatcgtatc tgaaatcaag ttcatgctgc
     7981 aaataaagaa aacagaaaag agagagaagt cggtacttgt caggagcaaa gtcactccag
     8041 aataaaggag gaccttgata aagaggatag aaatatactt gctgattcga tttttttgaa
     8101 aatcagctat aactccagta aaaagttgca agacctgtgg aatggtttct gataggctaa
     8161 tcaaaaatat agctaaggga gcaaaggcat aagaagagac ataattgata aaagctaagt
     8221 aaaaaatagt atctccaaaa cttgaaatcc actcatttac tgttagctga cgaaaagctc
     8281 gatttttaag aaaaatatgc atattaatct cctttactta attcaaatgg gaatgtataa
     8341 ccgagtaaag tccaataaat acccagattt cccacaccaa agtcaaaata actgtttatt
     8401 ccatcttccc cttgaaattg taataatcta tcattattac gataagaacg ag
//
LOCUS       DAVMEF010000081         8322 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMEF010000081 DAVMEF010000000
VERSION     DAVMEF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.081"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            12..203
                     /gene="secG"
                     /locus_tag="VYQ30_001320"
     CDS             12..203
                     /gene="secG"
                     /locus_tag="VYQ30_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1966426.1"
                     /translation="MIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEAVMQRLTG
                     ILVFFWLAIALALTVLSSR"
     gene            305..2659
                     /gene="rnr"
                     /locus_tag="VYQ30_001321"
     CDS             305..2659
                     /gene="rnr"
                     /locus_tag="VYQ30_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1966427.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2622..3089
                     /gene="smpB"
                     /locus_tag="VYQ30_001322"
     CDS             2622..3089
                     /gene="smpB"
                     /locus_tag="VYQ30_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1966428.1"
                     /translation="MVKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3105..3965
                     /gene="tehB"
                     /locus_tag="VYQ30_001323"
     CDS             3105..3965
                     /gene="tehB"
                     /locus_tag="VYQ30_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1966429.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4038..4991
                     /gene="coiA"
                     /locus_tag="VYQ30_001324"
     CDS             4038..4991
                     /gene="coiA"
                     /locus_tag="VYQ30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1966430.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYICPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHIANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5010..6812
                     /gene="pepF"
                     /locus_tag="VYQ30_001325"
     CDS             5010..6812
                     /gene="pepF"
                     /locus_tag="VYQ30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1966431.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTIFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6814..7527
                     /locus_tag="VYQ30_001326"
     CDS             6814..7527
                     /locus_tag="VYQ30_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1966432.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7594..>8322
                     /gene="prsA"
                     /locus_tag="VYQ30_001327"
     CDS             7594..>8322
                     /gene="prsA"
                     /locus_tag="VYQ30_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1966433.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKT"
BASE COUNT         2655 a         1536 c         1864 g         2267 t
ORIGIN      
        1 tattatctgt tgtgattgtg attgcaattt tcatgcaacc aaccaaaaac caatccagca
       61 atgtatttga tgccagttca ggtgatttgt ttgaacgcag taaagctcgc ggttttgaag
      121 ctgtaatgca gcgtttgaca gggattttag tctttttctg gctagccatt gccttagcat
      181 tgacggtatt atcaagtaga taagaaaata atgggcagga ctaggtcttt gcctcttttt
      241 atttttaaag gatgtttgag aaggttttac agtaaaagaa aattaaaaaa tctagaaaga
      301 aaatatgaaa gatagaataa aagaatattt acaagacaag ggaaaggtga ctgttaatga
      361 tttggctcag gctttgggaa aagacagttc caaggatttt cgtgagttga ttaaaacctt
      421 gtccttaatg gaaagaaagc accaaattcg ttttgaagaa gatggtagtc tgacattaga
      481 aattaagaaa aaacatgaga ttaccctcaa ggggattttt catgcccata aaaatggctt
      541 tggctttgtt agtctggaag gcgaggagga cgaccttttt gtagggaaaa atgatgtcaa
      601 ctatgctatt gatggtgata ccgtcgaggt agtgattaag aaagtcgctg accgcaataa
      661 gggaacagca gcagaagcca aaattattga tatcctagaa cacagtttga caacagttgt
      721 cgggcaaatc gttctggatc aggaaaagcc taagtatgct ggctatattc gttcaaaaaa
      781 tcagaaaatc agtcaaccga tttatgttaa gaaaccagcc ctaaagttag aaggaacaga
      841 agttctcaag gtctttatcg ataaataccc aagcaagaaa catgatttct ttgtcgcgag
      901 tgttctcgat gtggtgggac actcaacgga tgtcggaatt gatgttcttg aggtcttgga
      961 atcaatggac attgtatccg agtttccaga agctgttgtc aaggaagcag aaagtgtgcc
     1021 tgatgctccg tctcaaaagg atatggaagg tcgtctggat ctaagagatg aaattacctt
     1081 taccattgac ggtgcggatg ccaaggactt ggacgatgca gtgcatatca aggctctgaa
     1141 aaatggcaat ctggagcttg gggttcacat cgcagatgtt tcttattatg tgaccgaggg
     1201 gtctgccctt gacaaggaag cccttaaccg tgcgacttct gtttacgtga cagaccgagt
     1261 ggtgccaatg cttccagaac gactatcaaa tggcatctgc tctctcaatc cccaagttga
     1321 ccgcctgacc cagtctgcta ttatggagat tgataaacat ggtcgtgtgg tcaactatac
     1381 cattacacaa acagttatca agaccagttt tcgtatgacc tatagcgatg tcaatgatat
     1441 cctagctggc gatgaagaaa agagaaaaga atatcataaa attgtatcaa gtatcgaact
     1501 catggccaag cttcatgaaa ctttagaaaa catgcgtgtg aaacgtggag ctctcaattt
     1561 tgataccaat gaagcgaaga ttttagtgga taaacaaggt aagcctgttg atatcgttct
     1621 tcggcagcgt ggcattgccg agcggatgat tgagtctttt atgttgatgg ctaatgaaac
     1681 agttgccgaa catttcagca agttggattt gccttttatc tatcgaattc acgaggagcc
     1741 taaggctgaa aaggttcaga agtttattga ttatgcttcg agttttggct tgcgcattta
     1801 tgggactgcc agtgagatta gtcaggaggc acttcaagac atcatgcgtg ctgttgaggg
     1861 agaaccttat gcagatgtat tgtccatgat gcttcttcgc tctatgcagc aggctcgtta
     1921 ttcggagcac aatcacggcc actatggact agctgctgac tattatactc actttaccag
     1981 tccaattcgt cgttatccag accttcttgt tcaccgtatg attcgggatt acggccgttc
     2041 taaggaaata gcagagcatt ttgaacaagt gattccagag attgcgaccc agtcttccaa
     2101 ccgtgaacgt cgtgccatag aagctgagcg tgaagtcgaa gccatgaaaa aggctgagta
     2161 tatggaagaa tacgtgggtg aagagtatga tgcagttgta tcaagtattg tcaaattcgg
     2221 tctctttgtc gaattgccaa acacagttga aggcttgatt cacatcacta atctacctga
     2281 attttatcat ttcaatgagc gtgatttgac tcttcgtgga gaaaaatcag gtatcacttt
     2341 ccgagtgggt cagcagatcc gtatccgtgt tgaaagagcg gataaaatga ctggagagat
     2401 tgatttttca ttcgtaccta gtgagtttga tgtgattgaa aaaggcttga aacagtctag
     2461 tcgtagtggc agagggcgtg gttcaaatcg tcgttcggat aagaaggaag acaagagaaa
     2521 atcaggacgc tcaaatgata agcgtaagca ttcacaaaaa gacaagaaga aaaaaggaaa
     2581 gaaacctttt tacaaggaag tagctaagaa aggagccaag catggtaaag ggcgagggaa
     2641 aggtcgtcgc acaaaataaa aaggcacgcc acgactatac aatcgtagat acgctagagg
     2701 cagggatggt cctgactgga actgaaatca agagtgtacg agctgctcga attaatctca
     2761 aggatggctt tgctcaagtg aaaaatggag aagtttggct gagcaatgtt catatcgcgc
     2821 cttacgaaga gggcaatatc tggaaccagg aaccagaacg tcgtcgtaaa ctcctgcttc
     2881 ataaaaagca aattcaaaaa ttggaacaag agaccaaagg gacaggaatg accttagttc
     2941 cccttaaggt ctatataaaa gatggctacg ctaagcttct tttaggactt gccaaaggga
     3001 agcatgacta tgacaaacgg gagtctatca aacgtcgtga gcaaaatcga gatatcgcgc
     3061 gtgtgatgaa agctgttaat cagcgataaa aagaggaatt gaaaatggaa aaattagttg
     3121 cctataaacg catgcctttg tggaataaac aaacaatgcc tgaagctgtt cagcaaaagc
     3181 acaatacaaa agttgggact tgggggaaaa ttactgtctt gaagggagct ctcaagttta
     3241 ttgaattgac agaagaaggg gaagttctag ctgaacacct ctttgaagca ggggcagaca
     3301 atccaatggc ccaacctcaa gcctggcacc gagtggaagc tgccacagat gatgtggaat
     3361 ggtacttgga attttattgt aaacctgagg attattttgc taaaaaatac aataccaatc
     3421 ctgttcattc agaggtccta gaggccatgc agacagtgaa acaagggaaa gctttggatt
     3481 tgggttgtgg tcaggggcgt aattctcttt ttctagccca gcaagatttt gatgtgacgg
     3541 ctgtagatca aaatggacta gctcttgaaa tcttgcaaag cattgtggag caggaagatt
     3601 tggacatgcc tgttggcctt tacgatatca attcagctag cattgaacaa gaatatgatt
     3661 ttatcgtttc aacagttgtt ctcatgtttc tacaagcgga ccgcattcca gctattattc
     3721 aaaatatgca ggagaaaacc agtgttggtg gttacaacct tatcgtttgt gccatggaca
     3781 cggaggatta tccttgctcg gttaacttcc cattcacctt taaagaagga gaactggcag
     3841 actattacaa ggattgggaa ttggttaagt acaatgaaaa tccaggccat ttgcaccgtc
     3901 gcgatgagaa tggcaatcgt attcaactac gctttgcgac cttactagct aagaaaatca
     3961 agtaaacaca catgaagatt aggaattttc ctgatctttt ttctttttta cgaatgatat
     4021 agaaaaggag ggaattcatg tttgttgcga gagatgctag gggagaattg gtaaatgtgt
     4081 tagaggataa acttgagaag caagcataca tctgcccagc ttgtggaggc cagatccatt
     4141 tgcgtcaagg accaagtgta cggacgcatt ttgcccataa atccttaaaa gactgtgatt
     4201 ttttctttga aaatgaaagt ccagaacaca tagccaataa ggaatccctc tatcactggt
     4261 tgaaaaaaga gacaaaggtt caattagagt acccgctttc agaacttaaa cagattgcgg
     4321 atgtatttgt aaatggcaat ctagctctag aagttcagtg tagtcccttg cctcagaaag
     4381 tccttaaaga gcgaagtgag ggctatcgta gtcagggtta ccaagtactg tggttgctgg
     4441 gtcaaaaact gtggctcaag gagcgtttga ctcgtctaca gcaaggtttt ctttatttca
     4501 gtcaaaacat gggcttttat gtttgggaat tagacaagga aaaacaagtt ttaagactca
     4561 aatacctgat ttaccaggat ctccgcggta aactccatta tcaaatcaag gaattttcct
     4621 atggtcaagg tagtttattg gaaatattgc gtcttcccta taagagacaa aaaatatctc
     4681 attttacagt ttctgaggac aaggacatct gtcgctatat ccggcaacaa ctttattatc
     4741 aaaatctctt ttggatgaaa gaacaagcag aagcctatca aaagggagaa aatatcctga
     4801 cttatggact gaaagaatgg tatccacaaa ttcgaccaat agtgggcaaa tttttccaga
     4861 ttgaacaaga cttgactagc tattatcagc acttttatac ctattaccaa aaaaatcctc
     4921 aaaatgattg gcaaaagctt tatccaccag ccttttatca gcaatatttc ttgaaaaata
     4981 tggtagaata gaaaggatgg aggaatctaa tggtattaca aagaaatgaa ataaatgaaa
     5041 aagatacatg ggatctatca acgatctacc caactgacca ggcttgggaa gaagccttaa
     5101 aagatttaac agaacaattg gagacagtag cccagtatga aggccatctc ttggatagtg
     5161 cggataacct actagaaatc actgaatttt ctcttgaaat ggaacgccag atagagaagc
     5221 tttacgttta tgctcatatg aagaatgacc aggatacacg tgaagctaag tatcaagagt
     5281 actatgccaa ggccatgaca ctctacagcc agttagacca agccttttca ttctatgagc
     5341 ctgaatttat ggagattagc gaaaagcagt atgctgactt tttagaagct caaccaaagc
     5401 tgcaggttta tcaacactat tttgacaagc tcttgcaagg caaggatcac gttctttcac
     5461 aacgtgaaga agaattattg gctggagctg gagaaatctt tggttcagca agtgaaacct
     5521 tcgctatctt ggacaatgcg gatattgtgt tcccttatgt cctagacgat gatggtaaag
     5581 aagttcagct atctcatggg acttacacac gtttgatgga gtctaaaaaa cgtgaggttc
     5641 gccgtggtgc ctatcaagct ctttatgcga cttacgaaca attccaacac acctatgcca
     5701 aaaccttgca aaccaatgtt aaggtgcaaa attaccgtgc taaagttcgt aactacaaga
     5761 gtgctcgtca tgcagccctc gcagcgaatt ttgttccaga aagtgtttat gacaatttgg
     5821 tagcagcagt tcgcaagcat ttgccactct tacatcgcta tcttgagctt cgttcaaaaa
     5881 tcttggggat ttcagatctc aagatgtacg atgtctacac accgctttca tctgttgaat
     5941 acagttttac ctaccaagaa gccttgaaaa aagcagaaga tgctttggca gtcttgggtg
     6001 aggattactt gagccgtgtt aaacgtgcct tcagcgagcg ttggattgat gtttacgaaa
     6061 atcaaggcaa gcgttcaggt gcctactctg gtggttctta cgataccaat gcctttatgc
     6121 ttctcaactg gcaggacaat ctggacaatc tctttactct tgttcatgaa acaggtcaca
     6181 gtatgcattc aagctatact cgtgaaactc agccttatgt ttacggagat tactctatct
     6241 ttttggctga gattgcctca actaccaatg aaaatatctt gacggagaaa ttattggaag
     6301 aagtggaaga cgacgcaaca cgctttgcta ttctcaataa cttcctagat ggtttccgtg
     6361 gaacaatttt ccgccaaact caatttgctg agtttgaaca cgccattcac caagcagatc
     6421 aaaatgggga ggtcttgaca agcgatttcc taaataaact ctacgcagac ttgaaccaag
     6481 agtattatgg tttgagtaag gaagacaatc ctgaaatcca atacgagtgg gctcgcattc
     6541 cacacttcta ctataactac tatgtatatc aatattcaac tggctttgcg gccgcctcag
     6601 ccttggctga aaaaattgtc catggtagtc aagaagaccg tgaccgctat atcgactacc
     6661 tcaaggcagg taagtcggac tatccactta atgtcatgag aaaagctggt gttgatatgg
     6721 agaaggaaga ctacctcaac gatgcctttg cagtctttga acgccgttta aatgagtttg
     6781 aagcccttgt tgaaaaatta ggattggcat aaaatggttg aatcgtatag taagaatgct
     6841 aaccataaca tgcgtcgtcc tgtcgtcaaa gaagaaattg tagacttgat gcgtcagcgt
     6901 caaaagcagg tcacaggttt cttgaaagaa ttggaagact ttgcccgcaa ggaaaatatt
     6961 cctattattc cccatgaaac ggttgcttat ttccgttttc ttatggaaac catgcagcct
     7021 aaaaatattc tggaaattgg gacggctatc ggtttttcag ctctcttgat ggctgaacat
     7081 gcgccaaatg ctaagattac aactattgat cgtaatccag aaatgattgg ttttgccaag
     7141 gaaaattttg cccagtttga cagtcgcaag caaatcactc tcctagaggg agatgcggtg
     7201 gatgtcttat ctacactgac agagtcttat gatttcgtat ttatggattc tgccaagtct
     7261 aaatacatcg tctttctgcc agaaatcctc aaacatttgg aagttggtgg tgtggttgtc
     7321 ttggatgata tttttcaagg tggtgatgtt gccaaggata ttatggaagt ccgtcgtggt
     7381 cagcgaacca tttatcgagg ccttcaaaaa ttatttgatg caaccttaga caatccagaa
     7441 ctcaccgcaa cattagtgcc tttaggagat ggtattctca tgcttcgtaa aaatgtagca
     7501 gatgttcaac tgtctgaaag cgaatgattt tcagaaaaat ttaagaaaaa atagtaaaat
     7561 agatagagta acacttatct caaaggagta gacatgaaga aaaaattatt ggcaggtgcc
     7621 atcacactat tatcagtagc aactttagca gcttgttcga aagggtcaga aggagcagac
     7681 cttatcagca tgaaagggga tgtcatcaca gaacatcaat tttatgagca agtgaaaaac
     7741 aacccttcag cccaacaagt cttgttaaat atgaccatcc aaaaagtttt tgaaaaacaa
     7801 tatggctcag agcttgatga taaagaggtt gatgatacta ttgccgaaga aaaaaaacaa
     7861 tatggcgaaa actaccaacg tgtcttgtca caagcaggta tgactcttga aacacgtaaa
     7921 gctcaaattc gtacaagtaa attagttgag ttggcagtta agaaggtagc agaagctgaa
     7981 ttgacagatg aagcctataa gaaagccttt gatgagtaca ctccagatgt aacggctcaa
     8041 atcatccgtc ttaataatga agataaggcc aaagaagttc tcgaaaaagc caaggcagaa
     8101 ggtgctgatt ttgctcaatt agccaaagat aattcaactg atgaaaaaac aaaagaaaat
     8161 ggtggagaaa ttacctttga ttctgcttca acagaagtac ctgagcaagt caaaaaagcc
     8221 gctttcgctt tagatgtgga tggtgtttct gatgtgatta cagcaactgg cacacaagcc
     8281 tacagtagcc aatattacat tgtaaaactc actaagaaaa ca
//
LOCUS       DAVMEF010000082         8276 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMEF010000082 DAVMEF010000000
VERSION     DAVMEF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.082"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(841..1110)
                     /gene="rpsO"
                     /locus_tag="VYQ30_001328"
     CDS             complement(841..1110)
                     /gene="rpsO"
                     /locus_tag="VYQ30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1966434.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1251..1880)
                     /locus_tag="VYQ30_001329"
     CDS             complement(1251..1880)
                     /locus_tag="VYQ30_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1966435.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2750..3073
                     /locus_tag="VYQ30_001330"
     CDS             2750..3073
                     /locus_tag="VYQ30_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1966436.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3131..5074
                     /gene="thrS"
                     /locus_tag="VYQ30_001331"
     CDS             3131..5074
                     /gene="thrS"
                     /locus_tag="VYQ30_001331"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1966437.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELVSGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5123..6097)
                     /locus_tag="VYQ30_001332"
     CDS             complement(5123..6097)
                     /locus_tag="VYQ30_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1966438.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6090..6767)
                     /locus_tag="VYQ30_001333"
     CDS             complement(6090..6767)
                     /locus_tag="VYQ30_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966439.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            6923..7996
                     /locus_tag="VYQ30_001334"
     CDS             6923..7996
                     /locus_tag="VYQ30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1966440.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            complement(8087..8276)
                     /locus_tag="VYQ30_001335"
                     /pseudo
     CDS             complement(8087..8276)
                     /locus_tag="VYQ30_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2530 a         1801 c         1536 g         2409 t
ORIGIN      
        1 atagcagata tgattgtctg tcccataaaa aaacctcctg tattagtcag taataaatca
       61 gcagatttta ggagagcaca gacacattaa tttagctatc gctagtgggt aatatcgaac
      121 gtaggaaagc attttactcc tcctcaaaac gtagttacaa agtaatttct aactggaatt
      181 cagtgactac ttccatgtaa agtataacat actatacttt actttgtaaa gagtgggctc
      241 ttctaggggg cttgcataca gctactcggc ttgaaaagcc gagtaactgc gcacctttat
      301 ggtgtaatta gctgataccg tttgaggttt ttgtagtctc caaaattgtt accgataaaa
      361 cctcaataaa aaagccctct gaagtcagag agctgatttg agcccgggct aaaatcctag
      421 tgaaaaagat gaaactcctt gtgctcatcg aacactgtgt catttcccta ttttcatacg
      481 gatttttgac gcccttagca tcatgattct tgctagataa atcgtttata gactaggcga
      541 cgaggcgaag atcgtacgaa actttcagtg aaacaaaaaa aatctccccg aagggagaaa
      601 agctggttta ttttacctta cagtgctagg aaccgtaacc aaagattata cttgagtata
      661 tcgaggtaag gtgacaacgc aaaaaagcta cccaatttag agtagcttta ttatcaagat
      721 atgctcaatt gaacacggcc taagcacttg gcaaaaaaga taaaatctcc tagaaactga
      781 agtttcttcg tcaattttcc tatttttgct ttgtgctttt gacggccttt gtatcttgaa
      841 ttagcgacga agtcctagag agttgatcaa ctcacggtaa cggttaacgt cgtttttacg
      901 caagtatgca agcaagttac gacggcgacc gatttttttc atcaatccac ggtaagtagc
      961 gtggtctttt ttgtgttgtt tgatgtgttc gttaaggtgg ttgatttccc aagtaaggac
     1021 agcaacttga acctctactg aacctgtatc accttcgtga cgtgcatatt gtgcgatgat
     1081 ttcatttttt ttctcttttg agattgccat gatgtttctc ctttttattt ggcttcatcc
     1141 gagtgacagg ttggcatgcc tgtaaccaag aagaagttat ttgtctttac gacaatccct
     1201 attttaccaa gatttaaatt ttctgtcaaa cattaatact ctcatagtta ttatttttca
     1261 agtaaagaga tacgttcttt tatatgatga cctttttcaa gttcatctac caatccaata
     1321 gcataatctg cataactaat ctggcttatt cctttttcat tagtagtaaa aatttctcct
     1381 gctagaatgt agtttcctgt tttttcacca tcgggaataa aatctacagc aggactgaca
     1441 aaagtccagt tgagattatt ttttgtacga agaagatcca attcatccgc ttgagctttt
     1501 gctagtggct taaattcttc aggaaaatca ggcgtatcta aaagtcgtgt cgtttttgtt
     1561 tcatctatat aaagacttcc tgctccccct acaactagaa aacgtgtctg tgttccagca
     1621 agaagctgat taaatagttc gattgatttg ctgtggagcg gtagcgtatc tggtgtataa
     1681 gcaccaaacg ctgaaataac agcatcaaat ccagtaagat catcttttgt caactcaaat
     1741 aaatcttttt taataataga ctcagcttga cttttgtttt cagaacgaac aatagccgtt
     1801 acttcatgtc ctcgtttgac tgcttcttca acaattgctt tccccgcttg tccatttgct
     1861 gcaataactg ctagtttcat tttttatacc tctcttgttg taattatttt agttacaaca
     1921 attgtgacac tcttaataat caatgtcaat agtcttgctt aattattatc aaaatatttc
     1981 taccaagaaa actaaccatg attctagtga aaagaaaaat cttctttgtc aacaaattta
     2041 ctttcttgtt ttaaacatgc tataataatc atagcaagag atctaagttg tctgtttttt
     2101 taaaacgagg tgattatcat gcgtagattc tattcccatc tcccctacta tctggtcata
     2161 ttattctttt attggccact ttatgagttg ttcttactag ttgtttctga cccccttaca
     2221 ctcaaggaac tctatataaa caatcttctc ttctttacac ctctggtaat cttgattata
     2281 tcgttactct atagctaccg tttccgtttc tcactttgat ggttagttgg taacggactg
     2341 ctcttttact ttactatcat aacctttggt gagtttatac taatttactt gctaatctat
     2401 gaaacagttg ctctggtcgg catggattct ggtattagca tcaagcatat tctacaaaaa
     2461 atgaaaaaca aaaaactttc acaaaatcct tgaaaaatct cacaatcatg ctataataat
     2521 ccatagagac aagtcactta gtccctttct actagagagt gcgtggttgc tggaaacgca
     2581 taggaagtct aaactgatac tactcttgag ttttttatga aaacataaaa cggtggccac
     2641 gttagagccg atcagaggtg tccctctctt ttgaggtaca taaatgaagg tggaaccacg
     2701 ttgcgacgtc ctttcgagga tgtcgcattt ttttattagg atactaatta tggagttgca
     2761 agaattagtg gagcgcagtt gggcaatccg acaagcttat cacgaactgg aagttaagca
     2821 tcatgattcc aagtggacgg tagaagaaga cctcttggct ttatctaatg atattggaaa
     2881 tttccaacga ctggtgatga caaagcaagg acgctactat gatgaaacac cctacacact
     2941 ggaacaaaaa ctttcagaaa atatctggtg gctattagaa ctttctcaac gtttggatat
     3001 agacattctg acggaaatgg aaaacttcct ctctgataaa gaaaagcaat tgaacgttag
     3061 gacttggaag tagtctgctg ataaaaaatc aatgcttaga aactatgaaa taataaaaaa
     3121 ggagaacatc atgattaaca ttactttccc agatggcgct gttcgtgaat tcgaatctgg
     3181 cgtaacaact tttgaaattg cccaatctat cagcaattcc ctagctaaaa aagccttggc
     3241 tggtaaattc aacggcaaac tcatcgacac tactcgcgct atcactgaag atggaagcat
     3301 cgaaattgtg acacctgatc acgaagatgc ccttccaatc ttgcgtcact cagcagctca
     3361 cttgttcgcc caagcagctc gtcgtctttt cccagacatt cacttgggag ttggtccagc
     3421 catcgaagat ggtttctact acgatactga caacacagct ggtcaaatct ctaacgaaga
     3481 ccttcctcgt atcgaagaag aaatgcaaaa aatcgtcaaa gaaaacttcc catctattcg
     3541 tgaagaagtg actaaagacg aggcacgtga aatcttcaaa aatgaccctt acaagttgga
     3601 attgattgaa gaacactcag aagacgaagg cggtttgact atctatcgtc agggtgaata
     3661 tgtagacctc tgccgtggac ctcacgttcc atcaacaggt cgtatccaaa tcttccacct
     3721 tctccatgta gctggtgcgt actggcgtgg aaacagcgac aacgctatga tgcaacgtat
     3781 ctacggtaca gcttggtttg acaagaaaga cttgaaaaac taccttcaaa tgcgtgaaga
     3841 agctaaggaa cgtgaccacc gtaaacttgg taaagagctt gacctcttta tgatttcaca
     3901 agaagtggga caaggtttgc cattctggtt gccaaatggt gcgactatcc gtcgtgaatt
     3961 ggaacgctac atcgtaaaca aagagttggt ttctggctac caacacgtct acactccacc
     4021 acttgcttct gttgagcttt acaagacttc tggtcactgg gatcattacc aagaagacat
     4081 gttcccaacc atggacatgg gtgacgggga agaatttgtc cttcgtccaa tgaactgtcc
     4141 gcaccacatc caagttttca aacaccatgt tcactcttac cgtgaattgc caatccgtat
     4201 cgctgaaatc ggtatgatgc accgttacga aaaatctggt gccctcactg gccttcaacg
     4261 tgtacgtgaa atgtcactca acgacggtca cctattcgtt actccagaac aaatccaaga
     4321 agaattccaa cgtgcccttc agttgattat cgatgtttat gaagacttca acttgactga
     4381 ctaccgcttc cgcctctctc ttcgtgaccc tcaagatact cataagtact ttgataacga
     4441 tgagatgtgg gaaaatgccc aaaccatgct tcgtgcagct cttgatgaaa tgggcgtgga
     4501 ctactttgaa gccgaaggtg aagcagcctt ctacggacca aaattggata tccagattaa
     4561 aactgccctt ggaaaagaag aaaccctttc tactatccaa cttgatttct tgttgccaga
     4621 acgcttcgac ctcaaataca tcggagctga tggcgaagat caccgtccag tcatgatcca
     4681 ccgtggggtt atctcaacta tggaacgctt cacagctatc ttgattgaga actacaaggg
     4741 ggccttccca acatggctgg caccacacca agtaaccctc atcccagtat ctaacgaaaa
     4801 acacgtggac tacgcttggg aagtggccaa gaaactccgt gaccgcggtg tccgtgcaga
     4861 cgtagatgag cgcaatgaaa aaatgcagtt caagatccgt gcttcacaaa ccagcaagat
     4921 tccttaccaa ttaattgttg gagacaaaga aatggaagac gaaacagtca acgttcgtcg
     4981 ctacggccaa aaagaaacac aaactgtctc agttgataat tttgttcaag ctatcctagc
     5041 tgatatcgcc aacaaatcac gcgttgagaa ataagagtct agcataaaag cctccaatct
     5101 ggaggctttt tctcatctat ttttactcaa ggactaagtt cacttgagca aactgaatcc
     5161 gcactgtcgt tccttttccg acctcagact cgatacgaat ctggtgcccc agttcttcag
     5221 aaattttctt agatagataa aggccaagtc cagaggactg ctgggtcaaa cggccattgt
     5281 atcctgaaaa gccacgttca aatactcgga ggacatcact gttttttatc ccgattcccg
     5341 tatctttgat acaaagctct tggtcatcca tataaatctc cagaccacct tccttggtgt
     5401 acttgagact gtttgagatg atttgctcaa taaccactag cagccacttt ttatccgtca
     5461 cgatttcttt atcaaggtca tgtagattga catttaagcc tttttgaata aagaaaagag
     5521 catatttacg aattatttcc ttgaccaagt cctcaatttg aacctgcttt aagaccaaat
     5581 catcatggaa actttctaaa cgcaggtact gtaaaactag gttggtatag gagtcgattt
     5641 tgaaaatttc ctgttctagc tgctgcttca gttggcggtc gaccacttct gcaactaaga
     5701 gttgactggc tgcaatgggg gtctttatct gatggaccca caaggtatag taatccagca
     5761 aatccgtcag ttttctttct gcttttgacc tctgctgata gagttccatc tcacgcgctt
     5821 ctaatttttc tgctaaagct atttccaaag gagacttggc ttccctctct ccatagagaa
     5881 gttcctggcg atagacctgc gtttccacca atatgtccca agtgaaaaat aatatggtta
     5941 caaagcaaca caagaagaaa aagtagagga agtaaattcc tagactggca aataaaaact
     6001 gaaagagtaa gacaagaaat gccaaagaaa gcagatagat aaaaagacga ctacgggagc
     6061 gcagataggc tagaaaaaat tgtttccaat caagcatgct tcaatccgta ccctattcct
     6121 ttcttggtct cgataaatcc taccaatccc tgctcctcca actttttacg caaacgagcc
     6181 acattgacag agagggtatt atcatcaatg aaaaagtcac tgttccaaag ttcccgcatc
     6241 aggtcgtcac gtgctacgat gttgcctgca tgctcaaata acacgcgtaa aatctggaat
     6301 tcattcttgg tcaaattcaa gacttgccct tgataatgta aatccatgga tttggtattg
     6361 aggataacac cagcatattc cagcaaactc tcatcacgcc caaactcata ggaacgacgc
     6421 aacaagccct gaaccttagc taaaagaacc tgctggtcaa aaggcttggt cacaaagtca
     6481 tccgccccca tattgattgc catgacaata tccatagcct ggtctctcga agaaagaaac
     6541 atgataggta ccttggaaat cttgcggatt tcctgacacc agtgataacc attaaacaag
     6601 ggcaaaccaa tatccatgag gaccagatga ggttccgact gaacaaatag actcaaaact
     6661 tccataaagt cttctaccag gaccacttca aatccccatt cagagagcat tttcccaatc
     6721 tgttgacgaa tgacctgatc atcttctatt aataaaatct tgtgcatgcg cttctccttt
     6781 tccattatta taacagattt ttccatgcta gatggtctga aactgaattt gaaatagcct
     6841 gtttttagcc agtacaaaca ggctatgcta ctagctaatt tgagggaaat ttgctaagat
     6901 aaataaaaag aaaggagctc ttatggccaa tatttttgac tatctgaaag atgtcgcata
     6961 tgattcttat tacgaccttc ccttgaatga gttagacatt ctaaccttaa tagaaatcac
     7021 ctacctctcc tttgataatc tggtctccac acttcctcaa cgtcttttag atctagcacc
     7081 tcaggttcca agagagccca ccatgcttac tagcaaaaat cgccttcaat tattagatga
     7141 attggctcaa cacaagcgct tcaaaaattg caaactctcc cattttatca acgacatcga
     7201 ccctgaactg caaaagcaat ttgcggctat gacttatcgt gtcagcctcg atacctatct
     7261 gattgtcttt cgtgggacag atgacagtat cattggctgg aaggaagatt tccacctgac
     7321 ctatatgaag gaaattcctg ctcaaaagca cgcccttcgc tatttaaaga acttttttgc
     7381 ccatcatcct aagcaaaagg ttattctggc tgggcattcc aagggaggaa atctcgctat
     7441 ctatgctgct agccaaattg agcaaagttt gcaaaatcag atcacagcag tttatacatt
     7501 tgatgcacct ggtctccatc aagaattgac acagactgcg ggttatcaaa ggataatgga
     7561 tagaagcaag atattcattc cacaaggttc cattatcggt atgatgctgg aaattcctgc
     7621 tcaccaaatc atcgttcaga gtactgccct gggtggcatc gcccagcacg atacctttag
     7681 ttggcagatt gaggataagc acttcgttca actggataag accaacagtg atagccggca
     7741 agtagacaca acctttaaag aatgggtgac cacagtccct gacgaagaac ttcagctcta
     7801 cttcgacctc ttctttggca ctattcttga tgctggtatt agctctatca atgacttggc
     7861 ttccttaaag gcgcttgaat acattcatca tctctttgtc caagctcaat ccctcactcc
     7921 agaagaaaaa gaaaccttgg gtcgccttac ccagttattg attgatactc gttaccaggt
     7981 atggaaaaat agataatact cttgaaaatt aaatgtatac aaaacaaaag acctagaata
     8041 catactttca tgtgcattct aagtcttttt aaatagaatc taatagtcaa taaaaatcaa
     8101 agagcattga gagataatgg ggcttggaac gtccctctcg cttcaacaaa atgaccccat
     8161 tatagattaa aaagatgcca cttagaaaaa gcaaaaaaag gaagtaatac aaaggcaaat
     8221 acataaaaag ctaactgaac attctcgtat ccatttttat aaaaaaggta ggatag
//
LOCUS       DAVMEF010000083         8240 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMEF010000083 DAVMEF010000000
VERSION     DAVMEF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.083"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..198
                     /locus_tag="VYQ30_001336"
     CDS             <1..198
                     /locus_tag="VYQ30_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEV1966441.1"
                     /translation="EGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKP
                     DGTLADKPEFTVEPDGLITVK"
     gene            complement(468..647)
                     /locus_tag="VYQ30_001337"
     CDS             complement(468..647)
                     /locus_tag="VYQ30_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966442.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(789..938)
                     /locus_tag="VYQ30_001338"
     CDS             complement(789..938)
                     /locus_tag="VYQ30_001338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966443.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            1220..1687
                     /locus_tag="VYQ30_001339"
     CDS             1220..1687
                     /locus_tag="VYQ30_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1966444.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            1720..1817
                     /gene="ffs"
                     /locus_tag="VYQ30_001340"
     ncRNA           1720..1817
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYQ30_001340"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1874..2317
                     /locus_tag="VYQ30_001341"
     CDS             1874..2317
                     /locus_tag="VYQ30_001341"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1966445.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            2319..2834
                     /locus_tag="VYQ30_001342"
     CDS             2319..2834
                     /locus_tag="VYQ30_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1966446.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            2848..4209
                     /gene="radA"
                     /locus_tag="VYQ30_001343"
     CDS             2848..4209
                     /gene="radA"
                     /locus_tag="VYQ30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1966447.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQLGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            4282..4779
                     /locus_tag="VYQ30_001344"
     CDS             4282..4779
                     /locus_tag="VYQ30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1966448.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            4804..5013
                     /locus_tag="VYQ30_001345"
     CDS             4804..5013
                     /locus_tag="VYQ30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966449.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            4995..5618
                     /locus_tag="VYQ30_001346"
     CDS             4995..5618
                     /locus_tag="VYQ30_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1966450.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFPGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            5763..6731
                     /locus_tag="VYQ30_001347"
     CDS             5763..6731
                     /locus_tag="VYQ30_001347"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1966451.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6865..7146)
                     /locus_tag="VYQ30_001348"
                     /pseudo
     CDS             complement(6865..7146)
                     /locus_tag="VYQ30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7273..7506)
                     /locus_tag="VYQ30_001349"
     CDS             complement(7273..7506)
                     /locus_tag="VYQ30_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966452.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(7499..7615)
                     /locus_tag="VYQ30_001350"
     CDS             complement(7499..7615)
                     /locus_tag="VYQ30_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1966453.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(7830..8180)
                     /locus_tag="VYQ30_001351"
     CDS             complement(7830..8180)
                     /locus_tag="VYQ30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1966454.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
BASE COUNT         2271 a         1465 c         1929 g         2575 t
ORIGIN      
        1 gaaggtgcca tgaagacagg ctgggtcaag tacaaggaca cttggtacta cttagacgct
       61 aaagaaggcg ccatggtatc aaatgccttt atccagtcag cggacggaac aggctggtac
      121 tacctcaaac cagacggaac actggcagac aagccagaat tcacagtaga gccagatggc
      181 ttgattacag ttaaataaat agaaaggaaa ctttctaaat tgttctttca ccgcaggctc
      241 aggcttgcgg tttttttgtt tgtctgaatc aagaaaacgt ctaaccaacc gacaccaatg
      301 tcggtagcaa aataaatggt ttgcctaaaa aattagagtt aagttctatt tttcaaaaac
      361 acgcattttg aacgattaga aacagaaatc taaatcctat tgttcaaaaa aacgtttaca
      421 tgaaaaatag ggaggtgttt tgttaaaaat aaaaacagtg atcgaaatca ctgcttatca
      481 gttgtagcaa attcatagag tttttctgct gtgagaagtg ccattttgtc catgcttgtt
      541 tttccttttc tgaggtcaga aacagtagtc catggaactc cagcaccttg ggaaatagca
      601 gatgtagaca tcgaactgtc taacaattct tgaataactt ctctcatctt atttgtcctt
      661 tttatttttt agataaatat atacattgat tacaattata aaaatagcta ttgcactaac
      721 cattgcattt cctctttcca tttgataaaa tagaggtgta aggggctttc gccccaacct
      781 cttagcgttt acctttttct ttgacgggat ttcggtttac gctttttgtt ttgccttgcg
      841 accgttattg cggtcactag acttgcgata gcagttaccg tttcaggaat attgtctatt
      901 gccttttcaa gtaacctgag ccaatcttct ttgttcaact tcatcacccc ctttccttat
      961 cttgattata ttatatcacg gtacgccgag aaagtcgagc gttttgatga agttttttta
     1021 attttttcaa aaaaaaaata gaccttgtcc agaggtcggg gagttggagg gggcaccctc
     1081 caaaagcatt gatttaataa ggttttattt tacctttttc ataataatct ccctatagag
     1141 tcaccgcatt cggtggcttt ttttgtgttg ggattcatga tataataata aaatcgataa
     1201 gtaggaaaag agaaaagaga tgtattatac gcttgaagaa aaagaagtct ttatgaggga
     1261 ggctttgaga gaggctgaga ttgctcttga acacgatgaa attccaattg gttgtgtgat
     1321 tgtcaaagat ggggaaatca ttggtcgtgg gcataatgcg cgtgaggaat tacagcgagc
     1381 ggttatgcat gcggaaatta tggctataga ggatgcgaac ttgagtgagg agagctggcg
     1441 cttgctggat tgcacacttt ttgtgaccat tgaaccttgt gtcatgtgta gtggggcgat
     1501 tgggcttgcc cgtattccaa atgtggtcta tggggctaaa aaccagaaat ttggcgctgc
     1561 tggaagtttg tacgatatct tgacagatga gcgtcttaac catcgtgtag aggttgaaac
     1621 gggaattttg gaagatgaat gtgcagctat catgcaggac ttttttagaa atagacggaa
     1681 aaaataattt tgcttttaaa atgaatagga atgtgatata ataaatagtg gagcaacagt
     1741 tctgcgtgaa gcgggtcagg ggaggaatcc agcagcccta agcgatttga attgtgtgct
     1801 ctttttttcg tgctttttcc gaataaataa gatagaataa tctagaataa atgataatag
     1861 aaaagagaaa attatgaaaa ttcgtggttt tgaattggtt tcgagtttta cagatgaaaa
     1921 tttattgccc aagcgtgaga cagcgcatgc ggctggttac gacttaaagg ttgctgtgcg
     1981 tacagttgtt gcgccaggag agattgtctt ggttccgaca ggggttaagg cttatatgca
     2041 gccgactgag gttctctacc tctatgatcg ttcttcaaat cctcgtaaga agggcttggt
     2101 tttaattaac tcagttgggg tcattgatgg ggattattat ggaaatcctg gaaatgaagg
     2161 gcatattttt gcgcagatga agaatatcac agatcaagag gttgttcttg aagttgggga
     2221 acgtattgtc caggctgttt ttgctacttt cttaattgca gatggagatg cagctgatgg
     2281 cgttcgaact ggtggatttg gatcgacagg gcactagaat gaagattatc tttgtacgtc
     2341 atggggagcc agattaccgt gagttagagg agcgttctta tataggattt gggatagatt
     2401 tggcaccctt gtctgagatg ggacggcagc aagtccagaa attgagcaaa aatcctttac
     2461 tctcgtcagc tgaaataatc gtatcttctg cagtcacaag agctttagaa acggcttcgt
     2521 atgtggtctg tgctacgggt cttcctttaa gagtagagcc tttattacat gaatggcagg
     2581 tctataaaac aggaatagaa aactttgaaa cagctagaag actgttttta gaaaacaagg
     2641 gggagttgct tcctaatagt cctattcaat atgagacagc tacggaaatg aagtctcggt
     2701 ttctagaatg tatgtctaag tatcgagaac atcagactgt ggtagttgtt gctcatcgaa
     2761 tgctcatgcg ccagtttgtg ccaaatgaga agattgattt ttgccaagtg attgagtgtg
     2821 agttagagat atagaaagag gtttgtcatc gcaaagaaaa aagcgacatt tgtatgtcaa
     2881 aattgtgggt ataattcccc taaatatctg ggacgttgcc ccaactgtgg gtcttggtct
     2941 tcttttgtgg aagaggttga ggttgccgag gtcaagaatg cgcgtgtgtc cttgacaggt
     3001 gagaaaacca agcccatgaa actagctgag gtgacttcca tcaatgtcaa tcgaaccaag
     3061 acggagatgg aggaattcaa ccgtgtgctt ggaggcggag tggtaccagg aagtctcgtc
     3121 ctcatcggtg gggatcctgg gattgggaaa tcaactcttc tcctacaagt ctcaacccag
     3181 ttgtcccaag tggggacagt tctctatgtc agtggggagg agtctgccca gcagattaaa
     3241 ctacgtgcag agcgcttagg tgatattgat agtgagtttt atctctatgc agagaccaat
     3301 atgcagagtg ttcgtgcaga agtggagcgt atccagccag actttctcat tattgattcc
     3361 atccagacca tcatgtctcc tgagatttca ggggtgcagg ggtctgtttc tcaggtacgt
     3421 gaagtgaccg ctgaactcat gcagttggcc aagaccaata acattgccat ctttatcgta
     3481 ggtcatgtga ccaaagaagg aaccttggct gggcctcgta tgttggagca tatggtggat
     3541 acggtgcttt actttgaagg ggagcgtcac cacacctttc gtattttgag agcggtcaaa
     3601 aatcgttttg gttccactaa tgagattggg atttttgaga tgcagttggg cggcttggtt
     3661 gaggtactca atccgagtca agttttccta gaagagcgtt tggatggggc gactggttcc
     3721 tccatcgttg taaccatgga agggacgcgt ccgattttgg cggaggttca ggctttggta
     3781 acaccgacca tgtttggaaa tgccaagcgt acgacgacag gacttgattt taaccgtgct
     3841 agcttgatta tggctgtttt ggaaaaacgg gcagggcttc tcttgcaaaa tcaggatgcc
     3901 tatctcaaat ctgctggtgg tgttaaattg gatgaacctg cgattgactt ggctgttgca
     3961 gttgctattg cttcgagcta caaagacaag ccaactaatc ctcaggaatg ttttgtcgga
     4021 gaactgggct tgacaggaga gattcggcgc gtgaatcgta ttgagcaacg catcaacgaa
     4081 gctgctaaac tgggctttac taagatttat gtacctaaga attccttgac aggaatcact
     4141 ctgcctaagg aaattcaggt cattggcgtg acaacgattc aggaagtttt gaaaaaggtc
     4201 tttgcataat ccgtgacaaa ttctcttaaa aatgataaga taggagaaat atttgactat
     4261 caaattttca aggagggaat cgtgtcgtat tttgaacagt ttatgcaagc caatcaggct
     4321 tatgttgccc tacatgggca gttaaatctg ccacttaaac ctaaaaccag agtagccatt
     4381 gtgacctgta tggactcacg tttgcacgtt gcgcaagctc taggtttggc acttggggat
     4441 gctcatatct tgcggaatgc aggtggtcga gtgactgaag acatgattcg ttcgctagtt
     4501 atttcccagc aacaaatggg gacaagagag attgtggtat tgcaccatac agactgtggt
     4561 gctcagacct ttgaaaatga accttttcag gagtatttaa aagaggaatt aggtgtagat
     4621 gtgtcagacc aggacttctt gcccttccaa gatatagaag agagtgtacg cgaggatatg
     4681 caactgctta tcgagtctcc cctaatacca gacgatgtca ttatctctgg tgccatttac
     4741 aatgttgata caggaagtat gacagtcgta gaattataaa tacttcattt agaaagaaag
     4801 tgtatgaaga aaagcagtat tttatttatt tttattttat tgctatgtat tggtttacag
     4861 tatgaaacca tctactatac ggacggttcg aggtcaggtg cggaatatgg actaatggga
     4921 gtttctatct ttctagctct cttttacatg attccggctc tttattttct cttccgtatt
     4981 gggaaaaatg ggaattgcca aagaaggttt tgattctgtc tttattggga gggatgttcc
     5041 tttcaggctg gttgtctagt tttgctaata cttatatcca tgatttactg ggggttcttt
     5101 tcccagatag tacattttta aatgcctttg aaagtgctat tgtggctcct ttggtagaag
     5161 aacccttgaa attattgcca cttgtttttg ttttggcttt gattcctgtg cgaaaattaa
     5221 aatctttgtt tttacttgga attgcttccg gtttgggatt ccaaatgatt aaggatattg
     5281 gttatattcg tacggatttg ccagagggct ttgactttac tatttcgcga attttagagc
     5341 gtatcatctc aggaattgcc tctcactgga ctttttcagg tctagctgta gtaggtgttt
     5401 acttgcttta cagagcctat aaaggacaga aggttggcaa gaaacagggc cttatttttc
     5461 caggtttagc cttgggaact cacttcttgt ttaactctcc ttttgtggag ttggaaacag
     5521 agttgccttt agcgattcca gtggttacgg ctattgctct ctatggtttt tatcatgctt
     5581 attgctttgt tgagaaacac aatgagttga tgacctagaa tatttttcaa aagaatgatg
     5641 caagggtaca aatatggtgc ccttctttta tttttgattg aaaaatagtg caaaaagcgc
     5701 tacaatggta gatggaaaat cttgtgaaaa gcacaagtta tacatatata ccggaggaaa
     5761 tcatgtcttt ttctgattta aagctgtttg ccctttcttc taataaagaa ttggcagaac
     5821 gtgtggcgca ggagattggg atagagttgg ggaaatcaag tgttcgccaa ttttcagatg
     5881 gagagattca ggtcaacatt gaagaatcaa tccgtgggaa acacgtcttt atcctacaat
     5941 caactagttc gcctgtaaat gacaatctgc ttgaaatttt gattatggta gatgctttga
     6001 agcgtgcgag tgcagaatct gtcaatgttg tcatgcctta ctatgggtat gcacgtcagg
     6061 atagaaaggc gagagcgcgt gagccaatca cttcaaaact tgtcgcaaat atgcttgaag
     6121 tagctggagt ggatcgttta ttgaccatcg acttgcatgc tgcgcaaatt caaggattct
     6181 tcgatattcc tgtggatcat ttgatgggtg ctcctctaat tgcagattat tttgagcgtc
     6241 gtggtatggt tggttctgac tatgtggttg tcagcccaga ccatggaggg gtgactcgtg
     6301 ctcgtaagtt ggcagaattt ttgaaaacat ctatcgctat tattgataaa cgtcgtagcg
     6361 ttgataagat gaatactagt gaagttatga acatcatcgg taaggttgaa ggcaagactt
     6421 gtatcttgat tgatgatatg attgataccg ctggaacgat ttgtcatgcg gcagatgctc
     6481 ttgcggaagc tggtgctgtt gaagtctatg caagctgtac gcacccagtt ctttctggtc
     6541 ctgctacgga caatatccaa aaatcagcta ttaagaaatt ggttgttttg gataccatct
     6601 atctgccaga agagcgtttg attgataaga ttgagcagat ttcaatcgct catctcctag
     6661 gggatgctat cgtacgtatt catgaaaaac gaccactttc tccacttttc gatattgaga
     6721 aaaaaattta atgaccaagc ctgagatgat tctcaggttt tttcgtcttt tttccgaata
     6781 aatagatagt agcggtgaat ctagtaaagc tagatttaaa actgtgatat aataaaaaga
     6841 gaagaaatat gactgtacgt catcagaagt ttcagcgacc atcatttttg aacagtgata
     6901 gcacttgaat cgacgctttc taaggagaat tctagtaggt ataccagtcg tttcaagata
     6961 aggaatttta gaaggttttt gaaagtcata tttcttcaat tggtttccgc actcagggca
     7021 agatggggcg tcgtagtcca gtttggcgat gatttccttg tgtgtatcct tattgatgat
     7081 gtttaaaatc tggacattag ggtctttaat gtctagtaat tttgtgataa aatgtaattg
     7141 ttccatatga ttctttctaa tgagttgttt tgtcgctttt cattataggt catatgggac
     7201 ctttttttta cacaaaatag gctccataat atccatagtg ggtaaatccc ctatggatat
     7261 tatggagcca ttttacaaca gtgcctcaaa ttcagatact gacataccta attgctggct
     7321 ggcaacctca gaagtcagaa gaccttggcg tactatgtct aggaaggcaa aaagtttcct
     7381 ttctgccctt ccttcaactt tcccacgctc tagtccttgt tcaaggcctt cagccctagc
     7441 agtttccaag gcatagtcct gtgctaacaa gacttgttct tctcgcatat gtagttgact
     7501 aaacattttc ctgtcctcct cggaccagct cttgtagtcc agcagttgat ttgcttgggt
     7561 aatggctcgt tgcggttgct gggtaaaggg cttgttgccg aaaaactcca accatggctt
     7621 gcgaaccttg tctttgctgg tttctctgta ttttttaatt ccaagaatgc catcttaacc
     7681 agatggtttt cctgtccatt gttggtaatc gccaatacct cacctgttgt gtcttcgcgc
     7741 atactaaagc tatgaaaagc caggtcatct gagaaataat tactatccac gatagcaatg
     7801 acgtaaacag gagcgatgtg tttgtagatc tagtgagtat caccttctcg ctgacgaatt
     7861 ttttcaagat tttgattaac ctgactgcac aggtaagccc acaagtgatt gatgaaaaaa
     7921 ttctgatgat ggacttgaat ctcaataatt acttgagtac cgttatccaa ctccgccaag
     7981 acgtctatac tggtataaaa atcctgcacc gagtaaggca tggagagtaa tacgtgaata
     8041 tcgcttccct ccaaaatggt cacatttttt gctggcaagt ccagcatatc gcgaataaat
     8101 tgacaagtga tttctggatt gctgaatatt ttcttagcaa tcaaatcatt agttgggctg
     8161 atgcccggat gtctgagaat cctcctattc ctccttctat agtgagttga aataagatgt
     8221 gaataactct atcaggaaag
//
LOCUS       DAVMEF010000084         8204 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMEF010000084 DAVMEF010000000
VERSION     DAVMEF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.084"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(101..2098)
                     /gene="metG"
                     /locus_tag="VYQ30_001352"
     CDS             complement(101..2098)
                     /gene="metG"
                     /locus_tag="VYQ30_001352"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1966455.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2222..3481)
                     /locus_tag="VYQ30_001353"
     CDS             complement(2222..3481)
                     /locus_tag="VYQ30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966456.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSISVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3483..4184)
                     /locus_tag="VYQ30_001354"
     CDS             complement(3483..4184)
                     /locus_tag="VYQ30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966457.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4168..5367)
                     /locus_tag="VYQ30_001355"
     CDS             complement(4168..5367)
                     /locus_tag="VYQ30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1966458.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PIHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5632..6978
                     /gene="gor"
                     /locus_tag="VYQ30_001356"
     CDS             5632..6978
                     /gene="gor"
                     /locus_tag="VYQ30_001356"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1966459.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAASEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            7175..7711
                     /locus_tag="VYQ30_001357"
     CDS             7175..7711
                     /locus_tag="VYQ30_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1966460.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7762..7848
                     /locus_tag="VYQ30_001358"
                     /pseudo
     CDS             7762..7848
                     /locus_tag="VYQ30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7962..8177)
                     /locus_tag="VYQ30_001359"
     CDS             complement(7962..8177)
                     /locus_tag="VYQ30_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1966461.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
BASE COUNT         2334 a         1812 c         1558 g         2500 t
ORIGIN      
        1 gtgttttgag caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
       61 ataaaaaaac agaactagcc tgaactagtc ctgtctactt ttacccaatc acacttccat
      121 ttggtacagc tggatcaact gtgagaaggg ttaatttgcc atcatgttca gctgagagaa
      181 tcataccctg gctgacatat tttttcatca ttttacgtgg tttgaggtta gcaacgattt
      241 gaactttctt gccgaccaat tcttgttcat ttggatagta ttttgcaatt cctgaaagaa
      301 tctgacgatc ttctccatca ccagcatcca agcggaattg aagcaactta tctgaacctt
      361 ctactttaga cacttctttg acttctgcga cacggatttc aaccttgtca aagtcttcaa
      421 acttgatttc atccttgttt agtttgagct caacttcgtc cggattccat tctttttcga
      481 ctgctggttt attgccttcc atttgttcct tgatataggc gatttcttct tccatattta
      541 gacgtggaaa gataggtgtt cctttggcaa ctacagtcac atctgctggg aagtcagcca
      601 aactcaagtt ttcaagacta gaaacttctt ccaaaccaag ttgagtcaaa actgcacgac
      661 tagtttccat cataaatggt tcaatcaagt gagcaactac acgaatgctg gctgccaagt
      721 ggctcatgac acttgccaat tggtcacgaa gagcttcatc cttgtccaag acccatggtg
      781 cagtctcatc gatgtattta ttggtacgag agatcagagt ccagactgct tcaagcgcac
      841 gtggatagtc aactgcttcc atgtgtgtat ggaagtctgc gattgattgt tctgcaacct
      901 cagcaagaac atgatcaaat tcagtcacac cttctacata ggcagggatt tgtccatcaa
      961 agtacttatt aatcatggaa accgtacggt taaggaggtt cccaaggtca ttagccaatt
     1021 catagttgat acggccgaca tagtcttcag gagtaaaggt tccgtctgaa ccaactggaa
     1081 ggttacgcat gaggtagtaa cgaagtggat ctagtccata acgctctacc aacatttcag
     1141 ggtaaacgac attccctttt gacttagaca tttttccgtc tttcatgaca aaccaaccat
     1201 gggcaatcaa acgatcaggt aatttaacat ccaacatcat aagaaggatt ggccagtaga
     1261 tagagtggaa gcgaaggata tcttttccta ccatatggaa gactgttcca ttccagaact
     1321 tgtcaaagtt accatgttcg tcttgagcgt agccaagagc tgtcgcatag ttaagaaggg
     1381 catcaatcca aacgtagaca acgtgttttg gatttgatgg gacaggcact ccccatgtaa
     1441 aggttgtacg agataccgcc aaatcttcca aacctggctc gatgaagttg cgtagcattt
     1501 cattaaggcg accatctggc gtgataaatt caggatgagc tttgaaaaat tcgaccaaac
     1561 ggtcttggta tttgctaagg cgaaggaagt atgattcttc agaaacccat tcaacctcat
     1621 gacctgatgg agcaatacca ccagtcacat ttccagcttc atcacggaaa acttctgcca
     1681 gctggctttc tgtaaagaat tcttcgtctg atactgaata ccaaccagag tattcaccca
     1741 agtagatatc atcttgagca agtaagcgtt caaagacctg tgcgacaact ttttcatggt
     1801 agtcatcagt tgtacggata aatttatcgt atgagatatc tagtaattgc cagagttctt
     1861 taactccaac cgccattcca tcaacatagg cttgaggtgt aataccagct tcttccgctt
     1921 tctgctggat tttctgacca tgttcatcaa gacctgtcag ataaaataca tcgtagccca
     1981 tcaggcgttt gtaacgtgct aggacatcac atgcgatagt tgtgtaggca gaaccgatat
     2041 gaagtttccc agatggatag taaatcggcg ttgtaatata aaaatttttt tcagacataa
     2101 tttttccttt ccaggcaaat gaaacctgtt tttctaacac ttcattatat cacattttta
     2161 atgaatttcg atagggaaat ccatacaaaa acaagataga cgagtgtcca tcttgttgat
     2221 ctcattcata acgaagggct tcaattggat caagtttcga tgccttgttg gctggcaaga
     2281 ctccaaaaat cataccaaca ctagccgaaa ctgcaagact aaatagggca actgagattg
     2341 atactccaac ttctatacct tctattaaac cttgcagtaa caaacctgct aaggcagtta
     2401 aaccacttgc aattgtcaag ccaattaagc cacctaacaa ggtcaaaatc atggattcaa
     2461 tcaaaaactg aattaaaata ttggcacgtg ttgcacccaa agccttacga agaccaatct
     2521 cacgagtgcg ctctgtcacc gaaaccagca tgatgttcat gacaccagtt cctccaacaa
     2581 agagagaaat ccctgcgatg gaactaataa tcgtcgtcat aaaactaaac gattgttgaa
     2641 tttctgcaaa tacaagggac tcatctgcca cctggtattc tccctgttgt aagcctgcaa
     2701 gctctgtcat ttttcgtgcc agttctggac ccagagttgg ggttaaactg gtatcattca
     2761 ctcgaaagac aatattagct atttcatcta cattaaaatt cgcagcaagg gagatattgg
     2821 tagtaatagg caagccacca aacccatata tttttgatct tttagcctcc ggactagtat
     2881 aaaccccaat gacccggtaa ctaaatccat tgacttctac aaccttgtta atagcctctt
     2941 gaggagattc aaataaacta atggacaatt cctcatctag caaaatgaca cttgcaaact
     3001 ctttgaaatc ttgctctctc agactacgac ctgcaataat ttcattctta acagcgtcca
     3061 tgtaagttct gtttccacct gtcaaattag cattctcaac ctttttatct tgataggtca
     3121 agatggcatt cgttgaattg gttacatagt aactatccac tcccttcagt ttagctgcct
     3181 cttggaccca ggattcttgc ggttttggcg gttcaacagg aacttcctct tcctttccag
     3241 aaaccgtaaa agctgattgt ttctgagtaa aagacccgtc tttacttttt ttaggagaga
     3301 aaaagacgct aatatttttc tgagatttag tcatatcttt attgacttga cgagataggg
     3361 aatcacccaa agccataatc acaacaactg atgaaacacc gataataatc ccaatcatag
     3421 taagcaaaga acgcatcttg tgagccatga tagatgaaaa ggcaaatttc agattctgca
     3481 tcttagtttt cctcctttcc taactgagca ctgtcagacg aaatgacccc atcccgaatg
     3541 acaatctgac gtttggcata ggcagcaatc tcaggctcat gcgttaccat gataatggtt
     3601 tttccttctt tattcaaatc aaccaataat tgcataattt ggttacctgt tttggtatcc
     3661 aaggctcctg tcggttcatc cgctaggata atagaaggat tgtttaccaa ggcacgcgca
     3721 atggctacac gttgcttttg accaccagat aattctgaag gtaaatggtg actacgttct
     3781 gtcaattcaa ccttgtctaa atattcctca gccaacttgc gacgttttga agacgaaact
     3841 cctgcgtaaa tcaagggcaa ttctacattt tgcagagcat tgagcttcga tagaagaaag
     3901 aactgctgaa agacaaaacc gatttgttgg ttacggacct tagctagttg tttttcacca
     3961 agcccagcca cttcttgacc ttcaagataa tattctccac tggttggtgt atccaacatg
     4021 ccaatcgtat tcatcagagt ggacttacca gacccagatg gtcccatgat ggctacaaat
     4081 tcaccctcat tcacttctag attgatattt ttgagaacct gcagttcttg gtcaccatta
     4141 cggtaacttc tgaagatatt ttttagacta attagttgct tcatcagcct tcacctcttt
     4201 tccttcttcc aaggaagatg ttggattact gatgacctta gcaccgttcg ttaaaccaga
     4261 agtgatttct tgattttctg cgtcagcatt tcccaatgaa acctcaactt ttttagcctt
     4321 ttgttgttca tccacaatcc agacataatt tttactatca tccattacta gactgctaac
     4381 aggaacaaga atagccttag ttttgctttt aacctcaatg ttgacagaaa aaccttgttt
     4441 caaatcacca acctcgcctg tcacatcaat agtataaggg tatttagaac ctgtattatt
     4501 cccggctgct ggactagctg cttcaccatt gtttttagga tagtcagaaa tatagcttaa
     4561 tttcccagtc cattttttat caggatacac tttagaagta aagcttactt cttgacctac
     4621 agaaaggttg gctagattgt actcagacaa ttctcccttg acttgtaaat tttcattgct
     4681 gacaatatga accataactt ggctcgcccc tgttggagat ttagaaacat tgctattgac
     4741 ttcgactaca gttccctcta gggtactgag aacagttgtt gcatccaatt gactttgagc
     4801 cttgcttaat tgcgccgcag catctgcacg cgcatcacgg gcatcaccca attgagcgtc
     4861 aatagaagca acagaatttc cagccactgg agttgggctt tgcaccgttg catcttctcc
     4921 tcctactggc gctggtaact gtggagccgg agctgaagcg gcttcatttc gtgcttgatt
     4981 gagttcattg atatgacgat ctgccctagc tactgctcga ctagctgaat cataggccgc
     5041 ctgcgcttct gaactactgt acttgactaa agcctgccct tcgctgacct tatcgcccac
     5101 agaaacaagg atttcatcta aatcaccctt actagcatca aaataaacat attgttcatt
     5161 ttttgctgtt actgtccctg acaataaaac agaggaggcc acgcttcctt ccttggcaac
     5221 aacaagatga ataggctcat cttttagagc agtctgagaa ggttgtctaa agagtaaaat
     5281 ccccccagca cccaatacaa ctacactcgc agcaccgatt gctgcataca gttgccactt
     5341 tttagcttta ccattctttt tcttcataat gaaactcctt ttctttttta caatactttg
     5401 ctattatacc aaatttccct ccagcaaaca atacagttca ggattaaaca atcgttcgga
     5461 attttgcttt tctattgtac tagatatata atacactatc ttgacttatt tggcaagctt
     5521 ttttgcaatt ttttgtgcgt agcagatttt tgcaatcaaa tcaaaaaaag aataaaatat
     5581 aactatcaaa aaagacgctc tactatttaa aagattacaa aggagttttc aatgagagaa
     5641 tatgatatca ttgctatcgg tggaggtagc ggaggaatcg ctaccatgaa ccgtgctggt
     5701 gaacatggag ccaaagcagc cgttattgag gaaaagaaat taggtggaac ctgtgtcaac
     5761 gtcggttgtg ttcctaaaaa aatcatgtgg tacggggcac aaatcgctga gactttccat
     5821 caatttggag aagactacgg ctttaagact actgatctta actttgactt tgcaacccta
     5881 cgtcgcaatc gtgaatccta cattgatcgc gctcgttctt cttatgatgg cagttttaaa
     5941 cgcaacggtg tagacttgat tgaaggtcat gctgaatttg tagattctca tactgtaagc
     6001 gtaaatggtg aactgattcg tgctaaacat atcgtgattg ctacaggtgc ccatccaagt
     6061 attcctaata ttcctggtgc tgagctaggt ggctcttctg atgatgtatt tgcctgggaa
     6121 gaacttccag agtcagttgc cattctaggc gctggttata tcgccgttga attagctggc
     6181 gtactccaca cttttggtgt caagacagat ctctttgttc gccgcgatcg tcctttacgt
     6241 ggttttgatt cctacatcgt tgaaggtttg gtcaaggaaa tggaaagaac aaacttacca
     6301 cttcacactc acaaagtccc tgtcaagtta gaaaaaacta ctgacggcat taccattcat
     6361 ttcgaagatg gtactagtca cacagctagc caagttatct gggctacagg ccgccgtcca
     6421 aacgttaagg gcttgcaact tgaaaaagct ggagtgactc tgaatgaacg tggctttatc
     6481 caagtagatg aataccaaaa taccgtcgtt gagggaatct atgctctagg tgatgtaacg
     6541 ggcgagaaag aactgactcc agttgcaatc aaggccggac gtaccctatc tgaacgtctc
     6601 tttaacggaa aaactactgc aaaaatggat tactcaacta ttccaactgt tgtcttttca
     6661 caccctgcta tcggaactgt tggtttgaca gaagagcaag ctattaaaga atacggtcaa
     6721 gaccaaatca aggtttataa atcaagcttt gcatctatgt actctgcttg cactcgcaac
     6781 cgtcaagaat cccgtttcaa actcataaca gctgcttcag aagaaaaagt tgtcggactt
     6841 cacggaattg gctacggcgt tgatgaaatg attcaaggat ttgctgttgc tatcaaaatg
     6901 ggagcaacca aggctgactt tgacgcaact gtagcgattc acccaacttc atctgaagaa
     6961 tttgtaacca tgcgttaatc gaaacaaaag aagctgatat aaatatgtct gcttcttttt
     7021 taatgatttt cgaatcaaca aaaaaattat ccaacttgac tagtcacgaa aatcggctgt
     7081 tacagttttt gttaccttgt ttttaaaaaa tggttgacat cgactcctat atgagtataa
     7141 tagttactat acattagaaa agaggttaac tattttgaaa aaagctcacg tttatgctat
     7201 ccctgctatt ggggctgctc tcattgctgc tttggcacaa atcagtcttc caattggacc
     7261 tgttcccttc actctgcaaa actttgcaat cggcttgatt gctactgtct ttagaccgag
     7321 agaggctgta ctttctgctg gactctatct tcttctaggt gctatcggtc ttcctgtctt
     7381 tgcaggaggt ggagctggtt ttcaggcttt agttggccct actgcaggct atctttggtt
     7441 ttatctcgtt tactctggac ttacttcctc tctaaccaac agcaagagtg gtgttgttaa
     7501 gatttttctt gcaaacctct tgggtgatgc ccttgtcttt gtcggcggga ttctcagctt
     7561 gcatttccta gctggaatgg catttgaaaa agctcttgtt gtgggggttc ttccctttat
     7621 cattccagac cttggcaaac ttctggctat tagttttatt agccgtcccc tacttcaacg
     7681 ccttaaaaat caggcttact ttactaacta aaaaaggata ccgagttatc atgactcaat
     7741 atccttttct tttattttga aaacttatac tcaatgaaaa tcaaagagca aactaggaag
     7801 ctagccgcag gctgctcaaa acactgtttt gaggttgtgg atgaaactga cgaagtcagc
     7861 tcaaaacatt gttttgaggt tgtggatgaa actgacgaag tcagtaacca tacatacggc
     7921 aaggcaaagc tgacgcggtt tgaagagatt ttcgaagagt attagttgcc tttaaaggca
     7981 tccaccatcg tttgaaattc ttcatttgag agagtaatcc ctttgcccat tttagtatgg
     8041 tctggactcc aagcacgaat atcaaacttt gcaggggcac cattaaagct cacacggtta
     8101 atttccttgg tccaaccttt ttcgttttca gaaagagtca acaagtgctc ttcgatttca
     8161 aatgtaaatt ctgccatttt cttctccttt tttagtttca ttag
//
LOCUS       DAVMEF010000085         8138 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMEF010000085 DAVMEF010000000
VERSION     DAVMEF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.085"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            49..696
                     /locus_tag="VYQ30_001360"
     CDS             49..696
                     /locus_tag="VYQ30_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1966462.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            827..1240
                     /locus_tag="VYQ30_001361"
     CDS             827..1240
                     /locus_tag="VYQ30_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966463.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1259..1444
                     /locus_tag="VYQ30_001362"
     CDS             1259..1444
                     /locus_tag="VYQ30_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966464.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1711..2154)
                     /locus_tag="VYQ30_001363"
     CDS             complement(1711..2154)
                     /locus_tag="VYQ30_001363"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1966465.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2184..4406)
                     /locus_tag="VYQ30_001364"
     CDS             complement(2184..4406)
                     /locus_tag="VYQ30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1966466.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5365..5994
                     /locus_tag="VYQ30_001365"
     CDS             5365..5994
                     /locus_tag="VYQ30_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1966467.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6117..8009)
                     /gene="pepO"
                     /locus_tag="VYQ30_001366"
     CDS             complement(6117..8009)
                     /gene="pepO"
                     /locus_tag="VYQ30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1966468.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2201 a         1888 c         1559 g         2490 t
ORIGIN      
        1 ctaaaaattt ttgctacatc taaatagttt gacctcttcg gggttgcttt ggtcgttggg
       61 attgcgattg catctggtta cctcaactca cacctcgata aatttgtaga ctggggatca
      121 tggacagctc ttgtaccctt tggattgatt tccgtaacca acgttgggat ttccatgttg
      181 tccactcgtt tcacggggaa attaagcaaa tggggaaatt actttggtat tgttaatacc
      241 attttgtccg gtgctattga ttatatcctt ggaaataagg cggccatcat tacctatccc
      301 gtcaccttcc tcatttatac ctttgctata aagaagtggg aagcttcgca agaaggcaga
      361 cccaaccaaa tgagccaaaa acaggtaaaa ttggcggcca tcatcatttc catcatcgcc
      421 ttcctctttg cctttgtgac caactatatc ggatatgggg gcaagatgaa tctccttgcc
      481 tacgtaacaa ctattgcctt tgcactgtcc ctcattgcca atgctttgaa tgcattaaaa
      541 ctaacaactc agtggggctt ttggttgatt tacaatttcg ttcagctgac aaaagctggt
      601 attcaaggaa acttcgccaa tatcggtaaa tacatctttt atatcctcaa tgcaatcgga
      661 gctttatttg tctggaatga tgaagaagtt agataataga aggaaactca aataaagaat
      721 actagccaac ttaagagaat ctcccttctg ctatttttat cattttagtt gtaactccca
      781 ttcttttgga gtagaatgaa gttagataaa agagggaggt cttgtcatga aaaaactcta
      841 tagaatccat tttatagcaa ttgcagttat tgatttgcta ctatttgcat tttttatcac
      901 tagactcgag acatcttttg agtggctctt gctctctggt ttaattttct tccttgctca
      961 aggactcctg ctatttctct tggtcgtccg actcaaacat caattcgctg agatttatcc
     1021 tcaaatcaat aaaaagattc gcttctacta tttaggggtt ctcaccattg attttctatt
     1081 ttttgttctc ttagccttca ttagttctca gcgtttttca tctcttatgc caatcatcac
     1141 tgcttgccat tctacttttt attatatgac agctgactac ctaagagaaa actatccaga
     1201 cttttacgac aaacacatct ctttatggga gtgtctctaa ggaaaaggag gttttagcat
     1261 gaaaaaaatc atcttcatca aaaccattca actccttgtc attgatggaa tcatgctggc
     1321 atttttgaca tttaaaaggg ggcttacttg ggactggatt ttgatttata gcggttggct
     1381 cattttcttt catcctgtgc tattgaccta tctttcaaac caactttgtg accactttag
     1441 ttaactctat tcccagatta gaccgagatt ctggcgtttt gctttacaaa ttctcctatg
     1501 ggatagcctg atgattctct ccttggtgtc tttaagtgat attccacttt tccttcaggg
     1561 aactctcctc atcctaggac atctcatccc ttcctatcgc atctgccaaa gcctgaaaag
     1621 agacttcccc caagcatatc aagaaccgat ttctttttgg agtattttat gatagatgag
     1681 aaagaccaag ccgactgggc ttggtctttc ttatctcttt ttagtatcta ggataatggt
     1741 aacaggtcca ttattaacca gctcaacctg catatctgct ccaaagatgc ctgtctgaac
     1801 gggcacttct tgcgctaatt tttgattgaa agcatcatag aagtctgatg ccatatcagg
     1861 tttagctgcc cctgtaaagg ctggacgatt gcctctctta gtatccgcaa agagggtaaa
     1921 ctgagaaata gagaggattt ctccttcaat atctttgaca gacaggttca tcttgccttc
     1981 tgcgtctgaa aaaatccgca tattgaccag ttttctcaca gcatagtcca aatcttcctc
     2041 ttggtcctct ggtccaacac caaccagcaa taaaagtccc tgattgattt ttccctgaat
     2101 ctggccttct atactcactt gggctttttt aacccgttgg ataatgattt tcataatagc
     2161 ctttctagta agagctagga caactagccg ttggtccgtt tgacagagta aacttctggc
     2221 acactcttaa ttttatcgac aaccgtggtc agtgtagaga ggttggcaat accgaaggac
     2281 acatggatat tagcaaactt catatccttg gttggttggg cattgaccgt tgaaatattc
     2341 ttggttgtat ttgaaagaac ttgcagtaca tcgttcaaca gtcctgtacg gttgagaccg
     2401 tagatatcga tatgggccat atactcctta tttgagctag ggtactggtc ttcccattcc
     2461 acatcaagga gacgttgctc gtagttttct tgggcacgca ggttcataca gtccacacgg
     2521 tgaatagcca caccacgacc cttggtaatg tagccaacaa tatcgtcacc aggcacgggg
     2581 ttacaacact tagcaatccg cactaggaga ccagaagcac cttcaataac cactcccccc
     2641 tcatgcttga ccttgagggt ttctttattt tcaaccttga cctcgccacc tttgacaagc
     2701 tcctctgcct cagctttggc cttggcacgc tcttcctcac ggcgttcctt ttcagtcaga
     2761 cggttaaaga cggtaatcgc accgatttcc ccaaaaccaa tggccgcaaa gagggagtct
     2821 tctgtcttgt aactggtctt ttgcagaact tgatccatgt ggcgcttgtc cataaattta
     2881 tttgccacat agccattttc ttggaactga gccatcagca tctcacgacc cttgttgaca
     2941 gacaattcct tatcttggtt tttaaagaac tggcgaatct tattgcgcgc cttgctagtc
     3001 ttgaccatat tgagccagtc acggctaggt ccaaaggagt tcgggttggc gataatttca
     3061 acctgatccc ctgtctttaa cttggttgtc agtggaacca tgcggccatt gaccttggca
     3121 ccagttgctt tttcaccgac cttggtatgg atttcgtagg caaaatcaat cggtcctgaa
     3181 tctttgggaa gggaacggac agctccatct ggggtaaaaa cgtaaatctc ctcagccaaa
     3241 tagttttcct taacagagtc cacaaattcc ttagcatcat cagcctggtc ttggagctcc
     3301 atcatctcct tgatccagtt cattccaata gctgattcct tgctgttaac ttgccccttt
     3361 atacctttct tataagccca gtgagccgca accccgtact cagccacctc gtgcatttcc
     3421 ttggttcgaa tctggaattc aatcggccct tttggtccat aaacagtcgt atggatagac
     3481 tgataaccat tggccttgcg gttggcgata tagtctttga agcgacctgg catcggtttc
     3541 caaaattcat gcacgtaacc aagcatggca taaacatcac tttgggtatc taaaatacaa
     3601 cgaatagcaa tcagatcata gatttcctca aaccgttttc tcttgtcctg cattttgcgg
     3661 aaaattgagt aaatatgctt gggacgacca taaatcttcc ctttcaagtg acgttctgtc
     3721 gtatactcct ctaattttgt gactacctca tccaccaagg cctcacgctc cctgcgcttt
     3781 tccttcatca tatgggtaat cttgtaaaac tccgttggat tgagataacg gaaagacaag
     3841 tcttctaatt cccatttgac actggaaatc cccaaacgat gggcaagcgg ggcatagatt
     3901 tccatggttt ctttggaaat acgctcctgc ttgtcttttc gaagatgttt cagggtccgc
     3961 atattgtgca agcggtcaga cagtttgacc aaaataacgc ggatgtcctc agacatggcc
     4021 atgagcatct tgcgatgatt ttccgctaat tgctcctcga tcgatttgta ctcgaccttg
     4081 ccaagcttgg taactccgtc aacaatcatc cgcacatcag gaccaaactc tctttccaaa
     4141 tcgtccaaag tcgcatctgt atcttccacc acatcatgca agaatccaca agctactgtt
     4201 acagcatcca gctttagctt agctaaaata cctgccactt ggatagggtg aatgatataa
     4261 ggctcgcctg atttgcgata ttgaccactg tggcattcaa cagcatagac caaggcctta
     4321 tggacaaaat gaacatcctc ttccgttaaa tattctttgg ttaaagcgac aacttcttcg
     4381 cctgttaaat tcacttcttt cggcatctct actctccaat tcttcctacc attttatcac
     4441 ttttttaaga atatgaaaac tagattggaa cagaataaga aaaaaataat tcaaaattgc
     4501 ttgataattc tgaattattg gtccgtaata tactacgaag ttagatttta aacttaggtg
     4561 atagaaggag agatagaaga acggaaacca tattgtaacc caaagacttt ctgacttccc
     4621 caattccatt gaagatacga aagataaacg gtggaactcg tatcacatac actggtacct
     4681 tgactggatt ttggaattaa tactaaatga aaatcaaaga gcaaactagg aaactagccg
     4741 caggttactc aaagcaccgc tttgaggttg cagataaagt tgacgcggtt tgaagagatt
     4801 tttgaagagt ataaaaatcc tcaagatact ttcttctatc ctttagttta taaggagaat
     4861 acctatgaaa aaaactgcta tttctatctt tgctctccta atgttaggag tttgctgcct
     4921 gttcctattc agccagcaaa gctataaaaa acagtcgttc aatactatgc taacgaccag
     4981 aacctgccca gtaggataac ttatagtgaa tatagcgaca aatgagaagc caactacggt
     5041 agcactctaa acatcacgtc tatcaaacaa gctaatgacg gagtttatgc aacctatgaa
     5101 gggcaattga cacctttcca atattgataa attgataacc agcctgtctt catctagtca
     5161 tgctggtttt taagttcatt ttaaatcctt acctattctc cctaactgtg ctatacttaa
     5221 tttatactca atgaaaatca aagagcaaac tagaaagcta gccgcaggct gttcaaagca
     5281 ctgctttgag gttgcagata aagttgacgc ggtttgaaga gattttcgaa gagtattagt
     5341 acattctttg agattggagc tagtatgaaa atccataaaa ccgtgaatcc tgttgcctat
     5401 gaaaatacct attatctaga aggcgaaaag cacctcatcg tcgtcgatcc tggtagtcat
     5461 tgggaagcca ttcgtcagac aatcgagaag atcaacaaac cgatctgtgc tattctcttg
     5521 acccacgccc attatgacca tatcatgagt ctggacttgg ttcgcgagac gtttggcaat
     5581 cctcctgtct atatcgcaga gagcgaagcc agctggctct acactcctgt cgataatctc
     5641 tccggtctcc ctcgccacga tgatatggca gatgtggtca caaaacctgc agaacacacc
     5701 tttgtctttc acgaagaata ccaactagag gaatttcgtt ttaaggttct accgacccca
     5761 gggcactcta tcggtggtgt ttccctagtc tttcctgatg ctcatctagt cttgacggga
     5821 gatgctctat tccgcgaaac tatcggacgg accgaccttc cgactggtag catggagcaa
     5881 ctccttcata gtatccagac ccaactcttc accctaccaa actacgatgt ctatccagga
     5941 catggtccag ctactactat cgctcacgaa aaggccttca atcccttttt ctagcaagat
     6001 gatgacaatc gaaatttaag taaactatcc agcaaatctt tctattacaa aaggcatcct
     6061 atcaaggttt tcacacatga ttggatgcct tttttctgat gactagattt tttgcattac
     6121 caaataatca cgcgctcctc tggtgaacgc cacattccgt ctccttcttt gacatcatag
     6181 gttgtaaaga aatcgtcgaa gtttggtact tgcacattga cacggagttt ggctggtgcg
     6241 tgcacatcga cgctagccaa aagtttcata aattctggtc gacctttcat gcgccagatg
     6301 cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg cttctctctt agctgcttca
     6361 agcgctgctg cgattcctcc caagtcagcc acgttttctg atacagtcaa tttaccgtta
     6421 atggttgctc cataagaatc ctgtccatca aattggtcaa tgactttttg tgttttctcc
     6481 ttgaaggcag catagtcgct ctctgtccac caatccttga ggctaccatt ttcgtcaaag
     6541 gaagccccgt tagtatcaaa ggcgtgggaa atttcatggg caatcactgc cccaatacca
     6601 ccgtagttag cagaagatga ctgatgcaag tcatagaaag gcgcctgtaa aatggccgct
     6661 ggaaagacaa tcaggttctt ctgaggattg tagtaggcat tgaccatatg agcaggcatg
     6721 ccccattcct tataatctac aggctggttc cacttactcc aactgtgctt gatttccaca
     6781 cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt cattcactac cttatccttg
     6841 taacgtgcag gcaattcttc tggatagcca atataaggtt tgatcacatt gagcttcacg
     6901 atagcctgtt tacaggtttc tggagtgagc cagtcattct taagcagacg ctccttataa
     6961 acatcaatca tggttgccac ttttttctcc acatccgcct tggcttctgg agagaacttc
     7021 tcacgggcgt accaaagacc cagggcttgc ttgaaaggtt cttgtgctag atgataagct
     7081 gctttgacct tatcttttgc ctctggaact ccagaaaggg cacggctgta ggcaccagac
     7141 aaaacacgga tatcctctgt taaatagctg gttgaaagat tgacaacact caaaatcaag
     7201 gttgctttaa ggagagacca ggcttcctca ctgtagaatt gctctgctgc ttgccagaaa
     7261 cgttcctcgt ctacaataac cttgtctggt aattgcccaa taactgcttt gaagaagtca
     7321 tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt aagaatatgg atgatagagt
     7381 ttagcatatt ctgaactttc ttcattagag agcaccactg ccgcaactcg gcggtccaat
     7441 tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag agaaatcata agccttgagg
     7501 agatttgcgc tgctttcttt ccaaagagtc aagagctctt cgcgctgagg atgttcttct
     7561 gcatagtagg tcgtatctgg caagattgtg cttggagcgc tagcccatag aacattgatt
     7621 ctagcatcca taaagtctgg cgatacacca aaaggaagga agtttggttt tcctgcaagc
     7681 tcaaactctg ctagtttagc tgtaaaatcc gcaaaagtct ccaattcttg gaattcttta
     7741 aggagtggta agacaggtgt gataccgtca gcttctctct tgtcaaaatc acgaactagg
     7801 cggtggtatt tgacaaagtt ttccaagata gcatcctcag gcacttcttc acctgctaac
     7861 cacttgtctg ttgtcgccag catcaggtct tcaatttcct ggtctaaatc aacaaaacct
     7921 cctgtttgag acttatctgc tgggatttca gctgtctgtt gccattctcc attgatagca
     7981 tcataaaaat catcttgata acgtgtcatc ttgttctcgc tttcatttgt atttgccttt
     8041 atcttaacaa aaatcactag ggaatgcaat tagtaaccat ttaactatta ttttttagct
     8101 attataaaga atctttccat gataaaacgc ataattcc
//
LOCUS       DAVMEF010000086         8131 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMEF010000086 DAVMEF010000000
VERSION     DAVMEF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.086"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..186)
                     /locus_tag="VYQ30_001367"
                     /pseudo
     CDS             complement(1..186)
                     /locus_tag="VYQ30_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(232..465)
                     /locus_tag="VYQ30_001368"
     CDS             complement(232..465)
                     /locus_tag="VYQ30_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1966469.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            complement(471..1463)
                     /gene="plsX"
                     /locus_tag="VYQ30_001369"
     CDS             complement(471..1463)
                     /gene="plsX"
                     /locus_tag="VYQ30_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1966470.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1460..2230)
                     /gene="recO"
                     /locus_tag="VYQ30_001370"
     CDS             complement(1460..2230)
                     /gene="recO"
                     /locus_tag="VYQ30_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1966471.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2227..3396)
                     /locus_tag="VYQ30_001371"
     CDS             complement(2227..3396)
                     /locus_tag="VYQ30_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1966472.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3545..4555
                     /locus_tag="VYQ30_001372"
     CDS             3545..4555
                     /locus_tag="VYQ30_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1966473.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(4758..4838)
                     /locus_tag="VYQ30_001373"
                     /pseudo
     CDS             complement(4758..4838)
                     /locus_tag="VYQ30_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4873..5310)
                     /locus_tag="VYQ30_001374"
     CDS             complement(4873..5310)
                     /locus_tag="VYQ30_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1966474.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKSYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5395..8028)
                     /gene="polA"
                     /locus_tag="VYQ30_001375"
     CDS             complement(5395..8028)
                     /gene="polA"
                     /locus_tag="VYQ30_001375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1966475.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYVDYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2230 a         2090 c         1505 g         2306 t
ORIGIN      
        1 actttggcaa agatattctc aaccttgctt ctctccttag atagcgcatg attacaggct
       61 ttatcttcaa ctgttagcgg cttgagtttg ctggatttac gtggaagttt gtgcttgagg
      121 atatatcttc atgagccctt gataaccact gtcagccaag attttaccag cttgtccgat
      181 atttctcatt tctaaaaacc atctacttcc gttgacttgg aactccgatt gctattttcc
      241 ttgaatgatt ttaaccacat ctcctacact ttggagttgg tcaatttcct catcgctgat
      301 ttcgatacta aattcatctt ccagcgtcaa gataaactcc atcaaatcaa ctgagtcagc
      361 atccaagtcg tctttcagac tcaaggattc tgtcacgaca aagtcctctc cctgtcgctc
      421 ttggataatg gtcacaatac ggtcaaaaat ttctttttct gtcatctctt ttattctcct
      481 gaaaattcac gcgcagtctg ggcaaccacg tctgtttcta gcatggtacg gatctgacga
      541 atcgtactat aaacagcctt ggcatcgctt gagccatgag tcttgacaac aggtgcctta
      601 acaccaaaca agaccgctcc accaacatct gaataattga gctgtttttt caaaccactg
      661 aggctgtcct tgagaaggag ggcacctagt ttcgctcgaa gaccaccacc tgtaatagct
      721 gtcttgagca agcccatgat tcccatagct gtcccttcga tggatttgag cacagcgttt
      781 cccgtgaaac catctgccac aacaacatct gcaacgccat tcatcaaatc acgcgcttcc
      841 acgtttccga taaagttcaa actttcatca gccgccagta attcataagt ttccttacga
      901 agcgggtcgc ccttgctact ctctgttccg ttgttgagca aaccaacgcg tggttgcgca
      961 atgccacgga catttttagc atagaaggaa cctaggacag cgtattgatg gaggtgctgg
     1021 gctgtatttt ctgcattagc accgaggtca agcatgtcaa aacctttccc atctatagtc
     1081 ggcaatgttg acataagtcc aggacggtcg atattcttga tacgacccac gatgaagaat
     1141 ccagcagcca acaaagcacc tgtattccca gccgaaagga cagcgtctgc ttcaccatct
     1201 ttgacagcct tggctgccaa taccatactg gcatttttct tattccgaat agctctcgta
     1261 ggttcatcat ccgaatcaat cttctcatcc gtatggataa tgctgacgcg ctctgtcgct
     1321 gtcagatatt gcttgatttt agcttcatct ccgtagagtt gaacctcgat atctgaaaag
     1381 tcagataggg cttgattgac accctcaacg atggcctgag gtgcgtaatc gccccccatg
     1441 gcatctactg cgattttttt catttctttt cctcttttag taattgtccc cagtctgcta
     1501 gggaatcaat aaatttcttt gattttaggt gaatcccaac gtactcttca tatagttgat
     1561 ccataaattg gcgtagctct tgcttgattc caggcttgag cgaaatggtc tccaaagtct
     1621 caaaatcaat agcttgaaat tgattgagca gataggggat attgggattg agatggcaac
     1681 gtctctcatc ctcatgataa tgctctggac agaggcaggc tccatatttg aaagaaaagt
     1741 caaaagcctg accaacccga tggcagaaga cacactcatt aaaattgagg ctgattccaa
     1801 atcgagtcaa aatttgaatt tcaaaaatat tggtcaaaac ctgataatcc aagcctgctt
     1861 ccatcaactc caaagtcttt tgcaaaaaag caaacaaggg agcatcctgc tgattgtcct
     1921 gcaaactagc atctgcaaga gctgccacat aggtcgcata ggccatgaca aagaggtcac
     1981 tattaatctt gggaaaagtc atgacctcat gatagtcttc gatgtaactg agtccgtcat
     2041 cattgattcg caagagaaat cgtgccagca ccaagggctg aataacaggc gccagcttag
     2101 actgaccagc gtgtttgaca aaaaacatgc gtttgccaac ctgctctgta aaaattttga
     2161 cgagcttatc atcctcacga aaattgcgat tgtaaagcac caagccttga ctcgtgatag
     2221 actgaatcat gcttctctca tgtactcctc aagtcgtttc atggcttctt tgatagtctc
     2281 catgctggct gcataagata ggcggacgta gccttccccg taacgtccaa aggctgcacc
     2341 agggataaag gcaacggcct tcttctgagc aaaatccttc agaaaagcaa aggagtcttg
     2401 attgtagccc gctggaattt tagcaaaaat atagaaggca ccgtctggtt tgataatctc
     2461 aaaaccaaga gcagtcattt tttcgatgat atagtcccga cgttggatat attccttctt
     2521 catgggctcc gcatcgtttt taccagccgt caaggcttct accgcagcat gttgcgccat
     2581 ggtatttgcg gcagtgacca agtactggtg actcttgatt aactgggctg tgaaggtcgc
     2641 aggagcgaaa atcagcccca aacgccaacc tgtcatggca tgcgatttag acaaaccatt
     2701 gataataata gcctggtctc tcaacatcgt tcctagagac acatgggctt cgcccgtgta
     2761 ggtcaattct gagtaaacct catcacagac aacaaaaatt tcgtacttgc gtaaaacagc
     2821 tgccaaggcc tctaactgct ctcgactgta ggtaattcct gtcggattgg ctggatagtt
     2881 gagaataacc gccttgagct tatcaccctg ctccaaaatg gccttctcca acatctcagg
     2941 agtcaagaca aaaccatttt cagtcgtatc aatctcaaca atttctgccc caactagatt
     3001 gacaatcggt tcataacccg gataagcagg agctggcaag agcaccttgt ctccctcttc
     3061 caaaatagcc gtcaaagtcg cagataaagc ctctgtagct ccgatagtga ccaagatttc
     3121 attttcagga gcatagtcca gttggtactt ttccttaaca aaatcactgg ccgcctgacg
     3181 tagagtcagc agaccactca tccctgtata gtaggattgg ttctgatcaa tcgctcgctt
     3241 ggccgcctcc ttgacatggt ctggcgttgt aaaatcaggt tcccctaagg tcaaacgcaa
     3301 gaccccagga atctccgaaa tagcctggtc aaactgacga atcaacgaaa cttgaatttt
     3361 atctaactgt ttattaaagc gcttagttaa gtccatagat acctcctact ttcaaaacat
     3421 atctctatta tactacaaaa gccccccgac cgcaaaaata cattagagaa tagctttcca
     3481 ctttctattt tacttttcct gtttacaggt ctataatggt aacagattct atttggaggt
     3541 ttttatgtca tttctatcaa aaaatggagc aggcatcttg gtctgccttc tcatttctat
     3601 cgtatcttgg tacttaggag gattcttccc tgtgattggc gcgcccgttt ttgcgatttt
     3661 tatgggcatg ctcctacatc cctttctctc gtcttataaa caactggatg ctggattgac
     3721 ctttagttcc aagaaattgc tccagtatgc cgttatctta cttggttttg gtctcaatat
     3781 ctctcaagtc ttcgcagttg ggcaatcttc actccctgtc atcctttcca ccatttcaat
     3841 agccttgatt gttgcctacc tcttccagcg cttctttgcc ctggatacaa aactggctac
     3901 cttgattgga gtaggttctt ctatctgtgg gggttctgcc attgcagcga cagcgcctgt
     3961 tatccatgct aaggaaaagg aagtagccca agccatctcc gttatctttt tcttcaatgt
     4021 cttggctgcg ctcatctttc caaccctcgg cacctggctt catctatcca atgacggctt
     4081 cgccctcttt gcaggaactg cggtcaatga tacttcctct gtaaccgcca cagctagcgc
     4141 ttgggacagt ctttaccaga ccaataccct cgaatccgca accattgtca aactcacgcg
     4201 caccctagct atcatcccca tcactctctt tctctcctac tggcaaagtc gcgagcaaaa
     4261 aaataagcaa ggcttacaac tgaaaaaagt cttcccactt tttatccttc acttcatcct
     4321 tgcatcgctc ctaaccaccc ttctgacctc tctcggtgtg tctagtagtt tctttacccc
     4381 tctcaaacaa ctctctaaat tccttattgt catggccatg agtgctatcg gtctcaaaac
     4441 caatctggtc gctatggtca aatccagtgg aaaatccatt cttcttggag ccatctgctg
     4501 gattgccatc atcctcacca ctcttggtat gcagaccctt atcggcattt tctaatactc
     4561 ttcgaaaatc tcttcaaacc acgtcaacgt cgccttgccg tatatatgtt actgacttcg
     4621 tcagttctat ctgcaacctc aaaacggtgt tttgagctga cttcgtcagt tctatctgca
     4681 acctcaaaac ggtgttttga gctgacttcg tcagttctat ctgcaacctc aaaacggtgt
     4741 tttgagctga cttcgtcagt cgtatctaca acctcaaaac ggtgttttga gcagcccgcg
     4801 gctagtttcc tagtttgctc tttgattttc attgagtata acacaaaagg tagcccatca
     4861 gctacctttt tcttatgctt cctcaatcaa gcgagtatgt tctctcttga tacagcgatt
     4921 catcacgata tcatcacatc caccatcacg caaaatctct tttgcttcta aactttcaag
     4981 tcctagctgt gcccaaaaaa tcttggcatc agctttgaga aaatcacgcg ccacatcggg
     5041 cagaaattca ctgcgacgat aaacattgac aatatctaca ggaaaaggaa tttcagcgag
     5101 gctagcataa gacttttcac ccaagatttc gccacctgcc gccttgggat tgactgggat
     5161 gattttatag ccccgagcct gcatttcctt tgtcactcga ttgctggttg tttcttcacg
     5221 gtcagacaaa cccaccacag caagggtttt actcgttgcg agatactgac gaatcacgcc
     5281 atcacttgga ttgataaatt cttgactcat agaaatcctc ctttttcatc agtatagcac
     5341 attttgaaaa ggtttgcaga attctactac aaaaaaggag gactagcccc ctttttattt
     5401 agcctcgtac caggttgccc cttcattctc atctgcgata agaggaacac tgagttgaat
     5461 ggcttcttcc atggtttgtt tcaccaattt tttcatctct accaattcag atttaggcac
     5521 ttcaaggacg atttcatcgt gcacttgtaa cagcatctta gtctgataac cacctgcaac
     5581 caaggcttta tccagctgaa tcatggcaat cttgagaata tctgctgccg aaccctggat
     5641 aggtgagttg atagcagttc gctccgcaaa accacgaata ttgaagttgc gcgaattgat
     5701 atctggcaac tcacggcgac gcttaaagag ggtctctaca tagcccttat cacgcgcctc
     5761 ccgcaccact tcatccatgt agtttttaat acctggaaaa cgttcaaagt aggtatcaat
     5821 gtaggctttg gcttccttac gactaattcc caaattatta gacaagccaa agtctgaaat
     5881 cccataaacc actccaaagt taactgcctt ggcattgcga cggtcatttg cagtcacatt
     5941 atcaggacgc tcaatgccaa agacccgcat ggctgtcgaa gtatggatat ctgccccctc
     6001 ttggaaggcc ttaatcaagt gctcatcctt agaaatatgc gccaaaacgc gcaattcaat
     6061 ctgtgaatag tcagagctga gtagcacact atcctcccac tctggcacaa aagccttccg
     6121 aatcaagcgc ccttgttcca atcgggcagg aatattttgc aagtttggat ccacactaga
     6181 caaacgcccg gtctgggtca aatcctgcat atagcgagta tgaatctttc catcagccaa
     6241 aatccagtcc tgcaagccaa ttacataagt agattgaatc ttagcaattt gacggtaatc
     6301 caggattttc ttaacaatcg gagcaatagg agcgagacgc tccaacacat ccactgctgt
     6361 cgaataacct gtcttggttt tcttagtgta ttctagagga agtcccaatt tctcaaagag
     6421 aagcacgccc aactgcttag gcgagttgac attaaactcc tcaccagcca gctcgtaaat
     6481 ctcttgagtc agtttttcaa tgacaagctc attttcagcc tgcatctcaa gcaaggtctc
     6541 tttcttgacc ataatcccag caatttccat cttggcaagg acaaaagcca gaggttgctc
     6601 catatcataa agaagctcta attgcccatt ttcgctgagt ttttcaagta aaataggctc
     6661 tgtttctacc aaaacagcaa gtttacaagc taagtgttcc aagaatttct cacgttcagg
     6721 aatggccttt ttaacaccct taccgtagaa agtttcatca tcaaccaagt aagtctgacc
     6781 ataaagacta gcgatggtcg caatttcatt gtcctccaca gtcgaaagga ggtatttagc
     6841 caaacggatg tcaaaagcag gcgcctgcaa atccacacca aaacgttgca aaagaacttt
     6901 aaccttctta aagtcataaa ctctcagaga tgttttttct aagaaatcct tgaaaatcgg
     6961 gtcttgcaac agctcaagct tgtctgtggc atagagctta tccccacaag accaggcaaa
     7021 tccaaccaaa ttatccgtat ggtaattctc accaaaaagc tcaaagtgga agatagactc
     7081 ttcactcagc atatcttgac tgatttggtc aacaatagta aaatccaaac cctcagccac
     7141 atcagctgac gacacattta aagcctgctt gagctgtttg aatcccatct catcgtaaaa
     7201 tttcccaaga ttttcaacat ctggaccact atagaccaag tcctctaaac caatcgcaat
     7261 cggtgccttg gtatcaatgg tcgctagtgt tttagacaaa aaggcctgtt ccttgtcatt
     7321 aatgagattt tccttcatct tagaagtctt cattccatca atattttcat aaatcccctc
     7381 aagcgaacca tgctccagca agagcttaat acccgtcttt tcaccgactt tggtcacccc
     7441 agggatatta tccgacttat cacccatgag cgccttgaga tcgataaact gagctggtgt
     7501 gaggcccatt tcttccatga ggtaatctgg cgtaaaggcc tcaaactcag ccacaccttt
     7561 cttggaaatt tcaaccaccg tatgctcatc cgtcagctga atcaaatcct tgtccccact
     7621 gacaatggta atatcaaaac catcctgctc tgctagctta tccagcgtcc caatgatgtc
     7681 atccgcctca tactgagcca gctcatagtg acgaatcccc atatgatcca gcaactcacg
     7741 aatgaaagga aattgctcac gaaactcatc aggagtcttg gcccgaccac ccttatagtc
     7801 cacatacatc tctgtccgga aggtcgtctt tcccgcatca aaagccacca aaatatgact
     7861 cggctcaacc cgctccaaca aatgactcaa catcaactga aaaccataaa tcgcattggt
     7921 atgcaaacca gccgcattct taaaacggtc caactgctga tacagcgcaa aaaacgcccg
     7981 aaaagctaca gaagacccat caatcaataa taattttttc ttatccatac acccattata
     8041 aaggaaagaa tcaaaaaata ccattgggaa gagctagagc tagtattttt caaacttttt
     8101 ccgaataaat agatagagcc agagaattta g
//
LOCUS       DAVMEF010000087         8050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMEF010000087 DAVMEF010000000
VERSION     DAVMEF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.087"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(232..1644)
                     /gene="sufB"
                     /locus_tag="VYQ30_001376"
     CDS             complement(232..1644)
                     /gene="sufB"
                     /locus_tag="VYQ30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1966476.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1698..2138)
                     /locus_tag="VYQ30_001377"
     CDS             complement(1698..2138)
                     /locus_tag="VYQ30_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1966477.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(2125..3351)
                     /locus_tag="VYQ30_001378"
     CDS             complement(2125..3351)
                     /locus_tag="VYQ30_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1966478.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKVINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3362..4624)
                     /gene="sufD"
                     /locus_tag="VYQ30_001379"
     CDS             complement(3362..4624)
                     /gene="sufD"
                     /locus_tag="VYQ30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1966479.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4652..5422)
                     /gene="sufC"
                     /locus_tag="VYQ30_001380"
     CDS             complement(4652..5422)
                     /gene="sufC"
                     /locus_tag="VYQ30_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1966480.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5572..5766)
                     /locus_tag="VYQ30_001381"
     CDS             complement(5572..5766)
                     /locus_tag="VYQ30_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1966481.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5794..7449)
                     /gene="dnaX"
                     /locus_tag="VYQ30_001382"
     CDS             complement(5794..7449)
                     /gene="dnaX"
                     /locus_tag="VYQ30_001382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1966482.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7449..7946)
                     /locus_tag="VYQ30_001383"
     CDS             complement(7449..7946)
                     /locus_tag="VYQ30_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1966483.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
BASE COUNT         2327 a         1823 c         1503 g         2397 t
ORIGIN      
        1 ggatttaccc actacaaata ttatagagcc taaaaaatcg cgattttaaa ccgcgatttt
       61 ttgatacaag ttcatcttca aaatcaaaga acaaactagg aagctagccg caggttgctc
      121 aaaacactgt tttgaggttg tagatgaaac tgacgaagtc agtaaccata catacggtaa
      181 ggcgatgctg acgtggtttg aagagatttt cgaagagtat aaaatcaaat tttatccaac
      241 tgatccctcc atttcatagc taatcaagcg gttcagttca actgcgtatt ccattggaag
      301 ttcttttgta aagggttcta caaatcccat gacaatcatt tcagttgcct cagattctga
      361 caatccacgg ctcatgagat aatacaattg ctcttctgaa atcttagata ctttggcttc
      421 gtgttccaaa gccacttgcg agttgtgaat ttcattaaat ggaatagtat ctgatgctga
      481 caagtcatcc atgataatgg tatcacattc aatgtgggaa acagatttct tagagttctt
      541 gttaaaggtg acttgtccac ggtagtcaac ctttcctcca cctttagcga tggatttaga
      601 cacaatagac gagctggtat gtggagcatt gtgaatcatc ttagcacccg tgtcttggtg
      661 ttgccctgca ttagcaaagg cgatagagag catggtacca cgcgctcctt ctccatcaag
      721 gtaaacagat ggatatttca tagtcgtttt ggcacccaag tttccatcaa tccactcaac
      781 agtggcatcc ttttgagcct tagcacgctt tgttaccaag ttatagacgt tatcagacca
      841 gttttggata gttgtataac gcatataagc tccgtccaaa gcaaaaattt ctacaatggc
      901 agcgtgtaag ctattgcttg aatatgttgg tgctgtacat ccttctacgt agtggacgct
      961 tgctccctca tcaacgataa tcaaggtacg ttcgaactga cctatatttt cgttattgat
     1021 acggaaataa gtttgaagtg gaatatctac cttgacacct tttggcacgt agataaaagt
     1081 tccacccgac catactgctg agttgagggc tgccaacttg ttatctgtcg gcggtaccaa
     1141 cttcgcaaag tattgtttaa ataagtctgg gtattccttg agtgcggaat ctgtatctgt
     1201 aaagataata cctaattttt ggaactcttc cttcatgttg tggtaaacca cttctgactc
     1261 gtactgggca gaagcccctg ctaaataagc acgttcagct tctggaatcc cgatacgttc
     1321 aaaggtttct ttaatctttt caggtacatc atcccaagaa cgggctggtt tgtcagatgg
     1381 tttttggtag tagattaagt catcaaagtc aatctctgac aagtctgctc cccaagtttg
     1441 catgggcatt tttttgaagg tttcataaga cttcaaacgg aactccaaca tccactcagg
     1501 ctcaccctta gcagcagata attcacgaat aacaccttcg ttgagtcctt ttcctgtcga
     1561 taagacaggc tctacatcgt catggaaacc aaatttatat tcaccaaggt caattggttt
     1621 tggttctact ctttcttcag ccatagtatc ctttctttct tcatttctaa taattcataa
     1681 gacaaaagaa acttgtctta ctgtttttct tgattttcaa ttgttttctt aagggcattc
     1741 caagctaggg ttgcacactt gattctttga gggaatttgg caacacctga caagaatgcc
     1801 gcgtctccaa gttggccttg acgctcatct ttttgccctt gaaccatttc agaaaaaata
     1861 gtcgccagtt ctaaaatttc ttgtttggtt tttcctaaaa cggcatctgt catcatacta
     1921 gcagaagcag ttgaaatcgt gcatcctgaa tttagaaaag caatatcttc caaacggtcc
     1981 tctgcatcaa acttgacaga gaggttgatg acatccccac aagtcggatt gttgagactg
     2041 atttgctcag catcttctaa cttcccttgg tgatgtggat ttttcgaatg gtctgctacc
     2101 actgccatat aaaggctatc tagtttagaa agtgccattg aaaaactcct ttgtcttttg
     2161 tagggcatcg actagtttgt cgcaatctgc cttggtattg tagatataaa aacttgcacg
     2221 agctgttgct gggacttcca aatactgaag caagggttgc gcacaatggt gaccagcacg
     2281 aacagccact ccttcataat ccagagccgt cgcaagatcg tgaggatgga gatcacctag
     2341 gttaaaggca ataacacccg aacgttgagc caaatcctga gaaccgtaaa tggtcaatcc
     2401 ctcaattgcc tgcagttttg gatagacgta cgcaatcaat tcctgttcat gagcttcaac
     2461 ggcatccata ccaatctttt ccagataatc aactgcagtc gcaagtccaa tagctcctgc
     2521 catatttggc gttccagcct caaatttcca aggcaattcc ttccaactag caaattgctc
     2581 gtagacaaaa tcaatcatct cgccgccaaa ttctactgga gacatttgct caagatactt
     2641 ttctttgccg taaaggacac cgataccagt cggaccagcc atcttgtgac ccgaaaaggc
     2701 gaaaaagtcc agatccaagt cctggacatc aatcttcata tgaggtgtag attgagcacc
     2761 atccactacc ataattgccc caacttggtg ggctaattga gtgatttcct tgatcggatt
     2821 gaccacacca agaacattgg aggcatgagc tagggaaaca aatttaacct tatcagtcaa
     2881 tttagctcgc aaatcctcca tatccaaggc accgtcttta agatagacat agacaagctc
     2941 tgctccagtc tttcgacaag cttcctgcca tggaatgata ttagaatggt gttccattac
     3001 tgaaatcaag acctggtctc cctcagtgag aatttcctca gcaaagcgtg ctacccagtt
     3061 aaggctggtt gtcgttcctc tggtaaaaag aacttccttt gtagagcctg cattaataaa
     3121 cttacgaatg gtttcacgag cagcttcata agaagctgtc gctcgttccg ctaaggtatg
     3181 gacaccacgg tgaacattgg cattgtcctg ctcatagtag ctgttaataa ctttcagaac
     3241 tactagtggt ttttgtgtcg tcgcagcatt gtccagatag accagaggtt catcattgac
     3301 aatctgatct aaaattggaa aatccttgcg aatcgcttct acatctaaca taggcttccc
     3361 cttagcgttt tgacaatttc tcttcgatag ttgcaatcat ttcatcacga acttccttga
     3421 ctggaatctc cacgataaca gatccaagga aaccacgaac aaccaaacgc tctgcagttg
     3481 ccttatccaa tccacgactc atgaggtaat acatgtcttc tggatcaact tgtccgatag
     3541 acgctgcgtg tcctgcagtg acatcatttt catcaatcaa aagaattggg ttagcatctg
     3601 aacgcgcttg gtctgaaagc atgagaacac ggctctcttg ttgggcatct gctcccttag
     3661 cacccttgat gatgtggccg ataccattga aagtcaaagt tgctttttca aggataaccc
     3721 catgttgtag gatatttccg atagagttgc agccatagtt agttacacga gtatcaatcc
     3781 cttgtacctg acgaccactt gaaagagcta caaccttcag gtcagcatgg ctaccattac
     3841 caatcaagtc actatcaaaa tcagcaacga catttccttc gttcatgaca ccgatagccc
     3901 agtcaatact tgcatcgttg cctaatttac cacgacggct aatgtaggca gtgacgtttt
     3961 cacctagtcg gtcgatagca gcaaacttga cttgcgcacc tgaacgtgca atcacttcta
     4021 ctgtgatatt agcagttgct ttggcacttc cttcaccgcg tgactctaaa cgctccagat
     4081 aattaatctt agaattttta ccagcgataa tcataatatg cttgttaaac ggcacattgc
     4141 tatcgctatc ttggtagaaa attccttcaa ttggctctgt gatttctacg ttatctggaa
     4201 tatagagtac agcaccactg ttaaagtaag ctgtgtggta agccgccaac ttgtcatcat
     4261 catacttaac agatgacatg aagaattctt cgatcagctc tggaatttct tctaaagctg
     4321 agtgaaagtc tgtgaagaca acaccctgtt cagctaactc aactggagtt tgttcgaaaa
     4381 cagtttgagt tcctacttgc accaacttca agtgatgatc taaagctgtg aaatctggaa
     4441 catttgctga tggctcattt tctgtaatcg ttccatcacc cagattccaa cggtggaatt
     4501 tgacacgctc aataactggt aattccaaag tctcaatctt gtcaaaagct ttttgacgga
     4561 gatcagccaa ccagcttggt tcagcatgca tttctgaaaa aagtttaata ttttctctag
     4621 tcatttactt ctcctaaaag atacgaggga attacaattc ttccttgtag tcgtagccaa
     4681 gttcttcagc taattttgcg tatccttcac gttccaaacg cgcagccaat tctggaccac
     4741 cagaaaggac aacacgacct tccatcatca cgtgtaccac atcaggtgtg atatagttca
     4801 aaagacgttg gtagtgagtg atgatcatag caccaaaacc ttcaccacgc atggcattga
     4861 cacctttaga cacaacttta agagcgtcaa tatcaagacc tgagtcaatc tcgtccaaaa
     4921 gagcaaatgt tggctccaac atcaaaagtt gaagaatttc attgcgtttt ttctcaccac
     4981 cagagaagcc ttcgttgagg taacgctctg ccatttcttc tttcatgttg agcaattcca
     5041 ttttttcatc tagcttagta ataaactcac gaactgaaat cttctcatca tcttctttac
     5101 ccgcattcat agcggcacga agaaactcag cattggtaat tccagggatt tctgatgggt
     5161 attgcatagc aaggaaaagt cccatacgcg cacgctcatc cacttccaac tcaaggatgt
     5221 ttacgccatc aaacaaaact tcacctttag ttacttcata gtttggattt cccatgatag
     5281 cggcagaaag agtcgattta cctgtaccat ttggtcccat gatagcggca atttctcctg
     5341 ttttcagggt caggttaacc ccttttaaaa tttcttttcc ttcaatctca acgtgaagat
     5401 ctttgatctc tattactgac atgatagttc ctttcttttt caaaactttt acagtactta
     5461 ctagaaaaac gtctgatttc cccagaaata gagcaaaaat tctataaata tacttttcta
     5521 gtataacaaa aaaaagccta aaaggctttg attttgtctg gaagctgagg gctaatcttt
     5581 cctattaaaa tgctgatcga tgacatcaac gattttaccc atcacataac tgactcggaa
     5641 atttctaaag agtaaaattg cccaaaaggc tgccataata tagcggccag tcatccaggc
     5701 ttcattgaaa aaataggtct cagcagctgt aaatagcgcc ataataataa attgttgttt
     5761 attcataaac ttattgtatc atgaaatctt tctttagtct tcctctacct tcactttatc
     5821 agccaaaaat tcaaatcctt ctggaatcag gctttcttct acttcttttt cagtttgaga
     5881 agatttggct ttggctgaaa aggctgcgcg aacttctttc cattcctcca tggaaatagc
     5941 taaaatctca ggtgaaaaac ctgccgcctg actgaggata ttaccaaaca tggtattgag
     6001 attgtctcgt ttcatagttt gaccagcatt gaagttagac tcaaaagcaa gaatagcatg
     6061 gtgttcattg gcagcaaccg gttgagaacc aactagcaga gccttgtccg gcccacctag
     6121 actttcaatt acctctcccc aggcattctg caaacgaatt agattttgac gtgctaaatc
     6181 aggattttcg acggcctctt gtaagataga ttgcacttta ttgcgatcga cacgatagac
     6241 tgttttgccc gtagctggtc gactaggagc tggtgcaact tgtttaggaa ccgcacctgc
     6301 attagaaagc tcttgtttga gacgggcaac ttcctgtctc agcgtagcaa tttcattttc
     6361 aaccgctcct gatagagctg gttcgggctt gatttccgcc aaacggacgg tcatcatttc
     6421 agcataaatc ttgggctgca aactagactt aatatctgct aaactcactg ttgctaagcg
     6481 aatcatttca aacagatttt tttgaggaag tgccaaattt tctacaaaga ctgaactatg
     6541 atgagtattt tctccccctg tttgaacaat taacaagtct cttaaatagt gcaaaagatc
     6601 ggtcacaaaa cgagtcatgc tcttaccatt gtcaaaaaga agattcaagc aagacaaagc
     6661 tttgggaaca tcctgttgag acaaggccgc cacataatca tccaaggctg ataggctaat
     6721 ggtgccagta atttcttcag agatagcagt cgtcagctca tttccctgtg tcaaactcag
     6781 ggcttgatcc aaaatagaca aggcgtcccg cattccacct tccgcccgtc tggcaatgat
     6841 ttccacagcc tctggttcag aactgatatt ttctttttct aagatatagt gaatatgttc
     6901 cttaatatcc tgtgtcttaa ttgatttaaa ctcaaaacgt tgcacacggg atagaatagt
     6961 agcaggaatc ttgtgcaatt cagtagtggc caaaataaag actacattct gtgttggttc
     7021 ttccagcgtc tttaggaggg cattaaaagc ccctgtagac agcatgtgaa cctcatctat
     7081 gatataaacc ttataacgag caaggctagg cgcataggta gatttatcac gaatttcgcg
     7141 aatttcatct accccattat tagaagctgc atccatttca atgacatctt ctaaactacc
     7201 gtccgtcact gcttgacaaa tatagcagtt attgcaaggt tcgccaccca cttgattggg
     7261 acagttcata gccttggcaa agattttagc aacactggtt tttcccgttc cacgaggacc
     7321 agaaaaaaga taagcgtgac ttattttctc ttgctccacc gcttgtttaa gagtcttagc
     7381 cacaacttct tgaccaacta actgggagaa gttttgactt ctatattttc gataaagtgc
     7441 ttgatacatt aagatttttc ctcaaacatc gtaaagtccc atgctgtctt ttcaagcaaa
     7501 atagcgacaa attgttccaa ataatctcga tccatagcat cgtaatcctc aatctctgaa
     7561 gaatccagat ccagaactcc aagtaactga ccattcttca tcatcggcac cacaatttca
     7621 cttttagcta gactatcaca agaaatatag ttgagatagg tcgtcacatc tccaacaata
     7681 acagtttcct gaaagtgagc tgcctcacca caaacaccct tgcctagtgc aatacggatg
     7741 caggaaacac ctccttggaa gggacctaaa accaattcct ttccatcgaa caaataaaag
     7801 cctgcaaata cggtattagg aaaacgtgat tttatgagag cactggcgtt ggaaagatta
     7861 gccaaaacat tggtttcgcc ttccaatagg aaggacaatt cttcatttaa catttgataa
     7921 cgtgattgtt tttctgattt taacataaaa ctattatatc aaaaaaggag cagagacaaa
     7981 agaaaaatcc cttgtttagt aaacaaagga ttttgggctc tataatattt gtagtgggta
     8041 aatcccctat
//
LOCUS       DAVMEF010000088         7669 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMEF010000088 DAVMEF010000000
VERSION     DAVMEF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.088"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(81..347)
                     /locus_tag="VYQ30_001384"
                     /pseudo
     CDS             complement(81..347)
                     /locus_tag="VYQ30_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(372..683)
                     /locus_tag="VYQ30_001385"
     CDS             complement(372..683)
                     /locus_tag="VYQ30_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1966484.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWETVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(608..849)
                     /locus_tag="VYQ30_001386"
                     /pseudo
     CDS             complement(608..849)
                     /locus_tag="VYQ30_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(850..1482)
                     /locus_tag="VYQ30_001387"
     CDS             complement(850..1482)
                     /locus_tag="VYQ30_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966485.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(1496..2491)
                     /locus_tag="VYQ30_001388"
     CDS             complement(1496..2491)
                     /locus_tag="VYQ30_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1966486.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2488..3186)
                     /locus_tag="VYQ30_001389"
     CDS             complement(2488..3186)
                     /locus_tag="VYQ30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1966487.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQINLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKYYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(3264..4274)
                     /locus_tag="VYQ30_001390"
     CDS             complement(3264..4274)
                     /locus_tag="VYQ30_001390"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1966488.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(4258..5265)
                     /locus_tag="VYQ30_001391"
     CDS             complement(4258..5265)
                     /locus_tag="VYQ30_001391"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1966489.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(5252..6205)
                     /gene="mvaD"
                     /locus_tag="VYQ30_001392"
     CDS             complement(5252..6205)
                     /gene="mvaD"
                     /locus_tag="VYQ30_001392"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1966490.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(6187..7065)
                     /gene="mvk"
                     /locus_tag="VYQ30_001393"
     CDS             complement(6187..7065)
                     /gene="mvk"
                     /locus_tag="VYQ30_001393"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1966491.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(7187..7516)
                     /locus_tag="VYQ30_001394"
     CDS             complement(7187..7516)
                     /locus_tag="VYQ30_001394"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966492.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TSCQWRMLPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2193 a         1821 c         1439 g         2216 t
ORIGIN      
        1 tctaccacct tctttaaata ccctccatta tcacataaac aggtaaactt ttcaattaat
       61 gactgcgctt ttcaatcacg ctagaggtac ttacttgctt ctttgatact aagttcagcc
      121 attctttcct tgtttttctc aataaagcat gttacccaag tgggattcgt tttggagtag
      181 tctcgcagag tccagccaat ggctttattg ataaaaaatt ctgtttggtt caagttatga
      241 aggagaatct tttccattaa ttgagtattg gtcttctctt ttcttaacaa ctggtggtca
      301 atagcgacac gtctcagcca gatattatct gataggctcc attttatact caatgaaaat
      361 caaagagcaa actaggaagc tagccgcaga ttgctcaaaa cactgttttg aggttgcaga
      421 tagagctgac gtggtttgaa gagattttcg aagaatatta agattatttc ttctagttca
      481 gggtgtttat acaccaaact ccctactact cgatctagga tatctaccgt ctcccaccag
      541 gattttgtca cgactaactg ctctagctta ggcaaatcgg tttcctttag ataagactgc
      601 attgctttca aatagttagc agccacatat tggtattttc taggctcctt ttcccagcaa
      661 gtgtctgcaa aatcccaatc gataatcttt gtttttttcg cttctggaaa atatttttat
      721 agagtttatt tctttcaggc accgcaatac ctagaaaaga aaattgatgg cgcatatagg
      781 cttccatggg ccttgctttt tagagtcttt tgctgcttct agctcctcaa gtaaatctgc
      841 taaactcatc taaaactcct cttgccccac caaatggtgc tgaaaggcat agacagccgc
      901 ctgagtacga tcgctgactt caagtttggc aagaatgttg gacacgtggg tcttgaccgt
      961 cttgagagag ataaagaggt catctgcgat gcgctgattt tcgtagccct tggcgatgag
     1021 ttggagaaca tctcgctcac gcgcagtcaa ttcttcatga agttccatat gattgcggtg
     1081 gtattcaacc ttcttgctaa cctcttgctc aatggccagc tcgccagcag ctaccttact
     1141 gacggcatga agcaattcat ctgcactaga agtcttgagc atatagcctt tggcaccagc
     1201 atctaagact ggcatgattt tttcattgtc caaataagag gtcacaatca aaatcttggc
     1261 ttcaggccat tctttaagga ttgctaaggt cgcgtcaatc ccattcatct caggcatgac
     1321 aatatccatg acaatgacat ctggacgcag ttccaaggcc aagtcaatcc cttgagaccc
     1381 gttggacgcc tcacccacaa cttctacatc gtcttggagg tcaaagtagc ttttcaagcc
     1441 caaacggacc atttcatggt catctactag taaaattttc atctttactc ctttatcatt
     1501 ccttatctaa caggggaata cggatatcaa ctgccaaccc ttgcttggga gctgttaata
     1561 actgaaccgt ccctgccata tcttcaaccc gctccttgat atttcgcagt ccataactca
     1621 agtcgtctaa gctccctaac tggaaaccaa tcccattgtc caccaccttc agttgcaatt
     1681 caacatctgt ctgatagagg tagacatcta ggcaagatgc ctgggcatgg cggagcgtat
     1741 tgctaatcaa ctcttgcagg atacggaaga tatgctcctc gattttctta ggcaatttcg
     1801 tcatattctg cttgagacta accctaagat cactcttgtc ctcaagctct tttaagagaa
     1861 tttgaatccc ttctatcaag ctcttctgct ccagttcaac tggtcgcaaa tgcaagagca
     1921 aaacccgcaa atccttctgg gctgtttcta aaatagctgt gacactctgc aactgggttt
     1981 gcatcttttc tctatccaat ttcaaagcct gctgactgat acccgataaa atcatgtggg
     2041 ccgcaaacaa ctcctgactg actgtatcgt gcaaatcccg agcaattcgc ttccgttcct
     2101 tctcgatgat ttcctcttcc tgagcaaggc tatgattttc agctttttga agagcttctg
     2161 tcaaaaggtt aagtttacct gataaggact tgaaactggc atccaaatct ggatctgcaa
     2221 cctgaaccac ttcttgccct gccaataaac gcttgagatt agcctgcatt tttcttagag
     2281 aaagctcttc gatccctcgc caaaacaggg ctaagagaca ggttatggac atgctgaaaa
     2341 ccaacaataa aaagacaaat ttttctgttt tttcgacatc gtgcaaaaag atagaccagt
     2401 caaaatcaag tatttccagc aagctgtggg agaaaaaaaa gacaaacagg aaggaggtga
     2461 gagcaataat gacataggct tgttttttca tcctctaacc acctccacat caccaatcat
     2521 agtggtcaag aaaatcttga cactcttgtt actcttgaga tagtattttg tttcttgatg
     2581 atagtgttca ttgcggaggg ctcgcttggg ctggttgaaa aaagtcaaat ccccatagag
     2641 acagttaacg ctgagactga cttccacatc tacaggtacg atgattttgg tcgttcctac
     2701 catctttctg aggataatga cattgtcatg attggttaag atgaccctct ccagatgaat
     2761 agtgtccttg cccatgaagc gaaagagatt gatatcatcg aattggcaag tctggtagct
     2821 tgaaaaatga tgaagatttc caaaccaacg atttttctcc ttcttaaccg tcacgacctc
     2881 ttcaaaaacc aaattgatct gctctttttc ctggttcatc atcgggtaaa gaagaaagag
     2941 gctatagata accgcaacaa aaatagctag aatcacaaaa ggattgagca taacgatgaa
     3001 aaagaagaga atggttgccg ctactaaaag aagattattt ccctctttac cagtgtagta
     3061 gcgaatcaaa agcaaaaaga ggaatagtat cagcagaaaa cgcgaaaaat gctctgatac
     3121 catcaaaatc agagctcctg tcagaagaca ggcttcgata aataaaaaga ttttaaattt
     3181 tctcataggt tcatcctctc ccttctattt tatcacaatt caaaaaagtc acctcagtct
     3241 gaggatggaa aaaaaggcgc tggttacgcc tttttcatct gatcatttgc ttcttttaat
     3301 tttccataaa gaagatagtc tactttttgt agatctgcta tggtggcaca gttaagggaa
     3361 cacataatca agcgtagatc tgctttccag ccttgaacaa tgccaatcac ttcttcaact
     3421 gtgtaggttt caaccaattc cagaacggtt cgtgacaatc ccacagactt agcaccaaaa
     3481 accaagcact taatcatatc cagcggattc cgaacccctc cactaaccaa gagttcgacc
     3541 ttatctttcc attcttgggc attgagaagg gcctgcatgg tagactgacc ccattgattg
     3601 aggtaatcac gctggccact acgacggttt tcgatatagg caaagctggt gccaccacga
     3661 cccgataggt ccactgtacg aacaccgaat tcataggctc tttcgattgt cttggcatcc
     3721 attccaaagc ccacttcctt gaggacaata ggaacgggaa tttgcttgct ataatctgct
     3781 agatgcgatt gccagcttct aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg
     3841 acattgacat gcacttgcaa tagaacagga ttcatctctt ctacagtctg aagtcctaac
     3901 tcgacaggct tgtccaatcc aatattggtt ccaaggagga gattgggatg actagacttg
     3961 acagaaaaag aatcatccgt tggatttttg agggctgcgc tataagaacc cgttacaaat
     4021 aaaataccac aggattccgc cacctgagcc agcttttgat tgatttctct tcccttatta
     4081 cttccaccag tcatggcatt gatataaaaa ggaaagtccc actttcgacc agcaaactct
     4141 gtcgaaagat cgatttcatc cagattgtaa agaggcaagg aagaatgaat cagctccacc
     4201 tcatcaaagc tattatagga acttttctgc tcaagggcat agaggatatg ctcgtcctta
     4261 cgatttgtcg tcatgtccta tcctttcttg atataagagc tcaatcccca gatcggccca
     4321 acgatttttt aaggttttgg ttgattgcgc atcaaaactc agggcgatgc cacagtcacc
     4381 accaccagca ccactactct tggcaacggc ctgcaaatct tgactggctt ctttcaactg
     4441 tctaagcaaa ggcgtgtaaa tatctgtact caagccttct aaaagcttgc tggctacttc
     4501 tacttgctcg ataacttttt cggctttccc ctgctccaag gcttcgacca aagaaaccac
     4561 cgtttctttt gaggaactta aaaaattttg attgatattt tgcttgattt gctggaccat
     4621 gtgactcgat acagccactt ccttggtcca tcccactaag aaatcacatt ctaaagttgg
     4681 tttcacttgt gagataaaaa atccccaatc acgctccaga actgtcgcca agttttcttc
     4741 ttctaaccaa gcagccgcct tctggcgatc aaatgactgg tagagaacca aatcctctgc
     4801 cacaatacag gcaaggtcgc ccatggaacc attgtctcct cgcttgagca agacagcgct
     4861 agtcagcttg aacaagagat tctgatcaac cgaaagatta tagagagcca gtaaagcctt
     4921 gacaaccaag acaacgacgc tgccactaga acctagacca aactttttcc cttctcgttc
     4981 cattttgcca cagattttta gggaaaaagg tcttaaattc tgaccgcgaa cagcgaggaa
     5041 gtctcccatc aaagcaatcg tttcttgaat caagctgtag tcaggattag gccttaagtc
     5101 cactgcgaaa tcaaacatat ctgaatagat acggtagctg tcagaaaaag caatctcagc
     5161 cctcatatag atgggaatat cctttatcaa agctaactgc cctggctcta aaatagcata
     5221 ttcacctgcc caatagagtt ttccgcaagt tttaacagca atcatcttga ctcaaatcct
     5281 ttgtttttga cacaatcaag cgataacgat gaccgaaaat ttctgataaa tgctccaagt
     5341 ctttctcctg acagaagacc ttaacattgg gaccagcatc catggtaaag tagcaggcct
     5401 ctcctttctc acgaagctgg cgaacaaagt ccatagcctc ataagaggca tccgtcagat
     5461 aagaaaaggc tggactagca gtctttgtcg tagcatgcat agccagggca tttttctccg
     5521 ttaattctcc aatcttggca aaatcatttt ccttgagata aatcagcata tcctgatagt
     5581 ccttctcaga ctgacgaacc cagtcgtcga aagtcgtcga ggtttccaca caaagtttca
     5641 tcccgtcacg gctagagatt ggttttttct tgtcctctag caccaacata atcatagcta
     5701 gtttcaagtc tgtctctaca gggtaaattt ctccactatc cttatcccag gctcctagtg
     5761 gtccataaaa actccgagaa gaagagcctg aggcaaactt ggcttcctgc gctaactgac
     5821 ttctatccaa tccaagcttg aaataagcat tacaagcctt gaccagggcg gacaaaccac
     5881 tagaacttga ggacaggccc gctgccgtag gcatattgtt ttgagtatcg atacggacaa
     5941 agccctcacc agctggacga taacggtcaa taatcttact catcttggca tgctcgacct
     6001 cattttgtag ctgaccattg atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg
     6061 acaaggtcgt ctctgtatac atattttcca aagttagaga aatactgcta gtagcaggca
     6121 ccatctcttt ttcttttttc tttccccaat atttaataat agcaatattt gcgtaggaac
     6181 gtactgttac aggctctcta tccatgtctg aacagctcct ttctcttcta atctttctgc
     6241 tagttcttgt gcgtgtgtca aattggttac caaggctatg atacaacctc ctagcccacc
     6301 accgctcatc ttggcaccca gagcaccatg gctaagagtc gtttcaacca aaaagtctgc
     6361 ctcagggcta ctgactccaa tttcttttaa atgtaaatgc gcttgactga ggatttgtcc
     6421 cagtccttca gcatcttttt gtgaaattgc aacttctgct tgctgggtta attctcccaa
     6481 ggcatgcaaa aacggtaggg catccttgcc cttattttga accacttgga tggcttcacg
     6541 agtatgacca taaacacccg tatcggcaat caccaaatag gcggataaat ccatctcaag
     6601 ttctgtaaat cctacgttct tgataaagcg aataggttgg tcactgagac aggtcttagc
     6661 atccaaacca ctaggattca tatgggcaat catttcagct cgattgacca agatttctag
     6721 tacatcatga ggcagatcag cctgatagta gtcaaatacc gcacgaatgg ccgctatgct
     6781 gatagccgct gacgaaccca tcccccgttt ctcagggata gccgagtcaa tctcacaacg
     6841 aatgcaggct tctgtgatat ccaaatactc cagtgaggca taaaccgcca tggacaaggt
     6901 atcctcctca taaaggcgcc aaggactctc tgcaggaact accttacagg tcacctccac
     6961 ctccaaaaga ggcagggaaa tggcaggata accgtaaacg accgcatgtt cccctattaa
     7021 aattatctta ctatgtgcct gaccgacacc aacttttttt gtcatttttt ccttttacta
     7081 gacgaaaaaa cgtcttattt ttcatacaag tattaattct ttcctatcta ttttattata
     7141 ttttcacaaa aaaagcgatt gtttccattc acaatcgctt ctttcattat tgaacccatt
     7201 cgccattata gttgacagaa tagccatcta cagtcgtatt cactgccaag gcacctgagc
     7261 tataagcgta gtaccatctg ccattgacct ggaaccaacc tgtcgtcata gaacgacgaa
     7321 agaaactcca taccgttaag taaagaggaa agtcgtgagg gagcatgcgc cattgacaac
     7381 ttgttttagt gacgtacaaa gtctcattaa caagtactcg tttcggccat ttataggtgc
     7441 ggtgtttgga gaaatagggt tcaatcttag cccattcttg atcgtttaaa tcagtatcat
     7501 atgctttgcg tgtcataact ctagtttaac atttttttgt gaatacaggt tctaaataat
     7561 cgaccacgaa aatttcttaa gtggaaaacg ccttatgaag tatgctacgg gaaagttatg
     7621 cacttaattt gacaattcaa gatgtaaaaa tatatactat attagattg
//
LOCUS       DAVMEF010000089         7661 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMEF010000089 DAVMEF010000000
VERSION     DAVMEF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.089"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(757..1956)
                     /gene="divIB"
                     /locus_tag="VYQ30_001395"
     CDS             complement(757..1956)
                     /gene="divIB"
                     /locus_tag="VYQ30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1966493.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(1966..3024)
                     /locus_tag="VYQ30_001396"
     CDS             complement(1966..3024)
                     /locus_tag="VYQ30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1966494.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVSPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(3028..4380)
                     /locus_tag="VYQ30_001397"
     CDS             complement(3028..4380)
                     /locus_tag="VYQ30_001397"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1966495.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVIPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(4484..5125)
                     /locus_tag="VYQ30_001398"
     CDS             complement(4484..5125)
                     /locus_tag="VYQ30_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966496.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(5127..7148)
                     /locus_tag="VYQ30_001399"
     CDS             complement(5127..7148)
                     /locus_tag="VYQ30_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1966497.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLIDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(7219..7518)
                     /locus_tag="VYQ30_001400"
     CDS             complement(7219..7518)
                     /locus_tag="VYQ30_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1966498.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYACIWTKWGEQVAFYCDYD"
BASE COUNT         2294 a         1667 c         1318 g         2382 t
ORIGIN      
        1 ttcttcccta ctaacactaa taagtccgat atggtaaatt cttgacctat tagttgctct
       61 aaccattttt ttcctgtttc ccaactaata catgtttctt actggatggt atatataaat
      121 gatagtgtcc ctcataagga gtaggtaata tcttaatctt atcaacaatt ctcataacaa
      181 acctaacctt taataaataa aagaggggtt aagtaaaaca tactctagtc cctcagtccg
      241 caatcaataa aataatgact aattctatta atctcttgct gcaagactaa tacggaaaat
      301 ttaaatgact gtctaaaatg aaattgaaaa agcttgatgt tagtaaatga acaaacttca
      361 acaaccagca agagcaagga ttgctaaacc aaatccgata cctgttgcaa gtccagctga
      421 aaaatcacta actggtactg aaatttcttc aatttcctca aataaaaagt tatcttgcat
      481 gattatcact ccctatcttt ttaacaacag gctactccaa ttaagagaac taccccagtt
      541 ccaagtaaaa cttcccccaa aaattcacca tctgtagggg ctacgatttc ttcaatttct
      601 tcaaataaga atacttcatc tgacatatgt gttaccttct tcatcaaaaa ttattttgta
      661 atcgattaca ttgcagatgt aacataaaga aaaacagatg tcaaatatta aacgtaaaaa
      721 catggtcact aaagaactat aagagaaaag gtaaacctag cgacgcgatg aacgctgggt
      781 cgtttggttt cgattgctct cttcctcttg ttttttctgt tcttcttctt gttttttctc
      841 agcttccttg gcctcttgtt tggctttttc ctcagcttcc ataattaatt tatccgccac
      901 agtgtagctg tagattccag cttccatgtc gaccacactc ggttctgaca attgaggctt
      961 aatcttactg taatatggca gtttcttact catttcagat agaggaacca agacttcgtc
     1021 cgaatcattc atggtcaatc gaattaaatc ggatgtcacc ttgcttgggg ctaattccac
     1081 cttttggata gccgccttga gttctgggct aatttgagca agttctgaga caaaaacctt
     1141 gatttgttca ctatcattaa agagaactga taaataagtt tctggtaaac tgttcagact
     1201 cacagaacta gtctcaagct gaccactgga aagaatagga taatgatttt caccagaaat
     1261 atagtaggcc acaatatcat attccttgac cttaatagtg aacttagttg gaaattgata
     1321 gacaagttga gctgattcaa cccaatagtt agacttaatc tgcttttcat attttgcctt
     1381 gtctagcaga aggttaatcg tataatccga atcctgaatg cctgaagcct gtcgaatatc
     1441 atcagctgta gtttgcaccg ttccctcaac acgaatatct ttcatggtcg cataaggact
     1501 gagcaagtag gcagagacaa tcaataaaag cagacttgga aataaaatcg tgaaggctcg
     1561 caagatatgg ataccaggaa tctttgcttt ggctggtttt tcctttgtag cctttttagc
     1621 aagcttttta tcctgttcct ccttctcttt agactctggt tcttctttct cttctttctc
     1681 tttgtcagcc tctgaggatg ctactttttc ttcagactct tccttagctg attctgaatc
     1741 tttctggtct gtttcactct cctggtcctg tttatcctct gacttctcag attcttctcc
     1801 cattcgagct tgtctttcct tttccttctc ctcagctaga gccgcctctt cttcagcctt
     1861 cttttttaga tattcttggt ttcgtttctg ccattctgat aactctttca attcttcgag
     1921 ggtttctttg tcctcatttt tcttatcttt tgacatttac tttccttatg ataaatcttt
     1981 tttcaacaat tgataaaaat ctgctagaga tttcaattcc ttagaagctt tcatcttagc
     2041 ttggtaatct tccttgtgac ttagtaagtg agaaagcttc tcttccaaac tatccaaggt
     2101 caaatcgctt tcttgaaggt cttctgcata gcctttctta acaaagtaag ctgcattttc
     2161 aatctggtca ccacgactag cttcacgacc aagcggcaca atgacatgca attttgctat
     2221 cgccaagagc tcaaaaatcg tattggcacc acctcgtgtc acaacaatat cagccaattc
     2281 catcaagggt tgatagagat cggtcacata gtcaacacga aaaagatttt ggctcaactc
     2341 attcaggcta gaatctccag ttagattgat aatattgtag cgctctgtta gttctttctt
     2401 atggtctgtc accaattggt taaagacacg agcacctgca gaaccgccaa caaacaatac
     2461 agtcggcaat ttatgattaa agtgggtttg aatatccacc aattcatctg gttctggatt
     2521 tttttgatct gaaaccttgg tcactgctcc cacatgctca accttagaca aactcgaagc
     2581 ttgctcaaag gttgaataca tcttagtcgc aaatttatag gcgattttat tggccaagcc
     2641 catagacagg tcagattcgt gaataaagac aggcactcct gacacacgcg ctgcgataac
     2701 aggcgatact gagacaaagc ccccctttga aaaaagggtc tgtggacgca gtcgcaacat
     2761 gataaagagc gattggacaa ttccccaacc aactttgaag acatccagca tattttgcca
     2821 agagaaatag cgacgcaatt ttccagtcgc aatggagtgg aaagtgacat ccaaacctga
     2881 cttaaggatt tcttggtgtt cgataccacg cttgtccccg atatagtgga cttcccaacc
     2941 atcttcgatg aacttgggca ttaacaaaag attgagggta acgtgtccaa ccgtcccccc
     3001 acctgtaaag acaatttttt tcatatttta ttcttttaac tccgctactg tgtcgataaa
     3061 gaggtcgcca cgtacttcaa agttagcata catatcccag ctggcattgg caggactaag
     3121 aagaaccaca tctccttgag tcgcaagctc ataggccttg cgggtcgcat ctgcaatatc
     3181 tgtcgcctcc acataagcga caccagcctt gtctgctgcc cgtttgacac gttctgcaga
     3241 ttgacccagg atgaccatct tcttgagtcc agtaatgtct ggcaccaatt cgtcaaactc
     3301 attgccacgg tccaaaccac ctgcaatcaa gacgaccttg ctgttgtcaa atcctgataa
     3361 ggctttttga gtagccaaga tattagttga tttactgtcg ttatagaatt taacaccctt
     3421 gatgtcatcc acaaactgga gacggtgttt gacaccaccg aaggctgaaa gagtttcctt
     3481 gatggtttga ttgtccacat cacgaagctt ggctacagca atagtcgcaa gggcattttc
     3541 cacattgtgg ctacctggaa caccgatttc attcgctgcc atgactactt caccacggaa
     3601 gtagagttga ccatcttcca gataagctcc atcaaccttt tcaagtgttg aaaatggtat
     3661 aacagtggct tctgtcttgg aagtcaagtc ttttgccaag tcttgattaa agttcaagac
     3721 aaggaaatca gctgctgtca tcttgttctg gatattccac ttggctgcta catattcctc
     3781 aaataatcca tgataatcga tatgagttgg cataaggtta gtaataaccg caatctctgg
     3841 atgaaattct tgaacaccca tgagttggaa agaagaaagt tccataacaa gcgtgtcctt
     3901 gtccgacgca gtttgggcca cttgactagc tggatagccg atattccctg ataaaagacc
     3961 atgttgccca gcagcagtca aaacttcccc aatcatagtc gttgtggttg tcttaccgtt
     4021 cgatcctgtg ataccaataa tcggtgcttc tgaaatcaaa taagccaatt ccacctcagt
     4081 caagactgga attcccttgg ccaaagcctt ttcaatcatg ggattgttgt aggggatacc
     4141 tggatttttc accataaggg caaactcttc atccaagagt tccaaaggat ggccacctgt
     4201 aatgaccttg atcccttctt ccagcaaact ttgggcagct ggattgtcct cgaatggttt
     4261 cccgtcattt actgtcacta tggcgcctag cttatccaac aaacgagctg cagattcacc
     4321 agacttggcc aaacctaaaa caagaacttt cttattttta aattgatcta ttactttcat
     4381 gtctcgaact ccatttctac tcctactatt ttaccatttt tatggaaata aaaaagccac
     4441 aaagtgtgtt tgtgactctt tcttctaact gaatcttacc atattatcta tgtgataaat
     4501 cggtaactcg aatgacctga tccacttgct cccaaatcag aggattgtgg gtcgcaataa
     4561 taatggtccg attcggattt tttaaagatt ctaggatgga aagtaattcc tcagagtttt
     4621 tggggtctaa ggaagcggtt ggttcatctg caagaatcaa aggcggatcc tttaaaatta
     4681 tcttcgctaa tgcaacacgt tgtgcttctc ctcctgataa ctcaaatata ggttgcttca
     4741 aatccaaata agagaggttt acacggttta gagcttgttt catcaaagag attttctctt
     4801 tttccttcaa ctttttacca actaaaccca gattgagatt ctctttgacg gtttggcttt
     4861 caattaagcc aaaatcttga aataagtatc ccaagtaatc tctaaagaaa acagaaggct
     4921 tgatgtcctt aagagaagtg ccatcataga tgatttgccc tttgtcatat ggctctaatc
     4981 gtccaatcat attcaagagt gttgtcttac cacagccact tgtaccgatt aaggcataaa
     5041 ttttcccacc ttcaaaatga agatttgtat ctgaaaatag ctgacggctt ccaaattttt
     5101 tagatatatt ctttagttca atcatcctat tttcctttca taattgtcat agaaacacga
     5161 gattctttct gcgcttgacg gtaaagcgtc agaactgcac tagctagaaa gactaataaa
     5221 gtgagcaagc caatcaccaa gtctcgactg cttaaaataa agagactagc accaaataca
     5281 aaactggcaa attgactaac catatactga gcatgtgttt caaaaaatcg taaacctgaa
     5341 attcgtttaa tcaaaatatc tcggcggaat tgctcgaaat atagaagatt gacagaataa
     5401 aatagtaaca aggaactagc tatccccaca atagctccta agattaaagt tgctgtttca
     5461 gtttgaactt cattataacg agttagataa acacttcttc cttctttaag ataggatact
     5521 tgctcataaa ttccagcttt cttcaagagt tctagcccac tctcatatcc tttgataaag
     5581 agttgttttc cagcattgat agaccaacta gataaggata taaaactatc acctgtagaa
     5641 gtcggcgtga atacaactaa aatcggatca atcaaatact gagtggatac gggattctca
     5701 ccgttattat aaacaaaccg cttttctccc attgaaagat aactaacgtg cgctttcatc
     5761 tcataatcca aaggagcact tgcctcctca ccagattttc cataataact caatctttct
     5821 tcaaaaactt tcttaagttc tgcttctcga gagcgcaaat gttctgggag caagaggata
     5881 aactcacctt tttggagatg ggctaacttc tgtttggtct cagcatctac cacgaccttt
     5941 tccttgtcca aataactggg actaacatag agcgtattag catctggact ataggtatcc
     6001 agtgtctctc cctgttcatt ttttccttgt ggattggcaa aatggagcag attatccttt
     6061 acataaagag cttgttcttc ttcgattgct tccttggcaa aggcatacca cttgctctga
     6121 ttttctgtat cttttcctct atcacctaag ccaaaggaaa tctggtaata gtctgctcta
     6181 tcctgccatg cttgttttga aatttcaagt tctttcaatc gttggtaaga cgtcaaacct
     6241 gtcttaacag cgtagcctac tgtaaaaaca gctactaact gacacaatag ggttaaagcc
     6301 atcaagcgtt taaggggtaa tcttccctta ataacgggaa ctaatgcttt gtaactcaaa
     6361 ctcattaggt aaaggagcat tagtaaaatt gaaatcgcca ataaaaacaa cagatagaaa
     6421 ctaatcccaa aaccataggt ggctaacaag ataggataaa acaaaccttg actaaaaaga
     6481 acgactcccc cacctaggaa ggaaaggagg gctgatagaa ggagccattt gatatcagta
     6541 gataaagaat gccccatgat ggataagaga gtctgaccag aaaagagttt tatacctgct
     6601 gctctcattt ccttaatccg agtgataatc actaaagcaa agaaagataa gccaaatatt
     6661 gctaaactaa ttaaaataag gggatttagt aatattcgaa aagcaagaaa atagggcggt
     6721 atctttcggt cagcacttgc tttataaccc aaatctccta atttatcggc aagcttttct
     6781 ttcgtcaagg agcctgacaa aaggagataa ctatttagcg gatcactacg ttcacgactt
     6841 tcttggctag cttcttggaa ttcttttggt aaagttccct gaccataagt tgcataagta
     6901 aagtgagtcg tcccatcctt actcggctct acaattcttc tagctattaa actctgttct
     6961 gagtttgcaa aattctccaa ttcctgttca aatacctcac gcgtcggttc ctgagtatct
     7021 tttttgacac gaagtaaaga aacggaatca tagcttgcat ataaatattg tggcgcacgt
     7081 aagacaataa tccaagcaag gaagaagctg agaaaaaaag ttgataataa tatgaatagt
     7141 ttcttcatag tagactcctt gtaaacaaaa ttccccctgt aatttcttac aaggggaacg
     7201 atttaaatca atgaacgatt agtcataatc acagtaaaat gctacttgtt ctccccattt
     7261 agtccaaata catgcatagg aggtttttcc agcagaagct tctcctttat ctgaatgact
     7321 attccatcta cttacaagag aagaataatg atatttttta ccatgatagt aatttgaaaa
     7381 agcccaacca cctcctgaac cttctccata tgtccatact cctccatctg gatattgtac
     7441 agcagctgat gcagctccca ataatgtaaa acttgaaata agagctagag caagtaatct
     7501 atgttttttc gttttcattt tattttttct ttcaaaaaaa tcacaccttg agcaacaatg
     7561 caacaaaata aatcctcctc tctcttttgt tgaaatcgct ttcttatgtg ataagaataa
     7621 catttttatt atttgttgtc aaggaaaaaa tcgaattttt t
//
LOCUS       DAVMEF010000090         7591 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMEF010000090 DAVMEF010000000
VERSION     DAVMEF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.090"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..227
                     /locus_tag="VYQ30_001401"
     CDS             <1..227
                     /locus_tag="VYQ30_001401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966499.1"
                     /translation="GTDCSKHRFKVVDRTDEVSSKHCFEVVDRTDEVRSKHCFEVVDR
                     TDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(549..1685)
                     /gene="dnaJ"
                     /locus_tag="VYQ30_001402"
     CDS             complement(549..1685)
                     /gene="dnaJ"
                     /locus_tag="VYQ30_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1966500.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGTNGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKVPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRGKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1855..2477)
                     /locus_tag="VYQ30_001403"
                     /pseudo
     CDS             complement(1855..2477)
                     /locus_tag="VYQ30_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756349.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            complement(2782..4605)
                     /gene="dnaK"
                     /locus_tag="VYQ30_001404"
     CDS             complement(2782..4605)
                     /gene="dnaK"
                     /locus_tag="VYQ30_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1966501.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(5033..5557)
                     /gene="grpE"
                     /locus_tag="VYQ30_001405"
     CDS             complement(5033..5557)
                     /gene="grpE"
                     /locus_tag="VYQ30_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1966502.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(5584..6618)
                     /gene="hrcA"
                     /locus_tag="VYQ30_001406"
     CDS             complement(5584..6618)
                     /gene="hrcA"
                     /locus_tag="VYQ30_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1966503.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6782..7291)
                     /locus_tag="VYQ30_001407"
     CDS             complement(6782..7291)
                     /locus_tag="VYQ30_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1966504.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         2136 a         1698 c         1448 g         2309 t
ORIGIN      
        1 taggcacaga ctgctcaaaa caccgtttta aggttgtgga tagaactgac gaagtcagct
       61 caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagatcaaaa cactgttttg
      121 aggttgtgga tagaactgac gaagtcagta accataccca tgacaagccg acgctgacgt
      181 ggtttgaaga aatttttgaa gagtatcatt caccatttca caactaaatc gaagactttc
      241 tgccgtaatg aaaacacctg aaacagcttg gtttcagctg tccggaaact ttaagactta
      301 ggttcaaagt ttaggtatgg aacttcgaag gaggtcgcta ccgtccgtca ttacttagga
      361 aagtcttaaa aaatctataa agtaaaaaga gcccgacagc tctttttgtt tatactcaat
      421 gaaaatcaaa aagcaaacta ggaaactagc cacgggctgc tcaaaacact gttttgaggt
      481 tgtagatacg actcacatat atacggcaag gcaacgctga cgtggtttga agagattttc
      541 aaagagtatt attctccatc aaaggcatct tttatatggt caaagaagcc ttttttcttt
      601 ggatttactt tcaagtcacc agcagccgcg aattctttca aggctacttt ttggcggtcg
      661 ttcaaacctg tcggtgttac gacattaaca gtaacgtatt ggtcaccaac tgcaccgcca
      721 cgaaggctcg gtgccccctt accacgtagg cggaatttct taccagtctg agttccctct
      781 ggaataacca attcaacatc accgtgaaca gttggaatat ctactgtatc accaagagcc
      841 gcttgaacaa agttgaggtt gagattgtag aagatagtcg ttccttcacg ttcaaacttg
      901 tcgctagctt ctacagaaac tactacatac aagtcaccat aaggtccacc gttaaagcct
      961 gcttcacctt gaccagcgag gcgaatttgt tgacctgttt ccacaccagc agggactttc
     1021 acatgtacgc tatgagcttg tttctcatga cctgttccat gacaggttgt acatggatat
     1081 ttgatttctt ttcctcgacc gtgacagaca tcacaggtta cttggcgacg catcatacca
     1141 agaggagtct gcgtatcgac gttaatgaca ccagcgccat gacagcgtcc acaagtgact
     1201 ggacttgtcc ctggcttagc accagagcca ttacatgtac gacagccagc ttcacgatga
     1261 tacttaactt ccttctcagt tccgaagata gcttcttcaa aggtcaaatt gacacgatac
     1321 tggagatcat ctccttggcg aggagcgttt ggattgcgcg aagaaccgcc tccaccgaag
     1381 aagcttgaga aaatgtcctc aaaaccaccg aagccacctg ccccattgaa accgccgaaa
     1441 ccaccagctc caccaaaacc accattggtg cctgcagcac catactggtc ataggcagca
     1501 cgtttttggt cgtcactcaa agtctcatag gcttcttgaa cttccttgta cttgtcctca
     1561 gcaccaggat ccttgttgat atctgggtga tattttttgg aaagcttacg ataagccttt
     1621 ttgatttcgt ctgccgaagc gtttttggat acccccagac gatcataaaa ttcagtattg
     1681 ttcataattc aagatacaaa gggcgtcaaa cggacacagc catctaggag ttttttggca
     1741 cagtccgtag cccgtgttca gttacgaaca cccttattcc tttctattga tgttctgaat
     1801 cgaaccacca tttaggtctg gttcaattcc tttctataat ttataagcaa agcattagag
     1861 gggattttct atactctgct tcacttcatc aaactctttg tccgatagag taaaaatcaa
     1921 gccctcttct gcgcatactc gttttgttct ttaaaatagt tgtaaatatt ttctgccaaa
     1981 cctagaccaa gaatcacttt tcttgcaaac tctatatgtg caaagccaat agcaggaatg
     2041 atatttttat cttgcaggac agcttttgct ttgaaatttt ctcgaggaag aaattcaaaa
     2101 tagtcaaaga agttttgcca aattcaacct gtaaatttcg tgtcgttcaa taagcctgct
     2161 ttcgccaata aaaggggcgc tgaagaaatg gctgcaatta ggatatcttg ctcactaaga
     2221 cttctcaaaa acgagatcaa tttctcatct tgtagagcat accattcctg gcaaaatcac
     2281 acaagaataa tcttctatac ggatctgatc caatgttttc gtcggttgac aggacaaacc
     2341 atcctcagag accaccatcg aataatctga agctacataa tcaatcgtga tgtcaaaaga
     2401 cagagctaaa gtacttgtta aagaggctat ctcaaaagag aaaaattagg ataaatgaca
     2461 caaagtactt ttttcataag caacaccctc tattttatcg aaaaacagag gctgggttgc
     2521 aacctctgga tttcttccta tcattacgac gttcaaaaat aaagtcgatt atactttgtt
     2581 tttgaactag taaggcgttt aggcaagtcg ccatagcgat tgccgtagaa tgacaatcac
     2641 tttagtgatt agtcattctt aacgagttag gcttaactga gtttcggcta aaaacttcgg
     2701 aaaaaagata agccatcttt tgtgcatcgc acaaaacgat ggcttcctat ttttcttttg
     2761 ttttcttaac gcctcaacat cttacttttc cgtaaactct ccgtctacga cgtcatcgcc
     2821 tgcgttccct gttgcttgtg cgccttctgc tccttcttga gcttgttgcg ctgctgcggc
     2881 ttgttcgtag agtttaacag caagtccttg agctttttcg ttcaatgctt caagttttgc
     2941 tttcatgtcg tccaagttgt tgtcttcttg agctttctta aggtcatcaa gggcagcttg
     3001 ggcagcgtca cgttctgcgt cgaagccttt accttcagtt tccttgattg tcttttcagt
     3061 cgcaaagatt gcttggtcta cttcattacg aaggtctact tcttctttac gtttcttatc
     3121 ggcttcagcg tttgcttctg catctttcat catgcggtcg atttcttcgt cagtcaaacc
     3181 tgagttcgat tggatgacaa tagtttgttc tttttgagtt ccaaggtctt tggccttaac
     3241 agacacgata ccgttcttgt cgatgtcaaa tgttacttcg atttgaggaa ttccacgagg
     3301 tgcagctggg atatcagtca attggaagcg tccaagagtc ttgttatctg ctgccattgg
     3361 gcgttcacct tgaagaacgt ggatatcaac ggctggttgg ttgtctgctg ctgttgagaa
     3421 gacttgtgat ttagatgttg ggattgtagt gttgcgatcg ataagttttg taaatactcc
     3481 acccattgtt tcgataccaa gtgacaatgg cgttacatca agaaggacaa cgtccttgac
     3541 atcaccagta atcacaccac cttggatagc cgcacccata gcaactactt catcagggtt
     3601 tactgatttg tttggttctt taccagtttc agctttaaca gcttcaacaa cggcagggat
     3661 acgagttgaa ccaccaacaa ggataacttc gtcgatttct gacaagctca aacctgcatc
     3721 tgaaagggct tgacgaactg gaacttttgt acgttcaaca aggtcacgag tcaaatcatc
     3781 aaatttcgca cgagtcaaag tcatttccaa gtgaagaggt ccagcctcac ctgcagtgat
     3841 aaatggcaag ctgatttgtg ttgaagttac accagaaagg tctttcttcg ctttttcagc
     3901 cgcatctttc aaacgttgca ttgccatctt gtcagtagac aagtcgatac cgttttcttt
     3961 cttgaattct gctaccaagt ggtcaatgat tttttggtca aagtcgtcac caccaagttt
     4021 gttgtcccct gcagttgaca atacgtcgaa gacaccgtca cccaattcaa ggatagagac
     4081 gtcgaatgta ccaccaccaa ggtcaaatac caagattttt tcttctttgt cagtcttgtc
     4141 caaaccataa gcaagagctg ctgcagttgg ttcgttaaca atacgttcta cttcaagacc
     4201 agcaatttta ccagcgtctt ttgttgcttg acgttgagcg tcgttgaagt aagctggaac
     4261 tgtgataaca gctttggtta ctttctcacc aaggtagtct tcagcgtagc ctttcaagta
     4321 ttgaaggatc atagctgaga tttcttgtgg agtgtattct tttccatttg cagaaacttt
     4381 ttcagaagtt cccatcttag atttgataga gataactgta tctgggtttg tgactgcttg
     4441 acgttttgca gcatcaccaa cgatgatttc tccgtttttg aatgagacta cagatggagt
     4501 tgtgcgattc ccttctgggt ttgcgatgat tttgctttca gttccttcaa gaactgcaac
     4561 tgctgagttt gttgtaccta agtcaatacc gataatttta gacatgtgtt tttctccttg
     4621 ttagttaatt ttctattttt ctttttgata ctcttcttaa gtggcgacgc cgtcagagct
     4681 tgacttcgtc aatctctttg actaaacttt gagcctaagg tctcaaaatt tgcgcaaata
     4741 gcaccacggc gaagagtatc gtctttggtt cgcttcgctc actattacaa agccaaacat
     4801 attttatctt tcttcatagt ttattgtcgt ttcggacaag ttttctattc tgtaaattgc
     4861 gacacgagga gtcgaaatcg attttatttc gacgacgagt tagtaaggaa gctaggcaaa
     4921 cggcaatcga acacgcccta agctctgagt aaaaaagata gattttcttg tgttcatcga
     4981 acacttcgtc aatctcctat ttttactgcg agctttttac gggctttgta tcttagttat
     5041 acaccactac cattgctggg cgtaggatgc ggtcatggag tttgtagcct ttttgaaaga
     5101 cttgagcgat ggtatctact gggtgttcat cgtctgctgg gagagtttgg atggccatat
     5161 ggtagttatg gtcaaattcg ccatctgctg cgatttcttc aattccttct tctttcaaag
     5221 cgtgaatcaa gctttcttgc accatcccca agcccttctt cacatcatct gtcaaacctt
     5281 caactgcaag tgcacgctca aggttgtcaa gagatggtaa gattgctttt gccaagtcct
     5341 ggctacgata acgttgcaag ttttgacgtt cttcattggc acggcgttgg atattttgca
     5401 tttctgcatg agcgcgaaga tatttgtttt cgaactcatc tgcacgttca tttgccaagt
     5461 ccaactcaga cttttcagga gttgtttctt cagctgtttc cacaacttcc tcttcttgaa
     5521 cttcttctac ttcttcattt tttatatctt gggccatgtt cgcctccttt aatgatttca
     5581 atcttaatgt acttcgtaat gattactgct gaggtagcgg taaaaatctg tcaacttcat
     5641 ggtcaaaaca cggttgacca cattgacttg attgattagc tgttggtaat ccagattaac
     5701 tggaccgata atggctagaa ttccaactcc ccgataagga atgaggaact tactactgat
     5761 taccgctagg tcagctaaac aggactcttg accgtctgca acacgaacat tttgcatctg
     5821 atcctcacgc aacccctcac gaatctccaa ggccaccttt tgcggttggt caaagaactg
     5881 ataggctgct agattggcaa aattcaagag atggaccttg cccgccatca caatgttttc
     5941 gttgaacatt tccttaaaga tgtgttcaaa gagatcgatg acattatccg ttgttgtaaa
     6001 gtaacgctgg ataatctgcg gaatctccgt ccgaatcttg tagtgaatat ctaaaacggt
     6061 gtgaccgagg aaacgttcct gaatgatgct cttcagtttc agcaaatcct cctgcaagaa
     6121 gttccttgga atcagaaact gactagtaac cgttcgcgac tcgtctaggg taaataccgc
     6181 caaggctgta tgttgcccca aaacaacgat atcaaaggct gtcaaacgtt gcctgctcgg
     6241 ctcaacatcc agtgccacta ccgtacagcc actcaggtct gttagtaagt tagcagcctc
     6301 ttgcagaata tcctccaatt tgaagaattc ctgatcaaag gctttgacaa tctcatatac
     6361 ctcattttca gccagccggt gaaaatccag tgagtgtttc acatagtact gaaaaccagc
     6421 aacacttggc atccgaccgc ttgaagtatg agccttctca agcaaccctt gtttttctag
     6481 ttccgccatg tcattacgaa tggttgcact gctagagtta atagactctt gcaaggcttt
     6541 tgatccgaca ggttcgtgcg ttttggtaaa gatgtcaata atcagattta aaatatcctg
     6601 ctgacgctct gtaaccatct catcactcct ctctgtctga tcaattagca ctcaatgcat
     6661 ccaagtgcta acttatgatt ctattataca cccttagaag agaatgtcaa gacaaaaact
     6721 caaaaaatta gcactctttt atcaagagtg ctaaaaatca atctgaagac ctagaacctt
     6781 gctattcatc tactcggtat ttccttttta gtctgaaata accatgaatt agataagaaa
     6841 tcagaaaagc atataaaaca aaaataagaa agaaagacat agaaagatcc tctctaaaca
     6901 aactcgtaca aacaacaaga ccacctaaga aagtagttgt acaggaacga agtctagatc
     6961 gcataacttc ccatttgagc cccttactca tatagacttg atcctctgga agtaaatcag
     7021 aagacgattt acgaagaatc gaacaagaac ctgctcctat caattcccaa cctctatctc
     7081 taaaatcttg cagttcacgc ttatgctctt taagaaatct agaatcatag atacgataga
     7141 taacatcgtc cggttgacct tgctcaaaat agaacaaacc aaaacgacta gttctatacc
     7201 tccaaccttt caaatgcatc tcatgtaaat attcttcttt cttgtccaaa tcaacaatgg
     7261 tgaaaatccg aaattctact ctgctattca ttgtcttacc ccaaaattag aaaacatgcc
     7321 tagcgttatt tattagataa ttctttccac ttttgactca atctccaaaa aatataagaa
     7381 atctgaatcg caaaaactat caataaaacc caatctatta tgaaaatcaa aaacacttcc
     7441 caactgaaag aactacctcc agtgacaaac tttgagaaaa acggtagtag agctaaaaag
     7501 agaagcaaaa taggaagcat ccgcattgtt aaaatccttt tggcataaaa aaatctttat
     7561 ttaaacgaaa atattatggc aaaatttacg c
//
LOCUS       DAVMEF010000091         7511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMEF010000091 DAVMEF010000000
VERSION     DAVMEF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.091"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(314..1492)
                     /locus_tag="VYQ30_001408"
     CDS             complement(314..1492)
                     /locus_tag="VYQ30_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1966505.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            complement(1658..2278)
                     /locus_tag="VYQ30_001409"
     CDS             complement(1658..2278)
                     /locus_tag="VYQ30_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1966506.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2282..3562)
                     /locus_tag="VYQ30_001410"
     CDS             complement(2282..3562)
                     /locus_tag="VYQ30_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1966507.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGIPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            complement(3623..5107)
                     /locus_tag="VYQ30_001411"
     CDS             complement(3623..5107)
                     /locus_tag="VYQ30_001411"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1966508.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVCHKTTVAAADMQVAVEAYLGL"
     gene            complement(5183..6460)
                     /locus_tag="VYQ30_001412"
     CDS             complement(5183..6460)
                     /locus_tag="VYQ30_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1966509.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6567..6650)
                     /locus_tag="VYQ30_001413"
     tRNA            complement(6567..6650)
                     /locus_tag="VYQ30_001413"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6614..6616),aa:Leu,seq:caa)
     gene            complement(6660..6731)
                     /locus_tag="VYQ30_001414"
     tRNA            complement(6660..6731)
                     /locus_tag="VYQ30_001414"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6697..6699),aa:Gln,seq:ttg)
     gene            complement(6742..6814)
                     /locus_tag="VYQ30_001415"
     tRNA            complement(6742..6814)
                     /locus_tag="VYQ30_001415"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6779..6781),aa:His,seq:gtg)
     gene            complement(6829..6899)
                     /locus_tag="VYQ30_001416"
     tRNA            complement(6829..6899)
                     /locus_tag="VYQ30_001416"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6865..6867),aa:Trp,seq:cca)
     gene            complement(6904..6984)
                     /locus_tag="VYQ30_001417"
     tRNA            complement(6904..6984)
                     /locus_tag="VYQ30_001417"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6948..6950),aa:Tyr,seq:gta)
     gene            complement(6997..7069)
                     /locus_tag="VYQ30_001418"
     tRNA            complement(6997..7069)
                     /locus_tag="VYQ30_001418"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7034..7036),aa:Phe,seq:gaa)
     gene            complement(7073..7146)
                     /locus_tag="VYQ30_001419"
     tRNA            complement(7073..7146)
                     /locus_tag="VYQ30_001419"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7110..7112),aa:Met,seq:cat)
     gene            complement(7157..7246)
                     /locus_tag="VYQ30_001420"
     tRNA            complement(7157..7246)
                     /locus_tag="VYQ30_001420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7208..7210),aa:Ser,seq:tga)
     gene            complement(7329..7402)
                     /locus_tag="VYQ30_001421"
     tRNA            complement(7329..7402)
                     /locus_tag="VYQ30_001421"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7366..7368),aa:Ile,seq:gat)
     gene            complement(7437..7507)
                     /locus_tag="VYQ30_001422"
     tRNA            complement(7437..7507)
                     /locus_tag="VYQ30_001422"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7473..7475),aa:Gly,seq:tcc)
BASE COUNT         2258 a         1794 c         1359 g         2100 t
ORIGIN      
        1 ctaacaatgt tttagaagca gttgtgtact attctagttt ccatctacta taaaatagat
       61 tgcaagaatg ttgcttaact cattacaaga tgataacttt ccattttcca ttcaaattga
      121 tactcaatga aaatcaaaaa gcaaactagg aagctagtcg caggttgctc aaagcacagc
      181 tttgaggttt cagataaaac tgacgtggtt tgaagagatt ttcgaagagt atgacaaaga
      241 aaaaacgaac gagaatcgct ttccatcacg aaagctagat ctcattcgtc aaaatgatgt
      301 tatcaagtcg ggtttatcca ttcattgaaa cgtgaacgtg atcatagtga ttttctgtca
      361 cactaccacg gtctggcatt gggttccaag tgttagctgg cccatatttg ctatcgaatg
      421 gagcatagaa acgttgtttc cagatgatgt aactaatgcc acggctggcc atattttgaa
      481 tagcatattc cgcaatctta tcccctaatt ctgaactttc tggtaccata aagtcgatag
      541 ccaaaccttt tccgtgatct ccactgtctc ctggacgata accactaaag gatgtaatgc
      601 caaacaagtt agcaatttct tctttaaagg cagctgtttg tggttgaaga cctgcatttt
      661 cagatttcgc tactgccagt ccagcataat cgggcgcagc tggagcctcg taagttgttg
      721 aaattgtttt cgtctcttct ggttgataag tagaaactgc tgctgtagct ccatttgatg
      781 atgctacttc ttttgcttct gaacttgttg cagttgtttc ggttgctttt tcttctactg
      841 gcattgtagt tgcttccact gctgactcgg tttgagagct cacttgtgtt tcctgcttct
      901 cagctggagt cgtttcctga ggagcttctt ctgcaactgc actgcttgtt tcggccgttt
      961 gctcctctgg gacagaagtg cccgtagatg gtgccacttc ttcagaagca atcactgtct
     1021 ttgtaacttc tgaacttgat gctacttctt ttggagcttc tgcaatcggt tgagaaaggt
     1081 ctgcaacctg aacagtttga tcatcaacgg tcacttgatt agtggtcaaa tctgctgtcg
     1141 cagttgtcac ttcttcacta gagtctgctt gaggtgtttg gatttcaact tctgttactt
     1201 cttctgcttc attgacagtc gttgtcaaaa cagtttctgg gaaaatcaag tccatattag
     1261 tgattttgtt cagattcgca agcactgtga catctacacc caaggcttct gcaatggtgc
     1321 tcaaagtatc accatactgt acggtataac ttgttttgtt gtccgtttta gtcaaatcgt
     1381 tttggatttg ctcaacacta cgtgcagtcc aaagaacttc ttctgcttga gttgccaata
     1441 ctggggcaaa tgacaaggct actgttgacg ctaataacat tcttttcttc attctttcaa
     1501 attcctttca aatgagtacc tgtctatcat aacacaaaaa atgtagaaaa aaagccctct
     1561 cgggcctatt taacagaact gtctattcct tgtaacaaac taggttactt gaaatagttt
     1621 gttaggtctc tgtcacaaaa ctgacacaat ctttttatca cttagtctca aaaatacggg
     1681 aaatatctgc taacgcttga atcctgtgat tcccttcata agtagactct aaaaagttga
     1741 tactttgaat cccactattc tgggcaaatt ccacatccag agtccgatcc cctatataat
     1801 aagtattatc agaattcaac tgatacttat ctagcagata ggtagccgct tctggacttg
     1861 gcttccgcac aaagccactc tgactggtta aaatctctgt aaaataggat tccaccccca
     1921 agtccttgag aatggtaaaa gcgttgttcc ccttatgagt atatataaac tgctgaattc
     1981 ctgattcgtc tgcccaagct agcacctcac gcgcacctgg catcaaaact acctgagcat
     2041 tcttctcagc cagactctgg gcacgcacct gatttagcac ctcaacatcc agatttctat
     2101 cttctgccac ccgcacaagc aaattttgca ccgaatactt gaagataaac tctctcacct
     2161 tctccttatc ataaggaata gaaaactgag caaaagtctc ctcaatccct gataaaatcg
     2221 cttcgtaaga gtccaataaa gtcccgtcta aatcccaaat aaaagctgtt ttttgcattt
     2281 cctatccttt caagatcata tagcgctggt aacgatagcg tagaaataac cagcgaaaac
     2341 cattatccaa gagagaccct gcccaaatac caggtaagcc ccaaccaagc acaatcccca
     2401 tcagatatcc tgttccaatg cggatacacc acatgcctat acttgtcgca taaaaaggga
     2461 ggcgtgcatt tcctaatccc tgccagactg ccgtatagat gactgttcct gtcgtcatag
     2521 gggtcccaag tagtgaaaac aatgtcacta gaacactagc ctccaccgct agagaatcag
     2581 tcgtatagag atgagttaat ggtataccca agacatatat actaaaggac aggggcaaca
     2641 tgaggaacag agaaagccaa aaggtttgtt tactcaaact agcaactctt ttccaatcat
     2701 cctctccaac tgctcgggcc aacagcatga ccgttgccgt agcgacgcca aaggcaggca
     2761 tatagttaaa ctgggtcaag acttctccga ttgcattccc agcaactgcc tccgtcccaa
     2821 aagaaacgac caaggcaatg atcactacat ctccagccct catcataagt cgctctccag
     2881 ctgctggtaa agccaaggtc aacagttcct tatctaaacc aaaagtgggc ttcccataag
     2941 gcagttttaa ttgtgaccac aaaatcacaa gaccaaccaa acgagacaga attgtccccc
     3001 aagcaacacc agctatcccc atatccagaa caaaaatagc tagacttgaa aaaagaatat
     3061 tcaaggcatt ggataaaaaa ctaacataga gaggcagacg tggattatgc gttgcacgaa
     3121 tcaaggctcc cagactagtc attaaaccta agagaacaat cgatccgcct accaaagata
     3181 gatacagtcc accactctca gctacatccc tctccgtccc caaaagtcct atcatctctt
     3241 tcccagcgaa gatggacaaa aatcctaaaa ggaaacttaa tagtaaggta atcttcaacg
     3301 cctcagtcac atgataggcc aacttcgact ggtctttctg ccctatgctt tttgaaataa
     3361 cactggaaat agcagctccc agagcgatga aaatcgcctg ataaatggtg ataatattac
     3421 cagctaccga aacccctgaa atagctatca atcctaaatg agcaaccaaa taactgtcca
     3481 ccattcccat tagcatctgc aaaaagtttt cacccatagc tggcaatgca atattaagaa
     3541 tgtctttatt tttcttaaac aatctctcct cctgatgaaa agaaactcag ttggtttccc
     3601 aaccgagttt actccctcta tcttaaagtc ctaaataagc ctcaaccgct acttgcatgt
     3661 cagcagctgc cactgttgtc ttgtgacaaa caggagctgt ctcaagccca tcaactgctg
     3721 gtggcactgc aacgcctgag atttcatgta attgagccaa agcttcaaag tctgttaaac
     3781 ctgcttttcc agttacagct tctactgcaa ctactgggaa cttgtaggga ctagctgttg
     3841 aagcaatcac tgtcttagtc gcatcatcag taaccgcttg gtattttcta taaactgctg
     3901 aggcaaccgc cgtatgtgga tcctcaatat aagaatctaa ctcataaaca cgcttgattt
     3961 ctgccgctgt ttcttcctca gtcgcatatt cagctgcaaa gagctccaga atctctgcat
     4021 caaaatcagt cagttcatat tgtccttgtg tattcaaggc attcatgagt tcagccgtct
     4081 taaccgcatc attccccaaa agatggaaaa tcaaacgctc caagtttgaa gataccaaga
     4141 tatccataga tgggctggtt gttaccttaa actcacgttt cttgtcgtaa acacgtgtct
     4201 tgaagaagtc tgtcaaaaca ttgttgtcat ttgaagcaca gatcaattta ccaactggga
     4261 gaccgatttg tttggcataa aaggcagcca agatatttcc aaagtttcct gttggtactg
     4321 tgaagttaat cttatcacca gccacgatct caccagtctt gaccaactga gcataggcat
     4381 aaacataata aacaatctgt ggcaccaaac gaccgatatt catagagtta gcagatgaaa
     4441 attgcaactt gttggccgct aatctttcac gaagagccac gtcgttaaac atgtgcttca
     4501 cgttggtttg cgcatcgtca aagttaccat ctatagcgat aacatgagta ttgtcaccat
     4561 tctgagtggt catttgcaac tcttgtacct tgctgacacc accctttgga taaaagacga
     4621 taatctcagt accaggcaca tccgcaaacc ccgccatagc agctttcccc gtgtcaccag
     4681 atgtcgctgt caagataaca atcttgttct ccaaaccatg tttcttagca gcagtcgtca
     4741 taaagtatgg caaaatagac aaggccatat ccttaaaggc aatcgttgaa ccatggaaaa
     4801 gttccaaatt gtattgccca tctaatttca ctaatggtgc aatagctgga gtatcaaatt
     4861 tgctatcgta ggcattgttg atacagtagt ccaactcctc agctgtaaag tcatctaaaa
     4921 atgctgacaa aactagctta gcaacttcct ggtaagaagc atctttcaat ttgtcaaagt
     4981 ccaaatctac ctttggataa gtaaccggtg taaacaaacc gccgtccgtc gccaaacctt
     5041 gcaaaattgc ttggctggca gttactgtat tgttggcatc acgcgttgat tgataaacta
     5101 atgtcattac tctctatcct ttatctatag tctcgtccat tatatcatga tttttcagaa
     5161 aattctccct ttataagaga aattataggc tcaattcttc tctcaaaggc tgtaaatagg
     5221 tcatttcttc cttatttctc accactagtc cctcctcagc tagtaggagt aagtctttgg
     5281 aaaattcaat aatcgtagtt tcttcctcat ctgtaagatt tttcttagaa aattgtctcc
     5341 ttaaagactt gtaatcataa ccaaatactt taaagaaagg tgctgtttct aagtaagctt
     5401 ctaacttgtc taaattaacc aataatccca agtgaaaagc tgcagaagca aaagtcctat
     5461 caagtggctg tgtacacaca ctacgaaact caactgttcc tcgagtcgtt aagtcttggt
     5521 actggtaact acgatgagtt tcaaaatcct tctcttgggg gtaaataata acctcatccc
     5581 cattcagagc aaatgcttgg atttcaggcg tagccaaata gtccccagcc tgaataggat
     5641 aaaaataata ggtctgccca tcacgttccg cagtaaaaat cgcagaatga tttagatagt
     5701 caaaaaaatc agcttcatca ttaaggagtc tagcattgac cccaacattc tctggataga
     5761 taccatgcat agattcttcc cagaaaatat cccttgaaat tttcgtatcc caatccgcac
     5821 ccgaaaattc agagtttgca aataaataag ccttagccgc ttcaatttga gtaaaagcat
     5881 taatcacccg taagtagttg gtttttgaaa tatccagctg aacctggctc ccacagataa
     5941 aagtaccata ttcagggaaa tgatgtaaat ctgatttaat aatatttcta ctcaaattca
     6001 aataatccat caacatctga tagcgtggat aagccactgg acaattctca tttttatccc
     6061 agttgggatg gataccacag ccaacaatag catgatttga ttcagctaac tttctctgaa
     6121 ttacattcat ataattattg aaacgatttt cgacctcttg aatcgtttca gccttaccaa
     6181 atgcaaactc aatcgttgta taggaaactt caaataaaat agcatcctga cttatcggat
     6241 ctactaactg gatcagattg ccaaaatcat ctacctttgc tacggtgaga tccaaagtag
     6301 aaactaaata atgaaataga tcctttataa cttcaacatc tgtagcatcc ccttctaaac
     6361 ttgcaacagg atactccaac tcaattccga caaacaattc aggattttct ttaatatttt
     6421 tcaaataccg atgttttaat aaatcgatag aacaagacat actcccctac actttcttaa
     6481 tctaaataaa atttgaataa ctataattat accaaaaata atataaaaaa ggaacgaaga
     6541 ataacaacgt tcctaacact atactatacc ggcggccggg gtcgaaccgg cacgtccttg
     6601 cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatagtaac
     6661 tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg
     6721 gctatacccc aatataaaat aggcgagtga tggggatcga acccacgcat gccagagcca
     6781 caatctggtg tgttaaccac ttcaccacac ccgccataat tctattaaca cgggcagtag
     6841 gaattgaacc cacactgaag gttttggaga ccttagttct acctttaaac tatgcccgta
     6901 aaatggaagg ggagggattc gaacccccga acccgaagga gcggatttac agtccgccgc
     6961 gtttagcctc ttcgctaccc ttccaaaata tataaatggc gcgagacgga atcgaaccgc
     7021 cgacacatgg agcttcaatc cattgctcta ccaactgagc taccgagcct tattgcggga
     7081 gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccga gctgctccat
     7141 cccgcgttaa tataaaagga ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg
     7201 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccaaaa tagtttatac
     7261 gggaacaagc ccgtgtcctc ttagtgaaca gataatattc caatccccta gtaatggact
     7321 aaacactatg ggcacgagtg gactcgaacc accgacctca cgcttatcag gcgtgcgctc
     7381 taaccacctg agctacgcgc ccaagtcaaa aacttggtac aaagaacaaa gttcaaagcg
     7441 ggtgacgaga atcgaactcg cgacaacagc ttggaaggct gtagttttac cactaaacta
     7501 cacccgctaa a
//
LOCUS       DAVMEF010000092         7328 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMEF010000092 DAVMEF010000000
VERSION     DAVMEF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.092"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..154
                     /locus_tag="VYQ30_001423"
     CDS             <1..154
                     /locus_tag="VYQ30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1966510.1"
                     /translation="IEYIKARNIKTSSDIELESDVDGDKSDNLPVDIKILGQHIEVYS
                     VPKKQS"
     gene            354..3629
                     /gene="rexB"
                     /locus_tag="VYQ30_001424"
     CDS             354..3629
                     /gene="rexB"
                     /locus_tag="VYQ30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1966511.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTILDDIGLGLAFYKCLAELNPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3626..7276
                     /gene="addA"
                     /locus_tag="VYQ30_001425"
     CDS             3626..7276
                     /gene="addA"
                     /locus_tag="VYQ30_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1966512.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLVRVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSFQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2218 a         1558 c         1573 g         1979 t
ORIGIN      
        1 tattgaatat ataaaggcac gtaatattaa aacttcttct gatatcgaat tagagtccga
       61 tgttgatggc gataaatcag acaatctacc agtcgacatt aagatactgg gacaacacat
      121 tgaagtgtat tccgtaccta agaaacaatc ataaaactca aagccttgtt accgaggctt
      181 ttttattatg tgattttcag tagccagact tattctatct aatacaagta aagctaagta
      241 tttaagattt agcagatgat gaagaaaaga aaggatggcc taaatatttt aacagattta
      301 cgaggctaat catggtataa tatagggtga tgaaaaattc caacgaggct gagatgaaat
      361 tactttatac tgatattcgg acttctttga cagaaattct aacaagagag gcagaagagc
      421 tagttgcagc tggcaagcgg gtcttctaca ttgcccccaa ctctctttct tttgaaaagg
      481 aacgcgccgt gctggaatgc ttgtcccagc aggcttcttt ttcgattacc gtcacgcgct
      541 ttgctcaaat ggctcgttat ctggtcttga atgatttacc agctaaaact attcttgatg
      601 atatcggtct tgggttggcc ttttacaaat gccttgccga actcaatccc aaggacttgc
      661 gtgtttatgg cgctattaag caggatcctc aattgatcca gcagttaatt gagctttacc
      721 atgagatgac caaatcccag atgaattttt tggacttgga gaatttaaca gatgaggata
      781 agagggcgga tttactcttg atttttgaga aagtaacagc ctatcttaat caaggtcagt
      841 tagcccaggg aagtcagttg tcccatttga ttgaggctat tgagaatgac aaggtaagta
      901 gtgattttaa tcaaatcgcc ttggtcattg acggctttac tcgtttttct gctgaggaag
      961 agcgggttgt ggacttactt cacggcaaag gtgttgagat tgttatcggg gcttatgcta
     1021 gtaagaaagc ctataccagt ccttttagcg agggcaatct ctaccaagcc agcgtaaaat
     1081 ttctccatca tctggcttct aaataccaaa cgcctgctca ggactgttct caaactcatg
     1141 agaagatgga tagttttgac aaggcctctc gtttgttgga gtcttcttat gacttttcag
     1201 aactcgcttt ggatgtcgat gagaaagacc gtgaaaattt acaaatctgg tcttgtttga
     1261 cgcaaaagga ggagttggag ctagtagccc gtagtattcg tcagaaatta catgagaact
     1321 cagacctgag ctacaagcat tttcgtattc tcttggggga tgtagcttct taccagttat
     1381 ctctcaaaac catttttgac cagtatcaga ttccttttta tcttggtaga agcgaagcca
     1441 tggctcatca tcccttgact cagtttgtcg agtctatttt agctttaaaa cgttaccgtt
     1501 ttcgtcagga ggatttgatc aatcttctta gaactgactt gtatactgac ctcagtcagt
     1561 ctgatattga tgcttttgag caatatatcc gctatcttgg tatcaatggc ttgccagcct
     1621 ttcagcaaac cttcaccaaa tcccaccatg gaaaatttaa tcttgagcgt ttgaatgtcc
     1681 tccgcctgag aattttagca cctcttgaaa ccctctttgc cagccgaaaa caaaaggctg
     1741 aaaacctcct acaaaaatgg agtgtctttc taaaagaagg agctgtgacc aagcagttac
     1801 aagatttgac aaccactttg gaagctgtag aacaggaaag acaagccgaa gtttggaagg
     1861 ctttctgcca tgttttagaa caatttgcga ctgtttttgc tggttcacag gttagtctgg
     1921 aagacttcct agccttgctc cattctggaa tgagtttgtc ccaataccgt accattccag
     1981 caacagtgga cactgttctg gtgcagagtt acgatttgat tgcaccattg actgctgact
     2041 ttgtctatgc tattggacta actcaggaca atttaccaaa aatttctcaa aacaccagtc
     2101 ttctgacaga tgaagaaagg caaaacctaa accaagcgac cgaagaaggc gttcaattac
     2161 tgattgccag cagtgaaaat ctcaagaaaa atcgctacac tatgctttcc ttggtcaatt
     2221 ctgctcgtaa gcagttgttc ttgtcggctc caagcctttt taacgaaagt gaaagtaagg
     2281 aatctgccta tcttcaagag ttgatccatt ttggatttag gcggagagag aagaggatga
     2341 atcacaaagg actgtctaag gaggatatgg ggtcctatca cagtcttttg tctagtctgg
     2401 ttgcctatca ccagcagggt gagatgagcg atactgagca agatttgact tttgtcaagg
     2461 ttctgtcgcg tgtcataggt aaaaaactag atcagcaagg tctggaaaat ccagctatcc
     2521 caaccagtcc aagcagcaag accttagcca aggacacctt gcaagctctc tatcctgcca
     2581 aacaggagtt ttacctgtct acgtcgggtt tgacagagtt ttatcgcaat gaatacagtt
     2641 atttcctacg ctacgtttta ggcttgcagg aggaattacg tttgcatcct gatgcccgta
     2701 gtcacgggaa tttcttgcat cgtatctttg aacgcgcctt acagttgcct aatgaagatt
     2761 cctttgacca acgtctagaa caagctattc aagaaaccag tcaagaacgc gaatttgaag
     2821 ctatttatca agaaagtttg gaagcccagt ttaccaagga agttttgctt gatgttgcac
     2881 ggacgactgg acatattctc cgacacaatc cagccatcga aaccatcaaa gaagaagcaa
     2941 attttggtgg aaaagaccaa gcctttattc aattagacaa tggacgcagt gtctttgtac
     3001 gaggcaaggt ggaccggatt gaccgtttga aagctaatgg agcgatagga gtagtagact
     3061 acaaatccag tctgactcag ttccagtttc ctcatttctt taatgggctc aattctcagt
     3121 taccaaccta tcttgctgcc ctaaaaagag aaggggagca gaactttttc ggcgccatgt
     3181 acttggaaat ggctgaacct gtccaatctc tgatggcggt aaaaagtctg gcgggtgcag
     3241 tagtagaagc cagcaagtca atgaaatacc aagggctctt cttggaaaaa gaaagcagtt
     3301 atttaggcga attttataac aaaaacaagg ctaatcaact gacagatgag gaatttcagc
     3361 tcctactgga ctacaatgcc tatctttaca agaaagctgc tgagaagatt ttagcaggcc
     3421 ggttcgccat caatccttat actgaaaatg gcagaagcat tgccccatac gtccagcaac
     3481 atcaggctat tacaggcttt gaagccaatt accatctggg ccaagcccgt ttcctagaaa
     3541 agttggacct agctgatggc aagcgtctgg tcggagaaaa actcaagcaa gcttggcttg
     3601 aaaaaataag agaggagttg aatcgatgaa gcttattccc tttttaagtg aggaggagat
     3661 tcaaaaactg caagaagcag aagcaaattc gagcaaggaa cagaagaaaa ctgccgagca
     3721 aatcgaagct atctacactt ctggccagaa tatcctggtc tcagcatcgg ctggttctgg
     3781 aaagaccttt gtcatggcag agcgcattct ggaccaattg gcgcgtggtg tcgaaatttc
     3841 tcaactcttt atctcaacct ttacggtcaa ggctgcaact gaacttaaag aacgtttaga
     3901 gaaaaaaatc agcaagaaaa tccaagaaac agatgatgtc gacctcaaac aacacttggg
     3961 acgccagttg gcagacctac ccaacgctgc catcggaacc atggactctt tcacacaaaa
     4021 attccttggc aaacatggtt atctgcttga tattgcacct aatttccgta ttttacaaaa
     4081 tcaaagcgag caacttcttc tcgaaaacga agtctttcat gaggtctttg aagcccatta
     4141 ccaaggtaaa cagaaagaga cctttagtca tttgctgaaa aactttgctg ggcgtggcaa
     4201 ggacgaacgg ggtctgcgcc agcaagtcta taaaatttat gacttcctcc aatccaccag
     4261 taatcctcaa aagtggctga gtgaatcttt cctcaaaggt tttgagaaag ctgattttac
     4321 cagtgaaaaa gaaaaactga ctgagcaaat caaacaagcc ctttgggatt tggaaagctt
     4381 tttccgttac catctggata acgatgccaa ggagtttgca aaggctgcct atttagaaaa
     4441 tgttcagtta attctggatg aaattggctc cctaaatcag gagttcgata gtcaggctta
     4501 tcaggcagta cttgtgcgtg ttgtcgccat ctctaaggag aaaaacggtc gtgctctgac
     4561 taatgccagc cgtaaggctg atttgaagcc cctggctgat gcctacaacg aagagagaaa
     4621 gacccagttt actaaactag gacaattatc agaccagata gcgattctcg actatcaaga
     4681 acgttatcat ggagacactt ggaaactagc taaaaccttc caatctttca tgagcgattt
     4741 tgtagaggct tatcgtcaga gaaaacgaca ggaaaatgcc tttgaattcg ctgatatcag
     4801 ccattacacc attgagattt tagaaaattt cccacaagtt cgtgagtctt atcaggagcg
     4861 cttccatgaa gtcatggtcg atgagtatca ggataccaac catattcaag aacggatgct
     4921 ggaattgttg tctaatggcc acaatcgctt tatggtggga gatatcaagc aatccatcta
     4981 tcgtttccgt caggcagacc cgcagatttt caatgagaaa ttccaacgct atgcgcaaaa
     5041 tcctcaagaa ggcaagctca ttctcctcaa ggaaaatttc cgtagtagtt cagaagtgct
     5101 gtcagcaacc aatgatgtct ttgaacgtct catggaccaa gaggtcggcg aaatcaacta
     5161 tgataacatg caccagcttg tttttgccaa taccaaactg actcccaatc cagacaacaa
     5221 ggctgaattt ctcctctacg acaaggacga tacaggtgag gaagaagaga gtcaaacaga
     5281 aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag attctgaaac ttcatcaaga
     5341 aaaaggtgtt gcctttaagg aaattgccct tctgacctcc agccgcagtc gtaatgacca
     5401 gattctcctc gccctgtctg agtacgggat tcctgtcaaa actgacggag agcaaaacaa
     5461 ttatctccaa tccctagaag tgcaagtcat gctagacact cttcgtgtca ttcacaatcc
     5521 cctgcaagac tacgccttgg ttgcccttat gaagtctcca atgtttggtt ttgatgagga
     5581 tgagctagca cgtttgtccc ttcagaaagc agaggataaa gtccacgaaa atctctatga
     5641 gaaactggtc aatgcacaaa aaatggcaag tagtcaaaaa ggcttgattc acacagctct
     5701 agctgaaaaa ctaaagcaat tcatggatat cctagcttct tggcgcttgt atgccaaacc
     5761 ccactctctc tatgacttga tttggaagat ttacaacgac cgtttttatt atgactatgt
     5821 tggggctttg ccgaatggtc ctgctaggca ggccaatctc tatgccctag cactgcgtgc
     5881 tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt tttattcgta tgattgacca
     5941 agtcttagaa gcccagcacg atttggcaag cgtggccgtc gcaccgccaa aagatgcagt
     6001 agagctcatg accatccaca agagtaaagg gctggagttt ccttacgtct ttatcctcaa
     6061 tatggatcaa gatttcaaca agcaagactc tatgtcagaa gtcattctca gtcgtcagaa
     6121 tggtcttggt gtcaaatata ttgccaagat ggagacaggg gcagtagaag accactatcc
     6181 taaaaccatc aaactctcca ttcctagtct gacctatagg cagaacgaag aggaattaca
     6241 gctagcaagc tattctgagc agatgcgttt gctgtatgtt gctatgacgc gggctgagaa
     6301 aaagctctat cttgtcggca agggttctcg tgaaaagctg gaatccaagg aatacccagc
     6361 agccaaaaat gggaaactaa atagcaatac tagactgcaa gcacggaatt tccaagattg
     6421 gctttgggct atcagtaaag tgtttactaa ggacaagctc aactttagtt atcgttttat
     6481 tggcgaagat cagttgacca gagaagctat cggagagttg gaaaccaaga gtcctctcca
     6541 agatagcttc caagcagaca atcgtcagtc agataccatc aaagaagctc tggaaatgct
     6601 gaaggaggtg gaagtttata atactcttca ccgcgcagct attgaacttc ctagtgttca
     6661 aaccccaagt caaatcaaga aattttacga accagttatg gatatggaag gtgtcgagat
     6721 tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc gatttgccag atttttcaac
     6781 caaagaaaag gtaactggag ctgagattgg tagtgctact cacgaactca tgcagagaat
     6841 tgacctcagc cagcaactaa cccttgctag cctaacagaa acactcaaac aagttcaaac
     6901 tagccaagct gtcagagaca agatcaatct tgataaaatt cttgctttct ttgacacagc
     6961 actcggtcag gaaattcttg ctaataccga ccatctctat cgcgagcaac ctttctccat
     7021 gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc cgtggtatcc ttgatggcta
     7081 tctgctttac gaaaacaaaa ttgttctgtt cgactacaag acagaccgct atgatgaacc
     7141 aagtcaactc gtagaccgct atcgtggtca gttagctcta tacgaagagg ctttatcacg
     7201 agcctattcg attgaaaata ttgaaaaata cttgatttta ctcggtaaag acgaggttca
     7261 agttgtaaaa gtataactta tactcttcga aaatctcttc aaaccacgtc agcttcgcct
     7321 tgctgtat
//
LOCUS       DAVMEF010000093         7305 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMEF010000093 DAVMEF010000000
VERSION     DAVMEF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.093"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(370..1218)
                     /gene="dprA"
                     /locus_tag="VYQ30_001426"
     CDS             complement(370..1218)
                     /gene="dprA"
                     /locus_tag="VYQ30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1966513.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1314..2003)
                     /locus_tag="VYQ30_001427"
     CDS             complement(1314..2003)
                     /locus_tag="VYQ30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1966514.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2015..2893)
                     /locus_tag="VYQ30_001428"
     CDS             complement(2015..2893)
                     /locus_tag="VYQ30_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1966515.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2883..3752)
                     /gene="licA"
                     /locus_tag="VYQ30_001429"
     CDS             complement(2883..3752)
                     /gene="licA"
                     /locus_tag="VYQ30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1966516.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3769..4791)
                     /locus_tag="VYQ30_001430"
     CDS             complement(3769..4791)
                     /locus_tag="VYQ30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1966517.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4796..5503)
                     /locus_tag="VYQ30_001431"
     CDS             complement(4796..5503)
                     /locus_tag="VYQ30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1966518.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5839..>7305
                     /gene="tacF"
                     /locus_tag="VYQ30_001432"
     CDS             5839..>7305
                     /gene="tacF"
                     /locus_tag="VYQ30_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1966519.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALK"
BASE COUNT         2235 a         1518 c         1226 g         2326 t
ORIGIN      
        1 ttgactttcc tgatagagtt gttcacatct tatttcaatt cactatactt tcccttatac
       61 tcaacgaaaa tcaaagcgca aactaggaag ctagccacag gttgctcaaa gcactgcttt
      121 gaggttgtag ataagactga cgaactcagc tcaaaatact gttttgaggt tgcagatgga
      181 agctgacgta gtttgaagag attttcgaag agtattatat gagaaaaatc ctctactcta
      241 ctaatccact tagaaaaaac gcatattagc aagtgttaaa aacttgctaa tatgcgtttt
      301 attatggaaa tgttgggttc attttctctt aaaattgatt ttttcttaga attctagctt
      361 aggtcatttt taaaattcaa attccgcaag aacatcttgc ccactggtga gcaattttgc
      421 tccttcttga atcaaatgat ggcaaccgtc tgatagtcca tctaaaatgc taccaggaat
      481 agcaaagaca tcgcgtcctt cttccattgc tcgctcacac gtaatgagac tacctgaacg
      541 catcttagcc tctgctacaa tcacaccacg acaaagtcca gcaatgatgc gattacgggc
      601 aggaaaatga aatttcagag gttgttcgcc aggtccatat tcacttagaa ccagatggtc
      661 attgccgatg tagtcttgca agcgtttatt ggctttagga taaaacacat ccagtcctgt
      721 tccaatcact gcaatggttt ttccgccatt ctgaagagct gccatatgag ctgctgtgtc
      781 aatgcccttg gccagaccac tgacaataac cagttcattt tccaagcctt gaatgacttt
      841 ttcaactgac ttagctccct gtttgctaca agcacgactg cccacgaccg ctaccttcgg
      901 gaatttcagg aggtcaagat ttcccttgta aaataaaagt acaggcgcat catatatttc
      961 actcaaatcc caaggataac agtcatctaa aatagagaaa gatggaaatt tttgaaactc
     1021 tttcgacaaa tgcgcatcgt ctatctgaaa ataacgttcc ataaaaacgg ctggattacg
     1081 gcaacctgat atatctgcaa tatcacccaa caaaagctcc tgatcaacat tttcaccgta
     1141 ttctagcact ttcaaaatct gttgattggt caaacctgat ttttttaact tatagatttc
     1201 atagtttgtg attttcataa ataactccat ttctttttct actcatctat ctattcgtaa
     1261 aagttcgtaa aaaatcaaaa aacatccgac tattttgtca gatgttggaa tctttaattt
     1321 tcgtttttaa gaatttcttc taatttacga tagtcttgga cactatcgat ctcataaatg
     1381 ctattgcctt ctaattcttc aacatagaca tctagctctt tgatattatc cttaaccata
     1441 ttgtcccaat agagatcaac aaattcacca cttgcataag ccttgtcgat aaagctgaca
     1501 atcttttctg cagttggagc atcccagaag gatacaccac taaggatgcg acctgccttg
     1561 ctatcaacaa taatgtcttg aaccttgtag tcatctccat agaccaagaa ccattcgttg
     1621 gtacaatctt cacgataaac actaaaataa gtcgaacgtg tcaaatcatt gcggaacata
     1681 tttttaaaga gataattgtc agcatcaata acatagctgt tggccaattc ttcttttaca
     1741 agatagagag agtaaaagtt attgtagtca gcgtatttat cattgaaaac gaggcgaaca
     1801 ccgtattttt cttttaagta atcgaattgt tctttaagat aaccaacaat gatgatgatg
     1861 tcattgattc ctttttcttt gagaaactca atttggtatt caatcaaagg tttttgatta
     1921 acctgaacca aggctttagg ggtattttca gtcataggac gcaagcgagt tcccaatccc
     1981 gctgctaaga taatggcttt cacacgaatc tcctttattc tttaataata atataaactc
     2041 cagcaatgac gacaagtgac gtcataatgg tcagcatatc tagcggtgca cccaaaaaaa
     2101 caactgcaaa caagaccgtc catactacat agctcacgtt caagcctgta gccttggctg
     2161 gttgcaagcg attgatagcg atataataag ccaagtagga aatcatatca aaggctgcaa
     2221 aaacaatcat gagacctagc aattgtccat tggctactgc agtaaatgac tgatgagaga
     2281 agagcacaat cacaagatag gacaagaacg aagtcacttg acggattaag agggcttcga
     2341 tttcactcag ttcactttcc atagcaaagg agctaagaac actctcactt ccccatgcaa
     2401 tagcacaaac caaagcacag agaatcccaa tgtagaagga attaacctgt tcaaccttat
     2461 aggtctgggc aataatccct ccaataatca agacaatccc aaatacagta tttttcgaaa
     2521 tcttgtgctt caaaaagaag aaagccaata gaactgaaat cgcagggtaa atagccgata
     2581 cagatgaagc taaagaactt ccgatatact taactgcata aagattggcc tgcataccga
     2641 tagggcctgc tagcaaggct ccgatgataa cactgacatt gcgaatattt aagaaaattg
     2701 agaggcgaac tttcccttct tttaccaaga gaaaagctag taagataaag atgctcaaaa
     2761 aatcatgagc tgcagccacc acaaagggtg acaaatctgt aaaaatcgaa aagatataag
     2821 cactaaccgt tagacccaag ccccagaaaa tacctgagag aaggccaaaa ggaactccgt
     2881 ttttactttt catctgaacc tccataagaa gccaaacctt taacagctct ttggtaacga
     2941 ttcacaccat agtcaccaaa atcttcacct tgctcttcct tatagacagt ccatagactc
     3001 caaatagtat cttgtaaaat tttataaata gcaatctttt catgagaaac cggtgtttgg
     3061 tcactctcat agtgagataa gaaagtttct tcctcttggg aagtgaattc agactctaaa
     3121 aagagggcag ccaaatccca cattggatca ttcattgatg aatattccca gtcaatcaaa
     3181 taaagtcgtc cttgaggtga ttcgataaag ttttcaggca ccaaatcgat atgacaagat
     3241 tttctgtcaa cacctaagtc agccagtctt ttctctaagg agaagactgc atttctaaca
     3301 gattcatagt tggcataagg aatttgttct tcaatcaagg attcgtattt tttgatttct
     3361 tcaaaaggag caaattctcc tcttaattcc ttagcagacg tatgaatagt ttgtaatatt
     3421 ggagcaattt tgtcgaactt ggtcttgatt gacgttgaat caagcgtaat cgcagattcg
     3481 atatactcat ttactttgat accagcttca atatcaaaaa gataattttt tacatctaag
     3541 cctaaatcct ttagtagttc aagattgtac ttttcatctt gtcgattgat aagcttttct
     3601 gtccctttac caaagaattt aacaatgtat tgcttatttg ttgttttggc caaatagttt
     3661 tgattggtca ttccacccag ttgttcaaca ctgaggactt cctcttcttg actaagtaag
     3721 gaagaaattt tttctttaat gattttctcc acaattaacc tccagtactt atacttccca
     3781 cttaaacact gttttaaagg ctgtgtttaa atcggttgca aagacacgat gaatatcttt
     3841 aatttctctt acaggttctt ctagataaag gatattttta agacgattgg caaatttctt
     3901 gacttccatc atttggatag catttttaaa atcaatgcga ccagaacgag atgacccaac
     3961 caaaatcaag cccttttcta aggcatcgcg agtattgaga ttgactttat attcgctaac
     4021 tcccatcatg agaatcgttc cctgaggacg aatgtagcga atcaagtcat taatagctgg
     4081 tccagtacca tcaccaccac aacattcaaa agcatggtca aaggccaact cttcaggaat
     4141 attatccgta atatagcatt ctttggcaaa tgagaagagt tccaactttt cccaatgacg
     4201 accaataacc acaatctctg attctggcaa agtatagttg ataatattgg caaccacaaa
     4261 agctaaactt ccatctccaa taacggcgat ccgctcccgc ttgctatgag caagagtcaa
     4321 tagacgattc atagcgtgca tgcccacact gacaaactct gtaatggctg caaccgtatc
     4381 ttcaatagca tcataagcca ccacacgatc tttagggaga gaaacaaact ctctcataaa
     4441 gccatcaaat ccactagaca agaaatgggt ccctgtcatg tagttttcat agaattcttc
     4501 atcactctgc ataggagact gattgggaat catgacaact ttttgaccaa cctcgtaggt
     4561 tccggtcggg tcagaaatga cggttccaca tgactcgtga atcattgcca ttggaagctt
     4621 tttattcaaa atcttgggat cacgttttcc ctgatagtaa cgctgatccg catgacagac
     4681 agccatgtag ttgggacgga taaggatatg attctcttgg tcaatagcct cttcctgata
     4741 tttgacattg ataaacttag gcttagttag ttgataaatt tgattaatca ttttactagt
     4801 ctttctcaat catacttttt gcaatcttca aatctgttac ggttgtaatc ttcagatttg
     4861 agtattcacc tttggccaag gccacatctt ttcctttgat cacaaagatt ttacatgcat
     4921 ctgtcaagat ttccttctct tcatcagaaa gagatccata aaggtccatg aagtccttgc
     4981 aacggaatgt ttgaggtgtt tgtccttgat aaaggtgagc acgatttgga atatctgtaa
     5041 taaattgacc attggtactt tcaacgatag tatcaaccgc ttctaccact gtgtccactg
     5101 cgtcatgatt ttgggcaagt tggatattgt cctgaatcat gcgaagtgta ataaatggac
     5161 gaacagaatc gtgggtaaca acgatatcct ctggagtaag cggacgataa gcatcaatgg
     5221 cttcaatgat tttcttaata cttgtattgc ggtcagcacc accctttgta atgatgatac
     5281 gttccttata aagaggaaga tatttatcta caagatcttc tgcatgagaa acccagtctc
     5341 catgaacacc aactacaatt ttttcaatac ttggctccaa gacaaatttt tcaattgtat
     5401 gaatcaaaat aggtcgatta cctagctcta aaaattgttt tggcaagtta ctgatcccca
     5461 tgcgtgtgcc agttccaccg gcaagaattc ctgcataaat catctatttt ctcctttgtc
     5521 ttactaaaaa aacttattct ctctattata ccacaatcta gtcctatcat aaaagaaata
     5581 ctggtcctcc ccttctagaa taggagaatt atgcagttct attattcaaa gaaactagtc
     5641 catttctagt aatgactagg aattctgtag caattactaa taaatggctg cgaaatttta
     5701 gagtccttca gaataatata ctttccttaa agaaaactta aaacttaaga tttgaaactt
     5761 caaagtgatt acttgactta ctgcccttca tttcttatta gctaacttta tgatataatg
     5821 aaaaacgagg taaattgaat gaaaagtata aaattaaatg ctctatctta catgggaatt
     5881 cgtgtcttga atattatttt tcccatccta actggaacct atgtcgcgcg tgtcttggac
     5941 cgaactgact atggttactt caactcagtc gacactattt tgtcattttt cttgcccttt
     6001 gcaacttatg gtgtctataa ctacggttta agggctatca gtaatgtcaa ggataacaaa
     6061 aaagatctta acagaacctt ttctagtctt ttttatttgt gcatcgcttg tacgattttg
     6121 accactgctg tctatatcct agcctatcct ctcttcttta ttgataatcc aatcgtcaaa
     6181 aaggtctacc ttgttatggg gattcaactc attgcccaga ttttttcaat cgaatgggtc
     6241 aatgaagctc tggaaaatta cagttttctc ttttacaaaa ctgccttcat ccgtatcctg
     6301 atgctggtct ctattttctt atttgttaaa aatgaacacg atattgttgt ctatacactt
     6361 gtgatgagtt tatcgacgct gattaactac ctgattagtt atttttggat taaaagagac
     6421 atcaaacttg ttaagattca cctaagtgat tttaaaccac tctttctccc tctgacagcc
     6481 atgttagtct ttgccaatgc caatatgctc ttcacttttt tagatcgcct cttcctcgtt
     6541 aaaacaggga ttgatgtcaa cgttagttac tataccatag ctcagcgaat tgtgaccgtt
     6601 atagctgggg ttgtaacagg tgcaattgga gtgagtgtgc ctcgtctcag ttactatctg
     6661 gggaaaggag acaaagaagc ctatgtttct ctggttaata gaggtagtcg aatctttaac
     6721 ttctttatca ttccactgag ttttggactc atggttttag gaccaaatgc catcctactt
     6781 tacggtagtg aaaaatatat cggaggcggc atcttgacct ctctcttcgc ttttcgtacg
     6841 attatcctgg ccttagatac cattcttggt tcccaaattc tctttaccaa tggctatgaa
     6901 aaacgtatca cagtctatac agtctttgct gggctactca atttgggctt gaatagtctc
     6961 ctctttttca accatatcgt ggttcctgaa tactacttac tgacaactat gttatcagag
     7021 acttctctac ttgttttcta tattattttc atccatagaa aacaactcat ccacttggga
     7081 catatcttta gctatactgt tcgatactct ctcttttcac tttcctttgt agcaatttat
     7141 ttcctgatta atttcgtgta tcctgtggat atggtcatta atttgccatt tttaattaat
     7201 actggtttga ttgtcttgct atcagctatc tcttatatta gtctacttgt cttcacaaaa
     7261 gatagcattt tctatgaatt tttaaaccat gtcctagcct taaaa
//
LOCUS       DAVMEF010000094         7155 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMEF010000094 DAVMEF010000000
VERSION     DAVMEF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.094"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..794
                     /locus_tag="VYQ30_001433"
     CDS             <1..794
                     /locus_tag="VYQ30_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1966520.1"
                     /translation="SKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMAYIQQKRK
                     VGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGGSIQHFNQ
                     YIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVYTFFRRPW
                     HDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFLSSIEQYS
                     FTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            822..2246
                     /locus_tag="VYQ30_001434"
     CDS             822..2246
                     /locus_tag="VYQ30_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966521.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            2311..3399
                     /gene="wecB"
                     /locus_tag="VYQ30_001435"
     CDS             2311..3399
                     /gene="wecB"
                     /locus_tag="VYQ30_001435"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV1966522.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            3562..4431
                     /gene="rfbA"
                     /locus_tag="VYQ30_001436"
     CDS             3562..4431
                     /gene="rfbA"
                     /locus_tag="VYQ30_001436"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1966523.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            4432..5025
                     /locus_tag="VYQ30_001437"
     CDS             4432..5025
                     /locus_tag="VYQ30_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1966524.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            5037..6083
                     /gene="rfbB"
                     /locus_tag="VYQ30_001438"
     CDS             5037..6083
                     /gene="rfbB"
                     /locus_tag="VYQ30_001438"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1966525.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(6145..6999)
                     /gene="rfbD"
                     /locus_tag="VYQ30_001439"
     CDS             complement(6145..6999)
                     /gene="rfbD"
                     /locus_tag="VYQ30_001439"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1966526.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
BASE COUNT         2097 a         1274 c         1473 g         2311 t
ORIGIN      
        1 gctctaaaga tttactgctc ttagttccat tcttgatttt tatttctaaa accttattat
       61 ctgggggtag attggatatt ataaaaattt taattgcgta tgttgtaatg gcctatattc
      121 agcaaaaacg aaaagttggc tgggataagg tcatctccca taaatatatg agacttggtt
      181 ttgtaggctt gatagctggg attcctacct tttactattc tttattctta tctggacgtt
      241 ctacgactag aactgtattt gaaagtattt caacctattt aggaggttcg attcagcatt
      301 ttaatcagta tattcaaaat cctattggag tagctgaggt ttttggcgat gagtcatttg
      361 tagctattat gaatattttg ggtaatcttg gctttgtcaa ttacaatagt accgttcact
      421 tagaatttcg gcagttaggg attactatgg gtaacgttta tactttcttt agaagaccct
      481 ggcatgactt tgggttagtc ggtatgtata tcttttcctt tgttgtcggt gtgttttttg
      541 ctatttttta tttgaaatta agaaagagcc gtgctggttt taaattggat attcatacaa
      601 ttatttactc ttatttcttt tattggattt ttttatcgtc cattgagcaa tactcgttta
      661 caacaatcag tttgtttaca cttgtattta tagtcttggt ttacctaatg gctttcttct
      721 attggaattt ggatttccac agaggaaaac tagtaattaa actctctgac acaagtatta
      781 aatcggaaaa ctgaaaacaa taaaaataga gaggatagtt gatgaatagc aaaattaaaa
      841 atatactaac taatttctct tatgtcattt cttcaaatct tttgacagtc ttgacctctt
      901 ccttggttgt cttaattttc cccaaattaa tgggggtaac agagtacagt tactggcaac
      961 tttatatttt ttatctgacc tatatcggtt ttttccactt gggttggatt gatgggattt
     1021 atcttaaata tggtggctta gaatataata atttagatag aaaacagttt tattctcaga
     1081 tgattctatt ttctagcttc ttaatgatta tctccttgct attgtttggt ttaaatgtat
     1141 tagctgtaac agattcgaat gcacgatata tttataatat gactattata agtatgataa
     1201 tcacaaattt aagaacgctc tacgtgtatg ttttgcagat gacaaaccgc ttgaaggata
     1261 gttccatcat tctactcagt gatcgcgttt tatatgtgct ccttttattc ctgtttattg
     1321 tatttggttg gcatgaatac aaggttatga ttttggcaga tattctaggt cgatcatttt
     1381 ctctcatact ttccttctgg atttgtaaag atattgtgtt tcagcctttg tcaaaattca
     1441 tctttaatat aaaagagtct tttgataata tccgagttgg tatcaattta atgttatcca
     1501 acatcgcaag tagtttgatt atcggtattg ttcgtctagg aatccaatgg aactggaata
     1561 tcgaaacatt cgggaaagta tctctgacct tgagtgtttc caatctattg atgactttca
     1621 ttaatgccat tggactagtc gtctttccgc tattaagacg aacaaaagca gaaaacttac
     1681 ctaagattta ttctaattta agaaacgttt tgatgcttat catgttcgcg attttgctca
     1741 tttactatcc tttaaaaatt gtattagacc tctggttgcc agcctatcaa gatgccttga
     1801 ttttcatgac ccttattttc cctatgtcag tctatgaagg gaaaatggca ttggtcatta
     1861 atacttactt aaaggcatta agaatggaaa gagatatcct tagagtcaat gccttagtaa
     1921 tgttgatcag tatgggagtg accctggtta caacatacct gttaaatagt ttggagctga
     1981 ctgttgtatc gatagttgtt ttgctagctt tgagaagtat catagccgaa ttaattctgt
     2041 ctaaaaaact ggatgtttcg gttaagaagg atattgtatt agaatttctt ttgacgcttg
     2101 tctttatttc ttcaagttgg tatttgccga ttgggctggc agtaattgtc tacacaatag
     2161 cctacggttt atatctctat ttgaaacacg aagatatcaa aacctattta gcttacttta
     2221 aagctagtaa aaaaacatca aattaaaaaa ttatataatg attaagtggt agattctatt
     2281 tctaccgttt tagatattcg ggaggtaatg atgaaaaaaa ttatgttagt attcggtaca
     2341 cgtccggaag ccatcaaaat gtgtccatta gtcaatgagt tgaaaaaaca cgaagatatg
     2401 gaaacaattg tgtgtgttac tggacaacac aaagagatgg ttagtcctgt tttagattta
     2461 tttggtgttg taccagatta tgatttagaa attatgaagg ctaaccaaac cttgttctct
     2521 atcacaacta gtatcttgga aaagataaaa ccagttttag agaaggaaca accagatatt
     2581 gtcctagttc acggtgacac tacgacaact tatgcagcag ccttggcagc attctatttg
     2641 ggaattaaag taggacatgt tgaagctggt ttgcgaacgt acaatttaca aagtccattt
     2701 cctgaagaat ttaacaggca atcgacatca atcattgcaa cttaccattt tgctccaact
     2761 gagttggcta aagaaaatct cttaaaagaa ggtagagaga atgtttatgt gactggaaat
     2821 actgtcattg atgctcttac aactactgtt caagaggatt atacacacac tcatttagat
     2881 ttaaacgcta acaatcgtct catcttattg actgctcata gacgcgaaaa tctcggcgaa
     2941 ccgatgagac atatgtttag agcagttaaa cgagtattga atgaatacga agatgttaaa
     3001 gtcatttatc caattcataa gaatcctttg gtacgtgaaa cagctgcaga gatctttgga
     3061 gatacagaac ggattcaaat tattgaacct ttggatgttc ttgatttcca caacttcatg
     3121 aaccaaagtt atatgatttt aacagattct ggcggagttc aggaagaagc accttcttta
     3181 ggaaaacctg tattggttat gcgcgatact actgaaagac ctgaaggagt agcggcagga
     3241 accttgaaat tagtaggaac tgacgaagaa acaatttatc aaaacttcaa actacttcta
     3301 gatgattctg gagaatacaa aaaaatgagt caggctagca atccatacgg taatggtgat
     3361 gctagtcaac agattgttca gattttgcgt gggatttaag aagattctct aaaagagtct
     3421 aagaggaatc cactccacaa acataaaact cttatgctat catttatgat tgagatagac
     3481 tcataactta atatttttat cctaagaaaa ttcgttcata ttcaatatga atgaaaaaca
     3541 acgaagaaag gttatttcat tatgaaaggt attattcttg caggcggctc aggtacccgc
     3601 ctgtacccac ttactcgggc tgcgtcaaaa cagctgatgc cggtttatga taaacctatg
     3661 atttattatc cgttgtcgac attaatgttg gctggaatta aagatatttt gattatctca
     3721 actcctcaag atttgccccg ttttaaggac ttgctcttgg atggttccga atttgggatc
     3781 aagctttcct atgcggaaca acctagtccc gatggacttg ctcaggcttt tcttatcggt
     3841 gaagaattta tcggtgacga tagcgttgcc ttgattttag gcgacaatat ctaccatggt
     3901 cctggtctga gcacaatgct tcaaaaagca gccaagaaag agaaaggtgc gactgttttt
     3961 ggctaccaag tgaaggatcc agagcgtttt ggtgtggttg agtttgatac agacatgaat
     4021 gctatttcta tagaagaaaa accggagtat cctcgctcca actatgcagt gacaggactg
     4081 tatttctatg ataatgatgt tgtggagatt gctaaacaga tcaaacctag tgctcgtggc
     4141 gagttagaaa ttacagacgt taacaaggct tacctaaatc gtggtgacct ttctgttgag
     4201 ctgatggggc gtggttttgc ctggttggat acgggaaccc atgaaagctt gctagaagct
     4261 tctcagtata ttgaaacggt tcagcgtatg cagaacgttc aagtggcaaa tctagaagaa
     4321 atttcctatc gtatgggcta cattagtcgt gaagacgtgc tggaattggc tcagcctctt
     4381 aagaaaaatg aatacggaca atatttgctc cgtttgattg gagaagcata gatgtcagat
     4441 aatttttttg gaaagacact tgtggtacgc aagattgatg ctataccagg actgctagag
     4501 tttgacattc ccgttcatgg agacaatcgt ggttggttta aggaaaattt ccagaaggaa
     4561 aagatgctac cgcttggttt tcctgaaagc ttctttgctg cagggaaact gcaaaataac
     4621 gtcagctttt ctcgcaaaaa tgttcttcga ggactccatg ctgaaccttg ggacaagtat
     4681 atctctgttg cagacgatgg gaaggtttta ggatcttggg tagatttgcg tgaaggcgag
     4741 acctttggaa atgtttacca gacagagatt gatgctagca agggaatttt tgtccctcgt
     4801 ggcgtagcca acggttttca agtcctctca gatacagttt cctatagcta tctggtcaat
     4861 gactactggg ctcttgaact caaacccaaa tatgcctttg ttaactatgc tgacccagca
     4921 ttgggaattg agtgggaaaa cctagcggaa gctgaggttt cagaggcaga caaacaccat
     4981 cctctattaa gggatgtcaa accacttacg aaagacgagt tgtaaaagga aacattatga
     5041 ctgaatacaa aaaaattatc gtgacaggtg gagctggttt tatcggttct aactttgtcc
     5101 actatgttta caataacttt ccagatgtcc atgtgacagt gctggacaag ctgacttatg
     5161 caggtaatcg tgccaatatc gaggaaattt taggcgaccg tgttgagttg gttgttggag
     5221 atattgctga tgcagccttg gtagacaagt tggcggctga agcggatgct atcgttcact
     5281 atgcggcaga aagccacaat gataactcgc tcaatgaccc gagtccattt atccacacca
     5341 actttatcgg aacttacaca cttttagaag cggctcgtaa atacgacatt cgtttccacc
     5401 atgtatcgac tgatgaagtc tatggtgacc tgcctctgcg tgaagatttg ccaggtcatg
     5461 gggaaggcct aggtgagaaa tttaccgctg aaaccaagta caatccaagc tcgccttact
     5521 catcaaccaa ggctgcttca gacttgatcg ttaaagcttg ggtgcgctca tttggtgtta
     5581 aagcaacgat ttctaactgt tcaaacaact atggtccata ccagcatatc gagaagttca
     5641 ttccgcgcca gattacgaat atcttgagcg gtatcaagcc aaaactctac ggagaaggta
     5701 agaatgtgcg tgactggatt cacaccaatg accattcatc aggcgtttgg acgattctga
     5761 ccaagggtca aatcggtgaa acttacttga tcggtgctga cggtgagaag aacaacaagg
     5821 aagtgttaga gctcattctc aaggagatgg ggcaacccgc tgacgcttat gaccatgtga
     5881 ccgatcgagc tggtcacgac ttgcgttatg cgattgatgc tagcaagctc cgtgatgagt
     5941 tggggtggaa gccagagttt accaactttg aagcaggcct caaagagacc atcaagtggt
     6001 acacagataa ccaagactgg tggaaatctg aaaaagaagc agtagaggct aactatgcta
     6061 aaacgcaaca agtgattaaa taataagata ctaaaaaagc aagagacctc gaggtctctt
     6121 gcttttctag tatgtagtaa gcttttattt tcttacttct tgtttataaa actctttaag
     6181 ggcatcttgc caggttggga tgacgaaacc tgttgccttt gcctttgcca aactcatagt
     6241 tgagttgaga ggtcgtttag ccttagcggg gaacttgcta gagtctactg gcactacttc
     6301 aacatcactg tccttaagaa tctcgctagc aaagtcatac caagtggtgt cttcagctgc
     6361 gtcatttgat aagtgatagt agccatactc tttttgattt tcagtcacat aggtcatgaa
     6421 ttcggccaag gtccgtgtcc aagttgggcg accgtattgg tcgttgacca ctgtgagcgt
     6481 tttatgggtt ttggctaggc tttgcatggt aaagacaaag ttttttccat aatttccaaa
     6541 aacccaagca gtacgaatga tgtaatgctg tgacgtaagg ttttcaacaa gttcttctcc
     6601 cattcgcttg gtacgtccgt actctgtttg cggatcaggt atgtcatcga cttcccactc
     6661 ttgtcctact ggtttctttc catcaaagac gtagtctgtt gagatataga ccagaatagc
     6721 tccgtatttc tcagaggcct tggctacatt ttcagttcca gttacgttga tggcaaaatc
     6781 cagttctttc ccttcatctt cggctgcatc aacagcagtg taggctgcac aatgatagac
     6841 tagagttggc ttaacctcag caaagacttt ttcaaccatt tcagaattag tgatatccat
     6901 ttcggtcaca tccactgcaa catagtccac atttcgctca tctagtaaat aacgtagttc
     6961 ggtaccaagt tgaccatttg cacctgtaat taagatcata tttttctcct tgatagttct
     7021 tatacttaat tatatcaaaa atatagaaaa atcggttttt agtgaaccct tttttggtat
     7081 aaactttgtt agtgaagcgc aagagagccg aatagttatt actttagaat atccttaaaa
     7141 gtatttttca gaatt
//
LOCUS       DAVMEF010000095         7131 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMEF010000095 DAVMEF010000000
VERSION     DAVMEF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.095"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            59..976
                     /locus_tag="VYQ30_001440"
     CDS             59..976
                     /locus_tag="VYQ30_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1966527.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MFNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            987..1877
                     /locus_tag="VYQ30_001441"
     CDS             987..1877
                     /locus_tag="VYQ30_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1966528.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            1906..3522
                     /locus_tag="VYQ30_001442"
     CDS             1906..3522
                     /locus_tag="VYQ30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1966529.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            3532..4851
                     /locus_tag="VYQ30_001443"
     CDS             3532..4851
                     /locus_tag="VYQ30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1966530.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(5059..5131)
                     /locus_tag="VYQ30_001444"
     tRNA            complement(5059..5131)
                     /locus_tag="VYQ30_001444"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5096..5098),aa:Lys,seq:ctt)
     gene            complement(5396..6667)
                     /locus_tag="VYQ30_001445"
     CDS             complement(5396..6667)
                     /locus_tag="VYQ30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1966531.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFNFPIELDEDALDFIATSTNGDLRSTFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6937..>7131
                     /locus_tag="VYQ30_001446"
     CDS             6937..>7131
                     /locus_tag="VYQ30_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966532.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAY"
BASE COUNT         2201 a         1278 c         1514 g         2138 t
ORIGIN      
        1 acagcgtcaa gcgctatctt ttctagaaaa aatgaaacgt ttcaaaaagg aggttgctat
       61 gaatagcaaa gcgaagcaag tttctctttg ggaaagaatc aagaaacaaa aactcttgtt
      121 attgatgact gtccccggtt tagttttaac ctttatcttt aaatacatcc ctatgtatgg
      181 ggttttaatc gcatttaaag attacaatcc tttaaaagga attttaggga gtgattggat
      241 tggtttttct gagtttacaa aattcatatc ctctcccaac tttggtatct tgttagccaa
      301 cacattaaaa ttaagtatct atggtttatt gcttggcttt ttaccaccaa tcattcttgc
      361 tattatgttc aatcaactct tgagtgaaaa agtcaaaaaa cgaattcagc tcattttata
      421 cgcaccaaac tttatctcag tcgttgttat tgtcggtatg attttcctct tcttttcagt
      481 gggaggacca atcaacaatt ttctttctat gtttggaatg aaggctgact tcttgacaaa
      541 tccagacttc tttagaccct tatacatctt tagtggtatc tggcaaggaa tgggctgggc
      601 ttcaacgctc tacacggcaa cattggtaaa tgtagatcca gccttagtag aagcagcccg
      661 actggatgga gccaatatct tccaacgaat ctggcacatt gatattccag ctcttaagcc
      721 tattatggtt atccaatttg ttttagctgc aggtggaatt atgaatgtcg gatatgaaaa
      781 agcattcttg atgcagacat cgttaaattt gccaacttct gaaattatct cgacatatgt
      841 ctataaagtt ggtcttgtat caggagacta ttcttactca acagcggttg gtttgtttaa
      901 tgcagtgatt aacgtagtat tgcttgttgc agttaaccaa atcgttaaac gcatgaataa
      961 tggtgaagga atttaaggag gaaagtatga aaaattcgat tatggataca aaatttgata
     1021 gacgtatctt actcttaaat aaaatcatta ttgtctttat cgttttgatg actttgcttc
     1081 ctttacttta tatcgtcgta gcatccttta tggatcctaa ggttctggtt agtagaggga
     1141 ttagctttaa tccagccgat tggactgtag aaggttacca gcgtgtattc agtgaccaat
     1201 ctattctaag aggttttatc aattctctac tatactcttt tggatttgca gctttaacag
     1261 tattgctatc tgtgtttaca gcttatcctc tttctaagaa agacttggtt ggacgtcgtt
     1321 ggattaacta cttcttgatt gtgactatgt tctttggtgg tggtttagtc ccaacttact
     1381 tgctcgtaaa agaattggga atgctcaata ctccatgggc tatcattgtt ccaggtgctg
     1441 tcaacgtttg gaatattatt cttgctaggg cctatttcca aggattgcct gaagaattag
     1501 ttgaagctgc tgtcattgat ggtgcaaatg atttacagat tttcttcaaa atcatgcttc
     1561 ctcttgcaaa accaattatg tttgttctct tcctttatgc ttttgtagga cagtggaact
     1621 catactttga tgcaatgatt tatatcaagg atccaaactt ggaaccattg caacttgtac
     1681 ttcgtaaaat tctcattcag agccaaccag gtcaagacat gattggagca caagcggcta
     1741 tgaatgaaat gaaacgttta gctgaattga ttaaatacgc aactattgtc atttctagct
     1801 tgccattgat tgttatgtat ccattcttcc aaaaatactt tgataaagga attatggctg
     1861 gttcacttaa aggataaaaa aagaaaaaat aaaaggagtt ttctcatgaa attcaaaaca
     1921 ttctcaaaat cagcagtttt gttgacagct agtttagcag tacttgcagc ctgtggctca
     1981 aaaaatacag cttcaagtcc agattataag ttggaaggtg taacattccc gcttcaagaa
     2041 aagaaaacat tgaagtttat gacagccagt tcaccgttat ctcctaaaga cccaaatgaa
     2101 aagttaattt tgcaacgttt ggagaaggaa actggcgttc atattgactg gaccaactac
     2161 caatccgact ttgcagaaaa acgtaacttg gatatttcta gtggtgattt accagatgct
     2221 atccacaacg acggagcttc agatgtggac ttgatgaact gggctaaaaa aggtgttatt
     2281 attccagttg aagatttgat tgataaatac atgccaaatc ttaagaaaat tttggatgag
     2341 aaaccagagt acaaggcctt gatgacagca cctgatgggc acatttactc attcccatgg
     2401 attgaagagc ttggagatgg taaagagtct attcacagcg tcaacgatat ggcttggatt
     2461 aacaaagatt ggcttaagaa acttggtctt gaaatgccaa aaactactga tgatttgatt
     2521 aaagtcctag aagctttcaa aaacggggat ccaaatggaa atggagaggc tgatgaaatt
     2581 ccattttcat ttattagtgg taacggaaac gaagatttta aattcctatt tgctgcattt
     2641 ggtatagggg ataacgatga tcatttagta gtaggaaatg atggcaaagt tgatttcaca
     2701 gcagataacg ataactataa agaaggtgtc aaatttatcc gtcaattgca agaaaaaggc
     2761 ttgattgata aagaagcttt cgaacatgat tggaatagtt acattgctaa aggtcatgat
     2821 cagaaatttg gtgtttactt tacatgggat aagaataatg ttactggaag taacgaaagt
     2881 tatgatgttt taccagtact tgctggacca agtggtcaaa aacacgtagc tcgtacaaac
     2941 ggtatgggat ttgcacgtga caagatggtt attaccagtg taaacaaaaa cctagaattg
     3001 acagctaaat ggattgatgc acaatacgct ccactccaat ctgtgcaaaa taactgggga
     3061 acttacggag atgacaaaca acaaaacatc tttgaattgg atcaagcgtc aaatagtcta
     3121 aaacacttac cactaaacgg aactgcacca gcagaacttc gtcaaaagac tgaagtagga
     3181 ggaccactag ctatcctaga ttcatactat ggtaaagtaa caaccatgcc tgatgatgcc
     3241 aaatggcgtt tggatcttat caaagaatat tatgttcctt acatgagcaa tgacaataac
     3301 tatccaagag tctttatgac acaggaagat ttggacaaga ttgcccatat cgaagcagat
     3361 atgaatgact atatctaccg taaacgtgct gaatggattg taaatggcaa tattgatact
     3421 gagtgggatg attacaagaa agaacttgaa aaatacggac tttctgatta cctcgctatt
     3481 aaacaaaaat actacgacca ataccaagca aacaaaaact agaggttgat tatgggagat
     3541 aagaaataca cagtagaaaa agccaatcgt tttatagcag aaaataaaca tctcgttaat
     3601 actcaatata agcctgaaga acatttttca gctgagattg gttggatcaa tgatccaaat
     3661 ggatttgtct attttcgtgg agaataccat ctcttttatc aattctatcc atatgatagt
     3721 gtttgggggc ctatgcactg gggacatgct aaaagtaagg acttggtgac ttgggagcac
     3781 ttgccagtgg cacttgctcc tgaccaagat tatgaccgaa atggttgctt ctcaggctct
     3841 gccattgtca aggatgatcg cctctggctc atgtatactg ggcatatcga agaagaaacc
     3901 ggtgtccgcc aagtgcaaaa tatggcattt tcagatgacg ggattcactt tgaaaagatt
     3961 tcccaaaatc cagttgcaac tggagcagac ttgccagatg agttgattgc tgctgatttc
     4021 cgtgatccaa aactctttga aaaagatgga cgctattact ccgtagtagc tgccaaacac
     4081 aaggataatg tgggctgtat cgttctacta gggtccgata acctagtaga atggcagttc
     4141 gaatccatct ttttaaaagg gggagaacac caaggcttta tgtgggaatg cccagattac
     4201 ttcgagttag atgggaaaga ttgccttatt atgtcaccca tgcgttatca gcgtgaggga
     4261 gactcatatc ataacatcaa ctcatcgctt ttgttcacgg gtaaggtaga ttggggagaa
     4321 aaacgtttta tcccagaatc agttcaagaa attgatcatg gccaagactt ctatgcgcct
     4381 caaacattgt tggacgatca aaatcgtcgt atcctgattg cttggatgca gacatggggg
     4441 cgtacccttc caacccatga ccaagaacac aagtgggcat gtgccatgac tttacctaga
     4501 attctaagat tggaagatgg caaactaaga caattccctg ttaaaaaagg ccaatatcaa
     4561 atccaaatag ataaagattg tcattaccac ttaggaaatg atacagatta tcttgaattt
     4621 ggttatgaca gtaatgtgca gcaagtttac attgatcgta gccatcttat tcaaaaaatt
     4681 ctaggtgaag aagaacagga cactagtcga cggtatgtag atattgaagc taaagaattg
     4741 gaagttgttc tagataaaaa ttccatcgag atttttgtca atcaaggtga agcaagcttg
     4801 actgcaactt attacttaac ggtgccagct gagctatcac gaattgatta aaaattaagt
     4861 tatttctcct aaagaaaaag ttctctttct aaaatagtgg aaagaggact ttttgtgttt
     4921 tgggtatata agcttagttt atggtatttg taaaattggt gttggattat gatttaagct
     4981 agttttctaa agaatttgaa aaaaatttta tttaagcaaa aaaaccttgg ttccaaggct
     5041 tttcctgttg tatttagatg ccccctacag ggatcgaacc tgtgacccac ggattaagag
     5101 tccgctgctc tgccagctga gctaaggagg caaagaaaaa agctgtattg gtgccgaaac
     5161 ttcacggttt gtattgaacc cgcgcaatta agcaggtggg caactcgctc taactgaagc
     5221 tgtttccgtg tgagacggcc tacatgctgt tagaagactt ttgtttccct aataatacaa
     5281 aaaatagtcg gtcaacactt aagtgtgaag tcgtacacca cagcgtttct atacttatat
     5341 gataccactt tttcaaaaaa aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga
     5401 tttttcgcaa acggtcgata gcttcctttc tttgagccaa agcccgttca tatttaccag
     5461 tatcttctgc ttggaaatag tgatgattac gaattttttc tggcagatag tcttgcttga
     5521 cccaatttcc aggatagttg tgtggataga gatagtcttg ggcattcccc agttccttgc
     5581 ttccactgta gtgcccatca cgtaggtgtc gcggaatagg caagtgccct gatgttttga
     5641 ggtcagcaag tgccttatcc atagctacat aggctgagtt ggattttgga gaaagtgcca
     5701 aatcaatcac gacattggca atgagaatgc gggcttctgg gaacccaatc ttctgggcag
     5761 catccagagc agtcacggta tgaatctggg cctcaggatt ggctaagccg atatcttcat
     5821 aggcgataac agtcaagcga cgagcgagac taggcagatc cccagcctca atcaagcggg
     5881 cagcatagtg gagactggca tccacatctg agccacggat agacttttgc agggcagata
     5941 gaacatcata gtgaccgtct ccatccttgt ccatagtgat gtagcttctc tgaagactat
     6001 tttccatgat gtctaaggtg atgtggcgaa tgccctcgtc attctcaggg gtagagagaa
     6061 cagccaagtc cagtgagtta aaggtagagc gaaggtctcc gtttgtagag gttgcgatga
     6121 aatccagcgc atcctcatct agttctattg gaaaattaaa accacgttca gggttactta
     6181 gagctatctg cagggcctct ttgacgtctt ggttagacag aggttccaac tcaaaaattt
     6241 gaacacggct acgaatggca ggagtgacgg aaaagaaagg attttcagtc gttgctccaa
     6301 tcatgatgac cagtccactt tccaagagag gcaagaggaa gtcttgcttg gtcttatcta
     6361 gtcgatgaat ttcgtctagc aataggacga gaccaccaga aaatttagct tcttccgaga
     6421 tttcttgcag tcgcttttta ctatcaactg tcgcattgaa agttcgaaag gcatacttgg
     6481 tcgttccagc gatggcagag gcaatactgg ttttgccgat tccaggaggg ccatatagaa
     6541 tcatggagga caggcggttg gcttccacca tgcggcggat gatttttcca ggtccgacca
     6601 gatgctcctg accgatgacc tggtcgatgg ttttagggcg catgcgaagc gcgagattgt
     6661 ctggcatagc agtcctttct aacgtggatt tgagtagaat gactcatcta ctatatctta
     6721 ctatgtccat tctagcctat tagttgtgat atttcaatgg ttttgtaata atcagggagt
     6781 gagatagaat cttttttact atttaattta atgaggagag gtatcagcat gagagatttg
     6841 attttcctta aagctcaaaa taggatagtt gagcctaaag aagtggaagt gctgacttga
     6901 gaaataatat tgctaaatgt cattgtatga actataatga aaagagaggt gataagcaat
     6961 ggaaatgatg gaccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt
     7021 cttagtgatc aagttatttc agggaaaaga aaacttttct tccgggaaga tactccaaaa
     7081 gagattttag atttgtacga aaacattaaa tctaaacttg attttgctta c
//
LOCUS       DAVMEF010000096         7103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMEF010000096 DAVMEF010000000
VERSION     DAVMEF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.096"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            166..504
                     /locus_tag="VYQ30_001447"
     CDS             166..504
                     /locus_tag="VYQ30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1966533.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            515..874
                     /locus_tag="VYQ30_001448"
     CDS             515..874
                     /locus_tag="VYQ30_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1966534.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            981..1624
                     /gene="pcp"
                     /locus_tag="VYQ30_001449"
                     /pseudo
     CDS             981..1624
                     /gene="pcp"
                     /locus_tag="VYQ30_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(1652..2509)
                     /locus_tag="VYQ30_001450"
     CDS             complement(1652..2509)
                     /locus_tag="VYQ30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1966535.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            2639..3781
                     /gene="tgt"
                     /locus_tag="VYQ30_001451"
     CDS             2639..3781
                     /gene="tgt"
                     /locus_tag="VYQ30_001451"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1966536.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            3882..5699
                     /locus_tag="VYQ30_001452"
     CDS             3882..5699
                     /locus_tag="VYQ30_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1966537.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIVGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            5852..7003
                     /gene="nagA"
                     /locus_tag="VYQ30_001453"
     CDS             5852..7003
                     /gene="nagA"
                     /locus_tag="VYQ30_001453"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1966538.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPYTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
BASE COUNT         2085 a         1263 c         1644 g         2111 t
ORIGIN      
        1 tcttatttca tttcactata ctttatatta taattgactg gtcaactatt gataaatcaa
       61 taaaaagaga ggggaagaaa tgctagagat tcaagattta ctgtatcaac tccgcttgtc
      121 tgagcaagcg agtacgcaat tgtttgaaaa aaggcttgga ttagtttgac acggtatcag
      181 attttactgt ttttgctgga gcattctctt tgtaaccaaa tggcggttca ggagcgtttg
      241 aaaattgatc aggctgcttt gacacggcat ttcaagattt tggaaacgga aggtttggtg
      301 gagcgtcatc gtaatcctga aaatcagcgg gaagtgttgg tagaggctgc gaagtatgcc
      361 aaggagcagt tagtggtgaa tccccctctg caacatatca aggttaagga agagatggaa
      421 agtatcttaa cagagtttga gagaacagaa ctcagccgtt tattaaataa attggttttg
      481 ggtattgaaa atatagaaat ttaaggagaa atagatgtca attattttaa caacgatcgt
      541 tgctttggag catttttaca ttttttattt ggaaagtatt gccacgcaat cagatgcgac
      601 tagtcgtgta tttaatatgg aaaaggaaga attggctcat ccgtcagtaa gttcattgtt
      661 caaaaatcaa ggaatttata aggatctgct aggagtcttt ctcttgtatg gcatttattt
      721 ctcacagaat ttagaaattg tgactatttt tgtcttattt gtgattggtg ctgcgactta
      781 cggctcttta acagcggata aaaaaattat tttgaaacaa ggtggatcag ctattttggc
      841 cttgattagt attttactct ttaaatacac ttgaaggtcg attctaatct cgctaatcct
      901 ttttaatcca gaataaggga aatatgttat acttgttttt aagaaaaaag tctcattgaa
      961 ttggttttga ggagttagaa atgaaagtat tagtgacagg ttttgagccc tttggagggg
     1021 aaaagggcaa tccagctttg gaggccatta aaggtttacc agctgaaatc catggtgctg
     1081 aggtccgttg gctagaggtg ccgacagttc ttcacaaatc tgctcaagta ttggaagaag
     1141 agatgaatcg ttatcaacct gactttgtcc tttgtattgg gcaagctggt ggaagaacta
     1201 gtttgacacc tgaacgagtg gccattaatc aagacgatgc acgcatttct gataacgaag
     1261 ataatcaacc gattgaccgt cccattcgcc cagatggtgc ttcggcctac tttagtagtt
     1321 tgccgattaa agcgatggtt caagctataa aaaagaagga ttaccggcct ctgtttccaa
     1381 tacggcaggg acttttgtct gcagccattt gatgtatcag gctctctatt tggtagaaaa
     1441 gaaatttcca tatgttaagg caggttttat gcatattcct tatatgatgg aacaggtggt
     1501 gaacagaccg actactccaa ctatgagttt agtggatatt cggcgaggga tagaagcagc
     1561 aatcggcgct atgatagaac atggagatca ggaactcaag ttggtaggcg gagaaactca
     1621 ttgatagaaa aaagcttgag gggaaaaacc ttcaagcttt tggacgtttt cgagccaata
     1681 ctgctcggta aaacataatt ttagtgcatt ggatataagg taggagtgaa aaactagcaa
     1741 tgccaaaggt aatccaattg aggaagtacc aaggaagaag ctgtaaatct aggacaaagc
     1801 gctggaactt gtagcccttc ataaaggaac ggctagtttt taggattcgt cttggtggga
     1861 cctgtcctag gtctagacta taacagagaa gaaattccac ctgtgaatag gcataatact
     1921 gtggaatata gaggatattt cctacaatga tcaagatgag acttgcaaga aagtagagtc
     1981 caaagaccat gaggaaacgc tcggtttcaa ctgatgagag atctagattt ggaaactcag
     2041 gatgtagggt gacgaatttt ttggctaaaa agctactata aaagaggagg taaatcccaa
     2101 gtaaattagg gatactccat aaaaagagat agaaacgttt gagaagtagg gtcaaaaagg
     2161 tttgagaaaa gcgctcctca tcaaagagag ctaggctgtt ttttacagat ggctccgttt
     2221 tagaatcttt catgagtgtc agtgttgcat agacggaact ggtcaaaaga atagtcccga
     2281 taaaggagac tagtagagga aagaggtagg cttgaagtat ttggccaagt atgctgaaaa
     2341 atggctgttc taaaacagtc ccgtggatcc gagataaggg attaagaaaa ccagataaga
     2401 tgaccagcat actgggaagg atatagagga gaaagagacg gggggtgtca gcctgaaaat
     2461 gttttgactc ctgacgaatt gtttttaaat caatttttgg atagttcatt ctcttattat
     2521 accatagttc ttatacatag ttcgtgacag ttcctacttt ttttgataaa atcatacagt
     2581 gtgtccttgg gcacactgta tgaactggga ctgtctttcc cagcttcgga ggtaaaaaat
     2641 gtcagattca ccaatcaaat atcgtttgat taagaaagaa aaacacacag gagctcgtct
     2701 aggagaaatc atcactcccc acggtacctt tccgacacct atgtttatgc cagttgggac
     2761 acaagccact gtcaaaactc agtcacctga agaattgaag gagatgggtt cgggaattat
     2821 cctatcaaac acctatcatc tctggcttcg ccctggagat gaactcattg cacgcgctgg
     2881 tggtctccac aagttcatga attgggacca gcctatcttg acagatagtg gtggttttca
     2941 ggtttattct ttagcagata gccgtaatat cacagaagaa ggagtaacct ttaaaaatca
     3001 tctaaatggt tctaagatgt tcctatcccc agaaaaagcc atctctattc agaataatct
     3061 gggttcagac atcatgatgt cctttgatga atgtcctcag ttttatcaac cttatgacta
     3121 cgttaagaaa tcgatcgagc gtaccagccg ttgggctgag cgtggtttga aggctcaccg
     3181 tcgtccacat gaccaaggtt tgtttggaat tgtgcaaggt gcaggatttg aagaccttcg
     3241 ccgccaatca gctcatgatc ttgtcagcat ggatttctca ggctactcta tcggtggttt
     3301 ggcagtggga gaaacccatg aagagatgaa tgcggttttg gactttacaa ctcaactgct
     3361 gcctgaaaat aaacctcgtt atctgatggg tgtgggagcg ccagatagct tgatcgatgg
     3421 ggtcattcgt ggggtggata tgtttgactg tgtcttaccg actcgaattg ctcgtaacgg
     3481 gacttgtatg accagtcaag gacgtttggt tgtgaaaaat gcccagtttg ctgaggactt
     3541 tacgccactg gatcctgagt gtgattgcta cacatgtaat aactatacac gcgcttacct
     3601 tcgtcacctg ctcaaggctg atgaaacctt tggtatccgc ttgactagct accacaatct
     3661 ttacttcttg cttaacctga tgaagcaagt gcgacaagcc atcatggatg acaatctctt
     3721 ggaattccgt gagtattttg tggaaaaata tggctataat aagtcaggac gtaatttcta
     3781 aaatggaatt gatataagct aaaaatccta agttttctct taggattttt cttctttttt
     3841 tgatagaata aagtgtacaa tgaaaggaag aataaactcg tatgcgcatt aaatggtttt
     3901 ccttggttag gattacaggt ttacttttgg tactcttgta tcacttcttt cagacgatct
     3961 ttcctggagg atttttcggg gtagatgtct ttttcacatt ttcaggtttc ctgattacag
     4021 ctctactcat tgaagaattt tctaaaaacc atgagattga tttgatagga ttttttagaa
     4081 gacgctttta tcggattgtg ccacctgtgg ttttgatggt cttggtgacc atgcctttta
     4141 ctttcttggt tcgccaagac tatgttgctg gaattggtgg ccagattgcg agcgtcttag
     4201 gctttatgac caacttctat gaactcctaa caggtgggag ttatgaatct cagttcattc
     4261 ctcatttgtt tgttcataat tggagtttgg cagttgaggt tcactactat attctttggg
     4321 gattggcagt ttggttctta tccaaacaag ctaaatcaaa tggtcagttg aaggggatgg
     4381 tctttctctt atctgctgtt gccttcttga tcagtttctt ctccatgttt attggtagtt
     4441 ttctagtgac ctcttattcc tctgtttatt tctccagttt aactcatgtc tatccattct
     4501 ttttgggaag tatgttagca actattgtag gcgttcgtca gacgacttcc ctcgtcaagc
     4561 agttggataa aatctgggat ttacgaaaga ctttggtagt ttttggagga ggctttggtt
     4621 tcctagttct tttgactttc tttgtcaaat tcacttatct ttttgcctat cttatcggct
     4681 tcttgcttgc cagtcttgca gctcttgcca tgattctggc ggcgcgtgtc ttacatgaaa
     4741 agacacatca tatacaggag ccgaagatta tcagcttttt agcggatact agctatgcgg
     4801 tttatctttt ccattggcct ttctatatca ttttctcaca gttgacatca aatcttcttg
     4861 ctgtattact gactctgatt tgttcttatg gctttgccag tctgtcattc tatgtattgg
     4921 aaccttggat tgtaggcaag aacacaccta ttttacaaac tcttcgtccc ctgccttata
     4981 ttcacgcaat tcttgcagca ggtacaggaa tcttgacctt cattgtctgc atggtgacct
     5041 tgttggcacc acaagtggga gcgtttgaga cagacttgac tgtcaatggc ttgaagcaag
     5101 ctgcaacaaa tattggccag accaaggtga tggcagaacg ggcagatgca aacagtttgg
     5161 gaattgctga tggcactatg ttaattggtg actcagtggc tttaagggca aatacagcac
     5221 tacagacagc tcttcctgga gcacagatta acgcgcaggt cagcgtaaca accaagaccg
     5281 caaatgaaat catgctaaat aatagccaga ataaattttt acctaagacg gtggtcattg
     5341 cgactggggt aaataatcct gagaattaca aggatgactg ggacagtatc gtgaaaaatc
     5401 ttcctaaggg acaccatatg attttggtga ctccttatga gggagataag acaaaagaga
     5461 cctatgccat cgttgagaag gctgctgcct atatgagaga attggcagag aagacacctt
     5521 acattacgat agcagattgg aatcaagttg cgaaagagca tccagaaatt tgggcaggaa
     5581 cagaccaggt tcatttcggg agtgagagta gcactatcga agcaggagca aaattgtatg
     5641 cagatacgat tgccacagct ttgcagacag ctcaagataa gccggttaaa tcaaaataac
     5701 ttgtattaaa aaagagaaga gttggagaaa tccagctctt ttaaaatatc tccaaaaaat
     5761 aggtctatac catttacaaa taaaaaagaa aggtttataa tgtaattgac ataataaatt
     5821 gtagaatcaa tcttttaagg aggttaacat tatgcctaac tatattaaag cggatcagtt
     5881 tttctaccca cacggagttc gtcgaggtgg ttacttggaa cttgtggacg gcaagtttgg
     5941 gaaacatgta gagcagattc ctgaaggggc tgaggtgatt gactatacag gttatagcat
     6001 tgccccaggt cttgtggata ctcatattca tggatatgca ggtgtagatg tgatggacaa
     6061 caacattgaa ggtacattgc atactatgag tgaaggactt cttagtaccg gtgttaccag
     6121 tttcttaccc acaactttaa cagccactta tgagcaattg cttgcagtca ctgaaaatct
     6181 tggaaaccat tataaagaag caacaggtgc taagattcgt gggatttatt atgaaggtcc
     6241 atatttcaca gaaactttta agggggcaca aaatccaact tatatgagag acccgggtgt
     6301 tgaggagttt cattcttggc aaaaagcggc aaatggcttg cttaataaaa ttgcccttgc
     6361 accagaacgt gatggggtgg aagactttgt tcgtacagtt acgggcgaag gtgtgacggt
     6421 tgctcttgga cattcaaacg cgacttttga tgaagccaaa aaagcagtcg atgctggagc
     6481 gagtgtttgg gtgcatgcct acaatggaat gcgtgggttg actcaccgtg aattgggtat
     6541 ggttggagcc atgtaccaat tgccatatac ctatgcagag ttgatctgtg atggtcacca
     6601 cgtagatcca aaggcttgcg aaattcttat caaacaaaaa ggaacagaaa acatcgctct
     6661 tatcacagac tgtatgacag ctgggggatt ggaagacgga gattatatgt tgggagaatt
     6721 cccagttgtc gttgcaaatg gaactgcacg cctcaaatcg acaggtaact tggcaggttc
     6781 tatcctcaaa ctcaaagatg gtttgaagaa tgtggtcgaa tggggaattg cgaatccgca
     6841 tgaagcagtc atgatggcca gcttcaaccc agctaaatcc gttcacatcg atgacgtctg
     6901 tggccaaatc cgtgaaggct acgacgctga cttcatcgta ttagataaag atttggaatt
     6961 ggtagcaacc tacctagatg gtgtgaaacg ttatcaagca taagagagaa ggcagaagtt
     7021 agagactagc ttctgctttt ttatacaggg tactttactg gtaaataaaa agttaaaaaa
     7081 atcacaaaaa aagcttgaag aaa
//
LOCUS       DAVMEF010000097         7051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMEF010000097 DAVMEF010000000
VERSION     DAVMEF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.097"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..828
                     /locus_tag="VYQ30_001454"
     CDS             4..828
                     /locus_tag="VYQ30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1966539.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            850..1589
                     /locus_tag="VYQ30_001455"
                     /pseudo
     CDS             850..1589
                     /locus_tag="VYQ30_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1602..2768
                     /locus_tag="VYQ30_001456"
     CDS             1602..2768
                     /locus_tag="VYQ30_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1966540.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     ATTFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            complement(3259..4929)
                     /locus_tag="VYQ30_001457"
     CDS             complement(3259..4929)
                     /locus_tag="VYQ30_001457"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1966541.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            5159..5848
                     /gene="coaB"
                     /locus_tag="VYQ30_001458"
     CDS             5159..5848
                     /gene="coaB"
                     /locus_tag="VYQ30_001458"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1966542.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            5860..6411
                     /gene="coaC"
                     /locus_tag="VYQ30_001459"
     CDS             5860..6411
                     /gene="coaC"
                     /locus_tag="VYQ30_001459"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1966543.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            6395..6958
                     /locus_tag="VYQ30_001460"
     CDS             6395..6958
                     /locus_tag="VYQ30_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1966544.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         2107 a         1490 c         1393 g         2061 t
ORIGIN      
        1 gcaatgaatc aatcctactt ttatctaaaa atgaaagaac acaaactcaa ggttccttat
       61 acaggtaagg agcgccgtgt acgtattctt cttcctaaag attatgagaa agatacagac
      121 cgttcctatc ctgttgtata ctttcatgac gggcaaaatg tttttaatag caaagagtct
      181 ttcattggac attcatggaa gattatccca gctatcaaac gaaatccgga tatcagtcgc
      241 atgattgtcg ttgctattga caatgatggt atggggcgga tgaatgagta tgcggcttgg
      301 aagttccaag aatctcctat cccagagcag cagtttggtg gtaagggtgt ggagtatgct
      361 gagtttgtca tggaggtggt caagcctttt atcgatgaga cctatcgtac aaaagcagac
      421 tgccagcata cggctatgat tggttcctca ctaggaggca atattaccca gtttatcggt
      481 ttggaatacc aagaccaaat tggttgcttg ggcgtttttt catctgcaaa ctggctccac
      541 caagaagcct ttaaccgcta tatcgagtgc cagaaactat cgcctgacca gcgcatcttc
      601 atctatgtag gaacagaaga agcagatgat acagacaaga ccttgatgga tggcaatatc
      661 aaacaagcct atatcgactc gtcgctttgc tattaccatg atttgatagc agggggagta
      721 catctggata atcttgtgct aaaagttcag tctggtgcca tccatagtga aatcccttgg
      781 tcagaaaatc taccagattg tctgagattt tttgcagaaa aatggtaagt taagaaagga
      841 aaaaacgaaa tgcatattga acatcttagc cactggagtg gtcatcttaa ccgtgaaatg
      901 taccttaacc gttatggaca tggtgggatt ccagttgtgg tctttgcttc atcaggtggt
      961 agtcacaacg aatactatga ttttggcatg attgatgcct gtgcttcctt tatcgaggaa
     1021 ggccttgtcc agttctttac cctatctagt ttggatagtg agagctggtt ggttacttgg
     1081 aaaaatgctc atgaccaagc ggaaatgcac cgtgcctacg aatgttatgt gattgaggag
     1141 gccattcttt tatcaagcac aagacaggtt ggtttgatgg catgatgacg acaggttgct
     1201 ctatgggagc ctatcatgca ctcaatttct tcctccagca tccagatgtc tttaccaaag
     1261 tgattgctct cagtggtgtt tacgacgcac gtttctttgt cggtgattac tacaacgatg
     1321 atgctattta ccaaaactcg ccagtagatt atatttggaa ccaaaacgac ggctggttta
     1381 ttgaccgtta ccgtcaggca gagattgtgc tgtgtacggg gcttggagcc tgggaacaag
     1441 atggtttgcc atccttttac aagctcaaag aagcctttga caagaaacaa attccagcct
     1501 ggtttgctga atggggacat gatgtcgccc atgactggga atggtggcgt aaacaaatgc
     1561 cttatttcct cggtaatctc tatttataaa aggagttacc tatgaattac cttgttattt
     1621 ctccctacta tccacaaaac tttcaacagt ttaccatcga actagctaat aaaggcatca
     1681 cagtcttggg aattggtcaa gagtcttacg agcaattgga tgagcccttg cgcaatagct
     1741 tgaccgagta ttttcgtgtt gataatcttg agaacataga tgaagtcaaa cgtgcagttg
     1801 cttttctctt ttataaacat ggtccaattg accgcatcga gtctcacaat gaatactggc
     1861 ttgagctaga cgcaacactc agagaacaat tcaatgtttt tggtgccaaa ccagaggatc
     1921 tcaaaaagac gaaatataag tctgaaatga agaaactttt caaaaaagca ggtgttcctg
     1981 tggtacctgg agctgttatc aagacggaag cagatgttga tcaagcagtg aaagaaatcg
     2041 gtcttccaat gattgccaaa cctgataatg gagtgggagc agccacaacc tttaaacttg
     2101 agacagaaga cgatatcaat cacttcaagc aagaatggga ccattcaacc ctttatttct
     2161 ttgaaaaatt tgtcacttcc agcgaaatct gtacctttga cgggctcgtg gacaaggatg
     2221 gaaagattgt cttctcaaca acctttgact acgcctatac accgcttgac ctcatgattt
     2281 ataagatgga caattcttat tatgtgctca aggatatgga tcctaaactg cgcaagtatg
     2341 gggaagcaat tgtcaaagaa tttggtatga aagaacggtt tttccatatt gagttcttcc
     2401 gtgaggggga cgattatatt accatcgagt acaataaccg ccctgcaggt ggttttacca
     2461 ttgatgttta taactttgct cattccttgg acctctatcg tggctatgca gctattgtcg
     2521 caggagagga gttcccggcg tcagactttg aaactcagta ttgtttggct acttctcgcc
     2581 gtgcaaatgc tcactatgtt tattcagaag aggatttgct tgccaaatat agccagcagt
     2641 tcaaagttaa aaaagtcatg ccagctgcct tcgcggaact tcaaggagat tacctgtata
     2701 tgctgaccac tccgagtcga caagaaatgg agcagatgat tgcagatttc ggacaacgtc
     2761 aagaataaga actatcggat taaggaaatt aactccctta atccttttgt tttgtctgat
     2821 aaaaaataag agcatcccaa caaggtagct atcataaaac ttgttcgata actatttgaa
     2881 gcaggattag gtggtcagaa attgaatttt aatatttcaa ttgagtcata gtattgtgtt
     2941 tgcgtatcct taaatcagct aaaaggatcc atgacgacac ctatacgata tagttttcaa
     3001 gataccaaac aagtctatta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     3061 gcaggtttct caaaacactg ttttgaggtt gtggatagaa ctgacagagt cagtatcata
     3121 tacctacggc aaggtgaagc tgacgtagtt tgaagagatt ttcgaagagt ataaatctta
     3181 ttgatgaact gcttgcagtc tgagaaaaaa tgagcttgga tattatttcc aaactcactt
     3241 aaagtcaatt tcaatccact agaacaagcc tagtacagtt ccatcgcttt caacatccat
     3301 gttgagagct gctggacgtt ttggaagacc tggcatggtc ataacatcac cagtcaaggc
     3361 aacgatgaag cctgcaccta attttggtac caattcacga atggtaattt caaagttttc
     3421 tggtgctcca agcgcatttg gattgtctga gaaactgtat tgagttttag ccatacagat
     3481 tggcaatttg tcccaaccgt tttgaacgat ttgagcaatt tgtgtttgag atttcttctc
     3541 aaagttcact ttgctaccac gatagatttc agtgacaatt ttttcaatct tttcttggac
     3601 agaaaggtca ttatcgtaca aacgtttata gttagctgga ttttcagcaa ttgtcttaac
     3661 aactgtttcg gcaagtgcta ctccaccttc tgctccatca gcccagacac tagccaattc
     3721 aactggtaca tcgattgagg cacagagttc ttttaagact gcaatttcag cttctgtatc
     3781 agatacaaat tcgttaatag ctacaactgc tggaataccg aacttacgga tattttcaac
     3841 gtggcgtttc aagttagcaa aacctgcacg aactgcctct acattttctt cagtcagagc
     3901 gtctttagcc acaccaccat tcatcttaag ggcacgaagg gttgcgacaa taacaactgc
     3961 atctggagat gttggcaagt ttggtgtctt gatatcaagg aatttctcag caccaaggtc
     4021 cgcaccaaaa ccagcttcag taacagtgta atcagccaag tgaagggctg ttgtcgtcgc
     4081 caaaacagag ttacagccat gagcgatatt ggcaaatgga ccaccgtgta caaaggcagg
     4141 tgtaccgtaa attgtctgaa ccaagtttgg cttaatagca tccttcaaaa tcaaagccaa
     4201 ggcaccctca acctgcaaat cacctacaga aacaggcgta cggtcatagc gataaccaat
     4261 aacgatattc gccaaacgac gtttcaagtc ctcgatgtcc gttgccaagc aaagaattgc
     4321 catgatttct gaagcaactg taatatcaaa accatcctca cgtggaatac cgtttagagg
     4381 accaccaaga ccaacagtca catggcggag cgcacggtcg ttcaagtcca caacgcgttt
     4441 ccagaggata cgacgttgat caattcccag ctcattccct tggtgcaagt ggttgtcaat
     4501 caaggcagaa agggcattgt tggcagttgt aatagcatgc atatctccag taaagtggag
     4561 gttgatgtct tccattggca gaacttgtgc atacccacca ccagcagcac cacccttgat
     4621 ccccatgact ggaccaagag acggttcgcg gatagcaatc atggttttct tgccaatctt
     4681 gttcaaggca tccgcaagac caatggtaag cgtcgacttt ccttcacctg caggtgttgg
     4741 gttgatggca gtaaccaaga tcaatttacc gactggattg ctctcaactg cccgaatttt
     4801 atcaaagctg agtttagcct tgtactttcc gtacaactcc aaatcgtcgt aagaaatacc
     4861 aagtttctct acaacatcaa caattggctt caactcaata ctctgtgcga tttcaatatc
     4921 tgttttcatt caaaattcct ctaacctctt atatgataat tcattatatc acaaaacaag
     4981 atttttaaca tcttaaaact ctctaaacgt tcgtaaatat ctctgttttt aagactttta
     5041 gagtcctttc ttaaatttta tatggcttta tagtttgaaa ctatagtaaa tcttcgtttt
     5101 taccaaaaat ttatcacttt cattttactt accgcttatt tttgtgtaca atagtgctat
     5161 gaaaatttta gttacatcgg gcggtaccag tgaagctatc gatagcgtcc gctctatcac
     5221 taaccattct acaggtcact tggggaaaat tatcacagag actttgcttt ctgcagggca
     5281 tgaagtttgt ttaattacga caaaacgagc tctgaagcca gagcctcatc ctaacctaag
     5341 tattcgagaa attaccaata ccaaggacct tctaatagaa atgcaagaac gtgttcagga
     5401 ttatcaggtc ttgatccact caatggctgt ttctgactac actcctgttt atatgacagg
     5461 gcttgaggaa gttcaggcta gctccaatct aaaagaattt ttaagcaagc agaatcatca
     5521 ggccaagatt tcttcaactg atgaggttca ggttttgttc cttaaaaaga cacccaaaat
     5581 catatcccta gtcaaggaat ggaatcctac tattcatctg attggtttca aactgctggt
     5641 tgatgttacc gaagatcatc tggttgacat tgcacgaaaa agtcttatca agaatcaagc
     5701 agatttaatc atcgcgaatg acctgactca aatttcagca ggtcagcacc gagctatatt
     5761 tgttgagaaa aatcagcttc aaacagtcca gactaaagaa gaaattgcag aactcctcct
     5821 tgaaaaaatt caagcctatc attcttagaa aggaaaacta tggcaaacat tctcttggct
     5881 gtaacgggtt caatcgcctc ttataagtcg gcagatttag tcagttctct aaaaaaacaa
     5941 ggccatcaag tcactgtctt aatgactcag gctgctacag agtttatcca acctttgaca
     6001 ctacaggtac tctcacagaa tcctgtccac ttggatgtca tgaaagaacc ctatcctgat
     6061 caggtcaatc atatcgaact tggaaaaaaa gcagatttat ttatcgtggt acctgcaact
     6121 gctaacacta ttgcaaaact agctcacgga tttgcggaca acatggtaac cagtacagct
     6181 ctagccctac caagtcatat tcccaaacta atagctcctg ctatgaatac aaaaatgtat
     6241 gaccatccag taactcagaa taatctgaaa acattagaaa cctacggcta tcagctgatt
     6301 gctcctaagg aatccctact agcttgtgga gaccacggac gaggagcttt agctgacctc
     6361 acaattattt tagaaagaat aaaggaaact atcgatgaaa aaacgctcta atattgcacc
     6421 cattgctatc ttttttgcta ccatgctcgt gatacacttt ctgagctcac ttatctttaa
     6481 cctttttcca tttccaatca aaccgaccat tgttcatatt cctgtcatta ttgccagcat
     6541 tatttatggt ccacgagttg gggttacact tggatttttg atgggattac ttagcttgac
     6601 ggttaacacg attacgattc taccgacaag ctacctcttc tctcccttcg taccaaacgg
     6661 aaacatctac tcagctatca ttgccattgt cccacgtatt ttgattggtt taactcctta
     6721 cttagtctat aaactgatga aaaacaagac tggtctgatt ttagctgggg ctcttggttc
     6781 cttgacaaat actatctttg tccttggagg aatcttcttc ctatttggaa atgtttataa
     6841 tggaaatatc caacttcttc tggcaaccgt tatctcaaca aattcaattg ctgaattggt
     6901 catttctgca attctaaccc tagccattgt tccacgacta caaaccttga aaaaataaaa
     6961 acaatctccg ctttcaaacc tgaaggtgga gattttgctc tatacagttt ctttttaatg
     7021 aaatctttac tatagtaaaa cgaaataaga a
//
LOCUS       DAVMEF010000098         6961 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMEF010000098 DAVMEF010000000
VERSION     DAVMEF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.098"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            55..1185
                     /locus_tag="VYQ30_001461"
     CDS             55..1185
                     /locus_tag="VYQ30_001461"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1966545.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            1195..2457
                     /locus_tag="VYQ30_001462"
     CDS             1195..2457
                     /locus_tag="VYQ30_001462"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1966546.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLVMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            2461..3258
                     /gene="proC"
                     /locus_tag="VYQ30_001463"
     CDS             2461..3258
                     /gene="proC"
                     /locus_tag="VYQ30_001463"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1966547.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            3478..4116
                     /locus_tag="VYQ30_001464"
     CDS             3478..4116
                     /locus_tag="VYQ30_001464"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1966548.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            4113..5003
                     /locus_tag="VYQ30_001465"
     CDS             4113..5003
                     /locus_tag="VYQ30_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1966549.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            5043..5360
                     /gene="yabA"
                     /locus_tag="VYQ30_001466"
     CDS             5043..5360
                     /gene="yabA"
                     /locus_tag="VYQ30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1966550.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            5363..6232
                     /gene="rsmI"
                     /locus_tag="VYQ30_001467"
     CDS             5363..6232
                     /gene="rsmI"
                     /locus_tag="VYQ30_001467"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1966551.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            6459..>6961
                     /locus_tag="VYQ30_001468"
     CDS             6459..>6961
                     /locus_tag="VYQ30_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1966552.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKW"
BASE COUNT         2071 a         1244 c         1702 g         1944 t
ORIGIN      
        1 tttttcctat caactctttt ctatttcctg ttattcatgt tataatggat aaatatgaat
       61 aatcggagtg agactatgaa atacaaacgg attgtcttta aggtgggtac ttcttctctg
      121 acaaatgagg atggaagttt atcacgtagt aaggtaaagg atattaccca gcagttggct
      181 atgctgcacg aggctggtca tgagttgatt ttggtgtctt caggtgccat tgcggctggt
      241 tttggagcct taggatttaa aaagcgtccg actaaaattg ctgataaaca ggcttcagca
      301 gcggtagggc agggactttt gttggaagaa tatacgacca atcttctctt gcgtcaaatc
      361 gtttctgcac aaatcttgct gacccaagat gactttgtgg ataagcgtcg ttataaaaat
      421 gcccatcagg ctttgtcggt tttgctcaac cgtggggcaa ttcctatcat caatgagaat
      481 gatagtgtcg ttattgatga gctcaaggtt ggggacaatg acactctaag tgctcaagta
      541 gcggcgatgg tccaagcaga ccttttagtt ttcttgacag atgtggacgg tctctatact
      601 ggaaatccta attcagatcc aagagccaaa cgcttggaga gaatcgagac catcaatcgt
      661 gagattattg atatggctgg tggagcaggt tcgtcaaacg gaactggggg tatgttaacc
      721 aaaatcaagg ctgcaactat cgcgacggaa tcaggagttc ctgtttatat ctgctcatcc
      781 ttgaaatcag attccatgat tgaggcggca gaggagaccg aggatggttc ttactttgtt
      841 gctcaagaga aggggcttcg tacccagaaa caatggcttg ccttctatgc tcagagtcaa
      901 ggttctattt gggttgataa aggggctgcg gaagctctct ctcaacatgg aaagagtctt
      961 ctcttatctg gtatcgttga agcagaagga gccttttctt acggtgatat cgtgacagta
     1021 tttgacaagg aaagtggaaa atcacttgga aaaggacgcg tgcaatttgg agcatctgct
     1081 ttggaggata tgttgcgttc tcaaaaagcc aagggtgtct tgatttaccg tgacgactgg
     1141 atttccatta ctcctgaaat ccaactactt tttacagaat tttagaggta aactatggtg
     1201 agtagacaag aacaatttga acaggtacag gctgttaaaa aatcgattaa cacagctagt
     1261 gaagaagtga aaaaccaagc cttgctagtc atggctgatc acttagtggc tgctactgag
     1321 gaaattttag cggctaatgc cctcgatatg gcagcggcta agggaaaaat ctcagatgtg
     1381 atgttggatc gtctttattt ggatgcagat cgtatagaag cgatggcaag aggaattcgt
     1441 gaagtggttg ccttaccaga tccaatcggt gaagttttag aaacaagtca gcttgaaaat
     1501 ggtttggtta tcacaaaaaa acgtgtagct atgggtgtca tcggtattat ctatgaaagc
     1561 cgtccaaatg tgacgtctga tgcggctgct ttgactctta agagtggaaa tgcggttgtt
     1621 cttcgtagtg gtaaggatgc ctatcaaaca acccatgcca ttgtcacagc cttgaagaag
     1681 ggcttggaga cgactactat tcacccaaat gtgattcaac tggtggagga tactagccgt
     1741 gaaagtagtt atgctatgat gaaggccaag ggctatctag accttctcat tcctcgtgga
     1801 ggagctggct tgatcaatgc agtggttgag aatgcgattg tacctgttat cgagacaggg
     1861 actgggattg tccatgtcta tgtggataag gatgcagacg aagacaaggc gctgtctatc
     1921 atcaacaatg ctaaaaccag tcgtccttct gtttgtaatg ccatggaggt tctgctggtt
     1981 catgaaaaca aagcagtaag cttccttcct cgcttggagc aagtgttggt tgcagatcga
     2041 aaagaagctg ggttggaacc aattcaattc cgcctagata gcaaagcaag ccagtttgtt
     2101 tcaggtcaag cagctgagac ccaagacttt gacaccgagt ttttagacta tattctagct
     2161 gttaaggttg tgagcagttt agaagaagcg gttgcgcata ttgaatccca cagtacccat
     2221 cattcggatg ccattgtgac ggaaaatgct gaagctgcag catactttac agatcaagtg
     2281 gactctgcag cggtgtatgt taatgcctca actcgtttca cagatggagg acaatttggt
     2341 cttggttgtg aaatggggat ttctactcag aaattgcacg cgcgtggtcc catgggcttg
     2401 aaggaattga ctagctacaa gtatgtggtc actggtgatg gacagataag ggagtaagag
     2461 atgaagattg gatttatcgg tttggggaat atgggtgcta gtttagccaa gtctgtcttg
     2521 caggctagac cgtcagatga gattctcctt gccaatcgta gtcaagtcaa ggtggatgct
     2581 ttcatcgcta acttcggtgg tcagacttct aacaatgaag aaatattcga agaagcagat
     2641 gtgatttttc taggtgttaa gcctgctcag ttttctgaac tgctttctca ataccagacc
     2701 atccttgaaa aaagagaaag tcttcttttg atttcgatgg cagctggatt gaccttagaa
     2761 aaactagcaa gtcttatccc aagccaacac cgaattattc gtatgatgcc taatacccct
     2821 gcttctatcg ggcaaggagt gattagttat gccttgtctc ctaattgcag ggctgaggac
     2881 agtgagatct tttgtcagct tttagccaag gctggtctct tggttgaatt aggagaaagc
     2941 ttaatcaatg cagcgacagg tcttgcaggt tgtggaccag cctttgtcta tctctttatt
     3001 gaggccttgg cagatgcagg tgttcagacg ggattaccac gagaaacagc cttgaaaatg
     3061 gcagcccaaa ctgtggtagg agctgggcaa ttggtcctag aaagccagca acatcctgga
     3121 gttttgaaag atcaagtttg tagtccaggc ggttcgacta ttgctggcgt agcaagccta
     3181 gaagcgcatg cttttcgagg aacggtcatg gaggcagttc atcaagccta taaacgaaca
     3241 caagaactag gtaaataaga ggtggtttta tctgcctctt ttatggtggt tgatactctt
     3301 cgaaaatcaa attcaaactg cgtcagcttc accttgccgt actcaagtac agcctgcggc
     3361 tagcttccta gtttgctctt tgattttcat tgagtatgaa atgagaagac aaaaagattg
     3421 tcacaaaccc ctattttttt gatagaatag aagtagtaaa aaagaaatga gttagacatg
     3481 tcaaaaggat ttttagtctc tcttgaggga ccagagggag caggcaagac cagtgtttta
     3541 gaggctctgc taccaatttt agaggaaaaa ggagtaaagg tgctgacgac ccgtgaacct
     3601 ggcggagtct tgattgggga gaagattcgg gaagtgattt tggatccaag tcatactcag
     3661 atggatgcta aaacagagct acttctctat attgccagtc gcagacagca tttggtggaa
     3721 aaagttcttc cagcccttga agctggcaag ttggtcatca tggatcgttt tatcgatagt
     3781 tctgttgcct atcagggatt tggtcgtggc ttagatattg aagccattga ctggctcaat
     3841 cagtttgcga cagatggcct caaacccgat ttgacactct attttgacat cgaggtggaa
     3901 gaagggctgg ctcgtattgc tgctaatagt gaccgcgagg ttaatcgttt ggatttggaa
     3961 gggttggact tgcataaaaa agttcgtcaa ggctaccttt ctcttctgga taaagaggga
     4021 aatcgcattg tcaagattga tgctagtctc cctttggagc aagttgtgga aactaccaag
     4081 gctgtcttgt ttgacggaat gggcttggcc aaatgaaaca agatcaacta aaggcttggc
     4141 aaccagctca gtttgaccgt tttgtccgta tcttagaaca agaccagctc aatcacgcct
     4201 atctcttttc aggtttcttt ggaagcttgg aaatggcgca atttttagct aagagcctct
     4261 tttgtacgga taaagttggc gtcttaccat gtgagaaatg ccgaagttgc aagctgattg
     4321 aacaggaaga gtttccagat gtcaccttga ttaagccagt caatcaggtc atcaagacag
     4381 aacgcattcg ggaattggtg ggacagtttt ctcaagcagg gattgaaagc cagcaacagg
     4441 tctttatcat cgagcaagcg gataaaatgc atcccaacgc agccaattct ctgctcaagg
     4501 tcatcgaaga accccagagt gaagtttata ttttcttctt gactagcgat gaggaaaaga
     4561 tgttaccgac aatccgaagt cggactcaga tcttccactt taaaaagcaa gaagaaaaac
     4621 ttatcttact cttagaacaa atgggacttg ttaagaaaaa agcgactctt ttagctaagt
     4681 ttagtcaatc gcgagctgaa gcagaaaagt tggctaatca ggcaagtttt tggaccttgg
     4741 tcgatgaaag tgaacgcctg ctgacttggt tagtagctaa gaaaaaagaa agttatctac
     4801 aggttgccaa attagccaac ttggcagatg ataaggaaaa acaggatcag gttttacgga
     4861 ttcttgaagt tctctgtggg caggacctct tgcaggtaag agtaagagtg attctacaag
     4921 atttactaga agctagaaaa atgtggcaag ctaatgtcag ctttcaaaat gccatggaat
     4981 atctggtctt gaaagaaata taaactcaaa aatgaatgat aaagaaagga aagggctgtt
     5041 ttatggacaa aaaagaatta tttgacgcgc tggatgattt ttcccaacaa ttattggtaa
     5101 ctttagccga tgtggaagcc atcaagaaaa atctcaagag cttggtagaa gaaaatacag
     5161 ctcttcgctt ggaaaatagt aagttgcgag aacgcttggg tgaggtggaa gcagacgctc
     5221 ccgtcaaggc caagcatgtt cgcgaaagtg tccgtcgcat ttaccgtgat ggatttcacg
     5281 tatgtaatga tttttatgga caacgtcgag agcaggacga agaatgtatg ttctgtgatg
     5341 agttgttata cagggagtaa gcatgcaaat tcaaaaaagt tttaaggggc agtctcccta
     5401 tggcaagctg tatctagtgg caacgccgat tggcaatcta gatgatatga cttttcgtgc
     5461 tatccagacc ttgaaagaag tggactggat tgctgctgag gatacgcgca atacagggct
     5521 tttgctcaag cattttgaca tttccaccaa gcagatcagt tttcatgagc acaatgccaa
     5581 ggaaaaaatt cctgatttga ttggtttctt gaaagcaggg caaagtattg ctcaggtctc
     5641 tgatgccggt ttgcctagca tttcagaccc tggtcatgat ttagttaagg cagctattga
     5701 ggaagaaatt gcagttgtga ctgttccagg tacctctgca ggaatttctg ccttgattgc
     5761 cagtggttta gcgccacagc cacatatctt ttacggtttt ttaccgagaa aatcaggtca
     5821 acagaagcaa ttttttggct ctaaaaaaga ttatcctgaa acacagattt tttatgaatc
     5881 acctcatcgt gtagcagaca cgttggaaaa tatgttagaa gtctacggtg accgctcggt
     5941 tgttttggtc agggaattga ccaaaatcta tgaagaatac caacgaggta caatttctga
     6001 attactggaa agcatcgctg aaacgccact caagggcgaa tgccttctta ttgttgaagg
     6061 tgccagtcag gatgtggagg aaaaagacga ggaggacttg ttcttagaaa tccaagcccg
     6121 tatccagcaa ggcatgaaga aaaatcaagc tattaaggaa gtagctaaga tttaccagtg
     6181 gaataagagt caactctacg ctgcctacca cgattgggaa gaaaaacaat aaagggagac
     6241 aggatgtaat aattctgtct gtttctgttt aacttaatta gtgatgataa tataaagatg
     6301 tatcacttgg tatagaagct ttggtattaa gttttttatt aagcccatac ggaataccga
     6361 tgtttggagc agcagttata gcgttcttag aaggtataaa tagaaaaata aggtcatttt
     6421 aaatcaaagg attgataaat cagaaagaag gtgatttttt gcgaacatac gaaaataaag
     6481 aagaactaaa agctgagata gagaaaacat ttgagaaata tattttagaa tttgataata
     6541 ttccagaaaa tttaaaagat aagagagctg atgaagttga cagaactcca gcagaaaacc
     6601 ttgcttatca ggttggttgg accaacttgg ttcttaaatg ggaagaagat gaaagaaagg
     6661 ggcttcaagt aaaaacacca tcggataaat ttaaatggaa tcaacttggt gaattgtatc
     6721 agtggttcac agatacctac gctcatttat ctctgcaaga gttgaaagca aaattaaatg
     6781 aaaatattca ttctatctct gcaatgattg attcgttgag tgaggaagaa ttatttgaac
     6841 cgcatatgag aaagtgggct gatgaagcga ctaaaacagc gacttgggaa gtgtataagt
     6901 ttattcatgt aaatacggtt gcaccttttg gaactttcag aactaaaatc agaaaatgga
     6961 a
//
LOCUS       DAVMEF010000099         6943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVMEF010000099 DAVMEF010000000
VERSION     DAVMEF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.099"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VYQ30_001469"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VYQ30_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1966553.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VYQ30_001470"
     CDS             2483..4144
                     /locus_tag="VYQ30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1966554.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHCYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VYQ30_001471"
     CDS             4213..4992
                     /locus_tag="VYQ30_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1966555.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VYQ30_001472"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VYQ30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1966556.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..>6943
                     /gene="murN"
                     /locus_tag="VYQ30_001473"
     CDS             6329..>6943
                     /gene="murN"
                     /locus_tag="VYQ30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1966557.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKET"
BASE COUNT         2100 a         1153 c         1677 g         2013 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 agctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actacgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag ggatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatacct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccctatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaaga cctattatct
     1981 gacaggttat gggactagag atcagtttgc taaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtattagaa cttttagaat aagagggtag agttgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccattgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtaga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcacacgcgt ggatttgttt atctcaagaa
     3961 gagtcgcgat attctacgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt ataccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggctacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattaa agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagcgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ttggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatt caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acc
//
LOCUS       DAVMEF010000100         6926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVMEF010000100 DAVMEF010000000
VERSION     DAVMEF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.100"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..137)
                     /locus_tag="VYQ30_001474"
     CDS             complement(<1..137)
                     /locus_tag="VYQ30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1966558.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            342..530
                     /locus_tag="VYQ30_001475"
     CDS             342..530
                     /locus_tag="VYQ30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1966559.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            687..1052
                     /locus_tag="VYQ30_001476"
     CDS             687..1052
                     /locus_tag="VYQ30_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1966560.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            1055..2722
                     /locus_tag="VYQ30_001477"
     CDS             1055..2722
                     /locus_tag="VYQ30_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1966561.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            2923..4623
                     /locus_tag="VYQ30_001478"
     CDS             2923..4623
                     /locus_tag="VYQ30_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1966562.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            4616..5092
                     /gene="ilvN"
                     /locus_tag="VYQ30_001479"
     CDS             4616..5092
                     /gene="ilvN"
                     /locus_tag="VYQ30_001479"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1966563.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            5158..6180
                     /gene="ilvC"
                     /locus_tag="VYQ30_001480"
     CDS             5158..6180
                     /gene="ilvC"
                     /locus_tag="VYQ30_001480"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1966564.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            6287..6523
                     /locus_tag="VYQ30_001481"
     CDS             6287..6523
                     /locus_tag="VYQ30_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966565.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            6761..>6926
                     /locus_tag="VYQ30_001482"
     CDS             6761..>6926
                     /locus_tag="VYQ30_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1966566.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTL"
BASE COUNT         2190 a         1213 c         1577 g         1946 t
ORIGIN      
        1 tttcctttct gttgataggc tagtagcaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta tcaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aatcacaaca caaataaaaa
      241 atcttccaat tcccttgcaa aaaacaagaa agtgtgataa catagtacgg tatgtggtgc
      301 tagcacatcc gctatattag atctaatagg aggaaaacac aatggctaaa gtatgttact
      361 ttacaggtcg taagactgta tcaggaaaca accgttcaca cgcgatgaac caaacaaaac
      421 gtgccgtaaa accaaacctt caaaaagtta ctgttcttat cgatggtaaa cctaaaaaag
      481 tttgggcttc agctcgtgct ttgaaatcag gtaaagttga acgcgtttaa taataaaagc
      541 aaaggagacc ttagggtctt ttttctttta ttgtgggtat aacatcgttt gaaaaataga
      601 gtaaatatcc gctgatacat cattctgctt ttacacttgg gctgaaatat gataaaatag
      661 agtatcaact agttgaggta aaaaggatga ctgtaaaaat taatacaaaa gatggtcaaa
      721 tcgaactaac agatgaagtg attgcaaccg tcgtaggtgg agcagcaact gagatttttg
      781 gtgtggtcgg tatggctagt aaaaatgccc tcaaagataa tttccaagcc ctgctaggta
      841 aggaaaatta ttctaaaggt gtcgtcgtaa aggcagccga agatggcagt attgcagttg
      901 atgtatatac cgtgttaagc tacggaacaa agattagtga agtgtcaaaa aacattcaag
      961 agcgtgttgc ctttagcttg gaaaaccagc ttggaattac tgctcagact gtaaatgtct
     1021 acattcaaaa tatcaaagtt gtaggagaat aaccgtgtca aaaattacta ctagcttatt
     1081 tcaagaaatg gtgcaggctg catcaactcg cttgaataag caagctgaat atgtcaattc
     1141 attaaacgtc tttccagttc cagatggaga tactgggaca aatatgggaa tgaccattga
     1201 aaatggtgct aaagaagttg cagacaagcc agcttctaca gttggagaag tagcgagcat
     1261 tcttgctaaa gggcttttga tgggtgcgcg tgggaactca ggagtgatta cgtctcagct
     1321 tttccgtgga ttttcccaag ctatcaagga taaagacgag ttaacaggtc aagacttggc
     1381 tctggccttc caatcaggtg tggaagttgc ctataaggca gtgatgaaac ctgttgaagg
     1441 aacgattttg acagtttctc gtggagctgc tatcggtgct aagaaaaaag ctgagcaaac
     1501 agatgatact gttgaagtca tgcgcgcagc cttggaaggt gctaaaacag ctctagctaa
     1561 aacaccagac atgcttccag tattgaaaga agttggcgtt gtggactcag gtggtcaagg
     1621 attggtcttc atctacgaag gtttcctttc agcccttact ggcgaatata ttgcatctga
     1681 ggactttgta gcgactcctg ccaacatgag tgagatgatc aatgtagagc atcataagtc
     1741 tgtagctggt cacgtagcga ctgaggacat cacgtttggt tactgtactg aaatcatggt
     1801 agctcttaag caaggtccaa cctatgctaa agattttgac tacgatgaat tccgtaacta
     1861 cttggatgag cttggggatt ctctcctcgt tgttaacgat gatgaaattg tcaaagtcca
     1921 tgttcataca gaagatccag gacttgttat gcaagaaggt ctcaaatatg gtagcttggt
     1981 caaggtaaaa gttgacaata tgcgtaacca acacgaagca caggttgaga aagaagctac
     2041 tcaagttatc aagtcggctg aagaaaaaga gtatgctttg attgctgtgg tggctggtaa
     2101 aggtctagca gatatcttct gttctcaagg cgtggattat gttatcgaag gcggtcaaac
     2161 catgaaccct tcaacagaag actttatcaa ggctgttgaa caggtcaatg cccgtaacat
     2221 catcttcttg ccaaacaaca agaacatctt catggcagct caatctgcgg cagaagtttt
     2281 ggagcaacca gcagtagtgg tagaggctcg cactcttcct caaggtatga caagtcttct
     2341 tgcctttgat ccaagcaagt ccattgaaga aaaccaagag cgtatgacag ctgctcttag
     2401 agatgtcgtt agcggaagcg tcacaacagc cgtgcgtgat acaacgatcg atggcttaga
     2461 aatccatgaa aacgataatc taggtatggt ggatggaaaa attcttgtgt caaaccctga
     2521 tatgcaccaa acattgactg aaaccttgaa acatatgttg gatgaagaca gtgaaatcgt
     2581 aaccttctat gtcggtgaag acggaagcga agaacttgcc aatgaaatcg ctcaagaaat
     2641 cgtagaagaa ttcgaagacg ttgaagtcga gattcaccaa ggtcaacaac ctgtttaccc
     2701 atacctattt agtgtggaat aagattaatg aaggactgag aaatcagttc ttttttcttt
     2761 tttagtaaat gaaatcggta tctttttaat aaaaacaaaa ataacattca taaataaacg
     2821 ttaaaataga aaattcagaa aatttcttct tttatcttga aaaattttga aaaaatggta
     2881 tgatagtaac aagttatttt taagaggaaa gaaaggggaa caatggagaa aatcagttta
     2941 gaatctccta agacggggtc ggacctagtt ttggaaacac ttcgtgattt aggagttgat
     3001 accatctttg gttatcctgg tggtgcggtt ttgccttttt atgatgcgat atataatttt
     3061 aaaggcattc gccacattct agggcgccat gagcaaggtt gtttgcatga agctgaaggt
     3121 tatgccaaat caactggaaa gttgggtgtt gccgtcgtca ctagtggacc aggagcaaca
     3181 aatgccatta cagggattgc ggatgctatg agcgatagcg ttcccctttt ggtctttaca
     3241 ggtcaggtgg cgcgagcagg gattgggaag gatgcctttc aggaggcaga catcgtagga
     3301 attaccatgc caatcactaa gtacaattac caagttcgtg agacagctga tattccgcgt
     3361 atcattacgg aagctgtcca tatcgcaact acaggccgtc cagggccagt tgtaattgac
     3421 ctaccaaaag acatatctgc tttagaaaca gacttcattt attcaccaga agtgaattta
     3481 ccaagttatc agccgactct tgagccgaat gatatgcaaa tcaagaaaat cttgaagcaa
     3541 ttgtccaagg ctaaaaagcc agtcttgtta gctggtggtg gaattagtta tgctgaggct
     3601 gctacggaac taaatgaatt tgcagaacgc tatcaaattc cagtggtaac cagtcttttg
     3661 ggacaaggaa cgattgcaac gagtcaccca ctctttcttg gaatgggagg catgcacggg
     3721 tcattcgcag caaatattgc catgacggaa gcggacttta tgattagtat tggttctcgt
     3781 ttcgatgacc gtttgacggg gaatcctaag actttcgcta agaatgctaa ggttgcccac
     3841 attgatattg acccagctga gattggcaag attatcagtg cagacattcc tgtagttgga
     3901 gatgctaaga aggccttgca aatgttgcta gcagaaccaa cagttcacaa caatactgaa
     3961 aagtggattg agaaagtcac taaagacaag aatcgtgttc gttcttatga caagaaagag
     4021 cgtgtggttc aaccgcaagc agttattgaa cgaattggtg aattgacgaa tggagatgcc
     4081 attgtggtaa cagacgttgg tcaacaccaa atgtggacag ctcagtatta tccctaccaa
     4141 aatgaacgtc agttagtgac ttcaggtggt ttgggaacaa tgggctttgg aattccagca
     4201 gcaatcggtg ctaaaattgc taacccagat aaggaagtag tcttgtttgt tggggatggt
     4261 ggtttccaaa tgactaacca ggagttggct attttgaata tttacaaggt gccaatcaag
     4321 gtggttatgc tgaacaatca ttcactcgga atggttcgcc agtggcagga atccttctat
     4381 gaaggcagaa catcagagtc ggtctttgat acccttcctg atttccaatt gatggcgcag
     4441 gcttatggca ttaaaaacta taagtttgac aatcctgaga cattggctca agaccttgaa
     4501 gtcatcactg aggatgttcc tatgctaatt gaggtagata tttctcgtaa ggaacaggtg
     4561 ttaccaatgg taccggctgg taagagtaat catgagatgt tgggggtgca gttccatgcg
     4621 tagaatgtta acagcaaaac tacaaaatcg ttcaggagtc ctcaatcgct ttacaggtgt
     4681 cctatctcgt cgtcaggtta atattgaaag catctctgtt ggagcaacag aagatccgaa
     4741 tgtatcgcgt atcactatta ttattgatgt tgcttctcat gatgaagtgg agcaaatcat
     4801 caaacagctc aatcgtcaga ttgatgtgat tcgcattcga gatattacag acaagcctca
     4861 tttggagcgc gaggtgattt tggttaagat gtcagcgcca gctgagaaga gagctgagat
     4921 tttagcgatt attcaacctt tccgtgcaac agtggtagac gtagcgccaa gctcgattac
     4981 cattcagatg acgggaaatg cagaaaagag cgaagcccta ttgagagtca ttcgcccata
     5041 cggtattcgc aatattgctc gaacgggtgc aactggattt acccgcgatt aaaaatccaa
     5101 cttaaattta ttaaaccagc ctaaaaggca ataaataata gaaaagagag aaaagctatg
     5161 acagttcaaa tggaatatga aaaagatgtt aaagtagcag cacttgacgg taaaaaaatc
     5221 gccgttatcg gttatggttc acaagggcat gcgcatgctc aaaacttgcg tgattcaggt
     5281 cgtgacgtta ttataggtgt acgtccaggt aaatcttttg ataaagcaaa agaagatgga
     5341 tttgatactt acacagtagc agaagctact aagttggctg atgttatcat gatcttggcg
     5401 ccagacgaaa ttcaacaaga attgtacgaa gcagaaatcg ctccaaactt ggaagctgga
     5461 aacgcagttg gatttgccca tggtttcaac atccactttg aatttatcaa agttcctgcg
     5521 gatgtagatg tcttcatgtg tgctcctaaa ggaccaggac acttggtacg tcgtacttac
     5581 gaagaaggat ttggtgttcc agctctttat gcagtatacc aagatgcaac aggaaatgct
     5641 aaaaacattg ctatggactg gtgtaaaggt gttggagcgg ctcgtgtagg tcttcttgaa
     5701 acaacttaca aagaagaaac tgaagaagat ttgtttggtg aacaagctgt actttgtggt
     5761 ggtttgactg cccttatcga agcaggtttc gaagtcttga cagaagcagg ttacgctcca
     5821 gaattggctt actttgaagt tcttcacgaa atgaaattga tcgttgactt gatctacgaa
     5881 ggtggattca agaaaatgcg tcaatctatt tcaaacactg ctgaatacgg tgactatgta
     5941 tcaggtccac gtgtaatcac tgaacaagtt aaagaaaata tgaaggctgt cttggcagac
     6001 atccaaaatg gtaaatttgc aaatgacttt gtaaatgact ataaagctgg acgtccaaaa
     6061 ttgactgctt accgtgaaca agcagctaac cttgaaattg aaaaagttgg tgcagaattg
     6121 cgtaaagcaa tgccattcgt tggtaaaaac gacgatgatg cattcaaaat ctataactaa
     6181 ttagaaatat atagcgctgg agatgatttt atgaaaaaga ttatgagaaa aattgcatcg
     6241 ttattattgg ttctagttgt ataatgtaat tacaccgtcg gtaatagtgc tagcagacca
     6301 aaataaagca gattggtcgt atgatgaaaa tgctgtaatt aacatttatg atgatgctaa
     6361 ttttgaagat ggtaggttgc atatgaactt tgaacaattc ttcaaattgg cacaaatatc
     6421 tagagaagaa ggtcttgaaa ttcattctcc gtttgagaga gctggtgcga ctaaatctgc
     6481 tcgttatata gcgaaatgga ttttgagaaa taaaaaacat taacaaatat agttggtaaa
     6541 tcattaggac ctaaatcagc tgttagattc ggagaagctt tatcctatat tgaaggtcct
     6601 cttcgcagaa taaatgagac gatagatggc ggtttatatc aaatagagca aattattgca
     6661 tctggattga aagaatcggg tttaaatgac tggactgcga aaactttagc ttcagctatt
     6721 cgtgggatat tagatgtact tatttagggg ttgaaatcat atgaatatta ccaatttgtt
     6781 ttctatcaag acaggatgtg atgaaactga taggcaactg caaaaactat tttttcagtt
     6841 ggatttacaa ttgggagaat tgacagatca actaagaaaa ttagattcta attttgttcc
     6901 tcgtagtcaa tttgtagaca cgttgg
//
LOCUS       DAVMEF010000101         6891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMEF010000101 DAVMEF010000000
VERSION     DAVMEF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.101"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            71..1348
                     /locus_tag="VYQ30_001483"
     CDS             71..1348
                     /locus_tag="VYQ30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966567.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1361..2008
                     /gene="vex2"
                     /locus_tag="VYQ30_001484"
     CDS             1361..2008
                     /gene="vex2"
                     /locus_tag="VYQ30_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1966568.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDICKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2060..3439
                     /gene="vex3"
                     /locus_tag="VYQ30_001485"
     CDS             2060..3439
                     /gene="vex3"
                     /locus_tag="VYQ30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1966569.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3620..3703
                     /gene="pep27"
                     /locus_tag="VYQ30_001486"
     CDS             3620..3703
                     /gene="pep27"
                     /locus_tag="VYQ30_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1966570.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            3752..4408
                     /gene="vncR"
                     /locus_tag="VYQ30_001487"
     CDS             3752..4408
                     /gene="vncR"
                     /locus_tag="VYQ30_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1966571.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4405..5733
                     /gene="vncS"
                     /locus_tag="VYQ30_001488"
     CDS             4405..5733
                     /gene="vncS"
                     /locus_tag="VYQ30_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1966572.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKNRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLIKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            5874..6755
                     /locus_tag="VYQ30_001489"
     CDS             5874..6755
                     /locus_tag="VYQ30_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1966573.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
BASE COUNT         2113 a         1349 c         1513 g         1916 t
ORIGIN      
        1 ttttttttcg tatttcataa acatttcata tttgggtttt ataatagtct tacaaatatg
       61 gaggtgacaa atgaatccaa tccaaagatc ttgggcttat gtcagcagaa agcgactgag
      121 aagttttatt ttatttctga ttttattggt cttattggcc ggaatttcag cctgtttgac
      181 tctgatgaag tccaacaaaa cagtagaaag caatctgtat aaatcactca atacatcttt
      241 ttctattaag aagatagaga atggtcagac attcaagttg tcagacctag catctgtaag
      301 caagattaag gggctggaaa atgtctctcc tgaacttgag acggtcgcaa aactaaaaga
      361 caaggaagca gtgactggcg agcagagcgt ggagcgtgat gatttatcag ctgcagacaa
      421 taacttggtt agcttaacgg ctcttgagga ttcatccaag gatgtaacct ttaccagttc
      481 ggctttcaat ctaaaagaag ggcgacacct tcaaaaaggg gattccaaga aaatccttat
      541 ccacgaagaa ttggctaaga agaacggtct ttcacttcat gataagattg gcttggatgc
      601 tggtcagtct gaatctggaa aaggacaaac agtagagttt gagattatcg gcatcttttc
      661 tggtaaaaaa caagagaaat tcacaggctt gtcttctgac ttcagtgaaa atcaagtctt
      721 tacagactat gaaagtagcc aaaccctttt gggcaatagt gaagctcaag tcagtgcagc
      781 acgcttctat gtagaaaatc ctaaggaaat ggacggactc atgaagcagg tagaaaactt
      841 ggccttggaa aatcaaggct accaagtcga aaaggaaaac aaggcttttg aacaaatcaa
      901 agactcagtt gcaactttcc aaaccttcct gaccatcttc ctttatggga tgttgatagc
      961 aggagctgga gccttaattc tggttttgtc tctctggttg agagaacggg tctatgaagt
     1021 ggggatttta cttgcacttg gaaaaggcaa gagctcgatc ttcctacaat tctgtttaga
     1081 ggtagttttg gtatctcttg gagctttgct tccagcattt gttgcaggaa acggaatcac
     1141 aacttaccta ctccaaactc tactagcaag tggagatcag gcaagcttac aagatacact
     1201 agccaaagca agcagtttat caactagcat cttatctttt gcagaatcct atgtttttct
     1261 agttctgctt agttgcttat ctgtagccct ttgtttccta ttcttattta gaaaatcacc
     1321 gaaagaaatt ttatcatcta ttagttaaga aggagaaatc atgactttat tacaattaca
     1381 agatgttacc taccgttata agaatactgc tgaagcagtc ctatatcaga tcgattataa
     1441 ttttgaacct ggaaaatttt acagtattat tggggagtca ggagcaggaa aatccacact
     1501 cttgtcccta cttgctggtc tagatagtcc tgttgaaggt tctatccttt ttcaaggaga
     1561 ggatatttgt aagaagggct attcttacca tcgcatgcac catatttccc tggtctttca
     1621 aaattataac ttgatagatt atctttctcc gctggaaaat atccgcttgg tcaacaaaaa
     1681 ggcaagcaag gatacacttc ttgagcttgg tttggatgaa agccagatca agcggaatgt
     1741 tctccagtta tcaggtggtc aacagcaacg tgttgccatt gctcgcagtt tggtctcaga
     1801 agctccagtt attctagctg atgagccaac aggaaatctg gatcctaaaa ctgctggaga
     1861 tattgtcgaa ctactcaaat cacttgccca gaaaacaggt aaatgtgtga ttgtcgtaac
     1921 tcacagtaaa gaagtggcac aagcgtcaga tattacactt gaattaaagg ataagaaact
     1981 gactgaaacg cgcaatacta gtaaataatt tgagcttact ttaatagaat gattaaaaca
     2041 aaatctagaa agggaatcta tgttacacaa cgcatttgcc tatgttacaa ggaagttttt
     2101 caaatcgatt gtcatcttcc tgattattct cctcatggcg agcttgagtt tggtcggctt
     2161 gtcaatcaag ggagctactg ccaaggcttc tcaggagacc tttaaaaata tcaccaatag
     2221 cttctccatg caaatcaatc gtcgcgtcaa ccaaggaacg cctcgcggtg ctgggaatat
     2281 caagggtgaa gacatcaaaa aaatcaccga aaacaaggcc attgagtctt atgtcaaacg
     2341 tatcaacgct atcggagatt tgactggata tgacctgatt gaaacgccag aaaccaagaa
     2401 gaatctcact gctgatcgtg ccaaacgttt tggaagtagc ttgatgatta caggtgtcaa
     2461 tgactcctct aaagaagaca agtttgtctc tggttcttat aaactagtcg aaggagagca
     2521 cttaaccaac gacgacaagg ataaaatcct cttgcacaag gacttggcag ccaaacacgg
     2581 ctggaaagta ggggataagg ttaaactgga ctctaatatc tacgatgcag ataatgaaaa
     2641 aggagccaag gaaacagttg aagtgacaat caagggactc tttgatggtc ataataagtc
     2701 agcagtaacc tactcacaag aactttacga aaacacagct attacagaca ttcacactgc
     2761 tgcaaaactt tatggataca cagaagacac agccatttat ggggacgcaa ccttctttgt
     2821 aacagcagac aagaacttgg atgatgttat gaaagagttg aatggcatca gtggtatcaa
     2881 ctggaagagc tacacactcg tcaagagctc ctctaactac ccagctcttg agcaatctat
     2941 ctctggtatg tacaagatgg ccaacctcct cttctggggt agcttgagct tctcagttct
     3001 cctccttgcc ctcttgctca gcctttggat caacgcccgt cgcaaggaag tgggaattct
     3061 cctctctatc ggcctcaagc aggcaagtat cttgggtcaa ttcatcaccg aatctatctt
     3121 gattgctatc cctgctctag tttctgctta cttcctagct aattacactg cccgtgcaat
     3181 tggaaacact gtccttgcca atgtgacttc aggtgttgcc aaacaggcta gtaaggcggc
     3241 tcaagcctct aaccttggtg gtggtgcaga agtagatggc tttagcaaga ccttgtcgag
     3301 cctagacatt tccattcaga catcagactt tatcatcatt tttgtccttg ccttggttct
     3361 agtggttctc gttatggcgc ttgcttcaag caatctcctt agaaaacaac caaaagagct
     3421 cttgctggat ggtgaataaa tttgaaaaaa atgagtctgg aataaagatt gcatcttgtg
     3481 tttctattca agaatagtgg ataggaatag ctatttaaca attcaaaata aatctgaaag
     3541 cagtggtgaa gatcattgct ttcagttgct ttctttgtac tttagtgctt aaatataata
     3601 tactaaagtt atggaattta tgagaaagga atttcacaac gttttatcta gtggtcagtt
     3661 gcttacagac aaaaggccag caagagacta taatagaaaa tagagtaggt atttattcta
     3721 agaaaaataa aaaatagaga gcagttaaag tatgaaaatt ttaattgtag aagatgaaga
     3781 gatgatccgt gagggggtca gtgattattt gacggattgt ggctatgaaa ctattgaggc
     3841 agcggacggt caggaagctc tggagcaatt ttctagctat gaggtggccc tggttttact
     3901 ggatatccag atgcccaagc ttaacggctt agaagtccta gctgagattc gtaaaaccag
     3961 tcaggttcct gtcttgatgt tgacagcttt tcaggatgag gaatacaaga tgagtgcctt
     4021 tgcctctttg gcagatggct atctggaaaa acctttctcc ctctccctct taaaagtgag
     4081 ggtggacgcg attttcaagc gctactacga tacaggacga atcttttctt acaaggatac
     4141 caaggtggac tttgaaagct acagtgcaag cctcgcaggt caagaagtgc ctatcaatgc
     4201 caaagagttg gaaattctgg actatctagt gaaaaatgaa ggccgggcct tgactcggtc
     4261 tcagattatc gatgccgtct ggaaagcgac agatgaggtt ccctttgacc gtgttattga
     4321 tgtttatatc aaggaattgc ggaaaaagct agacttggat tgtatcctca ctgtgcgcaa
     4381 tgttggttat aaattggagc gaaaatgaaa cgaacaggtt tatttacaaa gatatttatc
     4441 tataccttct cgatatttag tgttctggtt atctgccttc atttagctat ttattttctt
     4501 tttccttcga cttatctgag tcatcgtcag gaaaccattg gtcaaaaggc aacagccatt
     4561 gcccagtccc tagaagggaa agataggcag agtatcgagc aagtgttaga cttgtattcc
     4621 cagactagtg atatcaaggg gaccgtcaaa ggtgagatga ccgaggacaa gttagaagtc
     4681 aaggacagtc ttcctctgga cacagaccgc cagacaacct ctctctttat tgaggagcgc
     4741 gaggtgaaaa cgcaagacgg tggtactatg attctccagt ttctagcttc catggattta
     4801 caaaaggaag cggagcaaat cagtctccag tttcttccct ataccttgct ggcctccttt
     4861 ctgatttccc tcttggtggc ctacatctac gctcggacta ttgttgcacc gattttggaa
     4921 atcaagcggg tgacccgtcg gatgatggac ctggattccc aagtgcgatt gcgcgtggat
     4981 tctaaggatg agataggcaa tctcaaggaa caaatcaata gcctctacca gcatctcttg
     5041 actgttattg cggacttgca tgaaaagaat gaagccattc tccagctgga gaagatgaag
     5101 gtcgaattcc tacgaggagc ttctcatgaa ttgaaaacac cgctggctag tttgaaaatc
     5161 ctaatcgaaa atatgagaga gaatatcggt cgttataaga atagagacca gtatctggga
     5221 gttgccttgg ggattgtgga tgaactcaat caccatgttc tgcagatact ttccctctct
     5281 tctgtgcagg aattgcgaga tgatagggaa acaattgacc tcctccagat gacgcaaaat
     5341 ctgatcaaag attatgcctt gctagccaag gaaagagagc tccagataga caatagtttg
     5401 acccatcagc aggcttatct aaacccatca gttatgaagt tgattctttc taatctcatc
     5461 agcaatgcca ttaagcactc tgttccaggt ggcttagttc gaattggaga aagagaagga
     5521 gaacttttta tcgaaaatag ctgtagctca gaggaacaag aaaaactagc ccagtctttt
     5581 tctgacaatg ctagtcgcaa ggtcaagggg tctggtatgg ggctctttgt ggttaagagt
     5641 ctattagaac atgaaaaatt agcttatcgt ttcgagatgg aggagaatcg tttaaccttc
     5701 tttatagctt ttccaaaagt cgcccaagac tagggagaga aagggtttac atagatggag
     5761 ttagaagaaa atcaatcgaa actgcgggaa aaactagatt tttttggcaa aaagtgataa
     5821 aatgaacaat gtaaatggga tgacccataa aaatatacag gaggcctgat aaaatggcaa
     5881 tcgtttcagc agaaaaattt gtccaagcag cccgtgacaa cggttatgca gttggtggat
     5941 ttaacacaaa caaccttgag tggactcaag ctatcttgcg cgcagcagaa gctaaaaaag
     6001 ctccagtttt gatccaaact tcaatgggtg ctgctaaata catgggtggt tacaaagttg
     6061 ctcgcaactt gatcgctaac cttgttgaat caatgggtat cactgtacca gtagctatcc
     6121 accttgacca cggtcactac gacgatgcac ttgagtgtat cgaagttggt tatacttcaa
     6181 tcatgtttga cggttcacac cttccagttg aagaaaacct taaattggct aaagaagttg
     6241 ttgaaaaagc acacgctaaa ggtatctcag tagaagctga agttggtact atcggtggtg
     6301 aagaagacgg aatcatcggt aaaggtgaat tggctccaat cgaagacgct aaagcaatgg
     6361 ttgaaactgg tattgacttc ttggcagctg gtatcggtaa catccacggc ccttacccag
     6421 taaactggga aggtcttgac cttgaccact tgcaaaaatt gacagaagct cttccaggat
     6481 tcccaatcgt attgcacggt ggatcaggta ttcctgatga gcaaatccaa gcagctatca
     6541 aacttggtgt tgccaaagtt aacgttaaca cagaatgcca aatcgcattc gctaacgcaa
     6601 ctcgtaaatt tgctcgtgac tacgaagcaa acgaagcaga atacgacaag aaaaaactct
     6661 tcgacccacg taaattcttg gctgacggtg taaaagctat ccaagcatcg gttgaagaac
     6721 gtatcgacgt attcggttca gaaggtaaag cataatctag ctgaataata cagataaaac
     6781 ctgcctgtga gggtgggttt tttgatttca agctactcgt tttagtatag taaactgaat
     6841 ctagactagc acaatgctgc ttctatagcg ttttgaaact agaataggac a
//
LOCUS       DAVMEF010000102         6873 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMEF010000102 DAVMEF010000000
VERSION     DAVMEF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.102"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..379
                     /locus_tag="VYQ30_001490"
     CDS             <1..379
                     /locus_tag="VYQ30_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966574.1"
                     /translation="RGKVSGRRYQKISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEALLSCSCFN"
     gene            569..1408
                     /locus_tag="VYQ30_001491"
     CDS             569..1408
                     /locus_tag="VYQ30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966575.1"
                     /translation="MLAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVS
                     ASIVESAAETSKIAQFQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYF
                     ILELKDPSQTKVVTEELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAA
                     VMIGIIIYINIMQRSKEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVG
                     SLANAIVSHFYPSITKVFELNLLSVLGTLVFALLLGYVSAYFYLVMSQPTSRRVRLVK
                     WIL"
     gene            1605..2612
                     /gene="gap"
                     /locus_tag="VYQ30_001492"
     CDS             1605..2612
                     /gene="gap"
                     /locus_tag="VYQ30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1966576.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2680..3516)
                     /locus_tag="VYQ30_001493"
     CDS             complement(2680..3516)
                     /locus_tag="VYQ30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1966577.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEISLLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3642..5837
                     /locus_tag="VYQ30_001494"
     CDS             3642..5837
                     /locus_tag="VYQ30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1966578.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5890..6042
                     /gene="rpmG"
                     /locus_tag="VYQ30_001495"
     CDS             5890..6042
                     /gene="rpmG"
                     /locus_tag="VYQ30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1966579.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6052..6228
                     /gene="secE"
                     /locus_tag="VYQ30_001496"
     CDS             6052..6228
                     /gene="secE"
                     /locus_tag="VYQ30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1966580.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6283..6819
                     /gene="nusG"
                     /locus_tag="VYQ30_001497"
     CDS             6283..6819
                     /gene="nusG"
                     /locus_tag="VYQ30_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1966581.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2129 a         1209 c         1488 g         2047 t
ORIGIN      
        1 aagaggcaaa gtatctggaa gaagatatca gaagatttct ttggttgcag gtctaacaaa
       61 tggtgagtta atcgctccaa tgacttacga agagatgatg acgagcgact tttttgaagc
      121 atggtttcag aagtttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgcaagattc catagaatgg gtaagttaga acttttatgc gaagagtttg ggcataaact
      241 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttacgagg ctcttttgtc
      361 ttgttcttgt ttcaattgac tatatatcac ggatacagtt gggaaagaag ctattaaaca
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaatgga
      481 tctgagtcaa acgatgcctt cttatgctca agtagtgagt ctttatgatg atacaagtat
      541 ctctgttagt agtaatgaga cggacaaagt gttggctggc tccctctata ctgatacaaa
      601 tgaacaggga ttaacgattc caagcagttt actaaaaaat tggaatgaac agacaggaaa
      661 aaatttgaca gctaatgatc ttattggcaa atcagtctca gccagcattg tagaaagtgc
      721 tgccgaaact agtaagattg ctcaatttca aacgaagatt gtacgtgtaa tcaatgatga
      781 agatgacatg gaggacagca acagtttcat gctgtctcat caaatggaaa cgattttgaa
      841 agaggctgga tttacgaaag ctgtatctta ttttatcttg gaactcaaag atccatcaca
      901 gacaaaagta gtaacagaag aattacagaa aaataagaag tatactgtgc tttctcaaca
      961 gagagttctt gatattgtga ttatctttat tcgtgttatt cagggattat tgattgtgct
     1021 ttcatcacaa gctattgtgg tagcagcggt tatgattggt atcattattt acatcaatat
     1081 catgcagcgt tccaaggaaa taggtgtcat gaaggcagtt ggttatcaga atcgtgatgt
     1141 caaaggaatt tttatttacg aggctatctg gattgtaggc atcgccttgc tgctggcatt
     1201 tttggttgca caaggggtgg gaagtttggc gaatgcgatt gtaagtcact tttacccatc
     1261 catcactaaa gtttttgaat taaatctttt atctgtttta ggaactctag ttttcgctct
     1321 attacttggt tatgtctcag cctacttcta cttggttatg tctcagccta cttcccggcg
     1381 cgtaagatta gtaaaatgga tcctgtagaa tcgttacgct atgaataata agggattcct
     1441 tgatttacag tcttaaaaag aaactcacac agaaaaacaa tggccatact tgcaattcaa
     1501 ctcaaatagt tgtataatag gtttgttgaa aggtgatttg tagtgattca agttactctt
     1561 ttcacaataa aaatttcatg attttcataa ggaggaaatc actaatggta gttaaagttg
     1621 gtattaacgg tttcggacgt atcggtcgtc ttgctttccg tcgtatccaa aacgtagaag
     1681 gtgttgaagt tacacgcatc aacgacctta cagatccagt tatgcttgca cacttgttga
     1741 aatacgacac aactcaaggt cgtttcgacg gtactgttga agttaaagaa ggtggatttg
     1801 aagttaacgg taaattcatc aaagtttctg ctgaacgtga tccagaacaa atcgactggg
     1861 ctactgacgg tgtagaaatc gttcttgaag ctactggttt ctttgctaag aaagaagcag
     1921 ctgaaaaaca ccttaaaggt ggagctaaaa aagttgttat cactgctcct ggtggaaacg
     1981 acgttaaaac agttgtattc aacactaacc acgacgttct tgacggtact gaaacagtta
     2041 tctcaggtgc ttcatgtact acaaactgct tggctccaat ggctaaagct cttcaagaca
     2101 actttggtgt tgttgaagga ttgatgacta ctatccacgc ttacactggt gaccaaatga
     2161 tccttgacgg accacaccgt ggtggtgacc ttcgccgtgc tcgcgctggt gctgcaaaca
     2221 tcgttcctaa ctcaactggt gctgcaaaag ctatcggtct tgtaatccca gaattgaatg
     2281 gtaaacttga tggatctgca caacgcgttc caactccaac tggatcagtt actgaattgg
     2341 tagcagttct tgaaaagaac gttactgttg atgaagtgaa cgcagctatg aaagcagctt
     2401 caaacgaatc atacggttac acagaagatc caatcgtatc ttcagatatc gtaggtatgt
     2461 cttacggttc attgtttgac gcaactcaaa ctaaagttct tgacgttgac ggtaaacaat
     2521 tggttaaagt tgtatcatgg tacgacaacg aaatgtcata cactgcacaa cttgttcgta
     2581 ctcttgaata cttcgcaaag attgctaaat aattcttgag ttgatagaaa gcaaggcttt
     2641 gtggtcttgc ttttttatat ggaaaaatgg atgacacgat catccattct tttttaattc
     2701 tttttcaaat gtatttgaaa gggtagtgaa agttagcctc tctaaagtaa gtgggtgggt
     2761 aaaggaaagt cggaaggcat gaagcataag ccggcttgtc tttgatttac tattatagag
     2821 agggtctccc aggataggaa gattatgatg caaaaggtgc acacgaatct gatgggttcg
     2881 ccctgtcttt agcttgcaat gagccaagga agtcttgttt gagaattgct ttaatctgct
     2941 tacatgcgtt tcagcatatt gcccattttt tgcatcaact attctttttc tacgatcatg
     3001 gcgatcacgt ccaattttgt ctctgaaaac aagttctttt ctgttgatat ttccatcaac
     3061 tagagcccaa tattctctag aaatctcttt tttctccaat aagcgattga gaatgggcag
     3121 gataaaagga tttttggcaa agagaactaa gccactggtt tccatgtcca gacgatgaac
     3181 gacatagcag gtttggccaa cataggtact gacatggtta agaagggaaa tttcgtttgg
     3241 ttgattacca tgcgttttca tcccctctgg tttgtttaca ataatcaagt gttgatcttg
     3301 ataaacttcc tgcactaagt ctgggttgcc ccaagggatc gtcttttggg aataatcttc
     3361 ctcgtcaaaa gtcaactggc aaacatctcc aggatttacg atttccttcc agtggacttc
     3421 ttcttgattt atcaaaatat gtttcttgat tctcaaaaaa tgacggattt ttctagggat
     3481 gaggagttgt tcctcaagta attgctttac tgtcatttga ggtagagagg cgggtaatgt
     3541 aaatgtgaat tgcatacaga tattgtaaca aaaaaagccc tatttggata ggaaatagct
     3601 aaattcttgt cttcctatga tgaagatgat aaaataaacg catgaaatta gataaattat
     3661 ttgagaaatt tctttctctt tttaaaaaag aaacaagtga actagaggac tctgattcta
     3721 ctatcttacg tcgctctcgt agtgatcgaa aaaaattagc ccaagtaggt ccgattcgaa
     3781 aattctggcg tcgttatcat ctaacaaaga ttatccttat actaggtttg agtgcaggct
     3841 tgctagttgg aatctatttg tttgctgtag ccaagtcgac caatgtcaat gatttgcaaa
     3901 atgccttgaa aactcggact cttatttttg accgtgaaga aaaagaggct ggtgccttgt
     3961 ctggtcaaaa gggaacctat gttgagctga ctgacatcag taaaaacttg cagaatgctg
     4021 ttattgcgac agaagaccgt tctttctata aaaatgacgg gattaactat ggccgttttt
     4081 tcttggctat tgtcactgct ggacgttcag gtggtggctc taccattacc caacagctgg
     4141 ctaaaaacgc ctatttatcg caggatcaaa ctgttgagag aaaagcgaaa gaatttttcc
     4201 ttgccttaga attaagcaaa aaatatagta aggagcaaat tctaaccatg taccttaaca
     4261 acgcttattt tggaaatggt gtgtggggtg tagaagatgc gagtaagaaa tactttggag
     4321 tttctgcatc agaagtgagt ctggatcaag ctgcgactct ggcagggatg ctcaaggggc
     4381 cggaactgta taatcccttg aattccgtag aagattctac taatcggcgc gatactgtct
     4441 tgcagaatat ggttgcagca ggatatattg ataaaaacca agaaaccgaa gctgctgaag
     4501 ttgatatgac ttcgcaattg cacgataagt atgaaggaaa aatctcagat taccgttacc
     4561 cctcttattt tgatgcggtg gttaatgaag ctgtttccaa gtataatcta acagaggaag
     4621 agattgtcaa taatggctac cgcatttaca cagagctgga ccaaaactac caagcaaata
     4681 tgcagattgt ttatgaaaac acatcgctat ttccgagggc agaggatgga acgtttgctc
     4741 aatcaggaag tgtagccctc gaaccgaaaa cagggggagt tcgtggagtt gtcggtcaag
     4801 ttgctgacaa tgataaaact ggattccgga atttcaacta tgcaacccaa tcaaagcgta
     4861 gtcctggttc tacaattaag cctttagttg tttatacgcc agcagttgaa gcaagctggg
     4921 ctttgaataa gcagttggat aaccatacca tgcagtatga cagctataag gttgataact
     4981 atgcagggat caaaacgagt cgagaagttc ctatgtatca agccttggca gaatcgctta
     5041 atctacctgc tgttgccact gttaatgatt tgggtgtcga caaggctttt gaggcaggcg
     5101 aaaaattcgg actcaacatg gaaaaggtcg accgtgttct tggtgtcgcc ttgggaagcg
     5161 gtgttgaaac caaccctctt caaatggctc aagcatacgc cgcctttgca aatgaaggtt
     5221 taatgcctga agctcatttt attagtagaa ttgaaaatgc tagtggacaa gttattgcga
     5281 gtcataaaaa ttcacaaaaa cgggtgattg ataagtctgt agctgacaag atgaccagta
     5341 tgatgttggg gactttcacc aacggtaccg gtattagctc atcgcctgca gactatgtca
     5401 tggcagggaa aactggaaca actgaagcag ttttcaatcc ggagtacaca agtgaccagt
     5461 gggtaattgg ttatactccg gatgtagtga ttagccactg gcttggcttt ccgaccactg
     5521 atgaaaacca ctatctagct ggctctactt caaacggtgc agctcatgtc tttagaaaca
     5581 ttgccaatac cattttacct tatacgccag gaagtacctt tacggttgaa aatgcttata
     5641 agcaaaatgg aattgcacca gccaatacaa aaagacaagt acaaaccaat gataatagcc
     5701 agacagatga taatttgtct gatattcgag ggcgtgcgca aagtctagta gatgaggcta
     5761 gccgggctat ctcagatgcg aagattaagg aaaaggctca aacaatatgg gattcgatag
     5821 tcaatctatt tcgctaagat gcttgtcaaa gcctagcttt cttgttataa tggataagat
     5881 ggaggcgtta tggcactaaa aaaagcaagc ctagcttgtg cggtttgtgg ttcgagaaac
     5941 tattcaatca agatcagcgg aaacctcaag cctacacgac tagaagtaaa taaattttgt
     6001 aagcattgtg gcaagtacac tacacacaga gaaacgagat aggagagagc gatgcgtttt
     6061 attggagata tttttagact tcttaaagac acaacatggc caactcgcaa ggaaagctgg
     6121 agagattttc gttctatcat ggaatacaca gctttctttg tagtaattat ttacattttt
     6181 gaccagttga ttgtttcagg tttgattcga tttattaaca ttttttagaa gattagtgga
     6241 gttaattaca ctagaaatct tctatttatg aaaggaaata tcatggatag ttttgataaa
     6301 gggtggtttg ttttacaaac ttattctggt tatgaaaata aggtgaaaga aaatctatta
     6361 caacgtgcac aaacctacaa tatgttggat aatattctac gcgttgaaat tccaacacaa
     6421 acagtgcaag ttgaaaaaaa tggaaagaga aaagaagtag aagaaaatcg ctttccaggt
     6481 tatgttcttg tagaaatggt catgacagat gaagcttggt ttgttgttcg aaacacacca
     6541 aatgttacag gatttgtcgg atcacacggg aacagatcaa aaccaactcc attattggaa
     6601 caagaaattc gcgacatttt ggtatctatg ggacaaactg ttcaagaatt tgatttcgat
     6661 gttgagattg gtcaaactgt acgtattatt gatggtgctt ttgcagacta cactggtaag
     6721 attacagaaa ttgataataa caaagtgaaa atgattatct ctatgtttgg taatgacaca
     6781 gttgcagaag taaatctaaa ccaaatcgca gaattataac cctgagagag gctggagctt
     6841 ctcttttttg tgcagtttag gagctaaagg gaa
//
LOCUS       DAVMEF010000103         6864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMEF010000103 DAVMEF010000000
VERSION     DAVMEF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.103"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            159..314
                     /locus_tag="VYQ30_001498"
     CDS             159..314
                     /locus_tag="VYQ30_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966582.1"
                     /translation="MEKLQFFLSQRACGCWKQAEKAVKLGSSETREENIILKGVPFIA
                     QPVTGDI"
     gene            449..1810
                     /gene="trpE"
                     /locus_tag="VYQ30_001499"
     CDS             449..1810
                     /gene="trpE"
                     /locus_tag="VYQ30_001499"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1966583.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKSHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYLYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGAMDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKSTLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1807..2373
                     /locus_tag="VYQ30_001500"
     CDS             1807..2373
                     /locus_tag="VYQ30_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1966584.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            2384..3388
                     /gene="trpD"
                     /locus_tag="VYQ30_001501"
     CDS             2384..3388
                     /gene="trpD"
                     /locus_tag="VYQ30_001501"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1966585.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3385..4152
                     /gene="trpC"
                     /locus_tag="VYQ30_001502"
     CDS             3385..4152
                     /gene="trpC"
                     /locus_tag="VYQ30_001502"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1966586.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKKLYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4139..4738
                     /locus_tag="VYQ30_001503"
     CDS             4139..4738
                     /locus_tag="VYQ30_001503"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1966587.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGSDYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4716..5939
                     /gene="trpB"
                     /locus_tag="VYQ30_001504"
     CDS             4716..5939
                     /gene="trpB"
                     /locus_tag="VYQ30_001504"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1966588.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5932..6708
                     /locus_tag="VYQ30_001505"
     CDS             5932..6708
                     /locus_tag="VYQ30_001505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1966589.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(6680..6781)
                     /locus_tag="VYQ30_001506"
                     /pseudo
     CDS             complement(6680..6781)
                     /locus_tag="VYQ30_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         1967 a         1381 c         1710 g         1806 t
ORIGIN      
        1 aaccaatacc tacggcaagg cgacgttgac gcggtttgaa gagattttcg aagagtataa
       61 aggtgtaccg agatgaacat ctcttcatca atgtctctgg tcgtggaggc aaggacctag
      121 ctgcgattgc agactaccta gaagctaaaa aataatagat ggaaaaatta cagttctttc
      181 tctcacagag agcttgtggt tgctggaaac aagcagagaa agctgtaaag ttgggttcat
      241 ctgaaacgag agaagaaaac atcattctca agggagtgcc ctttatcgca cagcctgtta
      301 caggagatat ctgagacagg aatgagagat aggagaaatc ctataattga ggtggcaccg
      361 cgaatttcgt cctcacgcaa gttattttgc gtggggattt ttcatacagt cgctacgaac
      421 tagaattaga actgaaaggg aaattacaat ggaacgaatc attcatggag atgtcttatc
      481 accaatcttg gcttatatgc gcttaaaggg gcaacacaag gttatcttag agagtattcc
      541 gagagacaag gaaacagctc gtttttctat cctagcctat aatcctgttt ttgagattaa
      601 gtttgaaaat ggagttcttt atcaaaatgg tcaagtgatt gatcgggatc ccttggattt
      661 cctttatgaa gtgattcata agagtcagca ccattcagag ctaccttttg gtggtggagc
      721 cattggtttt gtgggttacg atatgatttc gctttatgaa gaaattggtc aaatccctga
      781 ggatacaatt gggacgccag acatgcattt ctttgtctat gagagctata tggtctttga
      841 ccacaagaag gagaagatac atgtcataga ggatgcactt tatagtgagc gcagtcaaga
      901 agccttggaa aaatccttga accaagtgct tgaggaatta cgcattcctg ctccaaatga
      961 atttgaagac ttggatctat ctccgttaga cttcaaatcc catatcgctc ctcagaagtt
     1021 tgaggaaatg gtggaaaccg ctcgcgactt gattcgtaat ggcgatatgt tccaatgtgt
     1081 actcagtcag cgcttctcag cagaagttac tggaaatcca tttgacttct acagaaatct
     1141 ccgcgtgacc aatccatcca attacctcta tttatatgac tttggggatt atcaaatcat
     1201 cggtgccagt ccagaaagtt tggtttctgt caaaaatggc atcgtgacaa ccaatccgat
     1261 tgcagggacg cgaccaagag gggctatgga tgaagaagac aaggccttgg cgactgacct
     1321 cctctctgat gagaaggaaa cagcagaaca tcgaatgttg gtagacttgg ggcgtaacga
     1381 tattggccgc atctctgaaa cggccagtgt ccaagtcact aagtatatgg aagtggagct
     1441 cttccgttat gttatgcatc tgaccagcgt ggtcaagggg cgtttgcttc cagaactcac
     1501 tgctatggat gccttgaaat caacgcttcc agctggaacc gtttctggag caccaaaaat
     1561 tcgagctatg agacgcatct atgaactgga aacggaaaaa cggggcgtat atgcaggagc
     1621 aatcggctac ttgtctgcga cgggtgatat ggatttggcc attgccatcc gaaccatgat
     1681 tctcaaaaat caaagagcct atgtgcaggc tggggcaggg attgtctacg actctatcgc
     1741 ccaaaacgaa taccaagaaa ccattaacaa agcaaaatct atgactagaa ttggagaact
     1801 aagaccatga ttttattgat tgacaactat gattctttta cctataactt ggcccaatac
     1861 attgggaatt ttgcagaagt tcaggttttg agaaatgatg attccaagct gtatgaagaa
     1921 gctgaaaaag cagatggtct ggtcttttct cctggtcctg gttggccagt tgatgctgga
     1981 aagatggaag acatgattcg tgattttgca ggcaagaagc cgattctagg gatttgtttg
     2041 ggccaccaag ccattgcaga agtctttggt ggtaagctag gtttggctcc aaaagtcatg
     2101 catgggaaac agagcaatat caactttgaa gcgccatctg ttttgtatca aggtattgag
     2161 gatggccgtg cggtcatgcg ttatcacagt attttgattg aggaaatgcc agaagacttt
     2221 gaagtgacag ctcgttcgac tgatgaccaa gccatcatgg ggattcaaca taaaaaccta
     2281 ccgatttatg gcttccagta ccatccagag agcattggaa cgccagacgg cttgtcttct
     2341 attcggaatt ttatcgagga ggttgtaaag tgaggaaact aggatgaaag agattattga
     2401 aaaactagca aaatttgaaa atttatcagg tgtggaaatg acggatgtca ttgagcgtat
     2461 cgtaactggg cgtgtaactg aagcgcagat tgcttctctc ctcttagctc ttaagatgaa
     2521 gggggaaaca cctgaagaac gcacagccat tgcccaagtc atgagaggac atgcccagca
     2581 tattccaact gagattcatg atgccatgga caactgtggt acaggtgggg acaagtcttt
     2641 cagttttaat atttccacaa ctgcagcctt tgtcttggct ggtggcggta ttcacatggc
     2701 caagcatggt aaccgctcga tttcttctaa atctggttcc gcagatgtcc tcgaagcctt
     2761 gggaatcaat cttgacctca aaccagctga actaggtaag gtctttgata aaactggaat
     2821 tgtctttctc ttcgctaaaa atatgcaccc agctatgaaa tacatcatgc cagctcgttt
     2881 ggaattggga attccaacga ttatgaactt gactggtcca ctgattcatc caatggcttt
     2941 agaaacacag cttcttggaa ttagtcgtcc agaactccta gaaagtacag ctcaggtttt
     3001 gaaaaatatg ggtcgcaaac gtgccatcgt ggttgctgga ccagaagggt tggatgaagc
     3061 tggcttgaac ggaacaacca agattgcact tcttgaaaat ggcgaaatca gcttgtcaag
     3121 ctttactcca gaggatttgg gaatggaagg ctatgctatg gaagatattc gtggtgggaa
     3181 tgctcaggaa aatgcagaaa ttttgcttag cgttctgaaa aacgaagcaa gtccattctt
     3241 ggaaacgaca gtcttgaatg ctggtcttgg tttctatgct aatggtaaga ttgatagcat
     3301 caaggaagga gttgccttgg ctcgtcaagt gattgctaga ggcaaggccc ttgaaaaact
     3361 cagactgtta caggagtacc aaaaatgagt caggaatttt tagcacgaat cttagagcag
     3421 aaggcgcgtg aggtggagca gatgaagctg gagcaaatcc agcctctgcg ccagacctat
     3481 cgcttggcag aatttttgaa gaatcatcag gaccgcttgc aggtaatcgc tgaggtcaag
     3541 aaagctagcc ctagtttggg agatatcaat ctcgatgtgg atattgtgca acaggcccag
     3601 acttatgaag aaaacggagc agtgatgatt tcggtgttga cagatgaggt tttctttaaa
     3661 gggcatttgg attatctacg ggaaatttcc agtcaggtag agattccgac gctcaacaaa
     3721 gactttatca tagatgaaaa gcaaatcatc cgcgctcgca atgcaggtgc gacagttatc
     3781 ttgcttattg tggcagcctt gtccgaagaa cgcctcaaga aactgtatga ctacgcgaca
     3841 gagcttggtc tggaagtctt agtggagact cacaatctag ctgaactaga ggtagcccac
     3901 agacttggtg ctgagattat cggggtcaac aaccgcaact tgaccacctt tgaggtcgac
     3961 ttgcagacca gtgtagattt agccccttac tttgaggaag gtcgctatta catttctgaa
     4021 tctgccattt tcacagggca ggatgcggaa cgactagccc catactttaa cggaattttg
     4081 gtagggacag ctcttatgca ggcagaaaat gtggcccaga gaatcaagga gttgcagatt
     4141 gacaaaggtt aaaatttgtg gactatcgac caaagaagcg gtggaaacag ccgtttcagc
     4201 aggatccgac tatatcggtt ttgtctttgc acctagtaaa agacaggtga ctttagaaga
     4261 ggcagctgag ttggcaaagc ttattcctgc agatgtgaaa aaggttggag tatttgtttc
     4321 accaagtcgg gtagaactgc tggaagcgat tgacaaagtt ggcttggact tggttcaagt
     4381 tcacggtcag gtggcagatg atttatttga gaatttgcct tgtgccagta ttcaggctgt
     4441 gcaggtagat ggaaatgggc atgtccccaa ttctcaggca gattatctac tctttgatgc
     4501 ccctgtggca ggaagtggcc agccctttga ttggggtcaa ctggatacga ctggactagc
     4561 acagcccttc tttatcgcag gtggccttaa tgaagataat gtagtaaaag caattcaaca
     4621 ttttactccc tatgcagtag atgtatcgag cggagtggag acagatggac aaaaagatca
     4681 tgaaaagatt agaagattta tagagagggt aaagcatggc atatcaggaa ccaaataaag
     4741 atggatttta cggaaaattc ggcggacgtt ttgtcccaga aacattgatg acagcagttt
     4801 tggagttgga gaaggcctac cgtgaaagtc aggcagaccc aagtttccaa gaggaattaa
     4861 accagctttt gcgccagtac gtgggacgtg aaactcccct ttactacgca aaaaacttga
     4921 cccagcatat cggcggagcc aagatttatc tcaaacgtga agaccttaac catacaggag
     4981 cccacaagat taacaatgcc ttgggacaag tttggcttgc caaacgcatg ggtaaaaaga
     5041 aaattatcgc agaaactggt gctggtcagc acggtgtggc aactgcaact gctgcggccc
     5101 tctttaacat ggaatgtacc atctacatgg gtgaggaaga tgtcaaacgc caagccctca
     5161 atgtcttccg tatggagctt ttgggagcta aggtcgaggc cgtgacagat ggttcgcgcg
     5221 tgctcaagga tgcggtcaat gcagcccttc gttcatgggt ggctaatata gatgataccc
     5281 actatatcct tggttctgcc ttggggcctc atccatttcc agaaattgtt cgtgacttcc
     5341 aaagtgtcat tggtcgagag gctaaacaac agtaccgtga cttgacagga caaaatctgc
     5401 cagatgccct agtagcctgt gttggtggtg ggtcgaatgc catcggtctc ttccatccct
     5461 ttgtagaaga tgagtcagta gccatgtatg gagctgaagc ggctggattt ggtgtggata
     5521 cggagcacca cgcagctacc ttgaccaagg gtcgtccagg tgtccttcac ggttccctca
     5581 tggatgtgct ccaagatgcc catggtcaaa ttcttgaagc cttctctatc tcagcaggtt
     5641 tggactatcc tggtatcggt ccagagcatt ctcactacca cgatattaaa cgtgccagct
     5701 atgtccctgt gacagacgaa gaagccttgg aaggattcca actcttgtct cgtgtggaag
     5761 ggattatccc agccttggaa tctagccatg ctatcgcctt tgcggtgaaa ttggccaaag
     5821 aacttggacc agaaaagtct atgattgtct gcctatcagg tcgtggggac aaggatgtgg
     5881 ttcaagtcaa agaccgcttg gaagcagatg cagcaaagaa gggagaagct catgcctaag
     5941 acactaacag aaaaattgaa tgctattaaa gcagctggaa aaggaatttt tgttccctat
     6001 atcatggctg gtgaccacga gaaaggtttg gacggtcttg ctgaaacaat tcacttttta
     6061 gaagatttgg gtgtctctgc aattgaagtg ggtattccct tttcagaccc tgttgcagat
     6121 ggacctgtta tcgaagaagc tggcttgcgc agtttagccc acgggacctc tacccaggct
     6181 ttagttgaaa ccttgaaaac cattgaaaca gagattccac tggtcatcat gacctacttc
     6241 aaccccctct ttcagtacgg tgtggagaac tttgtcaaag atctggctga tacagctgtg
     6301 aagggcttga ttatcccaga cttgcctcat gaacatgcca actttgtaga accatttttg
     6361 gcagatacag atatcgcctt gattccttta gtaagcttga ccacaggaat tgagcgccag
     6421 aaagagttga ttgaaggggc ggagggattc atctatgccg ttgccatcaa tggagtgaca
     6481 gggaaatcag gcaattaccg tgcagatttg gacaagcact tggcgcaact tcatcaagtg
     6541 gccgacatcc cagtcttgac aggttttggt gtatctagtc aagccgatct ggaacgcttc
     6601 aatgcggtgt cagatggtgt tatcgtcggt tcgaaaatag taaaagctct ccaccaagga
     6661 gagccgattc aggactttat caaacaagca gtagcttacc aaaaataatc aaacaagtag
     6721 caagtaagag gaaaggcacg aaaggaagtc tttccttttt cttttgcagg cgaaaggcta
     6781 gttttatagt gaattgaatc tggaatatta cactacggtt cctaaaatat tttaagaaag
     6841 tgatttgaat ttcccaataa attt
//
LOCUS       DAVMEF010000104         6832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMEF010000104 DAVMEF010000000
VERSION     DAVMEF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.104"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            158..346
                     /locus_tag="VYQ30_001507"
     CDS             158..346
                     /locus_tag="VYQ30_001507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966590.1"
                     /translation="MTWFEEIFEEHQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFT
                     IDYTDDELLDKEAVFTTH"
     gene            489..662
                     /locus_tag="VYQ30_001508"
                     /pseudo
     CDS             489..662
                     /locus_tag="VYQ30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            699..1151
                     /locus_tag="VYQ30_001509"
     CDS             699..1151
                     /locus_tag="VYQ30_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1966591.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            1442..1912
                     /locus_tag="VYQ30_001510"
     CDS             1442..1912
                     /locus_tag="VYQ30_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1966592.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEEGEFYPYPKY"
     gene            1931..3019
                     /locus_tag="VYQ30_001511"
     CDS             1931..3019
                     /locus_tag="VYQ30_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1966593.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            3000..3158
                     /locus_tag="VYQ30_001512"
     CDS             3000..3158
                     /locus_tag="VYQ30_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV1966594.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            3269..4303
                     /locus_tag="VYQ30_001513"
     CDS             3269..4303
                     /locus_tag="VYQ30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1966595.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            4305..6548
                     /locus_tag="VYQ30_001514"
     CDS             4305..6548
                     /locus_tag="VYQ30_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV1966596.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDGVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            6599..>6832
                     /locus_tag="VYQ30_001515"
     CDS             6599..>6832
                     /locus_tag="VYQ30_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1966597.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLI"
BASE COUNT         2241 a         1017 c         1440 g         2134 t
ORIGIN      
        1 tagaataatt gatgtttttg aagtagtaaa tcgcctcctt gtaataactg ttgaaaatcc
       61 tgatttcgaa gacttgagag ttaatcatac tcaatgaaaa tcaaagagca aactaggaaa
      121 ctagccgcag gctgtacttg agtacggtaa ggcgacgttg acgtggtttg aagagatttt
      181 cgaagagcat cagtttgtaa aaattggaga taaaaaatat agagtacgca gtggtcctat
      241 gattcattca actccgcctc aatctgtctt agatcgagat acttttacaa ttgactatac
      301 agatgatgaa ttgctcgata aagaagcagt ttttactact cattagaaaa ataggtctga
      361 ataaattggt ttcccttaga accacatcta ggggctttta tgtataatag aataataagt
      421 atcactcctt tacaatacgt gtaacacgtg ttgtaatggt gttgtcagga ggtaaaagcg
      481 ctatacctat gacacaaaaa gagatggttt aactccttac ggcccatggt tggataaaga
      541 ctagaggcgg taaaggctcc catattaaaa tcgagaagca aggggaaaga cctatcacaa
      601 tccttcatgg tgagctgaat aagtacactg aaagagggat aggaaagcaa gctgggttgt
      661 aaaattcagt ccctgctttt tgatatggag gtagtgaaat gttagttacg tatccagctt
      721 tattttatta tgacgacaca gacggaacag aagcgactta ttttgtccat ttcccagatt
      781 ttgaatactc agctacacaa ggagagggga tttctgaggc tttggctatg gggtcggagt
      841 ggctagggat aactgttgca gatttgatag agagtgatgg agagttacct cagccatcag
      901 atatcaatag cttgtcttta attgataatg atccttttaa agatgatgaa gatttcgtgt
      961 caacctatga ccttgataaa tcttttattt ctatggtatc tgttgatgta tcagaatacc
     1021 taggaagtca ggaacccatt aaaaagactt tgaccatacc aaaatgggca gataagttgg
     1081 gacgagaaat gggacttaac ttttctcaga ctttgacaga cgctattgca gataagaaag
     1141 ttcaagccta agattaagcg tggtatcgtg gtaatagtca ataaaaaagc acgtccactt
     1201 gtgctagtta ctcgcctatt gattttatag atttattagc cctttcgagg gctttttata
     1261 ttgattttta tgagaaatga agaaatcaga cttttaagaa aatcactgat accaagggtt
     1321 taaatgaggt aatatggtat aattaggaca taaaataatt ttgtggtaag atggtagtat
     1381 ctattttagc atatttccga gcaatcgggg cgattaaaga gtcgcataga aagaggacaa
     1441 aatggcaaca tacggatttt tagatatttt agaggaagag ttggacaaga actttccctt
     1501 tgactttgag attagttggg acaaacgcaa ccatgcggta gaagtgagtt ttctattgga
     1561 agcgcaaaat gctgcaggtg tggagatggt ggatgaagac ggagaggttt catcagatga
     1621 cattcttttt gaggaagcgg tccttttcta caatcctgcc aagtcaacag tcaatgagga
     1681 agactatttg actgttattc cttacctgcc taaaaaagga ttttcccgtg aatttttagc
     1741 ttattttgcg cttttcctta aagatactgc tgaggttggg ctagatgtcc tcatggactt
     1801 tttggaagac ccagaagcag aggaatttgt catggaatgg aaccaagaag tctttgaaga
     1861 aggaaaaatt ggcttggaag agggagaatt ttatccttat ccaaaatact aggagttggt
     1921 tggaggtgtt atgaagaaaa ttgggactta tttggtttat gtgctagctg ttgtctttat
     1981 tatgctagct tttgcttgtg gaacaatcgc atttgcagag ttggggtatt ccgcagtttt
     2041 agcctttact tttggttatg ccttcgctct tctaagcatg tatttaatct ttattcttca
     2101 tgagctgggt catgcttttt gtggctactt gacaggctat cggctggtgg cttttggatt
     2161 aggacatttt attttgacca aaaagtcagg caggtttcat cttagtagaa cagccattct
     2221 gaaaaatgtt ggtgctcaat acattggttt aaaagaggat gaaagcgatc aaagaatcat
     2281 cctgatgctt tcaggaggct tgatggttca tctcggcttg atattattgg cgatattgtt
     2341 tggatttttg acaagaagct ggtattttgc agggacttgg atttttctta atttatcttt
     2401 cttcctaaat aacattttgc cagtcgacat caccgatgga gcaaaaattt gggaattact
     2461 acaacaccct gaaaatacca aatacgccta cctggtattg agacattctg cccagacctt
     2521 gctagctcct caagaatatg atttgaaaga ctttgttcaa gcagttcctg aggatgcacg
     2581 agggagtttt gcggatggtg tattaggtat gcaaggtgaa gtttccattt tggaagggaa
     2641 ggaagaagta gctaaacagc agtttcaagc attacttgag aggacaacta ctccgatgat
     2701 gcagacagtt gcccaactat ccctgcttca tatcgccttg ttggaaggag attttgagca
     2761 agcggagcaa tatgctagta ttcgacaggt caaatcgttt ctgtctctca aactgtccaa
     2821 tcttcaagtg atacaagcct ggtatcaatt aaaggtgaag aaagatgtag atcgaactcg
     2881 taaggctctc aagattgcta gacagaaaat ggatagtagt cgaatgttga gggacgagaa
     2941 acgctattat gaaaacttgt tagcagagct ggaaaaggaa atgttagaag gaactgaaga
     3001 tggaactaga aatttctgat ttccaaggtt gcaagattgc cctcttttgt ggggataagc
     3061 tactgacgat tttgcgtgat gataaggcaa gcattccctg ggccaatatg tgggaactgc
     3121 caggtggggg aaagagtgtc agggaaaatc tgatatagtt ttgcattaaa agaatatcct
     3181 ttcaaggtta ttcttttttt aattttgtcc caaaataatt gactttgtcc cgaaaatata
     3241 gtattatttg tataaggagg aatgaatcat gaaaaaatca gatgttcttg atttaataaa
     3301 gtatcattat gagggcagag agacagaatt tagaaatcaa tccatagcaa ttgctcggaa
     3361 cttcaataaa catggtgata cacaaatagc acaatatatt atggggttaa tgtctcaaag
     3421 tgatagattc atgccacaaa tagaaaatcc tagtgagtat ttgactcctg ctaaactaga
     3481 tattggtcct ctcccattgc ctctttcaat tatgaatgat ttaaaaggaa taataaacgc
     3541 tgtcaatcat catattggaa taaataaatt tttatttgtg ggatctccag gtactggaaa
     3601 aactgaaagt gtaaagcagg tggctagact tattggcaag gaattacttg tagtagattt
     3661 cagtcactta gtagatagta agctagggca aacagtcaaa aacttagcaa ctctttttaa
     3721 tgaaatcaat aatcttcctt tcaagcaaaa ttatatcatc ctatttgatg aaattgattc
     3781 catagtgttg gatagggtta atcagaatga tttaagagaa atgggacgag tgacttctgc
     3841 ctttttaaag gagttggata ggctgtcacc agaaattgta ttaattgcaa caacaaatct
     3901 ttttgaaaat cttgataaag cagttacgag aagattcgat gccataattg actttgaccg
     3961 ttatactgat gaagataaag tagaagtagc tactattatt ttaaatgagt tattaaagca
     4021 atttaagaat gtagctagag atttaaaatt atttaaaaaa attatcaata gtgccaatgt
     4081 aataccgaat cctggtgatt tgagaaactc aataagaacg tcattggcat ttagtgaccc
     4141 atcagatccg catgattacc aaaaacgcct gctaagaagt ttgcataatg gtagaaattt
     4201 atctatttct aaattatcga agctaggttt tactgttaga gaaattgaga ttttgacagg
     4261 tatttctaag agtagtgtat cacgagagtt aagcgaggat taaaatgaat acattattga
     4321 cattacgagg taaaagtttt acaccaaagt cagcaaaggg gaggggaccg ataacgattc
     4381 ccgcagattc tattgttacg ctaaaccatt taaatgaact ttatacatcc cttgaacgag
     4441 taaaagaata ttggtctaat aacacaatca ttgatggagt acttattagt gtgtattata
     4501 atcgtgtcct agctaagagc aatcgtatcg atggtttttt taatgtgggt ggcggaaatg
     4561 caattccaaa tgaaacgatt gtgggtgcaa aatttagtaa tgataagagt aggcatatta
     4621 ttacacacta catttcaaag gaagtgttag ataagacaat tgagcatact aaaaaagtta
     4681 taacaatatt caaagattat tttgctagtg ctcccatgac gagtatggtg ttctccgact
     4741 cttcaaaatt taatcaaatt gattattcta agtatggtct gtcgaaatca aaattcaaac
     4801 aatacttaag agacgcttgc tttgtcaaag gatttggtat tgaacaggca gatatgtcta
     4861 ctatacgtaa ttctattgta acaatctacg atgtaaaaac taatctctta acattactta
     4921 aacaacttga tattgatgtt tcagaagcta acatcatgga ccagacaact gttttacttg
     4981 acgaaaaaaa tattgagtta ctactgctaa aagcacctta tctagtatca atgatagtag
     5041 aagatttttc tagcctgtca ccagagaatt tttataatga tgggaacact ttttataata
     5101 atctcccttt tccaactaat gaacctatta ttggtgtcat cgatacgttg tttgatgatg
     5161 gagtttattt caatgagtgg gtagagtatc atgatttaat ttcaccagat attagcaaag
     5221 gaagtcaaga ctataagcac ggtacagcag tttcctcggt aatagtagat ggagctagtt
     5281 tgaatccaaa actagatgac ggatgtggaa gatttagagt cagacatttt gggatatctt
     5341 tacagagtgg tttcaattcc ttcacaatta tcaaacagat tagagaaatt gtgtctcaaa
     5401 atcctgatat taaggtttgg aacctttcgc ttggatcaaa tgatgaaatt agggaaaact
     5461 ttatttccgt cgagggttcg ttgttagacg agattcaatt tgataacgat gtaattttta
     5521 ttattgcagg cactaatggg attgcccctg atggacaacg aaaaaagatt ggagcaccag
     5581 cagattcact caattcaatt attgtaaatt ctgttgattt tagcaatcaa gtcgtttctt
     5641 attcacgcga aggagttgtc ctgtctttct ttatcaaacc tgatgtttca tactatggcg
     5701 gggggaacgg agacttcata aatgtttgtg aaccactagg gctggctcga gtagctggga
     5761 ccagttttgc ggctccattt attgctagaa aaatggctta cctcattcat attatgggat
     5821 taagtcgtga agaagcaaag gctttattaa ttgattcagc gataccatgg aatgataaaa
     5881 aaacattcgc agacatttct ttacttggat atggggttgt gccaatcaaa atagaagata
     5941 ttttgtctac acctgatgat gaaattaaat ttatagtctc agatgtttct agagcatatg
     6001 aaacttataa ttatgatttc ccagttccaa tttctaaaga acactatcca tatgtagcaa
     6061 aggccactat gtgctatttt ccaaagtgtt ctagaaatca aggagttgac tatactaata
     6121 cggaaatgca acttagtttg ggagttttag ataagaaagg cattaaaaca attaataacg
     6181 acaatcaata ttctgaaaat gcaccaggat atgtgagaga aaagtcggca cgaaatattt
     6241 ttaggaaatg ggataatgtc aaacatttag gagaaaaatt ttcttctcgt aaaaaagcta
     6301 agccgattct gaatccttct aatcctcaat ggggaatgag tgtaaaaaca attgaacggt
     6361 taagaaatga tgatggttat ggtattcgtt ttggagtagt tgttactcta aaggagttga
     6421 atggtgtcaa tagaatcgaa gattttattc aacaagccga attacgcggt tggctagtta
     6481 atagattgca agtagaagct caagtagacc tctttaatag tttaaatgaa gaaattgaat
     6541 ttgaatagtg gatattttat ccttattcta gatcttagga acacaagagg gcaaagatat
     6601 gaatattact tatatagttg gaaatggttt ggatttgcaa tatggtttga aaacaagata
     6661 taaagatttc tatgaatttc aaaataggac ttacataagt aggaaagaga acgaggaaga
     6721 atattcaaat ctcatttatg agtctctatt ttctgataca gtaaatgatt atgaaaattg
     6781 gtcagatttt gaattgtcaa ttggtaaatt aacaaaagat aacgacctca tc
//
LOCUS       DAVMEF010000105         6724 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMEF010000105 DAVMEF010000000
VERSION     DAVMEF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.105"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..1085
                     /locus_tag="VYQ30_001516"
                     /pseudo
     CDS             1..1085
                     /locus_tag="VYQ30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1128..1769)
                     /gene="plsY"
                     /locus_tag="VYQ30_001517"
     CDS             complement(1128..1769)
                     /gene="plsY"
                     /locus_tag="VYQ30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1966598.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDFLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1905..3848
                     /gene="parE"
                     /locus_tag="VYQ30_001518"
     CDS             1905..3848
                     /gene="parE"
                     /locus_tag="VYQ30_001518"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1966599.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            4268..>6724
                     /gene="parC"
                     /locus_tag="VYQ30_001519"
     CDS             4268..>6724
                     /gene="parC"
                     /locus_tag="VYQ30_001519"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1966600.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVV"
BASE COUNT         2121 a         1298 c         1542 g         1763 t
ORIGIN      
        1 gtctcccaag tcgtacgata cttcttaact ggctagcaca atacagaaaa aacgggtata
       61 ctattgttga gaaaccaaga gggagagtac ctaaaatggg acgtaagcca agaacgagac
      121 ctgaagagag gacagaatta gaacgtcttc aagcagaaaa tgagtacctg agagcggaga
      181 atgccatcct aaaaaagtta agagaactcc gattgaagga ggaaaaagag aaagaagaaa
      241 gacagaaatt gttcaagaat taatgactga gttttcgtta gatattcttc taaaagccat
      301 taagctagct cgttggacct actactatca cttgaaacag ttagacaaac cagataagga
      361 ccaagagctt aaagctgaaa ttcaatccat ttttatcgaa cacaagggaa attatggtta
      421 tcgtcgaatt tatttagaac taagaaatcg tggttatctg gtaaatcata aaagagttca
      481 aggcttgatg aaagtactca atttacaagc taaaatgcga cagaaacgaa aataaaggag
      541 acgttggtaa gaaggcagag aatctcattc aaggccaatt tgaaggctct aaaacaatgg
      601 aaaagtgcta cacagatgtg acagaatttg ccattccagc aagtactcaa aagctttact
      661 tatcaccagt tttagatggc tttaatagcg aaattatcgc ctataatctt tcaacttcgc
      721 ccaacttaga acaagtgaag agtatgttag aacaggcctt tacagagaaa tactatgaga
      781 atacgattct ccatagtgac caaggctggc aataccgaca cgattcttat catcggttcc
      841 tagagagtaa gggagttcaa gcatccatgt cacgtaaggg taacagccca gacaatggta
      901 tgatggagtc cttctttggg attctgaaat cggaaatgtt ttacggttat gagaagtcgt
      961 ttcagtcgct taagcaattg gaacaagcca ttatagacta tattgattac tacaacaata
     1021 aacgaattaa gataaaacta aaaggactta gccctgtgca atacagaact aaatccttcg
     1081 gataaattaa ttgtctaact gtttggggtc agtacagaac tggcgtttta ttttttagga
     1141 tcttgatggg ttaggttcaa tccccaaggg accaaatttt cagttttatt tttgatacga
     1201 gctatattgt ccttatgacg aatgataatc aaactagcaa gtgctaagat aatagcgatg
     1261 aagagaaagt catagttact caggataaaa ccaaaaagtg gaaagagcag aaccccgata
     1321 acagccgcaa tcgatgctgt gacactagac agtgaaatca tactgccaag atagagagct
     1381 ccaaagaaga taatcgcaag gtagagacag aagataggcg caaatccgaa aatcactcca
     1441 gcactggttg cgacagcctt accaccttta aatcctgcaa agatagggaa ggtatggccg
     1501 ataacagcca aaagtccaaa gatgagagga gaaacgcctt gtagatgaaa aataatcgga
     1561 agcagcgttg ctagggttcc tttgaaaaag tcaatcacaa aggttgccat accagctttc
     1621 ttacctaaaa tgcggaaggt gttggtcgtt ccagtgttac cagaaccatg ctcgcgtaga
     1681 ttgatttgaa agaatacttg tccaatccag agaccagatg gaatcgaacc cagcagatag
     1741 gctaggatta ataaaactat tgtaatcata ctcctattat atcatgaatt gggaaagaaa
     1801 agcagagaat ctctgctgaa attgtcacat cggagaaaga aaaattttgc aaaatccttg
     1861 gaaaacctgt agaatagtaa agatgaacga ataggaggtt ccttgtgtca aaaaaggaaa
     1921 tcaatattaa caattataat gatgatgcta ttcaggtgct agaagggttg gatgcggtcc
     1981 gaaaacgtcc ggggatgtat attggatcga ccgatggcgc tggtcttcat cacctagttt
     2041 gggaaatcgt tgataatgca gtcgatgaag ccttgtctgg gtttggtgat cgtattgatg
     2101 taactatcaa taaagacggt agtctaacgg ttcaagacca tggacgtggg atgccgacag
     2161 gtatgcacgc tatgggaatt ccaactgttg aggttatctt taccattctt catgccggag
     2221 ggaaattcgg tcaaggtggc tataagacat caggtggact tcacggagtg ggttcttccg
     2281 ttgttaacgc cctttctagc tggttagaag ttgaaattac ccgtgatggc gcagtttaca
     2341 agcaacgttt cgaaaatggt ggaaaacctg tcacgacttt gaagaaaatc ggtacagcac
     2401 ccaagtctaa aacaggcacc aaagttactt ttatgcctga cgcgactata ttttctacga
     2461 cagatttcaa gtacaatacc atttcagagc gccttaatga atcagccttt ctcttgaaaa
     2521 atgtgacctt gtctttaacg gacaagcgaa cagatgaagc gattgagttc cactatgaga
     2581 atggagtaca agattttgtt tcttatctca acgaagataa ggaaatcttg acgccagttc
     2641 tttactttga aggggaagac aatggttttc aagtggaagt agccctccag tacaatgacg
     2701 gattctcaga taacattcta tcctttgtca ataacgttcg caccaaggac ggtggaacgc
     2761 acgagacagg actcaagtct gccattacca aggtcatgaa tgactatgca cgtaaaacag
     2821 gtcttctcaa ggaaaaagat aaaaaccttg aaggttcaga ctatcgtgag ggactagcgg
     2881 ccgttctttc tatcttagtt cctgaagaac acttgcagtt tgaaggacag accaaggata
     2941 aactaggaag ccccctagct cgcccagttg tggatggaat agtggctgat aagttgacct
     3001 ttttccttat ggaaaatggg gaattagctt ctaacctcat ccgcaaggct atcaaggccc
     3061 gtgatgctcg tgaagcagca cgtaaggcgc gtgatgagag ccgaaatggg aagaaaaaca
     3121 agaaagataa gggcttgttg tctgggaaat tgaccccagc ccaatctaag aatcctgcta
     3181 agaatgaact ctatctagtt gagggggact ctgccggtgg ttctgccaaa caaggtcgtg
     3241 accgcaagtt ccaggctatt ctacctcttc gtggtaaggt tatcaataca gccaaggcca
     3301 agatggcgga tatcctcaaa aatgaagaga tcaataccat gatttatacc attggtgcgg
     3361 gtgttggagc agacttctct attgaagatg ccaactatga taagatcatt atcatgaccg
     3421 atgcggatac cgacggtgcc catatccaga ccttgctctt gacatttttc taccgttaca
     3481 tgcgtccgct agtcgaggca ggtcatgtct atattgccct cccacctctt tacaagatgt
     3541 ccaaaggtaa aggaaagaaa gaagaagtgg cctacgcttg gacggacgga gaactagaag
     3601 aactccgtaa acagttcggt aaaggcgcta ccctccaacg atacaaagga cttggtgaga
     3661 tgaatgcgga ccagctctgg gaaacaacca tgaacccaga aacacgtacc ctcatccgtg
     3721 tcacaattga agatttagcg cgcgccgaac gccgcgtcaa tgttctcatg ggagataagg
     3781 tagaaccacg ccgtaaatgg attgaagata atgtcaagtt tacgctagaa gaagcgacag
     3841 tgttttaatg aaagaaaata atatgttagt aaactggaaa ttagagtcgg atgtgaatga
     3901 ctatgtgaaa aagcagtttg aaaatcttgg tttgaaaaaa ttgcaggatt attaatgaag
     3961 aatctgctat gagtgcttat ctcaaagagg cgcttaaggg agcggctaag acacaaacta
     4021 agactaattt tggaaaacca gattttcata ttgaaaaata caaacaggat agtttggtta
     4081 cttatcgtaa ctgaacacgg gaggaattcc cgtaaaatat cttttactgc ataaaaacct
     4141 actctacatt ctttgaaagg agttgaacac gccctagata ctgtgtgaaa aagataaatt
     4201 ctcttgtgag tttgcttact tcaagaattt tctattttca cttggtattt tatgggcttt
     4261 gtatcttatg tctaacattc aaaacatgtc cctggaggac atcatgggag agcgctttgg
     4321 tcgctactcc aagtacatta ttcaagaccg ggctttgcca gatattcgtg atgggttgaa
     4381 gccggttcag cgccgtattc tttattctat gaataaggat agcaatactt ttgacaagag
     4441 ctaccgtaag tcggccaagt cagtcgggaa catcatgggg aatttccacc cacacgggga
     4501 ttcttctatc tatgatgcca tggttcgtat gtcacagaac tggaaaaatc gtgagattct
     4561 agttgaaatg cacggtaata acggttctat ggacggagat cctcctgcgg ctatgcgtta
     4621 tactgaggca cgtttgtctg aaattgcagg ctaccttctt caggatatcg agaaaaagac
     4681 agttcctttt gcatggaact ttgacgatac ggagaaagaa ccaacggtct tgccagcagc
     4741 ctttccaaac ctcttggtca atggttcgac tgggatttcg gctggttatg ccacagacat
     4801 tcctccccat aatttagctg aggtcataga tgctgcagtt tacatgattg accacccaac
     4861 tgcaaagatt gataaactca tggaattctt acctggacca gacttcccta caggggctat
     4921 tattcagggt cgtgatgaaa tcaagaaagc ttatgagact gggaaagggc gcgtggttgt
     4981 tcgttccaag actgaaattg aaaagctaaa aggtggtaag gaacaaatcg ttattactga
     5041 gattccttat gaaatcaata aggccaatct agtcaagaaa atcgatgatg ttcgtgttaa
     5101 taacaaggta gctgggattg ctgaggttcg tgatgagtct gaccgtgatg gtcttcgtat
     5161 cgctatcgaa cttaagaaag acgctaatac tgagcttgtt ctcaactact tatttaagta
     5221 caccgaccta caaatcaact acaactttaa tatggtggcg attgacaatt tcacacctcg
     5281 tcaggttggg attgttccaa tcctgtctag ctacatcgct caccgtcgag aagtgatttt
     5341 ggcgcgttca cgctttgaca aagaaaaggc tgagaaacgt ctccatatcg tcgaaggttt
     5401 gattcgtgtg atttcgattt tggatgaagt cattgctctt atccgtgctt ctgagaataa
     5461 ggcggacgcc aaggaaaacc tcaaagttag ctatgatttt acggaagaac aggctgaggc
     5521 tatcgtaact ttgcaactgt accgtttgac caataccgat gtggttgtct tgcaggaaga
     5581 agaagcagag cttcgtgaga agattgctat gctggcggct attatcggtg atgaaaggac
     5641 tatgtacaat ctcatgaaga aagaacttcg tgaggtcaag aagaaatttg caactcctcg
     5701 tttgagttct ttagaagaca ctgcgaaagc aattgagatt gatacagcta gtcttatcgc
     5761 tgaggaagat acctacgtca gcgtgaccaa ggcaggttac atcaagcgta ccagtccacg
     5821 ttcctttgcg gcttccacct tggaagaaat tggcaagcgt gatgatgacc gtttgatttt
     5881 tgttcaatct gccaagacaa cccagcacct cttgatgttc acaagtcttg gaaatgtcat
     5941 ctacagacca atccatgagt tggcagatat tcgttggaag gacatcggag agcatctgag
     6001 ccaaaccatc acaaactttg aaacgaatga agaaatcctt tatgtggaag tactggatca
     6061 gtttgacgat gcgacaacct actttgcagt gactcgcctt ggtcaaatca aacgggtaga
     6121 gcgaaaagaa ttcactccat ggcggaccta tagatctaag tctgtcaagt atgctaagct
     6181 caaagacgat acagatcaga ttgtagcagt ggctccgatt aaactagatg atgttgtctt
     6241 ggttagtcaa aatggttatg ccctgcgttt caatatcgaa gaggttccgg ttgtcggtgc
     6301 taaggcagca ggtgtcaagg ctatgaattt gaaagaagat gatgtcctcc aatctggctt
     6361 tatctgtaat acttcgtcct tctatctctt gacccagcgt ggaagcttga aacgtgtttc
     6421 tattgaggaa attctagcaa ccagccgtgc caaacgagga ttacaagtct tgcgtgagtt
     6481 gaaaaacaaa ccgcatcgtg tcttcttggc aggagcagtt gcagagcaag gatttgttgg
     6541 cgatttcttc agtacggaag tggatgtgaa cgaccaaact ctgcttgtcc aatccaataa
     6601 aggaacaatc tatgaaagcc gattgcaaga cttgaacttg tcagaacgca ctagcaatgg
     6661 aagcttcatt tctgacacga tttcagatga agaagttttt gacgcttatc ttcaggaagt
     6721 agtt
//
LOCUS       DAVMEF010000106         6628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMEF010000106 DAVMEF010000000
VERSION     DAVMEF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.106"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1606
                     /locus_tag="VYQ30_001520"
     CDS             <1..1606
                     /locus_tag="VYQ30_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEV1966601.1"
                     /translation="NLIKNSNFEQGELFWNSRNRGIINSNESYSNGHYSGMIPSTAIN
                     GNRADGYIGQVIDIEPNEDYLVSAFAKVDVSGAEGYFTARWFDDNKQGEIVQNNIGEA
                     VDQTVNTTEWKKYMFSFNSGKHDKVLIQLVKWSEDDTTKKSNIFIDNVEMYQLSKGNS
                     YKKIWRDDFDGEQLNKKYWGYELGSIRGWEQQHYVRSDENVFLRSGNLVLRATNRSKE
                     DQYFNPRNNHRKVVYNSGSVRTHGKVEFLYGKLEMRAKLPKGQGVFPAFWTLGSDFTL
                     DGKINPVQGRGWPSTGEIDIMELVGERNSNGSGNKTVYQTLHYGQSDNDDGKFAGHGT
                     AFKLSSGNFNDSYHTFSIDWYKDYIVWMVDNHIVRKVYYGSDNIAKKIFNRPQYVQLN
                     LAMGGNWPGVVDNNLSGTEFVVDYVSYSRNDLQQHQAEEYYSQSPKINGAKDIVINRG
                     DVPDLLKGITTNSGYELDYSIENEPMFNNFGGNTSVDLLIRDKTEKEKISQMPPGVYN
                     LHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            1619..1936
                     /locus_tag="VYQ30_001521"
     CDS             1619..1936
                     /locus_tag="VYQ30_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1966602.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            1953..2270
                     /locus_tag="VYQ30_001522"
     CDS             1953..2270
                     /locus_tag="VYQ30_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1966603.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLSKEVVEVYKRF
                     EKN"
     gene            2280..3635
                     /locus_tag="VYQ30_001523"
     CDS             2280..3635
                     /locus_tag="VYQ30_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1966604.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            3958..5433
                     /locus_tag="VYQ30_001524"
     CDS             3958..5433
                     /locus_tag="VYQ30_001524"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1966605.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDIYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPYEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            5430..6278
                     /locus_tag="VYQ30_001525"
     CDS             5430..6278
                     /locus_tag="VYQ30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1966606.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            6293..>6628
                     /locus_tag="VYQ30_001526"
     CDS             6293..>6628
                     /locus_tag="VYQ30_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEV1966607.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILL"
BASE COUNT         2106 a          899 c         1435 g         2188 t
ORIGIN      
        1 aaatttaata aaaaattcaa attttgagca aggagagctg ttttggaact ctagaaatag
       61 gggaataatt aattcaaatg agagttatag taacggacat tactcaggta tgattccttc
      121 tactgcaatt aatggtaata gagcagatgg atatattgga caagttattg atatagaacc
      181 taatgaagat tatttagtat ctgcttttgc caaagtagat gttagtggag cagagggata
      241 ttttaccgca agatggtttg atgataataa gcaaggagag attgttcaaa ataacattgg
      301 agaagctgtt gatcaaacag ttaatacaac cgaatggaaa aaatacatgt tttctttcaa
      361 ttccggtaaa cacgataaag ttttgattca attagtgaaa tggtcagaag acgatactac
      421 aaaaaaatct aatattttta ttgataatgt agagatgtat caactgtcta aaggaaattc
      481 atataagaaa atttggagag atgattttga tggcgaacaa ttaaataaaa aatattgggg
      541 atatgaatta ggatcaattc gtggttggga acagcaacat tatgtaagaa gtgatgagaa
      601 tgtttttcta cgttcgggaa atttagttct tagagctacg aatcgctcta aggaagatca
      661 gtattttaac ccaagaaaca atcacagaaa ggttgtatat aattcaggaa gtgttagaac
      721 acatgggaaa gtagaatttc tttatggtaa attagagatg cgtgcaaaat taccgaaagg
      781 tcaaggagtt tttcctgctt tctggactct tggatcggat tttacacttg atgggaagat
      841 taatcctgtt cagggacgag gatggccgtc aacaggtgag atagatataa tggaattagt
      901 tggtgaacga aattcaaatg gaagtgggaa taagacagtt tatcaaacgc ttcattatgg
      961 acagtcggat aatgatgatg gaaaatttgc aggacatggt acagccttta aattatcgag
     1021 tggaaacttt aatgattcgt atcacacttt ttcaattgat tggtataaag attatattgt
     1081 atggatggtt gacaatcata ttgtcagaaa agtatattat ggatcagata atatagcgaa
     1141 aaagattttt aatagacctc agtatgttca gttaaattta gcgatgggag gaaattggcc
     1201 cggagtagtg gacaataatt tatcaggaac agaatttgta gttgattatg tttcttactc
     1261 tagaaatgat ttacaacaac atcaagctga agagtattat agtcaatctc ctaaaataaa
     1321 tggcgctaaa gatatagtaa taaacagagg agatgttcct gatttattaa aaggaattac
     1381 aactaatagt ggatatgagc tagattattc tattgagaat gaacctatgt ttaataactt
     1441 tggagggaat acttcggtag atttattaat tagagataaa actgaaaagg aaaagatttc
     1501 acaaatgcct ccaggagttt ataatttgca ttatactagt cgtcccaaac atttagatta
     1561 tgagtctaaa gttgatcgaa aaacagtaac attgacaata aaataaaagg agtaagctat
     1621 ggctaaagta acaattatgt tagcatgtgc agcaggtatg agtacaagtc tgctagtgac
     1681 aaagatgcaa aaggcagcag aagataaggg gttggatgca gaaatttttg cagttccagc
     1741 tcctgaagca gaagaaattg tagcaacaaa agaagtaaat gtgttgcttt taggccctca
     1801 agttcgctat ttactagggg actttcaaga aaaactaaaa gatagacaga ttcctgtggc
     1861 ggttattccg atgacagatt atggaatgat gaatggttct aaagtcttag atttagctga
     1921 aagtttatta gactaagatt gaggagaatg ctatgaatga aagtaattta gaatctgtaa
     1981 tggggctaat tatgtatggt ggggaagcca aaagtaatgc tatggaggct attcaggcag
     2041 caaaaaaagg ggatttctct aaagccaatc gaagattagc tgatgcgaat gctgccttat
     2101 tacaggcgca taaggctcaa acagaaatgt tgacaagaga ggcacagggg gaagaaacat
     2161 caattagcct cttgatggta cacgcgcaag atcatttaat gacgagtctg acctttgttg
     2221 acttatcaaa agaagtggta gaagtgtaca aacgatttga aaaaaattag gaatttgcta
     2281 tgaatacaat gttggataaa atgcaagaaa aactttctcc aattgcaatg aaagttggga
     2341 atcagaagtt tttagttgct ttacgtgatt catttgtggg gactatgcct gttattatga
     2401 caggttccat tgctttgtta ttaaatgctt ttctagtgga tttaccacaa caatttcacc
     2461 tagaaagtat tacgaaaacc tttcagtggc ttgttgacat caataatttg gtattcaagg
     2521 gaagtatacc aattgtttcc ttgttgttca tctattgctt gggagtgaat atcgctaaga
     2581 tttacaaggt tgatacagtt tctgctggtt tggtatcact tgcttcgttt gtcatttcga
     2641 ttggaagtac agttacaaag agtttccctt tggcaaatgt aggagatgtg aaattagatc
     2701 agatcttaca aggaattgat aatctagcat ttgatggtaa aaatctgatg gtaactattg
     2761 ggaatgtgat tccaggaaac catattaatg ccagaggcta ctttacagcc atgatgattg
     2821 gatttttagc ttctattatt ttctgtaaag taatgaaaaa gaactgggta atcaagttac
     2881 cagattcagt tccccctgcg attgctaagc cgttcacttc tatcattcca ggatttatgg
     2941 cgatgtatat agtagccatc ttgacttatg ttttccattt acttagcaat gacttattga
     3001 ttgattgggt ttacaaggtg ttacaaacgc cattactagg tttgtctcaa agtttctttg
     3061 cagttattct gatgatcttt ttgaataagt tattctggtt ttttggtctc catggaggaa
     3121 atgtattggc tcccatcatg gaagggctat ttggagttgc tatgttagcg aatctggatg
     3181 ctttccaaaa aggtgaaccg attccttata tatggacaag tgggtctttt ggggcgtttg
     3241 tctggtttgg aggattagga ttagtacttg ctattttaat tttttcaaga aacagtcatt
     3301 atcgaaaagt tgctaaactt ggtctagcac cagttctgtt taatattggt gagccggtaa
     3361 attatggttt accagttgtc ttgaatcctt tgctatttat accatttgta cttagcccgg
     3421 ttttcatggc aacggtagct tactgggcaa caagctgggg tcttgtttca cctgttacac
     3481 aaaatgttac ttgggtaatg cctccaattt tatatggttt cttctctaca gcatttgatt
     3541 ggcgtgccat catcttatca gttgtttgtt tgattattag tgtcttgact tatttcccat
     3601 ttgtgaaaat ggcagataaa actgaattga gttaatggtc ttacttgcgt tttttgtgat
     3661 aaaatagaaa tagattacgt gtaagatgag ttaggtgtag gatttcttat ctccactaaa
     3721 attgttatta gacaaaggtt tatagtttat tgaagttgga atagtacact gtaacttcta
     3781 aaacagtact agaaaatgat ttgaatttcc taatcgattt agtcatattt tagttcattt
     3841 ttctataaaa cataacaaga atgttattgt tttatataga gagattgata tagaaagggg
     3901 tgcagtaaag gtagatatct tcttgtgcct cttttgccat attagaaagg agattttatg
     3961 gttcagacaa aacagccaaa tattattttg attgttgtag atcaaatgag agcagacgcc
     4021 ttatccttga atagtaagga taagctagtt agcactccaa cattagatat gatggctagt
     4081 gtaggttata attttgaaaa tgcctattct ccagttcctt cttgcgttcc tgctagggca
     4141 gctttattga ctggcttaga ccaagataaa agtggacgag ttggttatca agatgaggtg
     4201 ccttggaatt ttactaatac tcttccaaaa gtctttaaag atatgggtta tcaaacagag
     4261 tgtattggta agatgcatgt ttttccttcc agacagagac ttgggtttga tcatgtctta
     4321 cttcacgatg ggtatctaca tgttgataga aagtatgata aggcttatgg aagtcaattt
     4381 gattatgcta gtgactattt agccttttta aagggtaaag ttggttatga tgttgatttg
     4441 attgatgacg gaatggattg taattcttgg gaagctagac cttgggataa agatgagaag
     4501 ttacatccaa caaactgggt tgtgagtgag tccatttcat ttttacaaag aagagatcca
     4561 acggttcctt tttttctcaa aatgtctttt gaaaaaccac atgctccatt aaatccccca
     4621 aaatactatt ttgatatata tatggaacgc ttgcctcaat ttctcgattt gcatatcggg
     4681 aattgggaag tattagaaaa gcaaattccg tctatttatg ctttaagagg gaaattaaag
     4741 gaagatgatc aaagaagaat ggttgctgct tattttggat tgattactca tatagaccat
     4801 caaattagtc gctttttgac tgccctaaaa gaatttcgtc atgataaaga tacgattatc
     4861 tggtttgttt cggatcatgg agatcagctt ggtgaacatt atctgtttag aaagggatat
     4921 ccttatcagg gaagtatcca cattccttcg tttatttacg atccggctgg attgatagct
     4981 ggtaataggg gaacaatcaa gcaattagtg aaaattcaag atatttttcc ttcactcgtg
     5041 gatttggcag gaggaactac tacagatgaa ctagatggac ggagtgttaa gaatttatta
     5101 tttggacagt acgaggggtg gcgaacagaa tttcatggtg aacatgcttt aggaaaagat
     5161 tctagtcaat atattttgac agaccagtgg aaatttatct ggtttcctgt tttaaatcat
     5221 tatcagttat ttgatatgaa aaaagatccg tatgaaatga atgatttgta tccaagtgaa
     5281 aaatatcaac ctattgttcg tcagatgaag aaaaaattag tagactttct tagatataga
     5341 gaagaaggtt ttgttgtgga tgaagagtta gttcctgttg aactttctaa aataacgcca
     5401 actctaacga aaacggggga tagtcaatca tgaaaatgaa aaaaatcaag ggtgggacat
     5461 ttatgatggg aacaaattct gaagagggct ttttagatga ttttgaaggt cctcaagtcg
     5521 ctgttagtgt aaaggatttt tccattgcgg atactccagt tacgaatcaa gaatttgctc
     5581 aatttgtgaa agaaacaggc tataaaacac tagctgagcg acaagagtgg tcttttgttt
     5641 ttatattgtt cgttccggag gcagaaaggg agggatatcc tcatcctgct ggggctccgt
     5701 ggtggttaca agtttcgaac gcttgctgga aacatcccta cggtgagaac agtaatctag
     5761 tgggtttaga agaccatcca gtagttcatg ttgctttaga agatgcattg gctttctgta
     5821 attggagcgg aatgtcttta ccgactgaag cacagtggga atatgctgct agaggaggaa
     5881 gacaatctga atatccttgg ggagatactc ttttagaagg tggatattat catgccaata
     5941 cttggcaagg aagatttcct tatgaaaata ctgccttaga tggatttata ggaacggctc
     6001 ctgtctatga atttttacct aatgattttg gtctatatca aatgattggg aatgtttggg
     6061 aatggtgtcg caatccgaga tataccttat tagctagttt taatgaagat gactatgagt
     6121 taccaaaata tgggatacaa gatgaggagt atgctatcag aggaggttcc tttttatgtc
     6181 attgttctta ttgtaatcga taccgagtag ctgctagaaa tggatgtatt tctacttcca
     6241 ctagtagcca tctaggtttt agatgtttga aagaatagga tggacttaaa aagtggttga
     6301 gattatttat tttctaatca ttataatagc tagtggattg ggttctatat caggcatggg
     6361 tggtggaatt ataattaagc cacttatgga tagttttggg taccattcgg tcagtgatat
     6421 tgctttttat tctagtttct ctgtttttat aatggcgatt atttcaacaa tcaaaagatt
     6481 ttcacaaagt aaagagataa aatggagatt gatttttacg gtatcctttt cttctgtttt
     6541 aggaggattt ctaggtcatc ttatttttca agttttgtta tctcaattat cagtacggct
     6601 agtatccatt gttcaaatga ttttacta
//
LOCUS       DAVMEF010000107         6625 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMEF010000107 DAVMEF010000000
VERSION     DAVMEF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.107"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            19..1437
                     /locus_tag="VYQ30_001527"
     CDS             19..1437
                     /locus_tag="VYQ30_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1966608.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            1490..2197
                     /locus_tag="VYQ30_001528"
     CDS             1490..2197
                     /locus_tag="VYQ30_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966609.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            2292..3266
                     /gene="folP"
                     /locus_tag="VYQ30_001529"
     CDS             2292..3266
                     /gene="folP"
                     /locus_tag="VYQ30_001529"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1966610.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            3268..4590
                     /locus_tag="VYQ30_001530"
     CDS             3268..4590
                     /locus_tag="VYQ30_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1966611.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            4571..5125
                     /gene="folE"
                     /locus_tag="VYQ30_001531"
     CDS             4571..5125
                     /gene="folE"
                     /locus_tag="VYQ30_001531"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1966612.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            5168..5980
                     /gene="folK"
                     /locus_tag="VYQ30_001532"
     CDS             5168..5980
                     /gene="folK"
                     /locus_tag="VYQ30_001532"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1966613.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(6130..6447)
                     /locus_tag="VYQ30_001533"
     CDS             complement(6130..6447)
                     /locus_tag="VYQ30_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1966614.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT         1820 a         1261 c         1577 g         1967 t
ORIGIN      
        1 attaaaagga gattgatgat ggacaaatta tttaaactaa aagagaacgg tacagacgtt
       61 cgtacagagg ttctcgctgg tttaacaact ttctttgcaa tgagctatat tctctttgta
      121 aacccacaaa ttctttcgca aacaggaatg cctgctcagg gtgtgtttct tgcaacgatt
      181 atcggtgcag ttgctggtac cttgatgatg gccttctatg ctaacttgcc atacgctcaa
      241 gcaccaggta tgggactcaa tgccttcttt acctttacag tcgtatttgg acttggttat
      301 tcatggcaag aagccctagc tatggtcttc atctgtggga ttatttcatt ggttattacc
      361 ttgacaaatg ttcgtaaaat gatcattgaa tcgattccaa atgcccttcg ctcggctatt
      421 tcagctggta tcggtgtctt ccttgcctat gtagggatta agaatgctgg acttttgaaa
      481 tttacgattg atccaggcaa ctatactgtt gtaggagaag gggctgacaa agctcaagca
      541 acgattgcag caaactcttc agcagttcca ggattggtca gctttaataa tccagctgtt
      601 ttagtggctc ttgcaggact tgccattact atcttctttg tcatcaaagg gattaaaggg
      661 ggaattattc tctctatctt gacaacaact gttcttgcta ttgcagttgg tttggttgat
      721 ttgtctagta tcgattttgc taataaccat gttggtgcag cttttgaaga tttgaagaca
      781 atctttggtg cagctcttgg ttcagaagga ttgggagctt tggtttcaga tacagctcgc
      841 ttgcctgaaa ctctgatggc cattcttgcc ttctcattga cagatatttt tgacacaatt
      901 ggtaccttga tcggtacagg tgaaaaagtt ggtattgtag cgacaaatgg tgaaaatcac
      961 caatcagcca aattggataa ggctctttac tctgatttga ttggaacgac agtcggtgcc
     1021 attgcaggta cttcaaacgt aacgacttat gttgagtctg ctgctggtat cggtgcaggt
     1081 ggacgtactg gtttgacagc cttggttgta gctatctgtt ttgcgatttc aagcttcttt
     1141 agcccacttc tagcgatcgt accaacagcg gctacagctc caatcttgat tatcgttggg
     1201 attatgatgc ttggtagctt gaaaaatatc cattgggatg atatgtctga agcagttcct
     1261 gccttcttca catctatctt tatgggattc agctactcta tcactcaagg gattgcagtt
     1321 ggtttcttga cttacacttt gactaagctt gtcaaaggtc aagttaaaga tgttcatgcc
     1381 atgatttgga ttttggatgc cttgtttatc cttaactaca tcagcatggc cttataatag
     1441 aatgacccag ggggatttcc cccctttttt aatacaaagg agataggtga tgaaagagaa
     1501 aaatatgtgg aaagaattgt tgaatcgtgc aggctggatt ttggtctttt tacttgccgt
     1561 ccttttatat caggttcccc tagtggttac ctctattttg actttaaaag aagtagccct
     1621 gctacagtca gggctgatag ttgctggcct ttcaattgtg gttctggtgt tatttattat
     1681 tggagctcgt aaaacgcaat tggcaagttt taatttttct ttttttagaa ctaaggattt
     1741 ggcacgtttg ggcttgagtt atttagtcat tatctgttca aatatacttg gttctatctt
     1801 gttgcaactg tcaaatgaga cgacaacagc taaccagtct cagattaacg atatggttca
     1861 gaatagttct ctgatttcta gcttcttctt gctagccttg cttgctccaa tttgtgagga
     1921 aatcttgtgc cgtgggcttg ttcctaaaaa gattttccga ggcaaggaga acttgggatt
     1981 tgtagtcggt acgattgtgt ttgctttatt gcatcaacca agtaatttac cttctttatt
     2041 gatttatgga ggtatgtcga cagttctatc ttggacggcc tacaagaccc aacgtttgga
     2101 aatgtcgatc ttgcttcaca tgattgttaa tgggattgct ttctgtttgt tggctcttgt
     2161 ggtgattatg agtcggacat taggaatttc tgtgtaaaag tttttatgta ggaaccgacc
     2221 tctttctact agggaaagat gaatgcaatc gtgtccatct ttttcttttt atggtaaaat
     2281 agaaaaataa tatgatgaaa atccttgagg gagtgaccga tatgtcaagt aaagccaatc
     2341 atgcaaagac agttatttgc ggaattatca atgtaacccc agactccttt tcggacggtg
     2401 gtcaattttt tgctcttgag caggcactcc agcaggctcg taaattgata gcagaaggag
     2461 ccagtatgct cgatatcggc ggagaatcga ctcggccggg aagtagctat gttgagatag
     2521 aagaggaaat ccagcgtgtt gttccagtga tcaaagcgat tcgcaaggaa agtgatgtcc
     2581 tcatctctat tgatacttgg aagagtcaag tagcagaggc tgctttggct gctggtgccg
     2641 atctagtcaa tgatatcact ggtcttatgg gtgatgagaa aatggctcat gtggtagctg
     2701 aagcgagagc gcaagtggtc atcatgttta atccagttat ggcgcgacct cagcacccta
     2761 gctcgctcat cttccctcat tttggttttg gtcaagcttt tacagaggaa gagttagcgg
     2821 actttgaaac attgccaatc gaagaattga tggaggcttt ctttgaacga gcactagcga
     2881 gagcggcaga agctggtatt gcaccagaaa atatcctgtt ggatccagga attggctttg
     2941 gtctgaccaa gaaagaaaat ctgcttcttt tacgggacct ggataaacta catcagaagg
     3001 gctatccaat ctttctcgga gtgtcgcgca agcgatttgt catcaatatc ctagaggaga
     3061 atggttttga agtcaatcct gagacagagc ttggtttccg caatcgggac acggcttcgg
     3121 ctcatgtaac cagtatcgct gcaagacagg gtgtagaagt ggtgcgcgtg catgacgtag
     3181 ctagtcacag gatggcagtt gaaattgcct ctgccattcg tctggctgat gaagcggaaa
     3241 atttagattt aaaacaatat aaataagatg aaagaaattg aaaacaatca gtggattgct
     3301 aactaccgga cggatcaacc gcattttggc ttggaacgaa tggtggaact gttagctttg
     3361 cgtggcaatc cccatctcaa actcaaggtc ctccatatcg gaggaactaa cggcaagggc
     3421 tcgactattg cttttttgaa aaagatgcta gaaaagctag ggctgagagt tggcgtgttt
     3481 agctcgccct atctcattca ttacacagac cagattagca tcaatgggga atcgatctca
     3541 gaagcgaggc tagaagctct catggcagac tatcagtctt tgctggaggg agaagcggtc
     3601 gccaatttac agggcacaac cgagtttgag attatcacag ccctggccta tgactacttt
     3661 gcctcagagc aagtagatgt ggccatcatg gaagttggca tgggtggact ttttgatagt
     3721 accaatgtct gtcagcccat tttgacagga attacaacta ttggcttgga tcatgtggcc
     3781 ttacttggtg acactttgga ggccatagca gagcagaagg caggtattat caaacaaggg
     3841 atgcccttgg taacagggcg tattgctcca gaagccttgg ctgtgattga ccgcattgcg
     3901 gaagggaaag atgcgccgag acttgcctac gggacagatt atcaggttcg tcatcaagaa
     3961 agtgtggtga caggggaagt ctttgactat acaagtgctg tcagacaagg tcgcttccag
     4021 actagcctgc ttggtttgta ccaaatagag aatgctggga tggccatagc tttacttgat
     4081 actttttgtc aagaagatgg tcgagagcta gcaagcaatg actttcttgg tcaagccttg
     4141 gaagaaacaa gttggccagg gcgtttggaa atcgtgtcaa gagatccctt gatgattttg
     4201 gatggagccc ataatcccca tgctatcaag gccttgttgg taaccttgca agaacgtttt
     4261 gcggattatc ataaggaaat cctcttcact tgtatcaaaa ccaaagcctt ggaggatatg
     4321 ttggacttgc tgggagccat gccagatacc gagcttactc taacacattt tgcggatagt
     4381 cgggcgacgg atgaaagcgt gctgaaagag gcagctaagt ctagaaatct cagctaccaa
     4441 gattggcatg attttctaga gcagaatttg acagataaaa aagaagagaa acaaacagtt
     4501 aggattgtca caggttcctt gtatttcttg agccaagtga gggcctatct gatggagagg
     4561 aagaacgaga atggatacac aaaagattga agcagctgta aaaatgatta tcgaggctgt
     4621 aggagaggac gctaatcgcg agggcttgca ggaaacacct gctcgtgtag cccgtatgta
     4681 tcaagagatt ttttcaggtc ttggtcaaac agcagaggaa catttgtcaa aatcctttga
     4741 aattattgac gataatatgg tggtagaaaa ggatatcttt ttccatacca tgtgtgaaca
     4801 ccacttcttg ccattttatg gtagagcgca cattgcctac attccagatg gtcgtgtggc
     4861 aggcttgtct aagctagccc gtacggttga agtttattcg aaaaaaccac aaattcaaga
     4921 acgtttgaat atcgaagtgg ccgatgcctt gatggactat ctaggtgcta aaggagcctt
     4981 tgttgtcatt gaggcggaac atatgtgtat gagtatgcgt ggtgttagaa aaccaggcac
     5041 tgcaaccttg acgacagtag ctcgtggtct atttgaaaca gataaggacc tccgcgacca
     5101 agcttatcgt ttaatggggc tataaaaaga atccgtttca agcggatttt tctagaaagg
     5161 aatcattatg gatcaactgc agattaagga tttggaaatg tttgcctatc atggtctttt
     5221 tcctagtgag aaagaattgg ggcagaaatt tgtcgtttca gccatcctat cctatgatat
     5281 gaccaaggca gctacagact tggatttaac agcctctgtc cattacggag aattgtgtca
     5341 gcagtggacg acttggtttc aggaaacgag tgaagatttg attgaaacgg tagcctataa
     5401 actggtggaa cgtacctttg agtcttatcc tcttgtccaa gaaatgaagt tggaactgaa
     5461 aaaaccttgg gcaccggtgc atttgtcact agatacttgc tcggtaacca ttcatcgccg
     5521 caagcaacga gcctttatcg ccctaggaag caatatggga gataagcaag caaacttgaa
     5581 gcaagccatt gacaaactgc gagctcgtgg catccatatt ctcaaagagt ccagtgtctt
     5641 agcgacggag ccttggggtg gagtggagca ggatagcttt gccaatcaag tggttgaggt
     5701 ggaaacctgg ctaccagcac aagacttgtt agaaaccttg ttagccattg agtcagagct
     5761 gggacgggtg agagaagtgc attggggacc tcgtttgatt gatttggact tgctctttgt
     5821 ggaggaccag atcctttata cagacgacct catattgcct catccttaca tagcggaacg
     5881 cctttttgtc cttgagtcct tacaggaaat tgcgcctcat tttatccatc cgatattaaa
     5941 acaaccgatc cgcaacttgt atgatgcttt gaaaaaatag aaaaactcta gttttcagtc
     6001 acttgcaact gaaggctaga gtttttatac tcttcgaaaa tctcttcaaa ccacgtcagc
     6061 gtcgccttac cgtactcaag tacagcttgc ggctagcttc ctagtttgct ctttgatttt
     6121 cattgagtat taaaataggt cattttcttc tgggaggagg atagtttctc taccgtccat
     6181 gtctaaaacc agtactcttg ggggataacg agggtcgaaa ggatggttaa agtcaaaatc
     6241 aatggctgta gggaggtgtt gacttgaaaa gtggaaggta atctttcctt ggttattaag
     6301 caattgaaac tcgagttctt cttccaattc aaagacattt tttaagaaat ggtcgataat
     6361 ataccaaaaa gagtcaatga tgtcatcagg caagctggta acaatgccaa aactagcaga
     6421 ccgcatgtga gtattggtaa aagccatatc tctgtcccct ttcttttccc ttatcataca
     6481 gcaaatagga ttaaaaatca agaaaaggtg attttttgaa aaggatttta actacaggga
     6541 aaaagaagga aaaaaattcc caaaaatcta ccgaagttaa taggtaaatt cccaaattaa
     6601 cttgattata taactttcag ttact
//
LOCUS       DAVMEF010000108         6466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMEF010000108 DAVMEF010000000
VERSION     DAVMEF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.108"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(156..452)
                     /locus_tag="VYQ30_001534"
     CDS             complement(156..452)
                     /locus_tag="VYQ30_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1966615.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(442..603)
                     /locus_tag="VYQ30_001535"
     CDS             complement(442..603)
                     /locus_tag="VYQ30_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1966616.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMT
                     NPEGVEYGN"
     gene            673..1437
                     /locus_tag="VYQ30_001536"
     CDS             673..1437
                     /locus_tag="VYQ30_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1966617.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(1677..1829)
                     /locus_tag="VYQ30_001537"
     CDS             complement(1677..1829)
                     /locus_tag="VYQ30_001537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966618.1"
                     /translation="MRTKASIRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            2047..3426
                     /locus_tag="VYQ30_001538"
     CDS             2047..3426
                     /locus_tag="VYQ30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1966619.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3440..4105
                     /locus_tag="VYQ30_001539"
     CDS             3440..4105
                     /locus_tag="VYQ30_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1966620.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4415..4953
                     /locus_tag="VYQ30_001540"
                     /pseudo
     CDS             4415..4953
                     /locus_tag="VYQ30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            5020..5331
                     /locus_tag="VYQ30_001541"
     CDS             5020..5331
                     /locus_tag="VYQ30_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1966621.1"
                     /translation="MYDLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKTPIVDLYSAGKSRSLSGY
                     S"
     gene            5279..5431
                     /locus_tag="VYQ30_001542"
     CDS             5279..5431
                     /locus_tag="VYQ30_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1966622.1"
                     /translation="MWTYIQQENPEVFQAIRKTMLSRLTKHSVLPDRKLSNVVYQITK
                     SVYGFN"
     gene            5547..>6466
                     /gene="pavB"
                     /locus_tag="VYQ30_001543"
     CDS             5547..>6466
                     /gene="pavB"
                     /locus_tag="VYQ30_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1966623.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVK"
BASE COUNT         1752 a         1238 c         1491 g         1985 t
ORIGIN      
        1 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
       61 gcagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatt acttcatctt
      121 ctcctgaaat tgagtttttg cccagctcct cttgattatt ctacttgacc gggccaagca
      181 ttcataccac cttctacgtt ggtaacggtg aggccttggg agctgagaaa ttggcaggta
      241 gatgcagagc ggactcctcc ttgacaaata acatagtatt catggtcagg tttgagttct
      301 ttgtagcctt gctctaaggt acttaatgga agatttttgg cacctggtgc atgtcctgct
      361 tggaattcat gtgcttcacg gacatcgata agttctagat tttcattttg gtattttcca
      421 taaaagtcag ccatgctgat actagtttcc atattctact ccttctgggt tagtcatttt
      481 gtatagtgaa taagcgccgt caaggttttg ggcggtaaat cctgcttgct tgaggatagg
      541 ctctgcgata tagctgcgca aaccactgtg gcagctgacg atgtaggctt gttttttgtc
      601 cagttcatca gcttcctcct tgacactttg tcagtttttg atacaatagt acaaaattag
      661 aggaggcagg ctatgattca gaaacatgcg attcctattt tagagtttga tgacaatcct
      721 caggcggtta tcatgcccaa tcacgagggg ctggacttgc agttgccaaa gaagtgtgtt
      781 tatgcatttt taggtgagga gattgaccgc tatgcaaggg aagtaggggc ggattgtgtc
      841 ggcgaattcg tttctgctac caagacctat ccagtctatg tcatcaacta caagggtgag
      901 gaggtctgtc tggctcaggc tcctgttggc tccgctccag cagcccagtt tatggattgg
      961 ttgattggct atggtgtgga gcagattatc tctactggga cctgtggtgt gctagccgat
     1021 atagaggaaa atgcctttct agtccctgtt cgcgctctgc gagatgaggg agccagttac
     1081 cactatgtgg caccttgtcg ttatatggaa atgcagccag aggctattgc tgctattgag
     1141 gaagttttgg aagacagagg gattccttat gaagaagtca tgacctggac gacagacggt
     1201 ttttaccgag aaacggctga aaaggtggct tatcgtaagg aagaaggctg tgctgttgtg
     1261 gagatggagt gttctgctct tgcggcagta gctcaattgc gtggggttct ctggggtgaa
     1321 ttgttgttca cagcagattc tctagcggac ttggaccagt acgacagtcg tgactggggc
     1381 tcggaagctt ttaataaggc gctagaactg agtttagcaa gtgttcacca cctttagttg
     1441 tactggcaaa ggatttgttt tatcataaaa tgtctagctc atacttttca aaaatatgtt
     1501 taaacgaggt caccttcctc ttgtcctagg catgttgagg ttgggaaaaa tctttaaaat
     1561 cagaaaaacg tatcatatca ggtgatgaaa actttgatac tatgcgtttt atgttggtaa
     1621 gatttagagt gagatgaaat gatactcttc gaaaatcttt tcaaaccagg tcagcttcac
     1681 cttgccgtat agatgttact gacttcgtca gttctatctg caacctcaaa acagtgtttt
     1741 gagctgactt cgtcagttct atccacaacc tcaaaacagt gttttgagca acctgtgact
     1801 agctttctaa tcgatgcctt ggttctcatt gcctataatc aaaaagagaa attttctcct
     1861 gaaaagcata tagagtagct ggcgttaaaa gctcctgtct cgcttttttg acctatagtc
     1921 acatctatca agtattgttc ttgcctaagc tatcaataaa aaggtgacat tttttaggct
     1981 tggtgttagt agattttgcc ttatcctatc taagtcattt cgaacttttt atggtacaat
     2041 ggaaacatgt tattcaaatt atctaaggaa aaaatagagc taggcttatc tcgtttatcg
     2101 ccagcccgtc gtattttttt gagttttgcc ttggtcattt tactaggttc tcttcttttg
     2161 agcttgccct ttgtccaagt tgaaagctca cgagcgactt attttgatca tcttttcact
     2221 gctgtctctg cagtctgtgt gacaggtctc tcaacccttc cagtagctca cacctataat
     2281 atctggggcc aaataatctg tttgctcttg attcagatcg gtggtctagg gctcatgacc
     2341 tttattgggg ttttctatat ccagagcaag caaaagctta gtcttcgtag ccgtgcaact
     2401 attcaggata gttttagtta tggagaaact cgatctttga gaaagtttgt ctattctatt
     2461 tttctcacga cctttttggt tgagagctta ggagctattt tgcttagttt tcgccttatt
     2521 cctcaacttg gctggggacg tggtcttttt agttccattt ttctagcgat ctcagccttc
     2581 tgtaatgccg gttttgataa tttagggagc accagtttat ttgcttttca gaccgattta
     2641 ctggtcaatc tggtgattgc aggcttgatt attacaggcg gccttggttt tatggtctgg
     2701 tttgatttgg ctggtcatgt aggaagaaag aaaaaaggac gtctgcactt tcatacgaag
     2761 cttgtactat tgttgactat aggtttgttg ttatttggaa cggcaactac tctctttctt
     2821 gagtggaaca atgctggaac gattggcaat ctccctgttg ccgataaggt tttagttagc
     2881 ttttttcaaa cagtgacgat gcgaacagct ggcttttcta cgatagatta tactcaggct
     2941 catcctgtga ctcttttgat ttatatctta cagatgtttc taggtggggc acctggagga
     3001 acagctgggg gactcaagat tacgacattt tttgtcctct tggtctttgc acgaagtgag
     3061 cttctaggct tgcctcatgc caatgttgcg agacgaacga ttgcgccgcg aacggttcaa
     3121 aaatccttta gtgtctttat tatctttttg atgagcttct tgataggatt gattctgcta
     3181 gggataacag ccaaaggcaa tcctcccttt atccacctca tatttgaaac catttcagct
     3241 cttagtacag ttggtgtaac ggcaaatctg actcctgacc ttgggaaatt ggctctcagt
     3301 gttatcatgc cacttatgtt tatgggacga attggtccct tgaccttgtt tgttagcttg
     3361 gcagattacc atccagaaaa gaaagatatg attcactata tgaaagcaga tattagtatt
     3421 ggttaagaaa ggaaagagca tgtcagatcg tacgattgga attttgggct tgggaatttt
     3481 tgggagcagt gtcctagctg ccctagccaa gcaggatatg aatattatcg ctattgatga
     3541 ccacgcagag cgcatcaatc agtttgagcc agttttggcg cgtggagtga ttggtgacat
     3601 cacagatgaa gaattattga gatcagcagg gattgatacc tgcgataccg ttgtagtcgc
     3661 gacaggtgaa aatctggagt cgagtgtgct tgcggttatg cactgtaaga gtttgggggt
     3721 accgactgtt attgctaagg tcaaaagtca gaccgctaag aaagtgctag aaaagattgg
     3781 agctgactcg gttatctcgc cagagtatga aatggggcag tctctagcac agaccattct
     3841 tttccataat agtgttgatg tctttcagtt ggataaaaat gtgtctatcg tggagatgaa
     3901 aattcctcag tcttgggcag gtcaaagtct gagtaaatta gacctccgtg gcaaatacaa
     3961 tctgaatatt ttgggtttcc gagagcagga aaattcccca ttggatgttg aatttggacc
     4021 agatgacctc ttgaaagcag atacctatat tttggcagtc atcaacaacc agtatttgga
     4081 taccctagta gcattgaatt cgtaaagagg gatgacccct cttttttgat gcccaagatg
     4141 gcaaatagag acagaagccc cttgtcttct agtaaaagtt cttcaaaggc tggactttat
     4201 ggtaaaatag aaggaagtga caagagagag taatactcaa tgaaaatcaa agatcaaact
     4261 aggaaactag ctgcgggctg ctcaaaacac tgttttgagg ttgcagatag aactgacgaa
     4321 gtcagtaaca tctatacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt
     4381 ataagaaaaa atcagtcccc taaaggagca gattatgaag ttattgtcta tcgctatttc
     4441 tagctataat gcagcagcct atcttcatta ctgtgtggag tcgctagtga ttggtggtga
     4501 gcaagttggg attttgatta tcaatgacgg gtctcaggac cagactcagg aaatcgctga
     4561 gtgtttagct agcaagtatc ctaatatcgt tagagccatc tatcaggaaa ataaaggcca
     4621 tggcggtgtg gtcaatcgcg gcttggcaga ggcttctggg cgctatttta aagtagttga
     4681 cagtgatgac tggtggatcc tcgtgcctac ttgaaaattc ttgaaacctt gcaggaactt
     4741 gagagcaagg gtcaagagat ggatgtcttt gtgaccaatt ttgtctatga aaaggaaggt
     4801 cagtctcgta agaagagtat gagttacgag tcagtcttgc ctgttcggca gatttttggc
     4861 tgggaccagg tcggaaattt ctccaaaggc cagtatatca tgatgcactc gctgatttat
     4921 cggacagatt tgttgcgtgc tagccagttc taactgcctg aacatacttt ttatgtcgat
     4981 aatctctttg tctttacgcc ccttcagcag gtcaagacca tgtacgatct gcctgtcgat
     5041 ttctatcgtt acttgattgg gcgtgaggac cagtctgtca atgagcaagt gatgattaag
     5101 tgcattgacc agcaactcaa ggtcaatcga ctcttggtag accagcttga tttgtcccaa
     5161 gtgagtcatc ccaaaatgcg agaatatctg ctgaatcata ttgaaatcac gacggtgatt
     5221 tccagtaccc tgctcaaccg atctgaaaca gcggagcatc tggcaaaaaa aacgccaatt
     5281 gtggacctat attcagcagg aaaatccaga agtctttcag gctattcgta agaccatgtt
     5341 gagccgtttg accaaacatt ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca
     5401 aatcaccaaa tctgtttatg gatttaatta atataagtgt tttataagag ggatttaaga
     5461 aaaattttaa ctttttctta gtccttttta atttcaggag attatactag agtcatcaaa
     5521 taaagaaaga ctctaaggag aatcctatga aattcaatcc aaatcaaaga tatactcgtt
     5581 ggtctattcg ccgtctcagt gtcggtgttg cctcagttgt tgtggctagt ggcttctttg
     5641 tcctagttgg tcagccaagt tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag
     5701 tcttacctga agagacatcg ggaacgaaag agggtgactt atcagaaaaa ccaggagaca
     5761 ccgttctcac tcaagcgaaa cctgagggcg ttactggaaa tacgaattca cttccgacac
     5821 ctacagaaag aactgaagtg agcgaggaaa caaactcttc tagtctggat acactttttg
     5881 aaaaagatga agaagctcaa aaaaatccag agctaacaga tgtcttaaaa gaaactgtag
     5941 atacagctga tgtggatggg acacaagcaa gtccagcaga aactactcct gaacaagtaa
     6001 aaggtggagt gaaagaaaat acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa
     6061 aagctgaagg gaaaggtcct ttcactgccg gtgtaaacca agtaattcct tatgaactat
     6121 tcgctggtga tggtatgtta actcgtctat tactaaaagc ttcggataat gctccttggt
     6181 ctgacaatgg tactgctaaa aatcctgctt tacctcctct tgaaggatta acaaaaggga
     6241 aatacttcta tgaagtagac ttaaatggca atactgttgg taaacaaggt caagctttaa
     6301 ttgatcaact tcgcgctaat ggtactcaaa cttataaagc tactgttaaa gtttacggaa
     6361 ataaagacgg taaagctgac ttgactaatc tagttgctac taaaaatgta gacatcaaca
     6421 tcaatggatt agttgctaaa gaaactgtcg aaaaagctgt taaaga
//
LOCUS       DAVMEF010000109         6466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMEF010000109 DAVMEF010000000
VERSION     DAVMEF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.109"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            13..6150
                     /locus_tag="VYQ30_001544"
     CDS             13..6150
                     /locus_tag="VYQ30_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1966624.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVTDKGEPAVQPELPEAVVTDKD
                     EPEVQPESPDTVVSDKGEPEQVAPLPEYTGPQAGAIVEPAVQPALPEAVVSDKGEPEQ
                     VAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQV
                     AENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDSTTSVGESNQPENNDSKNENSE
                     KTREEVPVNPNEGTVEGTSNQETENPVQPAEESTTNSEKVSPDTSSENTGEVSNKPSD
                     SKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGNSTEDVSTKSNTSNSNGN
                     EEIKQENELDPDKKVEDPEKTLELRNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFV
                     KVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEIESRYKDNFTFYLAKKGT
                     EETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLIGEKD
                     GKHYAIYNLKKPLFESLRGATIEKLSLKNVSISGKDDIGSLANEAQNNTKIKQVHVDG
                     VLAGERGIGGLLAKADQSSITESSFKGRIVNTYETTASYNIGGLVGHLTGSKASLTKS
                     KATVVISSNTNSSDQTVGGIAGLVDKDAHIQNSYSEGDINNSQRFGKVAGIAGNLWDR
                     ESNSENHAGRLTNVLSDVNVTNGNAISGYHYNGMKITDAFSNKANKVFNVTLEKDEVV
                     SKESFEERGTMLEASQIASKKAEINLLTPPIVEPLSTSGKKDSDFSKIAHYQANRALV
                     YKNIEKLLPFYNKATIVKYGNLVKENSILYQKELLSAVMMKDDQVITDIISNKQTANK
                     LLLHYKDHSSEKFDLRYQDDFANLAEYSIGDSGLLYTPNQFLYDQDSIINQVLPELQQ
                     VAYDSEAIRKTLGISPEVKQTELYMEDQFTKTKQDLANSLKKLLSADAGLAGDNPVTR
                     GYLVDKIKNNKEALLLGLTYLERWYNFSYGQVNVKDLVMYHPDFFGKGNTSPLDTLIE
                     LGKSGFNNLLAKNNVDTYAISLASHHGTTDLFSTLENYRKVFLPDKTNNNWFKSQTKA
                     YIVEEKSNIEEVKTKQGLVGTKYSIGVYDRITSATWKYRNMVLPLLTLPEKSVFVIST
                     ISSLGFGAYDRYRNSEHKADKDLNDFVEENARETAKRQRDHYDYWYRILDDNAREKLY
                     RNILLYDAYKFGDDNTVGKATKVASFDDPNPAMKYFFGPVGNKVGHNGHGAYATGDAV
                     YYMGYRMLDKDGAITYTHEMTHNSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDQPSDA
                     TITINSILKHSKSDSKEGERLQVLDPTTRFKDATDLQKYVHNMFDVVYMLEYLEGQSI
                     VKKLNVYQKIEALRKIENKYVKDREDGNKVYATNVVQNLTEEDANKLTTFDSLIKNNI
                     LSAREYQSGDYERNGYYTIKLFAPIYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGM
                     VPYISNQYEEDAKQQGQTINLYGKERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERV
                     DQFGNLKQVTFKDPTKPWPSYGTKTINNVDELQALMDQAVLKDAEGPRWSNYDPEIDS
                     AVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6202..6378
                     /locus_tag="VYQ30_001545"
     CDS             6202..6378
                     /locus_tag="VYQ30_001545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1966625.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
BASE COUNT         2360 a         1136 c         1307 g         1663 t
ORIGIN      
        1 caaggaggaa taatggaaaa gtattttggt gaaaaacaag agcgtttttc atttagaaaa
       61 ttatcagtag gacttgtatc tgcaacgatt tcaagtttat tttttatgtc tgtattagct
      121 agttcatctg tggatgctca agaaactgcg ggagttcact ataaatatgt ggcagattca
      181 gagctatcat cagaagaaaa gaagcagctt gtctatgata ttccgacata cgtggagaat
      241 gatgatgaaa cttattatct tgtttataag ttaaattctc aaaatcaact ggcggaatta
      301 ccaaatactg gaagcaagaa tgagaggcaa gccctagttg ctggtgctag cttagctgct
      361 ctgggaattt taatttttgc tgtttccaag aaaaaggtta agaataaaac ggtattacat
      421 ttagtattgg ttgcgggaat gggaaatggt gtcttagttt cagtccatgc tttagaaaat
      481 catcttttgc taaattacaa tacggactat gaattgacct ctggagaaaa attacctctt
      541 cctaaagaga tttcaggtta cacttatatt ggatatatca aagagggaaa aacgacttct
      601 gattttgaag taagtaatca agaaaaatca gcagccactc ctacaaaaca acaaaaggtg
      661 gattataatg ttacaccaaa ttttgtagac catccatcaa cagtacaagc tattcaggaa
      721 caaacacctg tttcttcaac taagccgaca gaagttcaag tagttgaaaa acctttctct
      781 actgaattaa tcaatccaag aaaagaagag aaacaatctt cagattctca agaacaatta
      841 gccgaacata agaatctaga aacgaagaaa gaggagaaga tttctccaaa agaaaagact
      901 ggggtaaata cattaaatcc acaggatgaa gttttatcag gtcaattgaa caaacctgaa
      961 ctcttatatc gtgaggaaac tatagagaca aaaatagatt ttcaagaaga aattcaagaa
     1021 aatcctgatt tagctgaagg aactgtaaga gtaaaacaag aaggtaaatt aggtaagaaa
     1081 gttgaaatcg tcagaatatt ctctgtaaac aaggaagaag tttcgcgaga aattgtttca
     1141 acttcaacga ctgcgcctat tccaagaata gtcgaaaaag gtactaaaaa aactcaagtt
     1201 ataaaggaac aacctgagac tggtgtagaa cataaggacg tacagtctgg agctattgtt
     1261 gaacccgcaa ttcagcctga gttgccagaa gcagtcgtaa ccgacaaagg tgagcccgct
     1321 gtccaacctg aattacccga agcagtcgta accgacaaag acgagcctga agttcaacca
     1381 gagtcgccag atactgtggt aagtgataaa ggtgaacctg aacaggtagc accgcttcca
     1441 gaatatacag gaccacaagc cggagctatt gttgaacccg cagtccagcc agcgttgccc
     1501 gaagctgttg taagtgataa aggtgaacca gagcaggtag ccccattgcc agaatataag
     1561 gggaatattg agcaagtaaa acctgaaact ccggttgaga agaccaaaga acaaggtcca
     1621 gaaaaaactg aagaagttcc agtaaaacca acagaagaaa caccagtaaa tccaaatgaa
     1681 ggtactacag aaggaacctc aattcaagta gcagaaaatc cagttcaacc tgcagaagaa
     1741 tcaacaacga attcagagaa agtatcacca gatacatcta gcgaaaatac tggggaagtg
     1801 tccagtaatc ctagtgattc gacaacctca gttggagaat caaatcaacc agaaaataat
     1861 gactctaaaa atgaaaattc agaaaaaact agagaagaag ttccagtaaa tccaaatgaa
     1921 ggcacagtag aaggtacctc aaatcaagaa acagaaaatc cagttcaacc tgcagaagaa
     1981 tcaacaacga attcagagaa agtatcacca gatacatcta gcgaaaatac tggggaagta
     2041 tccaataaac ctagtgattc aaaaccacca gttgaagaat caaatcaacc agaaaaaaac
     2101 ggaactgcaa caaaaccaga aaattcaggt aatacaacat cagagaatgg acaaacagaa
     2161 ccagaaccat caaacggaaa ttcaactgag gatgtttcaa ccaaatcaaa cacatccaat
     2221 tcaaatggaa acgaagaaat taaacaagaa aatgaactag accccgataa aaaggtagaa
     2281 gatccagaga aaacacttga attaagaaat gtttccgacc tagagttata cagtttgtca
     2341 aatggtactt ataaacaaca catttcgtta gaacaagttc caagcaatcc aaatagctac
     2401 tttgttaaag tgaaatcttc ttcattcaaa gatgtatacc taccagtagc atcaatatca
     2461 gaggaaagaa aaaatgataa aatcctttat aaaatcacag caaaagtaga gaagcttcag
     2521 caggagatag aaagcagata taaagataat tttaccttct atctagctaa gaagggaaca
     2581 gaagaaacaa caaactttac ttcctttagt aatctggtca aagctataaa ccaaaatctc
     2641 tctggaacct atcatttagg agctagcctg aatgctaacg aagtggagct tagcactgat
     2701 gataaatcct atatcaaggg aacctttact ggtcagttga ttggtgaaaa agatggtaag
     2761 cattatgcta tttacaactt gaaaaagcct ctttttgaaa gcttgagagg ggccacaata
     2821 gaaaagttga gtctgaaaaa tgtctctatt tcagggaaag atgatatcgg ttcactggca
     2881 aatgaagctc agaataacac aaaaattaag caagttcatg tggatggtgt acttgccggt
     2941 gaacgtggta tcggtggttt gttggctaag gcagatcaat caagcatcac agagagtagt
     3001 ttcaagggaa gaattgtcaa tacctatgaa acaactgctt cctataatat cggtggcctg
     3061 gtcggtcatt tgacaggaag caaagcttca ctgactaagt caaaagcgac agtggtcatt
     3121 tcatcaaata caaatagttc tgaccagacc gttggaggta tcgctggtct tgtagacaaa
     3181 gatgcgcata ttcagaatag ctattctgaa ggggatatta ataatagcca acgttttgga
     3241 aaagttgctg gtatagcagg taacttatgg gatcgtgaat caaatagtga aaatcatgct
     3301 ggaagattga ctaatgtgct tagtgatgtc aatgtaacta atggaaatgc cattagtggt
     3361 taccattata atggaatgaa aattactgat gcatttagta ataaagcgaa taaagttttc
     3421 aatgtcactt tagagaagga tgaggtcgtt agcaaagaat cctttgagga aagaggaaca
     3481 atgctagagg cttctcaaat tgcaagcaaa aaagcagaaa tcaatcttct cactccacca
     3541 atagtggaac ccctctcaac aagcggtaag aaagacagtg atttttctaa gatagcccat
     3601 tatcaagcca accgtgcttt ggtttataag aacattgaaa aattgttacc tttctataac
     3661 aaggcgacca tcgtgaaata cggaaacctg gttaaggaga acagtatctt atatcaaaaa
     3721 gaactcttgt ctgcagttat gatgaaggat gaccaagtaa tcacagatat tatttccaac
     3781 aaacagactg caaacaaact cttgcttcac tacaaggacc attcatctga aaagtttgat
     3841 ctcaggtacc aggatgattt tgccaatttg gcagaatata gcattggaga tagtgggctt
     3901 ctctatacgc caaatcaatt cttgtatgac caagactcta tcatcaatca agttttacct
     3961 gaattacagc aggttgccta cgattcagaa gctatcagaa agacactcgg aatttctcca
     4021 gaggtcaagc aaactgagct ctatatggaa gaccagttta ccaaaacaaa gcaagatttg
     4081 gcaaacagtt tgaaaaaact cttgtctgca gatgctggac ttgctggtga caacccagtt
     4141 accagaggct atcttgtaga taaaatcaag aacaataagg aagccttact actcggttta
     4201 acttatttag aacgttggta taactttagc tatggtcagg tgaatgtcaa agacctagtt
     4261 atgtatcatc cggacttctt tggtaaagga aatacttcac cattagatac tctgattgag
     4321 ttaggtaaat ctggctttaa caatcttctt gctaaaaaca atgtcgatac ttatgctatc
     4381 agccttgcca gccatcatgg aacgacagat ttgtttagca cgttggaaaa ttaccgaaaa
     4441 gtctttctac cagacaaaac caataataac tggtttaaat cacagactaa ggcttacatt
     4501 gtcgaagaaa aatccaatat cgaagaggtg aaaacgaagc aaggactagt tggcactaag
     4561 tattctattg gtgtctatga ccgtatcact agtgccacat ggaaataccg caatatggtc
     4621 ttgccgctcc taaccttgcc ggagaaatca gtctttgtca tctcgaccat ctctagtcta
     4681 ggatttggag cttatgatcg ttaccgcaat agtgagcaca aagcagacaa ggacctcaat
     4741 gattttgttg aagaaaatgc acgtgaaaca gccaaacgtc agcgagatca ctacgattat
     4801 tggtatcgta ttttagacga caatgcacgt gaaaaacttt atagaaatat tttgctttac
     4861 gatgcttata aatttggcga tgataatacc gtagggaaag ctacaaaagt agcaagtttt
     4921 gatgatccaa atcctgcgat gaaatatttc ttcggtcctg ttggaaataa agttggtcat
     4981 aatggacatg gtgcttatgc tacaggggat gcagtatatt atatggggta tcgcatgcta
     5041 gataaggatg gagctattac gtatactcat gagatgacgc ataattcaga tcaagatatt
     5101 tatcttggtg gttatggaag aagaagtgga cttggacctg agttctttgc taaaggctta
     5161 ctacaagcac cagaccaacc aagtgatgca acgattacta tcaattctat attgaaacat
     5221 tcaaaatctg atagtaaaga aggagaacga ttacaagtac ttgatccaac tacgagattt
     5281 aaagacgcaa cagatcttca aaaatacgtc cacaatatgt ttgatgtcgt ttacatgttg
     5341 gaatatctcg aagggcaatc tattgttaag aaattgaatg tatatcagaa aatagaagct
     5401 ctcagaaaaa ttgagaacaa gtatgtgaaa gatcgtgaag atggaaataa ggtttacgct
     5461 actaacgttg tacaaaatct gacagaagaa gatgccaaca aattgactac ctttgatagt
     5521 ttaattaaaa ataatatctt atcagctcgt gagtaccagt ctggcgacta tgaacgaaat
     5581 ggctactata ccatcaaact ctttgcccca atctattcag ctctcagcag tgagaaaggc
     5641 acaccagggg accttatggg acgtaggatt gcgtacgaac ttttggctgc caaaggcttt
     5701 aaggatggaa tggtacctta tatctcaaac caatacgaag aagatgctaa acaacaaggg
     5761 caaactatca atctttatgg taaagaacgg ggcttggtga ccgatgagct tgttttgaaa
     5821 aaggtatttg acggtaagta taaaacttgg gcagaattta agacagctat gtaccaagaa
     5881 cgggtggatc agtttggaaa cttgaagcag gtgaccttta aagatccgac aaaaccatgg
     5941 ccaagctatg gcacaaagac tatcaataat gtggatgaat tgcaagccct catggaccaa
     6001 gctgttctca aggatgctga aggtccacgt tggagtaatt atgatccaga aatcgacagt
     6061 gccgttcata agttgaagag agcaatcttt aaagcctatc ttgaccaaac aaatgatttt
     6121 agaagttcaa tttttgagaa taaaaaatag tgtttactat taggaaataa agtttaaaaa
     6181 ggtgatgaag aacaaaccaa gattcaagca ggaattccta ctgataatga agtaagttat
     6241 gctcttattt atcagcagga aactcttcct gcaacaggtt catcaacttc tgagcttaca
     6301 gctttaggcc tattagctgt tggtagttta gttcttttgg ttcataatat gacgggaaca
     6361 gttttttgct ccctctgaaa agtcatcatt tgatggcttt tttctatata gggtaaaaga
     6421 taggctaaaa gatagggtaa aaggctatca tcggacaaaa taaaga
//
LOCUS       DAVMEF010000110         6396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMEF010000110 DAVMEF010000000
VERSION     DAVMEF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.110"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            52..4992
                     /locus_tag="VYQ30_001546"
     CDS             52..4992
                     /locus_tag="VYQ30_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1966626.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAKAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTDKDEASPKTILGIEVSQEP
                     KKDYLVGDSLNLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFLN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQLASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5082..6278
                     /locus_tag="VYQ30_001547"
     CDS             5082..6278
                     /locus_tag="VYQ30_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1966627.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
BASE COUNT         2112 a         1195 c         1438 g         1651 t
ORIGIN      
        1 atataaagcg ctttcttgaa aacaacaaaa tcaatctttt aggaggagaa aatgaagaat
       61 ccattttttg aaagacgttg tcgttacagt attcgtaagt tatcagtagg agcctgctcg
      121 ctgatgattg gtgctgtttt atttgctggt ccagccttgg ctgaagaaac tgcagttcct
      181 gaaaatagcg gagctaatac agagcttgtt tcaggagaga gtgagcattc gaccaatgaa
      241 gctgataagc agaatgaagg ggaacatact agagaaaaca agctagaaaa ggcagaagga
      301 gtagcgacag catctgaaac tgcagaagca gctagcgcag ctaaaccaga ggaaaaagca
      361 agtgaggtgg ttgcagaaac accatctgca aaagcaaaac ctaagtctga caaggaaaca
      421 gaagcaaagc ccgaagcaac taaccaaggg gatgagtcta agccagcagc cgaagctaat
      481 aagactgaaa aagaagtcca gccagatgtc cctaaaaata cagaaaaaac attaaaacca
      541 aaggaaatca aatttaattc ttgggaagaa ttgttaaaat gggaaccagg tgctcgtgaa
      601 gatgatgcta ttaaccgcgg atctgttgtc ctcgcttcac gtcggacagg tcatttagtc
      661 aatgaaaaag ctagcaagga agcaaaagtt caagccttat caaacaccaa ttctaaagca
      721 aaagaccatg cttctgttgg tggagaagag ttcaaggcct atgcttttga ctattggcaa
      781 tatctagatt caatggtctt ctgggaaggt ctcgtaccaa ctcctgacgt tattgatgca
      841 ggtcaccgta acggggttcc tgtatacggt acactcttct tcaactggtc taatagtatt
      901 gcagatcaag aaagatttgc tgaagctttg aagcaagacg cagatggtag cttcccaatt
      961 gcccgtaaat tggtagacat ggcaaagtat tatggctatg atggctattt catcaaccaa
     1021 gaaacaactg gagatttggt taaacctctt ggagaaaaga tgcgccagtt tatgctctat
     1081 agcaaggaat atgctgctaa ggtaaaccat ccaatcaagt attcttggta tgatgccatg
     1141 acctataact atggacgtta ccatcaagat ggtttgggag aatacaacta ccaattcatg
     1201 caaccagaag gagataaggt tccggcagat aacttctttg ctaactttaa ctgggataag
     1261 gctaaaaatg attacactat tgcaactgcc aactggattg gtcgtaatcc ttatgatgta
     1321 tttgcaggtt tggaattgca acagggtggt tcctacaaga caaaggttaa gtggaatgac
     1381 attttagacg aaaatgggaa attgcgcctt tctcttggtt tatttgcccc agataccatt
     1441 acaagtttag gaaaaactgg tgaagattat cataaaaatg aagatatctt ctttacaggt
     1501 tatcaaggag accccactgg ccaaaaacca ggtgacaaag attggtatgg tattgctaac
     1561 ctagttgcgg accgtacgcc agcggtaggt aatactttta ctacttcttt taatacaggt
     1621 catggtaaaa aatggttcgt agatggtaag gtttctaagg attctgagtg gaattatcgt
     1681 tcagtatcag gtgttcttcc aacatggcgc tggtggcaga cttcaacagg ggaaaaactt
     1741 cgtgcagaat atgattttac agatgcctat aatggcggaa attcccttaa attctctggt
     1801 gatgtagctg gtaagacaga tcaggatgtg agactttatt ctactaagtt agaagtaact
     1861 gagaagacca aacttcgtgt tgcccacaag ggaggaaaag gttctaaagt ttatatggca
     1921 ttctctacaa ctccagacta caaattcgat gatgcagatg catggaaaga gctaaccctt
     1981 tctgacaact ggacaaatga agaatttgat ctcagctcac tagcgggtaa aaccatctat
     2041 gcagtcaaac tatttttcga gcatgaaggt gctgtaaaag attatcagtt caacctcgga
     2101 caattaacta tctcggacaa tcaccaagag ccacaatcgc cgacaagctt ttctgtagtg
     2161 aaacaatctc ttaaaaatgc ccaagaagcg gaagcagttg tgcaatttaa aggcaacaag
     2221 gatgcagatt tctatgaagt ttatgaaaaa gatggagaca gctggaaatt actaactggc
     2281 tcatcttcta caactattta tctaccaaaa gttagccgct cagcaagtgc tcagggtaca
     2341 actcaagaac tgaaggttgt agcagtcggt aaaaatggag ttcgttcaga agctgcaacc
     2401 acaacctttg attggggtat gactgtaaaa gataccagcc taccaaaacc actagctgaa
     2461 aatatcgttc caggtgcaac agttattgat agtactttcc ctaagactga aggtggagaa
     2521 ggtattgaag gtatgttgaa cggtaccatt actagcttgt cagataaatg gtcttcagct
     2581 cagttgagtg gtagtgtgga tattcgtttg accaagccac gtaccgttgt tagatgggtc
     2641 atggatcatg caggggctgg tggtgagtct gttaacgatg gcttgatgaa caccaaagac
     2701 tttgaccttt attataaaga tgcagatggt gagtggaagc tagctaagga agtccgtggt
     2761 aacaaagcac acgtgacaga tatcactctt gataaaccaa tcactgctca agactggcgc
     2821 ttgaatgttg tcacttctga caatggaact ccatggaagg ctattcgtat ctataactgg
     2881 aaaatgtatg aaaagcttga tactgagagt gtcaatattc cgatggccaa ggctgcagcc
     2941 cgttctctag gcaataacaa ggtacaagtt ggctttgcag atgtaccggc tggagcaact
     3001 attaccgttt atgataatcc aaattctcaa actccgctcg caaccttgaa gagcgaagtt
     3061 ggaggagacc tagcaagtgc accattggat ttgacaaatc aatctggtct tctttattat
     3121 cgtacccagt tgccaggcaa ggaaattagt aatgtcctag cagtttccgt tccaaaagat
     3181 gacagaagaa tcaagtcagt cagcctagaa acaggaccta agaaaacaag ctacgccgaa
     3241 ggggaggatt tggaccttag aggtggtgtt cttcgagttc agtatgaagg aggaactgag
     3301 gacgaactca ttcgcctaac tcacgcaggt gtatcagtat caggttttga tacgcatcat
     3361 aagggagaac agaatcttac tctccaatat ttgggacaac cagtaaatgc taatttgtca
     3421 gtgactgtca ctgacaaaga tgaagcaagt ccgaaaacta ttttgggaat tgaagtaagt
     3481 caggaaccga aaaaagatta cctagttggt gatagcttaa acttgtctga aggacgcttt
     3541 gcagtggctt atagcaatga caccatggaa gaacattcct ttactgatga gggagttgaa
     3601 atttctggtt acgatgctca aaagactggt cgtcaaacct tgacgcttcg ttaccaaggt
     3661 catgaagtca actttgatgt tttggtatct ccaaaagcag cattgaacga tgagtacctc
     3721 aaacaaaaat tagcagaggt tgaagctgct aagaacaagg tggtctataa ctttgcttca
     3781 ccagaagtaa aagaagcttt cttgaaagca attgaagcgg ccgaacaagt gttgaaagac
     3841 catgaaacta gcacccaaga tcaagtcaat gaccgactta ataaattgac agaagctcat
     3901 aaagctctga atggtcaaga gaaatttaag gaagaaaaga cagaacttga tcgcttaaca
     3961 ggtgaggttc aagaactctt ggctgccaaa ccaaaccatc cttcaggttc tgccctagct
     4021 ccgcttcttg agaaaaacaa ggccttggtt gaaaaagtag atttgagtcc agaagagctt
     4081 gcaacagcga aacagagtct aaaagatctg gttgctttat tgaaagaaga caagccagca
     4141 gtcttttctg atagtaaaac aggtgttgaa gtacacttct taaataaaga gaagactgtc
     4201 atcaagggtt tgaaagtaga gcgtgttcaa gcaagtgctg aagagaagaa atactttgct
     4261 ggagaagatg cccatgtctt tgaaatagaa ggtttggatg aaaaaggtca agatgttgat
     4321 ctctcttacg cttctattgt gaaaatccca attgaaaaag ataagaaagt taagaaagta
     4381 tttttcttac ctgaaggcaa agaggcagta gaattggctt ttgaacaaac ggatagtcat
     4441 gttattttta cagcaccaca ctttactcat tatgcctttg tttatgaatc tgctgaaaaa
     4501 ccacaacctg ctaaaccagc accacaaaac aaagtccttc caaaacctac ttatcaactg
     4561 gcttctgatc aacaaaaggc tcctaaattg gaagttcaag aggaaaaggt tgcctttcat
     4621 cgtcaagagc atgaaaatgc tgagatgcta gttggggaac aacgagtcat tatacaggga
     4681 cgagatggac tgttaagaca tgtctttgaa gttgatgaaa acggtcagcg tcgtcttcgt
     4741 tcaacagaag tcatccaaga agcgattcca gaaattgttg aaattggaac aaaagtaaaa
     4801 acagtaccag cagtagtagc tacacaggaa aaaccagctc aaaatacagc agttaaatca
     4861 gaagaagcaa gcaaacaatt gccaaataca ggaacagctg atgctaatga agccctaata
     4921 gcaggcttag ccagcctcgg tcttgctagt ttagccttga ccttgagacg gaaaagagaa
     4981 gataaagatt aaatatcgaa aaatcttgtg aaatctttcc ttatatttcc aaagtgtgat
     5041 ataatagttt cgaataaaat aaataaagga gtttttgtaa catggcaaaa cttactgtta
     5101 aagacgttga cttgaaaggt aaaaaagtcc tcgttcgtgt tgacttcaac gtaccattga
     5161 aagatggcgt aatcactaac gataaccgta tcacagcagc tcttccaact attaagtaca
     5221 tcatcgaaca aggtggacgt gcaattcttt tctctcacct tggacgtgtg aaagaagaag
     5281 ctgataaagc tggtaaatca cttgctcctg tagcagcaga cttggcagca aaacttggtc
     5341 aagatgttgt tttcccaggt gtcactcgtg gtgctgaatt ggaagcggca atcaacgctc
     5401 ttgaagatgg acaagttctc ttggttgaaa acactcgtta cgaagatgtt gacggcaaga
     5461 aagaatctaa aaacgatcct gaacttggta aatactgggc atcacttgga gatggtatct
     5521 tcgtaaacga tgcattcggt acagctcacc gtgcacacgc atctaacgtt ggtatctcag
     5581 caaacgttga aaaagcagtt gctggtttcc ttcttgaaaa cgaaattgcc tacatccaag
     5641 aagcagttga aactccagaa cgtccattcg tggctatcct tggtggttca aaagtttcag
     5701 acaagatcgg tgttatcgaa aacttgcttg aaaaagctga taaagtcctt atcggtggtg
     5761 ggatgactta cacattctac aaagcacaag gtatcgaaat cggtaactca cttgtagaag
     5821 aagacaaatt ggatgttgcg aaagctcttc ttgaaaaagc aaatggtaaa ttgatcttgc
     5881 cagttgactc aaaagaagct aacgcatttg ctggttacac tgaagtgcgt gacactgaag
     5941 gtgaagcagt ttctgaaggc ttccttggtc ttgacatcgg tccaaaatct atcgccaaat
     6001 ttgacgaagc tttgactggt gccaaaacag tcgtatggaa cggacctatg ggtgtatttg
     6061 aaaacccaga tttccaagct ggtacaatcg gtgtgatgga cgctatcgtg aaacaaccag
     6121 gagttaaatc aatcatcggt ggtggtgact cagctgccgc agcgattaac cttggccgtg
     6181 cagacaagtt ctcatggatt agtacgggtg gtggagcatc aatggaactt cttgaaggta
     6241 aggttcttcc aggacttgca gccttgacag aaaaataaga ttttataaat aaatcaaaga
     6301 agagagggat gaaagttcct cttttctttt gcttaaaata aaaacgcttc ctctcaacta
     6361 ttactcataa aaatcaccga tttatgataa aatgga
//
LOCUS       DAVMEF010000111         6251 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMEF010000111 DAVMEF010000000
VERSION     DAVMEF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.111"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            112..1491
                     /gene="glmU"
                     /locus_tag="VYQ30_001548"
     CDS             112..1491
                     /gene="glmU"
                     /locus_tag="VYQ30_001548"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1966628.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1501..2046
                     /locus_tag="VYQ30_001549"
     CDS             1501..2046
                     /locus_tag="VYQ30_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1966629.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            2058..2372
                     /locus_tag="VYQ30_001550"
     CDS             2058..2372
                     /locus_tag="VYQ30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1966630.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2389..3081
                     /locus_tag="VYQ30_001551"
     CDS             2389..3081
                     /locus_tag="VYQ30_001551"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1966631.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3158..3649
                     /gene="rocS"
                     /locus_tag="VYQ30_001552"
     CDS             3158..3649
                     /gene="rocS"
                     /locus_tag="VYQ30_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1966632.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3747..4337
                     /locus_tag="VYQ30_001553"
     CDS             3747..4337
                     /locus_tag="VYQ30_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1966633.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4346..6109
                     /locus_tag="VYQ30_001554"
     CDS             4346..6109
                     /locus_tag="VYQ30_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1966634.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         1927 a         1053 c         1373 g         1898 t
ORIGIN      
        1 atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag agtgcggaat
       61 gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat tatgtcaaat
      121 tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt gccaaaagtt
      181 ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt gggagctatc
      241 caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga ggaggtcttg
      301 gctgaacaga cagaatttgt gactcaatct gaacagttgg gaactggtca tgcagttatg
      361 atgacagagc ctatcttaga aggtttgtca ggacacacct tggtcattgc aggagatact
      421 cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa tcataaaaat
      481 gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg aattgttcgt
      541 aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga ttttgaaaag
      601 caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt gtttgaggct
      661 ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga cgtcattggt
      721 attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt tgatgaaagt
      781 cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg tcgtcgcatc
      841 aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac ttatatcgat
      901 attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttatctt gaaagggcaa
      961 acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga cagcactatc
     1021 ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc agacggtgtg
     1081 acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca agttcatatt
     1141 ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc tggtcatttg
     1201 acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg aactattaca
     1261 gtcaactatg acggcaaaaa caaatacaag acagtcattg gagacaatgt ctttgttggt
     1321 tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt tggtgctggt
     1381 tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg tcgtcagatc
     1441 aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta ggagcctatc
     1501 atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc aatatttaaa
     1561 ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccgacg ggatttgatt
     1621 ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact tatcttggtc
     1681 aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg aaaattggaa
     1741 gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga agaaacagcc
     1801 tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt ttgtaatgag
     1861 aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg tccgcaggat
     1921 gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga attaatccaa
     1981 tcaggtcata tctgtgatgc caagacaatt atggctgttc agtattggga gttgcagaaa
     2041 aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt gaaagagcta
     2101 atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggaa actaagattt
     2161 taccaacctc ttcttcccgt tttggttatg ccaatcctaa ggatcatggt tttagccagg
     2221 aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgtatt gaaaatacca
     2281 agagaaatgt cttcaattct aagttgaata aaatcttatt tgcggtcatc tttctcttga
     2341 ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat gaaaatagga
     2401 attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga taatacccag
     2461 gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca tgaagtcgtt
     2521 cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat tttggctgat
     2581 catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc agaaggtatc
     2641 gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga tgtcacagct
     2701 tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc agacaaaacc
     2761 tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca tcttggtttg
     2821 attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat taagtcccat
     2881 ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc agcgcatgcc
     2941 ctcaatctcc cagtcttagt catcagagct atgagtgaca atgccaacca tgaagcaaac
     3001 atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt cttgttgacc
     3061 tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat gataagattt
     3121 aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa tgaccgtcag
     3181 tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg tcaaagaatt
     3241 accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca gaagtggctt
     3301 gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgagcctg tcagtgaaga
     3361 tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag aaattcttcg
     3421 tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg agcagatgcg
     3481 tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac aacaattgac
     3541 ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag caaaagaaga
     3601 agtccaatcc actaagaaag gcttttttgc tcgtttattt ggaggataat caaggagctg
     3661 tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag ttgattagaa
     3721 cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt gcttttgatt
     3781 tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca gtgcgtttta
     3841 gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc cctctaaaaa
     3901 gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct cctccagttg
     3961 agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata ggctacaatg
     4021 ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc caacagtaca
     4081 aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat ggtattgcca
     4141 atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct cataatagtt
     4201 tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg gatcaagcta
     4261 gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtggc ttggacttgt
     4321 ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat tgggtcttaa
     4381 acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt ttatgatagt
     4441 ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag atcagacagg
     4501 ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc cattgattag
     4561 tctagctttt ttagccttga ttgtaattaa ccttctggtt gcttattttc aaataggact
     4621 cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa ttgagtatat
     4681 ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag ctaacttgcg ctaaagcctt
     4741 gtttatcttt ttctatatag ctatgctttt tccttttata cggaaaatgc tgaagattta
     4801 ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag ataaatattg
     4861 gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct ctgtcagatt
     4921 gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag cagtgatgtt
     4981 tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat tttacatctt
     5041 gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg ctcaaacttt
     5101 ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt ttgtcgtgat
     5161 taaaaatctc tattatatgg ctctaactta ttttttggtt aaatttgttt catttttgac
     5221 aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc gatggggtgt
     5281 catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg aggctcccat
     5341 ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg ggattcaaaa
     5401 tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg agacggacgt
     5461 gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga aaaatctagc
     5521 aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga ttgatatttt
     5581 tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc gagccaacga
     5641 acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc cagacatgat
     5701 gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc aaatccagtc
     5761 tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc cagtttattt
     5821 catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata ctatggagta
     5881 ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga aactttatgt
     5941 ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag tagatggagt
     6001 gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag aagatccaga
     6061 atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt aataaaaaag
     6121 aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc ttttttgtgg
     6181 ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt tcaatttact ttatagtgct
     6241 ttgaagttgg a
//
LOCUS       DAVMEF010000112         6250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMEF010000112 DAVMEF010000000
VERSION     DAVMEF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.112"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..441
                     /locus_tag="VYQ30_001555"
     CDS             <1..441
                     /locus_tag="VYQ30_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1966635.1"
                     /translation="SFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELI
                     KYKHLRINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLP
                     LPPLALQNEFADFVVQADKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            501..737
                     /locus_tag="VYQ30_001556"
     CDS             501..737
                     /locus_tag="VYQ30_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1966636.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            734..1147
                     /locus_tag="VYQ30_001557"
     CDS             734..1147
                     /locus_tag="VYQ30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1966637.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1265..2230
                     /locus_tag="VYQ30_001558"
     CDS             1265..2230
                     /locus_tag="VYQ30_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1966638.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLSYAMVNQNNVKHSHQKFIS"
     gene            2253..2834
                     /locus_tag="VYQ30_001559"
     CDS             2253..2834
                     /locus_tag="VYQ30_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1966639.1"
                     /translation="MFEEYPDSVFLDTYIKELRAGKSLAGEENNKNKVLKTGAVSYDY
                     FNSSEVKNLPIDYIPLDEHKVEIGDVIISRMNTSELVGAAGYVWAINSDNIYLPDRLW
                     KVILNDRVNPVFLWKLITNEKTKLKIKRISSGTSGSMKNISKSQLLQIRVPFPPLALQ
                     NEFADFVAQVDKSQFACEIAIKVWRNSLKFSII"
     gene            2864..6214
                     /locus_tag="VYQ30_001560"
     CDS             2864..6214
                     /locus_tag="VYQ30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1966640.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFMERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         2103 a          990 c         1260 g         1897 t
ORIGIN      
        1 tcattcgata caaagcaatt tatcactaaa acaaaggata aattacttcg aaaaggcaaa
       61 cttgagcgtt atgatatagt cttgacaaca agaggtactg ttggaaatgt agcgtactac
      121 gatgaattaa taaaatataa acatttacgt ataaattcag gtatggtaat attacgtccc
      181 aagacaccaa atctaaatca gaaatttatt atccatgttt taaggaataa taattatagt
      241 cgagtgatat caggaagtgc tcagcctcag ttaccaatta caaaattaaa aaaaatactt
      301 ctccccctcc ccccactagc cctccaaaat gagttcgcag actttgtagt ccaggccgac
      361 aaatcacaat tggcaatcca aaaatctctg gaagaacttg aaactttgaa gaaatctctg
      421 atgcaggagt attttggctg atattctgcc attgtaatta cggtaatgat ttgttataat
      481 acttcaaagg aggaaatcag atggtagtaa aaacaagaaa acaaggaaat tcaatcacca
      541 ttacgattcc aagtgaattt aatattccaa gtggtgttaa atacgaagcg aaattgttac
      601 caagtggtga gattatcttt actcctgaag aattggggca gcaggtttct tatgtatctg
      661 atgatgcctt tgacttaaat ttagataaaa tatttgacga atacgacgat gttttcaaag
      721 ctttggtgga aaaatgacaa tctatctgac agaaaagcaa attgaaaaaa taaatgcttt
      781 agcaattcaa cggtattctc caaatgagaa aattcaaaca gttagttctt ctgccttaaa
      841 tatgattgtg aacttaccag aacaatttgt ctttgggaag tctctttatc caacaatttt
      901 tgataaagca acgatactat ttgtccaatt gataaagaag catgtttttg ctaatgctaa
      961 taaaagaact gctttcttcg ttttggtcaa atttttacaa ttaaacggct atcgtttttc
     1021 tgtaacggta gaagaagcag taaaaatgtg tgtaaccatc gcagtagaag ctttaactga
     1081 tgaaaaaatg acaagctact ccaaatgggt ttctgaacat tctgttagag aaaaggtcaa
     1141 aaagtaacct agtatgctgg atttgaatga gcacaagaaa ataaatgaac agacaatatt
     1201 agaattctgt aatgcagaaa ctgatattgt ctctttttat tgatgaataa gaaagtgaga
     1261 aattatggaa tcaaaagtta caattatcat gcaagaaatg ttacctcttt taaataatga
     1321 acaattacta gcgttgagag agagtttaga acatcatcta gtagacggaa aaaagcagca
     1381 gaagtattcg aataataacc tgttgcaact atttattacc gccaagcagg tagagggctg
     1441 tagctcaaaa acaattcgtt attaccagag gacgattgaa aacttgttta atgctattaa
     1501 agagtctgtg acacaactca caacagatga tttaaggagt tatttagcaa attaccagtc
     1561 tgaaaaggat tgtagtaagg caaatttaga caatattagg cgtatattgt cttctttttt
     1621 tgcttggctt gagcaagagg aatatatcat taaaaatccc attcgacgga taaagaaaat
     1681 taagactgag caaaatgtga aggaaactta tactgatgaa catttggaaa ttatgcgtga
     1741 taactgtgaa aatttgagag atttggcaat aatagaccta ctagcatcga caggtatgcg
     1801 tgtaggggag cttgtacagt tgaatcgttc agatattgat tttgaaaaca gagagtgtgt
     1861 tgtctttggt aaaggaaaga aggagagacc agtatatttt gatgctcgga cgaaaattca
     1921 tttaagaaat tatcttaacg acagaaaaga tagtcaccct gctctttttg taacgctagt
     1981 tggaaaagtc cagagacttg gaattgctgg tgtagagatt cgcttaagaa agttaggaga
     2041 caaactcggc atacaaaagg ttcacccaca taagttcaga agaactttag cgactaaggc
     2101 aattgataaa ggtatgccta tcgaacaagt ccaaaaactg ctaggtcata gcaagattga
     2161 cacaaccctg tcctatgcca tggtcaatca aaataatgtc aagcattcac accaaaaatt
     2221 catctcttaa aagcaaatcc cgatttaacg agatgttcga agagtatcct gactcagtat
     2281 tcttagatac atatataaaa gaactaagag caggtaaaag tttagctggt gaggaaaata
     2341 ataagaataa agtattaaaa acaggagctg tatcgtacga ttattttaat tcatctgaag
     2401 taaaaaattt accaattgac tatattccct tagatgaaca taaagttgaa ataggggatg
     2461 taattattag tcgtatgaac acttctgaac tagttggggc tgctgggtat gtttgggcta
     2521 tcaatagtga taatatatat ttgccagata gattatggaa ggttattctg aatgatcgag
     2581 taaatccagt tttcttatgg aagcttatta caaatgagaa aacaaaatta aaaattaaga
     2641 gaatttcctc tggaactagt gggagtatga aaaatatttc gaaatcacaa ttgttacaaa
     2701 ttagagttcc cttcccccct ctagccctcc aaaatgagtt cgcagacttt gtagcccagg
     2761 tcgacaaatc acaatttgct tgtgagatag ctataaaagt gtggagaaat agcttgaaat
     2821 ttagtataat atagctaaac tatttgttta aagtgagaaa aaaatgggaa attttagctt
     2881 tcttttaaaa aatgacgaat atgaatcttt ttcaaaacct tgcattgaag ctgaaaatat
     2941 gattgctaca tcaactgtgg ctactgcctt tatggcgcgt cgtgctttag agcaggctgt
     3001 ccattggata tatagtcacg attcatattt agaagctccc tatcgtgcta ctctatcttc
     3061 tttagtatgg gatgatgatt ttagggatat cgtagattct gaactccaca agcagatagt
     3121 tctgttgatt cggtggggaa accatgctgc tcatggtggt gaaattaagg aacgagaagc
     3181 gattttagct ttgcatcatt tgtatcagtt tgttaatttt atcgattatt gttacagcaa
     3241 tgagtttatg gagcgttatt ttgatgagaa gtgcttacca ctttcagcaa acatcaaata
     3301 ccgagaaact ccacaatcta tgataaagtt acaagacagt ttaccagaac tgcctgattt
     3361 tcatgaacag atggctgctc agtccgtaga agttcaagag acttatactg aaaaacgtga
     3421 gactgcagcg caacggcaag atgtgccttt ccatattgat caattatctg aggcagagac
     3481 aagaaagctc tttattgata tcgatctccg tttagcagga tggatatttg aagaaaactg
     3541 tcgtgttgag atagccgttg atggtctcaa gcacggttca ggaattggtt actgtgacta
     3601 tgtactttat ggtaaaaatg ggaaaatttt agcgattgtg gaggctaaaa aagcctctgt
     3661 caatccagaa gtaggggaag tacaggtcaa agaatatgct gaagctttgg agaaacatat
     3721 cggctatcag ccaatttgct ttattacaaa tgggttgaag cactatatac ttgatggtcc
     3781 gaaccgccgc cagattgcag gcttttactc tcaagaagaa ttgcaattag tgatggatag
     3841 acgtcatctt caaaaaccgc ttgaggatat ttctagtaaa attagggacg atatttccgg
     3901 gcgtcactac caaaaacatg ccattgcaag cgtttgtgaa gctttctctg atcatcgtag
     3961 acaggcactt ttggttatgg caactggggc ggggaaaact cgtacagcag tttctctagt
     4021 tgatatctta tcacgtcata actgggtaaa aaacgttctc ttcttagccg atagaacttc
     4081 cttggttaag caagcatatg attcgtttag aaaattactc ccagatcttt ccgtttgtaa
     4141 cttcttagaa gataaagaag gagctcaatc aagtcgaatg gtcttttcaa cttatccgac
     4201 catgattgga gcgattagtg gtcaagaaga agtaaatcaa cgccctttta ctgttgggca
     4261 ttttgacctt atcataattg acgaatctca ccgttctatt tatcagaaat acaagtccat
     4321 ttttgattat tttgatgcaa ggattgtagg cttaacagct actccgcgtc aagatttaga
     4381 taaaaacacc tatggattct ttaatttgga gaatggggtt ccaacatatg catatgattt
     4441 ggaagaggct gttaaagacg gatatttagt agcctatcat tctatcgaaa ccaaactgaa
     4501 actacctacg gatggtctac attatgatga tttgtccgaa gaagaaaagg aacattttga
     4561 tagcaaattt gaagacgata gctgtgaaaa agatattgat gggagtgtgt ttaattcctt
     4621 tattttcaat aaaagtacag tagaaattgt tttaaatgaa ctcatgacaa gaggaattca
     4681 gacagcctcg ggtgatgaaa ttggtaaaac tattattttt gctaaaaatc atgaccatgc
     4741 ggaatatatc agaggtattt ttaacaaccg ctatcctgaa aaagggagcg actatgctca
     4801 ggtgattgat tatagtatta agcattatca gaccttgatt gatgatttta aaattaagga
     4861 gaagtatcct caaattgcga tttctgtcga tatgttagat acaggtattg atgtaccaga
     4921 ggttgttaat ttagtcttct tcaagaaagt acgctctaaa actaagtttt ggcagatgat
     4981 tggtcgagga acccgtctat gtaaagattt atttggacct gagcaggata aggaaaactt
     5041 cttggtattt gattatgggg acaattttga ttattttcgt gcagatccaa gagatggaga
     5101 gggtcgtcac attgtttcgc tgactcagcg tttatttaat atcaaagtgg acttgattcg
     5161 agaacttcag ggactccaat accaagaaga tcagtttgcg agagcatacc gtcagcagct
     5221 tgtctcggaa cttcaaggtc gtatagagag cttaaatgag ttggacttca gggttcgtat
     5281 ggttttagat acagtttata gctataggaa attggaaagt tggcagaatc taactgctgt
     5341 tacaagtgaa accattcaaa aaaatctctc tccgctttta tttgatgaag ataaagaaga
     5401 tgagatggcg aggagatttg atttgtggtt gcttcatatt cagttggggc aactgacagc
     5461 taaatcttcc actgttcata tttcccaagt gatgaagacg gctagagctc tttctgctat
     5521 tggcaatatc ccgcaggttt ttgagcaggc tgaaattatc aggaaagtac aggagcctga
     5581 attttggaaa gaagttaact tgtctgattt ggaaaaaatt cgtcttgcta ttcgagattt
     5641 attacagttt ttggataaaa cagaccgtaa accctactat gttaactttg aagatcgtat
     5701 actctccact gttcacgaga ccacagcatt tttgcaggtc aacgatcttc ggtcttacaa
     5761 tgaaaaagtt gagcattatt tgaaaactca tctggatgag gagtccattt ctaagctata
     5821 ccataataaa aagttgacat ctgatgatat gcttgcactt gaaaaattgc tttgggaaaa
     5881 attaggtagt aaagcagact accaaagtca ttatgaaaat aaggcaattc cgagattggt
     5941 tcgtgagatt attggcttag atagagagtc tgccaatcgt attttctcta aatttttgtc
     6001 ggatgagaat cttaatgcca ggcagatttc atttgtaaaa ttgattgtag actacattgt
     6061 agaaaatggt tttttagaga cgaaagtgtt aacgcaagag ccgtttaaat cttatggttc
     6121 tgttcaacta ctcttccaac accaactacc agtacttcgt aatattgttc aaatcattga
     6181 acttatcaat aatcgagctg gagaagcggc ttaaattcta aagtgattgc catgctgaga
     6241 ctcatttaaa
//
LOCUS       DAVMEF010000113         6137 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMEF010000113 DAVMEF010000000
VERSION     DAVMEF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.113"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(54..329)
                     /locus_tag="VYQ30_001561"
     CDS             complement(54..329)
                     /locus_tag="VYQ30_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1966641.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFVAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(436..1275)
                     /locus_tag="VYQ30_001562"
     CDS             complement(436..1275)
                     /locus_tag="VYQ30_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1966642.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1417..2265
                     /locus_tag="VYQ30_001563"
     CDS             1417..2265
                     /locus_tag="VYQ30_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1966643.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2358..3275)
                     /locus_tag="VYQ30_001564"
     CDS             complement(2358..3275)
                     /locus_tag="VYQ30_001564"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1966644.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3712..3917
                     /locus_tag="VYQ30_001565"
                     /pseudo
     CDS             3712..3917
                     /locus_tag="VYQ30_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4228..4521)
                     /gene="rpmA"
                     /locus_tag="VYQ30_001566"
     CDS             complement(4228..4521)
                     /gene="rpmA"
                     /locus_tag="VYQ30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1966645.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4538..4882)
                     /locus_tag="VYQ30_001567"
     CDS             complement(4538..4882)
                     /locus_tag="VYQ30_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1966646.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4898..5212)
                     /gene="rplU"
                     /locus_tag="VYQ30_001568"
     CDS             complement(4898..5212)
                     /gene="rplU"
                     /locus_tag="VYQ30_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1966647.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5248..5335)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5394..6098)
                     /locus_tag="VYQ30_001569"
     CDS             complement(5394..6098)
                     /locus_tag="VYQ30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1966648.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
BASE COUNT         1879 a         1262 c         1031 g         1965 t
ORIGIN      
        1 aagcctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
       61 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      121 atttctttac cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag
      181 ttaccaaaac cgatcaactg aactttttca ccagctgcaa gatagtcagc tactgctaca
      241 aatacagctt caactgctgc tgctgagtct ttcttagtta attctgtagc ttctgctact
      301 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta
      361 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc
      421 attttattta tttttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      481 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      541 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      601 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      661 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      721 gatattgaga agagagctca acaagccagt tacacggcta attcgtccac ctttgacaag
      781 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      841 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      901 gaatttcagg gcttggtcag tgaaggaact atcaacaaca gtcacatctg cagtagatag
      961 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1021 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1081 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1141 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1201 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1261 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1321 ctaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1381 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1441 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1501 catcatctct ttgaaggagg ggcgacaggc attaccctca tcacctttta tctttttaaa
     1561 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1621 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccgcctgg
     1681 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaatcaca
     1741 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1801 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1861 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1921 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     1981 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2041 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa
     2101 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2161 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2221 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2281 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2341 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2401 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2461 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2521 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2581 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2641 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaaccacc
     2701 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2761 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2821 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2881 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     2941 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3001 tcttctacac cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa
     3061 aacaaatctg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3121 acgataggta ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3181 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3241 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3301 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3361 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3421 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg
     3481 tcatactatg ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3541 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3601 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3661 tcactttccc taatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3721 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3781 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3841 aagccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3901 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca
     3961 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4021 aggctgtact tgagtacggc aaggtgaaat tgacgtggtt tgaatttgat tttcgaagag
     4081 tatcaatcca ctatacatat taaaaagtat gggtatcaaa ataaaaaaac aggctctccg
     4141 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4201 tcgggataaa aggttcaatg aaccttttta ttttgcgatt gggtaaacag aaacttgttt
     4261 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4321 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4381 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4441 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4501 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4561 tttggacgaa ctcagaagag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4621 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4681 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4741 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4801 attcgccact ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc
     4861 tctcaaagac tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga
     4921 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     4981 acttgtaagt aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5041 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5101 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa
     5161 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5221 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5281 gaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5341 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5401 gaagcttttt cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg
     5461 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5521 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc
     5581 aaaaaaggag tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata
     5641 ttccctgtca taaaagcgtt attataggca atacccgaca cttctccaaa agcagttgtc
     5701 accagtccca tacagaaggc caagggcggc actagatata tattatccac agtttgcggc
     5761 acaaaagcaa taatgattga taagattgcc aagggaatca aggacagaat aggttttttc
     5821 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc
     5881 aaggtgagaa ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga
     5941 aagacaacat ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa
     6001 gcatccacat atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat
     6061 atttttctta taggtaataa cctcatttta cctcccattg tattttctct tagaaatatt
     6121 gtaccatttt ctttcta
//
LOCUS       DAVMEF010000114         6103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMEF010000114 DAVMEF010000000
VERSION     DAVMEF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.114"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(80..1168)
                     /locus_tag="VYQ30_001570"
     CDS             complement(80..1168)
                     /locus_tag="VYQ30_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1966649.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1186..1854)
                     /locus_tag="VYQ30_001571"
     CDS             complement(1186..1854)
                     /locus_tag="VYQ30_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1966650.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYYITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGVDVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1873..4311)
                     /locus_tag="VYQ30_001572"
     CDS             complement(1873..4311)
                     /locus_tag="VYQ30_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1966651.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4462..5760)
                     /locus_tag="VYQ30_001573"
     CDS             complement(4462..5760)
                     /locus_tag="VYQ30_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1966652.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(5777..6085)
                     /locus_tag="VYQ30_001574"
     CDS             complement(5777..6085)
                     /locus_tag="VYQ30_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1966653.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
BASE COUNT         1991 a         1289 c          907 g         1916 t
ORIGIN      
        1 atcttatttc aatctgctat agaattcaat acgaggatca ataaaagact accatttgga
       61 aaacagtagt ccttattgtt tattttgaat ttacataggc atctacagcg ataatagctt
      121 gagcaacatc tgaaggcgaa atcttaaacg gcatctgatg aattgtctca ccctccatag
      181 ttgcttgttt accaactttt attaaatcat catatccaac ttgatccaaa tgcatttctt
      241 ttagagttgt tggcatacca atttttttgt aaaactcaat atacttatca agttcttctt
      301 taggtctatt ttccaataat agttgtacta aagttccata agctactttt tcaccatgtg
      361 ttaaatgatg aatgtcacct gtcaatgcag taaaaccatt atgaattgca tgcgccgcag
      421 ctaatcctcc actttcaaaa cctaggccac tcaataaagt attagcttca acaatatttt
      481 ctaatgctgg tgtcaccact ttagcttcac aagctgccat agcctgtaaa ccatctgcaa
      541 acagcgtttc ttcacatttc ttcgcaattg caactccagc caatgtttgc tgttgtccca
      601 acatagtttt tccatttgcc tgcataaccg cacgcgcctc aacccaagtt gctaaaccat
      661 ctgcaatacc agacgctaat aaacgcttag gggcttgtga aataactttt gtatcaacca
      721 aaactaaatc tggattttta gaataaaata gataatgatc aaatgcacct tcatctgtat
      781 aaataacaga taaagcagat acaggtgcgt cggtcgatgc aattgttgga gcaataataa
      841 caggcttttc aatcaaatct gcaatagctt ttgcgctatc aatcgtcttt cccccaccaa
      901 gaccgataat actatcacaa ttttctttct cagccaaggc aacaactcga ttgatttcat
      961 tgtcagaagc ttcaccatta aatagcgcca gaacaatatg gaaaccatac ctatgtagat
     1021 aatcttcaaa tcgttttcca acaatatcat aaaccaactg atcgcataat agaatagggc
     1081 aatttcccaa atccaaaatt gatttggcat tttcaaacaa ggcattttcc ccctgaatat
     1141 atctagaagg actagcaaaa attctcatat gtatttcctc tctatctaaa tggaacgact
     1201 attttgaata acaaaccaat cgtcagaaaa atcatcaacc gccttttgga tagatggcat
     1261 ggcgaaagct gattcaaaaa catctactcc tgctgtaaca gcatgcgcac ctgcagctaa
     1321 agcattattt acttgtgcta catttttaaa ggatgcagct aaaatcttac taggagagtt
     1381 ctgtctatca atagcaagag ctaattgacg aatgacagaa tttgaatcaa tgttcagatt
     1441 ttccattcta ttataatatg gagctaggta atccgctcct gcttcgatag ctaataatcc
     1501 ctgaataact gtataaatag ctgttgcagt gatatagtag ccctcttttt ttagcacctt
     1561 tattgcacgt aatccagccg gagtaacagg tactttgata aatatatcat ctcctgcttg
     1621 tcttcgaatt tcatgagcat cctttaagat gccttcaaaa tcttgagaaa tcacctgaac
     1681 atgaatagag ggtgtagagc caatcaattc tcttacatct ttgattcgtt caaaaaaatt
     1741 aatagaaccc tctctttttg caatagtggg atttgaagtt accccagcta gcggcaaaat
     1801 ttcagaccac tttttaatct catctaaatt taatgtgtca agcataaatt ccataaaaag
     1861 aacctcttta ttttacaaag tatgctcagt acgtcctata atgtcatctt gggttgcctt
     1921 agaaagaaca ttgaagaatg cagagtatcc tgcaacacga acaattaagt ctctgtgttt
     1981 ttcaggatgt ttctgagcgt caatcagcgt ctctctggaa acaacattgt attgaatatg
     2041 gtacccatgt aaacgattaa agaatgttcg taacaaagca attagtttta atttatcttc
     2101 ttctttggct aacgtttgag gatttacttt ctgatttaag agaaccccac ctacgatttc
     2161 atctgttggt aattttgaaa cagattttaa aacagatgta gggccgtgtt gatccatatt
     2221 atgtgatggt gaacaaccct ctgctaacgg tgtacccgcg ttgcgtccat ctggagttgc
     2281 taatgttcca cgtccctgcc ctacgttggc tgagatagaa gatgttcctg aataacgaat
     2341 tcctccaatt ggacctcttc cataacgtgt attaggatat ttagcaattt catcaacata
     2401 aatgtcataa gcagcagtaa ccaatttgtc agcataatca tcatcattac catacttagg
     2461 tgcatcatga atcaacattt cttgaatgac cttaccttct tctccggcat aatctgtttc
     2521 cagtgcatgc caaagttgac ttgggcttat acgttcttcc tcaaacacca attttttaat
     2581 tgcagctaac gaatccgaca aatttgcaat tccaacttgc aatcctgata tataatcata
     2641 tactgctcca ccttctttaa ggtgttttcc acgaccaata caatcatcag tcaatgctga
     2701 acatagaata tcaggaactt ctcgttccaa tgataaatca atagaatttt caacaataac
     2761 actcattcgt gtcaaatatc ttagtgtttt atcccaagca ttttctaatt cagaaaagtt
     2821 cttcatatcc ttaaaatgac caaagcttgg tgcaaaccgt ttacccgaag ccggatcaat
     2881 tccatcattc atcgtgataa gtagaacctt agggaagttc atataactca tacctgtgca
     2941 acgatagccc catttccctg gaactgccgt ttcaacacat ccaatggcac tgtaatcata
     3001 agcatcatct tccaatactc cttttgcaat aaaagaagga ataataatct catcattatt
     3061 aaatgcaggc ataccaaaac caagtttcat cacttcaata cactcattca tgaaacgagc
     3121 atctaaacct gcatggtaac gtacagttag attaggttgc ggtagatggg tttgtgcaac
     3181 tgattttaat accaaataag ataatgggtt aacagcatcc ttcttatctc gagtctgtcc
     3241 accaattgta acattttgat ataaaggact tcctgctgaa gaaaatgtat gtgattgact
     3301 gcgaacctta ttaattgtaa ttgtcttaat ccaaagattt gtcagacgtt caacaatgct
     3361 atcttctgtt tctttaccac tttctaaatc agccttcata tatggataca tatattgatc
     3421 aaaacggcca tatgaaagag agtggccatt agattcaatt tgtaaaatac attgaataaa
     3481 ccaaactgat tgaatagctt ctgcaaaagt agttgccggt tcatatggga ctctagagca
     3541 aatatctgca atctcaagta attctttctt acgtttagga tttgcatttt cggctaaact
     3601 tttagcaaga gcaacaaagc gctttgcata tactttaata gcatcgatta cgataaatat
     3661 agagtcgtaa aaatgatatt tatcaatact tgctggatct gttaaatcta gagctacttt
     3721 tgctttacga gcccgctctt caaaacctct taaaccaaat tgcaaaagtt tctgatagtt
     3781 aactgctaag tgagcatctc cagaattcat cttaccttcc ataccgaaga atcctgtttc
     3841 catataaact gacacttctt caggtaataa ggcaccagct ctagcacgta aattattatt
     3901 ttcccaaaac ggagcaatac ttctaagttg ttcttttgtt tcttctgtaa tatagaaaac
     3961 atctccatca cgcttttcaa aaagatccaa ctcattgaga acaaattcta gcgtatattc
     4021 cggaaaaata ggagcatctt tattggaaga agcttgattt cccgcaatca tagattcttc
     4081 ttcaatatag atagacatat tttccaaaat ttctttcagc atatatgcac gttttaggac
     4141 attaggttgt tccttgtatc gttcataggc gcgtgttgct agaactgctc tctcagcatc
     4201 aatataaggt tttttattta aaacatcttc tcgatatttg ttcatccttt cagtaaggct
     4261 gccaaaatat tctgtcttga ttgttgttct tgctacttct gtatttacca tcgtaagcac
     4321 ctttctttcg aaaatatttt tatttctttc gaaaatataa taccgatttt tatagaaatt
     4381 ttcaagcttt taactagaaa aattttaact ctctaatttt tagcacaaat gtgtgagttt
     4441 aacagtaaca cacaaatacc tctaagattg tttgatttca ttttggtaag ctaaacgatc
     4501 ttgtatctta aagaatggaa agtaaaccac tgtagatatc accaatacta ataactgagt
     4561 cacaacacct tgccatccac ccaccaaaaa tcctgataga atagctggtg tactccaagg
     4621 caatgttacc cctgagaatg gctgcatgaa acctgttgca atagctccat atactatcac
     4681 agctgcaagt acaggaacaa gaatgaaagg tacaaacata actggattca tgacaatcgg
     4741 aaatccaaat acaactggct catttacgtt aaatattgct ggaaaagctg caacttttcc
     4801 taaggcttgg tattgttttg attttgctgc aaaaagcatg gcaactacaa gaccaaacgt
     4861 aatccctgaa cctgatagaa ttaaaaatga atctaaaaat tgttgagtaa caatatgtgc
     4921 accattttct aatgatagat tagcagaggc taacatagct ttattagcgt caagattaga
     4981 taaaagcaga gctgtcacta ctccatttac taccgattgc ccatgaacac caaaccacca
     5041 caaaaatgat ataaagaatg caattccaat agcaccatac aaagatccag ttaaaccttg
     5101 caacggaact tgaatagcag aataaatcat ttctatgaat gttccgccat tagtcaatga
     5161 cttcgctaaa atatatacaa tcatagaaga taagaaaatt acaaatgctg gaatcattgc
     5221 ttcaaactgt ttggcaatag cttgtggaac ttgttctggc atcttaataa caatttttct
     5281 ctttataaag aaggtataaa tacttcctac taccaaacct ataatgatag caccgataat
     5341 tccttggcct ccaaaccaaa ctttactaat agcgtcccca atcgcctcac cttgtttagg
     5401 gatataagat gatcttagca aaataaagaa tgcagataca gatagaactc cagctggtaa
     5461 agcctctact ccgctattct tagcataaga ataggcaatt gaaaaacaag aaattagacc
     5521 cataatagca aaagttcctg aatatacttg cataaacggc tctgtccaat tagctccaaa
     5581 aacactagca atgctcttat ttaatccttc gaacggcaat tgtcccataa tcaagaacaa
     5641 actaccaact actgtcaatg gcaaaattgc taacatccca tcttttagag ctataatgcc
     5701 acgcatattc acaaacttca tcatcggtgc aatgattttc tgaacatcca tctttgacat
     5761 aataaatctc cttttcttac ccactaatca aagatagggc caaatctaat acttttttcc
     5821 catctaacat accatagtcc atcatcggaa taacagctat cggaacatca cacttatcac
     5881 aaatttcttt tgatttatct aatgtataag caacttgtgg acccaatagt gcaacatcta
     5941 tatttggcgc ataatccgct aatttagact gagaaaacgc ctctatttct gcctcaactc
     6001 cactagcttg cgctgcaatt ttcatattat ttacaagcat accagtagaa aaacctgctg
     6061 cacaaaacaa accaatcttc accattatgt tttcctcctc tat
//
LOCUS       DAVMEF010000115         6085 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMEF010000115 DAVMEF010000000
VERSION     DAVMEF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.115"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            13..141
                     /gene="blpC"
                     /locus_tag="VYQ30_001575"
     CDS             13..141
                     /gene="blpC"
                     /locus_tag="VYQ30_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1966654.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(212..1552)
                     /locus_tag="VYQ30_001576"
     CDS             complement(212..1552)
                     /locus_tag="VYQ30_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1966655.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(1566..2303)
                     /locus_tag="VYQ30_001577"
     CDS             complement(1566..2303)
                     /locus_tag="VYQ30_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966656.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2308..2646)
                     /locus_tag="VYQ30_001578"
     CDS             complement(2308..2646)
                     /locus_tag="VYQ30_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966657.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2922..3251
                     /locus_tag="VYQ30_001579"
     CDS             2922..3251
                     /locus_tag="VYQ30_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1966658.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3419..4468)
                     /locus_tag="VYQ30_001580"
     CDS             complement(3419..4468)
                     /locus_tag="VYQ30_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966659.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4465..5196)
                     /locus_tag="VYQ30_001581"
     CDS             complement(4465..5196)
                     /locus_tag="VYQ30_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966660.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGKLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5264..5674
                     /locus_tag="VYQ30_001582"
     CDS             5264..5674
                     /locus_tag="VYQ30_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1966661.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5684..5971
                     /locus_tag="VYQ30_001583"
     CDS             5684..5971
                     /locus_tag="VYQ30_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966662.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         2005 a         1337 c         1118 g         1625 t
ORIGIN      
        1 gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact aacaactacc
       61 gaactcaacc aaattacagg tggaggattg tgggaagatt tattatataa cattaataga
      121 tatgctcatt acatcacata agaacttcat catccaatac aactataaaa aaataagacc
      181 gagaaacaag tactctcggt cttatttttc atcattctgt atgtatcaca gtaagtacct
      241 gacgaaagac ttgattttga caggtggtat ttagactggt attaggatgg ctttccacaa
      301 tcttcatgac ggtatagaga ccaactcctc tctcctcccc tttagaactg gctccaaagg
      361 agaagatttc agaaatatcg atgccctctt ctttgatgga gttttcgatg ataaaggtct
      421 cctgtgctcc attttttaaa aaggcgattg aaacatgagg ttgactagct tccacactgg
      481 cttcaatagc attgtcacaa aggatagaca caatggttag aaaatcaagt agactcatcc
      541 cctcgacctg aatctcctca ggaacttcga cattaaagac aatgttctta tctctggctt
      601 ttaaaaattt ccctgctaga agacttttga gggctttatc acgaatattt accaatctgc
      661 ccaggtcata tttattgttc tgcaatttct gactggaatc ctttaagacg gagccataga
      721 cctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta gtcaagaggt
      781 tggtataatc atgacgaaag ctccgtactt ccttgtaaag ctcctctata tgccgactat
      841 agcgttccat atctctatag cgcagggcct gctcttgttc caatctctca tagagttttt
      901 ccttcaaata ggtatccaat ttcttgataa cccccataaa aaagagtagg taaaagacta
      961 ggatgagatg gcgaacagtc tttgattgaa tactttgttc atattcaaaa aaagacagac
     1021 tttccatgac tagatagtag ccacccatta tccagttaat ctgagtcagg gacttttgaa
     1081 aggctttatc gagaatctcc tttctcaagc tagtaaaatc gtagtccaac catttcaaaa
     1141 aagctagaga aatgaagaaa ttgaaaatta ttatacataa cccagtaaat gagtagccat
     1201 catatacttg cccttgtccc aaaaatggaa gcacaaaata ggagactcct ctataaaaga
     1261 gattcaccaa tatcattgga aagagaccat aaaagaaaag gagtttttta ggaagccctc
     1321 tcaataataa gaaagataag cctatgccgt acaagggttc cataaaataa gataggtaaa
     1381 catttcctac tatatagcta atcatcacaa aaacaaaggc caacagtatc ttcaaaagaa
     1441 aggccttaaa aatcctctcg aaagtaagat caattccatc caccttaaag aagatgacaa
     1501 tttctagtcc attagtaaca agtgtataca acaatatcca agcaatgttc ataaattctc
     1561 ctagctcagt gtaatttatt gatggcctca gacacttccc tgaccttata acgggcgatt
     1621 agacaacttc caccattggg aaagaaaagc agtttttctt tcttatccaa atgcaccaca
     1681 tttgcaggat tgatgagaaa agagcggtgg cactgcaaga ggcggggctc ctgcttgaaa
     1741 acctcctcta aactcgctgt aaattccagc ctgtctgtct tggtatagag aataacacga
     1801 tgggctctgg gcgacgtttc gagatagtaa acctctttaa aaggatactg aaattgggca
     1861 aattttgatt taaagtaaaa gcaatcttcc gccagacttt tactatcttg actattggca
     1921 tagaggaggg ctgtctcgat ccgagattca aactcctctg ctgacaaggc cttatcaatg
     1981 tagtccaaag cagacacttg gtagcgaaaa gacaggggca taaactccga gtgagtcgtc
     2041 acaaagacaa tcagggcata aggatcccga tcccgaatct ttctagccac ttccagtccc
     2101 ttcatctctt catttcgaat ctcaatatcc aaaaagaata gctgatgggc ccccttctca
     2161 tgcacttcag ccagcagttg gtccggcttg ccaaatacct caaaagagct aggaatgata
     2221 tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg ggaaaaatca
     2281 tcctctaaaa caaatattct catcttctta ctctccctct ttcaaccatt tttgacgaat
     2341 ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc cgagaaaaag
     2401 gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac aatttcgatg
     2461 acatctcatc aaggtttccc cacattggtt ctcaagatta cttaaatttt gaagcatatt
     2521 aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat gtgtttggat
     2581 atagtagata tcgtcgatgt ttactttata gactgttttc tgcgtctgaa taatcatata
     2641 tttcattgga ttctcctcct aagaaaagaa taacagaaaa gatctagccc aagcacaccc
     2701 aatcctaaac agtctttgaa aactcctaaa aggattattt tctacaacat catgatgagc
     2761 ttacaactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
     2821 aagatttgaa taaaaaaaaa gaattaaaag tgataaacta acagtatcaa aacacagttg
     2881 ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat acagacccca
     2941 tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg
     3001 caaccgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca
     3061 agactgaaac agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca
     3121 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
     3181 cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat gaaaatcaaa
     3241 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaagacga cgctgacgtg
     3301 gtttgaagat attttcgaag agtataagtc tctacggacc aaccattatc ctgtagtaac
     3361 aaaaaagaag ctgagacaga ctgaactcaa cttctttttt agtgtcgtat cagcaatgtt
     3421 agtcctgcat ctgacgtttt acctgataag gcaaatacaa gactagtaaa accaaaccag
     3481 ctcctagtaa ggctagaagg gccagttttt cttggaaggt aatcaaccca acaactaatt
     3541 ccacaagtaa aacaaaactg gtcaatcctc gaactacctc ctgtaagcct ttttcctttt
     3601 gccccttgtc cagcggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg
     3661 ccagcaactg gaagagcaag aggtagttaa agagaactac cactgctgtc gcaatccaag
     3721 cttgctcgat aaaaacctgc gccagtacgg aaagtaagag aagacgaaga ctgagagcaa
     3781 agaggtcgcc atttcgcaga taagaacgca gatagagatt ttgccaaatc ttcccaggca
     3841 ccttctgaac agcctttaag ataaagtcca gataggcacg acgcttaacg ctgtttgaaa
     3901 ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa gacttgctta cgcttgcttt
     3961 cttgggaaat gacatagtcc cagtccagcc cagtttcagt gaaaaatttg ctggcctttt
     4021 gacgaaagtg gaaatatttt cctaccccca ataaaagcac atagagcaga aaaactggca
     4081 agccataacc cattgctaaa aataaaggcg caaataacag caagaaaagg gtctgtacaa
     4141 agagccaaaa gactagggaa atgccagttt gacgcttgag atggagcttg atttcctctt
     4201 ctccaactaa gagaaagagc ttgtctggag cctccatata ggtggcaatt cctccccaaa
     4261 gtaaaagtaa aacagacgta attcctacaa acaaaaggat aggccaatga ttttcaggaa
     4321 aatgttgtaa gagttgactg tactggtagg ctagaaagcc caacagtaca agcaggaaca
     4381 agacaaagtg gtcattgaga acatagcgca gataaccaag acactcctta cgaaaggcct
     4441 gctttctctt taaaaacaag tctttcatag gtcctcctct tttgtcagag ccaagtaaat
     4501 atcattcaaa ctagcctcag gcatatcaaa ggcttcgcgt agttgcagga gattgccttt
     4561 ggcacgcacc tctcccttgt gaagaatgac aaaggcatca cacatcttct ccgccgaatc
     4621 cagcacgtgg gtactcatga gaatagactt gcccttttgc ttctccactt ccaaaagctg
     4681 aatcaaatca gaaatagcca gcggatcaag accaaggaaa ggctcatcca cgataaagag
     4741 actcggatcc accacaaaag cacagataat catgaccttt tgcttcatcc cttttgaaaa
     4801 atgaacagga aaccagtcta atttctggtc cagacggaac atctttaaca agggctctac
     4861 tcgttcgaaa gccacttttt gctcaatacc ataagccata gcaaccgttt cgatatgctc
     4921 tctgagggtc aattcctcat acagactagg cgtctcagga atgtagccaa tctgcttgcg
     4981 gtagctagtc gcatctcctt gcagagtcag gccattgata ttgatggagc cactataagg
     5041 tgccaacaga ccgataatct cattgatcgt cgttgatttc ccagcaccat tgagaccaat
     5101 caaaccgacc aacttcccac tttcaacagt aaaggacaca tctttcaaaa caggaacatg
     5161 aacatagcca cctgtcaggt ttttaatttc taacatattt tctccaaatc tggtataatg
     5221 tagctatatt atatcaaaat tcagtacagt agaggtagat tttatgtcag attgcatttt
     5281 ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta tatgaagatg agcaggtcct
     5341 tgcctttctt gatatctctc aagtaacact aggacacacc ttggtcgtgc caaaagaaca
     5401 ctatcgcaat cttttggaga tggatgctac gagcgccagc caactctttg cccaagtacc
     5461 aaaagtagct caaaaagtca tgaaagtcac taaggctgct ggtatgaata tcatttccaa
     5521 ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac gttcaccttg tgcctcgcta
     5581 cagtgctgac gatgacctca agattgattt tatcgcccac gaaccagact ttgacaaact
     5641 tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct
     5701 actgctattt gcaggagctg cagccggaag ttatctggtt acaaaaaatc gccaaaccat
     5761 cacagatgaa gtcttgaata ccactgaccg cgttcaagct atcaaggacg atgtggatat
     5821 tatccaaaac agcctgcaaa tcattaacca gcaaaaagaa cttatcaagg aataccaaga
     5881 agacttgact tacaagttta aggtcttgga aaaggatatc cagactagac tagctgtgat
     5941 aaaagaaatg cagggaactg aagataagta aaaagagccc gacggctctt ttttaaaacg
     6001 taaacaagtt atagtgaatt gaatttagaa tagcacatca tagtttctaa agcattgtta
     6061 gagattactt taaaatcctc ttatc
//
LOCUS       DAVMEF010000116         6071 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMEF010000116 DAVMEF010000000
VERSION     DAVMEF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.116"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(94..1284)
                     /locus_tag="VYQ30_001584"
     CDS             complement(94..1284)
                     /locus_tag="VYQ30_001584"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1966663.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(1499..2842)
                     /locus_tag="VYQ30_001585"
     CDS             complement(1499..2842)
                     /locus_tag="VYQ30_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1966664.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(2982..3797)
                     /locus_tag="VYQ30_001586"
     CDS             complement(2982..3797)
                     /locus_tag="VYQ30_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1966665.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(3849..6029)
                     /locus_tag="VYQ30_001587"
     CDS             complement(3849..6029)
                     /locus_tag="VYQ30_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1966666.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT         1792 a         1357 c         1117 g         1805 t
ORIGIN      
        1 gtaaatctct actttcgcaa aatggatttt cagaatagta tcccagtata gttaaaaact
       61 ataaaaaaga gaggacgttc ccctcttaaa atcttatttt actgcttctt tcaaagcatc
      121 taccttatcc aaacgttccc atggaagatc aatatctgta cgtcccatgt gaccgtaagc
      181 cgatgtttga cggtaaattg gacgcttaag gtccagcatt tggataatcc ctgcagggcg
      241 aaggtcaaag atttgacgag ccgctttttc aagttgactt tcagctactg ttcctgtacc
      301 gaaagtatcg atacgaacag aaacaggttg cgcaacaccg atagcatagg ccaactgcac
      361 ttctgccttc ttagcaaggc ctgctgcaac gatattcttg gcaatatagc gagccgcata
      421 agaagctgaa cgatccacct tagtcgcatc tttaccagag aaggcaccac caccatgacg
      481 agagtagcca ccataagtat ctacaataat cttacgacca gtcaaacctg agtccccttg
      541 aggaccaccg attacaaaac gacctgtcgg attgataaag aattttgtct tatcatcaag
      601 ataagaagat ggaataactt ctttgatgac cttgtcaatc acatcttgat ggatttgttc
      661 attagtggcc tctggatcat gctgagtaga aataacgact gtatctacac gtaccggacg
      721 gtcattttca tcgtactcaa ctgtaacttg tgattttgca tctggacgga gatagctaat
      781 ttctccagac ttacgaagtt ctgccagacg acgaaccaat ttatgactga gtgcaattgg
      841 caatggcatg agctcttctg tttcatctac tgcaaatcca aacatgagcc cttggtcacc
      901 tgctccaatc aagtccagtg gatcttgatc agcatttcca cgaacctcca aggcttcgtt
      961 aacaccttga gcgatgtcag gagattgttc caccaaagat gggtgtactc ccaccgtctc
     1021 agcagaaaat ccatattctg tattggtata accaatctct gcaatggtat cacgaaccac
     1081 acggttaata tccacatagg catttgtaga aatttcacca aaaacgtgga cagaaccagt
     1141 atatacagct gtttcagcag caacgtgcgc ctctggatcc tttgctaaaa tagcatccaa
     1201 aatcgcatct gaaatttggt ctgcaatctt atccggatgc ccctcagata cagattcaga
     1261 cgtgaataat ttacgttctg acataaaaat gtcccccctt aaaaaatatt gttatagagt
     1321 tacaaagtac tgataggaaa actcttcgta ataaatacct accatcttat cgggactata
     1381 taactttatc attataccat ttttaatcgc aatttgcagt ctttaatgta gtgtttcgat
     1441 ttagatagtt cgttctcata aataaaaaag ttggagctca gtactccaac aatttctatt
     1501 aaaatgtttg gcgtttagct ttacgctctg cacgaccgcg agcgcgattt tcagcacgct
     1561 tggttttacg gcgcttttca tcaaccgccc attgaatttt cttcttataa cccggtttga
     1621 cttttttctt tttcttttta accaaaccaa tcatttcgat atcaagttta tcttgttttt
     1681 tctcacggtt ggcacgacga tcacggtcat aggtatcttg aaattccccg tctttgacca
     1741 tcttaggact aaacttgatt cccaatttct ccaactcacg gatatccgag tcatcacttg
     1801 gctgataaag ggtaatagct gtacctggta ggccatttcg tccagtacga ccaacacggt
     1861 gaacaaaaaa agataagtct tgcggaatgg catcattgat gacatggctg acaccttcaa
     1921 tgtcaatccc acgtgctgcc aaatctgttg cgacaatata ctcaaaatcc agattttgca
     1981 cctgattcat gattcgcttg cgttcacgag gggcaatatc gccatggatt tttgcaacct
     2041 tcaagccttg agcagtcaga tatgaatgca attcatcagc acgcgtttta gtgttaacaa
     2101 aaatcattgc caaatacggc tgcatcaact gagtcaactg gtaaatttga gcattcttat
     2161 catgtccctt ggtcgaaatc aaccaattat caatggtgtc agaaataacc gttttggtct
     2221 taattttctc cataacagga tttgataagt attttttcaa gaatggttgc agtttttgtg
     2281 ggatagtcgc tgagaagacc atgaattgca agtcttttgg aagactgcca gcaatcttat
     2341 caacagtttc caagaatccc atatccaagg tcatatctgc ttcatcaaca acaaatgtct
     2401 tggctttatg aatagctaaa tcaccagatt taaccaagtc gtagatacgg cctggtgttc
     2461 caataacaat atgaggctga ttgctttcca atttctcaat ctggcgagcc ttatccgtac
     2521 cacccacata attaaccaca cgaacttcga catctgagtg agctgaaatc tgacgcgcta
     2581 cttggtaaat ttgagtagcc aactcacgac tcggtgcagt aatcactgct tgtacactat
     2641 cgctagcttc atctaattgc tggaaaatcg gtaacaagaa agtatgagtc ttacctgaac
     2701 ctgtttttga ttctcctact aggtcacgac ctgccaaaac aataggaatc aacttgtctt
     2761 gcacctctgt tggagttgta aattttaact cctccaaggc ttctctaata tagtttttaa
     2821 attgaaattt cgtaaatgac ataacatcct cgattctatc tatcttatca attataccat
     2881 attttattcc attacagtag tctcacttat ttaggctatt tccagtagct tctctagtaa
     2941 gaaaaggctg gaattttata gttccaacct cttttcagtt attatttcca gtttaacata
     3001 gcattcaagc catagtgatc actcacttgt ggactcttgt taccatcaaa tacgacatgt
     3061 aaattttcca ccgctaactc tttggtagta aagacataat cgattcgaag gggttcagtg
     3121 ttccctttcc agccatcaat ttcaggcgga acagtatagc taccactttt ctcttgagca
     3181 acttcaaatg cgtcttgtaa gcctaatgga ctagctaaaa tagcttggta accttcctga
     3241 cctgctgggt tgttaaaatc tccagctagt aaaagtggct tgttcaattt tttcaagaca
     3301 gcctcaaatc gtgcccattc ttcttggaaa cctttatccc accaagagag atgaacactg
     3361 gcaactgcta gctccttgcc atcgactaca gtttcagcta gggcaacacg gcgagtatga
     3421 tagtctgttg gatcatccac atctgaaacc aaaatttctc tggcttcaat aggtgtttta
     3481 gacaagatag ccacaccttc gtggtagcgg tcatagccga tatggttata agcccaggtc
     3541 cagtagtaat ttttcccttg ctctaacaac ttttcaacca agagtctaac ataatggtct
     3601 tggtgaatag gctcagctgc tggcaaagct tgataaaggt cattaacctc cacctctgac
     3661 gaggtcatct cctgattgat ttcttggaaa caaatcaaat catagtcctt ttcaagaata
     3721 tcttcaagca aaatctggaa tttttcctct gcttctttct ccatccaact gtgagtattg
     3781 agtgttaaaa atttcattct tttctcccaa aataagaggt tggaatacag cttccaacct
     3841 caagtatatt acaattctac tttagcaact gctgttttag ctgcaagaga acctgttttt
     3901 tctaatttaa ctgatttaat tgcatcacca tttgtgaaga caactactgt tgaagtttca
     3961 cgtcctgctg cacggatagc atccaagtca gctgtgacaa ggagatctcc tgctgcaact
     4021 ttttgtcctt cagcaacatg aactgtaaat ggtttacctt caagacttac tgtgtccaaa
     4081 ccaatgtgaa ccaatacttc aagacctgct tccgtcacaa taccaaaagc atgttttgtt
     4141 gggaagatgc ttgacacagt acctgaaact ggagatacaa tgtttccatt tgcaggttct
     4201 actgcaaatc catcacccat cattttttga gcaaatactg gatcctttac ttgttccaaa
     4261 ccaacaactt gaccgtctgc tactgagtaa acttcctcag taagatcttt gaagtgaaca
     4321 gtgttttgtt gtgcttcagt catttggctt ggaagagttt caggaatgat ttcacctgaa
     4381 tcaaggatat cttggatatc agatttcaaa atgtcagctt ttggaccgta gatagcttga
     4441 accccttgtc ctttcatcac aagacccata gctccttctg ctttccattg ctctgcattt
     4501 cctactttat ctgcatcttt aacagttaca cgaagacgag tcatacatgc atcaacatca
     4561 acgatgttta cacgtccacc aagaaggttg ataatgttta cagcttgaga gcctgctgca
     4621 actttcactt cgctgctggt ttcttctgaa ccttcagcag tttcgtagtt tccgttgcgc
     4681 cctggagttg cgtagttgaa tttttgaatc atgaagtttg cgataaagta catgattaca
     4741 gcaaagagaa cagttaccca aacgaagtta acgatatcca taccaatacc agcactgatt
     4801 gcaataggtg tacgagtcaa gaactcgatt gaaccgaatg agtgcatacg taggtttacg
     4861 acgtcagcca tagcgaaggc agcaccttga acaagtgagt aaacaagata cataggtgtt
     4921 gcgatgaaca tgaacatgta ttcgattggt tcagtaaccc ctgtcaagaa tgttgcaaga
     4981 gctgttgcaa tcatcatacc tttgtattta tgtttcttgt cagcatcaac attacggtag
     5041 atagcaacaa tcacacccat caagataccg aatgaaccga tcatttgtcc aactttgaaa
     5101 cgagctggat gtactgtatc taacaagtgt tgatattgac tagcatcagt acctttaagg
     5161 tttacaaggt ctgttaccca tgcaagccat agtgggtctt gaccgaatac ttgagtacct
     5221 ttagctgcac cagttaaaat gtcataagta ccaccaagag ctgtgtagtt cattgggata
     5281 gtcaacatgt ggtgaagacc aaatggcaag agcaaacgtt ccaaagtacc atacaagaat
     5341 ggtgcaagaa ttggagcagt ttcttgtgag ttggcaatcc agataccgaa gttattgata
     5401 cctgtttgaa ctactggcca gaaagcagca agtagaattg cagcgattgc tgaacgaaga
     5461 ataactacaa atggtacgaa acgtttcccg ttgaagaatg aaagtgcatc aggaagttta
     5521 cggaagttgt agtatttgtt gtaagcagtt gcccctacaa aacctgagat aatccctacg
     5581 aataccccca tgttcaaggc tggagcttca agaacactga taaagtaatc agcaactttg
     5641 attgaaccac caaagaaagt agttaccata gcatctggat ttttcaacat atcgcctgat
     5701 acaccaaaaa ttgtaccagt gatacggtta atcaagatga aggcaagacc agcggcgaaa
     5761 gcaccaccag cacgttcttt agcccagctt cctccaatgg ctagggcaaa caaaatgtga
     5821 aggttaccga taacccccca accgatttgc tcaagaattc cacctgtgat gacaagtggt
     5881 gtaaaggttg ggttaatcat cacgatagac ttaccgattg aaatcatcaa accagcagcc
     5941 ggcataaccg cgataactac catcaaagcc ttaccgaatt tttgccaaaa ttcgaaagac
     6001 aagacatttt tgaatgtatc tttcatcatt caaatctcct ttatttttat ttaggcaaac
     6061 gttttacgaa a
//
LOCUS       DAVMEF010000117         6027 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMEF010000117 DAVMEF010000000
VERSION     DAVMEF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.117"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            323..1840
                     /gene="malQ"
                     /locus_tag="VYQ30_001588"
     CDS             323..1840
                     /gene="malQ"
                     /locus_tag="VYQ30_001588"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1966667.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            1866..4124
                     /locus_tag="VYQ30_001589"
     CDS             1866..4124
                     /locus_tag="VYQ30_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEV1966668.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLENIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(5169..5741)
                     /locus_tag="VYQ30_001590"
     CDS             complement(5169..5741)
                     /locus_tag="VYQ30_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966669.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINL"
BASE COUNT         1814 a         1163 c         1174 g         1876 t
ORIGIN      
        1 aaagagggaa atcaggaggg gctacctccc tgtttctaat ccctatctat tttctataga
       61 cgtttgtgct ttgttgcata taaattcaag ctccctaagg aggttttgta ctttgtagac
      121 tgtctcgtga gagtcaaact ttaggattag atagttttaa gttatctttg ctaaaatgag
      181 ttagtctcct taaaaataat caaaaaagtt ttttaaaaac gcttgcaatt atgcttgaaa
      241 aggagtatac ttataagtaa cgcaaacgtt tgcgtctgca aaatacgcaa cgttccatta
      301 ttttaacaca cgaggtgcta ttatgaaaaa acgtcaaagt ggtgtgttga tgcacatctc
      361 ttctcttcca ggagcttacg gaatcggatc atttggtcaa agtgcttacg acttcgttga
      421 tttcttggtc cgtacaaaac aacgttactg gcaaatcctt ccattaggag caactagtta
      481 cggggattct ccttaccaat ctttctcagc cttcgcagga aacactcatt ttatcgattt
      541 agatatcttg gtggagcaag gtttgttgga agcaagtgac cttgaaggag ttgactttgg
      601 tagcgatgcg tctgaagttg actatgctaa aatctactat gcacgtcgtc ctcttttaga
      661 aaaagcggtg aagcgtttct ttgaagtcgg agatgttaaa gattttgaga aatttgctca
      721 agacaaccaa tcatggcttg agctctttgc tgagtatatg gctatcaaag agcattttga
      781 caatcttgct tggactgaat ggccagatgc agatgctcgt gctcgtaaag cttcagcact
      841 tgaaagctat cgtgagcaat tggcagacaa gttggtttac caccgtgtga ctcaatactt
      901 cttcttccaa caatggttga aattgaaagc ttacgctaac gacaaccaca ttgaaatcgt
      961 tggggacatg ccaatctacg tagcggaaga ttcaagtgat atgtgggcaa atccacatct
     1021 cttcaaaaca gatgtcaatg gtaaggctac ttgtatcgca ggatgcccac cagatgagtt
     1081 ttctgtaact ggtcagcttt ggggtaaccc aatctatgac tgggaagcaa tggacaaaga
     1141 cggctacaaa tggtggattg aacgcttgcg tgaaagcttc aaaatctacg atatcgttcg
     1201 tatcgaccac ttccgtggct tcgaatctta ttgggaaatc cctgctggtt ccgatacagc
     1261 agcacctggt gagtgggtga aaggtccagg ctacaagctt tttgcagccg ttaaggaaga
     1321 acttggtgag ctaaacatca tcgcagaaga ccttggcttc atgacagatg aagtgatcga
     1381 attacgtgaa cgtactggct tcccaggaat gaagattctt caatttgcct tcaacccaga
     1441 agacgaaagc attgatagcc cacacttggc acctgctaac tcagttatgt acacaggaac
     1501 acacgataac aatacggttc ttggttggta ccgtaatgag attgatgatg cgactcgtga
     1561 gtacatggct cgttacacga accgtaaaga atacgaaaca gtggtacacg ctatgcttcg
     1621 tacagtattt tcatcagtta gctttatggc aattgcaact atgcaagatt tactagaatt
     1681 ggatgaggca gctcgtatga acttcccatc tacccttggt ggaaactggt cttggcgtat
     1741 gactgaagat caattgacac cagctgtcga ggaaggtttg cttgacttga caacaattta
     1801 tcgccgaatt aatgaaaatt tggtagattt aaagaaataa gacaataatc aggagacaac
     1861 taaacatgtt atcactacaa gaatttgtac aaaatcgtta caataaaacc attgcagaat
     1921 gtagcaatga agagctttac cttgctcttc ttaactacag caagcttgca agcagccaaa
     1981 aaccagtcaa cactggtaag aaaaaagttt actacatctc agctgagttc ttgattggta
     2041 aactcttgtc aaacaacttg attaaccttg gtctttacga cgatgttaaa aaagaacttg
     2101 cagctgcagg taaagacttg atcgaagttg aagaagttga attggaacca tctcttggta
     2161 atggtggttt gggacgtttg gctgcctgct ttatcgactc aattgctact cttggtttga
     2221 atggtgacgg tgttggtctt aactaccact ttggtctttt ccaacaagtt cttaaaaaca
     2281 accaacaaga aacaattcca aatgcatggt tgacagagca aaactggttg gttcgctcaa
     2341 gccgtagcta ccaagtacca tttgcagact ttactttgac atcaactctt tacgatattg
     2401 atgttactgg ttatgaaaca gcgactaaaa accgcttgcg tttgtttgac ttggattcag
     2461 ttgattcttc tattattaaa gatggtatca actttgacaa gacagatatc gctcgcaact
     2521 taactctctt cctttaccca gatgatagtg accgtcaagg tgaattgctc cgtatcttcc
     2581 aacaatactt catggtttca aacggtgcgc aattgatcat cgacgaagca atcgaaaaag
     2641 gaagcaactt gcatgacctt gctgactacg cagttgtcca aatcaacgat actcacccat
     2701 caatggtgat tcctgaattg attcgtcttt tgactgcacg tggtatcgag cttgacgaag
     2761 caatctcaat tgttcgtagc atgactgcct acactaacca cacaatcctt gctgaggcgc
     2821 ttgaaaaatg gcctcttgaa ttcttgcaag aagtggttcc tcacttggta ccaatcatcg
     2881 aagaattgga ccgtcgtgtg aaggcagagt acaaagatcc agctgttcaa atcatcgatg
     2941 agagcggacg tgttcacatg gctcacatgg atatccacta cggatacagt gttaacgggg
     3001 ttgcagcact ccatactgaa atcttgaaaa attctgagtt gaaagccttc tacgaccttt
     3061 acccagaaaa gttcaacaac aaaacaaacg gtatcacttt ccgtcgttgg cttatgcatg
     3121 ctaacccaag attgtctcac tacttggatg agattcttgg agatggttgg caccatgaag
     3181 cagatgagct tgaaaaactg ttgtcttatg aagacaaagc agctgtcaaa gaaaaattgg
     3241 aaaacatcaa ggctcacaac aaacgtaaat tggctcgtca cttgaaagaa caccaaggtg
     3301 tggaaatcaa tccaaattct atctttgata tccaaatcaa acgtcttcac gagtacaaac
     3361 gccaacaaat gaacgctttg tacgtgatcc acaaatacct tgacatcaaa gctggtaaca
     3421 tccctgctcg tccaatcaca atcttctttg gtggtaaagc agctccagcc tacacaatcg
     3481 ctcaagacat tatccattta atcctttgca tgtcagaagt tattgctaac gatccagcag
     3541 tagctccaca cttgcaagta gttatggttg aaaattacaa cgttactgca gcaagtttcc
     3601 ttatcccagc atgtgatatc tcagaacaaa tctcacttgc ttctaaagaa gcttcaggta
     3661 ctggtaacat gaaattcatg ttgaacggag ctttgactct tggtactatg gacggagcta
     3721 acgtggaaat cgctgagttg gttggtgacg aaaacatcta catctttggt gaagattcag
     3781 aaactgttat cgacctttac gcaaaagcag catataaatc aagcgaattc tacgctcgtg
     3841 aagctatcaa accattggtt gacttcattg ttagcgatgc agttcttgca gctggaaaca
     3901 aagagcgctt ggaacgtctt tacaatgaat tgatcaacaa agactggttc atgactcttc
     3961 ttgacttgga agactacatc aaagtcaaag agcaaatgct tgctgactac gaagaccgtg
     4021 acgcatggtt ggataaagtc atcgttaaca tttctaaagc aggattcttc tcatctgacc
     4081 gtacaatcgc tcagtataac gaagatatct ggcacttgaa ctaatactct tcgaaaatct
     4141 cttcaaacca cgtcagcttt atctgcaacc tcaaagcggt gctttgagca acctgcggct
     4201 agcttcctag tttgctcttt gattttcatt gagtataaga tacaaattta tactaataca
     4261 ttttgtaaaa aagcgagttt cgattgaaat tcgctttttt aaatgctgtg gatttgggtc
     4321 aatcttgtct aaaaataggg aaatcctaga tacagtgaag gctttaaatg ctggttttta
     4381 ctgtcttcag ccttatattt tttcgtagtt ggttacctca tatctcttgt attcgcttac
     4441 ataaagtatt ataatataat tgtaggaaag aaggtgtttt tatgatatac acacttaaat
     4501 tggtgttgtt tattaccttt cttgtaataa gcttgttacc tgataagatt tttggaaaaa
     4561 ataaaaaaat ttggaaaata gtttttgcaa tattgacggc agtggcagca ttgtcattta
     4621 tgtactaagt tattttaaga atgtagggaa ataaacccta cattcttttt agttttttct
     4681 gttttctaaa ttctatttat ccaagcgctt caacatttct tgcttcttcg cttcaagttc
     4741 tgcacgcttt tcttcgattt cggcatgttt tttctcgagt tcagaacaac ttgcaccatt
     4801 gctaaattct tttcgccatc aggagatagg gtgagtcgac atgtctatta ctcacccaaa
     4861 gcagtcctac aaagcaggaa ttttctgtta cttttttgga aatagtaacg tttatacagc
     4921 tttgacactt cgtatcaaag cgccaaacac actccgaggg atttacagaa agcagaaaag
     4981 gaatgatctg gtataagatc attccttttc tctctttttc tttaagtaat tatatacaat
     5041 gtacgacgaa gtcgtcattg caatgctgat ccaccaccta aagggaactt taaacaacat
     5101 tgataagata aagaatataa acaacgaaaa tacgttatac ccaattaatt ttattgtata
     5161 tctcatgatt aaaggttaat ccttccgttg ttaggaatgg catcattttt atcccataat
     5221 tgtgctaaat aagtccccgg tgataataaa ttcatagcga attctaaagc aacatcattt
     5281 acaaaccaac tacctagata tctagaaatt gctgaacgaa tagcactttt tgctgcatgt
     5341 tttcctttta ctttaattag atttgcaagg cctgcagtag ttcctcctaa tgctaaagct
     5401 attgcagtat ctaatagagc acccatttga ttaactgtaa taccttgcca agctgctcta
     5461 aatggagagt atgtaggtgg gattgtataa tcgccttgta attgtcggtt aattacttct
     5521 ttgatccatt gttgtgagac gtctggatga aaagattgga tttcgtttgc aagtgtattg
     5581 atttgttctt ctgttagaga agtgacaggt tgaagttcca tatttgtttc aatttgtgat
     5641 acttgttcag aagcgtatac agctgaaaca cttggaatcg ctgatacaat taacacaatt
     5701 gacgtcaaaa aaaccgaaat aaatttcatt aatttgttta tgagcttttc tcctttttat
     5761 ttgtatctgc ttacatttta ttatatactg ttattatagt caagaaaaat atgctattat
     5821 gttaaaaaaa tatttttcaa aatataaatg gacggattta ttttggattt tatttgttat
     5881 tttgacctgc ctctatattg gtaaccatga tttgtttact ctcaatcatc aagaattctc
     5941 ttttcgtggt agcgtttggg gtctggtact ggccttatat cacttattgt tcattgataa
     6001 gtttgtaatt tcgaatcgaa aataaag
//
LOCUS       DAVMEF010000118         5929 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMEF010000118 DAVMEF010000000
VERSION     DAVMEF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.118"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(101..505)
                     /locus_tag="VYQ30_001591"
     CDS             complement(101..505)
                     /locus_tag="VYQ30_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1966670.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEDRQVRI"
     gene            complement(575..2056)
                     /locus_tag="VYQ30_001592"
     CDS             complement(575..2056)
                     /locus_tag="VYQ30_001592"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1966671.1"
                     /translation="MILVDKFVTHVIFESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDETPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQIGILLD"
     gene            complement(2075..3253)
                     /locus_tag="VYQ30_001593"
     CDS             complement(2075..3253)
                     /locus_tag="VYQ30_001593"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1966672.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEVDQTFFSPGRINLIGEHTDYNGG
                     HVFPVAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            3417..4424
                     /gene="galR"
                     /locus_tag="VYQ30_001594"
     CDS             3417..4424
                     /gene="galR"
                     /locus_tag="VYQ30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1966673.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEKIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(4470..5507)
                     /locus_tag="VYQ30_001595"
     CDS             complement(4470..5507)
                     /locus_tag="VYQ30_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1966674.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDVCLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(5508..5861)
                     /locus_tag="VYQ30_001596"
     CDS             complement(5508..5861)
                     /locus_tag="VYQ30_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1966675.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREEKLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
BASE COUNT         1705 a         1458 c         1122 g         1644 t
ORIGIN      
        1 ctaaattttc tattttagaa ttaaatcata tcaatatcaa aaaatgagta aggcaaatct
       61 tataaattcc aaaattcacc tcactctatt aaattactct ttatatcctt acctgtctgt
      121 cttctgaccg ctggcctgtg acaaacatgg taaaggttgc tttgcagaca tttctgcctt
      181 cttgattggt gatatccaca tccattacac aggttgtacg accttgatgg acacattctc
      241 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
      301 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagcg
      361 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
      421 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
      481 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaacga cactatgata
      541 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtaaaatt ccgatctgtt
      601 ccacaaagcg cataaaggct gtctgacctt gttctgtctg cttgtagact cctgcatcct
      661 caagtacacg cacaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
      721 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
      781 caactgtaac agcttctcct acaagatagc tagcgacttg ctccacttct tctttcagac
      841 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
      901 gttggacatc cttgtgggga tgatagatgc catcaggatg ctctgctgaa gtctgattgt
      961 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
     1021 tgtgatgcgg tgtctcgtct gtctctgcta aaatctgcac actagaatct gaatactgac
     1081 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
     1141 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
     1201 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
     1261 aatgatcatg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
     1321 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
     1381 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
     1441 aatactggaa accccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
     1501 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
     1561 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
     1621 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taagcgatat
     1681 ttttagcaat ggccttgagt ttgatgtagt catttttccg actgagttga taaaaatcct
     1741 ctatcgcttg ctcaggagag taggcatagg cttcccagaa gtcacgattg acctgactcg
     1801 gacaaggagt caccaagtcc atcagttcag taccaaggat ttcacgcgca gtctgactat
     1861 cctcaatcgt ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
     1921 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacggttgg
     1981 tcaggtagat tcgatccatt tcctcaaatg aactttcaaa aatgacatgt gttacaaatt
     2041 tatctactaa gatcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
     2101 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
     2161 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
     2221 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
     2281 gggtatccaa ttccaaacca gttacttcat aatcatgctc cagagaaacg tgtgacgcat
     2341 tcatcaagcg tccaaatgtt tccaaatctc ctgcttggag tgctacttga gctttgaggg
     2401 tacgttggtt ttcaagcaca gcatggcgag cacgtttcaa acgattttca tctttaatca
     2461 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagagtctga atatccaagg
     2521 aaacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
     2581 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
     2641 gtggcaccaa gtcgtattct aaagtattag tatctaggta aatagcacgt tggtctgccc
     2701 ccataccaat agcaaactgg tccataatgc cagagtttac tccgataaag ttgttttctg
     2761 tttgtttgcc gattttaacc aaatcgagac gctctaattt taaatcaaag agatgctcag
     2821 ccacgactcc tgtcaagagt tccaaggatg cagaagaaga caagccagca ccatttggaa
     2881 tatttccata aacataaaaa tcaaaacctt tgtcaatgac atgcccagct tcttgcaaga
     2941 aatggagaac cccttttgga tagttggtcc agttgtgctc tttttcaaac ttgaggtcag
     3001 caagaggcac ttcgataatg cccttgtcct caaagttggc tgagtagaaa cgcaagactt
     3061 ggtcgtcacg cttgcgagct gcgccgtatg ttcccaagga aatagcaact ggaaatacat
     3121 gtccaccatt atagtccgtg tgttcaccaa tcaaattgat acgacctggt gaaaagaatg
     3181 tttggtctac ttcttgacca aaaacagcaa gaaagtcttt gcgaagagtt tcagcagtaa
     3241 gatgttgtgt catatgattt ctcctttgac tgtccgttaa gccaccttaa cgtgtaatcg
     3301 ttttcttcta ttgtatccaa gggatttccc aaggtcaatc ctttttacta aaattttact
     3361 aaactatcag taaaaagcag aaaaatatga tatactaacc taatcaagga ggatttatgg
     3421 ctaccttaaa agacattgca cagctagcct ctgtctctat cgcgaccgta tcccgcgtcc
     3481 tcaaccgcga ccagagccta tctgttacag aagaaaccag acaccgtatt ttaaccgttg
     3541 ctgaagagct gggctacacc aagcacctca agacaggcga gtcccataaa cccaagcaaa
     3601 agattgccat tattcaatgg gtcagcgaac aaggggagct agacgacctc tactactacc
     3661 agattcgcct aggaatagaa aaaagagccc aagagttgga ctacgatatc ttgcgctatt
     3721 ttaatgacca cccttttacc ctaagcgagg aagtgattgg gattctctgc atcggaaagt
     3781 ttagtcgagc tcagatttct gcctttgaag aataccaaaa gcctcttgta tttctagaca
     3841 gcgatacact ttccctggga catacctgta ttatcacgga tttttacact gctatgaaac
     3901 aggttgtcga ttatttcctc agtcaaggga tggaccgcat cgggattcta acagggcttg
     3961 aggaaacaac agaccaagaa aaaatcattc aggacaagcg tctagaaaac ttcaaaaact
     4021 acagtcaagc gaggggaatc tatcatgatg aactggtctt tcaaggaaga tttactgccc
     4081 agtctggcta tgacttaatg aaggaggcca ttcagagctt gggagaccaa cttccgccag
     4141 catttttcgc agccagcgat agtttagcta tcggtgccct ccgtgcgctc caagaagctg
     4201 gaatcagcct gccagatcgc gtcagcctca tttcattcaa cgacaccagt ctgaccaaac
     4261 aggtctatcc tcccctctct agtattacag tttatactga agaaatgggg cgagcaggta
     4321 tggatattct taacaaggaa gtcctccacg gtcgcaaaat tcctagcctg accatgctgg
     4381 gaaccagact gacattaaga gaaagtaccc taaatcaaga ataggataac ataaaaaacg
     4441 aatagagttc taaaactcct attcgttttt tattcgatta caatcataga cttaattgtc
     4501 ttacgttcat ccatatcttt gtaggcttgg tcgatatctt ccagtttata acttgaagta
     4561 aagacgcgac ctggattgat atcaccatca aggacggctt ttagtaaaaa ttgcttatcg
     4621 tatgttgtag cagaagctgc cccacctgct acagagatat tttgcataaa tgtcgaacca
     4681 agagcacgat tattatagtg tgggactcct acaaagccca tacgccctcc attatgaaga
     4741 acacctagcg cctgttctat agcagcctcc gtaccaacac attcaagtgc tgcatctgct
     4801 cctccaccga ggatttcacg caccttggta attccttctt gaccacgttc tgcaacaaca
     4861 gctgtcgcac ctgactccat agccatcttt tgacggtctt catgacggct cataaggata
     4921 atttgtgatg ctccacgcat cttagccgcg atgacagcac attgaccaac agccccatca
     4981 ccgataacaa caaccttgtc ccctttttga acatttgcaa cacgcgccgc atgatagcct
     5041 gtcggcatga catctgcaag agtcaaaagg gacttgagca tcccttctgt atagtcagaa
     5101 ggttgaccag ggattttaac cagcgcccag tttgcatagt ggaagcgaat atattctgcc
     5161 tgaaaatcac cccccaaatt attgccaata tgattgtcgc aagaaccgtc aaatccagca
     5221 agacagacat cacactcacc acatccatgt gtaaaaggga caatcacaaa atcacctggt
     5281 ttcaccgtcg taatggcttc cccagcttct tcaacaatcc caatcgcttc gtgtccacta
     5341 tttttatgtc cagctttcgt ttctggatta cggtacctcc ataaatctga accacaaacg
     5401 cacgcacgaa ccacacgaat aatcacatca tccgcttcta ttatttgcgg acgttcaatg
     5461 ctagcaagtc caacctgacc tgcctttgta tatactgctg atttcattta aaattttcct
     5521 tccttataaa gtttaatttt gagatttaaa cgatttaaag ctgtctgtag ctgagacaag
     5581 cgctcctcta atttttgctt ttcctcttct aaaataccaa gcttctcctc tctcgtttca
     5641 tctccctttt ggtagagcga catatagtca actaaactat ctacagagac acccgccgaa
     5701 cgaaaacact taataaattc cagcgcttcg atatcctgat cttgaaaatc acgaatccca
     5761 gtagcagtac gagtaatcgg tggcacaaga ccaacccgtt cataataccg aatcgtatcc
     5821 gctgaaattc ccaacaaatc actggcagat ttaatattca cacgctaacc tcctctcatt
     5881 tcattcactg aattcacgct attctaactc acttatctta ttatataac
//
LOCUS       DAVMEF010000119         5900 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMEF010000119 DAVMEF010000000
VERSION     DAVMEF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.119"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..628
                     /gene="deoC"
                     /locus_tag="VYQ30_001597"
     CDS             <1..628
                     /gene="deoC"
                     /locus_tag="VYQ30_001597"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1966676.1"
                     /translation="KQDAKKKQIDSLLSEAREYGFASVCVNPTWVEHAKKGLEGTDVK
                     VCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNLALVESDIRAVVE
                     ASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGGATIADVTLMRET
                     VGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADGDY"
     gene            615..1004
                     /locus_tag="VYQ30_001598"
     CDS             615..1004
                     /locus_tag="VYQ30_001598"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1966677.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            1094..2146
                     /locus_tag="VYQ30_001599"
     CDS             1094..2146
                     /locus_tag="VYQ30_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1966678.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            2290..3825
                     /locus_tag="VYQ30_001600"
     CDS             2290..3825
                     /locus_tag="VYQ30_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966679.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            3818..4876
                     /locus_tag="VYQ30_001601"
     CDS             3818..4876
                     /locus_tag="VYQ30_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966680.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            4879..5835
                     /locus_tag="VYQ30_001602"
     CDS             4879..5835
                     /locus_tag="VYQ30_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966681.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         1634 a         1114 c         1393 g         1759 t
ORIGIN      
        1 aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta gagagtatgg
       61 ctttgccagt gtttgcgtta atccaacctg ggttgaacat gctaaaaaag ggcttgaagg
      121 cacagatgtt aaggtttgca cggtagtagg tttccctttg ggagcaacaa cttcagccgt
      181 gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg atatggtgat
      241 caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata ttcgcgcagt
      301 agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc ttctgacaga
      361 ccaagaaaaa gttgttgttt gccaattggc ccaaaaagct ggggctgact ttgtcaaaac
      421 atctactggc ttttcaactg gtggtgctac gatagcagat gttacattaa tgcgtgaaac
      481 agttggatct gatatgggag tcaaggctgc tggtggagct cgttcttatg cagatgctct
      541 tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag ctattttaaa
      601 aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa accagcaaac
      661 atgcctatgt cccctattct cactttccta tcggagcagt tttagtagcc aaagacggaa
      721 atgtttacac gggagtgaat atcgaaaatg ctagctatcc tttgaccaat tgcggtgaga
      781 gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa ttgattgtct
      841 atggtcaaac tgaaaaaccg atttcaccat gtggtgcttg tcgccaagta atggtcgaat
      901 tttttgaaca agatttaaaa gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga
      961 cggtcgggga attacttcca tactctttta cagacttaaa ctagtctgag tcgctctctg
     1021 agtggcacgg tccttgtggc caaccaatcc atacttgcaa catcgttgca catcttattt
     1081 aggaggttca gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc
     1141 agttggactt gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt
     1201 gaagacaaaa gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca
     1261 atcagcttgg gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg
     1321 tttcacttac ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc
     1381 tggaagttac aacctaatct tcggtgttgg ttttgccctt cataatgcag ttaaagatgc
     1441 agcaaaagaa cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa
     1501 gaatgttgcg agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc
     1561 agcaaaaaca actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat
     1621 ctctcgtttt gaagcaggat tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt
     1681 ccaagttgac tacgctggtt catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc
     1741 acaatacgca gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt
     1801 ctttgcagag gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat
     1861 cggtgttgat cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc
     1921 aaactttgtt cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa
     1981 caaggcagaa aaaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa
     2041 aggggttgac ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc
     2101 aaaagctaaa atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat
     2161 ttcttaggaa gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctca
     2221 gtaaagcttg ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag
     2281 gaagagcaca tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt
     2341 ggatttgttg ctaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt
     2401 ttaggagaaa atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa
     2461 ccaactagtg gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa
     2521 gcagctagct tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca
     2581 gtggctgaaa acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct
     2641 ggagctagca aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct
     2701 gccaaggtag cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt
     2761 tatcgggggg ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa
     2821 attgatgagt tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg
     2881 attacccaca aattggatga aattcgagca gtttctgacc gtgttacagt tatccgtcgt
     2941 gggaaatcaa ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg
     3001 atggtaggac gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg
     3061 gttttgtcta tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat
     3121 ctgtccttgg atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt
     3181 cagtctgaac tgattcaagc cattacaggt cttcgcaagg ttgaatctgg tagcattgag
     3241 ctaaaaggag attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg
     3301 cacgttccag aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat
     3361 attgcccttc aaacctacta taaagaacca catagtaaaa atggaatttt gaattattca
     3421 aatattactt cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa
     3481 ttagttcctg cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa
     3541 attgatcgag atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt
     3601 gccattgagt atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt
     3661 gttgtcagct ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac
     3721 gatggtaaga ttcaaggtat tgtatcacca gaaacaacca ataaacaaga acttggtgtc
     3781 ttgatggctg gtggaaactt gggaaaggag aagagtgatg tttaaaaaat tacaacaaat
     3841 ttcggttccc ttgatttctg tatttctagg aattttactc ggagccattg tcatgtggat
     3901 cttcggttat gatgctattt ggggctacga agaattgttc tatacagcct ttggcagtct
     3961 gcgtgggatt ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt
     4021 tgcggttgcc agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg
     4081 ttggattctc agtggttggt ttgcgctgtc gcatccagat atgccccgtc ccttgatgat
     4141 tctagcaacc atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat
     4201 tcttagggcc tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt
     4261 cttgtatgta gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga
     4321 ttcgaccatt cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac
     4381 tggtaactca cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg
     4441 gttcatgctc aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca
     4501 tgcttcagaa tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc
     4561 aggtgccttg gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta
     4621 tgttcaaggt tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc
     4681 caactcacca attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc
     4741 tcctggtatg aatgcggcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat
     4801 tatcttcttt gtcagtgttc attaccttat cgaacgcttt gttaaaccga aaaaacaagt
     4861 taaaggaggt aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg
     4921 ctgatttact cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt
     4981 ggtgtggtaa acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc
     5041 tttaaccttg aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta
     5101 gcaggattgg ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt
     5161 gcagaccatg ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc
     5221 ttagttaaag ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc
     5281 tttgatttcc cagtcttggc aaatatccca gtgattggtg atatcttctt caagtcaact
     5341 agtctacttg gttatctggc gattgccttc tcattccttg cttggtttat tctctttaag
     5401 actcaatttg gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg
     5461 ggaatcaacg tctacaagat gagatattta gggattatta tttcaggttt tctaggtgga
     5521 attggcggag cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt
     5581 ggacctggat ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct
     5641 atgctttcta gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg
     5701 ccgttcctac aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt
     5761 cttgtcttag cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac
     5821 atcaaatcaa aataagcata caaaaaaacg tcagttcaat gactgaactg ccccccaaaa
     5881 gttagacaga aaaaatctaa
//
LOCUS       DAVMEF010000120         5807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMEF010000120 DAVMEF010000000
VERSION     DAVMEF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.120"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..616
                     /locus_tag="VYQ30_001603"
                     /pseudo
     CDS             1..616
                     /locus_tag="VYQ30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(741..944)
                     /locus_tag="VYQ30_001604"
     CDS             complement(741..944)
                     /locus_tag="VYQ30_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966682.1"
                     /translation="MIKPYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(937..2019)
                     /locus_tag="VYQ30_001605"
     CDS             complement(937..2019)
                     /locus_tag="VYQ30_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1966683.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTGTSKDLLYFD"
     gene            complement(2145..2927)
                     /locus_tag="VYQ30_001606"
     CDS             complement(2145..2927)
                     /locus_tag="VYQ30_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1966684.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2927..4270)
                     /locus_tag="VYQ30_001607"
     CDS             complement(2927..4270)
                     /locus_tag="VYQ30_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1966685.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQIIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4369..5685)
                     /locus_tag="VYQ30_001608"
     CDS             complement(4369..5685)
                     /locus_tag="VYQ30_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1966686.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
BASE COUNT         1691 a         1144 c         1291 g         1681 t
ORIGIN      
        1 agtctgacat gaaggctgga ctaaagatag agtttctctt gaatactgtc tcccaagtcg
       61 tacgatactt cttaactggc tagcacaata caggaaaaac gggtatacta ttgttgagaa
      121 aacaagaggg agagtacctg agagcggaga atgccatcct aaaaaagtta agagaactcc
      181 gattgaagga ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat taatgactga
      241 gttttcgtta gatcttcttc taaaagccat taaactagct cgttcgacct actactatca
      301 cttgaaacag ctagacaaaa cagataagga ccaaggctgg caatatcaac acgattctta
      361 tcatcggttt ctagagagta agggaattca agcatccatg tcacgtaaag gtaacagccc
      421 agacaacggt atgatggagt ccttctttgg cattctgaaa tcggagatgt tttatggtta
      481 tgagaagtca tttcagtcgc ttaagcaatt ggaacaagcc attgtagact atattgatta
      541 ctacaacaac aaacgaatta aggtaaaact aaaaggactt agtcctgtgc agtacagaac
      601 taaatccttc ggataaatta attgtctaac tgtttggggt cagtacacaa tacctgatag
      661 tatgcgtttt attgtgggaa gattttcctc attttctagt gaaattgaac tttttctaac
      721 atcttttgat agatgacttc ttaatcaatt atcatttcca ttgttgtttc ttggggtttc
      781 atcccttgtc tttgataaaa gcggagagca ccctcgttat cattccagac acttagggtt
      841 agattattgc agccaatttc tttagcataa tagcagaaaa atgacaataa aatagcccct
      901 agatattttg aaaggaaagc ataggggcta tatggtttaa tcaaaataaa gcaaatcttt
      961 gctggtgcct gtaaagaggt cgaagccatc cttggtaaca acaccgcagt cttcaatacg
     1021 aacaccgact ttaccaggga tatagatacc tggttcaaca gagaagcaca tgccttcttc
     1081 gatgaccatg tcgtttcctt ccatgataga tgggaattca tggacatcca taccgatacc
     1141 atgcccgaga cggtggttga agtactcacc ataaccagct ttttcgatga cctcacgggc
     1201 agcgcggtct acttcatgag cagtcacacc tggcttgata aagtcaagag cagcttgttg
     1261 ggcttcaaga gtcaagttgt aaatatcttt cttgaattgg tctggtttgc cgacagcgac
     1321 tgtacgagtc atatctgacg catagccatt gaccagaaca cccaggtcaa agaggagaag
     1381 agcatcattt tcaaccttat tagctgctgg aatgccgtgt ggattcgcag cattatcacc
     1441 agtcaagacc atggtatcaa agctcatttc gtaaccttca cgtttcatgg caaagtcaat
     1501 ttgtgcgatg atatctgtct cagtcttatc aagagaaata ttgtcaaaac caacatgaac
     1561 agccttgtca gcataaagac ctgcaaccat cattttttgc acttcatcag ctgatttgat
     1621 gaggcgcatg cgttggatac gaggagtgag gttgtcaaac tcagcagtct caaaaactgt
     1681 tttcaaacca tggtatttgg tcaagatgag attgtcaaac tcaacagcga cacgtttgaa
     1741 gtcaagttgt ggaagagcat gtttgatttt ttgccatgga ttttcagaat cgacatagcc
     1801 cactactggg aaggaaacgg tgctacttgc acgttctact tcaagagctg ggacaaagag
     1861 gagaggttcc tgatccgcta ggacaaagag gaacatttgg cgttcatggg gatcactgta
     1921 aaaaccagtg aggtaattga ttgtgacggg gtcagatacg acagcgacgt ctagtttttc
     1981 tgattcaaga tatgttagga tttgttgtaa tttagacata tgctaccttc tttctacccc
     2041 tctattttgg caaaaaatga gagaaaatgc aagggagaag ggtaaaagaa aagacaaaaa
     2101 actccgacaa gataacggag ttctttgata tcattttcct ttgtttaaga aaagtcagcc
     2161 ttgcttttga cgtcactgta ctcttcagcg atttcttggc tgaggatatc ctcataggca
     2221 gggagttgga tatcctgacc atatttcttc ttgagggcag tagtaactag gcgataagcc
     2281 agattggcat tacgagagag gataggtccg tggaagtagg aaccaaagac attcttataa
     2341 tgaacccctt caccgacctt ttcttcgttg tttccatttc catagacaac ctgtcccagc
     2401 ggtttttggt catcagagag gaaggtacgg ccttggtgat tttcaaatcc atagtaggtt
     2461 tcatcgaaat cttcattgtg aatcttgatg tcaccgataa aacggttatt ggtctggttg
     2521 agcgtgtagt gtcccatgac ccctagccct tcgatacgtt ttcctgaagc ttcaacataa
     2581 tattgaccca atagttggaa accaccgcag atagccagaa ctacaccgtc gttttggatg
     2641 tagttgtcaa tgctctcttt tttagcaggt aggtcgtctg caatgatact ttgttcaaag
     2701 tcttgaccac caccgaaaaa ggcgatgtcg tagtgatttt catcaaagtc atcatggaga
     2761 gaaacgatgt caacggtcac atgggctccc agtttttcag ccacatactt gagcatgagg
     2821 atgtttccat tgtccccgta ggtattcatg agatttccgt agaggtgggc aatgttgagc
     2881 tgataggggt aattgccatc ttttgaggaa agtgaagtat aaaccatcag ttcatctcct
     2941 ttctaacaat ctgacgacta gccagcagtt cacgaaattc cagcatggca gtataagttg
     3001 ccagaatata ggcatgcttg cagtcttgat tctcaatggt cttgagaact tgctccagat
     3061 tactcgtttc agtgattttc tcagctggat agccagtcac tcggaggcga cgagcgattt
     3121 cagaatgacg aacaccgcca gcgttgattt caggaatgtc catgtcagtg atttgctcaa
     3181 agtctgcatc ccagatccag ctagtgtcaa ttccatctgc atagttggca ttaaggagga
     3241 cagataggct aaatggataa ggtgctagtt tgatcatttc gatagcttgg gttgcaccga
     3301 ctggattttt aatcaagaca agggtacatt ccttgtcacc gatatgaaag gtttcttggc
     3361 gtccaaagac agcacggctc ttgtcaaatc cttgtttgat gatttgcgaa tcggcaccta
     3421 ggaaacgggc gatggccaca gcagctaggg cgttatagat attatagagc ccgccgattt
     3481 ggataccgta ttcttggccg tctatgacaa agcgagagcg attgttggtc aactcaacca
     3541 attttgtcaa acgatagtcg agatcaggac gtttacatcc acaaccttca cagatatagg
     3601 cacccaagtt tgcataggta ttatgctcat atttgaggat gccttggcag tcaggacaga
     3661 gaatcccttc ggtattgtag tgagccagtt gggctggtcc tttttccaag tcaaaaccaa
     3721 aatactctat agggtttgga atagttggct tgtagaaaag tggactgtct ccgttaagga
     3781 gaacagtagc agttggaact ttccgaatgg catccaatat catgttatag gtagtataga
     3841 tttcaccgaa acggtccatc tggtcacgga agatattagt aatgacaaaa agactaggct
     3901 ggatatagtc acagatacga gatagactgg cttcgtcaat ttcgaggacg gcaatatttt
     3961 tcccagtttt tgaagatttg gctgttagga aggttgttgc aatccctgta atcatgttgg
     4021 caccgcttgg gttggttaga acttgaccat aaacctcttt taaaatgccg acagtgaggg
     4081 cagttgtcag ggtttttcca tttgttccag tgacaacgac aatctcgtag ttcttagcta
     4141 ggttttgtaa aatatcttta tcaaattgaa gggcgacttt acctgggagc gtacttccac
     4201 ggccaagacg gcttaaaatg aagtgggaag aacgcccagc aagaaggccc aaagtagttt
     4261 ttaagttcat gtttctatta tatcataaaa gaggagaaag acagatttga gattccgcag
     4321 aagtaaaaac agcccaaaga gggctgttcg atacgattaa atctcaactt aaatcgcaaa
     4381 caagtcattc aagttctcag ccaaggttgg gtgagtgaag atttgttttg tgaagtaagt
     4441 gtaaggaatc ttgttgtcca tagcaacagt gatgatgttg atgatttctt gagaaccttc
     4501 tgagaagatg cttgctccaa gaatttcttt tgtttcagta ttgacaacag ctttgaaggc
     4561 accgcgaagg tctccattta cgtgaccacg aggcattgct gcaacgggga tttccttaac
     4621 agcgtatgga agtttcaaat cagctgcttg gctttcagtc aaaccaactt gtgaaagtgc
     4681 aggtgtgatg aacatagtat ttggcacatt gagacggtct tcaagtgtgt agctgccatc
     4741 tccagcaagg tagctgtaaa caacacggaa gtcatcaagt gaaatgtaag taaattgaag
     4801 gccaccgttg acatctccaa ctgcaaagac accaggaacg tttgtttgac agtgtttgtc
     4861 tactttaata gcaccacgtt cagttagttc aatatctgta ttttcaagtt gaagtggttc
     4921 tacatttggt ttgcgtccag ttgcgtagag aagggcgtcg aaacggtaag tttcgtcttc
     4981 agttacgaca agcacttggt caccatcgtt tttgatttca gtagtatgga tattttgaag
     5041 caattcaatg ccatcttctt ccatgtattg tttagcaaga gctgcgatgg aaggttctgc
     5101 acgaggtagg aatgtatcca aggcatctag gactgtgacc ttgcttccaa gtttgttgta
     5161 aaggccggca aattcaagac cgatatttcc gccaccaagg attccaagtt tttcaggtaa
     5221 tttgtccaag ttttggatac ctgttgagtc aaagatgttt ttgcttgtag caagtccagg
     5281 gattggcaag acgtttgaaa cagcaccagt gttgatgacg attgtttcag cagtcagttc
     5341 tttcttttca tcaccagctt ggatttcgat gactttattt gaaaggaagt gagcttccgc
     5401 atcaaagata tctacgcctg taccagcaac agtcgcatag tttttaccgt tgaggcgacc
     5461 agtgatcgtg tttttagtag caatcacttc ttcaaaagac aagtcctttt cagcagcaac
     5521 tagcaaggtt ttagttggga tacaaccaat gttgatacaa gttccaccgt acatagcctt
     5581 gctgcgttca acgagggcaa cttttttgcc agctgaagcc aatttacctg ctagtgtttt
     5641 accagcttta ccaaatccaa taacaattaa atcgtatgtt aacatttatc ttcctcctat
     5701 tcgaattaca ttctagcaca agaaaaaaac ttttgcacaa ttctgctacg gaaaagacag
     5761 aaaaagataa aaacgctttc cagacaaaaa actagtaatt ttttgac
//
LOCUS       DAVMEF010000121         5776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMEF010000121 DAVMEF010000000
VERSION     DAVMEF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.121"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(11..517)
                     /locus_tag="VYQ30_001609"
     CDS             complement(11..517)
                     /locus_tag="VYQ30_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966687.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNVYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKVYQQFVQ
                     LEKNSLSL"
     gene            complement(548..862)
                     /locus_tag="VYQ30_001610"
     CDS             complement(548..862)
                     /locus_tag="VYQ30_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1966688.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(872..2851)
                     /locus_tag="VYQ30_001611"
     CDS             complement(872..2851)
                     /locus_tag="VYQ30_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1966689.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLLKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            complement(2969..3283)
                     /locus_tag="VYQ30_001612"
     CDS             complement(2969..3283)
                     /locus_tag="VYQ30_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1966690.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(3413..3652)
                     /locus_tag="VYQ30_001613"
     CDS             complement(3413..3652)
                     /locus_tag="VYQ30_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV1966691.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(3704..5140)
                     /locus_tag="VYQ30_001614"
     CDS             complement(3704..5140)
                     /locus_tag="VYQ30_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1966692.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(5330..5668)
                     /locus_tag="VYQ30_001615"
     CDS             complement(5330..5668)
                     /locus_tag="VYQ30_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1966693.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT         1915 a         1130 c          723 g         2008 t
ORIGIN      
        1 tagatcagga ttataaacta agtgagttct tttccaattg gacaaattgt tgataaacct
       61 tatctgttcg tttataaatt tttttaattc ttctaatgtc taacaaactc agaactaaac
      121 ctaatagaag aactacaaaa acaaataaac gtgctacttg gttattttca aaaatcggaa
      181 aaagattctt aaaccaactt gtccaagtta aaacaagtaa tcctattgaa ataagcattt
      241 gtattctaac aaacattagt gttattccca acttttcttt cctatttcca tagagtttaa
      301 attgttcaac agttgctaaa atagaaaata ctatgagcat aatggggaaa ataataatag
      361 gcgagggact aataaactga ctcaaaagcc aataaacatt tccaaaaaag aagagtgcta
      421 ttgaataacg tagaagaaga tatcgattaa aaaaagtatt agttagagcc atctctcgac
      481 gttgttgttc aattttttgt cgttcttttt tatccatatc atttcctcct tatataacaa
      541 cacatattta gttaactttc ttataaagag ctaacatttc ctttgctact tctaataatg
      601 tcatagtggt cattaaatga tcttgagcat gtaccatgat aatttcaatt ttaatttcca
      661 ctccacttgc gtattcttgc aagagtttgg tttgtgcatg atgcgcttca agaattatct
      721 catttgattg atttaattta ctttctgcat catcaaaact accttctctc atttttgcaa
      781 atgcttcatg tatttctgac cttgcatttc ctgaatgcag gataatttca aatgctgcaa
      841 cctgcagttc ctcttgattc atatgaacct cctattttat cttctcaaat atgttaataa
      901 aatcttcaaa gttattgcaa gatattagct gattttgcaa ttcatcattc tctgtcagag
      961 agactatctt tttagtcaca gttgccaaac cttcgttccc atatattgat ggagatagaa
     1021 gaaatactag ctggacatgt gaactttgat tatcccagag taacgaatct ttacaaattg
     1081 caaccgaaac ctttccctct gtaccaaagg gctgaatagg atgcggaact gcaatttttt
     1141 cagaaaaaac aactgaactt aattcttcgc gctgtttaat tccataaagt aaagattgtt
     1201 caaactcatt tgattcacca acagataaac cctcaaccat cttttcaagt aaatttacct
     1261 tgtctgattc agtacatatt aaaaaatttt ctttactaaa atactgtcta aagccgttgt
     1321 tttcaaattt gttaatcttt gatgattgta cataactaga aacttgcatc tgatccatag
     1381 cttttctaat catttccatc tcatcacttt taagaaacac actaacttta aaaactggga
     1441 tttgaaaata tagatttgat aaatcaacag ctgacactat aaaatctatt cctttaagtt
     1501 tttcttgatt caattcatag tagcctatta catcaacaac ttctactcgc ttcccaaact
     1561 ccgtttccaa acgatttctt aacatttggg ctgcaccaaa tcctgttgca caaatagcaa
     1621 gaatattaaa cttagtactc tctttgctac gttccatagc agctaaaaag tgaagactta
     1681 catatgctac ttcatcatct gatattgtcc actccaagaa cttgtccata tttgcaagaa
     1741 tttctctagt cataaagaat atatcactat aattctgttt aatttcatct accaaagggt
     1801 tatttaaggt aattcggctt tctaaacgta cttgtagtgt cattagatga gttatcaatc
     1861 cttcaattag ttggaaatct gaagaaaagt tatacatatc atctaatcct aaattctgaa
     1921 atgttttaag taaagatttt tttaaaactt cttcagaaat attcttctga tttttttgac
     1981 attgttgact cttagctaac aaatgcaaag taatgtagtc tatttcctga actggaaatt
     2041 cctgatttgt tacttctctt actttagaaa gaattctttg ggcaaccttt ctctctattg
     2101 catcatcagt catctgacag tctatatttt ttatttcaaa tccggatttt aaacgaatca
     2161 cagacaatgc tatgtgaact actaaattct gtagtacaaa atcagatagt tttaggttgg
     2221 cctcttggca ttcatccaaa acaattctag caaattcttc taatggaaca gtttgatcaa
     2281 aaaagttaaa ttttacatag caatgtattg ttttaaaaaa ttgattctct aggaaataat
     2341 ttatgataaa acgtcgttta tcacgttcct cgcctgagac ataaactcct ttattcgccc
     2401 tactctcaat ggacaaatta tactctgata acatcactcg tatctttctg aaatcatgag
     2461 ataatgttga acgactaaca taaagttcat cagctaaatc atcaaaaaga actggaactt
     2521 gctcaaataa taatttattt aagataaata ctaaacgatc atcacctttt gaaaccgcag
     2581 ttttcgtata gtcttcttcc agttcataag tttgtctaaa ctcctggtaa gcgccttgat
     2641 tctcaaaaaa tatttgatac ccttgacctt gttttgaaat caaccggact ccttgaataa
     2701 tcattgtctt ctcaattaat ttcagtacat tacggacagt tctatctgaa caggataaat
     2761 attctgccag ttctttgctt gtaacaaaac gttccttatt ttttattaaa aattgaagga
     2821 tatctttctc tttaatgttt aacacattca ttccctccta aaacgtatgt tttcatatat
     2881 tgaagcatat tatacactta aatcagttta tatcaaactc aaaacaattt atcttaacct
     2941 aaatatttat tgacatttca tgtgttcatc aaatattctc aagaatcaaa ttagccattt
     3001 tttcaattcc cattggaata ggaatatagg cttgaggagg tatttgtaca actggttttc
     3061 ctgctttaga accagcctct tcaaattgct taaagtacat ttttgtttga ggactgacaa
     3121 gatacaaatc aaaagctgct gctgcgatag ctttccctcc ttcagtagca ctaatagcat
     3181 caactacaat atctttccct tttcctttta gaaactctgt tgttttctgt gccataagtg
     3241 atgaagacat tcctgctgca caaataatta aagcttttgc cataatattt tctccttttc
     3301 ttaaatccaa tcaaagctgt gctaagttgg cttatttgtt atctattttt attataaaat
     3361 aaagcgtttc caatgacaat tccctcattt tcctaaatga tatggaaaaa aattatttat
     3421 acttcaattt ataaaataaa attattcctg agagtagaaa tgaaacacta tttgctaaaa
     3481 tcaaaggcaa gtctcctata cgaataccat gagcaagcca caatgcaata ccaataactt
     3541 gcataacata catacctaga gcaatagatc ctgtgtcctt tgtcttaact acacgaaaaa
     3601 cttgtggtaa aaatgcaaat gttgttaaaa ttgctgcaat acttccaatc atatgtcacc
     3661 tcaatatgct aaacaaactg agaataatct cagtttgttt atactattct actgattcac
     3721 cgttagatga aataacttcc ttataccagc caaaagattt tttcggggaa cgattataac
     3781 ttcccttccc attgtcatct ttatctacat aaataaagcc ataacgtttc cgcatttcac
     3841 cggtaccagc tgaaaccaaa tcaatacatc cccatggagt ataacccatt aaatcaacac
     3901 catcttcaac tacagccttt ttcatttcac gaatatgggc acctagatat tcaattctat
     3961 aatcatcatg taccatacca tctgctgcaa cttgatctat agctccaaaa ccattttcaa
     4021 caataaagag tggtaagtga tagtggtctg taaaccaatt taacgcataa cgtaaacctt
     4081 ctggatcaat ttgccactcc cattcagaag ccttaacata attatttttc actaaatctt
     4141 ctgtttcaag ataatcaaaa taaggattat tttcacgatg agagtcgata gcaaaggaca
     4201 tatagtaact gaaaccaatg taatctacag tcccaccaag taaatcttct ttatcctggg
     4261 cagtaaaatc aactgaaata ccttttcgtt cccaatactt gaaaatatgc tcaggatatt
     4321 tacctaaaac atgcacatca gcaaaataat aacgcttttg catagctttc attgccatta
     4381 agatatcctt aggattgcaa gtagctggat aaattggaca catcgcaatc atacaaccta
     4441 tttgaaaatc tggattaatc tcatgaccaa tttttacagc tcgtgcagaa gcaactaatt
     4501 cgtaatgtgc tgcttgatac ataattgcct ctctattatc accttcctca tatacaatac
     4561 ctgagttagt aaatggtgca aaatcttcct gataattcgc ttgattattg atttcattga
     4621 aagtcatcca atatttaacc ttatctttgt aacgtttaaa tacgacttct gcaaaacgag
     4681 caaagaaatc aatcaatttc ctatttttcc aaccaccata ttcggtcact aagtgataag
     4741 gcatttcaaa atgagataga gtgatgacag gttcaatacc attctttaag cattcatcaa
     4801 aaagattatc ataaaactgt aatccttctt cattcggctc taactcatca ccttttggaa
     4861 agatacgtgt ccatgcaaga gaggtacgga agcacttgaa tcccatttca gcaaaaagtg
     4921 ctatatcttc tttataacga tgataaaaat ctatcgcctc atgatttgga taatatttac
     4981 cctctaaaac tcccaaagta atttcacgag ctactccatg acgaccagca gtcataacat
     5041 cagcaacact aattcccttg ccaccttctt gccatccacc ttcaagttga tgagcagcaa
     5101 cagcaccacc ccataaaaat ctatctttaa aagtagtcat cttttttcct cctgactttg
     5161 atactcttat tataaacctc aaaccaaaaa atgaaaacgt attctttttc cttattgtta
     5221 aggaaagaag taatttttaa tggaaataga acaatatctt cttgtattct cgtaatgata
     5281 tctttacgat tttcaatact ttcaaactac aaaaactctc acaataattc taattccctg
     5341 tgtctataaa cgacttatcg ctttctggca tcccagaatc atcttctata taacgttcaa
     5401 cttgcatctg caagtgatat ttttttctta aatctaagat tttctgcatt gtctttgatt
     5461 gataatgttt atctaaagtt tcttgattta tccactgatc aataaggaga atagttccct
     5521 ctttttcaat tggtaaaaaa tattcgtatt tcaagttacc tttttgattt ctaatttctt
     5581 caacaaggcc actatcaagc atttctcttg caaactttat tgcactatct ccatcacctt
     5641 tataatatac atgaatagtc aatgtcatct tatatcctcc aaaatcatcc ttcaatttta
     5701 aaaaaacaag tttagatgag gatctaaact tgttttttat gaactaatta tctaacgttt
     5761 tgccattact ttcaat
//
LOCUS       DAVMEF010000122         5669 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMEF010000122 DAVMEF010000000
VERSION     DAVMEF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.122"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            378..998
                     /locus_tag="VYQ30_001616"
     CDS             378..998
                     /locus_tag="VYQ30_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966694.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1084..1527
                     /gene="tsaE"
                     /locus_tag="VYQ30_001617"
     CDS             1084..1527
                     /gene="tsaE"
                     /locus_tag="VYQ30_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1966695.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLSEELQYGV"
     gene            1517..2035
                     /locus_tag="VYQ30_001618"
     CDS             1517..2035
                     /locus_tag="VYQ30_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966696.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKITGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2043..3059
                     /locus_tag="VYQ30_001619"
     CDS             2043..3059
                     /locus_tag="VYQ30_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1966697.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3144..4400
                     /locus_tag="VYQ30_001620"
     CDS             3144..4400
                     /locus_tag="VYQ30_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1966698.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVTNFSEVSSIFKSGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4455..5621
                     /gene="recA"
                     /locus_tag="VYQ30_001621"
     CDS             4455..5621
                     /gene="recA"
                     /locus_tag="VYQ30_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1966699.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         1757 a          931 c         1337 g         1644 t
ORIGIN      
        1 cttgacatta tagtttttct gaaacgttaa ataacctgta aggctgatag ttagattaat
       61 actatcagcc ttataattat gtgactctga gtccgtctcg ctccgtgagg tgcgagacaa
      121 aacaaccacc cgtttgacgg attttcatga tttcaaattt atattcttcg aaaatctctt
      181 caaatcacgt cagctctatc tgcaatctca aaacagtgtt ttgagcaacc tgtggctagc
      241 tacctagttt gatctttgat tttcattgag tattagtctc tagtgtttgt ttagcgtctt
      301 aaagagacat ttgagaagat tagaaacagt tctctctttt ctcctagtat aatgattggt
      361 aggaagggag agagaagatg aaatcaatga gaatcttatt tttgttagct ttaattcaaa
      421 tcagtttgag tagctgtttc ttatggaagg aatgcatctt gtcctttaaa caaagtacag
      481 cttttttcat cggaagcatg gttttcgttt caggaatctg tgctggagta aattatcttt
      541 atacccgtaa gcaagaagtc catagtgtcc tagccagtaa gaagtcggtg aagctttttt
      601 acagtatgtt actcttaatt aatttgttag gagctgttct tgttttgtca gataacttgt
      661 tcatcaaaaa tacgctgcag caagaattag ttgacttttt attgccatcc ttctttttcc
      721 tatttgggct agatttgctg atttttttac ccttgaaaaa atacgtgcgc gattttcttg
      781 ctatgctaga cagaaaaaag acagtgttgg tgactatttt agcaacactt cttttcttaa
      841 gaaatccaat gaccattgtc tcacttctga tttatattgg actgggcttg ttttttgcag
      901 cctatcttgt cccaaattcg gttaagaagg aagtttcctt ttatggtcat attttccgag
      961 atcttgtatt ggtcattgtt acgctcattt tcttttagag aaagcctgtt ttttatggat
     1021 gttatgatga taagtttagt aggaaaatag acacaaaaga aaagtttttg gtataataag
     1081 agtatgtaca taaaaaatga agaagagttg caagccttag gggagcgttt gggccatcta
     1141 ttagcaaaga atgatgtttt aatcttaatt ggagaactgg gtgcaggtaa aacgaccttt
     1201 actaaaggac ttgcaaaagg attacagatt tctcaaatga ttaaaagtcc tacctatact
     1261 atcgtgagag agtatgaagg tcgacttcca ctttatcacc tagatgttta tcgtattgaa
     1321 ggagatgctg attctatcga cttggatgag tttatctttg gtggcggcgt gactgttatt
     1381 gagtggggaa atctcttagg agatgccttg ccagatgctt atttagaatt ggaaattcta
     1441 aaagaagcag atggacgccg tttaaatttt caggcaaagg gtttgcgtgc tgagaaattg
     1501 tcagaggagc ttcaatatgg agtatgaatt gctcattagg gaagcagagc ccaaagatgc
     1561 agctgaatta gtggcctttt taaatcgtgt gagtttggag acagacttta ccagcctaga
     1621 cggagatggt attctcttga ctagtgagga gatggaaata ttcctcaaca agcaagctag
     1681 ttcggacaat cagataacct tacttgcatt cttaaatggc aaaattactg gtattgtaaa
     1741 tattacagct gatcagcgta agagagtccg tcatattgga gatctcttca ttgtgattgg
     1801 aaaaagatat tggaataatg gcttgggaag tttgttgcta gaagaagcga tagagtgggc
     1861 acaagcaagt ggcattctgc gtcgtctcca actgactgtc caaactcgta atcaagcagc
     1921 agtccatctt tatcaaaagc atggctttgt cattgaaggt agccaagagc gtggggcata
     1981 tatagaagaa gggaaattta tcgatgttta cttgatgggt aaactgatag gttagtagaa
     2041 atatggttaa aaaaattatt ggaatggtgc tagctttact ttctgtaact gtagtaggag
     2101 taggtgtttt tgcttatact atttatcaac aagggacaga aaccttagct aaaacctata
     2161 aaaaaatcgg cgaagaaacc aaggttattg aagcgactga acctctaacc attctgttaa
     2221 tgggagtgga caccggaaat gttgaacgaa ctgaaacttg ggtcggtaga agtgatagca
     2281 tgatcttgat gacagtgaat cctaaaacga aaaaaacaac aatgatgagt ttagagcggg
     2341 atattctgac gcgcattgaa tcagggaatg gtcaggctca tgaagcgaaa ctgaactcag
     2401 catatgcaga tggtggagca gagcttgcta tagaaaccat tcaaaaaatg atgaatatcc
     2461 atattgatcg ctatgtgatg gtcaatatga gagggttgca aaaattagtg gatgcagtag
     2521 gaggtattac agtcaataat atcctaggtt tcccaatttc tatcagtgac caagaagaat
     2581 ttaataccat ttctatcggt gttggggagc aacatattgg gggagaagaa gccctagtct
     2641 atgcacgaat gcgttaccaa gatcctgagg gggattatgg tcgtcaaaaa cgtcaacgtg
     2701 aagttattca aaaagtcatg gaaaaagctc tcagtttaaa tagcattggt cattatcaag
     2761 agattctaaa agctttgagt gacaatatgc agaccaatat tgatttgtct gcaaaaagta
     2821 tccctaactt gctaggctat aaagattcat ttaaaaccat tgaaactcag cagttgcagg
     2881 gtgaaggaga gatacttcaa ggtgtttctt accagattgt ttcgagagca catatgttgg
     2941 aaatgcaaaa tatactccga cgttctttgg gacaagaaga agttactcag cttgaaacca
     3001 atgcggtttt atttgaagat ttatttggca gagcacctgt tggtgatgaa gataattaac
     3061 ttttgataca aataaaaaaa tcaatcgtag gaaaattcct gcgatttttt caaaaaaata
     3121 cgaatagata ggtaggagga aacatgaaag cagaaatcat tgctgttgga acagagattt
     3181 tgacaggaca gattgtcaac accaatgccc agtttttgtc ggaaaaacta gctgagattg
     3241 gggtagatgt atattttcag acggctgtag gagacaatga agttcgtctc ttgtctttgc
     3301 ttgagattgc cagtcaacgt agcagtctgg tgattttgac aggcggtttg gggccaactg
     3361 aggacgacct aaccaaacaa accctagcta aatttttagg gaaagcatta gtctttgatc
     3421 ctcaggctca ggagaagttg gatatctttt ttgccctgcg accagactat gcccgaacac
     3481 cgaataacga aagacaagct caaattgtag aaggagcgat tccactgcca aacgaaacag
     3541 gactggctgt tggaggaaaa ttagaagtag acggagtgac ctatgtcgtc cttccaggtc
     3601 cgccaagtga attgaaaccc atggtcttaa accaacttct acccaagttg atgacaggga
     3661 gcaagctgta ttcccgagtt cttcgtttct ttgggattgg cgagagccag ctggttacga
     3721 ttttggctga tttaattgat aatcagatcg atcctacctt ggccccttat gccaagacag
     3781 gagaagtcac tctacgtctg tcaacaaagg ctagcagtca agaagaggcg aatcaagcgc
     3841 tggatatctt ggaaaatcaa atcttggact gccagacttt cgaaggaatt tctttacgag
     3901 acttttgcta tggttatggg gaagaaacta gtttagccag cattgtggta gaagaactga
     3961 aaaggcaagg gaaaaccatc gcggctgcag agagtttgac ggcaggtctt ttccaagcta
     4021 ccgtgacgaa tttttctgaa gtttcaagta tatttaagag tggttttgtg acctatagct
     4081 tggaggaaaa atcaaggatg ttggatattc ctgccaagaa tttggaagaa catggtgtgg
     4141 tgtctgaatt tacagctcag aagatggctg agcaggcacg aagcaagacc cagtctgatt
     4201 ttggaattag tttgactgga gtggcaggac cagatagcct agaaggacac ccagttggga
     4261 cagtcttcat aggcttggcg caagagcagg gaactgaggt tatcaaggtt aatattggag
     4321 gcagaagccg agcagatgta cgtcacattg cggttatgca tgcctttaac ctagttcgca
     4381 aggctttatt aagtgactaa cttttgatat aatagtagat aggtctgagg atcattagaa
     4441 tgtaggagaa tagaatggcg aaaaaaccaa aaaaattaga agaaatttca aaaaaatttg
     4501 gggcagaacg tgaaaaggcc ttgaatgacg ctcttaaatt gattgagaaa gactttggta
     4561 aaggatcaat catgcgtttg ggtgaacgtg cggagcaaaa ggtgcaagtg atgagctcag
     4621 gttctttagc tcttgacatt gcccttggct caggtggtta tcctaaggga cgtatcatcg
     4681 aaatctatgg cccagagtca tctggtaaga caacggttgc ccttcatgca gttgcacaag
     4741 cgcaaaaaga aggtgggatt gctgccttta tcgatgcgga acatgccctt gatccagctt
     4801 atgctgcggc ccttggtgtc aatattgacg aattgctctt gtctcaacca gactcaggag
     4861 agcaaggtct tgagattgcg ggaaaattga ttgactcagg tgcagttgat cttgtcgtag
     4921 tcgactcagt tgctgccctt gttcctcgtg cggaaattga tggagatatc ggagatagcc
     4981 atgttggttt gcaggctcgt atgatgagcc aggccatgcg taaacttggc gcctctatca
     5041 ataaaaccaa aacaattgcc atttttatca accaattgcg tgaaaaagtt ggagtgatgt
     5101 ttggaaatcc agaaacaaca ccgggcggac gtgctttgaa attctatgct tcagtccgct
     5161 tggatgttcg tggtaataca caaattaagg gaactggtga tcaaaaagaa accaatgtcg
     5221 gtaaagaaac taagattaag gttgtaaaaa ataaggtagc tccaccgttt aaggaagccg
     5281 tagttgaaat tatgtacgga gaaggaattt ctaagactgg tgagcttttg aagattgcaa
     5341 gcgatttgga tattatcaaa aaagcagggg cttggtattc ttacaaagat gaaaaaattg
     5401 ggcaaggttc tgagaatgct aagaaatact tggcagagca cccagaaatc tttgatgaaa
     5461 ttgataagca agtccgttct aaatttggct tgattgatgg agaagaagtt tcagaacaag
     5521 atactgaaaa caaaaaagat gagccaaaga aagaagaagc agtgaatgaa gaagttacgc
     5581 ttgacttagg cgatgaactt gaaatcgaaa ttgaagaata agctgttaaa gcagtggaga
     5641 aatccgctac tttttcgatt tttgattca
//
LOCUS       DAVMEF010000123         5501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMEF010000123 DAVMEF010000000
VERSION     DAVMEF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.123"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            344..1387
                     /locus_tag="VYQ30_001622"
     CDS             344..1387
                     /locus_tag="VYQ30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1966700.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1555..3642
                     /gene="topA"
                     /locus_tag="VYQ30_001623"
     CDS             1555..3642
                     /gene="topA"
                     /locus_tag="VYQ30_001623"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1966701.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3748..4107
                     /locus_tag="VYQ30_001624"
     CDS             3748..4107
                     /locus_tag="VYQ30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1966702.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4200..4832
                     /locus_tag="VYQ30_001625"
     CDS             4200..4832
                     /locus_tag="VYQ30_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1966703.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4876..>5501
                     /locus_tag="VYQ30_001626"
     CDS             4876..>5501
                     /locus_tag="VYQ30_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1966704.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELI"
BASE COUNT         1695 a          947 c         1205 g         1654 t
ORIGIN      
        1 agtagagatt acaaggcttg tttaagaaag aattcaaaga ccttgacaaa taaaaataaa
       61 atggttatta taaaaaatgg tctgaaatag atgatgatac ttttcgaaaa tctttttacg
      121 tcagctcagc tttgccttgc tgtgttttga gcaagctacg gttagcttcc gagtttgatt
      181 ttcatttact agaaatgaaa ctgatgagag atatcagtag acatttgagt caggatatta
      241 tggaaaatga taaaaagagc tcgtgagatt ggcatatcag actactaaag tattgagttt
      301 gttaggattt tagcgactag ttagctggga aaggaagata tttgtgacaa ataataaact
      361 gtattcgttg atagaattta gaaataaaat atatgaagaa ttagaacttt ccagaagtga
      421 tttagcgatt ttactatgtg ccatgcttat cgcctctatc ggattaaata tggattcgac
      481 tcccgtgatt attggagcca tgttaatctc tcctttgatg acacctattc tgggagtggg
      541 gctctctcta gctatatttg attttaaatt gttaagaaaa tcttttaaaa tattagctat
      601 tcaaattctt gccagtttaa tagcttcaac actttatttt tatctttctc ccatttcgta
      661 tgctagttcg gagattgttg ctagaacctc tccgactatt tgggatgttc tcattgcttt
      721 tgtaggaggg atagcaggta ttattggtgc taggaaaaaa gagaccaata atattgttcc
      781 tggtgttgct attgcaaccg ccttgatgcc tcctctttgt acagtaggtt atgctattgc
      841 ttctgctaat ctaaaattta tcataggctc ctcttaccta ttcctcatca attgtagctt
      901 tattgtcatt gcgacttata taggtgttag gttgatgatg gttaagaaac attattttaa
      961 agataatgaa gaagactcta aaatgcgtag gattttgctt ctagttgctg ttttgctgat
     1021 gattccgagt ttcatctctg caacgacttt agtgagagaa acgttgaaaa aagagtccct
     1081 taagaaattt atatcagagc agtttcaggg gtataatatt ttgaaaaaaa cctattctaa
     1141 aaagactcat accctaaagc taaccatttc aggaaattat ttgacagaag aagaactcga
     1201 tatgatttca agtaagagag gtgactatgg tttaagtgat gtttctgttc aagtttcaca
     1261 attgtctgat tcagaacaac ttagtaagga agagctggtg gagtatttct tccagtatat
     1321 caaggataag gaagcaaaag aaaaggagaa agccaataaa tttgatacag agtctgagga
     1381 gcaataattt cttgagaata gctggtttct ctcgtgagtc ttctatgtat atcaaaggaa
     1441 gactgaggtt ttaagtataa aacttttctt ctgtttatag tagctaagtc ttgacagggt
     1501 tcaaaaaatg gtttacactt tataaagttt attactttga aaaggtgtga tactgtggct
     1561 acggcaacaa aaaagaaaaa atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct
     1621 gctaaggcca agacgattga aaaatatcta ggcagaaact acaaggtatt agccagtgtc
     1681 gggcatatcc gtgatttgaa gaaatccagt atgtccgtcg atattgaaaa taattatgaa
     1741 ccgcaatata ttaatatccg aggaaaaggc cctcttatca atgacttgaa aaaagaagct
     1801 aaaaaagcta ataaagtttt tctcgcgagt gacccggacc gtgaaggaga agcgatttct
     1861 tggcatttgg cccatattct caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat
     1921 gaaatcacca aggatgcagt caaaaatgct tttaaagaac ctcgtaagat cgatatggac
     1981 ttggtcgatg cccaacaagc tcgtcggatc ttggatcgct tggtagggta ttcgatttcg
     2041 cctattttgt ggaagaaggt caagaagggc ttgtcagcag gtcgcgttca gtccattgcc
     2101 cttaaactca tcattgaccg tgaaaatgaa atcaatgcct tccagccaga agaatactgg
     2161 acagttgatg ctgtctttaa aaagggaacc aaacaatttc atgcttcctt ctatggagta
     2221 gatggtaaaa agatgaaact gaccagcaat aacgaagtca aggaagtctt gtctcgtctg
     2281 acgagtaaag acttttcagt agatcaggtg gataagaaag agcgtaagcg caatgctcct
     2341 ttaccctata ccacttcatc tatgcagatg gatgctgcca ataaaatcaa tttccgtact
     2401 cgaaaaacca tgatggttgc ccaacagctc tatgaaggaa ttaatatcgg ttctggtgtt
     2461 caaggtttga ttacctatat gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat
     2521 gaggcggcaa gcttcattac ggatcgtttt ggtagcaagt attctaagca cggtagcaag
     2581 gtcaaaaacg catcaggtgc tcaggatgcc catgaggcta ttcgtccgtc gagtgtcttt
     2641 aatacaccag aaagcatcgc taagtatctg gacaaggatc agctcaagct atataccctt
     2701 atctggaatc gttttgtggc tagccagatg acagcggccg tttttgatac catggctgtt
     2761 aaattgtctc aaaatggagt tcaatttgct gccaatggta gtcaggttaa gtttgatggt
     2821 tatcttgcca tttataatga ttctgacaag aataaaatgc taccagatat ggctgttgga
     2881 gatgtggtca aacaggttaa tagcaaacca gagcaacatt tcacccaacc gcctgcccgt
     2941 tattctgaag caacactgat taaaacctta gaggaaaatg gggttggacg tccatcaacc
     3001 tatgcgccaa ccattgaaac cattcagaaa cgttattatg ttcgcctggc agccaaacgt
     3061 tttgaaccga cagagttggg agaaattgtc aataagctca tcgttgaata tttcccagat
     3121 atcgtaaacg tgaccttcac agctgaaatg gaaggtaaac tggatgatgt cgaagttgga
     3181 aaagagcagt ggcgacgggt cattgatgcc ttttacaaac cattctctaa agaagttgcc
     3241 aaggctgaag aagaaatgga aaaaatccag attaaggatg aaccagctgg atttgactgt
     3301 gaagtgtgtg gcagtccaat ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt
     3361 agcaatttcc cagattgccg tcatacccaa gcaatcgtga aagagattgg tgttgagtgt
     3421 ccaagctgtc atcagggaca aattattgag cgaaaaacca agcgtaatcg cctcttctat
     3481 ggttgcaatc gctatccaga atgtgaattt acctcttggg acaagcctgt tggtcgtgac
     3541 tgtccaaaat gtggcaactt cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt
     3601 gtttgtagca aaggcgacta cgaggaagaa aagattaaat aagaggaggg gcccgaaagt
     3661 aaatttcagg ctccttttgg ttagaacttg acaaaataca tcattttatg cgaaactaga
     3721 agaagattat tttaactagg agaagttatg cgtcttatct atctgataat tggtttttta
     3781 tcactggcct tggctattgt tggggttgtt ttacccttgt tgccgacaac gcctttcctt
     3841 ttgttgtcta ttgcttgttt ctccagaagt tccaagcgtt tcgaagactg gctttatcat
     3901 actaagctct atcaaacata tgtagctgat tttcgcgaga ctaagtctat tacgcgtgaa
     3961 cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg gaatttctat ttattttgca
     4021 cctcttttac cagtcaaaat cggtctgggt gctttgacca tctttatcac ttattatctt
     4081 ttcaaggtca ttccagacaa agaatagtta aaacagtagt tatttgcctt gataaaattg
     4141 aaagcatatt cataataata tgatataata aatttgaagt aatattcaag gagaatcaaa
     4201 tgatttacga attttgtgct gaaaatgtga ctttacttga aaaagcgatg caggctggag
     4261 ctcgtcggat tgaactctgt gataatctag cagttggtgg gacaacaccc agctatggag
     4321 tgactaaggc agcggttgaa ctggcagcta actacgatac aaccatcatg accatgattc
     4381 ggccacgtgg tggtgacttt gtctataatg acctagaaat tgctatcatg ctagaagaca
     4441 ttcgtttgac tgctcaggct ggaagtcaag gggttgtatt tggaacttta actgctgata
     4501 aaaagttgga taagcctaat ctggaaaagt taattgctgc atcaaaagga atggaaattg
     4561 tctttcacat ggcctttgat gaactaagtg atgaagatca accggaagct attgactggc
     4621 tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg tgtgtctggc gactccttag
     4681 aaaaacgttt tgttcactat cacagaattt tggagtacgc taaaggtaaa attgaaattc
     4741 taccaggtgg ggggattgac cttgaaaacc gtcaaacctt tatcgaccag gtgggggtaa
     4801 cacaattgca tggtactaag gttgtttttt aaaaaataga aaggaactgc tagctttggg
     4861 tagcagtttt cacttatgtt tgaaattttt aaatcctatc aatttaatca agaaaaggct
     4921 catgattatg gttttataga aaatagcgaa gtctggacat atagttgcca gattttgcaa
     4981 ggtgactttg tcatgactgt gtccatcact gctgataatg tgaactttca agtctttgac
     5041 caagagactg gtgacctcta tcctcacgtt catatggaaa gcatgagggg aagttttgtc
     5101 ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga ttcggaaggc ttgttttgat
     5161 gtgcaagatt ttatctgtca tcagactaag cgtatcatga ctcaagttca ggaaaagtat
     5221 ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata cagctgtatt gcgccatgaa
     5281 ggcaatcaaa agtggtatgc cgtcttgatg aaaatctctt ggaataagct ggaaaagggc
     5341 agagaaggac aagtggaagc agtcaacctc aagcatgacc aagtagctaa tttgctttca
     5401 caaaagggga tttatccagc cttccatatg agcaagcgct actggattag tgtgtccctt
     5461 gatgatactt tatcagatga agaagtactg gaattgatag a
//
LOCUS       DAVMEF010000124         5354 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMEF010000124 DAVMEF010000000
VERSION     DAVMEF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.124"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..2253)
                     /locus_tag="VYQ30_001627"
     CDS             complement(<1..2253)
                     /locus_tag="VYQ30_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1966705.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNLLKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDCIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKTEVKSSEKE"
     gene            complement(2482..2712)
                     /locus_tag="VYQ30_001628"
     CDS             complement(2482..2712)
                     /locus_tag="VYQ30_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1966706.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            2903..3586
                     /locus_tag="VYQ30_001629"
     CDS             2903..3586
                     /locus_tag="VYQ30_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966707.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3586..4320
                     /locus_tag="VYQ30_001630"
     CDS             3586..4320
                     /locus_tag="VYQ30_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1966708.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRSQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4474..5331
                     /locus_tag="VYQ30_001631"
     CDS             4474..5331
                     /locus_tag="VYQ30_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1966709.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETTLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
BASE COUNT         1479 a         1029 c         1201 g         1645 t
ORIGIN      
        1 ttctttttca gaacttttaa cttcagtttt tttggcagat ttaatctgaa tgtttccctt
       61 gctagtcata actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat
      121 tgtgtcctca atacagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc
      181 ataacctagg tcaaccaact tttccttgac cttatcagtt acatccaaac gaatgttgtt
      241 gctagagagg cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc
      301 cttgctgaga gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa
      361 gaagttaccg agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc
      421 aacgctagct tcggtctttc ctgtacctgc atttgaggtc atgataatga tagcatcctt
      481 gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa
      541 catgtgcata acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg
      601 attgtggcga actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg
      661 agcgccgacc aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat
      721 catactatca gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc
      781 gacaccagtt ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc
      841 gacacgatta cggcgaatag ccttggcaat cttatcgact gcatcatctt gaccaataac
      901 atgagacttg agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc
      961 accaacaggg atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat
     1021 aggagtatcc tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc
     1081 gcggaagtag gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc
     1141 agcctcaatc aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat
     1201 cttagaacca gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat
     1261 gtagcgattg gaaagagttg cagctgcttc aatcgcagca tcggtatatt gaacgtggtg
     1321 gtaatcttcg tatttctttt gaatcccttt gagaatagtg attgtttcgt ctaccgttgg
     1381 ttcatcgact ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg
     1441 gtattcattg agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt
     1501 gaggagattt cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg
     1561 gatttcatcg ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg
     1621 catgcgttct tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag
     1681 acggatgact tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc
     1741 tagaccttcg acaacggccg tttttccgac accaggttca ccgataagga caggattatt
     1801 cttggttcta cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac
     1861 gggatcaatg tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag
     1921 gcctttttct tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc
     1981 gccgtttcca ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga
     2041 aggtctgaaa ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt
     2101 cagatccgtc atacctttga agaggctatt gttaggatct gttttgataa tcttatagca
     2161 gttttgacag aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt
     2221 tgagtcgtta attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc
     2281 tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg
     2341 tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa
     2401 tttggactat aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg
     2461 acaatcccag cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc
     2521 aatgctatca aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc
     2581 gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt
     2641 acgttttaaa tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa
     2701 atgtgattcc attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg
     2761 ccaacttgac tagaaaaaaa ctaagatttc tcgctaattc tgctagttta aagattttta
     2821 aataaattgg ataaaaaatt taaattttaa ttcaaaacat gttataatca tacagtattc
     2881 tattttagaa agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt
     2941 taactatggg gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat
     3001 tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc
     3061 caacttgtac gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt
     3121 tgctcttatg catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc
     3181 gcgtctgatt ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagac
     3241 tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg
     3301 gattcgtcct aaaaatgcga tgcgttatgt gattttgcca caagcagtca aaaatatctt
     3361 gccagcattg gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat
     3421 tggggtcatg gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt
     3481 aacaccactt ttatttgcag cattttacta cttgattatg acctctattc tgacagtagc
     3541 cttgaaagct tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg
     3601 ataaaaattg aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac
     3661 ctcgagatta aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct
     3721 accttgcttc gctctatgaa tttgttggag gaagcaacca aggggaagct tatctttgag
     3781 ggagtcgata ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg
     3841 gttttccaac aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca
     3901 cctatcaaga ctaaaggtga aagcagggaa gttgctgaga agagatccca agagcttttg
     3961 gaaaaagtgg gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcaa
     4021 caacaacgga ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac
     4081 gaaccaactt cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac
     4141 cttgccaagt cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag
     4201 gtggcagatc gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag
     4261 cagatttttg aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa
     4321 tctattttaa aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag
     4381 tttgaaacta taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat
     4441 aatggaagat atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct
     4501 ttagcggcca aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaagaaaca
     4561 actctagtgc ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac
     4621 gttcgcaaca aggagaggtc agctcttgcg gctggttccc gtagcgaagt agtgcgagtt
     4681 ccagagacca ttactcaaga ggaattgtta gacctgattg ctaaatataa tcaggatcca
     4741 gcttggcatg ggattttggt ccagttgcca ttaccaaaac atatcgatga agaggcggtt
     4801 ttattagcca ttgacccaga aaaggatgtg gatggtttcc atcccctaaa catgggacgt
     4861 ctttggtctg gacatccagt catgattccc tcgacacctg caggaattat ggaaatgttt
     4921 catgaatatg ggattgactt ggaaggtaaa aatgcggtcg taatcggtcg ttcaaatatc
     4981 gttggaaaac ctatggctca gcttcttttg gctaaaaatg cgacagtaac cttggcccac
     5041 tcacgaactc ataatcttgc caaggtggct gctaaagcag atattcttgt agtcgcaatc
     5101 ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag gtgcggttgt cattgacgtt
     5161 gggatgaatc gagatgaaaa tgggaagctc tgtggagatg ttgattatga ggcagttgca
     5221 ccacttgcta gccatatcac gccagtccct ggaggtgtcg gtcctatgac cattaccatg
     5281 ctgatggagc aaacttatca agcagcactt cggacattgg atagaaaata agagaaacat
     5341 tcgattaata gagt
//
LOCUS       DAVMEF010000125         4901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMEF010000125 DAVMEF010000000
VERSION     DAVMEF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.125"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            31..1206
                     /gene="mutY"
                     /locus_tag="VYQ30_001632"
     CDS             31..1206
                     /gene="mutY"
                     /locus_tag="VYQ30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1966710.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1261..1965
                     /locus_tag="VYQ30_001633"
     CDS             1261..1965
                     /locus_tag="VYQ30_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966711.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            1958..3307
                     /gene="vicK"
                     /locus_tag="VYQ30_001634"
     CDS             1958..3307
                     /gene="vicK"
                     /locus_tag="VYQ30_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1966712.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3309..4118
                     /locus_tag="VYQ30_001635"
     CDS             3309..4118
                     /locus_tag="VYQ30_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1966713.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4209..4451
                     /locus_tag="VYQ30_001636"
     CDS             4209..4451
                     /locus_tag="VYQ30_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1966714.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4453..4707
                     /locus_tag="VYQ30_001637"
     CDS             4453..4707
                     /locus_tag="VYQ30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1966715.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4754..>4901
                     /locus_tag="VYQ30_001638"
     CDS             4754..>4901
                     /locus_tag="VYQ30_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1966716.1"
                     /translation="MNKKWLFADYYDITIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIW"
BASE COUNT         1498 a          842 c         1123 g         1438 t
ORIGIN      
        1 aggtgaaacc ttctgtgtta tactagagat atgttagatt tgaaagaata cggtatcgtc
       61 atgtggccgg aggagaaagt catttctttc cgtgagaaac ttctcgcttg gtatgatgaa
      121 aacaaaagag atttgccttg gaggagaagt aaaaatcctt atcacatctg ggtatctgaa
      181 atcatgcttc agcagaccag ggtggataca gttatccctt actacgaaag attcctggac
      241 tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg agagtttact gaaagcttgg
      301 gagggcttgg gctattattc tcgagttcgc aatatgcagg ctgcagccca gcagattatg
      361 actgactttg gtggccaatt tccaaatacc tatgaaggaa tttccagctt gaaagggatt
      421 ggaccttaca cagcaggagc catttccagt attgctttta acttgcctga gccagctgta
      481 gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag tcaaccacga tattgggatt
      541 ccaagtaatc gaaaaatttt tcaggcaatg atggagatct tgattaatcc ggatcgtccg
      601 ggtgatttta atcaagcctt gatggactta gggtctgata ttgagtctcc tgtaaatccc
      661 agacctgaag aaagcccagt caaggacttt agtgcggcat atcagaatgg aaccatggac
      721 cgttatccaa ttaaatctcc taagaaaaag ccggttccta tttatcttaa agccttagtt
      781 gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg aaagcgaaaa gctcttggca
      841 ggtttttggc atttcccctt tatagaagtt gataactttt cgcaagaaga gcagtttgac
      901 ctctttcatc aggttgcgga agaaagtgtg aactttggcc ccagtccaga agagagtttc
      961 cagcaggact atgacctaga tgttgattgg cttgatgttt gttttgatac agtccagcat
     1021 gtttttagtc atcgaaaatg gcatgtacag attgtagcag gtcaggtgag tgacttccat
     1081 gatttttcag atagggaagt tcgttggctt tcaccagaag aattcaagaa ttacccactt
     1141 gccaaacccc aacaaaaaat ctggcaggct tatgcacaag ccaacttaga cagtagccaa
     1201 gactagctat tgtgtcttct gactattttt tggtataata gtagagaaaa aggtgaacat
     1261 atgaaaaaaa tactaattgt agatgatgag aaaccaatct cggatattat caagtttaat
     1321 atgaccaagg aaggttatga agttgtaact gcttttaatg gtcgtgaagc gctagagcaa
     1381 tttgaagcag agcaaccaga tattattatt ctggatttga tgcttccaga aattgatggt
     1441 ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc ccattcttat gctttcagcc
     1501 aaagatagtg aatttgataa ggttatcggt ttggaacttg gggcagatga ctatgtaacg
     1561 aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag ctcttctgcg tcgttctcaa
     1621 cctatgccag tagatggtca ggaagcagat agtaaacctc aacctatcca aattggggat
     1681 ttagaaattg ttccagacgc ctacgtggct aaaaaatatg gcgaagaact agacttaacc
     1741 catcgtgaat ttgagctttt gtatcattta gcatcgcata caggtcaagt catcacgcgc
     1801 gaacacttgc ttgagactgt ctggggttat gactattttg gtgatgtccg cacagttgat
     1861 gtgactgtac gacgtctgcg tgagaagatt gaagatacgc ccagccgacc agagtatatc
     1921 ttgacgcgcc gtggtgtagg gtattacatg agaaataatg cttgatttac tgaaacaaac
     1981 catttttacc agagatttta tctttatcct gattttgtta ggtttcatcc ttgttgtgac
     2041 cctcttatta ctggaaaata gacgtgataa tattcagttg aagcaaatca atcaaaaggt
     2101 taaagatttg attgcaggag attattccaa ggttcttgat atgcaaggtg ggtctgaaat
     2161 caccaatatt accaataatt tgaatgactt gtcggaggtt attcgtctca ctcaggaaaa
     2221 tctagaacaa gagagtaaga ggctaaatag tattctgttt tatatgacag atggggttct
     2281 tgcgactaac cgtcggggtc agattatcat gattaacgat acagccaaga agcaactggg
     2341 gttggttaag gaagatgttc tgaatagaag cattttggaa ttgctcaaga tagaagaaaa
     2401 ctatgaattg cgtgatttga ttacccaaag tccagaattg ttgctagatt cccaagatat
     2461 caatggcgaa tatttgaacc ttcgagttcg ctttgccttg atacgtcgag agtctggctt
     2521 tatttcaggt ttggtggctg ttttgcatga tacgacggag caggagaagg aagaacgcga
     2581 acgaagactc tttgtttcca atgttagcca tgagttacgg actcctctga ctagcgtaaa
     2641 atcctatctt gaagccttgg atgagggggc tttgtgtgaa actgtagcac cagactttat
     2701 caaggtttct cttgatgaga ccaaccgtat gatgcgcatg gtgacggatc tcctccatct
     2761 ttcacgtatt gataatgcta ccagtcacct agatgtggaa ctgattaact tcactgcttt
     2821 tattaccttt atcctcaatc gttttgacaa gatgaaagga caggaaaagg agaaaaaata
     2881 tgagctggtg agagattatc ccatcaattc tatctggatg gaaattgata cagataagat
     2941 gacgcaggtt gtcgacaata ttttaaataa tgctattaag tattcgccag atgggggtaa
     3001 aatcactgtc agaatgaaga caactgaaga ccagatgatt ttatccattt ctgaccacgg
     3061 tttggggatt cctaagcagg atttaccacg tatctttgac cgtttctatc gtgtggatcg
     3121 tgctagaagt cgtgcacaag gtggtacagg tctaggactg tctatcgcta aagaaattat
     3181 caaacaacat aagggcttta tttgggccaa gagtgaatac ggcaagggtt caacctttac
     3241 cattgtactc ccttatgata aggatgcagt gaaagaagaa gtatgggagg atgaagtaga
     3301 agactagaat gagtgaaata ggctttaaat acagtatttt agcgtcgggt tccagtggaa
     3361 attcttttta tctggaaacc tcaaaaaaga agcttttagt agatgcaggc ttgtctggca
     3421 agaaaattac cagtctgcta gctgaaatta accgtaagcc agaagacctg gatgccatct
     3481 tgattaccca tgagcattca gatcatatcc atggagtagg cgttttggct cgcaagtatg
     3541 gtatggatct ttatgccaat gaaaagacct ggcaagctat ggaaaatagt aaatatcttg
     3601 gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg taaaaccaaa acctttggag
     3661 atatcgacat cgagagtttt ggtgtaagcc atgatgcagt cgcaccgcag ttctatcgct
     3721 ttatgaagga tgataagagt tttgtcctct tgacagatac aggttatgtc agtgaccgta
     3781 tggcgggcat tgtcgaaaat gcggatggtt atcttatcga ggccaaccat gatgtagaga
     3841 ttttgcgatc aggttcttac gcttggcgac tcaaacaacg aatcctatct gaccttggtc
     3901 acctttctaa cgaggacggt gctgaagcta tgattcggac gctaggaaat cgtactaaga
     3961 agatttacct tgggcattta tctaaggaaa acaatatcaa ggaactggct catatgacca
     4021 tggtcaatca gctggctcaa gctgatctgg gagtcggagt agactttaag gtttatgata
     4081 cctcaccaga taccgcaaca ccattgacag agatatagaa agaacgctga gaaggtgttc
     4141 tttttatatt gactgaacac ctaaaaagta atacaatggt gttaccatta aaaaagggag
     4201 cacaaaagat gactactatt acattaaaag tttctgaagc tgataaaaca tttatgaaag
     4261 caatggctaa gtttgaagga gtttcccttt cggaacttat tcgcaccaaa actcttgaag
     4321 ccctagaaga tgaatacgat gctcgtgtgg cagatttagc ctatcaagag tatttagaag
     4381 acttggaaaa aggagttgaa cccattactt gggaagaaat gatgcatgat ttaggcttga
     4441 aggatgaata atttgtataa attagttcca acaagacgtt ttatcaagca attgaaaaaa
     4501 ttggaccgtt atacgcagaa gctaattaca aactatttac aaaccaatgt tttggaagac
     4561 ccaagacgac acggaaaggc tttggttggt aatcgcgttg gtcaatggcg ctatagaatt
     4621 ggtaattatc gagttatcgt acaaattgta gatgatgaat tagttgttgc tactctagaa
     4681 gttggtcatc ggagagatat ttattgaatt aaagctgagt aataacagta tagaaagtaa
     4741 ggatagtaaa aatttgaata aaaaatggtt atttgcagat tattatgata taacaattat
     4801 tttacttgct ttaatctctg ttattctggt attacttgga tttgctgaga tgattgattt
     4861 ggacaatccg ccttatagta ttattgattt agtaatctgg g
//
LOCUS       DAVMEF010000126         4872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMEF010000126 DAVMEF010000000
VERSION     DAVMEF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.126"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            69..140
                     /locus_tag="VYQ30_001639"
     tRNA            69..140
                     /locus_tag="VYQ30_001639"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:102..104,aa:Glu,seq:ttc)
     gene            146..219
                     /locus_tag="VYQ30_001640"
     tRNA            146..219
                     /locus_tag="VYQ30_001640"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Asn,seq:gtt)
     gene            complement(260..802)
                     /locus_tag="VYQ30_001641"
     CDS             complement(260..802)
                     /locus_tag="VYQ30_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1966717.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1062..2315)
                     /locus_tag="VYQ30_001642"
     CDS             complement(1062..2315)
                     /locus_tag="VYQ30_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1966718.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2671..4428
                     /locus_tag="VYQ30_001643"
                     /pseudo
     CDS             2671..4428
                     /locus_tag="VYQ30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4458..>4872)
                     /locus_tag="VYQ30_001644"
     CDS             complement(4458..>4872)
                     /locus_tag="VYQ30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1966719.1"
                     /translation="TFNINVSFPGNSQVSFESPTFYRLDTKTFTEAIQKIKEQPVTVS
                     TSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQTGFMKVDIPKGKGTI
                     TLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
BASE COUNT         1497 a          837 c          971 g         1567 t
ORIGIN      
        1 aaaaatctca aaagtgattg acaattagca agaaattgat ataatggtta tatctgctac
       61 agatagaagg tccgttggtc aaggggttaa gacaccgcct tttcacggcg gtaacacggg
      121 ttcgaatccc gtacggacta ttttatccgc catagctcag ttggtagtag cgcatgactg
      181 ttaatcatga tgtcgtaggt tcgagtccta ctggcggagt atagataaaa gggaacacag
      241 ctgtgttcct ctttttgtat caatttgtat caccaagcat tttcataagg aagtctgtta
      301 tttcttgaga actttctttt tttccatgtg caatccaagt ttggcagaca ccaaaaagtg
      361 catgagttag atagatgcta ctatattcta attcagtggt atttagattc agttgcataa
      421 atcgcttttg taaatctgta ctaagcatga tatgaagttt atttcgtaag aaattttgga
      481 tttctttagt cccattttca gaaagaaggg cagccagaag tggttctgac tctagatatt
      541 caaaaacttc taaaatagcg tctcttttgt gatgagcatg tttttgaaaa atatattcaa
      601 atgtatggaa tagcttgctt tgatagtgct caatcatatc atacttatcc ttatagtgag
      661 tatagaagct ggaacgacta attccggctt tttctactaa tttgacagta gaaattttat
      721 caaatggctg ttccatcagt aattgtacca tagcgttttc aatagttcgc tttgttttta
      781 agcgtttgtt actttcttgc atatttcctc cttgtaaaca aattagacta tatgtctaaa
      841 aatagatttt ttatcttgta atttagattt tttaatgtat aatctattat atcaaaattt
      901 tagacaatat gtttaaaaaa gtctagtggt aaaatgtcaa gcgacccccc gctcaaagtt
      961 tgaaaactat ttcaaaaaaa gggtgcacta aagaagccct actgaaaacg atgttttcac
     1021 actgttttta gtagattttc aaggagaggt gcagatataa cttagttatc ttccataatc
     1081 ctccttggtg gcgtatagag ttgatggttg cgtagtagca catccaccag acgcacaaat
     1141 tttctagcgg ttagtaagat ggctcgtttg tgttggtgtt taggtacttc atggtatttc
     1201 ttcttataga aatccttgta ttcgtcatca taacgcctta ccgagttggc ggcttcaacc
     1261 aggtagtagc gcagataacg attgccccta ttcacaagcg aagtatgttg tgagtttgag
     1321 ttaccagatt gcttttctct ccaattcaga ccagcaaatt ttgcgacctg gggatggtct
     1381 ttaaagcgtt cgatttgtcc aatttcggca atgataccag cagcgtagac cttcccgacc
     1441 ccaggtatgg aggtcaggca ttgatattca ggaatggttt ccaccatatc ttcaatggcc
     1501 ttatcaattt ccttgatcat tttttccagg tttcgaattt cccgagccag aagtccgaga
     1561 atgacattga ctgaatcttg ttggagttta gagaggcgat aagaacctct gatagcggct
     1621 tgaatggttt gagcaagctt ctcagagtcc ttaaaatgcc ctttacccag tttttggagc
     1681 caatcatcta attcttgaag aggcatgtta gcaagttcgt ctaaggtcat ctcatctgtc
     1741 atcaaggtca tcatcgttga actccatagg ttagtagaga gttcttcttt cttgaattga
     1801 taagccaagg tattgcactt gtagtagata ttttcaatga agtgctgttt ggttctggtt
     1861 aattgctcga tgatttggag tctggttctt gtgagatgtt ggagagccat gtatttctct
     1921 tcttttaaaa aagaaggtga aaatcgctca atacggaaat aatccgcaat gtagaaagca
     1981 tcaatggtat cgtttttgtt ttcttcgaaa gcatcccgat acttcttgat tttattaggt
     2041 tgttcgacca taatctcaac atgtagttct ttcaactgcc tatcttcatg gaagaacata
     2101 gcagggtgga aactgtagag actggttgct tccatgccaa tgacaatcct ttcgaagtga
     2161 taggtatgat tcaaatcaag gatatgattc ttaatctctg ttgccccaga aaggtcatta
     2221 gagagggaag cagtgacggg ggtgctagta tcactcaaca tgatacacac atctaatttt
     2281 gtagagctga tgtctaaacc gacaaaacat ttcattaagg aaggtctcct ttccattaga
     2341 tttggaagtt tttttgacta ctgtaaggtt tcccagaaac accttgttcc aacatcctcg
     2401 cataagagaa tccttatcac agcctgacag agccgcctgc atcctactag atacagaagt
     2461 caagctgtgg aaacagctag tgtgttggaa gtttgaacaa ggcttgggta acagactttt
     2521 taatgaagtc caagcaacaa ggagaaaaag aaacaaccat gagtccattc catgactcta
     2581 ttgttctaga aaaccttgca atagtcaagt gataacgaat aaaacttggg attgaaatat
     2641 ccccatgatt atatattacg aggagaaact atgtttaaag aatggaaagc aatttaaaaa
     2701 aaacaacctt tattattgtc atgatcggga tttctcttat tccagctctg tacaatatca
     2761 tatttttgtc atcaatgtgg gatccatatg ggcaattgtc tgacttacct gtggcagttg
     2821 taaataatga taaagaggct tcctataatg gtaatactat ggcaatagga aaagacatgg
     2881 tgtccaattt aaaagaaaat aaaaccttgg attttcattt tgtagatgaa gaggaaggaa
     2941 agaagggatt ggaagatggc gattactata tggtagtgac tttaccaagt gatttatctg
     3001 aaaaagcaac tacattatcc aatattcaat cgacagcagc ttatcaatca ttgacaagtg
     3061 agcaacaaac tgagataagt gattctgtat ctcaaaattc aactgatagt attcaatcgg
     3121 ctcagtcaat tgtagcttta gtacaagatt tacagggaag tttagaaaac ttacaaaatc
     3181 aatcttctaa tctttcgact ttaaaaaatc aagctaatca agtattacct attacttcta
     3241 cttctttgat aggattgtca agtggattaa cagagataca aggagctgtt actagcaaat
     3301 tagttcctgc cagtcagtcg attgcatcag gtgtaaacgc atatactaca ggtgttgata
     3361 aagtttctca gggcgcaagt caactaagtg aaaaaaatgc taccttgaca ggtagtttgg
     3421 atcaattagt ttcaggctca aacaccttga cacaaaaatc ttctagcttg acagcaggag
     3481 ttggtcaatt agttgaaaaa actccagaat tagtatctgg tattgaaaaa ttatcaactg
     3541 gctccaatta attgaatcaa aagagtcaag aattgatagc aggagttgat aaattgcagt
     3601 caggctctag ccaactagct gacaaatcca gtcagttact ttcaggtgtt ctcaattaga
     3661 aagtggagct aataaattgg cagatggagc tgggaaacta gcagaaggtg gaacaaagtt
     3721 aacttctgga ttggaaggtt tacagacagg acttgcttct ttaggacaag gactaggtaa
     3781 tgctagtgat caactcaaat cagcatcaac agaatctaaa aatgcagaga ttttgtcaaa
     3841 tccactcaat ctttccaaaa cagacaatga ccaagttcct gtaaatggaa ttgcaatggc
     3901 tccttatatg atatcagttg ctctttttgt tgcagcaata tcaacgaata tgatctttgc
     3961 gaagttacct tcaggacgtc atccagagag ccgttgggct tggttgaaat ctcgagctga
     4021 aataaatggt attatagctg ttttggcagg gattttggta tatggaggag ttcatcttat
     4081 tggtctaact gctaatcatg aaatgagaac atttattctc atcatcttaa caagtttagt
     4141 attcatgtct atggtgaccg ctttaacaac atggaatagc cgtataggag ctttcttctc
     4201 gcttattttt cttttattac agttagcatc aagtgcaggt acctatccgc ttgctttgac
     4261 aaatgatttc tttagagcta ttaatccttg gttgccaatg agttattcag tttcgggatt
     4321 acgacaaaca atctccatca acaagtcatt ttcctagctg tcatactagc tctatttact
     4381 ggtttaggta tgctagccta tcaacctaag aaaatggaag aagattaaaa aaatcgaccg
     4441 attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat
     4501 agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa
     4561 agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt
     4621 ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg
     4681 aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt
     4741 gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct
     4801 aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg
     4861 atattgaaag tt
//
LOCUS       DAVMEF010000127         4868 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMEF010000127 DAVMEF010000000
VERSION     DAVMEF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.127"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(4..2457)
                     /gene="phtA"
                     /locus_tag="VYQ30_001645"
     CDS             complement(4..2457)
                     /gene="phtA"
                     /locus_tag="VYQ30_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1966720.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNEDVDSLLKQLYALPLSKRHVESDGLVFDPAQITSRTARGVAVPHGDHYH
                     FIPYSQMSELEERIARIIPLRYRSNHWVPDSRLEQPSLQPTPEPSPGPQPAPNLKIDS
                     NSSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKK
                     ENVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDD
                     LLAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAY
                     VTPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRV
                     KGEKRIPLVRLPYMVEHAVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDL
                     FATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEV
                     PQVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEK
                     LLALLKGSNPSSVSKEKIN"
     gene            complement(2711..4444)
                     /gene="ptsP"
                     /locus_tag="VYQ30_001646"
     CDS             complement(2711..4444)
                     /gene="ptsP"
                     /locus_tag="VYQ30_001646"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1966721.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4450..4713)
                     /locus_tag="VYQ30_001647"
     CDS             complement(4450..4713)
                     /locus_tag="VYQ30_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1966722.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1264 a         1033 c          929 g         1642 t
ORIGIN      
        1 tcattagttt attttttcct tacttacaga tgaaggatta cttcctttta acaacgcaag
       61 taatttttct gcttctgcca tgatactatt gttatccata atttgaagag tcaaattatt
      121 tcgtaaacca gctagagttt ctgttgcatt ggctttcaga ctagaatccg ttactttcgc
      181 aagcaaaact tctgcttctt tgagttgggc ttctactttt tcagtctcta cttgagggac
      241 ttctggctca gcaggtgttt cctctactgg ctcttcatcc gctttgaagt tcttatttgg
      301 atcttcactg tggtctttct tgcctaacac atgctcactg gcattgcccc atccatcatt
      361 agaatgtgga cgttcgtcag ggtgttctac gtagtactta atcgtcgcaa acaaatcttc
      421 caaggtatag ccatttggag ctttgtatgt gtgatcatca aaccaagcaa atttaatatt
      481 atggtaatga tccttatgag gaataatcaa attaccgttt ttaacctcaa ctgcatgctc
      541 aaccatatat ggaagtcgaa cgagtggaat tcgtttttcc cctttcacac gattgtaaat
      601 agctgctgca ctatctccag ttggatttgc tttaacatct gcgtctggag atggaggtag
      661 gatacctttt tctttagtat aggcttgagc tgcaactttt tccttatcag aaaggctgtc
      721 ttttccaatc cagtgactat ggcccatatg aggcgttaca tatgcatctc cttcatcact
      781 gattatatca tgttcatcaa aaatgtaacc atctgacgtt gtatacttat cagctaattg
      841 agcaatacga acttcgtctt cagtatactc aatttgagaa tttggcttgc caagtcgctc
      901 tggatgggta attggtgcta ggaatgccaa taaatcatct accaattttt ctttattagt
      961 cgattcatca ttcaagcgtt ctaataattt gtctaaggct tggaaatcag aattacgacc
     1021 cttattttca aacaaggctt tatgagcctc agttaacaga ttatatgctt tatcataaaa
     1081 ttcttggtca cgaggagcaa cattttcttt tttagcagtt aaagtgtgtg aaacactctc
     1141 ttgttttgat aacttgcttt caagattttt aacagtttca gatggtaaat ctttcgcaaa
     1201 gacataacga gagatgccct tttcttcgaa tacatatcct tccccaactt ttcgtaccag
     1261 ctgactaacc aaagaagaat ttgagtctat tttaagattt ggtgcaggtt gcgggcctgg
     1321 actaggttcc ggagtcggtt gtagacttgg ttgttctagt cttgaatccg gtacccaatg
     1381 gtttgaacga taacgaaggg gaataatacg agcgattcgt tcttccaatt cagacatttg
     1441 agagtaaggg atgaagtggt aatgatctcc gtgtggcact gcaacacctc tagctgttcg
     1501 acttgtgatt tgtgctggat caaagacaag gccatcagat tctacgtgtc gtttgctgag
     1561 tggcaaggca taaagttgtt tcagaaggct atcaacatcc tcattacttt gacttgcttg
     1621 actgttagtg ttgctgttgt tgcttgtgtt agtatttgta gttcctggat tgcttacaga
     1681 aggtacccag tttgttcttg aagtgttatc gctattttgt cggcgatagg ttcttgaatt
     1741 tgacagattt ccccgaccag ataggaaggc ttctgcagca gctaactcgc tagctgataa
     1801 ctcattctta ggaatgtaat ggtaatggtc gccgtgagga acgatataag catcaccagt
     1861 atcctctatg atatcagaag cattaaagat ataaccatca tctgtagtat agcgtccttg
     1921 cgaacgtgcc aaggcaacag ctccatcgtt tcttggagtc ccaccttcac gatgttgact
     1981 atgttcttgt ttttgtcgat tgatttcctc ttttgtacgg acgttatccg cgtgggcagc
     2041 atccttaagg taaacatagt attttccatc taccttgata acatatccac ccttgacctc
     2101 attaacaata tcctcatctt ttagcttata gtttggatct ttcatgagta attcttcact
     2161 gaaaatcgca tcataaggaa ccttaccatt gtaataatga tagtggtcgc catgtgaagt
     2221 gacatagcct tggtctgtta tcttgatgac gatttgctca gcattgattc cttcacgctt
     2281 gctaacctca tcaggagtca aattctccgt tttttgcgtc gcttgttttc catctatata
     2341 ggaaacacga ttattttcct taaccgttct agcttgatac agtcccaact cgtaagaaca
     2401 aacacttaaa atcaaagctg ccgcagaacc aacaaggtat ttcttattaa ttttcatcga
     2461 aactctcttt ctatttgata tttctagttg caattcattt ctaaattgca gaaactcctc
     2521 tctattactt aactggttaa tagtttactc ctaaatctcc aacttgtcaa gaattttatg
     2581 aaccatttaa gtattttttg ttctgaccac tactttttca aggttggact ttctataaaa
     2641 aagattttct tgaaaattct ttttaaaata tcaaaaaaat ccctgtaact gagttgcagg
     2701 gatttttcga ttaatcaaaa ttaacgtatt ctttttgaag ttcaagaact tcttccattg
     2761 ttgagcattc tgtaagggca cggtttgcgt actcttccat cttagctgtg tcgagtttct
     2821 tcatcaagct gcgtgtacga agtacagatg ttgctgacat agagaactca tccaagccca
     2881 ttccgacaag aagtggaaca gcttgttggt caccagccat ctcaccacac ataccagccc
     2941 atttaccttc agcgtgagct gctttgatca cattgttaat caagcgtagg attgatgggt
     3001 tgtatggttg gtaaaggtat gaaacttgtt cgttcatacg gtctgctgcc attgtatatt
     3061 ggatcaagtc gtttgtacca attgagaaga agtcaacttc tttagcaaat tggtctgcaa
     3121 gcatagccgc tgcaggaatc tcgatcatga taccaacttg gatgttatcc gcaactgcaa
     3181 caccttcagc aagaaggttt gctttttctt catcaaagac tgctttcgct gcacggaatt
     3241 ctttcaagag cgcaaccatt gggaacatga tacgcaattg accgtgaaca gacgcacgaa
     3301 gaagagcacg gatttgtgtg cggaacatag catctccagt ctcagagata gagatacgaa
     3361 gagcacggaa tccaaggaat gggttcattt cgtgaggcat atcgaagtaa ggaagttcct
     3421 tatctccacc gatatccatt gtacgaacga caacaggttt accgttcatt ccttcaagaa
     3481 cagccttgta tgcttcatac tgctcatctt cagttgggaa gtcttgagaa tccatgtaca
     3541 agaactctgt acggtaaagt ccaacagctt ctgcaccgtt gttgttaaca ccttcaacgt
     3601 cttttggagt accaatattg gcagccaact cgaagtgttt accgtcagca gtcactgttt
     3661 gagcatcttt caaaagtgcc cattcagctt tttgtttcgc ataggcttca ccagctgctt
     3721 taaattctgc cgcttgttca tctgttgggt tgataatcac ttctccagtg atcccgttaa
     3781 cagcaaggat gtcaccgtct ttaacgattt cagtgatgtt atttgtacct aatacagcag
     3841 caatttcaag tgtacgtgcc atgatagctg agtggcttgt acgtccacca atgttggtta
     3901 caaaagcttt tacaaagttt ttgtccaatt gagctgtatc agatggtgtc aagtcatgcg
     3961 caatcacaat cacttcttca ttgatagaag ctgggtttgg caatttttta ccaagaaggt
     4021 ttgccaatac acgttttgtc acgtcgcgga tatccgctgc gcgttcttgc atgtatgggt
     4081 tgtcttccat gccttcaaag atagtgataa acatatctgt aacttctttc agacctgctt
     4141 ctgcattcac tttcttcgca cggatagttt ccttgatttg gctgatcatt tctgggtcag
     4201 caagaaccat taagtgagca tcaaaaactt gagctgcttc ttcaccgagc gtacctactg
     4261 ccttctcgcg aataacagaa agctcgtctt gtgatgcctg tagagcggca tcaaggcgag
     4321 cttcttctgc gtttgtatct tcgactgtaa tagtctcaaa tgacaaatcc ggctgaacga
     4381 gtagatatgc ttttgcaact gcaacaccgt cagatgctgc gattccttta agcatttctg
     4441 tcatttccct tatgccaatc cttctttttc cattgtttct gagattgcag cgatagcgtc
     4501 atctgcatct gcaccttcag ctgagatagt tacgtcagca ccttggccaa caccaagact
     4561 cataacaccc ataattgatt taaggttaac tgatttacct ttgtactcaa gagtgatatc
     4621 tgaagcaaat ttgctagcag tttgtaccaa caatgttgct ggacgtgcgt gaatacctgt
     4681 ttctgccact acgtggaaat ctttagaagc catagtttga ctctcctttt gttctttctt
     4741 tttttgagtt atatgtgata acccttacaa tttagtatta taccatcttc taggattttt
     4801 ttcaagcatt ttctggattt tccttcaatt tcctttcaga taacttgctg aaaatcttct
     4861 atcctaga
//
LOCUS       DAVMEF010000128         4798 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMEF010000128 DAVMEF010000000
VERSION     DAVMEF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.128"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..1195
                     /locus_tag="VYQ30_001648"
     CDS             17..1195
                     /locus_tag="VYQ30_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1966723.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            1419..2198
                     /gene="rpsB"
                     /locus_tag="VYQ30_001649"
     CDS             1419..2198
                     /gene="rpsB"
                     /locus_tag="VYQ30_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1966724.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            2276..3316
                     /locus_tag="VYQ30_001650"
     CDS             2276..3316
                     /locus_tag="VYQ30_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1966725.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAVVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            3562..3885
                     /gene="tnpB"
                     /locus_tag="VYQ30_001651"
     CDS             3562..3885
                     /gene="tnpB"
                     /locus_tag="VYQ30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1966726.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKDFS
                     ITPQI"
     gene            complement(4026..4315)
                     /locus_tag="VYQ30_001652"
                     /pseudo
     CDS             complement(4026..4315)
                     /locus_tag="VYQ30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4312..4710
                     /locus_tag="VYQ30_001653"
     CDS             4312..4710
                     /locus_tag="VYQ30_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966727.1"
                     /translation="MDAPIPKAPLAHSFGSASIIAHIIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEIANWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT         1596 a          890 c          993 g         1319 t
ORIGIN      
        1 ggagtatcta cgtaatatga agaaaaaaat cttagcgtca cttttattaa gtacagtaat
       61 ggtttctcaa gtagctgttt taacaactgc gcatgcagaa acgactgatg acaaaattgc
      121 tgctcaagat aataaaatta gtaacttaac agcacaacaa caagaagccc aaaaacaagt
      181 tgaccaaatt caggagcaag tatcagctat tcaagctgag cagtctaact tgcaagctga
      241 aaatgataga ttacaagcag aatctaagaa actcgagggt gagattacag aactttctaa
      301 aaacattgtt tctcgtaacc aatcgttgga aaaacaagct cgtagtgctc aaacaaatgg
      361 agccgtaact agctatatca ataccattgt aaactcaaaa tcaattacag aagctatttc
      421 acgtgttgct gcaatgagtg aaatcgtatc tgcaaacaac aaaatgttag aacaacaaaa
      481 ggcagataaa aaagctattt ctgaaaaaca agtagcaaat aatgatgcta tcaatactgt
      541 aattgctaat caacaaaaat tggctgatga tgctcaagca ttgactacga aacaggcaga
      601 actaaaagct gctgaattaa gtcttgctgc tgagaaagcg acagctgaag gggaaaaagc
      661 aagtctatta gagcaaaaag cagcagctga ggcagaggct cgtgcagctg cggtagcaga
      721 agcagcttat aaagaaaaac gagctagcca acaacaatca gtacttgctt cagcaaacac
      781 taacttaaca gctcaagtgc aagcagtatc tgaatctgca gcagcacctg tccgtgcaaa
      841 agttcgtcca acatacagta caaacgcttc aagttatcca attggagaat gtacatgggg
      901 agtaaaaaca ttggcacctt gggctggaga ctactggggt aatggagcac agtgggctac
      961 aagtgcagca gcagcaggtt tccgtacagg ttcaacacct caagttggag caattgcatg
     1021 ttggaatgat ggtggatatg gtcacgtagc ggttgttaca gctgttgaat caacaacacg
     1081 tatccaagta tcagaatcaa attatgcagg taatcgtaca attggaaatc accgtggatg
     1141 gttcaatcca acaacaactt ctgaaggttt tgttacatat atttatgcag attaatttac
     1201 agagggactc gaatagagcc ctcttttcag gttttaccgt gacaatccct attaaaaatt
     1261 atatcaaaat agcttgaaaa tattggaaaa gtatggtaga atgaaaattg tcgtgtgaac
     1321 gataatactc attcttgatg aattgtgaag cagttgccct tgggtcgttt tgcgagttga
     1381 agtcaagaag aggaaaaaaa caaaaaggag aaatactcat ggcagtaatt tcaatgaaac
     1441 aacttcttga ggctggtgta cactttggtc accaaactcg tcgctggaat cctaagatgg
     1501 ctaagtacat ctttactgaa cgtaacggaa tccacgttat cgacttgcaa caaactgtaa
     1561 aatacgctga ccaagcatac gacttcatgc gtgatgcagc agctaacgat gcagttgtat
     1621 tgttcgttgg tactaagaaa caagcagctg atgcagttgc tgaagaagca gtacgttcag
     1681 gtcaatactt catcaaccac cgttggttgg gtggaactct tacaaactgg ggaacaatcc
     1741 aaaaacgtat cgctcgtttg aaagaaatta aacgtatgga agaagatgga actttcgaag
     1801 ttcttcctaa gaaagaagtt gcacttctta acaaacaacg tgcgcgtctt gaaaaattct
     1861 tgggcggtat cgaagatatg cctcgtatcc cagatgtgat gtacgtagtt gacccacata
     1921 aagagcaaat cgctgttaaa gaagctaaaa aattgggaat cccagttgta gcgatggttg
     1981 acaccaatac tgatccagat gatatcgatg taatcatccc agctaacgat gacgctatcc
     2041 gtgctgttaa attgatcaca gctaaattgg ctgacgctat tatcgaagga cgtcaaggtg
     2101 aggatgcagt agcagttgaa gcagaatttg cagcttcaga aactcaagca gattcaattg
     2161 aagaaatcgt tgaagttgta gaaggtgaca acgcttaatt tatacaaata gtaattacct
     2221 aggagggcgg ggcttagccc ggctctccta ttttcaaaaa tataggagaa ttaaaatggc
     2281 agaaattaca gctaaacttg taaaagagtt gcgtgaaaaa tctggtgccg gtgttatgga
     2341 cgctaaaaaa gcgcttgtag aaacagacgg tgacatcgaa aaagcgattg aattgcttcg
     2401 tgaaaaaggt atggctaagg cagctaagaa agctgaccgt gttgctgcag aaggtttgac
     2461 tggtgtttat gttaacggta atgttgcagt agttattgaa gtaaacgctg aaactgactt
     2521 cgttgcaaaa aacgctcaat tcgttgaatt ggtaaatact acagctaaag tcattgctga
     2581 aggaaaacct gctaacaatg aagaagctct tgctttgata atgccttcag gtgaaactct
     2641 tgaagctgca tacgtatctg caacagcaac tatcggagag aaaatctcat tccgtcgctt
     2701 tgcattgatt gaaaaaacag acgcacaaca ctttggagca taccaacata acggtggacg
     2761 tatcggtgtt atttcagttg ttgaaggtgg agacgaagca cttgctaaac aattgtcaat
     2821 gcacatcgca gcgatgaaac caacagttct ttcttacaaa gaattggatg agcaattcgt
     2881 taaagatgag ttggcacaat tgaatcacgt tatcgaccaa gacaacgaaa gccgtgcaat
     2941 ggttaataaa ccagctcttc cacacttgaa gtatggatca aaagctcaat taactgatga
     3001 tgttattgct caagctgaag ctgacatcaa agctgaattg gctgcagaag gcaaaccaga
     3061 aaaaatctgg gacaaaatta tcccaggtaa aatggatcgc ttcatgcttg ataatactaa
     3121 agttgaccaa gcttacacac ttcttgcaca agtttacatc atggatgaca gcaagacagt
     3181 tgaagcatac cttgaatcag ttaacgcttc ggtagttgag tttgctcgct ttgaagttgg
     3241 tgaaggtatc gagaaagctg caaacgactt cgaagctgaa gttgcagcta caatggcagc
     3301 agccttgaat aactaataat agaaaggaag caatttgctt ccttttttgt atagggctta
     3361 gatttttcct gaaattgttt ttcccccagt ctcactcgat tagaataatt aaaacaaaaa
     3421 gccctataat aagggctaag tgcatttagg agactatact tcaaattcat agtaagcgcg
     3481 tcataacaag gtatctatcc taattcccca acttccagta tactagataa aaaataatta
     3541 tcggaggaaa ggtattgtac tatgatgatt caactaagtg atttaggtca agttcacatt
     3601 gtttgtggca agacagatat gaggcaggga atagactcat tagcctatgt agttaaaacc
     3661 cactttgaat tggattcttt ctccggtcaa gtctttctct tttgtggtgg acgtaaagac
     3721 cgctttaaag tcctttactg ggatggtcaa ggattttggc tactatataa acgctttgag
     3781 aacggaaaac tgacttggct aagtacagaa aaggatgtca aagctctcac atctgaacaa
     3841 gtagactggc ttatgaagga cttttctatc actccccaaa tatagtggtt tgaaactaga
     3901 atagtacacc tctgcttcta aaacattgtt agaaatcgat ttgactgtcc tgttcttatt
     3961 tcattttact ataaattcat cagaaagtcg tgatttctat tgaaatgagg actttctttt
     4021 tatactcatc tgttttcaca aagcattcta gtccatttcc gattaacgat ggactttatc
     4081 acctccttct ccagtccttg tataacatct tggagttgat tcatgacatc ttacaaaatt
     4141 tgaaaggctt tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggt
     4201 tcatctctgg tgtgtatgga ggaataaagg taaaaccaat attagtcgga atctttaagg
     4261 tacttgattt atgccatata gcattgtcca taacgagtaa aaaataatca tctggatgct
     4321 cctattccta aagccccttt agcgcatagc tttggctcag cttctattat cgctcacatc
     4381 atccatcaga agtttaatct gaaggtaccc aattatcgcc aagaagaaga ttgggctaag
     4441 atgggtttac caatcacacg taaggaaatt gctaattggc atatcaaggc gagtcaatac
     4501 tatttggagc ccctttataa cctcttgcga gagagactat tgacgcagtc cttacttcat
     4561 gcggatgaaa cttcttatag ggtgctagag agtgatagtc agctgactta ctattggact
     4621 tttttgtctg ggaaagctga gaaacaaggg attacgcttt accatcatgt cctaatcgat
     4681 ttgttcatat cttatttcaa tccattataa atagtgagaa atatttatcc tatcttctag
     4741 aatgtcttcc aaacgtggaa actctcgtaa acaaagaggt tttagaggcc tatttacc
//
LOCUS       DAVMEF010000129         4770 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMEF010000129 DAVMEF010000000
VERSION     DAVMEF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.129"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(494..1045)
                     /locus_tag="VYQ30_001654"
     CDS             complement(494..1045)
                     /locus_tag="VYQ30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966728.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1444..2268)
                     /gene="nadE"
                     /locus_tag="VYQ30_001655"
     CDS             complement(1444..2268)
                     /gene="nadE"
                     /locus_tag="VYQ30_001655"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1966729.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGFHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2265..3725)
                     /locus_tag="VYQ30_001656"
     CDS             complement(2265..3725)
                     /locus_tag="VYQ30_001656"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1966730.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEGLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3841..4329)
                     /locus_tag="VYQ30_001657"
     CDS             complement(3841..4329)
                     /locus_tag="VYQ30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966731.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4319..4528)
                     /locus_tag="VYQ30_001658"
     CDS             complement(4319..4528)
                     /locus_tag="VYQ30_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1966732.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1371 a         1099 c          865 g         1435 t
ORIGIN      
        1 ctcatatata aaggattgca gacatcttat ctaaatacat gctaatatat ttagatacaa
       61 acattccaac ttgataactt tcactcatct ttcatcattc cttatacaac tatgcagtat
      121 aaatagaata gttttctcat cagaatgaga ctattttaat attagatatt ttgatagaaa
      181 aaacataaat aaaattggtt gaatttaatt ccctaactcg atagaggatt ctattttaaa
      241 tttaacaaca gctaggatta cactataaaa agcacattac cacctgttcg gctggagaat
      301 ctagtcgatt agctccctct tagttacaag cttcaagcga ttttttaact aatactaaac
      361 cagattaaag aaggaactag aacatctgtc ttgtactaat caatatgcta ttttaaagtt
      421 gcaaataaac ttggaatgct aattattata tttacaataa atagttgact tcgatagaaa
      481 cagaaaatag aattcaacat tcttccctac ttataccata ataaataaga tccgcaagat
      541 agccttttct ctctacacca ttaacaatgg tttgtagata ggacattcca gccttttcca
      601 tgacacgacc tgaagctggg ttgagactgg cataacgtgc tctgaccttt tgaaaacctg
      661 cttgagtaaa acaaaagtcc aagacagctt tcaaggcctc tgtcatcata ccatttcccc
      721 agtaagcctt gcctaacaca tagccaattt cacagcttaa atcagcctcg tctatcttaa
      781 caatgctgat atctcctatc acttgctctg ggttttcttt tagacaaatg gcccatttat
      841 aatagttgag attagtatag gaagcaaccc aattgcgaat cgagtttcga gtgatttcga
      901 tatcaggatg gggatcccag gtaacatagg tcagattctc agcggatgaa gcccaatttt
      961 gaaacatggc ttctgcatca ctctccacaa atcttcttaa aatcaaacgt tctgtctgta
     1021 atatttgcgt accgatagat ttcatgatgc tacctcactt ccttatttct atgctcgtag
     1081 taaaaaggct caactcagta acagctagaa aacgagtttg tcttctaatt tcttgacgca
     1141 gtcgctgcct ggtttgaaat gcgctctagc aagctttttc aaacctagtc atcgttgcag
     1201 gggtaagacg atgaaatcga actcttcgag ttaccgaatc tgcctcattt tcttatttct
     1261 aaactcgggg ttcaagatcc cccagacctt ctctcacttt cttatttcaa gatgagaggc
     1321 tgaaaacctc cactggaggt ttttcttgct ttttaatttc agggcaagaa gctaaaaagg
     1381 tcccccggac cttcttcgct ttctcatttc taggctcagg gtaaaaacct ccagtggagg
     1441 tttttactcc caaaagtcat caaatacagt gatgggtaag tggcgtttgt gttggccttt
     1501 gtgccaccag ttttcaatgg tcgcttgagc ttctgggctg attgttttgc cttctaggta
     1561 gtcgtcaatc tctgcgtagg tgactccaag tgcgacttcg tcagctaggc ctggtttatc
     1621 ttcttctagg tctgccgttg ggattttttc ataaagggct ggctctgcgc caagtttctg
     1681 caagagctgt tttccttggc gtttattgag gcggtaaaga gggagaatat ccgcaccgcc
     1741 gtcaccaaac ttggtaaaga aacctgtgat attttctgcg gcgtggtctg ttccaatgac
     1801 cgctccgcta tggaaaccag caagggcata ctgagcaatc atacggcaac gtgccttgat
     1861 attccccttg ttgaagtctg aaacaggact acctgtcgct tcaactgcag ctgtcatggc
     1921 atcagctgat tccttgatat tcacaaccaa gctgacatct ggctggatga aggctagggc
     1981 tttttgagca tctgcttcat cagcttgcac tccgtatggc aggcggacag cgataaattt
     2041 gtagctatcg tctcccgttt cagctcgcag ttcttccata gctaattgcg ccaaacgtcc
     2101 tgccaaggtt gagtcttgtc ccccagaaat ccctagtaca aaggttttta ggaagggatg
     2161 ttttttcaga tatcttttta agaaatcaat agaacgacgg atttcttcct gggcatcaat
     2221 cactggtttg acacccagct cttggataat cgtttcttgc aaactcattc ttcttctcct
     2281 tcaccaaggg cttccttgcg catcttatca atcaagtcca tcttatcttg ccatacgtca
     2341 cgcgccaaat ccactggata gtgctgcgga ttgagcacac gcttatactc atcccacaac
     2401 ttgtcaaatt ccttacgggc ataatcctga atgtcagtca aactaggcaa gttgtaaact
     2461 aatattcctt ctttgaagat atccaccaag agaggaacag catcaaaatt acgaaccgtc
     2521 ttcttgatgt atgtataggt cggatggaac atcttgattt ctgtcatgtc gctaatatcc
     2581 acaccatcat aagtgatgta gtcaccttct gacttgcctt tttcacgact ggtaatgcgc
     2641 cacacctgct tcttacctgg cgtcgacact ttttccgcat tattagacag cttaatcgta
     2701 ttgcgcatct ggccgttttc atcttcgatt gcaacaatct tgtaaaccgc cccaagagcc
     2761 ggctggtcat aggctgtaat cagcttggta cccacacccc agacatcaat cttggccttt
     2821 tgcatcttga ggttaaggat ggtattttca tctagatcat tagaagcata aatcttagcc
     2881 tctgtaaatc cagcctcgtc cagttgctga cggactttct tagaaatgta ggcaatatcc
     2941 ccagagtcaa tccgcacacc cataaagtta atctgatcac ccagctcacg cgccacctga
     3001 atggcagctg gtacaccgat gcgaagggta tcataggtat ccacaagaaa gacacaattt
     3061 ttgtgggtcg cagcgtaagc cttgaaagcc tcatagtcat tgccataaac ctgtaccaag
     3121 gcatgggcat gggttcccaa aacaggaatg tcaaagagct tacccgcacg cacgttgctg
     3181 gttccattgg cgccaccaat caccgctgcg cgtgttcccc agatggccgc atccatttct
     3241 tgagcccgac gtgtcccaaa ctccatcaag ggttcatctt cgataaccga acgaatacga
     3301 gctgccttcg tcgccaccaa ggtctggtag ttgacgatgt tcaaaagagc cgtttcgacc
     3361 aactgacatt gggctagagg tccttccacc tgcacaatcg gttcattagc aaaaaccaaa
     3421 tccccttctt gggcagaacg aacggtcaac tccaacttga aattgcgaag gtaatccaag
     3481 aacgccccat gataaccaag cgactccaaa taggctatat cactatctga aaaacgcaag
     3541 ccttcaagat agttcacaat tctttccaaa cctgcaaaaa ccgcatagcc gttcttaaaa
     3601 ggctgttggc ggaaatacac ctcaaagacc gccttcttat tgtgaatccc ttggtcaaag
     3661 taaacctgca tcatgttgat ctggtacaag tccgtgtgca atgtcaaact atcatctgga
     3721 tacatacttt tcctacttcc ttagctagaa acccatgaaa attttcaaga actttcatgt
     3781 attccaataa attagtacta ttatatcaca ttttagctgg attgagaaaa gagtaacaag
     3841 ctattctcca ctctccaatt catccatatc ttgttcaaat tttttctgag cccattcgcc
     3901 atagctctta agaccaagat tgccaataaa gacccacgga aggtaaatga cataagtaat
     3961 gacccaagca gacaggtatt taaaattcaa aggattgtgc tgataaattt ctatgttgaa
     4021 ttgataattc tgcaacatca aaagagccgt aatagccaag gttaggaaaa aacaacccaa
     4081 aatcgtaaaa tgaaaacgac tatagtaggt cactcccaga taacgggcac gattgaaaaa
     4141 gtaaaatgtc cctatgatga taacgattag cagcatatta gaattaaaaa ggcttggtgc
     4201 taatactgaa atgatataag ataggagcga caaagcgatg cagatataga aactttcaga
     4261 gcccgcttta ttgaacagtt gttcttctct ttcgtctagt aattgataat aataaaatct
     4321 atttttcatc ttcttcctcc caaaatagtt ggtctagggt tttccctaaa catctgcaaa
     4381 tagactggca gagcgagaga ctgggattgt attttcccgc ctctatcaaa ccaatagtct
     4441 ggcgtgtcac cccgacagcc tctgccagtt gaccttgtgt taaatcacgc tctacccgag
     4501 ctaattttaa ttttaaattt ttagccacct tcgtcctcct tatagtttta atactcatct
     4561 actcttaaaa aatccaaaac caacacaagc tatcagctat ttattataac cccattaatt
     4621 tgcactccag tattatcttc agccgaaaat cagtatttta ggttgtttga aataaacttc
     4681 ctagtttact cttttatttt tatttagtat tagctttagt aggatctttt aaacgagttt
     4741 ttattgctta tatttcttgt ttagtttagt
//
LOCUS       DAVMEF010000130         4758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMEF010000130 DAVMEF010000000
VERSION     DAVMEF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.130"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            82..678
                     /locus_tag="VYQ30_001659"
                     /pseudo
     CDS             82..678
                     /locus_tag="VYQ30_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            754..1203
                     /locus_tag="VYQ30_001660"
     CDS             754..1203
                     /locus_tag="VYQ30_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1966733.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1216..2247
                     /locus_tag="VYQ30_001661"
     CDS             1216..2247
                     /locus_tag="VYQ30_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966734.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     IAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEPLSNLEAKLRLDMRQAIREIQ
                     HEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTN
                     IIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHVSIRPEEFIKDESGDIEGTIS
                     DSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNRIRLRINTQKLNIFSADGSQN
                     LIKGVNHGT"
     gene            2237..3882
                     /locus_tag="VYQ30_001662"
                     /pseudo
     CDS             2237..3882
                     /locus_tag="VYQ30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3985..4677)
                     /locus_tag="VYQ30_001663"
     CDS             complement(3985..4677)
                     /locus_tag="VYQ30_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1966735.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1475 a         1022 c          824 g         1437 t
ORIGIN      
        1 actatagaat agaattgtag gttttaaaaa cgcttacatt gttttaaaat aaacttaaat
       61 aacacaacgg aggtatccat catgaaaaag aaatggatgt attatgctgc ttgttcttct
      121 aatgaatctg ccgatgacag ttcatctgat aaaggagacg gtggttcgct agtcgtttat
      181 tcaccaaact cagaaggctt aattggagca actattcctg cctttgaaga aaaatatggt
      241 atcaaaatag aactgattca agctggtact ggagaacttt tcaaaaaact agagtcagaa
      301 aaagaagttc ctgtagctga tgttaccttt ggtggttctt atacacaata tgctacccac
      361 ggagaactct ttgaaaacta tatttcaaaa gaaaatgata atgttatcaa agaatatcaa
      421 aacacaactg gcttctctac tccttataca ctagatggta gtgttttaat cgtccaccct
      481 gatttaacta aaggcatgaa catcgaagga tataacgatc ttctcaaacc tgaactaaaa
      541 gaaaaaatcg caactgctga cccagcaaac tcttctagcg cctttgctca attaacaaat
      601 atgctacaag ctcaaggtgg ttacaaagat gataaggctt ggtcttatgt aaaagatctt
      661 ttcacactta ttgatggtat agtaaaatga aataagaaca ggacaaatta atcaggacag
      721 tcaaatcgat ttctaacaat attttagaag tagatgtgta ctattctagt ttcaatatat
      781 tataaaatcg gtttaagttc atctggtgtc tataaagcag tcgctgatgg agaaatgact
      841 gttggtctct cttatgaaga tccagcagtt aaactcttaa atgacggagc taacattaag
      901 gtagtctatc caaaagaagg aaccgtcttc ctacctgcta gtgctgctat cgttaaaaaa
      961 tctaaaaata tggaaaatgc caagaaattt atcgatttta ttatctctca agaagtacaa
     1021 gatacacttg gtacaaccac tactaaccgt cctgttcgta aaaatgctaa aacaagcgaa
     1081 aacatgaaac caattgacaa aatcaaaaca ctcactgaag attatgatta tgtcatcaag
     1141 aataaatcag atatcgttaa gaaatacaac gaagtcttta cagatatcca atctaaacag
     1201 taaaagaggt tcactatgag tgagatcgaa attattaacg ccaaaaaaat ctaccacgat
     1261 gtccctgtta ttgagaattt gaacattaca attccaaaag gaagtctctt tacccttctt
     1321 ggagcttcag gatgtgggaa aacgaccctt cttcgtatga ttgcaggttt caacagtatc
     1381 aaagatggag aattttactt cgatgataca aaaatcaata atatggaacc cagcaaacgc
     1441 aatatcggga tggttttcca aaactacgct attttcccac atttgactgt ccgagacaac
     1501 gttgcttttg gtcttatgca aattgctcaa tatgcggatc gaaagcccga taaactcagt
     1561 ggtggacaac aacaacgtgt caccttggca tgcgccttag cggttaatct aagtgttctc
     1621 ctcatggacg agccacttag taatctggag gccaaacttc gcttggatat gcgtcaagcc
     1681 atccgagaaa tccaacacga agtgggaatt acaactgttt atgtaaccca cgaccaagaa
     1741 gaagccatgg ctatttcaga ccaaattgct gttatgaaag atggggtgat ccaacaaatc
     1801 ggccgaccaa aagaactcta tcataaacca gctaatgagt ttgtggcaac ctttatcgga
     1861 cgcacaaata ttatccctgc caatcttgaa aaacggagcg acggcgctta tatcgtcttt
     1921 tcagatggct atgcccttcg aatgctagct cttgatcagg ttgaggagca agctattcat
     1981 gtaagcattc gtcccgaaga gtttatcaaa gatgaatctg gagatattga aggaactatt
     2041 agcgatagcg tctatcttgg actaaatacg gagtatttca ttgagacagg ttttgcctca
     2101 aaaattcaag ttagtgaaga atcaactttt gaagaagatc tacaaaaagg caatcgtatt
     2161 cgtctacgaa tcaatacgca aaaattaaac atcttttctg cagatggttc ccaaaacctg
     2221 ataaaaggag tcaaccatgg aacgtaaaaa actaaatatt tggacagcct cctctttctt
     2281 catctttctt acctatcttg tctttctcgt ttatcctatc gttaccgtgc tcaagtaagc
     2341 acttatacat gaaggacaat tctcactagc taattttgtc actttcttta gtaaagtcta
     2401 ctactctgag acactagtca acagtttcaa ggtttccatt accgctactg tcacttcctt
     2461 agttgtagga accctattag cttatctctt ctctatgtat gacttcaagg gggagaaatt
     2521 tctacaaata ttgattatca ttgcttccat gtcagctcct ttcgtaggag cctactcctg
     2581 ggttctcttg ctaggacgaa atggggtcat cactaaattt ttgacaaatg cccttcatct
     2641 tccagctatc gatatttatg gattcaaagg aattgtactt gtctttacac tgcaactatt
     2701 cccactggta tttctatatg ttgctggaac aatgaacagt attgacaatt ctctacttga
     2761 agcagctgaa agcatggggt ccttcggatt taaacctatc gtaacggttg ttttacctct
     2821 tctagttcca accttactag cagctccttg cttgtattta tgagagcatt ctcagacttt
     2881 ggaacgccta tgttgattgg cgaaggatat cggactttcc ctgtcctgat ttatacccaa
     2941 tttattagcg aggttggagg aaattctgct tttgcaatta tggcgattat cattgccttg
     3001 gcaattttcc ttatccaaaa acacattgca aaccgctaca gtttcagcat gaatctgatc
     3061 catccaattg agcctaaaaa aactacaaaa ggaaaaatag ctgccattta tgcaacagtc
     3121 tacggaatta tctttatctc tgttttacct caaatctact taatttatac ctctttccta
     3181 aaaacatcag gtatggtatc tgttaaaggt tattctccaa acagttacaa ggtagctttc
     3241 catcgtatgg gatctgctat tttcaatacc attcgtatcc ctttgattgc cttagttcta
     3301 gttgttctat ttgcgacatt tatctcctac ctagccgtta gaaaacggaa tttgtttaca
     3361 aacttaattg acagcctcag tatggtacct tatattgtac caggaaccgt tctagggatt
     3421 gccttcattt cttccttcaa tactggtcta tttggaagtg gatttcttat gattacaggg
     3481 actgctttca tcttgattat gtctctatct gtcagaagat taccttatac tattcgctca
     3541 tctgttgcta gcttacaaca aatagcacca agtattgaag aagctgctga aagcttagga
     3601 agtagtcgtc tcaatacctt tgctaagatt acaagtccaa tgatgctatc tggtatcatt
     3661 tctggagcca tcttatcttg ggtcacaatg atttcaaaac tctctacttc tatcctcctc
     3721 tacaatgtca aaacaagaac aatgactgta gctatttata cagaggttct cagaggaaat
     3781 tacggtgtag ccgcagcctt gtcaactatc ctgactgttc taacagtagg ttccttgctc
     3841 ttgtttatga aaatctctaa aagcaatagc attacacttt agttttccac atcaaaaaca
     3901 gccgaaagga gtaccctcgg ctgttttttc ttatcttgat attcttatca aatccctagc
     3961 tcatggcaag caagtctaga ctgattaaat agagccttct tccatcttct cacgtcctag
     4021 ctctcggtaa ctacggtcac cgacaacttc aaccctaaac tgaccgtcct catattcaag
     4081 aatcgtcacg ctaccattat ctagaccatg cggatgcatg ccattaatca gataaacaat
     4141 ggttccaata gtcattccat ggctgacaac aagggcgtta cctccacctt gatcttccat
     4201 ttcttttgca atcatttcaa agccttcctt gattcggcca ctgagttttt cccagccttc
     4261 agcccaacca gctgtatcga cctctaccaa gccctcagcc agttcagcat aagacaattg
     4321 gtgaacgtgg tccacattaa agatacgagg aataatgccc atgaaaagat cgccatcata
     4381 ggctccatca aaactaccga aacaccattc tctaatacgc ttgtccatgc gataagggat
     4441 tttcccctgc aagccaagtt cttcaaggat aattcccatg gtctgaatgg tacgaccaga
     4501 atcactcgaa taagcgcgct caaactgtag atcagattct cgcaaaccga ttcctaactc
     4561 ttgaatccct cgttcacctt cagcagttaa gggagtatcg ctccaacctt gcgcgcgacc
     4621 aatcgtgtta aacatggtct tgccatgacg taccaaatac aatcgtactt ttaccatttt
     4681 ccgtcctccg tttgcttcta ttatatcatg aatgataaaa taaaagccac ccatacaggc
     4741 gacttttgaa ggagatta
//
LOCUS       DAVMEF010000131         4674 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMEF010000131 DAVMEF010000000
VERSION     DAVMEF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.131"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            111..791
                     /locus_tag="VYQ30_001664"
     CDS             111..791
                     /locus_tag="VYQ30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966736.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWRILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVELPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            751..1131
                     /locus_tag="VYQ30_001665"
     CDS             751..1131
                     /locus_tag="VYQ30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1966737.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            1141..1428
                     /locus_tag="VYQ30_001666"
     CDS             1141..1428
                     /locus_tag="VYQ30_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966738.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            1429..2187
                     /locus_tag="VYQ30_001667"
     CDS             1429..2187
                     /locus_tag="VYQ30_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1966739.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGKVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2199..3065
                     /locus_tag="VYQ30_001668"
     CDS             2199..3065
                     /locus_tag="VYQ30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966740.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGPSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPTTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKRF
                     EVGITSLGDPDTIAATVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(3152..3799)
                     /locus_tag="VYQ30_001669"
     CDS             complement(3152..3799)
                     /locus_tag="VYQ30_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966741.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3796..4494)
                     /locus_tag="VYQ30_001670"
     CDS             complement(3796..4494)
                     /locus_tag="VYQ30_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966742.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
BASE COUNT         1471 a         1007 c          799 g         1397 t
ORIGIN      
        1 ttatgtctat ggtaaaaaat ccttataacg gcaataaaaa atagatatta tccaaagaag
       61 attttaagtg ctacaataac tgtattattt ctagatggga ggttctattt ttggattggt
      121 ccattgttga acaatatcta ccactatatc aaaaggcatt ctttctgacc ttgcatattg
      181 cagtttggag aattttggga tcctttctgc tcggtttaat cgttagtatc atccgacatt
      241 atcgaatccc tgttttggcg caagtagcga cagcctacat tgaattgtca cgtaatacgc
      301 cccttttgat tcaactcttc tttctctact tcggtcttcc ccgaatcggg attgtcctat
      361 cttcagaagt ctgtgcaacg cttgggcttg tctttttagg aggctcctat atggcagaat
      421 ctttccgaag tgggctggaa gccatcagtc aaacccagca ggagattggc ctcgctattg
      481 gtctgacacc tctacaggtc tttcgctatg tggttcttcc gcaagcaaca gcggtggaac
      541 tcccctcctt tagtgccaat gtcattttcc ttatcaagga aacctctgtt ttctcagcag
      601 tggctttggc cgacctcatg tacgtcgcca aggatttgat tggtctctac tatgagacag
      661 acattgcgct agctatgttg gtagttgctt atctgatcat gctgctaccc atctcactgg
      721 tctttagctg gatagaaagg aggacccgcc atgcaggatt cgggaatcca agtactcttt
      781 caaggaaata atctcctgag aatcttacag gggttgggcg ttacgattgg gatatccatc
      841 ctgtctgtcc tcttatccat gatgttcgga acagtcatgg gaatcatcat gacctcccat
      901 tctagaatca tacgattttt aacacgattg tatctggaat ttatccgtat catgccccag
      961 ctggtgctac tcttcatcgt ttactttggc ttggctcgaa actttaatat caatatctca
     1021 ggtgagactt cagctattat catttttacc ctctggggaa cagctgaaat gggagacttg
     1081 gtacgtggag ctatcacttc tctccctaaa catcagtttg aaagtggata ggcactcggc
     1141 ttgactaatg ttcaacttta ctaccacatc atcatcccac aagtcttaag aagactgcta
     1201 ccgcaggcta tcaatcttgt cactcggatg attaaaacca cttcattagt tgttttgatt
     1261 ggggttgtgg aagtgaccaa agttggacaa caaatcatcg atagcaatcg cctgaccatc
     1321 ccaactgctt cattttggat ttatggaacc attctagtct tatatttcgc agtttgctac
     1381 cctatttcca aactatccac tcacttagaa aaacattgga gaaactaaat gtctgaaact
     1441 atcttagaaa tcaaggaact aaaaaaatcc ttcggagaca atcccatcct ccaaggactt
     1501 tctctagaaa tcaaaaaagg gaaagttgtt gtcatcctag ggccatctgg ttgtgggaaa
     1561 agtaccctcc ttcgttgcct caacggctta gaaagtattc aaggtggaga tatttttctg
     1621 gatggtcagt ctatcgttga aaataaaaaa gattttcacc tagttcgcca aaagattggc
     1681 atggtctttc aaagttatga actctttccc catctggatg tcttacaaaa cctcatccta
     1741 ggccctatca aagctcaagg aagggacaag aaagaagtaa cggaagaagc tttgcaatta
     1801 ctagagcgtg tcggtttgct ggataaacaa catagctttg cccgtcaatt atctggtgga
     1861 cagaagcaac gtgttgcaat tgtccgtgcc ctcctaatgc atccagaaat catccttttt
     1921 gacgaggtga ctgcttcgct ggatccagaa atggtgcgtg aggtgctgga acttatcaat
     1981 gatttggccc aagaaggccg taccatgatt ttagtaaccc acgaaatgca gtttgcccaa
     2041 gccattgctg accggattat cttcctcgac caagggaaaa tcgctgaaga aggaacagct
     2101 caagccttct ttaccaatcc gcaaaccaaa cgagcccagg aatttttaaa cgtctttgac
     2161 tttagccaat tcggctcata tctataaagg agattcttat gaaactattc aaaccactct
     2221 taactgtttt agcacttgcc tttgccctta tctttataac tgcttgtagc tcaggtggaa
     2281 acgctggtcc atcctctgga aaaacaactg ccaaagctcg cactatcgat gaaatcaaaa
     2341 aaagcggtga actgcgaatc gccgtgtttg gagataaaaa accgtttggc tacgttgaca
     2401 atgatggttc ttaccaaggc tacgatattg aactagggaa ccaactagct caagaccttg
     2461 gtgtcaaggt taaatacatt tcagtcgatg ctgccaaccg tgcggaatac ttgatttcaa
     2521 acaaggtaga tattactctt gctaacttta cagtaactga cgaacgtaag aaacaagttg
     2581 attttgccct tccatatatg aaagtttctc tgggtgtcgt atcacctacg actggtctca
     2641 ttacagacgt caaacaactt gaaggtaaaa ccttaattgt cacaaaagga acgactgctg
     2701 agacttattt tgaaaagaat catccagaaa tcaaactcca aaaatacgac caatacagtg
     2761 actcttacca agctcttctt gacggacgtg gagatgcctt ttcaactgat aatacggaag
     2821 ttctagcttg ggcgcttgaa aataaaagat ttgaagtagg aattacttcc ctcggtgatc
     2881 ccgataccat tgcggcaaca gttcaaaaag gcaaccaaga attgctagac ttcatcaata
     2941 aagatattga aaaattaggc aaggaaaact tcttccacaa ggcctatgaa aaaacacttc
     3001 acccaaccta cggtgacgct gctaaagcag atgacctggt tgttgaaggt ggaaaagttg
     3061 attagtcatt aactcttaaa aggaactgga ttttaagctc caatcccttt ttaagatttt
     3121 acctataaca tcctgagtct atctaagatg ttcaatctga acacaatgta catactttat
     3181 cttctattgc atatacttta tcacataaga tacgaatatc ctcttcacta tgactagcaa
     3241 tcaaaattgt tgtccctttt tcactagaga gctttctaaa caatgttctc atattttcta
     3301 cacttgattt atccaaggca ttcataggtt catctagtaa aagaatagag ggattctcca
     3361 tgattgcttg agcaatccct agctttttcc tcatacctag cgaataagtt ttaactttct
     3421 ggtctttttg ctcatataga ccaactattt tcagtgtatc attgatttcc tgattaccaa
     3481 ctactcctct tatgcttgcc aaatattgta aattcttaaa gccactataa taatttataa
     3541 aaccaggttc ttcaatcaaa gctcccaaat tagctggaat ttttctctca ggaacaatat
     3601 tttccccatt gattaacact tctccataag acggactata taaaccagct attaatttaa
     3661 acaatacact tttccctgag ccattcgcac cagtaattcc tataatttcc ccctgtttac
     3721 aactaaaatt aaggttttga aaaacacatg tcttttttaa tttcaactca atatttttta
     3781 atgtaattat ttcattcatt ctataaacct cctcttttga cgagtgaaat agaaaatgct
     3841 ttgaaaaaga aagactaaaa atagcaactg aagaaataaa tctcgtccta tatctccatt
     3901 ccctcgattc aaaatataaa atagataatt agttcgattt cctacaaata gaccaccaaa
     3961 cacaatcatg agtaaaaaga aactaacgca agcaaagttc atttgtacat tcgctcctag
     4021 tgcgtataac ataaagcaaa gcaaggatag cacacaagca ttcaaagaat caactataaa
     4081 caaaactcct atatcccagt ttggtaactg taacataaat tgaatcacta gaacttttac
     4141 catgattaaa atgagtccat aaagaaaaca ggtccaaatc tgatacctaa tccagagctt
     4201 aatagaagaa aatcgaatca cttccagaaa gaaatcggat gctcttctac gatatacaac
     4261 tgctagaaaa aatgtatgaa taataaacca atgaagcaac catatagata agactaaaaa
     4321 agagttttga ctcagattag gcccttttaa aatttctata acaagtctat catttccttc
     4381 taaccaatac cataatccta aacaaatcaa tatccaaaaa agaacataac cccaaaagta
     4441 cttggtatag attatctgat tcaacctcca catgtgacta cttaactttc ccatttccaa
     4501 cttttacact ctcctccagt ttctatgaac agaaaataca gtcaatggaa ttatgataga
     4561 tagtaatgca agccaataaa tcaatgcata cggattggaa tttaccatat actgaaaaat
     4621 tggcaataat ccaattatcc cattgtccaa ctgataaatc cagaataagg attc
//
LOCUS       DAVMEF010000132         4659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMEF010000132 DAVMEF010000000
VERSION     DAVMEF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.132"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(140..781)
                     /locus_tag="VYQ30_001671"
     CDS             complement(140..781)
                     /locus_tag="VYQ30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966743.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(778..2886)
                     /locus_tag="VYQ30_001672"
     CDS             complement(778..2886)
                     /locus_tag="VYQ30_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1966744.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(2938..3225)
                     /locus_tag="VYQ30_001673"
     CDS             complement(2938..3225)
                     /locus_tag="VYQ30_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1966745.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            3649..3969
                     /locus_tag="VYQ30_001674"
     CDS             3649..3969
                     /locus_tag="VYQ30_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1966746.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(4021..4608)
                     /locus_tag="VYQ30_001675"
     CDS             complement(4021..4608)
                     /locus_tag="VYQ30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1966747.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT         1467 a         1002 c          776 g         1414 t
ORIGIN      
        1 aagcctaaat tgaaaaaatt ttcaatttga aacaaaaaaa acctagaaat actacataaa
       61 atgtcattcc taggcgtatt tatgctattt ttctctggaa aacatgagta ttcagccagt
      121 caaaaggagc tgaatgaact cattttccct cgcccaattc aatgacacga tgacattgtt
      181 gggctacata agcatcgtgg gtcacaataa tgactgtttt cccttctcga ttcatctcta
      241 agagaaactt caagaccaaa tctctatttt caggatccag cgaacctgta ggttcatcgg
      301 ctaaaatcag ctggctgggt tttaagatgg ctctagcaac tgcaattcgt tgttgttcgc
      361 ccccagacaa ctcggagacc ctttgatgca aagtagctga caaacctact ctctctaaaa
      421 tctcttccac ctttttgagc ttgtctttct taggcaattt cacatatttc agcgccagca
      481 tgagattgta ctcgaccgtt tcatcatcaa tcagggcaaa attttgaaac agataagaga
      541 tatgttcacg gattattgtt tgcgacttag cagaattaac cgctagattt gtctgaccaa
      601 aaatctcata ccgtccgcta taatcaccat ctatcaaacc caataaattt aacaaggtcg
      661 acttcccact accactctta ccaacaatag ctaccaaatc cccctgatca atcctgagag
      721 ataagttatc caaaatcact tttcccccaa tggttttggt aatatttttc aactcaatca
      781 taagatgccc cctttcaata actctactag acttcttttc tccatcctag aagctaagcc
      841 tagcacaaat agtatatcca gacatgtaaa acctgcaaac agtagaagtg gtaagaacgc
      901 atgggcaaag aaaatcaaga ctagaagagg gaaactatag cccagcaaga gcagaacgag
      961 gagaggacgg tagcgatcga ccagtttcca ccccataaac ttcttggtaa tgatatccct
     1021 gcgcttcaat aagaaagttg ttactagtaa gaagtaggaa atcatcatgc taaggagacc
     1081 aaacaaagca aagagtaggt taaaattccg aacagcatct cgataagaat ccactttctc
     1141 ttgttgaatg gcttgaatag atgaaaattt taaataattt ccatctgaca atttctcaac
     1201 taactctgta atctcttttt gatgttgaac cgtattttca attttaatcg gattatttaa
     1261 gccagttgtt gacagggagg ctttctcatc ccacatcata tcagaatcat tgaccaagct
     1321 aataattgga ttggagagat tttcctttcg cttatcacta tatgggaaaa atgaccaatc
     1381 tccttcataa taggcaatct cgacatccat ctcctctatc gttcgttttt gctgctcttc
     1441 atacttcatc gaatgaaagg caattaactt ccccaagagc tgatttttat cttcttcacc
     1501 tttcgtactt gctggcatca aaataacttt tttaataccg gtatctggta gcttgaatcc
     1561 cttgctcttt agaaaattgc gattggcata gtaaacatcc accgtatctg ttaactgata
     1621 ttgctgaatc tgttctgatt ggacaaaatt ttttacagga agactgctac tctgcacata
     1681 gcccgcctgc gttttttcta ccaaatcctg ataaaatcga tagaaataat ctgtagattt
     1741 ccctgaccct gctagctctt cttgccacag attatcattg agtttgaagg tttctaaggt
     1801 caggtaatta ccttgactta cccactgttg ctgataagca agttctttgt tttcttgttc
     1861 taaacttctg cccaccccaa tcagtaaggc cgtcagtaaa atagttgtcc ctattttcat
     1921 cacataattg aagataagac caaatttgaa agatgaaaaa cctttcagca gagagctgat
     1981 tgtcattttt tggattaaga ggtaagtcaa ccaactgata aagagataaa gctgcaacag
     2041 caaaaaatga gacaaccaca gcataggaaa caaatctttt ggcttataat caagcaagaa
     2101 aaacacgcct agattgatca caagagcccc acctaggagg aggtaaaggt tgccttttac
     2161 aacatcagct aaaacagccc tatcttgaaa accaagtaat ttttgtaccc caactctttt
     2221 catctccatc atcggttgat acactgtcac taacacaaga agcaaaatag ccaagacaaa
     2281 aacaatggca gataaaagca aatctcgatt tatgacttcc actgcacttt tgtaggtcgg
     2341 ctctagcaag gtagcctggt ctatcttgaa aaaatcgctc catttctgta caatcctatc
     2401 cttgtccatc tcttgtgtag aagttatcgt atagcgacca tttaaactac gagatgtatc
     2461 cttgatatag gtttgaaaag tcataagctg aataggtttg gcttttagaa aggtcggaat
     2521 cgtaccaagt ttattggaaa tttctttatt actatagact ccttcaccat ctgtggtaaa
     2581 atcaagagaa gaaatcccaa actcttggta ggggaaggta tctttatcaa aaacaccaga
     2641 cttgaccacc tcatcaccac tgtctgtttt gatgatggag actttatact cctttgatac
     2701 atcctcaaaa aatcgaagaa cagacgctgc aggttcgtta atatctttca aatacaaatc
     2761 caaagaatct acagtctttc ccatttcttg aatccgaacc acttctcgtg tatagtttac
     2821 attaaaaaca aaaaaagtgg tacacagaag caggagtaac atacagaaat tactgatttt
     2881 tttcataaaa gtgcctccac tacagttttc cctctccaaa taggagaggg aagaatttta
     2941 ctcaaatcca tagaaatatt ccattcctgt tggcggaatc ttggtgaggg aagctctagc
     3001 ccatgcctca ggttttgcat aatccttaga ggttctaccc ccatgtctaa cagttgctgt
     3061 atgatatcga gtagaatgta agtaatcaga atatccaaaa gttcctgtcc aacctactcc
     3121 atagttccat tgaccaccct caacccatac tgctaaagca tttgtagtag caagtatacc
     3181 acataccatt aaagatgcaa caccaatttt aactaatttt ctcatgattt atttctccta
     3241 ttatcgagag ctgttccgta aaaacagcta tagtaattta tcaacaagta tcgtagtcca
     3301 ttggactcac cttccttcta tctcatttca tttttaacaa cgaacttact cttaaccggg
     3361 agaatggtgc gcttctgtat caggtccttg aacaaccaga gtagtattaa gcacactcta
     3421 actattactc aagaatcagt ccaataaaca atatggatag cgctttcaaa tttttaaact
     3481 cctatcccat ccttttatct atataataag tgaaaatata ataactgtca agtaactgaa
     3541 gtgaatttta taaaaaaatt acaagccaaa tttgtaaagt ttacactaag ccgctagggc
     3601 aatcgtctat cagaatatcc gtttatttgt caataatccg agaaaatctt gcaacgctta
     3661 gaagtctata aaaactatca acatttatat gacttgcgaa tagcaatcct gctaaacctt
     3721 tccacactct atctatacaa tcaagataaa aacatgtgta agcaaatctg ctacacttta
     3781 ctggaggacg ccaagaataa gaaaagctac gataggctgg ctatctgcta tgtccctatt
     3841 gggatttgta gggatgatgc gaaacttatc caaaaaggtt tctcactcct agagctaacc
     3901 gaggaaactt ctatgctgtc tcatctcaaa aaagaagtag agatctatta ccaagcgaag
     3961 gaaagataaa aaagccgagg gatttcctcg actctttaca tattatttgt tcgttagttc
     4021 ttaataccag ccattgttaa gccagaagtt tttagcagct gtccatgaac cgtaacgtcc
     4081 tgcaacgtag gcatctgcta cacgttcttg gttttcagct gagtagtcac cgttcaggta
     4141 tgaatctgtt aattggtaac gtccgatata acgtccattt gtagctgtat agctaccacc
     4201 tgattctttt tgagcgatcc attctttggc ttctgcttca gatccgctta cagttgaaac
     4261 aactgtttca cttactactg gagtttctgc cactggagct gaaacagttt catcttgagc
     4321 ggctggtgcc gcataagtag ctggcgctgg tgttgcaaca ggcgctacag ggccatcgat
     4381 aaccaactct tgatcaacat aaatcaaatg aatgttatca atgtggttgt tttctgccaa
     4441 tttttccact gttgtgttgt gagtttcagc gatttctgaa agtgtatcac cttctttaac
     4501 agtgtaagtt gatgattctt gagcagatac aaatgatgga gcaaatactg caaacaaggc
     4561 agctactcct gcaagagttg ctttaatctt tttagttatt gatttcatat tcgaaaattc
     4621 tccttctttc tatagttcta tcataccctt taaatatta
//
LOCUS       DAVMEF010000133         4633 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMEF010000133 DAVMEF010000000
VERSION     DAVMEF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.133"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            116..874
                     /locus_tag="VYQ30_001676"
     CDS             116..874
                     /locus_tag="VYQ30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1966748.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVIVLLGPSGSGKST
                     LIRTINVLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHETGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            885..1679
                     /locus_tag="VYQ30_001677"
     CDS             885..1679
                     /locus_tag="VYQ30_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1966749.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDGFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1692..2369
                     /locus_tag="VYQ30_001678"
     CDS             1692..2369
                     /locus_tag="VYQ30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966750.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISALRLIILPQAFRIILLPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2379..3038
                     /locus_tag="VYQ30_001679"
     CDS             2379..3038
                     /locus_tag="VYQ30_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966751.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3333..4523
                     /locus_tag="VYQ30_001680"
     CDS             3333..4523
                     /locus_tag="VYQ30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1966752.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGNKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         1312 a         1078 c          835 g         1408 t
ORIGIN      
        1 gatcgatttg tcctgttctt atttcactat agtgaatttt ttcagatatg atttgacaag
       61 aacattcttt tattgtagaa ttatgtcata aaatctaaca caagggagag ctctcatggc
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac tccgcaacat
      181 caatctccgt tttgaaaaag gacaagttat tgtcctgctt ggaccttctg gctctgggaa
      241 gtccactctt atccgtacga tcaatgtttt agaggctgtt gacaaaggaa gtctcctagt
      301 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt tttcaacatt ttaaccttta tccacacaaa acagtgttag aaaacgtgac
      421 acttgcgccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgcccaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcatgatg ggatgaacat gatcatcgtt acccacgaaa cgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
      781 agatgtcgat aacttttttg acaatccaag caaacctcgt gcccaacaat tcctcagcaa
      841 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaaaattct
      901 ttttatcagc attattgatt agccttttcg gccttgctgc tgccaaacca gtccaggctg
      961 atacaagtat cgcagacatt caaaaaagag gcgaactggt tgtcggtgtc aaacaagacg
     1021 ttcccaattt tggttacaaa gatcccaaga ccggtactta ttctggtatc gaaaccgact
     1081 tggccaagat ggtagctgat gaactcaagg tcaagattcg ctatgtgccg gttacagcac
     1141 aaacccgcgg ccccctccta gacaatgaac agggcgatat ggatatcgcg acctttacca
     1201 tcacggacga acgcaaaaaa ctctacaact ttaccagtcc ctactacaca gacgcttctg
     1261 gatttttggt caataaatct gccaaaatca aaaagattga ggacctaaac ggcaaaacta
     1321 tcggagtcgc ccaaggttct atcacccaac gcctgattac tgaactgggt aaaaagaaag
     1381 gtctgaagtt taaattcgtc gaacttggtt cctacccaga attgattact tcccttcacg
     1441 ctcatcgtat cgatgccttt tccgttgacc gctctattct atctggctac actagtaaac
     1501 ggacagcact actagatgat ggtttcaagc catctgacta cggtattgtt accaagaaat
     1561 caaatacaga gctcaacgac tatcttgata acttggttac taaatggagc aaggatggta
     1621 gtttgcagaa actttatgac cgttacaagc tcaaaccatc tagtcatacc gcagattaag
     1681 gaggataccc tatgacagat ttatcatctt ggacagccta ttttcaggat tttggacaat
     1741 ttttcaatgg tttcctcttt actcttgccc tagcgattgg atcctttatc ctcgccatgg
     1801 tcttaggaat cttctttggt gccatgtcaa ccagtaaacg tccaatttta cgcattttag
     1861 ctcgcatctt tgttgaattt taccaaaata ctcccctctt ggtgcagttt gttattgttt
     1921 tttatggcct acctcttatc agtgaccata tcatcatgat tccaatttat tggacagccg
     1981 ttctctgcgt gggactctat cacggcgctt atatcgctga ggttattcgt tcagggattc
     2041 agtctattcc tagtggtcag atggaggccg ccttgtcgca aggttttacc tatatcagtg
     2101 ccctgcgctt gattatcttg cctcaggcct tccgcatcat tctccttcca ttgaccaacc
     2161 aaattgttaa cctcatcaag aacacctcta ctgtagctat catctctgga gtagacttga
     2221 tgtttgtgac taagtcttgg tcggctctca acggaaacta tatcccaggc tttttaggtg
     2281 ctgctcttct ctactttgcc ctatgcttcc ctgttgccca gtttggtcgc aagatggagc
     2341 aagccaacaa aaaagcctat tcactttagg aggttactat ggaatctatt ttagaagttt
     2401 taaccccaga taacctagtc tttatcttta aaggatttgg cttgaccctc tatatttctc
     2461 taattgccat catcctctct actatcatcg gtaccgtgct tgccgtcatg cgaaatggaa
     2521 aaaatcctat cttacgcatt atttccagta tttatatcga gtttgtgcgc aacgttccca
     2581 accttctctg gatttttact atctttttgg tgttcaaaat gaagtccaca ccagcaggta
     2641 ttacagcctt tactctcttt acatcagcag ccttggctga gattattcga ggcggtctca
     2701 atgccgtaga caagggacaa tacgaagcag gaatgtcaca aggcttcacc tcagcccaaa
     2761 tcctctacta catcattctc ccacaagcca tccgcaaaat gctaccagcc atcatttctc
     2821 agtttgttac cgtgattaag gataccagtc tcctctactc tgttatcgcc ctacaagaac
     2881 tctttggagc cagccaaatt ctcatgggcc gttatttcga accagagcag gtcttcagtc
     2941 tttacatcct gattgccctc atctacttca gctttaacct agcaatttct agcctgtctc
     3001 atatgctagc caaacgttgg caacaagctg cagaataata ctcttcgaaa atctcttcaa
     3061 accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc tatccacaac
     3121 ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
     3181 tgagtataaa cagccactct ccagttcaat tggagagttt tttgatgata gtagatattt
     3241 atagacctca atttgacacc atacattttc tatgataaaa tgtaacaaat ttatttacaa
     3301 caaatcgaaa tctaaatgaa atggaatttt ctatgaaatc agtcaaagga ctactcttta
     3361 tcatagctag ttttatcttg actcttttga cttggatgaa cacttctccc caattcatga
     3421 ttccaggact agctttaaca agcctatctc tgacttttat cctagccact cgtctcccac
     3481 tactagaaag ctggtttcac ggtttggaga aggtctacac cgtccacaaa ttcacagcct
     3541 ttctctcaat catcctacta atctttcata actttagtat gggcggtttg tggggctctc
     3601 gcttagctgc tcagtttggt aatcttgcca tctatatctt tgccagcatc atccttgtcg
     3661 cctatttagg caaatacatc caatacgaag cttggcgatg gattcaccgc ctggtttacc
     3721 tagcctatat tttaggactc tttcacatct acatgataat gggcaatcgt ctccttacat
     3781 ttaatcttct aagttttctt gttggtagct atgccctttt aggcttacta gctggttttt
     3841 atatcatttt tctatatcaa aagatttcct tcccctatct agggaaaatt acccatctca
     3901 aacgcttaaa tcacgatact agagaaattc aaatccatct tagcagacct ttcaactatc
     3961 aatcaggaca atttgccttt ctaaagattt tccaagaagg ctttgaaagt gctccgcatc
     4021 ccttttctat ctcaggaggt catggtcaaa ctctttactt tactgttaaa aattcaggcg
     4081 accataccaa taatatctat gataatcttc aagccggcaa caaagtaacc ctagacagag
     4141 cttacggaca catgatcata gaagaaggac gagaaaatca ggtttggatt gctggaggta
     4201 ttgggatcac ccccttcatc tcttacatcc gtgaacatcc tattttagat aaacaggttc
     4261 acttctacta tagcttccgt ggagatgaaa atgcagtcta cctagattta ctccgtaact
     4321 atgctcagaa aaatcctaat tttgaactcc atctaatcga cagtacgaaa gacggctatc
     4381 ttaattttga acaaaaagaa gtgcccgaac atgcaaccgt ctatatgtgt ggtcctattt
     4441 ctatgatgaa ggcacttgcc aaacagatta agaaacaaaa tccaaaaaca gagcttattt
     4501 acgaaggatt caagttcaaa taagtaatca tactctcctg gaatttccca ggagagtttt
     4561 ttatagccaa atgaaataag acaggaacga tggaattttg gcattcaaat caattgataa
     4621 gaatgtttta tag
//
LOCUS       DAVMEF010000134         4530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMEF010000134 DAVMEF010000000
VERSION     DAVMEF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.134"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(28..99)
                     /locus_tag="VYQ30_001681"
     tRNA            complement(28..99)
                     /locus_tag="VYQ30_001681"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Arg,seq:cct)
     gene            complement(142..489)
                     /gene="rplS"
                     /locus_tag="VYQ30_001682"
     CDS             complement(142..489)
                     /gene="rplS"
                     /locus_tag="VYQ30_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1966753.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(608..937)
                     /gene="crcB"
                     /locus_tag="VYQ30_001683"
     CDS             complement(608..937)
                     /gene="crcB"
                     /locus_tag="VYQ30_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1966754.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(931..1305)
                     /gene="crcB"
                     /locus_tag="VYQ30_001684"
     CDS             complement(931..1305)
                     /gene="crcB"
                     /locus_tag="VYQ30_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1966755.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1302..1568)
                     /locus_tag="VYQ30_001685"
     CDS             complement(1302..1568)
                     /locus_tag="VYQ30_001685"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1966756.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1684..2127)
                     /locus_tag="VYQ30_001686"
     CDS             complement(1684..2127)
                     /locus_tag="VYQ30_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1966757.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2231..3166
                     /locus_tag="VYQ30_001687"
     CDS             2231..3166
                     /locus_tag="VYQ30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1966758.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3220..3504
                     /locus_tag="VYQ30_001688"
     CDS             3220..3504
                     /locus_tag="VYQ30_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1966759.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3561..3779)
                     /locus_tag="VYQ30_001689"
     CDS             complement(3561..3779)
                     /locus_tag="VYQ30_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966760.1"
                     /translation="MSISPRFETLEQAIAFKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3776..4530)
                     /locus_tag="VYQ30_001690"
                     /pseudo
     CDS             complement(3776..4530)
                     /locus_tag="VYQ30_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1398 a         1004 c          827 g         1301 t
ORIGIN      
        1 ctttgtcttt ctttacatta ctaaaaatgc cccctgcagg aatcgaacct gcaactactc
       61 cttaggaggg agttgttata tccattgaac taagggagct agataaaaac tctgctgaaa
      121 aagcagagtt ttttagtcga attaacgacg gatttctttg atacgagctg ccttaccttg
      181 aagagcacgc aagtagtaca atttcgcacg acgtacttta ccgtaacgaa caacttcgat
      241 tttttcaaca cgtggagtgt ggattgggaa gatacgctca acacctacac cgttagagat
      301 tttacgaact gtgtagtttt ctgagattcc agcaccttta cgtgcgataa caacaccttc
      361 aaaaatctgg atacgttcac ggttaccttc gacaactttc gcatgtacac gaacagtgtc
      421 accaggacgg aatgatggga tatctgtacg aagttgacct tcagtcaagc tttggattaa
      481 tggattcatt ttattctcct atctttgtca atcttgagga aacttcctca gcggataaac
      541 tgtatttttg tgcgtccatc acacacaaga tacagtctac caaattttca caaaaaagta
      601 aagaaattta tagcaaaatt cctaaaaaaa tcgcaaccaa accacctatg taagtcaaag
      661 tcaggtaaga ataaaagacc ttcttatcac ttagcagtct ttgaagttcg tcattcaagg
      721 tcgaaaaagt tgttaaaccg ccacaaaatc ctgttgctag aatagcatat acttccttag
      781 actctacatg attgtagaat actccaatta aaaaacaacc taaaagatta gctatgagcg
      841 ttccgagtgg caatttagaa gcttgattgt agcgggaaaa gaaataccgt acgagtgctc
      901 ccaagccaca ggcgattgca agatagacga ttaccatttc ttcctcccta gaaaataagc
      961 taagagtagg cctccaccga tgctcaaaag caaatacagt atcaaactaa gataacgccc
     1021 tgtatcaagc agcttcacag tatcaagcat taggctggaa aaagttgtta aacctccgca
     1081 aaaacccgtc cccagtgcta aaatcaagcc cttactagtc cccttataga ccagatagcc
     1141 cttgacaaga taaatcaagc agaaaattcc cagatagttg acaaagagag tgccccaagg
     1201 gaaatctgga ctggctggta accaagtgga aactaggtag cggacaagtc cgcccaacat
     1261 agcagctaga aaaatcccta gcggataaaa ttgttctttt ttcatttgat attttgatcc
     1321 tgataatcac gcgaacattt gagtatgtcc gaaaaagtcg cgacaatagt ctcctgatag
     1381 cgcttgtcct ctacacgact tctaaccttt tcaaaaataa ctgcttctct cttagtatct
     1441 aaaatcggtt tccctgaagc cttcttataa gcgacaactc cttcaactaa gtgcattcgt
     1501 tcttctaaca gtttgacaat ttggtcgtcg atttgatcga tttcttgacg aataatatct
     1561 aaatccatac agtctcctcc tttatttgaa ttattgtatc aaaaagccat caattaggct
     1621 agtaaaatcc caactctcga taagaaaaat gcactgattg attacatcag cgcactttta
     1681 tgcttatcct actttagcaa ccaattcttc tgcgaattgt tccaagcgtt caatatcttc
     1741 ttcctcggca gaaagatcaa ctttaacaca ctctgaacct ttttctgctc ctgttgacac
     1801 aaagacacgg tcaaagtcat caacagcctt acagaattca tcgtagaagg tatctcctga
     1861 gccgaccact ccgtagattt tgccattcaa gttgagatct gctaggtctt cgtagaagtc
     1921 catcatctca tctggcaatt caccatcacc ataagtatag gtcgcaacga tagcgatgtc
     1981 tgcttccaag aagtctgaag cgtcaacagt tgtacattca tcaacatcaa catccaagcc
     2041 caagtcacgt aatttgtctg ctacaatatc tgcaatttct tcggtattac cggtcatact
     2101 ggcaaataca atttttgcta atgccatatc gtcctcctca atttatcttt ctccattata
     2161 tcacatcttt ttcattttaa aaataaaaat tcttgtgcta caatgaaatg agaaagaatt
     2221 gaggttattt atggagattt gccaacaaat tttagaaaaa attaaagaat acgacaccat
     2281 tatcattcat cgtcatatga aaccagaccc tgatgccttg ggaagtcagg tgggattgaa
     2341 agccttgcta gaacatcatt tcccagaaaa aaccatcaaa gccgtcggtt ttgatgaacc
     2401 aactcttact tggatggctg agatggatct tgttgaagat agagcctacc aaggcgcact
     2461 tgtcatcgtc tgtgatacag ctaatactgc tcgtatcgat gataagcgct atagtcaagg
     2521 tgattttctc attaagattg accaccatcc aaatgatgat gtatacggtg acctgtcttg
     2581 ggtcgatact agttcaagta gcgctagcga gatgattacc ctatttgccc aaacaaccca
     2641 actagccttg gcagatcgcg atgctgagtt gctctttgca ggaattgtcg gtgatacagg
     2701 tcgcttcctc tacccttcta ccactgcacg gactcttcgc ctggctgctt atttgagaga
     2761 acataacttt gactttgcag ctctcactcg caaaatggac actatgagct acaaaattgc
     2821 taaactgcaa ggctacatct acgaccatct ggaagtggat gaaaatggtg ctgctcgcgt
     2881 tatcctgagt caagaagtct tgaaacaata caatgtaacc gatgctgaaa ctgcagccat
     2941 tgtcggtgca cctggacgca ttgacagagt gagtctctgg ggaatttttg tcgaacaggc
     3001 tgatggccac taccgagttc gcttacgcag taaaatccat cctatcaatg aaatcgccaa
     3061 ggagcatgat ggtggaggcc accctctagc aagtggtgct aattcctata gcctagaaga
     3121 aaatgaaatc atctaccaaa agttaaaaaa cttgcttaaa aactgataaa atacttgcca
     3181 aacttttcag aatctgatag actagtatgg taacaatcta tggctcgcaa agagaccatg
     3241 gcagaaagga aatattgcaa aatgaaaaaa gatatccatc cagaatatcg cccagttgtc
     3301 ttcatggaca caactactgg ttaccaattc cttagcggtt caacaaaacg ctctaacgaa
     3361 acagttgagt tcgaaggcga aacttaccca ttgatccgtg tggaaatttc atcagactca
     3421 cacccattct acactggacg tcaaaagttc actcaagcag atggacgcgt ggatcgtttc
     3481 aacaaaaaat acggtctcaa ataatgataa aagaacagtt tcgactgttc ttttttgttt
     3541 cttgaaatca actgctgttt tcatgttcca gactcatctg taggttcggt ttccatgcta
     3601 ctaggcagga aggaaatagc tgtttcaaca cgtccataat gagctgtact attgtcacga
     3661 accacacttt cattgatggt ccaagtggaa ttcattttct taaaagcttc tcggactttt
     3721 tccaaatctt tgaaggcaat ggcctgctct aaggtttcaa aacgaggact tatactcatc
     3781 tgctttcaca aagcattcta gtccatctcc gattaacgat ggactttatc acctccttct
     3841 ccagtccttg tatgacatct tgaagttgat tcatgatatc ttccaaagtt cgaaaggctt
     3901 tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggg ttcatctctg
     3961 gtgtgtatgg aggaataaag gtaaaaccaa tattagtcgg aatctttaag gtacttgatt
     4021 tatgccatat agcattgtcc ataacgaata aaagataatc atctggataa gcttgtgaaa
     4081 gctcttctaa aaaggcgttc atccactcag tattacatct accagctatt aagaaaaatg
     4141 attcgcctgt atgggcatca acagctccat aacaatagcg aaattctcgt atatagtgac
     4201 tatggacatg tggacctact cctattggag accaacaaga tcccagttta ctgattctac
     4261 cgaaaccagc ctcatcttgg tacatcaagc gaaccttatg aaaacgtcta ctatatttaa
     4321 aacgctttct tgtcttcttg aattgagatt ttatttttag acgcgacaat ggtttgagcg
     4381 tctgctttct taggatgttc tggacgtggc ataatatttc gccaaccatg gcgcttcaac
     4441 agttgataga aggcatcacg tgtgtaggaa cgatataagc ctgaaataag gtattgtaac
     4501 aaattctcct gcctctgtag ccttcaaatg
//
LOCUS       DAVMEF010000135         4489 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMEF010000135 DAVMEF010000000
VERSION     DAVMEF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.135"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(130..1116)
                     /locus_tag="VYQ30_001691"
     CDS             complement(130..1116)
                     /locus_tag="VYQ30_001691"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1966761.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1310..3778
                     /gene="gyrA"
                     /locus_tag="VYQ30_001692"
     CDS             1310..3778
                     /gene="gyrA"
                     /locus_tag="VYQ30_001692"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1966762.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPIVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3778..>4489
                     /gene="srtA"
                     /locus_tag="VYQ30_001693"
     CDS             3778..>4489
                     /gene="srtA"
                     /locus_tag="VYQ30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1966763.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILT"
BASE COUNT         1366 a          792 c         1015 g         1316 t
ORIGIN      
        1 aaatcaatta ggaatttaaa gcattttata acaacgtttt agagtaatgg ggggctattt
       61 caacttcaac ctactataat acagaaaaaa acaactccct gataattcaa ggagttgtct
      121 atagttaaat tagtttttag aagcttcttg gaattctggg tttttccatg cttcgtcaat
      181 gatagcttgc aattctttag cagatgcttg cattttttga gtttctgcgt cgttcaatgg
      241 gatatttact ggacgaacaa taccatgtgc accaacaaca gctggttgac cgataaagac
      301 gttctcaact ccgtattgac cttcttggaa tactgaaagt ggaagtactg cattttcatc
      361 gtcaaggatt gctttagtga tacgagcaag ggctactgcg ataccgtagt atgttgcacc
      421 ttttttgttg atgattgtgt aggctgcatc acgaacacct tcgaacaatt caatcaattc
      481 agcttcttga acattttgag tatctttaag gaattcttca aggtttacac cagcgatgtt
      541 agcgtgtgac caaacagcga actcagagtc accgtgttca cccatgatgt aggcgtgaac
      601 tgaacgagca tctacgtcca atttttcagc aagtgcttga cggaaacgag ctgagtcaag
      661 tgaagtacct gaaccgataa cgcgttcttt agggaaacca gagaatttcc aagttgagta
      721 agtcaaaacg tcaactgggt tagcagcaac aaggaagata cctttgaaac cagattcaac
      781 aacttgagtt acgattgatt tgttgatagc aaggttttta cctacaaggt caagacgagt
      841 ttcacctggt ttttgaggtg cacctgcagt gatcacaaca aggtcagcgt ctgcacagtc
      901 agagtattga gctgcataga tttttttagg tgaagtgaag gcaagggcgt gactaaggtc
      961 aagcgcatca ccaacagctt tttcatgcaa ttgtggaatt tcgataattc caagctcttg
     1021 tgcaattcct tggttaacaa gtgcaaaagc gtaagatgaa cctacagcac catcaccgac
     1081 aaggataact tttttgtgtt gtttagttga agtcattgtt ttaaacatct ccttaatttt
     1141 attaggggat tttccctaga caacttcatt ctatcacttt taaaaaactt tgtcacgaat
     1201 atgccttata gttctcgatg taaacgtttt agtggtttag aggctgaaat agatgggaat
     1261 ttatggtata atgttgttac ttactaattg tgaaatgagg catttattaa tgcaggataa
     1321 aaatttagtg aatgtcaatc tgacaaagga gatgaaggca agttttatcg actacgccat
     1381 gagtgttatc gtagcgcgag ctcttcctga tgttcgagat ggcttaaaac ctgttcaccg
     1441 tcgcattctc tacggaatga atgaattggg tgtgacccca gacaaacccc ataaaaaatc
     1501 tgctcgtatt acaggggatg tcatgggtaa atatcaccca cacggggatt cctctattta
     1561 tgaagccatg gtccgtatgg ctcaatggtg gagctaccgt tacatgcttg tagatggtca
     1621 tgggaatttt ggttccatgg atggagatag tgctgccgct caacgttata ccgaggcacg
     1681 tatgagcaag attgctctgg aaatgcttcg tgatatcaac aaaaatacag ttgatttcgt
     1741 tgataactat gatgccaatg aacgggaacc cttggtcttg ccagcgcgtt ttccaaacct
     1801 tttggttaat ggagcaactg gtatcgcggt tgggatggca accaatattc cacctcataa
     1861 tctgggtgaa accattgatg cagtgaagtt ggtcatggat aatcctgaag tgactaccaa
     1921 ggacttgatg gaagtcttgc ctggaccaga ttttccaact ggtgctcttg tcatggggaa
     1981 atcaggtatc cataaggctt atgaaacagg taaaggttcg attgtcctac gttctcgtac
     2041 agagattgaa acgactaaga ctggtcgtga gcgcatcgtt gtaacagaat ttccttacat
     2101 ggtcaataaa accaaggtgc atgagcatat tgttcgcttg gttcaggaaa aacgcattga
     2161 gggtatcaca gcagtacgtg atgagtcaaa ccgtgaaggt gttcgatttg ttattgaagt
     2221 caagcgcgac gcctcagcca atgttattct caataacctc ttcaaaatga cccaaatgca
     2281 aaccaatttt ggtttcaata tgctcgctat ccaaaatggt ataccgaaaa ttttgtctct
     2341 tcgtcagatt ttggatgctt atatcgagca ccaaaaagaa gtggttgttc gtcgtacacg
     2401 ttttgataag gaaaaagcgg aagcgcgcgc tcatatctta gaaggtctct tgattgcgct
     2461 agaccatatc gacgaagtga ttcgtatcat ccgtgctagt gaaacggatg cggaagctca
     2521 agctgagttg atgagcaagt ttaagctttc tgaacgtcaa agtcaagcta tccttgatat
     2581 gcgtcttcgt cgtttgacag gtttggaacg cgataagatt caatctgagt atgatgacct
     2641 cttggctctg attgcggatt tagcagatat tcttgctaag cctgaacgtg tttctcaaat
     2701 tatcaaagac gaattggatg aagttaaacg taaattttct gataaacgcc gtacagagtt
     2761 gatggttgga cagatcttga gtctcgagga tgaggacttg attgaagaat cggatgtctt
     2821 gattaccctt tctaacagag gctacattaa gcgtttggat caggacgagt tcactgctca
     2881 aaaacgtggg ggtcgtggtg tccaaggaac gggagtgaaa gatgatgact ttgttcgtga
     2941 gttagtgtca actagcacac atgatcatct gctcttcttc acaaacaagg gacgtgtcta
     3001 tcgtcttaaa ggttatgaaa ttcctgagta tggtcggact gccaaagggc taccaatagt
     3061 caatctcttg aaattggatg aagacgaaag tattcagacg gttatcaatg ttgagtctga
     3121 tcgcagtgat gatgcttatc tcttctttac aacccgtcac ggtattgtga agagaactag
     3181 tgttaaggag tttgccaata ttcgtcaaaa tggtctcaaa gcgctgaatt taaaggatga
     3241 agatgagtta atcaatgtct tgttggcaga aggagatatg gatattatca ttggtaccaa
     3301 gtttggttat gcagttcgct ttaatcaatc agccgttcgt ggtatgagtc gtatcgccac
     3361 tggtgtgaaa ggtgttaacc ttcgtgaagg agacacagtt gttggtgcca gcttgattac
     3421 tgatcaagat gaggttctta ttatcacaga aaaaggatat ggtaagcgta cagtcgctac
     3481 tgaataccca acaaaaggtc gtggtggtaa gggaatgcag acagctaaaa ttaccgaaaa
     3541 aaatggcttg ctggccggtc ttatgactgt tcaaggggat gaggatttga tgattatcac
     3601 tgatacaggt gtcatgattc gaaccaatct tgccaatatt tcacaaacag gacgtgcaac
     3661 tatgggagtt aaagtaatgc gcctggatca agatgctcag atagtgactt tcacaacggt
     3721 tgcggtggca gaaaaagaag aagttgggac agaaaacgaa acagaaggtg aagcataatg
     3781 tctcaaaaaa ataataaaaa gaaaaacaag cgaaaaaatc tgctgacaaa tatcctagca
     3841 ggatttctga tattactgtc actggctttg atttttaata ctcaaattcg aaatattttc
     3901 atagtctgga ataccaataa gtatcaagtt agccaggtat caaaagaaaa attagaagaa
     3961 aatcaggata cagaaggcaa ttttgacttt gattctgtca aagctatctc ttcggaagct
     4021 gttctaactt ctcaatggga tgctcaaaaa ttaccagtta ttgggggaat tgcaattcct
     4081 gaattggaaa tgaatttgcc gatttttaaa ggacttgata atgttaatct cttctacgga
     4141 gctggtacaa tgaaacgcga gcaagtaatg ggagaaggaa attatagtct agctagtcac
     4201 catatctttg gtgttgataa tgctaataaa atgttatttt ctcctttaga taatgctaaa
     4261 aatggcatga agatttatct aactgataaa aataaagttt atacttatga aatacgtgaa
     4321 gtcaaacgtg tgacaccaga tcgtgttgat gaagttgatg atagagatgg ggtcaatgaa
     4381 atcacattag taacctgtga agaccttgct gctacagaac gtattattgt caaaggtgat
     4441 ttgaaagaaa caaaagatta ttcacaaaca tctgatgaaa tcctaacag
//
LOCUS       DAVMEF010000136         4479 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMEF010000136 DAVMEF010000000
VERSION     DAVMEF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.136"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..1874
                     /locus_tag="VYQ30_001694"
     CDS             24..1874
                     /locus_tag="VYQ30_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1966764.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2135..2767)
                     /locus_tag="VYQ30_001695"
     CDS             complement(2135..2767)
                     /locus_tag="VYQ30_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1966765.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2789..3661)
                     /gene="sdaAA"
                     /locus_tag="VYQ30_001696"
     CDS             complement(2789..3661)
                     /gene="sdaAA"
                     /locus_tag="VYQ30_001696"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1966766.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3670..4341)
                     /gene="sdaAB"
                     /locus_tag="VYQ30_001697"
     CDS             complement(3670..4341)
                     /gene="sdaAB"
                     /locus_tag="VYQ30_001697"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1966767.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
BASE COUNT         1229 a          899 c         1004 g         1347 t
ORIGIN      
        1 cttttaagga ggagtagatt tctatgaata aaaaactaac agattatgtg attgatctgg
       61 tggaaatttt aaataaacaa caaaagcagg ttttctgggg aatatttgat attttcagta
      121 tggtggtttc catcattgta tcttatattt tattttatgg gctgattaat ccagcacctg
      181 ttgactacat tatctatacg agtttggcct tcctgttcta tcaattgatg attggttttt
      241 gggggttgaa cgcgagcatt agtcgttaca gcaagattac ggatttcatg aaaatctttt
      301 ttggtgtgac tgctagcagt gtcttgtcat atagtatctg ttatgccttc ttgccactct
      361 tctccatccg tttcatcatt ctctttatct tgttgagtac cttcttgatt ttattgccac
      421 ggattacttg gcagttaatc tactccagac gcaaaaaagg tagtggtgat ggagaacacc
      481 gtcgaacctt cttgattggt gccggtgatg gtggggctct ttttatggat agttaccaac
      541 atccaaccag tgaattagaa ctggtcggta ttttggataa ggattctaag aaaaagggtc
      601 aaaaacttgg tggtattcct gttttgggct cttatgacaa tctgcctgaa ttagccaaac
      661 gccatcaaat cgagcgtgtc atcgttgcga ttccgtcgct ggatccgtca gaatatgagc
      721 gtatcttgca gatgtgtaat aagctgggtg tcaaatgtta caagatgcct aaggttgaaa
      781 ctgttgttca gggccttcac caagcaggta ctggcttcca aaaaattgat attacggacc
      841 ttttgggtcg tcaggaaatc cgtcttgacg aatcgcgtct gggtgcagaa ctgacaggta
      901 agaccatctt agtcacagga gctggaggtt caatcggttc tgaaatctgt cgtcaagtta
      961 gtcgcttcaa tcctgaacgc attgtcttgc tcggtcatgg ggaaaactca atctaccttg
     1021 tttatcatga attgattcgt aagttccaag ggattgatta tgtacctgtg attgcggaca
     1081 ttcaagacta tgatcgtttg ttgcaagtct ttgagcagta caaacctgct attgtttatc
     1141 atgcggcagc ccacaagcat gttcctatga tggagcgcaa tccaaaagaa gccttcaaaa
     1201 acaatatccg tggaacttac aatgttgcta aggctgttga tgaagctaaa gtgtctaaga
     1261 tggttatgat ttcgacagat aaggcagtta atccaccaaa tgttatggga gcaaccaagc
     1321 gcgtggcgga gttgattgtc actggcttta accaacgtag ccaatcaacc tactgtgcag
     1381 ttcgttttgg gaatgttctt ggtagccgtg gtagtgtcat tccagtcttt gaacgtcaga
     1441 ttgctgaagg tgggcctgta acggtgacag atttccgcat gacccgttac tttatgacca
     1501 ttccagaagc tagccgtctg gttatccatg ctggtgctta tgccaaagat ggggaagtct
     1561 ttatccttga tatgggtaaa ccagtcaaga tttatgactt ggccaagaag atggtgcttc
     1621 taagtggcca cactgaaagt gaaattccaa tcgttgaagt tggaatccgc ccaggtgaaa
     1681 aactctacga agaactcttg gtatcaaccg aactcgttga caatcaagtt atggataaga
     1741 ttttcgttgg taaggttaat gtcatgcctt tagaatccat caatcaaaag attggagagt
     1801 tccgcactct cagtggagat gagttgaagc aagctattat cgcctttgct aatcaaacaa
     1861 cccacattga ataaaaaaga aaaacgcata ttatcaagtt aaataacctt ggtaatatgc
     1921 gttttattat gtagagactt atactcttcg aaaatctctt caaatcacgt caacgtcgcc
     1981 ttgccgtatg tatatgttac tgacttcgtc agttctatct gcaacctcaa aacagtgttt
     2041 tgagcagccc gcagctagtt tcctagtttg ctctttgatt ttcattgagt attacttcat
     2101 tttcttctga aatggaattg ttacccagtc tatgctattg aaaatacgcc aaaacttcta
     2161 agggtttgtg agcgatataa tcaggttgat agtttagtag atctgcttgc tctccaaatc
     2221 cccaagtgac ggccaatttc tgaatacctg tttctcgagc tcccagcata tcaaacttgg
     2281 tatctccgat gatgatggct tgttctggtg ctagttgatg tgtctgcaag gcttggtgaa
     2341 tgacatctgc cttatggggt gcttcagggc tagaaccata aatgccatca aagaaatgat
     2401 ggatttccaa gttttttgcc atgtcttgag cagtagatgt attctttgtc gtggtgatgt
     2461 agagtggata actgctcgat aactcctcaa gcaagtctat aatctgagga aagagttgag
     2521 cttcatagat gccttttgcc ttatagtaag aacgatatat ctgcacggct tcagaaattt
     2581 ggtctttgga caggcaggtc gcaaaactac tttcgagagg tggtcccata aaaccacgaa
     2641 tagttttggc atcagggcta ggcaccccca gctctttaaa ggtataggta aaggcattgt
     2701 gaatcccgat agaactatca acgagggttc catccaaatc gaaaaaaatc gctgtgatag
     2761 aggtcatggt ttctcctatt tgataagctt attctccgaa aatttctttt tggaggcgac
     2821 gaccagtagg ggtggtagcg agtccacctt cagctgtttc acgaaaggca gttggcatgc
     2881 ttgctcctac ttggtacatg gcatcgatca cttcatccac agggatttta gattcgatac
     2941 ctgccaaggc catgtctgct gcgatgaaag caaagctagc tcccatggca ttacgtttga
     3001 cacagggaac ttcgaccaaa cctgcaacag ggtcacagat gaggcctagc atatttttaa
     3061 tgacaaaggc aatagcttga ctggcctgat aaggtgttcc acctgcagcc agagtcaagg
     3121 cggcagcact catagcagag gctgaaccaa cttcagcttg acacccaccc tcagcacctg
     3181 agatggaggc attgtttgcg atgactagtc caaaggcacc agcagcaaag aggaaatcca
     3241 attgttgctc gtggctgagg tctaattttt caatagcagc agtgagaacg gatggcagac
     3301 agccagcact tccagcggtt ggagtggcac agaccaagcc cattttggca ttgtgttcat
     3361 tgactgcgat ggcatttcgg gcagccgaga gaatcgtata atctgacaga gtttttccgt
     3421 tttcgatgta gtgatccaat ttggcagcat ctccacctgt caggccacta cgagatttat
     3481 tttcattgag gccaagttgg acagaggctt tcataacttc cagattgcgt tccatgagaa
     3541 ggaagacttc ttcacgttcg cgaccggtca attcaaactc tgttgtaatc atgagttctg
     3601 cgacatttcc ttgaaagtcc agatctgctt gctcgaccaa ttctttgata gaataaaaca
     3661 tgcttcctcc tatttaaaga aattgacatt gtggagatga gggatttttc gaatttcttc
     3721 gatagcctca tcacagttgc gactgtcaac ttcgataatc ataatggctt tttcaccagc
     3781 tttttcacga gtgacattca tctgggcgat attgatacca tagcgggaaa gcgcctctgt
     3841 aacaagggca atcatacctg gaatatcttg atgaacgatg atgatagtcg gtgtattcat
     3901 attgagagag acggcaaaac cattgagttc ggttacctga atatttcctc caccgataga
     3961 aataccagtc acgctgatgg tcttgtgggc atttttaaca gtaattttag tggtgttagg
     4021 gtgaggggca ttgctgtctt tctgaatggt ccagacaatc ttgataccac gcttgtgggc
     4081 aatttccaga ctatttggaa tttcaggatc atctgtatcc attcctaaaa tacctgcaac
     4141 aagggctagg tctgttccgt gaccacgata ggtcttggca aatgagttaa aaagttggaa
     4201 ttcaacttct gtcggagtat catcaaaaat ggaagagaca atcttcccaa tacgaacagc
     4261 accagcggta tggctactag atgggccaat cataactggt ccgatgatat caaagacaga
     4321 ttgaaaacga agtgatttca tcagtttccc cttataaaaa ttcttatctc tattatatca
     4381 aagaatgagg ggcttggctt taattgtgga tgaaaacctt tctaatacct caaatagcat
     4441 aaaaatagta tcttttatga caaaaacacc ttatttagg
//
LOCUS       DAVMEF010000137         4425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMEF010000137 DAVMEF010000000
VERSION     DAVMEF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.137"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            181..1947
                     /locus_tag="VYQ30_001698"
     CDS             181..1947
                     /locus_tag="VYQ30_001698"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1966768.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            2093..3244
                     /locus_tag="VYQ30_001699"
     CDS             2093..3244
                     /locus_tag="VYQ30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1966769.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            3389..4273
                     /locus_tag="VYQ30_001700"
     CDS             3389..4273
                     /locus_tag="VYQ30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1966770.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         1345 a          805 c          997 g         1278 t
ORIGIN      
        1 tttagaaagc aataatctat ccatagactt aaaaggactg aagggaaaaa aggttagaat
       61 ttctttgaga aacgcaggaa ttcctttaag tttagcagag gtagaggttt atacttataa
      121 gtaattttaa aaattatcat cggtagtatg aaagaaacag aaaaataaga ggaaaatagt
      181 atgattcaac atccacgtat tggtattcgt ccgactattg atggtcgtcg tcaaggtgta
      241 cgcgaatcac ttgaagtaca aacaatgaac atggctaaaa gtgtggcaga tttgatttca
      301 agcacattga aatatccaga tggggaacct gtggaatgtg tgatttctcc atctaccatt
      361 ggtcgtgttc cagaggctgc agcttcccat gagttgttta aaaaatcaaa tgtttgcgca
      421 acaattacag ttacaccatg ctggtgttat ggtagtgaaa ctatggatat gtctccagat
      481 attcctcatg ctatttgggg atttaatggg acagaacgcc caggagctgt ctatcttgca
      541 gctgtactag cttcacatgc tcaaaaaggg attccagcct ttgggattta tggaagagat
      601 gttcaggaag ctagtgacac agatattcca gaagatgtca aagaaaaact tttacgctat
      661 gcgcgtgcag ctcttgcaac tggcttgatg agagacactg cttacctatc aatgggtagt
      721 gtttcgatgg ggattggtgg ttctattgta aatccggatt tcttccaaga atacctagga
      781 atgcgaaatg aatcggtaga tatgacggag ttcacgcgcc gtatggaccg tagtatttac
      841 gaccctgaag agttcgaacg tgcgctcaaa tgggtgaaag aaaacgtaaa agaaggattc
      901 gaccataacc gtgaagacct tgttttaagc cgtgaagaaa aagatagaca atgggaattt
      961 gttattaaga tgttcatgat tggacgtgac ttaatggttg gtaacccaag acttgctgaa
     1021 cttggttttg aggaagaagc agttggtcac catgctttag tagctggttt ccaaggtcaa
     1081 cgtcagtgga cagaccattt tccaaatgga gattttatgg aaactttcct caatactcag
     1141 tttgactgga atggtattcg aaaaccattt gtatttgcga cagagaatga ttcactaaat
     1201 ggtgtgtcta tgctctttaa ttatctacta acaaatactc cacaaatctt tgctgatgtg
     1261 cgtacttatt ggagtccaga ggctgttgaa cgtgtaacag gatatacttt agagggtcgt
     1321 gctgcagctg gcttcttaca tctaatcaac tctggttctt gtacattgga tggtacaggt
     1381 caagctactc gagatggcaa acctgttatg aaaccattct gggagttgga tgaaagtgaa
     1441 gtacaggcta tgcttgaaaa tacagacttc ccaccagcaa accgcgagta cttccgtgga
     1501 ggaggattct caactcgttt cttgacgaag ggggatatgc cagtaacaat ggtacgtctc
     1561 aatcttctaa aaggggttgg tccagtgcta caaattgcag aaggttacac acttgaactt
     1621 cctgaagatg ttcaccatac tttagataat cgtacagatt caggatggcc aactacttgg
     1681 tttgctccac gtttgacagg aaaaggtgct ttcaagtctg tctatgacgt catgaataat
     1741 tggggagcta atcacggagc cataacatat ggacacattg gagcagactt gattaccttg
     1801 gcttctatgt tgagaattcc tgtcaatatg cataatgtac ctgaggaaga tatctttaga
     1861 cctaaaaatt ggtccttatt tggaacagaa gatctagaat cagcagacta tcgtgcatgt
     1921 cagttgttgg gaccactaca taaataaaac ttgtttatat aggaggtgaa cttacgtccc
     1981 tcctatcctt ttaaaaagat ttgttaaaca attcacaaat aattgaaaac gaatacaaaa
     2041 agtaatataa tgatgttaaa tagatagcgc ggaggcgcag gaggaaaatt atatggctat
     2101 attttatgtt ccggcagtca accttattgg aaaaggtgtt gtaaatgaag tgggtcctta
     2161 tatcaaggaa cttggctata aaaaggcact tttggtgaca gataagtaca tcgaaggcag
     2221 tgatatttta cctaagactt taaaaccact ggatacagaa ggaatcgaat atgtcatctt
     2281 tagcgatgta gagccaaacc ctacttgtaa aaatgtcaca gatggggtag ctgctttgca
     2341 agaacatggc tgtgacttta tcatcagtct tggcgggggt tctccacagg atgcagctag
     2401 ttgtatttct atcatggcta caaatggtgg aaaaccacag gattatgaag ggcttcataa
     2461 gtctgctaaa aaaggtttgc cagttgtggc tatcaataca acggcaggta catcagcaga
     2521 aattaccatt aactatgtga ttactgatga agaacgcaag gttaagatgg taatggttga
     2581 caagaatagc cttgccttga tttctgtcaa tgaccctgaa ctcatgcttt ccaaacctaa
     2641 aggcctgact gctgctactg gtatggatgc tctgactcat gctgttgaag ctttggtaac
     2701 acctggtgct tatgatgtaa ccaagaaact gtctattggt gctattgagc ttatcaagga
     2761 atatcttcct cgtgctgtag aaaatggaca tgatattgaa gcgcgtgaag gtatggtcaa
     2821 tgccatcttc cttggtggta tgagctttaa taatgctggt cttggctatg ttcactcaat
     2881 ggctcaccaa ctcggtgcag tatataattt gccacatggc gtgtgctgtg ccatgttgct
     2941 accagttata gaacgtgaaa atgctaaacg tgtaccagaa gctttccgca atgttgccaa
     3001 agccttggga cttcatgtag aaggtaaatc agatcaagaa tgtgccgatt atgcgattgc
     3061 tgagattgag aagctttctg agacagtagg tattcctaag aaactgactg aacttggtat
     3121 tgaagaaaaa gatttcgact ttgaatacct ttctaagaat gccttgattg atgcctgcgc
     3181 accaggaaat ccatttatgc caaccttaga agaaacgatt gccttttata aagagttatt
     3241 ttagcaagag attggacgac aataggacaa ttcacctcta attattgaca tttcaggaaa
     3301 aatcgcagta aaaacggcac agccttcttt aaatgtgcta taatactaga aaaatacttg
     3361 tggaggttcc attatggcaa tatttttcat gatttttctg attgtttgtg tgctcctatt
     3421 ggtgatagtc acactgagta cagtttatgt ggttcgtcag cagtcggtgg cgattattga
     3481 acgctttggg aaataccaaa aggttgctaa tagcggtatt catattcgct tgccttttgg
     3541 gattgactcg attgcagcac ggattcagtt gcgcttgttg caaagtgata ttgtggttga
     3601 gactaagacc aaggacaatg tgttcgttat gatgaatgta gcgactcagt accgtgtcaa
     3661 cgagcagagc gtgacagatg cttactataa actcatacgt ccagaatctc agattaaatc
     3721 ttatatcgaa gatgctcttc gctcttctgt tccaaaatta accttggatg aattgtttga
     3781 gaaaaaagat gagattgccc ttgaggttca acaccaagta gcagaagaaa tgaccactta
     3841 cggctacatt atcgtgaaaa ccttgattac caaggtcgaa ccagatgcag aagttaagca
     3901 atctatgaat gaaatcaatg cggcgcaacg taagcgggtc gcagcacaag aattggcgga
     3961 agctgacaag attaaaattg tcactgcagc tgaagccgaa gcagaaaaag accgccttca
     4021 tggtgtgggg attgcccaac aacgtaaggc gattgtggat ggattggcag agtctatcac
     4081 cgaactcaag gaagccaatg ttggcatgac agaagaacaa atcatgtcta tcctcttgac
     4141 caaccagtat ttggatacct tgaatacctt tgcctctaaa ggaaatcaaa ccatcttttt
     4201 accaaatact ccaaatggtg tggatgatat ccgtacacaa atcttgtcag cccttcgcgc
     4261 tgagaagaaa taatagacta atactcttcg aaaatctctt caaactacgt cagcgtcgtc
     4321 ttgccgtata gatgttactg acttcgtcag ttctatctgc aacctcaaaa cagtgttttg
     4381 agcaacctgt agctagtttc ctagtttgct ctttgatttt cattg
//
LOCUS       DAVMEF010000138         4405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMEF010000138 DAVMEF010000000
VERSION     DAVMEF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.138"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..347)
                     /locus_tag="VYQ30_001701"
     CDS             complement(<1..347)
                     /locus_tag="VYQ30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966771.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMING"
     gene            complement(622..2361)
                     /locus_tag="VYQ30_001702"
                     /pseudo
     CDS             complement(622..2361)
                     /locus_tag="VYQ30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(2524..3408)
                     /locus_tag="VYQ30_001703"
     CDS             complement(2524..3408)
                     /locus_tag="VYQ30_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1966772.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(3520..4374)
                     /locus_tag="VYQ30_001704"
     CDS             complement(3520..4374)
                     /locus_tag="VYQ30_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1966773.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEATYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
BASE COUNT         1319 a          942 c          691 g         1453 t
ORIGIN      
        1 tagccattaa tcatctcagt cagagttttc cctttactac cattctttat agtagtattt
       61 actcttttta tactgctttc aaaaggctca tatgttttat acttaattac ttcgtcttta
      121 aaattaaata aaggtgtatc cggcttagca ttccataaaa taatcaacca cattataaca
      181 ttataaagct gctttgcagt ttcattccac ttttcaggtt gtaaatttcc actccctgta
      241 ttgtcatatt cttgtctatt atttacatga gaatcaccta caagcggatt aaaaagaggt
      301 agccctttgt gattaattag ttctaaaaaa tcccttgccg cacccatgca acactgaata
      361 acttccttct tgccatcaat tttactctca gcgataaata cataatattc atcttcaata
      421 gttgctcctg catcactatg tttttgttga tttgctaaca gcttaatatg tgctttaggc
      481 taaacttcaa aatctctaac aattctttgt cttgtttcat gtcctctaca attcatttaa
      541 gttctccttt acaagtttag taactagcct tattatacta tataagatat gaaatgtacc
      601 ataatcttca tcaactagtt tttagaaaat cacaaatcca tctggagaga tgacaagctg
      661 attctccaat tcttggcaat tgcttgctag tactactgct tctttctcta aaagataatc
      721 ttctgttgat tggttgaata ttgctttgag gatccgtcct tcgtatttcc attccagagc
      781 tactagatca gatttgattt cttgaaggct atacttgcca tgcgaaatga ttactgacgc
      841 gtatttccga atcttaatca gcctcttcat aaagttcagc atatcattgt cacttgatac
      901 acgttcccaa ggcatacaac gacgacaatc tggatctggt cctccagtca aggctagctc
      961 ggttccgtaa taaatgcacg gtgttccttt ttgtaaaaag agaaaggcta aggctgattt
     1021 aaccagttga acatcttcat tggccgtcca caggattcgc tctgtatcat gtgaatccaa
     1081 gagattaaac atgacctctg aaatctgctg cttgtaatac atagactctc cattgatttc
     1141 atcgatgaac tggtctgtat tcttaattcc tcgtaagaaa tagtccttga tactatcaga
     1201 taaaggataa ttcatgacgg catggaactc atctccattt agccaaggct gagatgtatg
     1261 ccagacttct cctaggatat aaagatcagg atttttagct aaaactgcct tacgaaaatc
     1321 cttccagaac tgatggtcaa tctcattagc cacatccaaa cgccaagcat cgatattaaa
     1381 ctcttcaatc caataagtcg caacctttaa aagataattc ttgacctctg gattggctgt
     1441 atttagctta ggcatatagt cctcgaaacc aaaaacatga tagggtaagt ctctcttatt
     1501 aactagcttt tcagttgtca ctgggaattg ttgaatatgg aaccaatcct tataagcaga
     1561 ctgttcacca tttttgacga cattttttca ttgaagagat tgcgaaccaa tatgattaaa
     1621 taccgcatcc agcatgactt tcatgccacg atgatgcgct tgatccacca gttcccgaaa
     1681 ggtctccttg tctccaaaat gacggtcaat ttcaaagtaa tctgtcgtat tgtacttgtg
     1741 attgcttgta gattcaaaga tgggacaaag atatagtcca gtaataccca agtcttgcaa
     1801 gtaatccata tgatcaataa tcccctgtaa atcaccacca aagaaatcat cactcttagg
     1861 tgtgacagat gaatcccagt ctaaagtccc ttctgggttt aatagagcat tgccattggc
     1921 aaatctttca ggaaatatct gataccatac cgtatttgaa accccgtcag gaaccttgca
     1981 ggcatcaatc tcatgaagat aaggcaactt aaatccattc ccaatttcat gaagattttc
     2041 tagagaattt tccacacacc ctttatcgcc atacaaaata ttttgacctt ctgtatctct
     2101 gagctcaaag agatactgga tacgtgcaaa gtcaactgac acttcaacct gccaatggtc
     2161 aaataaggta ccagaagtta tcttgaccat ttcttttgta tcctgataaa actcctccat
     2221 aaagataaaa gggtccccat agtgcaagtt gatgctttca atgtcccctt tcttagttcg
     2281 aattcgaata tggagtttct tatccttata aagataggca tactccgact caggcccatg
     2341 gtaaatagca cttaattcca tcaatttgtc tcctaaagta ttcattcaaa ttttacgcaa
     2401 acgttttcaa actcaataaa tccagtatac tatttttgta aactatttgc aaggaatgtc
     2461 cgttgttaaa ggaactatct tttaaagtat acaaaaagcc ttaaaaaagg ctttctctat
     2521 atactaatcc tctttcggtt ttgaaaatac ttctactcgc tgagctagga ctttgatttc
     2581 tacaggtagg ttatctgatt tatctccgtc aacatctgac tccaattcac tatctgaaga
     2641 gattttaata ttacgcgctt tgatatactc gatattatca tttgcgataa catccccttt
     2701 taataaatca ggaatgacgg ataatttgga gaatatagag gcatctttca gaatcaaaat
     2761 gttggcatag ccgtccttgt tttcttcaaa gattttctta tcagcgaagt aatttgtcaa
     2821 aaggaccaaa acatggctag cttcaccaac ataatttcca ttttctgtct caaccttaat
     2881 gttaaagacc tgatctgtca tgacagactt catggtattt acagcatagg ttaaaatacc
     2941 gaattttgtt ttgtcctcta tttcaacatt gtgaatcgcc tcaggcagag aaccgatact
     3001 aaagatataa ccaaaatagt tgtcatttgc tttaccgata tcaatcttat tggttaaatc
     3061 aaaatccagt tcgtcaattg cgccatcgat gtcttgattg atttccaaaa gttttgtaat
     3121 gaggttaccc gtaccgcctg ggataatccc taacttagga atgtagtctc tctcatcaat
     3181 acctgaaatg acttcattga cagttccatc tccaccaaac acaaccactg catcatactg
     3241 ctcacgagaa gcttcttcag caaaatgtgt tgcatccagc gctttttcgg taattttggt
     3301 ttcaacatat tcaaagtatt cttttgcttt attctccagc ttttctttgt aatccaaagc
     3361 cttctcgcca ccagaagtag ggttgataat taccattgct tttttcattg attttatcct
     3421 taattttaaa cagaaatgtt tacatttcgt cgtatgcaag taaatgtaat cctattatac
     3481 aatgaaaata cagaaaagag aaatctgacg tactggagat taatacgctt ttattctatt
     3541 ttcccatcgc ctaactacat cctttaaggg ttcatccaag taagaatagg ccttatcctt
     3601 gatccaatca ggaataccgt aagttgcctc tgctatgcta caagtgattg ctgcgagagt
     3661 atcactgtcg ccaccaagtg agatggcatt tcttatcgca tcttcgaagt ctctactttc
     3721 aagaaaggcg ataatggctt gagggacagt ttcctgacat gtttcgttaa aacgatagtt
     3781 aggacggatt tcatctaaag tttgagatag attgtaatcg tattcttttt ctatataatc
     3841 cttaattcgt cttttacact ctgtaggatt gtcgttaatt gattgctcgt actctctaca
     3901 ataaccacca aagtaaaaac gacatagaaa gatagcatca gctgtagcca tagcgccttt
     3961 gacaccttct ggatgattat gagttacctc tgcagaaaga ctcgtaagtc ctctagatga
     4021 tggccacata ccagttttcg cataaaaacc acagtccatg atccaagcac atggagaaat
     4081 acgcatagct gatccattcc caaagctatt ataaggctca cggttatcgc tgtttagcca
     4141 tgcattaaac cgagcaccgt aatcagcatt cggatacatt ctgccatatt tcttcatcgc
     4201 gtcaatgaaa tcgtcttttt gtccaccatt cataattgct tctgcaacag cacaggtcat
     4261 aaccgtgtca tctgtaaaaa agcagtcctt ccgaaataaa ggaaagtcct ttgttttgat
     4321 attgttccat tcgtaaacag aaccgacaat atctccaata attgctccaa gcatcagatt
     4381 cctccttgtt cattttgatg ctttt
//
LOCUS       DAVMEF010000139         4385 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMEF010000139 DAVMEF010000000
VERSION     DAVMEF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.139"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            307..606
                     /locus_tag="VYQ30_001705"
     CDS             307..606
                     /locus_tag="VYQ30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1966774.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVIDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(947..1921)
                     /gene="pta"
                     /locus_tag="VYQ30_001706"
     CDS             complement(947..1921)
                     /gene="pta"
                     /locus_tag="VYQ30_001706"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1966775.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1965..2861)
                     /locus_tag="VYQ30_001707"
     CDS             complement(1965..2861)
                     /locus_tag="VYQ30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1966776.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     DIRLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLDDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2858..3676)
                     /locus_tag="VYQ30_001708"
     CDS             complement(2858..3676)
                     /locus_tag="VYQ30_001708"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1966777.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3660..4331)
                     /locus_tag="VYQ30_001709"
     CDS             complement(3660..4331)
                     /locus_tag="VYQ30_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1966778.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
BASE COUNT         1353 a          953 c          798 g         1281 t
ORIGIN      
        1 actatttatc caaaaataga tagatgtaac atgtttgcaa acaaagcata cgaaccttta
       61 gtaaaatcat ttccatgaaa ctagaataga gccctcttag caaaaatcat tattttaatt
      121 tatttctaat cactccttga cataaataac tctcaccaat aaaagactat gtcttaaaaa
      181 aatggtataa taaaatcaat acttgggctt gatggctatg ctactaataa caattaggag
      241 agaaaatcag gcacttgtta acaacaagga ttatcccctt gagatgaaag gaactttaga
      301 aatcttatga tgaacatgca aaacatgatg cgtcaagcac aaaaacttca aaaacaaatg
      361 gaacaaagcc aagctgaact tgctgctatg caatttgttg gcaaatctgc tcaagatctt
      421 gtccaagcga ccttaactgg agataagaaa gttgtcagca ttgatttcaa tccagctgtc
      481 attgacccag aggaccttga aactctttct gatatgaccg ttcaagccat caactctgct
      541 cttgaacaaa tcgatgaaac tactaagaaa aaactgggtg ctttcgctgg gaaattgcca
      601 ttctaaaaac aaggagctag aacaatactt gtcgataaca aaggctaaga aaggtgcaaa
      661 aattataaaa aacgcataat agcaggtgtc aagacactta atattatgcg ttttctcatg
      721 aaaaattctc ttttcatact catttaaaat caaaaaggga aattatcgtt ttacggacat
      781 acatattaca gatttctata ggtcgtgtat agacaggata tcatcgcttg gaagttttcc
      841 tttttgacac caatcagtaa tttagttctc aggaattaat ggcagtgctt ttcgtacata
      901 gtttcataac tacagtatag catagtatat cgctttatag ttttcactat tgatgaactg
      961 cttgagctgc tgtgatgagg gtcaacttgt aaacatcatc tgcattacat ccacgagaaa
     1021 gatcattaac tggcttgttt aaaccttgca aaacaggtcc tacagccgca aagccaccca
     1081 ggcgttcagc catcttgtaa ccgatatttc ctgcctcgat acctgggaag atgaagacat
     1141 ttgcttgacc agctaccgta cttccaggag ctttcagagc tgcagtttca ggaacaaagg
     1201 ctgcatcaaa ttgcaactca ccatcgattt caaggtcagg acgcaagtcg tgagcaattt
     1261 tagttgcttc aacgacctta tcaacgcttt caccaaaccc tgaaccttta gtagaatagc
     1321 tcaacatggc aattttaggt tcgatgccaa acatcttagc tgtgattgct gagttgatgg
     1381 caatttcagc caaggcttct gcatctggat tgatgttaat ggcacagtct ccaaataggt
     1441 aacgttccgt accacgaacc atgaggaagg ctcctgaagt acgagttaca tttggacgag
     1501 ttttgatgat ttgtagagct ggacgaactg ttgaagctgt tgagtgaatc gctcctgaca
     1561 ccattccatc aaccaagccc aagtaaacca acatcacacc aaagtagttg acatcttcaa
     1621 ccaaaacctt gcgtacatct tcttcagtca ttttgccctt gcgacgctcc accaaggcag
     1681 aaaccatttc ttcaaattga ggataatgtt gagggtcgat gacctcataa ccatccatga
     1741 ttccttcaat ttcaagataa attttaattt tttcaggatt tccaagcaaa acaggaatca
     1801 cttctgtttc ttttaccaaa cgtttagtcg cttgaagaat acgtggctct tccccttcag
     1861 ggagaacgat acgagcattt ttaccaacaa ggttggcttt gagactttca aaaacttcca
     1921 tgagttttct cctttaagat aataattata ctctgaaaca gtttttatag agtattagtt
     1981 gataactggg taataaggtt gctgaaatca tccggcaagg gactttctaa ctgcaaatct
     2041 tgctctaaaa atggatgata aaaggatagg taatggcaat gcagggcctg acgttggatg
     2101 ccgtcgtcca gactaccacc gtacaaatca tcgcccaaca aaggaaaacc aatatgagaa
     2161 aagtggactc gaatttggtg ggttcgacca gtgtgcagcc gaatgtcgac caagtggata
     2221 tttccataag aagctacaat cttgtaggaa gtatgggcat attttccacc tttagccacc
     2281 cgtctggtaa taatggagtc ttcatcacgc gcaatcggag caataatttc cccttctggc
     2341 tccaaatgtc catctccctt aaccaaagca aagtagcgtt tctcgataga tttcttctgc
     2401 aactgcttgt ctaatcgtgc atgggcataa ccgtgcttgg caaagagcat caagccagaa
     2461 gtgtccctat ctagtctggt aacaatgtga acctgctgat tttcataatt ttgcttgaca
     2521 tagtaaccct tgataaaatt ggcaatggta ttagagtgat tgacactagg aatagaagcc
     2581 actccatagg gtttattcaa gactagaaag tggtcatcct catagagaat atctaatgga
     2641 agctcaatag cctccaaggt ttcaaagcct ttctcagcgg gaatgtcaat ggtaacgtag
     2701 tctccaacgt ccaatagata cgttgcattt tgcggttgat tattgaccag aatagctcca
     2761 cctcgaaact taatcttggc cagcaatccc ttagaaacct cgtgcttttt taagaaggtc
     2821 ttaaccttga catgttcatc tgcgataaat tcaaacctca ttcatccacc tcaccgataa
     2881 aggcatcctt aacacggttc cagaaactgg tatggctagg agtcgcgaca aagtgaatct
     2941 tatgatgggc gatttgatac tcaatacgct caatattacg gaaagaataa acgctattgt
     3001 caaccgaaat agtatgataa tcgtttcttg ttggaataag ttcaatctta tccttcttag
     3061 gcacaataat ggaagagccc agtgttcgat agacacgatt attaaggctg gcaatttccg
     3121 ttaattgcaa agcttcaatg gtagggtgta aaacagcacc gccaagagac ttgttatagg
     3181 cagtactacc agtcggtgtc gaaactgtta gcccgtctcc acgaaaacgt tcaaagggaa
     3241 caccatttat tacaatatct gccaccatgg ttcgatcaga cctgcggatg ctggcttcgt
     3301 tgagtgctct gaaaatctta acttcaccat tttcaagaaa gaccttcaca ttcagaacag
     3361 ggtaagaaac ccttgcccca gtatctagct gcaaattagt cactagcttg tccaactcaa
     3421 aatcacgata atctgtatag aagcccaaat gtccagtgtg aagaccgata aagcggacct
     3481 tctcaagctg attttcgtac ttatgaaagg ccgacaagag cataccatcc ccgccaatgg
     3541 aaataacaat atccggattg gtatcattga gtataaactg atttctcttc aaacgatctc
     3601 gcaattcata caaaaccctt tgactctgcg gttttctatt ggctatcaga tcaattcgtt
     3661 tacctgtatt cttcatctgt atcgtcactg tttcctacac cgtcatttaa ttttctactc
     3721 aaaggatcaa aaagtgcctg agcttcttgg atatcatcac gaatttcacc catttcttca
     3781 tccaactgat gggcgattct agctgtaatt tccagtcgct tcttaatctc atctgggaaa
     3841 tccccttggt acttgtagtt gagagaatgt tctatcgttg cccagaaatt catggccaaa
     3901 gtacgaattt gaatttctgc caaaatagtc ttaactccat tgatggtatc aaccgtatat
     3961 tctactacca catgatagga acgatagcct gatgcttttc tatgagtaat gtaatctcgc
     4021 tcctgtatga ttcgcatatc ctgacgcttg tgcaaaatat ccactacttc cttgacgtca
     4081 tctacaaact gaaccatcac acgtaagcca gcaatatcct gcaaatcgtg ttccaaggtc
     4141 gcataagtaa tgccacgacg agccattttt tctttgatgc tctcaattgg cttaactcga
     4201 ccggtcacaa actcaattgg agaatgctta ttttgcttac gatattgctt acgaatacca
     4261 cgaagtttaa tctttaactc accaacagct tgaatgtaag gatctagaaa ttcttcccat
     4321 tctaaggtca tatattctcc cttgttctca tattatccgt caaaaatatc tttgattttt
     4381 tctcc
//
LOCUS       DAVMEF010000140         4354 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMEF010000140 DAVMEF010000000
VERSION     DAVMEF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.140"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            271..>4354
                     /locus_tag="VYQ30_001710"
     CDS             271..>4354
                     /locus_tag="VYQ30_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1966779.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGSKAIKELSSLKNTKVLYTYDRIF
                     NGGAIETTQDNLNKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTEV"
BASE COUNT         1684 a          622 c          845 g         1203 t
ORIGIN      
        1 acctgcggct agtttcctag tttgctcttt gattttcatt gagtactaat cttatttttt
       61 aacgtgtgct aaaaaataaa ttaatgcatg ttaatttttt tgacttaaat ttttttaaat
      121 gatatactgt ttttatagag agataacaat tatatataaa tgtactactc tatttcctag
      181 ataatactta gtaaaacttt ttataacaaa ggctagcaaa gttaaagttt attatctatt
      241 ggaagttata taaatatgta aggaattaaa atgaaaaaaa gtacagtatt gtcattaacc
      301 acagctgcag ttattttagc agcctatgcc cctaatgagg tagtcttagc agacacatct
      361 agctctgaag atgctttaag catctctgat aaagaaaaag tagcagaaaa taaagagaaa
      421 cataaagata ttcataatgc tatagaaact tcaaaggata ctgaagaaaa gaaaacaaca
      481 attattgagg aaaaagaagt tgttagtaaa aatcctgtga tagacactaa aactagcaat
      541 gaagaagcaa aaatcaaaga agaaaattcc aataaatccc aaggagatca tacggactca
      601 tttgtgaata aaaacacaga aaatcccaaa aaagaagata aagttgtcta tattgctgaa
      661 tttaaagata aggaatctgg atcaaaagca atcaaggaac tatccagtct taagaataca
      721 aaagttttat atacttatga tagaattttt aacggtggtg ctattgaaac aactcaagat
      781 aatctgaata aaattaaaca aatagaaggt attacatcgg ttgaaagggc acaaaaagtc
      841 caacccatga tgaatcatgc cagaaaggaa attggagttg aggaagctat tgattaccta
      901 aagtctatca atgctccgtt tgggaaaaat tttgatggta gaggtatggt catttcaaat
      961 atcgatactg gaacagatta taggcataag gctatgagaa tcgatgatga tgccaaagcc
     1021 tcaatgagat ttaaaaaaga agacttaaaa ggaactgata aaaattattg gttgagtgat
     1081 aaaatccctc atgcgttcaa ttattataat ggtggcaaaa tcactgtaga aaaatatgat
     1141 gatggaaggg attattttga cccacatggg atgcatattg cagggattct tgctggaaat
     1201 gatactgaac aagatatcaa aaactttaac ggcatagatg gaattgcacc taatgcacaa
     1261 attttctctt acaaaatgta ttctgacgca ggatctgggt ttgcgggtga tgaaacaatg
     1321 tttcatgcta ttgaagattc tatcaaacac aacgttgatg ttgtttcggt atcatctggt
     1381 tttacaggaa caggtcttgt aggtgagaaa tattggcaag ctattagggc attaagaaaa
     1441 gcaggcattc caatggttgt cgctacgggt aactatgcga cttctgcttc aagttcttca
     1501 tgggatttag tagcaaataa tcatctgaaa atgaccgaca ctggaaatgt aacacgaact
     1561 gcagcacatg aagatgcgat agcggtcgct tctgctaaaa atcaaacagt tgagtttgat
     1621 aaagttaaca taggtggaga aagttttaaa tacagaaata taggggcctt tttcgataag
     1681 aataaaatca caacaaatga agatggaaca aaagctccta gtaaattaaa atttgtatat
     1741 ataggcaagg ggcaagacca agatttgata ggtttggatc ttaggggcaa aattgcagta
     1801 atggatagaa tttatacaaa ggatttaaaa aatgctttta aaaaagctat ggataagggt
     1861 gcacgcgcca ttatggttgt aaatactgta aattactaca atagagataa ttggacagag
     1921 cttccagcta tgggatatga agcggatgaa ggtactaaaa gtcaagtgtt ttcaatttca
     1981 ggagatgatg gtgtaaagct atggaacatg attaatccta ataaaaaaac tgaagtcaaa
     2041 agaaataata aagaagattt taaagataaa ttggagcaat actatccaat tgatatggaa
     2101 agttttaatt ccaacaaacc gaatgtaggt gacgaaaaag agattgactt taagtttgca
     2161 cctgacacag acaaagaact ttataaagaa gatatcatcg ttccagcagg atctacatct
     2221 tgggggccaa gaatagattt acttttaaaa cccgatgttt cagcacctgg taaaaatatt
     2281 aaatccacgc ttaatgttat taatggcaaa tcaacttatg gctatatgtc aggaactagt
     2341 atggcgactc caatcgtggc agcttctact gttttgatta gacctaaatt aaaggaaatg
     2401 cttgaaagac ctgtattgaa aaatcttaag ggagatgaca aaatagatct tacaagtctt
     2461 acaaaaattg ccctacaaaa tactgcgcga cctatgatgg atgcaacttc ttggaaagaa
     2521 aaaagtcaat actttgcatc acctagacaa cagggagcag gcctaattaa tgtggccaat
     2581 gctttgagaa atgaagttgt agcaactttc aaaaacactg attctaaagg tttggtaaac
     2641 tcatatggtt ccatttctct taaagaaata aaaggtgata aaaaatactt tacaatcaag
     2701 cttcacaata catcaaacag acctttgact tttaaagttt cagcatcagc gataactaca
     2761 gattctctaa ctgacagatt aaaacttgat gaaacatata aagatgaaaa atctccagat
     2821 ggtaagcaaa ttgttccaga aattcaccca gaaaaagtca aaggagcaaa tatcacattt
     2881 gagcatgata ctttcactat aggcgcaaat tctagctttg atttgaatgc ggttataaat
     2941 gttggagagg ccaaaaacaa aaataaattt gtagaatcat ttattcattt tgagtcagtg
     3001 gaagaaatgg aagctctaaa ctccaacggg aagaaaataa acttccaacc ttctttgtcg
     3061 atgcctctaa tgggatttgc tgggaattgg aaccacgaac caatccttga taaatgggct
     3121 tgggaagaag ggtcaagatc aaaaacactg ggaggttatg atgatgatgg taaaccgaaa
     3181 attccaggaa ccttaaataa gggaattggt ggagaacatg gtatagataa atttaatcca
     3241 gcaggagtta tacaaaatag aaaagataaa aatacaacat ccctggatca aaatccagaa
     3301 ttatttgctt tcaataacca agggatcaac gctccatcat caagtggttc taagattgct
     3361 aacatttatc ctttagattc aaatggaaat cctcaagatg ctcaacttga aagaggatta
     3421 acaccttctc cacttgtatt aagaagtgca gaagaaggat tgatttcaat agtaaataca
     3481 aataaagagg gagaaaatca aagagactta aaagtcattt cgagagaaca ctttattaga
     3541 ggaattttaa attctaaaag caatgatgca aagggaatca aatcatctaa actaaaagtt
     3601 tggggtgact tgaagtggga tggactcatc tataatccta gaggtagaga agaaaatgca
     3661 ccagaaagta aggataatca agatcctgct actaagataa gaggtcaatt tgaaccgatt
     3721 gcagaaggtc aatatttcta taaatttaaa tatagattaa ctaaagatta cccatggcag
     3781 gtttcctata ttcctgtaaa aattgataac accgccccta agattgtttc ggttgatttt
     3841 tcaaatcctg aaaaaattaa gttgattaca aaggatactt atcataaggt aaaagatcag
     3901 tataagaatg aaacgctatt tgcgagagat caaaaagaac atcctgaaaa atttgacgag
     3961 attgcgaacg aagtttggta tgctggcgcc gctcttgtta atgaagatgg agaggttgaa
     4021 aaaaatcttg aagtaactta cgcaggtgag ggtcaaggaa gaaatagaaa actagacaaa
     4081 gacggaaata ccatttatga aattaaaggt gcgggagatt taaggggaaa aatcattgaa
     4141 gtcattgcat tagatggttc tagcaatttc acaaagattc atagaattaa atttgctaat
     4201 caggctgatg aaaaggggat gatttcctat tatctagtag atcctgatca agattcatca
     4261 aaatatcaaa agcttggcga gattgcagaa tctaaattta aaaatttagg aaatggaaaa
     4321 gagggtagtc taaaaaaaga tacaactgag gtag
//
LOCUS       DAVMEF010000141         4251 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMEF010000141 DAVMEF010000000
VERSION     DAVMEF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.141"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            253..2586
                     /locus_tag="VYQ30_001711"
     CDS             253..2586
                     /locus_tag="VYQ30_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1966780.1"
                     /translation="MVEFIKSKEEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2599..4062
                     /locus_tag="VYQ30_001712"
     CDS             2599..4062
                     /locus_tag="VYQ30_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1966781.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4062..>4251
                     /locus_tag="VYQ30_001713"
     CDS             4062..>4251
                     /locus_tag="VYQ30_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEV1966782.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         1487 a          640 c          884 g         1240 t
ORIGIN      
        1 attatttatt tttgaaaaat agctcaccag cccgactgaa agtgcttata aatgataata
       61 agtcgcctgc cgaaaacagc gaaaaatagc ggtgttatgc ggagataatc tgacgcgatg
      121 cgaaagtata ttgcatactt attttcaaca atttagcaga gtatttttat aagtgtgata
      181 taatagaagt ataatttgtt ctgatagttt attttatgga gaagtagatt tttagaatgc
      241 ggagggttca atatggttga gtttataaag tctaaggaag aaatgagtga ggaggatatt
      301 aaagcaaatt tcatcactcc tgctattgta tccaaaggat ggaaaaatgg tgagcatatc
      361 gcttacgaag aatacttcac tgatggtcga attgaggtta gaggagataa ggctcgtcgt
      421 aaagaaggaa aaaaatcaga ctattcactg tattaccaat ttggaactcg aattgcaatt
      481 gttgaggcaa aggataataa acacagcgtt cgagcaggat tacaacaagc tattgaatat
      541 ggagagattt tagatgttcc atttgtttat tcttcgaatg gtgatggctt tattgaacac
      601 gaccgtatca cgagagaaga acgtgagctg gagttagacg aattccctac tcgtgaagaa
      661 ttattttctc gtatgacgaa ggaaaaagga ttgacgtacg aaattacaga agctatctca
      721 actccatact atacagacgc cttctcaatg aaaacgccac gctattatca gcaaatagct
      781 atcaaccgta ctattgaaac agttgccaga ggacaaaaac gagtaatgtt tgtgatggca
      841 acaggaacgg ggaaaacgtt catggctttt caaattattc atcgccttcg aaaagctggt
      901 ttggctaaac gagttttatt cttagcagat agaaacatct tagtagacca aacgatggct
      961 gaagacttta ggccattcga aaaggtaatg acgaaaatta caccaaaact tttgactgct
     1021 cctgaaaaat taaattcttt tgaaatttat ctagggcttt atcagcaact aactggtgaa
     1081 gatggaactg aaacacatta tcaaaaattt gacaaagact tctttgattt aatcgtaatt
     1141 gatgaagcgc accgtggttc agctaaggaa aacagtaact ggcgtaaggt aattgattat
     1201 ttcagttctg cgacacagat tgggatgacc gctactccta aagaaaccaa gaatgcttcc
     1261 aatacggaat actttggtga gccaatctat acttatagtt taaaacaggg aatcgaggat
     1321 ggttttttgg ctccatatcg tgttatgagg gttaatttag atgtggatgt ggatggttat
     1381 cgtccagaaa ctggaaaagt tgatgctaac ggacaattaa tagaagatag gtactacggc
     1441 aggaaagatt ttgataaaac cattgtcatt gatgatagaa cgcaaagagt tgccaagttt
     1501 gtttctgatt atatgaagca aaacaatgca cgatttgata aaacaattgt tttttgtgtt
     1561 gatattgacc atgccgagcg aatgcgtgct gcacttgtaa aagagaatct agacttagtc
     1621 caagaagact atcgttatgt catgcaagta actggtgaca acgctgaagg aaaagctcaa
     1681 ctggataact ttatggatgt caattctaat tttcccgcta ttgtaacaac gtctaaatta
     1741 ttaacgacag gagttaatgc taaaacatgt cgtttgattg ttttagactc taatatccaa
     1801 tccatgactg aatttaaaca aattattggt cgtggcacac gtctttatcc tcaaaagggg
     1861 aaagaatttt ttacgattat tgattttcga aatgttacca atttgtttgc tgaccctgat
     1921 tttgatggtg agccagtgaa ggtgctggaa acaggtgcga aaacagtcag tggttctacg
     1981 cccggtttcg tagatgagga aggtgaccca gtagaaaaat atatcgttac agacaagcag
     2041 gttaccattc ttaattctac tgttcaagta ttggatgaaa acgggaaact gattaccgaa
     2101 agcctgaccg actacactcg gaagaatatc ttaggtagct acgccacttt gaacgatttt
     2161 atcacagttt ggcatacggc ggataaaaag aagcttatct tagacgaact ttataaaaaa
     2221 ggagtttatc tagatgctat tcgagagtcg gagggaatat cagaacaaga aatcgatgat
     2281 tttgatttac tcctaaaact tgcctatggt caaaaagaat taaccaaaac ggaacgtatc
     2341 aataaactca aacaaagcgg atatttatat aaatatagtg aggaagcgcg tgctgttttg
     2401 gaaattttac tgaacaaata catggataaa ggtattggag aactcgaaag cattgaaaca
     2461 ttaaaacttc cagaatttca gatatatggt ggaaccttca aaatcatcaa tacttatttt
     2521 ggagataaaa aacgatattt acaagcaatt aaagaattgg agcaagagct atttacagta
     2581 gcttaatgaa aggaaagtat gtcaattaca tcatttgtaa aaagaattca agatatcact
     2641 cgaaacgatg ctggtgttaa tggtgatgct caacgtattg agcaaatgtc ttggttatta
     2701 ttcttaaaaa tttatgatag ccgtgaaatg gtttgggaat tagaagaaga cgagtatgag
     2761 tcaattatcc cagaggaatt aaaatggcga aattgggctc atgctcaaaa tggggaacgg
     2821 gtattgacag gcgatgaatt acttgatttt gtcaataaca agttattcaa agagttgaaa
     2881 gagcttgaaa taacttcaat tatgcctatt cgaaaaacga ttgttaaatc agcttttgaa
     2941 gatgcgaaca actatatgaa aaatggcgtc ttgttacgcc aagtcatcaa tgttattgat
     3001 gaagttgatt tcaatagccc tgaagatcgt cattcgttta atgatattta cgaaaaaatt
     3061 cttaaagata ttcaaaatgc tgggaactca ggagaatttt atacgccacg tgcagcgact
     3121 gattttattg ccgaagttct taacccaaaa cttggagaat caatggcaga ccttgcttgc
     3181 ggaacaggag gcttcttgac ttcgactctg aaccgtttaa gtagtcaacg taaaactagt
     3241 gaagatacca aaaaatataa tacagctgtt tttggtattg aaaagaaagc atttcctcat
     3301 cttttagcag ttacaaatct gtttcttcac gaaattgatg accctaaaat tgttcatgga
     3361 aatactttgg agaaaaatgt tcgtgaatat acggatgatg aaaaatttga cattattatg
     3421 atgaatccac cttttggagg gtcagaatta gaaacaataa aaaataactt tccagcagaa
     3481 ttacggagtt ctgaaacagc tgatttattt atggctgtca ttatgtatcg tttgaaagaa
     3541 aatggtcgtg ttggagttat tttacctgat ggttttctat ttggtgaagg tgtaaaaact
     3601 cgcttgaaac aaaaactggt agatgagttc aacttgcata cgattattag gttgcctcat
     3661 agtgtctttg caccgtatac aggaatccat acgaacattc ttttctttga taaaacaaag
     3721 aaaacagaag aaacttggtt ttatcgttta gatatgccag atggttataa aaatttctcg
     3781 aaaactaagc cgatgaagtc agaacacttc aatcctgttc gtgactggtg ggaaaatcgt
     3841 gaagagattc tggaaggtaa gttctacaaa tctaaatcat ttacacctag tgaattggct
     3901 gagttgaatt ataatttaga ccagtgtggt tttccaaaag aggaagagga aatcttaaat
     3961 ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca taagattgat
     4021 aatgtattag ctgatatttt gcagttgttg gaggacaaat aatgacacca gaacaactta
     4081 aagcaagtat tctccaaaga gcgatggaag ggaaattagt gccgcaaaat cccaatgacg
     4141 aacctgcaag tgaattatta aagagaatta aagctgaaaa agaaaaactt atcagtgaag
     4201 gaaaaatcaa acgagataaa aaagaaactg agatatttcg tggtgatgat g
//
LOCUS       DAVMEF010000142         4206 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMEF010000142 DAVMEF010000000
VERSION     DAVMEF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.142"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            335..2836
                     /locus_tag="VYQ30_001714"
     CDS             335..2836
                     /locus_tag="VYQ30_001714"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1966783.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEISLADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            3030..3725
                     /locus_tag="VYQ30_001715"
     CDS             3030..3725
                     /locus_tag="VYQ30_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966784.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKTLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLACYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEIWLRPENF
                     DKNGRQHKSLREL"
     gene            3737..4153
                     /locus_tag="VYQ30_001716"
     CDS             3737..4153
                     /locus_tag="VYQ30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966785.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQTEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKEALGNFKEAYQVQ
                     LATEETEKNVGFYRSMGFEILSTYDCTGMIWINREK"
BASE COUNT         1254 a          798 c          982 g         1172 t
ORIGIN      
        1 tgttttagca attgtttcat actatttcag attcagtcca ctatacgttc ttcataaatc
       61 aaaaagcgac ataggttgtc ggctatttat tgtgaataca ttaattagca ttccagtttt
      121 atcttcggtc taaaataagt attttgtgct atacgagata agcttcttga cttactcctt
      181 gatttactgc ataacaatgg gataaaaagt gggagataga gcaattcata gtcatcaaaa
      241 ttaatgagat acagtataca gtttttcctt taaacacatt tcaaattccc tcaaaaatgg
      301 tataatagta acatcacaaa attggagaga gaccatgagt ttttacaatc ataaagaaat
      361 tgagcctaag tggcagggct actgggcaga acatcataca tttaagacag gaacagatgc
      421 atcaaaacct aagttttatg cgcttgatat gttcccttat ccgtctggag ctggtctgca
      481 cgtaggacac ccagaaggtt atactgcaac cgatatcctc agtcgttaca aacgtgcgca
      541 aggctacaat gttcttcacc caatgggttg ggatgctttt ggtttgcctg cagagcaata
      601 cgctatggat actggtaatg acccagcaga atttacagcg gaaaacattg ccaacttcaa
      661 acgtcaaatc aatgcgcttg gattttctta tgactgggat cgtgaagtca acacaacaga
      721 tccaaactac tacaagtgga ctcaatggat tttcaccaag ctttacgaaa aaggcttggc
      781 ctatgaagct gaagtgccag taaactgggt tgaggaattg ggaactgcca ttgccaatga
      841 agaagtgctt cctgacggaa cttctgagcg tggaggctat ccagttgtcc gcaaaccaat
      901 gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc ttgctcaatg acttagatga
      961 actagattgg tcagagtcta tcaaggatat gcaacgcaac tggattggta aatcaactgg
     1021 tgccaatgta actttcaaag taaaaggaac agacaaggaa tttacagtct ttactactcg
     1081 tccagacaca cttttcggtg cgactttcac tgtcttggct cctgaacatg aattagtaga
     1141 cgctatcaca agttcagagc aagcagaagc tgtagcagac tataaacacc aagccagcct
     1201 taagtctgac ttggttcgta cagaccttgc taaggaaaaa actggtgttt ggacaggtgc
     1261 ctatgccatc aaccctgtca atggtaagga aatgccaatc tggattgcgg actatgttct
     1321 tgctagttat ggaacaggtg cggttatggc tgtgcctgcc cacgaccaac gtgactggga
     1381 atttgccaaa caatttgacc ttccaatcgt cgaagtactt gaaggtggaa atgtcgaaga
     1441 agctgcctac acagaggatg gcctgcatgt caattcagac ttcctagatg gattgaacaa
     1501 agaagacgct attgccaaga ttgtggcttg cttggaagaa aaaggctgtg gtcaggagaa
     1561 ggttacctac cgcctccgcg actggctctt tagccgtcaa cgttactggg gtgagccaat
     1621 tccaatcatt cattgggaag atggaacttc aacagctgtt cctgaaactg aattgccgct
     1681 tgtcttgcct gtaaccaagg atatccgtcc ttcaggtact ggtgaaagtc cactagctaa
     1741 cttgacagat tggcttgaag tgactcgtga agatggtgtc aaaggtcgtc gtgaaactaa
     1801 caccatgcca caatgggctg gttcaagctg gtactacctc cgctatattg acccgcacaa
     1861 tactgagaaa ttggctgatg aggacctcct caaacaatgg ttgccagtag atatctacgt
     1921 gggtggtgcg gaacatgctg tacttcactt gctttatgct cgtttctggc ataaattcct
     1981 ctatgacctc ggtgttgttc cgactaagga accattccaa aaactcttta accaagggat
     2041 gattttggga acaagctacc gtgaccaccg tggtgctctt gtggcaaccg acaaggttga
     2101 aaaacgtgat ggttccttct ttcatataga aacaggggaa gagttggagc aagcgccagc
     2161 caagatgtct aaatcgctca agaacgttgt taacccagat gatgtggtgg aacaatacgg
     2221 tgccgatacc cttcgtgttt atgaaatgtt tatgggacca ctcgatgctt cgattgcttg
     2281 gtcagaagaa ggtttggaag gaagccgtaa gttccttgac cgagtttacc gtttgattac
     2341 aagtaaagaa atccttgcgg aaaacaatgg tgctcttgac aaggcttaca acgaaacagt
     2401 caaagctgtt actgagcaaa ttgagtctct caaattcaac acagctattg cccaacttat
     2461 ggtctttgtc aatgctgcta acaaggaaga taagctttat gttgactatg ccaaaggctt
     2521 tattcaattg attgcaccat ttgcacctca cttggcagaa gaactctggc aaacagtcgc
     2581 agaaacaggt gagtcaatct cttatgtagc ttggccaact tgggacgaaa gtaaactggt
     2641 tgaagacgaa atcgaaatcg ttgtccaaat caaaggtaaa gtccgtgcca aactcatggt
     2701 cgctaaagac ctatcacgtg aagaattgca agaaatttct ctagcggatg aaaaagtcaa
     2761 agcagaaatt gacggtaagg aaatcgtgaa agtgattgca gtaccgaata aattggttaa
     2821 tattgttgtg aaataaccaa tttatttgct ggtttcaaat ctcggacagt ttcctaaact
     2881 gtttgagcca gttaaactcg ttaatattgt tttgaaataa gataggagtc cttcagagta
     2941 gaatctggag gattttttga atcttcttat gaaagtatga tatactatgg gcaactataa
     3001 agtttgaaaa gtgaaataag gagaataaga tgccagtaaa tgaatatggt caaatgattg
     3061 gggagtcaat ggaagcttat actccaggtg aattgccttc ttttgatttc ttagaagggc
     3121 gttatgctag gatagaggct ctttcagtgg aaaagcatgc ggaggattta ttagctgttt
     3181 atggccctga tacgcctcgg gagatgtgga cctacctctt tcaggagtca gtagcagaca
     3241 tggaggaact ggtcagcctt ttaaatcaga tgttggctcg taaggaccgt ttttattatg
     3301 caatcataga caaggcaact ggtaagactt tgggaacttt ttccctcatg cgaattgatc
     3361 agaataaccg agtaatagaa gtgggagctg tcactttttc tccagagctc agggggacac
     3421 ggataggaac agaagcccag tatctcttgg cttgctatgt ctttgaggag cttaactatc
     3481 gtcgctatga gtggaaatgc gatgctctta acctgccatc cagacgagca gcggaacgtt
     3541 tgggatttat ttatgaagga accttccgtc aggcagtggt ttataagggg cgtacaagag
     3601 atacggattg gttgtctatg attgataagg actggcctca agtcaaagct cgattggaaa
     3661 tatggttgcg tcctgaaaac tttgataaaa atggacgaca gcacaagagc ttgagagaac
     3721 tttaagaggt gttgagatga ttactattaa aaagcaagaa attgtcaagc tagaggatgt
     3781 tttgcatctc tatcaggctg tcggttggac aaactatacc catcaaacag agatgctgga
     3841 gcaggcctta tctcattcat tagtaattta tctggcactt gatggtgatg ctgtggtggg
     3901 cttgattcgt ttggttggag atggtttttc atcagttttt gtacaggatt tgattgtttt
     3961 gcctagctat cagcgtcaag ggattggtag ctccttgatg aaagaggctt taggaaattt
     4021 taaagaggcc tatcaagtcc agctggcgac agaagagaca gaaaaaaacg tgggatttta
     4081 tcgttctatg ggctttgaaa tcttatccac ctatgactgt acaggaatga tttggataaa
     4141 cagagaaaaa taaaaaaact tgtttgttct taagcaaagt ttaaggatgg tctagtatca
     4201 tatagt
//
LOCUS       DAVMEF010000143         4087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMEF010000143 DAVMEF010000000
VERSION     DAVMEF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.143"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1343)
                     /locus_tag="VYQ30_001717"
     CDS             complement(<1..1343)
                     /locus_tag="VYQ30_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1966786.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATIEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLN"
     gene            complement(1356..3509)
                     /locus_tag="VYQ30_001718"
     CDS             complement(1356..3509)
                     /locus_tag="VYQ30_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1966787.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMIFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVIGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
BASE COUNT         1174 a          993 c          781 g         1139 t
ORIGIN      
        1 ttgttcaaaa attgatccaa ataataacgt agataacttt tcttgcccgt aatcatctgc
       61 aagcggcctt ccaccccgta cctaagtttt tcagcctgct ccgaagttag attagtctcc
      121 gcctctattt taaagaaatt ccctttctca gtcttagtag ctgtcgcatc aatacttgta
      181 atagttgaat ctaggaaaag ttgattcccg gcatcatgag tcgtagtata gcgaacagaa
      241 tcaccgacct tgattcttgc tacatctttt gaacttagat aggctgtgag tttggctttc
      301 ccttctcttt ccaaagatgg ataaagttgg gctagtaggg caccttctgc aaccatgcta
      361 gaatcactgg tctcaggatt aagatgaagc accccatcct cactcgccgt aattttcccc
      421 ttgtctaaaa gatttccctg taccttctta cctgactctg cctccaagat tttctgggct
      481 agaagggtca attcctgacc aacctttgcc aaatgttggg atttaaggga ttccaattga
      541 ctgcttaacc ctgacgcata ggcttgctgg gtacctgaac ctgcatactg gacacggtaa
      601 gtagcaagac tagattctaa ctgagaaatc tgtgcttcaa cctgtgcaac tgcctgaacc
      661 ttagtttggg gattttcctc gccctgagac ttgtaggact ggtaaagaga gtaggctaga
      721 ttctgactgg ccaaggaagc acctgtttcc atagctgact tagctgtctg gtaatcgcga
      781 attttagcct ctgtttgact gatgaggttg ccgatttcgg cttgggtttg gctagctgct
      841 gcattctggg acgcgatggt ctcattttgt tgcgatgtac tagccctaag actgcctgct
      901 tgactgatgt agtcgcgaaa ggtggcttgg tagccaaact tatcctcctc tggaaagtgg
      961 ttctcccctt cttgcaggct cttttgcaga tactctaatt gctttttttg atcctttagc
     1021 atgtccaact gactggcata ggactccgct tggacaccct ctgccccttc ttggtattga
     1081 accaaaagat cccccttctt aaccagctta ttttcttcca aatgattgac aagaatacga
     1141 ttgttgctag ttgactggat atttgcaagg atacgactgg gttcgatagt agctctagtg
     1201 gacaaactca tctccttctc tgcaacagtt gcaaagccaa gtaaaaacac gagcagaagg
     1261 gccatgggta caatcacact actggaaaaa ttatggtaac gacgattata aaactccgca
     1321 ctttctaaaa attctggttt catcctctcc tctttctagc tattgaccaa atgggcgtaa
     1381 aagcctccct gtgcaagcaa atcagcatgc tttccttctt cgacaatctt gccctgatcc
     1441 aagacaacca ccttctctgt ccgctcagca atagtcaagc ggtgagcaat gaaaatcaag
     1501 gtcttgtcca aagccatgag attatcgaca atccgcttct ctgtcaaaat atccaaactg
     1561 ctagtcgcct catccaaaat caagaccggc gcatctgtca agagagcacg cgccaaagcg
     1621 attctctgac gttgaccacc tgaaatccct gccccatccg aagtcaattc tgtctgataa
     1681 ttcagtggca tgcgctcgat atcctctcga atctctgcca attcgaccgc ccgtaagata
     1741 tcttcctgtg tcgtcccctc cttggctccc aaaagaagat tctccaaaat cgttccgtta
     1801 aagacatagg gctgttgagg cagatagttg atgtactggc gcagggcttt tttatcaatc
     1861 tgattgagat tgacaccacc cagactaatc tccccttgac ttgggtcgta aaaattaacc
     1921 atcatcttgg ccaaagtcgt cttacctgac cctgaaatcc ccacaaaagc caccttagac
     1981 ccttggggaa cggttaaatt gatatccgac aagacgtctc gaccatagcc atacttgtaa
     2041 tgaacctgct tgaaggtcat atctcccttc atcaagctca aatcctcaac tgttttcttc
     2101 tcctcaaact cagaagctac tagatacact tcatttagac ggttattggc aacctgcgct
     2161 gtctgaagct tggtttgcag attgatgata ttttccaaag gattggtaaa gtaaaccagc
     2221 aaggtattat aggtaatcaa ctgccccaaa ctcatcttgc catccatgac cagaacagcc
     2281 cccatccaga gaatgccgac attaagcaag agatgggcaa cctttttcag agccttttgc
     2341 tgactctctg ctcgactata ggtaaaggat ttcttcagat aatccacaaa ttccttgtca
     2401 attttttggt aacgctgact ttcactggtc aaggacttga tagtctcaat accgttgatg
     2461 tcctcaatga tagaagaaga cagaaccgca ttggcttcca tggtatcccg attcatcttt
     2521 tcaaacggct tcataaaggc aaagataatc actgtgtaga taggaagcgc caataaagtc
     2581 atgaaaaaga gattggtatt ttgtgaaaat agaacaaggg aaataatgac aaccgttgac
     2641 acatctagga aaatcgaaag gatggtcgaa gccagcgcat cgatgatact gttagcatct
     2701 gtaaaacgag acacgatctc ccctgtcctg cgtgtcgcaa agaaggacat agggaggtga
     2761 aaaacatgct tgatatagga caaaatcacg tcaatcgaca agcgttgccc caaaacaagc
     2821 aagagatact cctgagcgta agacaagatt tgctggagga tgtagacgat gactagccca
     2881 atagaaataa tccctagtgt cgaacgcatc tgatctggca cataggtatc aatgatagac
     2941 tgcagataat aagaacccac aatgttaatc ccgattacca agagtgttgc caaaacgata
     3001 ttggcaatca agccacgctg cttcactaat atagggataa aagagaacag accatttttt
     3061 tgttccttat aaggcttata gtctggacta ggtgccataa aaagagtcac tcctgtccat
     3121 tcttcctcaa aacgctcacg tggcagttta gtcaacttca ccccgggatc tggatcggca
     3181 atatgaatgc tatccttatc ctgcccagtc accacatagt agtggagcaa tttcccttcc
     3241 ttaagcacat gggcaacaaa aggaaaagtc aaatccggca agtcaaaaag cgtcatatcc
     3301 gccttaatgg ctcgcgtctc aaaaccaatc tcctctgcca ccttgaccaa gcccaaagcc
     3361 gtcgtcccat ccatggtcgt cttagccaat tctcgcaagt gagccaaaaa ataataacta
     3421 ccatagtagc caaaaatcat ggctaatgaa gctacaccgc agtccatctg atccacttgc
     3481 ggacgatagt gacgtttccc aaatttcata ttcatctcct tttccctaat actcaatgaa
     3541 aatcaaagag caaactagga aactagccgc aggctgctca aaacactgtt ttgaggttgt
     3601 agataggact gacgaagtca gtcacatata tacggcaagg cgacgttgac gtggtttgaa
     3661 tttgattttc gaagagtata aactcatctg ataacaacta tcttacccca atcactaaac
     3721 aaaaactgga cttccttccc aaatgagcct atctccctcc caactgcact ttttggataa
     3781 aaaaataggc agaacaagag tcctgcccac taaaaatatg ctattgaaag gaaattgatt
     3841 ttttactgtc accctaggca agcttgaaac aaataaatca ttgatagtaa cacttacgac
     3901 atcctattta ttacttcaat ttctaatgtc cctgctattt ctaagctacc tctggtatga
     3961 tctattccta gtcagaatag ataacaccgt tctaaattac gattcatagt tactcctttt
     4021 ttgactcgtt actataagac tcctctttac atgcgcaaaa taattttgat tcagattcat
     4081 catttat
//
LOCUS       DAVMEF010000144         4060 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMEF010000144 DAVMEF010000000
VERSION     DAVMEF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.144"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            596..1588
                     /locus_tag="VYQ30_001719"
     CDS             596..1588
                     /locus_tag="VYQ30_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1966788.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1590..2408
                     /locus_tag="VYQ30_001720"
     CDS             1590..2408
                     /locus_tag="VYQ30_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1966789.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2410..3195
                     /locus_tag="VYQ30_001721"
     CDS             2410..3195
                     /locus_tag="VYQ30_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966790.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLILISLVFFVISLKLWDKGLDSYESAGS"
     gene            3293..3511
                     /locus_tag="VYQ30_001722"
     CDS             3293..3511
                     /locus_tag="VYQ30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1966791.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3496..3642
                     /locus_tag="VYQ30_001723"
     CDS             3496..3642
                     /locus_tag="VYQ30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1966792.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFP"
BASE COUNT         1114 a          707 c          884 g         1355 t
ORIGIN      
        1 cttaagtgtt attttataaa ggttatttca agtcgttcca aggtaacaag tctagatcag
       61 attgaagctg ataaaacgat acaaagaaaa tattcaagtg agctaaaaaa atttattgga
      121 ttttataatg agattatttg tgaggaaaat agtttcctac atgtacgaaa gaggtggtcg
      181 agttggttta ggtagtcgat gcgtgagttg ataattctca gggtatggac ttctttttca
      241 tgaatgaggt aaaagagcag gtattgttta gagacaatca ttctgagcat attttctgga
      301 tagagggagt atccgatttt atgatcaaag ttaataccgc cctctggtga gaagatgagt
      361 aggttggtaa tttaaactat taaacagaat ttttgattaa aagtattatt tcatgagaga
      421 aatcctaatt tcacaatcca taggcaaacg cttgcatttc gttttttatt ggactataat
      481 aggttggtat aaagccttct gtagtaataa aatgtagaag gtgtagaaag taaggattta
      541 gaatatttgt agttaaaaac acaatgttgc tattccttac gatagggaga tagatatggc
      601 aatgatagaa gtggaacatc ttcagaaaaa ttttgtgaag actgttaagg aaccgggctt
      661 gaagggggct ttgcgctcct ttattcatcc tgaaaagcag acctttgaag cggtcaagga
      721 tttgaccttt gaggttccaa aagggcagat tttaggattt atcggggcaa atggtgctgg
      781 gaagtcgaca accattaaaa tgctgacagg aattttgaaa ccaacatctg gtttttgtcg
      841 gattaacggc aagattcccc aggacaatcg gcaagattat gtcaaagata ttggcgtagt
      901 ctttggacaa cgcacccagc tatggtggga tttggctctg caagagacct acactgtctt
      961 aaaagagatt tatgatgtgc cagactcgct ctttcataag cgtatggact ttttgaatga
     1021 agtcttggat ttgaaggact ttatcaagga tcccgtgcgg actctttcac tgggacaacg
     1081 gatgcgggcg gatattgcgg cctccttgct ccacaatccc aaggttcttt ttttagatga
     1141 gccgaccatt ggtttggacg tttcggttaa ggataatatt cgtcgggcaa ttactcagat
     1201 caatcaagag gaagaaacta ccattctttt gaccactcac gatttgagtg atattgagca
     1261 actttgtgat cggattttca tgattgacaa ggggcaagag atttttgatg gaacggtgag
     1321 ccaactcaag gagacctttg gtaagatgaa gactctctct tttgaactgc taccaggtca
     1381 aagtcatctc gtctctcact atgacggtct gtctgatatg accattgata gacaaggaaa
     1441 cagcctcaac attgaatttg atagttctcg ctaccagtca gctgacatta tcaagcaaac
     1501 cctgtctgat tttgaaatcc gcgatttgaa gatggtggat acggatattg aggatattat
     1561 ccgtcgcttc taccgaaagg agctctagga tgatcaaatt gtggagacgt tataaaccct
     1621 ttatcaatgc aggggttcag gagttgatta cttaccgagt caactttatt ctctatcgga
     1681 ttggcgatgt catgggggct tttgtggcct tttatctctg gaaggctgtc tttgattctt
     1741 cgcaagagtc tttgattcag ggcttcagta tggcggatat caccctctac atcatcatga
     1801 gttttgtgac caatcttctg actagatccg attcgtcctt tatgattggg gaggaggtca
     1861 aggatggctc cattatcatg cgtttgttgc gaccagtgca ttttgcggcc tcctatcttt
     1921 tcaccgagct tggttccaag tggttgattt ttatcagcgt tggccttcca tttttaagtg
     1981 tcattgtctt gatgaaaatc atatcgggtc aaggtattgt agaggtgcta ggattaactg
     2041 tcatttatct ttttagctta acgctcgcct atctgattaa ctttttcttt aatatttgct
     2101 ttggattttc agcctttgtg tttaaaaatc tttggggttc caacctactt aagacttcca
     2161 tagtggcttt tatgtcgggg agtttgattc ccttggcatt ctttccaaag gttgtttcag
     2221 atattctctc ctttttgcct ttttcatcct tgatttatac tccagttatg atcattgttg
     2281 gaaaatacga tgccagtcag attcttcagg cactcctttt gcagttcttc tggctcttag
     2341 tgatggtggg attgtctcag ttgatttgga aacgggtcca gtcctttatc accattcaag
     2401 gaggttagta tgaaaaaata tcaacgaatg catctgattt ttatcagaca atacatcaaa
     2461 caaatcatgg aatataaggt ggattttgtg gttggtgtct tgggagtctt tctggctcaa
     2521 ggcttgaatc tcttgtttct caatgtcatc tttcaacata ttccattcct agaaggctgg
     2581 acctttcaag agatagcttt catttatgga ttttccttga ttcccaaggg aatggaccat
     2641 ctcttttttg acaatctctg ggcactaggg caacgcctag tccgaaaagg ggagtttgac
     2701 aagtatctga ctcgtcccat caatcctctc tttcacatcc tagttgaaac ctttcagatt
     2761 gatgccttgg gtgaactctt agtcggtggt attttattgg gaacaacagt gaccagcatt
     2821 gtttggactc ttccaaaatt cctgcttttc ctagtttgta ttccttttgc gaccttgatt
     2881 tatacttctc ttaaaatcgc aacagccagt atcgcctttt ggactaagca gtcaggcgcc
     2941 atgatttaca tcttctatat gttcaatgac tttgctaagt atccgatttc tatttacaat
     3001 tctcttcttc gttggttgat tagctttatc gtgcctttcg cctttacagc ctactatcca
     3061 gctagctatt tcttacagga aaaggatgtg ttctttaacg taggaggttt gatattgatt
     3121 tctctggttt tctttgttat ttcccttaaa ctttgggata agggcttaga ttcctacgaa
     3181 agtgcgggtt cgtaaaagct aaagtaagac taaaatcaag aaaggaactt atgatgtttg
     3241 taattgaaga agtcaaggat gaaaatcaaa aaaggcagtt gtcgctgaga ttttgaagga
     3301 tttgccagaa tggtttggaa tcccagaaag cacacaaacc tatatagaag gaaccacgac
     3361 actgcaagtt tggaccgcct atcaggagag tgatttgact ggatttgtaa gcttatccta
     3421 ttcgagtgaa gattgtgcag agattgattg tctcggcgta aaaaagctta tcaaggtaga
     3481 aaaattggga gccaattgct tgctacttta gagagtgaag ctcgtaaaaa agttggttat
     3541 ctgcaggtca aaacagtggc agaagcttct aataaagatt atgatcgaac aaatgacttt
     3601 tatcgaggtc ttggctttaa aaagttagag atttttcctt aactatggaa tccgcaaaat
     3661 ccttgtcaga ttttgattaa aaagcttgaa taatattact tgacatctat tttttattct
     3721 cagagtgcta tactgtaagt gtaatcgccg atttagctca gttggtagag caaggcactc
     3781 gtaaagccta ggttataggt agataaacga ctgaggagtt gaaaaaatag ataggtagaa
     3841 gataaccgtt aagccttact cttagcggtt atttatattg tttaatagcg ctaatatttc
     3901 atcaattatg cctgttttcg tgtttctggt agttgttcaa gtttattgct actatttttg
     3961 atggtatgaa tgtgcttata atgtatcccg gttaacgaaa gttttggact tatactctta
     4021 gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtgc
//
LOCUS       DAVMEF010000145         3891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVMEF010000145 DAVMEF010000000
VERSION     DAVMEF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.145"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            38..304
                     /locus_tag="VYQ30_001724"
     CDS             38..304
                     /locus_tag="VYQ30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1966793.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            297..665
                     /locus_tag="VYQ30_001725"
     CDS             297..665
                     /locus_tag="VYQ30_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1966794.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            785..2053
                     /locus_tag="VYQ30_001726"
     CDS             785..2053
                     /locus_tag="VYQ30_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1966795.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2050..3327
                     /gene="tilS"
                     /locus_tag="VYQ30_001727"
     CDS             2050..3327
                     /gene="tilS"
                     /locus_tag="VYQ30_001727"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1966796.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3331..3873
                     /gene="hpt"
                     /locus_tag="VYQ30_001728"
     CDS             3331..3873
                     /gene="hpt"
                     /locus_tag="VYQ30_001728"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1966797.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
BASE COUNT         1408 a          541 c          754 g         1188 t
ORIGIN      
        1 gataaaaatg gtacaataat aaattgaggt aataaggatg agattagata aatatttaaa
       61 agtatcgcga attatcaagc gtcgtacagt cgcaaaggaa gtagcagata aaggtagaat
      121 caaggttaat ggaatcttgg ccaaaagttc aacggacttg aaagttaatg accaagttga
      181 aattcgcttt ggcaataagt tgctgcttgt aaaagtacta gagatgaaag atagtacaaa
      241 aaaagaagat gcagcaggaa tgtatgaaat tatcagtgaa acacgggtag aagaaaatgt
      301 ctaaaaatat tgtacaattg aataattctt ttattcaaaa tgaataccaa cgtcgtcgct
      361 acctgatgaa agaacgacaa aaacggaatc gttttatggg aggggtattg attttgatta
      421 tgctattatt tatcttgcca acttttaatt tagcgcagag ttatcagcaa ttactccaaa
      481 gacgtcagca attagcagac ttgcaaactc agtatcaaac tttgagtgat gaaaaggata
      541 aggagacagc atttgctacc aagttgaaag atgaagatta tgctgctaaa tatacacgag
      601 cgaagtacta ttattctaag tcgagggaaa aagtttatac gattcctgac ttgcttcaaa
      661 ggtgataaaa tggaaaattt attagacgta atagagcaat ttttgagttt gtcagatgaa
      721 aagctggaag aattggctga taaaaatcaa ttattgcgtt tacaagaaga aaaggaaagg
      781 aagaatgcgt aaattcttaa ttattttgtt gttaccaagt tttttgacca tttcaaaagt
      841 cgttagcaca gaaaaagaag tcgtctatac ttcgaaagaa atttattacc tttcacaatc
      901 tgactttggt atttatttta gagaaaaatt aagttctccc atggtttatg gagaggttcc
      961 tgtttatgcg aatgaagatt tagtagtgga atctgggaaa ttgactccca aaacaagttt
     1021 tcaaataacc gagtggcgct taaataaaca aggaattcca gtatttaagc tatcaaatca
     1081 tcaatttata gctgcggaca aacgattttt atatgatcaa tcagaggtaa ctccaacaat
     1141 aaaaaaagta tggttagaat ctgactttaa actgtacaat agtccttatg atttaaaaga
     1201 agtgaaatca tccttatcag cttattcgca agtatcaatc gacaagacca tgtttgtaga
     1261 aggaagagaa tttctacata ttgatcaggc tggatgggta gctaaagaat caacttctga
     1321 agaagataat cggatgagta aagttcaaga aatgttatct gaaaaatatc agaaagattc
     1381 tttctctatt tatgttaagc aactgactac tggaaaagaa gctggtatca atcaagatga
     1441 aaagatgtat gcagccagcg ttttgaaact ctcctatctc tattatacgc aagaaaaaat
     1501 aaatgagggt ctttatcagt tagatacgac tgtaaaatac gtatctgcag tcaatgattt
     1561 tccaggttct tataaaccag agggaagtgg tagtcttcct aaaaaagaag ataataaaga
     1621 atattcttta aaggatttaa ttacgaaagt atcaaaagaa tctgataatg tagctcataa
     1681 tctattggga tattacattt caaaccaatc tgatgccaca ttcaaatcca aaatgtctgc
     1741 cattatggga gatgattggg atccaaaaga aaaattgatt tcttctaaga tggccgggaa
     1801 gtttatggaa gctatttata atcaaaatgg atttgtgcta gagtctttga ctaaaacaga
     1861 ttttgatagt cagcgaattg ccaaaggtgt ttctgttaaa gtagctcata aaattggaga
     1921 tgcggatgaa tttaagcatg atacgggtgt tgtctatgca gattctccat ttattctttc
     1981 tattttcact aagaattctg attatgatac gatttctaag atagccaagg atgtttatga
     2041 ggttctaaaa tgagggaaca agatttttta aatcattttc tcaagaaggg atatttcaaa
     2101 aagcatgcta aggcggttct agctctttct ggtggattag attctatgtt tctatttaag
     2161 gtattgtcta cttatcaaaa agagttagag attgaattga ttctggctca tgtgaatcat
     2221 aagcagagaa ttgaatcaga ttgggaagaa aaggaattaa ggaagttggc tgctgaagca
     2281 gaacttccta tttatatcag caatttttca ggagaatttt cagaagcgcg tgcacgaaat
     2341 tttcgttatg atttttttca agaggtcatg aaaaagacag gtgcgacagc tttagtcact
     2401 gcccaccatg ctgatgatca ggtggaaacg attttgatgc gcttgattcg aggaactcgc
     2461 ttgcgctatc tatcaggaat taaggagaag caagtagtcg gagagataga aatcattcgt
     2521 cccttcttgc attttcagaa aaaagacttt ccatcaattt ttcactttga agatacatca
     2581 aatcaggaga atcattattt tcgaaatcgt attcgaaact cttacttacc agaattggaa
     2641 aaagaaaatc ctcgatttag ggatgcaatc ttaggcatcg gcaatgaaat tttagattat
     2701 gatttggcaa tagctgaatt atctaacaat attgatgtgg aaaatttaca gcagttattt
     2761 tcttactctg agtctacaca aagagtttta cttcaaactt atctgaatcg ttttccagat
     2821 ttgaatctta caaaagctca gtttgctgaa gttcagcaga ttttaaaatc taaaagccag
     2881 tatcgtcatc cgattaaaaa tggctatgaa ttgataaaag agtaccaaca gtttcagatt
     2941 tgtaaaatca gtccgcaggc tgatgaaaag gaagatgaac ttgtgttaca ctatcaaaat
     3001 caggtagctt atcaaggata tttattttct tttggacttc cattagaagg tgaattaatt
     3061 caacaaatac ctgtttcacg tgaaacatcc atacacattc gtcatcgaaa aacaggagat
     3121 gttttgattc aaaatgggca tagaaaaaaa ctcagacgtt tatttattga tttgaaaatc
     3181 cctatggaaa agagaaactc tgctcttatt attgagcaat ttggtgaaat tgtctcaatt
     3241 ttgggaattg cgaccaataa tttgagtaaa aaaacgaaaa atgatataat gaacactgta
     3301 ctttatatag aaaaaataga taggtaaaaa atgttagaaa acgatattaa aaaagtcctc
     3361 gtttcacacg atgaaattac agaagcagct aaaaaactag gtgctcaatt aactaaagac
     3421 tatgcaggaa aaaatccaat cttagttggg attttaaaag gatctattcc ttttatggct
     3481 gaattggtca aacatattga tacacatatt gaaatggact tcatgatggt ttctagctac
     3541 catggtggaa cagcaagtag tggtgttatc aatattaaac aagatgtgac tcaagatatc
     3601 aaaggaagac atgttctatt tgtagaagat atcattgata caggtcaaac tttgaagaat
     3661 ttgcgagata tgtttaaaga aagagaagca gcttctgtta aaattgcaac cttgttggat
     3721 aaaccagaag gacgtgttgt agaaattgag gcagactata cctgctttac tatcccaaat
     3781 gagtttgtag taggttatgg tttagactac aaagaaaatt atcgtaatct tccttatatt
     3841 ggagtattga aagaggaagt gtattcaaat tagaaagaat aatctttaat g
//
LOCUS       DAVMEF010000146         3779 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVMEF010000146 DAVMEF010000000
VERSION     DAVMEF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.146"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..1626
                     /locus_tag="VYQ30_001729"
     CDS             4..1626
                     /locus_tag="VYQ30_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1966798.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            1688..>3779
                     /locus_tag="VYQ30_001730"
     CDS             1688..>3779
                     /locus_tag="VYQ30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1966799.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNV"
BASE COUNT         1170 a          688 c          640 g         1281 t
ORIGIN      
        1 atattgctta cagtatctga tgtttcacta cgttttagtg atcgcaaact ttttgatgat
       61 gtcaatatca aatttacaga aggaaatact tacggattaa tcggtgctaa tggtgccgga
      121 aaatcaacct ttttaaaaat tttagctgga gatatcgaac ctactactgg tcacatctct
      181 cttggtccag atgaacgtct ctctgttctt cgtcaaaatc actttgacta cgaagatgaa
      241 cgtgccattg atgtcgttat catgggaaat gaaaaacttt atagcatcat gaaagagaaa
      301 gatgctatct acatgaagga agatttctca gacgaggacg gggttcgtgc tgccgaactc
      361 gaaggagagt ttgccgaact tggaggttgg gaagcagaga gtgaagcctc tcaactactt
      421 caaaacctaa acattccaga agaattacac taccaaaaca tgagcgaatt ggccaacggt
      481 gaaaaagtaa aggttctcct cgccaaagca ctttttggta aaccagatgt tcttctcttg
      541 gacgagccta ctaacggttt ggatatccaa tcgattactt ggttagaaga cttcttaatt
      601 gactttgata acacagttat cgtagtatcc cacgaccgtc acttcttaaa caaagtttgt
      661 actcacatgg ccgaccttga ctttggaaaa atcaaactct atgtcggaaa ctacgacttc
      721 tggaaggaat cttctgagct tgctgctaaa ttgctagcag accgtaatgc taaagcagaa
      781 gaaaaaatta aacaattgca agaattcgtt gctcgtttct ctgccaatgc ttctaagtca
      841 aggcaggcaa catcacgtaa gaaaatgctt gataagattg agctagaaga gattgtacca
      901 tctagtcgta aatatccatt tatcaacttt aaagcggaac gtgagattgg taatgatctc
      961 ttgacagtag aaaatctaac tgtaaagatt gatggtgaaa ctatcttgga taatattagt
     1021 ttcatcttgc gtccagatga taagacagca cttattggac aaaatgatat tcaaacaact
     1081 gcattaattc gtgcaatcat gggagatatt gactatgaag gaactgtcaa gtggggagtt
     1141 acaactagtc aatcttacct accaaaagat aactcagctg attttgcagg aggagaatca
     1201 attcttgact ggttgcgtca attcgcaagt aaagaagaag atgacaatac tttcctacgt
     1261 ggcttcctcg gccgtatgct cttctctgga gatgaagtta acaaacctgt aaatgtcttg
     1321 tcagggggag aaaaagttcg tgtcatgctt tcaaaactca tgctcttaaa atcaaatgtc
     1381 cttgtacttg atgatccaac aaatcacttg gacttggaat ctatctcaag cttgaatgat
     1441 ggattgaaaa actttaaaga atcaatcatc tttgccagcc atgaccacga atttattcaa
     1501 actttggcta accatatcat tgtcttatct aaaaatggcg tcattgaccg tatcgatgaa
     1561 acctacgatg aattcctaga aaatgcagaa gtacaagcaa aagttaaaga actttggaaa
     1621 gactaaataa aacttcaaaa ctcagttggg ttaaccaact gagttttcta tcattctacg
     1681 aggtaacatg aaatcatttt taaaaacata tcgaacctat tttatttctt tcatcattcc
     1741 tgtagtgatt atgtctggag tatatctatc tcaaggtatc tactggaata gcgacaactc
     1801 tcctctatta ggagatggct ttcatcaata cgttattttt gatgtagcct tacgaaatat
     1861 cctacatgga aatagtagtc tgttttacac ctttacaagt ggtctagggc taaacttcta
     1921 tgctctgtct agttattact tgggtagttt tctctcacct ctggtttact tttttgatct
     1981 aacgaatatg ccagatgctg tttatctgac cactctctta aaatttggat tgattggtct
     2041 gtcaaccttt tttagtttga ataaattgtt tcaatctatc cctcagactt taaaactagc
     2101 cttatctact tcctatactc tgatgagttt cactgtcagt caattagaga taaaaacctg
     2161 gctagatgtt tttatcttga ttcctttaat tataactggt ttacatctac tgataactga
     2221 aaagaaactc ctattgtact ttacaagtct gtcaatctta tttattcaaa attattattt
     2281 tggatatatg acagtattgt ttcttatttt ctggtatctc tgtcaaattt cgtgggactt
     2341 taagactcga aaatcatctg ttcttgattt catagttatc tcctttttag ctggtatggc
     2401 tagtttgatt atgactcttc ccactctgtt tgatttacag acacatgggg aaaaattgac
     2461 tgaagttaca aagtttcaaa ctgaaagtag ctggtatctt gatctctttg ctaagcaatt
     2521 cattggttcc tttgacacaa caaagtatgg ggccatccca atgatttttg ttggactact
     2581 tccttttatt ttgaccattt tattttttac gctgaaatct attaagtttc acgtgaaact
     2641 catatatgta ttattctttg catttctaat tgcaagcttt tacatagaag ctcttgactt
     2701 attttggcaa ggcatgcata ctccaaacat gtttttacat cgctatgctt ggattttctc
     2761 taccttgtta atttacacag cagcagaagt cttaaagcgt ctgaaagaac ttaaagtctg
     2821 gaatttttta gtttcgcttt ttcttgtagt aacaggattt ttagctacca tctatctaaa
     2881 atcgcattat tcttttttaa cagatttgaa tattctgctt actcttgaat ttttggttgt
     2941 ctattctctt ttactccttg cagttatcaa aaagtttata tctgtgaatc tatttgccat
     3001 tctaatctct ttatttatac tggttgaaat gagtttaaat gcttcatctc aaatggacgg
     3061 aattgctaag gaatggggat ttgcttctcg aagtacttat agtcgagata tcccagctat
     3121 ggaatctttc tcaacatata ttggaaatca atttactcgt actgaaaaac tacaaactca
     3181 gacaggaaat gacagtatga aattcaacta caatggaatc tctcaatttt catctgttcg
     3241 aaatcgttca tcaagctcta ctttagataa acttgggttt aaatcctctg ggactaatct
     3301 caatctccga tatgcaaata atagtatttt ggctgatagt ttatttggta tccagtacaa
     3361 tatctcagac agtcctattg ataagtatgg ttttaaagat atctatcaaa aagataatct
     3421 taccctatat gaaaatcaat actctcttcc gattgcagtt gctagtcaat ctgtttacaa
     3481 tgatgtcaag ttcaatgaac ataccttgga taatcaggcc tcgtttttaa atcaacttgc
     3541 taacgttgat tttgattatt tttctccaat cccttatgaa aaaatagaaa atactaataa
     3601 tttgattagt gtcacaagtt cttcaaatga ggatgcagca atccagtatc aaattgaagt
     3661 tccagaaaac agccaaattt atctctcttt cataaacctt cacttttcta acgataaaca
     3721 aaagaaggtt gacatccttg taaatggtga aaaaaagact tttacaactg ataatgtct
//
LOCUS       DAVMEF010000147         3744 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVMEF010000147 DAVMEF010000000
VERSION     DAVMEF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.147"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..415
                     /locus_tag="VYQ30_001731"
     CDS             <1..415
                     /locus_tag="VYQ30_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1966800.1"
                     /translation="LDILYKEVGVLSLGQKVVAMLDFLLAYSDYSKDFRLLIIDQPED
                     NLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNGWIESQG
                     YVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1219..1578
                     /locus_tag="VYQ30_001732"
     CDS             1219..1578
                     /locus_tag="VYQ30_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966801.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1588..1953
                     /locus_tag="VYQ30_001733"
     CDS             1588..1953
                     /locus_tag="VYQ30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1966802.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            1940..>3744
                     /locus_tag="VYQ30_001734"
     CDS             1940..>3744
                     /locus_tag="VYQ30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1966803.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLF"
BASE COUNT         1345 a          572 c          758 g         1069 t
ORIGIN      
        1 acttgatatt ctctataaag aagttggtgt attaagtttg ggacaaaaag ttgtagccat
       61 gcttgatttt ttattagcat atagtgatta ttctaaagac ttcagactat tgattattga
      121 tcagcctgaa gacaatctag acaatcgtta tatttacagg catttagttc agcagtttag
      181 agatgtgaaa gctcaacgtc aaattatttt agcaacacat aatgctacaa ttgtaacaaa
      241 ttctatgaca gatcaagttg ttattatgga gtcagatgga gttaacggat ggattgaatc
      301 acagggatat gttagtgaaa aatatataaa aaatcatatc atcaatcaat tagagggagg
      361 aaaagattcc ttcaagcata aaatgtctat atatgagacg gctttatcag agtagagtca
      421 gaaaaagtag gttagaaatt tagcctactt ttttctttgt ccgacaggca tagtgtacat
      481 ctgaggtcca agtcctctgt ggatgtttgc tgcagatgaa accaatagcg actcctaagc
      541 ctgaatatcg tgaggtaggg ggataggaag gaattagcga aatcaaggtt ctacaaacag
      601 aatcgtgact tgaagccata tatagcggat gaggaactct aaaatccaaa taggtgtcgt
      661 aacctatata cgtaaattac gagagtaaac taggaaagat gtacggctta ttccgtgagc
      721 gtttaggacg tagtacaacg aatcatggga gtcagctgaa cacatagtat tgaagaaatt
      781 tctgtaatgg aaatggagcg aagaagtgaa caattaaatg aatacctctc taattaaatt
      841 tgtcaattct aattcctggt atgaaaagac agtgacgatg ggagctgatc ataaatatag
      901 gacggtacat gcagtggtgt tagagattag tccctatttg atttgtgata acttccccaa
      961 atttcttctg ctatactttt ctcaactttt aaaaatccaa ctaagaattt tacctggggg
     1021 tttgggggcg aagccaccaa gttatcttat cgttggctgt caaaactgga aggttttggt
     1081 tagctggcga tatgattttt tgggtattgt gaccacaatg cctgagctcg caaagaccga
     1141 acaagaactt taagaaattt cccgtatgac acacggggtc ggggtttacc cgagaaagga
     1201 tgaaagtgag gaaaagagat gtctgaacag taccgtgaca ttcgaaaaga ggtaaatcta
     1261 accgcagatg agctaaaaca gattgaaaaa atgatggagg ttgataatta tcgtcacttt
     1321 tcaccattcg tcagggataa aattttgatg actgatgaca aacagttagc tgccaaagaa
     1381 tggttttccc tttggcagtc tcaaaagttt gaacagatca gtcgagatgt gcatttggtt
     1441 ttgattatcg caagagagaa tcaccaagtg actcaagaac acgtgtcaat cttattgacc
     1501 tgcgttcaag aattgattgc ggaagtcaat caagtacagt cactcagtcg tgggtttcgt
     1561 gaaaaataca tgagatagga ggctactatg gtttatcgtt atcgtaccaa tctcaaaaaa
     1621 gtatttctaa cagattcaga attacaccaa ttgaatgaac ggattgctaa gagcaattgt
     1681 caaaattttt cagtctatgc cagaaaagtt ttgcttaatc ccaatatgtc atttgtcacg
     1741 attaacacgg atacttatga ccagttagtt tttgaattga gacggatcgg aaataacatt
     1801 aatcaaattg cgcgtgctat caatcaaagc cgtctgattt ctcaggaaca gttacaagaa
     1861 ttgagtaaag gagtcggaga gttgatcaag gaagtggaca aagaatttca agtggaagtg
     1921 aaaagattga aggagtttca tggtagtcac taagcatttt gcgacacacg gaaaaaaata
     1981 tcgtaggcgt ttaattaagt atatcctcaa tcctgataaa acggacaatt tgaaattagt
     2041 atctgatttt ggtatgagca attacttaga ctttcctagc tatgaagaaa tggtagaaat
     2101 gtacaatgtc aactttacca ataatgacaa gttgtacgaa tacagaaatg accgacaaga
     2161 aaaacaccaa caaaatattc atgcccatca cctcattcaa tcattttctc ctgaagataa
     2221 tctgacacct gaagaaatta accgcattgg ttatgagacc attatggaat taacaggagg
     2281 ccgttttcgt tttatcgtag cgactcatac agacaaagat catattcata atcacatctt
     2341 aatcaacgcc attgactgta attcagataa aaagttgata tggaattatg ccttggaacg
     2401 aaaccttcgt atgatttcag accgcatttc taaaatggct ggggcgaaaa ttattgaaaa
     2461 gcgtttctct taccgtgact atcaaaaata tagagcgact agccataagt ttgaactaaa
     2521 gcaacgtctt tattttttga tgcagcagtc aaaatccttt gatgattttt tagaaaaagc
     2581 agagcaatta catgttcata ttgattttag tcagaagcat agtcgattca tgatgacgga
     2641 tcgggcaatg acaaaaccaa ttcgaggacg tcaactcagt aaacgagatt tatatgatga
     2701 agattttttt agaatgcatt ttaccaagca agagattgca agtcgtttag aatttttgtt
     2761 gaactgtgtc aattctttag aagggttact aacaaaatca aaagaattga atctaaccat
     2821 tgacttaaaa caaaaaaatg taatctttat acttgaagaa aatggaaagc aattcagtct
     2881 gagtcataaa aaaataagtg atgagaaatt atatgatgtc aatttttttc aagattattt
     2941 taaaaataag gaagtaggtg tttcagaagg aatagaaaat ctacaggcac agtaccgtgc
     3001 ttttcaagaa gaacgagata aggagaaggt atctactgaa gaaattgagg aagcctttga
     3061 gacatttaag gaaaaacgag atgccgttca tgaatttgaa gtaaaactta cagaacacca
     3121 aattgagaag ttagttgatg aggggattta tatcaaagtg tcctttggta taaatcagag
     3181 tggtcttatt ttcattccta actatcaact ggatattatg gaagaagaaa atcagaaaaa
     3241 atataaagtc tacattcgtg agacaacctc atattttgtc tataacaaag aacattcgga
     3301 taagaatcag tatatcaaag ggagaacctt gattaggcag ctaaccaacg atagtcgagt
     3361 aataccatac agaagaccga ctgttgaaag acttcaagaa aagatttctg agattagcct
     3421 cttgattgag ttaactgaga cagataagaa ataccaagac attaaagaca acttggtttc
     3481 cgaaatagca gagctagata taaaactgac tcaaacaaat gaaaaaattg ccaccttaaa
     3541 taagatggcg gaagtactta tcaattctaa gagtgaaggt tcgggtagcc aaaaactagc
     3601 taggcatgaa ttttctaaat tgaacatgac agaatcaaca actttagaac aagtcaatga
     3661 agaacttttg aagttacaac aggaatttgg taatgttctt gatgagtatg agaaaacaat
     3721 aagaaagctt ggacaattat ttaa
//
LOCUS       DAVMEF010000148         3707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVMEF010000148 DAVMEF010000000
VERSION     DAVMEF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.148"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            18..236
                     /gene="nrdH"
                     /locus_tag="VYQ30_001735"
     CDS             18..236
                     /gene="nrdH"
                     /locus_tag="VYQ30_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1966804.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            317..2476
                     /gene="nrdE"
                     /locus_tag="VYQ30_001736"
     CDS             317..2476
                     /gene="nrdE"
                     /locus_tag="VYQ30_001736"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1966805.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2664..3626
                     /gene="nrdF"
                     /locus_tag="VYQ30_001737"
     CDS             2664..3626
                     /gene="nrdF"
                     /locus_tag="VYQ30_001737"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1966806.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1143 a          805 c          699 g         1060 t
ORIGIN      
        1 tgaagaggag ttacacaatg gtaaccgttt attctaaaaa caattgtgtc caatgtaaaa
       61 tgaccaagcg tttcttggac agtaataatg tctcttatcg tgaaatcaat cttgatgagc
      121 aacctgagta cgtcgatcaa gttaaagagc tcggttttag cgcagctcct gttatccaaa
      181 caccaactga agtcttttca ggtttccaac caggaaaact gaaacaatta gcataatctt
      241 agtacatcat ccagaagaaa ctgcttttag ggctaactta gaagcctttc ttttgtaatt
      301 agataaggga aattttatgg gattaaaaca tcttgaagac gtgacttact tccgtcttaa
      361 taacgaaatt aaccgtcctg ttaatggaca aatcatgctt cataaagata aggaagcctt
      421 ggatgctttc tttaaagaaa atgtagttcc aaacactatg gtttttgatt caatcaaaga
      481 caaaatcaat tacctcattg aacacaacta catcgaaaca gcctttatca agaaataccg
      541 tccagagttc ttggaagaat tggctcaatt tatcaaagac caaaacttcc aattcaagtc
      601 attcatggca gcttataaat tttacaatca atatgcctta aagactaatg acggtgaata
      661 ctatcttgaa aatatggaag accgtgtctt cttcaacgcc ctttatttcg ctgatgggaa
      721 tgaagctgtt gcaatcgata ttgccaatga aatcatccac caacgctacc aacctgctac
      781 tccttccttc ttgaatgctg gacgtgctcg tcgtggggag ttggtatctt gtttcctaat
      841 ccaagtgacg gatgatatga actctatcgg gcgttctatc aactcagctc ttcaactttc
      901 acgtattggt ggtggtgtgg gaattaccct cagcaacctt cgtgaagctg gtgcacctat
      961 caaaggctat gaaggagctg cttcaggtgt cgttcctgtt atgaagcttt ttgaagacag
     1021 cttctcttac tccaaccaat tggggcaacg tcaaggtgcc ggcgttgtct acctcaacgt
     1081 ctttcaccca gatatcatcg ctttcctttc aactaagaaa gaaaacgctg atgaaaaagt
     1141 acgtgtcaag actctatcac ttggtgttgt agtacctgat aaattctacg aattggctcg
     1201 taaaaatgaa gaaatgtacc tcttcagccc ttactctgta gaaaaagaat acggtgtgcc
     1261 attcaattac atcgacatta ctgaaaaata cgatgaattg gtcgcaaatc caaatattcg
     1321 caagacaaaa atcaaggcgc gggatttgga aactgaaatc tctaaattgc aacaagagtc
     1381 tggctatcct tatgtagtca acatcgatac ggctaaccgt gcaaatcctg ttgatggaaa
     1441 gattatcatg agtaacttgt gttctgagat tcttcaagtt caagaaccaa gcttgatcaa
     1501 cgatgctcaa gaattccttc aaatgggaac agatgtttca tgtaacctgg gttcaaccaa
     1561 cgtggttaac atgatgactt cacctgattt tggtcgttct atccgtgcta tggttcgtgc
     1621 ccttactttc gttacagata gttcacatat cgtagctgta ccaacgattg accatggaaa
     1681 tagccaagcc cacacctttg gtctcggtgc catgggactg cacagctacc ttgcccaaca
     1741 actcattgaa tatggttcac ctgagtctgt tgaatttaca agcatctact ttatgcttat
     1801 gaactactgg accttggtag agtcaaacaa tattgcacgt gaacgtggta ttaccttcca
     1861 caactttgaa aaatcagact atgctaacgg aagttacttt gacaagtatg taactggtga
     1921 atttgttcca acatcagacc gtgttaaaga actcttcaaa aatgttttta tccctggtgt
     1981 tgctgattgg gctgaacttc gcgacaaggt tcaagaagat ggtctttacc accaaaatcg
     2041 ccttgctgta gcgccaaatg gttctatcag ctatatcaac gacgtttctg cttctattca
     2101 cccgattacg caacgtatcg aagaacgcca agagaagaaa attggtaaaa tctactaccc
     2161 tgctgctggc ttgtctacag aaaccattcc ttactacact tctgcctacg atatggatat
     2221 gcgtaaagtc atcgatgttt acgctgctgc gactgaacac gtggatcaag gactttcact
     2281 caccctcttc atgcgcagtg acattccaaa aggcctttac gaatggaaga gggaaaataa
     2341 acaaacgaca cgtgatttgt ccatccttcg taactatgcc tttaacaagg gaatcaagtc
     2401 tatctactac gtccgtacct ttacagatga cggtggagaa gtcggcgcca accaatgtga
     2461 aagctgtgtg atttagtttc tagcatagaa acactatttt tccctaagca gcactgataa
     2521 aatcaaatct actatgtcat aacataattt atgtaacatt ttaaaagtcg ggcttccgac
     2581 tttttgtgcg atactcctat agaattatgg taaagtagaa ataattgtga gttcgcaata
     2641 ctaaacacag aaagagattt taaatggaaa cttactacaa agccattaac tggaatgcca
     2701 tcgaagatgt catcgacaaa tcaacttggg aaaaactgac ggagcaattt tggctcgata
     2761 cacgtattcc cttatcaaac gacttggatg actggagaaa gctatctaac aaagagaaag
     2821 acttggtcgg aaaagttttt ggtggtttaa cccttcttga cactatgcaa tctgaaactg
     2881 gggttcaagc ccttcgcgca gacatccgta caccacatga ggaagctgtt ttcaataaca
     2941 tccaatttat ggaatctgtc cacgctaaat cttactcatc aatcttttct accttgaata
     3001 ctaaggctga gattgaagaa attttcgaat ggaccaatac caatccttac ctacaaaaga
     3061 aggctgagat tgtcaacgaa atctacctaa acggcagccc acttgaaaag aaagttgcca
     3121 gcgtcttcct cgaaaccttc ctcttctact ctggtttctt cactcccctc tactatctcg
     3181 gtaacaacaa acttgccaac gttgcagaaa tcattaaatt gattattcgt gacgagtctg
     3241 ttcacggaac ctacattggt tacaaattcc aacttggttt caatgaattg cctgaagaag
     3301 agcaagaaaa actcaaagaa tggatgtacg acctactcta tactctttat gaaaatgaag
     3361 aaggctacac agagagtctt tatgacggtg ttggttggac tgaagaggtt aaaaccttcc
     3421 ttcgctacaa tgccaataaa gctctcatga acatgggaca agatccactt ttcccagatt
     3481 cagctgaaga tgtcaaccca atcgttatga acggtatttc aacaggaact tccaaccatg
     3541 acttcttctc tcaagtcgga aatggttacc ttcttggcga agttgaagcc atgcaagatg
     3601 atgactacaa ctacggtttg gactaatact cttcgaaaat caaattcaaa ccacgtcagc
     3661 ttcgccttgc cgtactcaag tacagcctgc ggctagcttc ctagttt
//
LOCUS       DAVMEF010000149         3631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVMEF010000149 DAVMEF010000000
VERSION     DAVMEF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.149"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(138..3050)
                     /gene="nanA"
                     /locus_tag="VYQ30_001738"
     CDS             complement(138..3050)
                     /gene="nanA"
                     /locus_tag="VYQ30_001738"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1966807.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKENEYFTMSVLDNTALIEGRGANGEQFYDKYTDAPLKVRPGQWNSVTFTVEQPTAE
                     LPHGRVRLYVNGVLSRTSLKSGNFIKDMPDVNQAQLGATKRGNKTVWGSNLQVRNLTV
                     YDRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKERTIREEF"
     gene            complement(3338..3565)
                     /locus_tag="VYQ30_001739"
     CDS             complement(3338..3565)
                     /locus_tag="VYQ30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966808.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
BASE COUNT          953 a          834 c          620 g         1224 t
ORIGIN      
        1 tactatattt tatagaacag actactctga aagtagtttc agacctctta tgatttcgta
       61 tcagcctgaa tgtcatcaaa aaaagatagc aggcttgaaa acctgctatc tccttctatt
      121 tttacaaaat caaatgttta gaattcttct cttattgttc tctctttttt ccctagcgta
      181 aacagaccaa ggaagaaagc tgttagtcct agtgaagcta ggaggtcact ctccttgttt
      241 cctgtttcag gaagtgcctg ctgagcaaga ggagctttgt aagtataatc ttcttttgta
      301 gtaagagtta caagcgaatc agatccctta tactctgcga tttcatgaac agctggctct
      361 gtaccattaa ctccccctgt aaattctgga acttcatgca ccgctgcttt gctaccattt
      421 actccgccag gaactctggc acctgctaga tttacaggaa gattatgcat actttcgatg
      481 cttcctttag ctataccaat aatttcctgt ccgatatctt ccttatctac tgcaaacaac
      541 aaggtcttgc tatcatactg ggttaggaaa gtcgctgttt taccatttgc caattgaagg
      601 gttggagcct tgttgaccaa tacttctgag tcgaactcca agccaataac tcctttgccc
      661 atctctcctt tgcccatctc tctagttcgc ttcactttcg cttcggtagg agaaatcaga
      721 tttttgctca aaaattccca attaaatttt ctaaatgata gggtataggc attttgtcct
      781 ttttcagtat gttcatacaa gatgccatac tccccatttc ctaattcttg gagcgaatta
      841 taggcaaact ctcctttttg aattggattg tgtttgagcc aagtcaactc accattttct
      901 tcgacacgag ccaagtggac catcccattt tcacgtttcg gtccacctgc attgctgagg
      961 atgatgtatt cttttccatt atgcatcgta tggatagcag acatttgaac atagacatct
     1021 ttaacctgtg gataacgttt gatatccttc tcccaagtca ctcctccgtc tttacttgta
     1081 gcaacctgaa gatctccagt caaaccacgc ataaagagtt taacatctcc attgtttagt
     1141 tgtaccaccg ttgattctgt attttgcgca cgtctattgt tcatcgtaga agagtggatc
     1201 ttttgaccgt ctacctgacg gttatcgttg accgcttctc cagcatgcca agtttttcca
     1261 tgatcatctg aatagatgac acgagaagat tgcgagccat ttaagtgaga tacattatta
     1321 gtcgtataaa ccggtatcaa aatccgtccc ttgtgaggcc cattccgaag tacaattcct
     1381 gttccaggac ctacacccaa gaatttcatc caatcggctt tgaccatcgg agtaatatct
     1441 tgaggcgctg accatgtctt cccgtcgtca tcactgtagg acatccatag atagctatcc
     1501 ttggcaattc taaatggaga agttttgttt gttgtgaagt agatattgcc tagtaattgg
     1561 tcacccttgt atagatcacc cttgtcgcta taggctggtt taacaggatc tacaacaacg
     1621 cgatagtctg tcgccttacc atctggtgta tagacagtac cattttctcg aatggtataa
     1681 gctccctttt ctccttcacg atagaggatt tgataggttt ttccatcgat ttttttgtag
     1741 gcttcttctt tttgtgaaga cattccaaag attcccttcc cttctgggaa catgtcatag
     1801 atagaaaaga ttcgtttggt ttcaggatct tgaaccaaca ccatatcgat attcactggt
     1861 gaaccgatcg atgggtcaga agcttttgga ttgtcacgta agttggtaat ggttactcgg
     1921 tcaccccaag ttttaccatt atcttcacta cgtctgatga ccataccgat atcaccccag
     1981 tcactagaat gaagacggcg ttcatctgca cctgcaatca gagttccttt atctgtcttg
     2041 agaagagctg gaatacgata actcttaatt ccatctttat ttggtttgtt gttcattccc
     2101 ccttcaaaga cgtcttcttt ctctgagatt ttcgcacctt ctggaagttt cttctccaaa
     2161 tcacctcttt caaatagttg gcttctcgtc tgaacttctt ctgggcttaa ggcacgatca
     2221 tagacggtta ggtttcgaac ttgtagattg cttccccaaa ctgtcttatt gccccgtttg
     2281 gttgctccaa gttgagcttg attaacatct ggcatatctt tgataaagtt accagatttg
     2341 agacttgttc gagataagac accgttcaca tagagacgaa ctcgtccatg aggtaactcc
     2401 gctgtcggtt gttcgacagt aaaggtcacg gaattccatt gtcctgggcg aactttcaaa
     2461 ggagcatctg tatacttatc atagaactgc tctccgttag cgccacgccc ttcaataaga
     2521 gctgtattat caaggaccga catagtaaag tactcatttt ccttggtgtc actggataca
     2581 gagaagagat tgtaaaagcg tggagctgat gcatctggct taaactccat gtgaactgtt
     2641 gcgttttcaa gtttctttag tttatctagt tcacttgata aatcaactct ctgaccattt
     2701 gaagcattgg tttcaacatc ttctttttct atcactgttt cgacattttc caaatctcgt
     2761 gcatagtaat ctcttggaat tttttcttct tgcttatctt tcctttcttg ttcttgctta
     2821 ttgccagaaa gttcagtctc tgaagaaggc tggctctttt ctgtatcagt tagggttgag
     2881 ctctcccccg aaagttgagt ttcatttgcc agaggttgct cacttgcccc ttcttgagct
     2941 aaaacaggag acgttccaaa taccactgct cctacaatca tagaaaccgc tcctaccgat
     3001 agtttcctaa tgctataacg acacttacgt tcttgaacac tccgattcat actgatcctc
     3061 tctatatcta tatcccgatt tctgaaataa gacatttatt ccagaaaatg cacttagttt
     3121 gtcttttatt aattttcttt tactacagtt catttaaatg acaaaaatga actaaacaaa
     3181 ctatctcaaa ttggtgccaa atttcccctc tcctttctcc aatcagactc ctgccaatct
     3241 agtttatcta ccaggacacc gctgcaatta aactaaacta tctctttata agcaaatgat
     3301 ggacatcagc ttatgctatt tacaccatac ttcctgctca atccttatcg aacacttttt
     3361 caaatagctt ttcataagca taaatacttg tagaaataat gggaaatata atcaagctca
     3421 caatcagaat cttatccagc ttaaagggga caatcccaaa taagtgaaga aataaatcaa
     3481 tcagaaaact gtcgaaaaaa actgtcaaaa gtagttcaac ttgtcttttt ctctgctttt
     3541 ttgtcttgaa tgccttaaat atcatattat tccccttttc taagcttcaa tcccttctct
     3601 ctatctgtta tttcgcatag ctactactat t
//
LOCUS       DAVMEF010000150         3605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVMEF010000150 DAVMEF010000000
VERSION     DAVMEF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.150"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            75..662
                     /locus_tag="VYQ30_001740"
     CDS             75..662
                     /locus_tag="VYQ30_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1966809.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            589..2232
                     /locus_tag="VYQ30_001741"
     CDS             589..2232
                     /locus_tag="VYQ30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1966810.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILEHLTLGGNHMISQEDILKACEVAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2243..3604
                     /locus_tag="VYQ30_001742"
     CDS             2243..3604
                     /locus_tag="VYQ30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1966811.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGIFKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
BASE COUNT         1202 a          733 c          588 g         1082 t
ORIGIN      
        1 attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta tccactatat
       61 gaaaggaatt gccaatgact tcttataaac gtacatttgt tcctcaaata gatgcgagag
      121 actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat ttttctctag
      181 ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt ggcattgtaa
      241 aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa acgctctttg
      301 acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga aaactccaac
      361 attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat cctgaccctt
      421 ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact ggagtagcta
      481 tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat ggtctactaa
      541 gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca
      601 agcttattgg tcactattat caatataggt ggttcttact atctccaagg aatcttggat
      661 gaatacatcc caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc
      721 acctatatcc tccaacaagt catgagcttc tccagagatt atctcctaac cgttctgagc
      781 cagagattaa gcattgatgt gattttatcc tatattcgcc atatttttga acttcccatg
      841 tctttctttg cgacacgtcg tacaggagaa atcatttcac ggttcacaga tgctaactct
      901 attatagatg ccttggcttc taccattctt tctcttttcc tggatgtttc tattctgatt
      961 cttgtagggg gcgtcttact ggcacaaaac cctaatctct tccttctttc tcttatttct
     1021 attcctatat acatgttcat catcttttct tttatgaagc ctttcgaaaa aatgaaccat
     1081 gatgtcatgc aaagtaattc tatggttagc tctgccatta tcgaagatat caacgggatt
     1141 gaaactataa agtcgctcac gagtgaagaa aatcgctatc aaaatataga cagcgaattt
     1201 gtagattatt tggaaaaatc ctttaagctc agtaaatatt ctattttaca aacaagttta
     1261 aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc tcaattagtc
     1321 atgtcgagta aaatttctat cggtcagctg attaccttta acacacttct ttcttacttt
     1381 acaactccta tggaaaatat tatcaacctc caaaccaaac tccaatctgc gaaggttgct
     1441 aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca agaaaaccct
     1501 gttcattcac attttttgat gggcgatatt gaatttgatg acctttctta taagtatggt
     1561 tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga taaggttagc
     1621 ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt caatttcttt
     1681 gaaccctaca aaggacatat ttccatcaat catcaggata ttaaaaacat tgataaaaaa
     1741 gtcttgcgcc gtcatattaa ttacctaccc caacaagcct atatctttaa tggctctatt
     1801 ttggaacact taaccttggg cggtaatcat atgattagtc aagaagatat tctaaaagct
     1861 tgtgaagtag ctgaaatccg tcaagacatt gaaagaatgc ctatgggcta tcaaactcag
     1921 ctctctgatg gagctggtct atcaggagga cagaagcaac gaatcgctct cgctcgtgct
     1981 cttttaacta aatctcctgt tttaatacta gatgaagcta ctagcggtct tgatgtcttg
     2041 actgagaaaa aagttataga taatcttatg tccctaactg ataaaaccat tctctttgta
     2101 gcccatcgtc tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa
     2161 atcattgaag ttggtagcca ccaagagtta atgcaggcgc aaggtttcta ccatcatcta
     2221 ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg agttttatca
     2281 gagacgttac cataactatg cgacagtgtt aattatacct ctttcattac tatttacttt
     2341 catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc aaggagaaat
     2401 cgcccctaca agtgtcattt catctattca gtcaaccagt gataatccta tccttgctaa
     2461 tcatttagtg gcaaatcaag tagttgaaaa aggggactta ctcatcaaat actctgaaac
     2521 aatggaagaa agtcagaaaa ctgccttagc aactcaatta caaagacttg agaagcaaaa
     2581 agaaggactt ggaattttca aacaaagctt agaaaaagcg actgatcttt tttctggcga
     2641 ggatgaattt ggctaccata atacctttat gaattttact aaacaatccc atgatattga
     2701 actgggtatc tcaaagacta acactgaagt ttcaaatcaa gctaatctta ccaatagcag
     2761 ttcatcagcc atcgaacaag aaattacaaa agttcaacaa caaattggag aatatcaaga
     2821 gctgagagat gctatcgtaa ataaaagagc tcgcttacca actggcaatc cgcaccagtc
     2881 aattttaaat cgttatctta tagcctctca agaacaaaca caaggaacgg cagaggagcc
     2941 atttttatct caaattaatc aaagtattgc aggtctggaa tcatctatcg caagcctcaa
     3001 aattcagcaa gctggtatcg gaagtgtagc aacttatgat aatagtttag caaccaaaat
     3061 tgaagtactc cgcactcagt ttttacaaac agcctcacag caacaactaa ccgtggagaa
     3121 tcaattaaca gaattaaaag tacaactaga tcaagcgaca cagcgcttag aaaataatac
     3181 cttaaccgct ccaagtaaag gtatcgttca tctgaacagc gaatttgaag gtaaaaatag
     3241 aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata caagagaagt
     3301 actaatcact tactacgtat cttctgacta tctacctcta ctagataaag gacaaactgt
     3361 aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc aacttcagac
     3421 aattgatcaa actcctacca gaacagagca aggaaatctc tttaaattaa ccgctcttgc
     3481 aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc gcgtcactag
     3541 tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa cacattctga
     3601 ttaat
//
LOCUS       DAVMEF010000151         3601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVMEF010000151 DAVMEF010000000
VERSION     DAVMEF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.151"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1067
                     /locus_tag="VYQ30_001743"
     CDS             <1..1067
                     /locus_tag="VYQ30_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1966812.1"
                     /translation="LKKFSENIGFTTPEEYGAIFKYLIENVKTDRQIIYSPHCHDDLG
                     IAVANSLAAVKNGAGRVEETINGIRERAGNAALEEIAVALNIRQDYYQVETSIVLTET
                     INTSEMVSRFPGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYENITPELVGVKIP
                     LGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKKQEITDADIRALVAGTMVE
                     NPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIFNAIDKFFNQS
                     VRLVSYTIDAITDGIDAQDRVLVTVENRDTETIFNATGLDFDVLKASAIVYINANTFV
                     QKENAGEMGRSVSYHDMPSV"
     gene            1079..2115
                     /gene="leuB"
                     /locus_tag="VYQ30_001744"
                     /pseudo
     CDS             1079..2115
                     /gene="leuB"
                     /locus_tag="VYQ30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            2112..2381
                     /locus_tag="VYQ30_001745"
     CDS             2112..2381
                     /locus_tag="VYQ30_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1966813.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            2385..2744
                     /gene="leuD"
                     /locus_tag="VYQ30_001746"
     CDS             2385..2744
                     /gene="leuD"
                     /locus_tag="VYQ30_001746"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1966814.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            2963..3106
                     /locus_tag="VYQ30_001747"
     CDS             2963..3106
                     /locus_tag="VYQ30_001747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966815.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPKIE
                     AFY"
     gene            3125..3379
                     /locus_tag="VYQ30_001748"
     CDS             3125..3379
                     /locus_tag="VYQ30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966816.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            3369..3530
                     /locus_tag="VYQ30_001749"
     CDS             3369..3530
                     /locus_tag="VYQ30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966817.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQREYY"
BASE COUNT         1107 a          671 c          834 g          989 t
ORIGIN      
        1 tactgaagaa attttcagaa aatataggat ttaccacacc agaggaatat ggtgccatct
       61 tcaaatacct gattgagaat gtcaagacgg atcgtcagat catctattcg cctcactgtc
      121 atgatgacct cggaatagca gtggcaaata gccttgctgc tgtcaagaat ggtgcaggac
      181 gtgttgaaga gactatcaat ggtattaggg agcgagctgg aaatgctgct ttggaagaaa
      241 ttgcagtggc tctcaatatt cgccaagatt actaccaagt agaaaccagt attgtcctaa
      301 ctgagaccat caatacgtca gaaatggttt ctcgcttccc tggtattcca gttcctaaaa
      361 acaaagccgt cgttggtggc aataccttct cccacgaatc tggtattcac caagatggag
      421 tccttaaaaa tcctctcact tatgagaaca tcacacctga attggttggt gttaagattc
      481 cgcttggaaa attatctggt cgccatgctt ttgttgagaa actgagagaa ttggccctag
      541 attttacaga agaggatatc aaaccactct ttgctaagtt caaggcactg gccgataaga
      601 agcaagaaat cacagatgca gatattcgag ctttggtagc tggaaccatg gttgaaaatc
      661 cagaaggctt ccactttgat gatttacaac ttcaaactca tgcagataat gacattgaag
      721 cgctcgttag cctagccaat atggatggtg agaaagtcga atttaatgcg acagggcaag
      781 gttccgttga agcaatcttt aacgctatcg ataagttctt taatcaatct gttcgtttgg
      841 tgtcctacac tatcgatgcg ataacagatg gaatcgatgc ccaggatcgg gttttggtca
      901 ctgttgaaaa cagagataca gaaaccatct ttaatgcaac agggcttgat tttgatgtgt
      961 tgaaggcttc tgctattgtc tatataaacg ctaatacctt tgttcaaaaa gagaatgcag
     1021 gtgagatggg acgcagtgtt tcttaccacg atatgcctag tgtgtaaagg agaaggctat
     1081 ggcaaagaaa atagtagctc tagtaggaga cggaattggc ccagaaatca tggaggctgg
     1141 tttagaagtt ctggaggctc tagctgaaaa acaggttttg actatgagat tgacagacga
     1201 ccgttcggag gtgcagatat tgatgcagca ggacctccct tacctgatga aacccttaag
     1261 gcaagtaggg aagcagatgc tatcctacta gcagctatcg gtagtcctca gtatgatgga
     1321 gcagcggttc gccctgaaca aggcctgatg gctctccgta aggaactcaa tctttacgct
     1381 aatattcgtc ctgtaaaaat ctttgacagt ctcaagtatt tgtcaccact taaaccggaa
     1441 cgaatttctg gtgtagactt tgtcgtggtg cgtgaattga caggcgagat ttactttgga
     1501 gatcatattc ttgaagagcg caaagcgcgt gatatcaacg actatagcta tgaggaagtg
     1561 gagcggatta ttcgcaaagc ctttgaaatt gcaagaaatc gcagaaaaat cgttactagt
     1621 atcgataagc aaaatgttct agcgacctca aaactctggc ggaaagtagc tgaggaagtc
     1681 gcacaggatt tcccagatgt aaccttggaa caccagctgg tagactcagc tgctatgctt
     1741 atgattacca atcctgctaa gtttgatgtt attgtaacgg agaatctttt tggagatatt
     1801 ttatctgatg aatcaagcgt cttatctggt acacttgggg ttatgccatc agccagtcat
     1861 tctgaaaatg gaccaagtct ctatgaacct attcacggtt cagcacctga tattgcaggt
     1921 caaggaattg ccaatcctat ttccatgatt ttatcagttt ccatgatgtt gagagatagt
     1981 ttcggacgtt atgaggatgc agagcgtatc aaacgtgctg ttgagacaag tctggcggca
     2041 ggaattttaa cgagagatat aggaggtcag gcttcaacaa aggaaatgac ggaagctatt
     2101 attgcaaggt tatgaagtta gacgaaaaaa ttactctagt ccttttgatt tggaatgtca
     2161 tcattttctt gatttatgga attgacaaat tcaaggcaag aagaagagct tggcgcatcc
     2221 aagagaaaat cttactcatt ttagccctta cttgtggtgg ttttggggcc tggttagctg
     2281 gaatcacttt tcaccacaag actcgaaaat ggtactttaa aacagtttgg tttcttggga
     2341 tggtgaccac actagtagcc ttatatttta tttggaggta atggatggca gggtcttcga
     2401 gggaacacgc tgcttgggtt ctagcggact atggctttaa ggtcgtgatt gcaggatctt
     2461 tcggtgacat tcattacaat aatgaactca ataatggcct gttgccaatc gttcagccta
     2521 gggaggttag agaaaagcta gcccaactca aaccgaccga ccaagtaact gtagacttgg
     2581 aacaacagaa aattatctca ccagttgaag aattcacctt cgagatagat agcgagtgga
     2641 aacataaact cctaaatagt ttggatgata tcggtattac cttgcagtat gaagagttga
     2701 ttgctgctta tgaaaaacaa cgaccagcct actggcagga ttagaaaaaa tagaaaagga
     2761 aatatagtaa actgaaataa gatgtaaaca aatgaattag agcttaacat ccatttccag
     2821 caatttttta gaaactacag tggactattc tggattcaac acattataaa attatgacaa
     2881 aacacattca caagaaggct acgacatttt aaaaggtgag ggcggatgta tcgtttgccc
     2941 tactaaagtt ggttacatta tcatgaccag tgacaaggca ggacttgagc gtaagttcgc
     3001 agccaaagag cgtaagcgta acaaaccagg tgttgttctc tgcggtagca tggatgaact
     3061 ttgcgcttta gcgcaactca acccaaaaat tgaagcattc tactaaaaac attgggatga
     3121 agatattctt cttggttgta tccttccttg gaaaccagaa gcctttgaaa aactcaaagc
     3181 atacggggat ggccgtgaag aacttatgac tgatgtacgt ggtactagct gttttgttat
     3241 caagtttgga aaagcaggtg aacaattggc tgccaagctt tgggaagaag gtaaaatggt
     3301 ctacgcctca tatgcttcaa tgacaaaacg attgaaactc gctatgagag caaggtgtaa
     3361 tggtgtctat ggtcgataag gacggcaaac tcatcccaga acaaggagga gcacgttcaa
     3421 cctcaccagc accagttgtg atccgtaaag ggcttgacat tgataaaatc atgatgcacc
     3481 tgtcagacac ctttaactca tgggattacc gtcagagaga gtattattag gataaaaaag
     3541 aagtctagtg ttatgagata ttaaagctcc taacacgggg ctttttgttt agaatttctt
     3601 t
//
LOCUS       DAVMEF010000152         3557 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVMEF010000152 DAVMEF010000000
VERSION     DAVMEF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.152"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..1429
                     /locus_tag="VYQ30_001750"
     CDS             20..1429
                     /locus_tag="VYQ30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1966818.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1447..2742
                     /locus_tag="VYQ30_001751"
     CDS             1447..2742
                     /locus_tag="VYQ30_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1966819.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2747..3055
                     /locus_tag="VYQ30_001752"
     CDS             2747..3055
                     /locus_tag="VYQ30_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1966820.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3052..3360
                     /locus_tag="VYQ30_001753"
     CDS             3052..3360
                     /locus_tag="VYQ30_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1966821.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1193 a          605 c          698 g         1061 t
ORIGIN      
        1 aatagaaagg aggttgagca tgaaaaaaaa gttagtattt cctaatctgt tttggtgggg
       61 agctgcttct agcggacctc agacagaagg tcaatatgga aaagtacatg aaaatgtgat
      121 ggactactgg ttcaaaacgc atccagaaga ttttttcgat aatgtcggac ctcttgtagc
      181 cagtaacttt tttcatactt acaccgaaga tttccacttg atgaaggaaa ttggagttaa
      241 ttctttccgc acttccatcc aatggagtcg actcatcaag aatttagaga caggtgagcc
      301 tgatccaaaa ggtattgctt tctacaatgc catcattgaa gaagctaaaa agaaccagat
      361 ggatcttgtg atgaatttac atcattttga tttaccagtg gaacttcttc aaaaatacgg
      421 tggttgggaa agcaaacatg tagtggagtt attcgtgaag tttgccaaga ctgctttcac
      481 atgctttgga gataaggttc attactggac aactttcaat gagccaatgg tcattccaga
      541 agcaggatac ttatatgctt tccattatcc aaatctaaaa ggaaagggaa aagaggccgt
      601 acaagtcatc tataatctaa accttgctag tgcaaaagtg attcaactat atcgctcatt
      661 aggacttgat ggaaagattg ggattatttt aaacttgaca cctgcttatc caagaagtaa
      721 ttctccagaa gacttagaag caagtcgatt tacagaagac ttctttaaca aagtcttctt
      781 gaatccagct gttaaaggaa ctttcccaga aagattggta aaacagctag agagagatgg
      841 cgtgttatgg agtcataccg aaaaagagct tcaactgatg aaatcaaata cggttgattt
      901 tcttggagta aactactacc atccaaaacg tgttcaagca caagcaaatc ctgaggaata
      961 tcagacgccc tggatgccag accaatactt caaagagtat gaatggctgg agcgtcgcat
     1021 gaatccatat cgtggttggg aaatttttcc gaaagccatt tatgatattg ctatgattgt
     1081 gaaggaagaa tatggtaata tcccatggtt tatcagtgaa aacggaatgg gtgttgaaaa
     1141 cgaagcacgg tttatcgatg aaaatggagt tatcgatgac gtgtatcgta ttgaatttta
     1201 tgaagaacat ttaagatggc tacataaagc cattgaagag ggaagtcact gttttggata
     1261 ccacgcttgg accgcatttg attgctggtc ttggaataat gcatataaga atcgttacgg
     1321 atttatctcc gttgatttag aaacgcaaaa gagaaccatc aagagctcag gaagatggta
     1381 tcgcaaagta agtgacaata acggttttga agtagaaatt gaggagtaaa gagaggagat
     1441 attcatatga aaaactcaaa atttatagac caatttgcca cctttgctgg taaactaggg
     1501 aaccaaattc atttaaaaac cctaagagat gcattcgtaa cagtaatgcc attatatatt
     1561 ttggcaggtt taatcgttct tttgaacaac acggtattta agtggatttt ccaaggggat
     1621 acattaacaa gattccaata ttggggaata acaattgcaa acggtacttt aagtatttca
     1681 ggtatgatta ttgctgtaat ggttggttat ttcttagcta aaaacagaga tttcgaaaac
     1741 ccgttagcag catcaatgct atcattagtt tctttaattg tgatgatgcc aaatacagtt
     1801 tctgtagttc ctgacggagc aaaagatgcg gtaaacattt caggtgttct ttcattcaac
     1861 aacacaggta caggcgcaat gttcgccggg gttatcgtag cgattattgc aacagaatta
     1921 ttcattgaat tatcaaatgt taaagcttta caaatgaacc ttggtgaaaa tattccacca
     1981 gctgttagta gatcatttag cgtattactt ccagtcatga ccgtcatctc cttatttggg
     2041 gttgtttcag cattattatt caatataact ggaatgaact taatctcaat cattacaatc
     2101 tttattcaag aaccaattcg tcatattggt acaagcttaa tcggggtcat tattatttac
     2161 tctttaggaa atatgttatg gctatttggt attcaccaag cagttattta cagtgccatc
     2221 ctagaaccat tactattaat taacattact gaaaacatca ctgcagcaaa taatggacaa
     2281 gccattccac acatcatcaa cctatcacaa atacaaacat tcgctttaat gggtggtagt
     2341 ggatctacat tatgtttatt aatagcaaca ttcttagtga gtcgcaatgc tgtctctaaa
     2401 aacgtggcta aattatcttt tggacctggt atcttcaata tcaatgaacc agtattattc
     2461 ggttacccaa tcgtttataa catttcatta gctattccat ttatcacagt tccagtcctt
     2521 ggtattttaa tcagctactt agcaacagtt acaggattca tgagtcctgc atttatacaa
     2581 gttccttgga ctacaccagt attcttaaat gcatggttag caacagcagg ggacgtgaga
     2641 gcagttctag ttcaattcat catctttgca cttggagttc ttctatacat tccatttatc
     2701 aaagttaatg acaaagttgt tgaacaagaa atggaaggtt aagattatga aacaaatttt
     2761 attagtatgt aatgcgggaa tgtcgacaag tatgcttgtt aaaaaaatgc aacaaagtgc
     2821 gactgaacgt gggattgaaa tctctatcca agcaaaatca atgacagaag ctaaaaagaa
     2881 tatccatgaa gcagatgtca ttctaattgg gccacaaatt cgttatgagt tacttgctgt
     2941 gaaagaaatt gcaggaaata tccctgtaga taccattgat atgcgggatt acggaatgat
     3001 gaatggagca aaagttctgg aacaagcact tgcatggata ggagaaataa gatgacagaa
     3061 gaaatggaat atatctgttt tcagctcatt gccaatagtg gagcagccaa atcatccttt
     3121 attgaagcca tccaactagc taaggcagga aatttaaaag aagcgaaaat taaagttgaa
     3181 gaagcggaag actccttagt agaagcgcac aagattcact ctaatctcat acaaaaagaa
     3241 gctactggag aaaaaatagg attctccttg ctatttatgc atgcagaaga ccaaatggct
     3301 tcaacagaga ttattcagtt gctatctaaa gaattcatcg atctctatca aaataaatag
     3361 gaacactgtc gttcccatct gtctttatta atgacggcta cattgtagga acctccaaaa
     3421 gttggacaaa atgattgaaa ggattggatc ctgcattaca caagatccaa tccttttctt
     3481 ttactctagt ttttataatg tgttgaattt agaatagtcc actgcagttt ctaaaaaatt
     3541 cctggaaata gatgtta
//
LOCUS       DAVMEF010000153         3532 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVMEF010000153 DAVMEF010000000
VERSION     DAVMEF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.153"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(336..917)
                     /gene="pdxT"
                     /locus_tag="VYQ30_001754"
     CDS             complement(336..917)
                     /gene="pdxT"
                     /locus_tag="VYQ30_001754"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1966822.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQDMLIPIREAILSGLPAFGTCAGLILLAKEIASQEESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGQIPMTFIRGPIISSVGEDVEILARVDNQIVA
                     AQEKNMLVTSFHPELTDDVRLHQYFINMCKEKR"
     gene            complement(918..1793)
                     /gene="pdxS"
                     /locus_tag="VYQ30_001755"
     CDS             complement(918..1793)
                     /gene="pdxS"
                     /locus_tag="VYQ30_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1966823.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(1945..3324)
                     /locus_tag="VYQ30_001756"
     CDS             complement(1945..3324)
                     /locus_tag="VYQ30_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1966824.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
BASE COUNT         1034 a          793 c          631 g         1074 t
ORIGIN      
        1 gtacagctat tatatacaaa atttaaacat aaagcttagg tatacttaat aaatgagaaa
       61 taaagttagc gataacgaag atagtagcat atgtaaaact ctaagcaggc agagcctgct
      121 tagagtttta caatcgaatt tcccaaagag gaagcaagat gaaagccgtc attgcttatg
      181 ctcataagat tctcagaatc gtttacaaac tactctccac caagcatact tatcaaaaag
      241 aaaagacgct aggactgagg aaacagttct aacgcccaaa ctaaaaaaat ttcaattaca
      301 gtatagcaca ggaagtgatt ttttgcgctt ttttactatc ttttttcttt acacatattg
      361 ataaagtact ggtgcaagcg aacatcatca gtcaattctg gatgaaaaga agttaccaac
      421 atatttttct cttgggctgc aacgatttga ttatcaactc ttgctagaat ttctacatcc
      481 tctccaacac tactaataat cggaccacgg ataaaggtca ttggaatctg accaactcct
      541 ttacattctg cttccgtata gaaacttcct aattggcgcc cataggcatt gcgctcgacc
      601 accatatcca tagttcctag atgactctct tcctgagaag caatttcctt agccagcaaa
      661 atcaagcctg cacaggttcc aaaggctggt aaaccagata gaatggcttc tcgaatgggg
      721 atcagcatgt cctggtctcg taagagcttc cccatggttg tagactcacc accaggcaaa
      781 atcaaacccg acaagtcact ctgatcttgc tgaaaatcat ctagatttct gagttctaca
      841 ctctcgacac ctaattgatc tagcactttt gcatgttctg caaaggcccc ttgcaaggcc
      901 aatattccga ttttcatcta ttttcctcgt tcagccatga gaatttggat ttcattttca
      961 ttaataccaa ccatggcttc tcctaaatct tcagagattt gagctaggat ttgaggatta
     1021 cggaagttag tcacagcctt aacaatggca ctcgctcgtt taacaggatc tcctgacttg
     1081 aaaatacctg aaccgacaaa gaccccctct gcccctagtt gcatcattaa cgcagcatct
     1141 gctggcgttg caacacctcc agcagcgaaa tttacaactg gcaattttcc atgttcatga
     1201 acatattgga ccaattctac agggacttgc aaatccttgg cagcaacata aagctcgtcc
     1261 tcacgtaagt tttgaatgcg gcgaatttcc tgattcatca tacgcatatg acgaacagct
     1321 tggacgatat cccctgtccc tggttctcct ttggtacgaa tcatggaagc accttcagcg
     1381 atacgacgca aggcttcacc caaatcctta gcaccacaga caaaaggaac ttggaattct
     1441 ttcttgtcca catggaaacg gtcatcagct ggagatagaa cttcactctc gtcgatataa
     1501 tcaatttcaa tagcctctaa aatctgagct tcaacaaaat gcccgattct gaccttagcc
     1561 attactggaa tactaaccgc ttcttggatt tccttaatca tctttgggtc gctcatgcgg
     1621 gaaactcctc cagctgcacg aatatcagcc ggaattcgtt ccaaggccat cacagctgcc
     1681 gcaccagcag cttctgcgat acgagcctgt tcaggattct gcacatccat aataacacca
     1741 cccttgagca tctgtgccaa gtttttattt agttcataac gattttcagt catttgtttt
     1801 atcctcatca tttgtattgg tagctacctt ttcattttaa gtcagaacag aatgaagcac
     1861 aagataaaaa aactataatt gtatggggac agattagcta aaaactatct gaccttaact
     1921 taaggccaga tagctcattc atttttattt ttcagccgta agggcagcca ttgtgatgta
     1981 gttgtatggt ttgttgaagt gtggcaagaa gaagaggtct gtcaatgcca atttatcaat
     2041 tgtcacatgc tcttggatag caagtgagaa catgtggatt cccatgctaa ttgcaatatc
     2101 atgggaaacc atttgggcac caagaatttc acggctatct ttgtcaaaga caatcttaat
     2161 tgctacttca tggttgtcat gtttcatgaa ttctggtttt tgaagatcgt taaagcctgt
     2221 ttcagttgcg ttgtaaccag cagctttcgc tttttcaaga gtcaaaccag ttgaaaccat
     2281 gtgaagaccg tagattgaga taccatttga accttgaaca ccgattcctt ccaattcatg
     2341 tccacaagcg ttgtaggcac caacgatacc agtgcgaaca gcatttgaag caagagcgat
     2401 atagcttgta tctttacgag cattgtcata aacagtcgca cagtcaccaa cagcgtaaac
     2461 gcctgggata gatgtttctt gtttcttgtc tacaaggaag gcaccgttgc ggaagagttc
     2521 gatcttacca tcagcaaggg ctgtgtttgg acggaaacca actgcaagga taaccatatc
     2581 cacgtcaaag ctttctttgt cagtaatcaa gcgttcaact ttaccgtcac cttcgattgc
     2641 tttaacagtt tgacctagag ccaagcggat gttgtgatct tccaagttct tcgccatcat
     2701 ttgtgtgaag tctttgtcat agtaaccgtt caagacagta tcaacgatat caacaaggac
     2761 aacttctttt ccaagacgtt caaaggcttc agcaagttca acaccgatgt aaccaccacc
     2821 aacaacggcg atacggtcga ggtgttggct cttgtcagaa agtttattga taacttcttc
     2881 agcattttgg tacaatttca cgaattgtac gttttcaaga gttgctttaa attcgcggtt
     2941 tcctttaaca atttcaacac cttcgattgg tggcaagatt ggtgtagaac ctgtagcgaa
     3001 aatcaatttt tcgtatgatt ctttgtgctc ttttccttca acttccgctg taactacttt
     3061 gttatcatag tcgattgaaa gaacaggtga gttcatgtaa actttagcac ctttagcttc
     3121 caatttttct ttatcagaat agaacaagcc ttcagcaccg tcaatttgtt caccaatcca
     3181 aagagccatt ccacatccta ggaaagagat gttagagttt tggtcaaata caacaatttc
     3241 gttctcattt ccaaaattat ccaacatggt attgatacat gctgtaccag cgtggttagc
     3301 accgactaca acgattttac tcataaaaat ttcctgcctt cattctaaat ttaccttact
     3361 agtataccat ttaccgttag cgtttacaag gggggtacta ttatttcagc attttttatc
     3421 aaaaataatt ccccatttat cctgttttca agcctcatga attttatgag catatttctt
     3481 gactttttcc aaattttgtt tgtgaaaatt ctaactttat ggtattataa ta
//
LOCUS       DAVMEF010000154         3528 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVMEF010000154 DAVMEF010000000
VERSION     DAVMEF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.154"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(54..764)
                     /locus_tag="VYQ30_001757"
     CDS             complement(54..764)
                     /locus_tag="VYQ30_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1966825.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(767..2458)
                     /locus_tag="VYQ30_001758"
     CDS             complement(767..2458)
                     /locus_tag="VYQ30_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1966826.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(2470..3480)
                     /locus_tag="VYQ30_001759"
     CDS             complement(2470..3480)
                     /locus_tag="VYQ30_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966827.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
BASE COUNT         1258 a          677 c          510 g         1083 t
ORIGIN      
        1 aaacatctaa aaaacaaaag gacaaaaaca tctgtccctt tgtttactaa atttcagcta
       61 atttcttcga cataaataac acctacaata ttagcaattt cttccatcag tcgaagatgt
      121 tcaaatctac ctgataattc cagagtaata aatgacgcta tttttttgtc cggaacatca
      181 aagtattcaa ttctgtcaga attaacatct ccaaacgctg ttcttgaatc ggtcattctg
      241 ataccatttt ctgcacaata aaccaataca cgattatagg cttctgtaga tttaactact
      301 atatacaatt caatcatttt agaacgattt tgcagatatt tttttagtgg ttggaacatg
      361 gatatcacac cccaaacaga aatggctact aaaagagctc cctcataaaa acctactcca
      421 atagctaatc caattcctgc cgaagcccaa atgcctgctg cagttgtcag acctgtaatt
      481 ttctttttat ctgtaataag aatcgttcca gcgcctagaa aacccacacc tgatataact
      541 tgagctccta atcgtgtagg atctcctgtc ccaaatttat aagatacgta ttcattcgtc
      601 atcataatca aacatgcagc tagacaaaca atactataag ttcggatgcc tgcaggctgg
      661 gatttgctcc ctctctctaa accaattata ctaccaatga ctactgataa aacaatcctg
      721 acaactattt caatatttga taacccaaga ctagtggctg tcatgattat ttccttactt
      781 tacgccccgg tctttgtgtg aagtataata ccgttccaga aataatcatc agaacaattg
      841 tataaacaaa taccagagct tgtgcattag atgttgctgt ttcatcacct gcagatcgaa
      901 tcgtaatacc taatggttga gctaggggat ggtaaaggaa tacagataag tcgaagtcag
      961 ttaataaaga gttaaagttt aaagcaataa cagagagaac aaccggtaaa ataaatggaa
     1021 tgataacctt catcatagta taaaaaggtg aagcacccat acttcttgct gcatcttcca
     1081 tctcatcatc aacactaaat aaaatagcac gtaccattct ataagaaaat gggattttta
     1141 caactatata tgcaataagt agaattacca aactacctac caaaatctga ttcaagacaa
     1201 gaaattgtgg ctgattaaaa gtaaataata aacttactgc taaaagtgta cttggtagta
     1261 accaaggaag tagagcacca tattcaaata agaaatcaaa acgagattta tgttttctga
     1321 caacacgagc aaatacaact gcgagaattg ttgctgttgt cgcagcaata atagaataaa
     1381 taaagctgac caagaatgga gagaatgccg cactattact aaagaataag cgataatttt
     1441 ctaaagtaaa gtttgataat gttaagttac ctgtttgaat tgcaactgga tctgtaaatg
     1501 agtataatac tataaaaatt agtggaagca tgaaaactgt gaacaatcca tatgctacaa
     1561 tgtgagcaat gatattccaa ggcttagacg caattttttg ttttttaaga ggcgctttag
     1621 tcttagagat agaaatataa tttccacctt tttctatctt attcatgata gtaagcaaaa
     1681 ttgtagttgc aatacctaaa ataattgcaa gtaaggcagc taaatcacga gaattcccca
     1741 tccctgcaaa tgtaataatc attggattta tagtttgaaa ttctttacca ccaacaatca
     1801 tgggtgctgc tactgcagat aaaccactaa gaaaaaccat aatagtaagt gcaaatagag
     1861 ttggaattaa ggttggtaac actacttttc ggaaaacagt aaatggtttt gctcccatat
     1921 ttcgagcagc ctcaatagtg tgatagtcaa cgcttcgaat tgtatttgtt aaaaacaatg
     1981 tatgattagc agttcctgaa aatgtcataa tgaataagac tgcaccatac ccaataaacc
     2041 agttagagtc taaagaaggg ataacatttt gtaaaaattt tgtaatcaat ccataaggac
     2101 catagacaaa tttatatcca gtcgctaaaa ccactcctcc ataaattaaa gaggtcatat
     2161 aacctaattt taaaatttta gcacctttaa tatcaaagta ctctgtgaat agaacacaaa
     2221 gaatacctac gacattaact gtaataatga gtgaaaatgc taacttaaaa ctgttcataa
     2281 tactctgaag tgccctctga gattttagaa cacgatgtac agcatcaagg gaaaattctc
     2341 ctccttttac aaatacattc actactagat caaagtttgg ataaataata aatgttacta
     2401 agaaccagat taaccctaaa cgaataagcc aatcttttaa atttaattta tgacgcatac
     2461 tgcacctcct taaaattgca gaacgtctga tggtgtgata aataattcca cactttctcc
     2521 gacagatcta atagcagcct gactatcaat acttgttaca ttaagaatct gactttcaga
     2581 aacttttatt gtatagtgaa ttgtaactcc agaaaactca acatcaataa ttgtcccttt
     2641 tagaataaaa tcttgttcag tttcacgatt gaatcgaact ttctctaatc gaatgtatcc
     2701 ttttttatcc tctaaaaaaa cgcttgtatt tttcaataat acttcgtgga ctgtttcatc
     2761 ggtcaaaaca ttaatatctc caataaaatc acatacaaat tcagtttgag aattatgata
     2821 aatctctact ggtgtaccga cctgttcgat gtatccattg ttaaagactg caattctatc
     2881 agataaagtc aaggcttcct cttgatcatg agtaacatat aaagtagtaa tacctaactc
     2941 tttttgaagt cttttcaact cttttctcaa atctacacgt aattttgcgt caaggtttga
     3001 caatggttca tctagacaaa gaattttagg ttcaagaacc agagcacgag ccaatgctac
     3061 cctttgttgt tgacccccag ataattctga tacattacgc tgtaactgtt gatcagagat
     3121 cttaattttt gctgccactg ctgatacttt agctttaata acatctggag ctaccttctt
     3181 aacttttaaa ccaaatgcaa tattatcaaa aacagtcata gttggaaata gcgcataaga
     3241 ttgaaataca ataccaattc cacgcttttc aggttccaaa tgagtgacat ctgttccatt
     3301 aacttcaata cttcctgatg atggatctaa aaaacctacc aatgctctca aagtagttga
     3361 tttaccacat cctgaaggcc caagaaatgt aaagaattcc ccttcatgta tatctaaatt
     3421 cagattatca attgcaacaa aatcaccata tttaatttga atattatcaa atttaatcat
     3481 ctcactaact ccctctatta ctaaaaccaa aagcctctct ttatttct
//
LOCUS       DAVMEF010000155         3493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVMEF010000155 DAVMEF010000000
VERSION     DAVMEF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.155"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            138..1628
                     /gene="lysS"
                     /locus_tag="VYQ30_001760"
     CDS             138..1628
                     /gene="lysS"
                     /locus_tag="VYQ30_001760"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1966828.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKLPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            2253..3389
                     /gene="lctO"
                     /locus_tag="VYQ30_001761"
     CDS             2253..3389
                     /gene="lctO"
                     /locus_tag="VYQ30_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1966829.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         1065 a          692 c          745 g          991 t
ORIGIN      
        1 tttccagtga ttttagcgtc aggttctttt tagtttttaa agattatccg tgaatttctt
       61 gcttatttat gataaaatgg gagtatcgca aaaaatgact catcgtattc aattttgagt
      121 aaaactagga ggatcccatg tctacagaac atatggaaga actaaatgac cagcagatcg
      181 ttcgccgtga aaaaatggct gcgctccgcg aacaaggaat cgatcctttc ggaaaacgtt
      241 ttgaacgtac tgcaaattca caagaattaa aagataaata tgccaacctc gataaagaac
      301 aattacacga taaaaacgaa acagctacta tcgcaggacg cttgataacc aaacgtggta
      361 aaggaaaagt tggttttgcc caccttcaag accgcgaggg ccagattcag atctacgttc
      421 gtaaggatgc tgtcggtgaa gaaaactacg aaatcttcaa aaaagcagac cttggtgact
      481 tccttggtgt cgaaggtgaa gtgatgcgta cggatatggg agaactctct atcaaggcaa
      541 cccacatcac acacttgtct aaggctcttc gtcctcttcc tgagaaattc catggtttga
      601 cagacgttga aacaatttac cgtaaacgtt accttgactt gatttctaat cgtgaaagct
      661 ttgaacgctt tgtcactcgt tcaaaaatca tctctgaaat ccgtcgttac cttgaccaaa
      721 aaggattcct tgaagtggaa acacctgttc ttcacaacga agctggtgga gctgctgccc
      781 gtccatttat tacccaccac aatgcccaaa acattgacat ggtgcttcgt atcgcaactg
      841 agcttcactt gaaacgcctt atcgtgggtg gtatggaacg tgtctatgaa attggccgta
      901 tcttccgtaa cgaaggaatg gacgctactc ataaccctga gttcacttct atcgaagttt
      961 accaagctta tgcagacttc caagacatca tggacttgac ggaaggcatt atccaacacg
     1021 ctgctaaatc agttaagggc gatggcccag tcaactacca aggtactgaa atcaaaatta
     1081 acgaaccatt taagcgtgtt catatggtgg atgctatcag agaaattact ggtgtcgatt
     1141 tctggcaaga catgactttg gaagaagcta aaactatcgc tgctgagaag aaacttccag
     1201 ttgagaaaca ctacactgag gttggtcaca tcatcaatgc cttctttgaa gagtttgttg
     1261 aagaaacttt aatccaacca acctttgtct atggacatcc agtagctgta tctccactcg
     1321 ctaagaaaaa tcctgaagac caacgcttta ctgaccgttt cgagctcttt atcatgacta
     1381 aggagtacgg taatgccttt actgagctca acgacccaat cgaccaactt agccgttttg
     1441 aagcccaagc taaagccaaa gaacttggtg atgatgaagc gacaggaatc gactatgact
     1501 acattgaagc tcttgaatac ggtatgccac caacaggtgg tttgggaatc ggtatcgacc
     1561 gtctctgcat gctcctcact gatacaacaa ctatccgtga tgtattgctc ttcccaacaa
     1621 tgaaataaat tcttatcctc tggctcttac cagaggattt tttgattcaa aaagagactg
     1681 aatttaagga gaaaatgaag tgtagtatat tgaagttgaa atagtacact ttgatttcta
     1741 agacattgtt agaaattggt ttaaattccc taagcaattt gtgcatgttt tatttcattt
     1801 tacgatagta cgctgaaact tttcaaaaag tactagaaat tgacttggat tccccaattg
     1861 atttgttcag attcactata aataaaaaaa ttaataagtg ggataggaag ttagcgtcaa
     1921 ctaggatagt atcttgctta aacagtatat atgggactga tataagtcca taggtcctat
     1981 tagaggatgt tctggtgtct tattcacttg ttttttatag tattagtaga tagaatcagc
     2041 aaataaaaaa gtagttcaaa tgcctggcag caaaaaatct caacgattca cttaatatag
     2101 actaaggttt agagtttaag gaaagtaggg ctagtatatt gtatgaaaaa ataagatggt
     2161 ttttaagcaa aactcttgaa aatgattgaa aaaagagtta ttatagttgt atagtaaaaa
     2221 gcaaacgctt acttaatcga ggaggacagt ttatgtcata caaaacaagc aatgcagaag
     2281 gtcatgtaga cttcatcaat acctatgatt tggagccaat ggcgcaacaa gttattccta
     2341 aagcagcatt tggctatatc gctagtgggg cggaagatac tttcacttta agagagaata
     2401 tccgcgcctt taaccacaaa ctcatcgttc ctcatacact ttgcgatgtt gaaaatccaa
     2461 gtacagagat tgaatttgca ggtgaaaaac tatcttcacc aatcattatg gcacctgttg
     2521 cggcccataa attagcgaat gaacaggggg aagtggcgac tgcgcgtggt gtgcatgagt
     2581 ttggttctct ttatacaacc agttcttact ctactgttga ccttccagag atttctgaag
     2641 cccttcaagg gacaccccat tggtttcaat tttactttag taaggatgac ggtatcaacc
     2701 gccacatcat ggaccgtgtg aaggctgaag gttataaagc gattgtcttg acggcagatg
     2761 ctactgtagg ggggaatcgt gaagtggata agcgtaatgg ttttgtcttc ccagttggca
     2821 tgccgattgt tgaagaatac ctgccagaag gtgctggtaa atcaatggac tttgtttaca
     2881 aatcagctaa acaacgcttg tctccacgcg atgtagaatt tatcgctgaa tactctggtc
     2941 ttcctgtgta tgtcaaggga ccacaatgcc gtgaggacgt tgaacgttcg cttgctgcag
     3001 gagcttctgg tatctgggta accaaccacg gtggtcgtca aatcgacggt ggaccagctg
     3061 cctttgactc gcttcaagaa gtggcagaag cagttgatag acgtgtgccg attgtctttg
     3121 actctggtgt tcgtcgtggt caacacgtct ttaaagcctt ggcatcagga gcagacttgg
     3181 ttgctattgg acgccctgtc atctatggct tggctctcgg tggtagtgtc ggtgtgcgtc
     3241 aagtctttga acacttgaat gcggaattga agacagttat gcaattgtct ggaactcaga
     3301 ccattgaaga tgtcaaacac ttcaagctcc gtcacaatcc atacaaccca accttcccag
     3361 ttgaccctcg tgacttaaaa ttgtattgat aaaacagatt gcctccactg aatgtggagg
     3421 tttttcttcg tgtattggaa gaataaattg ctatcgaaaa ctatagtaaa atgaaataag
     3481 aataggacaa atc
//
LOCUS       DAVMEF010000156         3384 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVMEF010000156 DAVMEF010000000
VERSION     DAVMEF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.156"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..241
                     /locus_tag="VYQ30_001762"
     CDS             <1..241
                     /locus_tag="VYQ30_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1966830.1"
                     /translation="YDKEPYEEVGELSHRISEGGLWLDDYYLFHETLRDYRNKQSKEK
                     QEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            335..406
                     /locus_tag="VYQ30_001763"
     tRNA            335..406
                     /locus_tag="VYQ30_001763"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:368..370,aa:Glu,seq:ttc)
     gene            658..2203
                     /locus_tag="VYQ30_001764"
     rRNA            658..2203
                     /locus_tag="VYQ30_001764"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2255..2327
                     /locus_tag="VYQ30_001765"
     tRNA            2255..2327
                     /locus_tag="VYQ30_001765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2288..2290,aa:Ala,seq:tgc)
     gene            2452..>3384
                     /locus_tag="VYQ30_001766"
     rRNA            2452..>3384
                     /locus_tag="VYQ30_001766"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1001 a          663 c          971 g          749 t
ORIGIN      
        1 atacgataaa gaaccctatg aagaagtggg tgagctcagt catcgtataa gtgagggggg
       61 tctctggcta gatgattatt atctctttca tgaaacacta agagattata gaaacaaaca
      121 aagtaaagag aaacaagaag aactagaacg cgtcttaagc aatgaacgat ttcgagggcg
      181 tcaaagagta ttaagagact tacgcattgt gtttaaggag tttactatcc gtacccacta
      241 ggaagtcatg caaaaaaaat gaaaaaaatt agaaaaagta gttgacaaag tttgaaaaga
      301 ctgtataata gtaagagttg aaaataacaa ctcaggtccg ttggtcaagg ggttaagaca
      361 ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatggt atgttgcggt
      421 tggaacactt gatgaaaaaa agtttaaaaa aacttaaaaa tcttcaaaaa agtgttgaca
      481 agcgaaagca gttgtgatat actaatatag ttgtcgcttg agagaagcaa gtgacaaaga
      541 cctttgaaaa ctgaacaaga cgaaccaatg tgcagggcgc tacaacataa gttgtagtac
      601 tgaacaatga aaaaacaata aatctgtcag tgacagaaat gagtgagaac tcaaactttt
      661 aatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagta
      721 gaacgctgaa ggaggagctt gcttctctgg atgagttgcg aacgggtgag taacgcgtag
      781 gtaacctgcc tggtagcggg ggataactat tggaaacgat agctaatacc gcataagagt
      841 ggatgttgca tgacatttgc ttaaaaggtg cacttgcatc actaccagat ggacctgcgt
      901 tgtattagct agttggtggg gtaagggctc accaaggcga cgatacatag ccgacctgag
      961 agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta
     1021 gggaatcttc ggcaatggac ggaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt
     1081 ttcggatcgt aaagctctgt tgtaagagaa gaacgagtgt gagagtggaa agttcacact
     1141 gtgacggtat cttaccagaa agggacggct aactacgtgc cagcagccgc ggtaatacgt
     1201 aggtcccgag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg ttagataagt
     1261 ctgaagttaa aggctgtggc ttaaccatag taggctttgg aaactgttta acttgagtgc
     1321 aagaggggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac
     1381 cggtggcgaa agcggctctc tggcttgtaa ctgacgctga ggctcgaaag cgtggggagc
     1441 aaacaggatt agataccctg gtagtccacg ctgtaaacga tgagtgctag gtgttagacc
     1501 ctttccgggg tttagtgccg tagctaacgc attaagcact ccgcctgggg agtacgaccg
     1561 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta
     1621 attcgaagca acgcgaagaa ccttaccagg tcttgacatc cctctgaccg ctctagagat
     1681 agagctttcc ttcgggacag aggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt
     1741 gagatgttgg gttaagtccc gcaacgagcg caacccctat tgttagttgc catcatttag
     1801 ttgggcactc tagcgagact gccggtaata aaccggagga aggtggggat gacgtcaaat
     1861 catcatgccc cttatgacct gggctacaca cgtgctacaa tggctggtac aacgagtcgc
     1921 aagccggtga cggcaagcta atctcttaaa gccagtctca gttcggattg taggctgcaa
     1981 ctcgcctaca tgaagtcgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg
     2041 ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg
     2101 tgaggtaacc gtaaggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta
     2161 acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc tttctaagga taaggaactg
     2221 cgcattggtc ttgtttagtc ttgagaggtc ttgtggggcc ttagctcagc tgggagagcg
     2281 cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccattg gtgagagatc
     2341 accaagtaat gcacattgaa aattgaatat ctatatcaaa tagtaacaag aaaataaacc
     2401 gaaaacgctg tagtattaat aagagtttat gactgaaagg tcagaaaata aggttaagtt
     2461 aataagggcg cacggtggat gccttggcac taggagccga cgaaggacgt gacaaacgac
     2521 gatatgcctt gggtagctgt aagtaagcga tgatccaggg atttccgaat gggggaaccc
     2581 aacaggtagt acctgttacc cacatctgtt aaggatgtga ggaggaagac gcagtgaact
     2641 gaaacatcta agtagctgca ggaagagaaa gcaaaagcga ttgccttagt agcggcgagc
     2701 gaaacggcag aagggcaaac cgaagagttt actcttcggg gttgtaggac tgcaatgtgg
     2761 actcaaagat tatagaagaa tgatttggga agatcagcca aagagagtaa tagcctcgta
     2821 ttttaaatag tctttgtacc tagcagtatc ctgagtacgg cgggacacgt gaaatcccgt
     2881 cggaatctgg gaggaccatc tcccaaccct aaatactccc tagtgaccga tagtgaacca
     2941 gtaccgtgag ggaaaggtga aaagcacccc gggaggggag tgaaatagaa cctgaaaccg
     3001 tgtgcctaca acaagttcga gcccgttaat gggtgagagc gtgccttttg tagaatgaac
     3061 cggcgagtta cgttatgatg cgaggttaag ttgaagagac ggagccgtag ggaaaccgag
     3121 tctgaatagg gcgccttagt atcatgacgt agacccgaaa ccatgtgacc tacccatgag
     3181 caggttgaag gtgcggtaag acgcactgga ggaccgaacc agggcacgtt gaaaagtgct
     3241 tggatgactt gtgggtagcg gagaaattcc aaacgaactt ggagatagct ggttctctcc
     3301 gaaatagctt tagggctagc gtcgacatta gagattcttg gaggtagagc actgtttggg
     3361 tgaggggtcc atcccggatt acca
//
LOCUS       DAVMEF010000157         3382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVMEF010000157 DAVMEF010000000
VERSION     DAVMEF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.157"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(95..1645)
                     /locus_tag="VYQ30_001767"
     CDS             complement(95..1645)
                     /locus_tag="VYQ30_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1966831.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1661..2440)
                     /locus_tag="VYQ30_001768"
     CDS             complement(1661..2440)
                     /locus_tag="VYQ30_001768"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1966832.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2427..3278)
                     /gene="ylqF"
                     /locus_tag="VYQ30_001769"
     CDS             complement(2427..3278)
                     /gene="ylqF"
                     /locus_tag="VYQ30_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1966833.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LIKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
BASE COUNT          990 a          711 c          671 g         1010 t
ORIGIN      
        1 ccgatctcat ttctgagaat cggttttcta aataaatcaa gccgaataaa tcaagccgat
       61 catttacata acataaatta tgtaaatgat cgtactacaa caacaaatct ttgacttttc
      121 caatttcact ttttggaata accaggtgaa tcatatcacc cagatacatt ctggttgagc
      181 cgttaactgt ttggctcttg ccattatgga cttgagttgt gatgaggacg ttgtgtggta
      241 agttgagttc atgaacttgt ttcccagcaa ttttatcaga aactggtatt tcgataagtg
      301 taacttctcc ttcgctagat acttcttctg gaagcatttt ttccagcatg gcttcataga
      361 ctggcgtacc tttgagcaaa tccatgataa tataagaaac aagagtgaca agaccaagtg
      421 gcatgaggtt gcgaatatct cctaccatct cagttacgag gatcatagca gttaagggag
      481 cttttgatat ggctccaaaa tagccactca ttcctagaat gacaaatata gggaattgct
      541 cttgactgac aagtccaaga ttgacacaga taacaccaac taaggcacca agcaaagaac
      601 caagagctaa aatggggagg aaaattcctc ctggcagtcc acttccatag ctaatcatac
      661 tccaaataaa gcggattaaa aagtaagcta ataaaacttg gaaactaaaa ttttgttcag
      721 ttaaagaaag gacaagctga tttccgccac caatgatttg aggtaagaag attccgactg
      781 gtatgataag gataaaagcc aagatgggat aataagccct atccaaatgg attttttgac
      841 caatcaagtc ataaactctt ccaacgttta atacagcttt ctcatagaga aaacctgaaa
      901 atccaaggaa aattcccatg acgagatata tccaatactg gtctagggtc atgggaggaa
      961 tgttatctgg catatccaat actggtgtca aaccgaacat gagtagagac acaaagtttg
     1021 ctacgatgct ggctgctaga gttgagaccc agaaaaagcg cgaaaaatgg tgatagactt
     1081 cttctacaac aaagagaagt gctgcaatag gagcattaaa ggctgcggct aaacctgctg
     1141 cagctccact ggcaatcaag gaacgttcct ctactggact ggatttgagc cacttggcaa
     1201 ttcctttacc accaactgct ccaagttgaa tgctgggtcc ctctcgaccc agcatgagtc
     1261 cactggcaat agcaagaata cctagcacat attttttcca aagaatgccc caccagttga
     1321 gggacatgag gcctttcagt tcggcttcga cttgaggaat tcctgagcct ttaatatctt
     1381 tttctgaccg tgttagtttg gcactgagcc aacagatgag tatataaaac aaaaccagta
     1441 caaaaagatt gcgcactaag tacccttgat cttgataaac tccttgtatc aggtggaagc
     1501 ccttttcaat taagaaacga aaggatccga cgataattcc aacgatgagg ccgacaatga
     1561 ttcctcgacc aacttgggat agtatagtgc tggatgcaaa ggcgaattct ttcttagaac
     1621 ggactatttc cgactgttcc tccatggtat ttcctttcaa ttaactttct tttttaccta
     1681 aaaccagtga tttaacgggt tcaaagctgg ttcggtgaat tggggtaact cctagttttg
     1741 tgaggccttc cagatgttta gctgtgccat atcctgcatt agtagcgaaa tcatagccag
     1801 agaactgctg atcgtattct ttcatcaatt catcacgtgt taccttggct actatagatg
     1861 ctgctgcgat agagagggag ttggcatctc ctttgataat ggaggtttgt gaaatgggca
     1921 agtccagttt catggcatca atcaaaaggt gctctggttg agggctgagc tgggagattg
     1981 cttcttgcat ggctagtttg gttgcttcat agatgttgac ttggtcgatg acctgattat
     2041 ctatgatacc aattccaatc gacaaggctt ggtcttgaac ggcttggaaa atctccagat
     2101 gtttcttttt aggaattttc ttgctgtcgt tgagaccttt aatcttacaa tttttaggta
     2161 aaataacggc cgcagcgact acaggaccag caagaggacc acgaccaacc tcatcaatac
     2221 ctgcaattaa ggtcaatcct tgcttataaa gttctttttc ataagaaagc atggattcca
     2281 agcgcaaatt ttcatctaat tcagcttgaa tggctctttt acgcttgctg atttcctttt
     2341 gaactcctga gcgattatcc ttttcaagct ctaaaaaaat agggctttct aactccttga
     2401 ctgtcacaag gagttctttg atttctttaa tcgttgccat cgaggtcttc caatgtatct
     2461 aaggtatagt taccgagttt gccatcacgg acttccttca cgaagagact gtaaaaacgg
     2521 tcatagtcat cacggaaacc gagggcgcgg gtcatatcca taataatcac aggcgcttct
     2581 tcttcaattt tcatttgttt gaagcgttca gccagctttt ctggataatg ttctttgaaa
     2641 taattgatac caaaaatggt aacctcatcc ataggaagca actgatcctt gatagctcca
     2701 gtcaatgcca acttaagtgc aacagtttca tcctcaaact taggccagag aatccccggt
     2761 gtatccaaga tttccagatc tttattggtt ttaagccatt gttgaccttt tgtgacccct
     2821 ggcttgtttc caacaacagc aatcttttta ccagccaaac ggttcatcag agttgattta
     2881 ccagcgtttg gaatcccaat aatcatggta cgcaaggttt caatctgaat cccacgttct
     2941 ttctggcgag caatcttatc agccatgagc ttcttggccg catctgttac aacttttaca
     3001 gtcacttgct ctttggagtt gatagctagc gtctggattc cttgtgattc aaaatactga
     3061 cgccattcct tggtcattgc tggatcagcc aagtcggcct tgtttaaaat caagagtttt
     3121 ggtttatcac caacaatctt gatcaacata ggattttgac tagatagagg taagcgtgca
     3181 tcaactaaaa tcgtcacaaa atcaacaaat tttaaatttt cctgcacctg tcgacgcgct
     3241 ttagacatgt gaccaggaaa ccattgaata gtagccattg agttttttcc tttcgtagca
     3301 aggatttaga tcccctattt tattttacta tagtttgaac atagagagtt ttcaaatgaa
     3361 tgatgtccaa acagttcttg tt
//
LOCUS       DAVMEF010000158         3317 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVMEF010000158 DAVMEF010000000
VERSION     DAVMEF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.158"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..88)
                     /locus_tag="VYQ30_001770"
     CDS             complement(<1..88)
                     /locus_tag="VYQ30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1966834.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQ"
     gene            complement(265..876)
                     /gene="rpsD"
                     /locus_tag="VYQ30_001771"
     CDS             complement(265..876)
                     /gene="rpsD"
                     /locus_tag="VYQ30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1966835.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1071..2123)
                     /locus_tag="VYQ30_001772"
     CDS             complement(1071..2123)
                     /locus_tag="VYQ30_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1966836.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2120..2818)
                     /locus_tag="VYQ30_001773"
     CDS             complement(2120..2818)
                     /locus_tag="VYQ30_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966837.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2985..>3317)
                     /locus_tag="VYQ30_001774"
     CDS             complement(2985..>3317)
                     /locus_tag="VYQ30_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1966838.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMSADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          930 a          743 c          634 g         1010 t
ORIGIN      
        1 tttggaaaac gtgtgatgct tctgttatac tacctgttcg ctcacaataa gagagaactt
       61 ttttacgaaa atctattgaa tatgccataa aagattatac cacattgtgt actatttttg
      121 gttcatttta ctatatcact ttggactata tcggtctagt tatgtctaaa cgattatagc
      181 tggatactaa gagaaatcaa aaaagcacta agggagaacg tcccaaagtg cttaatatca
      241 aagcctatct tattcaataa aatattacaa cattttgttg tagaattcaa cgacaagcgc
      301 ttcgttgatt tctgggttga tttcgtcgcg ttctggcaag cgagtcaatg aaccttccaa
      361 tttttcagcg tcgaatgata cgaatgctgg acgtccaaga gtagcttcta ctgcttcaag
      421 gattgctgga actttcaatg atttttcacg aactgagatc acttgacctg gagttacgcg
      481 gtatgatggg atatcaacgc gtttcccgtc aacaaggatg tgaccgtggt ttacgaattg
      541 acgagcttga cgacgagtag tcgcgagacc aagacggtaa acaacgttat ccaaacgacg
      601 ttccaaaaga agcataaagt tgaaacctag gattccgcct ttgatttttg tagcttgtac
      661 gaacaagtta cggaattgtt tttcacctac accgtaagtg aaacgaagtt tttgtttttc
      721 agccaattgc aaaccgtatt ctgacaattt agaacggttg tttggtccgt gttgtcctgg
      781 tacgtagtta cgacgtgcca attctttacc tgtacctgta agtgaaaggc caaggcgacg
      841 agcttgtttc caagatggtc ctgtataacg tgacatatga atgtcctcct gatataaata
      901 atatttcggt ggaaatagtc acttagaaag ccctgattcg tgcagatgcc cttcgcctaa
      961 acagccaagg ttacttatca taagacacct gttgacgagc ttcatgcttt cctgctgata
     1021 tttcacacag agtccatttt accatgaata gctggatttg taaaggggtt ttaggcttta
     1081 ttttcactac cagagaggtt gagaacgagg gtaaaggtac ttcctagacc gtactggctg
     1141 ctgactgtga tttccccacc caattgatgg gccaattcac gcgcaatcgc aagtcctaat
     1201 ccatgaccac ctgtcttcat gttacgcgaa gtttcgacac gataaaggcg tttgaaaata
     1261 ttttccaaat cctctggggc aatgccctgc ccttcatcgg tcacactgat tgaaagctgg
     1321 cccttctcca gcttagccac cacttccagc ttggttcctg gagcagaata tttaaaagcg
     1381 ttatcgacca gattcaccaa gatacgagaa agcttagcat aatctccctc aatccgggca
     1441 gactctggga ttacctgcaa gtggacatct cttctctcct gctcaatcaa aaactgaaat
     1501 tcactcatgc actcaattaa gagcttgtcc agaaaaatac tgtctttact ggtagtttcc
     1561 acctgatttc tagctgtgtt tagggtcaaa aaattcaact cctcaaccag tttattgagc
     1621 ctctccgtct ggcgtccaat ggttgctaga taatgagctt gctccgactc cttgataatc
     1681 ccatccaaaa tcccttctac cgtcgcttgg atcgaagtga taggagtctt aatatcatgc
     1741 gacaactggg caatcatcaa gcccttttct cgttcgcttt cttccaagga atcaaagctt
     1801 acctgcaaat catgggacat ctcattaaaa gtttgcccta attgctgaaa ttctacagga
     1861 ccttgaacct ccaaatttga aggaaaatcc ttggccgcta cccgcttggc atgctccttg
     1921 agtttgccca acgacgtaaa gactggcaat aggagaaaga gactaatccc agcaccgaca
     1981 aggctggcaa cgatagtcat cccaagcaaa aagtaaatct cgcctttcgc aatcagcatt
     2041 ttttgaacag cccaaaaaac aaccaaaatg gttaagaggg ttgaaataat atatccaacc
     2101 aaaatataac tttttagttt catgtttgtc ctctcggttt ctctatctta tatcccaacc
     2161 cccaaactgt cttaatagtg ggcgtttggt cactactata ttttgccagc tcctgtcgaa
     2221 gagcatggat atgcacattc aaggtattgg tgtcatccac gtagtcttct ttccagatct
     2281 tttcatagag gtctgtcttg gagaaaactc gctctggatt gctagctaaa atccacagca
     2341 attcaaatga tttaacagtt aaatccagca tttcttctcc tatttgaact tcatgactac
     2401 tatgattcat ttttagattg ccaagggaaa tcagctctgt ttcgccccca cgatgaaggc
     2461 ggcgcaaaat attgtggaca cgcaaaacta gctcacgtgg gctaaaaggc ttagcaataa
     2521 aatcatctgc tcccaagctc aggccgtaaa tcttgtcctg ttcactggtc ttagcagtaa
     2581 taaataggaa aggctgctct ggtgataagt attgaacctc actgattaaa tcataaccat
     2641 ccatccgagg catcatgaca tctgtgataa tcaaatcaat cggttttttc ttgaacagct
     2701 ctagcgcttc cagtccatca tgggctacca agacctgata acctgcctga attaagtatc
     2761 tctgatgaat atctgtgatt tctacctcgt cgtcaacgag taaaattgtc tttcccatct
     2821 gtctctcctt tgataaaaac agtgctatac tgctttaagt ataacactat ttttattgtt
     2881 ttacgatgat tttaatctgc tattgaaaga aagtcctgat tgctgattga gttgttaaac
     2941 taatcttagt cagcccacta tccatcattt atttccttag ctgattagtt ttcttctttt
     3001 ttgtttttca aacctagacc accgagtaaa cctgcaagcg caagcccaag gaaaccaata
     3061 cttgccattg atgtttgagt ctcaccagta tttggtagca tagctttatc ctctgacatc
     3121 atcgtatcag acatcttgtt agcagaagca gccatgtttt cacctgccat cgtgttggta
     3181 gaacttgtca tggtgtcagc agacatgcta tctgtagtac ctgtagcatg attgtgattc
     3241 atcggagtca cgccagaacc agagttagaa ggtgataatg aaccatttgc tgtgtctgat
     3301 gtttctttaa catttat
//
LOCUS       DAVMEF010000159         3284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVMEF010000159 DAVMEF010000000
VERSION     DAVMEF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.159"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..2293
                     /locus_tag="VYQ30_001775"
     CDS             20..2293
                     /locus_tag="VYQ30_001775"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1966839.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETVFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2310..2780
                     /locus_tag="VYQ30_001776"
     CDS             2310..2780
                     /locus_tag="VYQ30_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1966840.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3023..3124)
                     /locus_tag="VYQ30_001777"
                     /pseudo
     CDS             complement(3023..3124)
                     /locus_tag="VYQ30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT          999 a          735 c          587 g          963 t
ORIGIN      
        1 cattctatat ggaggttcta tgcgttttaa tcaatatagt tatatcaatt ttccaaaaga
       61 aaatgtctta tctgaactaa aaaaatgtgg ctttgattta caaaatacag ccaatcacaa
      121 agactcactt gaaacctttt tacgtcgttt ctttttcact tatcaagaca ccaactaccc
      181 actttctatc ctagcagctg ataaaaaaac agacctgctg acattttttc aatcagaaga
      241 tgaactgaca gcagatattt tttatactgt tgcttttcaa cttttaggct tttcttattt
      301 ggttgatttc gaagatagtg acgtttttcg taaagaaact gtatttccca ttatatatgg
      361 tgacttgatt gaaaatctct atcagttact caatactcgc accaaaaaag gaaatactct
      421 tatcgaccaa cttgttagtg atggtcttat tccggaggat aatgactacc actactttaa
      481 cggcaagagt ttggctactt tttctaacca ggatgtcatt cgtgaagtcg tttacgttga
      541 gtctcgtgtc gatactgacc aaaaagggct atcagaccta gtaaaggtta gcattattcg
      601 ccctcgtttt gatggaaaaa tccctgccat catgacagcc agcccttatc atcagggaac
      661 caatgacaag gctagtgaca aggctctcta caagatggag ggcgagcttg aggttaagct
      721 tcctcacaag attgagctag agaaacctca actaaatctc gtccaacccc aaggtaaagc
      781 tgagcttata gcagaggctg aggaaaagct aactcacatc aactctagct atacactaaa
      841 cgactacttc cttccgagag gctttgccaa tctctatgtc tcaggtgttg gtaccaaaga
      901 ctcgacaggt ttcatgacta atggagacta ccagcaaatc gaggcttata aaaatgtcat
      961 cgattggctt aacggccgtt gccgtgcctt tactgatcac acgcgccagc gtcaagtcaa
     1021 ggctgattgg tcaaacggaa aagttgctac aacaggactt tcctatctag gtaccatgtc
     1081 caatggtctt gcgactacag gagtcgatgg tttagaagtt atcattgccg aggcaggaat
     1141 ttcatcatgg tacaactact accgtgaaaa tggtctggtg actagcccag gtggttatcc
     1201 aggtgaggac tttgactccc ttgctgagtt aacctattct cgtaatctct tagctggcga
     1261 ctatatccgt ggcaatgaag ctcaccaagc tgacttagaa aaagtgaaag ctcagctaga
     1321 ccgaaaaact ggggactaca atcagttttg gcatgaccgc aattatctgc tcaatgccca
     1381 taaagtaaag gcagaggttg tctttactca tggttctcag gattggaatg tcaaaccact
     1441 tcatgtttac cagatgttcc atgctcttcc tactcatata cacaagcatc tctttttcca
     1501 taatggtgct catgtttaca tgaacaattg gcaatcaatt gacttccgtg agtccataaa
     1561 tgccttattg accaagaaat tactaggaca ggaaacagac tttcaacttc ctactgttat
     1621 ctggcaggac aatacagctc cacagacttg gttatcactt gataacttcg gtgggcaaga
     1681 aaactgtgaa accttctcac ttggtcaaga agagcaagct attcaaaacc agtacccaga
     1741 taaggatttt gagcgctatg gtaagacata ccaaaccttc aatacagagc tctatcaagg
     1801 gaaagccaat cagattacta ttaaccttcc tgtgactaaa gatctccacc taaacggtcg
     1861 cgctcagctc aatcttcgta tcaaatccag tacaaacaag gggctcttat ctgcccaact
     1921 gctagaattt gggcaaaaga aatacctaca accttatcca gctattttaa gtgctagaac
     1981 cattgataac ggtcgctatc acatgttgga aaatctctgt gaattgccat ttagaccaga
     2041 ggcacaacga gtcgtaacaa aaggttacct taatttacaa aatagaaatg atttactgtt
     2101 agtagaggat attactgcag atgaatggat ggacgttcaa tttgaactgc agccaactat
     2161 ttacaagcta aaagaaggag acactctccg tttagtcctc tatactactg actttgaaat
     2221 caccatccgt gacaatacag actaccactt gactgtcgac ctcgctcagt ccatgcttac
     2281 cttaccttgc taaaaggagt tacattatta tgaataaatc agaacaccgc caccaactta
     2341 tacgcgctct tatcacaaaa aacaagattc atacacaggc tgagttgcaa gcccttcttg
     2401 ctgagaacga cattcaagta acccaggcaa ccctctcacg cgacatcaaa aatatgaacc
     2461 tatcaaaagt ccgcgaagaa gatagcgctt attatgttct taacaatggt tccatctcaa
     2521 aatgggaaaa acgtctcgaa ctctacatgg aagacgccct tgtctggatg cgcccagttc
     2581 aacaccaagt cctactaaaa acccttcctg gactggctca atcctttggt tctatcattg
     2641 atactttgag cttccctgac gctatcgcta ccctttgtgg taatgatgtc tgtcttatca
     2701 tctgtgaaga tgcagatact gctcaaaagt gctttgaaga actgaaaaaa ttcgccccac
     2761 catttttctt tgaagaataa taaatgacta caactaacaa ttatgctttt aaaaaacctg
     2821 gtagaaagtc tttaacatca gaaaacgcat agtatcaagt gttgaaaacc cttgatacta
     2881 tgcgttttgc tttatcctag gaagatttat actcttcgaa aatctcttca atccgcgtca
     2941 acgtcgcctt gccgtaggta tggttactga cttcgtcagt ttcatctaca acctcaaaac
     3001 catgttttga gctgacttcg tcagtcttat ctacaacctc aaaacagtgt tttgagcagc
     3061 ctgcagctag tttcctagtt tgctctttga ttttcattga gtattatttc attttctcct
     3121 gcaattgaat tcttgctcgg ctttttgttt tctatttctt taaaatcaaa gtagctcttt
     3181 tgttaataac tcgatcaaca aacatcgtgg tacaagtatc tactttgaaa tttatcaacc
     3241 acttaacaac tgatactgta ttctaggaaa acgatgacat tctt
//
LOCUS       DAVMEF010000160         3267 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVMEF010000160 DAVMEF010000000
VERSION     DAVMEF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.160"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            280..2870
                     /locus_tag="VYQ30_001778"
                     /pseudo
     CDS             280..2870
                     /locus_tag="VYQ30_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            3170..>3267
                     /locus_tag="VYQ30_001779"
     CDS             3170..>3267
                     /locus_tag="VYQ30_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HEV1966841.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFG"
BASE COUNT         1002 a          482 c          731 g         1052 t
ORIGIN      
        1 tcttatggga ctttttttct acccaaaata ggctccataa tatccatagg gaatttaccc
       61 actacaaata ttatagagcc caaagtttta ggtcgcttga taatatgcgt tttttgaatt
      121 ttatagactg ctcgtttaaa ctctatttac ctcgtacatt ccggagcgag atggactatt
      181 ggtcgcatac aaaatgagtg ttattcggat tttattttcg tgtacaattt cagccattct
      241 tgttataatc agcctatagg aatcaaggag gtgattgtta tggctgtttt tgtgtctttg
      301 gatggaattg tggtagaagt acttgatgtc ttttcttctt ttaatgggga tagtgagttt
      361 ttcttgtgta aacgtcttaa agacaagagt cagtttgtga tggaacgctc tcggttcgag
      421 gagatgttcc aacttcaaag tagtcacttg acgacgcaag aaaaattaca attgtttacc
      481 tccgtgtttg ctggccgtta tgatgtttat gctaagagtt ttatcaatga tcaagggaaa
      541 attcagtatt ttccttccta tgattatggt tggaagcagt tgccacctga aaaacggagt
      601 ttccagacat tgacggactc cgttttgaaa tctcattttc gtggggagac agctatcggt
      661 atctttccta tgcacttaga tgatagctgt tattttttgg tactggattt tgatgaagga
      721 gattggaaag aagccggttt agccattcga agaatagcca gggaacgcca gatggaagcc
      781 catctagaga tttctcgttc gggtcacgga ctccatattt ggttcttctt tgaggaagcg
      841 attttgtgtc gagaggctcg cttatttgga aagaaactat tagaattggc tatgcaggaa
      901 agtatgcaac tgtcctttga ttcttttgat cgcatgtttc caaatcagga tgttcttcct
      961 aaaggtggat ttggtaattt gattgccctt ccttttcaag gagaagctta tcatcaaggg
     1021 cgaacggtct ttgtggatga acagtttcag ccttatgaag accaatggag gtatctacaa
     1081 gagattcaga gggtttcaac tgctaaagtg gcactgttaa tccaagagga gttaggcaag
     1141 caagaattgg ataaggagtt gaaggtcgtc ttatccaata tgatccaact tgaaaaatcg
     1201 tctgtgacac ccaagacact ttttttcttg aaaaatatgg cttcgttttc taatcccgaa
     1261 ttttatttaa agcaggctat gcgacagcca acctatcaaa ttcctgagag aatgtattta
     1321 tttggagaat ccgattatta tttatggctg ccaagaggct tgttatatcc attgcaagat
     1381 aaatttaagc aggtagttgt ggaggatagg agaaaagtac aaaggtctat tagagtagca
     1441 tttaagggag agcttacttt ggagcaagaa ttagccctgt cagatatgaa ttctaaagaa
     1501 aatggtttac ttcatgcggg acaggttttg gaaagagtgt tttaggtgct gctcttatct
     1561 ctgaacgtaa gacaaaaacc attatcctag ttcataatag gcaactctta gaccaatgac
     1621 tggatcgttt aaagtgtttt ttgactttcg aagaggcaga agctacccgt tatacaccat
     1681 caggtcgtga aagggtaata ggctatgtcg ggcagtacgg tggggctaag aaatgactga
     1741 gtaaactggt tgatgttgtt atgattcaat ctctatttaa gttggaaaat agtcaaagtc
     1801 ttttggatga gtatgagatg atgattgtgg atgagtgtca tcatatctct gccttgatgt
     1861 ttgaaaaagt tgttgctcag tttagaggga agtatcttta cggtttgacg gctacgcctg
     1921 agcgtaagaa tggtcatgaa cctattgttt ttcagagaaa ttgaaaagac caaagcaagt
     1981 aattttatac agcccagtga ttggattgct acagactcag cgaggaatca gttgattctc
     2041 aaggatattc tagcccaagt ggcagaagga cggaatatct tgggtttagt taatcgaatt
     2101 caacagatag atgtctttga aaagttattg aaagagaaag aggttgatga ctgttacatt
     2161 attagcggaa aaaccaacgt ccgagaaaga gaacgagttt actggagacg ttagaacagt
     2221 tagataaagg gtttattttg ttgtttactg gaaaatacat tggcgaaggt tttgacttac
     2281 ctcagttgga cacgcttatc ttggcagcac ccttttcttg gaaaaataat ttgattcagt
     2341 acgcaggtcg gattcataga aactacaagg ataagtcttt ggtgcgtatt ttcgattatg
     2401 tggatattca tgttccttat ttagaaaaga tgtttcagaa acgacaagta gcttatcgaa
     2461 agatggatta tcgtgccatt gagggtgagg agaaacaatt cgtttatgtt gatagtagat
     2521 atgagaaggt gttgagagag gacttagcag gggaaagaca ggaatgtatg cttattttac
     2581 cttatgtgca ccagacaaaa ctgatgaatt ttctaaaaga atttaggatt agtcaaattg
     2641 agatatgtat accagagacg gttgcaaata aagcatggct agaccagttg aagagccaga
     2701 aaattaaagt gttttttact caatcaaaaa tagtaacgcc tattcttttg gtgaataaga
     2761 ctattgtttg gtatggtgca atgccattat tagggaaggt agatgagatg accatattac
     2821 gtttggaatc agctagtata gtttctgaac tagtggcagg tttacgatag agaaaatttt
     2881 taaaaatttc tatgtatgat tttcatttct ttagtgagac tgttgccatt atcacattcg
     2941 aatcacacaa aataaaaaaa tttctacaag tacttgacaa atagattgaa atatcttaaa
     3001 atgaaaatgg ttacagagtt attaattatt taagcttcat gtcaccatta aaaattgaaa
     3061 taaaaggatg ttatcactaa tacaagtgag caggaaccta tttaatcaca tcggaagaag
     3121 tttcttgatg tttttaagta ggttcctttt attttaaaag ggaaatttta tgatcataaa
     3181 acgaatacta aaccacaatg ccgtaattgc gcaaagtaaa aaagatatcg atattcttct
     3241 ttttggaagg ggaatagctt ttggaag
//
LOCUS       DAVMEF010000161         3255 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVMEF010000161 DAVMEF010000000
VERSION     DAVMEF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.161"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..298
                     /locus_tag="VYQ30_001780"
                     /pseudo
     CDS             1..298
                     /locus_tag="VYQ30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            371..817
                     /locus_tag="VYQ30_001781"
     CDS             371..817
                     /locus_tag="VYQ30_001781"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966842.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERISIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            1157..1411
                     /locus_tag="VYQ30_001782"
     CDS             1157..1411
                     /locus_tag="VYQ30_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966843.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            1522..2628
                     /locus_tag="VYQ30_001783"
     CDS             1522..2628
                     /locus_tag="VYQ30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1966844.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPTTVTVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKENRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            2586..3107
                     /locus_tag="VYQ30_001784"
     CDS             2586..3107
                     /locus_tag="VYQ30_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1966845.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYATGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
BASE COUNT         1198 a          555 c          679 g          823 t
ORIGIN      
        1 gagcattaga ttacatcaaa gaggggcaca gccatgtcga ggcagccaag ttttttggtg
       61 ttggcgtcag aactctcttc acgtgggaaa agaaagacgt gaacaagaac acatagagag
      121 gaaaaagcga gtcgtcaaaa accgaaagat tcctttagag gaattgaaag cctttgtaga
      181 ggctcatcca gatgcttttt tacgggaaat tgcggcacat tttgattgtg ctgttccttc
      241 agtatgggca gctttaaagc agattaaggt cactttaaaa aaagatgacg agctttaagg
      301 aacaagaccc agaaaagtag ccttatttct taagaatttt aatagtttaa agcacctagc
      361 acctgtttat attgatgaaa caggaatcga ccgctatctc tatcgtcctt atgcaggggc
      421 tcctagaggg gagaaagtct atgaaaagat tagcggacgt cgttttgagc gaatttcaat
      481 tgttgcagga caagtagacg gagagtttat agctcccatg atttacaaga aaagcatgac
      541 aagcgatttc tttgtggagt ggttcaaaac gcaactccta cctgctttga agacacctca
      601 tgttattgtc atgggcaatg ctggttttca tcccaagaac attttggatg aactctgcat
      661 ccaagataaa cactttttct tacctctacc accttattca ccggatttga atcctattga
      721 gcaagcttgg gctatcttga aaaagaaagt gacggattta ttaagggaag ttccaactat
      781 ttttgaatgt ttggaatgct tttttaaaac tagatgacta taacggttct aaaggaacct
      841 atcgagtagt cattaaaact aaggatactg ctggttaaga gaagacggta tacaatcaaa
      901 ccattcaccg tgtagccgaa atcgttcaga atgaagactt gtataaaaaa ggtttgaatg
      961 ttgaacttgc gcaccaacaa attaagggat tttttgaagc agagtttaaa aatcgtatta
     1021 atggagttct taatactaaa ataaaaaata gtacattaaa tcgtgtaaat aaaaaaacta
     1081 tacaccagag caacaaaaac tccatgatca atttgaagca gaagcaacgg aagatgctaa
     1141 aaaacaaggc gatattgtgt tgaatgttga ccaggatttc atgagcatat ctaagtctaa
     1201 taaaagtggt tcagactgga agaaaacttt cacagtgagg acaaccaata ggctagcaaa
     1261 tgacttgaat aatgtcttga aacaggctga taaagatact cctaataccc caacttggct
     1321 aaactcagct gcttctaaag ctaaagatga tgacagagca tataaactac tgaagactct
     1381 tataccagga gaaaattacc tatcatgtta aggataatca gctagaagta gaaacagata
     1441 aatacacata tactgccgct agaaatggta gtaaggaagt tggtattcaa gagtcagata
     1501 tagcagcaac tctaagtgcc gatgaatata attctaatcg ccaaactttt gagagagaat
     1561 acaaatacaa aagcaaatgc ccttaataat ggttgggcta gatctggttc tgaagagttc
     1621 aaaaagttct cccactttgt aggggtagac aaagggattg tgcgaacgaa tgtactgact
     1681 ggtaaaaaac tatctgataa gattaggaaa gaagtgggct ctggagatag caaactagga
     1741 aaaggcggct atttctctac tggggatgtt ctattaggaa aagatgttgt ttcttatacc
     1801 gtacaagtat tttcagagaa taatgaaaga gtaggagtaa acactcaaag tcaccgtgtt
     1861 cagtataatc tcccaattct agctgacttt tcagtcatcc aagatactgt ggaaccatca
     1921 cgaaccgttg ttgaaaaaat cattccaaaa ctaaatattc ccgaagaaga gaaagggaaa
     1981 ataaccgaag aaatcaagaa aaagaaaaaa acctcagaat tggcagaact aatctcagaa
     2041 aatgtgaaag ttcgctatgt tgatgaacaa gggcgtttgc tatcattgaa aaatgatact
     2101 ggaattggag aaaaagaaag tgacggaacc tacattacca ataaaaaaca actgattggt
     2161 accagctaca atgtcacaga taaaaaactc agtagcatga ctactactga cggaaaatat
     2221 tatactttta aagaagtaga tacaaattct gcaagtttaa ctgggaatat tgtaagcgaa
     2281 ggtagaacag tgaccttagt ttatagagaa agcgaagcgc caaccactgt tacagtaaca
     2341 gccaattact ataaagaagg tagccaagag aagttggcag agtctgttat aaaagctgat
     2401 ttagcgatag gttctgagta taccacagaa tcaaaaacta ttgaagggaa aacaacaact
     2461 gaggacaaag aaaaccgagt tatcacaagg aaaacaacat acaccttggt agcaactcct
     2521 gcaaatgcgt accagaagac ggtgcaacag ttgactatta ctaccgttag aatgttgagg
     2581 aaacagtggt tcccaaaaca gcaacctcta ctgagacgaa gactataacg cgtatcattc
     2641 attacgttga taaagttacg aaccaaaatg taaaagaaga tgttgttcaa cctgtaacct
     2701 taagccgtac aaaaactgag aacaaggtca cgggagttgt aacctatggt gaatggacaa
     2761 caggaaactg ggacgaggtt atatctggta agattgacaa gtacaaagat ccagatattc
     2821 caacagttga atcacaagaa gttacgtcag actctagtga taaagaaata acggtaaggt
     2881 atgaccgttt atcaacacca gataaaccaa ctccagaacc aggtactcca aaaactgaaa
     2941 ctccagtgaa tccagaccca gaagttccga cttatgcgac aggtaagaga gaggaattgc
     3001 caaacacagg tacagaagct aatgctacct tggctagtgc tggtatcatg accttgttag
     3061 ctggtctagg attaggattc ttcaagaaaa aagaagatga aaaataatag attttagaat
     3121 ctaggaacca ggaaaagctc atagatgtgg gctttttcct ggttttgaga atgaggtctt
     3181 tcgtgaagaa taaaaacgct tacaagtacg ttgaactggg aaactatgaa tcctattttt
     3241 ttaaaaatat ttcca
//
LOCUS       DAVMEF010000162         3245 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVMEF010000162 DAVMEF010000000
VERSION     DAVMEF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.162"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(6..1451)
                     /locus_tag="VYQ30_001785"
     CDS             complement(6..1451)
                     /locus_tag="VYQ30_001785"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1966846.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKILAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISQDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQNHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            1557..3179
                     /locus_tag="VYQ30_001786"
     CDS             1557..3179
                     /locus_tag="VYQ30_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1966847.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVSKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLAMSLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT          816 a          661 c          726 g         1042 t
ORIGIN      
        1 tattcttata aataattttc tgcgacggct gtatctcctg ggtaggattc tttcttgccc
       61 tggatgattt ggtaacaatc ggctccctta cccgcaataa taactgcatc taattcgtga
      121 tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt ttcaacagga
      181 tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata gttagggtca
      241 tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc aaagtcctta
      301 cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt tccggtttga
      361 tgagtttcaa ccacattgat gagttttttc agactatccc cattgtgggc atagtcgatg
      421 aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac gcgggttgca
      481 gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc aagtccagca
      541 gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc tccagcgagt
      601 ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc aaattggcta
      661 ccatagaaat catggttttg atcttcaacc tgttctttca agactgagaa gtggtccatg
      721 tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg gtagaaatag
      781 tcttcaaagc tagggtgttc aatcgggccg atatggtctt ggctgatatt taggaaaact
      841 cccacatcaa aggttagacc atagacacgt ttgaccagat aggcttgact ggagacttcc
      901 atgatgaggt gggtacggtc attttgcaca gcctgattca tcatgtcaaa gaggtcaata
      961 ctctcagggg ttgtcaacgc tgacttaaag aaagtctcgc catcaagagt tgtgttcatg
     1021 gtcgacaaca tagcaggtcg atgcccttga gataagatgt tataggcgaa ataggctgcc
     1081 gttgtcttac ccttagttcc tgtgaaggcg agaattttga gtttctcttg tggattacca
     1141 tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat gatgacaggg
     1201 ataccgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt tatagcagaa
     1261 agaaggtatt cttttttaaa ggcagcgcct tttgcaaaaa aaagagtgtc ttctgttact
     1321 tttcggctgt cgtagctgat gctatcaaaa acaactttgc tgtagttgta gtggtaatga
     1381 ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa tacggtttca
     1441 atcttaatca tactttctat tgtaaaccga aagtcgcaaa tttacaagta acaaggaaaa
     1501 gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag gaatctatgt
     1561 cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg ctaacggcta
     1621 gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac atctggatgg
     1681 gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc tatgcttggt
     1741 tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt tccaagtata
     1801 ataccatgcg agaagaagag catagctttg ccctgattcg gagcttctta ggctttatga
     1861 caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg ctagcagact
     1921 tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg ggagtcttga
     1981 ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac ctcaaaccct
     2041 atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc ctagcaacct
     2101 ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa tcaacctttg
     2161 ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt gcccaagaag
     2221 gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt ctcttggttg
     2281 ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag ctcttccaga
     2341 ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac agcaatgagg
     2401 acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg atgattttga
     2461 tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa aactatgtca
     2521 agggggactt gaaagcagct tctcgtctcg ttcaggacag tctcacccta ctctttatgt
     2581 tcttgctacc agcaacggtt ggagtggtta tggtaggaga gcctctttat acggtcttct
     2641 atggtaagcc agatagtttg gctatgagct tatttgtctt tgcagttttg cagtctatta
     2701 ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc aaccgcaagg
     2761 ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta cctaccatcg
     2821 ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc attcctaacg
     2881 tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg attttgaagc
     2941 gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga accatccagt
     3001 ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt tatgtagctc
     3061 ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc tatttgttag
     3121 ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag ctttcgtaag
     3181 gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact tgaaacttat
     3241 tcctt
//
LOCUS       DAVMEF010000163         3210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVMEF010000163 DAVMEF010000000
VERSION     DAVMEF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.163"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..327
                     /locus_tag="VYQ30_001787"
     CDS             7..327
                     /locus_tag="VYQ30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966848.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(358..696)
                     /locus_tag="VYQ30_001788"
     CDS             complement(358..696)
                     /locus_tag="VYQ30_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966849.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(708..1427)
                     /gene="trmD"
                     /locus_tag="VYQ30_001789"
     CDS             complement(708..1427)
                     /gene="trmD"
                     /locus_tag="VYQ30_001789"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1966850.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(1417..1935)
                     /gene="rimM"
                     /locus_tag="VYQ30_001790"
     CDS             complement(1417..1935)
                     /gene="rimM"
                     /locus_tag="VYQ30_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1966851.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(1966..2754)
                     /locus_tag="VYQ30_001791"
     CDS             complement(1966..2754)
                     /locus_tag="VYQ30_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1966852.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
BASE COUNT          934 a          716 c          524 g         1036 t
ORIGIN      
        1 gccaatatga gacataaatt ccagcaagtt ctaaataaaa tacatgattt tttaaatgga
       61 tatgaccaac ctgaccagac tgaaaccaac tcccttacag ccactattga agaggctatc
      121 cagaaacaaa ccgctgttca ccttatctta tctgagacaa gctttacagg tgacatcatc
      181 aaatatgatc agcaaggcca gcaaattatc gtgaaaaatt tttccaaaaa tgtgagccgg
      241 attatccgta taagcgatat tcaacgcctg cgatttgtcc cctcaactgt ccaaacagcc
      301 caaaaaaata gatttaagaa agagtgagat gtagttgctt catcccactc ttttttctta
      361 gcgaatttgt tcaaaatgta aatgaactgc gatatgatct ccataaccac ttctttccaa
      421 gtcacgttgt aaacgatagg aaatgtagtg ttctgcaatg gtaatgtaac ctgcgcccaa
      481 taaacgatgt tcaaccatag attgaatcat actgatagtc gcacgttcca ccttggcttc
      541 ttgtaaatcc aaaactacct tcttagtgac ttgagcaaga ttttgacgca aatcatctgt
      601 caaaacataa acagtttggg ctgccttcaa gatggcttgg taaatcttat ctggattaaa
      661 ttcagcaatt tcgccattac gtttgattac ttgcataggt ttctccttta ttctttgttt
      721 tctttgattt ctgccagcat tttttcttct tctactgtca gttgataatg ttcaagtaaa
      781 tccggtctgc gctcgtaggt tttctttaaa ctctcgtaca atcgccactg acgaatcttt
      841 tcatggtggc cactcatcaa tacatctggc acgaccatgc ctcgataatc ataaggacgt
      901 gtgtactgag gatattctaa aagacctgaa gaaaaactat catcttggtg gctagactcc
      961 ttgccaatca cttctggaat caggcgaact gtagcatcaa tcatggtcat agctgccaat
     1021 tctccaccag tgaggacata gtcgcctagg gaaatctcat ctgttaccaa ggtcttaatg
     1081 cgctcatcat aaccctcata gtgcccacag ataaagatta gctcttcctc ttgagccaaa
     1141 tcttcagcat aagcctgatc aaactgcttt ccagcaggat cgaggagaat aacgcgcgga
     1201 tttttctttt caatagcatc aaaggaatcg aaaataggtt gtactctgag caacatgccc
     1261 tgaccgcctc cgtagggctc atcatctaca tgacgggcct tttcagcatt ttctcgaaaa
     1321 ttatgatact ggatatccaa gagccctttt tctcgagcct ttccaacgat tgagtgctcc
     1381 agtggagaaa acatctctgg aaagagggtt aaaatatcaa tcttcatcgt ctaacccttc
     1441 taagatttcc acatcgaccc gtttatttgg aatatcaaca ttgagaacca ctggtgggat
     1501 ataaggtaaa agcaaatcac gtttgccttt tcgtttgacc acccagacat cattagcacc
     1561 tggttgcagg atttccttga tggttccaac caagctatca ccctcataga cttccaaacc
     1621 gataatctcg tgatagtaaa attcaccatc gtctaggtca ttcaaatctt cctcagcgac
     1681 cttgagactg tatcccttgt acttttcgat agtattgata tggtacatat ctttgaattt
     1741 aataatgtca aagttcttct gtttacggtg gctagcgatg gtcactgttt ggacaaactg
     1801 atctttttca tcaaacaaag ccagctcagc tcctttttta aaccgttctt ctgcaaaatc
     1861 cgtcacagac aagactcgca tctcaccctg taatccctgc gtattaacga ttttcccaac
     1921 attaaagtag ttcatcttgt ctcctgtaat ctcctttttt ccatcttatt ctaacaattc
     1981 tcgaataata gccgcaattt tttccgattc tgaccattgt aaataatggt gattccctcc
     2041 taaaatgagt ttagtattgg aagtccaata ttctgattct ctgtactctt tttctctata
     2101 aggctgacaa aaaacaaata caggaatatg agcttctata gatacatcct caaaatcttc
     2161 ctcagtaatc tctccagata tctgaaattc tggatcttga ttttccaact ctaagccttt
     2221 ttcttgcatt aattcccaga tttttttatt cgtttcagga ctaaatgttg cttgagttaa
     2281 gttcttaaaa taaagttcag gaccacactc gtcaatcagc ctcatctgct cttccatttc
     2341 tggataagga ttttctgaaa aatcagcaaa catgactttt ttagttgtcg gttcaattgc
     2401 tactaaagtc tgacgcttaa ttggtttctc gagtaatttg caagctaaaa ttccactcca
     2461 actatgtgca caaagtatat attcagaaat tcctaattct tcaagtactt cataaaccgc
     2521 atctgcaaga ttatctagat tttttccagc ttggtcatga atcggactcc tacctgtgtt
     2581 cggaaaatca attgtcaaat aaccaattgt aggaggaggt ttttcaagta taagtgaaaa
     2641 attttcataa cttggtagca aacctgctcc gtttagacaa actagcactt tcttttgctt
     2701 ttgataagta acagagaggc taccaatttc tgtagatact tcaaacctct tcataaagaa
     2761 atccactgat tctatataat gaattattaa aaatccttat cctttatttt atcacgttcc
     2821 aaggattttc tcaagttgga ggaaggggac aatatctcta ctttcccttc aataatcctt
     2881 ccaaattatg tttatgttgg taattaacgg ctgcggtttt gtctttctca aagacagtct
     2941 tggtaaggtc aatatgatta atagctacga ttgcgacggt gtagtaaatg atatcagcca
     3001 gttctctggc aagttcctcg ttcgaatcct atcccttctt ttcgaccaga gcgcctattc
     3061 aaaacctcgg ctacttctcc gacttcctcc actaacttca taaaaagacc ttcatcagtc
     3121 cgagactgct gttaatgttc gattaagtag tcttggaatt gcctaaacgt tcaatctttt
     3181 atagtatatt gaaactagaa tagtacacct
//
LOCUS       DAVMEF010000164         3186 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVMEF010000164 DAVMEF010000000
VERSION     DAVMEF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.164"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..743
                     /locus_tag="VYQ30_001792"
     CDS             <1..743
                     /locus_tag="VYQ30_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY-like element IS1202 family transposase"
                     /protein_id="HEV1966853.1"
                     /translation="LANHGIPLQMKTDKRTVFTYQASNSKKMEDDTYTQFGYACHQLG
                     ILLETTSIPQAKGRVERLNQTLQSRLPIELERNKIHTLEEANTFLLSYIQTFNEQFGN
                     KTKLSVFEEAPNPSERNLILARLAERVVDSGHHIRFQNRCYIPTEQGKEVYFIRKTKA
                     LVLKAFDGDIYLNIADKIYHTKELLDHELYSKNFEQEPEQKKERRKYIPPQTHPWKLT
                     SFKQYLHKNKKDYEEFTSEEIHSPQLQV"
     gene            complement(1003..1755)
                     /locus_tag="VYQ30_001793"
     CDS             complement(1003..1755)
                     /locus_tag="VYQ30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1966854.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1752..3077)
                     /locus_tag="VYQ30_001794"
     CDS             complement(1752..3077)
                     /locus_tag="VYQ30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1966855.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(3098..>3186)
                     /locus_tag="VYQ30_001795"
     CDS             complement(3098..>3186)
                     /locus_tag="VYQ30_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1966856.1"
                     /translation="KEKDLQNIKGGEMKISRIILDFLFLRKK"
BASE COUNT         1083 a          626 c          470 g         1007 t
ORIGIN      
        1 tcttagccaa tcacggcatt ccccttcaaa tgaaaactga taagagaacg gtctttacct
       61 atcaagcatc caactctaag aaaatggagg acgacaccta tacacaattt ggatacgcct
      121 gtcaccaact agggattctc cttgagacca cctctatccc tcaagctaaa gggagggtcg
      181 aaagactcaa tcagacacta caatcacgcc tgcctatcga actggaacga aacaagattc
      241 atacattgga agaagccaat actttccttc tttcctacat ccaaaccttt aatgaacagt
      301 ttggaaataa gacaaaactc tctgtttttg aggaggctcc taacccctct gaacggaatc
      361 tcattctagc tagactggcg gagagagtcg tcgatagtgg acaccatatc cgattccaaa
      421 accgttgcta tattcctacc gaacaaggaa aagaagtcta tttcatcaga aagacgaaag
      481 cattagttct aaaagcattt gatggtgaca tctacctcaa tatcgctgac aaaatctatc
      541 acacaaagga gctactagat catgaactct attcgaaaaa ctttgaacaa gaaccagaac
      601 aaaaaaaaga aagacgcaag tatatccctc cacaaaccca tccgtggaaa ctcacatctt
      661 tcaaacaata ccttcataaa aacaaaaagg attatgaaga gtttactagt gaggagattc
      721 attctcctca actacaagta taatctattt ttcgctaaat gggaagcttt tccttaaagg
      781 aagtcactct atcccttaca tttaggagag ggaaagtgag gagtgtaaga caagtatttc
      841 atttgataag aggcttggag ctgttaggct ggcgagccaa ggctaagact agccttggtt
      901 tagctaacaa aacagcttca agggttcctc gtgtcaaatg aaatcaaaat aattgccctc
      961 aaactctcca ttttttgggg acataatcac tttcgattga catcactttt gagatttttt
     1021 ctctaaaata tcttttaatt ttctaatttt taatcttgaa ataggacaac gatggtcttc
     1081 atagaaaaca atttctaagt tttttcgatc aatttctctg atattgccta tatttaccaa
     1141 aaatgacttg tgaggagaat aaaatcgctg agcatgtttg tccttttcct gaatatctgt
     1201 catggtacca taaaactctt ttgcaaaatt cttaccaata atgcgcaatt tatgagagac
     1261 tcctgtcgtt tcaatataca aaatatcatg gtaaggaatt tttaaatcat ttcccttgta
     1321 attgtagtcg aaataatcta caacatcttc attttcaagt aacatactct tcgtgtagaa
     1381 gatattttgc tcaattcgct tcttaaacat ctcatcgttg atatccttat caacaaaatc
     1441 tagggctgat atctggtatt tataggtcag agtcgcaaac tctgatcgac tagtgataaa
     1501 gacaataata gcgtaaggat tgtaatgacg aatgagctga gccacttcaa atcccttttt
     1561 ctcaattcca tgaatatcga tatctaggaa ataaagctga tttacttcat cattttcaat
     1621 gtattcttca aattcacgga cttttcccgt tgtcttgtat gatattggaa tattcgattc
     1681 tttcgaaatt tcatccaata ttctctctag tctcacttga tgttcaataa catcttctaa
     1741 aattaaaact ttcattcaaa ttccctctta aatctaatga tttgtctaaa tgtactgcct
     1801 tccatctctg tttctaaaat aatattgttg tacttatcta gtagttcttt cacattattt
     1861 aatccgactc cgcgatttct tcccttagtg gagaatccta aggcaaatag atctcctgaa
     1921 ggagtcatcg tcattttaca tgaattctga atcaccataa ctgtttcagt ttccatctta
     1981 ataactgcta cttccatctg ctttttatag ctatcagccg atccttcgac agcattattc
     2041 aataaaacgc tcatgatacg aaccaaatcc aatagttcaa ttggaagctt ggtaatcgta
     2101 tcttttactt ccagtgtaaa ctctacacca ttatttcgag catagacaat tgactgagca
     2161 accaaacttc gtaaagctga gtcttctatg ttgttcaaat caaagtaagt gtacttatct
     2221 gaacgcaatt tatgatttgc tttgactaaa acttcattgt aaattctgtc aatttcctgt
     2281 aaattaccac tgtcaattgc catctgcatg ctgacaagca ttccagcata atcatgtcga
     2341 aaaccacgga tttcattata cagaccaaca atttcatctg tgtaattctg taaatgtttc
     2401 tgttcaaatt tcttctgctt caaagcaatc tctttctcca tttgaacttt atgagaattc
     2461 attgcaaaga aggtcaaaag gagagagata aagacaatag atgacaaaat acttccaaaa
     2521 ctattcaaat gtttaatcgt acttaccata tctgaaacga aagatacaat atgtagcaat
     2581 agtaaagcaa aaaatacttt tttcaagaaa ggataaaggt agtccttgtc aaaataggct
     2641 agttccaaat ggaaataata aatgattttt aatgtaacaa aataggttaa caccgtcaca
     2701 acgaaaaaga atgggaaatg atattgtaaa acaaaattat ctcctgttat agaggagaaa
     2761 gttacggaca gaaagttatg agtgctctca tataaaagag atagtagtaa acttaggaat
     2821 agtcctctat ccctctcata ctgtttcatc catcgaaaat aggaatataa gcccaaagga
     2881 aataaaaatc tttcaatccc tattttatct aaatatagaa gataaaagga aaattcaagt
     2941 actatttcag ttagtaatgt ataagcacca aaaatgtata attcttttct atttattcga
     3001 cctttacaaa ttaaacggta actgtgacta ataattaaaa aatgaacaat aactgtccca
     3061 aatccaagta aatccattac tctttctcct tatttcatta cttttttcgt aggaaaagaa
     3121 aatcaaggat gattcttgaa atcttcatct ccccaccttt aatattttgt aagtcttttt
     3181 ccttca
//
LOCUS       DAVMEF010000165         3106 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVMEF010000165 DAVMEF010000000
VERSION     DAVMEF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.165"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1028)
                     /locus_tag="VYQ30_001796"
     CDS             complement(<1..1028)
                     /locus_tag="VYQ30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1966857.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMALAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDP"
     gene            complement(1041..2879)
                     /locus_tag="VYQ30_001797"
     CDS             complement(1041..2879)
                     /locus_tag="VYQ30_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1966858.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(2897..>3106)
                     /locus_tag="VYQ30_001798"
     CDS             complement(2897..>3106)
                     /locus_tag="VYQ30_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1966859.1"
                     /translation="FAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTE
                     YNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1039 a          634 c          422 g         1011 t
ORIGIN      
        1 attggatcaa tttgccatcc ccactctgaa gactctaaat aaggatttac taaaccacca
       61 ataatattcc cttctcctga attatactgt gttggaatag cagattgagt cacactcatg
      121 taatagctaa aggataaaaa atctacagta taatttttta ataactctgc atcttcagct
      181 gcaaactcta tgttaatgtc atgttcctta aaatatcttt ttgcataatt cggataataa
      241 cctctaacat gcacatctga aaatagataa tttagattct catactcatg agtcgcccat
      301 acatcttttg gatttggagt cattggataa gctggcatag ctaatgccat acatcccacc
      361 ttaaactctg aattaatctc acgagcaatt tttgtaacca aacttgaggc gactaattca
      421 tgatgtatag cttgatataa ttcttgtttc gaaagattct ccttaggtat atctattcct
      481 ccactagtaa atggtaattc caaaacagag tttacttcgt taaatgtaag ccaatattta
      541 actttatctt tatacctttc taaaactgtt cgagcaaatt tttcataaaa atgaatcatt
      601 ctcctatcaa cccatccatg atattttctt gctaaatata atggagtctc atagtgtgaa
      661 agagttacaa gtggctctat cccgtgagca tgtagttcat caaacaattc atcataatat
      721 ttcaaaccag cttcgttagg ttcttcctca tctccttttg gaaaaattct actccatgca
      781 atagaagtac gaaaaacatt aaagcccatt tcagaaaaca aggatatatc ttccttatat
      841 ttatgataaa aatcaatacc taacaatttt aagttatctt ctgtaggatt ttctgttgct
      901 tctcctaatc cacctttggg taacacatcc tgaactgata agcccttacc atcttcatta
      961 tatgctccct ctacttgatt agctgcaaca gctccacccc aaagaaaatc atctggaaaa
     1021 atggtcataa ctttcctcca ttataatatt accagtaatt ccttagaatg ctcgattgtc
     1081 tgattattag gtaatactaa tacatctaga aaatcattgg tattcgttac aattactggt
     1141 gtaactgttt cgtagccttt agtcttgatt aaattcaagt ccatttcaaa aatcaactga
     1201 tttttgaaaa ctctgtctcc ttcttctaca tgactaataa aaccttgacc ttttagctca
     1261 acagtatcta atccaatatg aattagtaac tcaacaccct catcactctt caatccaatt
     1321 gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa atggtgcata aaccttacct
     1381 tcacttggga taatcgctac cccgtctcca attagtttat ctgaaaatgt tttatcctgg
     1441 acatcgctta acggaatgat ttctcctgat ataggagaaa atatcatttt tttatttgaa
     1501 actccagctt taacttctaa attgctagaa ctctcttctt catcgattcc aaatatataa
     1561 gctaatacaa aggtaataac aaccgaaatg accgccacaa ttaaagcatt tacaatattt
     1621 gatggcacat cagaataaat aaattgaggc aacgctatca aagatgggac agcaaataga
     1681 tatgctttaa cactagtaag acctgcaaat aatcccgcta atccaccacc aatcatagct
     1741 gcataaagcg gttttttata ttttaaagtc acaccatata atgcaggttc ggtaatccct
     1801 gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt atttttactc
     1861 tttaatgcaa cagccatcga agcagcccct tgagctaagt ttgaccctaa cattgctgga
     1921 agaattaata cgtctggagt agcaatagat gccgccaaaa aaataggtgc aaaagcccaa
     1981 tgcattccag tcataacaat aaatggcata atagcaccaa gaatagctaa tgtaagccat
     2041 ccagctacac catacatttg cccaactaga tttgataatc cttcaccaac aattactcca
     2101 ataggtccga ctacaactaa ggcaatacag cttgatacta ataatactag cgtaggttgc
     2161 aaaaaactct tagtaatagc tggtgttaat ttagcaatta ttttttcaat atatttcatc
     2221 aaccaaacca taataagaat tggaacgact gatgaaccat aactagctgg tgtcacaggt
     2281 gcaccaaata aactaagagg attccctgat tgcaccattt gaacaaaatt tggatggaga
     2341 agtacacctg ctacagacat agctaatgta gatgttactt ttaatttttg tgatgcagaa
     2401 taagctaata acagcggtaa gaaataatat ggagcatccc caaaaaatgt caaaaaagca
     2461 atagtctgag aatctgattg caatatacca agcattggta aaatgattac caagactttc
     2521 aacatacctc cccctaacat tgctggaatg attggagtca tggaaccagc gatatactca
     2581 atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa ctgaatcgga ttcaaaattg
     2641 ccaagtttaa cgaattcttt ataataatta gctacatcat taccaagtat aatttgatat
     2701 tgtccattct ttttcataat acctattaca cctggtatct tcttcacatc atcatcattg
     2761 actaaatttt catcttttaa ttctaatctt aaacgtgtta cacaatgggt aactctattg
     2821 acattttttt cacctccaat tacatcgagg attttttgta ccgtatcttt ataactcatg
     2881 gtattctcct attctattaa tctaaatttt ttgttaagcg acgaatatga gccatcaaat
     2941 aaactaattc actagaagtc agcaaataat tgtactctgt ttgtataaac attgccacct
     3001 gttcaccaca ttcatattct ctaggatatt tatttttcat taatgctaac aagtcttcat
     3061 catcatcgtc acaataagtt gtattttcaa ccaagcgatg ggcaaa
//
LOCUS       DAVMEF010000166         3095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVMEF010000166 DAVMEF010000000
VERSION     DAVMEF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.166"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..1243
                     /locus_tag="VYQ30_001799"
     CDS             17..1243
                     /locus_tag="VYQ30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1966860.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            1442..3016
                     /locus_tag="VYQ30_001800"
     CDS             1442..3016
                     /locus_tag="VYQ30_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1966861.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT          871 a          580 c          699 g          945 t
ORIGIN      
        1 gaaaatagga ggttttatga aatcgaatcg ttatattatt gcctttgctg gggtcatttt
       61 acacttaatg ctgggttcga cttatgcttg gagtgtttat cgtaacccta ttattgaaaa
      121 aacgggatgg gatcaggctt ctgttgcctt tgcctttagt ctagcaatct tttgtttggg
      181 cttatcggct gcatttatgg ggcgtttggt agaaaaattt ggtccgagag tcatggggag
      241 tctatctgct tttctatacg caggtggaaa tatcttaaca ggatttgcaa tagaccgtca
      301 ggagctgtgg ttgttgtatc tcgcttatgg cattttaggt gggcttggtt tgggagcagg
      361 ctatattacc cctgtgtcga cgattataaa atggtttcct gataaacgtg gtctcgcaac
      421 aggtttagcg attatggggt ttggttttgc ttctttattg actagtccca tagcgcaaca
      481 cctcatcgca ggggtagggc ttgtagaaac tttttatatt ttaggtgcaa gttactttat
      541 tatcatgctc ctagcttcac aattcattaa gcgtccaaat gagcaagagc ttgcaatttt
      601 atcttcttca gggaaagaaa aaacagcctc tttgacgcaa ggaatggctg caaatcaggc
      661 tctaaaaagc aatcggtttt atatgctttg gattattttc tttatcaaca tagcttgtgg
      721 tttaggctta atttcagcgg catcgccaat ggcacaggag atggctggct tgtctacaag
      781 tcatgcagca gtaatggtgg gtgttttggg gattttcaat ggatttggtc gcttgctctg
      841 ggcgagtttg tctgactata tcggtcgccc tctaaccttt agtatattac tgcttgtcaa
      901 tcttttcttt tctctctcac tttggctctt tacagattcc gttttatttg tagttgctat
      961 gtctattttg atgacttgct atggagctgg tttttctttg attccagctt atctcagtga
     1021 tatttttgga accaaggaat tggccgctct gcatggttat attttaacag cttgggcaat
     1081 ggctggttta gcgggaccta ttttattagc agagacttat aaaatggctc attcgtacac
     1141 acaaaccttg ttcgtttttc tcattttata cagtatcgcc ttggctttgt cttattatct
     1201 aggtcgttca atcaaaaaag aaagtcaaaa agcgcttaca tgattttctg tagaaatggg
     1261 gctatctttt gctctggtct tgtgttatac tagataggtt gcaaagaaaa cagtactttt
     1321 cttttgtgga aaagaagcaa cacgatttta tatctagtta tatgaaaata cgtcataaaa
     1381 agaaaagtat aaactaagcc ctatagggtg gcttcgcacc acctttagaa agaagaataa
     1441 cgtgaaattt aatgaattaa acttgtctgc tgatttgcta gcagaaattg aaaaagctgg
     1501 ttttgtagaa gctagtccta tccaagaaca aactattcct ttggcccttg aaggcaagga
     1561 tgttatcggt caagctcaga ctggtacagg aaaaactgca gcctttggct tgcctaccct
     1621 tgaaaaaatc cgtacagaag aagcgactat ccaagccttg gtcatcgctc caactcgtga
     1681 actagctgtc caaagtcaag aagaactctt ccgctttggt cgtagtaagg gagtcaaagt
     1741 ccgttcagta tatggcggat caagcattga aaaacaaatt aaggctctta aatctggtgc
     1801 ccatattgtg gtgggaactc caggtcgcct cttggacttg attaaacgca aggccttgaa
     1861 attacaagac attgaaaccc ttatccttga cgaagcggat gaaatgctta acatgggctt
     1921 ccttgaagac atcgaagcca ttatttcccg tgtacctgag aaccgtcaaa ctttgctttt
     1981 ctcagcaact atgccagatg ccatcaaacg tatcggtgtt cagtttatga aagcccctga
     2041 acatgtcaag attgcggcta aggaattgac aacagaattg gttgaccagt actatatccg
     2101 tgttaaggaa caagaaaaat ttgacaccat gactcgtctc atggatgtgg cacaaccaga
     2161 actcgctatt gtatttggtc gtaccaaacg ccgtgtggat gaattgactc gtggtttgaa
     2221 aattcgtggc ttccgtgcag aaggaattca tggcgaccta gaccaaaaca aacgtcttcg
     2281 tgtccttcgt gactttaaaa atggcaatct tgatgttttg gttgcgacag acgttgcagc
     2341 gcgtggtttg gatatttcag gtgtgaccca tgtctacaac tacgatattc cacaagatcc
     2401 tgagagttat gttcaccgta tcggtcgtac aggtcgtgct ggtaagtcag gtcaatctat
     2461 tacttttgtt gctccaaacg aaatgggtta ccttcaaatc attgaaaact tgactaagaa
     2521 acgcatgaaa ggtctcaaac ctgcaagtgt agaagaatcc ttccaatcaa aaaaacaggt
     2581 agctctcaag aaaatcgaac gtgattttgc agatgaaacc attcgtgcca actttgagaa
     2641 atttggtaag gatgctcgca aattggctgc tgagtttact ccagaagaat tggcaatgta
     2701 tatcttgagt ctgacagtcc aagacccaga tagccttcca gaagtggaga ttgcacgtga
     2761 aaaaccacta ccgtttaaac catcaggtaa tggtttcggt ggtaaagcta agggaggtcg
     2821 tggaggccgt cgtggggacg accgtcgaga gcgtgatcgc cgtggcaatg gtcgccgtga
     2881 tgagttcaaa aaaggaagtc gtggcaacga tcgttttgat aaggaaaaac gttaccgtaa
     2941 ggataataaa aaaccacgca atactttaag cgaaaagcaa acaggctttg ttattcgtaa
     3001 caaaggcgat aaataagaaa aagcggttca aaatgaatcg cttttttata taaggagacc
     3061 gaatgtgaaa agacaaataa aattattata ttatc
//
LOCUS       DAVMEF010000167         3085 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVMEF010000167 DAVMEF010000000
VERSION     DAVMEF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.167"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..174
                     /locus_tag="VYQ30_001801"
     CDS             <1..174
                     /locus_tag="VYQ30_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1966862.1"
                     /translation="NRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTL
                     ITGKNGTGKSTLL"
     gene            complement(540..1850)
                     /locus_tag="VYQ30_001802"
     CDS             complement(540..1850)
                     /locus_tag="VYQ30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1966863.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEDQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2086..2958
                     /locus_tag="VYQ30_001803"
     CDS             2086..2958
                     /locus_tag="VYQ30_001803"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1966864.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          949 a          518 c          704 g          914 t
ORIGIN      
        1 aacagaactg tttcttttgc tattagttca aaaggagaaa aaatgaaagt agaaaatatt
       61 tcgtataggg tggatcatcg tatattgttt gataatattt cttttgatac ttcgagttca
      121 ggcgtgacat taattactgg taaaaatggt acaggaaagt caactttact atagtagatt
      181 gaaactagaa tagtacacat ctgcttctaa aatattgtta gaaatcgatt tgactatcct
      241 gatctatttc tcctgttctt atttcatttc actacatctc aaattgagta tgacgaagtg
      301 cgctcccatg tcctgggaac gcactttctt catatttttc atattcttga atccatcgat
      361 aaagactatt gggatgagtt tttaaagttg aactaatcat ttttacagga tgaggtttac
      421 agcagagagt ttgaaggctt tatcaaaggt ttttcttggc ataatgactt ttcctcgttt
      481 ccgcttaatt ttgtgtctac tttatgatac caagtccacc cttaagttag ataataaatc
      541 taacttaagg aagctagaag gatgagaatc caggtggtca agagtcccaa acttaagctg
      601 atggggacac ccagaataat ttgctttttg aaggcaaggc cacgttcctc tatattggga
      661 agtgagagtt gaatgagaga accagctgat gaaaagggtg agatattagt agatagagcg
      721 ccaataacgg tggctgttgt gagtaagtga atatcaatct gaggattttg agcactgatg
      781 atagcaatga tgggaaagag ggctggagct acaacggata gggtggaact aaagagtgac
      841 atcactccgg ctatcacaca aaagaacaga ggtaaccaga aatgaggaat ggatgttgtc
      901 atgaggtgcc ctatcagtgt gactaaacct gacttgaccg ctagagacat tagtaagctc
      961 atgccgcaga gcatgataat tgtagcccag ggaactttag ccaaaatggc ttcttgcttc
     1021 cctaatttga gccttaaggc gaggcagacc ataagtattg agacaaagcc aatatcaaat
     1081 gttttttgat aagtagctat ccaggcgatg tttgggaaaa tgagatgcaa caagggaaaa
     1141 agccaaacca aaaccatgct gctgatcatg agcaaggtgg tttgtctttg aaccttgctg
     1201 aggagtggtg gttggtcaat agtcaaggat gagtttgttc ttcccttact atagtgactg
     1261 taacaggata ataaaagcaa gacgatgagt gggtagataa tgctgacgat aaagatatga
     1321 ttgccaagtg aaaaagcttg atcttcccat cccatttgct taaacaggcc ttgaaagaca
     1381 atgcctgagc tactggttat caaattagcc cctcctgaag ctccccaatt gacggcttga
     1441 gctccaatca aagggtgttt gtccgctttt tgacagaggg taatcgctag aggacagcaa
     1501 acggccatag tagtgaaaaa tccagcacct aaagcagaca aaagggttgc catcaggtat
     1561 aaaatcatgt agagggcgtt agggtaggtg cgtgtgcggt agagaatgtg ttgagccaaa
     1621 acatcaagag taccgttagt tattgcaaag ttataaaaga gagagacgct aaaaatggta
     1681 aaaaagagtg aggttggcca aaaatgaaga agttctttgg ggcttaatcc catgagagtg
     1741 gttgcgatga ggtaagaaaa agcaatagcc agcaggccaa tattgatttt ggtgcggtaa
     1801 ccaattccaa tggctagagc aatggcgcta atcattatta aatgaatcat tgaattgtcc
     1861 tttcagttag aatatggaaa taggttggca attttggcgc tcaaaagacc acctgtttcc
     1921 tattggtact tgactattat acacaaaaaa atacaattgt cttgacaatt gtattgacac
     1981 ttgttagaat taaaaatgag gtattcaggc ttgtgatttc ctatggaagt taatagtgat
     2041 tgcctctaat gcttacaagt gatattaaaa atagaggacc tagtgatgtc aatcatttca
     2101 actgatttaa ccccttttca aatagatgat acattgaaag cagccttgcg agaagatgtt
     2161 cattccgaag attacagtac caatgccatt tttgatcatc atggccaagc caaggtgtcg
     2221 ctttttgcca aggaagctgg tgttttagcg gggctaaccg tttttcaaag ggtttttacc
     2281 ctatttgatg ccgaggtgac cttccagaat cctcatcaat ttaaggatgg ggatcgtttg
     2341 actagtggcg atttggtttt agaaatcata ggctcggtga gaagtctctt aacatgtgaa
     2401 cgcgttgcct tgaatttttt acaacattta tcagggattg cttcgatgac agctgcttat
     2461 gtagaagcct taggcgatga ttgcattaag gtatttgata ctcgaaaaac tactcctaat
     2521 ttacgtcttt ttgagaaata tgcagtgaga gttggcggtg gctataatca tcgctttaat
     2581 ttatcagatg ctatcatgct aaaagacaat cacattgcgg cagtaggtag tgttcaaaag
     2641 gcaattgctc aagcgcgtgc ctatgcccct tttgtgaaaa tggtcgaggt ggaagtggaa
     2701 agccttgctg ctgccgaaga agctgcggcg gcgggtgctg atattatcat gttggataat
     2761 atgtcattgg aacagattga acaggccatt accctaattg caggacgttc tcggattgaa
     2821 tgttctggaa atattgatat gaccactatt agccgttttc gtggtttagc gattgattac
     2881 gtctccagtg gtagtttaac ccatagtgct aagagtcttg atttttccat gaaggggtta
     2941 acctaccttg atgtctaagt tgtaaaataa actaactttt taaaggatgt ctttcctcta
     3001 gaacgagttt tatgtcagat agtttaaacg cctcttcaaa tatagtaaaa tgaaccaaaa
     3061 atagtacaca atgtggtata atctt
//
LOCUS       DAVMEF010000168         3028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVMEF010000168 DAVMEF010000000
VERSION     DAVMEF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.168"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            382..1577
                     /locus_tag="VYQ30_001804"
                     /pseudo
     CDS             382..1577
                     /locus_tag="VYQ30_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1956..2741)
                     /locus_tag="VYQ30_001805"
     CDS             complement(1956..2741)
                     /locus_tag="VYQ30_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966865.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(2743..>3028)
                     /locus_tag="VYQ30_001806"
     CDS             complement(2743..>3028)
                     /locus_tag="VYQ30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1966866.1"
                     /translation="KNLWGSNLLKNSLVAFLSGSLVPLTFFPKIIAELLSFLPFSSLI
                     YTPVMVIIEKYSMSQMIQALSLQLFWLFIMIALSQLIWKCVQNYITIQGG"
BASE COUNT         1081 a          588 c          516 g          843 t
ORIGIN      
        1 aagttactaa aaaaagattt tggaataaac tttttcatag cgataaaagc accgtacaag
       61 ctaggaaaat aataagtaat cgcccaatct gaccttctcc agaacttgat tattcgtcta
      121 tcactacagt tgacaaacat actaagatta gtagtgagga aatctccaac gggagagagt
      181 actcattact ttttctttgt gttaaaatag tggtgtcttg ttaagtcata gagctctcta
      241 tggtgctaga cgtcgcttga caaactggca agaaacctgt tttaggaaga attttatcaa
      301 agtatgtggg atactagacg tcgagtattg gaaataaaat cactaaaaca aggagtcatt
      361 caagacaaag gggtaaacat gatgcggaca gttttcagaa tggatgtgag taaggcaagc
      421 tcagaagtcg ctattcttgt gaatggtgaa aaggttcatg gctataccat gcccaatgat
      481 gccatcggat ttagtcgtct tctagaggat ttgaaatagg taaatacacc tgagattatc
      541 ttcgaggcac aggggtctat tctcgtcttc aggcctttct ggaggacaat agctatgctt
      601 atatacggct aaatccttta aaactaagaa gcaactagac agtttgcgag tccgtaaaac
      661 caatcagatt gacgctgaaa agctagcttc gtcacaattc atccttaacc gcaaaccaac
      721 ttatgttcaa gaagaagtct atcaaaattt gcgtgacctc agtcgattct atcagaatct
      781 gacagaagac atcgttagga ctaagaatag cctgcacaag gtgcttcaaa ttgcctttcc
      841 agaaataaag aaccttctct ctactcctac aggggaacaa tattggaacc tcgtgaccat
      901 ctttccaacc aagcattgga ggctgaaatt atcagtagtt gagctaaaag aagccatcgt
      961 aattcaactt ctaaacggat ttctgagaac agagttggac gattggctga aaagttgagc
     1021 gagctggccg aacagtccta tcttgccatt tcaaaaacct ctcccatgac agaagaggtt
     1081 aagtatcatg cacaggaact gttgagactg acaaaccgca aggatcagga cttagcacaa
     1141 atgattgaat tggctgaacc attacctgaa tacgagattc tgctctctat tcctggtatc
     1201 gcagaaacaa cagctacaag cattattggc gaattggaga cattcgtcgc tttcagtctg
     1261 ccaaccaaat caatgccttt atcagtattg acctcagaca ctatgaatct ggcaatttcc
     1321 tagctcaaga acatatcacc aagcgtggaa atccttacgc gagaaaaatt ctctataagt
     1381 gcgtccacaa tattgcttca gctagtcata ccaatccttg tcatatcgcg gacttttatg
     1441 agaaacgaaa aagacaatcg cagacgactt caaccaagcc atatacgatt gtctctatac
     1501 atcgcctcat ccggacaatg tattacctca taacgcataa caaactttac gactacactt
     1561 taacccaaag tcattaaagt catctatgtc ctatcattgt aacaccttat tttttaatac
     1621 cagataagtg tcttttaagt gtgcccaaat ttcaaaaaac agctcgcaat tatcttctaa
     1681 acattccttt gatactcatt cagaaaacct tttgagctag aatatcgtat gtcccttaac
     1741 aaatggtatg tataaaagag ctaaaaaacc actcatgata tgaacgagtg gttatgactg
     1801 aaatattaca tacctttgta tcatttttgc acaatggtca aatttcttgt ttgaaaaact
     1861 acagtaagcc taatcaaagc tctcaatgta tcattaaaac ttccttccaa agaataggaa
     1921 gttctatcaa aattcttaat tagattttac ttcttctaag accctgcgct ctcatatgca
     1981 tccaaacctt tgttccataa ttttaatgat aaggtaaaaa atattaatga gataaggatc
     2041 aatccaccaa tattgaacaa tccatccttg tctttcaaga aatagctagc tgggtaatag
     2101 gctgtaaaag caaaaggaat gataaaacta atcaaccaac gtaaaaacga atggtaaatc
     2161 gcaatcgggt attttgcaaa atcattaaac atgtaaaaaa tataaattat tgccccagat
     2221 tgtttagtcc aaaaagcaat actagctgtt acaattttta aagaggtata aattaaggtt
     2281 gcaaagggaa tactgattaa aaagagaaaa acctttgccc aagtccaagt aagactagtt
     2341 attgtcatta acagcaataa gacgcctact aacaattctc caagagcatc aatttgaaac
     2401 gtttctacta aaatatgaaa caaagggcta atgggtctag tcaaatactt atcaaattct
     2461 cctttacgaa ttaaatgttg tcctaaagcc cacagattat caaaaaagag atggtcaatt
     2521 cccttaggaa tgagagaaaa accataaata aaagcaacct gatgaaaact ccagccatca
     2581 agtaagggaa tatgttgaaa caaaatattt agaaacagca tgtttaaccc ttgtgtcaaa
     2641 aagacaccca caacaccaac taaaaaatca gccttatact ccataatctg tttaagatat
     2701 tgtcggataa aaatcaggtg catgcgttga tatttcttca ttctaacctc cttgaatagt
     2761 tatataattc tgtacgcact tccaaatcag ttgggataag gcaatcataa taaacagcca
     2821 gaataattgt agcgagagcg cctgaatcat ttgactcatg ctatactttt ctataataac
     2881 catcaccggg gtatagatta aagaagaaaa tggcaaaaaa gataatagtt cggcaataat
     2941 ttttggaaaa aatgtcagag gaaccaaact tccagataaa aaggcaacta aagaattttt
     3001 caaaagattt gatccccata agttttta
//
LOCUS       DAVMEF010000169         2982 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVMEF010000169 DAVMEF010000000
VERSION     DAVMEF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.169"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(25..657)
                     /locus_tag="VYQ30_001807"
     CDS             complement(25..657)
                     /locus_tag="VYQ30_001807"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1966867.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(691..1392)
                     /gene="pyrF"
                     /locus_tag="VYQ30_001808"
     CDS             complement(691..1392)
                     /gene="pyrF"
                     /locus_tag="VYQ30_001808"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1966868.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPV
                     AAYHAIKDEWTQDWN"
     gene            1713..1805
                     /locus_tag="VYQ30_001809"
                     /pseudo
     CDS             1713..1805
                     /locus_tag="VYQ30_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2127..2840)
                     /locus_tag="VYQ30_001810"
     CDS             complement(2127..2840)
                     /locus_tag="VYQ30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1966869.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(2833..>2982)
                     /locus_tag="VYQ30_001811"
     CDS             complement(2833..>2982)
                     /locus_tag="VYQ30_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966870.1"
                     /translation="DYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTG
                     GKLNA"
BASE COUNT          916 a          621 c          536 g          909 t
ORIGIN      
        1 ctcgtctctc tttatcttac tgacctaacc ttcttgccaa ttttcttggt cttctttaaa
       61 gcgttttaga agatcaaggc cctctggcgt gatgtaacct tcttcttggg ctagatggat
      121 aagctcgcta tagtttgaaa gcgtcacaag tttaacacca gcatctgcaa agttcttatc
      181 tgcttttggc aattggtagc tgaaaatcgc tacaactcca agcacatctg ctccttctcg
      241 cttggctgct gctacagctt caagaactga accacccgtt gaaataaggt cttcaaccac
      301 taccattttt tgaccttgag ctacgcgacc ttcgatttga ttaccagctc cgtggtcttt
      361 tggtttgcta cggatgtagg caaaaggcaa gtccatctta tcagcaataa tggctccgtg
      421 tggaatccct gctgttgcag ttcctgcaat cacttctact tcaggaaagg cttctttgat
      481 agcttccaca aaaccatttt caattagggt acgagtttct ggataggcta gtgtcacacg
      541 attatcagtg taaatcggtg acttgatacc agatgcccaa gtgaagggtt cctctggttt
      601 gaggtaaacg gcttggattt tcaagaggtg gctagcgata tctttagcaa gtgtcatggt
      661 attctccttt tatttttcta atctagttct ttaattccag tcctgtgtcc attcatcctt
      721 gatggcatga taagctgcaa caggctcctc agcttgggta atgggacgtc ccactacgat
      781 atagtcactg ccgatttgat aggcatcagc aggtgtcatg actcgttttt gatctccaac
      841 tgcaacacca gctggacgaa tccctggtgt cagacagata aaatctggat tggtagcctg
      901 cttgatgact tgtacttcct gagccgagca aacaacacca tccaagccag cttcagctgt
      961 cttcttggca tagtgaatca cagactcttg cagactggtt tggatatttt gaaactcctg
     1021 catctgagct tctgacgttg atgtgagctg agttacagcg atcaatttag cttgacttcc
     1081 aagaccttca cgcgcagcct tcatcatctc tacaccacca gccgcatgaa cattggtcat
     1141 atccacacca agctgagata ggatcttcat ggctgacttg actgtattag gaatgtcatg
     1201 aagtttgaga tccaaaaaga cactatgacc caaacctttt aagtaggaca caatctcagg
     1261 ccccgctgcg taataaagct ccatccctac cttgagataa aggctttctt ctgctgggaa
     1321 aagagctaaa aattccttga ccgcctcaaa actaggaaaa tcaagagcaa tgattggacg
     1381 atgttctcgc ataggtttct ccttttacct ttactagtga gagagtctgg ccaacagaag
     1441 ccacgaaaaa aggaacctgc caacagcaag gttccacgaa aaatgggtag tctccaccct
     1501 ttcaccgtct aaccttagct gcctctctgg actgctttaa aggttttcta ttattctatt
     1561 atttctacta gcacttgtca agtaaaaaca tggtcacgta tgtgttgact gctattgaac
     1621 acctcacaag actctttcca aaagaatagt taaagacttt cacttaggac agagaaaaag
     1681 agtttgcttg ttacccttta attgaacaaa tgggaattct gttttatttt gcggatgcct
     1741 attcatcttg gcaaagggga agtaatgaga atgcgaatgg acttcttcgt gagtattttc
     1801 ttaaaccgat ttggcaacaa ttaccgaaaa aatccttaat ggtgctttgc tagcttatta
     1861 atcaccgtcc ccaaaaatac ttggattaca agacaggttt tgaagcactt atagaggaga
     1921 tttaaaaatg ttgcattttt tattgcaatt tatcagttat cttatcaccc aaaacttctg
     1981 caattagtat gagcactatt gttatttttc ttttttcgta cactcaaatc aaaatatagc
     2041 aataagacta ttctgtagaa ttgctgtcaa aaagatcagt tcatttttcc ttcccacttg
     2101 tgtttataat cattaaaata actcaattaa gaaattttct ttttcctaat agttatcgta
     2161 attaaagtta atagaagaac aatccaaata agtagtgtta gattaggaat tagagtttca
     2221 ttccatgaaa atccctccca ttttgtgaaa aacttagtaa acagtcccat aaaactataa
     2281 tctgttatct tcgcaactgt cttatccaaa ccagatagca tcggtaagaa agcaactaaa
     2341 atcatagcag gaagactgat aacctgagct actacttggc tcttcgcggt taaacctatc
     2401 aataaataga ataaaatgat ggataaagag gttagaagaa cgattgtaat ataattaaaa
     2461 gtatgtacaa ttgtaactcc taaaataaga ggagtagttc ccataatcac aaaagttagc
     2521 aaaaaaggaa gaaacatcgt tgataaaatg tattcggatc ctttaacacc actcaacaga
     2581 agagtttgta aattgtactt ttctttttct tcagacaaga taatagttat aggacttcca
     2641 acagccaaag ccaaagaaaa aggtaaacac atcatcaaaa gaactaatgc ctgttgatcg
     2701 ttgaccttcc cctgtgtttc cataagatat ttataaaaat atgtgaaagc aaaaggcact
     2761 aaaacttgca ataaaatact cttattggaa aggataattt gacaacgcaa ccataccaaa
     2821 gccagaaacc gtttaagcat ttagctttcc tcctgttaat gtgataaaaa tatcttctaa
     2881 agtaggctca catgaatgaa ttgaaaaaat attttccaga tcagcctgag atacctgttc
     2941 tagtgatgta aaatcaaaag ttatctgatt cccattataa tc
//
LOCUS       DAVMEF010000170         2978 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVMEF010000170 DAVMEF010000000
VERSION     DAVMEF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.170"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..737
                     /locus_tag="VYQ30_001812"
     CDS             <1..737
                     /locus_tag="VYQ30_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1966871.1"
                     /translation="INRPLNDSQSFKTKSNIAQIEAQALKHLDKPIIDLSGWQRPEEI
                     NYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKSHITELQKRNVPVAVYAYV
                     AGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMNEGVENFRAKLASLGAKNI
                     GIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKTDLDYDIHQYTSKGKIAGF
                     DHDLDINVISPLKNKEETFRKLFLKP"
     gene            832..1674
                     /locus_tag="VYQ30_001813"
     CDS             832..1674
                     /locus_tag="VYQ30_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1966872.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            1674..2156
                     /locus_tag="VYQ30_001814"
     CDS             1674..2156
                     /locus_tag="VYQ30_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1966873.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            2156..2776
                     /locus_tag="VYQ30_001815"
     CDS             2156..2776
                     /locus_tag="VYQ30_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1966874.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
BASE COUNT          902 a          617 c          562 g          897 t
ORIGIN      
        1 tcatcaatcg cccacttaat gacagccaat cgtttaaaac aaaatccaat atagcacaga
       61 ttgaagcaca ggctttgaaa cacttagaca aaccgattat tgacctctct ggctggcagc
      121 gtcctgagga aatcaactac gatgccctct cacaaaatat ttcaggtgct attgttcgtg
      181 ttcacagtgg tgctcaaaca acaaaagaaa atgatgcttc atttatcaat ggaatagaca
      241 aggcctataa gagtcatatc actgaacttc aaaagcggaa tgtcccagtt gctgtctacg
      301 cttatgtagc tggaaaaagt gtccaagaaa tggaaaaggc tgccgaagtt ttctacaatg
      361 ctgcctctcc ttacagccct agttactatt ggcttgacgt ggaagacaaa accatgtcta
      421 atatgaacga aggtgtcgaa aactttcgag caaaactagc atcactaggt gctaaaaaca
      481 tcggcatcta cgttggtgtc tacttcatgg aagaacacag tattgataca ggcaagttta
      541 cctctgtttg gattccgtcc tacggctcag actcaggatt tttggagagc agtcctaaaa
      601 ctgatttaga ttatgatatt caccaataca catctaaagg aaaaattgcc ggctttgacc
      661 acgatttaga tatcaacgtt atctctccct taaaaaataa agaagaaacc tttcgaaaac
      721 tttttttaaa accttaaaag catttgttta tcatttgctt tttctttttg tgtctgattg
      781 tgatacaata tagtaaggat tttttgaaat agaaatagtt ggaggaaata tatgctctca
      841 tggttagcac gcgttattaa agggattgta attgctcttg gatttatcct accgggaatt
      901 tctggagggg ttctagcagc aatcttagga atctatgaac gaatgattgg ctttctggcc
      961 catcccttta aagactttaa agaaaatgtt ttgtacttta ttccagttgc catcggtatg
     1021 cttctgggaa tcggcttatt ttcctacccg attgaatacc tgcttgaaaa ttatcaggtt
     1081 tttgtattat ggagctttgc gggagctatt atcggtacag ttcctagcct cctcaaagaa
     1141 tcaactcgag aatctgaccg agacaagatt gatttagctt ggttatggac aacctttatc
     1201 atttctggat taggactcta tgccttaaat tttgtcgttg gaaccttaag cgccagcttt
     1261 cttaacttcg tcctagcagg cgcactattg gcccttggcg tcttggttcc tggcctcagc
     1321 ccatcaaatt tacttttgat tttgggactc tatgctccta tgttgactgg ttttaaaact
     1381 tttgatttct tgggaacctt ctttccgatt ggaattggtg caggtgcaac tctcatcgtt
     1441 ttttcaaaat tgatggatta tgccttaaac aactaccact cacgcgtcta tcatttcatc
     1501 atcggtatcg tcctatcaag tacccttttg atcttaattc caaatgcagg aaacgctgaa
     1561 agtatccaat acacaggact ttcacttgtc ggttatgtca tcatcgcctt cttctttgcg
     1621 ctgggaatct ggcttggtat ttggatgagt caattggagg ataaatataa ataatggcaa
     1681 aaaaagttaa aatcaaaaaa acattggtgg aacaaatcct atctaaagca gctatccctc
     1741 atcaggggat tcaaatcaat gccctagaag gagagcttcc tcaaggttat gaacgagatc
     1801 agattttcaa aaccttggcg cttttgggag acaagaccgg accgattatc ggaattgtcc
     1861 ctatcactca gcacttgtct gaaaagaaac tagctaaaat ttctggcaat aaaaaagtga
     1921 gcatgattcc acaaaaggac ttagaaaaaa caactggtta cattcatgga gccaataatc
     1981 ctgtcggaat tcgtcagaaa cacaattatc ccatttttat cgataaaatt gctctagatt
     2041 tggatcgaat gattgtctct gcaggcgaag tcggacacag cattatcgtc gcgccacaag
     2101 acttggctag ctttgtaaag gcagactttg tagatatttt ggaggacatc aagtaatgaa
     2161 actctacttt gtccgccacg gtcgtaccct ctggaaccaa gaaggtcgtt ttcaaggtgc
     2221 tagcggtgat tctcctcttc ttcctggatc cattgaaacc ctaaaacgac ttggccagta
     2281 tctcaaggaa attccttttg atcagattta ttcaagtgat ttacctcgag cggtcaaatc
     2341 tgctgagatt atccaaagtc aactccatac cccctgttct ttagaaatcg ttcctaatct
     2401 ccgtgaatgg caacttggga agttagaagg tttgaaaatt gcaaccctgg aagctattta
     2461 cccgcaacaa atccaggctt ttcgttcaaa tcttgctcaa tttgacactc gaatgttcgg
     2521 agccgaatcc ctctatagta caactcagcg gaccatccaa tttatcaaat cattaaaaga
     2581 tagtccagct gagcgtattc taattgtcgg acacggcgcc aatcttactg ccagtcttcg
     2641 tactctccta ggctataaag aaccacttct tcgtaaagat ggcggtctag caaatgccag
     2701 cctgaccatt atagaaaccc atgattttga aacattcact ctcaatactt ggaatgatac
     2761 ttcttatcaa tcataattat agcctagcct tctgaactag aaccatagga gatatagtca
     2821 gttgaaacaa aaataagaca aaagaacctt gtaaaaaggt attgcaactt gccaatacct
     2881 ttttgatatg aacacgtgtc ttctcaacag gattgtagag caaaatgaaa taagaactga
     2941 ataatatgat ttgagaattt aaatcaattt ataagaat
//
LOCUS       DAVMEF010000171         2974 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVMEF010000171 DAVMEF010000000
VERSION     DAVMEF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.171"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            152..499
                     /locus_tag="VYQ30_001816"
     CDS             152..499
                     /locus_tag="VYQ30_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1966875.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(779..1615)
                     /locus_tag="VYQ30_001817"
     CDS             complement(779..1615)
                     /locus_tag="VYQ30_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966876.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKLSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1628..2257)
                     /locus_tag="VYQ30_001818"
     CDS             complement(1628..2257)
                     /locus_tag="VYQ30_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1966877.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(2267..2908)
                     /locus_tag="VYQ30_001819"
     CDS             complement(2267..2908)
                     /locus_tag="VYQ30_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1966878.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
BASE COUNT          950 a          665 c          484 g          875 t
ORIGIN      
        1 ctccataata tctatagggg atttacccac tacaaatatt atagagccta aaagatgaga
       61 ccaaccaacc tcatcttttt tcgtatcaaa tctaagcaat tttagaaact ttatggcata
      121 ctagacttat caatgaaagc gaggtaatct catggaacaa tttttagata acatcaaaga
      181 ccttgaagtc actacagttg tgcgtgcgca agaagctctt gataaaaaag aaactgcaac
      241 cttctttatc ggtcgcaaaa cttgccctta ctgccgtaaa tttgcagata cattgtcagg
      301 tgtcgtagct gaaaccaaag ctcacattta cttcatcaat agtgaagaac caagccaact
      361 caatgatttg caagcattcc gctcacgcta tggaatccca actgtaccag gttttattca
      421 tattacagat ggacaaatca acgtccgttg cgactcttca atgtcagcac aagaaatcaa
      481 agatttcgca ggattgtaaa aaatcagcta acaagtctta aaatcacaaa aacgcataat
      541 gtaaggtgca aaaaccttga tattatgcgt tttattgttc ctaaaacagt tacatcagca
      601 tttcattaca gaacatttta attttggaag tcaaatcaat ttttaacaat ggtttagaaa
      661 tcacagtggg ctattccata ttcaattatc cagattcaat cgctattatc gttttcctag
      721 attacaaaaa aaaactacgt tttgctaatc aaaacgcagt cttagccact attttatctt
      781 actgtccttc ttttacttct ttggttgcta catcttctcc aaaccatttt tggctgattt
      841 cttggaactt gccgtccttg taaagactag aaaaagcttc atttatcttc ttaaccaagg
      901 ttgtatcttc cttacgggct ccaaccgcaa aagcttctgt ttctagtcca actgtaaaga
      961 cattataatc gtttaaaaca ccttctgctt ctaaataata gtttgcatag acacggtcaa
     1021 tcaatagacc atcaattcga tcgtttttca aatcaatcaa ggcttcatta aaggtttggt
     1081 attgattcgc ttccttatta gcgacaatat tcttcaaaag ttctggattt gcttcaaagt
     1141 ccgcataacc agatgaacca gcttgagctc ctaatgtctt tccagtcata tcctttgcag
     1201 tcgtgatacc agataatttc ttcgtaacca atacctgctc attcttcata tatgagttac
     1261 tgaaagccac cttttcacgg cgttcgtctg tagcggaata gccattccaa atcagatcaa
     1321 tcgttccttt tgtcaattca gcttctttca aatcccaatc aatcggttgc caatttaccg
     1381 tgattccgta tttttcaaaa acagctgtag ctaaatcaat atcaaatcct gcataagaac
     1441 catctttctg agcaaatccc attggaacaa aagtactatc aaatccaata gtaatagact
     1501 tgttagactg gtactttgac caattatctc cactagtttc gctagaattt ttcccacaag
     1561 ctactaagaa caaagcagtc atcagactga ctaatacaag catccatttt ttcatccatt
     1621 tttcctccta tttaggttct actttcaata atacatctgc gatattttca gcaaactgca
     1681 aatcatgggt aaccacaatc tgggtcatcc caagttccct attttgcaag attagcttct
     1741 ccacttccaa acgtaattct ggatccaggg cagaagttgg ttcatcgtag ccaatgattt
     1801 ctgggtcaat catcatagca cgcgccaaag ccactcgctg cttttgccca ccagatagtg
     1861 agaaaggata ggcctctgcg tgtcctccta gtcctaactg ttccaagagt ccactcgcct
     1921 tcttctcagc ctcttcctgc ttcattccca tggtcttcac aggcgataaa gtcaaatttt
     1981 ccagaactga tagatgagga aatagttgaa aatcttggaa gacaaatccc agtaggttgc
     2041 gcttctgcaa ttcatccagc tctaaaggtt gtccattata aaagatttgc cctgaatcaa
     2101 tggtttcaag acctgcaagc atacgtaaaa gagttgtctt acctccacca gaaggtccaa
     2161 cgatagccag gatttgcttt tcaggaatac ttagactgaa attagacagg atttgtttgt
     2221 ctccaaagac tttattgata tttcgtaatt ctaacatggc agcctcctat ctataataac
     2281 tatacttctt ctcaactttt ttggaaataa ttgtcacaat cccaatcaaa atcaaataaa
     2341 tggctcctgc caagaacata ggaaccagac tagcatcgcg gttagcagct gttcgactag
     2401 ccaagataag gtctgaaatt ccgagagcat agaccaaaga agtatccttg accaaactca
     2461 taacttcatt aaagacacta ggaagaacga tcttggtcac ttggggcaag ataatatagc
     2521 gcactctgtc aaaagggcta aacttcaaga ccttggcagc ctcatactgt cctcttggaa
     2581 tagtgtcaat tcccccacgg aaaatttctg caaagtaagc cgcataattc aaaacaaagg
     2641 caataatagc tgcaggaagg cggtctaaac gaatcccaat acttgggagc acataataga
     2701 taaaaatcag ttgcaagagt aagggggttc ctcgcataac ccagatgtaa atgttaatca
     2761 gataatggag gggcttccaa tggacttgca aggcaaaggc aatcaaaacg cccaagggaa
     2821 tcgaaaatag caagaccagt gcaaagacct gtacagtcgt gctcgcacca ttcagtaaac
     2881 tcggtaatat ctcaaataaa taagacatac tgcacctcct aaaagtaatt gttcctatta
     2941 tagcatgaat aaacaagatt accaagtatt tttg
//
LOCUS       DAVMEF010000172         2905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVMEF010000172 DAVMEF010000000
VERSION     DAVMEF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.172"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1077..1802
                     /locus_tag="VYQ30_001820"
                     /pseudo
     CDS             1077..1802
                     /locus_tag="VYQ30_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          548 a          670 c          965 g          722 t
ORIGIN      
        1 aagtcgacgc actggttgag gccgattcag atgctgacgt acttgcactt gttgacgccg
       61 atgtacttgc tgacgtgctt gctgacgtac ttgcacttgt tgaagccgat gtacttgctg
      121 aggtcgacgc gcttgttgat gcggacgtac ttgcacttgt cgatgctgac gtgcttgctg
      181 aggtcgacgc gcttgttgat gcggacgtgc ttgctgaggt cgacgcgctt gttgatgcgg
      241 attcagaggc actggtactt gctgaggatg acgcactggt acttgctgaa gctgaggcgc
      301 tagtacttgc tgaagccgag gcgctggtgc ttgctgaggc tgacgcactc gttgaggccg
      361 aagctgacgc actcgttgat gctgaagccg aggcactggt tgacgctgaa gctgaggcgc
      421 tagtacttgc tgaagccgag gcgctggtgc ttgctgaggc tgacgcactc gttgaggccg
      481 aagctgacgc actcgttgat gctgaggccg aggcgctggt tgatgcagaa gctgaggcac
      541 tggtacttgc tgaagctgag gcactggttg atgccgattc agaagctgag gtactggtgc
      601 ttgctgaagc tgatgcactc gttgatgccg attcagacgc tgaggtgctt gctgaagctg
      661 atgcacttgt tgatgctgag gctgaggcgc tggttgatgc cgattcagac gctgagatac
      721 ttgctgaggc tgaggcgctt gtacttgctg aagtcgacgc actggttgag gccgattcag
      781 atgctgacgt acttgcactt gttgacgccg atgtgcttgc acttgtcgat gctgaagcac
      841 tcgttgatgc tgaagctgag gcgctcgttg atgcggatgc tgacgcactg gtgcttgctg
      901 aagctgatgc actcgttgat gcggatgctg acgcactggt tgatgcagat tcagacgcac
      961 tcgttgatgc ggaagctgac gcactggttg acgctgaggc tgaagcactc gttgatgctg
     1021 aggctgaagc actggttgag gccgattcag acgctgaggt acttgctaca gatgacttgg
     1081 aaacttctgt acctgtattt ggcaattgct gtcttgactt acctgctgaa gccgaggcgc
     1141 tggtgcttgc tgaggctgag gcgctggtac ttgctgaagc cgaggcgctt gttgatgctg
     1201 aagccgacgc actcgttgat gcggatgctg aggcactggt acttgctgaa gccgaagcac
     1261 tggtacttgc tgaagccgaa gcactggttg acgctgaagc tgaggcgctc gttgatgccg
     1321 attcagacgc tgaggtgctt gctgaggctg aggcgcttgt gcttgctgaa gccgacgcac
     1381 tggttgatgc tgaagctgaa gcgcttgtcg atgctgaagc cgacgcactc gttgatgctg
     1441 aagctgaagc actggtgctt gctgacgtac ttgcgcttgt gcttgctgaa gccgacgcac
     1501 tcgttgatgc tgaagctgaa gcgcttgtcg atgctgaggc tgacgcactt gtcgacgctg
     1561 aagctgaagc actggttgac gctgaagctg atgcactcgt tgacgctgaa gctgacgcgc
     1621 tggttgaggc tgaggctgag gcgctagtac ttgctgactc tgatgcactg gtacttgctg
     1681 aggctgaggc acttgtgctt gctgaagcgg acgcactcgt tgatgctgaa gctgaagcac
     1741 tggtacttgc tgaggctgac gcacttgtgc ttgctgaagc cgacgcactc gttgatgcgg
     1801 aagctgaagc actggtactt gctgaggctg aggcgctagt acttgctgaa gccgacgcac
     1861 tggtacttgc tgagactgaa gcacttgtgc ttgctgaagc tgaggcgctt gtgcttgctg
     1921 aagccgacgc actggttgat gctgaagctg acgcacttgt tgatgctgag gctgaggcgc
     1981 ttgtgcttgc tgagactgaa gcactcgttg acgctgaggc cgaagcactg gttgaggccg
     2041 attcagatgc tgatgtgctt gctgaagccg aagcacttgt cgacgctgaa gccgacgcac
     2101 tggttgacgc tgaagctgac gcactggttg acgctgaagc tgacgcactg gttgatgcag
     2161 attcagaagc tgatgtgctt gctgaggctg acgcactcgt tgatgctgac tcagacgcac
     2221 tcgttgacgc tgaagccgaa gcacttgttg acgctgaggc tgaggcgcta gtacttgctg
     2281 aagctgatgc actagtactt gctgaagcgg acgcactcgt tgatgcggaa gctgacgcac
     2341 ttgtacttgc tgaggctgag gcacttgttg atgccgaagc cgaagcactg gtacttgctg
     2401 aagccgatgc acttgttgat gcggattcag aggcactggt tgacgctgag atacttgctg
     2461 aggccgacgc acttgttgat gctgaagctg aggcgcttgt gcttgctgag gctgacgcac
     2521 ttgtcgacgc tgaagctgaa gcacttgtcg acgctgaagc tgaagcgctt gtcgatgctg
     2581 aggctgaagc actggttgat gccgattcag aggcacttgt tgacgctgag gccgaggcgc
     2641 ttgtcgatgc tgaggccgaa gcactggtac ttgctgaagc cgaagcactc gttgacgctg
     2701 aagctgaggc actggttgat gctgaagccg aggcgctggt tgacgctgat gctgaggcgc
     2761 tagtacttgc tgaggctgac gcacttgttg atgctgaagc tgaggcactg gttgatgctg
     2821 aagccgaggc actggtgctt gctgattcag atgcacttgt cgatgctgaa gccgaggcac
     2881 ttgtcgacgc tgaggctgag gcact
//
LOCUS       DAVMEF010000173         2901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVMEF010000173 DAVMEF010000000
VERSION     DAVMEF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.173"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            188..2203
                     /locus_tag="VYQ30_001821"
     CDS             188..2203
                     /locus_tag="VYQ30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1966879.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWFIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGEGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2205..2837
                     /locus_tag="VYQ30_001822"
     CDS             2205..2837
                     /locus_tag="VYQ30_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966880.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWKMADEVFTMDHLK"
BASE COUNT          869 a          535 c          610 g          887 t
ORIGIN      
        1 aaaacaagga aaaagaaagg ggtttcattg catgagaaaa aaacgtggaa ttaaaaaatt
       61 agttacattt gatgcgggat ggtaaagaat aattgaagaa gattctttta tcaatagttt
      121 agtaagcttg ttagtttata gagggcaggg gtatttctgc cctctgtttt agaataagga
      181 atgcactatg aaaaaattat ttatactcat atccaacctg cttgctagtt tgttctttgt
      241 ttgggtattt accatttgga ctgatacata tgtctcccat tattacccca acgttgttgt
      301 acgtgattct tcccctgaaa caacttttca gcatgttgct acacgcttgg aaaaactagc
      361 agaagaaaca gatagcttta ttgcgattca acaccaagat cctaactcag aaggtactac
      421 agttttttct tatacgacct ttggggacgg aaagttgcct gatgggcttc aggaaaaaaa
      481 tctagaagat gctcaaagta gtagtgttga aacaaactat tttgtattcg atggacacct
      541 agatattcac ttgctaaggg aagagctaag tcaacttggc ttgactaaca tgcacctgac
      601 aattccatct aaactatcta cgttaatggc tatctttagt aacggatttc agttaatcag
      661 tctattgatt ttcatcttaa cctttgtagc actgacttta attagtcaaa ttagccaatt
      721 acggtcatcg ggcattcgcc tcatttcagg agagaaacgg tggttcattt tccttagacc
      781 agttggtgag gatttgaaag cgatagctgt tggtttcagt ttagctggcg tgctcgccat
      841 tcttatgcaa aaaattctgt cgcttccaac gcaatcccta atgaccatag gagaaggctt
      901 gctcagttat aatctcatct tgttgtcgat ctccctgttt ttcgctcaac tcttcgcagt
      961 tgggataaaa aagatacacc tgatgcagat tataaaaggg caagtgcctg tcagaggaat
     1021 cattagtttg attttaatcg gtcaactatt agcgattatt attgtaacgc tgggaatagg
     1081 gagtagctta aagtattccc aagcctggca acagcatcgg attggacaag aggcttggag
     1141 tcaggaaagg caactgatta ccctatcaat cagccgtgaa ggaacgagtc ctggttttga
     1201 tgaacaagct caaaggaaac tcagaacttg gtatcaattg atggatctgg ctgtttcaga
     1261 acaaaaggct ttcttatcta gacaccagtt aattgaccgt actttgcaaa atggcatggc
     1321 ttcctccaaa aacttgataa cctctacaga atggcacgat tacaacccga atggcaatgt
     1381 ccttatcgtc acaccgcaat acttggagcg tcaaaacatt cctgtagata caactattga
     1441 gcaaaagatg aatcaccttg atgtagggga gtttgtctta ttgctgcctg aacacctccg
     1501 ttcagaggaa gaacattata aatctgtttt tgaagacgac ttaaccagtc gcatgtctag
     1561 tcaagatgaa cgacagcaaa tgactgctac ggtaggttat ttagaatcag gtcaggatcg
     1621 ttttgtgtat aatacgaccc ctatttctta ccagcagttt ttgaaagatc caatcatcat
     1681 tgttataaca ccccaatcaa ctggtccaca gtccattttg ttttggatag acgcagtaca
     1741 gaactacgtt ctctttaatc aattgtctga tgcccaggag cttatccaga gacaaggcat
     1801 tgaaaattgg gtctcagaaa tgcaaacagg ttaccacaac tacatcacat tattggataa
     1861 tatccagagg gaacgttggg taatgctagc aggagctgtg cttgggattg caacttcaat
     1921 cttgttgttt aacactatga ataggctcta ctttgaagaa tttagacgtg ccatttttat
     1981 caaacgcatt gcaggtctca ggttcttaga aatccatcgc acttatctct ttgctcaact
     2041 gggtgtgttt ttactgggat ttgttgcgag tgtatttctt caggtagaga taggagttgc
     2101 tttcttagtc ttgttactct ttactggtct atctctttta cagttacatg tccaaatgca
     2161 gaaagaaaac aagatgtcca ttcttgtttt gaagggaggt taatatgatt gaacttaaac
     2221 aggtgagtaa atcttttgga gaacgagagt tattttcgaa tctttcaatg acatttgagg
     2281 ctggaaaagt ctatgcctta attggttcaa gtggtagcgg aaaaacaacc ttgatgaaca
     2341 tgattgggaa attagaacct tatgatggga cgatttttta ccgaggtaaa gacttggcca
     2401 attataaatc gagtgatttt ttccgtcacg aattgggcta cctcttccag aactttggct
     2461 taattgaaaa ccaaagtatt gaagaaaacc ttaagctagg tctcattggt caaaagttga
     2521 gtcggtcgga acagcggttg aggcagaagc aggctttaga acaggtcggc ctggcttatc
     2581 ttgacctaga taagcgcatc tttgagttat cgggcggaga atcgcaacgg gttgccttgg
     2641 caaaaattat cttaaagaat ccacccttta ttctggcaga tgagccaaca gcttcaatag
     2701 acccagcaac ctctcagttg attatggaga ttttgctatc tcttcgagat gataataggc
     2761 taatcattat cgcaacacat aatccggcaa tttggaagat ggctgatgaa gtgttcacga
     2821 tggatcatct gaaataaaaa tccttgtttt taattgcacg atgagttact gaaatattat
     2881 catgaatcaa gaattggagt t
//
LOCUS       DAVMEF010000174         2896 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVMEF010000174 DAVMEF010000000
VERSION     DAVMEF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.174"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1342)
                     /gene="rlmD"
                     /locus_tag="VYQ30_001823"
     CDS             complement(<1..1342)
                     /gene="rlmD"
                     /locus_tag="VYQ30_001823"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1966881.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKL"
     gene            1380..2156
                     /gene="recX"
                     /locus_tag="VYQ30_001824"
     CDS             1380..2156
                     /gene="recX"
                     /locus_tag="VYQ30_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1966882.1"
                     /translation="MKITKLEKKKRLYLMELDNVDKCYITEDTIVRFMLSRDKLISEE
                     ELKEIQNFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2245..2778
                     /locus_tag="VYQ30_001825"
     CDS             2245..2778
                     /locus_tag="VYQ30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1966883.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          929 a          534 c          528 g          905 t
ORIGIN      
        1 ttaattttac aacagcttca gttcgagctg tatgtgggaa catatcgacc gactggatat
       61 aatgaagatc atagacttct actaagcgta ccaaatcacg agccaaggtc gaaacattac
      121 aagaaatata aaccattttt tctggtacat aagtaagaat agtatctaat aacttatcat
      181 ccagacctgt acgtggtggg tcaacaatca aagcatctgc tcggtagcct tccttgtacc
      241 aacgaggaat aatctcttct gccgttccag cttcataatg agtattgtca aatcccattc
      301 ttttagcatt tcgcttggca tcttcaatag cttctggaat aatatccata cctctgagtg
      361 tttttacttt ctttgcaaag gcaaatccaa tcgttccaac tccacaataa gcgtcaatca
      421 aatggtcttc tttatcaaca tccagcgctt ttactgcttc gctatagagg acttctgttt
      481 gctcaggatt tagttgataa aaagctcgag gggatagtga aaattcataa ttgagtacac
      541 cttcttgaat actctcttgc ccccagataa tctctgtctt ttcaccatat atctcactgg
      601 ttttagctgt atttgtatta acagctactg tcacaacttc tgggaaatct ttaaccaact
      661 cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac aataataatc tgaacctgtc
      721 cggtctttct cgcgcgtcgg accataatag tacggacacc tagaactttt ctctcatccg
      781 tgattggaat ctggtgataa gtaagtaatt ctgctaagcg attagcaatc acttgggttt
      841 ccttatcttg taccaggcag tctttcaact ctactaaata gtgagagttt tgtgcatata
      901 agcccgcctt gacctgattt ttaaattttc gagtctgaaa ttgtaactta gctctgtaat
      961 attttggttc ctgcattcca atagttggac gaatttcata attttcatat cctgcaggag
     1021 caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat
     1081 gcaggtgcat gatttggcag cctccgcatt cattataaat agtacaagat ggcacaattc
     1141 gaaatttaga cttcttgttg accttcagta attttgcttc aacaaagttg cgtctaatag
     1201 aagtaatctg acaatagata tcttcgcctt tgagagctcc tggtacaaag actaatgttt
     1261 tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg cttgattttt aatggtattt
     1321 tttgtttcac tttcagattc atacccctat cttatcacat tttgagttat aatagagcta
     1381 tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct gatggagctt gataatgtcg
     1441 acaaatgcta tatcaccgaa gacacaattg ttcgttttat gttatcgaga gataagctga
     1501 taagcgaaga ggaattgaaa gagattcaga actttgctca attttcttat ggtaagaatc
     1561 tggccctcta ccacttatcc tttaaagcac gcactgaaaa agaagtcaga gaatatctga
     1621 aaaaatacga tattgatgaa aacatcgttt ctcaagttat tgctaatctt aaagaagata
     1681 agtggattaa tgatggtcag tacgcttatg ctatcatcaa taccaatcaa ctttcaggag
     1741 acaagggtcc ttatgtgctg actcagaaac tagctcaaaa agggatttca aaatctacta
     1801 tagaagagaa cttgaaagaa tttgattttt ctgaagttgc tcaacgtgta gctaataaac
     1861 tattgaaaaa atatgaggga aaacttccag ctcgtgccct gcaagataag attatccaga
     1921 acttgactaa caagggcttc tcttattctg atgctaaaat tgcctttgac gacttagata
     1981 gccaagttga ccaagaaacg actcaagaac tcatcttcaa ggaacttgat aagcaatata
     2041 ctaagtatgc tcgaaagtat gaaggatacg aactgaagca gcgtttaact caagttttag
     2101 cacgaaaggg ctacgatttt tcggatatag caagcgctct tagagaatat ctttaatatt
     2161 tccaagtgaa attcacagat tttaggtaat tttatggtac aatagtaaat gataaactta
     2221 taaattgtag aaagttggtt agttatgaag cttccaaaag aaggcgactt tattacaatt
     2281 caaagttata agcatgatgg gagtctccac cgaacttggc gggacaccat ggtactaaaa
     2341 acaacagaaa acgccattat tggtgtcaac gatcatacac ttgttaccga aagtgatggt
     2401 cgtcgttggg tcactcgaga accggctatt gtttactttc acaagaaata ttggtttaat
     2461 atcattgcca tgattcgtga taatggaact tcttactatt gcaatatggc tagcccctac
     2521 tatctggatg aagaagcact gaagtatatt gattacgatt tggatgttaa aatttttaca
     2581 gatggggaaa aacgtctctt ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat
     2641 tattctgatg acttggacta tattttaaaa gaacatgtca aaattcttgt tgattggatt
     2701 aacaatggac gtggtccttt ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta
     2761 gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt
     2821 agtataaaat aaattgtgta aacacaaaaa ggaataaatc cattatagca gagttacaac
     2881 acattactag aaagag
//
LOCUS       DAVMEF010000175         2882 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVMEF010000175 DAVMEF010000000
VERSION     DAVMEF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.175"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            114..791
                     /locus_tag="VYQ30_001826"
     CDS             114..791
                     /locus_tag="VYQ30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966884.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMAALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            803..1468
                     /locus_tag="VYQ30_001827"
     CDS             803..1468
                     /locus_tag="VYQ30_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1966885.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSALVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIVGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            1540..2766
                     /locus_tag="VYQ30_001828"
     CDS             1540..2766
                     /locus_tag="VYQ30_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1966886.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT          727 a          515 c          549 g         1091 t
ORIGIN      
        1 tctatacttt tagaagagta cagactacaa gctgagagaa ttttgaaatt ttaactttat
       61 tcctccagtc cttgcaatta gatataaaat aggctcttat aggagataat aggatgaaga
      121 aaatgaaaga agtgaaattt cacctagcaa ctggcttgtt gattttgact tattacctca
      181 tttttaatgt cacatcagac ttagatttta tggctgcttt gtctgataat atgtactatg
      241 tgttccaagt tttattggta cttattctgg gaactatagc tacaattgct tttgtcaaaa
      301 gtgaacattg gaaagagtgt ggtcgtttcc agtttcgttg gtcttacctg ggtgttttcc
      361 ttctttcttt tttcttattg ttcgtgtggg caaatctaac aacctatatc tttcctcgta
      421 cccagaatgg aagtacagtc gtagaagtag ccaccaattt aacaggaatt agctattttg
      481 ttacgcgaat actttatact agtattatcg cacctgtatc agaagaggtt gtttgtcgtg
      541 gtcttttgat gacaagttta tctaaagtta agagatacta tcttgatgtt cttgtttccg
      601 ctgccatatt tggcgctatg catgttttgc agtatggttg gataacgaca gatttcataa
      661 agtattttgg aatgggcttg atattttgta tgatgtttcg ctacacccga tccatctatt
      721 gggctatagc tctgcatgct tcatggaata gtttcttact cattgttact ttattggtat
      781 ttggatatta gaaaggtgat atatgaaaaa gataatctca caccgctact ttattatagt
      841 ttttctacta gttattgctg accagaagtt cagtgctcta gttttacgca gtgaccttgt
      901 tactgggcta tctgactttg cctattatct gtcggatatg atgttgaatt ttcttgtggt
      961 tttatttgct cttattgcta tgatttggtc ggggaaatgg cagaaaatca atagtagaaa
     1021 gtttaaagga tcctatcttt tctattcctt cctagctctg cttgcttttg ttgtttggaa
     1081 tttcgttacc ttttttcttt tcccacctac tcgaaatgaa atttcttatc aacatgctgc
     1141 tccgactttt acaggggcta cagcattttt gatgtatttt ttctatcctg tgattgtagg
     1201 tcccattttt gaagagataa tccatcgggg attggtgatg acagctctgg aaaaaggaaa
     1261 gaaatggggg ctagatgtac ttggttccgc tgttttattt ggggtctcgc acattagtaa
     1321 tcacggttgg gtcttgacag actttttcgt ctatatgggt ggcggactta tatttgcagt
     1381 cttatttaga atgacaaagt ccatttattg gcctattggg ctgcatatag tctacaacgg
     1441 tattggtcag cttttgatgt tactgtaatt ttggttgtta gtagtatcgc ggtctatttc
     1501 tggggaaaga ttcccatttg aacttaagaa ggagttgtga tgaaagtatt tcttcaaaat
     1561 agagatttta ggcaattaac tatcaaccag tggatttcaa cgcttgggga tacgattttt
     1621 tatctggcct tcctgaatta tgtggcagat gcgtcttttg cccctttggc gattttactt
     1681 atcacgattt cagaaaccct tccccaagtt ctacaaatct ttctgggagt tttggcggat
     1741 tttcaacatc atcgtgtctt gaaatacaca gtcattagtt ttacaaaatt tttgctttac
     1801 tctatagtgt ccctatcgct ttcagggcag tctttttcct tgttgctagt aacctttatt
     1861 tgtctgctta acctcttgtc tgacacattg agttattttt caggcgccat gctcactccg
     1921 attttcatta gaattattgg gcaagaccat ctggcagaag ctattggatt taaacagtca
     1981 actgttagtt tagtgaaaac aatcagtaat atcctaggag gagtcttact aggcattcta
     2041 tccatccagt ttatttcctt actgaacgct cttacctttt taatcgcatt tttaggtatc
     2101 ctcttcataa aaactgacct cttgaaagta gaaaaaacga ttagctatca agaaggactc
     2161 tctgtaaaat ccttttgcca gcatttgctc caatcatcga aattgatatg gaatatgaat
     2221 aaggtgctct tggttttgtt tattatctct actagtcaag cagtgataaa tgttacggtt
     2281 cctatttcca ctctattttt aaggaaccag ccctttttga atttacaaac tggtcaatct
     2341 cttgccttgt tagccacact agaattatca gcccttattt tgggaagtct tatgagtggc
     2401 tatctgcaac ataccatttc cataaaaaca gctctctatg cctcacttgt tatccagttg
     2461 cttcttctag taggatttgc cacagttcgt tttgactgga ttctcatctt tagtaccttg
     2521 gatgcctttt tcgcgggtat tctctctcct agattgcaag aactcgtctt taaacaaata
     2581 cctgaggagt caatgggagc agttcaatct tctatcggtg ccattacggt tgtcttacct
     2641 agcttattta caatagcttt ggtaaccatt gctaccagct ttggaactct ggcagttagc
     2701 tttgttttat tgctatttct tctagttgcc tttgtcatgc ttctgaatat tcgtgaaagt
     2761 atttagtgga gaatttctat aaaatatgtt tgagactgga ttgccagtct ttttatgatg
     2821 gtcaaaatca acagactttt ttctcttttc tgaatgtgtt atagtagact gaatctaaaa
     2881 ta
//
LOCUS       DAVMEF010000176         2828 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVMEF010000176 DAVMEF010000000
VERSION     DAVMEF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.176"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(97..384)
                     /locus_tag="VYQ30_001829"
     CDS             complement(97..384)
                     /locus_tag="VYQ30_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966887.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(412..1044)
                     /locus_tag="VYQ30_001830"
     CDS             complement(412..1044)
                     /locus_tag="VYQ30_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1966888.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(1048..1689)
                     /locus_tag="VYQ30_001831"
     CDS             complement(1048..1689)
                     /locus_tag="VYQ30_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966889.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(1682..2473)
                     /locus_tag="VYQ30_001832"
     CDS             complement(1682..2473)
                     /locus_tag="VYQ30_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966890.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(2495..>2828)
                     /locus_tag="VYQ30_001833"
     CDS             complement(2495..>2828)
                     /locus_tag="VYQ30_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966891.1"
                     /translation="TTFVEANQIGAFSNFVITTSYKRTGYLTKENEGAEYIMNLNPCR
                     NLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWATVGYVYAADMARQNWWDLSAA
                     ISGSWSPN"
BASE COUNT         1043 a          536 c          436 g          813 t
ORIGIN      
        1 tagcacaatt actgattatt ttcaaggaag aatccccatt ttgaaaagga aaaagaggtt
       61 agttttcaag ctaacctctc cgacttttat gactgtttaa ttgccaaaaa tcggcaaaat
      121 ggcaaatgtg ataaataagt taatccaaag agaaagacag caaatcagtc caaaaacaaa
      181 gagactgact ttcttggaca gcaaggccat gagaatggac agaattccaa aagctagcaa
      241 gattttctgt atgatgaaga tgtcaatcct tacccccaga atcgctctat cccaagggat
      301 aaagaagaaa gacagccaga tcagcagaac taaaccaata taaaccttgg aatagcgtgt
      361 aataaatgat tttagatgtg ccataagcca tctcctagga ttgtatatcc tttacatttg
      421 tatatgtcca ttttcgacaa ctaatacatc tgtacaaagg tctgaaatat cttccgatat
      481 gtgcgacgtc aggataacca gaccattttc ttttttcagg taagataaaa tgacctgttt
      541 ggttaacctc acactcttct catccaaagc attcataggt tcatcgagaa agagtataga
      601 aggagaggaa ataaaggctt gaatcaaggc cattttttgc tttgttccca aggataaatg
      661 acggtattca acgtcttcaa attcctgtaa atcatagtat tgaatccaat aggcaattct
      721 tttttccgta actttagatg aaaaatacct taacagttcc aaattttctc tcagggataa
      781 atgagataaa aactcgactt tttcaattaa aattcctgca tcctgaatat aatgattttt
      841 tatcccgtag actttcccgt cttgaagaac ttttcctttg tcaagcttaa tatagccagc
      901 aagtaccttt aaaagaaccg tcttgccaga tccattatca cctttaagtc cataaatttt
      961 tcctgattga aacaccaaat tcaactgatt taaaataggt cttttcccat aaattttctg
     1021 tccatttata atttcaagtc tcatcgttta tcctttctta aatttctctt ccaagcgact
     1081 aacaatcaac ataactaata aacttaagag tagtaaatac actgttacaa tatgatccat
     1141 catccattct tgtcccacaa atactaaaaa atagagaaaa aaggaaaaac cagcgcccgc
     1201 actaatcagt gctatcagat aagacagtag taagtacaac atcattaact gagcaagtaa
     1261 aaagaaaagt gcgactgttt tgaaatgaat tcctagcgga agtaaaccaa ggaaccagac
     1321 aaacaaatct tgagtaaaaa atttagaaat gacaaccaat cgtttccttg tataatcgaa
     1381 aaaggagctt gattgatagc gaatcaagtg atggtaatgg tctgccaaat cactttcaag
     1441 taaaatgaca gagagccatc ctaccatgaa aagcgaaagg tttaacaaga gtttggggat
     1501 ttctaaattc gcttcagaat agtcattata ggaaaaaatt gaaatcaatt ctctcttgaa
     1561 aaagataaca gcatcctgct tttgaaccgc tgttgacaat agatggttta tcaaaatcat
     1621 catcagaact atacgaagta gtaaaacaat ctttctcttt agcaaaatat tgttaaaatt
     1681 cttaaacatt atcttcatat ttttcaagca ccatgtaagg gacaatataa aggatataag
     1741 gagtaaagag tttcatcaaa cttacatccc catagctagc ttgcatcaaa ctcgtcatag
     1801 gaaccatata gtaaggcaag gcactataga gcagcataga accaagccaa agaaacatca
     1861 aataggtgat tgccgaacga gtcacattcc ccacataatc aactatttgt aaaaaataga
     1921 ttgcattgat gaaaataccg attagcagga cacaagtaaa gaacaaatag ctttttatct
     1981 ctccatctag gagtctttgt aaaccacttc catcagaaaa tagagaattc catccaagag
     2041 gagaaaaagt cccaaggaaa taggttataa ttgcaattgt cagcacagtc actaaatata
     2101 tcaaacaagg gatacttgca acttgcaaag tcaactttct ttttaaccct ggatagctca
     2161 cttctcttcc aatactcaat cgtaaaactt tatttttcag ctcaatatat tgaaaaccta
     2221 gcaaaacaat gatgattgga ataataaatg attgataaaa acctagattg aactggagaa
     2281 tacgaaaatc taatgctgat tttaataagt catttttaat tgcttgtcca tcgaatttag
     2341 ctagatcggg cgcatgaata tcaaaaaaga aataagtcat caaaacatca cgataaacca
     2401 aagcaagaaa aagcaaagta agcaagacat aaacaaattt atgccttgcc aaagttaaga
     2461 gaggaatctt cataaattat acctacttta cgaattaatt tggtgaccaa ctacctgaaa
     2521 tggcagctga taaatcccac caattttgtc ttgccatatc tgctgcataa acatagccaa
     2581 ccgttgccca attaccatga gttgaacgag taccacaggt tgttaacgat tctccactac
     2641 gagcttctcc attagaattt actatacgat gcttaacagt catgggatgt aaatttctac
     2701 aaggatttaa gttcatgata tattccgcac cttcattttc tttagtcaaa taacctgttc
     2761 tcttataact ggtagtaata acaaaattac taaaagcacc tatctgattt gcttctacga
     2821 aagtagtt
//
LOCUS       DAVMEF010000177         2780 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVMEF010000177 DAVMEF010000000
VERSION     DAVMEF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.177"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(305..1006)
                     /gene="galE"
                     /locus_tag="VYQ30_001834"
     CDS             complement(305..1006)
                     /gene="galE"
                     /locus_tag="VYQ30_001834"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1966892.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTH
                     DGSCIRDYIYVLDLA"
     gene            1276..2646
                     /locus_tag="VYQ30_001835"
     CDS             1276..2646
                     /locus_tag="VYQ30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1966893.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISSQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT          726 a          544 c          542 g          968 t
ORIGIN      
        1 caacaagact ttctcctttg ttatcctatt ctaaaatgtt tttaccttaa tctgataaaa
       61 taatatcttc gagggagtag ctagccgtcc aatcaagata ttgtttagct tttgaagcat
      121 ctactaggac actggctggg tcactagcac gtcgagcaac aatctcgtgt gggatttttt
      181 aatttagtaa ttcttcagca gttttaaaga tttctttgat agtatagcct tttttagttc
      241 ctaagttaaa gatttgagaa gaactgtctt cttgaaatag gtagttcatt cctttaacat
      301 gagcctatgc aaggtccaag acataaatgt aatctcgaat acatgaaccg tcatgtgtat
      361 cgtagtcatc tccaaatatt tttaagctat cattttgtcc caatgcggtc ttgttgatat
      421 ttggaatgat gtgagttgga tttttcacac gcagaccgtt tgaagcatcc atttcagccc
      481 cagcaacatt aaagtaacgg aaaataacat atttccagtc gtagcgattg gccatccagt
      541 aaatcattcg ttcgcccatc agttttgtct ctgcataagg gttgacaggg tcgagcaggg
      601 tatcttcagt caccggcttg tcaatacagt tatttccata gagagaagca gtcgaagaga
      661 acatgatttt ttgaatgcca acttcagata agactttgag aacttggttc ataccagcaa
      721 cgttggcagt gaagtattta cttggatttt caatactttc gctcacaaca atttcacctg
      781 cacaatggag gacagcatca atctgatttt cttctaagta agcctttaag gcgctagcat
      841 cataaacatc cagttcttta aagctagcac ggctatccac agccgctcga tttcctgtgg
      901 agagattatc gaggacatgc acctgatagc cagcatttaa aagagcttta acggtatggg
      961 agccaatgta gctagtccca cctgtaacaa gaattgtttt ggtcatgatt ttttcctttt
     1021 ctagttttgt actatagtaa attgaaataa gatatgaaca actctattag gaaagtcaaa
     1081 ttaatttcta gaaatgtttt agcagctaca gcgtactatt ccaaactcaa ccaactataa
     1141 tagatcgaaa ctagaatagt acacatctac ttctaaaaca ttgttaaaaa tcgatttgac
     1201 tgtccttatt tcattccgct atatataagt ttgctgtttc ttgtcgctcc tctggaaagc
     1261 tgatataata gctttatgaa taaaaaacga acagtggacc tgatacatgg tccgattctt
     1321 ccctcgctct taagcttcac ctttccaatc ttgctatcaa atatttttca acagctctat
     1381 aacactgctg atgtcttgat tgttggacga tttcttggtc aagaatcctt agctgcagta
     1441 ggagcgacga cagcgatttt tgacctgatt gtaggtttta cacttggtgt tggcaatggc
     1501 atggggattg tcattgctcg ttattatggg gctcggaatt tcactaaaat caaggaagca
     1561 gtagcagcca cctggatttt aggtgctctt ttgagcattc tagttatgtt gctgggcttt
     1621 cttggcttgt atcctctctt gcaatactta gatactcctg cagaaattct tcctcaatct
     1681 tatcaatata tttctatgat tgtgacctgt gtaggtgtca gctttgctta taatcttttt
     1741 gcaggcttgt tgcggtctat tggtgacagt ctagcagccc tgggatttct gattttctct
     1801 gccttggtta atgtggttct ggatctctat tttattacgc aattgcatct gggagttcaa
     1861 tccgcaggac ttgctaccat tatttcgcaa ggtttatcag cggttctctg cttttattat
     1921 attcgtaaaa gtgtgccaga actcttgcca cggtttaaac atttcaaatg ggacaaaagc
     1981 ttgtacgcgg atctcttgga gcaaggtttg gctatgggct tgatgagttc aattgtatct
     2041 atcggcagtg tgattttaca gttttctgtt aatacatttg gtgcagtgat tattagttcc
     2101 cagacggcag ctcgacgcat tatgaccttt gcccttcttc ctatgaccgc tatttctgca
     2161 tcaatgacga cctttgcttc tcagaatcta ggagctaagc gacctgaccg cattgttcaa
     2221 ggtcttcgaa tcggcagtcg tttaagtata tcctgggcag tttttgtttg tattttcctc
     2281 ttttttgcca gtccagcctt ggtttccttc ttggctagtt cgacagatgg ttacttgata
     2341 gaaaatggaa gtctctatct gcaaatcagt tcagcctttt atcccatttt gagcctcttg
     2401 ttgatttatc gcaattgctt gcagggcttg gggcagaaga tccttcctct agtttctagc
     2461 tttattgaac taatcggaaa aatcgttttt gtggttttga ttattccttg ggcaggatat
     2521 aagggtgtta tcctttgtga acctcttatc tgggttgcca tgacagttca actgtacttc
     2581 tcactattcc gtcatccctt gataaaagaa ggcaaggcaa tcttggcaac caaagtgcaa
     2641 tcctagttgg atttactgaa taaaatccat ttcctctagt gaaaatcgaa aaaacttgtg
     2701 ttctcttctt tagtttggtg ttgaaaatag tttaacagac ttttgacttc ttttatatga
     2761 tataataaag tatagtattt
//
LOCUS       DAVMEF010000178         2773 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVMEF010000178 DAVMEF010000000
VERSION     DAVMEF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.178"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            217..1122
                     /locus_tag="VYQ30_001836"
     CDS             217..1122
                     /locus_tag="VYQ30_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1966894.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            1119..1934
                     /locus_tag="VYQ30_001837"
     CDS             1119..1934
                     /locus_tag="VYQ30_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1966895.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2064..2756)
                     /locus_tag="VYQ30_001838"
     CDS             complement(2064..2756)
                     /locus_tag="VYQ30_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1966896.1"
                     /translation="MGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELKSWFKNEIV
                     EEESICLIGHSLGGDLARYSASEFEEVKKLILLDGGYLDLDKILPLDTELEETKNYIK
                     SQIVSDLALLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYENIEAILRLR
                     RKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHELYTQAPKEI
                     ASLMNEWLAYFL"
BASE COUNT          788 a          589 c          481 g          915 t
ORIGIN      
        1 gctactcaat ttgaaatttt aacttttccc ttttccgcaa aataatagta taatagaggt
       61 agaatttaga atcgaggtac acctatggct gtcaaattta caaaacgaga cgacttggac
      121 aagatgtttg aagagtttgc taaactccct gatttgaaac aagttacttt ccctgatgac
      181 aaagagaaaa aagtcaaagc agaaaagaaa aactagatga ctgcttttca acaactccca
      241 tctagtgtac ttcaaactgg agccattttt ctctccatta tcattgaagc ccttcccttc
      301 gttctgatag gaagcattgt ctcagggctg attgaagttt atatcacacc tgacaaggtt
      361 tatcattttc tccctcgaaa tcgttggggg agaatctttt ttgggacctt tgtcggtata
      421 cttttccctt cttgtgaatg tggaatcgtc cccatcatca atcgttttct ggaaaaaaag
      481 gttccaagtt acacggccgt tccttttctt gtgacagcac ctgttatcaa tcccattgtt
      541 ctttttgcga cctattctgc ctttggcaac tccttccatg tcgccctatt acgagctctg
      601 ggttccattc ttgtggctgt aatactagga atttttctag gatttttctg gcaagaaccg
      661 attcagaaag aaaatcgtct ggcttgtcat gagcatgatt tttcttactt gagttctgca
      721 aaaaaagttt ttcaagtctt tgtgcaggcc attgatgaat tttttgatac ggggcgttat
      781 ttggtatttg gctgcctctt tgcttctata atacaagtct acgttccgac tcggattctg
      841 acctctatca gtgcgactcc tctttttgcc atcctgctct tgatgctttt ggcctttctt
      901 ctttcgctct gtagtgaggc ggatgccttt ataggtgctt ctctactctc gagtttcggt
      961 ttggctccag ttctggcctt tctcgtcatt ggcccaatgc tggatatcaa aaatattctc
     1021 atgatgaaaa attacttgaa agcacgattt attagtcact tcattacgat tgtgactctg
     1081 gtcgttttag cctattctct cttgattgga gtcatcctat gattcgattt ttagttttag
     1141 ctggctattt tgaactgact atttacctcc atctgtcggg caaactaaac cagtacatca
     1201 acatgcacta ttcctatctg gcctatattt ccatggtgct ttcctttatc ttggcaattg
     1261 ttcaattgta tatctggatg aagcaagtca aaacccacag tcatctgaac agccgattgg
     1321 ccaagatgac gagtatttct cttctggcta ttccacttgt catcggctta actttcccaa
     1381 ctgttagctt ggattctcag actgtttctg ctaaaggtta tcatttcccc ctatcggaag
     1441 gaacggatct agccattcag acaagcgaag ggacgacaag ccaatatttg aaaccagata
     1501 ccagttctta tttttcaaaa tcagcctatg aaaaagaaat gcgaacggcg gcggataaat
     1561 acttatccca agatagtatt cagatcacta atgaaaacta tatggaagtc atggaggcta
     1621 tctacgacta tccagatgag tttgagggca agacaatcca gtttacaggc tttgtctata
     1681 acgaccccag tcatgccaat agtcaatttc tgttccgatt cggcattatc cactgtatcg
     1741 cagattctgg tgtctatgga ttgctgacca agggcaatac ccggcagtat gaaaacaaca
     1801 cttggatttc agccaaagga aaactggtca atcactacca taaagaactc aaacaaaacc
     1861 ttccaacctt ggaaatcgac agctttacca aagtcgataa accagaaaat ccctatgtat
     1921 atagagcttt ttaagaaaat caagataaaa acgaacaagt tctcttctga ataacagaaa
     1981 aagagcctgt tcgttttttg gttatatgaa aattagtgac ttgtagattt tcatcttata
     2041 ccatttccag caatacaagt agctcataga aaataagcga gccactcatt cattagacta
     2101 gcgatttctt taggtgcttg agtataaagc tcatggccaa agttttctaa aaaaatagta
     2161 tcaaaatagt ctggcaattc ttttagggct tcctctctcc atgtagcttc attaggatag
     2221 cgaggactaa taaacaaggt atctcccact tctctcttaa aagcttgtat ttttctccgt
     2281 aggcggagta tcgcttctat attttcataa tttatagcca actcatatct attatactca
     2341 acattccagt gataggactg tcttacagct ttctccatat tttctgacca atgctttgct
     2401 tcagattttt ctttagaagt aagaagagct aagtccgaaa caatttgaga tttgatataa
     2461 tttttagttt cctctaactc tgtatccaaa ggtaaaatct tatctaaatc tagatagcca
     2521 ccatccaaaa gaatcagttt ctttacttct tcaaattccg atgcggaata acgagctaaa
     2581 tctcctccaa gagaatgacc tatcagacag atagattctt cctctacaat ctcattttta
     2641 aaccatgatt tcaattctgt ttcatctcga agatgctttt catatggatt tagaaaatag
     2701 acctgcgaat ctagttcttg aaaaaaatcc ttgctatgat aggcattgct tcccaaaccg
     2761 ctaataaaat att
//
LOCUS       DAVMEF010000179         2770 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVMEF010000179 DAVMEF010000000
VERSION     DAVMEF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.179"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            28..243
                     /locus_tag="VYQ30_001839"
     CDS             28..243
                     /locus_tag="VYQ30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966897.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(443..1207)
                     /locus_tag="VYQ30_001840"
     CDS             complement(443..1207)
                     /locus_tag="VYQ30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1966898.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(1207..2730)
                     /locus_tag="VYQ30_001841"
     CDS             complement(1207..2730)
                     /locus_tag="VYQ30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1966899.1"
                     /translation="MLGLVNVAQADEYLRIGMEAAYAPFNWTQDDDSNGAVKIDGTNQ
                     YANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMIIAGMSPTAERKQEIA
                     FSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYDLIAQIPGAKKETAM
                     GDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGFKTGEEDTAIAIGLR
                     KNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSSSFFSQVAKILSENW
                     QQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYGLQKLVGWVLNVYIE
                     IFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYMTEIVRGGILAVDKG
                     QFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTSVLNVISVVELYFSG
                     NTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTYTTGANQMQTEDLK"
BASE COUNT          755 a          543 c          625 g          847 t
ORIGIN      
        1 ttgtaaagaa gaaaatagga gaaaaacatg gctaagtcaa actttgaaaa agtagaatca
       61 gttgttggct gggttcgtga taagaaaatc acaggctacc gtatctctaa agaaacgaat
      121 gcgcgtgaaa tgtctatcat tgctctggcg cagggtcgtg caaaagtaaa aaatatttca
      181 tttgaaacag ccctaggcct aattgatttc tatgaaaaaa attatgaaaa atttgaagat
      241 taatctttgg ataacagcgg attcttgacc ttcaagtagt agagatagag aatctgcctt
      301 ttcattttga ggacagcaaa aagactgcac ggttgatgca gccttttctt tttatttgat
      361 actcttcgaa aatctcttca aaccacgtca gcttccatct gcaacctcaa aacagtgttt
      421 tgagcaacct gcagctagct tcctagtttg ctctttgatt ttcattgagt atgagataac
      481 gttgaaggaa ttcttttgta cggtcttctt taggattagt gaagaggtct tctggtttac
      541 cttcttcagc aatcacaccc ttatccataa agataacacg gtgagagaca tcacgggcaa
      601 attccatttc atgggttacg acaatcatgg tcaagccttc ctgagccagg tcctgcatga
      661 ttttgaggac ttctccaacc atttctggat cgagagctga tgttggttca tcaaagagaa
      721 tagcgtccgg attcatggag agggcacgag cgatggccac acgttgtttt tgaccacctg
      781 agagttgttt tggtttggct tgccagtagc gttctcccat gccgaccttt tccaggtttt
      841 ctttggcaat cttttcagct tctgtgcgtt cgcgttttag gacagttgtc tgagcgacga
      901 ttgtgttttc aagaacgttg agattttcaa agaggttaaa ggattggaaa accatcccta
      961 acttttcacg gtattgcgtg aggtcatagc ctttttcgag gacgttttgt ccatgataaa
     1021 ggatttgtcc atcagttggc gtttcaagta ggttaatgga gcgtaggaag gttgatttcc
     1081 cacttccaga gcttccgatg atagaaataa cttctccctt gtggacagtg agagaaatgt
     1141 cttttagcac ttcgttttgt ccataggatt ttttgaggtg tttaatttca aggattgctt
     1201 gtgtcattat ttcaaatcct ccgtttgcat ttggttagca cctgtagtgt aggtatccat
     1261 gtccattctg cgctcgataa agcgtaggat acgtgttacg gtgaaggtga ggacaaagta
     1321 aatcacggcg atgattgtaa atgtctggaa gtattgatag gtttgtgttg ccacggtatt
     1381 tcctgagaaa taaagttcga caacagagat aacgttcaat acagatgtat ctttgatatt
     1441 gatgacaaat tcattaccag ttgcaggtag gatgttacgg actacctgag gtaggacaat
     1501 cttacgcatg gtctggttat gggtcatacc aagagcagtc gcagcttcaa attgtccctt
     1561 gtcaactgct aggataccac cacggacgat ttcagtcatg taggcaccgg tattgattga
     1621 aacgatgaag atagcagcca gtgtacggtc aaggttgatc ccgaaagctt gggcagttcc
     1681 atagtagata accatcgatt gaacaatcat tggcgtacca cggaaaattt caatgtagac
     1741 attgagaacc cagccgacta gtttttgaag gccataaatg gctttgtttt cagagagagg
     1801 agcagtacgg aagacaccaa tggcaagtcc aataatgaga cctatgatgg ttccgacgat
     1861 agagattaaa agagtgatac cagcaccacg caagagttgt tgccagtttt cagaaagaat
     1921 tttagcgact tggctaaaga aactactgct agtctcttca gttgttgtag cttcggcagg
     1981 ttgttccttg atcatacgat ccatcaaggc aacttggtca tcttttgaaa tggtttcaat
     2041 gctggcattg atttggctaa tacgattgtc atttttacga agcccgatag cgatagctgt
     2101 atcttcttcc ccagttttga aaccaggttc tacttgaatc atcttgaact tagagttcgc
     2161 agcttcagca gtcagtgctt ctggacgttc agaaacataa gcatcaatga caccagcctc
     2221 aagagcttgt cgcatttgag cgaagtctcc catggctgtt tcttttttag cacctgggat
     2281 ttgtgcaatc aagtcgtaga ggtagacccc ttgttgagaa gtgatttttg caccgttaaa
     2341 gtcatccaaa gatttagcac ttgcgtaggc agaatctttt ttgacaagca aaactggttc
     2401 gctagtgtag taactgctcg aaaaggcaat ttcttgtttg cgttctgcag ttggactcat
     2461 acctgcgata atcatgtcaa tcttaccaga agtaagggca gggactagac cttcccactt
     2521 ggttttaaca accaaaggtt ctttacctaa gtccttagcg attttcttgg cgatttgaac
     2581 atcgtatccg ttggcatact gattggtccc atcgattttg acagctccgt tgctatcatc
     2641 atcctgggtc cagttaaagg gagcatatgc tgcttccatc ccgatgcgta aatattcatc
     2701 ggcttgagca acattgacaa gtcctagcat cagcaagaga cttgtgaaaa tagataagta
     2761 tatttttctc
//
LOCUS       DAVMEF010000180         2730 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVMEF010000180 DAVMEF010000000
VERSION     DAVMEF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.180"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(56..286)
                     /locus_tag="VYQ30_001842"
     CDS             complement(56..286)
                     /locus_tag="VYQ30_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1966900.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VYQ30_001843"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VYQ30_001843"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1966901.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VYQ30_001844"
     CDS             complement(1113..2036)
                     /locus_tag="VYQ30_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1966902.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VYQ30_001845"
     CDS             complement(2033..2722)
                     /locus_tag="VYQ30_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1966903.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT          898 a          598 c          400 g          834 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaattgatt tagcatagtt aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcta ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caataccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgcagccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggctg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact
//
LOCUS       DAVMEF010000181         2617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVMEF010000181 DAVMEF010000000
VERSION     DAVMEF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.181"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..330
                     /locus_tag="VYQ30_001846"
     CDS             <1..330
                     /locus_tag="VYQ30_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1966904.1"
                     /translation="SASASTSASTSASTSASASASTSASESASTSASASASTSASESA
                     STSASASASESASTSASASTSAGKSRQQLPNTGTEASKSSVLLGALAAVTGLGLFAKR
                     RKRDDEE"
     gene            377..1777
                     /locus_tag="VYQ30_001847"
     CDS             377..1777
                     /locus_tag="VYQ30_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1966905.1"
                     /translation="MHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYGYVDQVLTTIKSICYHNRSIR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEMINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHNKVEQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEIINQFARLGKTILSFENTKTYEAGQEVYA
                     VDQVQAMIEKLREISK"
     gene            1774..>2617
                     /locus_tag="VYQ30_001848"
     CDS             1774..>2617
                     /locus_tag="VYQ30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV1966906.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVY"
BASE COUNT          832 a          457 c          553 g          775 t
ORIGIN      
        1 tcagcttcag catcaaccag cgcaagtacg tcagcatcaa cgagcgcctc agcttcagca
       61 agcacctcag cgtctgaatc ggcatcaacg agtgcctcag cttcagcaag cacctcagcg
      121 tctgaatcgg catcaacgag tgcctcagct tcagcgtctg aatcggcatc aacaagcgca
      181 agcgcgtcaa cttcagcagg taagtcaaga cagcaattgc caaatacagg tacagaagct
      241 tccaagtcat ctgtactcct tggagctttg gcagctgtga ctggcttggg tttgtttgcg
      301 aaacgccgta aacgtgatga tgaagaataa tcggattaga aaaggtagtt tatcaagagt
      361 ttggacagaa aagtggatgc acgctttagt tgatgctatg tctgaacgta ttacgctact
      421 agctaatatg ggttatcctc tagagaaaca cttggcagtt tatcgtcaga tgttggaagt
      481 cagtctcgcc aacggtcaag ctagtggttt atctgataca gcaacgtata aagagtttga
      541 aatgaaaaaa agacttttaa atcagttaac ggtacaagaa tcaagtgaaa agaaagccat
      601 tgtcctagca gcaaactatg gctatgtaga ccaagtttta acgacaatca agtctatttg
      661 ttatcataat cgttcgattc gtttttatct gattcatagc gattttccaa atgaatggat
      721 taagcaatta aataagcgct tagagaagtt tgactcagaa atgatcaatt gtcgggtaac
      781 ttctgagcaa atttcatgtt ataaatcgga tattagttac acagtctttt tacgctattt
      841 catagctgat ttcgtgcaag aagacaaggc cctctacttg gactgtgatc tagttgtaac
      901 gaaaaatctg gatgacttgt ttgctacaga cttacaagat tatcctttgg ctgctgttag
      961 agatttcggg ggaagagcct atttcggtca agaaatcttt aatgccggtg ttctcttggt
     1021 aaacaatgct ttttggaaaa aagagaatat gacccaaaaa ttaattgatt taaccaatga
     1081 atggcataat aaggtggaac aggctgatca aagtattttg aatatgcttt ttgaacataa
     1141 atggttggaa ttggactttg attataatca tattgtcatt cataaacagt tcgctgatta
     1201 tcaattgcct gagggtcagg attatcctgc tattattcac tacctttccc atcggaaacc
     1261 gtggaaagat ttggcggccc aaacctatcg tgaagtttgg tggtactatc atgggcttga
     1321 atggacagaa ttgggacaaa accatcattt acatccgtta caaagatctc acatctatcc
     1381 aataaaggaa cctttcactt gtctaatcta tactgcctca gaccatattg aacaaattga
     1441 gacattggtt caatccttgc ctgatattca gtttaagata gcagctagag taatagttag
     1501 tgatcgattg gctcagatga caatttatcc aaacgtgact atatttaacg gaattcacta
     1561 tttggtagat gtcgataatg aattggtaga aaccagtcaa gtacttttag atattaatca
     1621 tggtgaaaaa acagaggaaa ttatcaatca atttgcaaga cttggaaaaa ctattttgtc
     1681 ctttgaaaat accaaaacct atgaagcagg tcaggaggta tatgctgttg accaagttca
     1741 agcaatgatt gagaaattga gagaaataag caaatgaaga aaaatcattt agtaggagat
     1801 gctctgattt tgacggttag tgatcagatt gaagagttgg attatctcct tgaatccttg
     1861 tcaaatatct gttttcatat tgcagcacct gttcaatttt ccgagaaaat ccgtagctta
     1921 gaaacgaact ataatgtccg tctccgaacg attacaaatg aagaacagct aaacttttta
     1981 gtcgatacct gtgacttttt actagatatc aatcactttc aagaagtaga tgctattgtc
     2041 tccaagtttg tacaggctgg aaaatctgtc tttgcatttg acaatacagt ccatggaaat
     2101 caggggcagg aagtttttct atcaagtaca ccagacaaat tggtttctag agtgagagac
     2161 tatctgaatg aagtcagagt aggcaccaat catcaagaga aaatcattca agatgggact
     2221 tggaatgtct ttaaaatcga tgataaggct cacttcattg taggagccaa tgtcgcctgt
     2281 agaaattttg aaaattttca tgtctcttct ggtaagttaa tcctgaatga tggtgttttt
     2341 atcaacaact cttgtagttt taactgtatg gaaagaattg aaatcggagc tggtaccatg
     2401 atgggagagg gtgttcgttt ttatgatcac gaccatattt ataccgctga aaagattgaa
     2461 aagtggcagt ggactacagc gccgattaga gttggcagag actgctggat aggaagtaat
     2521 gtcacgattt taaaaggagt gacgattgga gataatacca ttattggagc aggttgtctc
     2581 atacgcaatg atattccatc aaacagtgta gtttata
//
LOCUS       DAVMEF010000182         2606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVMEF010000182 DAVMEF010000000
VERSION     DAVMEF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.182"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(285..842)
                     /gene="sdbB"
                     /locus_tag="VYQ30_001849"
     CDS             complement(285..842)
                     /gene="sdbB"
                     /locus_tag="VYQ30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1966907.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(853..1560)
                     /gene="ccdA2"
                     /locus_tag="VYQ30_001850"
     CDS             complement(853..1560)
                     /gene="ccdA2"
                     /locus_tag="VYQ30_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1966908.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1791..2195)
                     /locus_tag="VYQ30_001851"
     CDS             complement(1791..2195)
                     /locus_tag="VYQ30_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966909.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(2237..2437)
                     /locus_tag="VYQ30_001852"
     CDS             complement(2237..2437)
                     /locus_tag="VYQ30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966910.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
BASE COUNT          763 a          555 c          469 g          819 t
ORIGIN      
        1 gactaccgta acttctcaaa gtaacttcca tctctctccc aaaactggaa gttactctaa
       61 gacgactctg ttttagggct atttgtcgtg cactttttct gagtcttttc gcttttgaac
      121 ccttatatat caagaaaaag aaaaccagtg gaagttagtc taagacggat ttccactgat
      181 gtcacgcgtt tttctcatac taaaagctag gttgtgggaa actggaagtt agttttagaa
      241 gttaccaaaa tctgactaca tcttgtaatc agattttatc gattctagtt catttcttta
      301 aatgctgctt ctgcatccgc attactgata gcaccaaatt gaatctttcc aatctttcct
      361 tggctatcaa ttaaatattc tgtaggaatg cttcgaattt gataagcttg gaaggtggtt
      421 gctttggtat cataaagaac tgggatatcc ttatatcctt gttcctggaa ccattgtggg
      481 aattgctcaa cagttttttc accttgaatt cctggtgcaa tgacagtaag aatttcgaaa
      541 tcacgatctg gtttcgccgc tagttccatc aactctggca tacttttctt gcatggacca
      601 caccatgaag cccaaaactt caagtaaacc tttttaccct taaaatcaga taacttaact
      661 tctttgccat ccatggattg caatgtgaag tctggagcat cttttccaac agcaatttgt
      721 tgtacagtcg tttgttgttt tggctgttgt gctgcttgag tctttttagt ttcttcctca
      781 ccacaggcca tcaatacaac taatgacaag agacttaagc cagcaaacat tacttttttc
      841 atttgtcctc ctttattcaa aaattccagc tagaacattt acttgtccta atagtaacaa
      901 aattcccatt aaaacaatga ggaaaccacc aattttcttt agtagcatca tatgacgctt
      961 gattttacta aaatatggca tgactagacc tgaagctagt gccaatacca agaaaggaag
     1021 ggccatgccc agagtgtaaa tgagagtata aatcgctcct tgccaagcac cattgcctcc
     1081 agaagccgca agtgctaaaa cagaacttaa aactggacca atacaaggtg tccaaccaaa
     1141 gctaaaggta ataccaagta aaaaagctga ccaataacga ttagaatctg attttttaaa
     1201 ggtaaaactt ttttgaactt ctaatttctt caaatgaaaa atttccatct ggtgaagacc
     1261 caaaatgata ataatagctc ccatgccata tcgaaaccaa tttgcataga gaatatgacc
     1321 aaagtaacca gcaccaaagc ctagaataaa gaaaatgaga gagataccag cgataaagca
     1381 aagtgttcga atcaagcctg accagagaac ctttctccca aacaaagaaa agctttttgc
     1441 actttcttga tcatccaata aaatcccagc atagactggc agaagaggaa aaatacaagg
     1501 agaaaaaaag gataaaacac ctgctagaaa aacagagatt aaaaatacta tcgtttccaa
     1561 taaagaacca actttcttaa taattctaat cctattttac tatattcaat tttatttgta
     1621 agctttctgc tacgcaaaat cgtatcgggc actattggac caatcttttc ttttgctagt
     1681 caaggcggat cttatccccc aaaatagcca aaaagcaacg acaaggatta ctcatcgctg
     1741 cttttgtgaa cgaaaatgtc ttttaggtct gacatttcat aaatcatgtt ttacttgagt
     1801 ttgtcaagga ttgctttaag ctcctctact agtttagttt ctgtctctgc tgagccattt
     1861 tcttctttca cgaaatcaag ggtttcttgg agaaggtttt gggctttggc aaggactttt
     1921 ttatccgctt tttctgcatc tagctgtcct agaaccttga tcaattccgt gcttaattgc
     1981 tggatttctg actctttctt acggcgaatc agccagaagg caatcacgcc taggagggca
     2041 agtagactga ccacaatcac tcctgccgga actgagtttg tttcagtcat cttatctgaa
     2101 tccttactat cttccgttcc ttgttttgca tccttcttgt cctgtgcagg cttgctgtcg
     2161 ctagcatttg ctttcacatc tttgagagag tccaaggcag cccagccttc acagactcta
     2221 ctgcagtatg cagaccttac tctgtcaagg cactatcttc cggagctttt tgagcatcta
     2281 ggaggacagc cttggttgca tcgattttcg gatcagatac tgttgccaaa gctttcaagc
     2341 gttggtctaa ctcttgactc aaggcacgaa gttcagactt gtcaacttgc tcttgagctt
     2401 gtgtgctcgt tgagctagcc gaagcgcttg ctaccactct aggatcttga gtcggagctg
     2461 agcttggagc tgggacaggg cttgcaggtt gactaggaac agttatggta tattgaaact
     2521 agaatagtac atatggactt ctaaaacatt gttagaattc gattttactg tcctgatcga
     2581 tttatcctat tcttatttca ttttac
//
LOCUS       DAVMEF010000183         2464 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVMEF010000183 DAVMEF010000000
VERSION     DAVMEF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.183"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..699
                     /locus_tag="VYQ30_001853"
     CDS             <1..699
                     /locus_tag="VYQ30_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV1966911.1"
                     /translation="FFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQASSGKF
                     SISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLHQMEI
                     LHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAASGGS
                     GAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIVMGFL
                     VLFGNASILSQLFE"
     gene            719..1285
                     /locus_tag="VYQ30_001854"
     CDS             719..1285
                     /locus_tag="VYQ30_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1966912.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            1296..2408
                     /gene="msrB"
                     /locus_tag="VYQ30_001855"
     CDS             1296..2408
                     /gene="msrB"
                     /locus_tag="VYQ30_001855"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1966913.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT          705 a          463 c          577 g          719 t
ORIGIN      
        1 ttcttttttc taagtgtctt tttggcaggg attctatcct tcttttctcc ttgtatctta
       61 cctttgttac cggtctatac aggagtgtta ctagatgata aggatggtgc tcaggcttct
      121 agcggcaaat tttcaatctc agttactagt ttattacgaa ctctggcctt tatagcagga
      181 atttccttta tatttatttt gttgggctat ggagctggtt ttttaggcga tttgctttat
      241 gcttcttggt tccaatatct tactggggca attattatcc ttcttggttt gcaccaaatg
      301 gagattctac actttaaggg gctttataag gaaaagaggc tacaactgca aggacagggg
      361 caaaatggta agggctatag tcaggcattt ttattgggct tgacctttag ttttgcttgg
      421 acgccttgcg tggggccggt tctggggtct gttttggcct tggcggcttc aggtggttca
      481 ggagcttggc agggagctgg tctcatgttg gtgtatacgc tgggcttggc gctaccattc
      541 ttgcttctag ctctgacctc tagttatgtt ttgaaacatt tccgaaaact tcatccctat
      601 ctcggaatcc tcaaaaaagt gggtggtttt ctcattattg tgatgggatt cttggttctg
      661 tttggaaatg cttcaatttt aagtcaatta tttgaataaa atggaaagga atatcaatat
      721 gaaaaaatgg caaacatgtg ttcttggagc aggttcgctc ctttgtttga cggcttgttc
      781 aggcaagtcc gtgactagtg aacaccaaac gaaagatgaa atgaagacgg agcagacagc
      841 tagtaaaaca agcgcagcta aagggaaaga ggtggctgat tttgaattga tgggagtaga
      901 tggcaagacc taccgtttat ctgattacaa gggcaagaaa gtctatctca aattctgggc
      961 ttcttggtgt tctatctgtc tggctagtct tccagatacg gatgaaattg ctaaagaagc
     1021 tggtgatgac tatgtggtct tgacagtagt gtcaccagga cataagggag agcaatctga
     1081 agcggacttt aagaattggt ataagggatt ggattataaa aatctcccag ttctagttga
     1141 cccatcaggc aaacttttgg aaacttatgg tgtccgttct tacccaaccc aagcctttat
     1201 agacaaagaa ggcaagctgg tcaaaacaca tccaggattc atggaaaaag atgcaatttt
     1261 gcaaactttg aaggaattag cctaggaggc gtcttatgaa tgataagtta aaaatcttct
     1321 tgttgctagg agtatttttt ctagccataa ccggtttcta tgttctattg atacgaaatg
     1381 cagggcagac agatgcctcg caaattgaaa aggcggcagt tagccaagga ggaaaagcag
     1441 tgaaaaaaac agaaattagt aaagacgcag acttgcacga aatttatcta gctggaggtt
     1501 gtttctgggg agtggaggaa tatttctcac gcgttcccgg ggtgacggat gccgtttcag
     1561 gctatgcaaa tggtagagga gaaacaacca agtacgaatt gattaaccaa acaggtcatg
     1621 cagaaaccgt ccatgtcacc tatgatgcca agcaaatttc tctcaaggaa atcctgcttc
     1681 actatttccg cattatcaat ccaaccagca aaaataaaca aggaaatgat gtggggaccc
     1741 agtaccgtac tggtgtttat tacacagatg acaaggattt ggaagtgatt aaccaagtct
     1801 ttgatgaggt ggctaagaaa tacgatcaac ctctagcagt tgaaaaggaa aacttgaaga
     1861 attttgtggt ggctgaggat taccatcaag actatctcaa gaaaaatcca aatggctact
     1921 gccatatcaa tgttaatcag gcggcctatc ctgtcattga tgccagcaaa tatccaaaac
     1981 caagtgatga ggaattgaaa aagaccctgt cacctgagga gtatgcagtt acccaggaaa
     2041 atcaaacaga acgagctttc tcaaaccgtt actgggataa atttgaatcc ggtatctatg
     2101 tggatatagc aactggagaa cctctctttt catcaaagga caagtttgag tctggttgtg
     2161 gctggcctag ttttacccaa cccatcagtc cagatgttgt cacctacaag gaagataagt
     2221 cctacaatat gacgcgtatg gaagtgcgga gccgagtagg agattctcac cttggccatg
     2281 tctttacgga tggtccacag gacaagggcg gcttacgtta ctgtatcaat agcctctcta
     2341 tccgctttat tcccaaagac caaatggaag aaaaaggcta cgcttattta ctagattatg
     2401 ttgattaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtag
     2461 ggtt
//
LOCUS       DAVMEF010000184         2433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVMEF010000184 DAVMEF010000000
VERSION     DAVMEF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.184"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            107..1114
                     /locus_tag="VYQ30_001856"
     CDS             107..1114
                     /locus_tag="VYQ30_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1966914.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1117..1317
                     /locus_tag="VYQ30_001857"
     CDS             1117..1317
                     /locus_tag="VYQ30_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1966915.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1469..1651
                     /gene="rpmF"
                     /locus_tag="VYQ30_001858"
     CDS             1469..1651
                     /gene="rpmF"
                     /locus_tag="VYQ30_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1966916.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1667..1816
                     /gene="rpmG"
                     /locus_tag="VYQ30_001859"
     CDS             1667..1816
                     /gene="rpmG"
                     /locus_tag="VYQ30_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1966917.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2296..>2433)
                     /locus_tag="VYQ30_001860"
     CDS             complement(2296..>2433)
                     /locus_tag="VYQ30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966918.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT          805 a          465 c          449 g          714 t
ORIGIN      
        1 aagaaattat tgggctgaaa ctttttagtg attattttca actttctatt gacttttaaa
       61 aacaaagtga tatcatttag atatcaaata atgggaggtc attcttatga ctgaaaaact
      121 aatcaattca aaaccaaatg gtgtattcgc attgattctc attgagttga caatcgtact
      181 tggtatcttt atatttataa tgggggttgg ttcggaaaac atttttggaa ttattatcgg
      241 acctttacta atcgtaattg caggtctagc tcatgctggt ttaaaagttg tcaaacctca
      301 ggaagctctg gttctgacac tctttggtaa ttatacaggt accatcaaag aacctggttt
      361 ttactttgtc aatcccttca gcgtagcagt caaccctgca aaccacactc gacttggaca
      421 aagtggtgat gttagcacaa aatctccttt tttaggagct aaatcatcaa atgacaatga
      481 tgtaaatctt gaaattggca agaaacacat ttccctcaaa gtcatgacct tgagcaattc
      541 tcgtcaaaaa atcaatgatt gcttaggaaa ccctgtagaa atcggtatcg cggtaacttg
      601 gagagttgtc gataccgcta aggcagtctt caacgttgat aactacaaag aatatctttc
      661 attgcaatgt gatagcgccc tccgtaatat tgtccgcatc tatccttacg atgtgtctcc
      721 taatgtggat actacgggtg acgggcaagc agatgaaggt agtctccgtg gctctagcga
      781 aattgttgct aaccgtattc gtgaagaaat ccaaagtcgt gttgaggatg ctggcttgga
      841 aatccttgaa gcacgtatca cttacctagc ttatgctcca gaaattgctg ccgttatgct
      901 tcaacgccaa caagcatctg ccattattga tgcacggaag atgattgtag atggtgctgt
      961 aggaatggtt gaaatggcac tagaacggct caatgaaggg gaattggtag aacttgacga
     1021 agaacgaaaa gctgccatgg tttcaaatct ccttgtcgtt ctttgtggca atcatgatgc
     1081 acaaccaatt gtcaacacag gaagtcttta ctaaagatgg ctgaagctaa aaaaaagcag
     1141 atcccccttc gactctcaac aaagttatac gctgcactcg catcatgggc agaagacgac
     1201 tttcgttcag tcaatgggca aatcgaatat ctcctcacag agtgtgtcaa gcaacggaag
     1261 aaagacggaa aatacgtatc agaaaccatt gacgaaccat ttgagattga tatttaacac
     1321 cttctcctgt ctgccgtgtc ggataggaga attttttcat ctttttttgt caagtaactt
     1381 tactttacaa aaaaaatgtg ttatcctagt atggttgatg aaaatcagta gattgaatcg
     1441 aatataaata cctaaaggag aaatcaaaat ggcagtacct gcacgtcgca cttcaaaagc
     1501 gaagaaaaac aaacgtcgta cacactacaa agtaacagct ccatctgtaa actttgacga
     1561 aactactgga gattactcac gttctcaccg tgtatcactt aaaggatact acaaaggacg
     1621 taaaatcgct aaagctgcat cagctgaata atagaaggga gataccatgc gcgtaaatat
     1681 tacacttgaa cacaaagaat ctggtgaacg cttgtacctt acttctaaaa acaaacgtaa
     1741 cactccagac cgccttcaat tgaagaaata ctcaccaaaa cttcgcaaac acgttgtgtt
     1801 tacagaagtg aagtaagcat tcaatacgaa tcaatacaga agaaaaacgc ttgatcagcg
     1861 ttttttcttt ttgttaattt cactatattg caagagaaat cattcgaatg actacatttt
     1921 ttgaaataag aatacggatt cggacgatta aaagtaagag aaactcaaat gtaagttggg
     1981 gttccttttt tgtatatttt tcaaataacg cctctacact atttgtagca aattcaccaa
     2041 ctacagttgc atcttagtta aaataagtta gaatatgtaa gtgagtacca gatataccaa
     2101 gacatcgtca ccatctaagg tatattcaaa atacaaaagt tgaccaacta gatttctgaa
     2161 tatccttata tatccattct taaaattggt ttaaatagcg tagtctttta aactagtttt
     2221 gagaatccaa aaaatcttcc tacatatgta agaagatttt ttagttcaga atgattagat
     2281 ttagctaatg gatacctatc ctacccactc accgttagca ttgactcgat agccatcggg
     2341 tgtagtcgtg tttactaata aatctcctga attataggta tagtaccatt taccagaaac
     2401 tgtaaaccaa ccagtagcca ttgaacctga ttc
//
LOCUS       DAVMEF010000185         2423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVMEF010000185 DAVMEF010000000
VERSION     DAVMEF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.185"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            9..1139
                     /gene="ugpC"
                     /locus_tag="VYQ30_001861"
     CDS             9..1139
                     /gene="ugpC"
                     /locus_tag="VYQ30_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1966919.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(1286..1432)
                     /locus_tag="VYQ30_001862"
     CDS             complement(1286..1432)
                     /locus_tag="VYQ30_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966920.1"
                     /translation="MEIKEQTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            1640..2398
                     /locus_tag="VYQ30_001863"
     CDS             1640..2398
                     /locus_tag="VYQ30_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1966921.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
BASE COUNT          788 a          404 c          513 g          718 t
ORIGIN      
        1 ttcacctcat ggtagaattg aatcttaaaa atatttacaa aaaatatcca aacagcgaac
       61 actattcagt tgaagatttc aacttgaaca tcaaagataa agaatttatt gttttcgtag
      121 gaccttcagg atgtggtaaa tcaactacac tccgtatgat tgctggtctt gaagacatca
      181 cagaaggtac tgcatctatc gatggcgtgg ttgtcaacga cgtagctcca aaagaccgtg
      241 atatcgccat ggtattccaa aactacgctc tttacccaca catgactgtt tatgacaaca
      301 tggctttcgg tttgaaattg cgtaaatata gcaaagaaga cattaacaaa cgtgttcaag
      361 aagcagctga aatacttgga ttgaaagaat tcttggaacg taaaccagct gacctttcag
      421 gtggtcaacg tcaacgtgtt gccatggggc gtgcgattgt ccgtgatgcg aaagtattct
      481 tgatggacga acctttgtca aacttggatg ccaaacttcg tgtatcaatg cgtgctgaaa
      541 tcgctaaaat tcaccgtcgt atcggagcta caactatcta tgtaactcac gaccaaacag
      601 aagcgatgac acttgcagac cgtatcgtta ttatgtcagc tactaagaac cctgctggta
      661 caggtactat cggacgtgta gaacaaatcg gtactcctca agaagtttac aaaaatccag
      721 ttaacaaatt cgttgcagga ttcatcggaa gcccagctat gaacttcatc aacgtgaaat
      781 tggttggtag cgaaattgtt tctgacggtt tccgtttgaa agtgccagaa ggagcattga
      841 aagttcttcg tgaaaaaggc tacgaaggaa aagaattgat ctttggtatc cgtccagaag
      901 acgtgaatgc agaacctgct ttccttgaaa cattcccaga ctgtgttgta aaagcgacta
      961 tctctgtatc agaactgctt ggttcagaat ctcaccttta ctgccaagtt ggtaaagacg
     1021 agtttgttgc aaaagttgat gctcgtgact acttgcaaac aggtgcaaca gttgagcttg
     1081 gatttgactt gaacaaagca cacttcttcg atgtagaaac tgaaaaaaca atctactaaa
     1141 ataaataaaa ttcaaagcac tacaagaaaa gatatctctt tatcaattgt agtggagaga
     1201 tatcagttaa tctagggaga gaaacaaaat gcttctctcc tttttgctag ggaagtcata
     1261 ttatgcatct atattgtgat gctctttaat actcttcgaa aatctcttca aaccacgtca
     1321 acgtcgcctt gccgtacgta tgattactga cttcgtcagt tttatctgca acctcaaagc
     1381 tgtactttga gcagcttacg gctagtttcc tagtttgctc tttgatttcc attgagtatt
     1441 atttgtgggt accatctaca agtgaagcta tatgcgtaaa ctacgtgagc aattgaattc
     1501 gaactagaga ggtaataata aatttatgct atagttatgg tgacttgtat gcttttgatt
     1561 ctagtttatc aaataataga ttagaattgt cagataatat cattttgtgt tataatgaag
     1621 aaaaaacaga ggtgttcaaa tgtcagaagc aggtcataag tttttagcaa aattggggaa
     1681 aaaacgctta cgtccaggtg gaaagcgtgc cacagattgg ttaattgcag aaggaggatt
     1741 ttcaaaagaa aagagaatac tagaggttgc gtgtaatagg ggaactacag caattgagtt
     1801 ggcacagcgt tttggttgca agataactgc tgttgatatg gatgctcaag ctttagaagt
     1861 ggctaaaaaa tctgctggaa cggcaggtgt tgctcattta atcagttttg aaagagcaaa
     1921 tgcaatgaaa cttccttatc aagatgctag ttttgatatt gttataaatg aagctatgct
     1981 gactatgcaa gccgatcaag ctaagaaaaa atgtgtaatg gaatatctaa gggtattaaa
     2041 acctggaggt cttctcttga cacatgatgt gcttcttaag gaagctaaag agtctatcag
     2101 acaggaatta tcacaagcaa ttcatgtaaa tgtaggtcct ttaactcaag atggttggga
     2161 acaggtgatg atagaatcag gttattgtga tgtgaaagca ttgactggtg aaatgacatt
     2221 aatgaaatta tcgggtatga tttatgacga aggtttgcta ggaactttga aaatttgtgt
     2281 aaatgcttgt aaaaaggaga atagaaagca gtttttaact atgtataaaa tgtttgctaa
     2341 gaataaacag aaattgggct ttattgcgat ggctagttat aaatcgtcaa aacgttagat
     2401 aattattgaa gttaactttt cct
//
LOCUS       DAVMEF010000186         2419 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVMEF010000186 DAVMEF010000000
VERSION     DAVMEF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.186"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            89..2413
                     /gene="pflB"
                     /locus_tag="VYQ30_001864"
     CDS             89..2413
                     /gene="pflB"
                     /locus_tag="VYQ30_001864"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1966922.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          759 a          526 c          479 g          655 t
ORIGIN      
        1 ttttcacttg ttgaaatcgg ttactatatg gtatacttgt cttatgaatg taacagatga
       61 ctgttactag aaaaaagagg acattaatat ggttgttaag acagttgttg aagcacaaga
      121 tatttttgac aaagcttggg aaggcttcaa aggcgtagat tggaaagaaa aagcaagtgt
      181 atcacgattt gtacaagcta actacacacc ttatgatgga gacgaaagct tccttgcagg
      241 accaacagag cgttcacttc acatcaagaa aattgtagaa gaaactaaag cacactacga
      301 agaaactcgt ttcccaatgg acactcgtcc aacatctatc gctgatatcc ctgctggatt
      361 tatcgacaaa gaaaatgaag ttatcttcgg tatccaaaac gatgaactct tcaaattgaa
      421 cttcatgcca aaaggtggta tccgtatggc tgaaactact ttgaaagaaa atggatacga
      481 accagaccca gctgttcacg aaatcttcac taaatatgta acaacagtta acgacggtat
      541 tttccgtgcc tacacttcaa atattcgtcg cgctcgtcac gcacacactg taactggtct
      601 tccagatgca tactcacgcg gacgtatcat cggtgtttac gcacgtcttg ctctttacgg
      661 tgcagactac ttgatgcaag aaaaagtaaa cgactggaat gcaatcaaag aaatcgatga
      721 agaaacaatc cgtcttcgtg aagaagtaaa ccttcaatac caagcattgc aacaagttgt
      781 tcgcctgggt gacctttacg gggttgatgt tcgcaaacca gcgatgaacg tgaaagaagc
      841 aatccaatgg gttaacattg ctttcatggc tgtctgccgt gtgattaacg gtgctgctac
      901 atctctaggt cgtgtaccaa tcgtattgga catctttgca gaacgtgacc ttgctcgtgg
      961 tacatttact gaatcagaaa tccaagaatt cgttgatgat ttcgttatga aacttcgtac
     1021 agttaaattt gctcgtacaa aagcttatga ccaattgtac tcaggtgacc caacctttat
     1081 cacaacttct atggctggta tgggtaacga cggtcgtcac cgtgttacta agatggacta
     1141 ccgtttcttg aacacacttg acaacatcgg taactcacca gaaccaaact tgacagttct
     1201 ttggactgac aaattgccat acaacttccg tcgctactgt atgcacatga gccacaaaca
     1261 ctcttctatc caatacgaag gtgtaacaac aatggctaaa gacggatatg gtgaaatgag
     1321 ctgtatctca tgctgtgtgt ctccacttga tccagaaaat gaagaacaac gccacaacat
     1381 ccagtacttc ggtgctcgtg taaacgttct taaagccctt cttactggtt tgaatggtgg
     1441 ttacgacgat gttcacaaag actacaaagt atttgatatc gaaccaatcc gtgacgaagt
     1501 tcttgaattc gaatcagtta aagcgaactt tgaaaaatct cttgactggt tgactgacac
     1561 ttacgtagat gccttgaaca tcatccacta catgactgat aggtacaact acgaagctgt
     1621 tcaaatggcc ttcttgccaa ctaaacaacg tgccaacatg ggattcggta tctgtggatt
     1681 tgctaacact gttgatacat tgtcagctat caaatacgct acagttaaac caatccgtga
     1741 cgaagatggc tacatctacg attacgaaac aatcggtgac tacccacgct ggggtgaaga
     1801 tgacccacgt tcaaacgaat tggcagaatg gttgatcgaa gcttacacaa ctcgtctacg
     1861 tagccacaaa ctatacaaag acgcagaagc tacagtatca cttttgacaa tcacatctaa
     1921 cgttgcttac tctaaacaaa ctggtaactc accagttcac aaaggtgtat acctcaacga
     1981 agatggttct gtgaacttgt ctaaacttga attcttctca ccaggtgcta acccatctaa
     2041 caaagctaaa ggtggttggt tgcaaaactt gaactcactt tctagccttg actttagtta
     2101 tgcagctgac ggtatctcat tgactacaca agtatcacct cgcgctcttg gtaagactcg
     2161 tgatgaacaa gttgataact tggtaacaat ccttgatggt tacttcgaaa acggtggaca
     2221 acacgttaac ttgaacgtta tggacttgaa cgatgtttac gaaaaaatca tgtcaggcga
     2281 agacgttatc gtacgtatct ctggatactg tgtaaacact aaatacctca ctccagaaca
     2341 aaaaactgaa ttgacacaac gtgtcttcca cgaagttctt tcaatggatg acgccttgga
     2401 tgcattgagc taatcaagt
//
LOCUS       DAVMEF010000187         2408 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVMEF010000187 DAVMEF010000000
VERSION     DAVMEF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.187"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..2257)
                     /locus_tag="VYQ30_001865"
     CDS             complement(<1..2257)
                     /locus_tag="VYQ30_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEV1966923.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIAAVGAVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVNPNTGALENLSRMSYG
                     YSIYNTGTSIQTMLTLGSGLGKPSGVTNSITNKNGERVQYYNMSTMTKRGSGYTWANG
                     AQMNGFFAKQGYGLTSSWTVPINGTDTSFTFTPYAAKTDNIRINYFNGKGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASEFASTSASASASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSASE
                     SASTSASASASTSASASASTSASASASASASASASTSASASASASASASASTSASTSA
                     STSASASASTSASASASTSASASASTSASASASASASASASASTSASASASTSASASA
                     STSASTSASTSASASASTSASESASTSASESASTSASASASTSASASASTSASASAST
                     SASASASTSASASASTSASVSASTSASASASTSASESASTSASASASTSASASASTSA
                     SASASTSASASA"
     gene            complement(2255..2368)
                     /locus_tag="VYQ30_001866"
                     /pseudo
     CDS             complement(2255..2368)
                     /locus_tag="VYQ30_001866"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          579 a          534 c          589 g          706 t
ORIGIN      
        1 acgctgaagc cgatgcactg gttgacgctg aagctgatgc actggttgat gctgaagctg
       61 aggcgcttgt gcttgctgaa gctgaggcac ttgttgaggc cgattcagac gctgaggtgc
      121 ttgctgaagc cgaagcactg gtgcttgctg agactgatgc acttgtcgat gctgaggctg
      181 acgcactggt tgacgctgag gctgacgcgc tagtacttgc tgaagccgag gcgctggtgc
      241 ttgctgaggc cgacgcactc gttgacgctg aggctgaggc actcgttgat gcagattcag
      301 acgcactcgt tgatgcagat tcagacgctg aggtgcttgc tgaggctgac gcacttgttg
      361 acgctgacgt acttgcactc gttgatgctg aagctgatgc gcttgtcgat gctgaagctg
      421 aagcacttgt acttgctgaa gccgacgcac ttgcgcttgc tgaagctgaa gcacttgcac
      481 tcgttgacgc tgaagctgag gcgcttgtcg atgctgaagc cgacgcactc gttgatgctg
      541 aagctgaggc actcgttgac gctgacgtac ttgcactcgt tgatgctgaa gctgatgcac
      601 ttgcgcttgc tgaagctgac gcacttgtac ttgctgaagc cgacgcactt gcgcttgctg
      661 aagctgaggc gcttgtgctt gctgaagctg aagcacttgt acttgctgaa gccgatgcgc
      721 tggttgacgc tgattcagat gcactggttg atgctgattc agacgcactg gtacttgctg
      781 aagccgaagc actggtgctt gctgaagctg aagcacttgt acttgctgaa gctgaagcac
      841 ttgtcgacgc tgaggctgac gcacttgttg atgctgaagc cgacgctgag gtgcttgctg
      901 aagctgaagc acttgtactt gctgaagccg acgctgaggt gcttgctgaa gctgaggcac
      961 tggttgatgc gaattcagac gcacttgttg atgctgaagc cgacgcactg gtacttgctg
     1021 aagctgatgc gctttgacta gcacttactg agagtgactt agactgtgaa agtgactgac
     1081 tggtcgtgct agattcaact acctttcctt taccattgaa gtagttaatt ctaatgttat
     1141 ctgttttagc agcgtaaggg gtaaatgtaa aggatgtatc cgttccatta attggtacag
     1201 tccaagatga tgttaatcca tatccttgct tagcaaagaa accattcatt tgggcaccat
     1261 ttgcccaagt atacccacta ccccgcttcg tcattgtaga catattatag tattgaaccc
     1321 gttcaccatt tttgttagta atggagttcg ttacacctga aggtttacca agaccactgc
     1381 caagggttaa cattgtttgt attgaagtac ctgtattata gatagaatat ccataagaca
     1441 ttcttgaaag attttcaaga gcccctgtgt taggatttac ataggtgact gtataggtaa
     1501 aggtcaattt tgaaccatca tttgtcactg tcaatttata gtaaattgga acatgatata
     1561 ccgaatctac acctttacct gtataaactt ttgtctcact tttagcaatg tttagacttg
     1621 cattaattgc aggggcgcca tttacaatcg tattgccaga aaaaacagca gcttttatag
     1681 aagccagcaa ttgctcgatg gaatctactg aacgcttgcg tcgcttagca taagattgga
     1741 gattgacatt tgtaactgat gctacataat cactagctgg tttcttacga tcttcttcga
     1801 ccttcttagc agttgcttct gtagcggcag ctgtttgtga acctaccaca gtagatgatg
     1861 cagaaatact tgtcgaagcc gatgtacttg ctgattctga tgcacttgta ctcgctgagg
     1921 tcgaagcgct ggttgaggct gactcagatg cagatgtact tgccgactct gaagctgaca
     1981 aactagttga actcgctgaa ttacttgtag atatcgtacc tagaactact gtaccatttg
     2041 ttgccaaagc atccgttttc tctacagttt tttcaagtac tgctgactca tttgtaaata
     2101 ccttcgtttg agttgcaacg gttccactga tgacagcccc cacagcagct atccccttga
     2161 ggatatcaag cccagtaatt gaatgactta ctttatcttc taccgtttcg gtcatgacct
     2221 gagtagtatc aacaccacca cgcatgacct taaacaagcc aaaaagagaa gtcgaggccc
     2281 gtagccaatg cttccctgat ttgattaatt tgaaccgagt tactcggtct gtttctctat
     2341 attcaccctt ctgacgtcta aaaaatattt tgaaatttcc tttcgagtat tattttcatg
     2401 tatggata
//
LOCUS       DAVMEF010000188         2379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVMEF010000188 DAVMEF010000000
VERSION     DAVMEF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.188"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..1170
                     /locus_tag="VYQ30_001867"
     CDS             1..1170
                     /locus_tag="VYQ30_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEV1966924.1"
                     /translation="MLANNPVSAQESSSQATYSKSSGSWIKSGNRWWYKHSDGSYTTN
                     GWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTGWCLVSGSWYYLNSSGVM
                     QTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQNINRRALVLGETSTRAV
                     PIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFESSSEGDVNYLYFTCHGGR
                     DGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTIISKDNTGEGNEGASTEY
                     FDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSGGSLSLATKYWSLGSGWN
                     PLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHIEVYPDNSQFVLFKK"
     gene            1183..2364
                     /locus_tag="VYQ30_001868"
     CDS             1183..2364
                     /locus_tag="VYQ30_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1966925.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT          783 a          333 c          544 g          719 t
ORIGIN      
        1 ctgctagcaa ataatccagt ttctgctcaa gaatcatcct ctcaagcaac ttatagtaag
       61 tcaagtggaa gttggataaa atctggtaac cgttggtggt ataagcattc tgatggttct
      121 tatactacaa atggatggga aaaaataaat ggtacctggt actattttga tagtgaaggt
      181 tggatgaaga caggttggat taaagaatat gggaaatggt attacctaga tgatagtggt
      241 gctatgaaaa ctggatggtg tttggtttct ggtagctggt attatttaaa tagtagcggt
      301 gtaatgcaaa cgggactaca gactatcaat ggaaaacaat attatttagc tgccggcggt
      361 gctatgcaga caggttggca caatatcgga gatgatacct atttctttgc taatagtggg
      421 gaaaatcaaa atatcaatcg tcgtgcttta gttcttggtg aaacatcaac gagagcagta
      481 cctattgcag atgttaatgc tatggaaaag gtttttaata accaaaactt tagtgaagtt
      541 gttcgtttcc cagatagaac aaaatccgag ataattgcaa aaatgcaaga gctttttgaa
      601 tcttctagcg aaggcgatgt gaactatctt tactttactt gtcatggagg aagagatgga
      661 agaatctata ttggtagtga tggcctagca ttttctggtt gggaattggc gtccgttttg
      721 aaacaataca aaggtaagtt tgttgttatg ctggattgtt gtcatgctgg aacaattatt
      781 tctaaagata atacaggtga aggcaatgaa ggagcctcta cggaatattt tgatttagat
      841 gaatttgtat caggattttc taatatggat ggtaacgaaa aatcaggtga gatgattgac
      901 tctaaatttt tagtattatg ctcatcaaga ggtgcagaat actctagtgg tggatcatta
      961 agtttagcga ctaaatattg gtctttaggt tcaggatgga atcctctaca gaattcacaa
     1021 gcatatttag cagcagacca aaataataat cgcagaatca cattaaatga attgtataca
     1081 tactctcgtg aacaagtttt gaaacaaaat tctaaccagc atattgaagt ttatcctgac
     1141 aacagtcaat ttgtgttatt taagaaataa gggggcaata aaatgaaagt atcaaaaaaa
     1201 attacactat ttagtttgtc ttttgcaggt tttgttttat tgactttacc tcaagcagga
     1261 aaggcttttg aacttaaaga agactgggca tttaaaggtg gcattcgata cgagaatggg
     1321 aaagtcagca aaattaataa tggatatgaa gtaaatatta aagtgttaga tttacctagt
     1381 actagcgcaa tcgaatggac agttagattg aatggagaaa agcaaaatac taacttctta
     1441 gcggaggaaa gaactgtatc taaaactgaa gataagggac gtttcttgca cttttatatc
     1501 ccctatggat atcgtgggga tattgtagta gaggctaaga gtggaaacga agtgaagact
     1561 tggtctacta aggtagttga cgatgtttat tcagattctg ctaagagtgg ctactttatt
     1621 ctcgatgggg aacaaatctt agaaagttca tgggattccg taaatgagtc ttatattgca
     1681 acgcttccaa ctgtaacatc aggaaaaact gttgttgctt ggcgtgaaaa aggaactctt
     1741 aatttaatta aaccaggaag aattgctcgg caatataata gcagtggatc atatgtagaa
     1801 ctttctccaa tctttgaaac tgctagttgg ctaaaatcaa accaaaactg gtactatcaa
     1861 aaacaaggtc aattagttca aaattcttgg atcaaggatc agggaagctg gtactttatg
     1921 gatgatgaag gggttatgtt taatcaaaca tggctacacc aaggtggaag ctggtatgcc
     1981 tttaagtcat caggagccat gatttcagct gattggttgt atgataatgg ttcttggtat
     2041 tacctaaagg actcaggttc tatggtaaca ggatggctta aaaatggagg ctcttggtat
     2101 tacctaaaca agtcaggttc aatggctaca ggttggatta aagatagtgg aacttggtat
     2161 tatctaaaga attcaggctc tatggcgaca ggctgggtga aagatagtgg ctcttggtat
     2221 tatctaaaga actcaggttc aatggcaaca ggttgggtca aagataatgg taagtggtac
     2281 tatctcgcat catctggtaa tatgcttcgc aatacacgca caccagatgg ctattatgta
     2341 gatggcagtg gcgcttggaa ataaattttt aaaagagaa
//
LOCUS       DAVMEF010000189         2372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVMEF010000189 DAVMEF010000000
VERSION     DAVMEF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.189"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..632
                     /locus_tag="VYQ30_001869"
     CDS             24..632
                     /locus_tag="VYQ30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1966926.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            663..866
                     /locus_tag="VYQ30_001870"
     CDS             663..866
                     /locus_tag="VYQ30_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1966927.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(976..1248)
                     /locus_tag="VYQ30_001871"
                     /pseudo
     CDS             complement(976..1248)
                     /locus_tag="VYQ30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1451..1738
                     /locus_tag="VYQ30_001872"
     CDS             1451..1738
                     /locus_tag="VYQ30_001872"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1966928.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(2016..>2372)
                     /locus_tag="VYQ30_001873"
     CDS             complement(2016..>2372)
                     /locus_tag="VYQ30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1966929.1"
                     /translation="ICGLLLGLISSFLGIGGGPLNVSLLMVFFSISIKEATMYSLAII
                     FFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGGVLGTVVSKVLPENWVRYCFKGM
                     LFFVMGMTLYNLFHIL"
BASE COUNT          850 a          412 c          429 g          681 t
ORIGIN      
        1 taatttaatt aaaggagttt catatgtcaa acgaaaaaaa cacaaacact aacgtagaaa
       61 agaaagatgc tactgttgta gctcacgaaa tcaaagggga acttacttac gaagataaag
      121 ttatccaaaa aatcattggt ctttcactag aaaacgtttc aggtcttttg ggaatcgatg
      181 gtggtttctt ctcaaatctt aaagaaaaaa tcgttaacag cgatgacgta acaagtggtg
      241 ttaacgtaga agttggtaaa acacaagttg cagttgactt aaacgttatt gttgagtacc
      301 aaaaaaatgt tccagcttta tattcagaaa tcagagaaat cgtatcttca gaagttgcta
      361 aaatgactga cttggaaatt gttgaaatca acgtaaacgt tgtcgacatc aaaactaaag
      421 aacagcatga agcagactca gtaagccttc aagatcgcgt atctgacgtt gctgaatcaa
      481 caggagaatt cacttcagaa caattcgaaa aagctaaatc tggtcttgga tctggtttct
      541 caactgttca agaaaaagtt agcgaaggtg tagaagctgt taaaggtgca gcaaatggtg
      601 tagtatctca cgaaaacact cgtgtaaact aagataaaat aaatataaca ggagaaatta
      661 tcatgtcagt agaagaaaaa ttaaatcaag ctaaaggttc tattaaagaa ggtgttggga
      721 aagccatcgg tgatgaaaaa atggaaaaag aaggagcagc tgaaaaagtt gtttctaaag
      781 taaaagaagt tgccgaagac gctaaagacg ctgtagaagg tgctgtagaa ggtgttaaaa
      841 acatgttgag tggcgacgat aaataaggtt aaaagttact ttatcttttt ggtaacatta
      901 gtcaaaagag tctgagtcaa gatgattctc agaaaacaaa aagctagaga ttcccaattg
      961 cggaactcta gctttttaat tttgcctctt tctcttatta tatttcagca ggttgttggc
     1021 catgagtatg aatcccatgt caattctcac ttgacgctta cctctcagat gacatctctt
     1081 ataacccaaa caaaccttta tctgcccaaa gacagatttc atatcaatct tacgtttagc
     1141 gaaaatttgt ctacccttgg aagataaaag tgcctgatat tctttagttt ttaaacactg
     1201 gtaacgttca ttcatataca gtcccttttg aggggctgat tcaggttcat aatcgcagtc
     1261 aacattgatt tcaaggctgt ttgctttcta tctccccttt ttcatgatat attttcgtac
     1321 ggtgcttcaa tagtttctat agtaaaatga acccaaaata gtacacgttg cggtataatg
     1381 ttcttgtggc atattcatta gattttcgta aaaagttctc tcctatttgt gagcgaacag
     1441 gtatagtcaa ttgattgtca gtttgaagac caagagtcgt aaggcaaagg atatggcaga
     1501 atccatccaa gggtggctgg cacaatttct agtgaacctc tttaaatcta ttacgtttga
     1561 ctgtggaaaa gagttttcaa aatggaaaga tatttccaat catcacgata gtgagagctt
     1621 ttttgcgaat ctaggatgtc caagacagcg gtgtctcaac gaacattcaa atagactttt
     1681 aaggtgtcat gacttgccaa agcagacaga tttcaacgaa gtcagtcaag aattttaggc
     1741 tgggtaaaaa ctcaatttca ggagaaaatg aaataaatat tcccacaata aaatgcatag
     1801 tatcaaagtt tttccaacac ctgatattat gcatttttta attttaaaga ctttttccca
     1861 gcctcaagaa atcacatccc acctaaatcc tttggatacc aaatacctca tcaggtattt
     1921 tctgagttgg taagactgtc tagcttaatt tgacaaatgg agggcgttaa atccagtttt
     1981 tacaggaagt ctatttaaat attttcacac tgaccttaca aaatatgaaa taaattatat
     2041 aaagtcattc ccatgacaaa gaataacatc cctttaaaac agtatcttac ccaattttca
     2101 ggtaagactt ttgatacaac cgtccccaat acccctccgc atatagaagc aagaaaaata
     2161 actggaacag gtgctaaatg atactgattt aaacctgtaa ccacaacaat tgtagccaaa
     2221 tgagacagct gggaaaagaa aataattgca agagaataca tcgttgcttc ttttatcgaa
     2281 atagaaaaaa acaccatcaa taaagataca ttgagcggac caccaccaat ccctaaaaaa
     2341 ctagaaatta agcccaatag aagaccacaa at
//
LOCUS       DAVMEF010000190         2361 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVMEF010000190 DAVMEF010000000
VERSION     DAVMEF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.190"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            86..1840
                     /locus_tag="VYQ30_001874"
     CDS             86..1840
                     /locus_tag="VYQ30_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966930.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQEALSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1806..>2361
                     /locus_tag="VYQ30_001875"
     CDS             1806..>2361
                     /locus_tag="VYQ30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966931.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKY"
BASE COUNT          829 a          320 c          439 g          773 t
ORIGIN      
        1 gatgtataat agaaaagcaa tgatagatat tatcaattaa gcgaatttat atctaaaagg
       61 gatattaaag aaaggagata tgcttatgaa gatttacaaa aaactatttg cttatgtcca
      121 agataagaaa tatcttgggg ttttggccat aattttttct gctatatctg ctgcacttac
      181 agtatatgga tattatttaa tctacaaatt tctagataag ttaataatta attcaaactt
      241 atccggtgca gagagtatag cattaaaatc tgttattaca ctaacaagtg gagcgatatt
      301 ttattttgtc tcaggaatgt tttcacatat cttgggattc aggcttgaaa caaatttaag
      361 aaaaagggga atcgatggtc tggaaaaagc aagttttagg ttctttgact taaatccatc
      421 tggtcaaata agaaagatta tagatgacaa tgctgcacaa actcatcagg tggtagcaca
      481 catgattccc gatagttctc aggcaataat cacacccgta cttgtacttg cacttggctt
      541 tatagtaagt ataagggttg gtataacttt gcttgctctt actataattg gcggcttaat
      601 tttaggggct atgatgggcg agcaagaatt tatgaagata taccaagaag ctctatctaa
      661 actaagtgct gaaactgttg agtacgtgag aggaatgcaa gttgtaaaaa tatttaaagc
      721 aaatgtagag tcttttaaaa gcttttataa ggcgataaaa gattactcaa agtatgctta
      781 tgattattcc ctatcttgta aaaggcctta tgttttgtat caatggttat tttttggact
      841 gattgcaatt ttaattattc ctatagttta ttttatgact agcttagcta gcgcaaaggt
      901 gattttactt gagcttatca tgattttatt tttatcagga gttctctttg tttcattcat
      961 gagaatgatg tggtactcca tgtatatttc tcaaggaaat tatgcagtag atactttaga
     1021 ggcgctttac gaagatatgc aaaaagacaa attagtgcat ggtaatgtca ataattttaa
     1081 aaactataat atagaatttg agaatgttag ctttgcttat aatgataaag ctgtcattga
     1141 aaatttatcc tttaatttag aagaaggaaa gtcctacgca cttgtcggtt catctggatc
     1201 aggcaaatca acagtagcaa aacttatatc aggtttttac aatgttaata aaggaagcat
     1261 aaagataggc gggatagcaa taagtgaata ttctgacgaa gccttaatta aagccatttc
     1321 ctttgttttt caagattcaa aattatttaa gaagagcatt tatgataatg tagctttagc
     1381 taataaagat gcgacgaaag atgacgttat gagagcctta aaattagcag gatgcgattt
     1441 aatattagac aaattcccag aaagagaaaa tacaatcata ggctcaaaag gtgtttattt
     1501 atccggtgga gaaaaacaaa gaattgcaat tgctagagca attttaaagg attccaaaat
     1561 tattattatg gatgaagcat cagcatctat tgacccagat aacgagtttg aattgcaaaa
     1621 agcttttaaa aatcttatga aggataaaac agttatcatg attgcacaca ggctatctac
     1681 aattaaagac cttgatgaaa ttattgtcat ggatagtgga aaaattatag aaagagggtc
     1741 tgacaaagaa ttaatgtcaa aagatacaag gtataagagc ctgcaagaga tgtttaacag
     1801 tgcgaatgaa tggagggttt caaatgaaag agttttataa aaaaagattt gctcttacag
     1861 atggaggagc aagaaattta agtaaagcaa cactggcttc atttttcgtt tattgtataa
     1921 acatgcttcc tgccatatta cttatgattt ttgctcagga agttttggaa aatatgggca
     1981 aaagcaatgg cttttatata gtattctcag ttttgacttt gatagcgatg tatattttgc
     2041 tttctatcga atacgataaa ttatataaca caacctatca agaaagtgca gatttaagaa
     2101 taaggacagc ggagaattta tcaaaattac ctctatctta cttttctaaa catgacattt
     2161 ccgacatttc acaaacaatc atggctgata ttgaaggcat agagcatgca atgagccact
     2221 caataccaaa ggtgggcggc atggtactgt ttttcccatt aatatctgta atgatgctag
     2281 cgggcaatgt caagatgggt ttagctgtaa ttattccatc tattttaagc tttatattta
     2341 tacctttatc taaaaaatat c
//
LOCUS       DAVMEF010000191         2327 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVMEF010000191 DAVMEF010000000
VERSION     DAVMEF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.191"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..415)
                     /locus_tag="VYQ30_001876"
     CDS             complement(<1..415)
                     /locus_tag="VYQ30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1966932.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            537..1283
                     /locus_tag="VYQ30_001877"
     CDS             537..1283
                     /locus_tag="VYQ30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1966933.1"
                     /translation="MKRLEQIIKLVSEHEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1296..2276
                     /locus_tag="VYQ30_001878"
     CDS             1296..2276
                     /locus_tag="VYQ30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1966934.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          839 a          287 c          442 g          759 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccgggc
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 agtttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
      541 aaagattgga acaaattatt aaattagtat cagaacatga aaagatcgac gttaatacat
      601 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
      661 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
      721 atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
      781 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
      841 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
      901 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
      961 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc
     1021 aagcttttgt tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa
     1081 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg
     1141 actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg
     1201 aagtaataac agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga
     1261 taatggttaa ggtagtttcg taagaggtta agtgtatgaa tcaagatagg aataaactgc
     1321 tctctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
     1381 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa
     1441 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
     1501 aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa
     1561 caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg
     1621 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
     1681 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
     1741 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
     1801 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
     1861 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
     1921 gaattggtga ttttagcaat aaaggaaaac atcaatggtt agacatgctt acagaggatg
     1981 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
     2041 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc tctttggaag
     2101 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga
     2161 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
     2221 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
     2281 gatagactga ttcagtttat cgtttttctt tttagttgat tgcacat
//
LOCUS       DAVMEF010000192         2285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVMEF010000192 DAVMEF010000000
VERSION     DAVMEF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.192"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(42..1271)
                     /locus_tag="VYQ30_001879"
     CDS             complement(42..1271)
                     /locus_tag="VYQ30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1966935.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(1261..>2285)
                     /locus_tag="VYQ30_001880"
     CDS             complement(1261..>2285)
                     /locus_tag="VYQ30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1966936.1"
                     /translation="VLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVF
                     VIIALFIIWGLAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQ
                     VLTNFSSQATVLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNY
                     LTQFIPRKLKEPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLG
                     VTAGILNLVPYLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGS
                     QLNIHPINVLFVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGE
                     EVKSEQ"
BASE COUNT          758 a          517 c          398 g          612 t
ORIGIN      
        1 atcaaaagag ataaactaca gttctatatg tttaaacatt cttacaatct ctcaaacagt
       61 tcttgcattt gcacatcatc tggaaccagt tttaagtaag tgtgagcatg gacttttgct
      121 tcttcaaaat gtcccaattc acgcaagaga tagatatagt gttccagaaa ttctggattg
      181 tccttcaaat ctcctgtcaa ctcttgataa tgctcataag cagtatccaa atcgtccatt
      241 tcttgataag aacgagcaat catccacttg gtcaaaagat tttctggctc ctcactctgc
      301 aagtctagaa tatcctcata acgctcctgc tccagataaa tagtggctaa acgaagcaag
      361 atttcttctg tatcctcagc gtcttctttt gcagtaagga gataattttc tgcaccacta
      421 gcatcatgca attcataaga aaattgtgaa gcagctagca agaggcgagt ttcaaaggga
      481 tttttctcta atccttgctt agcgatacgc agggcttctt gaacttgatg ttccttatgt
      541 aaagcctgac tgtacccata ctcatagcct tcaaagtcag gagaaatggt atcaagctgc
      601 ttaaagtaga gggtggcttt ttgatattct tcttgatcaa aataaagact ggccaactca
      661 aaagctgtta agtcatcgta ttctaactcc agggcttttt ctaaaaactc agtagccgtt
      721 tcaaatttcc ctaactgagc ataggcaaag ccaattcgtt gataggtgga aatgcccgtt
      781 tgctcataaa tcgagcgatt atctaactgg gcataggctt gaatagccgc ttggtaattt
      841 tccaactcac tatccaactc tgccaaaccc aatatcaaga gagaatcctc tgagtaggtc
      901 aaggcctcca ataatttctc acgtgccaca tctgtcaaac cttccagctg gtaaaggtct
      961 gccttcagag ccaaagacga gacataccag tcactgtcag cttggatttc ctcaagatag
     1021 gtaaaggctt cttctatttg accatcctcg ctagcaattg cagctagatt aagatgaacc
     1081 tctggaaaat cctctacaat tttcaggtaa atttccttgg cctgaggata gaaaccaatc
     1141 ccttcaagat aagttgccaa ctcatacaga agatcacttg aatcattttc taaagctttg
     1201 gcgaaataat gctcagcctt agttaaatct tgctcctcca aagcctgtaa catctgttga
     1261 ctattgttca ctcttgactt cctcaccctc taattcatat agaccactga ctaccttata
     1321 ccattcgaaa atggctgaaa tgacaacctt agcagaggca taaaccggaa taccaagtaa
     1381 aactccccag ataccaaaca tagatcctga agttaacaaa acaaagagaa cattaatagg
     1441 atggatgttt aattgacttc ccaaaatcaa tggagagaca aaacggcctt caatagtttg
     1501 ttctacgata aagacaatca ctactttcaa aagcatgact ggaccagcaa tcaaacccaa
     1561 tactagagca ggaagcatgg ctagaaagct accaagataa gggaccagat ttaaaatacc
     1621 agcagtaacc cccagcgtaa ccgcatagcg tagaccaata atcttgaaga agatgataaa
     1681 cattactgct acaataatag ccactgtcac ttgccctcga acatagttgg acaactgttg
     1741 attcacatct gataaaactt gtccaacagg ttccttcaat tttcttggaa tgaattgggt
     1801 caaatagtta cgcaagcctt tcccatcacg caagagataa aagagcatga aaggaacgat
     1861 aatcaaggca acaatcactt gagaagcccc gctaataaag gcactcaccc agttgactgc
     1921 ctgagatgaa accttacttg ccaaaactgt agcctggcta gaaaaattgg tcaaaacttg
     1981 ctctaattga ggtctgaaat catctggcag gtgctgggct accaatccat taacaatcct
     2041 atctatatct tctaagtaaa caggaacgtt tcttgcaaag gtcaaaacct gacgttgcag
     2101 atttggaatg gcgactgcca agccccaaat gataaagaga gcgatgataa caaagacaat
     2161 agtgatagct ataacacgat taaccttatg cttctccatc caatcaacaa taggattcaa
     2221 caaataatat aacaaaccag acaaaatgac tggcaacatc acaactgcta taaagtctaa
     2281 aacag
//
LOCUS       DAVMEF010000193         2285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVMEF010000193 DAVMEF010000000
VERSION     DAVMEF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.193"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(131..568)
                     /locus_tag="VYQ30_001881"
     CDS             complement(131..568)
                     /locus_tag="VYQ30_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1966937.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(644..1486)
                     /locus_tag="VYQ30_001882"
     CDS             complement(644..1486)
                     /locus_tag="VYQ30_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1966938.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            1598..2248
                     /locus_tag="VYQ30_001883"
     CDS             1598..2248
                     /locus_tag="VYQ30_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1966939.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
BASE COUNT          668 a          581 c          403 g          633 t
ORIGIN      
        1 cttctattat acttccaaaa tgacactata aaggctaata caattcctat aacgagatac
       61 atttcttact cctttaatag ctacatttta tcataattat ccaaagaaaa aaagagggca
      121 tttatccctc ttaatccttc atctgactct ctgcatcggc cacgactttt tctagactgg
      181 tttgaccaag ttctgcctcc atagtcaact gaattctctc caatttttga tccaaaacat
      241 catgaatatg agctcctaca gggcaatttg gattcggatt gtcatggaaa ctgaagagtt
      301 gacctgtctt accaagacat tctaccgcct gataaacatc taaaagacta atatccttaa
      361 ggtccttgac aatctctgtt ccgcccgttc cacgcgctac tgaaatcagc tctgccttct
      421 tcaactggga caagatcttt ctgataatga caggattgac cccgacacta gcagccagaa
      481 aatcactggt caccttgctt tccttccctt cgagggcaat gattatcagc atatgagtcg
      541 caatggtaaa tctacttgga atttgcatcc tcttctcctt tttacgaggc taccctgcct
      601 ctactcttct ttttctatta ttataccctt tttagttgta atgtcaatcg ttaccacttt
      661 tcaaccagtc gtctaactcc cgatcgcagc cctctttctg agccaattct ctcaaaaatt
      721 cctgatgatg agtatggtgg atcccattga ccagactttc atagtaaacc tcaaaatagg
      781 gaagtctcag gtctttagcc agctgcaatt cagctgccac atcgtagtct acccgtcgga
      841 agtccatatc taccaggcct ttgtcatcaa actccaaaat catatactgg gcccgcaagt
      901 ccttccgtag ctgagcgtcc aaaaagaaag gttgcccaat cgaacccgga ttgacaatca
      961 attgcccacc agtcccgtaa cgaagcaact gctggtgaat atgtccataa acagcaatat
     1021 cacagggagg atgagtcacc aagcggtcaa actcctcttg tttgccagta tgaatcaact
     1081 ctcgccccca gttcttatca ggcagatgat ggctaattcc caccgtcaaa tccccaaact
     1141 gacgatgaat ttgaagaggt tgattgtgga gcacttcaat ttcttctagg gaaatttcct
     1201 ctaaaacata ctggcactgg cgcaagagat agcgttgact ggggcgagta ctatccaatt
     1261 ccttacggac accatgccaa agactgtctt cccagtttcc caaaactcta gccgtaatcg
     1321 gtagttgatc caacaagtcc aaaatccttc tacgccctgt ccctggcatg agaatatctc
     1381 ccaaaagcca gtattcatcc actcctatct gccgagcatc tgccaaaaca gcctccaagg
     1441 cggtggtatt tccatgaata tctgaaagaa gagctatttt cgtcatatcc atctcctcgt
     1501 tttttctctt gcaataagta taacataaaa agtcacagct agagaaatct agcttttttt
     1561 gatatactag ataaagatat tagacaagag gaaacgaatg accccaaaca aagaagacta
     1621 tctaaaatgt atttatgaaa ttggcataga cctgcataag attaccaaca aggaaattgc
     1681 ggctcgcatg caagtctctc cccctgccgt aactgaaatg atcaaacgaa tgaaaagtga
     1741 aaatctcatc ctaaaggaca aggaatgtgg ctatctactg actgacctcg gtctcaaact
     1801 ggtctctgag ctctatcgta agcaccgctt gattgaagtt tttctagttc atcatttaga
     1861 ctatacaagt gaccagattc acgaggaagc tgaggtcttg gaacacactg tctctgacct
     1921 gttcgtggaa agactagata aactgctagg tttccctaaa acctgccccc acgggggaac
     1981 tattcctgcc aagggagaac tactcgttga aatcaataac ctcccactag ctgatatcaa
     2041 ggaagctggc gcctaccgcc tgactcgggt gcacgatagt tttgacattc tccattatct
     2101 ggacaagcac tcacttcaca tcggtgacca gctccaagtc aagcagtttg atggcttcag
     2161 caataccttc actatcctca gtaacgacga ggatttacaa gtgaatatgg acattgcaaa
     2221 acaactctat gtcgagaaaa tcaactaatt tctcaagtcc cctaccaacc ctgaaagttt
     2281 tattt
//
LOCUS       DAVMEF010000194         2278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVMEF010000194 DAVMEF010000000
VERSION     DAVMEF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.194"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..261)
                     /locus_tag="VYQ30_001884"
     CDS             complement(<1..261)
                     /locus_tag="VYQ30_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1966940.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYE"
     gene            complement(383..>2278)
                     /gene="ftsH"
                     /locus_tag="VYQ30_001885"
     CDS             complement(383..>2278)
                     /gene="ftsH"
                     /locus_tag="VYQ30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1966941.1"
                     /translation="LVTGFQYFYSGNNSGGSQQINYTELVQEITDGNVKELTYQPNGS
                     VIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTILPADTTVSELQKLATDHKAEV
                     TVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGGGNGRNPMSFGRSKAKAANKED
                     IKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGARIPAGVLLEGPPGTGKTLLAKA
                     VAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAAPAIIFIDEIDAVGRQRGV
                     GLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNRSDVLDPALLRPGRFDRKVLVG
                     RPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFVGADLENVLNEAALVAARRNKS
                     IIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHEAGHTIVGLVLSNARVVHKVTI
                     VPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGRVAEEIIFNVQTTGASNDFEQA
                     TQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKSISEQTAYEIDEEVRSLLNEAR
                     NKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYETGKMPEAVEEESHALSYDEVK
                     SKMNDEK"
BASE COUNT          629 a          528 c          368 g          753 t
ORIGIN      
        1 ttcatagggt tctttatcgt atctacgctt ctgactttcc tgcttacgga tatagtctaa
       61 aattcgattt cgaaacttgg ttttgaaata tttccttaaa cgtggaatat cgtctaccag
      121 tccttcttct ctactaatca attcatgtaa gcagagcatg ccttcttggt cccaatccga
      181 taattcccat aaatgaaggt aatattcatt tctacactta tacacagtcc cttggacttc
      241 ttcatacaat tctttaatca taattttccc ctttctagat acagtctaac agattagaaa
      301 acacatttgt tccattttct ccattctgtt ccctttagct cctaaactgc acaaaaaaga
      361 gaggctccag cctctctcag ggttattttt cgtcattcat ttttgacttt acttcatcat
      421 aggatagtgc atgagattcc tcttctactg cttcaggcat ctttcctgtt tcgtaaagag
      481 ctttaatttg tgtactatcc aatgtttcgt atttcaataa tgcttctgca attaacttgt
      541 gagtttcacg atttgactga ataatttcag cagctttatt tcgtgcctca tttaataatg
      601 aacgaacctc ttcatcaatt tcataagctg tttgttctga aattgatttt tgaggattct
      661 gtgcaccaaa catagcatgg tttccttcat attgtactgg gccaagtttt tcactcatac
      721 cgtactctgt aaccattgca cgtgccattt gtgtcgcttg ttcaaagtcg tttgaagctc
      781 ctgtagtttg gacattaaag ataatttctt cagctacacg tccacccatt aagccagcca
      841 attgctcttt catatcttct ttagatagaa gcatttgatc ctctttagga agtgcaatca
      901 tgtatccgcc tgcacggccg cgtggtacaa ttgtaacctt atggacaacg cgagcattcg
      961 acaagactag accaacaatg gtatgtcctg cctcatggta agcaaccaat tctcgttctt
     1021 tttgtgaaac tgtcttatct ttcttagaag gtccagcaat aactctatct tctgcttcat
     1081 caatatctga agcatcaatt atcgatttat tgcgacgagc agcaactaaa gctgcttcat
     1141 tcaagacatt ctctaaatca gcaccaacaa agcctggagt ttgttgagcc actaatttca
     1201 aatcaacatc ttctgctaaa ggcttgttct tagcgtgaac tttcaagatt gcttcacgac
     1261 ctttaacatc aggacggcca accaatactt ttctatcaaa acgtcctgga cgcaaaaggg
     1321 caggatcaag tacatctgaa cggtttgtcg cagcgatgac gataatccct tcatttccct
     1381 caaaaccatc catctcaatc aaaagttggt tcaaggtttg ttcacgttcg tcattacctc
     1441 cgccgagacc aactccacgt tgacgtccaa cagcatcaat ttcatcgata aagatgatag
     1501 ctggtgctgc ttttttggca tcctcaaaaa gagagcgaac acgactagct ccaactccga
     1561 caaacatttc tacaaagtca gaacctgaga tactaaagaa tggaacacct gcttctccag
     1621 cgactgcctt agcaagcaaa gtcttacctg tccccggagg tccctccaaa agaacacctg
     1681 ctggaatacg ggctccaagt tttgtgaatc gttttggatc ttttaagaac tcaacaactt
     1741 caactagttc ttgtttttct tcctcagctc cagcaacatc tgaaaatctt actttaatat
     1801 cttctttatt tgctgcttta gccttactac gtccaaaact cattggatta cggccattgc
     1861 ctcctcccat atttcccatc atagagaata ggaagaagaa tagaattcca aatggcacaa
     1921 tggatacgag tagattaatc catataccac ttgaactttc atgcttaaca gttacttctg
     1981 ctttatggtc agtagcaagt ttttgcaatt ctgatacggt agtatctgca ggaagaatag
     2041 tgctggtaaa tttctctacc ttagtaacag atggcgtgaa aaactgaata cctgttcctt
     2101 ctttacttgt tttaggattt ttatagacac cagaaacttc gataacacta ccatttggtt
     2161 ggtaagttaa ttcttttaca ttaccatcgg taatttcttg taccaactca gtatagttga
     2221 tttgctgact tcctcctgag ttattcccag aatagaaata ctggaatcct gtcacaag
//
LOCUS       DAVMEF010000195         2272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVMEF010000195 DAVMEF010000000
VERSION     DAVMEF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.195"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            143..892
                     /gene="truA"
                     /locus_tag="VYQ30_001886"
     CDS             143..892
                     /gene="truA"
                     /locus_tag="VYQ30_001886"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1966942.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            882..1655
                     /locus_tag="VYQ30_001887"
     CDS             882..1655
                     /locus_tag="VYQ30_001887"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1966943.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            1633..2094
                     /locus_tag="VYQ30_001888"
     CDS             1633..2094
                     /locus_tag="VYQ30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1966944.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT          636 a          432 c          519 g          685 t
ORIGIN      
        1 ttgctcaaaa tagtgtgttg aggttgcaga tggaaactga cgtggtttga agaaattttc
       61 gaagagtatg agatttttat acccatacaa tatttgtttt aggtccttgt ttacagacta
      121 atttcttgat aaaatagagg ttatgacgag atataaagca actatttcct atgatggtta
      181 tgcctttgct ggctttcagc gccagcctca tgcgcgtagc gttcaggaag aaattgaaaa
      241 aaccttgacc agattaaata aagggcaaac cattactgtt cacggtgctg gtaggacaga
      301 tagtggggtt catgccctgg gacaggtcat tcattttgac ctgccttatc agatggatga
      361 ggagaaactc cgttttgcct tggataccca gtctcctgaa gatattgatg tgatttcgat
      421 tgagcttgtg gcagatgatt ttcattgccg ttatgccaaa catagcaaga cctatgagtt
      481 tattttggat agaggacgtc ccaaaaatcc tatgcgccgt cactatgcca cccactttcc
      541 ctacccactc gatgtggaac gaatgcagat tgcaatcaaa aagctagagg gaacccatga
      601 ttttaccggt tttacagcct ctgggactag tgtagaggat aaggttcgca ccatcacaga
      661 agctagttta atagtcgatg agacaggaca atttttgacc tttacctttt caggaaatgg
      721 tttcttgtat aaacagattc gcaatatggt ggggactctt ctcaaaatcg gtaacaaccg
      781 catgccagta gagcagattg atctcatctt ggagaagaag gacaggcaac ttgcaggtcc
      841 cactgcagca ccaaatggtt tgtatttaaa ggagattcgt tatgaagaat aatcgtattt
      901 tagcactttc tggaaatgat atttttagtg gtggtggact gtcagctgat ttggctacct
      961 ataccttgaa cggcttgcat gggtttgtag cagtgacttg tttgacagcc ttgacagaaa
     1021 aaggatttga agtctttcca actgatgata ccatttttca acatgaatta gatagcttgc
     1081 gtgatgtgga atttggggga attaagattg gtcttctccc tactgtcagt gtggctgaga
     1141 aggccttgga ctttatcaaa caacgcccag gagtacctgt ggtgttggat cctgtcttgg
     1201 tctgcaagga aacgcatgat gtagctgtca gtgagctctg ccaagagttg attcgctttt
     1261 tcccttatgt cagtgtgatt acgcctaatc tcccagaagc agaattatta tccggtcagg
     1321 aaattaaaac cttggaagac atgaaaactg cagcgcagaa attgcatgat ttaggagcgc
     1381 cagcagtcat tatcaaggga ggcaatcgtc ttagtcagga taaggctgtg gatgtctttt
     1441 atgatggaca gacctttact atcctagaaa atccagttat ccaaggccaa aatgctggtg
     1501 caggttgtac ctttgcctct agcattgcca gtcacttgat taaaggtgat aaacttttgc
     1561 cagcagtaga aagctctaag gctttcgttt atcgtgctat tgcacaagca gatcagtatg
     1621 gagtaagaca atatgaagca aacaaaaaca actaaaatcg cccttgtatc cctattaacc
     1681 gccctttctg tggttctagg ttatttctta aaaatcccaa cacctacagg aattctaact
     1741 cttttagatg ctggtgtctt ctttgcggcc ttttactttg gtagtcgtga aggagcggta
     1801 gtcggaggac tagcaagttt cttgattgac ctcttatcag gctaccctca gtggatgttc
     1861 tttagcttgg tcaaccatgg cttgcaggga tttttcgcag gatttaaagg aaaaagtcag
     1921 tggttaggcc ttattttagc aactattgcc atggtaggag gctacgcctt gggttctact
     1981 ttgatgaatg gctgggcagc agccctccca gaaattctac caaatttcat gcaaaatatg
     2041 gtagggatga ttgtaggatt tattcttagt caaagtatca agaagattaa gtaatactct
     2101 tcgaaaatct cttcaaacca cgtcagcttc catctgcaac ctcaaaacac tgttttgagc
     2161 agcctgaggc tagctttcta gtttgcactt tgattttcat tgagtataaa gaggttgagt
     2221 aaaaagtatt tcaaataaga aaaacgcata gtatcaggtg ttgaatgaac tg
//
LOCUS       DAVMEF010000196         2258 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVMEF010000196 DAVMEF010000000
VERSION     DAVMEF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.196"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            38..1357
                     /locus_tag="VYQ30_001889"
     CDS             38..1357
                     /locus_tag="VYQ30_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1966945.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIV
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYI
                     QRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNS
                     TGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEE
                     KASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1414..2211
                     /gene="psrA"
                     /locus_tag="VYQ30_001890"
     CDS             1414..2211
                     /gene="psrA"
                     /locus_tag="VYQ30_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1966946.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          807 a          318 c          409 g          724 t
ORIGIN      
        1 tgatggaagc actcaagaaa ttgatgttcc ttatgatatt cctgatactt gggagtgggt
       61 gaggttttct acattggttg aaattgtcag aggtggctct ccacgaccaa tcaaagatta
      121 tcttacttct gaagtagatg gaataaattg gataaaaata ggtgatactg aaaagggtga
      181 aaagtatata aataatgtta aagaaaaaat caaaaaatca gggcttaaca aaactagatt
      241 tgtaaaaaaa ggtacatttt tgttaactaa ttctatgagt tttggtagac cttatatttt
      301 gaatgttgat ggtgcaatac acgatggatg gttggctatt tcgaactatg aaaactcatt
      361 aaataaagat tacctattct atattctttc atcaaatgta gtttattctc aatttctatc
      421 tctaattagt ggagctgttg tgaaaaactt gaatagtgat aaagttgctt ctattcttat
      481 ccctctcccc ccactatccg aacaacaacg aatagtagaa gcaatcgaat cagctttaga
      541 aaaagtagat gaatatgctg aaagttataa tagactagaa cagctagata aagaatttcc
      601 agataaacta aaaaaatcta ttcttcaata tgctatgcaa ggaaaattag ttgaacaaga
      661 cccaaatgat gaatcagtcg aagttttact tgaaaaaata cgagcagaaa aacaaaaact
      721 ctttgaagaa ggcaagatta aaaagaaaga tttggacatt tctattgttt cccaaggaga
      781 tgataactct tattatgagg aagtaccttg tgaaatacca gaaagttggg aatgggtgag
      841 gttaaacgat ataacctcct acattcaaag agggaagtct ccaaaatatt caaatattcc
      901 tatataccct gtgattgctc agaaatgtaa tcaatggtct ggcttttcta ttgacttagc
      961 aagatttatt gatcccgaaa cagttcatag ttaccagaaa gaaagattac taagggatgg
     1021 tgatttaatg tggaattcaa caggtctagg tacgttagga cgattagcta tttaccatga
     1081 aaataaaaat ccttatgcct gggctgttgc agatagccat gttacagtta taagagtctt
     1141 atcgggggtt attaattgcc atttcatcta taattttctt tcctctccta ttgttcagtc
     1201 agttattgaa gaaaaagcgt cgggtagtac aaaacaaaaa gaattattaa cgaaaactat
     1261 aaaagaatat ttaatccccc tcccacccct tcctgaacaa tccagaatcg ttgataaaat
     1321 cgaacaattt ttcgcccata ttgacgcact aatttagtat aatagtttta cgtgcggtgg
     1381 aatttctatc aaacataaga taggagtttt acgttgaaag attttgaatt acatttgaag
     1441 aaagctggtt tagctgaaaa tactgtgcgt tcgtaccttt atggtgttcg atttttcttg
     1501 gagaattacg aactcaaaat ggaagattta tttgaataca aaggatattt attagacaat
     1561 tttaaaccta aaactgtcaa tttgcgacta caaggagtaa ataaatatct ggcttttatt
     1621 ggtcatgatg atttgaaatt aaaatttgtc aaagtacagc aaaaaccgtt tttagaagat
     1681 gtcattagcc atgctgatta cctttttctt aaacgtagtt tgaaaaaaga tggtatttta
     1741 aaatggcatt ttgtcgtttg gtttttaggt gcaactgggg ctcgtgtaag tgaacttatt
     1801 aaactaaaag tagagcatgt agaaatcggt tattttgaca tttactcaaa aggtggtaaa
     1861 attcgtagac tgtacattcc taaaaaatta agaaatagtt gtcttagttg gttagaatca
     1921 gaaaatcgcc gaagtggtta tttatttttg aataaattta acgaaccaat tacagcaaga
     1981 ggagttgctc aacagttaaa aaattatgct gataaataca aaatgaatcc taaagtaatt
     2041 taccctcatt cttttaggca tttatttgct aagaattttt tagcgaagta taatgatatt
     2101 gccttgcttg cagatttgat gggacacgaa agtatagaaa ctactcgaat ttatctaagg
     2161 aaaacagcta ctgaacaaca aaatattgta gataaaattg ttaattggta aaaaataaca
     2221 ggtggtcaaa ctgactacct gctatttttg tgattatg
//
LOCUS       DAVMEF010000197         2242 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVMEF010000197 DAVMEF010000000
VERSION     DAVMEF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.197"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            63..1754
                     /locus_tag="VYQ30_001891"
     CDS             63..1754
                     /locus_tag="VYQ30_001891"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1966947.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            complement(2069..>2242)
                     /locus_tag="VYQ30_001892"
     CDS             complement(2069..>2242)
                     /locus_tag="VYQ30_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966948.1"
                     /translation="LTVEDQAYNHALSKERSKVENIFAKVKTFSTTYRNHRKRFGLRM
                     NLIAGIINHELGF"
BASE COUNT          703 a          476 c          445 g          618 t
ORIGIN      
        1 attttttctt ctttctttaa aattgtgtta aaatagtaga aaagtccaaa aaggagctct
       61 taatgaatac aaaagaattg attgctagcg aattggctag catcattgat agcctggacc
      121 aagaggctat tttaaagtta ctggaaaccc ctaaaaactc agaaatgggt gacatcgctt
      181 tccctgcttt ttctcttgcc aaagtcgaac gtaaagcacc acaaatgatt gcggctgaac
      241 tggctgaaaa aatgaacagc caagcctttg aaaaagttgt cgcaacagga ccttacgtta
      301 actttttcct tgataaatct gccatttctg ctcaagtatt gcaagctgtt acaactgaaa
      361 aagaacacta tgctgaccaa aatattggca aacaagaaaa tgttgttatc gacatgtcta
      421 gtccgaatat cgctaaacca ttttctattg gccacctgcg ttcaactgtt atcggagata
      481 gcttgtcaca tattttccaa aaaatcggtt atcaaacggt caaggtcaac catttgggag
      541 actggggtaa acaatttggg atgttgattg ttgcctacaa aaaatggggc gacgaagaag
      601 ctgtaaaagc tcatccaatc gatgaactcc ttaaactcta tgtccgcatc aacgctgaag
      661 ctgaaaatga ccctagcttg gatgaagaag cgcgcgaatg gttccgtaaa cttgaaaatg
      721 gagatgagga agctctcgct ctttggcaat ggttccgcga tgaaagttta gtggaattta
      781 accgccttta caatgaattg aaggttgaat ttgacagcta taacggagaa gccttctaca
      841 atgataagat ggatgcagtt gtagacattc tttctgaaaa aggactactt cttgaatcag
      901 aaggtgccca agttgtgaat cttgagaaat atggaattga acatccagcc ctcatcaaga
      961 agtctgatgg tgcaactctc tatatcacac gtgacttggc tgcagccctt taccgtaaaa
     1021 acgaatacca atttgctaaa tctatctatg tcgttggtca agaacaatct gcccacttta
     1081 aacagctcaa agctgtcttg caagagatgg gctacgactg gagtgacgac attactcacg
     1141 ttccttttgg tttggttaca aaggaaggga agaagctctc tactcgtaaa gggaatgtca
     1201 tcttgctaga gcctactgtt gcagaggctg ttagccgtgc caaggtccaa atcgaggcta
     1261 aaaatcctga actagaaaac aaagaccaag tagcacatgc tgttggggtt ggagccatta
     1321 aattctatga cctcaaaacc gaccgtacaa atggatacga cttcgaccta gaagctatgg
     1381 tatccttcga gggtgaaact ggaccttacg ttcaatatgc ctacgctcgt atccaatcta
     1441 tcttacgcaa agccgatttc aaaccagaaa cagctggcaa ctatagcttg aatgatactg
     1501 aaagctggga aatcattaaa ctcattcaag acttcccacg tattatcaac cgtgcggcag
     1561 ataactttga accttctatc attgctaaat ttgcaattag cctagctcaa tcctttaaca
     1621 aatactatgc acatacacgt atcttggatg aaagcccaga acgcgacagc cgtctagccc
     1681 tcagctacgc aaccgcagtc gttctaaaag aagcccttcg cttgcttgga gtagaagcgc
     1741 cagagaagat gtaaatcgcc aaagttactt gattgcaaag caatctaagt aactaacgcc
     1801 aaatcctatt cggactttgg ctaggcgctt cactagtttt aggacataaa tatgatcgat
     1861 ttatgtccta aaacgtcgta atcttaaagt tattggaact aaagttccta attgttagac
     1921 gctagccgct tatatgcgaa ctagctaact gcttcactag tcatgacccc taaatataat
     1981 cgatttagag gccatgacgt cgtaaccaga gacacttgcc taaacgcttc actaattcac
     2041 ctaaccaaat aatagacttc ctgcaaaact agaatcctag ttcatgattg ataataccag
     2101 caatcaaatt cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt gttgaaaacg
     2161 tttttacttt ggcaaagatg ttctcgacct tgcttctctc cttggatagc gcatggttat
     2221 aggcttgatc ttcaactgtt ag
//
LOCUS       DAVMEF010000198         2226 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVMEF010000198 DAVMEF010000000
VERSION     DAVMEF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.198"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..139)
                     /locus_tag="VYQ30_001893"
                     /pseudo
     CDS             complement(1..139)
                     /locus_tag="VYQ30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(488..880)
                     /gene="rpsI"
                     /locus_tag="VYQ30_001894"
     CDS             complement(488..880)
                     /gene="rpsI"
                     /locus_tag="VYQ30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1966949.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(899..1345)
                     /gene="rplM"
                     /locus_tag="VYQ30_001895"
     CDS             complement(899..1345)
                     /gene="rplM"
                     /locus_tag="VYQ30_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1966950.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISELI"
     gene            1794..>2226
                     /locus_tag="VYQ30_001896"
     CDS             1794..>2226
                     /locus_tag="VYQ30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1966951.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKR"
BASE COUNT          691 a          412 c          450 g          673 t
ORIGIN      
        1 gctttttgat atgagcccat gttttctcaa taggattgta ctcaggtgag tagggaggaa
       61 gaggttaaag tttataccca aactcttcac acaagagttc taacttaccc attctatgga
      121 atcttgcatt atccataata tagtagattg aaactagaat agtacacctc tacttctaaa
      181 acgttgttag aattcgattt gactatcctc ggtttgtcct attcttattt caccttacta
      241 taaaaacaag gtaaactttt caaaaaaaca cgaaaaaagc acccataggg taaacccttg
      301 agtgctttga aacgttgata taatcgtgtt gattaacgtt ttgaggcaac tgaccttgtc
      361 aggttgcagc cacatttgta agcgactaaa gtcgcaacaa ctgtgtcaat tgcaccaatt
      421 tagtaagaaa gtataaaaaa agaacacccc gaaaggtgct ctgtataagt atagtaattc
      481 tttcgaatta acgtttacta aattgtgatg ctttacgagc tttcttaaga cctggtttct
      541 tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc gcgtttcaat gaatcgcgga
      601 agtctgggtc tacttgaaga agggcacgag cgataccgtg acggatagct cctgattgac
      661 cagcgtatcc accacctaca acgttaacga aaacgtcgta tgaacctaca gttgaagtaa
      721 ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg tgggatgtac tcttcaacat
      781 cttttttgtt aacagtgatt ttaccagttc ctggaacaag gcgaacgcgt gcaacagcgt
      841 ttttacgacg tccagtacct gcatattgtg cttgtgacat actttattgt tcctttcctt
      901 agataagttc tgaaatgtca agaacttctg gttgttgtgc agcatgagtg tgctcagctc
      961 caacaaatac tttcaacttc ataccttgag cgcgtccaag tgtattgtgt ggaagcatac
     1021 ctttaactga tttctcaatc aaacgtactg catttttaga acgaagttca cctgcagaga
     1081 tttgtttcaa tccacctggg tggtttgagt gagtgtagta gattttatca gttgcttttt
     1141 taccagtcaa tttaactttt tcagcattga taacaatcac gaagtcacct gtatcagtgt
     1201 gtggtgtaaa tgttggtttg ttttttccgc gaagtacgct agcaactact gcagaaagac
     1261 gtccaagtgg tacatcagtt gcgtcaacta cgtaccattt acgttcaact tggcctggtt
     1321 tagccataaa tgttgttttg ttcatgattt ctcctatata tagttcgatt tttgtttacg
     1381 gtgatgggtg ttccttccca tcgaaaagta tttggaaggt tccggggcct ttcaaatggg
     1441 gtaaacaata ccgcctacta tcataccaaa tttcacccct aaaagtcaat agatatgaaa
     1501 aatatttttc ggtaacttct caaagtaact gaaatgaaag ttctataatg aagttagcca
     1561 ccttacctta gtcaagaatt agatgtttca ctatgtttga gtaagttgat gatttcattt
     1621 gataacaggt ttgaaactgt taggctaggt ggccaaggct aatcatagcc ttggtttagc
     1681 tgaaaaaaca ggttcaaggg ttcctgttgt caaatgaaat gtgatttaag gtataagaaa
     1741 acacctctgt gctatacttg ttgttcacca caaacacaag gaaaggcaca gagatgcaag
     1801 aacattatac cccaaaaggg aaacatttga caatagataa ccgtcgcttg attgagcggt
     1861 ggaagaatga aaataagtcc aatcgtgaaa ttgcaggctt gttaggaaag gcgcctcaaa
     1921 cgattcataa tgaagtcaaa agaggtacaa ctttacaaca agtgagaaaa gggctataca
     1981 aaaaggtcta ttctgccgat tacgcacaaa ctgtttacca attcaatcga aaacggtcgg
     2041 tgaaaaagtt aattttaaca aaggaaatca gagagaagat cttacactat cataagcaaa
     2101 aattttcgcc tgaaatgatg gttaacaaga agcaagtgaa agttggtatt tcaaccatct
     2161 actactggtt tcataatggt catttaggat tgacgaaggc cgacatgctt tatcccagaa
     2221 aaagga
//
LOCUS       DAVMEF010000199         2206 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVMEF010000199 DAVMEF010000000
VERSION     DAVMEF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.199"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            166..1884
                     /locus_tag="VYQ30_001897"
     CDS             166..1884
                     /locus_tag="VYQ30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1966952.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            complement(1934..>2206)
                     /locus_tag="VYQ30_001898"
     CDS             complement(1934..>2206)
                     /locus_tag="VYQ30_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966953.1"
                     /translation="EKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAK
                     LEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT          678 a          446 c          458 g          624 t
ORIGIN      
        1 tttttttact gaaattgaat ttttcctagc ctaaacatca tataacccca aaattcaatc
       61 tgacctggtt tgaagagatt ttcgaagcag ataaggtttt actaaaacta aaaaaaatga
      121 tataatcaaa ctgattaaat gttttaaaaa taaaggagaa tttgaatgtc ttaccaagaa
      181 aattaccaga aatgggttga ttttgtggag cttcctgact accttcgtca agatttggaa
      241 aatatggacg aaaaaactaa ggaagatgcc ttctatacaa atcttgaatt tggtactgca
      301 ggtatgcgtg gcttggttgg tgctggtaca aaccgtatca acatctacgt tgttcgccaa
      361 gctactgaag gattggctcg tttgattgag tcaaaaggtg gaaacgagaa agaacgcggt
      421 gtagcaattg cctacgatag ccgtcacttc tcacctgagt ttgcctttga atctgcggca
      481 gttcttgcta aacacggcat caaatcttac gtatttgaaa gccttcgtcc aactccagaa
      541 ctatcatttg cagttcgtca tctcaactgt ttcgcaggta tcatggtcac agccagccac
      601 aaccctgctc catttaacgg ttacaaggtt tacggtgaag acggtggaca aatgcctcca
      661 cacgatgcgg acgctttgac tacttatatc cgtgcaatcg aaaacccatt tgcagttgaa
      721 gttgctgatg tggaaactga aaaagcttct ggcttgattg aagttatcgg cgaagctgtt
      781 gacatagaat accttaaaga ggttaaggac ataaacatca acccagcctt gattgaagaa
      841 tttggtaaag acatgaagat tgtctacaca ccacttcatg gtactggtga aatgttggct
      901 cgtcgtgctc ttgcccaagc aggatttgac tctgttcaag ttgttgaagc gcaagcaact
      961 gctgacccag acttctcaac tgtaacatct ccaaacccag aaagccaagc agcctttgcc
     1021 cttgctgaag aacttggtcg tcaagttggt gcagatgttc ttgtcgcaac tgacccagac
     1081 gctgaccgtg ttggtgttga agttcttcaa aaagatggta gctacctcaa cctttcaggt
     1141 aaccaaatcg gtgctatcat ggctaaatac atcttggaag ctcacaaaaa cgctggaact
     1201 cttcctgaaa atgccgctct ctgcaaatct atcgtttcaa ctgacttggt aacgaagatt
     1261 gctgaaagct acggcgcaac tatgttcaac gtcttgacag gtttcaaatt tatcgctgaa
     1321 aaaatccaag aattcgaaga aaaacacaac cacacttaca tgatgggatt tgaagaaagc
     1381 ttcggttact tgattaaacc atttgtacgt gataaagatg ccatccaagc cgttcttgtc
     1441 gttgctgaac ttgctgccta ctaccgttct cgtggtttga cacttgctga cggtatcgaa
     1501 gaaatctata aagaatatgg ctactacgca gaaaaaacaa tctctgttac tctttcaggt
     1561 gtcgatggtg ctgaacaaat caaagcgatt atggctaaat tccgcaacaa tgctccaaca
     1621 gaatggaacg caacagctat cactgtcgta gaagacttca aggcacaaac tgctactgtt
     1681 gctgacggta ctgttacaaa cttgacaact cctccaagtg atgtgttgaa atacacactt
     1741 gctgacggtt catggattgc cgttcgccct tcaggtacag aaccaaaaat caagttctac
     1801 attgcagttg taggggaaac caacgaagaa tcacaagcta agattgctaa catcgaagca
     1861 gaaatcaatg catttgtaaa ataaagacct ataaaagatg gctttttgtc aactgtagtg
     1921 ggttgaaaaa aagctaagct tgagaaagga caaatttcgt cctttctttt ttgatattca
     1981 gggcgataaa aatccgtttt ttgaagtttt caaagtttcg aaaaccaaag gcattgcgct
     2041 tgataagttt gatgagatta ttggtcgctt ccaatttggc gtttgaatag ggtagttgaa
     2101 gggcgttgac gattttctct ttgtccttta gaaaggtttt aaagacagtc tgaaaaagag
     2161 gatgaacctg cttaagattg tcctcaataa gtccgaaaaa tttctc
//
LOCUS       DAVMEF010000200         2205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVMEF010000200 DAVMEF010000000
VERSION     DAVMEF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.200"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            96..2078
                     /locus_tag="VYQ30_001899"
     CDS             96..2078
                     /locus_tag="VYQ30_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1966954.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKIGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT          754 a          390 c          458 g          603 t
ORIGIN      
        1 agaatttacc aaattaatta aaaaattcag aaaattatat tgacatctct ctgaaaagag
       61 tctataatag agagaaagtt ttaaaggaga agatgatgaa aagttcaaaa ctatttgccc
      121 ttgcgggtgt aaccttattg gcggcgacta ctttagctgc atgctctgga tcaggttcaa
      181 gcactaaagc tgagaagaca ttctcataca tttatgagac agaccctgat aacctcaact
      241 atttgacaac tgctaaggct gcgacagcaa atattaccag taacgtggtt gatggtttgc
      301 tagaaaatga tcgctacggg aactttgtgc cgtctatggc tgaggattgg tctgtatcca
      361 aggatggatt gacttacact tatactatcc gtaaggatgc aaaatggtat acttctgaag
      421 gtgaagaata cgcggcagtc aaagctcaag actttgtaac aggactaaaa tatgctgctg
      481 ataaaaaatc agatgctctt taccttgttc aagaatcaat caaagggttg gatgcctatg
      541 taaaagggga aatcaaagat ttctcacaag taggaattaa ggttctggat gatcagacag
      601 ttcagtacac tttgaacaaa ccagaaagct tctggaattc taagacaacc atgggtgtgc
      661 ttgcgccagt taatgaagag tttttgaact caaaagggga tgattttgcc aaagcgacgg
      721 atccaagtag tctcttgtat aatggacctt atttgttgaa atccattgtg acaaaatctt
      781 ctgttgaatt tgcgaaaaat ccgaactact gggataagga caatgtgcat gttgacaaag
      841 ttaaattgtc attctgggat ggtcaagata ccagcaaacc tgcagaaaac tttaaagatg
      901 gtagccttac agcagctcgt ctctatccaa caagtgcaag tttcgcagag cttgagaaga
      961 gtatgaagga caatattgtc tatactcaac aagactctat tacgtatcta gtcggtacaa
     1021 atattgaccg tcagtcctat aaatacacat ctaagaccag cgatgaacaa aaggcatcga
     1081 ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat tgcctttggt tttgatcgta
     1141 cagcctatgc ctctcagttg aatggacaaa ctggagcaag caaaatctta cgtaatatct
     1201 ttgttccacc aacatttgtt caagcagatg gtaaaaactt tggcgatatg gtcaaagaga
     1261 aattggtcac ttatggggat gaatggaagg atgttaatct tgcagattct caggatggtc
     1321 tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc ttacaagcag
     1381 aaggtgtgac attcccaatt catttggata tgccagttga ccaaacagca actacaaaag
     1441 ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac tttaggagct gataatgtca
     1501 ttattgatat tcaacaacta caaaaagacg aagtaaacaa tattacatat tttgctgaaa
     1561 atgctgctgg cgaagactgg gatttatcag ataatgtcgg ttggggtcca gactttgccg
     1621 atccatcaac ttaccttgat atcatcaaac catctgtagg agaaagtact aaaacatatt
     1681 tagggtttga ctcaggggaa gataacgtag ctgctaaaaa aataggtcta tatgactacg
     1741 aaaaattggt tactgaggct ggtgatgagg ctacagatgt tgctaaacgc tatgataaat
     1801 acgctgcagc ccaagcttgg ttgacagata gtgctttgat tattccaact acatctcgta
     1861 cagggcgtcc aatcttgtct aagatggtac catttacaat accatttgca ttgtcaggaa
     1921 ataaaggtac aagtgaacca gtcttgtata aatacttgga acttcaagac aaggcagtca
     1981 ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa gagtctaata
     2041 aaaaggctca agaagatctc gcaaaacatg tgaaataact gttgcaaaat ataagaaagg
     2101 atttagtatt tctcttgaat gctgaatcct tttttacatt tgtaaagaaa gcttctaaat
     2161 gtactgaccc ccaaaagttg gagcctcttt ttgtcagaat agaga
//
LOCUS       DAVMEF010000201         2170 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVMEF010000201 DAVMEF010000000
VERSION     DAVMEF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.201"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            129..1622
                     /locus_tag="VYQ30_001900"
     CDS             129..1622
                     /locus_tag="VYQ30_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1966955.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2170
                     /locus_tag="VYQ30_001901"
     CDS             1635..>2170
                     /locus_tag="VYQ30_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1966956.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFG"
BASE COUNT          762 a          326 c          430 g          652 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcta
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggaa
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctaatagca tgggatggtg ctaatgcagg aacagttggc tatgggttat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagatcatt
     1981 caacaggtgc aactattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaatttcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
     2161 agatgtttgg
//
LOCUS       DAVMEF010000202         2169 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVMEF010000202 DAVMEF010000000
VERSION     DAVMEF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.202"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..239
                     /locus_tag="VYQ30_001902"
                     /pseudo
     CDS             105..239
                     /locus_tag="VYQ30_001902"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(477..1909)
                     /locus_tag="VYQ30_001903"
                     /pseudo
     CDS             complement(477..1909)
                     /locus_tag="VYQ30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          669 a          425 c          390 g          685 t
ORIGIN      
        1 aaattgttgg ttaggcaatg ggaaatcgaa tgcaagataa gttggtaacg aatgttttca
       61 atcaagctta tcatagagaa aatctcaaat aagaactagt cggccatacc gatcaaggat
      121 ctcaatatac tggagctaga tttcaagaac tccttaaaca gaaaagatgc aagtctagcc
      181 tgagtcgaaa aggcaatcca tatgataatg cacccatgga atccttttac aaaacataaa
      241 aagagaactt gtcaatgatg ccaatttcgc aactattgaa cgaaatcttt aaatatattg
      301 aaatctatta caatcctaaa tatctccatt cggcattagg gtaccaataa ctaatagagt
      361 ttgaaaaaaa tagtcactaa tcatcgagtc cactgaaaaa gtaagctcta cagttcaact
      421 aaaatgaaaa aagagggatc tatctcctgc ttggatagat tcctctttgc ttttccttat
      481 ttttggttta ttagagttat aatcctcttc atattaacca caaatatggt tgtggcggct
      541 tgtaactcca tgtttaaaag acctggtgct ctagctacat taagaccatg tctgttttca
      601 agttccgcat ttttggcttc tatcttgtag cgatgtttgg ccttttcttt aaaatagggc
      661 gcttcctgaa acctcttctg gaaaatatgt tcatcgctct taaggggtat caaataagtc
      721 ttagacttag ccccttcttt ataacagccc acttttgaag ggcatatttg acagtgcttg
      781 atgtcaaaat agtgggtttc ctgagccgtc cttcttcctg tcttttcctt ctttagtgcc
      841 atatgccctt ctggacaaac aaagagacct gcgtccttgt tatagaaaaa ctcatcttcc
      901 ttcctacgaa agccttttga aatagttgga tatggcctag cgactcaatg cacacgttct
      961 ttcttggtaa attgcatatt atccttccca gagtaggccc tatctccaac aatagcttca
     1021 atcgtcacgc cattatcctt agatttgtga tagagttctt ctaaaacagg accatcactt
     1081 ttttcacccg atgtcaccac acaggcagta ataatccgtt catcggtcat ggcaaaatgc
     1141 tctttgtacc cgtaaaagga actatcggct gacttatgtc caattcttgc ttcttctttg
     1201 acagaggatt ccaagtgctc taagtcatcc tctacagctt ctttaaggta attgaatttt
     1261 gtgagatagc tggtattcca gtcaattcgt catgcttctc aaggactgat attacttctt
     1321 gtgtatatct taattcagct actagagtat cttcctgagg cttactagga aattctgcct
     1381 tgatatattc cgaatactga taaatcgttt tacgcaaggc ttttgagcgt tctcggagga
     1441 cttcttgagg tttcttgtga ttgtaatgaa ctttggtatg agtcgcatcc acaatgagaa
     1501 tgttactttt aatcagaccg agctctatcg caatctgaac agatttagcg atgagcaaat
     1561 caagtaatcg ttcatctttg ttccgaagtt ttcggaattt agtcaatgag gacggttcaa
     1621 taacaggagc tagaccaagg aagtatttga aggtcatacc agaaaaggag cgctccacca
     1681 catccacatc ggacaactta tagatgtctt ttaataagag atacttgaac atcatgataa
     1741 ggagagagta ggaacgacat ccgaagtcag gttgataatt cttttccagc tcgtcataaa
     1801 taaaactaaa atcacagagt tccattagct gtcgtaaaaa atgagttttg ggaacaacaa
     1861 tatcacacag agtagaatag ggactaacat cgattatcaa gcataccatc acctcatctt
     1921 tagtatacca tcatacgatg actttttcag tggactccga attatcttaa ctcttgacaa
     1981 gtccagtgat gcaatgatag agcatagaca actttaatga tttggggtcg aagcgtaatc
     2041 gtaaaatagg taaaatcgtc tttgaatgca tttttattaa tgtttgtttt agaagttaaa
     2101 ccgatattgg caaccataaa cagaaaagat atccaaaaaa atttgagttt gtcttcagtc
     2161 tgatttata
//
LOCUS       DAVMEF010000203         2157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVMEF010000203 DAVMEF010000000
VERSION     DAVMEF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.203"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            8..2113
                     /locus_tag="VYQ30_001904"
     CDS             8..2113
                     /locus_tag="VYQ30_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1966957.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKHKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFNILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLNPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT          671 a          420 c          507 g          559 t
ORIGIN      
        1 aaagaatatg aataacaact ttaataattt taataacatg gatgatttat ttaaccaatt
       61 gatgggtggt atgcgaggat atagttctga aaatcgccgt tacttaatta atggacgcga
      121 agtcacacct gaggaatttg ctcactatcg tgcgactggt caattaccag gaaatgcaga
      181 aactgatgtg caaatgccac aacaggcatc aggtatgaaa caagacggtg tccttgcaaa
      241 actaggtcga aacttgacag cagaagcgcg tgagggcaag ttggatcctg ttatcggacg
      301 aaacaaggaa attcaagaaa catctgaaat cctctcacgc cgcaccaaga acaatcctgt
      361 tttggtcgga gatgcaggtg ttggtaagac agcagttgtc gaaggtctag cgcaagccat
      421 tgtgaacgga gatgttcctg ctgctatcaa gcacaaggaa attatttcta ttgatatctc
      481 aggtcttgag gctggtactc aataccgtgg tagctttgaa gaaaatgtcc aaaacttagt
      541 caatgaagtg aaagaagcag ggaatattat cctcttcttt gatgaaattc accaaattct
      601 tggtgctggt agcactggtg gagacagtgg ttctaaaggg cttgcggata ttctcaagcc
      661 agctctctct cgtggagaat tgacagtgat tggggcaaca actcaagacg aataccgtaa
      721 caccatcttg aagaatgctg ctcttgctcg tcgtttcaac gaagtgaagg tcaatgctcc
      781 ttcggcagag aatactttta acattcttca aggcattcgt gacctctatc aacaacacca
      841 caatgtcatc ttgccagacg aagtcttgaa agcagcggtg gattattctg ttcaatacat
      901 tcctcaacgt agcttgccag ataaggctat tgaccttgtc gatgtaacgg ctgctcactt
      961 ggcggctcaa catccagtaa cagatgtgca tgctgttgaa cgagaaatcg aaacggaaaa
     1021 agacaagcaa gaaaaagcag ttgaagcaga agattttgaa gcagctctaa actataaaac
     1081 acgcattgca gaattggaaa agaaaatcga aaaccacaca gaagatatga aagtgactgc
     1141 aagtgtcaac gatgtggctg aatctgtgga acgaatgaca ggtatcccag tatcgcaaat
     1201 gggagcttca gatatcgaac gtttgaaaga tatggctcat cgcttgcaag acaaggtgat
     1261 tggtcaagat aaggctgtag aagctgtagc tcgtgctatc cgtcgtaacc gtgctggttt
     1321 tgatgaagga aatcgcccaa tcggcagctt cctctttgta gggtctactg gggttggtaa
     1381 gacggagctt gctaagcaat tggcactcga tatgtttgga acccaggatg cgattatccg
     1441 tttggatatg tctgaataca gtgaccgcac agctgtttct aagctaattg gtacaacagc
     1501 aggctatgtg ggttatgatg acaatagcaa taccttaaca gaacgtgttc gtcgcaatcc
     1561 atactctatc attctcttgg atgaaattga aaaggctgac cctcaagtta ttacccttct
     1621 cctccaagtt ctagatgatg gtcgtttgac agatggtcaa ggaaatacag taaacttcaa
     1681 gaacactgtc attattgcga cctcaaatgc tggatttggc tatgaagcca acttgacaga
     1741 agatgcggat aaaccagaat tgatggaccg tttgaacccc ttcttccgtc cagaattcct
     1801 caaccgcttt aatgcagtca tcgagttctc acacttgact aaggaagacc tttctaagat
     1861 tgtagatttg atgttggctg aagttaacca aaccttggct aagaaagaca ttgacttggt
     1921 agtcagtcaa gcggctaaag attatatcac agaagaaggt tacgacgaag tcatgggggt
     1981 tcgtcctctc cgtcgcgtgg ttgaacaaga aattcgtgat aaggtgacag acttccactt
     2041 agatcattta gatgctaaac atctggaagc agatatggaa gatggcgttt tggttattcg
     2101 tgagaaagcc taagatagaa ttttgaggat aaaaaagaag gagccagctg aaaaaaa
//
LOCUS       DAVMEF010000204         2155 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVMEF010000204 DAVMEF010000000
VERSION     DAVMEF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.204"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            49..751
                     /locus_tag="VYQ30_001905"
                     /pseudo
     CDS             49..751
                     /locus_tag="VYQ30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(898..1743)
                     /locus_tag="VYQ30_001906"
     CDS             complement(898..1743)
                     /locus_tag="VYQ30_001906"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1966958.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1999..>2155
                     /locus_tag="VYQ30_001907"
     CDS             1999..>2155
                     /locus_tag="VYQ30_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1966959.1"
                     /translation="MLYVGIDVAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDC"
BASE COUNT          714 a          439 c          428 g          574 t
ORIGIN      
        1 ctgacgtggt ttgaagagat tttcgaagag tataaaaagg tgctaggcat gttgattttt
       61 cctttgttaa atgatttgtc aagaaaaatc atccatattg acatggatgc cttttttgct
      121 gcagtggaaa tcagggataa tcctaaactc agaggaaaac ctgtcattat tggaagcgac
      181 cctcggcaaa caggtggacg gggagtcgtt tctacctgta gttatgaggc aagagctttt
      241 ggtgtccatt ctgccatgag ttccaaggaa gcttatgaac gttgtcccca ggctgtcttt
      301 atctcaggga attatgagaa atacaagtct gtgggactcc agattcgagc tatttttaag
      361 cgctatacag attggtagac taggctatga tctgtatcga aaggctcgtg gcattcacaa
      421 ctctccagtc aaatccaatc gcatccgtaa atcaatcggc aaggagaaaa cctacgggaa
      481 gattctccgt gctgaggaag atatcaaaaa agagctgact cttctatcag aaaaagtcgc
      541 tctcaatcta catcaacaag aaaaagctgg aaaaattgtc attttgaaaa tccgctacga
      601 ggacttttca actcttacca aacgaaaaag tcttgctcaa aaaacacaag atgctagtca
      661 gataagccaa atagccctgc aactctatga agaattaagc gagaaagaaa gaggtgtccg
      721 cctattgggg attaccctga ctggatttta aagctcaatg aaaatcaaag agcaaactag
      781 gaagctagcc gcaggctgct caaaacactg ttttgaggtt gcagataaag ctgacgcggt
      841 ttgaagagat tttctaagag tataaaacct tgaagagttg ccccttcaag gtttttctta
      901 tacaaataaa cggacaaaac tgtaaagtag caaaacacta ccaagcacga tacggtattt
      961 accaaaaagg gtgaagtcgt gttttttcac atagctggtc aagaagcgaa tagccaccat
     1021 gctgaccgca aaagctactc ccatcgcgac caagagcaaa aacaattgcc caaagctcaa
     1081 gagttctccg gctttcacaa atttgaaaat ctttaaggca ctagctccaa acataacagg
     1141 aatcccaaga tagaaggtaa attctgtcac aactgaacga ctggttccat ttaacaaacc
     1201 accgacaatc gttgcacctg aacggctagt ccctggtaaa agagcaagaa cttggaagag
     1261 tccgatatag aaagcggtcg tataaggaag cttgtccaac tctgttacac ttggctcgat
     1321 agcacgcgct ttattgcgct tttccaaata gatgaaggca accccgtaga taatcaacat
     1381 gagagcaact gaaaccatgt tatggaagtg ggtatcaaac caatcatcaa atttaaagac
     1441 accaagtaaa ggcaaagtgg caatcaagac cttcaaccat agtctccaag tcttacgaac
     1501 ttcctgtttg tccttagtcg gtttaaaagg attgagcttg ttaaaataaa tcaccataac
     1561 tgctaaaata gcaccaagct gaattacgac attaaacatg gacataaagg cttcattttg
     1621 attttggtat tggataaact cctctgctaa aatcaagtga cctgtactgg aaatcggcaa
     1681 ccattccgta attccttcaa caatcccgaa gaagatagat tttaaaattt caataagata
     1741 catagatttc tccttatatt atatttaacg cagttagcta ctgccaaagc ccaagtggta
     1801 tacttggaat aagccactgt ggattagttc atttcctcca atcgcttgac gatttccgaa
     1861 agggtctaac cacagctttt tgtttcaatt ggtaatgagt ttgataacgc atagacgttt
     1921 tatctcgtct aaggtaacaa ggcaaaaagt tcgtggataa ccaagtagac ttgaaatact
     1981 atatctggag gtactcccat gctttatgtg ggcattgatg tcgctaaaaa taaacacgat
     2041 gttacagcct tgaatgttca aggaaaaact gttcttaaac cactcacttt ttcaaataat
     2101 aaagctggtt ttgaactctt agatctgtct tttcgacagc tcaaccaaga ctgtc
//
LOCUS       DAVMEF010000205         2129 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVMEF010000205 DAVMEF010000000
VERSION     DAVMEF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.205"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..876)
                     /locus_tag="VYQ30_001908"
     CDS             complement(<1..876)
                     /locus_tag="VYQ30_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1966960.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRL"
     gene            complement(879..>2129)
                     /locus_tag="VYQ30_001909"
     CDS             complement(879..>2129)
                     /locus_tag="VYQ30_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1966961.1"
                     /translation="LQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMVIASF
                     PIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGIVSLS
                     SFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMPDGVP
                     PAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVIGLL
                     ILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFMDNFV
                     FIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVLNIL
                     LLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQIVL
                     IVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT          742 a          485 c          298 g          604 t
ORIGIN      
        1 caagcgagat actgtatgtc tcactcctct aattgccttg ctcaatgcat aaatattatc
       61 gactgttgcc agtcccccat atcctgcgtc acctatatgt tgaatatcaa ctccacaaat
      121 tttatttcta agtgcaattt ccttgatagt atctgtatca gatgtttctt ggctagtacc
      181 aatagcactc aatacaagac cacccttact atgaacgaga tcaacgactt cacgcaactc
      241 ttggtcatga aaggctggaa cggttccaac tgctggaaca agtatcacat ccgcacctgc
      301 ttccaacaat tgctctgcga cagaaagctc tgccacaggc tcattcactc ctgccccgtg
      361 catcttacct gcaataatca aaccagaaaa gttttcctta gcagtttgaa cagccttaat
      421 gatttctcga ttgctaactc ctgttccagg atttccagtc aaacagacaa agtcaaaacc
      481 taactcctct attcgattca atgtttcaac actagcaaca cgacctgcaa caatttcctg
      541 tgtttcctct agcatctttg cagatgggtc aatcggttcc aaatttacac caattggaca
      601 agcaacaagc tggtgtaagc gatgaataac atcatcacct gaactatcca aagcataaat
      661 tttaggctca aaaacatcaa cacaattcaa taaaatcata tcagctccaa aagctctagc
      721 aatttcagaa ttagttatat ccccaacaaa tgtctcacga gtaactacat tttcagatag
      781 aacaactcta ccttcacttg ctaaaatact ttgttttaat tcttcagcat tcatctgtaa
      841 tatctcagat ggatttgcac taattaaacg tttcattctc aatcctccaa taacttaaac
      901 cgtttttcaa tagctattac aaatggtaaa taaagtaaga catccaaaac aatcaataca
      961 atttgtaata atgaaccaga aatacttcct gtagcaagta aaccacttat aacaggtggc
     1021 atagtccaac ctggatctgt ataagttagc ggaaccaaac ctgatgccat tgctccccac
     1081 gctactagta agttaaacat tggagctaag ataaatggaa ctagtaacaa aatgttcaaa
     1141 acaataggaa cgccaaacat tgctggttca tttatattaa ataatccagg tataacagtt
     1201 ataggtgcca aagtttttag ttgtttacta gctttcttct tatgtgctaa atatcctaac
     1261 gcaagaacta aacctatggt tgcaccaccg ccacctatga atacaaaatt atccataaat
     1321 acgctagtaa aaatatttgg aagagtttct ccagtttgat aagctacttt atttgcatct
     1381 agattagcta accataacgg tttcataact gcattaacaa catttcctcc gtgtaatcct
     1441 acaaaccaaa aactactatt aaggaaaatt aagataagaa gaccaataac attatttcct
     1501 aaaagtccaa gtggacctcc caaagctact tgagcaattt catgaagatt aggtaaactt
     1561 aatttatcta aagttgcata aacaatcaac caaccaacaa tagtcactgc accaggaata
     1621 atggcactaa agcttttaga tactgctggt ggaacaccat ccggcatttt tatctgtata
     1681 ttatgattta taaaccattg gtagatatag ccattaatca atcctaaaat catagcaaca
     1741 aataaacctt ttgatgccat gaaagcggta ggcattcctg ctccagcctc tcctctaatg
     1801 aaaggcgtaa ctgtaataaa agatgataat gacacaatac cagcaggaat accatcaact
     1861 ttgtattgtc tagccataaa gtaagcgata ccaaaactag ctacaagacc aagtaatcca
     1921 aaactactat caacaccctt ccataaatag tcagcaacac caatatcacc cagatatttt
     1981 tcccatcctg gaataggaaa acttgcaata accattagta atgaacctat caacaataaa
     2041 ggtatagtta gagtaatacc gtctcgaata gaaaccaacg ctttattatt acccaatcta
     2101 gccgctactg gaagtaactt ttcttgtaa
//
LOCUS       DAVMEF010000206         2124 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVMEF010000206 DAVMEF010000000
VERSION     DAVMEF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.206"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            63..419
                     /gene="glnR"
                     /locus_tag="VYQ30_001910"
     CDS             63..419
                     /gene="glnR"
                     /locus_tag="VYQ30_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1966962.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            456..1802
                     /gene="glnA"
                     /locus_tag="VYQ30_001911"
     CDS             456..1802
                     /gene="glnA"
                     /locus_tag="VYQ30_001911"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1966963.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(1898..2017)
                     /locus_tag="VYQ30_001912"
     CDS             complement(1898..2017)
                     /locus_tag="VYQ30_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1966964.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT          655 a          398 c          456 g          615 t
ORIGIN      
        1 acattatcaa ttgacgtttg tcttttttta gactataata gacagaaaga aggaaattgt
       61 aaatgaagga aaaagaattt cgccgaaata tggctgtttt tcctatcggc agtgttatga
      121 agttgaccga tctatcggcg cgtcagattc gttattatga agatcaagag ttgatcaagc
      181 ccgatcgaaa cgaaggaaat cgtcgcatgt attccttgaa tgacatggat cgtctgcttg
      241 aaatcaaaga ttatatctct gaaggttata atatcgctgc cattaagaaa aaatatgctg
      301 aacgtgaagc gaaatccaag aaagcggtta gtcagactga agtacgtcgt gcacttcaca
      361 atgaactcct ccaacagggg cgttttgctt cagtacagtc accttttggt cgcggttagg
      421 caaccgcaag tagtcataga ttaaggagaa aacttatgcc aatcacagct gcagatattc
      481 gtcgtgaagt caaggaaaaa aatgttacct ttattcgtct tatgttctca gatattttgg
      541 gaaccatgaa aaacgtcgaa attcctgcta cagatgaaca gttagataag gtcttgtcga
      601 acaaggttat gtttgatgga tcttctattg aaggttttgt acgtatcaat gagtcggata
      661 tgtacttgta cccggacttg gatacatgga cagtcttccc ttggggagat gaaaatggaa
      721 gtgttgcagg tctgatctgt gatgtctata caacagaagg tgaaccattt gcgggtgacc
      781 ctcgtggtaa tttgaaacga gctcttcgtc acatggaaga agttggattc aaatccttca
      841 accttggtcc agagccagaa ttcttcctat ttaagttgga tgaaaatggg gacccaacac
      901 ttgaagtgaa tgacaagggt ggctactttg atttggcacc tactgacctt gcggacaaca
      961 cacgtcgtga gattgtgaat gtcttgacca aaatgggatt tgaagtagaa gcgagtcacc
     1021 acgaggttgc ggttggacag catgagattg actttaagta cgatgaagtt ctccgtgctt
     1081 gtgataagat tcaaatcttt aagcttgttg ttaaaaccat tgctcgcaaa cacggacttt
     1141 acgcaacatt tatggcgaag ccaaaatttg gtattgctgg atcaggtatg cactgtaata
     1201 tgtccttgtt tgatgcagaa ggaaataacg ccttctttga tccaaatgat ccaaaaggaa
     1261 tgcagttgtc agaaacagct taccatttcc taggcggttt gatcaagcat gcttacaact
     1321 atactgccat catgaaccca acagttaact catacaaacg tttggttcca ggttatgaag
     1381 cgcctgttta cattgcttgg gctggtcgta accgttcgcc acttgtgcgc gtacctgctt
     1441 cacgtggtat gggaactcgt cttgagttgc gttcagtgga tccaatggcg aacccttaca
     1501 ttgctatggc tgttcttttg gaagttggtt tgtatggtat tgaaaataaa atcgaagcac
     1561 cagctcctat cgaagaaaat atctacatca tgacagcaga agagcgcaag gaagctggta
     1621 ttacagacct tccatcaact cttcacaacg ctttgaaagc tttgacagaa gatgaagtgg
     1681 ttaaagctgc tctcggagat cacatctaca ctagcttcct tgaagccaaa cgaatcgaat
     1741 gggcaagtta tgcaaccttc gtttcacaat gggaaattga taattattta gacctttact
     1801 aatactaata tagaagaaag attgcctgtg ggtaaacagt cacacagcag tctaagccat
     1861 caaataccta taacccttaa aatagcgatt tcggtcgcta tttatttttc gaaaaatcct
     1921 cttgactctt ttgcgaatta gatttatcat cagacgtgta gaagaaaatt aaggagtcac
     1981 tatggaaaaa ttcaatttca aaaacaatat cgggcaagaa aacaagttac ttcaaatcga
     2041 aatctacaag tttactaact tttgcaagct tcaaaattac acatctgtaa atattttttc
     2101 gaaagatata tttgaagcca tagt
//
LOCUS       DAVMEF010000207         2090 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVMEF010000207 DAVMEF010000000
VERSION     DAVMEF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.207"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..755
                     /locus_tag="VYQ30_001913"
     CDS             <1..755
                     /locus_tag="VYQ30_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1966965.1"
                     /translation="SYVYELYKDENYLKQPYYHNQGKKVLETLKKRFGLKDYISPEEV
                     ETDKNFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPE
                     NVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTVEGQAGMLDKELNIHAEEAKIRLRNL
                     NAAIENIQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLE
                     TLKKAFDLTNNEQKDQAVADDNEGDENDDADY"
     gene            742..2058
                     /locus_tag="VYQ30_001914"
     CDS             742..2058
                     /locus_tag="VYQ30_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1966966.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNPLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGSQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
BASE COUNT          700 a          339 c          405 g          646 t
ORIGIN      
        1 aatcttatgt ttatgaacta tataaagatg aaaactatct gaaacagccc tattatcata
       61 accaaggaaa aaaagtcctt gaaactttaa agaaaagatt tggtttgaaa gactatattt
      121 ctccagaaga ggttgaaaca gacaagaatt ttgtcttcat catcgatgag atcaatcgtg
      181 gggagatttc taagattttt ggcgaactct ttttctctat cgaccccggc tatcgtggtg
      241 aaaaaggaag tgtttctacc caatatgcaa atctacacga aactgatgaa aagttctata
      301 tccccgaaaa tgtttacatc atcggaacta tgaatgatat tgatcgttca gtggatacct
      361 ttgattttgc tatgcgtcgt cgttttcgtt ttgttgaagt tactgtcgag ggtcaagctg
      421 gcatgttgga taaagagttg aatatccatg cagaagaagc aaaaattcgt ctaagaaact
      481 tgaacgctgc tatcgaaaat attcaggaat taaacagtca ttatcatatt ggaccaagtt
      541 attttcttaa gttgaaggat gtagattttg actatgaatt actctggtct gattatatta
      601 agcctctcct agaagactac ttgcgaggtt cttatgatga ggttgaaact ttggaaactt
      661 tgaaaaaagc atttgatctg acaaataatg agcaaaaaga tcaggcagta gctgatgaca
      721 atgaaggcga tgaaaacgat gatgcggatt actgataatc aacacaagat tattaaagaa
      781 aaatttgttg aagaatatcc taaactaagc aatcctcttt tagacagaac cttggaaagt
      841 ctatcccaag atgaacgtat tttcattttt ccaaatgatt tgactcatac tcctgatttg
      901 gataaggacc aaaagatttt tgaaacagtc aatcagaaaa tcaagacagg gaacgtgatt
      961 ggttttcttg gatatggtca ggaaagatta acgatttcct cacgattttc tgatgagagt
     1021 aatgaccatt ttttgcatta tctcttaaac aaggttcttc atatcaatct cactagttta
     1081 gatgttgctt tgtctcgtga agagaggctt tatcaacttt tgatgtatct cttccccaag
     1141 tatctacaag ctgctattcg aaaaggtctt tataaggaat atcatcgatt ttctcataac
     1201 gacagtcatg ttaagggagt gattgatgta agaagccatc tcaagaaaaa tcttcctttc
     1261 acgggaaata ttgcctacac aacgagagag ttcacctatg ataatcccct catgcagttg
     1321 gtccgtcaca ctattgaata cattaagaat cagaaaagca ttggtcaagg ggtactagat
     1381 aatctctcaa ctagtcgtga aaacgtatct gaaatcgtgc gtgtaacgcc ctcttataaa
     1441 ctagctgatc gtgctaagat tattcgggga aatcaatcta aacctatacg tcatgcatac
     1501 tttcacgagt acagaaactt acaagaactt tgtctgatga tcctaaacca agaaaagcac
     1561 ggtttagggt atcaagatca aaaaatctat ggtattctct ttgatgttgc ctggctttgg
     1621 gaagagtatg tttacacctt gttgccaaaa ggttttgtac atcccagaaa taaggataag
     1681 acggatggaa tttcagtatt ttctgttggg aaacgaaaag tatatccaga tttttatgac
     1741 agagaacgaa agattgttct agatgcaaaa tataaaaaac tggaattgac tgaaaaagga
     1801 atcaaccgtg aggacttatt ccagctgatt tcctattctt atattttaaa agctgagaag
     1861 gctggactga tttttcctag tatggagcag tcagtaaata gtgaaatagg aaaagtagct
     1921 ggctatggtt ctcaattgaa gaagtggtct attcgaatcc ctcagaatgc ctcattttat
     1981 agtgcatttt gtaaaatgat ggaaaattca gaagaaaatt ttaaagcgat tattgatgaa
     2041 gaagtgggga gaaaataatt tcccctcttt ctttcatttt taacacaagt
//
LOCUS       DAVMEF010000208         2066 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVMEF010000208 DAVMEF010000000
VERSION     DAVMEF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.208"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(157..1414)
                     /gene="ltrA"
                     /locus_tag="VYQ30_001915"
                     /pseudo
     CDS             complement(157..1414)
                     /gene="ltrA"
                     /locus_tag="VYQ30_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          555 a          488 c          351 g          672 t
ORIGIN      
        1 actacatgat acaagacgaa acttaaaact agcttaactt ttctaaaatt ttactatttt
       61 gcaaaaaatt tctatcacca gcacctcacc aatcgagtag gggataatct ctagcccctc
      121 tcacaccacc gtacgtgccg tttggcatac ggcggttcaa ctaactttta acgcatgtcg
      181 ttcaaggtaa taatccaaac acgaaaccag tccacgtttt tccaggactg gttttgatat
      241 agcacgttta agtaccgact tctgagctac taattgataa tggtcgcccc agccagatac
      301 cttatctgct atccatttag gaactcctaa cttaagcaat ccccataatc gtctcgattt
      361 cttcttccat tgcttccaga taatcactcg taggcgagta cgcaagcgct catctatgct
      421 ggcgactata cttttcatat ttcccaatga gcaatagttt atccatcctc gaatagacaa
      481 attcagttgc tcaatacgtc ttgttaggtc tatactccat ttcctctgtg ttagtttctt
      541 caatttaaac ttaaatctcc gaacactatc ttgatgtgga cggcttttcc aaccatctga
      601 taatttccag aacccaaaac ctagatattt caactctctt ggtcatgttt actttcaaac
      661 ctagccgttt ctcaataaaa cgactgactg aatacatcac acgcttagag gctgcctcgc
      721 ttccgaccgt aatcacacaa tcatctgcgt agcgcacaaa tcgaagtccc ctcttttcta
      781 attccttgtc caattcatta agcatgatat tggataagag aggagataaa tttcctccct
      841 gtggtgtacc aactagtgtt ttataacgtt gaccattaat gataacacct gaatgaagat
      901 acttacgaat caaggattcc gtatctccgt cttcgataat gttatgtact aaggacatca
      961 atctatcttg aggaactgta tcgaaaaatt tctctaggtc tatatccact atccactcat
     1021 agccgtcatt taagtattct aagagcttca tgatggcttt ttcacatgac ctatttggtc
     1081 tgaaaccata actcgtatct gagaaatggg gttcacaaat ggggctcatg acttggacaa
     1141 tggcctgttg aatcattcta tccataactg ttggaattcc tagttgacgg atgcctccgt
     1201 ctggtttagg tatctcaact ctaagaactg gttgaggctt atattttctc tgttttatca
     1261 gttccttagt caagcgccag ttttgtctga gataattatc catctcttcg atagtcattc
     1321 catcaatccc agctgagcct ttattggatt ttacttgatt gtaggcttcc agcatatttt
     1381 cgcgtgataa tatcttatct agcagttttg acatgtgcgt attcctttct tgttttggtg
     1441 cctgagggac atcttatatt ggtgaacctt catctagtca ttacgtgtca aagtccagtt
     1501 ctatcagacc actcatttta cagacacaag tgaagtagga ttacttcgat tagttgttca
     1561 ccccttcgct ccacttccat tacggaagtt tcctcactac tatgggctcg gctgacttct
     1621 catgattcgt tgttactacg ctttcagcgc tcatgaaacc tcacgggata agtcgtacat
     1681 ctttcctcgt ttatacaaga tatgaggacg ttaaaaggtt acagtgaaaa taggaaatct
     1741 gacgcagagt ctttagactc tagaaagatt tatctttttc acacaaacct tagtccgaat
     1801 tcaattactc tcgtgattta cacatatagg ttacgactac ctttttggac tttagagttt
     1861 gaagcctcct tatccgctat atacgcctta ctatcacgtt cctgttcgta gagccacgat
     1921 ttcgctatcc cttcctctcc ctgctacctc acgatagtca ggctgaggac ttacacctca
     1981 gatatacgac atgcccgtcg taccgcaaaa aagccacctg attgggtggc ttcattagga
     2041 gattgtgatg aaaaaagttt aggatt
//
LOCUS       DAVMEF010000209         2040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVMEF010000209 DAVMEF010000000
VERSION     DAVMEF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.209"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..785
                     /locus_tag="VYQ30_001916"
     CDS             <1..785
                     /locus_tag="VYQ30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1966967.1"
                     /translation="SESYHKGESIEELALYAREKLGISKDNHDLLYKLERSGIYIVER
                     LINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMNEDGDRLE
                     QEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEYRAFKLGL
                     LTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSLATIMSKQ
                     SIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(837..1496)
                     /locus_tag="VYQ30_001917"
     CDS             complement(837..1496)
                     /locus_tag="VYQ30_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1966968.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSQVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            1566..1964
                     /locus_tag="VYQ30_001918"
     CDS             1566..1964
                     /locus_tag="VYQ30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1966969.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVALIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NRDIDSISLNVFEANQRAQNLYQKEGFEIV"
BASE COUNT          735 a          325 c          441 g          539 t
ORIGIN      
        1 tgagtgaatc ttatcataag ggagaatcca ttgaggaatt ggctctttat gccagggaaa
       61 aattaggtat ttcaaaagat aatcatgatt tgctttataa attagaacgt tcaggcatct
      121 atatcgtgga acgattaatt aatggccaag ctgatgctta tagcgcatgg tcaaaattgg
      181 gaagacctta tattgtgttg ggaacgaata aatcatctgt acgtcgaaat tttgacttag
      241 ctcatgagct aggacatatt cttttacata aatataaaga tatgaatgaa gatggcgatc
      301 gtttggagca agaagcaaat tattttgcat catgtttttt attgccaaaa gaagagtttt
      361 tagtcaaatt tgaagagagg gttggcaagc gtgtcagcaa tcctgatagt tatattttat
      421 tgaagtcgga tttgaatgtt tcgatacagg ctttagagta tcgagctttt aagttaggat
      481 tattgactcc aaagcaacat tcttactttt atcgtcaaat tgcgcaaaaa ggttacaaaa
      541 tgattgaacc tttggatgat caaatttttg ttaaaaaacc aagcaaagta aagagtattc
      601 tggacgttgt tttgagtaat catctagtca gtctagcgac tataatgtct aaacaaagta
      661 ttcgtttaca gtttataagc gaaatatttt cagtcgaaat gaaatttttt gatcagtatc
      721 aagaagatag aagaacagat cgatttgata acatcatccc tttgtacaaa agaaataatt
      781 tataatttaa ctgtgaaata atttcatgaa ggacatatgg cagaaattgg attttatcag
      841 acaagcagta gcttaccaaa aataatcaca caagcagcaa gtaagaggaa aggcacgaaa
      901 ggaagtcttt cctttttctt ttgcaggaga aaggccagga tacccgtcgc agaagcgaac
      961 tgaatcaaga tcagtaactc cgttacgcta aagacgagag cgcaagaagc taaaaaaagg
     1021 aaatcccctg cacccatgcg gatatcgata aaatgagcca aaattccaag tatgaggaag
     1081 gagaccatga ccagattcca gccagaggaa gctattagga ttaggtggaa agtcatccag
     1141 accagtaagg gatattcctg atggcgaaag tcgtagatgc ccaaggtcaa accagcagtg
     1201 attaggatga cttgactcaa ggaaagtaat tcccaagagt aaagcagaaa gaggagtcct
     1261 aagcctagtt caaagagggc ataccagaca ggatagcgga ccttgcagta gcgacagcaa
     1321 aagcgattga agacctgtga gagaatcgga atcaaatcta agggacgcaa gcgagtctga
     1381 caggaatcgc agtgactggc tgaactgata atggattgct ctggaaaacg gtcaatgacc
     1441 aaaccaagaa aggaagcgag aatgctcccg acgagaaaaa aataaaaatc aatcatactt
     1501 atctattcgt aaaaaatggg agaaatagta taatggagaa acatagataa gatggtgagg
     1561 aaaagatgac aattcgtttt gaagaaaagg tgagtataga aaacgctcaa tttgtatgcc
     1621 agtggtccaa ctccctaggc aaatcctttc aagaacaatg gatgggacca aggattcctt
     1681 ttctactgac ccttcaagcc ttggaaggag tcttttcaat ctttgatgaa caagagtttg
     1741 tggcacttat ccagaaaatc aggctagaag acagcaatct tcatatcggg agatttttta
     1801 tcaaccccca gaaacaggag caagacttag gtagccaggc tttaaggaaa tttgttagtt
     1861 tagcatttga aaatagagat atagacagta tttctctaaa tgtcttcgag gcaaatcaaa
     1921 gagctcagaa tctttaccaa aaagaaggat ttgaaatcgt ttaaatggtt gaagcacctg
     1981 tacgaaaata tagtagattg aaactagaat agtacacctc tacttctaaa acattgttag
//
LOCUS       DAVMEF010000210         2032 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVMEF010000210 DAVMEF010000000
VERSION     DAVMEF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.210"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            97..1986
                     /locus_tag="VYQ30_001919"
     CDS             97..1986
                     /locus_tag="VYQ30_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1966970.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSEFIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT          584 a          340 c          482 g          626 t
ORIGIN      
        1 attttatcga aaaatgcgta atttttcaac ataagtaagc ctttacttac gaaaaaatgc
       61 tataatgata agaaagataa aaagggggct tagttgatga aaaaaacttt tttcttactg
      121 gtgttaggct tgttttgcct tcttccactc tctgtttttg ccattgattt caagataaac
      181 tcttatcaag gggatttgta tattcatgca gataatacgg cagagtttag acagaagata
      241 gtttatcagt ttgaggagga ctttaaggga caaatcgtgg gacttggacg tgctggtaag
      301 atgcctagcg ggtttgacat tgaccctcat ccaaaggttc aggccgcgaa aaacggtgca
      361 gaactagcag atgtgactag cgaagtaaca gaagaagcgg atggttatac tgtgagagtc
      421 tataatccag gtcaggaggg cgacatagtt gaagttgacc tcgtctggaa cttaaaaaat
      481 ttacttttcc tttatgatga tatcgctgaa ttaaattggc aacctctgac agatagttca
      541 gagtttattg aaaagtttga atttcatgta aggggagaca agggggctga aaaactcttt
      601 ttccatacag ggaaactttt tagagaggga acgattgaaa agagtaacct tgattatact
      661 atccgtttag acaatcttcc ggctaagcgt ggagttgagt tgcatgccta ttggcctcgg
      721 accgattttg ctagcgctag ggatcaggga ttgaaaggga atcgtttaga agagtttaat
      781 aagatagaag actcgattgt tagagaaaaa gatcagagta aacaactcgt tacttgggtc
      841 ctcccttcga tactttctat ctccttgtta ttgagtgtct gcttctattt tatttataga
      901 agaaagacca ctccttcagt caaatatgcc aaaaatcatc gtctctatga accaccaatg
      961 gaattagagc ctatggtttt atcagaagca gtctactcga cctccttgga ggaagtgagt
     1021 cccttggtca agggagctgg aaaattcacc tttgatcaac ttattcaagc taccttgcta
     1081 gatgtgatag accgtgggaa tgtctctatc atttcagaag gagatgcagt tggtttgagg
     1141 ctagtaaaag aagatggttt gtcaagcttt gagagagact gcctaaatct agctttttca
     1201 ggtaaaaaag aagaaactct ttccaatttg tttgcggatt acaaggtatc tgatagtctt
     1261 tatcgtagag ccaaagtttc tgatgaaaaa cggattcaag caagagggct tcaactcaaa
     1321 tcttcttttg aagaggtatt gaaccagatg caagaaggag tgagaaaacg agtttccttc
     1381 tgggggctcc cagattatta tcgtccttta actggtgggg aaaaggcctt gcaagtgggt
     1441 atgggtgcct tgactatcct gcccctattt atcggatttg gtttgttctt gtacagttta
     1501 gacgttcatg gctatcttta cctccctttg ccaatacttg gttttctagg gttagttttg
     1561 tctgttttct attattggaa gcttcgacta gataatcgtg atggtgttct aaatgaagcg
     1621 ggagctgagg tctactatct ctggaccagt tttgaaaata tgttacgtga gattgcacga
     1681 ctggataagg ctgaattgga aagtattgtt gtttggaatc gtctcttggt ctatgcaacc
     1741 ttatttggct atgcggacaa ggttagtcat ttgatgaagg ttcatcagat tcaagttgaa
     1801 aatccagata tcaatctcta tgtagcttat ggctggcaca gtatgtttta tcattcaagc
     1861 gcgcaaatga gccattatgc tagtgtcgca aatacagcaa gtacctactc cgtatcttct
     1921 ggaagtggaa gttctggcgg tggcttctct ggaggcggag gtggcggcag tatcggagcc
     1981 ttttaaagag agctaccata gactgaaaaa gtatgatata atggaagata ga
//
LOCUS       DAVMEF010000211         2008 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVMEF010000211 DAVMEF010000000
VERSION     DAVMEF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.211"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..1851
                     /gene="pspA"
                     /locus_tag="VYQ30_001920"
     CDS             4..1851
                     /gene="pspA"
                     /locus_tag="VYQ30_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV1966971.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPAPKPAPAPAPTPEAPAPAPKPAPAPKPAPAPKPAPAPKPEKPAEKPAPAPK
                     PETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWAKVNGLWYYL
                     NSNGAMATGWLQYNDSWYYLNTNGAMATGWLEYNGSWYYLNANGAMATGWLEYNGSWY
                     YLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDT
                     WYYLEASGAMKANQWFKVSDKWYYVNGLGALAVNTTVDGYTVNENGEWV"
BASE COUNT          743 a          379 c          441 g          445 t
ORIGIN      
        1 tagatgaata agaaaaaaat gattttaaca agtctagcca gcgtcgctat cttaggggct
       61 ggttttgttg cgtctcagcc tactgttgta agagcagaag aagctcccgt agctaagcag
      121 tctcaagctg agagagacta tgatgcagca atgaaaaaat ctgaagctgc taagaaagaa
      181 tacgaagaag ctaagaaaga tttagaagaa gcaaaagctg ctcagaaaaa atatgaagac
      241 gatcaaaaga aaactgaaga gaaagcaaag cttgttcaaa aagcggatga agagcgtcaa
      301 aaagcgaatg tggcagttca aaaggcgtat ttaaaactcc gagaagcaca agaacaattg
      361 aacaaaagtc caaacaataa gaaaaatgcc gctcaacaga aattaaaaga tgcattagct
      421 catattgatg aggtaacatt aaaacaaaaa gaggcggaag cgaatttcaa taaggaacaa
      481 gcaaaagtag ttcctgaagc agataagtta gctgagacta agaaaaaagc agaacaggct
      541 gaaaaaaaag aaccagaact tgctaaaaaa gtagcagaag ctaaagcaaa agcagaagaa
      601 gctgagaaaa aagctgttga agccaaacaa aaagtggatg ctgaaaaata tgctcttgaa
      661 gctaaaatcg ctgagttgga atatgaagtt cagggactag aaaaagagct caaagagatt
      721 gatgagtctg actcagaaga ttatattaaa gaaggtctcc gtgctcctct tcaatctaaa
      781 ttggatgcca aaaaagctaa actatcaaaa cttgaagagt tgagtgataa gattgatgag
      841 ttagacgctg aaattgcaaa acttgaaaaa gatgtagaag atttcaaaaa ctcagacggt
      901 gagcaagctg aacaatatct tgttgcagct aaaaaagact tagatgctaa aaaagctgaa
      961 ttagaaaata ctgaagctga ccttaagaaa gcagttgatg agccagaaac tccagctccg
     1021 gctccggctc ctaaaccagc cccagctcca gctccaactc cagaagcccc agctccagct
     1081 cctaaaccag ctccggctcc taaaccagct ccggctccta aaccagctcc agctcctaaa
     1141 ccagaaaagc cagcagaaaa accagctcca gctcctaaac cagaaactcc aaaaacaggc
     1201 tggaaacaag aaaatggtat gtggtacttc tacaatacgg atggttcaat ggcaacaggc
     1261 tggctacaaa ataatggctc atggtactac ctcaacgcta atggtgctat ggcaacaggt
     1321 tgggctaaag tcaacggctt atggtattac ctcaacagca atggcgctat ggcgacagga
     1381 tggctccaat acaatgattc atggtactac ctcaatacta atggtgctat ggcgacagga
     1441 tggctagaat acaatggttc atggtactac ctcaacgcta atggtgctat ggcgacaggt
     1501 tggctagaat acaatggctc atggtactac ctcaacagca atggcgctat ggcgacaggt
     1561 tggctccaat acaatggttc atggtactat ctaaacgcta acggcgctat ggcaacaggt
     1621 tgggctaaag tcaacggttc atggtactac ctcaacgcta acggttcaat ggcaacaggt
     1681 tgggtgaaag atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaaac
     1741 caatggttca aagtatcaga taaatggtac tatgtcaatg gcttaggtgc ccttgcagtc
     1801 aacacaactg tagacggata tactgtcaat gaaaacggtg aatgggttta agccgattaa
     1861 attaaatcat gttaagaaca tttgacattt taattttgaa acaaagataa ggttcgattg
     1921 aatagattta tgttcgtatt ctttaggtac ctgtcttatg atttcaggaa atgtcattaa
     1981 aaaaacgact ccttttctct aacttgga
//
LOCUS       DAVMEF010000212         1997 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVMEF010000212 DAVMEF010000000
VERSION     DAVMEF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.212"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            405..1880
                     /locus_tag="VYQ30_001921"
     CDS             405..1880
                     /locus_tag="VYQ30_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1966972.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVVELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          589 a          372 c          459 g          577 t
ORIGIN      
        1 atatagtaat aaagcttaga aaatgagatg atgttttcta gcaaatataa acccgagtaa
       61 aaaatgccta cggacaggca gggttgaatg ccgaagcgtg gttgaaaagc cacattattg
      121 atagggttaa aagcctactt ttataagttg atgttaggac acttgttcta attcataaat
      181 ttttagtgtg gtgaaagcac acgtcatctt gtgaaacgat caataaagta cgtaatattt
      241 gctactagag agttaggaaa catcgggaac agacatactc aacagaaacc aaaataaaca
      301 cgtcagaaga ttgcagagca ggtgaaaacc tgctcttttt tcatgagtca acctttagtt
      361 ccttagtttt cataaggtcc taaaaatatt gaaaggagta tgttttgaaa gagttagatc
      421 aaaaccaagc cccaatttat gaggccttgg tgaagttacg caagaaaagg attgttccct
      481 ttgatgttcc aggtcacaag cgtggacggg gaaatccaga acttgtcgaa ctcttaggag
      541 aaaaatgtgt aggcattgat gtcaattcga tgaaaccctt ggataattta ggccatccta
      601 tttcgattat tcgtgatgca gaggagctgg ctgcagatgc ttttggagct agccatgcct
      661 ttctaatgat tggtggaaca acttcatcgg tgcagactat gattctggca acctgcaagg
      721 caggagataa gattattctg ccacgaaatg tccataaatc tgctatcaat gcgttggttc
      781 tatgtggtgc cattcccatc tatatcgaga tgagtgtaga tcctaagatt ggtatcgctt
      841 taggtcttga aaatgaccga gtagcacagg ccataaagga ccatccagat gctaaggcta
      901 tcctaatcaa caatcctact tactacggca tctgttcaga cctaaagggg ttgacagaaa
      961 tggctcatga agctggcatg atggttttag tagatgaagc ccatggagcg catttgcatt
     1021 tcactggtaa acttccaatt tctgctatgg atgcaggagc tgatatggca gcagtttcca
     1081 tgcataagtc tggtgggagt ttgacacaaa gttcgcttct tttaatcggg gagcagatga
     1141 atcccgagta cgttcgtcag attataaacc tgacccagtc tacatctgcc tcttacttat
     1201 tgatggctag tttggatatt tcacgtcgca acttggccct tcgtggtaaa gagtcgtttg
     1261 aggaagtcgt tgagctatcc gagtacgctc gtcatgaaat caatgccatt ggcggttact
     1321 atgcttactc aaaagagtta atagatggtg tgtcggtctg tgattttgat gtaaccaagt
     1381 tgtcagttta cactcaggga ataggcttaa caggtatcga ggtttatgac ctcttgcgag
     1441 acgaatacga cattcagatc gagtttggtg atatcggcaa tatcttggcc tatatttcca
     1501 tcggcgaccg catccaagac atcgagcgct tggttggtgc tctggctgat attaagagac
     1561 tctattcacg agatgggaag gacttaatag ctggagaata tattcagccc gagttggtgc
     1621 tgtctccaca agaagctttc tattcagaga gaaaaagttt gaccttagat gagtctgttg
     1681 gacaggtctg tggagaattt gtcatgtgct atcctccagg gattcctatc ttggctcctg
     1741 gtgaacgcat tacacgagaa attgtagact atatccaatt cgccaaggaa cgtggttgct
     1801 ccctccaagg gacggaagat ccagaggtca atcatatcaa cgttattaag agaaagacaa
     1861 actataagaa aagtcaatag ttttatctaa actatttctt atttcaattt gatgatttgg
     1921 cgatgatttt agagcacggc aaaaagccct tgaattagaa gcggtcaatc gcttaatttc
     1981 tatcagctta tcaaatc
//
LOCUS       DAVMEF010000213         1995 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVMEF010000213 DAVMEF010000000
VERSION     DAVMEF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.213"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            142..723
                     /locus_tag="VYQ30_001922"
     CDS             142..723
                     /locus_tag="VYQ30_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1966973.1"
                     /translation="MYYAENPDAAHDIYELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            720..>1995
                     /locus_tag="VYQ30_001923"
     CDS             720..>1995
                     /locus_tag="VYQ30_001923"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1966974.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
BASE COUNT          588 a          319 c          503 g          585 t
ORIGIN      
        1 ggtttattat ttataagtgt tgaaagagat tgaagaaaat ggcttcgtga ctcgctttct
       61 tcggtctttt tcgttttatt atcaatttgt aaacgattta caatttgagt caaattatgg
      121 taaaatagtg tcatgagtaa aatgtattat gcagaaaatc ctgacgctgc tcacgacatt
      181 tatgagttga gagtagagtt gttgggacaa aaaatgacct ttttaacgga tgcgggtgtt
      241 tttagcaaga aaatagttga ctttggaagt caactattgc tcaagtgtct tgaggtcaac
      301 caaggagaaa ctgtccttga tgtaggctgt ggttacggac ctttgggttt gtccttagct
      361 aaggcttatg gagttcaggc tactatggtt gatatcaaca ctcgtgcctt agatttagct
      421 cggagaaatg ctgaaaaaaa taatgcaaaa gcgacgattt tccagtccaa catctatgaa
      481 caagttgaag gacattttga tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa
      541 gttgttcatg aaatcattga gaaaagtaaa gattttttgg aaactggtgg ggatttgaca
      601 atcgtcatcc agaaaaaaca aggggctcca agtgctaagt ccaagatgga ggatgtgttt
      661 ggtaattgtg aaatccttaa aaaagacaaa ggatactata tccttagaag tgtgaaagaa
      721 tgagagcagt tgatttaatc caaaaaaaac gagacggtca agaactgact tcaagtgaaa
      781 ttgaatggct gatagaaggc tatgtatcag gaactgttcc tgattatcag atgtctgcct
      841 ttgctatggc tgtttatttt aaaggaatga cgactcgaga aatctctgat ttaacgatga
      901 atatggttaa gactgggcaa gagtttgacc tgtcagccat tgatggtgtg aaagttgaca
      961 agcattctac tggtggtgtc ggcgataagg taaccttgat tttagctcct cttgttgcta
     1021 gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct tggccataca ggcggaacaa
     1081 ttgataaatt ggagtccatt aagggctatc aagtagaacg tagtcaagag gattttattc
     1141 gtcaggttca ggatattgga gtatctgtca ttgggcaatc agaccagctg gtcaaagcag
     1201 ataagcttct ctatgccctt cgtgatgtga ccgcaactgt cgacacgatt cctttgattg
     1261 cgagttcggt gatgagcaag aaaattgcgg caggggcgga tgctattttg ctagacgtga
     1321 ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta
     1381 tggtagatct tggtaaggtt gttggacgga agacagtagc agtgattacg gatatgagcc
     1441 agcctttggg acgagcgatt ggaaatcgtc tggaaatcct tgaagcattg gagattttgc
     1501 aaggtcaagg acgtcaggat attacccact ttatttgtga attggctcaa attatgcttg
     1561 gtttggcaaa tgttaacaaa actgttgaag aagttcgcca acatcttgag aatggtcaag
     1621 cactggctaa gtttgaggaa atggtgcaag cccaaggtgg agacttggaa gacctctatc
     1681 gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca ggaaacgggt gtcatttcag
     1741 ctcttccagc tatggacttt ggtctttatg ccatgaggct gggtgctggt cgtgcagtta
     1801 agtctgatgc cttggactat gaaacaggaa ttgtttttga aaagaagatt ggagactccg
     1861 ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga aaaaattcca cctcaactag
     1921 ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg
     1981 tagaaatcat ctcct
//
LOCUS       DAVMEF010000214         1982 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVMEF010000214 DAVMEF010000000
VERSION     DAVMEF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.214"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            5..1237
                     /gene="ilvA"
                     /locus_tag="VYQ30_001924"
     CDS             5..1237
                     /gene="ilvA"
                     /locus_tag="VYQ30_001924"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1966975.1"
                     /translation="MIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENAQRVRSF
                     KIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITTPQQKIG
                     QVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGTVAYEIL
                     EEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKAAFEAGG
                     PVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSKQGIVAE
                     PAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHYFVVNFP
                     QRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLIRRMEGF
                     DPAYINLNGNETLYNMLV"
BASE COUNT          589 a          321 c          434 g          638 t
ORIGIN      
        1 agatataatc aaggctcaca aggtcttgaa cggtgtggtt gtgaatactc cactggatta
       61 cgatcattat ttatcggaga agtatggtgc taagatttat ttgaaaaaag aaaatgccca
      121 gcgtgttcgc tcctttaaaa ttcgtggtgc ctattatgcc atttcccagc tcagcaagga
      181 agaacgtgaa cgtggggtag tctgcgcttc tgcgggaaat catgcgcagg gagtagccta
      241 tacttgtaat gaaatgaaaa ttcctgctac tatctttatg cctattacta cgccacaaca
      301 aaagattggt caggttcgct tttttggtgg ggattttgta actattaaac tagttggaga
      361 tacctttgat gcctcagcca aagcagctca agaatttaca gtctctgaaa atcgtacctt
      421 tattgatcct tttgatgatg ctcatgttca agcaggtcaa ggaacagttg cttatgagat
      481 tttagaagaa gctcgaaaag aatcgattga ttttgatgct gtcttggttc ctgttggtgg
      541 tggcggtctc attgccgggg tttctaccta tatcaaggaa acaagtccag agattgaggt
      601 tatcggagta gaggcgaatg gagcgcgttc catgaaagct gcctttgagg ctggaggtcc
      661 agtcaaactc aaggaaattg ataaatttgc ggatgggatt gctgtgcaaa aggtaggtca
      721 gttgacctat gaagcaactc gtcaacatat taaaactttg gtaggtgtcg atgagggatt
      781 gatttctgaa accttgattg acctttactc taagcaaggg atagtcgcag aacctgctgg
      841 agcggctagt atcgcctctt tagaggtttt agctgaatat attaagggga aaaccatttg
      901 ttgtatcatt tctggaggaa ataatgatat caaccgtatg ccagaaatgg aagagcgtgc
      961 cttgatttat gatggtatca aacattactt tgtggtcaat ttcccacaac gtccaggagc
     1021 tttgcgtgag tttgtaaatg atatcctggg gccaaatgat gatatcacac gttttgagta
     1081 tatcaaacga gctagcaagg gaacaggccc agtattaatt gggatcgctt tagcagataa
     1141 gcatgattat gcaggtttga ttcgtagaat ggaaggtttt gatccagctt atattaactt
     1201 aaatggtaat gaaacgcttt ataatatgct tgtctgagga ctaataaaaa aatatcatat
     1261 tatttgtaca actgatgtat atgtgctata atgaagatga agaaagggga cgattcctat
     1321 ggttttagaa aacttatcgt gcaattctta cttctgttca ttatagcact tatgatgttt
     1381 ggctcagatt tccatccttc attttgattt cctatctatt gacaagcata gtcacactgt
     1441 ctttaatact cttcgaaaat ctcttcaaac cacgttagct ctatctgcaa cctcaaaaca
     1501 gtgttttgag caacttgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa
     1561 ggtatgattt gatttctttt tgttgacaaa tataccatat taaaaagata tataagtaat
     1621 taactgagct tatctgtctt gtcatcttta ttaaggatgg tttagataat cgggtgtctg
     1681 cttctaggct agcacctcaa tatccaaagg agtgatgaat ttgaaggaca taaggaatac
     1741 ctatctctca gatgatttat tgaggaagaa agataggagt ttttgagcta gtgaaggctt
     1801 ggatttctaa aggttagaac tatcattttc agttcttaaa tcgaagaaat aagctatctt
     1861 acggaaatag agaagcattt tttaagaact tgaataattt cgcaccttaa gagggtaata
     1921 atacagtatt tttattagca aatatttatg gtgtagaggc tagcaaaacc tatatattat
     1981 cg
//
LOCUS       DAVMEF010000215         1951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVMEF010000215 DAVMEF010000000
VERSION     DAVMEF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.215"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            405..566
                     /locus_tag="VYQ30_001925"
     CDS             405..566
                     /locus_tag="VYQ30_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1966976.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            658..>1951
                     /locus_tag="VYQ30_001926"
     CDS             658..>1951
                     /locus_tag="VYQ30_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1966977.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLD"
BASE COUNT          759 a          240 c          412 g          540 t
ORIGIN      
        1 tttgttacat caagataaat attttctgat ggtcaaattt taaaaagatt aataactctc
       61 gataaatttt gaaccttaac aattgaatag accaagacaa gacgttaatt atttttggag
      121 cgtaataacc tatccgtcaa gatctttcgg ctgaataatt cggcaaaaaa agttctgcta
      181 agcaaaaaga aagtgagcga taagtaagag tttgaatata gggagggata ccctatccgc
      241 tatgaagaaa atgaggataa tgatacttct aaggttgaag ccggctcatc cggaatagag
      301 gcggaggtga gattcctatg ttgctaatcc aaagcacttg tcaatgtcaa aaaacttaat
      361 taaacaaata attattagat ataaaacttt agaggaggta tatattggac aatgactgga
      421 atggattggc tgatttaata gcaaatctga ttgcaaaata tgctggtgct ttagatttag
      481 ataatctacc cgatccaacg ccagcaaaaa atcaagagat gaaaaatagt tttgacatgg
      541 caaaaacaca aattgagacg gactaataaa agtgttataa tatacttgaa atgatagtcc
      601 aaacttaaat taaaaaaatt atatatgata tagaaaaatt atattgaggt taatactatg
      661 tcaaaagaaa aaataaaagt atacctctat acacgagtat ctacgtcaat acagatagag
      721 gggtattcgt tagaggcaca aaaatcacga atgaaagctt ttgctattta caacgattat
      781 gaaattgttg gagagtatga agatgccggg aagtctggca agtctattga aggtagaata
      841 cagttcaatc gaatgatgga agatataaaa tctggaaaag atggagtatc ttttgttctt
      901 gtgtttaagt tatcaagatt tgcaagaaat gccgctgatg ttctttctac acttcagata
      961 atgcaggatt atggagttaa tcttatctgc gttgaagatg gcattgattc atctaaagat
     1021 gctgggaaac taatgatttc tgttttatca gctgtggctg aaatagaaag agaaaacatc
     1081 cgtattcaaa caatggaagg ttgcattcaa aaagcaaggg aaggaaaatg gaatggtggc
     1141 tttgctccgt atggatataa acttgaagac ggcaagctgt ttataaatga ggaagaggca
     1201 gttgcaataa gaacgatttt cgaccagtat gtaaatacta cgataggagc caatgggata
     1261 tctaaatact tagagaatca tggaattaga aaaatcccaa gacagaatgg taagaatcct
     1321 ttgtttgatg caggtcttat aagaaagata ttaaagaatc ctgtatataa tgggaaaata
     1381 gcatttggaa gaagaacttt agaaaaagtt catggtacaa gaaatgaata taagcaggtt
     1441 gaacaagatg aatatctaat atctgaaggg atacatgaag ctatagtttc cgatgaagtt
     1501 tggcaagctg ctcaggttaa gctaaaatct caagcaaaga aatatgagca tgtgaataaa
     1561 ggaaaagata cacgcacaca cttgctttct ggaattgtaa aatgcccgat atgtggagtg
     1621 ggaatgtttg ggaacaagtg tatcaagaaa aagaaagatg gcacaaagta taaagatttt
     1681 tattactatg gttgtaaaca taggcagatg ataagaggtc ataagtgtac attcagtaag
     1741 caaattagag aagaattgtt agatgatgct gttgcagagg tgattgtcaa gatagtaagt
     1801 aatccgaaat ttgcttctat gatgcaagaa aaaatcaaca tgaaggtgga tacctctgaa
     1861 atagaaaaag aaatagataa ttaccaaaag gaattgagaa agagtcattc tacaaagttt
     1921 aagctaattg aggaaataga taatttagat g
//
LOCUS       DAVMEF010000216         1917 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVMEF010000216 DAVMEF010000000
VERSION     DAVMEF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.216"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..977
                     /locus_tag="VYQ30_001927"
     CDS             <1..977
                     /locus_tag="VYQ30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1966978.1"
                     /translation="SACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATI
                     AWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFE
                     AISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEK
                     EGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYL
                     TDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLGK
                     INAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            967..>1917
                     /locus_tag="VYQ30_001928"
     CDS             967..>1917
                     /locus_tag="VYQ30_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1966979.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAI"
BASE COUNT          673 a          277 c          343 g          624 t
ORIGIN      
        1 ttagtgcttg ttcttctaat tctgttaaaa atgaagaaaa tacttctaaa gagcatgcgc
       61 ctgataaaat agttttagat catgctttcg gtcaaactat attagataaa aaacctgaaa
      121 gagttgcaac tattgcttgg ggaaatcatg atgtagcatt agctttagga atagttcctg
      181 ttggattttc aaaagcaaat tacggtgtaa gtgctgataa aggagtttta ccatggacag
      241 aagaaaaaat caaagaacta aatggtaaag ctaacctatt tgacgatttg gatggactta
      301 actttgaagc aatatcaaat tctaaaccag atgttatctt agcaggttat tctggtataa
      361 ctaaagaaga ttatgacact ctatcaaaaa ttgctcctgt agcagcatac aaatctaaac
      421 cttggcaaac tttatggaga gatatgatta aaattgattc aaaagcctta ggtatggaaa
      481 aagaaggtga tgagttaatc aaaaatactg aagctcgtat atccaaagaa ttagaaaaac
      541 atccagaaat caaaggaaaa atcaaaggaa aaaaagtatt atttactatg attaatgctg
      601 cagatacatc aaaattctgg atttatacta gcaaagatcc aagagcaaat tatttaacag
      661 atttaggtct agttttccct gaatcattaa aagaatttga gagtgaagat agttttgcaa
      721 aggaaatttc tgcagaagaa gcaaataaga taaatgatgc tgatgtaatc ataacttatg
      781 gtgatgataa aactcttgaa gctttacaaa aagatcctct tttaggtaaa ataaatgcaa
      841 ttaaaaatgg tgccgttgct gtaattccag ataatacacc gttagcagcc tcatgcactc
      901 caacaccact ttcaataaac tatactattg aagaatatct aaatctttta ggaaatgcat
      961 gcaaaaatgc gaaataaaaa acaaataaac ctaggcataa tttttgtaat ctgcctaggt
     1021 cttcttatta caatattttt gtcattaaag cttggaacaa aagaaattaa tatcagagat
     1081 tttttagcag cttttggaat gggtaataca aatgatgatt ttattaaatc aattatatat
     1141 aaaagaatac ctagaactat ttttgcaatt ttagcaggtt ctagtcttgc cataagcggt
     1201 gtattgatgc aatcagttac tagaaaccca atagctgatc caggtatact cggtataaac
     1261 acaggagcaa gtcttagtgt agtaattggt ctttcttttt taggaatttc atcaagcata
     1321 agccatataa gttttgcaat cattggtggc ttagtaagtg caatttttgt atacgcgatt
     1381 gctgtaagcg gaaaagcagg ccttacccct ataaaacttg ccttatcagg aacttgtgtt
     1441 agtatggctt taagcagttt tgtaagtttt ttaattttac cgaataataa cgtcttagac
     1501 aaatttagat tttggcaaat aggtagcctt ggagcagcta cattatcttc tatatctaca
     1561 ctactacctt ttataatttt aggtcacttg atagctatat ttatttcatc agatttaaac
     1621 gctttagcta tgggtgatga aatggctgtt ggtcttggag ttaatgttaa taggataaga
     1681 tcacttgcaa taattgcaag tgtgctttta tgttcaagta ttactgcaat tggtggacct
     1741 attggcttcg taggtcttat agttcctcac ttttgtggct tatttataag caaagatata
     1801 cgcacaatga ccatttcttc atcttttata ggtgcagagc tcttgcttat atgtgatata
     1861 atcggccgta tgttaggtaa accaggtgaa attgaagtag ggataattac tgcaata
//
LOCUS       DAVMEF010000217         1890 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVMEF010000217 DAVMEF010000000
VERSION     DAVMEF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.217"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            5..1186
                     /locus_tag="VYQ30_001929"
     CDS             5..1186
                     /locus_tag="VYQ30_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1966980.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1244..>1890
                     /locus_tag="VYQ30_001930"
     CDS             1244..>1890
                     /locus_tag="VYQ30_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1966981.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLR"
BASE COUNT          688 a          337 c          350 g          515 t
ORIGIN      
        1 aaatatgaaa catctaaaaa cattttacaa aaaatggttt caattattag tcgttatcgt
       61 cattagcttt tttagtggag ccttgggtag tttttcaata actcaactaa ctcaaaaaag
      121 tagtgtaaac aactctaaca ataatagtac tattacacaa actgcctata agaacgaaaa
      181 ttcaacaaca caggctgtta acaaagtaaa agatgctgtt gtttctgtta ttacttattc
      241 ggcaaacaga caaaatagcg tatttggcaa tgatgatact gacacagatt ctcagcgaat
      301 ctctagtgaa ggatctggag ttatttataa aaagaatgat aaagaagctt acatcgtcac
      361 caacaatcac gttataaatg gcgccagcaa agtagatatt cgattgtcag atgggactaa
      421 agtacctgga gaaattgtcg gagctgacac tttctctgat attgctgtcg taaaaatctc
      481 ttcagaaaaa gtgacaacag tagctgagtt tggtgattct agtaagttaa ctgtaggaga
      541 aactgctatt gccatcggta gcccgttagg ttctgaatat gcaaatactg tcactcaagg
      601 tatcgtatcc agtctcaata gaaatgtatc cttaaaatcg gaagatggac aagctatttc
      661 tacaaaagcc atccaaactg atactgctat taacccaggt aactctggcg gcccactgat
      721 caatattcaa gggcaggtta tcggaattac ctcaagtaaa attgctacaa atggaggaac
      781 atctgtagaa ggtcttggtt tcgcaattcc tgcaaatgat gctatcaata ttattgaaca
      841 gttagaaaaa aacggaaaag tgacgcgtcc agctttggga atccagatgg ttaatttatc
      901 taatgtgagt acaagcgaca tcagaagact caatattcca agtaatgtta catctggtgt
      961 agttgttcgt tcggtacaaa gtaatatgcc tgccaatggt caccttgaaa aatacgatgt
     1021 aattacaaaa gtagatgaca aagagattgc ttcatcaaca gacttacaaa gtgctcttta
     1081 caaccattct atcggagaca ccattaagat aacctactat cgtaacggga aagaagaaac
     1141 tacctctatc aaacttaaca agagttcagg tgatttagaa tcttaattga catctatgta
     1201 aagaaagctt tacataagag aaaagatgtg ttagtgtaga atcatggaaa aatttgaaat
     1261 gatttctatc acagatatac aaaaaaatcc ctatcaaccc cgaaaagaat ttgatagaga
     1321 aaagctagat gaactagcac agtctatcaa agaaaatggg gtcattcaac cgattattgt
     1381 tcgtcaatct cctgttattg gttatgaaat ccttgcagga gagagacgct atcgggcttc
     1441 acttttagct ggtctacggt ctatcccagc tgttgttaaa cagatttcag accaagagat
     1501 gatggtccag tccattattg aaaatttaca gagagaaaat ttaaacccaa tagaagaagc
     1561 acgcgcctat gaatctctcg tagagaaagg attcacccat gctgaaattg cagataagat
     1621 gggcaagtct cgtccatata tcagcaactc cattcgttta ctttccttgc cagaacagat
     1681 tctctcagaa gtagaaaatg gcaaactatc acaagcccat gcgcgttccc tagttgggtt
     1741 aaataaggaa caacaagact atttctttca acggattata gaagaagata tttctgtaag
     1801 gaagttagaa actcttctga cagagaaaaa acaaaagaaa cagcaaaaaa ctaatcattt
     1861 catacaaaat gaagaaaaac agttaagaaa
//
LOCUS       DAVMEF010000218         1857 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVMEF010000218 DAVMEF010000000
VERSION     DAVMEF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.219"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..613)
                     /locus_tag="VYQ30_001931"
     CDS             complement(<1..613)
                     /locus_tag="VYQ30_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966982.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMD"
     gene            complement(601..1158)
                     /locus_tag="VYQ30_001932"
     CDS             complement(601..1158)
                     /locus_tag="VYQ30_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1966983.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGISFKNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            complement(1059..1688)
                     /locus_tag="VYQ30_001933"
     CDS             complement(1059..1688)
                     /locus_tag="VYQ30_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1966984.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            complement(1699..>1857)
                     /locus_tag="VYQ30_001934"
     CDS             complement(1699..>1857)
                     /locus_tag="VYQ30_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966985.1"
                     /translation="SHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKT
                     KMAEEFIY"
BASE COUNT          609 a          358 c          233 g          657 t
ORIGIN      
        1 tgtccatcaa ataataaatt ctatgctctg caacaattat gcttatgcct ttctctttta
       61 atatctttag catctttgcc aaaacactta tgcttttaat atctaaattc gatgaaggct
      121 catccataac tataatcttt gtacctgcta tataagaagc tgcaatgcaa agaatttgtt
      181 tttcaccgcc ggatagatta aatatatttc tgttcaaaag attttttatc gggaatatct
      241 taagcatatc cttcaaacgc ctgtccatct cttctcttgc aagaccgata ttttccaaat
      301 aaaataataa ttctaatgtc gtattgacat taaaaaaata tgtcttagga ttttgaaaaa
      361 cagttgaaac aagcattgag atttgataaa gttccaaatt ttttatattc ttaccatcaa
      421 taattatttc gccctttgtc tttgcgttat cataccttac agctagtcca ttgattgaat
      481 ttattaaaga tgacttacca tttccacttt ttcctgtaaa tagcacgcac tctccctcgg
      541 ctatggatag tgatatatca tctaatatat gctcttcacc ataatcaaga cttaaatttt
      601 ttatttcaac catattaagc ctcccacaat aagtccagca accgctaaaa cataaacaaa
      661 atcaattagc tgtatcttta cgcgaatgta tctggtcttg gcaattggat tttctattgc
      721 ctttgtttct gccgcttttg caatgtcatc tgatatatta gatgatataa tgagtagtgg
      781 catagaaaca tattcaagat atttgactgg gtttttaaaa cttatccctc ttactctcat
      841 agccattttg atgtttttct tctcctcctt aaaagatggg aagaatctaa acataaccgc
      901 aataggtatg gatacattct ttgaaatctt aagcttatcc atcgatgaaa ttattgcgcc
      961 tacatccgag gtctttatca taaagcttgc tgccataaat ggtaaaataa acattctaat
     1021 aacaataggt atactcaaaa acatcttaac tatagggttt aattcagata acaccataaa
     1081 attaggcaag gagtaaagta tcccacacaa tataatccat ttgaaaagtg ttttttcaat
     1141 agagccttgc ctatatatgc tccaagaatt cctcctaaga aagcacctaa ggctactaaa
     1201 gccatgtgag gataagttat taacttttct aatacatcaa cataatcttt tcccatcgtc
     1261 atcaaagacc actccatata tttttctttt gctaaaagca tttgcattaa agagctacat
     1321 atccatgtgc tgaagattgc ataagaaagc atattgtatt taaatgaatt ataattacca
     1381 atctttctaa ttaattctgc tattatcatt actataagtg ataaaaccac aactacgtag
     1441 gtatgaccag ctgcaaacat cacaagtggt gatatcatac caaatataaa tagtgcccat
     1501 ggcttttgaa ccttagccat aaataacata acaacagttc ctgcaactat ggctaatact
     1561 gtcgggtata ttaagaacaa tattggaatc aagcccatca taccaactcc aaacatgaag
     1621 gcaaaataaa ttacgccaaa aacaccgata cttactaaat cttttacttt taatttttta
     1681 ttatccattg tagtcctcct aataaataaa ttcttctgcc atttttgtct tttctattaa
     1741 atttttgtaa atttttgatt tttgtaaaag ctcttcatgc ttaccttcgc tttctactct
     1801 tccgttttga agaactacta tcttgtctgc attttctatg gatttcattc tatgtga
//
LOCUS       DAVMEF010000219         1836 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVMEF010000219 DAVMEF010000000
VERSION     DAVMEF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.220"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(106..819)
                     /locus_tag="VYQ30_001935"
     CDS             complement(106..819)
                     /locus_tag="VYQ30_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1966986.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1277..1651)
                     /locus_tag="VYQ30_001936"
     CDS             complement(1277..1651)
                     /locus_tag="VYQ30_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1966987.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
BASE COUNT          595 a          368 c          295 g          578 t
ORIGIN      
        1 agatgtaaat tcataattaa cagcttcatt aaaatcaact tcagacattt caatgtactc
       61 ttcaatagta atattagctt ttttgtagtc attagctcgt ctcctttaaa atgtgatctt
      121 ttcaagaata tttttatcta aacaatccag caagtcttgg taagaataga cttcgtaagt
      181 cggctgggct tgtgtgtgat tttcgaggtg atgaggatta taccagatag tgtcaatccc
      241 cgcattattg ccaccttgaa tgtcggcggt tagagaatct ccaatcatca gcgtcttttc
      301 tttactaaat ccagcaattt gctggccaat cttttcataa aaaagagcat ccggcttttg
      361 agtttgcaac tgttctgaga taaagacttg attgaaataa ggtgctagac cagattgagc
      421 caaacgtcct gtctgaatgg cagtaatgcc atttgtcgca gcatacaagt tataatcacg
      481 ctcaatgagg ctgtccaaga gatcatgagc gcccgatagt gtttgtccct gctgggcgag
      541 gtaaaattgg taacgctggg caagaaaact accgtctttt tcctgtccaa aatgagcaaa
      601 taaacgagaa aagcgcgtgt taaccagctc ttgtttactg attttcttca gctccaagtc
      661 tttccagaga gccttgttca taggaacgta ataatcttta taagcctgaa tatccgcaac
      721 tccttcttct tttagaagtt gggtcaaagc cacatcctca gcagcatcaa aatcaagaag
      781 agtgtggtcg aggtcgaaga gtagaaattt gtagaacaat ttgaggtttt cctttctgaa
      841 aattcattaa gaacattata tcataaagca cctcatacaa ttaactaatt taatcactta
      901 aaaaaattcg aacactttct atacaactga cagctcaaat ctttcagaat agaacaatac
      961 taactatcga acaccccgta ttcataaata tatatgtaat tctagaccta gaattcctat
     1021 aaactaaatg ctttcatact cttccaagta attgattgcc ttaaatttta atttttgaag
     1081 gtttctaaag ctagaatagc cccatcacaa tcagttttga ttgattcaca atttagaaac
     1141 actatagttt cactcctgtt aaaataaaaa ggaactgcat aaagcaatcc ctttctgatt
     1201 ttgaaatcat ttacttaaca ttttatagtt gagataatca atagcttacc tataaaaaga
     1261 gttatagtaa aattccttat ttattgattt caagctccgc taactgtgtt tgaataactg
     1321 acagttctgc accagcctga agaagagcag ctgcattata ggcaccttct acaattggaa
     1381 ccctgttgat gatgatactt ttatcactga aatcagtcac catttttaag ttcattttag
     1441 cagaacctag gtcaaaaaag gcaagtaaag tatctgctgg attttcggaa acaaccctat
     1501 ctacttgttc aaaactcgtt ccaattcctc cgccctcggt tcctcttaca taagtaatcg
     1561 gaacatcttt agctacttta ctaatcagtt caacaacacc ttctgcaatg tgtttggaat
     1621 gtgaaacgat aacaagacca atactttcca tcaaaccacc ccagtttcta aaatagcagt
     1681 aaagagtaat cctgatgaga atgatccagg atcaatatgt ccaagaaacc acatgctcct
     1741 aagacaagag ctaacagact ggccatcaat aatagtattg ttcttttttt catcattact
     1801 ccttaactag tgtttaactg attaattagc cagtaa
//
LOCUS       DAVMEF010000220         1827 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVMEF010000220 DAVMEF010000000
VERSION     DAVMEF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.221"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..939
                     /locus_tag="VYQ30_001937"
     CDS             <1..939
                     /locus_tag="VYQ30_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1966988.1"
                     /translation="LIIISLSFFILLIGDESYSFSTLIKVLNSETVPGASFSIMEIRL
                     PKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTT
                     GIISITCGLTSSLILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQE
                     VMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGANPELS
                     RLVLIFCAVSLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDI
                     FSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRNI"
     gene            939..1733
                     /locus_tag="VYQ30_001938"
     CDS             939..1733
                     /locus_tag="VYQ30_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1966989.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT          629 a          284 c          304 g          610 t
ORIGIN      
        1 ttaataataa tcagtctatc atttttcatt ttacttatcg gagatgaaag ttattctttt
       61 tcaactttga ttaaagtctt aaatagtgaa actgttcctg gagctagttt ttcgattatg
      121 gaaattagat taccaaaatt attagcagga attatagctg gctggtcttt tggattggca
      181 ggatttatct ttcaaactat gttaagaaat cctcttgcaa gtcctgatat aatcggtgtc
      241 acaagttctt catctattgc agcggtcttt tgcatattgg tattaaaaac aaatagttta
      301 actactggaa ttatttcaat aacttgtgga ctaacatcat ctttaatatt atttttacta
      361 gctaaaaaag atggtttttc agcagcaaga ctgataatat taggtattgg ttttcaagct
      421 gtcacaagag caggcacctc atttttattg ttgaaagtag caagatatga attacaagaa
      481 gttatgagat ggctcagtgg ctctttatct tttacaaagt tagatgacat acctcttgtt
      541 ctaatagtaa gtattattgc tactatatta gttttatttt ttaataaaag actagaaatt
      601 attgaacttg gtgaagaaat agcaatcgga cttggtgcaa atcccgagct ttcaaggctt
      661 gttttaattt tttgcgctgt atctttaact gctttttcta cttcaattac aggaccaata
      721 gcttgtatat cttttttagc tggtcccata gccttaaata ttggcaagaa aagaagccca
      781 atattagctg gattggttgg aattttacta gttttgttat cagacatatt ctctcaaaat
      841 attttaccag ctagatatcc agtaggtgtt gtaactggct tgttaggttc accatactta
      901 atatatttac taataaaaat gaacaggagg aatatataat gaaaggtctg tggtcaaata
      961 atcttacctg cggttatgat gaaaaaataa tcttggaaaa tataaatata aaaatacctg
     1021 aagaaaaaat atcagttatt attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa
     1081 ccttgtctcg acttataaag ccattagagg gagaagtatt gcttgataat aaatcaatta
     1141 attcttataa agaaaaagat ttagcaaaac acatagctat attacctcaa tctccaataa
     1201 tccctgaatc aataacagta gctgatcttg taagccgtgg tcgtttcccc tacagaaagc
     1261 cttttaagag tcttggaaaa gatgaccttg aaataataaa cagatcaatg gttaaggcca
     1321 atgttgaaga tctagcaaat aacctagttg aagaactttc tgggggtcaa aggcaaagag
     1381 tatggatagc tctagcccta gcccaagata caagtatcct acttttagat gagccaacta
     1441 cttacttgga tatctcatat caaatagaac tattagacct cttgactgat ctaaaccaaa
     1501 aatataagac aaccatttgc atgattttgc acgatataaa tctaacagca agatacgctg
     1561 attacctatt tgcaattaaa gaaggtaaac ttgttgcaga gggaaagcct gaagatatac
     1621 taaatgataa actagttaaa gatatcttta atcttgaagc aaaaattata cgtgacccta
     1681 tttccaattc gcctctaatg attcctattg gcaagcacca tgttaactct taataattga
     1741 atattggaat tcatttaaaa gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa
     1801 aactaactaa aggaggtcag tttttta
//
LOCUS       DAVMEF010000221         1823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVMEF010000221 DAVMEF010000000
VERSION     DAVMEF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.222"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(222..307)
                     /locus_tag="VYQ30_001939"
     tRNA            complement(222..307)
                     /locus_tag="VYQ30_001939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Leu,seq:aag)
     gene            complement(389..964)
                     /locus_tag="VYQ30_001940"
     CDS             complement(389..964)
                     /locus_tag="VYQ30_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1966990.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            complement(964..1752)
                     /gene="codY"
                     /locus_tag="VYQ30_001941"
     CDS             complement(964..1752)
                     /gene="codY"
                     /locus_tag="VYQ30_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1966991.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT          568 a          404 c          308 g          543 t
ORIGIN      
        1 ggcaagatga ccaaaaaaat aaacaagttc tgtgcaactt gcacaaaact ttagagaata
       61 tcggagaatt tgaagaagat aatctttatt attcttctat agacaaaagc agaaacaagg
      121 atcagttttc ccatattttc gctgattctc cactacattt gactggattc taatttttta
      181 gagaaacaca aaagagctag ctttagctag ctcttttcct atgcggagag agggacttga
      241 accctcacga cctaaagcgg tcacaggatc cttagtcctg cgcgtctgcc aattccgcca
      301 tccccgcgtc gattacttta ctagtatatc aacttttggg atgcttgtca acactttttt
      361 tcaaattttt tcattttcac caaccaggtt actcaaaaag ttcatttaga ttttcatcta
      421 ctaacttagc tccgagtgta tttttgaaat gacctagggc aaattgatga ttttcaggcc
      481 agatggaagc aacagctggt ttaacaatct cgatgtcata tcctagatta taggcatcta
      541 tagctgtatg taggacacag atatccgtca agacacctgt taagataacg gtagacactc
      601 tacgctctct caaacgaata tctaggtcag tccctgaaaa agctgagtaa tggcgtttat
      661 ccatccaaaa gacacgactg tctgaaccat gctcttgata aaagatcccc aaatctccat
      721 ataaattccg tccactcgtc ccaatcagat tatgaggagg aaataactta ctttccggat
      781 ggaaacaatc gttttcttca tgagcatcaa tagtaaagaa gatataatct cctcgttcaa
      841 aagctaatcg agttaccttg ctgatggcat ccgaaatcgc ctgagctgga gcacctgctg
      901 ttagtttccc actatcagca acaaaatctt ctgtataatc aatcgaaatt aaagcctttg
      961 tcattagtaa tctcttttct tcacttcttc aaaaatatct gaaatcaaga ccttaagata
     1021 ggttcccttc attccaagtg agcgactttc aataatcccc gcagactcaa gtttacgaag
     1081 agcattgaca atcacagagc gagtgattcc gatacgatct gcaatcactg acgcagtcaa
     1141 cttcccttca tttccattta attcccctaa aattgctgaa acagcacgga gttcggagta
     1201 agaaagggta ttgaccgcca tggtgacagc agtacgacga cgaatatttt tctcatcttc
     1261 ttcacgttgg aagttaagaa gctgaatccc aacaacggta ctggcaatct caacaagaac
     1321 caagtcctca tcttcgaatt ttttatcatt acgccaaata atcaaagaac caaggcgaat
     1381 ccccgataca tgaatcggtg caatagtcgt caagccatct ggaaaatcat ctctactctc
     1441 aacagggaaa atactcatat catgctcaac aggcaagttt gcttctgttt cgtaaatcat
     1501 attagcccct tgaacgtagt catctgggaa aatcttagtt tggaagaatt gctctacgcg
     1561 atctgtattt gttttataac gcataaaata gccaagcaga cgtcccttac tattgataat
     1621 gcaggcattg caatgaataa tatccgctaa ctgacgcgta atagcgttgt aagggagctc
     1681 atcctgcaac tgctcctctg agcgtttcaa aattgatgta atttttctag ttttttctaa
     1741 taaatgtgcc atttttcacc tcgaatttaa tcgctatcat tataacataa aaacgtctct
     1801 ttttcaataa ttatctgaaa att
//
LOCUS       DAVMEF010000222         1814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVMEF010000222 DAVMEF010000000
VERSION     DAVMEF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.223"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            53..1684
                     /locus_tag="VYQ30_001942"
     CDS             53..1684
                     /locus_tag="VYQ30_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1966992.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          521 a          383 c          363 g          547 t
ORIGIN      
        1 ctttaaaata gaataggaga tattccgcga ttatttttcg gaggaaaaag aaatgtccat
       61 taattggcag gaaattttat ttcacttttt aggtggtctg gggctattct tatatagtat
      121 caagaccatg ggagacggtt tacaacaagc tgctggagat cgccttcgtt tttacattga
      181 caaatatact agtaatcctt tctttggagt tctggttggt attgggatga ctgctctaat
      241 tcagtctagt tctggtgtaa cagttatcac agtcggcctg gtcagtgccg gtctcttaac
      301 cttacgtcag gctatcggga ttgtcatggg tgctaatatt gggacaactg tcacatcctt
      361 tctcatcggt tttaaattag gtaactatgc cctacctatg ctctttatcg gtgccgtctg
      421 tctctttttt acaaaaaatc ggacagtcaa taatatcgga cgcatcctct ttggtgtcgg
      481 tggtatcttt tttgccctca atctcatgag cggcgcaatg gctccactca aggatttaca
      541 ggtctttaag gactatatga ttgagctaag taaaaatcct gttttgggtg tctttgtcgg
      601 tactggcttg accttgctaa ttcaagcttc ttcggctacc attgggattt tacaaaacct
      661 ctacgccggc aatctaattg acctacaggg agctttgccg gttctatttg gtgacaatat
      721 cgggacaacc attacagcca tcattgcctc tttaggggct aatattgcag ctaaacgggt
      781 agcaggagct catgttgcct tcaacgttat cggaacagtc gtctgcgtta tttttctagt
      841 tccttttact gtcctgattc attggtttga agctacgcta aatctagcac cggaaatgac
      901 catcgccttt gctcacggaa cctttaatat taccaacacc attgtccaat ttccatttat
      961 cggagctctg gcttactttg taaccaagat tattcctgga gaggacgagg ttgtcaaata
     1021 cgaaccctta tatcttgatg aacatttcat caaacaggcc ccatctatcg ctctaggaaa
     1081 tgctaagaaa gagctcttgc acttaggaaa ctacgctgct aaagcctttg acctttccta
     1141 taagtacatc attgacttgg atgaaaaagt tgctgaaaaa gggcataaaa ccgaagaagc
     1201 aattaacacc atcgatgagc aattaacacg ttatctcatt gccctttcaa gcgaagctct
     1261 cagccaaaaa gaaagtgaag tgcttaccaa tatccttgat tcctcccgtg atttggaacg
     1321 gattggagac cacacggagg ctctactcaa tctgactgac tatcttcaac ggaaaaatgt
     1381 tgaattttct gatgccgcct tgaaagaatt agaggaagtt taccgccaaa ctagtgactt
     1441 tatcaaagat gctctggata gtgtggaaaa caatgatatt gaaaaagcac gcagtcttgt
     1501 agaacgtcat gaagcaatca ataagataga acgtgttctc agaaaaaccc acatcaaacg
     1561 cctcaacaaa ggcgaatgtt caacacaagc tggggtcaac tttatcgaca tcatctcaca
     1621 ctacactcgt gtatcagacc acgctatgaa ccttgctgaa aaggtttttg cagaacaaat
     1681 ctaagaacca agaagctatc catcataatt ggatggcttt ttactttttc ctaagcaaga
     1741 ctaggctgaa tgaaactgaa agagtattct gcagatatat agtatattga aactagaata
     1801 gtacacctct attt
//
LOCUS       DAVMEF010000223         1810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVMEF010000223 DAVMEF010000000
VERSION     DAVMEF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.224"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..>1810
                     /gene="phtE"
                     /locus_tag="VYQ30_001943"
     CDS             33..>1810
                     /gene="phtE"
                     /locus_tag="VYQ30_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1966993.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYT"
BASE COUNT          634 a          334 c          354 g          488 t
ORIGIN      
        1 aaaacttcat catttattga aatgagggat ttatgaaatt tagtaaaaaa tatatagcag
       61 ctggatcagc tgttatcgta tccttgagtc tatgtgccta tgcactaaac cagcatcgtt
      121 cgcaggaaaa taaggacaat aatcgtgtct cttatgtgga tggcagccag tcaagtcaga
      181 aaagtgaaaa cttgacacca gaccaggtta gccagaaaga aggaattcag gctgagcaaa
      241 ttgtaatcaa aattacagat cagggctatg taacgtcaca cggtgaccac tatcattact
      301 ataatgggaa agttccttat gatgccctct ttagtgaaga actcttgatg aaggatccaa
      361 actatcaact taaagacgct gatattgtca atgaagtcaa gggtggttat atcatcaagg
      421 tcgatggaaa atattatgtc tacctgaaag atgcagctca tgctgataat gttcgaacta
      481 aagatgaaat caatcgtcaa aaacaagaac atgtcaaaga taatgagaag gttaactcta
      541 atgttgctgt agcaaggtct cagggacgat atacgacaaa tgatggttat gtctttaatc
      601 cagctgatat tatcgaagat acgggtaatg cttatatcgt tcctcatgga ggtcactatc
      661 actacattcc caaaagcgat ttatctgcta gtgaattagc agcagctaaa gcacatctgg
      721 ctggaaaaaa tatgcaaccg agtcagttaa gctattcttc aacagctagt gacaataaca
      781 cgcaatctgt agcaaaagga tcaactagca agccagcaaa taaatctgaa aatctccaga
      841 gtcttttgaa ggaactctat gattcaccta gcgcccaacg ttacagtgaa tcagatggcc
      901 tggtctttga ccctgctaag attatcagtc gtacaccaaa tggagttgcg attccgcatg
      961 gcgaccatta ccactttatt ccttacagca agctttctgc cttagaagaa aagattgcca
     1021 gaatggtgcc tatcagtgga actggttcta cagtttctac aaatgcaaaa cctaatgaag
     1081 tagtgtctag tctaggcagt ctttcaagca atccttcttc tttaacgaca agtaaggagc
     1141 tctcttcagc atctgatggt tatattttta atccaaaaga tatcgttgaa gaaacggcta
     1201 cagcttatat tgtaagacat ggtgatcatt tccattacat tccaaaatca aatcaaattg
     1261 ggcaaccgac tcttccaaac aatagtctag caacaccttc tccatctctt ccaatcaatc
     1321 caggaacttc acatgagaaa catgaagaag atggatacgg atttgatgct aatcgtatta
     1381 tcgctgaaga tgaatcaggt tttgtcatga gtcacggaga ccacaatcat tatttcttca
     1441 agaaggactt gacagaagag caaattaagg ctgcgcaaaa acatttagag gaagttaaaa
     1501 ctagtcataa tggattagat tctttgtcat ctcatgaaca ggattatcca agtaatgcca
     1561 aagaaatgaa agatttagat aaaaaaatcg aagaaaaaat tgctggcatt atgaaacaat
     1621 atggtgtcaa acgtgaaagt attgtcgtga ataaagaaaa aaatgcgatt atttatccgc
     1681 atggagatca ccatcatgca gatccgattg atgaacataa accggttgga attggtcatt
     1741 ctcacagtaa ctatgaactg tttaaacccg aagaaggagt tgctaaaaaa gaagggaata
     1801 aagtttatac
//
LOCUS       DAVMEF010000224         1806 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVMEF010000224 DAVMEF010000000
VERSION     DAVMEF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.225"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            56..715
                     /locus_tag="VYQ30_001944"
     CDS             56..715
                     /locus_tag="VYQ30_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1966994.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAKLTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            740..>1806
                     /locus_tag="VYQ30_001945"
     CDS             740..>1806
                     /locus_tag="VYQ30_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1966995.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDAKEMPKDWT
                     ELASKYKGQYSIYGLTGGTGRAIYASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESTVVKVLDKENAIQYGMIWGSGALVGQKEQNVVFKVMSPEIGVPFVTEQTMILS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
BASE COUNT          652 a          258 c          360 g          536 t
ORIGIN      
        1 ttgtataatg taaacatgtg acaccgatgt caaatataaa aaagaaggtc tttatatgaa
       61 aaacttaaca aaaattaagt tcaaagagaa tggggaattt aatcatttcc ctgggaatac
      121 agttgtagca aatctttata ctaaacaaga tttgatggaa gttgttgata ttattcaatc
      181 acgttataga gaattaccat ttatcgataa gtttacttta actccaagga attctattca
      241 catgactgta attgaattgt tgtgccatga aaatcgtgaa acggagtttt ggagcagtaa
      301 tcttcctcta gatacacctt tacaggaaat acatgattac tttgctaaac aacttgaaat
      361 ttttccattg ttggatgaag aaattcatat gcgtataact gaaatgggaa aacaaaacat
      421 actagttgaa cctgcagatg aagcttctgc aaagagatta gaagaaattc gtacttatgt
      481 ctcagaaaaa gcaggtgttt gtttccctaa tcatgataga tatcaattcc atatttcaat
      541 tgggtatctt cggattcctc taaccgaaga ggaggaagaa gagtttacta aagtcagagc
      601 aaaattaact gaaattttat tagagaagat tccaactatt actgtaaacc gtattgacta
      661 tactgtattt gaagatatga gacaatttgt tccatatcac gaaaaattta aataaaataa
      721 taaaaaagga gaacttatca tgaaaaaaat gaaagtttgg tctactgtac ttgcaacggg
      781 agttgctctt actacacttg ctgcttgctc tggaggttca aattctacga ctgcttcttc
      841 atctgaagaa aaagctgata aaagtcaaga attagtaatc tattcgaact cagtctcaaa
      901 tggtcgtggt gattggttaa ctgctaaagc aaaagaagct ggttttaata taaaaatggt
      961 tgatatcgct ggcgctcaat tagcagaccg tgttattgct gagaagaata atgcagttgc
     1021 agatatggta tttggaattg gtgctgttga ctcaaataaa attagagatc aaaaattact
     1081 agtacagtac aagcctaaat ggttagataa aattgatcaa tctttatcag ataaagataa
     1141 ttattataac ccagttattg ttcaaccctt ggtattgatt ggagctcctg atgcaaaaga
     1201 aatgccaaaa gactggacag agttagctag taaatataag ggacaatatt ccatttatgg
     1261 cttaactggt gggactggac gcgccattta cgcaagtatt ctagttcgtt atcttgatga
     1321 aaaaggtgat ttaggagtat cggaaaaggg ctgggaagtt gcaaaagaat actttgcaaa
     1381 tgcctataca ctacaaaaag gtgaaagcac agttgttaaa gtattagaca aagaaaacgc
     1441 tatccaatac ggtatgatct gggggtccgg ggctctagtt ggtcaaaaag agcaaaatgt
     1501 tgtttttaaa gtgatgtctc ctgaaattgg ggttccattt gtaactgagc aaacaatgat
     1561 tttgagtaca agtaagaagc aagcattggc taaagaattt attgattggt ttggtcaatc
     1621 tgaaattcaa gtagagtaca gtaaaaactt tggctctatc cctgccaata aagatgccct
     1681 caaagagcta ccagaagata ctaagaaatt tgttgatcaa gtgaaaccac aaaatattga
     1741 ctgggaagct gttggaaagc atttggatga atgggtagaa aaagctgaat tagaatacgt
     1801 aaaata
//
LOCUS       DAVMEF010000225         1799 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVMEF010000225 DAVMEF010000000
VERSION     DAVMEF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.226"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..823
                     /locus_tag="VYQ30_001946"
     CDS             17..823
                     /locus_tag="VYQ30_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1966996.1"
                     /translation="MNKMKKVLMTMFDLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKDSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKLGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            789..1259
                     /locus_tag="VYQ30_001947"
     CDS             789..1259
                     /locus_tag="VYQ30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1966997.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVVKKANEAKK"
     gene            complement(1548..>1799)
                     /locus_tag="VYQ30_001948"
     CDS             complement(1548..>1799)
                     /locus_tag="VYQ30_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1966998.1"
                     /translation="LSTLYLYNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVRIGIC
                     RDDAKLIQKGFSLLELTEETSILSHLKKEVEIYYQAKER"
BASE COUNT          573 a          302 c          408 g          516 t
ORIGIN      
        1 taaaggaggc tttattatga ataagatgaa gaaggtgttg atgacgatgt ttgatttagt
       61 gatgctcccc ctactatttg cttgtagcaa caatcaatcg gctggaattg aagccatcaa
      121 gtccaaagga aaattggttg tagccctcaa tccagatttt gctccatttg aatatcaaaa
      181 agtggttgat gggaaaaatc agattgtggg ttcagatatc gaattagcca aggctatcgc
      241 aacagaacta ggtgtcgaat tggaactatc tcctatgagt tttgacaatg tactggctag
      301 cgttcaatca ggaaaagctg accttgccat atcaggtgtt tctaagacag atgaacggag
      361 caaggtgttt gatttttcaa ctccctacta tactgcaaaa aataaactca ttgtcaaaaa
      421 atctgattta gccacttatc agtctgtcaa cgatttggcg cagaaaaagg tcggagcgca
      481 gaaagattcg attcaagaga cgatggcgaa agatttgcta caaaattctt ccctcgtatc
      541 tctgcctaaa aatgggaatt taatcacaga tttaaaatta gggcaagtgg atgccgttat
      601 ttttgaagaa cccgttgcca agggatttgt ggaaaataat cctgatttag caatcgcaga
      661 cctcaatttt gaaaaagagc aagatgattc ctatgcggta gccatgaaaa aagatagcaa
      721 ggaattgaaa gaggcagtcg ataaaaccat tcaaaagttg aaggagtctg gggaattaga
      781 caaactcatt gaggatgcct ttaaagcgtc cattgaaaaa tagaaagaag gaaaagaaca
      841 tgagtaaaga aaaagtaatt ttggcctatt ctggcggttt agacacatca gttgctatta
      901 cctggctgaa gaaagactat gatgttgttg cagtttgtat ggatgtgggt gaagggaaag
      961 acttagattt catccatgat aaggctctta aggttggggc agtcgaatct tatgtcattg
     1021 atgttaagga cgaatttgct acagattatg tgctagtagc tctccagtca catgcctact
     1081 atgaacaaaa gtaccccttg gtatctgcct tgagccgccc tctgatttct aaaaaattgg
     1141 ttgaaatagc gcatcagata ggagcgacta caattgctca tggttgtacc ggtaagggga
     1201 atgaccaagt cgaataccag attgcagtcg ttaagaaagc gaatgaagct aaaaagtgat
     1261 ggagtgatgg gttcagaata agaggttggt caattggcag cctctttctt gtcgttgaaa
     1321 aagtgagata tattgacttt tgaaaaaaat gtcataattg agttgggata aacgtaaaag
     1381 tcatactttc tcgttaaatc gttcggcgat tcatggtgac atggacacat agtagtaaac
     1441 acactatgtg gcttggcaga gctaaaaact tctggcttaa caatggctgg tattaagaac
     1501 taacgaacaa ataatatgta aagagtcgag gaaatccctc ggctttttta tctttccttc
     1561 gcttggtaat agatctctac ttcttttttg agatgagaca gtatagaagt ttcctcggtt
     1621 agctccagaa gggagaaccc tttttggata agtttcgcat catccctaca aatcccaata
     1681 cggacatagc agatagccag cctatcgtag cttttcttat tcttggcgtc ctccagtaaa
     1741 gtgtagcaga tttgcttaca catgttttta tcttgattgt atagatagag tgtggaaag
//
LOCUS       DAVMEF010000226         1763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVMEF010000226 DAVMEF010000000
VERSION     DAVMEF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.227"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1763
                     /locus_tag="VYQ30_001949"
     CDS             <1..>1763
                     /locus_tag="VYQ30_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEV1966999.1"
                     /translation="SVQNTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQF
                     KVVCHSKVTDGEVSVLGETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFT
                     EKDEHVKKYQEQFNRVDFKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLI
                     SSSQPNGLPANLQGIWCDELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLE
                     RMREPGRLTAKKMYGARGFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHY
                     LYFQDERILTEHFEMIKEAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACL
                     SSTIDNQILRYFCDSCIGIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDY
                     EEVEPGHRHISPLFGLYPYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAIN
                     NWLVSGLHASTQTGWSAAWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQI
                     DGNLGLVSGICELLVQSHHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDIT
                     FLKLEGGNKDQKVRVRIYGKN"
BASE COUNT          607 a          237 c          363 g          556 t
ORIGIN      
        1 catcagttca aaacacatta aatttaaaca ttaatttggg tagaaataaa cggtttaatg
       61 atgaagtatc taaactggat tcaagtacaa ttttaatgtc ggcctctgct ggaggtagaa
      121 aaggtgttca gtttaaagta gtatgtcatt ctaaggttac ggatggtgaa gtaagtgtat
      181 tgggagagac aatagttatt cggaatgcta cagaggtatt tctttatctc aaatcaatga
      241 cggattattg gggaaatata gatatttctt ctcttcaggg agaatttagt agtattgatt
      301 actttacaga aaaagatgaa catgtaaaaa aatatcagga gcaatttaat agagttgatt
      361 ttaaactaga ctatagtaaa gattgtctta gcattccaac gaatctactt cttgaaaaca
      421 ctaaaaagta tagtaactac ttgactaact tgttatttca ttatggaaga tatctgttaa
      481 tatcgtctag tcaaccgaat ggtttacctg ccaatcttca aggaatatgg tgtgatgaat
      541 taaatccaat ttggggttct aaatatacga ttaatattaa tactcaaatg aattattgga
      601 tggtaggtcc atgtgattta ccagaagtag aatatccatt atttgatatg ctcgaaagaa
      661 tgagagaacc gggaagacta accgctaaga aaatgtatgg agctagaggt tttacagcac
      721 atcataatac ggatggtttt ggcgatacgg ctccccaatc tcatgccatg ggggctgcaa
      781 tttgggtatt aactattcca tggttatgta ctcatatttg ggaacactat ttatatttcc
      841 aagatgagcg tattcttacg gaacattttg aaatgataaa agaagcattt cttttctttg
      901 aagattattt atttgaggtg gatggctact tgatgacagg tccaagtgtc tcaccggaaa
      961 ataaatatcg cttaaaaaat ggtattgaag gaaatgcttg tctatcatct acaattgata
     1021 atcaaattct aagatatttt tgtgattcat gcattggcat tgcaaaacaa ttaggagaca
     1081 attcggattt tattagtcgt gtgaaggagt taaaaaagaa actacctaaa acaaaaatag
     1141 gtagtaatgg gcaaatccaa gaatggttag aagattatga agaagtagag cctgggcata
     1201 gacacatttc acctctattt gggctttatc cttataatga gattgatatt cataaaactc
     1261 cggaattagc agaagcagct aaaatcacta tcaataggag attatcaaac gctaattttt
     1321 tatcttcaca ggagagggag caagcgatta ataattggtt agtaagtggt ttgcatgcta
     1381 gtacacaaac aggttggagt gctgcatggc tgattcattt ttttgcgaga ctatatcaag
     1441 gtgaacctgc ttataaccag attaatggtt tgttaaataa tgcgactctt ggcaatttat
     1501 ttcttgacca tccaccattt caaattgatg gtaatttagg tttggtgagt ggaatttgtg
     1561 aattattagt acagagccat cataattggt tatcactaat tccagcttta ccttctgctt
     1621 ggtcagaagg agaagtgaaa ggtttcagag taagaggagg atataaggta tcgtttgctt
     1681 ggaaaaatgg ggatataaca ttcctaaaat tggaaggagg aaacaaagat caaaaagtaa
     1741 gagtaagaat atatggcaaa aat
//
LOCUS       DAVMEF010000227         1739 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVMEF010000227 DAVMEF010000000
VERSION     DAVMEF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.228"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(860..1729)
                     /locus_tag="VYQ30_001950"
     CDS             complement(860..1729)
                     /locus_tag="VYQ30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1967000.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT          573 a          353 c          326 g          487 t
ORIGIN      
        1 tttccttact attttatcaa atttcagcta aattgcaagt gttttacaac gtttttgtga
       61 attattcttg gtaaaaattt aactaactaa tagaaagtac taaatagact tatccttcaa
      121 ggagttttca gcattatcca tccactcact tgctgaaaat gtaaacagtc tctggtttat
      181 atagtaaaat gaaacaagaa tagggcaaat cgatcaggac aatcaaatcg atttctaaca
      241 atgttttaga agttgaggtg tactattcta gtttcaatct actatacaac ctcaaaactg
      301 tgttttgaac aacctgcggc tagcttctta gtttgccctt tgattttcat tgagtataaa
      361 attgcagata aaacgataac aatcatagga ctcagcagag tcaatgtagg tcgaagaata
      421 gccccctaat cgcttatttt atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa
      481 ggtaaatcaa ttcctcacaa tgtttgagaa agaagggagg actattccaa cttcaaccca
      541 ctataaagca gacttacaag atagggaacc catctccgat aatattccga taatatatag
      601 tagaatgaaa actgcacaat ttgattggag aattcaaacc aatttctaac aatgttttag
      661 aaacaagggt ctattatttc aactgcaacc tactatagcc cattgaagct ggaatagccc
      721 actgagactt ctaaaccact gctggagagg agtttgttta gagtttcttt catttcccta
      781 aaaaatcaaa aagagagaac cagtctgatc ctctcttact tatagatggt gctcactcaa
      841 atgttaaagg agctatttct tagagataaa ggaatttgaa tacagctact gccgcaattg
      901 ctgctgcgat aggtgctact actggtaccc aagaatacca ccattttgaa tcgcctttat
      961 gctcaccaag aactgatttt ggaaggaaag catgaaggag acgtggtccc aagtcacggg
     1021 ctgggttcaa ggcaggtcct gtaggtcctc caagtgatgt taccaaagcc ataacgagga
     1081 atccaagtgc caagtgagcc acagaaagtc ctgaagcagt gtgtggagcc acctgtgctt
     1141 taatagccaa gtcagaaaaa tcaactgttt gtcctgcttc tattgccttt tgtttcatga
     1201 attgaagcac ttcagcacca aagaagtttt tagtcaaacc aagagctgca aagaacaaaa
     1261 caaatgaacc aacaaactca ttaatcaaac cattgacagt tgctgcatag cgactttctt
     1321 ttgtaccatg gtcaatactt gaaatagttg agaaagttcc caagatgtta tttggttttt
     1381 cagttttcaa gtagaatgga cggtatgttg ccacaactaa ggcttggcca aagatagccc
     1441 ccaagacttg cgcgataatg taaggtacca cttgtgccca agggaaaaga ccgctaactg
     1501 caagccctag agtgaaagca gggttgatgt gattcccaga tacgttacca aacatcaagg
     1561 ctgggatcat aacccccata ccataaccaa cagcgatgac gatccagcca ctttggtgac
     1621 ctttcgtacc tttaagttca acgttggcaa ctgcaccatt cccaagaatg atcaaaatgg
     1681 cagttcccaa aaattcagtg gcatacttca gtgcccatgt gaaatccatt tgctagatt
//
LOCUS       DAVMEF010000228         1699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVMEF010000228 DAVMEF010000000
VERSION     DAVMEF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.229"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            128..655
                     /locus_tag="VYQ30_001951"
     CDS             128..655
                     /locus_tag="VYQ30_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1967001.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            667..1182
                     /locus_tag="VYQ30_001952"
     CDS             667..1182
                     /locus_tag="VYQ30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1967002.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1179..1646)
                     /locus_tag="VYQ30_001953"
     CDS             complement(1179..1646)
                     /locus_tag="VYQ30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1967003.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT          478 a          362 c          316 g          543 t
ORIGIN      
        1 actattctag tttcaatcta ctataacttt taactaaaaa atgatctata aacccaagta
       61 aattcttgct ttattgtctg gtttattgta ccatactagt gtatatgcag ttaaaaagga
      121 gattcttgtg aatacacgga aaaagacaca atttatgaca atgacagccc ttttaacggc
      181 tattgcgatt ttgattccaa ttgttatgcc tttcaagatt gtcattccac ctgcttccta
      241 tactttgggg agccacatcg ctatttttat agccatgttc ttgtcgccct tgatggcagt
      301 ttttgtcatc ctagcctcta gttttggatt tttgatggct ggctatccca tggttatcgt
      361 ttttcgggct ttttcccata tatcttttgg tgctttagga gctctttacc tacaaaaatt
      421 ccccgatacc ctagataaac caaaatcttc ctgggttttc aattttgttt tggctgttgt
      481 tcatgccctt gctgaagtat tagcctgtgt ccttttttac gcaacttcgg gtaccaatgt
      541 agaaaatatg ttctatgttc tatttgtact agttggattt ggcacaatta tccatagtat
      601 ggtagactat acattagcac tagctgtcta taaagtgctt cgaaaacgcc gttaaaagga
      661 aaagctatga caaaagatcg caaacaagcc ctgctccaac tcttgaaaga agctcctaaa
      721 gcccttaatg gccaaagttt ggcggaacat tttcatgtca cacgtcaggt cattgtgcag
      781 gacattgcaa ttttgagagc cgatggcgct cctatcctat ccaccaatcg tggctacatc
      841 tataagcaaa ttgataccaa tccttatgtt cacaaacttt tcaaagtgaa acatgaagtt
      901 gaagaaatcg gtcaggaact ccttgctatt gtcgataatg gtgggcatgt ccagaatacc
      961 ttgattgacc atcccgttta tggagaaatt gaaaccttgc ttaaactgtc ttgtcgcaga
     1021 gatgtgcaac attttctaga acaagtcgag cattctgact ttagacctct atctgaattg
     1081 acagatggca tccattacca cctagtcgaa gccgaaacac aacaagacct ccactatatc
     1141 gaggaggcct tggatcagct aggttattta gtaaaagact agaagatttt cttttcccaa
     1201 tcgtcttctg tacggcgagg atagaaaaac tctgacttgt cgggagcttc catcatttcc
     1261 atcaatggta gcatatcata ggccagattt aagtttggaa tctggtcttt ttgcacccaa
     1321 gaaacttctc cctcttctga agattgaagg gtaccagaga actcagtcgc cttataacaa
     1381 atgacaatat agcgcccacc tgtatctagt ggccaatttt taatgccgac aagttgagga
     1441 ttttggatag tcaaccctgt ttcttcgtag atttcacgaa tgacagactc cgcaaaagcc
     1501 tcatcatttt ctacatgacc tccaggaaag gcataaccag accagcgatt gttttcaggg
     1561 gcgcgatact gcatcaccac gcgctgagtt tcgaggtctt caatcagaca gatatttgtt
     1621 aaaattgtta attgggaacg ggacataaat ttactcgctt tctaatttat taaattttca
     1681 gacttactac cagagatat
//
LOCUS       DAVMEF010000229         1680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVMEF010000229 DAVMEF010000000
VERSION     DAVMEF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.230"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..585)
                     /locus_tag="VYQ30_001954"
     CDS             complement(<1..585)
                     /locus_tag="VYQ30_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967004.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSEITDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGM"
     gene            complement(641..1663)
                     /locus_tag="VYQ30_001955"
     CDS             complement(641..1663)
                     /locus_tag="VYQ30_001955"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1967005.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          489 a          377 c          299 g          515 t
ORIGIN      
        1 cattccttta aagatatatg gttccccctt aataccaact tgtgagtatg gttttgagaa
       61 tggtactacg ttacttacaa ctggaaaacc accagatgaa gctgttggca tcaataatga
      121 actatctgtc gaccaagctt gagctttggc atatttttca tatctcttct ctaggtcagt
      181 gatctcagaa acagcatctt ctaacaattt cttatattta tccaaaccag gtttagctac
      241 aacatcctta tcttttcctt tcgtaatgcc aaggtgtttc atggcagaac cagattttgg
      301 atccataata ttcaagtaag acgctggatc ttgatagctt ggagcccatc ctgtactgtt
      361 caaatcatag tctttttgag aaggagcaac attgccgtat ttatcatttt ccatcaaacc
      421 atcaataaca tttccaataa cgtctgtcct cgatgttcga gtcgctatac tgtagcccaa
      481 tgatgctgga tctactgcat agacataaga aaatgttgtc ggtgcatctg cttctttatc
      541 agtttttcca caagccacta aaatagctga cgtgctcagg accactcctg ctgttaagag
      601 ccactttttc tatttcataa aaaatctcct ttggtttatt ttaatctact tttacaatcc
      661 aaccttctgg cgcttcaata tcgccaaact gaatacccgt caattcatta tataatttac
      721 gcgtcacagg acctacttct gtttcactat agaatacatg gaaatcatca ccatgttgaa
      781 tacctccaat tggagaaata accgctgctg taccacaggc acctgcctct acaaaacggt
      841 caagattatc aattggaaca tcaccctcaa taggagttaa tcccaagcga tgttctgcca
      901 aataaagcaa ggaatacttg gtaatagatg gcaagataga tggactcaat ggtgttacaa
      961 attcattatc agctgtaatt ccaaagaagt tagctgatcc gacttcttca atctttgtat
     1021 gagttgatgg gtccagatag ataacatctg agaaatgacg tgacttggcc atttttcctg
     1081 gtaagagact tgcagcatag tttccaccaa ccttagccgc acctgtacca tttggtgctg
     1141 cacggtcgta ctcatcctga atcaagaagt tggttgggac caaaccacct ttaaagtaat
     1201 ttccaactgg catagcaaag atggtgaaaa tgtactcttc tgccggtttt accccgataa
     1261 tatctccgac accaatcaaa agagggcgaa gatataaggt tccacctgtt ccgtatggtg
     1321 gtacgtattc ttcattcgca cggacaactg ctttacaagc ttctacaaac atgtctgtcg
     1381 gaacttgtgg catcaagaga cggtcacatg tacgttgcag acgtttagca ttttcatcag
     1441 gacggaacag ttgaacactg ccatccttag tacgataagc tttcaaacct tcaaatgctt
     1501 gttgtccata gtgaagactt ggagaagact ctgaaatatg caaagttgca tcctctgtaa
     1561 gctctccttg atcccattgt ccatttttga aatgagcaag atagcgataa ggtaatttca
     1621 tataggaaaa accgaggttt tcccaatcaa tcgttactgt catattccac tccttattct
//
LOCUS       DAVMEF010000230         1671 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVMEF010000230 DAVMEF010000000
VERSION     DAVMEF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.231"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..920
                     /gene="adcAII"
                     /locus_tag="VYQ30_001956"
     CDS             3..920
                     /gene="adcAII"
                     /locus_tag="VYQ30_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1967006.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            928..>1671
                     /locus_tag="VYQ30_001957"
     CDS             928..>1671
                     /locus_tag="VYQ30_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1967007.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNE
                     LSASELAAAEAYWNGKQGSRPSSSSSYNAN"
BASE COUNT          557 a          298 c          373 g          443 t
ORIGIN      
        1 tcatgaagaa acaaaattta tttttagtcc tgttaagtgt ctttcttttg tgcttggggg
       61 cttgtggtca aaaggaaagt cagacaggaa aggggatgaa aattgtgacc agtttttatc
      121 ctatctacgc tatggttaag gaagtatctg gtgacttgaa tgatgttcgg atgattcagt
      181 caagtagtgg tattcactcc tttgaacctt cggcaaatga tatcgcagcc atctatgatg
      241 cagatgtctt tgtttaccat tctcatacac tcgaatcttg ggcaggaagt ctggatccaa
      301 atctaaaaaa atccaaagtg aaggtcttag aggcttctga gggaatgacc ttggaacgtg
      361 tccctggact agaggatgtg gaagcagggg atggagttga tgaaaaaacg ctctatgacc
      421 ctcacacatg gctagatcct gaaaaagctg gagaagaagc ccaaattatc gctgataaac
      481 tttcagaggt ggatagtgag cataaagaga cttatcaaaa aaatgcgcaa gcctttatca
      541 aaaaagctca ggaattgact aagaaattcc aaccaaaatt tgaaaaagcg actcagaaaa
      601 catttgtaac acaacataca gccttttctt atctagcgaa gagatttggg cttaatcaac
      661 ttggtattgc aggtatctct cctgaacaag aaccaagtcc acgacaacta acagaaattc
      721 aggaatttgt taagacctat aaggttaaaa cgatttttac agaaagtaac gcttcttcaa
      781 aagtagctga aactcttgtc aaatcaacag gtgtgggtct taaaactctg aatcctttag
      841 agtcagaccc acaaaatgac aagacctatt tagaaaatct tgaagaaaat atgagtattc
      901 tagcagaaga attaaagtga ggaaagaatg aaaatcaata aaaaatatct agcaggttca
      961 gtggcagtcc ttgccctaag tgtttgttcc tatgaacttg gtcgtcacca agctggtcag
     1021 gttaagaaag agtctaatcg agtttcttat atagatggtg atcaggctgg tcaaaaggca
     1081 gaaaacttga caccagatga agtcagtaag agggagggga tcaacgccga acaaatcgtc
     1141 atcaagatta cggatcaagg ttatgtgacc tctcatggag accattatca ttactataat
     1201 ggcaaggtcc cttatgatgc catcatcagt gaagagctcc tcatgaaaga tccgaattat
     1261 cagttgaagg attcagacat tgtcaatgaa atcaagggtg gttatgttat caaggtagat
     1321 ggaaaatact atgtttacct taaggatgca gctcatgcgg ataatattcg gacaaaagaa
     1381 gagattaaac gtcagaagca ggaacacagt cataatcacg ggggtggttc taacgatcaa
     1441 gcagtagttg cagccagagc ccaaggacgc tatacaacgg atgatggtta tatcttcaat
     1501 gcatctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg cgaccattac
     1561 cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga agcctattgg
     1621 aatgggaagc agggatctcg tccttcttca agttctagtt ataatgcaaa t
//
LOCUS       DAVMEF010000231         1625 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVMEF010000231 DAVMEF010000000
VERSION     DAVMEF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.232"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            35..490
                     /locus_tag="VYQ30_001958"
     CDS             35..490
                     /locus_tag="VYQ30_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1967008.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVHAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYSNPAYLY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWQKQHKIG"
     gene            655..954
                     /locus_tag="VYQ30_001959"
     CDS             655..954
                     /locus_tag="VYQ30_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1967009.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1215..1538
                     /gene="tnpB"
                     /locus_tag="VYQ30_001960"
                     /pseudo
     CDS             1215..1538
                     /gene="tnpB"
                     /locus_tag="VYQ30_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          552 a          317 c          293 g          463 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaagcg aatacagact atattgctcg ctatggcgta catgcagtta tccctaatcc
      121 tgaacaaaaa caaattgttc ttgttcaaga accaaatggt gcttggttcc taccatgtgg
      181 agaaattgaa gcaggtgaaa atcatcagga agccctaaag cgtgagttga ttgaagagct
      241 tggtttcaca gcagaaattg gtacctatta cggacaagct gacgaatatt tctattctcg
      301 tcatcgtgat acctactact ccaatcctgc ctacctctat gaagcaactc ctttcaaaga
      361 agtacaaaag ccactagaaa actttaatca tattgcctgg ttccctattg acgaggctat
      421 caaaaacctt aaacgtggta gccataaatg ggccattgaa tcttggcaaa aacagcataa
      481 gattggctaa tactcttcga aaataaaatt caaaccacgt cagcgtcgcc ttaccgtact
      541 caagtatagc ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaatca
      601 agcgacttta ttcaaaaagt gctataatag aattagaaaa tcggaggaaa tattatgaag
      661 cttatcaata cgacaaactc acactcgcaa cttgtaaaaa gtcagctaga aagtaccgat
      721 gcaacccttg ttgaggttta ttctgcaggg aatacagatg ttatttttac ccaagctccg
      781 cttcactatg aaatcctcat ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa
      841 accattcaag aatttttctt gaaacgtaaa attgataaga cctctgttga tgaagccaat
      901 atcaagacac tctactcaga gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat
      961 tgagtaaaaa ctcgatttga gagaaagtcc aataagtctt tccataacaa aacgcatagt
     1021 aacaagagtt tctacaccca ctattatgcg tttttatcat tttgaaatct actgctaatc
     1081 aatgaagaag caaagatcaa actaggcagc tagttacaag ttacgtaagc gcgtcataac
     1141 aaggtatcta tcctaattcc ccaacttcca gtatactaga taaaaaataa ttatcggagg
     1201 aaaggtattg tactatgata attcaactaa gtgatttagg tcaagttcac cttgtttgtg
     1261 gtaagacaga tatgaggtag ggaatagact cattagccta tgtagttaaa acccactttg
     1321 aattggattc tttctccggt caagtctttc tcttttgtgg tggacgtaaa gaccgcttta
     1381 aagcccttta ctgggatggt caaggatttt ggctactata taaacgcttt gagaacggaa
     1441 aactgacttg gctaagtaca gaaaaggatg tcaaagctct cgcacctgaa caagtagatt
     1501 ggctgatgaa gggcttttct atcactccaa aaatatagta gattgaaact agaatagtac
     1561 acctctgctt ctaaaatatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt
     1621 tcttg
//
LOCUS       DAVMEF010000232         1616 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVMEF010000232 DAVMEF010000000
VERSION     DAVMEF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.233"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..486)
                     /locus_tag="VYQ30_001961"
     CDS             complement(<1..486)
                     /locus_tag="VYQ30_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1967010.1"
                     /translation="MTKLWKRYKPFVSAGIQELITYRVNFFLYRIGDVMGAFVAFYLW
                     KAVFDSSHQSLIQGFTLSDMTLYIIMSFVTNLLTKSDSSFMIGWEVKDGSIIMRLLRP
                     VHFAMSYLFTEIGSRWLVFVSVGLPFVILIAGLKLLSGESFLQIVLITTVYLLSLILA
                     FL"
     gene            complement(488..1480)
                     /locus_tag="VYQ30_001962"
                     /pseudo
     CDS             complement(488..1480)
                     /locus_tag="VYQ30_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          563 a          338 c          251 g          464 t
ORIGIN      
        1 caaaaaagct aagattaagc taagaaggta aactgtggtt attaaaacaa tttgtaagaa
       61 ggactcacct gataagagct ttagacctgc aatcagaatt acaaatggta gaccaacact
      121 aacaaaaact agccatctag acccaatttc tgtgaaaaga taagacattg caaaatgaac
      181 tggacgaagc agtcgcataa taatagagcc atcctttact tcccatccaa tcataaaaga
      241 actatctgat tttgtcagta gattcgtcac aaaactcatg atgatatata aggtcatatc
      301 agataaagta aaaccttgaa tgagagactg gtgagatgaa tcaaacacag ctttccacaa
      361 gtaaaatgct acaaaggccc ccatcacatc cccaatgcga taaagaaaaa aattgacacg
      421 atacgtgatt aattcttgga ttccagcgct aacaaaaggc ttatagcgtt tccataattt
      481 agtcatccta aagctccttt cgatagaagc gacgaataat atcttcaata ttcgcatccg
      541 tcatctttaa atcacggatc gtaaaatcag acaaagtttg ttgaataatg tcagccgttt
      601 gatactgcga gctgtcgtac tgaatatcaa gtgacaattc ctgtctcgtc acatagatat
      661 cagaaagtcc ttcaaaatga gagacaatat aatcctgccc aggatgtagt tcaaaagaga
      721 gagtcttcat cttcccgaaa gtctttttca gttgattcac agttccatca aagatttctc
      781 ggcccttatc aatcataaaa atacgatcac ataactgttc aatatcacct aaatcgtggg
      841 tcgttaaaag tatggtcgtc tcttcttctt gattaatctg tgtaatggca cgtcgaatat
      901 tatccttgac cgaaacatct aaaccaatag taggctcatc taggaaaaga actttcggat
      961 tatgcaataa ggaggccgca atatccgccc tcatacgttg acctagagat agagttctga
     1021 caggatcttg aataaattcc ttcaaatcta aaacttcatt tagaaaatcc atgcgttttt
     1081 gaaagaccgc atcaggaaca tcataaattt cctttaaaac cgagtaagtc tcctacaaag
     1141 ccaaatccca ccaaagttga gttcgctgcc caaaaactac accaatatct ttaacatagc
     1201 cttgacggtt ctcttgagga atctttccat tgatacgaca aaaacctgat gttggtttca
     1261 aaatccccgt caacatcttg atagttgttg atttccccgc tccatttgcg ccaataaagc
     1321 ctaaaatttg tcctttgggc acctcaaatg ataaatcttt aacagcttca aagacttgtc
     1381 tcttgggatg aaaaaaagag cgaaaagctc cctttaagcc aggttccttt attgttttca
     1441 caaaattttt ctgaagatgc tctactgtta tcatttttat aaatcactcc tttcgtttca
     1501 ctcaagttat aatatcaaca aaaattgtag catactacag taaagttaag aaaatttacc
     1561 atcattacaa accatatgaa aaaagctact aaaactatgc catcttgtat tttatc
//
LOCUS       DAVMEF010000233         1612 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVMEF010000233 DAVMEF010000000
VERSION     DAVMEF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.234"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            108..533
                     /locus_tag="VYQ30_001963"
     CDS             108..533
                     /locus_tag="VYQ30_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967011.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            941..1369
                     /locus_tag="VYQ30_001964"
                     /pseudo
     CDS             941..1369
                     /locus_tag="VYQ30_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          587 a          210 c          327 g          488 t
ORIGIN      
        1 cttttagaaa ataacagaat ttcgcaaggg aagtatgagg aactgttact agatgctttc
       61 agatatgata ttgtatatgg gctagatgaa gaggggggag ttgtcgtttg actagtcgtg
      121 tatttattga tgcagattgt atttcagtat ttttatgggt tggcactgaa catcttttag
      181 aaaagctcta tttgggtaaa attgttattc cacaagaggt gtatgatgaa atcaatatac
      241 ctacaattcc ccatttaaaa tctaggatag atcagttggt agctaagggt tcagctgaga
      301 ttgtgagcat agacattgga actgaagaat acgcattata tagagattta acaagaaatc
      361 atgatagtaa caagattatt ggtaagggag aaggggcatc tatttcctta gcgaaaaagc
      421 ataatgggat attaggaagt aataacctaa gagatgttaa atcatatgta gaagaatttt
      481 ctttagaata tatgacaaca ggagatatac tgattgaagc gtttaaagcg taatttatta
      541 ctgaataaga gggcaatcat atctggaata atatgcttaa aaagagaagg aaaattggtg
      601 caaattcatt ttcagactat cttcgtggaa gtattcatca aaatagacaa aaataaattt
      661 ggataaatcg aactcactat tcaggaggca tatgagcaat tcgaaaaaga aaagtgtcaa
      721 attgagccta taggagtaga agtgaaatag taagtcctgc atagtggatg agagaaaagt
      781 tctccttgaa gttttcctga actatcagtc gcatgtcaaa cgatatgtag ggtaatgtga
      841 gaggggatag cgagtagttt ttggttattt tatcaaaaaa cttatatttt attataccga
      901 atgataaaat ataataaaaa tgatagaata aggaaaaaac atgaatgtca aaaagataat
      961 gtcaattttt caatcctttt atgttgatgt cagtattgag gaactgactt tgactttacc
     1021 aatcagtttt gtaaaaaggt ttgagtatac tcaaatgact tttcataagg aatcattttt
     1081 attgattaaa gaaaagagaa gggggagttt gagttcattt gttactcagg ctcgcactat
     1141 gggtgaaaaa gccaatatgg atgttgtttt ggtgttttcg aagttatcag acagtgaaaa
     1201 aaagcaatta cttcaagcta gagttccgtt tgtagacttt aagggaaacc tcttcttccc
     1261 tccattggga ctagtactca atgcgaatga tactgaagtc cctaaggaat taacacctag
     1321 cgaacaatta acgtggattg cctttttatt gacaaaaggt caaaaagtag gctctataat
     1381 atttgtagtg ggtaaatcca ctatagatat tatggagcct attttattgt agaaaaaaag
     1441 tcccataaga tctataatga aaagcgatca aacaactcat tagaaagatt catatgggac
     1501 aattacattt tatcacaaaa ctactcgata tcaaagacac aaatatccaa attatagatg
     1561 tcgtcaatag ggattcacac aaagaaatca tcgccaaact ggactacgac gc
//
LOCUS       DAVMEF010000234         1609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVMEF010000234 DAVMEF010000000
VERSION     DAVMEF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.235"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            35..106
                     /locus_tag="VYQ30_001965"
     tRNA            35..106
                     /locus_tag="VYQ30_001965"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67..69,aa:Gln,seq:ttg)
     gene            268..1470
                     /gene="rpsA"
                     /locus_tag="VYQ30_001966"
     CDS             268..1470
                     /gene="rpsA"
                     /locus_tag="VYQ30_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1967012.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSIKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT          494 a          263 c          373 g          479 t
ORIGIN      
        1 gggggttcga atccctctct ctccatttca ttaatggggt atagccaagc ggtaaggcaa
       61 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagttct taggtaataa
      121 tcaagataaa aagcaaaata tcttagggta ttttattttt ataattgaaa gacgtgaatg
      181 atatgaacat gtccttgcgg gtgcttagga aaaaaattat aagtatgtca agtttaagaa
      241 aaacttgatt gttggaggat tttttagatg aacgaatttg aagatttgct aaatagcgtt
      301 agtcaagttg agactggtga tgttgttagt gctgaagtat tgacagttga tgcgactcaa
      361 gctaacgttg caatctctgg aactggtgtt gaaggtgtct tgactcttcg cgaattgaca
      421 aacgatcgtg atgcagatat caatgacttt gttaaagtag gagaagtatt ggatgttctt
      481 gtacttcgtc aagtagttgg taaagatact gatacagtta cataccttgt atctaaaaaa
      541 cgccttgaag ctcgcaaagc atgggacaaa cttgttggtc gcgaagaaga agttgttact
      601 gttaaaggaa cgcgtgccgt taaaggtgga ctttcagtag aatttgaagg tgttcgtgga
      661 tttatcccag cttcgatgtt ggatactcgt ttcgtacgta acgctgagcg ttttgtaggt
      721 caagaatttg atactaaaat caaagaagtt aacgctaaag aaaaccgctt catcctttca
      781 cgtcgtgaag ttgttgaagc agctactgca gcagctcgcg ctgaagtatt cggtaaattg
      841 gctgttggtg atgttgtaac tggtaaagtt gctcgtatca caagcttcgg cgctttcatc
      901 gaccttggtg gtgttgatgg attggttcac ttgactgaat tgtcacatga acgtaacgta
      961 tcaccaaaat cagttgtaac tgttggtgaa gaaattgaag tgaaaatcct tgatcttaac
     1021 gaagaagaag gacgtgtatc actttctatt aaagcaacag taccaggacc atgggatggc
     1081 gttgagcaaa aattggctaa aggtgatgta gtagaaggaa cagttaaacg tttgactgac
     1141 ttcggtgcat ttgttgaagt attgccaggt atcgatggac ttgttcacgt atcacaaatt
     1201 tcacacaaac ggattgaaaa tccaaaagaa gctcttaaag ttggtcaaga agttcaagtt
     1261 aaagttcttg aagttaacgc agatgcagaa cgcgtgtcac tttctattaa agctcttgaa
     1321 gaacgtccag cccaagaaga aggacaaaaa gaagaaaaac gtgttgctcg tccacgtcgt
     1381 ccaagacgtc aagaaaagcg tgatttcgaa cttccagaaa cacaaacagg attttcaatg
     1441 gctgatttgt ttggtgatat cgaactttaa tcaaattgaa aattcacaaa atcctttgtt
     1501 tactaaacaa aggatttttc tggctctttg tcaactgtag tgggttgaaa aaaagctaag
     1561 ctcgagaaag gacaaatttc gtcctttctt ttttgatatt cagagcgat
//
LOCUS       DAVMEF010000235         1594 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVMEF010000235 DAVMEF010000000
VERSION     DAVMEF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.236"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..769
                     /locus_tag="VYQ30_001967"
     CDS             <1..769
                     /locus_tag="VYQ30_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1967013.1"
                     /translation="ELEILDYIDTLCKKHNINYIINYGTLIGAVRHEGFIPWDDDIDL
                     SMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKIIDTRNTKTYESG
                     IFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKDWIRTAFWLLLRP
                     VSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKTIDLPFENLSLPA
                     PEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            771..1580
                     /locus_tag="VYQ30_001968"
     CDS             771..1580
                     /locus_tag="VYQ30_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1967014.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          530 a          287 c          284 g          493 t
ORIGIN      
        1 tgaattagaa attttggatt atattgatac tctctgtaaa aagcacaata tcaactatat
       61 tattaactac ggtactctga ttggggcggt tcgacatgag ggctttatcc cttgggacga
      121 cgatattgat ttatccatgc ctagagaaga ctaccaacga tttattaaca tttttcaaaa
      181 ggaaaaaagc aagtataagc tcctatcctt agaaactgat aagaactact ttaacaactt
      241 tatcaagata accgacagta cgactaaaat tattgatact cgaaatacaa aaacctatga
      301 gtctggtatc tttatcgata ttttccctat agatcgcttt gatgatccta aggtcattga
      361 tacttgttat aaactggaaa gcttcaaact gctgtctttc agtaaacata aaaatattgt
      421 ctataaggat agccttttaa aagattggat acgaacagcc ttttggttgc tccttcgacc
      481 ggtttctcct cgttattttg caaataaaat cgagaaagaa attcaaaaat atagtcgtga
      541 aaatgggcag tatatggctt ttattccttc aaaatttaag gaaaaggaag tcttcccaag
      601 tggtaccttt gataaaacaa tcgatttacc ctttgagaat ttaagccttc ctgcacctga
      661 aaaatttgat actattttga cacaatttta tggagattat atgaccctac caccagaaga
      721 aaaacgcttc tacagtcatg aatttcacgc ttataaattg gaggattagg atgcaatatt
      781 tagaaaaaaa agaaattaaa gaaattcaac tagccctgct ggactatatt gatgagactt
      841 gtaagaaaca tgatattcct tattttctca gttatggaac catgcttgga gccatccgcc
      901 acaaaggtat gattccttgg gatgatgata ttgatatttc cctttatcgt gaggattatg
      961 agcgtttact gaagattatt gaagaagaaa atcaccctcg ctacaaggtt ctttcctacg
     1021 atacatcttc ttggtacttc cataatttcg catcgatttt ggacacttct actgttatag
     1081 aagaccatgt taagtacaag cgtcatgata ccagcctttt catcgatgtc ttcccaattg
     1141 atcgatttac agacttgagc attgtcgaca agagctataa gtatgtggcc cttcgtcaac
     1201 tagcttatat caaaaaatca cgagcagttc acggtgatag caaactaaaa gattttctta
     1261 gattatgtag ctggtacgct ctccgatttg tcaatcctcg ctacttttac aagaaaattg
     1321 atcaactagt caaaaatgct gtaaccaaca ctcctcaata tgaaggagga gttgggatcg
     1381 gtaaggaagg gatgaaagaa atcttcccag ttgatacctt taaagaactg attttaactg
     1441 agtttgaggg ccgtatgttg cctgttccca aaaaatatga ccaattttta acccagatgt
     1501 atggcgatta tatgacacca ccatcaaaag aaatgcaaga gtggtatagt catagcatta
     1561 aagcttatcg caaaaactga ttgaggggga ttat
//
LOCUS       DAVMEF010000236         1567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVMEF010000236 DAVMEF010000000
VERSION     DAVMEF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.237"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            5..652
                     /locus_tag="VYQ30_001969"
     CDS             5..652
                     /locus_tag="VYQ30_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1967015.1"
                     /translation="MVSCQALEGEPLYTPNGGVMPLLAKAAFQAGAKGIRANSVRDIS
                     EIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPGYDNI
                     TDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYTPYSPKKDNPDFEL
                     VERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV"
     gene            653..>1567
                     /locus_tag="VYQ30_001970"
     CDS             653..>1567
                     /locus_tag="VYQ30_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1967016.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLG"
BASE COUNT          492 a          221 c          310 g          544 t
ORIGIN      
        1 aataattgtt tcctgccaag cgttagaagg agagccactt tatactccaa atggaggagt
       61 gatgccgtta ttagcaaaag ccgcctttca agcaggcgct aaaggtattc gtgctaattc
      121 tgtccgcgat atttcagaaa taaaagaaga agttgactta ccaattatag gtatcataaa
      181 aagagattac gatggctttg aaccttttat ttcagcaact atgaaagaga ttgatgaatt
      241 agttagtgaa ggagtagaca ttttagcttt ggattgtact aatcgttcaa gaccaggtta
      301 tgataatatt actgacttta ttcatgatat taaagtcaag tatccaaacc aattattaat
      361 ggctgatatt tctacttttg aagaagggaa agtagctgct gaatcgggag tagattttgt
      421 gggtacgaca ttatctggtt atacaccata tagccctaaa aaagataatc ctgattttga
      481 gttagttgaa agattggtaa aagagcttga cgtaccagtt attgctgaag gaagaataag
      541 tactccggaa caagctagaa aaatgttaga tttaggtgct tacgcagtgg ttgttggagg
      601 agctatcact cgtcctttgg aaatagcgaa gaagtttatt gaggtagttt agatgaatca
      661 agaaatatta aaaaagagta agattcaatt gacagttgaa catattcaaa aatattggat
      721 tttatattta atgatgattc cagcaactgt tctactaatt ttatttacgt atggaccaat
      781 gtacggcatt attatggcct ttcaagactt tactgtcttc aagggatata ctggaagtcc
      841 atttgtcggt ttaaaacatt ttcaaagatt gttctcagat cctttgtttt atagattgtt
      901 caagaatacg ttcatggtag gggttttaga ctttttattc tctttcccag ctccgcttat
      961 ttttgcactc atattaaacg aagtcagaaa agtaagattt aagagtgtag ttcaatctat
     1021 tagttatctt cctcatttta ttcccctagt ggttatggtt ggtattattt ttgaactatt
     1081 tggttcatat ggtattatta acagtttact ttcatcactt ggaatggagc caataagttt
     1141 ctttacaaaa tctgaatggt tcttgcctct ctacattggt tcaggagtat ggaaaacaat
     1201 aggttggggt tctatcattt atatgggagc attaaccaat atagattcaa ctttatatga
     1261 agcagcagat atggacgggg ctaatagatg gcacaaaatg tggcatgtaa ctctaccaag
     1321 tttaagacct acagtagtta ctttgtttat actaaatgct ggtggtatca tgcaagtagg
     1381 atttgaaaag gtattcttga tgtctagtcc tgcaacatat gaagtatctg atgtattatc
     1441 tacttatgtt tatagacaag gtattttaaa ttctgatttc agttactcag ctgcagtagg
     1501 attatttaat aacatagtag ctttgttatt tgttttatta gctaataaga ttgctaaaaa
     1561 acttggc
//
LOCUS       DAVMEF010000237         1562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVMEF010000237 DAVMEF010000000
VERSION     DAVMEF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.238"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1562
                     /locus_tag="VYQ30_001971"
     CDS             <1..>1562
                     /locus_tag="VYQ30_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1967017.1"
                     /translation="QVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDK
                     TAMWALSGNFDASKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQIN
                     ISGDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNI
                     AYAKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLV
                     KIYNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLL
                     TSGVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDG
                     NKDGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSS
                     SGEEVFRRHFDKVVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKL
                     SNIALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEEL
                     EKLIIYNRTDAE"
BASE COUNT          568 a          216 c          319 g          459 t
ORIGIN      
        1 caagtaatgt tgcctatgta tactaataat acaaagtcgt tatcgttaga tttgacgcca
       61 catacttacg ctgttgttaa agaaaatcca aataatttac atattttatt gaataattac
      121 aggacagata agacagctat gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg
      181 aagaaagaag aattagagtt agcgaactgg ataagcaaaa attattccat caatcctgta
      241 gataatgact ttaggacaac aacacttaca ttaaaagggc atactggtca taaacctcag
      301 ataaatataa gtggcgataa aaatcattat acttatacag aaaattggga tgagaatacc
      361 catgtttata ccattacggt taatcataat ggaatggtag agatgtctat aaatactgag
      421 gggacaggtc cagtctcttt cccaacacca gataaattta atgatggtaa tttgaatata
      481 gcatatgcaa aaccaacaac acaaagttct gtagattaca atggagaccc taatagagct
      541 gtggatggta acagaaatgg taattttaac tctggttcgg taacacacac tagggcagat
      601 aatccctctt ggtgggaagt cgatttgaaa aaaatggata aagttgggct tgttaaaatt
      661 tataatcgca cagatgctga gactcaacgt ctatctaatt ttgatgtgat tctatatgac
      721 aataatagaa acgaagttgc taagaaacat gttaataatt tgtcgggtga atctgttagt
      781 ctagatttca aagaaaaagg agcaaggtat attaaagtta aattactaac gagtggagtg
      841 cctttgagtt tagcagaagt agaggttttt agagaatcag atggtaagca atctgaagag
      901 gatatagata aaataacaga agataaagta gtctctacaa ataaggtagc tactcaaagt
      961 tcaaccaatt atgagggtgt agctgcttta gcagttgatg gtaataaaga tggagattac
     1021 ggacatcatt cggtgactca tactaaggca gattctaacg cttggtggca ggtcgatctg
     1081 ggagaagagt ttacggtttc taaagttgat atttataata gaacagatgc cgaacctcag
     1141 cgtttatcta attttgatgt tatttttcta tcttcatcag gagaagaagt ttttagaaga
     1201 cattttgata aagtagttga tggtttgtta tctttaaaag taccttctgt aggggctaag
     1261 ctagtcaaaa tagaattaaa atcagcagct attccgttaa gtttagcgga agttgaagtc
     1321 tatggttcaa agagaactcc gaagaaactt tctaatattg cattaacaaa agaaactcga
     1381 cagagttcaa cggattacaa tggtttttct cgtctagcag ttgatggaaa taaaaacgga
     1441 gattatggtc atcattcagt gactcatacc aaagaagatt ctccttcatg gtgggagata
     1501 gatttagcac aaaccgaaga attagaaaag ttaattattt ataatagaac agatgctgaa
     1561 at
//
LOCUS       DAVMEF010000238         1547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVMEF010000238 DAVMEF010000000
VERSION     DAVMEF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.239"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(179..1525)
                     /gene="celB"
                     /locus_tag="VYQ30_001972"
     CDS             complement(179..1525)
                     /gene="celB"
                     /locus_tag="VYQ30_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1967018.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
BASE COUNT          562 a          320 c          248 g          417 t
ORIGIN      
        1 acagcagttt cctgtttatc aactattcga actactttct attttgcttc ataccctaca
       61 tagcgaaaaa atatgaaaaa gcagagaaga atatcttaaa aagacctctt cactgctaat
      121 attaacactc attatttaaa ctatatggat tctatcatcg agtatacttt tttacttatt
      181 agataccttg ctcttctttc accaattttt gatcatatac acggatgaat ggaagataga
      241 ctaagaatgc tgcaaatgca catactagag caactaatac agctcgaaga tctgctgtcc
      301 ctaagaaagc tccaatccct actggagttg gccatggaac ctgtgcgata attggcttaa
      361 taaagtttag agaattcgct acgtaataaa tagtagcagt aaccattggt gctaaaataa
      421 atggtatagc caaggctgga ttatagataa taggtaatcc aaaaattaat ggttcattaa
      481 tattaaataa ggctggaact acagatgctc gtcctattgc tttaagctgt tcagatttag
      541 aggcaaaagc aatatataaa catagtccta aagttgcacc agaaccacct gcaattacaa
      601 acatattaga aaattcacct gcaacagcga agtgcccgcc agcagcattt tcagccatgt
      661 tagcaagagc aattggacta acaaatgcaa aaacaatgtt cgcaccgtgg atacctacaa
      721 tccaaagtag ttgagtcaat agataaataa tcattaaacc aatccacgaa ttagtcagat
      781 tggatacaaa accaaatgga attgcaatga ctttaaaaat atctgttccc attgctacaa
      841 gaagaccgtt gataaagata acaacaaatg caacaacaaa tcccggaacc aaagcggtaa
      901 atccacgaga aactccttct ggaacagctt caggcatttt aataacccaa ttatgtttaa
      961 cacacatacg ataaataaga acagtcacaa ttgccataat gattgcggta aaaatccctg
     1021 ttgtcccaaa acgtgcgact ccatttccca ttgcccatcc atctgcaatt actgcacctt
     1081 cttttagact tgtcacagtc ttcatcattc caccatcaaa aatgatttgc ggtactgtca
     1141 tgacaaaagc catcaaggca agcaaggcac cattaagagg attcatattg agttcttctt
     1201 cctctgcata aatttttgtc aattcatatg caagtgatag aacgaaataa agagatagag
     1261 aacccatagt cgcatagttt gcaaccatgt aaagtgatgt gaatttatca aatgaagcag
     1321 agaaaatatc tgccacaatt ggccaaaatg agaaagcttg tggcaaaata ctgaatacca
     1381 aaaacattga tcctacaata gtaaatggta cagcagccat acctgcagcc gtgatagcac
     1441 gtactacttt aaactgagca agtttgccca ttggtcccat aacatggttt tcaagaaaac
     1501 caaacaaccc gttttgttga tccataaata gacctcctta ataaaac
//
LOCUS       DAVMEF010000239         1522 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVMEF010000239 DAVMEF010000000
VERSION     DAVMEF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.240"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1350
                     /locus_tag="VYQ30_001973"
     rRNA            <1..1350
                     /locus_tag="VYQ30_001973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1427..>1522
                     /gene="rrf"
                     /locus_tag="VYQ30_001974"
     rRNA            1427..>1522
                     /gene="rrf"
                     /locus_tag="VYQ30_001974"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          413 a          325 c          444 g          340 t
ORIGIN      
        1 ggggagcgaa gtttagtagc gaagttagtg acgtcacact gccaagaaaa gcttctagcg
       61 tttaaacata ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag
      121 gtgagcgaga gaactctcgt taaggaactc ggcaaaatga ccccgtaact tcgggagaag
      181 gggtgctgac tttaagtcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac
      241 agctctctgc taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg
      301 ttaagaggag tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg
      361 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
      421 aggcgtaatg atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga
      481 agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata
      541 ttgagtgtct gtaccacatg tacaggatag gtaggagtct aagagatcgg gacgccagtt
      601 tcgaaggaga cgctgttggg atactaccct tgtgttatgg ccactctaac ccagataggt
      661 gatccctatc ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag
      721 gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgca gagtgtaaag
      781 gtataaggga gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt
      841 gatccggtgg ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata
      901 acaggcttat ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc
      961 tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg
     1021 cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag
     1081 gaaatttgag aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta
     1141 ccagttgtct tgccaaaggc atcgctgggt agctatgtag ggaagggata aacgctgaaa
     1201 gcatctaagt gtgaaaccca cctcaagatg agatttccca tgattatata tcagtaagag
     1261 ccctgagaga tgatcaggta gataggttag aagtggaagt gtggcgacac atgtagcgga
     1321 ctaatactaa tagctcgagg acttatccaa agtaactgag aatatgaaag cgaacggttt
     1381 tcttgaattg aatagatatt caattttgag taggtattac tcagagttaa gtgacgatag
     1441 cctaggagat acacctgtac ccatgccgaa cacagaagtt aagccctaga acgccggaag
     1501 tagttggggg ttgccccctg tg
//
LOCUS       DAVMEF010000240         1517 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVMEF010000240 DAVMEF010000000
VERSION     DAVMEF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.241"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1517
                     /locus_tag="VYQ30_001975"
     CDS             <1..>1517
                     /locus_tag="VYQ30_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1967019.1"
                     /translation="KFREVEDFVTGGKRTVELDYKYDDKGNITAYEDESALEYETEKF
                     DEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIKATKYDSHSKTMT
                     FDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKETKAEYPYVSSYGNV
                     IELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKTD
                     MLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFNII
                     RYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLF
                     IDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYLI
                     YGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNA
                     KAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQ"
BASE COUNT          604 a          185 c          296 g          432 t
ORIGIN      
        1 gaaatttaga gaagttgagg actttgtaac tggtggaaaa agaacagtag aacttgatta
       61 taaatatgac gataaaggaa atataactgc ctatgaagat gaaagtgctc tagaatatga
      121 aactgaaaaa tttgacgaaa taaaatcaaa aatttatggt gttctaagcc catctaaaga
      181 tggacacttt gaaattcttg gaaagataag caatgtttct aaaaatgcca aggtatatta
      241 tggaaatagc tataaatcga tagaaatcaa agcgactaag tatgattccc attcaaaaac
      301 aatgacattt gatttatacg ctaatattaa tgatattgtg gatggattag cttttgctgg
      361 agatatgcgg ttttttgtta aagatgataa tcgaataaaa gctgaaacta aaattagaat
      421 gcctgaaaaa aataaggaaa ctaaagcaga atatccctat gtatcaagtt atgggaatgt
      481 aatcgaatta ggagaaggag atctttcaaa gaacaaacca gacaatttaa ctaaaatgga
      541 atctggtaaa atctattctg attcagaaaa acaacaatat ctgttaaagg ataacatcat
      601 tctaagaaaa ggctatgcac taaaagtgac tacctataat cctggaaaaa cggatatgtt
      661 agaaggaaat ggagtctata gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa
      721 tctaagagtc ctttcagaaa caacaattta tgctgatagt agaaatgttg aagatggaag
      781 aagtacccaa gctgtattaa tgtcggcttt ggacggcttt aacattataa ggtatcaagt
      841 gtttacattt aaaatgaacg ataaagggga agctatcgat aaagacggaa atcttgtgac
      901 agattcttct aaacttgtat tatttggtaa ggatgataaa gaatacactg gagaggataa
      961 gtccaatgta gaagctataa aagaagatgg ctccatgtta tttattgata ccaaaccagt
     1021 aaacctttca atggataaga actactttaa tccatctaaa tctaataaaa tttatgtacg
     1081 aaatccagaa ttttatttaa gaggtaagat ttctgataag ggtggtttta actgggaatt
     1141 gagagttaat gaatcggttg tagataatta tttaatctac ggagatttac acattgataa
     1201 cactagagat tttaatatta agttgaatgt taaagacggt gacatcatgg actggggaat
     1261 gaaagactat aaagcaaacg gatttccaga taaggtaaca gatatggatg gaaatgttta
     1321 tcttcaaact ggctatagcg atttgaatgc taaagcagtt ggagtccact atcagttttt
     1381 atatgataat gttaaacccg aagtaaacat tgatcctaag ggaaatacta gtatcgaata
     1441 tgctgatgga aaatctgtag tctttaacat caatgataaa agaaataatg gattcgatgg
     1501 tgagattcaa gaacaac
//
LOCUS       DAVMEF010000241         1516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVMEF010000241 DAVMEF010000000
VERSION     DAVMEF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.242"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..1370
                     /gene="gdhA"
                     /locus_tag="VYQ30_001976"
     CDS             24..1370
                     /gene="gdhA"
                     /locus_tag="VYQ30_001976"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1967020.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          454 a          296 c          327 g          439 t
ORIGIN      
        1 tatgacaata aaaggagaac gatatgacat ctgctaaaga atatatccaa agcgtgtttg
       61 aaactgtgaa agctcgtaac gggcacgagg ctgaattcct ccaagctgtt gaagaatttt
      121 tcaacacttt ggaacctgta tttgaaaaac accctgagta tatcgaagaa aatatcttgg
      181 cacgtattac tgagcctgag cgcgtggttt ctttccgtgt tccttgggtt gaccgtgatg
      241 gaaaaattca agtaaaccgt ggttaccgtg ttcaattcaa ctcagctgtt ggaccataca
      301 aaggtggact tcgtttccac ccaactgtaa accaagggat cttgaaattc ctcggatttg
      361 aacaaatctt taaaaatgtc ttgactggac ttcctatcgg tggaggtaaa ggtggatcag
      421 acttcgatcc taaaggtaaa acagatgctg aagtgatgcg cttctgccaa agcttcatga
      481 ctgaattgca aaaacacatc ggaccatcac ttgacgtacc tgctggtgat atcggtgttg
      541 gtggacgtga aattggttac ctttacggtc aatacaaacg tcttaaccaa tttgatgctg
      601 gtgtcttgac tggtaaacct cttggatttg gtggtagctt gattcgtcca gaagcaactg
      661 gttacggttt ggtttactat actgaagaaa tgctcaaagc taacggtaac agctttgctg
      721 gtaagaaagt cgttatttca ggttctggta acgttgctca atatgctctt caaaaagcaa
      781 ctgaactcgg tgcaactgtt atttctgttt ctgactcaaa tggttatgtc atcgatgaaa
      841 atggtatcga cttcgatctt ttggttgatg ttaaagaaaa acgtcgtgct cgtttgactg
      901 agtatgcagc tgagaaagca actgctactt atcatgaagg ttctgtatgg acttacgctg
      961 gaaactatga cattgcgctt ccatgtgcta ctcaaaacga aatcaacggt gaagcagcta
     1021 aacgtttggt tgctcaaggc gttatctgtg tatctgaagg tgccaacatg ccaagcaacc
     1081 ttgatgccat caaagtttac aaagaaaatg gtatcttcta cggacctgcc aaagctgcca
     1141 acgctggtgg tgtagccgtt tcagcccttg aaatgagcca aaacagcctt cgcctctcat
     1201 ggactcgtga agaagttgat ggccgtctca aagacatcat gacaaacatc ttcaacacag
     1261 ctaaaacgac ttcagaaaca tacggtcttg ataaagacta ccttgcagga gctaacattg
     1321 ctgcctttga aaatgtagca aacgctatga ttgcccaagg tattgtttaa gatttatttg
     1381 cttaatcctc aatcactacg attggggatt tttagttgag ttagacagtt gggaaattct
     1441 aattaaatga aataaaaact gaacaaattg attgcggaat tcaaaccaat ttctaacata
     1501 cttctagaaa ttaatt
//
LOCUS       DAVMEF010000242         1513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVMEF010000242 DAVMEF010000000
VERSION     DAVMEF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.243"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(121..689)
                     /locus_tag="VYQ30_001977"
                     /pseudo
     CDS             complement(121..689)
                     /locus_tag="VYQ30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(765..977)
                     /locus_tag="VYQ30_001978"
     CDS             complement(765..977)
                     /locus_tag="VYQ30_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1967021.1"
                     /translation="MIQPLLHADETSYRVLESDSQLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVPEFLGDYSGYVHCDMLRQ"
     gene            complement(986..1291)
                     /locus_tag="VYQ30_001979"
     CDS             complement(986..1291)
                     /locus_tag="VYQ30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV1967022.1"
                     /translation="MFPVEREEITYKCKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1278..1472)
                     /locus_tag="VYQ30_001980"
     CDS             complement(1278..1472)
                     /locus_tag="VYQ30_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1967023.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          424 a          331 c          276 g          482 t
ORIGIN      
        1 atatttctca ctatttataa tggattgaaa taagatatga acaaatcgat taggaaagga
       61 aaattgattt atagaaatgt tttagcagtc gatgcgtact gttatagatt caacgcacta
      121 taattgatga cgtttagctg tttccaacaa actcataata atagctcttg ctttagctcc
      181 ttcaaaactt tgagaaaata accaccctcg cttttttaaa ctgagcttag gctaaaagag
      241 tccactggac tcttttactc cgtcccataa ccaatgattt aatggcgcat tcagctagat
      301 tattggaaag gaccagatgt ccgtctttca aaatagtctt aaaggtttct ttatacttga
      361 ggctatactt gaggctgtat tcaattgccc ttcctagttt tgaacccgat aaaactgact
      421 gacgccggca ccaagcaaag aaatcttcca ttaggggttg gagctcttct tgacgtttct
      481 gtagtcgttc atcagctggc aaagcctccc agtctctctc tttccaagga aaataactga
      541 tcacaataag ctaaaccttt agctcctaag gatgatttat ctgcttgctt gggggtcgct
      601 tcaaaaaact tccttctcac atgcgcccaa catccaacaa gttcagcttc ttccagttga
      661 cgataagctt ctagcggttc gcagtttacc aagcttagcg tcgccttgct cctaagcctt
      721 ggactgctat cgcataggca gaactagagg actaaagtcc taagttactg ccgcaacata
      781 tcacaatgca catagccaga ataatctcct aggaattctg gcactaccga accactccga
      841 cgctgatcat ggtggtacag cgtgattgct tgattctcag ctttcccaga caaaaaggtc
      901 caatagtagg tcagctgact atcactctct agcaccctat aagaagtttc atctgcatga
      961 agtaagggct gaatcaatag tctcgttata aaggggcttt aggaatagga gcttttacaa
     1021 ttttatcact tggattattc ttactgcatg tttggcattt gtaagcgtgt tggatatgat
     1081 ccactctttt taattgcgca ggaataaaga ccaattcttg tcgttgaagg ctcgctccaa
     1141 tctcttttaa atcgtcctga caatcagggc aaatgctctc ttctacttga tgatgaactt
     1201 cttctgaatc aaattgggca agaatagctt gacgtttccc tttagatttc ttacatttat
     1261 aggtgatttc ttctctttca actgggaaca tctccatctt tctcagatgg agactcttcc
     1321 tcaaaaaggt tgagttgtcc aaaagggcaa acacttttct cagaggattt tccagagagc
     1381 ttttgcgtta gataagccac ttgttcacac agaagggaaa gttcattagt gagactatca
     1441 attatagcac tctgttgttg aatgattttc aatcttctcc catagacttt cctccttatt
     1501 tcctaacttt att
//
LOCUS       DAVMEF010000243         1508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVMEF010000243 DAVMEF010000000
VERSION     DAVMEF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.244"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1508
                     /locus_tag="VYQ30_001981"
     CDS             <1..>1508
                     /locus_tag="VYQ30_001981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967024.1"
                     /translation="LAEAEALVLAEADALVLAEADALVEAEADALVDAEAEALVDADS
                     DAEVLATDDLETSVPVFGNCCLDLPAEAEALVDAEAEALVDADSEALVDAEAEALVDA
                     DSEALVDAEAEALVDADSEALVDAEAEALVEAEADVDAEADALVLAEAEALVEADSEA
                     EVLAEADALVEADSDAEVLAEAEALVDADSEADVLAEAEALVLAETDALVLAEAEALV
                     DAEAEADVDAEADALVLAEADALVLAEAEALVEADSDAEILAEADALVDAEADALVDA
                     EAEALVLAEADALVLAEADALVDAEAEALVEADADALVLAEADALVDADSDAEVLAEA
                     EALVDAEAEALVEAEADALVDAEAEALVDAEAEALVDAEAEALVLAEAEALVDADSDA
                     EVLAEADALVDADSEALVLAEDDALVLAEAEALVLAEAEALVLAEDDALVEAEADALV
                     DAEAEALVDAEAEALVDAEAEADVDADSDAEVLAEADALVDADSEADVLAEA"
BASE COUNT          310 a          335 c          488 g          375 t
ORIGIN      
        1 cttgctgaag ctgaagcact ggtacttgct gaagctgacg cactggtact tgctgaagcc
       61 gacgcactcg ttgaggctga ggctgacgca ctcgttgatg ctgaagctga ggcgcttgtc
      121 gatgcagatt cagacgctga ggtgcttgct acagatgact tggaaacttc tgtacctgta
      181 tttggcaatt gctgtcttga cttacctgct gaagctgaag cgcttgtcga tgctgaggct
      241 gaagcactgg ttgatgccga ttcagaggca cttgttgacg ctgaggctga agcactggtt
      301 gatgccgatt cagaggcact tgttgatgct gaggctgaag cactggttga tgccgattca
      361 gaggcactcg ttgacgctga ggctgaggcg ctggttgaag ctgaggcaga cgttgatgct
      421 gaagctgacg cacttgtact tgctgaagcc gaagcactcg ttgaggccga ttcagaagct
      481 gaggtacttg ctgaagccga cgcacttgtt gaggccgatt cagacgctga ggtgcttgct
      541 gaagccgaag cactcgttga tgcggattca gaagctgatg tacttgctga ggctgaggca
      601 ctggtgcttg ctgagactga tgcactggta cttgctgagg ccgaagcact cgttgatgct
      661 gaggctgagg cagacgttga tgctgaagct gacgcacttg tacttgctga agctgacgca
      721 cttgtacttg ctgaagccga agcactcgtt gaggccgatt cagacgctga gatacttgct
      781 gaagctgacg cactcgttga tgctgaagct gacgcactcg ttgatgctga agctgaggcg
      841 ctggtacttg ctgaagctga tgcacttgtg cttgctgaag ctgatgcact tgttgacgct
      901 gaagccgaag cactggttga ggctgatgct gacgcacttg tgcttgctga agctgatgca
      961 ctcgttgatg cagattcaga cgctgaggtg cttgctgagg ctgaagcact tgttgacgct
     1021 gaagccgaag cactcgttga ggccgaagct gacgcactcg ttgatgctga agccgaggca
     1081 ctggttgacg ctgaagctga ggcgcttgtt gatgctgagg ctgaggcact agtacttgct
     1141 gaagccgaag cactcgttga tgccgattca gacgctgagg tacttgctga agccgatgca
     1201 cttgttgatg cggattcaga ggcactggta cttgctgagg atgacgcact ggtacttgct
     1261 gaagctgagg cgcttgtact tgctgaagcc gaggcgctgg tgcttgctga ggatgacgca
     1321 ctcgttgagg ccgaagctga cgcactcgtt gatgctgaag ccgaggcact ggttgacgct
     1381 gaagctgagg cgcttgttga tgctgaggct gaggcagacg ttgatgcaga ttcagacgct
     1441 gaggtgcttg ctgaagccga tgcactcgtt gatgcggatt cagaagctga tgtgcttgct
     1501 gaagctga
//
LOCUS       DAVMEF010000244         1507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVMEF010000244 DAVMEF010000000
VERSION     DAVMEF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.245"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            64..348
                     /locus_tag="VYQ30_001982"
     CDS             64..348
                     /locus_tag="VYQ30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967025.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            341..898
                     /locus_tag="VYQ30_001983"
     CDS             341..898
                     /locus_tag="VYQ30_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HEV1967026.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT          549 a          248 c          247 g          463 t
ORIGIN      
        1 gaaaaactac atttttgaat ataaactaca ataatataaa ctaaatttta taggaggaag
       61 acaatggatt ggtacgatta tatgatacag gcatccaaac aatcacaatt caacgcaagc
      121 cattggtttc gctatttgcg aaaagttatt tttgaagact attcttattt aacaaaccaa
      181 gatgtagaaa agttgctaga ctccaaagaa ctaacccgtt ttcaaaaaat tagcttgaag
      241 tatgcctttc aagagcatac tccaactcat aaatatgtga tttcattaaa taaacctgct
      301 aagttaacca atgttcaaaa attgatggag aaatacaaac atggataaaa tgaaaccggt
      361 cttccaagcc ctaaataagg aattaattca ggaaaatctg actttaacaa ttatctgtgt
      421 cggtggttat gtcttagaat atcatggttt acgtgccaca caagatgttg atgcttttta
      481 tgaccaaaat cagaaaatca atgaaattat tgctcgtgta ggaaaacagt ttaatcttaa
      541 cacccacgaa gagttatggc tcaacaatca tgttgcaaat atgaacaaac aacctccctt
      601 aagtttatgc gaatccttgt actcatttga gaatctgacc gttttagtag tccctattga
      661 gtatgtgtta ggaatgaaga tgatgagtat cagagagcag gatttgaaag atattggtgc
      721 aattattaaa tacaaaaact ttcattcacc attcgatacc tttaaatatt taaaagacat
      781 gggatttgat actattgacc tctctgttct attagaaggt tttagttatg cttatggtat
      841 ggattggtta gaaaaatttt ttaaagaaaa tcaagataaa cttcgcgaat tttattaaca
      901 gtatcaaaca agatgattgt caattagcca gtcgtcttgt ttttatgatg aaatgaaata
      961 aaatcggaac aaatcactta gagaattcca gtcaatttct agcactgttt gagacgtttc
     1021 agcggactat agcgaaatga aataagaata ggacaaatcg atcaggacag tcaaatcgat
     1081 ttctaagaat gttttagaag taaaggtgta ctattctact ttcaatctat tatatttcag
     1141 attcggtcca ctattccttc ccaaaaacat tgcatattgc tccgaaggaa ttgctcatac
     1201 tatcccaagc acaaaaccct cagtcgtttg accgagggtt cctttttatt attcaagaac
     1261 ttgaggctct ataatatttg tagtgggtaa atcccctatg gatattatgg agcctatttt
     1321 gttgtagaaa aaaagtccca tatgacctat aatgaaaagc gacaaaacaa ctcattagaa
     1381 agaatcatat ggaacaatta cattttatca caaaactgct cgatattaaa gacccaaaca
     1441 tcaagattct agatatcatc aatatggata cccacaaaga aattatcgct aagctggatt
     1501 atgaggc
//
LOCUS       DAVMEF010000245         1505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVMEF010000245 DAVMEF010000000
VERSION     DAVMEF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.246"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            41..1444
                     /locus_tag="VYQ30_001984"
     CDS             41..1444
                     /locus_tag="VYQ30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV1967027.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQKLESALKQYNGP
                     YILSDKRWDEVPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYLYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT          549 a          191 c          307 g          458 t
ORIGIN      
        1 aaagtactaa gtaagtctgc cttttaattg gcacgaagta atgaataatg atgagtgggt
       61 ttatcaatac cctattggaa aatttgttga aagacaaggg tggaaaatac acattagttc
      121 agagtataat agttcacacg aattgttaca agatgtagca aaaatttgcc atgaaatgcg
      181 aattccattt aaacatctta gtacagaaga caaatttatc atgcgaaacg ggaaattggt
      241 tagtcgagga ttttctggta aatttattac atgttatcca aatcaaaatg aattagagtc
      301 ggttttgcaa aagttagaaa gtgccttaaa gcaatataat gggccatata ttttaagcga
      361 taaacgttgg gatgaagtac caatatatct gaggtatggt gtgtttaggc cgtcaaggga
      421 tgatgaaaag aaggttgcta ttgatgaatt aattgttggc gatgaagttg ttaaagatga
      481 acgtttacca gtatttaaaa ttccaaaagg tatagtgccc cctgactttt tgaataaatg
      541 gcttgacaag aaagataaaa aacagggaga ctttcccttt attatagata atgcaattag
      601 attttcaaac agtggtggga tatataatgc tagactaaaa gaagatggga aaaaaataat
      661 tttaaaagag gctagaccgt atacaggtct aggatttgat ggtacttatt cttcagagag
      721 attagcttcg gagtgcaaag cactaaaaat tctaaatgaa tggagtgaga tgcctaaaat
      781 atacttgtac ggtaagatct gggagcacac cttcttagga attgaacata tgaagggcgt
      841 tccactaaat agatgggtta ccaataattt tcctttatat gaagttgttg ataagaccaa
      901 agattatcta ttgagagttt caaagatagt agaaaaactc atcgatttaa cgaataagtt
      961 tcattctgaa aatgtatatc atcaagattt acatttagga aatatcttgg ttaaggatga
     1021 agatgagatt agtataatag actgggaaca ggctgtcttt tcgaatgatg aaaaagttgt
     1081 acataaagtt gcagcccctg gttttagagc atggcgagaa acacttccca gtgagataga
     1141 ttggtatggt attagacaaa tagctcatta tttatacatg ccactagtaa ctacatcgga
     1201 tttgacatac aactatgtaa gtcaaaccag gatagaagga aaaaaactat ttgagtcttt
     1261 gggttataca agagaacata tagattatgt agaaagttta ttgtcatatc tagattctaa
     1321 atgtccacag attgagaata tttccaggaa aaaagtttta aaacccatgc atgagattag
     1381 aactatcgag agtgagcaag atatacaaga ttttattata aaagcttttc ttttagtgaa
     1441 ctgataggta ttttcttagc aaacgataaa gttttagggc cgatacaatc aattgcctat
     1501 tcatt
//
LOCUS       DAVMEF010000246         1474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVMEF010000246 DAVMEF010000000
VERSION     DAVMEF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.247"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..463)
                     /locus_tag="VYQ30_001985"
     CDS             complement(<1..463)
                     /locus_tag="VYQ30_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967028.1"
                     /translation="MSANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VYQ30_001986"
     CDS             complement(453..650)
                     /locus_tag="VYQ30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967029.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDVS"
     gene            complement(654..1040)
                     /locus_tag="VYQ30_001987"
                     /pseudo
     CDS             complement(654..1040)
                     /locus_tag="VYQ30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1062..>1474)
                     /locus_tag="VYQ30_001988"
     CDS             complement(1062..>1474)
                     /locus_tag="VYQ30_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1967030.1"
                     /translation="RFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNK
                     EPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLHYSNAKLEATNNLIKLIK
                     RNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT          475 a          254 c          242 g          503 t
ORIGIN      
        1 aaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatgtaagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcaggatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctga catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctaatatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaatccgt
      841 tttttgatgg gattaaataa acatgacacg gattttaaga ataattcttt ctttgacacc
      901 gtatatgtgt aaagcttatt ttgcttgttt aaccaatata aagctttaaa agtcctcaaa
      961 cacctataaa ttgttgagtt tggaagtcca gtgacttgtg aaagcaaatc aacatctact
     1021 actttttggc tctttgtcaa ctgtagtggg ttgaaaaaaa gctaagcttg agaaaggaca
     1081 aattttgtcc tttctttttt gatattcagg gcgataaaaa tccgtttttt gaagttttca
     1141 aagttccgaa aaccaaaggc attgcgcttg ataagtttga tgagattatt ggtcgcttcc
     1201 agtttggcat tagaatagtg tagttgaagg gcgttgacga ttttctcttt gttctttaga
     1261 aaggttttaa agacagtctg aaaaatagga tgaacctgct tcagattgtc ctcaatgagt
     1321 ccgaaaaatt tctccggttc cttattctga aagtgaaaaa gtaagagttg atagatctga
     1381 tagtggtgtt tcaagtcttc tgaatagctt aaaatcttgt caagaatttc tttatttgtt
     1441 aagtgcatgc gaaaagtagg gcgataaaaa cgtt
//
LOCUS       DAVMEF010000247         1468 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVMEF010000247 DAVMEF010000000
VERSION     DAVMEF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.248"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..78
                     /locus_tag="VYQ30_001989"
     CDS             <1..78
                     /locus_tag="VYQ30_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator (MGA)"
                     /protein_id="HEV1967031.1"
                     /translation="IYSNNINTVSLIYLLNAMMFIRLDE"
     gene            440..1441
                     /locus_tag="VYQ30_001990"
     CDS             440..1441
                     /locus_tag="VYQ30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1967032.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
BASE COUNT          549 a          200 c          273 g          446 t
ORIGIN      
        1 atctattcaa ataatataaa cacggtctca ctcatatact tgttaaatgc catgatgttt
       61 attcgattag atgagtaacc aaaaataaca aaaaaacgct atttcgcgtt tgaactgtac
      121 ctcaaaagtt agacagaaaa agtctaattt ttagggtgtt tttgttaaga aaagaccttc
      181 aatcatttaa gaaaaatgta catctagatt tatagccctc taaaactctt gctttttttg
      241 tagaagatac gaaaaaagcc atgaaaccaa ggcttttcct gttttatgta gatgtttaca
      301 catgaaaatt gaagtatttt caaaagctaa ttggagtgat tttattcatg atgggataaa
      361 tgatctggcc aaaaatgatt gagaaatagt tgcttaaaaa gcatttgcat gatactatat
      421 attgaaagga ggtgataata tggtagaaca aagaaaatca attaccatga aagatgttgc
      481 tttagaagca ggagttagtg ttggaactgt ttcacgtgta attaataaag aaaaaggcat
      541 taaagaagta actttgaaaa aagtggaaca agcgattaaa actttgaatt acattccaga
      601 ttactacgct agaggaatga aaaaaaatcg aacagaaacg attgcaatca ttgtaccaag
      661 tatctggcat cccttctttt cagaatttgc tatgcatgtg gaaaatgaag tctataagag
      721 aaataataaa ttactcttat gttctatcaa tggtacaaat agagagcaag actatctgga
      781 gatgttgcgt cataataaag ttgatggagt ggttgccatt acctatagtc caattgaaca
      841 ttacttgacg tcaggaattc cctttgttag tattgaccgc acatactcag atattgccat
      901 tccttgtgtt tcatcagata atgatgcagg tggaagagaa gcagctaaac aattaattag
      961 taaaggatgt cagcatttag cctttgtggg cggacataat acaaccatta atgaaaccaa
     1021 acgtcgtaga atttcatttg aaaagtatgt ccaagagcat aaaatacctc ttagtatttt
     1081 tgatttggat gagacagtta ctgactatta tggaaagcta gagagtttct tggaagaaaa
     1141 ttcttctata gatggaattt ttacaatcaa cgattttaca gctttggatg tgattgaaat
     1201 actaaaaaaa aatggcaaac gtattcctca tgatgtccaa attattggat acgatggaat
     1261 taaaatggct ggagatagag attatctact ttcaactatt gaacaaccat tggaagaaat
     1321 ggcaaaagaa gcggttcgta ttttgtttga tataattgat ggaaagactg ttaatttgca
     1381 gacaatccta ccagtaaaat ttgttgaagg aaaaacaaca aaaaatgaaa ataagtcttg
     1441 acagaaagcg ctatcaatga tagaatga
//
LOCUS       DAVMEF010000248         1467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVMEF010000248 DAVMEF010000000
VERSION     DAVMEF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.249"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1452)
                     /gene="pfbA"
                     /locus_tag="VYQ30_001991"
     CDS             complement(<1..1452)
                     /gene="pfbA"
                     /locus_tag="VYQ30_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1967033.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLG"
BASE COUNT          446 a          290 c          201 g          530 t
ORIGIN      
        1 tcctaaatat tctgcactat cttttgcaac tcgtatcgct tttgttttag gatcggcaat
       61 attaaatata ttttcggcga taaccacctg tttatttaaa tctaataggt ctttagtgtt
      121 tttatagcta taagcattta ctaaagctgc tccgctttct cgataatgta cactctctcc
      181 tttaactttc ttatcaaagc gatttccttt gattaataca tcagtgaatc ctgtaaaacg
      241 tacacctgca tacatcatgt tatcaaaatg attatttaaa attttaatat tagaggggtt
      301 ctgtgtcgac aatgtttgat agtgtgtgcc aattgctgtt actaattccc cagatttatc
      361 acttttgcca aaataggaat tttgaatagt cacattttca gattttttcc catcatcatt
      421 caaggcataa ggaaaacctt ttctagttaa tggttcaatc tgaatgctct ccttacttat
      481 gatttgccca tccttcatcg ttttgggtaa ggcttgccca agaaaacgag aattatcaac
      541 taatacattt ttcgaacctg caatttgaat agcatgccct tgataactat ccttgaatgt
      601 tacatttttt atagttacgt tatttgaatt cccaatagca aatgcacctg aagaatttat
      661 aagtggtaga ttttttgctt tagttccttc ttcattcaaa gcaccgttca tatcaatcgt
      721 accaccagaa taactaatat cttctgttgg gccccattct acttgcgcac catcatccgt
      781 aaataaacct gtcataaaaa caatggaagg gtgattctta atatttatac catttagaat
      841 tgtagctttc tcattcaatt ctaagtgtgt atgacttttt aaaaaaacaa tttcttttac
      901 taaataagtt ccttcaggaa aatatacatt tcctccacct agcccttgag ctgcagcatc
      961 tattgcatct tgaattgctt gtcgatcatc atgaatccca tcacctaccg caccataatc
     1021 ttttacactt aaagcagtat cttcaaattc ttttttgata attctgtcac ttataacatc
     1081 ttccttataa ctcttatttt cattagtaga tgttactgta gattcaactg ttctgttgtc
     1141 taattgtgaa ttggaacgaa tagtaccatt agagctgata ctattaacac ttaacgaatt
     1201 atctaaacca ttagtggttg aattagaggc aatcatttca gcagaacgat tctgattaac
     1261 agttggtgaa ttatctaaat cattagtaat tgcattagaa gactcttttg tagccatcgg
     1321 tgaagaacta gtaacaactt catccgataa aataccttgg cttcccaaaa ttgaaattgc
     1381 caatagtacc gagcaagtcc ccacctttaa tttacgaata ctgaatacct cattaggaac
     1441 aaaatatttc atcagaacct ccaattt
//
LOCUS       DAVMEF010000249         1453 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVMEF010000249 DAVMEF010000000
VERSION     DAVMEF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.250"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            15..1439
                     /locus_tag="VYQ30_001992"
     CDS             15..1439
                     /locus_tag="VYQ30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1967034.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          441 a          269 c          339 g          404 t
ORIGIN      
        1 aggaggacaa gaatttgaca agatatcaga atttagtaaa tggaaaatgg aaatcatctg
       61 aacaagaaat tacgatttat tcaccaatca atcaagaaga attgggtaca gttccagcca
      121 tgactcagac tgaagctgat gaggctatgc aagctgcgcg tgcagccctg ccagcatggc
      181 gagctttatc agcagttgaa cgtgcggctt atttacataa aacagcagct attttagaac
      241 gcgataagga agaaattggt actatccttg ccaaagaagt agcaaaaggg attaaagcag
      301 caattggaga agtagtgcgt acagcagact tgattcgtta tgctgctgag gaaggtctcc
      361 gtatcactgg acaagcaatg gaaggtggtg gttttgaggc agcaagtaaa aacaaactgg
      421 ctgttgtccg tcgtgaacca gttggtatcg tgctagcgat tgctcccttt aattatccag
      481 ttaatttatc tgcttctaaa attgcacctg ccttgattgc agggaatgtg gtcatgttta
      541 agccaccaac acaaggttcc atttctggac tcttgttggc taaagcattt gaagaagcag
      601 ggattccggc aggtgttttc aacaccatta caggtcgtgg ttcagaaatt ggggattata
      661 tcattgagca caaagaagtc aacttcatca actttacagg ttcaactcct attggagaac
      721 gtattggtcg tttagctggt atgcgtccta tcatgttgga acttggtggg aaagatgcag
      781 ctcttgtact agaagatgca gatttggaac atgctgccaa gcaaattgtt gcgggagcct
      841 ttagctactc aggacaacgt tgcacggcca ttaaacgtgt cattgttctc gaaagtgtag
      901 cagataaatt agctactttg cttcaggaag aagtttctaa attaacagtt ggtgatccat
      961 ttgacaatgc tgatattaca cctgttattg acaatgcttc agccgacttc atttggggct
     1021 tgattgagga tgcacaagaa aaagaagctc aggctcttac accaatcaaa cgtgagggca
     1081 atcttctctg gccagtgctt tttgaccaag ttacaaaaga tatgaaagtg gcatgggaag
     1141 agccatttgg tcctgtttta ccaatcattc gtgtggctag tgtagaggaa gctattgcct
     1201 ttgccaacga atctgaattc ggccttcaat catcagtctt tacaaatgat ttcaaaaaag
     1261 cctttgaaat tgctgaaaaa cttgaagtag gtacagtcca cattaataat aaaacccagc
     1321 gtggtccaga taatttccca ttccttggtg tcaaaggttc tggagctgga gtgcaaggaa
     1381 ttaaatatag cattgaagcg atgacaaatg tcaaatccat tgtttttgat gtgaaataac
     1441 gtgtaaaacc agg
//
LOCUS       DAVMEF010000250         1438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVMEF010000250 DAVMEF010000000
VERSION     DAVMEF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.251"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            23..754
                     /locus_tag="VYQ30_001993"
     CDS             23..754
                     /locus_tag="VYQ30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967035.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDVSLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLIP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            772..>1438
                     /locus_tag="VYQ30_001994"
     CDS             772..>1438
                     /locus_tag="VYQ30_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HEV1967036.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWAILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRDELPDLVITANIQSILLSVFIVPTI
                     VGIGAYFYSSRILKISSILKPKKKKRKVTVTGFVNISVRLFLWLLCIGSIVSAGFIRN
                     KEII"
BASE COUNT          494 a          213 c          245 g          486 t
ORIGIN      
        1 actggaatca ggaggattta ttatggttaa taatgtagct gttaaagtct caaacctttc
       61 aaaagaattt ttgttagggc aagacaaaac tgtctctatt ttgaaagatg tttctttatc
      121 tgtcaattat ggggaatttg tatcaatact tggtgtcagt ggttctggaa agtctacttt
      181 attaagttgc ttatcaagtt tatctgaacc aacaagtggc gaagttgtta tcaatggtgt
      241 aaatccatat accttaaaag aagggaagct tgctaaattt agaagacaag atattgcaat
      301 aatttttcaa aactataatc tgatacccgc tctacctgta ctagaaaatg ttacacttcc
      361 tctgagactc tcaggaaaga gtgttgatag caataaagta aaaaaaatgt tggatagttt
      421 gaattttaaa gcagaactat catcattagt tgctacctta tcaggtggag aacagcaaaa
      481 agtggctatt actcgtgcaa taatagctga tagtaaaatt atttttgctg atgagccaac
      541 aggagctctg gacagcgtct caagaaaact tatatttgaa acacttcgta atttagcaag
      601 tcaaggaaaa tgtgttctta tggtcactca tgatattgag ttggcttcaa aaactgacag
      661 agcactcatt ttaaaagacg gaaaaatatc tcgacaaatt attaaacctt cagctgatga
      721 gctctaccaa gcacttgaaa gtagtaaaga ttaatttgag gaggatttat tatgctaaaa
      781 ttaatcattt accaatttca gtactcaaaa agacaatggt taggaacaat accattacta
      841 tttgtatcta gcctaattgt tggaacatct ttatttggta ttgctagttc aatcaagact
      901 gctaacatta acgcttcaca actttttcaa atgttaataa tttttggcgg aactactcta
      961 tttttcctta tttcaaataa tataagactg ctaatagata tctttaaaaa ggattaccaa
     1021 ttatgggcta tcttaggcgc aagcagaact cagctatctt tattagtatc tggacagttt
     1081 tatttaatgg cagtgattgt tagtagtatt ggtacaatac tttcatttat catggcagat
     1141 agttactaca aatttttaca aaatttatta ggaagagatg agttacctga tttagttatt
     1201 acagccaaca ttcaatcaat tttattgagt gttttcatag tcccaacaat tgtcgggata
     1261 ggggcttact tttattcaag tcgtatactt aaaatatcat caatattaaa accaaaaaag
     1321 aaaaaaagaa aagtcacagt aactggattt gttaacatct ctgttcgttt attcctatgg
     1381 ttactatgta taggttctat tgtttcagca ggttttatca gaaacaaaga aataattg
//
LOCUS       DAVMEF010000251         1425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVMEF010000251 DAVMEF010000000
VERSION     DAVMEF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.252"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            180..941
                     /locus_tag="VYQ30_001995"
     CDS             180..941
                     /locus_tag="VYQ30_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1967037.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(1220..1408)
                     /locus_tag="VYQ30_001996"
     CDS             complement(1220..1408)
                     /locus_tag="VYQ30_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1967038.1"
                     /translation="MPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINP
                     EEWDTISFDSDFMPFQQS"
BASE COUNT          498 a          239 c          236 g          452 t
ORIGIN      
        1 ctccaccact cttaaccatc aaaaagtaaa ctagaaacaa caaaataata acttaataaa
       61 aatatcgaaa gttttgaaaa taaacctcga tatttttgtt tttgtttcat tttgttgttt
      121 ttgtatttga atgatgagtt tgtttatggt aagatagtag taggaaacga ggtgataata
      181 tgctcaaaca agaaaaatta accaaaattt tagagatagt aaatagcaaa ggaaccataa
      241 cagttaaaca gattatggac gaaatagccg tttcagatat gactgcaagg cgctatttac
      301 aggaattagc tgataaagat ttgctgattc gtgtgcatgg tggagctgaa aaacttcgaa
      361 ccaactccct tttgactaat gagcgatcaa atattgaaaa acaagccctc caaacggcag
      421 aaaaacaaga aatagcccat tttgcaggca gtctagtaga agaaagagaa actattttca
      481 ttggaccagg aacaacatta gagttttttg cgcgtgagtt gcctattgac aatatccgcg
      541 tcgtaaccaa cagtctacct gtttttctga ttttaagcga acgaaaatta acagatttga
      601 ttttgatagg tggaaattat cgcgatatta caggtgcttt tgttggcaca ttgaccctac
      661 aaaatctctc taatctccaa ttttctaaag ctttcgttag ctgtaatggt attcaaaacg
      721 gagctctagc tacttttagc gaggaagagg gagaggctca acgcatcgct ttaaataatt
      781 ctaataaaaa atatttactc gcagatcata gcaagttcaa taagtttgat ttttatactt
      841 tttataatgt atcaaatctt gatactattg tttcagattc taaactaagt gattcaatcc
      901 tttttaagct atctaaacac attaaagtca tcaagcctta ataaactgaa agagcctggg
      961 taacaactca atttcagaag aaaaaaagta aatcttccca caataaaacg catagtatca
     1021 ggtgttgaaa cacctgatac tatgcgtttt tctatatcaa agactttttg cccaattcct
     1081 caaagctgtg cattgagaaa tctgcgactc gcttcctagt ttaaagttgc tctttgattt
     1141 tcattgagta taagcaacgt gattagataa gagttttaca gagtactaaa tataagattt
     1201 aacttttttg agaaattggt tacgattgtt gaaacggcat aaaatctgaa tcaaaggaga
     1261 tagtatccca ttcttcagga ttgataaaat ttaaaaatag atttttaaat tcttccagct
     1321 catcatctga atgacaaatc cattctttac cagtcgagac ataccatttt ccaagacttt
     1381 tcaattcttc actcgtcaaa cacggtattt gagagcattt ttcga
//
LOCUS       DAVMEF010000252         1421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVMEF010000252 DAVMEF010000000
VERSION     DAVMEF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.253"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(237..1346)
                     /locus_tag="VYQ30_001997"
     CDS             complement(237..1346)
                     /locus_tag="VYQ30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1967039.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          532 a          258 c          208 g          423 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatctac tataaatagg tttcattcga actttaatag
       61 actatgaaaa ccagtggaac ttaccctgac acagaattcc actggttttt tcgattttta
      121 tcagattaac ttctactatt cttagctgtg aaaattagtt taagacttta acttattttc
      181 agtcttctaa tctaaaggcc acttccagta aagaaaaatc agggttctcc tgacttttac
      241 ttagctaatt ctctttctcg ttctttcatt attttatgat tttcatacaa acgggataag
      301 aacttagaaa tttctttcaa gatagaatag gttggcactg cgacaatcat tccaactaca
      361 ccatagatat tgcttgacaa caaaagtaaa actaaaatcg tgattggatg aaccttcata
      421 acacttccta cgattcgagg atataagata ttgccatcta cctgctgaac aacaagcata
      481 taaatcactg caatcagcag tctatgggga tcagtgaata tatttgcgat gatcatagga
      541 atcaaaccaa tacttggccc cacataagga attaaattgg ctacaccaga aaaaatggca
      601 aaaactaaag catattttaa accaataata ctatagccaa tataagccaa acaacctata
      661 atgattgcgt caatcgaaac tccactaata tagcgagcaa tcgtcgcatt taaattcttt
      721 aataagcctg caatatgcaa gcgatccctc tttagaatcg ttctttcaag catgggcaag
      781 aatttatgtc catctaataa gaaataaacc aaaaaaactg gagtcataat caaaatcaaa
      841 acagtactga taagagctga caagacgctc cccacactat ttgatacgct atttaggata
      901 ttttgaagaa tatcaacata ggataagttt aactgctgaa ttgtagcttc tacatccaaa
      961 ttctggagcg caggataatt agataagtct atgattaagt cttgtactcg actataaata
     1021 gtttgactag atataatcaa actagataac tgattaatca aaataggtaa gagatagaca
     1081 acacctatga ccattcccca gaccaaagta cacaaggtaa ttaaaatacc aagcaaacga
     1141 ttgagtttac agactttatt taagaaagta acaatagggt ttgtcaaata ataaagaaag
     1201 ccccctaata aaaatggaat cataattgta ttaagcacgc taacaaaagg gttaatcaaa
     1261 gaccccatct gtctccatag gtaaaagatg atggttaaga gtaaaatttc tgtggtccaa
     1321 aaaaataatt tatttctacg aaacatgagt tacctccctc actttatttt accatatttt
     1381 caaaaagctt aacagatgaa ttattaaagc aagaatattt t
//
LOCUS       DAVMEF010000253         1413 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVMEF010000253 DAVMEF010000000
VERSION     DAVMEF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.254"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..473
                     /locus_tag="VYQ30_001998"
     CDS             <1..473
                     /locus_tag="VYQ30_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967040.1"
                     /translation="GKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELI
                     DIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGISI
                     DDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            473..1234
                     /locus_tag="VYQ30_001999"
     CDS             473..1234
                     /locus_tag="VYQ30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1967041.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKETARLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          515 a          199 c          282 g          417 t
ORIGIN      
        1 ttggaaagaa cataaaatcc ttacgtaaaa cacatgactt aacacaacct gaatttgcac
       61 gaattatagg aatttctcga aatagcttga gtcgttatga aaatggaact agttcagtct
      121 ctacggaatt aattgacatc atttgtcaga agtttaatgt atcttatgtc gatattgtag
      181 gagaagataa aatgctcaat cctgttgaag attatgaatt gactttgaaa attgaaattg
      241 tgaaagaaag aggtgctaat ctattatctc gactctatcg ttatcaagat agtcagggaa
      301 ttagcattga tgatgaatct aatccttgga ttttaatgag tgatgatcta tctgacttga
      361 ttcagacaaa tatctattta gtagaaaatt ttgatgaaat agagagatat agcggctatt
      421 tggatggaat tgaacgtatg ttagagatat ctgaaaagcg gatggtagct taatggaaat
      481 ccaagattat actgatagtg aattcaaaca tgctttagca aggaatcttc gttcactgac
      541 aagaggaaaa aagtccagta aacaacctat agcgattttg cttggaggtc aaagtggtgc
      601 cggtaagact acaattcatc gtattaaaca gaaagaattt caaggaaata ttgttatcat
      661 agatggtgat agttttcgtt ctcagcatcc acactattta gaactgcagc aagaatatgg
      721 caaagacagt gtagaataca ccaaagattt tgcaggtaaa atggtagagt ctttagtaac
      781 agaattgagt catttgggat acaatctttt gatagaggga actttacgaa cgattgacgt
      841 tccaaaggaa acagcacgac tcttgaaaaa taagggatat gaagtacaat tggccttaat
      901 tgcgacaaag cctgaattgt cgtatctaag tactcttatc cgttatgaag aactgtacat
      961 tatcaatcca aatcaagcac gcgcaactcc aaaagaacat catgatttca ttgtaaatca
     1021 tctagttgat aatacacgac aattggaaga actggctatc tttgaaagaa ttcaaattta
     1081 ccaacgagat agaagttgtg tatatgattc aaaagaaaat acaacttcag cagcagatgt
     1141 tcttcaagag ttactctttg gggagtggag tcaggtagag aaggagatgt tgcaggtggg
     1201 ggaaaagaga cttaatgaat tacttgaaaa ataaacaatt gatattttta ggagaataga
     1261 aatgagaggg tttaataaca agataaagtc tgtttatcaa gaactaacaa attccaaaga
     1321 gaaattcggt agctttcaca agactttaat tcatttgcat acacctgttt cttatgatta
     1381 caagctattt tctaattgga ctgcaacgaa ata
//
LOCUS       DAVMEF010000254         1407 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVMEF010000254 DAVMEF010000000
VERSION     DAVMEF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.255"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            18..>1407
                     /locus_tag="VYQ30_002000"
     CDS             18..>1407
                     /locus_tag="VYQ30_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1967042.1"
                     /translation="MVKLNKVLKGSVALLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDQTLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSKHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEEL"
BASE COUNT          506 a          220 c          263 g          418 t
ORIGIN      
        1 aaaatggagg tcttattatg gtaaagctta ataaggtatt aaaagggagt gtagcactcc
       61 tgagtgcaag tttattgcta gcagcttgtg gcaataacgc taattcaaag cctgctgaaa
      121 aaacagctga tggtaaaacg aaaatttcgt ttctatctac atggcaaatt aatgatgttc
      181 gtccacctaa agatcaaaca cttgttggtc agtggttaga agaacagcta ggtattgata
      241 ttgatcttgt aactattcct ggcgagggag taagttctaa agtaaattct cttattacta
      301 gtaaccagtt accgactgtt ttgttgacaa caggagataa gagtgatatc gctggtatca
      361 acaaacttgg taaacagggt gcattcttag atttgtcaaa acacatggac aaactaccaa
      421 actataagaa gtaccttgaa aaaaataatg cattagctca aatagaagat gatgaaaaac
      481 atatttatgc tttcacaaaa ttctttactg atgagaatat catgtatact acaccaattc
      541 tgagaaaaga cttacttgtt ggttcagaat ttgaagatct ttcaaaaatt aaaactgttg
      601 atgactatac aaaagttttg aaatatttga ctgaaaaaca aggttctcca gcatttattc
      661 aacgtaatgg ctatgaaggc ttcatgaagc gagttacacc attgtggaat ttaagccatc
      721 gtacatacta tgactatgaa tcagattcat ataaacatcc agttgaacaa cctcaattga
      781 aacaattcgt tgaatggttg aaagaattac gtaaagataa cgtacttcac cctgactggg
      841 cagttatgaa agatgaaact tgggaaggtc tattggcttc taacaagggt tcattcactg
      901 ttgaccgtat gaatatcatt ggagataata actttgataa atcatttgat tggcagccac
      961 ttgtttatcc agaaattaat ggaaaacgtt atttgcaacc gaaaacaccg tacatttctg
     1021 attcaggttg ggtaatcaat gccaatgcac caaaagaaga aattgataaa gcgttagaat
     1081 tctttaattt cttatacgct gatgaaaatc aagaaaaact tgctattgga tttgaaggta
     1141 aaacatttac acgtgaaaac cctaatactc aagcaggtat taaatggttg attcaaattt
     1201 acggtgaaaa tgctggaaat gataaagcag aactgctgtt taaacatggt aaccaagcat
     1261 ttactcgcgt tcaaactaaa ttagatattg aggcacagcc aggaacattc cctaaagtta
     1321 tgtatgatca agtagataaa attaagaaag atttaggtgg ttttagacca gatactccgg
     1381 ttctttcctt tacaactgaa gaattag
//
LOCUS       DAVMEF010000255         1393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVMEF010000255 DAVMEF010000000
VERSION     DAVMEF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.256"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            326..>1393
                     /locus_tag="VYQ30_002001"
     CDS             326..>1393
                     /locus_tag="VYQ30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1967043.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSILWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKEKYDFKKLTSAYSYLRKNNI"
BASE COUNT          646 a          188 c          234 g          325 t
ORIGIN      
        1 agatgaaata acataattgt cccttggtta caagggcgca attatacacc ctaaagggtg
       61 cttgcgtcct gcggagtcaa acccttgcag ggagcaacaa ccattcgctt tttaaaagtc
      121 aagggtaaat aaactcgcta aggctcgccc ttgactttta aaatttgaaa tgaaagtaac
      181 aattaagccg acatactgaa acaacaaaaa ttaattcagt agtcggcttt ttttattcta
      241 tcatttcaaa acgctcattc acaaagagga tataaaaaaa ctattaagcc tccacctaaa
      301 acaacaaaga aaggaagtga taaaaatggc agacagtttt catttttcag taaacataat
      361 atcaagagga aaaggcaaaa gtgcagtagc aagtgcagca tatataagtg gagaaaaaat
      421 aaaaaatgaa tgggacggag taacccatga ctacacaaga aaagaaaaaa tattagtaaa
      481 aaatataata ttgcctgatc atataccaaa agaatttaat gataggtcta tcttatggaa
      541 taaagtagaa atggcagaaa aaaattctaa tgcacaacta gcaagacaat tcataatagg
      601 actaccaaaa gagttatctt taagtgaaaa taaaaacctg gttgaaagat atataaaaga
      661 aaatttaaca tcacaaggaa tgatagtaga ttatgcaatt catgatgaga gtcaagataa
      721 aaatggaaat atccattgtc atataatgac cataatgcga ccaataaacg aaaaaggaga
      781 gttcttagcc aagtcaaaaa aagaatatat cctagatgaa aaaggagaaa aagtattaaa
      841 caaaaatgga aaaccaaaga caagaaaagt agaactaaca acctggaacg atacaggcaa
      901 tgtagaacaa tggagagaaa atttttcaga tctttgtaac aaatatttag aaagagcagg
      961 agcagaaaaa agagtagacc ataggagttt taaaagacaa aattcagatt atctaccgac
     1021 aatccattta ggatcagcag caagtgcaat ggaaagaaag ggaatagaaa ctgacaaggg
     1081 aaactataat agagaaataa gaaaatataa tcaacttgta aaaacaataa aagaagagat
     1141 aaaaacatta aagggttgga taggaaattt attagataac ttatctactg cttatgaaaa
     1201 atttaaggat atcgaaagag ataaggtaat agacaaccct aaacttttta atctaacaaa
     1261 ttatctatta acttattcag aaattcaaaa agaaaaaagt aaatatctaa aaggatatgc
     1321 aaaaactaat aaagaaaaat atgacttcaa aaagctaaca agtgcatata gttatttaag
     1381 aaaaaacaac ata
//
LOCUS       DAVMEF010000256         1386 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVMEF010000256 DAVMEF010000000
VERSION     DAVMEF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.257"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            119..1297
                     /locus_tag="VYQ30_002002"
     CDS             119..1297
                     /locus_tag="VYQ30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967044.1"
                     /translation="MTLPVRKSLHNAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT          469 a          276 c          292 g          349 t
ORIGIN      
        1 gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacagtgtt ttgagctgac
       61 tgcggctagc tttctagttt gctctttgat ttttattgag tattagaaag gagacctcat
      121 gacacttcca gttagaaaat ccctacacaa tgcggtttta caggcttcag aagcggatac
      181 ttgggaacaa gctaccaagg aatggaatga agtttccttg atttttaacg gtatcggccg
      241 tagcaattgt gtctgtggaa atgccatcaa atacgcttat gaactcttta acggtgtcac
      301 aggccaacgc ctctttccaa ttggtagcga ctgtgttcgt cattttcatc gattgtccct
      361 tgaccagcaa ttggaagagg aagaaaaact gctcagaaag gttgaaaatc taaccagaaa
      421 agctcagaaa aaggaaaaaa tcaaggtcaa taaaagtgac tttgacgagc gacttctaaa
      481 atggctctgg gaaaaaggtg ttttcaaacc caatcgtggc aatcaatttg cgcctgagag
      541 agactaccag cttttcctag aagtctttca gggaggaagt tggaccaagg cggaaccaaa
      601 gaaaaaggct cggatggaag aagtccttga aaagtgtatc aaaccctttt tacttgggaa
      661 atcggatgac caactctacc ttgtcaagct aggcaaggag aaaattgact acgaacaaga
      721 actccgtatc caggcagaga aagaacgcaa gaagagggat aaaatcgcca agcaatatgc
      781 agacaatctg gttctagcca tgggacccgc agagcgtgct tatcaagatt actttggctt
      841 tacagagacc ctgactcaag aagaacgcaa gtgggaaaaa attctttttg gtaaaaatag
      901 agcagaacga gctatcaagg ccaaacaata ccaaaaagag ctggaaaaag accaacgaat
      961 tgccagtcag gatccaattg aaagaaaaca gaagcagact tggatcctca attcctattt
     1021 tcgtgagctt cctgaagaaa aagctcgatt ttctagactc ttattagaat atcgaaaaag
     1081 tggtgaagta cccttttcaa ctgaatacct gtcagatcat ctcatcgact ttttctataa
     1141 aatgaaagcc tttgaatttg aaatcgcacc agaacaagtc cgagattttc taaaaaaaag
     1201 cctgcaggaa gaccatcgct catcagcaca aggaagctgg atagaaggaa ttctcctcaa
     1261 ctgcctcaaa ccatttttag aacgattagt gatatgagta acacctaccg tgtaaatttc
     1321 atggtaggtg ttattgtacc cccaaataat ataaaacaaa gtatgaaagc gcatctcaat
     1381 tttaag
//
LOCUS       DAVMEF010000257         1385 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVMEF010000257 DAVMEF010000000
VERSION     DAVMEF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.258"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..1364
                     /locus_tag="VYQ30_002003"
     CDS             30..1364
                     /locus_tag="VYQ30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1967045.1"
                     /translation="MKLWDLILLGIGAMVGTGVFTITGTAAATLAGPALVISIVISAL
                     CVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAGWLTMMEFMTAISGVASGWA
                     AYFKGLLSQYRIAFPQALNGTFNPQAGTFVDLLPILVLVLVTSLVLLNAKAALRFNSI
                     LVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGASTGIMAGASLMFFGFLGFE
                     SISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLTGVVHYSHLNVDDAVAFALR
                     SVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLARDGLVPAAFKELTKTSKIPK
                     NATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAYGLIRLRKEKGMPKAGEFKT
                     PLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSIIYFTYGYKHSTIEE"
BASE COUNT          326 a          267 c          306 g          486 t
ORIGIN      
        1 gtttagataa aacagagatg cataggcatt tgaagttatg ggatttgatt ttgctgggta
       61 tcggagccat ggtagggaca ggcgtcttta caatcacagg tactgcagct gcaacacttg
      121 ctggcccagc cctagtgatt tcaatcgtta tttctgcctt gtgtgtggga ttatcagccc
      181 tcttttttgc agaatttgcc tcgcgagtac ccgctacagg aggtgcctat agttacctct
      241 atgctatctt aggagaattc cctgcctggt tggctggttg gttaaccatg atggagttca
      301 tgacagccat atcaggcgta gcttcgggtt gggcagctta ttttaaaggc ttgctctctc
      361 aatacaggat agcctttcct caggctttaa atggtacctt taatcctcaa gcagggacat
      421 ttgttgattt attgcctatt cttgtcttgg tcttggtgac ttcgcttgtt ttactaaatg
      481 ccaaggcagc cttacgcttt aattcgattc tagttatttt gaaattttcc gctttagctc
      541 tctttgtttt agtaggaatt tggaatatta agtttgataa ttggagcaat tttgctcctt
      601 acggttttgg acaaatctat ggagctagca ctggtattat ggctggtgcg tccttgatgt
      661 tcttcggttt tttgggattt gaatccatct ctatggctgt agatgaagtc aagactcctc
      721 aaaaaaatat tcctagaggg attgtcttat cgctttctat cgtaaccatt ctctatgcct
      781 tggtgaccct tgttttgact ggtgtggttc actatagtca tttaaatgtc gatgatgccg
      841 ttgcctttgc ccttcgtagt gttgggatta gttgggcagc caactatgtg tcattagtgg
      901 ctatcttgac cttgattaca gtttgtattt cgatgaccta tgccctatcg cgtatgattt
      961 acagtttagc acgtgacggc ttagtgcctg ctgcctttaa ggaactgacg aagactagca
     1021 agataccaaa gaatgctact atcttgacag gtctagcttc agcagtagca gcaggaatgt
     1081 tcccactagc cagtattgca gcctttttaa atatttgtac cttagcctac ttgatcatgt
     1141 tggcttatgg tctgattcgc ttacggaaag aaaaaggaat gcccaaagct ggggaattta
     1201 aaacaccatt ggtacctctg ttaccaattt tatcgatcat catttgttta tccttcatgt
     1261 tacagtataa tatgaatacc tggattgctt tcctagttgc gttgttagta ggaagtatta
     1321 tttacttcac ttatggctat aagcattcta ccatagaaga ataaagtgaa agtctattga
     1381 gttgt
//
LOCUS       DAVMEF010000258         1367 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVMEF010000258 DAVMEF010000000
VERSION     DAVMEF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.259"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..182
                     /locus_tag="VYQ30_002004"
     CDS             <1..182
                     /locus_tag="VYQ30_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967046.1"
                     /translation="REDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSFLKQLAKLY
                     QVTTDYILEMDKFSC"
     gene            313..465
                     /locus_tag="VYQ30_002005"
     CDS             313..465
                     /locus_tag="VYQ30_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967047.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            632..>1367
                     /locus_tag="VYQ30_002006"
     CDS             632..>1367
                     /locus_tag="VYQ30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1967048.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTV"
BASE COUNT          508 a          185 c          286 g          388 t
ORIGIN      
        1 taagagaaga caaagatttg tcccagtgtc aaattgcaca gtatcttcat tgttcacagt
       61 ctgcttattc tagaatagaa agtggcaagc aagatattcc tacatcattt ttgaaacaat
      121 tagctaaatt atatcaggta actacagatt atattttaga gatggataag tttagttgtt
      181 aataaatcct aagccctttg aaaactgaat aaaccgaggt gggactttct tatttgtcga
      241 gcactttagc ttgatacgcc gtgataggag cgataattat tagctataaa atagagtggt
      301 tctctatctg ccatgactac aaataaggat aatgatactt ctgaacgttc aggctgccag
      361 cgaaaaagga cagcgggtga aatgcccatg aatgatttaa ccagtcatac ccacggagga
      421 aattatacat gttacaagga ggaaaaagtt tggaacaatc agtaataaag tatactatgc
      481 aactatcaat gcttaacttt ttatactcaa gaaatatgtt atctaaaaat gagtatgaca
      541 agattagaaa caaactgcgt attaagtaca caaaatagct gactttttga tttgaatgcg
      601 gtaatataga atcaaaagga ggaatttcag tatgaaaaaa aataatgttg aaataataaa
      661 agctgattct ttagttcgtc gaagaggcga caatgtagaa agacacttaa agagggttgc
      721 tgcttattgc agagttagtt ccgatagtga ggatcagaaa aacagttatg attctcaagt
      781 aagacactac aaagaatata tttctcagcg ttcagattgg gagctggcag atatttatgc
      841 agatgaaggt atttcaggaa cacaagtagg aaagagacaa gattttcaac gattaatcaa
      901 tgattgtgca aatggagaga tagattatat tgtcacaaaa gcaattgcta gatttgcaag
      961 gaatacccta gatactttga agtatgtcag aatgttaaag gatatgcaaa ttggagtata
     1021 ttttgaggaa gaaaacatag ataccctaac aatggatgga gaattgcttc taactattct
     1081 aagctccgtt gcacagcaag aagtagagaa tacttctgcc catgtgaaaa aaggattaaa
     1141 aatgaagatg caacgtggtg aattggttgg ttttcaaggg tgcttagggt atgattatga
     1201 tgtagaaacc aagcagattt caattaataa aaaagaagcc aaaattgttc gttatatttt
     1261 tgaaaggtat ctagaaggta ttggaggaaa agtaattgca agggaacttg atgaacttgg
     1321 ttacaaatcg ccaagaggtt tagaacactg gaatgataca acagttc
//
LOCUS       DAVMEF010000259         1354 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVMEF010000259 DAVMEF010000000
VERSION     DAVMEF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.260"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1282
                     /gene="phtE"
                     /locus_tag="VYQ30_002007"
     CDS             <1..1282
                     /gene="phtE"
                     /locus_tag="VYQ30_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1967049.1"
                     /translation="FTLANGQKRVSFSFPPELEKKLGINMLVKLITPDGKVLEKVSGK
                     VFGEGVGNIANFELDQPYLPGQTFKYTIASKDYPEVSYDGTFTVPTSLAYKMASQTIF
                     YPFHAGDTYLRVNPQFAVPKGTDALVRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGT
                     TRTAGNKIPVTFMANAYLDNQSTYIVEVPILEKENQTDKPSILPQFKRNKAQENSKLD
                     EKVEEPKTSEKVEKEKLSETGNSTSNSTLEEVPTVDPVQEKVAKFAESYGMKLENVLF
                     NMDGTIELYLPSGEVIKKNMADFTGEAPQGNGENKPSENGKVSTGTVENQPTENKPAD
                     SLPEAPNEKPVKPENSTDNGMLNPEGNVGSDPMLDPALEEAPAVDPVQEKLEKFTASY
                     GLGLDSVIFNMDGTIELRLPSGEVIKKNLSDLIA"
BASE COUNT          534 a          202 c          267 g          351 t
ORIGIN      
        1 ctttactcta gccaatggtc aaaaacgcgt ttcttttagt tttccgcctg aattggagaa
       61 aaaattaggt atcaatatgc tagtaaaatt aataacacca gatggaaaag tattggagaa
      121 agtatctggt aaagtatttg gagaaggagt agggaatatt gcaaactttg aattagatca
      181 accttattta ccaggacaaa catttaagta tactatcgct tcaaaagatt atccagaagt
      241 aagttatgat ggtacattta cagttccaac ctctttagct tacaaaatgg ccagtcaaac
      301 gattttctat cctttccatg caggggatac ttatttaaga gtgaaccctc aatttgcagt
      361 gcctaaagga actgatgctt tagtcagagt gtttgatgaa tttcatggaa atgcttattt
      421 agaaaataac tataaagttg gtgaaatcaa attaccgatt ccgaaattaa accaaggaac
      481 aaccagaacg gccggaaata aaattcctgt aaccttcatg gcaaatgctt atttggacaa
      541 tcaatcgact tatattgtgg aagtacctat cttggaaaaa gaaaatcaaa ctgataaacc
      601 aagtattcta ccacaattta aaaggaataa agcacaagaa aactcaaaac ttgatgaaaa
      661 ggtagaagaa ccaaagacta gtgagaaggt agaaaaagaa aaactttctg aaactgggaa
      721 tagtactagt aattcaacgt tagaagaagt tcctacagtg gatcctgtac aagaaaaagt
      781 agcaaaattt gctgaaagtt atgggatgaa gctagaaaat gtcttgttta atatggacgg
      841 aacaattgaa ttatatttac catcgggaga agtcattaaa aagaatatgg cagattttac
      901 aggagaagca cctcaaggaa atggtgaaaa taaaccatct gaaaatggaa aagtatctac
      961 tggaacagtt gagaaccaac caacagaaaa taaaccagca gattctttac cagaggcacc
     1021 aaacgaaaaa cctgtaaaac cagaaaactc aacggataat ggaatgttga atccagaagg
     1081 gaatgtgggg agtgacccta tgttagatcc agcattagag gaagctccag cagtagatcc
     1141 tgtacaagaa aaattagaaa aatttacagc tagttacgga ttaggcttag atagtgttat
     1201 attcaatatg gatggaacga ttgaattaag attgccaagt ggagaagtga taaaaaagaa
     1261 tttgtctgat ctcatagcgt aaggaatagc agtagaaaaa gtctgaatca aaaatgaagt
     1321 tctctcaaaa gttagaaata aaactctgac tttg
//
LOCUS       DAVMEF010000260         1317 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVMEF010000260 DAVMEF010000000
VERSION     DAVMEF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.261"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(51..374)
                     /locus_tag="VYQ30_002008"
     CDS             complement(51..374)
                     /locus_tag="VYQ30_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1967050.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            complement(364..1019)
                     /locus_tag="VYQ30_002009"
                     /pseudo
     CDS             complement(364..1019)
                     /locus_tag="VYQ30_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            1090..1167
                     /locus_tag="VYQ30_002010"
                     /pseudo
     CDS             1090..1167
                     /locus_tag="VYQ30_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT          460 a          306 c          271 g          280 t
ORIGIN      
        1 acaccattat atgatatata tatctgtgat aggttttctt tatggctgat ttaaaagacc
       61 aaacgcaaga cggcaatcaa gactactcca aagagaactg ttccgactag attgcggtag
      121 cgaaaggcta cccaagctgt tggaaagacg gctaagaagt ctagccattt aatttgagga
      181 aggctcccaa ccttacctgt cactacgctt gaaagaatca aggcaaagat aatggaaacg
      241 ggcaagaact tcaaaaaacg ctcaacgatt gcaggcaagc ccttatactt gactaagatg
      301 aagggaatca tacggagaat ccaagtcacc aagccagaga aaataactgc taataaaaga
      361 tacttactga ccatctaaaa ccacccccat gctacaacca agtagcgtcg caaacagaac
      421 agctagtgac tgagacacca ctgtcaagag caaaaaaagg acaccgcaac aactgctagg
      481 ataatgagca gattgcggac aggaatccgt ctttgcataa tctgaaattg cgaagcaaaa
      541 ataccaataa acatcccaac cagggcaaaa tccaagccaa agatttctgg atttggtagc
      601 aggccaccca gagccgttcc gactactgtc cccacaaacc aagccacata gctgttgaga
      661 ttgtttccgt gcatccacat aggatttacc ttgtctgtat gggccaattc acccatcaaa
      721 acgccatagg tctcatctgt caagatacta gacataccga tattgtacca aagactggta
      781 tgacggaaat aagtcgatgc gtgtaaactc aacaaaaaga gacgcaagtt gattagaaaa
      841 accgtcatag caatagctgc cacaggagct tgaaccacaa tcagtgccaa catggcaaac
      901 tgggcactcc cagcataaac aaagagactc atcaagccca tctcaacagg tgtcacatag
      961 ggcgcaccga tagtcccaca ggccaggccg atactgacat aaccaagagc cgttggcatg
     1021 gctgcctgcg ccccctccta aaatcctttt tctttcatct ttctcctcat attgtcttaa
     1081 taatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaaacat
     1141 tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacctacgg caaggtgaag
     1201 ctgacgtggt ttgaagagag tttcgaagag tacaagtagg ctgaaaagaa tccagccaca
     1261 gcatggacta ttatatagca gattgaaata agatgagaac aaatcgattg ggaaagt
//
LOCUS       DAVMEF010000261         1313 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVMEF010000261 DAVMEF010000000
VERSION     DAVMEF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.262"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYQ30_002011"
     CDS             complement(87..1283)
                     /gene="tuf"
                     /locus_tag="VYQ30_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1967051.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          348 a          273 c          290 g          402 t
ORIGIN      
        1 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
       61 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
      121 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
      181 atcaactcaa catcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
      241 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
      301 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
      361 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
      421 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
      481 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaacg
      541 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
      601 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
      661 cgttctggtt ctgggatata ctcatcaact gtgttcatca attccataac gatgtcttcg
      721 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
      781 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
      841 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
      901 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
      961 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
     1021 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
     1081 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
     1141 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
     1201 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
     1261 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttt
//
LOCUS       DAVMEF010000262         1309 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVMEF010000262 DAVMEF010000000
VERSION     DAVMEF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.263"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..441)
                     /locus_tag="VYQ30_002012"
     CDS             complement(<1..441)
                     /locus_tag="VYQ30_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967052.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFNPIISNTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSI"
     gene            complement(438..791)
                     /locus_tag="VYQ30_002013"
     CDS             complement(438..791)
                     /locus_tag="VYQ30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1967053.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(847..>1309)
                     /locus_tag="VYQ30_002014"
     CDS             complement(847..>1309)
                     /locus_tag="VYQ30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967054.1"
                     /translation="LVFNKTEFLFNQNKWQKEDSFESDLTKTLKDKIQKSELSYSDLM
                     SLLLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCTGLVELLLYEISISKNHNWSEK
                     IDKIRIQLIRINQLDFFYYTSWLRQNFDFCMNREYHKMKSRKLLLSNKKIV"
BASE COUNT          455 a          232 c          176 g          446 t
ORIGIN      
        1 aatggatgga aatatagtta atataaaact agaaatctct aaatatttta tccaaggaga
       61 ttgagacacc ttgcttctga actgactaat tttcttatat tcgttagtca atttagtagc
      121 aaaatcttcc ccaattaaat gaaagattgc taactctagg ataaaaacac ttattaatac
      181 ggtataagtc aaaaaacttt ctgggaaaaa cttattgatt aaaatgggga aactgattaa
      241 cagaatcgaa tttatggcta gacagataga tactatttta tttttcacag aatatagagg
      301 tagtgatact attgagtaga taaaagctaa aaaaataagc aaaaaatact ccgtgttact
      361 gattatcgga ttaaatgata aaaatatata catccaacat acgataatgt gtatttttcg
      421 aatcttatct acattactca tcttactgtc gcagatatga atccatccca aggataaggg
      481 ttcccgttaa tatcacaacc tctattaaga acaccatatc caatacagta agctattttc
      541 cctgctgctg tagctaatac acctgctgct gcaccaataa gccagcctcc aagacctcca
      601 actttaatat agtttattag ttttttggtt aaaactgcag ctccagctaa tgctataaaa
      661 gttcctgaac tcatagctaa agctgcgcaa attccttgac aagcacgctt gcttaaataa
      721 gctcccccat caagatacat catttcttct tcatcaatca caacataatt atttggtaat
      781 acaagttcca tttgttttct cctccttagt tgaaattgtt tcatttgttt tacaaatagg
      841 acgaacttaa actatctttt tattactcag taataatttc cgagatttca ttttatgata
      901 ctctctattc atacagaaat caaaattttg ccttaaccaa cttgtataat agaaaaaatc
      961 taattgattt attctgatga gctgtattct aattttatca atcttttctg accaattatg
     1021 attcttacta atagaaattt catacagcag taactcgaca agacctgtac acagaagggt
     1081 gttatgagaa tcttcgattt ttttcaaaat atcctcaaaa agttcactat ccgttaaaaa
     1141 ggtggattgg ttcatcgcat catagagaag aagggacatt aagtcagaat agcttaattc
     1201 tgatttttgt atcttatcct ttaacgtttt cgtcaaatca ctttcaaacg aatcttcttt
     1261 ttgccatttg ttttgattaa aaagaaattc cgtcttatta aaaacgaga
//
LOCUS       DAVMEF010000263         1305 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVMEF010000263 DAVMEF010000000
VERSION     DAVMEF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.264"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..1221
                     /locus_tag="VYQ30_002015"
     CDS             43..1221
                     /locus_tag="VYQ30_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1967055.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          484 a          238 c          195 g          388 t
ORIGIN      
        1 tcataggatt ttaagaatct gacaactagg aagaaaacta atatgacctt atctaagaaa
       61 caattacaac taagagctaa aatactggaa acagtttaca cattaggtcc catttcacga
      121 attgaaatcg ctactaaaac aggaataaca cctgcaacaa ctagcagtat cacaaatgat
      181 ctgattaaag aaaatattct cttagaactt ggtgaagacg aacacgatac aagcgtaggg
      241 agaaaaaaaa tcctgctaga cattcaagct aaacgatttt actacattgg ttgtgaacta
      301 tctgaaaaac atttcacttt tgctttagga gataatctag gaaatatctt aaaggaagag
      361 aaagaaattg ttactaaaca gttaatccaa gaaaagggaa atcaactcat taaccagaca
      421 ctaaagcaat tcttaaataa ctgctctgat tatgaaatag aagcaattgg aatcgcgcta
      481 cctggtcgct atttagatga ttataaaata acaacaaaca atcctttatg gcaacatatc
      541 gacttagaaa tgattcaatc ccactttgat aagcctttgt ttttctctaa caatgttaac
      601 tgtatggcta taggtaaacg cctatttagt cgccaacaga atgaccctaa ttttgcttat
      661 tttcattttg ctagaggtat gcactgttct tatatctacg acggaaacat ttatgggaaa
      721 ggaaatctaa tgattggaga aatagggcat acagttgttt catctgaggg agaagaatgt
      781 agctgcgggc gcaagggatg cttacaaact tttgctggag aatcttggtt aatcaaaaaa
      841 tcaaaaattc tatatcatca atctccctat tctctacttc ctagtttagt caaaaatgcc
      901 gatgatattg atatccaagt tattttaacc gcttatcaat taggtgacac tggaataatc
      961 acacttatcc atcaagcctt actctatctt agtcaaacca ttttaaatat cagtatgatg
     1021 attgactctc aaaaaatcta cctacatagt cctttactaa caaatcaaca catcatccaa
     1081 aaactatata gtgaaatgaa ctacaaaccc aaactgctct ataatcgcct acctgaagtc
     1141 attattgaac cttacaatga ttttacagcc gcacactcag ctattgcact ttgtttgtat
     1201 catacaattc tacattctta attttctatt acatttaata aataggctaa aaagagggag
     1261 tatatcataa aaagcgaata aacctgtttt ctgaaaatca gaatt
//
LOCUS       DAVMEF010000264         1290 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVMEF010000264 DAVMEF010000000
VERSION     DAVMEF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.265"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..320
                     /locus_tag="VYQ30_002016"
     CDS             <1..320
                     /locus_tag="VYQ30_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967056.1"
                     /translation="IDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMND
                     VERRQLISALISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLE
                     KRS"
     gene            588..1154
                     /locus_tag="VYQ30_002017"
     CDS             588..1154
                     /locus_tag="VYQ30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967057.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT          464 a          177 c          283 g          366 t
ORIGIN      
        1 aaatagatga attagaatca tcactaattg atgcgaaagc aaagaaacag actattgaag
       61 ctgaaaaact tacaggagat aacatatata aggttctgat ctattttgat aagctctata
      121 aagtcatgaa tgatgtagag cgtaggcagt taatttcagc tttgatttct gaaattcaag
      181 tttatgaaga aaaacaatct aacggacaat ggctaaaatc cattactttt aagctaccaa
      241 ttatagaaga aaatctcaac atagatttgg acaatgatga acaagttgaa tgtgtaagtt
      301 tattagagaa acgtagttaa tcctcaaaag gttccccaaa ctttttgagt cccactgacg
      361 catcacgctg aggcggtatc actgcttgta cgagttgaag catcagcgaa gtaaaagtag
      421 atgttcagcc aatgcgacga ggtgagaccg aaggtcgata aggtgtgttc tgggtatagg
      481 actcgtaaag taagtaggaa atatgatgag atgatacaga gagcctgaat tggtcaggtg
      541 acgtgcttat ttacattagc ctgataatga aaatggagag attatcgatg gttatattgg
      601 aattgtacca aaatgattat tctaaagatt tagtagcatt tgattccata gaagatggga
      661 aagcctttgt ggctcaaatc ccgggataca ctctggaaac agaagatggt tttgaagtgg
      721 agtattttaa tcctaaaaac ataccggatt atatggaaat tatttttaac ggaaatattg
      781 ttcctttatc taaatttatg ttcgatcctg aggaaaatgt aaacattatt tggaaagaga
      841 tttcaaattt atccctcaaa aatgatagag tgatcgaagg atattcaaaa attgatgcat
      901 atgtcgttaa caaccatgaa gttaaagcct atgtcgaaac aagagaaacg aactatcgta
      961 aagcaaaaga cttcttagaa agccgcggat atgaaatcga cagaagcttt ttcggaagtg
     1021 aagacggtga agcgattctt tacagaaaaa aagggatcga agtttggcat ttcttgtgtc
     1081 acttagaccc aatgtttgta gagattgaag atgtagaagg atatgttaaa gaagaagttg
     1141 gagagattca atagtcaacc aaagttcctt gtgagtatat acctttgcta gtaaaagagt
     1201 caaaacgtta taacgaaggg ggggaagaaa acccttgagt gtaagtcttt ttcttgttta
     1261 gagggaaagt attagagtag ccgctcataa
//
LOCUS       DAVMEF010000265         1280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVMEF010000265 DAVMEF010000000
VERSION     DAVMEF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.266"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..722)
                     /locus_tag="VYQ30_002018"
     CDS             complement(<1..722)
                     /locus_tag="VYQ30_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967058.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKI"
     gene            complement(1037..>1280)
                     /locus_tag="VYQ30_002019"
     CDS             complement(1037..>1280)
                     /locus_tag="VYQ30_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967059.1"
                     /translation="IIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVI
                     NEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          463 a          218 c          176 g          423 t
ORIGIN      
        1 tttatttttt taaatcgctt aacttctaat aaatcaattt gctcacgcac gctctctgaa
       61 agatatggct tgatcaaact gatataagat aaagccacgc atggcaactc agattttgat
      121 tgcaatttcc aaatttcatg ataatcctga agattaaact cacaatccct cttagattcc
      181 agtattgtta gtacctttga ttttaacaga aataattgtt gattctcaat tctataaagt
      241 ttttgtacta atgctatgat aatagaaaat ggaataagag gcaaaagcaa cggtttgtca
      301 tacacaaaag taaagaaaaa atagtatgaa attagtgttg taaagtacaa gaagtagtaa
      361 aatttaaaca gattcttagt tgtttgactc tccttataaa acactgctat tcctggacta
      421 aatcccatta gttggtgaaa gagaattcga tagtatttcc tagctcctat aatgatcaca
      481 aatataaaat aaaaataaat tggtcttaat ttacaaaaga ttgaggaaac tagtaatagc
      541 aaagtatgta agcattgaga caaaatatag aataacggta tcgccaatac aaaggttaag
      601 atggcatgag agggagttcc taagatgtcc aattttataa atactgggct gacacaaaat
      661 atcgtgctta taagcagtaa aattattaaa attgtgtcaa aaaaataacg cttatttttc
      721 atttagtcta ctttccattg ttaaataata ctactgctaa caccaagcca acttagcaca
      781 ttgcatcacc taatcctacg ctacttgtcc ataatccttt aatgtttcta ctaaaattat
      841 tccaatctcc cccatcaaga tagcttattt atcctagaca acgcctactg caacagtata
      901 aataagattc atcagccatc ttttctggca atcatatggg tagactaatg atatttctag
      961 ttactcaaaa atatctagct atactagagt tatttctaat ccctaggaca cttgttaatc
     1021 caaatgtaac aaattttcac cccttaatag gactctttct ggaaaaaata taaggtctat
     1081 ctttataaaa aaatctatca tattgggatg gcacgcatct ggaatttaaa caagctataa
     1141 attcatttat aactactatt tgtttagagg caacagcatc ttgtataccg ccctcgaaag
     1201 aaccatcttg taattccgaa atatttttgt ttaaatattg ttttgacaaa tctcttaatg
     1261 cttcattatc gtaaatgatt
//
LOCUS       DAVMEF010000266         1257 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVMEF010000266 DAVMEF010000000
VERSION     DAVMEF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.267"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1257
                     /locus_tag="VYQ30_002020"
     CDS             <1..>1257
                     /locus_tag="VYQ30_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1967060.1"
                     /translation="SELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGIRIINTDD
                     SDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQINKVIKR
                     QFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQLLELVYKET
                     SFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEGVAQSFE
                     SEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQISVK
                     YQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDIKKEL
                     SHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVA
                     ETLSYYCSNNFELEVWTELELSKESLE"
BASE COUNT          415 a          180 c          207 g          455 t
ORIGIN      
        1 tctgaactag cagagttatt aaattgtaca gaacgtgcag tcaaagatga tctatcccat
       61 gttaaatctg cttttcctga cttgattttt cattcttcta ctaatggtat acgcattatt
      121 aataccgatg atagtgatat tgaaatggtt taccatcatt tctttaaaca ttcaactcat
      181 ttttcgattt tagaattcat cttctttaat gaaggttgtc aagctgagag tatttgtaaa
      241 gaattttata tcagttcatc ttcgctctat cgtattatta gccaaatcaa taaagtgatt
      301 aaaaggcaat ttcaatttga agtcagtctg acccctgttc aaatcattgg aaatgagaga
      361 gacattcgtt acttttttgc acaatatttt tcagaaaaat attatttcct agaatggcca
      421 tttgaaaatt tttcatcaga gccactatct caattgttag aattggttta taaggaaaca
      481 agctttccaa tgaatttgtc aacgcataga atgctaaagt tgctcctagt tacgaaccta
      541 tatagaataa agtttggtca ttttatggaa gtagataaag attcttttaa cgaccaaagt
      601 ttggattttt taatgcaggc agaaggaata gaaggtgttg ctcagagttt tgaatcagaa
      661 tacaatatct ctttagatga agaagttgtt tgccaattat ttgtgtctta ctttcaaaaa
      721 atgtttttca tagatgaaag tctctttatg aaatgcgtaa aaaaggatag ttatgttgaa
      781 aaatcttacc atctattgag tgattttatt gatcagattt cagtcaagta tcagattgaa
      841 atggagaata aggataatct gatttggcat ctgcataata ccgcacatct gtatcgtcaa
      901 gagttgttta ctgagtttat tttatttgat caaaaaggga atacaatcag gaactttcaa
      961 aatatttttc caaaatttgt ttcagatata aaaaaagagc tttctcacta tttagagact
     1021 cttgaggttt gttctagttc aatgatggta aatcatctat cttatacctt catcactcat
     1081 accaaacatt tggtgattaa tttgctacaa aatcagccta aactaaaggt tttggttatg
     1141 agtaattttg atcagtatca tgcaaaattc gttgcagaga cactttctta ttactgtagc
     1201 aataattttg aactggaggt ttggaccgaa ttagaattat caaaggaatc tttagaa
//
LOCUS       DAVMEF010000267         1247 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVMEF010000267 DAVMEF010000000
VERSION     DAVMEF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.268"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1247
                     /gene="pcpA"
                     /locus_tag="VYQ30_002021"
     CDS             <1..>1247
                     /gene="pcpA"
                     /locus_tag="VYQ30_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1967061.1"
                     /translation="AVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTV
                     EGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETG
                     RIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELP
                     ASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNL
                     FRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELASY
                     SFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELI
                     LPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVK
                     KDTFAIPETVKFYVTSEHIKDVL"
BASE COUNT          477 a          179 c          204 g          387 t
ORIGIN      
        1 gcggttattt tagcagcata tgtccctaat gaaccaatcc tagcagatac tcctagttcg
       61 gaagtaatca aagagactaa agttggaagt attattcaac aaaataatat caaatataag
      121 gttctaactg tagaaggtaa cataggaact gttcaagtgg gtaatggagt tactcctgta
      181 gagtttgaag ctggtcaaga tggaaaacca ttcacgattc ctacaaaaat cacagtaggt
      241 gataaagtat ttaccgttac tgaagtagct agtcaagctt ttagttatta tccagatgaa
      301 acaggtagaa ttgtctacta tcctagctct attactatcc catcaagcat aaaaaaaata
      361 caaaaaaaag gcttccatgg aagtaaagct aaaactatta tttttgacaa aggcagtcag
      421 ctggagaaaa ttgaagatag agcttttgat ttttctgaat tagaagagat tgaattgcct
      481 gcatctctag aatatattgg aacaagtgca ttttctttta gtcaaaaatt gaaaaagcta
      541 accttttcct caagttcaaa attagaatta atatcacatg aggcttttgc taatttatca
      601 aatttagaga aactaacatt accaaaatcg gttaaaacat taggaagtaa tctatttaga
      661 ctcactacta gcttaaaaca tgttgatgtt gaagaaggaa atgaatcgtt tgcctcagtt
      721 gatggtgttt tgttttcaaa agataaaacc caattaattt attatccaag tcaaaaaaat
      781 gacgaaagtt ataaaacgcc taaggagaca aaagaacttg catcatattc gtttaataaa
      841 aattcttact tgaaaaaact cgaattgaat gaaggtttag aaaaaatcgg tacttttgca
      901 tttgcagatg cgattaaact tgaagaaatt agcttaccaa atagtttaga aactattgaa
      961 cgtttagcct tttacggtaa tttagaatta aaagaactta tattaccaga taatgttaaa
     1021 aattttggta aacacgttat gaacggttta ccaaaattaa aaagtttaac aattggtaat
     1081 aatatcaact cattgccgtc cttcttccta agtggcgtct tagattcatt aaaggaaatt
     1141 catattaaga ataaaagtac agagttttct gtgaaaaaag atacatttgc aattcctgaa
     1201 actgttaagt tctatgtaac atcagaacat ataaaagatg ttcttaa
//
LOCUS       DAVMEF010000268         1247 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVMEF010000268 DAVMEF010000000
VERSION     DAVMEF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.269"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            302..1165
                     /locus_tag="VYQ30_002022"
     CDS             302..1165
                     /locus_tag="VYQ30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1967062.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQGQMAETYRRLNAIVIKNALHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKSGFLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGNNQKEIENFIQSLQVLENPQLTSLFE
                     LRLQQYKALID"
BASE COUNT          387 a          233 c          253 g          374 t
ORIGIN      
        1 gttctttgta accgctccat tataaaatcc tttcactctt ccggtatact attaggaaaa
       61 acataactgg aggatatttt gagctcacac ctcatcgttc cagtggctgt tccccaagct
      121 cgtgtactgc ccaaaaaagt tagacattct atttagaacg aggattgagt tctgtattgt
      181 acaaggctcg gtccttttag agtcagctta aggctggctt ttttaatcac caaagtgtca
      241 gaatgttttg acaaatgaac acaaataatg atataatatg caaaagctag gaggtggtag
      301 gatgattgaa aaaatggaac tgggggaatt ttacaaggaa ttgcgtttgg cgagaaagct
      361 caagcagtca gatgtggctt gtgctggact aacagcttct cagctatcca agttcgaact
      421 agggcagtct atgctgtctg cggataagct catactagcc atccaaggga tcaatgtgac
      481 ctttgatgag tttgggcaca agctcaacaa ctatcaagaa tctccacata tgcagattgg
      541 tagaaaggtt gtggatcgct ttgctcatca agatatagct ggcttagagc aactgttgga
      601 ggaagtcgag caaggacaga tggcggagac ctatcgtcgt ttgaatgcta ttgtgattaa
      661 aaatgcactc cattccttag ataaaagcta tccgctagca gaggaggata gcgagttttt
      721 gactacctac ctctatgcta ttgagtcttg gacctggttt gaactctatc ttttttgcaa
      781 taccatgccc ttcttgagca atcaagatct gatcttttta tcaacctctt tgcttgaaaa
      841 atccaaggaa tttaaagagt tggtacataa tagactgtat atgaaaagtg gctttctgaa
      901 tattatatca gagctcatgg agcgcaagct tttctcctat atcccaatct ttgaagcaga
      961 gctggagagc atgttgcgtc cgtacgatgt ttttgaaaaa gtattgtggc aatttttaaa
     1021 gaaaatgagt attttccttc aaaccaaagg aaacaatcaa aaagagattg aaaattttat
     1081 ccaatctctg caagtattag aaaatccaca attaacatcc ctttttgaac tacgcttaca
     1141 gcaatacaaa gcacttatcg attagttaga aatgggaaaa agataataat ctcagcaacg
     1201 attgtcaata atgttaaaac ttgtttcttc atatgacttc cactcct
//
LOCUS       DAVMEF010000269         1240 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVMEF010000269 DAVMEF010000000
VERSION     DAVMEF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.270"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            236..676
                     /locus_tag="VYQ30_002023"
     CDS             236..676
                     /locus_tag="VYQ30_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967063.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLIEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            673..1131
                     /locus_tag="VYQ30_002024"
     CDS             673..1131
                     /locus_tag="VYQ30_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1967064.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          396 a          275 c          236 g          333 t
ORIGIN      
        1 ccttgccaga gggagtaaaa tagtggtata ctcaataaaa atcaaagagc aaactagaaa
       61 gctaggcgca gacagctcaa aacactgttt tgaggttgta gataaaactg acaaagtcaa
      121 caactatcct acggcaagac gacgctgacg tggtttgaag agatttttga agagtattaa
      181 taaaggtgta caagaagtga gattaatttt gtatgaaaaa caaggagaga aatttatgaa
      241 agtagaatta cagattagtg agacttacga ggaggaaaag ctgattgtcc aagcacctca
      301 gccgacagat aaagttcaga aagtcatcga gttcgcagaa aatctggacc aaagagaaaa
      361 aatcaaaggg aaaatcgatg atcaggtcta tctagttgag attggcaaga ttcagcgctt
      421 ctatatcgag aatcggaagg ttctagcaga aaccgcgagt cagacctaca gcattgattt
      481 gcgactctat caggttctta aactcttgcc aaccaatttt atccaaattt cccaatcaga
      541 aatcatcaat atcgattcca tctctcacct caagctcacg ccaaacggtc tgatagaaat
      601 tttcttgaaa aacgaaagct tcacctactc ttcacgccgt tatctaaaaa ccatcaagga
      661 gaaattagaa ctatgaaaaa acaagtattt cacgatgcag ctgccggtgt tcttatcggc
      721 ctcatcctct ctatcctctt ttcactcatt tatgcaccaa atacctacgc accactaaat
      781 ccctactctc tcatcggcca agtgatggat cagcatcagg ttcacggtgc cctggtcttg
      841 ctctactgca cacttatctg ggcaaccatc ggtatgctct tcaactttgg caaccgctta
      901 tttagccgtg actggagcat gcttcgtgcc actctgactc atttcttcct tatgctggct
      961 ggctttgtcc cactagcaac tcttgctggt tggttccctt tccactggat tttctacctc
     1021 cagctcatta tcgagtttgc gattgtctat ctcatcatct gggctattct ctataaaaga
     1081 gaggctaaaa aagtagatca catcaatcaa ctcttggagc atagaaaata ggaacctcat
     1141 aataagcaaa tccgagaaag aaacttctcg gtttttcttt atcctgctgc accttactat
     1201 agtgaaatga aataagaaca ggataaatcg atcaggacag
//
LOCUS       DAVMEF010000270         1232 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVMEF010000270 DAVMEF010000000
VERSION     DAVMEF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.271"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            129..1154
                     /gene="trpS"
                     /locus_tag="VYQ30_002025"
     CDS             129..1154
                     /gene="trpS"
                     /locus_tag="VYQ30_002025"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1967065.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
BASE COUNT          402 a          185 c          266 g          379 t
ORIGIN      
        1 gtgtaaatta tatttacagt atcctttaca caaatctgta aaaagcaagg ctgatttatt
       61 ttgataaatt acggttattt cattaaaaaa atgctataat tgaaaggact atatcgaagg
      121 agaacaaaat gactaaaccc attattttaa caggagaccg tccaacagga aaattgcata
      181 ttggacatta tgttggaagt ctcaaaaatc gagtattatt acaggaagag gataagtatg
      241 atatgtttgt gttcttggct gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa
      301 ccattgtaga gtctatcgga aatgtggctt tggattatct tgcagttgga ttggatccaa
      361 ataagtcaac tatttttatt caaagccaga ttccagagtt ggctgagttg tctatgtatt
      421 atatgaatct agtttcgtta gcacgtttgg agcgaaatcc aacagtcaag acagagattt
      481 ctcagaaagg atttggagaa agcattccga caggattctt ggtctatcca atcgctcaag
      541 cagctgatat cacagctttc aaggctaatt atgttcctgt tgggacagat cagaaaccaa
      601 tgattgagca aactcgtgaa attgttcgtt cttttaacaa tgcatataac tgtgatgtct
      661 tggtagagcc ggaaggtatt tatccagaaa atgagagagc agggcgtttg cctggtttag
      721 atggaaatgc taaaatgtct aaatcactaa ataatggtat ttatttagct gatgatgcgg
      781 atactttgcg taaaaaagta atgagtatgt atacagatcc agatcatatc cgcgttgagg
      841 atccaggtaa gattgaggga aatatggttt tccattatct agatgttttt ggtcgtccag
      901 aagatgctca agaaattgct gatatgaaag aacgttatca acgaggtggt cttggtgatg
      961 tgaagaccaa gcgttatcta cttgaaatat tagaacgtga actgggtcct attcgtgagc
     1021 gccgtattga atttgctaag gatatgggag aagtttataa tatgattcaa aaaggtagtg
     1081 aaagagcgcg tgaagttgcg ggtcaaaccc tatctgaggt aaaaggggca atgggacttc
     1141 attactttaa ctaagtttat tttacaagaa actatctttt ctatctcgaa gaaaagatag
     1201 ttttttattt acccctgtaa catttgacaa at
//
LOCUS       DAVMEF010000271         1229 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVMEF010000271 DAVMEF010000000
VERSION     DAVMEF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.272"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..1224
                     /locus_tag="VYQ30_002026"
     CDS             4..1224
                     /locus_tag="VYQ30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1967066.1"
                     /translation="MLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQP
                     VEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVE
                     ELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETL
                     GDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYML
                     EGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATS
                     SLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERVGNQSSQS
                     VQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKIEKAFFLK
                     VWWNQFVRFVNEKL"
BASE COUNT          384 a          250 c          245 g          350 t
ORIGIN      
        1 actttgttaa ctgtctctct tttagggggt gcttctactg ctgttgctca agattttacc
       61 attgccgcta aacatgcgat tgctgttgag gcaaatactg gtaaaattct ctatgagaag
      121 gatgcaacgc aacctgtcga aattgcttcc ataacaaaat tgattactgt ttatctggtc
      181 tatgaagctt tggaaaacgg aagtattacc ctctccactc ctgtagatat ttctgattat
      241 ccttatcaat tgacgacaaa ttctgaagcc agtaatattc ctatggaggc ccgtaattat
      301 actgtcgaag agttgcttga agcaactctg gtatctagtg ccaacagcgc cgctattgcc
      361 ctagctgaga aaattgctgg ctcagaaaaa gatttcgtcg atatgatgcg ggcaaaactc
      421 ttggaatggg gaattcagga tgccactgtt gtcaatacga caggtcttaa caatgaaact
      481 ctaggggata acatttaccc aggttctaaa aaagatgagg aaaataagct tagtgcttat
      541 gatgtcgcta tcgttgctcg caacctcatc aaaaaatacc cacaagtctt agaaatcacc
      601 aaaaaacctt cttctacttt tgctgggatg acaatcactt caaccaacta catgttagaa
      661 ggtatgcctg cttaccgtgg tggttttgat gggctaaaaa caggaacaac agataaggct
      721 ggagagtctt ttgttggtac tactgtcgaa aaaggcatga gagtcatcac agttgtttta
      781 aatgcagatc atcaagacaa taatccttac gctcgattta cagctacatc ttccctaatg
      841 gattatattt cttctacatt tacacttcgc aaaatagttc aacaaggcga tgcctatcaa
      901 gatagcaaaa cccctgtaca agatggaaaa gaagatacag taactgcagt ggctccagag
      961 gatatctatc taatagaacg tgttgggaat caatcttccc aatctgttca attcacacct
     1021 gattccaaag caatcccagc accacttgaa gctggaacag tggttggcca tttgacttat
     1081 gaagacaagg acttgattgg tcaaggttac atcaccacag agcgccctag tttcgaaatg
     1141 gtagcagaca agaaaattga aaaagccttc ttcttaaaag tttggtggaa tcagtttgtc
     1201 cgctttgtta acgagaaatt ataaaaaat
//
LOCUS       DAVMEF010000272         1223 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVMEF010000272 DAVMEF010000000
VERSION     DAVMEF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.273"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(16..>1223)
                     /locus_tag="VYQ30_002027"
     CDS             complement(16..>1223)
                     /locus_tag="VYQ30_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967067.1"
                     /translation="FVSDEASEETPLAERGEKEYIVKSSRMTALCYAESKDGVKWEKP
                     NLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRYKLVSKLEFSPNLHYMVVVFS
                     EDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVITRTWANNVRLSAITFSDDFIN
                     WSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHEGDTLNENYDKVDLELKFSAN
                     LEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPIIEDDKIWFYYMGGNGNHTGF
                     RESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYDDEIYILADIPENGYVKVAVG
                     TKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLGNSPVAFKFEIENAKLYGIEG
                     SIQQHYLKY"
BASE COUNT          383 a          246 c          174 g          420 t
ORIGIN      
        1 aacgaattaa tctggttagt atttgagata gtgttgctga atactacctt caataccata
       61 caattttgca ttctcgattt caaatttgaa cgcaactgga gaattaccta aatctaagat
      121 agatttttta ttaaattcca acttatactt tccatcggaa acttgaacaa gagagatatc
      181 ttcctgacca aaatctgaat aagcattacc gtatttagta cctaccgcaa ctttaacata
      241 accattttca ggaatatcag ctaagatata aatttcatca tcgtaagttt ggaagctaac
      301 tgtagatatt ttagctggaa ttccttctct tttattagta taataagcaa attggtcttt
      361 cttaatatat cctctagcta atgaactttc tcggaagcca gtgtgattac catttccacc
      421 catatagtag aaccaaatct tatcatcttc aattattgga gcagccgtga aaatacaact
      481 agcatcaaaa tcagcattct gcgggtatac tccacctccc ctttcaatga gatagtcgcc
      541 cttacataca ctatcccatt gttccaaatt agcagagaat ttcaattcta agtctacttt
      601 atcatagttt tcatttaaag tgtcgccttc gtggtacatt gaagcaaaac ctaaatagat
      661 atccgcgtat gaaattactg gcatagaata aatttgatct tcaaatccat ctcccctaag
      721 gatttcttta ggttctgacc aattaataaa atcatcactg aaagtaattg cacttagacg
      781 tacgttattt gcccaggttc ttgtaataac agcgtattta ccagtttttg gatctctaaa
      841 tggaaaatta tgactatctg ctgcaggatt ccattttttc catggaatag gttcgtcaaa
      901 atgaataccg tcttctgaaa aaacaacaac catgtaatga agattcggag aaaattctaa
      961 ttttgaaact aatttatatc gtttcttagg atccaactcc tgttcatcta gcatgatacc
     1021 tgcaccgtgt gcatgcatca gaacaatatt attatctttt gaacctttaa attttactag
     1081 attgagattt ggtttttccc attttactcc atctttactt tcagcatagc aaagtgctgt
     1141 cattctagat gacttgacaa tgtattcctt ctctccacgt tctgctaaag gagtctcttc
     1201 gcttgcttca tcactaacaa atg
//
LOCUS       DAVMEF010000273         1210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVMEF010000273 DAVMEF010000000
VERSION     DAVMEF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.274"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(257..>1210)
                     /locus_tag="VYQ30_002028"
     CDS             complement(257..>1210)
                     /locus_tag="VYQ30_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1967068.1"
                     /translation="EEAKESRNEEKVKQAKAKVESKKAEATRLEKIKTDRKKAEEAKR
                     KAAEEDKVKEKPAEQPQPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQ
                     QQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGSMATG
                     WLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGSMA
                     TGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEASGA
                     MKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT          271 a          258 c          238 g          443 t
ORIGIN      
        1 atcgctctga acatcaaaaa agaaaggacg aaatttgtcc tttctcaagc ttagctgact
       61 tcaacccact acagttgaca aagagtcaaa aaaactccgt agtctctagc gctataaatc
      121 cggctagaga ttacagagtt atttttttat ctaaacttcc tacaagggta tattatctac
      181 tgaaaggata tggtatttgt agggaatact ttaaagagtt cttacaggaa gtcagtatta
      241 actagttata ttaggtttag tttacccatt caccattggc attgactcta tagctatcta
      301 cagttgtgtt gactgcaagg gcacctgagc cattgacata gtaccattta tctgatactt
      361 tgaaccattg gcttgctttc atagcacctg atgcttcaag atagtaccag gtatctccat
      421 ctttcaccca atctgtcgcc attgaaccat tagcgtttag gtagtaccat gagccattgt
      481 tttggagcca tcctgtcgcc attgaaccat tagcgtttag gtagtaccat gagccattgt
      541 tttggagcca tcctgtcgcc attgaaccat tagcgtttag gtagtaccat gagccattgt
      601 attggagcca tcctgtcgcc attgaaccat tagcgtttag gtagtaccat gaaccattgt
      661 tttggagcca tcctgtcgcc atatcaccat tagcgtttag gtagtaccat gaaccattgt
      721 attggagcca tcctgttgcc attgaaccat tagcgtttag atagtaccat gagccattgt
      781 tttggagcca tcctgtcgcc attgaaccat cagtattgta gaagtaccac ataccgtttt
      841 cttgtttcca gcctgttttt ggagtagatg gttgtgctgg tttttcagtt tttggcggtt
      901 gctgttgagt caagcgatta tattcttctt ctgatctacg agcatagtct tcttcagctt
      961 gttgatcagc tggtttttct gcttttggtt gttcagctgg attctctggt tttggagctg
     1021 gagctggttg tggttgttca gctggttttt ctttaacttt atcttcttct gctgcttttc
     1081 gtttagcttc ttctgctttt ttacgatctg tcttgatttt ttctaacctt gtagcctcag
     1141 cttttttact ctcaactttc gcttttgctt gcttaacttt ttcctcgttt cgagattcct
     1201 tagcttcctc
//
LOCUS       DAVMEF010000274         1200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVMEF010000274 DAVMEF010000000
VERSION     DAVMEF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.275"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..794
                     /locus_tag="VYQ30_002029"
                     /pseudo
     CDS             1..794
                     /locus_tag="VYQ30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(829..>1200)
                     /locus_tag="VYQ30_002030"
     CDS             complement(829..>1200)
                     /locus_tag="VYQ30_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1967069.1"
                     /translation="KVSGRRYQRISLVAGLTNGELIAPMTYEEMVTSDFFEAWFQKFL
                     LPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNL
                     KKVLPSCNTFYEALFSCSCFN"
BASE COUNT          395 a          250 c          200 g          355 t
ORIGIN      
        1 aaaattcaaa ctagaattct tcttagaaaa ggccgtttta agtgctatca ttgctcaaaa
       61 atggaggttg ctgagacttc tatcgtcaag aagaatcatc aaattccttg tatcatcaac
      121 caaaagattg ctcagaagct aattgaaaag acttctatga ccgacattga tcatcagttg
      181 tctatttcaa cttcaactgt cattcgcaag atcaatgatt ttcactttga gcatgatttt
      241 tcgcgtcttc ctgagattat gtcctaggac gttgaaacag tctggggagt gactgtttca
      301 atcgggagat agagatgagc tttattgctc aggactttga taagctcaat atcataactg
      361 ttcttgagag cagaacacaa gccatcatcc gaaatcccat gaatacaagg ctatcaagcg
      421 atactgaaag ctcattcaac aagatagtca gaaactgagt gataaacgat tttatcgccc
      481 tacttttcgt atgcacttga ctaataaaga aattctagat aaacttttga gctattccga
      541 agacttgaaa caccactata atctctatca gctcttgctt ttccaccctg tttaaaacct
      601 tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc aacgccaaac
      661 tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt tttaggaact
      721 ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg ctttcttcaa
      781 cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc aattgaaaca
      841 agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct ttttgaggtt
      901 ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt agggaggaag
      961 aggtaaaagt ttatgcccaa actcctcgca taaaagttct aacttaccca ttctatggaa
     1021 tcttgcatta tccataataa taaccgatgg tgtggttaat gttggtaaga gaaacttctg
     1081 aaaccaagct tcaaaaaagt cgctcgtcac catctcttcg taagtcattg gagcgattaa
     1141 ctcaccattt gttagacctg caaccaaaga aatcctctga tatcttcttc cagatacttt
//
LOCUS       DAVMEF010000275         1181 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVMEF010000275 DAVMEF010000000
VERSION     DAVMEF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.276"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            108..956
                     /locus_tag="VYQ30_002031"
     CDS             108..956
                     /locus_tag="VYQ30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1967070.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
BASE COUNT          380 a          270 c          185 g          346 t
ORIGIN      
        1 aagcaatttc attctataaa ttggagaatt agttttattt ctactgaatc tcccatctac
       61 ttattcctaa agttgctttc atttgcctct tttgatacac ttaaactatg aatacaaatc
      121 tcaagcccaa acttcagcgt tttgcttctg cgactgcctt tgcctgtcct atctgtcaag
      181 aaaatctgac tctgttagag actaatttca agtgctgcaa ccgtcattct tttgacttgg
      241 cgaaatttgg ctatgtcaat ctagcacctc aaatcaagca atctgctaac tataacaagg
      301 aaaattttca aaaccgtcaa caaatcctag aagccggctt ttaccaagct atcttagatg
      361 ctgtatctga cttgcttgca agctcaaaaa ctaccacaac aattttggat atcggttgtg
      421 gtgaaggatt ctattctcgc aaactacaag aaagtcactc tgaaaaaact ttctatgcct
      481 ttgacatctc caaagattca gtccaaatcg ctgctaaaag tgaacccaac tgggcagtca
      541 attggttcgt tggcgacttg gcacgccttc ctataaaaga cgctaacatg gatattctgc
      601 ttgatatctt ctcacctgcc aactatggag aatttcgtcg cgttttatcc aaagacggta
      661 tcttgataaa ggttatccca actgaaaatc acctcaaaga aatccgtcaa agagtacagg
      721 accagctgac aaacaaggag tattctaacc aagatatcaa ggagcatttc caggaacact
      781 ttaccatcct atctagtcaa actgcctctc tgactaagac tatcacagca gaacaactcc
      841 aagccctact cagtatgact cctctcctct ttcacgttga ccagagcaag attgactgga
      901 gccaactgac agaaattacc atcgaagcag agattttggt tgggaaagca ttctaaacta
      961 gacttcccaa aggtataaaa cttaccgaaa atcttcaata tgacaaaaac gtataagaac
     1021 aggtacacaa accttaatct tatgcgtttt gttatagttt ccatacacat taaggggaaa
     1081 tagttgaaat cagttctttt aataaccatt gaataccata tactacattt accccaaata
     1141 tgatataggc cgtataaatc aagtatttcg atttttcccg c
//
LOCUS       DAVMEF010000276         1170 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVMEF010000276 DAVMEF010000000
VERSION     DAVMEF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.277"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1053
                     /locus_tag="VYQ30_002032"
     CDS             <1..1053
                     /locus_tag="VYQ30_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEV1967071.1"
                     /translation="FFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNKNFRQAINFAFDR
                     TSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYGKEWQGINFADGQ
                     DPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGVSSIKQSIESVLG
                     SDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPSTYLDIFNTNSGG
                     FLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYADIQAWLIDSSLVL
                     PSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDEYAKAREKWLKEK
                     EESNKKAQEELAKHVK"
BASE COUNT          406 a          187 c          250 g          327 t
ORIGIN      
        1 ttctttaatt ttaacctaga taggaagtct tacaattata cttctaaaac aagtgacatt
       61 gaaaagaaat cgactcagga agcagttctc aataaaaact tccgtcaggc tatcaatttt
      121 gcttttgaca gaacatctta tggggctcag tctgaaggga aagaaggtgc aacaaagatt
      181 ttgcgtaacc tagtggttcc tccaaacttt gtcagtatca agggaaaaga ctttggtgaa
      241 gttgtagcct ctaagatggt caactatggt aaggaatggc aaggtatcaa ctttgcggat
      301 ggtcaagacc cttactacaa tcctgagaaa gccaaggcta agtttgcgga agctaagaaa
      361 gaactcgaag caaagggtgt tcaattccca atccacttgg ataagactgt ggaagtaaca
      421 gataaagtag gcatacaagg agttagttct atcaaacaat caattgaatc tgttttaggt
      481 tctgataatg tagtgattga cattcagcaa ttaacatcag atgagtttga cagttcaggc
      541 tactttgctc aaacagctgc tcagaaagat tatgatttat atcatggcgg ttggggacct
      601 gattatcaag acccgtcaac ctatctcgat atttttaata ctaatagtgg aggatttctg
      661 caaaatcttg gactagagcc tggtgaggcc aatgacaagg ctaaggcagt tggactggat
      721 gtctatactc aaatgttgga agaagctaat aaagagcaag atccagccaa acgttatgag
      781 aaatatgctg atattcaagc ttggttgatt gatagttctt tagttcttcc aagtgtttcg
      841 cgtgggggaa caccatcatt gagaagaacc gtaccatttg ctgctgccta tggtttaacc
      901 ggtacaaaag gggttgaatc atataaatac ctcaaagtac aagataagat tgtcacaaca
      961 gacgaatatg caaaagccag agaaaaatgg ttgaaagaaa aagaagaatc caataaaaaa
     1021 gcccaagaag aattggcaaa acatgtcaaa taaaatctaa ttgtagataa gtttgtgtaa
     1081 gaagtctttg tgcttaaaaa cgcataatat caggtatata aaatcctaga tgttatgcgt
     1141 tttgttatag gaagatttat tagattttat
//
LOCUS       DAVMEF010000277         1164 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVMEF010000277 DAVMEF010000000
VERSION     DAVMEF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.278"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..492
                     /locus_tag="VYQ30_002033"
     CDS             <1..492
                     /locus_tag="VYQ30_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1967072.1"
                     /translation="ATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNY
                     FMDLKVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDD
                     ISIIGFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRR
                     QSF"
BASE COUNT          373 a          172 c          241 g          378 t
ORIGIN      
        1 gctacttatg atatgatgaa agctgtaact gaagaaaaat ttaaaaatat gtggttgctt
       61 atgggaggag aatcccatgt taataaggat cgtgaaagag gtgtgcgttc ttttctgaat
      121 gataaaaatt actttatgga tttaaaagtc atttatggct tatctacaat tgattcagtt
      181 tattcttatg caatgcagca cttgactacc caaaattatc cggacattat ttttgtttca
      241 ggagatgaaa aggttcaggg cttgattcgt gcgtgctatg agaaaggaat tcttattcca
      301 gatgatattt ctatcattgg atttgataat attcctattt ctcagtatta tactccagct
      361 ttgtctacaa ttgctcccaa ttatgttaaa ttggctaaag aaatgataga aggtgtttta
      421 gcaattatta aaggggaaag tgtcacatct gttgaagttt ctcctaaatt tgttaggaga
      481 cagagtttct agtattgttt aattcttgaa aatcctagtg gaaaagatgg agtgttctaa
      541 aataagttct gtttagcctc cattcagttt ctattgagtt ggatccaatt tctaacgagt
      601 gggacatgtt catgttcgat ctaaggaaga catggtcaca ttcgaatgat attttcaaaa
      661 aaatcgtaaa aaagtcttga caaagaaaaa acaaagtatt aaaataagtt caacaaatca
      721 gaaaagtaac tatttttgat cttcagggag cctgtggtga ttgtgaatag gtggttggaa
      781 gtagtgaaag tgggctgatt ttgaaaatga atttgaaaca atgaaaattc ggtgcgcaca
      841 ccttacagtg cagcttgttg ttaaacaagg cagagatgta aagggatagt ccctttataa
      901 ttgaggtggc accgcgttac caacgccctc acacggaagt atattctgtg tgtgggcttt
      961 tttctatccg tcgtttggtt tatcttttat tagggcctta agtcgctttg atgaacttga
     1021 gttctatcta cagctccttg cctactacta aaagcaaaca aaaaggctgg actaatacga
     1081 aaaaagctag taatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg
     1141 ctcaaaacac tgttttgagg ttgt
//
LOCUS       DAVMEF010000278         1154 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVMEF010000278 DAVMEF010000000
VERSION     DAVMEF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.279"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            162..>1154
                     /locus_tag="VYQ30_002034"
     CDS             162..>1154
                     /locus_tag="VYQ30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1967073.1"
                     /translation="MYDVVVIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIEKGGTVFSSMEELMNVSDFYQITIPYQPE
                     VYESVLTEMLNESNVDILLNVEINNIVFDNNKIHSVEIISNQEVNYLESKVFIDATGN
                     ATIASASGANVYEKNSSYGCLMRVGGVDITKTLEHIEKTKQWEFEEGYNEWLDNVLAN
                     RKYRATGAVLRDPVSYDHAPMLTKDDHFMNEEKWSYIKERWERFGFIYTLEVSLFRNE
                     LREAVEAGDFEFEIYHDDESGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYME"
BASE COUNT          400 a          140 c          253 g          361 t
ORIGIN      
        1 tgggattcat ttatcaatga agctaaatct aaagacttgg ataaagttac aaaagtatat
       61 aatgatgcct acaaacgtgt caaagataga atgaaataga ttaaatggta gctctctgat
      121 tattcaggga gctactaagc ctagaaagtg aatattcgaa aatgtatgat gttgtagtta
      181 taggtgctgg tagttcaggt gctcttgcag caattgcttc tggtcgccta ggattaaaaa
      241 cctgtttaat cgaaaaaggg agtcgtatag gtggcgtacc gattaatact ctaatgggtt
      301 catttgcaaa tctttacttc gatgattctg ggagagtaaa tgccagtaag attgttggtg
      361 agttaattga acgtattata gaaaaaggtg gaactgtttt ttcttcgatg gaagaattga
      421 tgaatgtaag cgacttttat caaatcacaa tcccgtacca acctgaagtc tatgaatcag
      481 ttctaacaga aatgttaaat gaatctaatg ttgatattct tctgaatgta gaaattaaca
      541 atatagtttt tgacaacaat aagatacata gtgtagaaat catcagtaat caagaagtaa
      601 attatcttga atcaaaagtt tttattgatg ctacaggtaa tgctacgata gcttcagcaa
      661 gtggcgctaa tgtatatgaa aaaaattctt cttatggctg tttaatgaga gtgggtggag
      721 ttgacatcac taagactctt gaacatattg aaaaaacgaa gcagtgggaa ttcgaagaag
      781 gttataatga atggctagat aatgtattgg ctaaccgaaa gtatagagct acaggcgcag
      841 tattgagaga tcccgtttct tatgatcatg ctcctatgtt aactaaagat gaccatttta
      901 tgaatgaaga aaaatggagt tatatcaagg aaaggtggga gagattcggg tttatatata
      961 ctttagaagt atcattgttt agaaatgaat taagagaagc agttgaagct ggtgattttg
     1021 agtttgaaat ttatcatgat gatgaatctg gagtaactat gaacggtgat ggtatcagtt
     1081 atggttcatg gggtcaaaat gtagcattaa ttaatgttgc aaaagcattt ggattcaatc
     1141 ccaaatatat ggaa
//
LOCUS       DAVMEF010000279         1124 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVMEF010000279 DAVMEF010000000
VERSION     DAVMEF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.280"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            71..943
                     /locus_tag="VYQ30_002035"
     CDS             71..943
                     /locus_tag="VYQ30_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1967074.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLPSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
BASE COUNT          352 a          197 c          251 g          324 t
ORIGIN      
        1 tagatataag attacaaaag gaggtctgcg tctccttttt attgtataat aaaagtgaga
       61 ggaaaaaaga atgaaagtga ttaatcaaac cttattagaa aaagttatta ttgaacgttc
      121 tcgttcaagt cataaaggag attatggtcg tctgctgctg cttggtggta cttatcctta
      181 tggaggtgcc atcatcatgg ctgctttagc agctgtaaaa agcggtgcag gattggtgac
      241 cgttggaacg gacagggaaa atatcccggc tctgcacagc catttacctg agcctatggc
      301 cttttctctt caagaccagc aattgttaaa agagcaattg gagaaggcag aagttgtctt
      361 gctggggcct ggtttacgag acgatgcttc tggagaaaat ctagtaaaac aggtctttgt
      421 taatttaagc caaaatcaga ttttgattgt agatggaggt gccttgacca ttcttgctag
      481 gacaagtttg tcatttccct caagccagct tatcctaact cctcaccaaa aagaatggga
      541 aaaattatct ggaattacga ttgaaaagca aaaggaagat gcaacggcta gtgttctgcc
      601 ttctttccct caaggaacaa ttttggtgga gaaaggccca gctactcgta tttgggaagt
      661 tggccaatct gattattacc agttacaggt tggtggtcct tatcaggcga ctgggggaat
      721 gggagatact ctggctggga tgatcgcagg atttgtaggc caattccgac aggccagtct
      781 ctacgagcgt gtggcagtag cgacccatct tcattcagcc atagcccaag aactatctca
      841 agaaaattat gtggtcttac cgacggaaat cagcaggtat cttcccaaaa taatgaaaat
      901 tatttgtcaa caagagaggg taagcaaaga taaacttgtt taaaatagac agataaatct
      961 atttccttct cttaatttat gattcttgaa atgcgcctgc tttaaggcta aactagacgt
     1021 atcaaagtta agattttgtt tttgtaactg gaaagagatg aacaaatcaa atacataaaa
     1081 gataaactca tttttattcg tttggtgaaa agaaaataca tatt
//
LOCUS       DAVMEF010000280         1124 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVMEF010000280 DAVMEF010000000
VERSION     DAVMEF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.281"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            32..694
                     /locus_tag="VYQ30_002036"
     CDS             32..694
                     /locus_tag="VYQ30_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1967075.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          374 a          176 c          191 g          383 t
ORIGIN      
        1 tcgtgaaaag attctttgta ataaatatac catgaacgaa caattgcctg atgaaatgac
       61 attagctaaa cagtttgcct gtagtcgaat gacgatcaaa aaagctttag acttgttagt
      121 ttctgagggc ttaattttta gaaaacgtgg gcagggaacc tttgttctct ctcgtggcag
      181 ctcaaaaaga aaattaatcg ttccagaaag agatatccgg ggactgacaa aaatatctga
      241 agatgctcat tctacaattg actcgaggat tattcacttc aaattagaat ttgcaaatga
      301 atttttagca gaaaaactac aggtcgcttt gcagagtcca gtttataata tttaccgcct
      361 gcgtattatt gacggtaaac cttatgttct ggaacaaact tatatgagta ccgatgttat
      421 tccaggtatt actgaagata ttttacaaaa atcgatttac aattacattg aaggaaagtt
      481 aggattgcat attgccagtg ctacaaaaat cttacgagct tcttctagtt cagaaaatga
      541 gcaacattac ttgcagctcc ttccaacgga accggtattt gaagtagaac aagtggctta
      601 tttggataac ggaactccgt ttgagtactc gattagtcgt catcgctatg atttatttga
      661 atttaattct tttgcattac gacattcctc ctaggagaaa atgtgaaaat gaagccaatc
      721 ttttacagac tctagtttaa gaaaaattta aaacagggca agaaggttcc atctatgctt
      781 aaatggtttc tcttttctaa ataagatggc tttaaaagag tgatcgtttt atccatcatg
      841 ttgaaaaata tcttcgtata gcttatagag taggtactga aattgttcac ttgatctact
      901 tcttatagtt atttagtttt aaatagtgtt tcaaacattc ttacactgac gagaagtttt
      961 tgagtctttt cttgtaacac atatagtata ctgaggttag aatagtagac tgtgacttct
     1021 aaaaaattgc tagaaatgaa tttcaatctc ccaatttatt tgttcatatc ttcttttaat
     1081 atattaaaca aattctgaat cataatcatt taaaaaaatt ttat
//
LOCUS       DAVMEF010000281         1100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVMEF010000281 DAVMEF010000000
VERSION     DAVMEF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.282"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>1100)
                     /locus_tag="VYQ30_002037"
     CDS             complement(<1..>1100)
                     /locus_tag="VYQ30_002037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967076.1"
                     /translation="AEALVDADADALVLAEADALVLAEAEALVLAEAEALVDAEAEAL
                     VLAEADALVDADSEALVDAETEALVDAEADALVEADSDAEVLAEAEALVDAEAEALVL
                     AEADALVDAEADALVLAEADALVDAEAEAEALVLAEADALVDAEAEALVDAEAEALVL
                     AEAEALVLAEAEALVDADSDAEILAEAEALVDAEADALVDAEAEALVLAEAEALVLAE
                     AEALVDADSEAEVLAEAEALVDAEAEALVLAEADALVLAEADALVDAEADALVLAEAD
                     ALVEAEALVLAEADALVLAETDALVDAEADALVDAEADALVDAEAEALVDAEAEALVL
                     AEAEALVLAEAEALVDAEADALVDAEAEALVDA"
BASE COUNT          273 a          365 c          246 g          216 t
ORIGIN      
        1 gcatcaacaa gcgcctcagc ttcagcatca acgagtgcgt cagcctcagc atcaacgagt
       61 gcttcagcct cagcaagtac aagcgcctca gcctcagcaa gtacaagtgc ttcagcctca
      121 gcgtcaacaa gtgcttcagc ttcagcatca acaagtgcgt cagcttcagc atcaacaagt
      181 gcgtcggctt cagcgtcaac cagtgcatca gtctcagcaa gcaccagtgc gtcggcttca
      241 gcaagtacta gcgcctcagc ctcaaccagt gcatcagcct cagcaagtac tagtgcatca
      301 gcttcagcat caacgagtgc atcggcttca gcaagtacta gtgcatcagc ttcagcaagc
      361 accagtgcct cagcttcagc gtcaacgagc gcctcagctt cagcaagcac ctcagcttct
      421 gaatcggcat caacaagcgc ctcagcctca gcaagtacca gtgcttcagc ctcagcaagt
      481 accagtgctt cagcttcagc atcaacgagt gcgtcggctt cagcgtcaac cagtgcttca
      541 gcctcagcaa gtatctcagc gtctgaatcg gcatcaacaa gcgcctcagc ttcagcaagc
      601 accagtgctt cagcctcagc aagtactagc gcctcagcct cagcgtcaac aagtgcttcg
      661 gcttcagcat caacgagtgc gtcggcttca gcaagtacta gcgcctcagc ctcagcttca
      721 gcatcaacga gtgcgtcagc ttcagcaagt accagtgcgt cagcttcagc atcaacaagt
      781 gcgtcagctt cagcaagtac cagtgcttcg gcctcagcat cgacaagcgc ctcggcttca
      841 gcaagtacct cagcgtctga atcggcctca acaagtgcat cagcctccgc gtcaacgagt
      901 gcttcagtct cagcgtcaac cagtgcctct gaatccgcat caacaagtgc atcggcttca
      961 gcaagcacca gtgcttcggc ttcagcatca acaagtgctt cagcctcagc aagtaccagt
     1021 gcctcggctt ccgcaagcac tagcgcgtca gcttcagcaa gtaccagtgc gtcagcatcc
     1081 gcatcaacaa gcgcctcggc
//
LOCUS       DAVMEF010000282         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.283, whole genome shotgun sequence.
ACCESSION   DAVMEF010000282 DAVMEF010000000
VERSION     DAVMEF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.283"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            53..1060
                     /locus_tag="VYQ30_002038"
     CDS             53..1060
                     /locus_tag="VYQ30_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1967077.1"
                     /translation="MLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQSILSH
                     KFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFFNPNY
                     YGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAGAIIY
                     LFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMALFKQN
                     PFWGEGPLTYMNSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRLMMDM
                     SQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLVSDMT
                     D"
BASE COUNT          244 a          188 c          235 g          428 t
ORIGIN      
        1 gatttttaca ggagacatga aaagtattct tcagaaaatg ggggagcatc cgatgctgct
       61 tctttttctt agctatagta ctgttatatc cattcttgca caaaattgga tgggtcttgt
      121 ggcttcagta ggaatgtttc tatttactat tttctttttg cactatcagt cgattttatc
      181 ccataaattc tttcgattga ttttgcagtt tgtcttgttt ggtagtgtct tgtcagctgc
      241 ttttgccagt ttagaacatt tccaaattgt gaagaaattt aactatgctt ttctttcacc
      301 caatatgcag gtgtggcatc agaaccgggc agaagtgacc ttctttaatc ctaattatta
      361 tggaattatt tgttgtttct gtattatgat tgctttctat ctgtttacaa cgaccaagtt
      421 gaattggttg aaagtattct gtgtgattgc aggctttgtt aatctctttg gtttgaactt
      481 tactcaaaat cgaactgcct ttcctgctat tatcgctgga gcaattatct atctctttac
      541 gactattaaa aactggaagg ccttttggct tagtattggg gtcttcgcga ttggtttgag
      601 tttcctcttt tctagtgatt tgggagttcg aatgggtact ttagactctt ctatggaaga
      661 acgcatttct atctgggatg ctgggatggc cttgtttaag caaaatcctt tttggggtga
      721 agggccattg acctatatga actcttatcc tcggatacat gctccttatc atgaacatgc
      781 ccacagtctt tatattgata cgattctgag ttacggaatt gtggggacta ttttattagt
      841 tttgtcttct gttgctcctg ttcgcttgat gatggatatg agtcaggagt cggggaaacg
      901 tccgattatc ggcctttatc tatctttcct tacagtggtt gctgtgcacg gaatttttga
      961 cttggctctc ttctggattc agtcaggctt tattttcttg ctagttatgt gcagtattcc
     1021 attggagcat cgaatgttgg tatcggacat gacggattaa gttttataag tttgaaatct
     1081 tctaccttgg gtagg
//
LOCUS       DAVMEF010000283         1093 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVMEF010000283 DAVMEF010000000
VERSION     DAVMEF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.284"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..342)
                     /locus_tag="VYQ30_002039"
     CDS             complement(<1..342)
                     /locus_tag="VYQ30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967078.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKK"
     gene            complement(388..594)
                     /locus_tag="VYQ30_002040"
     CDS             complement(388..594)
                     /locus_tag="VYQ30_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967079.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(581..742)
                     /locus_tag="VYQ30_002041"
     CDS             complement(581..742)
                     /locus_tag="VYQ30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967080.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT          359 a          216 c          125 g          393 t
ORIGIN      
        1 ttttttactt tctgacaaag gacgatataa acttgtatca tagcctaatc ttgaagctgt
       61 ctctataaca ctaatatcca tatttttaat ttcttctgca tcaaggtatc catcattcct
      121 caatctatat aacatagctt tatgactgat accataaaac tgacccaatt ttataatatc
      181 ttctacttca agatgagttc tattggcatt ttctctgatt tcctcaacca tcctatacag
      241 tgaagatggg aaaattaaaa aataagaagc aaactgatcc gcttttcttt cagtttcatc
      301 tccttcacca atcaagataa gactgactga actcttcttc acctcatcat aataaagatg
      361 atacagtcca tgtgctaaag aaaatcttta ccttcctaat ggcatgtctg aattgactgc
      421 aatgagactg aactgagttc ctttataaca gactccgcta atattctttc cgagtccata
      481 aaataccagc gtcaaatttt ctatcttttg taccaattta aaaatatcta tcggcgattc
      541 accatcagct cccaactttt ttctaagatt tggagcttta ttacttaaat ccattcgtga
      601 tattcctttc acctttttcc attttggcaa tcatgctttg attcaaagac aaatactcag
      661 caatcttatc ttgagtaaga tttgaagata ttctaagttc ttttattctt tttccaacat
      721 cacatacatt tatcatattc atacacctct cataaaaaga atagcacact cttgtcataa
      781 ttttttaaat aaaaaaatta tgacaaaaca aggaagcaat ttattgatgc tgcttaaaaa
      841 tctaaaattg atgagttcct atcaaaaatc gtatcttcaa cctcaaaaca gtacttaaag
      901 ctatccgact cggtttacat tgtcaaattt agattttatt tgagcataac tttctagttt
      961 gctttctgat ttttgtttaa tatagtagca aacattgaag gaaatatctc cacaagaaaa
     1021 cgcatactat taagcttttt caagacctaa taatatgcgc tgttctgatt tgaaagacat
     1081 tccattatta ttt
//
LOCUS       DAVMEF010000284         1083 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVMEF010000284 DAVMEF010000000
VERSION     DAVMEF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.285"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            337..>1083
                     /locus_tag="VYQ30_002042"
     CDS             337..>1083
                     /locus_tag="VYQ30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1967081.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDS"
BASE COUNT          315 a          187 c          249 g          332 t
ORIGIN      
        1 ttctaacagt tttatatcag aataataaat aaaaaagcga tattctagac tgtatcaatc
       61 cttttccatt ataggagcaa cagagtggga gtagtcatct aaggactaat ttatgtattt
      121 ttacgagtca gtatctttgg atactggttt ttacttttct agactttttg actacttgtt
      181 aaaactggga taattttcga ctgtttaaca gttattatgc aaagtctaaa agattagaat
      241 tgtcaaaaca atccgtctag gcttgatttt atcctttatt tactataaaa tgagaaggaa
      301 aaatgtcaaa cttttatatt gcaaatagga gaaatcatga caaaaacatt aaaacgtcct
      361 gaggttttat cacctgcagg gactttagag aagctaaagg tagctgttca gtatggagca
      421 gatgctgtct ttatcggtgg tcaggcctat ggtcttcgta gccgtgcggg aaactttact
      481 ttcgaacaga tggaagaagg cgtgcagttt gcggccaagt atggtgccaa ggtctatgta
      541 gcggctaata tggttatgca cgaaggaaat gaagctggtg ctggtgagtg gttccgtaaa
      601 ctgcgtgata ttgggattgc agcagttatc gtatctgacc cagccttgat tatgattgca
      661 gcgactgaag caccaggcct tgaaatccac ctttctaccc aagccagtgc cactaactat
      721 gaaacccttg agttctggaa agagctaggc ttgactcgtg tcgttttagc gcgtgaggtt
      781 tcaatggaag aattagctga aatccgcaaa cgtacagatg ttgaaattga agcctttgtc
      841 catggagcta tgtgtatttc atactctgga cgttgtactc tttcaaacca catgagtatg
      901 cgtgatgcca accgtggtgg atgttctcag tcatgccgtt ggaaatacga cctttacgat
      961 atgccatttg ggaaagaacg taagagtttg cagggtgaga ttccagaaga attttcaatg
     1021 tcagccgttg acatgtctat gattgaccac attccagata tgattgaaaa tggtgtggac
     1081 agt
//
LOCUS       DAVMEF010000285         1083 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVMEF010000285 DAVMEF010000000
VERSION     DAVMEF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.286"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            6..>1083
                     /locus_tag="VYQ30_002043"
     CDS             6..>1083
                     /locus_tag="VYQ30_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967082.1"
                     /translation="MLCFGLLAGVKADNRVQMRTTINNESPLLLSPLYGNDNGNGLWW
                     GNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDTKELREWYVKMLEEAQSLNI
                     PVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENYWIYNNQLAPHSAKYLEVCA
                     KYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLATKNTPIRDDAGTDSIVSGF
                     WLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYASEPESMIAMEMMNVYTGGG
                     TVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAPSKEEVVNRTKAVFWNGEGR
                     ISSLNGFYQGLYSNDETMPLYNNGR"
BASE COUNT          361 a          148 c          252 g          322 t
ORIGIN      
        1 ttgggatgtt atgttttgga ttgttagcag gagttaaggc tgataatcgt gttcaaatga
       61 gaacgacgat taataatgaa tcgccattgt tgctttctcc gttgtatggc aatgataatg
      121 gtaacggatt atggtggggg aacacattga agggagcatg ggaagctatt cctgaagatg
      181 taaagccata tgcagcgatt gaacttcatc ctgcaaaagt ctgtaaacca acaagttgta
      241 ttccacgaga tacgaaagaa ttgagagaat ggtatgtcaa gatgttggag gaagctcaaa
      301 gtctaaacat tccagttttc ttggttatta tgtcggctgg agagcgtaat acagttcctc
      361 cagagtggtt agatgaacaa ttccaaaagt atagtgtgtt aaaaggtgtt ttaaatattg
      421 agaattattg gatttacaat aaccagttag ctccgcatag tgctaaatat ttggaagttt
      481 gtgccaaata tggagcgcat tttatctggc atgatcatga aaaatggttc tgggaaacta
      541 ttatgaatga tccgacattc tttgaagcga gtcaaaaata tcataaaaat ttggtgttgg
      601 caactaaaaa tacgccaata agagatgatg cgggtacaga ttctatcgtt agtggatttt
      661 ggttgagtgg cttatgtgat aactggggct catcaacaga tacatggaaa tggtgggaaa
      721 aacattatac aaacacattt gaaactggaa gagctaggga tatgagatcc tatgcatcgg
      781 aaccagaatc aatgattgct atggaaatga tgaatgtata tactggggga ggcacagttt
      841 ataatttcga atgtgccgcg tatacattta tgacaaatga tgtaccaact ccagcattta
      901 ctaaaggtat tattcctttc tttagacatg ctatacaaaa tccagctcca agtaaggaag
      961 aagttgtaaa tagaacaaaa gctgtatttt ggaatggaga aggtaggatt agttcattaa
     1021 acggatttta tcaaggactt tattcgaatg atgaaacaat gcctttatat aataatggga
     1081 gat
//
LOCUS       DAVMEF010000286         1079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVMEF010000286 DAVMEF010000000
VERSION     DAVMEF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.287"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(719..979)
                     /locus_tag="VYQ30_002044"
     CDS             complement(719..979)
                     /locus_tag="VYQ30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967083.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
BASE COUNT          362 a          208 c          179 g          330 t
ORIGIN      
        1 tatatgcaca gacatatgaa atctttttga gatagcagtt atgatatatt tatccattca
       61 caacatcttc tgtaccaata ctacaatatt ggtacatttt gattacacaa tcgtttcaaa
      121 aaatatgatg gaagcctgag tattttatag taactccctt cctatcaact agatcaacca
      181 gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgagc agcatttcct
      241 agccaatagg acgctccaaa tcacctattc taaatccatc actagcttgg tcagtaagat
      301 acagactgca aggcaagact gacgtggttg atgagatttt ggaagagtat tacaatcata
      361 gattcaccag tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca
      421 cttctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt
      481 ctcgtttcat tctactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa
      541 aaacgaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttgaaa
      601 aaccttgata ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa
      661 gttttttcta gtttccagat atagacgaat tcaatatagc catctcgcta caattatttt
      721 aaccatcgag aaaatttttc agaatttcaa aaatttcatt ttgatccaat gaaatgtcat
      781 tattttgaag aatagttgac ttcacaggct ctaattctat cacaaagcgg tgcatgtctg
      841 cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg
      901 tcaaacgggt aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg
      961 tggcagagcg ctccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat
     1021 gaaaatcaaa gagcaaacta ggaagctagc tgcaggctgc tcaaaacact gttttgagg
//
LOCUS       DAVMEF010000287         1073 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVMEF010000287 DAVMEF010000000
VERSION     DAVMEF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.288"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            187..741
                     /locus_tag="VYQ30_002045"
     CDS             187..741
                     /locus_tag="VYQ30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967084.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLMIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEESKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            756..>1073
                     /locus_tag="VYQ30_002046"
     CDS             756..>1073
                     /locus_tag="VYQ30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967085.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFE"
BASE COUNT          401 a          140 c          204 g          328 t
ORIGIN      
        1 ggaagaatta tgttgaatca ggatctcttt gattcgcttg aggcacaaaa aattgtagat
       61 actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag agagacaatg
      121 atagtgtcga atggttatgc atggacgtga cctaatcata ttgatactgc atttgcatca
      181 gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata tttagaattt
      241 gaaacaaata cagaaaaata tgggaaagta ttgttaatga taaagggtaa gaagcgactt
      301 acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga atatgctcag
      361 atgaatacac tttatcttaa tcaacattct tcctacaaaa atgatgaaga tagtcattcc
      421 tttccaattc agatggagtt agtttctgat gaaatgattc aagaaattga acaagctact
      481 aaaaattcga atatcgaaaa atttatgatt ttaacttatg aggcggactc agaaaacaat
      541 attatatctg tagatgttgt tatgcctgat gcacgaactg gtcagttaca cttgattcaa
      601 gatttgtctg agtatattca atcaagttcg taccatttcg aagaatctaa atatcaagat
      661 attcctaatt tttcagaatt atcggaaaca gaagattttg aaattattcc aagaatagaa
      721 aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg gtattgaaag
      781 aattacggct gttaaatggt ttaagtagag cagaattagc tcagagaatt aatttaacgg
      841 aacaagccat ttggcagttt gagtccaacg aaacgaaacc taaattatca accaaaatgc
      901 atttggccaa ccaatttcat gttgatttaa cttattttga acaggaagaa gagagcattc
      961 gatttgattc ttctgtaatt gcctttagaa atgcagacct agcaacacgg aaaacaatag
     1021 atattcaaac tatgtattta cataaggtag atagtttgat tgattatttt gaa
//
LOCUS       DAVMEF010000288         1072 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVMEF010000288 DAVMEF010000000
VERSION     DAVMEF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.289"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..142)
                     /locus_tag="VYQ30_002047"
     CDS             complement(<1..142)
                     /locus_tag="VYQ30_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1967086.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGL"
     gene            complement(154..720)
                     /gene="amaP"
                     /locus_tag="VYQ30_002048"
     CDS             complement(154..720)
                     /gene="amaP"
                     /locus_tag="VYQ30_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1967087.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(804..968)
                     /locus_tag="VYQ30_002049"
     CDS             complement(804..968)
                     /locus_tag="VYQ30_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1967088.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          363 a          214 c          152 g          343 t
ORIGIN      
        1 ataatccagc tgcaactccc agtgctccta aaatcaatac aaatattgtt ttgaagaagc
       61 caaaggagac aatcaaacaa gccagaaata cgcctatgag accagcgata attggatatc
      121 gatattgttt aagccattcc attttttact tccttacttc acacgactaa cagtcttttt
      181 gttttgaggt tttggttggt aattttttac tgcaacttca agttttactt gacgctcaat
      241 accaaaaaac tgcttcaatc cattagttat ttcattttga attatttggc atctgtcagc
      301 gatgttgtct gaaggaagaa ttttaccttc tacatgaacg aaacatttat tttttcgtaa
      361 atttacatga acagttgggt tcttgatcaa tctatgatca ctcaccaaac ttctaacaaa
      421 accttcgatt gccgaattct ttaattttaa tgtatcgttt ttagtttcaa gttggatttc
      481 caagtaacgt ttaggataaa acatcacaac taaaatggat aataaaacta aagttgatag
      541 aaccactgtc ccccaaaaga catatctagc aagatagaat tcaactacgg agttctgtct
      601 ccagctaagt agatgaatac ctagatcact aacttgatga taatctatca aaacaggaag
      661 gaaaattgtc aagattaaaa tacagaaaat aatgaaacat attttctttg cttttgacat
      721 atacaatcct ttcgatttaa catttaaact atttttcccg tcattgattt aaaaatagtt
      781 tgctaacttt actggcgaaa agtttacttt ctacctgtaa agaatgacac tacagtaatc
      841 acaatcgctg cacctacaat agatgggagc aaagccattc cagcgattga tggaccccaa
      901 ctacctaaaa gagattgtcc tgcatatgcc ccgactaaac cagcgaatac atttgcgatg
      961 attcccatag aactactttt ttagtgattt tacctgccaa gagaccaata agacctccag
     1021 caataattga tcacaacact gcctaccctc cttttttatt ttaatatcaa aa
//
LOCUS       DAVMEF010000289         1056 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.290, whole genome shotgun sequence.
ACCESSION   DAVMEF010000289 DAVMEF010000000
VERSION     DAVMEF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.290"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(169..972)
                     /locus_tag="VYQ30_002050"
     CDS             complement(169..972)
                     /locus_tag="VYQ30_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1967089.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKDEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          343 a          212 c          179 g          322 t
ORIGIN      
        1 atcgctctga acatcaaaaa agaaaggacg aaatttgtcc tttctcaacc ttagcttttc
       61 ttcaacccac tacaattgac aaagagccaa actttgatga tatacgtttt cttgtgagaa
      121 tatttacttc atttttgcct aaaattcaat gtttactcag tatttggatt atgaaaaatc
      181 gaggtctaaa tctagataca ttttttctga agacaaatca ttttgaccac cgagcaagag
      241 attttcaaaa aaagctgtta aaaactcagg acgtcgctgt aaaatctttg cattatctaa
      301 taccaaggca tcacgaaaat acttggaatg ttgctgaaat ggtgtattat caatatcaaa
      361 accaaactca cgaagatact gaatcaaaaa gaccgttact gtccgagtgt ttccttcgcg
      421 aaatggatga atctgccaga ttcctgaaat aaaatgctgg atttgtttaa ccacatccgc
      481 ctgagttagt gtcgcatatg caacttgttt ttcctgatta aaatcataat ctaaggtcat
      541 ttgaatcatg gagtaatcag agtacacaac actttcacca ttcaaaacag gttcatcctt
      601 tgtgatattg gtctgacgaa attgccccac cggaatagag ggttcaaata tatcttgaaa
      661 caactcctta tgaatagcaa gtaaggtcgc aggactaaag ctaaagcctc ttcgagacaa
      721 tagttctaca atacgtagag aaaccaagtc tgcctcttcc gtacttgcat caatagtatg
      781 gtgataagcc gttgcatcct cataaacctg ctcataagtc agttctcccc gggactgttt
      841 ctcagccaaa gattccatat acgctgatgg cactagattg tcaactttct gcagaccaaa
      901 acctatccgc cataaatcac gcttcgcttc ataagacaag tttggattgt caatgttgta
      961 agttgattgc atagaaatat cctttcaatt gtttttctgt gtcatcggta aattgtgaaa
     1021 taggtatgga attggcgaaa attataaaaa tccttc
//
LOCUS       DAVMEF010000290         1036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.291, whole genome shotgun sequence.
ACCESSION   DAVMEF010000290 DAVMEF010000000
VERSION     DAVMEF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.291"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            128..>1036
                     /gene="pspC"
                     /locus_tag="VYQ30_002051"
     CDS             128..>1036
                     /gene="pspC"
                     /locus_tag="VYQ30_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEV1967090.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSP"
BASE COUNT          406 a          187 c          202 g          241 t
ORIGIN      
        1 ctccataata tccatagggg atttacccac tacaaatatt atagagccaa ataatgtagc
       61 aaatttcata tacaattcat attagcagga tatactgtag ttaattaaaa ttatggagaa
      121 tatagacatg tttaaatcaa atcatgagcg aagaatgcgt tattccattc gtaaatttag
      181 tgtaggagta gctagcgtag ctgttgccag tctttttatg ggaagtgttg tacatgcgac
      241 agagaaagag ggaagtaccc aagcagccac ttcttttaat aggggaaatg gaagtcaggc
      301 agaacaacgt ggagaactcg atttagaacg agataaggca atgaaagcgg tcagtgaata
      361 tgtaggaaaa atggtgagag atgcctatgt aaaatcagat agaaaacgac ataaaaatac
      421 tgtagctcta gttaaccagt tgggaaacat taagaacagg tatttgaatg aaatagttca
      481 ttcaacctca aaaagccaac tacaggaact gatgatgaag agtcaatcag aagtagatga
      541 agctgtgtct aaatttgaaa aggactcatt ttcttcgtca agttcaggat cctccactaa
      601 accagaaact ccgcagccgg aaaatccaga gcatcaaaaa ccaacaactc cagctccgga
      661 taccaaacca agccctcaac cagaaggcaa gaaaccaagc gtaccagaca ttaatcagga
      721 aaaagaaaaa gctaagcttg ctgtagcaac ctacatgagc aagattttag atgatataca
      781 aaaacatcat ctgcagaaag aaaaacatcg tcagattgtt gctcttatta aggagcttga
      841 tgagcttaaa aagcaagctc tttctgaaat tgataatgta aataccaaag tagaaattga
      901 aaatacagtc cacaagatat ttgcagacat ggatgcagtt gtgactaaat tcaaaaaagg
      961 cttaactcag gacacaccaa aagaaccagg taacaaaaaa ccatctgctc caaaaccagg
     1021 tatgcaacca agtcct
//
LOCUS       DAVMEF010000291         1031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVMEF010000291 DAVMEF010000000
VERSION     DAVMEF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.292"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(309..998)
                     /locus_tag="VYQ30_002052"
                     /pseudo
     CDS             complement(309..998)
                     /locus_tag="VYQ30_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
BASE COUNT          261 a          334 c          232 g          204 t
ORIGIN      
        1 gcatcaacaa gtgcttcagc ttcagcatca accagtgcct cagcttcagc aagtatctca
       61 gcgtctgaat cggcctcaac aagcgcatcg gcttcagcaa gcacaagtgc gtcagcctca
      121 gcaagtatct cagcgtctga atcggcctca acgagtgcgt ccgcttcagc aagtaccagt
      181 gcctcggctt cagcgtcgac aagtgcctct gaatcggcat caacgagtgc ttcagcttca
      241 gcaagtacca gtgcatctga gtcagcatca acgagtgcgt cagcctcagc aagcacatca
      301 gcttctgaat ctgcatcaac cagtgcgtca gcctcagcaa gtaccagtgc ctcggcttca
      361 gcgtcgacaa gtgcctctga atcggcctca acgagtgcgt cagcttccgc atcaacgagt
      421 gcgtctgagt cagcatcaac gagtgcgtca gcctcagcaa gtatctcagc gtctgaatcg
      481 gcatcaacga gtgcgtcagc ctcagcaagc accagtgcct ctgaatccgc atcaacaagt
      541 gcgtcggcct cagcaagtac tagcgcctca gcctcagcat cagcatcgac aagtgcatca
      601 gcttcagcaa gtactagtgc atcagcttca gcaagtacaa gtgcctcagc ttcagcaaac
      661 acaagtgctt cagtctcagc gtcaaccagt gcctctgaat ccgcatcaac cagtgcatca
      721 gcttccgcat cgacaagcgc atcggcttca gcaagtacta gcgcctcagc ttcagcaagt
      781 accagtgcct cagcttcagc aagcacaagc gcctcagctt cagcatcaac cagtgcatca
      841 gcttcagcgt caaccagtgc atcggcttca gcgtcaacca gtgcgtcagc ttcagcaagc
      901 acaagcgcgt cagcctcagc atcaaccagt gcctcggctt cagcaagcac atcagcatct
      961 gaatcagcgt caaccagtgc gtcggcttca gcaagcacct cagcatctga atcagcgtca
     1021 acaagtgctt c
//
LOCUS       DAVMEF010000292         1031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVMEF010000292 DAVMEF010000000
VERSION     DAVMEF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.293"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(63..302)
                     /locus_tag="VYQ30_002053"
     CDS             complement(63..302)
                     /locus_tag="VYQ30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1967091.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(322..594)
                     /gene="rpsP"
                     /locus_tag="VYQ30_002054"
     CDS             complement(322..594)
                     /gene="rpsP"
                     /locus_tag="VYQ30_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1967092.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            764..961
                     /locus_tag="VYQ30_002055"
     CDS             764..961
                     /locus_tag="VYQ30_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967093.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
BASE COUNT          317 a          190 c          175 g          349 t
ORIGIN      
        1 ttatttcatt ttactatatc ttctattcca cacaaaaaag cgagacatcc gtcccgccct
       61 tcttattttt cgtcaataac gattcttact tttttgtatt cagttgggac agagtagaca
      121 atcgttctta tcgcagaaat agtgcgaccc ttacgaccga ttacacgacc cacatcgctt
      181 tgatcaagat tcaaatgata ttccaaaaat tctggtgtat cctcaatctt gatagttaag
      241 gcatctggtt gtgaaattaa gggtttcaca atcgcaataa tgagattttc aatcgtatcc
      301 atctgtcaac ctactttaaa cttattttga gaatttagaa tcgtggaatt ttttcaatac
      361 gccttctttt gaaaggatgt tacgtactgt gtctgaaggt tgagctccat tagccaacca
      421 tgcaagaacg cggtcttctt tcaaagttac ttggttttca gcaacaagtg ggttgtaagt
      481 tccaactgtt tcgatgaaac gtccgtcacg tggtgaacgt gaatctgcta cgttgatacg
      541 gtagaaaggt tttttcttag aacccatacg agtcaaacgg attttaactg ccatttttaa
      601 agtctcattt ctttaatttt ttatttcggt gaaatagctg agctatttag cacatgttct
      661 attatagcag atttctggca ggtgtcaaga aaaaaacttg acagactata aatttcttaa
      721 agcatttaga aatttgttag aataggaaat aaatgtttga aagatgctat ccgtgaatac
      781 tattttagaa aaattttata aagaacacca agtcaaaccc ttcatatcgc ctgaacgaga
      841 gttggatact tggcttctaa gtcccaagcg aaatatggac ttattagcag atgattcact
      901 agcaggagat atcattttac tatggagaat ccagtttggt acttttacca ctgaaacatg
      961 attttaaaaa ctcccattcg aatatgattc aaaattcgct tagaatcaat gttttcccac
     1021 tttttcaaaa a
//
LOCUS       DAVMEF010000293         1010 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVMEF010000293 DAVMEF010000000
VERSION     DAVMEF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.294"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..798)
                     /locus_tag="VYQ30_002056"
     CDS             complement(<1..798)
                     /locus_tag="VYQ30_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1967094.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKE"
BASE COUNT          323 a          139 c          179 g          369 t
ORIGIN      
        1 ttctttggtt agtcgtaata aagttattcc tttatcaatc aaatcggagt tatgttgtag
       61 tattcccaag cgtacttgag agaatccaag aatatcgtat atttttaatt cttctgctaa
      121 taccaaaagt tgtagggtga ttgtttcaca ttctttgaat aaatgatgtt gaaaatatac
      181 agtggagaga ttggctaaaa aagatgataa aaatgctttt gtttctcgga agtatttata
      241 tttctcgatg gttttcataa ttctatcaat aagaagggga agattttcag aaggaaagaa
      301 aaagagaatg gttccaagca atttcaaatc actaatgtac catgtatcca taggttcaag
      361 atagtcccaa atttcttcat acaaatcttt caagacgtgg ttgtttttga atcctttagt
      421 tcgtaactct gtgacaattt ttgtatgacg atagatattt tgaataggaa catcgtgatg
      481 tgtcttaaga tatttttgac atttctcagt gagttcaacc atttttctag tatcttgaaa
      541 agtagacgga ttttggctct ctacaataat atctcgcctt ttactaggat ggtattcgtt
      601 gcatatatac ttgaattctg ccaagctcat atcaatttgt tcaagaagaa ataccatgtt
      661 ttcgaaactg ggaataactt taccgtgttc aatatttgtt agagttgttc gatgtatgac
      721 ttgtccacat acatcttgtt gggtcaatcc ttttgacttt cgaatttctt taaaaatttg
      781 tccataatcc catctcatat tgttcctcct agaaaaatgt gaatattttc tacatttata
      841 aaaaacgtag aaaaaggtgg tattattata tcaaaaagga gggaaaaagt catgggcttt
      901 aaaaaatatt tgaagaattt accgaaaaac tctggatttt tgatttggag ttggattcaa
      961 cttatctggt ttgaaacatg gttttgggga taaagaaatt agagtctata
//
LOCUS       DAVMEF010000294         1005 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.295, whole genome shotgun sequence.
ACCESSION   DAVMEF010000294 DAVMEF010000000
VERSION     DAVMEF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.295"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            284..523
                     /locus_tag="VYQ30_002057"
     CDS             284..523
                     /locus_tag="VYQ30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967095.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            508..786
                     /locus_tag="VYQ30_002058"
     CDS             508..786
                     /locus_tag="VYQ30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967096.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKLTSYCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
BASE COUNT          352 a          139 c          181 g          333 t
ORIGIN      
        1 gttaaaatat ttctagaaat taatttgact ttcctaatcg atttgttcac atcttgtttc
       61 aatctactat actttctcgt taaaccgttc ggcaattcat ggtgacatgg acacatagta
      121 gtaagcacac tatgtggctt ggcagagcta aaaactgttc ccttgcgata agcctaataa
      181 gcacaacata gggagttaga gaagccgact ctaatcaacc actttgggca gtagtgagaa
      241 ctgccccgtg cttttttatt ttgagaaaat atggagtttg tcgttgaaat tacttgattg
      301 tattttggac tatcaagaaa gattcaatgg aaaaacatgt caagtatcaa cgaattataa
      361 gtatttagag attttcaaag tgaatttttg cttgactgat ttacatcatt tatttgactt
      421 atacaaaatc acacgagatt atgctagtca aacaaaacct gctattcaag ctggtgtttt
      481 tattttagaa gattttagaa atatcctatg tacaatgatg taatcgaaag gatatcttta
      541 tatgagttta tcggcgatat cttctattct aagctaacaa gttattgcat cgtagctaag
      601 gatttatcta aaaatactat gaaattggac gtcatatttt ttgaggataa aaataaaaga
      661 tccgcagttt taggtttacg aagagacaaa agcggagtat ttaaaccagt tactctacat
      721 tttacaagcg ctaagaaata tgctaaagtt cgtaaaacag atgtgaaaga aatgaaatgg
      781 ctataaagat tgtccgcagc cttaagcttg cgttttttgt tgtttaacga aattaaaaaa
      841 caagctctct catcggaaat gtttttatag gaatgaagga agatgatgct atgtttaaaa
      901 agagaatcga aaagggcaaa agtagtgtct ttatcttttt ggagtagaag cttatgatat
      961 agaaatttgt aataaaaatg taatataaaa catttgacaa atatt
//
LOCUS       DAVMEF010000295         1000 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.296, whole genome shotgun sequence.
ACCESSION   DAVMEF010000295 DAVMEF010000000
VERSION     DAVMEF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.296"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            64..252
                     /locus_tag="VYQ30_002059"
     CDS             64..252
                     /locus_tag="VYQ30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1967097.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            314..>1000
                     /locus_tag="VYQ30_002060"
     CDS             314..>1000
                     /locus_tag="VYQ30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1967098.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRAR"
BASE COUNT          340 a          123 c          216 g          321 t
ORIGIN      
        1 tattataaaa gccagttgac acaagtgtta cacctgtgat agaattaact tgaggtgatg
       61 aaaatggcaa ccgataaaaa tagaataatg attagcttgg atgataaaaa tctagagaaa
      121 cttgaaaatt tagtagaaga tgctagagat agaaggggaa tgcgactaac aaagtcccag
      181 gttattgaat tactcctaaa taccgttgat tatttcgatg atattatggg ggctatttac
      241 tcaaaaaaat aagagcaaaa ttgctattat tttttacaaa ttggtgtaac actagtgata
      301 taggagtgat gaaatggttg tttatattag acaaagtaaa ttaccaagtg aggtttcaat
      361 taataaatac catgctcaag taggtgccta cttacaagga gaagaagctg ttttatatca
      421 atctttttct gaaataaaag agttaacaag tgaagatata gttgtagatt atataatgga
      481 gactagagca ttactaaaaa tgatgggctt aaacgttccg gttcatgact atcctattga
      541 gcttaaagag ttttatggtc gaaagattta tgctggtatt ttaggagaga ttgtgaatat
      601 acctgataat tggggaaaat ttattaagcc taaagctggt tcaaaagtct ttactggaag
      661 agttgttaat ggaacccatg atttaatagg tattggtcta cctttcgact atcctatatg
      721 gattagtgag gttgtagaat tcatagctga atggcgttgt tttgtgttag atggtcgcgt
      781 attagatgtt cgaccctata caggtgatta tcatgcacaa tttgatgcaa gtgtaattga
      841 tgaagccata tcatgttgga aagatgcgcc aatagcttat ggactagata tcggtgttac
      901 tcgcgatggc agaacacttg ttgttgaagt aaatgatggt tatgcattgg gaaattatgg
      961 cctatctcct ttaaaatcaa tcaattttca tagagctagg
//
LOCUS       DAVMEF010000296          982 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.297, whole genome shotgun sequence.
ACCESSION   DAVMEF010000296 DAVMEF010000000
VERSION     DAVMEF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.297"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            590..964
                     /locus_tag="VYQ30_002061"
     CDS             590..964
                     /locus_tag="VYQ30_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1967099.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          343 a          122 c          195 g          322 t
ORIGIN      
        1 aatctatatt tacggcatta taatgaaaaa aataaatatg aatcaatgtt aaagaaaatt
       61 ttagaggtaa gaccagactt tgttttaatc gagcagaata ttgatgatag tttaaatagc
      121 tcgcaaccat tatctttatc tttagccatt tcagaaatcc aacttttgct tgaagtgtat
      181 atggggatca aacatgtttc aattaggaga taaaaccaga ataatatttg ccattatagg
      241 ggcaagttgc atattagtca gtatgtatct tattccactt attaaaactg agaataaatt
      301 ttgggaaata cttttattta tattaggtgt attgctaatt ggaattgcgt tattaagtca
      361 aaaaaaataa attttgaaat agtattagtg agttcgcaat catttattgc tagtggttta
      421 gaactgttaa gtttcaaaca ataattgatt ttaaacctta ggtaacctat aaaaacaaat
      481 aaggtagaga aaggatatct tatctaggta gctaagtgat aaaaacacag tcgtctatca
      541 cttgttagga aactatccta actatgctta ataaaaaagg agaaaataaa tggaactcgt
      601 attaccaaat aattatgttg ttcttgagca agaagagatg atgtatcttg atgggggatt
      661 ttctattccg agatggcctg ttgcaacagc cattaatata gcttttaatg gtgttttagg
      721 tggaggagca atcagtctag ttagaaatta tattcgtaat tatggtttgc ggcgagttac
      781 aagcgcaatt gctggagcag ctgcaagata tgttggggta cgagttgcaa atagagtggc
      841 aggatttgca ctgtctgcta ttaatggatt tgcagcttgg atgtcaattg gcaatgctat
      901 tacaacaatc tgggccaata atgatgtaaa taggagagac ccaaatttaa acgccttgtg
      961 gtaactagat atgggtatta aa
//
LOCUS       DAVMEF010000297          972 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.298, whole genome shotgun sequence.
ACCESSION   DAVMEF010000297 DAVMEF010000000
VERSION     DAVMEF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.298"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..916)
                     /locus_tag="VYQ30_002062"
     CDS             complement(<1..916)
                     /locus_tag="VYQ30_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967100.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEAKNICYKYGDKEILKNLN"
BASE COUNT          339 a          171 c          128 g          334 t
ORIGIN      
        1 aatttaagtt ttttaatatt tctttatctc catacttata gcaaatattt tttgcttcat
       61 ataacggaaa atctctattc acctcatttg gttcgatatc attcatttta tttgactcaa
      121 ttggattaat tgaatacaat tttaaaaaaa taggcttcgt accaataata gaggataatt
      181 gacctcctaa ttcacctagc gctgtaaaaa taacacctgt tagtgctcct attgcttcaa
      241 tagtaccaat tttcactatt ccttttattg caagatagcc tgttaaaaaa acgagagata
      301 tctgaaaaaa aatattgaga aagaagctaa tagcgcctgc taacgtttct acagttgtct
      361 ttctttgtat aaccatcttt aataaaattc ctgcttcttt aattttctta ggcaatacat
      421 ataaaagatt caaggacgct aacacatcaa atccattcaa tatagtctca ctagatttta
      481 aaaaagcttc attttggtta gttaaattta gactaacttc tcgcattttc gatgcaaaga
      541 tttttggtac aagtagcata atcattaatg aaaacaaggt ggctacagtc aatgaccaat
      601 gatagtgatt aagagtcaca actgcaaata tagtaccaga aattcctttt attactaaaa
      661 aaagttgttt aaaagcctga tcatttaaag tctgaacatc attatttagc cacgaaagat
      721 atgttcctga tgatttacta tgaaattctt gataggtaga gttagagatg tctgtggcaa
      781 ctctatttcg aatctctaga ttaaactctt ggatcacttc aacctgataa tttttcacta
      841 cccagtcaag gaatattatc ccacaccaga caatcatttg gtagattgac aatttcaaaa
      901 accgctctaa attcatcgca attaattcat tcaacaccag agcattaata gttgctgcat
      961 aaattagcaa ta
//
LOCUS       DAVMEF010000298          961 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.299, whole genome shotgun sequence.
ACCESSION   DAVMEF010000298 DAVMEF010000000
VERSION     DAVMEF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.299"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..237
                     /locus_tag="VYQ30_002063"
     CDS             <1..237
                     /locus_tag="VYQ30_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967101.1"
                     /translation="KKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSETL
                     EKGPESNASSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            282..911
                     /locus_tag="VYQ30_002064"
     CDS             282..911
                     /locus_tag="VYQ30_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1967102.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
BASE COUNT          321 a          124 c          207 g          309 t
ORIGIN      
        1 aagaaaactg tttataaaaa attgggtatt tcaattattg cgagtacttt attggctagc
       61 cagttatcga cagtatctgc tttgagtgtt atttctagta caggtgaaga atatgaggta
      121 agtgagacac tagaaaaagg tccagagtct aatgcttctt cattatctga gatttcacca
      181 acgtatggtt catactacca aaagcaatca gaagtattat cggtaatgat gatttgatta
      241 aaattgataa tactttacag tatccgtatt cgacttcagc tatggtttta tctaagtatt
      301 atggagtagc cgatggaatg aatgtagaag ggaggggaag tgcgaatttt attaaagata
      361 atgtgttaat tacagcggct cacaactact acagacatga ctatgggaaa gaagcggatg
      421 atatttatgt tcttccggct gttagtccaa gtcaagaact atttggaaag atcaaagtaa
      481 aggaagttcg ttatttgaag gaatttagaa atttaaattc taaggatgca agggaatatg
      541 acttggcttt attaattcta gaagagccca ttggtgcaaa attagggact ttgggtcttc
      601 ctactagtca aaaaaatttg acaggaataa ctgtgactat cacaggctat ccatcatata
      661 attttaaaat tcatcaaatg tatacagata aaaaacaagt tttaagtgat gatggcatgt
      721 tcttggatta ccaagttgat actttagagg ggtctagtgg atctacagtt tatgatgcta
      781 gtcaccgtgt agtaggagtg catactttag gagatggagc taatcaaatt aacagtgcag
      841 ttaaattaaa tgaacgaaat ttgccattta tttattcggt tcttaaaggt tactctcttg
      901 aaggatggtg aatgggttag gtaatgaagg ctaattgtaa actgtgatgg atacttaact
      961 t
//
LOCUS       DAVMEF010000299          953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.300, whole genome shotgun sequence.
ACCESSION   DAVMEF010000299 DAVMEF010000000
VERSION     DAVMEF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.300"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            91..>953
                     /locus_tag="VYQ30_002065"
     CDS             91..>953
                     /locus_tag="VYQ30_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEV1967103.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEK"
BASE COUNT          305 a          163 c          199 g          286 t
ORIGIN      
        1 aggttttttt cgattttttg gaaaaataat tgactaaaca aaccaaacgt tgtataataa
       61 aacaaatatt taaagcagga ggttctggaa atgaaaaagt ctaagagcaa atatctaacc
      121 ttggcaggtc ttgtcctggg tacaggagtt ttattgagcg cgtgtggaaa ttctagcact
      181 gcatcaaaaa cctataacta tgtttattca agtgatccat ctagtttgaa ctatctagca
      241 gaaaaccgcg cagcaacatc cgatattgtt gcaaatttgg tagacgggtt attagaaaat
      301 gaccaatatg ggaatattat tccatcatta gcagaggatt ggactgtttc tcaggacggt
      361 ttgacctata cctacaaact tcgtaaggat gccaagtggt ttacttctga gggagaagaa
      421 tatgcgcctg taactgccca ggattttgtg acaggtttgc aatatgcagc tgataaaaaa
      481 tcagaagctt tgtatctagt gcaggactct gttgctggtt tggatgacta tatcactggt
      541 aaaacaagcg acttttcaac tgtcggtgtc aaggcacttg atgaccaaac ggttcaatat
      601 actttggtta aaccagaact ttactggaat tcaaaaacac ttgcaacgat actttttcct
      661 gttaatgcag atttcctgaa atcaaaaggg gatgattttg ggaaggcgga tccatctagt
      721 attttgtaca atggaccttt cttgatgaaa gcacttgtct caaaatctgc tattgaatat
      781 aagaaaaacc ctaattactg ggatgctaag aatgtctttg tagacgatgt gaaattgacc
      841 tactatgatg gtagcgacca agaatcactg gaacgtaatt ttacagctgg tgcttatact
      901 acggctcgtc tttttcctaa cagctccagc tatgaaggga ttaaagaaaa ata
//
LOCUS       DAVMEF010000300          953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.301, whole genome shotgun sequence.
ACCESSION   DAVMEF010000300 DAVMEF010000000
VERSION     DAVMEF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.301"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..186)
                     /locus_tag="VYQ30_002066"
     CDS             complement(<1..186)
                     /locus_tag="VYQ30_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967104.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYC"
     gene            complement(186..950)
                     /locus_tag="VYQ30_002067"
     CDS             complement(186..950)
                     /locus_tag="VYQ30_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967105.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLVLGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
BASE COUNT          399 a          174 c          141 g          239 t
ORIGIN      
        1 gcaatatact ccagtcgtca tatcttgaag taaaactaaa atccattcta atacatttgg
       61 atggattgaa tattggcgac agcgcaataa atatactgta ctagataaaa cacaggatag
      121 cagtaatata aaataaacca atactgataa aaaatctttt ttgtaaaatt gaacaaattg
      181 tttcattata catagtcctc tgaatgtaga aaaaatgtac cataaacaac caaacaacta
      241 acaaataaaa taaaagcaag atgcccacta actaaggaaa gactgatatc tttctgatat
      301 cccaaaacta atgttgtcac aggttctaag taagatagcc ctaaaatagc ccaaaaaata
      361 ccaccaacca tcatataggc aactgggatg aaaatagctc ctattttttt cttcactagc
      421 aaagcactag ctagtccaaa aatagagaac acagcgcccc aaactccata ccagagagtc
      481 gtcacaagac tatagagcaa ctgattagaa tcaaataatt cttttaaggc accactataa
      541 tctccaatat aaatttcctg ataaggagtc actaaaagat taattcctaa taataataaa
      601 taggggagaa aaaagactag aaaagaagaa ataattgcag tactacctac aatagccaga
      661 tacttctttt tagaaattcg gcacaattgt gctgttagaa aatgactctc agcatcctct
      721 attatctgac tagaataggg cagtgtacag ataagtgcag ctactaggct aatcggtgaa
      781 aatcctcgaa tagaagaagc ggcaaaaaaa atcgataaac cttcaatttt ataaatacca
      841 ttgaaagcaa ggaaatttcc taaactcatg caaagaaggg tcaaaaataa agacatataa
      901 aatcgaggtg attgaacgac tcgtacaaga ttacaaatga aaaattccat cct
//
LOCUS       DAVMEF010000301          950 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.302, whole genome shotgun sequence.
ACCESSION   DAVMEF010000301 DAVMEF010000000
VERSION     DAVMEF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.302"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..737)
                     /gene="lytA"
                     /locus_tag="VYQ30_002068"
     CDS             complement(<1..737)
                     /gene="lytA"
                     /locus_tag="VYQ30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1967106.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIA"
BASE COUNT          248 a          234 c          178 g          290 t
ORIGIN      
        1 tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca gtaccagttg
       61 ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc aaagtagtac
      121 caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga atgtacgtac
      181 cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca
      241 tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg gtcaacgtgg
      301 tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa
      361 ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg
      421 atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc aatcagttca
      481 accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc attatcaaca
      541 ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc taattctggg
      601 tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt cccagttgag
      661 tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt tcttaattta
      721 ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt tttacaatcc
      781 agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc acgattaggc
      841 ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg acaaggtctg
      901 cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat
//
LOCUS       DAVMEF010000302          927 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.303, whole genome shotgun sequence.
ACCESSION   DAVMEF010000302 DAVMEF010000000
VERSION     DAVMEF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.303"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..401
                     /locus_tag="VYQ30_002069"
     CDS             <1..401
                     /locus_tag="VYQ30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEV1967107.1"
                     /translation="DKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIAD
                     DLVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRK
                     AIDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            622..>927
                     /locus_tag="VYQ30_002070"
     CDS             622..>927
                     /locus_tag="VYQ30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1967108.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     "
BASE COUNT          328 a          118 c          171 g          310 t
ORIGIN      
        1 gtgataaagt ttatgatgaa atatttgttg aagaggtagt tccgcacgtt tttctgccat
       61 atgaatctga cttacttctt attttaccag ctacggcaaa tgtgattggc aaaattgcta
      121 atggtattgc tgatgattta gttacagcaa ctgttttaaa ctttaataaa aaaataattt
      181 tttgtcccaa tatgaactct actatgtggg acaatcacat agttcaaaga aatgtatcaa
      241 ttctaaagga gttgggacat atatttttat ttgagtctaa aaaaacatat gaggtaggat
      301 tgcgtaaagc aatagattca acatgttcaa tgttacaacc acagtcgtta gtaaaagaac
      361 ttatcaaatt agaaaatatt gtccttgaag agggacatta aaaactactg agaatattaa
      421 ggaggggaaa aaatggaaaa ttcatcaatc gatgtagata tgctgttgga agaattgaca
      481 caagaagcaa tggtcgttgt tgctgttgat aaggactgtt aatttaaact tatggcaata
      541 tatgaaaggt tactggatgt tttaaattat gcaggcagta gccttttatt atatacaaat
      601 ggataaagta aggataatac aatgattaat aaaaaaatac aacaagttgt tttggaatca
      661 ttacagaatt ttttgaatgg gaacttcatt tcgccttgtg tagtctatga ttttggcttg
      721 ctggaaactg tacttgatga atttaaaaat caaattcctg taacattcaa ttaccaactt
      781 ttttatgccg ttaaagcaaa ttcaaatgag aagatacttg aattcttagt agataaaatt
      841 gatggagttg atgtggcgtc attatctgaa ttagatgtgg ctaaaaaatt tttcccacca
      901 actcaaattt ctgttaatgg tcccgca
//
LOCUS       DAVMEF010000303          926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.304, whole genome shotgun sequence.
ACCESSION   DAVMEF010000303 DAVMEF010000000
VERSION     DAVMEF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.304"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(86..235)
                     /gene="blpO"
                     /locus_tag="VYQ30_002071"
     CDS             complement(86..235)
                     /gene="blpO"
                     /locus_tag="VYQ30_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1967109.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(479..682)
                     /gene="blpN"
                     /locus_tag="VYQ30_002072"
     CDS             complement(479..682)
                     /gene="blpN"
                     /locus_tag="VYQ30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1967110.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(698..919)
                     /locus_tag="VYQ30_002073"
     CDS             complement(698..919)
                     /locus_tag="VYQ30_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1967111.1"
                     /translation="MDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLGTAICAASGVG
                     APFMGACGYIGAKFGVDLWAGVTGATGGF"
BASE COUNT          308 a          236 c          113 g          269 t
ORIGIN      
        1 gctacatagg ctacacctcc aagtatagct ccacctgcag caccagttac acctattcct
       61 atagcaaatg gtcccaatag aaatgtcaaa ccgttgttgc acacccatca attgcgccat
      121 atgcaacccc tgctgcacaa ctaatttttc ttccccaatc aatatctcca ccttcaacgc
      181 aagcaagcat ttcattatcc ataacagaaa attgtgacat catttttgta ttcatatagt
      241 atgtcacttt tcagttacgg aacaagttta atataaaaat tatcaaaaaa acataggcaa
      301 taaagagaaa aattaattta tcatagatta gaaataatat gacaaaacaa ttcaatgatg
      361 ttaattcaat agtcttttgt tttttatcag agatacttat ggatagataa ataagatagg
      421 tttgaaaagc gaagagaata ataaagaata tagccttcat aaaatttagc tttcattttt
      481 atgatgtagc ggtataggct aaatatccac aaaccactgc tcctccaatt cctcctattg
      541 cagcgcccca tggtcctaga attttcccat atttcactcc acccgctgca caacctaaag
      601 cagcaactac agctgctcct ccggaattac ctccataaac ctcactcagc attgtttcat
      661 ttatattaca ataagtattc atacaagtct ccttttatta aaatccaccc gttgcccctg
      721 ttactcctgc ccaaagatcc acaccaaatt tagctcctat gtatccacat gctcccataa
      781 atggtgctcc aacaccactc gcagcacaaa tagctgtccc tagcccccag ccaccaaaag
      841 cagcaccacc accttctaag acattagttt gccaattatt cttgcctcct tcaatactag
      901 ataacatagt tatatccatt tcatga
//
LOCUS       DAVMEF010000304          918 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.305, whole genome shotgun sequence.
ACCESSION   DAVMEF010000304 DAVMEF010000000
VERSION     DAVMEF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.305"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            41..757
                     /locus_tag="VYQ30_002074"
     CDS             41..757
                     /locus_tag="VYQ30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1967112.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT          324 a          183 c          149 g          262 t
ORIGIN      
        1 ggtaaaattt tctatggaga aggaaaatag aggtaaaaat atggcaattc ccaaatatca
       61 atacataaaa gacgaattaa aaaataaaat catctctggg cagtttagca gtggagataa
      121 attttacact gaagcagaac tcatttcaat gtacgatgtc agctctatca cagttgttcg
      181 cgccttaaac gaccttgcca aagatggcta tattgtccgc caacaaggaa aaggaacctt
      241 cgtttcacgt gcacgcaaac acaagcttgt agagttttca gatattgaac tcttcaatgc
      301 taaagatgat aaggtaactg ttctttcaat tgaacgcgga aacaagcttg tttacttgga
      361 aaaattgggt ctacgcggcg atcaattcta ctacaagatt gaacgtatcc gcgaatcaaa
      421 cggtgtcgtt tatatctacc atacatccta catcccagaa caatatatca acgcaaatta
      481 tccaaacctt gaatactata gttctatcta taatcgtttc aagttagact accacattca
      541 catgaatgat gaacactttg aagaaatcaa cgaaatagtc tttccaactc cagaacatgc
      601 ggcttcagtc ctcggagtcg atgaacaatt ccctactgtc ctacaaacta aaatcaccaa
      661 actcgaatca actggccaag ttttagaata cagtgaaact tacaaacgat ctgattacta
      721 caagattaaa tttatctcat gtgaccgtga tcactaagaa gaaagcctga gcctaatcgc
      781 tcgggctttt tctatgctta ttataccaca ccaaaaaggt agcgcttacc tatttttgga
      841 agattctata atttgtggaa tattttatag aaatagaacc aaaaattata ttagatatat
      901 atatgcattt tttccgac
//
LOCUS       DAVMEF010000305          916 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.306, whole genome shotgun sequence.
ACCESSION   DAVMEF010000305 DAVMEF010000000
VERSION     DAVMEF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.306"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>916
                     /locus_tag="VYQ30_002075"
     CDS             <1..>916
                     /locus_tag="VYQ30_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV1967113.1"
                     /translation="EPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSS
                     MLECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEA
                     YRQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKV
                     SDSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDR
                     AVFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLC
                     PQNSDEDKKLYSQGTDNTRGVCSVAGSIL"
BASE COUNT          362 a          119 c          194 g          241 t
ORIGIN      
        1 tgaaccaata atatctaaag aagattttga aaaagctcag gagattagat tgcgtcgagc
       61 aggaaataag aagacagctg cgaatgtaaa tgggaagcgt gaacgctatt caaaaatgta
      121 tgcttttagt agcatgttgg aatgtggttt ttgtggttca atattatcta gaagaagttg
      181 gcactgccgt tcagattatc gcaaagttgt gtggcactgt gttacatcga taaagaaagg
      241 aaagaaattt tgtaagcata gtaaaggatt agaagagatc gccattgagg gggctttcct
      301 ggaagcttac agacaagtct atcattcaaa tgaaaattta atgacagact tacttgaaac
      361 gatagaatct gaattgaatg acaatagcct taacaaggaa ctaaaaagga ttacaaacaa
      421 acttcgaata ttactaaaaa aagaggagaa tcttgtaaat ctaaggctcg aaggaaaagt
      481 tagcgattcc atatacaatg aaaagtacaa tgaaatttcc tcagaaaaag agtttctagc
      541 agaagagaag gtaaatattg aaacaacatt aaaatcagaa atcgatgtaa agaaaagact
      601 aactgagttt aagcacttgc tatcttcgca aaaaatgctt acggagtttg atcgtgcagt
      661 ttttgaaagt attgttgaga aaattattgt aggtggagtt aatagcaacg gagaaattga
      721 tcctgcaatg ttaaccataa tattcaaaac aggtgaaatt caaaacaagg atggtaaaca
      781 atttaaaagt aaacgtaaaa atgctaaact agaaacagat aaattgtgtc ctcaaaacag
      841 tgacgaggat aaaaaattgt attctcaagg aacagacaac acacgtggag tgtgtagtgt
      901 tgctggttcg atctta
//
LOCUS       DAVMEF010000306          903 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.307, whole genome shotgun sequence.
ACCESSION   DAVMEF010000306 DAVMEF010000000
VERSION     DAVMEF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.307"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            329..517
                     /locus_tag="VYQ30_002076"
                     /pseudo
     CDS             329..517
                     /locus_tag="VYQ30_002076"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          347 a          169 c          125 g          262 t
ORIGIN      
        1 acatatagta tttagcgcca catagtactg aactctctcc aaaaacggtt atgcctcttt
       61 gaatagggcg ttatcacaag aaaagcatct ccacgtttca acttcatatg gctcaaaaac
      121 aatcaattga tgctaaaacc tgtatctaga tgtttcggtt cataaaacca tgaaactgta
      181 aaagtggatg aaattgatag cgatagtcaa atcaagaggc atcataactc taaaaagtca
      241 caatatataa gttcatcctc ggaaaaatat cattctaatt attgaaatgc ctacatgaaa
      301 agaaacgtta aatgctcatg aaacaacgaa tacaggtatc aaaactgtga caaaacaagt
      361 ccctaaattt actaaagaca ctgctcaact ttacacctgt aaatggttgt tgtataataa
      421 agttacaaag atgtacgacc acactgttgt aaatcatagt gttcgcgaat atattactga
      481 tagcatttct acaaatacaa ttgaagagat tggataggct ttaaacgaaa tatgtcagtg
      541 ctttggcatt cctagccttc atatcattta aagaattcta tagacaaaat tttttccaat
      601 acagacactc gtaacaactg cttcattttt ctaccaacat atttaggaac aggataagat
      661 acaagagtat taatccatag ctcagttcta taccaatcta agacaaataa gctaaaaaaa
      721 cgattgataa taagcaaata gattccaaat tttctctatc tgctcatttt aataaacaat
      781 actagtgtaa ctatccttcc agtcagaagc ttgtcaaatc acaccgaaaa ttcttctaaa
      841 atttatctcg ttaggcaatc aagcaaaaac tcgacgatag tacaaacatt atcatacagg
      901 att
//
LOCUS       DAVMEF010000307          902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.308, whole genome shotgun sequence.
ACCESSION   DAVMEF010000307 DAVMEF010000000
VERSION     DAVMEF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.308"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            44..>902
                     /locus_tag="VYQ30_002077"
     CDS             44..>902
                     /locus_tag="VYQ30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HEV1967114.1"
                     /translation="MRNIIKKYDEIINKVDSLVLDNNIIDLLQRSCYTENRSYLSEYP
                     SIIIYLSYRLANCDDNEHSKLLYNRVNYYLHELLKSIKLNSRNNISMCYGFSGYVYAL
                     KLLPKRSKEYSKLLETLETILVSLTRDRLSEIKKSNKVKEEYIDVIQGVSSVGKYFLS
                     KDKLTSNQELLLKGVLNYLAGVINNKPTIYPEYMPNEKLKRKFPNGYINLGVAHGILG
                     PLYVLALGFKKFNMPEYLISLKKGLSYYEKTFQTNKIGKIIGWNGRVSAEVESEKFEY
                     NLSWCYGSLG"
BASE COUNT          343 a           99 c          167 g          293 t
ORIGIN      
        1 tatctttatt ttgtgaggta tattttagag gtattaaata attatgagaa atataattaa
       61 gaagtatgat gaaatcatca ataaagtaga tagtctagtg ttagacaata atattattga
      121 tttactacag cgctcgtgct atacggaaaa tagaagttac ttgtcggaat accccagcat
      181 aatcatatat ttatcatata gattagctaa ttgcgatgat aacgagcatt ctaagttgct
      241 ctacaataga gtaaactact atttacatga actgttaaag tcgattaagt tgaacagtcg
      301 taataatata tcaatgtgct atggattttc tggctatgta tatgccttga aattgttacc
      361 taagaggagt aaagaatata gtaaattgct agagacactt gaaacaattt tagtatcttt
      421 aactagagat aggctaagtg aaataaaaaa atccaataaa gttaaagaag aatatattga
      481 tgtaatccaa ggagtttcta gtgtaggaaa gtattttctt agcaaggata aattgacttc
      541 taatcaggaa ctattattaa aaggagtttt aaattattta gcaggagtaa taaataataa
      601 gccaactatt tatccggaat atatgccaaa tgaaaaatta aagagaaaat tccccaatgg
      661 ctatatcaac ctaggtgtag ctcatggtat attaggacca ctatatgttt tagcattagg
      721 gtttaaaaaa ttcaacatgc cagagtattt aatttcgcta aaaaagggtt tgagttacta
      781 tgaaaaaact tttcaaacta ataagattgg caagattatt ggctggaatg gtagagtaag
      841 tgctgaggta gaaagtgaga aattcgaata taatttaagt tggtgttatg gtagtcttgg
      901 aa
//
LOCUS       DAVMEF010000308          876 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.309, whole genome shotgun sequence.
ACCESSION   DAVMEF010000308 DAVMEF010000000
VERSION     DAVMEF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.309"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..876)
                     /locus_tag="VYQ30_002078"
                     /pseudo
     CDS             complement(1..876)
                     /locus_tag="VYQ30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          271 a          154 c          187 g          264 t
ORIGIN      
        1 ggttccttat tctggaagtg aaaaagcaag agctgataga gttgatagtg gtgtttcaag
       61 tcttccgaat aactcaaaag cttgtttaaa atctctttat tggttaagtg catacgaaaa
      121 gtaggacgat aaaaacgttt atcactcagt ttacggctat cctgttgaat gagtttccag
      181 tagcgcttga tagccttgta ttcatgggat tttcgatgaa actgattcat gatttgaaca
      241 cgcacacgac tcatagcacg gctaagatgt tgtacaatat gaaagcgatc aagaacgatt
      301 ttagcattcg ggagtgagac agtctgggag actgtttcag cctgagccta gaaatttgaa
      361 agcgaagctg tttagccaag tcatagtaag gactaaacat atccatagta ataattttga
      421 cgcgacatcg gacaactcta tcgtagcgaa gaaagtgatt tcgaatgata gcttgtgttc
      481 ttccctcaag aacagtgatg atattgagat tgttaaaatc ttgcgcaatg aagctcatct
      541 ccatctcccg attgaaacag tcactccccg gactgtttca acgtcccaag acataatctt
      601 aggcagattt ctaaaattac actcaaagtg gaagtcattg agctttcgaa tgacagttga
      661 agttgaaatg gccagctgat gggcaatatc ggtcatagaa atcttttcaa ttaacttttg
      721 cgcaatcttt tggttgataa tacgaggaat ttggtgattt ttcttgacga tagaagtttc
      781 agcgaccatc attttcaagc aatgatagca cttaaaacga cgttttctaa ggagaattct
      841 agtaggcata ccagtcgttt caaggtaagg aatttt
//
LOCUS       DAVMEF010000309          864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.310, whole genome shotgun sequence.
ACCESSION   DAVMEF010000309 DAVMEF010000000
VERSION     DAVMEF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.310"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(264..434)
                     /locus_tag="VYQ30_002079"
     CDS             complement(264..434)
                     /locus_tag="VYQ30_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967115.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(447..>864)
                     /locus_tag="VYQ30_002080"
     CDS             complement(447..>864)
                     /locus_tag="VYQ30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967116.1"
                     /translation="LKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGG
                     QRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHRLSTIKD
                     ADIIFVMKGGKIVESGNHKYLMTLGGEYYSLYTKRK"
BASE COUNT          274 a          172 c          111 g          307 t
ORIGIN      
        1 aaactaggaa gcgagccgca ggctgtactt gagtacggca aggcaaagct gacgtggttt
       61 gaagagattt tcaaagagta ttaaacaaaa agaaaaaccg ctactcctaa gaatagcgat
      121 ttaatcatgt ttttaagcta ctactgtagt tgctctatta tttaagtgtt atgaataact
      181 tcaatatctt taaagctttt tgttagattt ataatttcaa ctatagccat ctctactcct
      241 tagtttacag caaaaaatta tgcttaattc ttattgtttt tgtaaacaat cagcgaaccc
      301 aatactattc cattcaacaa gcctaaagaa ataccatatt caactggaaa atctaaaaaa
      361 attgcaaaca ctattccaat catcacccct agtgaggcta ttgttaaaat tacatattca
      421 ttttctttct tcattttctt tacacctcat ttcctttttg tatataagct gtagtactct
      481 ccaccaagag tcattaagta cttatgattt cctgactcaa caatcttacc acctttcatt
      541 acaaaaataa tatccgcatc cttaatcgtt gacaatctat gagctataat tatttgagta
      601 caaccctgac tttgtatata ctttgttatt ctttcctcat taatagtgtc taatgcacta
      661 gttgcttcat ctaaaattac aatactagga ttatttatta atgcacgtgc cagtgctatc
      721 cgttgccttt gcccacctga aatatttgac cccatctctg agatgatagt attaaatttc
      781 atcggcatag ccatgatttc atcatagatt tgaactgctt tacaaacttc ctctatcttt
      841 tgtgaagtaa cttcgtgctt taaa
//
LOCUS       DAVMEF010000310          861 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.311, whole genome shotgun sequence.
ACCESSION   DAVMEF010000310 DAVMEF010000000
VERSION     DAVMEF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.311"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..119)
                     /locus_tag="VYQ30_002081"
     CDS             complement(<1..119)
                     /locus_tag="VYQ30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1967117.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVA"
     gene            complement(106..>861)
                     /locus_tag="VYQ30_002082"
     CDS             complement(106..>861)
                     /locus_tag="VYQ30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1967118.1"
                     /translation="YNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNS
                     GGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYSVGMAL
                     EQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLMKELND
                     GKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGV
                     VAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
BASE COUNT          259 a          177 c          117 g          308 t
ORIGIN      
        1 gcaactttta tactagatac atttttataa accttaggaa aatctgtata ataagtccag
       61 tcagctactg tgtcggtcat cgttgacaac cactcatcaa aatttctagt ttgtttcatt
      121 ttctgcctcc aacctcttcc ttgcaatttt taaatattct ttttcagcat cgatacctat
      181 aaatcttcta cctaaccgct tcgcaacaac acccgtagtg ccactaccaa caaatgggtc
      241 tagaatatag tcaccctctt tagtagaggc taaaataata cgttctaaca aatactctgg
      301 tttttgagtt ggatgtttcc cagcccattt ttcaactttc tttgttaaag aaccggtcca
      361 gacatctttc atttgttttc catcattcaa ttctttcatt aaatcataat tgtagtaatg
      421 acgagctttt ttatcatttt ttctggccca taaaatggtt tcagtagaat gggtaaaata
      481 acgacaagat aaattggggg cagggtttgt tttctgccaa gtaatattat tcagaatttt
      541 aaaaccttct tgttctaatg ccattccaac tgagtatatg ttgtgcaaac tacctgaaat
      601 ccataccgtc ccattaggct tcagaacttc ttttgctagg cgaatccatt tacgattaaa
      661 ctcatgtttt tcttcgaatg aagaaatttt atcccaatct cctttatcaa cagaaactac
      721 ttgtccccca gaattagata ttccaccatt acttaaaaaa taaggtggat cagcaaaaat
      781 catatccata ctttctggtt tcatttttga taagaacttg aacgtatctg aatgaacaag
      841 aatcatctta tttttattgt a
//
LOCUS       DAVMEF010000311          848 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.312, whole genome shotgun sequence.
ACCESSION   DAVMEF010000311 DAVMEF010000000
VERSION     DAVMEF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.312"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..833)
                     /locus_tag="VYQ30_002083"
     CDS             complement(<1..833)
                     /locus_tag="VYQ30_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967119.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     I"
BASE COUNT          263 a          141 c          137 g          307 t
ORIGIN      
        1 tctatcttca tcaacattaa aggattgtta taaatcttac ataactctct tgcttctata
       61 taataatttt taacttgttc tctgtctaga aatttggctc cagcatttcc tacaagaata
      121 agtagaggag ccaattggta gcttgtctgt ctttgtttac agagttcaat cgtttcaaga
      181 gcttcttgga tggcttcatt atatttttcc tttgatacta ggtagtgagc gtagttgtaa
      241 cgaactctga tgtagccaaa taaaaactct tgatggtcca aattttttgt ctgatacaac
      301 tctattaaat gagagtagtt tgcctcatat tcttgttcac gacccactaa ggaatagaaa
      361 ttagatagag tattcaacgc ctttaaataa atcagagtat ttgaagagac ttttaataat
      421 atattttcca atgaagaaat tgcctcacac ttactgtcat attgatagaa gtcaataata
      481 gctttaatcc attcaaggta agttcggtct tctagtgtta gaaaagtact tcgttcaatc
      541 tctattctat aaatatattc caaatcgtca taatttctat cgtctaatag tcgagcagac
      601 aattgcttga aattagagag gttagattta atttcaattt gttcattaaa aaaataatct
      661 aatggtactt caagtcgttg agagagtttg aacaaaaggt ctgcggaggg aatgaaatgc
      721 cctctctcaa ttttactaat ctggctttgt tcacaaattc cttctgcaag agtttgttgg
      781 gagagtctca actcttttct tttcaatctg aatttctctg cgagtgtact cattaaagat
      841 aataaacc
//
LOCUS       DAVMEF010000312          831 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.313, whole genome shotgun sequence.
ACCESSION   DAVMEF010000312 DAVMEF010000000
VERSION     DAVMEF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.313"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            402..773
                     /locus_tag="VYQ30_002084"
                     /pseudo
     CDS             402..773
                     /locus_tag="VYQ30_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
BASE COUNT          261 a          101 c          185 g          284 t
ORIGIN      
        1 tagctataat ctttccatgg ggctggtcga tataatgtaa gggagcttga gttgttagtg
       61 gaggataaaa tgttaagagt aatagtagtt attcaatctt tagtaataat actgcttgct
      121 gttgctttat tattatctcg gaaaaacttt gattcgttaa atgttgttct tataatgatg
      181 atcgtatcac aattgttgtt attagtatat ttgataaaat cagggaatca gtgattagct
      241 gttatttgaa ttaagtaaac tgttaagctt caagcagtaa ctggttttaa atatttaaaa
      301 taagataaag gaaggaagtc taatttaggt aactaaacaa agcctgcgat cacttgttag
      361 gaaaccatcc taaccatgct taataaaaaa ggagaaaata aatggaactc gtattaccaa
      421 ataattatgt tgtgattgat gaagaagaga tgatgtacct tgatgggggg ctatatatat
      481 acccaggtgg gcaattacag gagccattac tggtgcagca tatgcagcat tagcagcagc
      541 aggaggtgga ggccttcaac taagttcttg catcttatgg attacgctcc gcactggtag
      601 ctgggattgt taaaggttta ggagtattag gaattcatat tggaaatgct tttgcaaata
      661 ctgttattag aagtattgca tctgctggaa ttggtgctgg agctgattgg atttttacca
      721 atattattga tggctgggat gggcgacgtg ataatcaatt gagaataggt taataatctt
      781 atgttgattg ttttaatttc agttattgtt gcattcctgt atcataaaat t
//
LOCUS       DAVMEF010000313          822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.314, whole genome shotgun sequence.
ACCESSION   DAVMEF010000313 DAVMEF010000000
VERSION     DAVMEF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.314"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..329
                     /locus_tag="VYQ30_002085"
     CDS             <1..329
                     /locus_tag="VYQ30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1967120.1"
                     /translation="IEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
BASE COUNT          307 a          108 c          155 g          252 t
ORIGIN      
        1 aaatagagga gaaaaaggag gaagaaaata aacctacttt tgatgtatcg aaaaagaaag
       61 ataacccaca agtaaaccat agtcaattaa atgaaagtca cagaaaagag gatttacaaa
      121 gagaagatca ttcacaaaaa tctgattcaa ctaaggatgt tacagctaca gttcttgata
      181 aaaacaatat cagtagtaaa tcaactacta acaatcctaa taagttgcca aaaactggaa
      241 cagcaagcgg agcccagaca ctattagctg ccggaataat gtttatagta ggaatttttc
      301 ttggattgaa gaaaaaaaat caagattaag ataaaagcta tagaaaaaaa tggtttatgt
      361 actgagatta gatagtgagg tgatgacata gttttgtgaa aacagccatt tataactcaa
      421 ttatttagtt tacttcacta ttgatactat ttggagttat taatggactt agtttagata
      481 actaatgaat tgattgaaag ggttagtatt gacaatattg gtcatattga ttagaaaata
      541 gagtctatca aaatttaaag gctaatagag gtgatgagac aatttcaagg aattaagtag
      601 cgtttggttg cttaattcct ttttatttgg aaggaagttt ttcgtaaccg cccaataacg
      661 aagtctattg gacggttacg atatgatcac tgacttcgtc agtcttatct acaacctcaa
      721 aactgtgttt tgagcaacct gcggctagct tcctagttta ctctttgatt ttcattgagt
      781 attagaacag aaaaaatgct tggagtattt gtttgtgtgt tt
//
LOCUS       DAVMEF010000314          820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.315, whole genome shotgun sequence.
ACCESSION   DAVMEF010000314 DAVMEF010000000
VERSION     DAVMEF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.315"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(624..>820)
                     /locus_tag="VYQ30_002086"
     CDS             complement(624..>820)
                     /locus_tag="VYQ30_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967121.1"
                     /translation="FDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSVLLLF
                     SNFSVPFIKEKEFELFKNSK"
BASE COUNT          304 a          154 c          100 g          262 t
ORIGIN      
        1 aactcccaaa atagataaca ctattttcac aaaactcagt tccgaaccta tgacatatat
       61 atgaaaaaga ggggctagtc ctacacatat atacaataag tagccaacaa gttttatctc
      121 accaattcct gcaaaaaaat gtttcatgta acgattctcc atttactcca ttaaatcagt
      181 tttattttag actatcataa aacaatcacc tcttcaataa aaaccattgg cgaaatacta
      241 ttctgaagca ttgatagctc agatttctca gaataaagct gtcctaacta tcgagcagct
      301 caaatacata tatatgagca attctaagcc tagaattgct catatatatg ttttcgggct
      361 attctaagtc tttgactgct taaaatttta cattctgaag gattctaaac ctagaatagc
      421 tcaaaatcac gagaactaag gtattcgaaa cttcagagta atacaatccc cataggaatt
      481 ctccattctt attaagcatg attaaaatgt aatcctaaca agtgatcctc gactgtgttt
      541 gtagcactta gttacctaga taagactatt tccctttatc atatctgatt ttaagccgtt
      601 ctaagattta acctatgttt ctattacttg ctatttttaa ataattcgaa ttccttttct
      661 ttgataaatg gaactgaaaa attagaaaaa agaagtaaaa ccgaaaacaa atagcctgct
      721 ttttgaatca ctaaaagata tcctaaaaat aagtaagcta taacatccaa tgcgaaaaga
      781 aaaaatattc tcctcttcat taaagtactt gtgtcaaata
//
LOCUS       DAVMEF010000315          814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.316, whole genome shotgun sequence.
ACCESSION   DAVMEF010000315 DAVMEF010000000
VERSION     DAVMEF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.316"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>814)
                     /locus_tag="VYQ30_002087"
     CDS             complement(<1..>814)
                     /locus_tag="VYQ30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV1967122.1"
                     /translation="FTKWTGGKRQLLPVIRELIPKTYNRYFEPFVGGGALFFDLAPKD
                     AVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRSADRDERIDMMSEV
                     QRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELISAISVYINNNQLEI
                     KVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFADQVRLRDAFKRLSD
                     TGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISEIIVTNYE"
BASE COUNT          275 a          156 c          102 g          281 t
ORIGIN      
        1 ttttcataat ttgtgactat aatttcagaa atttttcctc gacttgaaga ttttgctcca
       61 ttagttcggg tagcttcaac ataatgtata ttaaaatcct tatacaactc ctctactaaa
      121 gcactagaag aatttgataa cataacataa gctcctgtat cactcaatct cttaaaggca
      181 tctcttaatc ttacttgatc tgcaaaagag aatccctcat gagtataaga cgtaaatgca
      241 ctcgtctcag acaatggaat atatggaggg tcaaaataca caaaatctcc tgttcgaaca
      301 tctacaattg ccttttcaaa atctcccact ttaatttcta gttgattgtt atttatataa
      361 actgaaatag cagatatcaa ttcctcatca acaattttag gattcttata acgtccatat
      421 ggaacattaa attgattctt agaattcaca cgatatagac cattaaagtt cactctcaac
      481 atatatagaa tacgtgcagc tctttgtact tcggacatca tatctattct ttcatcacga
      541 tctgcagaac gtaaatctaa ataatattct tttgaattat attcctgatg aactttcaaa
      601 atttcaatca attcttgagg attgtcctta atttgttgat agcaatttat tagttcagcg
      661 ttaaaatcat taataactgc atctttagga gccaaatcaa aaaataaagc tccacctcca
      721 acaaaaggtt cgaaatacct gttataggtt ttaggtatta attctctaat aacaggcaat
      781 aattgtcttt taccacctgt ccatttcgtg aacg
//
LOCUS       DAVMEF010000316          803 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.317, whole genome shotgun sequence.
ACCESSION   DAVMEF010000316 DAVMEF010000000
VERSION     DAVMEF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.317"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(244..555)
                     /locus_tag="VYQ30_002088"
     CDS             complement(244..555)
                     /locus_tag="VYQ30_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967123.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(570..>803)
                     /locus_tag="VYQ30_002089"
     CDS             complement(570..>803)
                     /locus_tag="VYQ30_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1967124.1"
                     /translation="NEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIK
                     VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          253 a          144 c          136 g          270 t
ORIGIN      
        1 agacggattg ttttgacaat tctaatcttt tagactttgc ataataactg ttaaacagtt
       61 gaaaattgac ccagttttaa caagtagtca aaataccaga taaaggaaaa aagtctttaa
      121 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      181 ggaagattta cttcattttc tcttggaatt gagttttctc ctacttcaaa actagatgat
      241 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      301 aaaggggcaa cttaagcttt ctttatattg cttatatgtt agtagtttta gacaaacacc
      361 acataaaata ggcttatcct gtatcaaaga taagggatac ggctcaaaag ggtgattttc
      421 taatgaatca tgacaccgat aacaagcata atacttttta caatcatagc attgtaaagc
      481 aataatatcc agcttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      541 ctgtgcctct atcatttcct tttcctttct taagcacgaa ctgtgacgct ggttccatct
      601 tccttataaa gattgatgag tccttctttt aatgcacgaa ccatgtctcc tgattgaacg
      661 ggttgaggca ccttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      721 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      781 aactcaactt ggtccccttc gtt
//
LOCUS       DAVMEF010000317          785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.318, whole genome shotgun sequence.
ACCESSION   DAVMEF010000317 DAVMEF010000000
VERSION     DAVMEF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.318"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..428
                     /locus_tag="VYQ30_002090"
     CDS             <1..428
                     /locus_tag="VYQ30_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967125.1"
                     /translation="VIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGG
                     EQQKVAIARAVIADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIEL
                     ASKTDRALILKDGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            431..>785
                     /locus_tag="VYQ30_002091"
     CDS             431..>785
                     /locus_tag="VYQ30_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967126.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFI"
BASE COUNT          247 a          101 c          174 g          263 t
ORIGIN      
        1 cggtaataga aaatatagca cttcctttgc gattatcaca aaaaaaatta actattaaaa
       61 atgtagaaaa cttactcaaa agaatgaagt ttaatgctgg cttaaacgat tttgttggaa
      121 ctctgtctgg tggagaacaa cagaaggttg ctatagctag agcggttatt gctgatagtg
      181 atataatatt tgctgatgag ccaactgggg ctttagacag cgtttctcgt gaagtaattt
      241 ttgaattatt gagagagtta gtaggggcgg gtaagtgtgt aattatggta acgcacgata
      301 tagaattggc ctcgaaaact gatcgtgcat taatattgaa agatggaaaa attttcaaag
      361 aacttcatag acctagcggg gaagagttgt ataaaatctt agaggtacaa tcaactacgg
      421 aggaatagag atgctcagat tagtttacta tcaattttta cataataaga aacaatggtt
      481 aggcgtttca ccagtaattt ttgtttcgag tttagttatg ggattggctg ttaatggggt
      541 aattaatgtt gagaataatt cgcaagtatt tgtcggactt ccagatccta aaccaatttt
      601 tatgtttccg atagtattcg gaggagtaac attattcttt gtgctctcta acataataaa
      661 tatgttggtt gaaattttta gagatgatta cgaacttctg gaggtgttgg gagcaagtcg
      721 ccttcaacta tcatttcttg tggggggaca gatttttatc atttcctcga ttattagttt
      781 tattg
//
LOCUS       DAVMEF010000318          766 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.319, whole genome shotgun sequence.
ACCESSION   DAVMEF010000318 DAVMEF010000000
VERSION     DAVMEF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.319"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>766)
                     /locus_tag="VYQ30_002092"
     CDS             complement(<1..>766)
                     /locus_tag="VYQ30_002092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967127.1"
                     /translation="LAEADALVLAEADALVDAEADALVLADSDALVLAEADALVLAEA
                     EALVDAEAEALVEAEAEALVDAEADALVDAEADALVDAEAEALVLAEAEALVDADSDA
                     EVLAEAEALVLAETDALVDAEAEALVDAEADALVLAEAEALVLAEADALVDAEAEALV
                     DADSDALVDADSEADVLAEADALVDAEADALVLAEADALVDADSEADVLAEADALVDA
                     DSEALVDAEAEALVLAEADALVDAEADALVLAEADAL"
BASE COUNT          186 a          257 c          179 g          144 t
ORIGIN      
        1 cgagtgcgtc cgcttcagca agtactagtg catcagcttc agcatcaacg agtgcgtccg
       61 cttcagcaag taccagtgcc tcggcttcag cgtcgacaag tgcctctgaa tcggcatcaa
      121 cgagtgcgtc agcctcagca agcacatcag cttctgaatc tgcatcaacc agtgcgtcag
      181 cctcagcaag taccagtgcg tcagcctcag cgtcaacaag tgcgtcagcc tcagcaagca
      241 catcagcttc tgaatctgca tcaaccagtg cgtctgaatc tgcatcaacg agtgcctcag
      301 cctcagcgtc aacgagtgcg tcggcctcag caagcaccag cgcctcggct tcagcaagta
      361 ctagcgcgtc agcctcagcg tcaaccagtg cctcagcctc agcatcgaca agtgcatcag
      421 tctcagcaag caccagtgct tcggcttcag caagcacctc agcgtctgaa tcggcatcaa
      481 ccagtgcctc agcttcagca agcacaagcg cctcagcttc agcatcaacc agtgcatcag
      541 cttcagcgtc aaccagtgca tcggcttcag cgtcaaccag tgcctcagct tcggcctcaa
      601 caagtgcctc agcttcagcg tcaacaagcg cctcggcttc agcaagtact agcgcgtcag
      661 cctcagcaag caccagtgca tcagagtcag caagtaccag tgcgtcagct tccgcatcaa
      721 cgagtgcgtc agcttcagca agcacaagtg cgtcagcctc agcaag
//
LOCUS       DAVMEF010000319          758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.320, whole genome shotgun sequence.
ACCESSION   DAVMEF010000319 DAVMEF010000000
VERSION     DAVMEF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.320"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..672)
                     /locus_tag="VYQ30_002093"
     CDS             complement(<1..672)
                     /locus_tag="VYQ30_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1967128.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVE"
BASE COUNT          259 a          147 c           90 g          262 t
ORIGIN      
        1 ttcaactgat ttccagatta tcaaaatctg agatttatag cacaatattg atgatattct
       61 atcaatataa tgtttttcat caagttcctc ttgatacatt tttaattctt tagtttttcc
      121 catataacta accatactac tatcacttac atatgggaag tcctcataat atattacttt
      181 ataacgcata aattcaagcg cccttccaat actattcaca aaaacatgat caacatggtc
      241 accaagtgaa agcggacaat atacgacaca tttgtcgtct aaatgcatta acagctcttt
      301 tatgatatca ttctttaatg tgtcctcatt ttttaattca ctatagatat gacggtatag
      361 aaaattgcca tttctatctt tcctatagag acattcatag tacgataagt gtctaaaatc
      421 acactgtaga cgttcacaag ctaacctgtc ttctttcttc ctttcttcaa tcggatattt
      481 cccaaggtta cacaacttat gaaattgctt agcagagggc tgtagctgtt ggctcaaagg
      541 gtaaccagaa aatatagtaa taacaagtac aatttctcct tctgaagtta attttgaaat
      601 ataatcacca caggaaaaaa ttgcgtcatc taaatgtgga gataaaaaga tatacttagt
      661 attgttactc ataaccattc cctctacaat ttatctaaaa actcactaag tgtctgatta
      721 aattccacat catcaaaaaa attcacctta ttcttaat
//
LOCUS       DAVMEF010000320          735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.321, whole genome shotgun sequence.
ACCESSION   DAVMEF010000320 DAVMEF010000000
VERSION     DAVMEF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.321"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          245 a          134 c           91 g          265 t
ORIGIN      
        1 ttttaatatc atttacctac ttccaagatg ctgaaaaata ctaagtcaca ctattatgat
       61 gttattattt ccatttttat tttaacagat gagtcaattc tttcaactac cccctccctt
      121 ataactatat tcatgaagta ttcttgataa taaacctcat ttaacttctc aaagtagtca
      181 gcttctgcaa ctgctattat ctcataacag attgatttgg tttcaataaa tggaagctag
      241 aagccttgtc agctaccctg cacagttggt gttttttcat ttttctcttc aaaaaaattg
      301 gtcggaagtt ataataaaaa ggtgatgtgt ttgtggtgag ttgaattaag agacaaactc
      361 actttgtctt aacttttaaa ttgcctaaca aaaatacgga gattattctc taagctttaa
      421 ctgcaacatt atgtctctac aaccaacgat aagtccagaa atctacccaa ataaccaaac
      481 aacctacatt tgttgaactt tgatgacaag taggaaattt tacatttgat aaacactatt
      541 ttgtacgcat tttgcacata atttaacgtg ctttccatca atttagttaa ggacttacat
      601 agaaaacaat ccgtttttgc ttatgttaaa ttagtagaat tagacaaaaa caaaaccgcc
      661 gttcgcactg ttatatattc atttttagta cttactattc taaaaaagat atctatcttt
      721 caattcctca atttc
//
LOCUS       DAVMEF010000321          718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.322, whole genome shotgun sequence.
ACCESSION   DAVMEF010000321 DAVMEF010000000
VERSION     DAVMEF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.322"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..718
                     /locus_tag="VYQ30_002094"
                     /pseudo
     CDS             27..718
                     /locus_tag="VYQ30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          232 a          131 c          153 g          202 t
ORIGIN      
        1 acgccttcaa atcattctat agtaaaatga aacaagaaca ggacaaatcg atcaggacag
       61 tcaaatcgat ttctaacaat gttttagaag tgggggtgta ctattctagt ttcaatctac
      121 tatatttcgt ctgatgggca aatcttataa agagattata gagcttttag actgtaatca
      181 aacaacgatt tagcgaaatg taaaaaaata tgaggagttc ggactcgact ctctccttca
      241 agaaacacgt agtggtcgta accatgcata tatgacagtt gaggaagaga aagcctttct
      301 tgcccgtcat ttgaaggctg cagagtcagg agaatttgtt acaattgatg ccttatttca
      361 ggcttataaa aaggagttag gtcgttccta cacacgtgat gccttctatc aactgttgaa
      421 gcgccatggc tggcgaaata ttatgccacg tccagaacat cctaagaaag cagacgctca
      481 aaccattgtc gcgtctaaaa ataaaatctc aattcaagaa gacaagaaag cgctttaaaa
      541 tcagtagacg ttttcgtaag gttcgcttga tgtaccaaga tgaggctggt ttcagtagaa
      601 tcagtaaact gggatcttgt tgggctccaa taggagtagg tccacatgtc catagtcact
      661 atatacgaga atttcgctat tgttatggag ctgttgatgc ccatattgga agatgtca
//
LOCUS       DAVMEF010000322          698 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.323, whole genome shotgun sequence.
ACCESSION   DAVMEF010000322 DAVMEF010000000
VERSION     DAVMEF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.323"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..505
                     /locus_tag="VYQ30_002095"
     CDS             <1..505
                     /locus_tag="VYQ30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1967129.1"
                     /translation="LTRYPNPQKRLEARFDKLIEIKRLTASKIQDILSIAPRSIGTTS
                     PAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNIH
                     AFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFKA
                     YLKTKLE"
BASE COUNT          210 a          153 c          137 g          198 t
ORIGIN      
        1 cttgacacgc taccctaatc ctcagaaaag gcttgaggca agatttgata agctgataga
       61 aattaagcga ttgaccgctt ctaaaattca ggatatcctc tcaatcgcac ctcgttctat
      121 cggaacaaca tctcctgctc gtgaattcga aatcatcgaa atcatcaaac attacaagag
      181 gctcattgac aaggcggaaa catgtgtcaa tgacttgatg gctgagttca actcggtcat
      241 cacgacggtt actgggattg ggggtcgttt aggggcggtc attttagccg agattcgaaa
      301 tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg attcttctat
      361 ttatcagtca ggtcagattg atttagctgg aagaatgatc aaacggggtt cccctcatct
      421 gcggtgggca ctcatacaag ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc
      481 ctatcttaag actaagttag aataaggaaa acattacaat gtagccatca tccaccttgc
      541 aaaaaaactt atccgaaccc tgttttatat cctaaaaaag agctgccatt tgacgaacaa
      601 aaagtgatgt aatttctgaa attaacacag gattaccttt gaaaaaatgg actttttcaa
      661 gggctttttg ccgtgctcta aaatcatcgc caaatcat
//
LOCUS       DAVMEF010000323          683 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.324, whole genome shotgun sequence.
ACCESSION   DAVMEF010000323 DAVMEF010000000
VERSION     DAVMEF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.324"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            48..395
                     /gene="ssrA"
                     /locus_tag="VYQ30_002096"
     tmRNA           48..395
                     /gene="ssrA"
                     /locus_tag="VYQ30_002096"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(568..655)
                     /locus_tag="VYQ30_002097"
     tRNA            complement(568..655)
                     /locus_tag="VYQ30_002097"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(619..621),aa:Ser,seq:gga)
BASE COUNT          193 a          133 c          137 g          220 t
ORIGIN      
        1 ttttgcattt ttgagttaga taaggtataa tggttttatt gtcttttggg gtcgttacgg
       61 attcgacagg cattatgagg catattttgc gactcgtgtg gcgacgtaaa cgctcagtta
      121 aatataactg caaaaaataa cacttcttac gctctagctg cctaaaaacc agcaggcgtg
      181 acccgatttg gattgctcgt gttcaatgac aggtcttatt attagcgaga tacgattaag
      241 ccttgtctag cggcttgata agagattgat agactcgcag tttctagact tgagttatgt
      301 gtcgaggggc tgttaaaata atacataacc tatggttgta gacaaatatg ttggcaggtg
      361 tttggacgtg ggttcgactc ccaccggctc cattattcct ttgcattcct tggtaaaacg
      421 ttgttaaatc aacgtttttt atttttatct ttggtattcc tttgcattct tttgctaaaa
      481 agggagtcac aaacagaccc tattttaaaa aaaggataga aaaaaggata caacatttgt
      541 cgcatcctaa aaataatctt ttttcgacgg aagacatggg attcgaaccc acgcacgcta
      601 ttacacgcct accgcgtttc caacacggcc tcttaagcct cttgagtaat cttccaatac
      661 ttactcaaat agtctaccat aaa
//
LOCUS       DAVMEF010000324          679 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.325, whole genome shotgun sequence.
ACCESSION   DAVMEF010000324 DAVMEF010000000
VERSION     DAVMEF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.325"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..607
                     /locus_tag="VYQ30_002098"
     CDS             <1..607
                     /locus_tag="VYQ30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1967130.1"
                     /translation="LDVVGVQDFKKEFCIPNNKKIICYVGRLDPEKRPDRFLEFAEKL
                     FLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSD
                     TEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDS
                     EYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG"
     gene            600..>679
                     /locus_tag="VYQ30_002099"
     CDS             600..>679
                     /locus_tag="VYQ30_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1967131.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAV"
BASE COUNT          237 a           64 c          148 g          230 t
ORIGIN      
        1 tttagatgta gtaggtgttc aagactttaa aaaagaattt tgtattccaa ataacaaaaa
       61 aatcatttgt tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt
      121 tgcagaaaaa ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag
      181 tatgtgggct gctttgaaag aaaaaatttg ccatttgaaa tgtagagata atttcaggtt
      241 acttggtgaa atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc
      301 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt
      361 tttagctagt aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt
      421 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
      481 tgagtatttg aaatatatag gtgagaatag taaatcaaaa ataagaaaac atttttctgt
      541 gcaaaaaatg tttgtagaaa ccatgagagt atatgatgaa ttattagaga agagtagtca
      601 tggataggaa tatagatcaa gaattagtaa gtattataat accaacacat aataggtatg
      661 aatctctgat tagagctgt
//
LOCUS       DAVMEF010000325          677 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.326, whole genome shotgun sequence.
ACCESSION   DAVMEF010000325 DAVMEF010000000
VERSION     DAVMEF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.326"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>677
                     /gene="phtB"
                     /locus_tag="VYQ30_002100"
     CDS             <1..>677
                     /gene="phtB"
                     /locus_tag="VYQ30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1967132.1"
                     /translation="NHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLIFDP
                     AQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSP
                     QSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSA
                     ETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVD
                     FEALDNL"
BASE COUNT          219 a          156 c          129 g          173 t
ORIGIN      
        1 agaaccacaa tctgactgtc actccaactt atcatcaaaa tcaaggggaa aacatttcaa
       61 gccttttacg tgaattgtat gctaaaccct tatcagaacg ccatgtggaa tctgatggcc
      121 ttattttcga cccagcgcaa atcacaagtc gaaccgccag aggtgtagct gtccctcatg
      181 gtaaccatta ccactttatc ccttatgaac aaatgtctga attggaaaaa cgaattgctc
      241 gtattattcc ccttcgttat cgttcaaacc attgggtacc agattcaaga ccagaacaac
      301 caagtccaca atcgactccg gaacctagtc caagtctgca acctgcacca aatcctcaac
      361 cagctccaag caatccaatt gatgagaaat tggtcaaaga agctgttcga aaagtaggcg
      421 atggttatgt ctttgaggag aatggagttt ctcgttatat cccagccaag gatctttcag
      481 cagaaacagc agcaggcatt gatagcaaac tggccaagca ggaaagttta tctcataagc
      541 taggagctaa gaaaactgac ctcccatcta gtgatcgaga attttacaat aaggcttatg
      601 acttactagc aagaattcac caagatttac ttgataataa aggtcgacaa gttgattttg
      661 aggctttgga taacctg
//
LOCUS       DAVMEF010000326          676 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.327, whole genome shotgun sequence.
ACCESSION   DAVMEF010000326 DAVMEF010000000
VERSION     DAVMEF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.327"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..473
                     /gene="rlmH"
                     /locus_tag="VYQ30_002101"
     CDS             24..473
                     /gene="rlmH"
                     /locus_tag="VYQ30_002101"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1967133.1"
                     /translation="MKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQK
                     ILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFSTLTFIIGGSLG
                     LSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            517..590
                     /locus_tag="VYQ30_002102"
     tRNA            517..590
                     /locus_tag="VYQ30_002102"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:551..553,aa:Arg,seq:tct)
BASE COUNT          227 a           98 c          136 g          215 t
ORIGIN      
        1 ttaaagttgt aacagttggg aaactgaaag aaaagtattt aaaagatggt atcgcagagt
       61 attcaaaacg aatttctaga tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa
      121 caccagataa ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa
      181 ttttatcaaa aattgctgac cgtgatttcg ttattgtgtt agccattgaa gggaaaactt
      241 tcttctcaga agaatttagt aagcagttag aagaaacttc tataaaagga ttttctactc
      301 ttacttttat tattggggga agtttaggat tgtcatcatc tgtaaaaaat agagccaatc
      361 tttctgtcag ttttggtcgc ctaaccttgc ctcatcagtt aatgagacta gttcttgttg
      421 aacaaatcta tcgcgctttt acgattcagc agggattccc ctaccataaa tagaaaattg
      481 acttttaatt gaatttttgg tagaataatt gtgttaggtc tcatagctca gctggataga
      541 gcattcgcct tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca atataatagc
      601 aaagctggga attttcccgg cttttttctt aaaaaagtac actttgggag aaaaaaatga
      661 cagttgagag aatttt
//
LOCUS       DAVMEF010000327          665 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.328, whole genome shotgun sequence.
ACCESSION   DAVMEF010000327 DAVMEF010000000
VERSION     DAVMEF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.328"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..656)
                     /locus_tag="VYQ30_002103"
     CDS             complement(<1..656)
                     /locus_tag="VYQ30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HEV1967134.1"
                     /translation="MVDAEADALVDADSDALVLAEAEALVDADSDAEILAEAEALVDA
                     EAEALVLAEADALVLAEADALVDAEAEALVDAEADALVLAEADALVDADSEADVLAEA
                     DALVDADSEALVDAEAEALVLAEADALVLAEADALVDAEADALVLADSDALVDAEAEA
                     LVDADSDALVLAEADALVDADSDAEILAEAEALVLAEAEALVLAEADALVDAEAEALV
                     "
BASE COUNT          163 a          214 c          157 g          131 t
ORIGIN      
        1 tcaaccagtg cttcagcttc cgcatcaacg agtgcgtcgg cttcagcaag caccagtgct
       61 tcagcctcag caagtactag cgcctcagcc tcagcaagta tctcagcgtc tgaatcggca
      121 tcaacgagtg catcagcctc agcaagtact agtgcatcag agtcagcatc aacgagtgct
      181 tcggcttcag cgtcaaccag tgcatcagag tcagcaagta ccagtgcgtc agcttccgca
      241 tcaacgagtg cgtccgcttc agcaagcacc agtgcgtcag cctcagcaag taccagtgcc
      301 tcggcttcag cgtcgacaag tgcctctgaa tcggcatcaa cgagtgcgtc agcctcagca
      361 agcacatcag cttctgaatc tgcatcaacc agtgcgtcag cctcagcaag taccagtgcg
      421 tcagcctcag cgtcaaccag tgcttcagct tccgcatcaa cgagtgcgtc ggcttcagca
      481 agcaccagtg cgtcagcctc agcaagtact agcgcctcag cctcagcatc aaccagtgcc
      541 tcagcctcag caagtatctc agcgtctgaa tcggcatcaa cgagtgcttc agcctcagca
      601 agtactagtg catcagagtc agcatcaacg agtgcgtcgg cttcagcgtc aaccagtgca
      661 tcaga
//
LOCUS       DAVMEF010000328          657 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.330, whole genome shotgun sequence.
ACCESSION   DAVMEF010000328 DAVMEF010000000
VERSION     DAVMEF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.330"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(100..309)
                     /locus_tag="VYQ30_002104"
     CDS             complement(100..309)
                     /locus_tag="VYQ30_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967135.1"
                     /translation="MFFAGIGEIHFLLFLLYLSMSASPIIGLVIGYTKINLINSIIIA
                     LGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          252 a          130 c           72 g          203 t
ORIGIN      
        1 tcaatatttg ataaagcaaa gctttaaacc cttccccaga cgttagcttt tattcaaccc
       61 actacagttg acaaggagcc caaattattg ataaaaactt tagtcatcat cgtctctata
      121 aaaatatttt ctatacactc ttgataatac caaaattaat gccaatatca taccaagagc
      181 tataataata gaatttatta gatttatttt agtatatcca atcactagtc caataattgg
      241 actagcagac atgcttagat ataataaaaa taacaaaaaa tgaatttcac caattcctgc
      301 aaaaaaaatg tttcatagct atagttttac gcccataaat acccatcaaa tttaccgtct
      361 acgatatcta aaccatatgc aatggctcct cctaatgaga agacttcagc aacagtagcc
      421 gcaatattaa gagcattagt aatagttgcc atactaactc caccaatata tcttgttata
      481 gcacttagtt acctagataa gatatccttt agcttgcttc caaaatttaa gtctgcacct
      541 ataatatgag ttcatattag tactgctaaa tcctatctat agtaatgtca aacacctaaa
      601 taaagtcatc tctccaattc attcaagaaa tgaagaaagg caaaaaatct ttaaaat
//
LOCUS       DAVMEF010000329          650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.331, whole genome shotgun sequence.
ACCESSION   DAVMEF010000329 DAVMEF010000000
VERSION     DAVMEF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.331"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>650
                     /gene="phtB"
                     /locus_tag="VYQ30_002105"
     CDS             <1..>650
                     /gene="phtB"
                     /locus_tag="VYQ30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1967136.1"
                     /translation="DKKESNRVAYIDGDQAGQKAENLTPDEVSKREGINAEQIVIKIT
                     DQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDSDIVNEIKGGYVIKVNGK
                     YYVYLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGRYTTDDGYIFNA
                     SDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAQAYWNGKQGSRPSSSSSYNAN"
BASE COUNT          212 a          118 c          152 g          168 t
ORIGIN      
        1 aggataagaa agagtctaat cgagttgctt atatagatgg tgatcaggct ggtcaaaagg
       61 cagaaaactt gacaccagat gaagtcagta agagggaggg gatcaacgcc gaacaaattg
      121 ttatcaagat tacggatcaa ggttatgtga cctctcatgg agaccattat cattactata
      181 atggcaaggt tccttatgat gccatcatca gtgaagagct cctcatgaaa gatccgaatt
      241 atcagttgaa ggattcagac attgtcaatg aaatcaaggg tggttatgtc attaaggtaa
      301 acggtaaata ctatgtttac cttaaggatg cagctcatgc ggataatatt cggacaaaag
      361 aagagattaa acgtcagaag caggaacgca gtcataatca taactcaaga gcagataatg
      421 ctgttgctgc agccagagcc caaggacgtt atacaacgga tgatgggtat atcttcaatg
      481 catctgatat cattgaggac acgggtgatg cttatatcgt tcctcacggc gaccattacc
      541 attacattcc taagaatgag ttatcagcta gcgagttagc tgctgcccaa gcctattgga
      601 atgggaagca gggatctcgt ccttcttcaa gttctagtta taatgcaaat
//
LOCUS       DAVMEF010000330          649 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.332, whole genome shotgun sequence.
ACCESSION   DAVMEF010000330 DAVMEF010000000
VERSION     DAVMEF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.332"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(133..>649)
                     /locus_tag="VYQ30_002106"
     CDS             complement(133..>649)
                     /locus_tag="VYQ30_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1967137.1"
                     /translation="KQKWHIYAGQYLGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIP
                     IYLGIRFAIVGEDAEEEEEEIIERLEQSKANQLFWTVTLLTIASGGDNLGIYIPYFAS
                     LDWSQTLVALLVFVIGIIIFCEISRVLSSIPLIFETIEKYERIIVPLVFILLGLYIMY
                     ENGTIETFLIV"
BASE COUNT          226 a          149 c           99 g          175 t
ORIGIN      
        1 tcctaatcaa atcaaaagcc actctacaat agagtaactt taagattgtc catttatact
       61 caatgaaaaa gccctctgaa aatcagagag ctgatttgag ctcgggctaa aatcctagtg
      121 aaacaaaaaa atctacacga tcagaaaagt ctctatcgtg ccattttcat acatgatgta
      181 tagtccaagt agaatgaata ctaagggcac aatgattcgc tcgtattttt caattgtctc
      241 gaatattaac ggaatagagg ataacacccg actaatctcg caaaagataa ttatgccgat
      301 tacaaacaca agcaaggcca cgagggtctg tgaccaatct aacgaagcaa aataaggtat
      361 atagatacct aaattatctc cgccagacgc aattgtcagc aatgtaactg tccaaaacag
      421 ttgatttgcc ttgctttgtt ctaatctttc aataatttct tcctcttctt cttccgcatc
      481 ttctccaaca attgcaaagc gaatccctaa atagataggg attaaaccaa gcaatccaac
      541 catccattct tcaggcacga aattaacgac ataagcagca actaaactcg cccctacaag
      601 taagcctgtg cctagatatt gccccgcata aatatgccat ttctgttta
//
LOCUS       DAVMEF010000331          628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.334, whole genome shotgun sequence.
ACCESSION   DAVMEF010000331 DAVMEF010000000
VERSION     DAVMEF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.334"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>628
                     /locus_tag="VYQ30_002107"
     CDS             <1..>628
                     /locus_tag="VYQ30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967138.1"
                     /translation="IFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKD
                     AKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGKDIKEISTE
                     SLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDT
                     VIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQ"
BASE COUNT          250 a           81 c          129 g          168 t
ORIGIN      
        1 aaatatttta tttatcgccc aaaatagaaa gattaaaaga aattcaaaat caagatttac
       61 aagaaggcga tgactatagc ttaaaaaaat ttgatattga tctaaaagat gttgagtttg
      121 cctacaataa agacgcaaaa gttttaaatg gtgtaagttt taaagctaag cagggagagg
      181 tcactgcttt ggtaggtgca agtggctgcg gtaaaacaac tatcttgaaa cttatatcaa
      241 gactttatga ttatgacaag ggacaaatct taatcgatgg caaagatata aaagaaatat
      301 caacagaatc tctttttgat aaggtctcta tagttttcca agatgtggtt ctctttaatc
      361 aaagcattat ggaaaatatt agaatcggaa agcaagatgc aagtgacgaa gaggttaaaa
      421 gagcagcaaa acttgcaaat tgcacagatt ttatagaaaa aatggataaa ggtttcgata
      481 cagttattgg tgagaacgga gctgagctat caggaggaga aagacaaaga ttatcaatag
      541 ccagagcctt cttaaaagat gcgccgatat tgatcttaga tgagataaca gcaagccttg
      601 atgttaacaa cgagaaaaag attcaaga
//
LOCUS       DAVMEF010000332          621 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.336, whole genome shotgun sequence.
ACCESSION   DAVMEF010000332 DAVMEF010000000
VERSION     DAVMEF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.336"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..613)
                     /locus_tag="VYQ30_002108"
     CDS             complement(<1..613)
                     /locus_tag="VYQ30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEV1967139.1"
                     /translation="MFHLSEWKRNEEFDFFNKMDVPYLSMSSRIEVTQAINFHKKHSI
                     SLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTSEDKLNFSSFTMGDN
                     LIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVSTPMNLSKIDTVPRIT
                     WGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKIN"
BASE COUNT          209 a          112 c           83 g          217 t
ORIGIN      
        1 catttatttt atttacaaac ctttgcaata acacaaaaaa attagatacg tgtaatccat
       61 ctcccattcc atgattaatt tgtaaattca ctgtacaaaa atatctttgg ttctcttgaa
      121 taacttttcc ccacgttatt cgaggaactg tatcaatttt tgaaagattc ataggagtgc
      181 ttacatgtaa aaaatcaatc catggaacac aagaaagata agcaatgtta ttcttgtcaa
      241 tatttggttt ttgcccttct tctgccttct gtttatttat gttgaacgca gaaacaaatt
      301 caattaaatt atctcccatc gtaaatgaag aaaaattaag cttatcttcc gatgtaaggg
      361 tagtgaaaga acaacctctt tgattgtatt gccagacaat ctttccgtcg gtatccatta
      421 gtaattctgg aatagaattt attgcactca taacacacca cgatattatg gcataaagtg
      481 atatcgaatg ttttttatga aaattgatag cctgcgttac ttctattcgt gaactcatag
      541 ataaataagg aacatccatt ttattgaaaa agtcaaattc ttcattcctt ttccactctg
      601 ataaatgaaa aattccatcc c
//
LOCUS       DAVMEF010000333          619 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.337, whole genome shotgun sequence.
ACCESSION   DAVMEF010000333 DAVMEF010000000
VERSION     DAVMEF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.337"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>619)
                     /locus_tag="VYQ30_002109"
     CDS             complement(<1..>619)
                     /locus_tag="VYQ30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1967140.1"
                     /translation="KRSTNEFIGRSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVS
                     QTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFR
                     HQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVE
                     GLRPDSNEDGSIFFKLKEGIFKKFCQNAIDSKKIGGQDNFDEAWDL"
BASE COUNT          205 a          118 c           89 g          207 t
ORIGIN      
        1 aaagatccca agcttcatca aaattatctt gacctccaat cttcttagaa tctatagcat
       61 tctgacaaaa cttcttaaaa ataccttctt ttaatttaaa aaatatactg ccatcctcat
      121 ttgaatccgg tcttaaaccc tcaacaaaat ccgtataatc ataagatgga tgaaactgta
      181 caaatccgat ttggtcttcg ttgccatccg ttaattcttt agcaatttct ttagcaagat
      241 aagtttttcc cgtgccagga gcaccacgaa ggataaggtt tggagacttt aatagagatt
      301 gagttaattg atgacgaaaa ttgtttgatg aatccattgt actattgtct tccttatttt
      361 ccataaaaag cttagaatac tcaaaaagca tagttcctaa tttcgatggc tccaactcag
      421 gataagtttt ggacaccagt tcaattattt tatgattttg cagataaggt ccaccttgat
      481 ttgtctgaga aacaaagtgg ttccaaaatt ctttctgact attaatattt acaccaaaga
      541 aaggatctcc ctcagaatag atacaaagta attttgtcac catagcagaa cgaccaataa
      601 attcatttgt tgatctttt
//
LOCUS       DAVMEF010000334          618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.338, whole genome shotgun sequence.
ACCESSION   DAVMEF010000334 DAVMEF010000000
VERSION     DAVMEF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.338"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>618
                     /locus_tag="VYQ30_002110"
     CDS             <1..>618
                     /locus_tag="VYQ30_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV1967141.1"
                     /translation="QLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGD
                     QCDNCSAILDPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRW
                     RKNAINLTKRYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDW
                     AQKLQNNITDFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEP"
BASE COUNT          216 a           90 c          118 g          194 t
ORIGIN      
        1 tgcaattata cgagaagaaa tttttgtata caaaaaaaat taaacaactc tattgtacat
       61 ttgataatca attcttacct gataggttcg ttgaaggaaa atgcccgaat tgtggaaccc
      121 attcaagagg agatcaatgt gataattgct cagcaatttt agatcctata gatttagttg
      181 ataaaagatg ttctatttgt tccaatgaac cagaagttcg tgagacagaa cacttttatt
      241 atgtttttag tgagtttcaa aatttgttag agacttactt aaatgatgct gaagaaacag
      301 tgagatggcg aaaaaatgca ataaatctaa ctaaaaggta tttacgggaa ggtttacctg
      361 atcgagctgt tactcgtgat ctaccaaatg ggattccagt tcccattgat ggttttagag
      421 ataaaaaaat atatgtctgg tttgaagcgg tagcaggtta ctacacagca agtgttgatt
      481 gggcgcaaaa acttcaaaat aatattactg atttttggaa caatcggaca aagtcatatt
      541 atgttcatgg aaaagataac atcccatttc atactattat ttggccggca atactctcag
      601 gtttagaaat agagcctc
//
LOCUS       DAVMEF010000335          615 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.339, whole genome shotgun sequence.
ACCESSION   DAVMEF010000335 DAVMEF010000000
VERSION     DAVMEF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.339"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            45..416
                     /locus_tag="VYQ30_002111"
     CDS             45..416
                     /locus_tag="VYQ30_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967142.1"
                     /translation="MIKKNHILCEIVPGAVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
BASE COUNT          190 a           77 c          118 g          230 t
ORIGIN      
        1 atttttcttt aattaaaaag aaagaaaaga aatgaaagat tttcttgatt aaaaagaatc
       61 atattctttg tgagattgtt cctggggcag ttgcctttgc ttgtttctac ttatctattt
      121 ctatttcgtc agttttccaa tttcttttag tggttattaa cttagcttct attttggtca
      181 ctgtttacct cactagggaa atgcggataa aagagtttga agacgatgat ttggttagtc
      241 ctagaaccaa tcaactcatg tttatcggct tgacaggctt tatgtctatt atttgtttgt
      301 atagaggtat cacagcagga gaatcttatc aacaactaat cgcttatatt ggtgctattc
      361 tttgcttgat catcatgctt ctactcattt ggggtttgaa gtattataaa aagtaggggc
      421 taatgaagtt tttgcaattt aggaggtttc gatgacaatt caagatttgg atgaaaaatt
      481 ttaaaaaaca atgatatact aaacttgtca aagttgcaac gggtgctgct acattatgtt
      541 aaaaatcgct cttgattgat aagttggatt tgttaggaaa ggagtaaata attatggata
      601 agaaaaagat agttt
//
LOCUS       DAVMEF010000336          614 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.340, whole genome shotgun sequence.
ACCESSION   DAVMEF010000336 DAVMEF010000000
VERSION     DAVMEF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.340"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..285)
                     /locus_tag="VYQ30_002112"
     CDS             complement(<1..285)
                     /locus_tag="VYQ30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967143.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQKLMNKAVKDEVAQ"
     gene            complement(273..525)
                     /locus_tag="VYQ30_002113"
                     /pseudo
     CDS             complement(273..525)
                     /locus_tag="VYQ30_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
BASE COUNT          174 a          126 c           75 g          239 t
ORIGIN      
        1 ttgagccact tcgtctttaa cggctttatt cataagcttt tgtaattttt ctttactatc
       61 aattacttct gattttccgt tgtaatttat tgtaataggt tttaacttac ctaatttctc
      121 gacacgctca ttaatttgat cttttttgaa ggctgcatat gtttttccta agattttttc
      181 gaaaatatat ttatcagata gcggtttgcc ttcttcttca gcttggtttt tgtattgatt
      241 tgaaacataa ggaacaaatc cttcatagta acctaatgct cccataagtt caaaagcttg
      301 ttttctaatt caaaccattg caactcagat ttcagctttt cagataaatc ctgctcatcc
      361 aaataatgac ttgaaattag tgctgaactc gtttctgtat cctgtacagg ctgagcaccc
      421 ataccagcaa aaaaataaac tcgttcctag caagaccgaa caagctccta ttgcatattt
      481 cctcaaagaa aaacgctgct ttctctcaaa ttgaaattct ttcatcccat ctcccatcat
      541 tcattattac tgtatatttt gtatatcaga aatagtttgt attcacaaat ctttctagtt
      601 attcccttat catt
//
LOCUS       DAVMEF010000337          609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.341, whole genome shotgun sequence.
ACCESSION   DAVMEF010000337 DAVMEF010000000
VERSION     DAVMEF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.341"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..609
                     /locus_tag="VYQ30_002114"
                     /pseudo
     CDS             1..609
                     /locus_tag="VYQ30_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a          116 c          128 g          152 t
ORIGIN      
        1 attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta aattaaacct
       61 agaagacctt cttatggcca ctcttcaata tgtgcgagaa tatcgaactt atgaacaaat
      121 tgcggctgat tttggtattc acgaaagcaa cttaatccgt cggagccaat gggttgaagt
      181 aactcttgtt caaagtggtg ttacgatttc aagaactcct ctcagttctg aggacacggt
      241 aatgattgat gcgacggaag tacaaatcaa tcgccctaaa aaagaattag cgaattattc
      301 tggtaaaaag aaatgccacg ctatgaaggc tcaagcgatt gtcacaagtc gagggagatt
      361 gtttctttgg atatcactgt gaactattgt catgatatga agttgttcaa aatgagtcgc
      421 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
      481 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagctgaa
      541 gataaagcct ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
      601 aaagtagaa
//
LOCUS       DAVMEF010000338          609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.342, whole genome shotgun sequence.
ACCESSION   DAVMEF010000338 DAVMEF010000000
VERSION     DAVMEF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.342"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            276..>609
                     /locus_tag="VYQ30_002115"
     CDS             276..>609
                     /locus_tag="VYQ30_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967144.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYHPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGK"
BASE COUNT          232 a           99 c           87 g          191 t
ORIGIN      
        1 tattcgacaa gttatcctaa aagtgttatt atgttgttgt gttatagatt gattgaatct
       61 acctaaagga tcctattcaa ttactagaac tatcacatac tcaaggtcag ctcacaaatg
      121 agcaactatt ttggttacaa tatctactaa atttaagtca aacaaataat ttagtcaaaa
      181 ttaaaaaaat agaggaacat aaatatgatt acaaaacaga atgtaacagt gttctacgat
      241 ttttactaga taaaactgta aattctgaag gaaggatcac ttcttcaaca gaatttggaa
      301 atttcgtaag taatttatca ttccaacacg gaatagctgg actactgttt cctctaaata
      361 aattgtacca tccagaactg gattctaaaa tactctctat catcaagaag gcagtgacaa
      421 ttagaacgac acacacatat gaatatcaat actcactgct atttggtgat gcaggttatc
      481 tatggttact cctacattta ttttctatca gtaaaaatca atactatcta caattagcaa
      541 acgtcaccgc taagaaatta atagagaatt atgatactct agaggaaata gactttgcat
      601 tgggaaaat
//
LOCUS       DAVMEF010000339          605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.343, whole genome shotgun sequence.
ACCESSION   DAVMEF010000339 DAVMEF010000000
VERSION     DAVMEF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.343"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..589)
                     /locus_tag="VYQ30_002116"
     CDS             complement(<1..589)
                     /locus_tag="VYQ30_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967145.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTA
                     WIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSY
                     LLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLN"
BASE COUNT          219 a           94 c           92 g          200 t
ORIGIN      
        1 aatttaaact tacaaagtaa ttgaatccca tagtatattt ttctttataa agcaaaggta
       61 aaactttata aaaaatatct aagtcaacct cactctgatt ctgaagcaag taggagtcaa
      121 gcaaattttc aaaaatagct atcaatatga ttggtgtaag gtttttgtat tcacatgtca
      181 agtattctga gtatcgtgca gatgatattt cttttatata actttttgaa agcaagattt
      241 tttcaaattc atcatctatt attaatcttc tatttccaat ccaagcggta ttgctaccca
      301 aataagattg tacaaatagc aatatgtaag aaaacatgaa cgtaaatact gaaagaggta
      361 gaattctatg cgtaaaaaaa tagacgataa tggtagccag gagtaaagct atctctcgtg
      421 acaatcgaat tcttttcagc atctttttta acagtccgta aggttgtcct tggcaatatt
      481 cttgaactaa attaatcgga atgacatctc tcaccatttc tggaaagtag agtaagttta
      541 taggctgaaa tctccagctc ccatcgtaag taaatggata tagaaccaaa tacttaaaag
      601 atttt
//
LOCUS       DAVMEF010000340          590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.344, whole genome shotgun sequence.
ACCESSION   DAVMEF010000340 DAVMEF010000000
VERSION     DAVMEF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.344"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            38..562
                     /locus_tag="VYQ30_002117"
     CDS             38..562
                     /locus_tag="VYQ30_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1967146.1"
                     /translation="MEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTL
                     FNFYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEA
                     ITKITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMS
                     MTLKVEHYIADITE"
BASE COUNT          211 a           80 c          106 g          193 t
ORIGIN      
        1 aaaagctcac cgcctatctt tagcagataa aatctacatg gaatggataa aatctctgat
       61 agatttttat ttttacggac agaaagaaga tgcgattgta cgattagagg gggtattatc
      121 acagttaagt gtatctgatt tgaactatct ccaagtttca aacaccctat tcaattttta
      181 ttatgatatt ggagatttag gtcgtttcaa taaaattcgt gaaaaactag agtatcaagt
      241 aaatcacctc aatttgagta caatcgaaga gttagaacta tctattaagt ttaactacaa
      301 cgtttgtcgt tatctatggt tacagaaaaa tattgaagaa gctattacta aaattacagc
      361 tactataaaa caatgtaagg cttacagaac gaattatctt ttggctgatt tgtacttatt
      421 gatgggaaat gtaagtgaaa atttttcttc taaaagttcg gtgaaagaat attttgagac
      481 ggcatatttt ctatacaaac ttgaggagaa catgtcaatg actctgaaag ttgagcatta
      541 cattgcagat ataacagaat aatgaatacg gtgttcaagt gagcatcgtt
//
LOCUS       DAVMEF010000341          590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.345, whole genome shotgun sequence.
ACCESSION   DAVMEF010000341 DAVMEF010000000
VERSION     DAVMEF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.345"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..125
                     /locus_tag="VYQ30_002118"
     CDS             <1..125
                     /locus_tag="VYQ30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1967147.1"
                     /translation="NVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFF"
BASE COUNT          213 a           88 c          103 g          186 t
ORIGIN      
        1 ttaatgtttc aggtaataaa gggcaaaaaa ataatattga attaattgcg aaacaaagaa
       61 gctttataga ttattgggct aaatacgttt cttatcttcc tttcccaacc ccagagtttt
      121 tttagctaat ttgtgtaaca gcgtagatag ggaaggttat gatttttcta aagatggcaa
      181 cggaaaagaa tattttagaa aaaaaactca agtagcgtta ggaatagaga atataacttc
      241 tgaagatatt ttccaggagc aaagaagagc tgtttcaaaa atacagccag aatcatcaat
      301 gtttcagtgt attaaagaaa aattagaggc attgttctaa ttccttagat taggtactac
      361 ataacaaatt ttttaaattc aaacccgcac caaaaacaac attgcaactt tcttctcagg
      421 taatgtgttg ggatttgaac agaccaacga gttcatagtg gtgtatgcca atgactttga
      481 taagtctacg cgtcagactt attagttcat ttatccagat atatctcgat aatcatatta
      541 tttagtcaaa gaacatccca tatgagggca tgaaatgcta agtttagctt
//
LOCUS       DAVMEF010000342          587 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.346, whole genome shotgun sequence.
ACCESSION   DAVMEF010000342 DAVMEF010000000
VERSION     DAVMEF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.346"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(12..362)
                     /gene="tnpB"
                     /locus_tag="VYQ30_002119"
     CDS             complement(12..362)
                     /gene="tnpB"
                     /locus_tag="VYQ30_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1967148.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDHFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(343..528)
                     /locus_tag="VYQ30_002120"
     CDS             complement(343..528)
                     /locus_tag="VYQ30_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967149.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
BASE COUNT          190 a          118 c          109 g          170 t
ORIGIN      
        1 aagtcctcat ttcaatagaa atcacgactt tctgataaat ttattttggg agtgatagaa
       61 aagcccttca taagccagtc tacttgttca ggtgtgagag ctttgacatc cttttctgta
      121 ctgggccaag tcagtctgcc gttctcaaag cgtttatata atagccaaaa tccttgacca
      181 tcccagtaaa gggctttaaa gtggtcttta cgtccaccac aaaagagaaa aacttgaccg
      241 gagaaaggat ccaattcaaa gtgggtttta accagataag ccagtgaatc aatgccttgc
      301 ctcatatccg ttttcccaca tacgagatag acctgcccta ggctagatag atgaattgtc
      361 atagagcaac accttatcca aaagctgttc tatcatttcg agattgagag attgaaaaaa
      421 acttacttca agcttgccaa tacgaatttt aagcagaata tcatttctct ttttagaatc
      481 aaaaggacga gattgtggaa tctctacagg aacaataggt tgtggcatta aaatatcctc
      541 caatagtttt actttactaa tagtacacag gaggagctcc atgaatg
//
LOCUS       DAVMEF010000343          565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.347, whole genome shotgun sequence.
ACCESSION   DAVMEF010000343 DAVMEF010000000
VERSION     DAVMEF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.347"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            176..520
                     /locus_tag="VYQ30_002121"
     CDS             176..520
                     /locus_tag="VYQ30_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967150.1"
                     /translation="MFRNGVNEKNFTEYVYKNFSQKDIALAENELETNINNPYDRVPW
                     DEMGGCIAGKIRDEFFAMINVSLIVKYAQKKAWSELAKVVLRFVKANGLKTNIYIIAG
                     QLAIWAVQCGLE"
BASE COUNT          196 a           68 c          108 g          193 t
ORIGIN      
        1 gaaatttaaa aaaattgtaa cttcagctct tgttgtatta tcactatttt ctattatgtc
       61 tccttctata gcttctgtta gagtatttgc tgatgatgta actactacgg aagtttctga
      121 aattagtacc caagaacaaa aacaaatcga tgatgtagcc aatgttcttg aacaaatgtt
      181 tagaaatgga gttaatgaaa aaaattttac tgaatatgtt tataaaaact tttctcaaaa
      241 agatattgct cttgcggaaa atgaattaga aactaatatt aataatccct atgacagagt
      301 cccttgggat gagatgggag gatgtatagc aggaaaaata cgagatgaat tttttgctat
      361 gatcaatgtt tcgctaattg ttaagtatgc gcagaaaaag gcttggtcag aattagctaa
      421 agtggtacta agatttgtta aagctaatgg acttaaaaca aatatttata tcattgctgg
      481 tcagttggct atttgggcag ttcagtgtgg tctggaataa tgcgtgataa aaacttttta
      541 ataaaagatg ggattattag ctttt
//
LOCUS       DAVMEF010000344          547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.348, whole genome shotgun sequence.
ACCESSION   DAVMEF010000344 DAVMEF010000000
VERSION     DAVMEF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.348"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..502
                     /locus_tag="VYQ30_002122"
     CDS             <1..502
                     /locus_tag="VYQ30_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1967151.1"
                     /translation="TGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFI
                     SSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFL
                     LFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEK
                     VNQLWK"
BASE COUNT          188 a           83 c           85 g          191 t
ORIGIN      
        1 tacaggtctt tcttacaaga agggcgattt aagcattaat aataaaggtg ttagaattat
       61 acgtggtggt aatattaagc ctttagaatt ttctctgttg gataacgatt actacattga
      121 tacacaattc atctcctctg agcaagttta tttaaaacat aatcagctaa taacacctgt
      181 atcaacctct ttagaacata ttggaaagtt tgcaagaatc gataaagact atgatggtgt
      241 tgtggctggt ggatttattt tccaattaac accattcgaa agttcagaga ttatttcaaa
      301 atttctatta tttaacttgt cttctccgtt attttataaa caattgaaag caataactaa
      361 actatcaggt caagctttat ataatattcc taaaactaca ctgagcgagc tattaattcc
      421 gttagctcct tttgaggaac aggaacttat tactcaaaaa gttgagaaac tttttgaaaa
      481 agtaaatcaa ctttggaaat gattcttttc atctcttcat gattagaaat agggattaat
      541 aattcgg
//
LOCUS       DAVMEF010000345          546 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.349, whole genome shotgun sequence.
ACCESSION   DAVMEF010000345 DAVMEF010000000
VERSION     DAVMEF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.349"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(61..>546)
                     /locus_tag="VYQ30_002123"
     CDS             complement(61..>546)
                     /locus_tag="VYQ30_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1967152.1"
                     /translation="NLGGGFMKLFMENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSA
                     ITSFSAYMITSPVNVSEVNEQQVITLDTSIYTNTLWFVPHIITTLNSSSKERYSTILY
                     GNTCYEHDKYKMKVSLPRLTQNSSIVFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLT
                     Y"
BASE COUNT          182 a           94 c           72 g          198 t
ORIGIN      
        1 ctttatattg attatccaga atttgagtag ttttatttcc taaatattcc ttaactttat
       61 ctaatatgtc aagttttttg tccacaaata tacctcccgc ataaaatcat tacgatgtaa
      121 atttgaatga ttgcttttta tataagctcc tacaggaaaa aacactatac tgctattttg
      181 agttaacctt ggaagcgaaa ctttcatttt atacttgtca tgttcataac aggtattacc
      241 atagagaata gtactataac gctctttact acttgaattt aacgttgtaa taatatgcgg
      301 aacaaaccat aatgtattgg tgtatattga tgtgtctaac gtgataacct gctgctcatt
      361 cacctcacta acattaactg ggctagtaat catataggca gaaaatgaag taattgcact
      421 acctggttct attattgtag tcacagtact atcaatatcg tactttttat agatttccat
      481 aagtgataga aaaaattctt tcaaacgatt ttccataaat aatttcataa aaccgcctcc
      541 aagatt
//
LOCUS       DAVMEF010000346          545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.350, whole genome shotgun sequence.
ACCESSION   DAVMEF010000346 DAVMEF010000000
VERSION     DAVMEF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.350"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..255
                     /locus_tag="VYQ30_002124"
     CDS             <1..255
                     /locus_tag="VYQ30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1967153.1"
                     /translation="KTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            330..527
                     /locus_tag="VYQ30_002125"
     CDS             330..527
                     /locus_tag="VYQ30_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1967154.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          189 a          103 c           72 g          181 t
ORIGIN      
        1 aaaactatcg ttttaagaat cccagttatt cctaatttta acaatagttt agaggatgca
       61 gaaaaattcg ctactctatt taactcatta aatatcgacc aagttcaact actccctttt
      121 catcaatttg gtgaaaacaa atatcgttta ttaaatcgga aatatgaaat ggatggagtc
      181 aacgcacttc atcctgaaga tcttattgat tatcaaaagg tatttctgaa ccaccatatt
      241 aattgttatt tctagttcat ttccttgaaa tgctctagct atttgcagat aacaagcatc
      301 tatataaggt tatcaaagaa gacttcccca tgactctaaa tgaatatatc ttacaatatc
      361 gcctgaaaca agctatagat aagatggctg aatctcccaa ctccccttta agcgctatct
      421 ctgaccaagt tggattttca gactataaat attttgccaa agtatttaaa aagtatctcc
      481 atatttcccc aaagaaattg aaatcactcg gaagaatagt caaataggct attcttcttt
      541 ttgta
//
LOCUS       DAVMEF010000347          543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.351, whole genome shotgun sequence.
ACCESSION   DAVMEF010000347 DAVMEF010000000
VERSION     DAVMEF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.351"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            28..>543
                     /locus_tag="VYQ30_002126"
     CDS             28..>543
                     /locus_tag="VYQ30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967155.1"
                     /translation="MTQSINKKVKTYSLSTKQKLALLLTLVTKPDILILDEPTNGLDI
                     ESSQIVLAVLKKLALNENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNF
                     LFEIAFSSATDRDIFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFE
                     TKKETLKDIYLN"
BASE COUNT          200 a           63 c           97 g          183 t
ORIGIN      
        1 tttagaagtt tgatccaaga gttagatttg actcagtcta ttaataaaaa agtaaagacc
       61 tattctttga gtacaaaaca aaaattagct ttgcttctaa ctctcgttac gaaacctgat
      121 atattgattt tagatgaacc gactaatggt ttagatattg aatcatcaca aatagtttta
      181 gcggttctaa aaaaattagc tttaaatgaa aacgtgggaa ttttaatatc gagtcataaa
      241 ttagaagaca ttgaagaaat ttgtgagaga gttcttttct tggagaacgg gcttttgaca
      301 tttcaaaaag taggaaaaga tagtcataat ttcttgtttg agatagcttt ttcatcagct
      361 acagatagag acattttcat taccaaacaa gaatttgggg atattgttca ggaagaggga
      421 ctgagaatta ctatgtctgg gaatattcaa agtagtgagc tttttaaatt ttttaacgaa
      481 aactctatta aagtagttga ttttgaaact aaaaaagaga cgcttaaaga tatttactta
      541 aat
//
LOCUS       DAVMEF010000348          530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.352, whole genome shotgun sequence.
ACCESSION   DAVMEF010000348 DAVMEF010000000
VERSION     DAVMEF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.352"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>530
                     /locus_tag="VYQ30_002127"
     rRNA            <1..>530
                     /locus_tag="VYQ30_002127"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          160 a           96 c          160 g          114 t
ORIGIN      
        1 cagactgcga gtgctaagat ccgtagtcga aagggaaaca gcccagacca ccagctaagg
       61 tcccaaaata attgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttag
      121 cttagaagca gctattcatt caaagagtgc gtaatagctc actagtcgag tgaccctgcg
      181 ccgaaaatgt accggggcta aaacaattta ccgaagctgt ggataccttt ataggtatgg
      241 taggagagcg ttctatgtgt gatgaaggta taccgtgagg agtgctggaa cgcatagaag
      301 tgagaatgcc ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg taagactaag
      361 gtttccaggg gaaggctcgt ccgccctggg ttagtcggga cctaaggaga gaccgaaagg
      421 tgtatccgat ggacaacagg ttgatattcc tgtactagag tatgtagtga tggagggacg
      481 cagtaggcta actaaagcag acgattggaa gagtctgtct aagcagtgag
//
LOCUS       DAVMEF010000349          530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.354, whole genome shotgun sequence.
ACCESSION   DAVMEF010000349 DAVMEF010000000
VERSION     DAVMEF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.354"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>530
                     /locus_tag="VYQ30_002128"
     CDS             <1..>530
                     /locus_tag="VYQ30_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic transporter"
                     /protein_id="HEV1967156.1"
                     /translation="SDTLGQFENGLFYPISNRIVKKSDREETMAFRQTATSTMNIVNQ
                     SLGAFLITFLSFFHLALINSLTFAISLLITLAIKSQINNFYIDKTPSTKVSKVDFKAT
                     FSDIISNLKLSLKHLFSLTNMKTVLLVIPILNGSLAIIIPLAVVNLSKSSALTIISSA
                     TTISVLGISTVSGGIL"
BASE COUNT          169 a           92 c           75 g          194 t
ORIGIN      
        1 tttcagatac attggggcaa tttgaaaatg gtcttttcta tccaatttct aatcgaatag
       61 tgaaaaaatc agatagggaa gaaaccatgg cgtttaggca aacagcaact tctaccatga
      121 acattgtaaa tcagtcgtta ggggcatttt taatcacttt tcttagtttc tttcacttag
      181 ctcttataaa ttctctaaca ttcgctatta gtttactaat aacattagct atcaaaagcc
      241 aaattaacaa tttttatata gacaagaccc catccactaa ggtttcaaaa gttgatttta
      301 aagctacgtt ttctgatatt atttcaaact taaaactttc gcttaaacat ctatttagtc
      361 tcactaatat gaagactgta ttacttgtta ttccaattct taatgggagc ttagccatta
      421 tcataccact agctgtagtg aatctttcta aaagttctgc tcttacaatt attagttctg
      481 caacaacaat ttcagttttg ggaattagta ctgtttctgg agggatttta
//
LOCUS       DAVMEF010000350          525 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.355, whole genome shotgun sequence.
ACCESSION   DAVMEF010000350 DAVMEF010000000
VERSION     DAVMEF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.355"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>525)
                     /locus_tag="VYQ30_002129"
     CDS             complement(<1..>525)
                     /locus_tag="VYQ30_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HEV1967157.1"
                     /translation="LYPNLDRFAVNQFELYCEMKNFGEQPVISIVPNTGSDMIGKSIG
                     RFASYFLNSNIELDSRVDNVELIEFPSDNKNLNVMSSHHGHSKKLLLSYEDDFDIDSL
                     ELDFLVVGVERVNEHYKLYFRDLRTDLIVNFVTTSMLNHKSIGVFSHLARFLLTVSLE
                     WQDNPFSLFRVIEN"
BASE COUNT          194 a          101 c           59 g          171 t
ORIGIN      
        1 gattctcaat taccctaaat agagaaaatg gattatcttg ccactctaac gaaactgtta
       61 acaaaaatct cgctaaatgg ctaaaaactc cgatagattt atgattcagc attgaggttg
      121 ttacaaaatt aacaatcaag tctgttcgta agtctctaaa atatagtttg taatgctcat
      181 tcactctctc aacacccact actaagaaat ctaattctaa cgagtcaata tcaaagtcat
      241 cttcataaga tagtaataac tttttagaat gtccatggtg agaagacata acatttaaat
      301 ttttattatc acttggaaat tctattaatt ctacattatc aaccctcgaa tctaattcta
      361 tattagaatt tagaaaatat gaagcaaatc taccaataga ctttcctatc atatcactcc
      421 cagtatttgg cacaattgat ataacaggtt gttctccaaa attcttcatt tcacaataca
      481 actcgaactg attcacagca aatctatcca aatttggata caata
//
LOCUS       DAVMEF010000351          524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.356, whole genome shotgun sequence.
ACCESSION   DAVMEF010000351 DAVMEF010000000
VERSION     DAVMEF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.356"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..447
                     /locus_tag="VYQ30_002130"
                     /pseudo
     CDS             1..447
                     /locus_tag="VYQ30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
BASE COUNT          178 a           68 c          100 g          178 t
ORIGIN      
        1 tattcttgaa aatatttttg atttattgac tattctccct ttaaatgcta tttttacagt
       61 gtttcgatta ggacgtatct tccgcttagc aaggctgaca aaattactga aactgactcg
      121 tttacttaga ataattggac ttacaggtaa attagaaaga aaaattagta gattcttacg
      181 aacaaatgga ttgatttata tcttgtatgt taatatcttt attgttctag tagggagttc
      241 gattttatcc gttgttgaag aaaaatcctt ctcagatagt ctttggtggg ctcttgtgac
      301 agtaactact gttggttatg gtgatattgt tccagtttcc ctttttggga agacgaatta
      361 tcgagccaaa gaatattaat agagaaacaa tccgagaaga tagaagaact gcatcggatg
      421 atacaagatt tgcttgaaaa aacataataa aaataaagat gagacaaagt ttgagttgag
      481 gagaaatcaa gtaagttttg acacaataga acgcatatta tcag
//
LOCUS       DAVMEF010000352          521 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.357, whole genome shotgun sequence.
ACCESSION   DAVMEF010000352 DAVMEF010000000
VERSION     DAVMEF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.357"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..260)
                     /locus_tag="VYQ30_002131"
     CDS             complement(<1..260)
                     /locus_tag="VYQ30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967158.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFIT"
BASE COUNT          144 a          118 c           51 g          208 t
ORIGIN      
        1 tttgtaataa atgtctgtgc taatttctta aattctgata actcctcaac tttctctaca
       61 acctgctcat caaaaaaata atccatactg acttttaact tcttagcaag ggcatgcaag
      121 aaatctgctc caggagtaaa ctctccgctc tctaatcgac tgatttgccc ctgtttacag
      181 ataccttcag ctagttctcg ctgagacatt tttaattctt ttcgtctatt ttttaatctt
      241 gttgctaata gtgtgcccac gtttgcatct tcctcttcta tttgatttca tttttaattg
      301 taacatgatt aaatctaaaa ttcaaatcaa tgatttgaaa gatacaaatt gtatataaaa
      361 tctatttttg caattcatca tcaacttatt tacttttcat ttcttcattc aaactcttta
      421 taaacttatc cagcttcaac gccacttttc atacctatca ccttctaatt acaactgttc
      481 ccctagttct ttcagcccct ttctgtatct attttcctag a
//
LOCUS       DAVMEF010000353          516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.358, whole genome shotgun sequence.
ACCESSION   DAVMEF010000353 DAVMEF010000000
VERSION     DAVMEF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.358"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..483
                     /locus_tag="VYQ30_002132"
     CDS             <1..483
                     /locus_tag="VYQ30_002132"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1967159.1"
                     /translation="ENYPKEGITFRDISPLMADGNAYSYAVREIVQYATDKKVDMIVG
                     PEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLTMHADAIKPGQ
                     RVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIGDYDYKVLMHY
                     "
BASE COUNT          134 a           92 c          132 g          158 t
ORIGIN      
        1 gaaaattatc caaaggaagg cattaccttc cgtgatatta gtcctttgat ggctgatgga
       61 aatgcttata gctacgctgt tcgtgaaatc gttcagtatg ctactgacaa gaaagtcgac
      121 atgatcgtgg gacctgaagc tcgtggattt atcgtgggtt gtccagttgc ctttgagttg
      181 ggaattggtt ttgcgcctgt tcgtaagcca ggtaaattgc cacgcgaagt tatttctgct
      241 gactatgaaa aagagtacgg tgtcgatacc ttgactatgc acgcggatgc cattaagcca
      301 ggtcaacgtg ttcttattgt agatgacctt ttggcgacag gtggaactgt taaggcaact
      361 atcgagatga ttgaaaaact tggtggtgtt atggcaggtt gtgccttcct tgttgaattg
      421 gatgaattga acggccgtga aaaaattggt gactacgact acaaagttct tatgcattat
      481 taatgaaaac agtccctagg gctgttttct ctacac
//
LOCUS       DAVMEF010000354          514 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.359, whole genome shotgun sequence.
ACCESSION   DAVMEF010000354 DAVMEF010000000
VERSION     DAVMEF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.359"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>514
                     /locus_tag="VYQ30_002133"
     CDS             <1..>514
                     /locus_tag="VYQ30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967160.1"
                     /translation="KVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGS
                     NGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNE
                     LRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDE
                     PSSGMDYSNMI"
BASE COUNT          199 a           63 c          101 g          151 t
ORIGIN      
        1 aaagttcctt atttaaaaga aggtggagag tatcagataa aaaatcttag ctacaaattt
       61 actgatgatg agtgtttaag cttaaaagat atttcattca agcttgggaa aatttatggc
      121 ataataggat ccaacggacg aggaaaatca acgcttttaa gatgtttaat aggtcttgag
      181 aaaaaatcaa aagaagaaat ttattttaag ggagagaagc tatctaaaaa agaaagactc
      241 aaaaactctt cacttgttat gcaagatgta aatcatcaat tattcacaga tgaagtattc
      301 aacgagctta gattaggagt aaagaatttt gatgaagaaa aggcgaaaat cattttaaaa
      361 gatttaggcc tggacgaatt tattgaaagg catccgatga gtttatcagg agggcaaaag
      421 caaaggcttg caatagcatc tgttatgtgc aagaattctc catttatctt ttttgacgaa
      481 ccttcaagtg gtatggatta ttccaatatg ataa
//
LOCUS       DAVMEF010000355          502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.360, whole genome shotgun sequence.
ACCESSION   DAVMEF010000355 DAVMEF010000000
VERSION     DAVMEF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.360"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..116
                     /locus_tag="VYQ30_002134"
     CDS             <1..116
                     /locus_tag="VYQ30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1967161.1"
                     /translation="FKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
BASE COUNT          163 a           64 c           86 g          189 t
ORIGIN      
        1 attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat atggatgact
       61 taattagaaa aatattttca aaatcttcca aaaaggatac ggatagtact ctctaaaaaa
      121 taaatttctt ccctgtattt gctagcttaa tttacagggg agtttcttta atattgtttc
      181 gcctgtcaaa atgatatgtg atagcaggat gaaaagtcta caaacaagtt ggtatcaata
      241 caacataaag aggaaagtat gtttttcatg cttgcttttt ttaattttta caattcaaga
      301 ggttttgatg accatttatg atttgagtga tccaaggtca aaataagtgc tatactagca
      361 gtgtaaatgt tcttgctcaa ctaagataaa tcgcacattt aggaggaaat cttatgaaga
      421 aaaaaatact gattattttc gttctgtatc tgatcatgtc catctttctt tatccgctta
      481 gggagagtat ttggtataat ct
//
LOCUS       DAVMEF010000356          502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.361, whole genome shotgun sequence.
ACCESSION   DAVMEF010000356 DAVMEF010000000
VERSION     DAVMEF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.361"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(85..>502)
                     /locus_tag="VYQ30_002135"
     CDS             complement(85..>502)
                     /locus_tag="VYQ30_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1967162.1"
                     /translation="KNKVDQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
BASE COUNT          124 a          104 c           82 g          192 t
ORIGIN      
        1 ttgtcaagtt tctgttacgt ttttctaaaa aagagaagta acacgaaagg ttacttctcc
       61 ataaaatatg ctccattcat tttactactg tataggagcc ggttgacttt cttttaacaa
      121 agccaagaga ctatctactt ctgctgaaat agtgttatta tctttcgttc cgagaagaag
      181 actacttttt agaccagtca atgtctccat agcattttgt ctaatactag gatctgttac
      241 tttttctagc aaggcctccg catctgctat cttagcgtta ataacagaat tctctacttg
      301 aggaatttca gcctcatctg tggtatcttc agcttcttcc tctgtctctt ccgtatcagt
      361 gcttggttta taaagattat ctgctgaagg atttaaacca gcgtgattct ctttttcatc
      421 agattcaggg tgagttggct cacttacttc atcatgttcc ttatcttcat caggtttact
      481 gtcttggtct accttatttt ta
//
LOCUS       DAVMEF010000357          494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.362, whole genome shotgun sequence.
ACCESSION   DAVMEF010000357 DAVMEF010000000
VERSION     DAVMEF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.362"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(137..>494)
                     /locus_tag="VYQ30_002136"
     CDS             complement(137..>494)
                     /locus_tag="VYQ30_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1967163.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNT
                     IMAEAEKLLALLKESK"
BASE COUNT           94 a          104 c           83 g          213 t
ORIGIN      
        1 tactattcta gtttcaatct actatatttt gcacattttc atgaaaaaaa tgagaactag
       61 aactcacatt ctgctctcat ttttcgtttt cccgttctcc tatcctgttt ttaggagtta
      121 gaaaatgctg ctacctttac ttactctcct ttaataaagc caatagtttt tcagcttctg
      181 ccataatagt attgttgtcc tgggtgccaa atagtaaatt attttttaat cctgtgagag
      241 tctctttggc attggacttg ataattggat cctggatttt tccaagtaaa tcttcagcct
      301 ctctcagttt ttcttcaacc ttttcagtct cgacctgagg ttcttctgat tcctctgttg
      361 gttttggaga ctctggtttc tcgctttgcg gtttctcttc tcgaggggtt tcttcctcag
      421 gtttttctgt ctgaggtttc tcctcgcttg gtttttccgt ttgattggta tcagcttgac
      481 catttttgtt tctt
//
LOCUS       DAVMEF010000358          493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.363, whole genome shotgun sequence.
ACCESSION   DAVMEF010000358 DAVMEF010000000
VERSION     DAVMEF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.363"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            54..263
                     /locus_tag="VYQ30_002137"
                     /pseudo
     CDS             54..263
                     /locus_tag="VYQ30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           56 c           95 g          164 t
ORIGIN      
        1 ataatgattc aaacaatttt actaaagaac aagtattaaa gaattgggac tctatgctac
       61 gtgaatctat attaagagtg tatagtgaag caggagaggc taatattaca ctatcatctg
      121 gttttgattc aaattatata ttatatacat tagcgaatta tacgaattct tcaattaatg
      181 cattttgcat aggaggagaa aaaggaatta atgagattcc tgaagtaact aaaattgcaa
      241 aattttatgg tataaattta ttagttgata ctgtaatgag ccaagattta gaatattttc
      301 ccgatatagt atggagattg gaaggaagtc tctttgaatg tggtgtcata ctgcagtact
      361 atttaggcag gttgttgttc caaaaaggga acacaagtat tctttgtggc gagtcagcgg
      421 atgaaataat gacttttaag tatcattctg tgaactacaa tcaattttgt aatgataaac
      481 agaagtcagt ata
//
LOCUS       DAVMEF010000359          492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.364, whole genome shotgun sequence.
ACCESSION   DAVMEF010000359 DAVMEF010000000
VERSION     DAVMEF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.364"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            140..448
                     /locus_tag="VYQ30_002138"
                     /pseudo
     CDS             140..448
                     /locus_tag="VYQ30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          198 a           58 c           97 g          139 t
ORIGIN      
        1 atatttgcta aactttcaga aaaaaagttg gagtatatga tgctaggtga ctgcgtcatg
       61 ttagtaaacg aaatggaaat aactgatcat agagtagata acttatttga aaaagggaaa
      121 aatgagataa aagattcaag tagtagattt agcaatagga catataaaag ctttagaaaa
      181 agtatctgaa aaaacagatg tttatatcta taacctaggc tcgggagaag gcactagtgt
      241 attacaactt gtaaatacat ttgaaagtgt tagtaagatc cctattcctt ataaaatagt
      301 tccaagacgt tcaggagacg ttgcaactta ttatgcaaat gcagacaaag catataagga
      361 attaaattgg aggacaacaa aatcgattga agacatgtgt agagatacat ggaattggca
      421 atcaaaaaat cccaatggct ataattgatg atactgtcta tatgagtgct gtattttgga
      481 aaaattactt aa
//
LOCUS       DAVMEF010000360          491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.365, whole genome shotgun sequence.
ACCESSION   DAVMEF010000360 DAVMEF010000000
VERSION     DAVMEF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.365"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            19..>491
                     /locus_tag="VYQ30_002139"
     CDS             19..>491
                     /locus_tag="VYQ30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1967164.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQT"
BASE COUNT          177 a           54 c           95 g          165 t
ORIGIN      
        1 tcgtaatgga ggactgaaat gaaaaatata aatgggaaaa aggtaactat ttatgatatt
       61 gcacgtttat caggtttttc tccaaaaaca gtttcacgag ttattaatgg tggagtaaat
      121 gttaaagaag aaacttatca agcgattcaa aaagttatag aggaattgtc gtatattcca
      181 aatgcgtatg caaaaaattt aactaaaaaa gaagctataa atatattgat ttctgtaaaa
      241 aaaatcgact cttttccttt aatttggttt catacgctgt tagataaagt gttacggacg
      301 tgtaaagaat ttggtgttaa tgctatagtt gagtactttg gagaggagga tacaattagt
      361 aattcaatta tatcaagcac aggtagccta gtagatggtg ttattgtttt ttatgaaagt
      421 gtagatgata tcagaattca gtatttaaag aagaaccata tgccttttct tgttttcggt
      481 gaatctcaaa c
//
LOCUS       DAVMEF010000361          487 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.366, whole genome shotgun sequence.
ACCESSION   DAVMEF010000361 DAVMEF010000000
VERSION     DAVMEF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.366"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>487
                     /locus_tag="VYQ30_002140"
     CDS             <1..>487
                     /locus_tag="VYQ30_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1967165.1"
                     /translation="DKRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFI
                     KELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRY
                     RQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEY
                     KN"
BASE COUNT          218 a           66 c           98 g          105 t
ORIGIN      
        1 gacaaaagga taaaaaggtt aaggagaata tctggaggag atacctatac gataatctac
       61 ctaaaacttc tactactaag cctaaaagat gagggcaaac tctattatga cggagtagaa
      121 agtgatttta taaaagagtt agcactaacc atagatgaaa cagacgatga tgtaatggta
      181 acagtaaatt atttaatcaa tcaaggctta ttagaagttg taacagaaaa tgatgaatac
      241 tacctaactg aaataccaaa cttaatcgga tcagaaacag cctgggcaga gaaaaaaaga
      301 agatacagac aaaataaaca gaggacattg tccttaatga gtccaaccca tgtccgacaa
      361 gagatagaga tagagaaaga tatagagata gataaagaga gagagggaga gatagataaa
      421 aagtccaccc ccatttttta tggagaattc aaaaatgtaa ggttaagcaa agaagaatat
      481 aaaaatc
//
LOCUS       DAVMEF010000362          476 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.367, whole genome shotgun sequence.
ACCESSION   DAVMEF010000362 DAVMEF010000000
VERSION     DAVMEF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.367"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            75..476
                     /locus_tag="VYQ30_002141"
                     /pseudo
     CDS             75..476
                     /locus_tag="VYQ30_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442116.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          158 a           53 c           91 g          174 t
ORIGIN      
        1 aactgactta ctcaattagt gttgttttgt tacatcaagg agataaaaat agtttattag
       61 agtctttgtc aatagtggag agacaaacat taaaagatat agaagttatt attgtaaatc
      121 aatctacaga ggagatagct tccattagcc aacagatgaa tctatcattt aatattaaga
      181 ttatagatga gaattcttgt gctgagttgt ctgatatgat tacaggagat tatattacgt
      241 ttctatcttc aaatgattca ttatttgatt ggacatttga aaagatgtat catgcgataa
      301 aattatccga ttctgatgtt gttcttggtc attttgctga tttggttgat ggagtatttt
      361 acttttatcc ttgggggggg ataattggtt gacagaaaat ttaacgaatg ttcaagtttt
      421 acaaaagatg gatgatacag atcatcgaat tagaaaacaa tatcgctcat tatttg
//
LOCUS       DAVMEF010000363          468 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.368, whole genome shotgun sequence.
ACCESSION   DAVMEF010000363 DAVMEF010000000
VERSION     DAVMEF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.368"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>468
                     /locus_tag="VYQ30_002142"
     CDS             <1..>468
                     /locus_tag="VYQ30_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1967166.1"
                     /translation="KSIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRH
                     QIIRLIPNKTAESVNQALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSS
                     WERGSNENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFL"
BASE COUNT          156 a           88 c           90 g          134 t
ORIGIN      
        1 taaatctatc gaagaacgtc ctgacgttat taatcttcgc ttggaaaatg gtcattatga
       61 aattgatacc gtcctactga ctaagataaa aaattattgc ctgttagtct taaccgaccg
      121 gcggagcaga caccaaatta taaggttaat tccaaataaa actgctgaat ctgtcaatca
      181 ggcgcttacg ttactattag gggagcatcg tattctgtcc attactgcag ataatggttc
      241 ggagttcaaa cgattgtctg aggtatttcc tgaggaacat atctactacg cacatgctta
      301 ctcttcatgg gagagaggtt caaatgaaaa tcataatcga ttaattcgga gatggttacc
      361 taaaggaacc aagaaaacga ctccgaaaga agtagctttt atcgaaaatt ggattaacaa
      421 ctaccctaaa aaatgcttgg actacaagtc gccaagtgaa tttctttt
//
LOCUS       DAVMEF010000364          467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.369, whole genome shotgun sequence.
ACCESSION   DAVMEF010000364 DAVMEF010000000
VERSION     DAVMEF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.369"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..>467
                     /locus_tag="VYQ30_002143"
     CDS             17..>467
                     /locus_tag="VYQ30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1967167.1"
                     /translation="MKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVYSY
                     GIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDG
                     VVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYN"
BASE COUNT          171 a           52 c           97 g          147 t
ORIGIN      
        1 tgccagaaac ataggtatca aaaattctag aggtaagtat atatcatttt tagacgatga
       61 tgatgaatat atgccagatc gaattttaaa gttgatggct tgttttaaaa agagtagaat
      121 gaagaattta gctttagttt atagttatgg aataataatt tatccaaatg gtacacgaga
      181 agaggagaag accgattttg ttggaaatcc cttgtttgtt caaatggttc acaatatagc
      241 aggtacgtca ttttggttgt gtaaaaaaga ggtgctagaa ttaattaatg gttttgagaa
      301 aatagattca catcaggacg gtgttgtttt attaaaacta cttgctcaag gataccaaat
      361 tgatatagtg cgagaattct tggtgaatta ctacgctcac agtaaagaga acggtatcac
      421 tggagtgaca caaaaaacaa ttaatgcaga tgaagaatat tataatt
//
LOCUS       DAVMEF010000365          463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.370, whole genome shotgun sequence.
ACCESSION   DAVMEF010000365 DAVMEF010000000
VERSION     DAVMEF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.370"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..460)
                     /locus_tag="VYQ30_002144"
     CDS             complement(<1..460)
                     /locus_tag="VYQ30_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1967168.1"
                     /translation="MRTFKTGIAVFLVLLIFGFFGWKGLQIGALTAVFSLRESFDESV
                     HFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPICTMLTIMTNVAMNNKAGV
                     IGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVNYDIDRIRLFL"
BASE COUNT          176 a          106 c           75 g          106 t
ORIGIN      
        1 ctaaaaagag acgaatacga tcaatatcgt aatttacgat aattgcgaca aaaactccca
       61 taaacgtttc tgatacacgc acaaacacgt acgaaattgt ctcaccactt ggaattgata
      121 gggtaatgat taacatagct gctacaccac caataacccc tgctttgtta ttcatggcta
      181 catttgtcat aatggttaac atggtgcaga ttggaacaac taccaaggtc acccaaaagg
      241 cttcatggaa aaaggtattt aataagaaga agaccaaggc atagagtcca ccgatactat
      301 ttcctagaat acgcgaagtc ccaaaatgaa cactctcatc aaaactctcc ctcaggctaa
      361 aaacggctgt caaagcacca atttgaagac ctttccagcc aaaaaagcca aaaatcaaga
      421 gaactagaaa aacagcaata cctgttttaa aggttcgcat acc
//
LOCUS       DAVMEF010000366          463 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.371, whole genome shotgun sequence.
ACCESSION   DAVMEF010000366 DAVMEF010000000
VERSION     DAVMEF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.371"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..>463
                     /locus_tag="VYQ30_002145"
     CDS             22..>463
                     /locus_tag="VYQ30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967169.1"
                     /translation="MTSLSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSV
                     LYAALSTIVSVAVCSMYAYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGL
                     LNTAWALVLPGAVSTYYIILFRTQFKSIPMELRESARIDGASEFR"
BASE COUNT          131 a           69 c           91 g          172 t
ORIGIN      
        1 tatccattat ggtgggtagt gatgacgtca ctttcagaac cagtttggct atctaaaaac
       61 gttgttaaat tatttccctt aaaaatagat attactgcat ataagtttat ttttggggat
      121 tctaaactat ggcaagcata tggtaatagt gtattgtatg ctgcactatc tacaattgtt
      181 agtgttgcgg tatgtagtat gtatgcctat ccacttacaa ttcctgaaat taggggtaaa
      241 aaattctgga atactcttat tttgattcca atgttcttta gtggaggaat gatcccgctt
      301 tatatacaag ttgcaaacct cggtttattg aatactgctt gggctcttgt tttacctggt
      361 gctgtttcta catattatat aattttgttt agaacacagt ttaaatcaat cccaatggag
      421 cttcgtgagt ctgctagaat tgatggggca agtgaattta gaa
//
LOCUS       DAVMEF010000367          460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.372, whole genome shotgun sequence.
ACCESSION   DAVMEF010000367 DAVMEF010000000
VERSION     DAVMEF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.372"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>460)
                     /locus_tag="VYQ30_002146"
     CDS             complement(<1..>460)
                     /locus_tag="VYQ30_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1967170.1"
                     /translation="DYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSNKL
                     KRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANI
                     KNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGV"
BASE COUNT          160 a           89 c           57 g          154 t
ORIGIN      
        1 caaacacctt ttattacaac ttcagcattt aaacttctcc tctcagcaaa agaatctagt
       61 tcattaaaaa aagcaacacg gagagctaag aatgtgttag aaaaaagctt aattgcttct
      121 gcttcagtag gagaaactaa cataacattt ttaatattgg cagtactatg agtactaatc
      181 gaaaggaaca actctgcaat ttttcttcct tcaactgtct catctccaac aactatgcga
      241 cttggatata aattatcata tatagaacaa ccttctctca aaaattcagg gacaaaaatg
      301 atattttttg tatcaaacaa cctttttaat ttgtttgaaa agccgatcgg aactgttgac
      361 tttaaaataa tctttccatt aggttttacc ctcagaatct tcgataccgt ttgttcgatt
      421 tcatatgtat taaaactacc aattttctca tcataatctg
//
LOCUS       DAVMEF010000368          442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.373, whole genome shotgun sequence.
ACCESSION   DAVMEF010000368 DAVMEF010000000
VERSION     DAVMEF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.373"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>442)
                     /locus_tag="VYQ30_002147"
     CDS             complement(<1..>442)
                     /locus_tag="VYQ30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967171.1"
                     /translation="NPSYLKSIQTSMSMGVTLISGFILYTQNMYSFMNTANGVNEARA
                     SFIAYLSAPIILIITSSISLTILSSNKDIEDIKQLKTLGVSRLQLFKIRIGEAIIHSV
                     LILLVSAIFNLIILILVSFIGQFLGHSLVDISGFWQPSLIVISLL"
BASE COUNT          168 a           71 c           67 g          136 t
ORIGIN      
        1 ctaacaaaga aataactata agactcggtt gccagaaacc tgatatatcc actaaactat
       61 ggcctaaaaa ttggccaata aagctaacta aaattaaaat aattaaattg aaaattgctg
      121 acactaataa aattaacacc gaatgtatta ttgcctcgcc tatacgtatt ttaaaaagtt
      181 gtaatcttga cactcccagt gtctttaact gtttaatgtc ctcaatatct ttattagaag
      241 ataaaattgt aagagaaata gaactagtaa taatcagaat aattggagca gacagataag
      301 caataaagga agctcttgct tcgtttactc cgtttgctgt attcatgaat gaatacatat
      361 tttgggtgta aagaataaac cccgaaatga gagttactcc catgctcatt gaagtttgta
      421 tacttttcaa atagctagga tt
//
LOCUS       DAVMEF010000369          431 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.375, whole genome shotgun sequence.
ACCESSION   DAVMEF010000369 DAVMEF010000000
VERSION     DAVMEF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.375"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..237
                     /locus_tag="VYQ30_002148"
                     /pseudo
     CDS             20..237
                     /locus_tag="VYQ30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          179 a           49 c           84 g          119 t
ORIGIN      
        1 agaaaaaaag agggatatta tggcacttaa ctataaacca ttatggatac agttagcaaa
       61 aaaagactaa agaaaacaga tgtaatagct atggcaggac ttacaacaaa tgttatggca
      121 caaatgggaa aggataaacc aattacattt aagaatttag aaagaatatg taaggcttta
      181 tcttgcactc ctaatgatat tattagtttt gaagataatt ttagtgacga ggaatagaaa
      241 atgactttaa ggacagaaga tcaagttagg gattatgcaa gagaagtata ggctttaatg
      301 aagttgaaga aaacatcaat caaggtactg gtcaaataac tacttttaat caattaggct
      361 tcaagggata ttcagataag ccagatggtt ggtatttacc taaaaatatg aatgatgtag
      421 caataatcct t
//
LOCUS       DAVMEF010000370          428 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.376, whole genome shotgun sequence.
ACCESSION   DAVMEF010000370 DAVMEF010000000
VERSION     DAVMEF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.376"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..208)
                     /locus_tag="VYQ30_002149"
     CDS             complement(<1..208)
                     /locus_tag="VYQ30_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967172.1"
                     /translation="MFYNQQKNRMIYVLSNFLYAISVSIIYAWNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLL"
BASE COUNT          172 a           74 c           62 g          120 t
ORIGIN      
        1 ataatagaaa ataaaacagg actaacaaaa ttgtattgac tacaatagct actataaaat
       61 ctaatcctaa atcacctgga atacccaatt tacttactat gactagtaac acaatgccat
      121 tccaagcata aataatggaa actgagatgg catatagaaa attagacaag acgtaaatca
      181 tccgattctt ctgttgatta taaaacaaac ttaccgtatt gtgggaaaaa tctctactaa
      241 tgactcgatt agcatgtata acagccatta acataccgag catagcataa aagtttgaaa
      301 tatgcttaat accacgcgat gtcccttgag tctttagaat aaagaatgta agcaatatcg
      361 gcggtataaa cgagataagc aaactgagat atattacgcc tgatgaatat aaatttctta
      421 tattttcc
//
LOCUS       DAVMEF010000371          427 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.377, whole genome shotgun sequence.
ACCESSION   DAVMEF010000371 DAVMEF010000000
VERSION     DAVMEF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.377"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..427
                     /locus_tag="VYQ30_002150"
                     /pseudo
     CDS             7..427
                     /locus_tag="VYQ30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826702.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT          138 a           74 c           85 g          130 t
ORIGIN      
        1 aaccttgtgt tttgcttaga agcggataat caagaataac tttttgatca ggtagcaagc
       61 ttgttggaag aacgagaaat tgtcactcca acttatcgtg aagccttgat cacgcgtgaa
      121 aagtcatttc caactggttt agatatggaa tttctaggaa aggacttgca aatgtaatga
      181 ttcctcacac agatattgtt cataatctag ctgagaaagt ggtggttgtt cgattagaga
      241 aaccggtgac tttccataat atgattgcgc caggtaagga agtagaagta tccttgctct
      301 tctttatcat caataactca agttcaagtc aaacaaatat tctggctcag ttgatggact
      361 ttttcacagg aaatggacat cttgaagacc tatcaaaaat ttccgaacca gaaaaacttt
      421 atgctta
//
LOCUS       DAVMEF010000372          424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.378, whole genome shotgun sequence.
ACCESSION   DAVMEF010000372 DAVMEF010000000
VERSION     DAVMEF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.378"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(82..>424)
                     /locus_tag="VYQ30_002151"
     CDS             complement(82..>424)
                     /locus_tag="VYQ30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1967173.1"
                     /translation="PLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYMKEHLLQVAEA
                     LQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRYKKMSFTWYKG
                     VIESNGESLFK"
BASE COUNT          137 a           87 c           54 g          146 t
ORIGIN      
        1 ttaatgtctt ccaaacttct ttattccata tttaatgaaa tgccacctta accgtgataa
       61 tagctagtca tcaataaaaa actatttgaa taaggattct ccatttgatt caatcactcc
      121 tttataccaa gtaaaggaca ttttcttata tcgattgaat gtgccatttc catcatcatt
      181 tcgatcaaca taaatgagac cgtacctctt agaaagctgt gcagtggaca tagaaacgca
      241 gtcaatacat ccccaagacg tatagcccat aatttccaca ccatcttgta gagcttcagc
      301 aacttgcaat aaatgttctt tcatatactg aattctataa tcatcttgga cggttaagtt
      361 attaagttca tcttttatta gttgatcttt agcacctaat ccattttcta ctataaataa
      421 tggg
//
LOCUS       DAVMEF010000373          423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.379, whole genome shotgun sequence.
ACCESSION   DAVMEF010000373 DAVMEF010000000
VERSION     DAVMEF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.379"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..423)
                     /locus_tag="VYQ30_002152"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="VYQ30_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          137 a           90 c           82 g          114 t
ORIGIN      
        1 aactttcgat ccaattgatt catgatttga acacgcacac gactcatagc acggctaaga
       61 tgttgtacaa tgtgaaagcg atcaagaacg attttagcat tcgggagtga aacagcctgg
      121 gagactgttt cagcctgagc ctagaaattt gaaagcgaag ctgtttagct aagtcatagt
      181 aaggactaaa catatccata gtaataattt tgacgcgaca tcggacggct ctatcatatt
      241 taagaaagtg atctcggatg acagcttgtg ttctgccttc aagaacagtg ataatattaa
      301 gattatcaaa atcttgcgca atgaagctca tctccatctc ccgattgaaa cagtcactcc
      361 ccggactgtt tcaacctccc aagacataat ctcaggcaga tttctaaaat tacactcaaa
      421 gtg
//
LOCUS       DAVMEF010000374          423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.380, whole genome shotgun sequence.
ACCESSION   DAVMEF010000374 DAVMEF010000000
VERSION     DAVMEF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.380"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            36..423
                     /locus_tag="VYQ30_002153"
                     /pseudo
     CDS             36..423
                     /locus_tag="VYQ30_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          146 a           79 c           83 g          115 t
ORIGIN      
        1 acttcctgcg aaacaaaata tggtataata gttctatgaa ttatgaagca agtaaacaac
       61 taaccgatgc acgatttaag cgtcttgttg gtgttcaacg cacgactttt gaagagatgt
      121 tagctgtatt aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat
      181 taagcctaga agaccttctt atggccactc ttcaatatgt gcgagaatac cgcacttatg
      241 aacaaattgc ggctgatttt ggtattcacg aaagcaactt aatccgtcgg agctaatggg
      301 ttgaagtaac tcttgttcaa agtggtttta cgatttcaag aactcctctc agttctgagg
      361 acacggtaat gattgatgcg acggaagtaa aaatcaatcg ccctaaaaaa acaattagcg
      421 aat
//
LOCUS       DAVMEF010000375          398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.381, whole genome shotgun sequence.
ACCESSION   DAVMEF010000375 DAVMEF010000000
VERSION     DAVMEF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.381"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(268..>398)
                     /locus_tag="VYQ30_002154"
     CDS             complement(268..>398)
                     /locus_tag="VYQ30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1967174.1"
                     /translation="EVLNLQLVSVQVDETDEVDEMRFSTFSTNRCGNWSAFSWENC"
BASE COUNT          137 a           90 c           49 g          122 t
ORIGIN      
        1 ttctgtctaa cttttggggt gcagttcata aaatcttgac ccgttaaatt tgtactgaat
       61 aaactaaatg aaacttttat cccactcact aatactatta tatcaaccca ctacagttga
      121 caaagagaca aaccagccta atggctaact tgtcttcaat caagagagga ttctgttccc
      181 ccatataagc actaacattc caacgtacac atattggaac gacatccata actccagaga
      241 atccctaaag tttacaattt aaatgaatta acaattttcc caactaaaag cactccagtt
      301 accgcaacgg tttgtactga atgtactaaa tcgcatttca tcaacttcat ctgtttcgtc
      361 aacttgaaca gatactaatt gaagatttaa tacttctt
//
LOCUS       DAVMEF010000376          394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.382, whole genome shotgun sequence.
ACCESSION   DAVMEF010000376 DAVMEF010000000
VERSION     DAVMEF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.382"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>394
                     /locus_tag="VYQ30_002155"
     CDS             <1..>394
                     /locus_tag="VYQ30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEV1967175.1"
                     /translation="TTDLLQLKSCIEKYSKSELGEGILQYAEICEKMSHIFNEEKQRY
                     LKVDLVNSYDSLLPKDLKKTLEDFVNFISRINLGKDYRNKELISYTEKFVEKYGEYVE
                     VPIKQLLDSKLGLGIPKQNLEPYSILSSV"
BASE COUNT          148 a           48 c           75 g          123 t
ORIGIN      
        1 taactactga tttgttacaa cttaaatcat gcatcgagaa gtattcaaaa tcagagttag
       61 gagaaggtat tcttcaatat gctgaaattt gtgaaaaaat gtctcatatt tttaatgaag
      121 aaaaacagcg ctatctgaag gttgacttag tgaatagtta tgattcgcta ttacccaaag
      181 atttaaaaaa gacattggag gacttcgtta actttatttc gagaattaat ctgggtaaag
      241 attatcgaaa taaggaatta atctcgtaca cagaaaaatt tgtcgaaaaa tacggagagt
      301 atgttgaagt tcctattaag caactactgg atagtaagtt agggttgggg ataccaaaac
      361 aaaatttgga accttatagt atattatcaa gtgt
//
LOCUS       DAVMEF010000377          394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.383, whole genome shotgun sequence.
ACCESSION   DAVMEF010000377 DAVMEF010000000
VERSION     DAVMEF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.383"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..263)
                     /locus_tag="VYQ30_002156"
     CDS             complement(<1..263)
                     /locus_tag="VYQ30_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967176.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYE"
BASE COUNT          131 a           80 c           41 g          142 t
ORIGIN      
        1 ttctcataaa atccacttgt atcgctaacc aatccaattc tctttaattc accgcttgtt
       61 aaattttgag atttttgtcc caaaataata gattgtcctt tatcggcaag gagctgccca
      121 gtcagaatat taatcgttgt ggtctttcca gaaccagatg gtcctaaaaa tccaaaaatc
      181 tgaccttctt taacagtgaa acttatatca tttaaagcta ttttatcacc aaaacttttt
      241 gataaatggc taacttcaat catactcatt gcccctcctt tatattctac atttttactt
      301 aacatctatt tacttagctt tatcaaataa acgtcttaaa aaaaacaata acattaagca
      361 tataaccgtt agcaagattg tttcaatatt attg
//
LOCUS       DAVMEF010000378          393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.384, whole genome shotgun sequence.
ACCESSION   DAVMEF010000378 DAVMEF010000000
VERSION     DAVMEF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.384"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(7..>393)
                     /locus_tag="VYQ30_002157"
     CDS             complement(7..>393)
                     /locus_tag="VYQ30_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HEV1967177.1"
                     /translation="IDIPKLQELATDVFHSSIYYLNSSEILNNAICHGRSGIMLLFNL
                     MYLDTGESQFKAISDNLFKEIVNKATDSEYIFVERDIYFRGVNYDEVIEYIDFGLLNG
                     VSGIVLALMAQRTGNASPLAEMFFMQ"
BASE COUNT          143 a           84 c           49 g          117 t
ORIGIN      
        1 aaaccctcac tgcataaaaa acatttcagc caaaggagaa gcatttcctg tcctctgcgc
       61 cattagtgca agaactatcc cagaaacccc atttaacaag ccaaaatcaa tatactctat
      121 cacttcatca taattcacac ccctaaaata tatatcacgt tccacaaata tgtattcaga
      181 atcagtggct ttatttacta tttccttaaa taaattatca gaaatagctt taaactgtga
      241 ttcccctgtg tctaaataca tcaagttgaa caaaagcatt atcccagagc gaccatgaca
      301 aattgcgtta tttaaaattt cactactatt aaggtaataa atagatgagt ggaatacatc
      361 agtagctaac tcttgtaatt ttgggatgtc aat
//
LOCUS       DAVMEF010000379          382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.386, whole genome shotgun sequence.
ACCESSION   DAVMEF010000379 DAVMEF010000000
VERSION     DAVMEF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.386"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          117 a           78 c           51 g          136 t
ORIGIN      
        1 gaatgacacc gctgtcatta tctttatcat atattttcca aattgaattg tcaaggaata
       61 acacaagaaa atattaaaat ttatactctt cggaaatctc ttcaaactag gtcagtgccg
      121 ccttgaccta agtctaggtt actgacttgg tcagttctat agtagactga aactaaaata
      181 gtacacctct acttctaaaa cattgttaga aatcgatttg actgtcccga tcaatttgtc
      241 ctattcttat ttcattttac tatatctaca acctcaaagc agggttttga gctatctatg
      301 gccaacttcc tagtttgctc tttagccttc cattcaaaag tgatgctatc tttcttagct
      361 tgaatataaa atatcccatt ta
//
LOCUS       DAVMEF010000380          381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.387, whole genome shotgun sequence.
ACCESSION   DAVMEF010000380 DAVMEF010000000
VERSION     DAVMEF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.387"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..>381
                     /locus_tag="VYQ30_002158"
     CDS             43..>381
                     /locus_tag="VYQ30_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV1967178.1"
                     /translation="MYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSQKIH
                     KYNSDVQVVPISIKVSSVEELENIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKIS
                     YISGGFNGCYL"
BASE COUNT          130 a           49 c           75 g          127 t
ORIGIN      
        1 ggagccggag ccttaggatg ttatatatct ctaagtctaa gtatgtatgg agtgaggaaa
       61 cttattgtcg ctgattacga tataatagaa ccatcaaatt taaataggca aattctttat
      121 acagagtcgg atgttggtaa ggagaagatt aatgttcttt ctcaaaaaat acacaagtat
      181 aattcagatg ttcaggtagt acctatttct attaaagttt cttcagtaga agaattagaa
      241 aacattgttg cggaatatgg gagtatagat tttatcgtta aagcaattga tacgcccatt
      301 gatattataa aaattgtcaa tcaatttgct gtatcgcata agatatccta catatcagga
      361 gggtttaatg gatgctatct t
//
LOCUS       DAVMEF010000381          364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.390, whole genome shotgun sequence.
ACCESSION   DAVMEF010000381 DAVMEF010000000
VERSION     DAVMEF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.390"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>364
                     /locus_tag="VYQ30_002159"
     CDS             <1..>364
                     /locus_tag="VYQ30_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1967179.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAA"
BASE COUNT          117 a           79 c           85 g           83 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagc
//
LOCUS       DAVMEF010000382          363 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.391, whole genome shotgun sequence.
ACCESSION   DAVMEF010000382 DAVMEF010000000
VERSION     DAVMEF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.391"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>363
                     /locus_tag="VYQ30_002160"
     CDS             <1..>363
                     /locus_tag="VYQ30_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1967180.1"
                     /translation="EEAKPGNEEKIKKAKAKVESEKAEAIRLEEIKTDREEAKRKADA
                     KLKEAVENNAATSEQGEPKRRVKRGVLGEPATPDKKENDAKSSDSSVGEETLPSPSLK
                     PEKKVAEAEKKVAEAEKKA"
BASE COUNT          155 a           56 c           98 g           54 t
ORIGIN      
        1 gaggaagcta aacctggaaa cgaggaaaaa attaagaaag caaaagcgaa agttgagagt
       61 gaaaaagcag aggctataag gttagaagaa atcaagacag atcgtgaaga agctaaacga
      121 aaagcagatg ctaagttgaa ggaagctgtt gaaaacaatg cagcgacttc agagcaaggt
      181 gaaccaaaga ggcgggtaaa acgaggagtt cttggagagc cagcaacacc tgataaaaaa
      241 gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc aagcccatcc
      301 ctgaaaccag aaaaaaaggt tgcagaagct gagaagaagg ttgcagaagc tgagaaaaaa
      361 gcc
//
LOCUS       DAVMEF010000383          360 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.392, whole genome shotgun sequence.
ACCESSION   DAVMEF010000383 DAVMEF010000000
VERSION     DAVMEF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.392"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>360
                     /locus_tag="VYQ30_002161"
     CDS             <1..>360
                     /locus_tag="VYQ30_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEV1967181.1"
                     /translation="TETPWCREGETEDEDYLDISIFEVFEPNDWLKIDNCKIDSVIAS
                     YQRLVDNKLKYEGADRTILKIVIAADMAEDKTKRELLLNWAQCLSDWNLKYSKNCEMA
                     IINDLQIKSRVRKLNSK"
BASE COUNT          137 a           47 c           71 g          105 t
ORIGIN      
        1 tactgaaact ccttggtgcc gggaaggaga aactgaagat gaagattatt tagacatttc
       61 aatttttgaa gtatttgaac caaacgattg gctaaaaatt gataattgta aaattgattc
      121 agttattgcc tcgtatcaaa gattggtaga taataagctg aaatatgaag gcgctgaccg
      181 tactatttta aaaatagtta tcgctgcaga catggcagaa gataagacta aaagagagtt
      241 gttacttaat tgggcgcaat gtctttctga ctggaactta aaatactcaa aaaattgtga
      301 gatggctata ataaatgacc ttcaaataaa atctagagtt cgtaaattaa acagcaaaga
//
LOCUS       DAVMEF010000384          338 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.395, whole genome shotgun sequence.
ACCESSION   DAVMEF010000384 DAVMEF010000000
VERSION     DAVMEF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.395"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          116 a           44 c           56 g          122 t
ORIGIN      
        1 atggagaaaa aaatgcttta atcattctta tcgttttcta tgctcaccta taattacaga
       61 tgattttctt aacacgaaga cattgagaag tagccaaatt gaatataaat atgagcgata
      121 tttatcgaaa agttcgatag gcgatagagc ggttgatggc tttgtttcct tcaatacttt
      181 aacagctaat ggtatgtctg ctattaaact atgtcttgag atattaaact ctattttctt
      241 caagaagaag attgatttat tatattcaac cggatattat gaaacaagat ttttattaaa
      301 taatcttgct aaatcaggta ttagttgcta tgaggtaa
//
LOCUS       DAVMEF010000385          328 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.396, whole genome shotgun sequence.
ACCESSION   DAVMEF010000385 DAVMEF010000000
VERSION     DAVMEF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.396"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..172
                     /locus_tag="VYQ30_002162"
     CDS             <1..172
                     /locus_tag="VYQ30_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1967182.1"
                     /translation="TVILTQKQNDSTFVQSIIGKELQAANIKVKDQAFQNIFTQYIGG
                     GDSSSSSSTSNE"
BASE COUNT          129 a           64 c           56 g           79 t
ORIGIN      
        1 aactgttatc ttgactcaaa aacaaaatga ttcaacattt gttcaaagca ttatcggaaa
       61 agaattgcaa gcagccaata tcaaggttaa ggaccaagcc ttccaaaata tctttaccca
      121 atatatcggt ggtggagatt caagctcaag cagtagtaca tcaaacgaat agtccaaatc
      181 aatgagtcag ggaaaaaact cgacttcagg aaaaaatgaa gcaaacattc ccacaataaa
      241 acgcatagta caaggtttgt actgcccccc aaaaaagtta ggcaattaat ttatccgaag
      301 gatttagttc tgtattgcac agagctaa
//
LOCUS       DAVMEF010000386          325 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.397, whole genome shotgun sequence.
ACCESSION   DAVMEF010000386 DAVMEF010000000
VERSION     DAVMEF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.397"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..95)
                     /locus_tag="VYQ30_002163"
                     /pseudo
     CDS             complement(1..95)
                     /locus_tag="VYQ30_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          107 a           70 c           47 g          101 t
ORIGIN      
        1 gagtagggag gaaaaggtaa aagtttatgc ccaaactctt cacataagaa ctctagctta
       61 cccattctat ggaatcttgc attatccatt aaaacttcca gactcatacc cttctaaaat
      121 ctattcaaac cacgtcagct tcacattata tatgttggtt acctcacaag ttttatctgc
      181 aacctcaaag ctgttctttg agcagcctac agctagcttc ctagtttaat ctttgctttt
      241 cactgagtat caagttaaaa aaatgaggct ggtcaaaaag tctttaaaat tataaaacgc
      301 atcatatcag gtgtacatga actac
//
LOCUS       DAVMEF010000387          310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.398, whole genome shotgun sequence.
ACCESSION   DAVMEF010000387 DAVMEF010000000
VERSION     DAVMEF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.398"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>310)
                     /locus_tag="VYQ30_002164"
     CDS             complement(<1..>310)
                     /locus_tag="VYQ30_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967183.1"
                     /translation="LLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEP
                     TSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPS
                     "
BASE COUNT           91 a           64 c           55 g          100 t
ORIGIN      
        1 tcagaaggag ctccttgctc aactaatttc cctttattta ataaggcaac ataatcacat
       61 aaaagagttg cttcattcat atcatgagtc gttagaaaaa tcgttgtccc tgctgttttc
      121 aattctaaaa ttaactcatg aattgttcga gaagttgtgg gatctagacc tgaggtcggt
      181 tcatccagaa agagtacagc gggtttgttg ataagagctc gtgctaaaag cattcgttgc
      241 ctcattccag tggataattt tcccgctacc atcttgcaac tatcatataa tcctactcgc
      301 tttaacaaat
//
LOCUS       DAVMEF010000388          306 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.399, whole genome shotgun sequence.
ACCESSION   DAVMEF010000388 DAVMEF010000000
VERSION     DAVMEF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.399"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>306)
                     /locus_tag="VYQ30_002165"
     CDS             complement(<1..>306)
                     /locus_tag="VYQ30_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967184.1"
                     /translation="LYRKPYIKSISAAITGAILLISSFQMISQNILSQFQDDSDLELK
                     VAFIVYVGAPILIVLANIISIAFLSSHQERTEIQQFEILGTSNYQMVKIKVGEAIFLT
                     "
BASE COUNT          111 a           51 c           43 g          101 t
ORIGIN      
        1 tgtcaaaaat atagcctcac caactttaat cttaaccatc tggtaattag aagttcctaa
       61 aatttcaaat tgctgaatct cagtcctttc ttgatgcgat gacaaaaatg caattgaaat
      121 aatatttgca agtactatca aaattggtgc tcctacatag acaataaatg ccacttttag
      181 ctctaaatca ctgtcatctt gaaattgaga tagtatattc tgagaaatca tttgaaaact
      241 agaaattagt aatatagctc ctgtaattgc agcactgata gattttatat aaggcttacg
      301 atatag
//
LOCUS       DAVMEF010000389          304 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.401, whole genome shotgun sequence.
ACCESSION   DAVMEF010000389 DAVMEF010000000
VERSION     DAVMEF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.401"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          120 a           58 c           36 g           90 t
ORIGIN      
        1 tagccaatat aactacaaat gaatcaagat tacaaaattc tatcattacc tgacaagaca
       61 ccctgcaaga tgctataaaa gatgataaaa tacctttttt tccgcctaca atacatattt
      121 ttgaaaacga acgttctcct ttaaatacag ctttgtaaaa atatactcgt tctgcacagc
      181 aagtaggaga aaagcaacat tttctatatt acatccagaa aaaaattttt atcttcagtt
      241 tctaacgcag ctccaacctt aaaataagaa taaggagcat aagcttggac cattgattta
      301 ataa
//
LOCUS       DAVMEF010000390          302 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.402, whole genome shotgun sequence.
ACCESSION   DAVMEF010000390 DAVMEF010000000
VERSION     DAVMEF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.402"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..289)
                     /locus_tag="VYQ30_002166"
     CDS             complement(<1..289)
                     /locus_tag="VYQ30_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967185.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQK"
BASE COUNT           71 a           59 c           53 g          119 t
ORIGIN      
        1 tcttttgttc aaagatagtc gtgataatct gacgcaattc ttcgcgttct ttttccggaa
       61 tctcaccact tgtttgagct acagcgtaga gttcagggtg ttcaattgaa atgcgtttaa
      121 tcgtacgtgt tgtagcatgt tttctgacaa aaaacgggat tcgcttaatc aagtcttgtg
      181 gtactagcgc cagaatcttc tcagtagttt ctttgtcact aatattagac attgtaagcc
      241 ttttcttaat catttcctgt tctttttctg taaaatcttt taattccatt cgattagtcc
      301 tc
//
LOCUS       DAVMEF010000391          297 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.403, whole genome shotgun sequence.
ACCESSION   DAVMEF010000391 DAVMEF010000000
VERSION     DAVMEF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.403"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..>297
                     /locus_tag="VYQ30_002167"
     CDS             27..>297
                     /locus_tag="VYQ30_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV1967186.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLY"
BASE COUNT          102 a           37 c           64 g           94 t
ORIGIN      
        1 ataaagaatt taaggagaaa tacaatatgt caatttttat tggaggagca tggccatatg
       61 caaacggttc gttacatatt ggtcacgcgg cagcgctttt accgggggat attcttgcaa
      121 gatactatcg tcagaaggga gaggaagttt tatatgtttc tggaagtgat tgtaatggaa
      181 cccctatttc tatcagagct aaaaaagaaa ataagtctgt gaaagaaatt gctgattttt
      241 atcataagga atttaaagaa acttttgaaa aattaggttt tacatatgac ttgtact
//
LOCUS       DAVMEF010000392          282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.404, whole genome shotgun sequence.
ACCESSION   DAVMEF010000392 DAVMEF010000000
VERSION     DAVMEF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.404"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>282)
                     /locus_tag="VYQ30_002168"
     CDS             complement(<1..>282)
                     /locus_tag="VYQ30_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1967187.1"
                     /translation="KDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTL
                     LQPNQIILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLN"
BASE COUNT           97 a           44 c           40 g          101 t
ORIGIN      
        1 atttaagtat gtgtcaatta ctatacttat ccattcattt gcctttgctt taaagaaaaa
       61 atcagcggca ggagcgtcca tcttacaatt taatatttcc attgccactc tttctaaggc
      121 ctttgtaagt attatttgat tcggttgaag caaggttgaa taaaaagatt ctggattaat
      181 gttaatagat gctaaatgtt tttctattag ctctttttta aaaccaatgg aaacagcaag
      241 ataacaacaa ttctcgtgta ataaaaaaac aaaatcatct tt
//
LOCUS       DAVMEF010000393          282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.405, whole genome shotgun sequence.
ACCESSION   DAVMEF010000393 DAVMEF010000000
VERSION     DAVMEF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.405"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>282)
                     /locus_tag="VYQ30_002169"
     CDS             complement(<1..>282)
                     /locus_tag="VYQ30_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967188.1"
                     /translation="IIALYCIVGSWNSFTPPLLYLNDDTKMPLSIYLRNLVVTNTTDT
                     AELSLSAGNTYYQQIAAQGGGIGMRIAIKMGTIVVSVIPIMMIYPFIQK"
BASE COUNT          110 a           48 c           41 g           83 t
ORIGIN      
        1 tgtttctgaa taaatggata aatcatcata attggaataa cactaacaac aatagtaccc
       61 atcttaatag caatacgcat accaatacct ccaccttgag cagcaatttg ttggtaatat
      121 gtattacctg cagaaagtga taattcagca gtatcagttg tatttgtaac aactaaattt
      181 ctcaaataaa tagataaagg catcttagtg tcgtcattca aataaagtaa tggaggagta
      241 aaactattcc atgaaccaac tatgcaataa agtgctataa tc
//
LOCUS       DAVMEF010000394          278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.406, whole genome shotgun sequence.
ACCESSION   DAVMEF010000394 DAVMEF010000000
VERSION     DAVMEF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.406"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           77 a           45 c           65 g           91 t
ORIGIN      
        1 aaggttaaat acctctattc gtagcgccta tagtgggtca gatattaccc cggtatattc
       61 attggggtct ggctctagga ttgtcatgta ctataatgga ggtggtgaca attatattgg
      121 ttctggtact agattagcta tggcgccaca atttggaaat catgtaagaa ttcatacttc
      181 aggttcttgg agtccagatt cttattaact tacttgtcag agtaagcctt aaagatggtt
      241 gattgtgggt gtagcatgaa aaaagaatgc tacaccct
//
LOCUS       DAVMEF010000395          278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.407, whole genome shotgun sequence.
ACCESSION   DAVMEF010000395 DAVMEF010000000
VERSION     DAVMEF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.407"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>278)
                     /locus_tag="VYQ30_002170"
     CDS             complement(<1..>278)
                     /locus_tag="VYQ30_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEV1967189.1"
                     /translation="YVASVVTVIAIGSGESALIATLIKIVRHEESYPYRDFANSFFKD
                     YWKNLGAALISNLPILILLFSFFFLPYIPLPIYIISILRHIGVIYIIL"
BASE COUNT           98 a           36 c           51 g           93 t
ORIGIN      
        1 tgaagtatga tatatattac tccaatgtgt cgtaagatag aaatgatata tataggtaat
       61 ggtatgtagg gtaggaagaa aaaactgaat aataagatta aaattggtaa attagatatt
      121 aatgctgctc ctaaattttt ccaataatct ttaaaaaaac tgtttgcgaa gtctctatag
      181 ggataggatt cctcgtgtcg tacaattttt attagagttg ctattagggc actttcacct
      241 gatccgatag caataactgt tactactgac gctacata
//
LOCUS       DAVMEF010000396          273 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.408, whole genome shotgun sequence.
ACCESSION   DAVMEF010000396 DAVMEF010000000
VERSION     DAVMEF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.408"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            45..>273
                     /locus_tag="VYQ30_002171"
     CDS             45..>273
                     /locus_tag="VYQ30_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV1967190.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT           88 a           35 c           82 g           68 t
ORIGIN      
        1 tttgtaacaa gacaaatatt aaaaataaaa aagaggtatt cgttatgaat acaaaaaaga
       61 tgtcacaatt tgaaattatg gatactgaga tgcttgcttg cgttgaaggt ggcggatgca
      121 attggggaga ttttgccaaa gcaggtgttg gaggaggagc agtacgaggt cttcagctag
      181 gaattaaaac aagaacatgg caaggtgcag caactggtgc tgcgggagga gctatacttg
      241 gaggtgtggc ctatgcagcg acatgttggt ggt
//
LOCUS       DAVMEF010000397          272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.409, whole genome shotgun sequence.
ACCESSION   DAVMEF010000397 DAVMEF010000000
VERSION     DAVMEF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.409"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..182
                     /locus_tag="VYQ30_002172"
     CDS             <1..182
                     /locus_tag="VYQ30_002172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967191.1"
                     /translation="INDDGDCFVCACRFLNPDRAEEYFCRGCQGENPEDIEFYDEQLQ
                     AEKVEALNIRLEVKN"
BASE COUNT           88 a           41 c           53 g           90 t
ORIGIN      
        1 caataaatga tgatggcgac tgttttgttt gtgcttgtcg atttttgaat cctgatcgag
       61 cggaagaata tttctgccga ggttgtcaag gggaaaatcc tgaagatatt gaattttatg
      121 atgaacaatt acaggcagaa aaggtggagg ctttgaatat tcgattggaa gtaaaaaatt
      181 gaattatcta caatctcatc aacaataaac ctatttcaac cccattatct ataggcgtat
      241 ttttacgcct ttttatatac ttgaagcaca ta
//
LOCUS       DAVMEF010000398          271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.410, whole genome shotgun sequence.
ACCESSION   DAVMEF010000398 DAVMEF010000000
VERSION     DAVMEF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.410"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            10..>271
                     /locus_tag="VYQ30_002173"
     CDS             10..>271
                     /locus_tag="VYQ30_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1967192.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSM"
BASE COUNT           99 a           40 c           51 g           81 t
ORIGIN      
        1 gaggaattta tggaaattat tgttccagat caaattatca tgggtttaat tttatatgct
       61 ggtgatgcga aacaacatat ttataaagcg ttagattaca taaaaaatgg tacatgtgaa
      121 cggtgtgaag aagaaataca gttagctgat gcagccttat tagaagctca taatctacaa
      181 acaaaattct tggcacaaga agcgtctggt acaaagacag aaattacagc tctctttgtt
      241 cattcacaag atcatctcat gactagtatg a
//
LOCUS       DAVMEF010000399          270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.412, whole genome shotgun sequence.
ACCESSION   DAVMEF010000399 DAVMEF010000000
VERSION     DAVMEF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.412"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>270
                     /locus_tag="VYQ30_002174"
     CDS             <1..>270
                     /locus_tag="VYQ30_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967193.1"
                     /translation="KTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTVTIQN
                     GKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV"
BASE COUNT          107 a           28 c           59 g           76 t
ORIGIN      
        1 aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac cgtaaaagaa
       61 ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag ggtaactgtg
      121 accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga ttttatttta
      181 cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga aatttctggt
      241 ttcgaaggta aaaaagacgc tggctatgtt
//
LOCUS       DAVMEF010000400          268 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.413, whole genome shotgun sequence.
ACCESSION   DAVMEF010000400 DAVMEF010000000
VERSION     DAVMEF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.413"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>268
                     /locus_tag="VYQ30_002175"
     CDS             <1..>268
                     /locus_tag="VYQ30_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV1967194.1"
                     /translation="YETYKPNVLHFCELLAEEEGIKLSDTTVRKILYKKNILSPKSHR
                     KTKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKKVHPSRPRKKF"
BASE COUNT          110 a           57 c           41 g           60 t
ORIGIN      
        1 ctatgaaaca tataaaccaa atgtccttca tttctgtgaa ttactagctg aagaagaggg
       61 aattaagctc tctgatacaa ctgttagaaa aatactctat aagaaaaaca tcctttctcc
      121 taagtctcac agaaagacaa agaagagagt aagaaaacaa gctaaactga acctgaatca
      181 acccctagac aatccaatct taccgacggc taaagacttc ctggaagacc ctaaaaaggt
      241 acatcctagt agacccagaa agaaattt
//
LOCUS       DAVMEF010000401          262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.414, whole genome shotgun sequence.
ACCESSION   DAVMEF010000401 DAVMEF010000000
VERSION     DAVMEF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.414"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>262
                     /locus_tag="VYQ30_002176"
     CDS             <1..>262
                     /locus_tag="VYQ30_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1967195.1"
                     /translation="SIHYVSTVTNCYKAAVDAYLESSEKFEAIKQDLVDEMWKVAQRE
                     LATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYDDAAQTAT"
BASE COUNT           77 a           52 c           63 g           70 t
ORIGIN      
        1 agtctattca ctacgtatca acagtaacca actgctacaa ggcggctgtg gatgcctatc
       61 ttgaaagttc tgaaaagttt gaagctatca aacaagactt agtggacgag atgtggaagg
      121 ttgcccaacg tgaattggca acaggtttct actacggtat accatctgaa aatgagcagt
      181 tgtttggtgc tcgccgtaaa atccctgagt acaagtttgt cgctgaagtg gtttcttatg
      241 atgatgcggc acaaacagca ac
//
LOCUS       DAVMEF010000402          262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.415, whole genome shotgun sequence.
ACCESSION   DAVMEF010000402 DAVMEF010000000
VERSION     DAVMEF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.415"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>262)
                     /locus_tag="VYQ30_002177"
     CDS             complement(<1..>262)
                     /locus_tag="VYQ30_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1967196.1"
                     /translation="SIHYVLTVTNCYKAAVDAYLESPEKFEAIKQDLVDEMWKVAQRE
                     LATGFYYGTPSENEQLFGARRKIPEYKFVAEVVSYDDAAQTAT"
BASE COUNT           73 a           64 c           49 g           76 t
ORIGIN      
        1 gttgctgttt gtgccgcatc atcataagaa accacttcag cgacaaactt gtactcagga
       61 attttacgac gagcaccaaa caactgctca ttttcagatg gtgtaccata gtaaaatcct
      121 gtagccagtt cacgttgggc aaccttccac atctcgtcca ccaagtcttg tttgatagct
      181 tcaaactttt caggactttc aagataggca tccacagccg ccttgtagca gttggttact
      241 gttaatacat agtgaataga ct
//
LOCUS       DAVMEF010000403          260 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.416, whole genome shotgun sequence.
ACCESSION   DAVMEF010000403 DAVMEF010000000
VERSION     DAVMEF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.416"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..210)
                     /locus_tag="VYQ30_002178"
                     /pseudo
     CDS             complement(1..210)
                     /locus_tag="VYQ30_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT           71 a           55 c           45 g           89 t
ORIGIN      
        1 ctcatagcca taaaacattt cggattttaa aatgccaaag aaagattcca tcctaccgtt
       61 gtcttggctg tttcctttgc gtgacataga tgcttgaatt cccttactct ctaggaagcg
      121 atgataagaa tcgtgttgat attgccagcc ttggtcacta tggagaatcg tattctcgta
      181 gtgcttctct ttgaatgcct gttccaacat taacgatcaa tcaatttaat catgtaccta
      241 agattagaat tgtttatccc
//
LOCUS       DAVMEF010000404          252 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.417, whole genome shotgun sequence.
ACCESSION   DAVMEF010000404 DAVMEF010000000
VERSION     DAVMEF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.417"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>252)
                     /locus_tag="VYQ30_002179"
     CDS             complement(<1..>252)
                     /locus_tag="VYQ30_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967197.1"
                     /translation="CIITVVLISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILL
                     LAGFSVIWLLSLQQIFQDSTILAKAQIIFNIVILDIVII"
BASE COUNT           98 a           46 c           31 g           77 t
ORIGIN      
        1 aaataatcac aatatctaag attacaatat tgaatattat ctgggccttc gctaaaattg
       61 tgctatcttg gaaaatttgt tgcaaagaaa gcaaccagat aacactaaaa ccagccaata
      121 gcagtattct ttttactatt gaaagaacat gccttatttt agaactcttc ctatttctaa
      181 tcttcttgaa cgtataaaag caaccactta gaaaggctaa aaatgaaatc aacactactg
      241 taatgataca tc
//
LOCUS       DAVMEF010000405          245 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.418, whole genome shotgun sequence.
ACCESSION   DAVMEF010000405 DAVMEF010000000
VERSION     DAVMEF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.418"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>245)
                     /locus_tag="VYQ30_002180"
     CDS             complement(<1..>245)
                     /locus_tag="VYQ30_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1967198.1"
                     /translation="DLISNFGDTLYFIALMTYVTEIKSSNLAISIVNISETIPILFTI
                     FFGIIADRTLNKVGMIIKTLWIRTILYLLVAVVMNFK"
BASE COUNT           88 a           46 c           31 g           80 t
ORIGIN      
        1 ccttaaaatt catgacaaca gccactaata aatataaaat tgtccgtatc cataacgttt
       61 tgataatcat tcccacttta ttaagtgtcc tatctgcaat gataccaaaa aatattgtga
      121 acaatatagg tatagtctcc gatatattta cgattgagat agccaaattg ctactcttaa
      181 tctccgtcac ataagtcatg agagctataa aatatagtgt atctccaaag tttgatatca
      241 gatct
//
LOCUS       DAVMEF010000406          219 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.420, whole genome shotgun sequence.
ACCESSION   DAVMEF010000406 DAVMEF010000000
VERSION     DAVMEF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.420"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           74 a           27 c           45 g           73 t
ORIGIN      
        1 acttccttta ttggaatgtc cttattttta ttatggaatt gccaaatgac gttaaggttc
       61 agttctatga acttagaaag aaagtacaaa gctttaatca gctttcaaaa agatttggta
      121 tggatgtttc tggctaaggt atgtgacaag gtgaattgat cgttattgaa cagagagcgt
      181 taaaaaaggg aagaatcgtt actattccac tgaattaaa
//
LOCUS       DAVMEF010000407          213 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E13
            SAMN29867833-rid17926553.denovo.422, whole genome shotgun sequence.
ACCESSION   DAVMEF010000407 DAVMEF010000000
VERSION     DAVMEF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867833
            Sequence Read Archive: SRR20340605
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720090.1
            Genome Coverage       :: 165x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,181
            CDSs (total)                      :: 2,134
            Genes (coding)                    :: 2,017
            CDSs (with protein)               :: 2,017
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 3 (5S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 55 of 117
            Pseudo Genes (incomplete)         :: 52 of 117
            Pseudo Genes (internal stop)      :: 34 of 117
            Pseudo Genes (multiple problems)  :: 22 of 117
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867833-rid17926553.denovo.422"
                     /isolate="GC089790_E13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-01-29"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>213)
                     /locus_tag="VYQ30_002181"
     CDS             complement(<1..>213)
                     /locus_tag="VYQ30_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967199.1"
                     /translation="RIRLKSIKHFTSFSFKLAALSTGIWTATLFLLIFLIAFSNGFSF
                     SLEIKEVDFLREFYGISIANNASFFIG"
BASE COUNT           87 a           42 c           27 g           57 t
ORIGIN      
        1 cctataaaga aactagcatt gtttgcaata cttatgccat aaaattctct taaaaaatca
       61 acctccttta tctccaaaga gaagctaaaa ccattactaa atgcaattag aaaaatcaat
      121 aaaaataaag tcgccgtcca aatccccgta ctaagagctg ctaatttgaa actaaaactg
      181 gtaaagtgct taattgattt cagacgaatg cga
//
